LOCUS       DBMDWL010000001        43469 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_369_length_43469_cov_3.632632, whole genome shotgun sequence.
ACCESSION   DBMDWL010000001 DBMDWL010000000
VERSION     DBMDWL010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 43469)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 43469)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43469
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_43469_cov_3.632632"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..2283)
                     /locus_tag="ACZPDN_00005"
     CDS             complement(100..2283)
                     /locus_tag="ACZPDN_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR1297042.1"
                     /translation="MQNSSNLSKSYKEIIKQHVFTLFNGINVILAIFVFFTGSYRNML
                     FMITVILNMLIGLFQEIRSKRMLDKLSLLNQPKIHVLRNDIELELSVEEVKVNDILIL
                     YPGDEISFDGHIVSGSIECDESMLTGESDAVYKQENSSLLSGSFVVSGKCLMKVDKVG
                     QDTYAYSILKHAKRFKRYPSQLRDSIDTIIKWCTYILIPLGCALFIKQFIKTNYTTAT
                     LNTVAAVVGMIPEGLVFLTSVALAISSFKLGKQSVLVQELYCIETLARTDTLCLDKTG
                     TLTQGKLSVCHVEEIKNIDGILGDMMQTLPDENATALALRSHFKARNHNKVVSFLPFS
                     SQRKYSSVTFENGEYKLGAYSYIAKEIDSKVEEHIEEYTSQAMRVIVLMKEDVVLAYI
                     CLRDELRDDAKDTLNFFKKQGVDIKLISGDDPKTVEALARKAGFTSKSIDMSKVQDVE
                     TVVDSYSIFGRVTPEQKKELVLALKKRNKTVAMTGDGVNDVMALKEADCSIAMGSGSQ
                     ACKSVASLVLLKDQMNALPAILYQGRCVINNIQRTASLFLVKTLFSIGLSLLALIFLK
                     NYPFKPIQLTLISALATGIPSFVLTLEPNGNKVKGNFLQNVISKALPGAICVICSVIG
                     VSILGHFIYISPSEYSTMCTILAGVNALMVLIRVCVPMTLLRKILVTIMCMIFVGSLI
                     LCKNFFYIVHLRWYEIIYVILNICLIPHILTVVSSWIKLFLKQKK"
     gene            complement(2356..2823)
                     /locus_tag="ACZPDN_00010"
     CDS             complement(2356..2823)
                     /locus_tag="ACZPDN_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297043.1"
                     /translation="MEYSALVFDDCKEVEDTYDKLLAIIDLFANDENCTQIVVSCKSV
                     WTLRLASSPKNKVMLVQRPDKPYVALLNGLKAVKQENVVVTGISTKHIKEDVDTLLDH
                     LSRYPAINFDKDLQAFDTRLLMFCLQKAIESNLAITSYAQAVTLANTPLESVE"
     gene            complement(2832..3479)
                     /gene="ispD"
                     /locus_tag="ACZPDN_00015"
     CDS             complement(2832..3479)
                     /gene="ispD"
                     /locus_tag="ACZPDN_00015"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR1297044.1"
                     /translation="MNYSVIVLAAGRGSRTNLDYNKVFYTFENGQTVLNKSLSVFLSD
                     DECKQVVLVCADYEKDYVNEHYTYDSRIQVVTGGQTRQDSVYNGLQVVDQDYVMIHDG
                     ARPFVEKNWIFDLKQTLETESACLLMVPSVDTTKIVVDGYVKESLERKLVYHAQTPQC
                     FKTDLIKECHKLARDKQVQATDDAQLVEWFSDTKVKVVLSSFTNKKITLPEDLKG"
     gene            complement(3530..4051)
                     /gene="nrdG"
                     /locus_tag="ACZPDN_00020"
     CDS             complement(3530..4051)
                     /gene="nrdG"
                     /locus_tag="ACZPDN_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1297045.1"
                     /translation="MYYGNIKKNDIANGKGVRVTLFVSGCTNHCKNCFQPETWNFDYG
                     KPYTKETEDEIIEALKPSHIAGLTLLGGEPFEPENQRELVKLLRRVRKELPEKNIWSF
                     TGFILDQDLLDGGRKHCEVTDEMLSLLDVLVDGPFKEEEKDITLAFRGSRNQRVIDMK
                     KSLKEQEVVELEV"
     gene            complement(4189..6156)
                     /locus_tag="ACZPDN_00025"
     CDS             complement(4189..6156)
                     /locus_tag="ACZPDN_00025"
                     /inference="COORDINATES: protein motif:HMM:NF018259.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HIR1297046.1"
                     /translation="MRLLNIPKMYKEELLYSYLLRLAEANGFTNPKTFFNHRGIFPDK
                     DDTKLRLDSLEDISFLLPRLSSYYTGAEIIADHTVLPAYLLFMSKVKQYLTVNAMVRK
                     KRDYPNIVGHIMAGYIDDIYVCPDCLKEGHYLRRGHHLYGVKICHKHRMPLMKIIKDR
                     KGMAERFEPVTGFDLEEQVKYAKFADELLKANISTDLYTVRQALGAKAKEEPDLEDRY
                     LCIMTKLGFENPEALASRMYKESTMMRNIILGERFNILMCCAYALFGDVESFRKAVEK
                     QGKYIVPEGYTVVDKRGVILELKHKACGTTFAATAYGIESGWSCPCCMEKDEGKIFDK
                     MIQISSNGRFTTNAKLTGWKEEMEFFDYRRRESFVNTPYQVLKMVPDDIKNGTCETTP
                     FDEVAAKVRSYGPFTLSSYTNGIEDVVITHDTCGHEFSERIHAFYKKPRCPVCKIRQD
                     KWLLRFVEERTSRQYSLLGFHNDEAELLDMNTREVFNIDKKNLLKSLGSGVSTNALPV
                     TKYNEVSLDGYDSMHAKVAVYLFTHYQENEDIDITKQQIENIDIEQLKIIFMILKKRG
                     YLVSRRIGIYRLNISSEKLKQKTAKSVIREYISETYKVGDTFTLKELLGKEDIKYRAF
                     AKALGVMRDNGEIDIVKPGVYKIKAFTVEYF"
     gene            complement(6353..6811)
                     /locus_tag="ACZPDN_00030"
     CDS             complement(6353..6811)
                     /locus_tag="ACZPDN_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297047.1"
                     /translation="MIARYKNYFCNIDMSQTPINIWAYRPLNGFEKGITRKGNVYYEK
                     NVDISEVDEIFEPGFSVKWHGQWCGIASYSIENDIVAIMSGDKEFANNYGMEQLERGV
                     YTKGISITECCEIRFRKLDYFTHEKVFRIISEDELKTLWTKMIAELIPPR"
     gene            complement(6825..7973)
                     /locus_tag="ACZPDN_00035"
     CDS             complement(6825..7973)
                     /locus_tag="ACZPDN_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297048.1"
                     /translation="MGLENNTREIEVQNQELRKTNKEQTERAHKELGKALDDLDTLDN
                     DAELNETDSIEDTKAQDDISLHLDSNSIIEDETSEQFETINNPVYNEVENNGFSINDV
                     NQAVDAKEAYEAEIRDIQGNEAEQNSDASFKKDETYTVNLNEIQSKFDSENFTVEKSY
                     DVRASKGTRELAAKGNSELVPMGEDTNNHPEASEIYSKEEAARLKVERDEVKHPDEKT
                     VMQKIIGVDTGKIEEDLKGYLDPKNNVTGEKTGAQVFGFVAKAEDSTPFTRTPEECYV
                     NLRTDYPGTPYKDPKQPVYVLRYTDGTNYEIPYGEQFGGNCKEGAPCTLNGYTGSSKH
                     LVPEYTVKKDENGNGAIITDGKIYRVSPDGTETAVAELDKKDKCFALV"
     gene            complement(8094..8477)
                     /locus_tag="ACZPDN_00040"
     CDS             complement(8094..8477)
                     /locus_tag="ACZPDN_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HIR1297049.1"
                     /translation="MSNSIINKEDVNTNYIAENYYIFTIDGNKVSTKKEFFDAVEILF
                     SFPTSCKNKVSRFDDWMMDLSWLDSDRGICIVIDNFEHFLRDDLEFKDLVMDDFRQDI
                     LPFWEETVLTTVKDGKTRKMDVVID"
     gene            complement(8470..9426)
                     /locus_tag="ACZPDN_00045"
     CDS             complement(8470..9426)
                     /locus_tag="ACZPDN_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012755.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HIR1297050.1"
                     /translation="MGIENNTHEIEVQNQELQRANREQIEQANKEHEELGKVLDGLDT
                     LDNEAELDEISGNEDISEQDDAASQLDNNDKIEDETSEQFETINNPVYNEVENNGFSI
                     NDVNQAVDAKEAYEAEIRDMSQDEYHDQKELDQRVEENGSEPNMTEPSVLGNDLHQGD
                     VSNKETDNRSRLNEPEQDAVDMERSTEEIPGNDEIEDSLSDVSTVDETNIQEVKLSEL
                     PDNVQESYRKYESNAWTGPHEDATPGTRGGLEWKNKDGDLPENDENGEPVTYKEYDVN
                     DKEPGSNRDAERFVRDNNENVYYTNDHYESFRVIDDEEEQNE"
     gene            complement(9445..10722)
                     /locus_tag="ACZPDN_00050"
     CDS             complement(9445..10722)
                     /locus_tag="ACZPDN_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1297051.1"
                     /translation="MISRIIGEAKETAKFISNNRKMFDPRKNIDKQTILYLIVGISWF
                     LPVYVNDGKAFHMHVTAMGGILPSNYPLPGILKWVLFFVLLILYLFVFISPLLLYINH
                     VLEKQENSGQYAKNNVIICLVYMGFYQYILAVQKNTTIITVVVIAILIYCTILNFSIA
                     EKNGVIIWNSKTKKADIDLYEKGKKPVIILSLFLIVAFIVLPVCSSEGKVSDSFFSIS
                     GPPEYAKTNISLLKLAKGFKYTLRYEGLFETTERTASLFGIPIVFFLLVILIAILIIH
                     YHNTRKQRPNMHLLNLNVLLSAIYLTLLSSEFIGLIIQQSSYMTIYFFSMLIMVIAAV
                     LLVKECKKIASVDFAQFEPLSNASKVASDKVTETTEVINQNFGKDLCEENEAKESSQK
                     PKQRFCTNCGAEIMEGSKFCTECGMPIENDGNE"
     gene            complement(10827..13955)
                     /locus_tag="ACZPDN_00055"
     CDS             complement(10827..13955)
                     /locus_tag="ACZPDN_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014044.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297052.1"
                     /translation="MDNKIDLVAIKEERDLMLRIGLSIADKFINKKYLIDLSDSEVME
                     LPDYEEEKRLIRLFRLDKLIYDENENINDKLISVYSALQEINSTVFLLVEGTANSTSF
                     YVGVKEKNNITIADKVLEKSFIGNFPGSTLTRINKASEIRRIIDNIGRTENNDLCKNV
                     SSITVVPASRDEDKEKFVQGLEKYIDTMQGQIYNALFIAEPVNKEQLEERKKGLEELY
                     SSLSPLAKVSLAYGENENSSVSKSVFDNFSTSVNESVSKTIGFSSNGNFGMNSGSNTS
                     GGLNLFGFGLNGGSSSGSSLGLSGGPSFSKTTSKGITDTAGHGSSYADTASTGSSNTM
                     TIEQENKAITNMLDKIDEQLERIKSCEAFGIWECAGYFVAESIETSAMAASTFKALMI
                     GDDTDVENSYFNIWSSENKSGNTKKVLEYLTCGRHPVIEIEPPQGMGNLYARQYVTPG
                     NYISGKELPLFLGVPHKSIAGLAISSVASFGRNVIVQSRTKESKKIRIGSIHHMGETE
                     KTEVKLDLDSFSSHCFITGSTGSGKSNTVYGLLEKFMENDIPFLIVEPAKGEYKDVFG
                     AVENINIFTTNSLIGQMLKLNPFRFDPGIHVLEHLDRLIEIFNACWEMYAAMPAILKD
                     AVEKSYIEKGWDLLNSIYLGEGEPVYPTFSDLMQVLPKVINESSYSADTKGDYTGALV
                     TRVTSLTNGISGQIFCDSYDIPDSTFFDENTIVDLSRVGSTETKSLIMGILVLKLSEY
                     RMANANESNSKLRHITVLEEAHNLLKKVDSSAAGGNVVKKSVEMICNSIAEMRTYGEG
                     FVIVDQSPTAVDIAAIKNTNTKILMRLPEKTDCQTAGNAVGLNDKQIAEMSKLSTGIA
                     VVMQNNWLEAVLTHIDAYSNKYEKKINPVLFDELRILRGNVIEELMNQYIRDKEISLE
                     HMYDVIENTEIGSLKKKEMKYCMKYTLSRLEKGRDIEFFTTSLLNISCTKNLIKTLES
                     KIKKNDQPDEYEIKEWKNRFRKELEKYISLPPAYNNSIIPYLVHAQNVEQNGIDYVAV
                     ERKLIEKMNKPF"
     gene            complement(13970..15487)
                     /locus_tag="ACZPDN_00060"
     CDS             complement(13970..15487)
                     /locus_tag="ACZPDN_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1297053.1"
                     /translation="MEQAKIQSDPFKEKFTFFCNGSKMTGKTDFQDYENSSLLDWADE
                     ISEKIGTAIYDDFNLSVCGEYFEKEFLEGIISVYASEDAAYKCLSLRKENHNNHIDAN
                     DRLRSLQGLAEKYNFSTEPFYKLQVFCKNKDFVESNAAYVAVDTPEKAFCIVDDDISA
                     LAAYRKSTARIILSTSESNKAECIGKNIDGKKQYIWNVPAIRKTEIFNSIVDYFSVIK
                     QIRTIADKISSAYSILDKQDQLLFTTCISVEPVYYAADIPELKIRDTYKPEVYLYPDK
                     TADVPFQMDSDNTAILEIRNNVLTPKSAGTANVIFYTVINGEKTIFDSKPVHISDICI
                     ESITIKPHRLELDVNEKKSISIIISPRNVSDSRITYTCSDSDVASLEAKSDGTFVITA
                     NKVGSCTFEFKAVKTGVVGKCIVEVASTFSKRTSDSMEYYRKGLLAFVVSVISGFITV
                     LMNPFKYVFFIVAGICAYNMLKALALKHLEENKCKNTYTVLAILAVLFVIVSMTI"
     gene            complement(15523..16554)
                     /locus_tag="ACZPDN_00065"
     CDS             complement(15523..16554)
                     /locus_tag="ACZPDN_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1297054.1"
                     /translation="MDWKDLLDDISDVVKEAGEKIDAKAKDNNIDLSRLKEVNINKVK
                     EVNLGKLKDVDLNKIKEASLGKLKEVDYEKLKTIGLSRLEQVNLSGITETISNLGQRM
                     TFGYQEITREEHLRVLKPLLKEVEEAARRVDSTIPEGVRVYVKYTDTQNIEKKVNLSN
                     KKPVDIIRALNENTLGITDTFFTLPVNAQKAVLGIGFGKLVYFLNGKVFKNESLSDEF
                     CFNIGYGNELCEYLDTLLGPRKEIDARIGNLQKMGCDYKMKSMDENDNSSDSEDEENN
                     EQTPVFINVTNAAEDSYDDTADNKEEPVTAIVNESLKTQDAKKTCASCNKVIPAGAKF
                     CPYCGNRIE"
     gene            complement(17035..17679)
                     /locus_tag="ACZPDN_00070"
     CDS             complement(17035..17679)
                     /locus_tag="ACZPDN_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297055.1"
                     /translation="MEKWWFFRDTKDARIDEQCKIAEQLVPDRRHWLGGEPQAIPGFR
                     FSRDLTELIYSDTEERLHKPSADRPIKKGDIVYVSHLYVLASEIPAIQGVIRTCHSSG
                     VDLRITSINYQDNNEHSDYALLNSVLNQISAHRKRRNTQKPNSEKRKPGRPSRNVHYW
                     TLTRDGRNVVQDYVNGLISFNNARAKLTNKTPDRRSVGEKMFQRIVREYKSEKD"
     gene            17971..19866
                     /locus_tag="ACZPDN_00075"
     CDS             17971..19866
                     /locus_tag="ACZPDN_00075"
                     /inference="COORDINATES: protein motif:HMM:NF018259.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HIR1297056.1"
                     /translation="MLISNRFPPLDDELSISYVMRLAFANGFPNETDFLRAFVSTDNG
                     LQHTNDFCLDTKALADKINIMDPIEQLAQTTVIPALWPLLPASMQDRHIRKAFNGCKR
                     FGSDKVNLLTPQLHYCPECMKQDNYLHRIHNVPGIYYCPFHKTILRNFHGNSEVIPLV
                     SYEAQAAYQELFIALLKKWIKADYRTSKQALVKRVRDMTSVPIYEYLSDILSEYGIDD
                     YSAARLSSSLNSYMPGVPMHILIMLFGTVFPSADLLMDYLAKVSELYDLEEVSPEEYD
                     LLNRTSLGVIHLRHKACGHSFYTTENSIREGWLCPKCDARLCDQDFFEQIVAHKGRGE
                     YRIKKGYRGLRYKVALEHSCGQLLEVSASKYLYYGAKCACTHTLLLSDADKQVRKAGN
                     FRLLSFSGTDKDATILSEDCGHTFVFHYTKFIQHPCCKACSPRCPDEDMFRKELYDLV
                     GNEYELVGSYTTRLAETMFLHKTCGRTFSMRPSHILTGHRCPCCIRQYRKSEFVEFVD
                     AYTCGRYEVKSFVGKSKALILDKSSGREMLDERCFIIQELTRPTPSSRYPHEKPSDKP
                     LLLASDRVLAAIQELYPDPDSMITTRGIQVSGLSTKQIGRIMANQLTKLNVLERIGPG
                     TYKYKER"
     gene            19872..20549
                     /locus_tag="ACZPDN_00080"
     CDS             19872..20549
                     /locus_tag="ACZPDN_00080"
                     /inference="COORDINATES: protein motif:HMM:NF020305.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HIR1297057.1"
                     /translation="MNRISNKTKENRGYGKGEGKNYKPYIQAHEFNSQGTAVTLRDWI
                     TGRSVELLSQGEAEYWHILRFNDDIIDIREQIPLPKEDTTVIAEEMGCEPAGKGRTTM
                     TSDFFIVYADQHMEVHSFKKNMRSIERQRTLELLNIEKTFWERKNVSFRMVFGTDIDH
                     ILASNIRVCSYYYEESSVFDETSCLKHLIIRKKIDVDLTKEKIGIPVLRDVYRKEIAA
                     WMKSREM"
     gene            20525..22813
                     /locus_tag="ACZPDN_00085"
     CDS             20525..22813
                     /locus_tag="ACZPDN_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297058.1"
                     /translation="MDEIQRNVNKGLCRGSILNVRSADRLKAGRYRVLNIRNGLVTLC
                     EMDVSSFKLNVQVLTYLQDMLYDGMIEIESEEKLVFDYEKLSEYQKNVYTRNLSIVNA
                     VADAYGPDYLDLATHKPKPLLKKFEDEYGLSNFTVRRIIRLYIQSGFNNSSLLDQAQF
                     MQKKQDNYSCYTKKTGRKCENVITGKCLTSEDIAHFAEALEKYKKGRKKTFKSIFLLM
                     SDKYYSKEITCDDGTQLIELLPASERPSLRQFTYYCSKHLSKEEKDRIKTSAMEQRNN
                     KRLLLSDSRYNVEGPADRVQMDEVEVDMSIVSSLDPSITVGRPIVYVMYDVFGSNILA
                     IGISFDNNSVLGMTNCFLNLAQNKVEYCARYGITISEDMWPSCYLPGRILVDRGAEYR
                     SYEAKRIFRELNIHRELAPAGSGSMKGLVEQWFHQMHSSQNPSLEKHGLIEKRHDSKH
                     HKEACLTIDDFTVMLINFVIHHNCSVLSDYPLTADMISKRVDPVPVEIFKYGCKKYGT
                     PKPISNIPQYYFSLMLPQSAKYDRRGIRCNDLFYINKNDHELLADMYNAHTKRIEFPV
                     RIDPRDVGSVYYIRNNRIMRASLNSDKTGNADVAGMTLSEWTLIIKSLRARRRSLEIN
                     KQQFDTYIEKVNELIVKNAKKVVTNQPTTQNLLENRAKEKHLESNNLRMFDYLNQYLD
                     EQFGETVSASMLEAEMPNTYEVLDCDIDGENTNIGGLSSDASEMTETRENSNDSDINI
                     AENPAAYLAKLLAEGWTPGGDK"
     gene            22816..24501
                     /locus_tag="ACZPDN_00090"
     CDS             22816..24501
                     /locus_tag="ACZPDN_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017437.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1297059.1"
                     /translation="MKCFNDTYEYADDVDPKTNQYGIVDFSKIEYVEARYVKHFDSEN
                     NGNPCIEALPRPRSLEECTLDYFKPILGYSMSEIQALPSGERIYRLNQLRKLRLPIAF
                     DYMLEVEMHNALIRSYENRYQFYDPEIPVWMAVDRSNGVKFPEQAKNDTCGKYITNKT
                     SSVSGWSLLGGSGTGKSSAIDILKSHYPQVIEHPLTKGNLKQIVFLHVSCPANSNFKV
                     LYSTIGREIDNALSYTTSVYQNLLSNSKDPISTVVQLIQNFCIGIIIFDEIQEIDFAG
                     TKSNTFASLVTIGELSGVAIAVSGLESAYSKMFKKVYTARRVGKVISSNRYCFDKSSL
                     KTLMSLIFSYQWFDKKVSLTDEIVDRFELYTGGRIDQLITLYSYMNYEYLISPENKRP
                     EINGKFVDLCIEKYYGDMRRLLAEATIYHKKNGDFDGRMLEAFEKAKQNLNDLLGDDE
                     QADMAEHLSSQETLQAMNSFANTQSNVVQNVQKLFPQFEISQIIETLKTVYNEPGSSC
                     LDDSVLSGRVIQVLLGTTNSSLEDTSKKPKIAKKKRPDANLLKQNLLNVYHQQ"
     gene            complement(24645..26819)
                     /gene="nrdD"
                     /locus_tag="ACZPDN_00095"
     CDS             complement(24645..26819)
                     /gene="nrdD"
                     /locus_tag="ACZPDN_00095"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1297060.1"
                     /translation="MNVIKRSGEEVVFDSIKIENAIKKANKATTHNQMSDELIRSVTQ
                     SVIDKCENLKRSPNVEEIQDMVEGELMEHRCFAVAHNYITYRYERALVRKANSTDKQI
                     MSLLERNNEEVKQENSNKNPLVNSVQRDYMAGEVSKDITKRFLLPQDVMEAHEQGIIH
                     FHDSDYFAQHMHNCCLVNLEDMLQNGTVISETMIEKPHSFSTACNVATQIIAQVASSQ
                     YGGQSITLSHLAPFVQVSREKARREVKEELESLNLDSSEDTVNKMAEMRVRKEIEKGV
                     QMIQYQVITLMTTNGQAPFVTVFMYLNEVPEGQTRDDLAIIIEEMLKQRIKGVKNEKG
                     IWITPAFPKLIYVLEENNIEEGSKYWYLTELAARCTAKRMVPDYISEKKMLELKVDAN
                     GEGHCYPCMGCRSFLTTYLDENGKPKYYGRFNQGVVTLNLVDVACSSKQDEEAFWRIM
                     DERLALCKKALMCRHERLLGTPSDVAPILWQNGALARLKKGEPIDKLLFGGYSTISLG
                     YAGLCECVYYMTGHPHTEEPGTSFGLKVMQYLNDACAKWKAEENIDFSLYGTPMESTT
                     FKFSKHLQERFGIIPHVTDKNYITNSYHVHVTEEIDAFTKLSFESQFQKLSPGGAISY
                     VEVPNMENNIDAVLAIMKHIYNHIMYAELNTKSDYCQVCGYTGEIKIVEDENHKLVWE
                     CPNCGNRDQEKMNVARRTCGYIGTQFWNQGRTQEIKERVLHL"
     gene            27141..27839
                     /locus_tag="ACZPDN_00100"
     CDS             27141..27839
                     /locus_tag="ACZPDN_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR1297061.1"
                     /translation="MDYKIKGDSDCPIVEINLQNNETIKIERGSMAYMSNVTIEGKMN
                     SNKKGLGGVLGAIGRSLTSGESMFITEATGTSHDGLLGIAPSIPGKIVCLEVNSQNHY
                     YLNNGAFLACDNSVSYEMKTQSVGKALFGGTGGFFVMHTFGQGDLLINAYGDIMEINV
                     DDAHPITIDNEHVVAWDDSLDYEIKIASGTFGFTTGEGLVNVFHGNGKVYIQSRNLHS
                     LADALTPFIPQTKD"
     gene            complement(27863..28261)
                     /locus_tag="ACZPDN_00105"
     CDS             complement(27863..28261)
                     /locus_tag="ACZPDN_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1297062.1"
                     /translation="MQLTQSQEDYLETIYVLKSQQDHVRAIDVAAALGFSKPSVSHAL
                     KDLKEKELIYTDKTGYIFFTEQGQLQATQVYERHVYFKNQLLHAGVEPILAEEEACRM
                     EHTISEESFKKLKGNKCVRSCIDCKGDGCK"
     gene            28394..29326
                     /locus_tag="ACZPDN_00110"
     CDS             28394..29326
                     /locus_tag="ACZPDN_00110"
                     /inference="COORDINATES: protein motif:HMM:NF017967.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HIR1297063.1"
                     /translation="MFKCNKLLGASLAVVMGVSACATNVFAIDTSVIDEKWGKPTVVY
                     GGGLTNDQIQSTRDLFDIEDTNNVYETSVDANDLYNYLGIAGGDTSSLISSVMVQKQD
                     AGKGVKVKIITEDNITKITSNQYANAAITAGVSDVEIDVASVSKVTGESALTGVYKAL
                     EANGETLDLDRTQVAQDELETTNEIAENNADNSNFDSGRLDQAMVEIKQELAEIKKNQ
                     GSAATAEQVEQVVTDALKKFNLSDIISQDDIDKLIAFAKKYQNTSAIDSQEVLEQLNK
                     LSGNLKNQLSGLINKAQSEGWFDKIINFIKSLFN"
     gene            complement(29339..30529)
                     /locus_tag="ACZPDN_00115"
     CDS             complement(29339..30529)
                     /locus_tag="ACZPDN_00115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1297064.1"
                     /translation="MENFKFYAPTKVYFGKDEEKRVGKILKEFHPKKVLIHYGGQSAI
                     KSGLLDRVKKALDEENIAYCLCGGVVANPLLSKVNEGIRICKEEGVDFILAVGGGSVL
                     DSSKGIAYGVYNEGNVWDYYARKKKVKGALPMGCILTLAATGSEMSNSSVITNEDGNL
                     KRGLSSEYGYFKFSILNPELTYTVSKYQTMCGCTDIIMHTFERYFTPYDTLDLVDQMA
                     ESLVRTVIKNAKILLEDPTNEKARAEIMWAGSVSHNDMTGDRSLGDWACHQLEHELSG
                     MFNVAHGAGLAAIWDSWANHVYMENPTRFAKLGHRVFGLDDSDTLSCALNTIEKMEEF
                     FKMIGMPTSIQELLKRKCTEQELYELTYKCSFEHTRTIGKFKELDETDMLEIYRMANE
                     KEVA"
     gene            complement(30539..31123)
                     /locus_tag="ACZPDN_00120"
     CDS             complement(30539..31123)
                     /locus_tag="ACZPDN_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015674.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR1297065.1"
                     /translation="MKWFDSDYDYIDEFTTQWNERYKKIKRIGIILSVVLILIGILCF
                     VFPEKVFTTIQIIAAIGLIVWGACEIYSYITTTFFFRDAMWLASGILNILLGCLLCTM
                     PVTITVSTLTFLFAFVLLVNGIQKITWGSRLSFFRVINTNYLSLTGWVNLLLGIIFMV
                     MPFQSALVINYILAAYLIVAGISLLIEVVSTKRI"
     gene            31270..31746
                     /locus_tag="ACZPDN_00125"
     CDS             31270..31746
                     /locus_tag="ACZPDN_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR1297066.1"
                     /translation="MLKKYLIVFLVSMVPLIELRGAIPIAAGMGLPFVQYYIIAIIGN
                     MIPVPFIYFFARKILVWGADKPVIGNFFTWCLEKGEKGGKKLQETAGKGLFVALLLFV
                     GIPLPGTGAWTGTLAASILDMDFKSSVLAVMLGVILAGIIMALASMGLFGALGALF"
     gene            complement(31800..32738)
                     /locus_tag="ACZPDN_00130"
     CDS             complement(31800..32738)
                     /locus_tag="ACZPDN_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR1297067.1"
                     /translation="MLEFYKTFEDGTRKIQEPEPGCWINAIAPTEEERNYLIEQIGVL
                     PEFVKSSLDEEESSHMDYDDDFRQLLIIFDYPSAENEDDDSIDSTYSTLPIGIVILKG
                     YVVTISLYENWSILEMSQGRIKGMDTRLKTRFFLLLALRISQRFLICLRQIDRLSSRA
                     ETRLHQSVENDELIEMLGLEKSLVYFSTSLKSDEVTLNKIMRGKQIQLYEEDEDLLED
                     VMIEIHQAIEMCNIYSNTMAGTMDTFASIISNNMNIVMNKLTVITIVMAIPNIIFGFY
                     GMNVQLPFPFVWFPSFVAILACVVATVYFFRHNMFK"
     gene            complement(32918..33373)
                     /locus_tag="ACZPDN_00135"
     CDS             complement(32918..33373)
                     /locus_tag="ACZPDN_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1297068.1"
                     /translation="MELLEAKKIMDEKLGDYKIMALASCVDDYPMVRNVSCVFYNDKI
                     YFKTDKNFRKTQQLFKNDRVAMCFNGVQVEGTAKNLGLVIDEPGRIFEKKYKEFLWQS
                     YNAYSHVDTEILIEVTPKFVEIWDEDKDRNAFQLFIDFDKETVEYKPYD"
     gene            complement(33381..34019)
                     /locus_tag="ACZPDN_00140"
     CDS             complement(33381..34019)
                     /locus_tag="ACZPDN_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1297069.1"
                     /translation="MSSTKKLTTIAILSAMAMVLNLLIQFPIVPAVSFLSYDPKDIII
                     VIAGFIYGPMTSFIMSLLCSFLEIVFRGGNLLDVLMNVISTCTFACVAACIYKYKHSK
                     KGAIAGLVLGTVCCTLSMTLWNYIITPIYYQMPRSAVVGMLLPGIVPFNIIKCTLNAA
                     CAMLIYKPLVNALRNTQFVPQSNTNETLTKDVGIVSAFIIVSIVVLILVMKG"
     regulatory      complement(34107..34220)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(34295..34636)
                     /gene="rnpB"
                     /locus_tag="ACZPDN_00145"
     ncRNA           complement(34295..34636)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPDN_00145"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(34640..34981)
                     /gene="gpsB"
                     /locus_tag="ACZPDN_00150"
     CDS             complement(34640..34981)
                     /gene="gpsB"
                     /locus_tag="ACZPDN_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HIR1297070.1"
                     /translation="MATDMNLTAQEIYEKEFHVDLKGYAPAEVDEFLDMVIEDYQKYD
                     EKVEELGAAVTRYEEKIKELQQQLFALQSENENLNEKVNSDFVNGSSNTVDILKRIAR
                     LEKAVFNQSEE"
     gene            35086..35679
                     /gene="recU"
                     /locus_tag="ACZPDN_00155"
     CDS             35086..35679
                     /gene="recU"
                     /locus_tag="ACZPDN_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIR1297071.1"
                     /translation="MFLMSTIKYPNGISTINTMQSKSYSSRGMRLENDINDSNKYYRE
                     LDKALIYKKPTPVQVVHVDYPKRQRAKITEAYYKTPSTTDYNGVYRGKYIDFEAKETK
                     NKTSFPLFMIHPHQIKHLKKVVFHGGIGFFIIRFNYHNETYLVDSKMLIDKIENIDKS
                     SIPYTWFQNHGTLIQEGLYPRLAYLKVLDELYFKEDK"
     gene            35680..38136
                     /locus_tag="ACZPDN_00160"
     CDS             35680..38136
                     /locus_tag="ACZPDN_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1297072.1"
                     /translation="MAQPQNEQKKPTAPKTNRRKLDIWNKLAVSILSIFLVGCISVFF
                     VLVNIINDPDGMRFSQDGLTTLSNSRLYDSSGNLFYELGDEIREDVTYSQIPQSVVDA
                     FLSIEDSRFFDHNGFDLPRFLKSAMANLKSGSFSQGGSTLTMQMIDNAFTKNQEKKIE
                     TEQGSVSTVQKLKLKVQEIYLSLIAEQSINKEQIFEYYVNRIWFGSGNNTRGIQKAAQ
                     YFFNKDVSQLNLGEAAFLAGCINAPDTYNPLNNLNEANTTTDHLKAAQNRRNVTLELM
                     MQHGYITEEEYNLASNQDISFSLQYIERTSDDPNQAYITQTLSEVMELTGQDPAIIPM
                     DIYTALNQDAQKQADEICNGNIVQYPDEAFDVGFSMIENDTGEIIAVGPGRRYHSDSV
                     KIDNSTDSKQPGSTMKPLLAYSSTFDILGWSTVHQVNDKKKDYWKNGSYAPVNSDGKY
                     NGKMSLQDALGVSKNTTAAQAMIDLVTAKGYDYWIDFCKKLGYDEDVANAFNEQYAIG
                     GSGMYASTVQQASAYSMLANGGKRVDAHRIRKIIRRSDKKEFETKAQTHELISEQAAW
                     MMSQLLEKVVSGGYNNYNNILMSNYTVYGKSGTSDWQNEGAQYGIPAGTAKDEWSLGY
                     TNKYTIACWSGYTTEYIQQGYYITIYDLNVATAFHISHYMLDYMQKYATYSAIERPSG
                     ISDYKNGYIKDEFKSKGDTASESTDAQEACVADGGEWDEEAGTCKKADDEEKEACVAD
                     GGTWDGTTCKKEDTETNDAETTCVNNGGTWDGSACTYPTTPDPEPTPTPDPEPTPDPE
                     NPTGYVWYDRRSLYNVFDRL"
     gene            complement(38187..38912)
                     /gene="pflA"
                     /locus_tag="ACZPDN_00165"
     CDS             complement(38187..38912)
                     /gene="pflA"
                     /locus_tag="ACZPDN_00165"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR1297073.1"
                     /translation="MLGAIHSIETFGLVDGPGVRYVIFMQGCHMRCKFCHNADTWKVK
                     EPNQSAEAVLKKALRYRPYWKENGGITVSGGEPLLQIDFLIELFTQAKKEGVHTTLDT
                     CGQPFTFEEPFFSKFKQLMKVTDLVLLDIKHIDEKAHKELTGHTNQNILEFAKYLSDI
                     QKPVWIRHVLVPTINTDEVYLKRLHQFVAQLKNVERVEVLPYHTLGAYKWKELGLEYP
                     LEGIPTPSDQQLQIANTILETGK"
     gene            complement(38941..41178)
                     /gene="pflB"
                     /locus_tag="ACZPDN_00170"
     CDS             complement(38941..41178)
                     /gene="pflB"
                     /locus_tag="ACZPDN_00170"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIR1297074.1"
                     /translation="MKEQWQGFKGSKWQDEVDVRDFIQNNYKPYDGDESFLEGPTEST
                     NTLWEKLQKLQKEERAKGGVLDMETEVVSSLTAYGPGYLDKDLEKVVGLQTDKPLKRA
                     FMPYGGIRMSEEACETYGYKPSEKLHEIFTKYHKTHNDAVFSAYTPEMRLARRNKIVT
                     GLPDTYGRGRIVGDYRRVALYGIDYLIEQKRKDFAYCGDGTMSDDVIRQREELAEQIK
                     ALKEMKVMAASYGYDISQPAKNSLEAVQWLYFGYLAAIKTQNGAAMSVGRISTFLDIY
                     FERDLENGVFTESEIQEIVDHLTMKFRMVKFARIPSYNQLFSGDPVWATLDIGGLGMD
                     GRSMVTKTCFRFLHTLENMGPSPEPNLTVLYSSNLPEPFKKYASKVSIDTSSVQYEND
                     DVMRPVWGDDYAVCCCVSATQTGKEMQFFGARANLAKCLLYAINGGIDEKTKVQVGPE
                     YKPITSEYLDYDEVMHKYDIMMDWLSGLYVNILNLIQYMHDKYYYEASQMALIDTDVR
                     RTFATGIAGFSHVVDSLSAIKYAKVKTIRDEDGLVVDYEIEGDFPRYGNDDDRADEIA
                     VWLLKTFMRKIEKHHTYRDSEPTTSILTITSNVVYGKATGALPDGRKAGEPLSPGANP
                     AYGAEQNGLLASLNSVAKLPYEYALDGISNTQTINPDALGHSEEERVNNLVNVLDGYF
                     DQGAHHLNVNVFGVEKLKDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVISRT
                     CHKSL"
     regulatory      complement(41282..41358)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(41412..42281)
                     /locus_tag="ACZPDN_00175"
     CDS             complement(41412..42281)
                     /locus_tag="ACZPDN_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1297075.1"
                     /translation="MNKTKELMVQMMWELVGSIFVAIGIYNFAVQAKFPMTGFSGISI
                     IFYRLFHIPIGFSTILLNIPVAILCYKLLGKKFFIRSIRCMIVSSLIIDYVAPLFPVY
                     QGDRLLAALCTGVFGGIGYAMIYTRNSSTGGSDFIIMAVKALRPHLSLGNIAFLSDVG
                     IILVGGILFKDVDGMIYGMIVNLLFAVVVDKMIYGLNSGKVAFIVTKQGKRICQVIDE
                     TSQRGSTILNAQGGYKQDDMQVVMCACNSKEMYQVRKAVQEEDPESFFVVVESHEVHG
                     EGFKMTNIGEAQS"
     gene            42597..>43469
                     /locus_tag="ACZPDN_00180"
     CDS             42597..>43469
                     /locus_tag="ACZPDN_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297076.1"
                     /translation="MDTKKIGAFIAFNRKKKGYTQEQLAEKLGVTNKTISRWENGHYM
                     PDLSLLEPLSKELDITLNELLAGEEIVKEEAMEYSEQNLIQTIDYTDKKIKNEHKKIS
                     LFIIGIGILISLCAFTVFPSESSWGSIYSMIGLFLFVVGIFRELKITSLLKKGLISTI
                     LFILLLGIFFIFDYASVSQFKQPPIYRLTTTTVFSDDGNKMIEYQNPFYNVFRINADT
                     PNEYYLIDNKRQYTIDTVPTSPFNVDKSSMKQLKHYKSHYIGDNSNTSHLLSALPLSE
                     YGYEFEIDSENYGLT"
BASE COUNT        13566 a         8751 c         6820 g        14332 t
ORIGIN      
        1 aacatattat accaaaaaag accgatattc agatttttaa atcttgaata ttcggtcctt
       61 ttttattaag ctgaaacttc tttatccgtt acagctcctt tatttttttt gtttcaaaaa
      121 taattttatc caagacgata caactgttaa aatatgtgga attagacaaa tattcaaaat
      181 cacatagata atttcatacc atcttaaatg gacgatgtag aaaaaattct tacataagat
      241 taatgagcct acaaatatca tacacatgat agtaactaat atttttctca aaagtgtcat
      301 cggtacacat acgcgtatca ataccataag tgcattgact ccggcaagaa ttgtacacat
      361 cgttgaatat tcacttggcg aaatatagat aaaatgacct agaatcgata caccaattac
      421 gctacatata acgcatatag ctcctggcaa tgcttttgag atgacatttt gtagaaagtt
      481 acctttcact ttatttccat tgggttctaa tgttaataca aaactaggta ttcctgttgc
      541 caaagcggaa atcaaagtta attgaatagg tttgaatgga taattcttta aaaatatcag
      601 cgccaacaag gatagtccaa ttgaaaatag tgttttaact agaaacaaag atgccgttct
      661 ttgaatatta ttaatgacac atcgtccttg atacaaaatg gcaggtaatg cattcatttg
      721 atctttcaat aaaactaaac ttgctacact tttacaagct tgtgaaccag atcccatcgc
      781 aatcgaacaa tccgcttcct ttaatgccat gacatcattg acgccatctc ctgtcattgc
      841 gactgtttta tttcttttct ttaatgccaa aacaagttct ttcttttgtt cgggagtcac
      901 acgtccaaaa atcgaataag aatcaactac agtttccaca tcttgtactt tggacatatc
      961 gatactttta gatgtaaagc cagcttttct agctagagct tcaactgttt ttggatcatc
     1021 tcctgaaatc aacttaatat ccacgccttg ttttttaaaa aagttcaatg tatctttggc
     1081 atcgtctctt aactcatctc gcaaacatat ataagctaaa acaacatcct ctttcatcaa
     1141 cacaataacg cgcatagctt gagatgtata ttcttcaata tgttcttcta cttttgaatc
     1201 tatctctttg gcaatataag aataagcacc gagtttatat tctccatttt caaatgtcac
     1261 acttgaatat tttctttgac ttgaaaatgg cagaaaagat acgactttat tatgatttct
     1321 tgccttaaaa tgacttctta atgcaagtgc tgtcgcattt tcatcaggta aagtctgcat
     1381 catatcgcct agaattccat caatattttt aatttcttct acatgacata cacttaactt
     1441 tccttgtgtc agtgtacctg ttttatctaa acaaagtgta tccgttcttg caagtgtttc
     1501 aatacaatag agttcttgta ctaaaacgct ctgtttacca agtttaaagg aactgatggc
     1561 taaggcaaca cttgttaaaa agacaaggcc ttctggtatc ataccaacaa cggctgctac
     1621 tgtatttaaa gtagctgttg tataattggt cttgatgaat tgtttaataa acaaggcaca
     1681 tccaagtgga attagaatgt atgtacacca tttaataatg gtatcaatcg aatctcgtaa
     1741 ctggcttgga tatcttttaa agcgtttcgc atgttttaaa atcgaatagg catatgtatc
     1801 ttgtcctact ttgtctactt tcatcaaaca ttttcctgaa accacaaagc ttccactaag
     1861 caatgatgaa ttttcttgtt tatataccgc atcactttct cctgtcagca tggattcatc
     1921 gcattcaata cttccagata cgatatgacc atcaaaactg atttcatccc caggatataa
     1981 tataagaata tcattgactt tcacttcttc cacacttaat tcaagttcaa tatcatttcg
     2041 aagcacatga atctttggct gatttaataa ggaaagcttg tctagcattc gtttagatcg
     2101 tatttcttga aagagtccaa tcaacatatt taaaataacg gtgatcataa aaagcatgtt
     2161 gcgataggaa cccgtaaaaa atacgaatat agccaatatt acattgattc cattaaataa
     2221 agtaaataca tgttgtttaa tgatttcttt ataacttttt gataaatttg atgagttttg
     2281 catataccta ttgtactacc tttaagcatg caaaataatt aagtttttat taaaaatgat
     2341 acgactatag ccgtatcatt caacagattc aagtggagta tttgcaagcg taacggcttg
     2401 ggcataagat gtaattgcca agttagattc aatcgctttt tgtagacaga acatcaataa
     2461 tcttgtatca aatgcctgta aatctttatc gaaattaatt gctggatatc ttgatagatg
     2521 atccaataat gtatctacat cttcttttat atgtttagta gaaatacctg tcacaacaac
     2581 attttcttgt ttaacagcct ttaaaccatt caataaagcc acataaggct tgtccggtct
     2641 ttgtacaagc atcaccttat ttttaggtga tgaagcaagt cttagtgtcc atacactttt
     2701 acaacttaca acaatttgtg tgcagttttc atcattcgca aacaaatcga ttattgctaa
     2761 taatttatca tacgtatctt ctacttcttt acaatcatca aataccaaag ctgaatattc
     2821 catctttata cctatccttt caaatcttca ggtaaagtta tttttttatt tgtgaaactt
     2881 gataaaacaa cctttacttt tgtatcagaa aaccattcta caagttgtgc atcatctgtt
     2941 gcctgcactt gtttatctcg tgcaagctta tgacactctt taatcaaatc agttttaaag
     3001 cattgcggtg tctgcgcatg atatacaagc tttctttcta acgattcttt cacatatcca
     3061 tccacaacaa tttttgtagt atccacactt ggaaccatta ataaacaagc tgattcggtt
     3121 tctaatgttt gtttcaaatc aaaaatccaa ttcttttcaa caaaaggtct agccccatca
     3181 tgaatcataa cataatcttg atcaacaact tgtaagccat tatatacact atcttgtcgt
     3241 gtttgccctc ctgttacaac ttggattctt gaatcatatg tataatgttc attcacatag
     3301 tccttttcat aatcagcaca aactaaaacc acttgtttac actcatcatc tgataaaaaa
     3361 acagacagac tcttatttaa cacagtttgt ccgttttcaa aagtataaaa tactttatta
     3421 taatctaaat ttgtacgact gcctcttcca gcagctaaaa caatcacact atagttcatg
     3481 aaaccattat atcaaatttt cttaacaata gttaaaaaaa attggaagat taaacttcca
     3541 attctacaac ctcttgttcc ttcaaagatt tcttcatatc aataaccctt tgatttcttg
     3601 aaccacgaaa cgccaatgtg atatcttttt cttcctcctt aaatggacca tccactaata
     3661 catctaataa agacaacatt tcatctgtga cttcacaatg ttttcttcct ccatctaata
     3721 agtcttgatc caaaataaat ccagtaaacg accaaatatt cttttcaggt aattctttac
     3781 gaacacgacg aagaagtttc acaagttccc tttgattttc tggttcaaac ggttcaccac
     3841 caagtaatgt caaacctgca atatgactcg gttttaaagc ttcaataatt tcatcttccg
     3901 tttcctttgt ataaggctta ccataatcaa aattccatgt ttctggttgg aaacaattct
     3961 tacaatgatt tgtacatcct gatacaaata gtgttacacg tacgccttta ccatttgcga
     4021 tatcattctt ttttatattt ccgtagtaca tttttgggtt acctcctaaa aaagagctag
     4081 tgttaatctt caactccaaa tctcgttaag tcctttcttt tacataaata taacaacata
     4141 atctacaatt gtaaatatat tttctaaaaa cagagccctg agtgtgtatc agaaatattc
     4201 aactgtaaat gcttttatct tataaacacc aggctttact atatcgattt cgccgttatc
     4261 tctcataaca ccaagtgctt ttgcaaatgc tctgtatttg atatcttctt ttcccagaag
     4321 ttccttaaga gtaaatgtat ctccaacttt atatgtttca ctgatatact ctcgtatcac
     4381 agattttgcc gttttctgct tcagcttttc cgaagagata ttcaatctat aaatacctat
     4441 tcttcttgaa acaagatatc ctctcttttt cagaatcata aagattatct tcagctgttc
     4501 aatatcaata ttttcaatct gctgctttgt gatgtcgata tcctcattct cctgataatg
     4561 agtaaaaaga tagactgcaa cttttgcatg catagaatca tagccatcca gactcacttc
     4621 gttatacttc gtcactggca gtgcgtttgt tgatacgcca cttcccaggc ttttcagaag
     4681 gttcttctta tcaatattga atacttcacg cgtattcata tccaataatt ctgcttcatc
     4741 attatgaaag cctaataagc tgtattgtct ggaagttctt tcctccacga aacgcagcag
     4801 ccatttatcc tgccgtattt tgcataccgg acatctgggc ttcttgtaaa acgcatggat
     4861 gcgttcacta aattcatgcc cgcaagtgtc atgtgtaatc actacatcct caatgccgtt
     4921 tgtataggaa gaaagcgtaa acggaccata gcttcgcacc ttagctgcaa cctcatcaaa
     4981 cggagttgtc tcgcaggtac cgtttttgat atcgtccgga accatcttca gtacctgata
     5041 cggagtattc acaaagcttt ctctcctcct gtaatcgaag aattccattt cctccttcca
     5101 cccggtaagt tttgcatttg ttgtaaacct gccgtttgat gaaatctgga tcatcttatc
     5161 aaaaattttc ccttcatcct tttccatgca acagggacat gaccatccag attctatccc
     5221 gtatgcagtt gcagcaaatg ttgttccaca cgctttatgc ttcagttcaa gaatcacgcc
     5281 tcttttatcg acaaccgtat atccttctgg aacgatatac ttcccctgct tttctactgc
     5341 tttccggaaa gattcaacgt ctccgaacag agcgtatgca caacacataa gaatattgaa
     5401 acgttctccc agaatgatat tcctcatcat tgtagattct ttatacatgc gagaagctaa
     5461 tgcctctgga ttttcaaaac caagtttcgt cataatacaa agatatctat cctcaagatc
     5521 aggctcttct ttcgcttttg cacctaatgc ctgtcttaca gtataaagat ccgtgctgat
     5581 attagccttt agcagttcat ccgcgaactt cgcatatttg acctgctcct ctaaatcaaa
     5641 acctgtaacc ggttcgaacc gttcagccat tcctttcctg tctttaatta ttttcataag
     5701 cggcattctg tgtttatgac atatttttac accatacaga tgatgtccac gccttaaata
     5761 atgtccctct tttagacaat ccggacacac atagatatca tcaatatatc ctgccataat
     5821 atgtcctaca atgtttggat agtcacgttt cttacgtacc atcgcattaa cagtcaggta
     5881 ctgcttcacc ttactcataa atagcaaata tgctggcaac actgtgtggt cagcaataat
     5941 ttctgcaccg gtataatatg atgataatcg cggaagtaga aaactgatat cttctaagga
     6001 gtccaatctg agtttggtat cgtccttatc agggaaaatc cctctatgat tgaaaaaggt
     6061 ttttgggttt gtaaatccgt ttgcctcagc caggcggagc aaatatgaat acaacaattc
     6121 ttctttatac atctttggaa tgttaagcaa tctcatttta ataacctcgc attttttaaa
     6181 tacaagtagt atatgtcaga aaattgacac ttaccaagct cttaggattc acgattctga
     6241 tatcaacatt cttcatttgc tcaatacgca taaaaataga cctccagaat tttgtgcaga
     6301 aaaaaggcac cgacgtttcc atcgatgcca taataatttg ctattgtaaa aattaccttg
     6361 gaggaatcaa ttcggcaatc attttagtcc atagtgtttt taactcgtct tctgatatga
     6421 ttctaaacac tttttcatga gtaaagtaat ctaattttcg gaatcgtatt tcgcaacact
     6481 ctgttatgga aattccctta gtgtaaacgc ctcgttcaag ttgttccatg ccataattat
     6541 tagcaaattc tttgtctcca gacataattg ctacaatatc attttcaata gaatatgaag
     6601 caatcccaca ccactggcca tgccatttca ctgaaaatcc cggttcaaat atctcatcga
     6661 cttcagagat gtcaacattt ttttcgtaat atacatttcc ttttctagtt attccctttt
     6721 caaagccatt taatggacga tatgcccaaa tgtttatagg tgtttgagac atatctatat
     6781 tacaaaaata atttttatat ctagcaatca taaaattctc tcctctaaac caaagcaaaa
     6841 catttatctt ttttatccaa ctccgctaca gcagtttctg ttccatctgg ggaaaccctg
     6901 taaatttttc catctgttat aatagcaccg ttaccatttt catccttttt tactgtatac
     6961 tcaggaacta gatgcttgct acttcctgta tacccattaa gagtacatgg tgctccttct
     7021 ttacagttgc cgccaaattg ttcaccatat ggaatttcat agttagttcc atctgtataa
     7081 cgcaacacat aaaccggttg ttttggatcc ttatatggtg ttccagggta atcagttctt
     7141 aaattgacat agcattcttc aggagtacgg gtaaacggag tgctgtcttc agccttagca
     7201 acaaatccaa atacctgtgc gccagttttt tcaccggtta cattattttt tggatcaaga
     7261 taccccttca aatcttcttc tatcttcccc gtgtcaaccc ctatgatttt ttgcattaca
     7321 gttttttcat ccggatgttt gacttcatcg cgttcaacct ttaaccttgc tgcttcttct
     7381 ttggaataaa tctcacttgc ttctggatgg ttgtttgtat cttcgcccat tggaactaac
     7441 tctgaattgc cttttgcagc aagttctctt gttccctttg atgcgcgtac atcataagat
     7501 ttttcaactg tgaagttttc tgaatcgaat ttactttgga tttcattaag atttacagtg
     7561 taagtctcat ctttcttaaa agaagcatca gaattctgct ccgcttcatt accctgaata
     7621 tcacggattt ctgcttcata tgcctcttta gcatcaacag cctgatttac atcgttaata
     7681 ctgaaaccat tgttttctac ctcattataa accggattgt ttatagtttc aaactgttca
     7741 gaagtttcgt cttcaattat actgttactg tccaagtgta atgatatgtc atcctgagct
     7801 tttgtatctt cgatactgtc tgtttcattt aattctgcat cattatctag tgtatctaaa
     7861 tcatctaaag ctttgcctag ttccttgtgg gcccgttctg tctgttcttt attagttttc
     7921 cggagctcct gattctgtac ttctatctct cgcgtattgt tttctagtcc catattatgt
     7981 tcctcgtata agagttatct ttataagcgg acaactcttt ctatgaaaaa atccgagaaa
     8041 aacattatat aatgatttcc ccagattatt ttctgtaaat atataatcag tatttagtcg
     8101 atcacaacgt ccatttttct tgttttacca tctttcaccg ttgtaagaac tgtttcttcc
     8161 cagaaaggaa gaatatcctg tctgaaatca tccatcacta aatccttaaa ttcaagatca
     8221 tcccttaaaa aatgttcaaa gttatcaatc acgatgcaga ttcctctgtc tgaatctaac
     8281 caggacaaat ccatcatcca gtcatcaaaa cggcttactt tgtttttgca tgatgttggg
     8341 aaactgaaca aaatctcaac agcatcaaaa aactcttttt tggtagatac tttattccca
     8401 tctattgtga aaatatagta attttcagca atatagtttg tatttacatc ttctttattg
     8461 ataatagaat tactcattct gttcctcctc atcatcaatc acacggaaac tttcgtagtg
     8521 atcatttgtg taatagacat tttcgttgtt atctcttaca aaacgctctg catctctatt
     8581 agagcctggt tctttatcgt taacatcgta ctctttatat gtaactggct ctccgttttc
     8641 atcgttctcc ggaagatctc catctttgtt tttccattct aagccaccgc gtgtacctgg
     8701 agtagcatct tcgtgcggtc ctgtccacgc atttgactcg tatttgcgat aggattcctg
     8761 aacgttatct ggaagttctg aaagtttaac ttcttgaatg tttgtttcat ctactgttga
     8821 tacatccgaa agcgaatctt ctatctcatc attacctggg atttcttctg tagacctttc
     8881 catatcaaca gcatcctgtt ctggttcatt taatcgagaa cgattgtcgg tttctttgtt
     8941 cgatacatct ccttgatgca ggtcattacc caataccgaa ggctctgtca tattcggctc
     9001 actgccattc tcctcaacac gctggtcgag ttccttctgg tcatgatatt catcctgact
     9061 catatcacgg atttccgctt catatgcttc tttagcatca acagcctgat ttacatcgtt
     9121 aatactgaaa ccattgtttt ctacctcatt ataaaccggg ttgtttatag tttcaaactg
     9181 ttcagaagtt tcgtcttcaa ttttatcatt attatccagt tgtgatgctg catcatcctg
     9241 ttcacttata tcttcatttc cgcttatttc atcaagctcc gcctcattat ccagtgtgtc
     9301 taacccatct aaaactttgc caagctcttc gtgttccttg tttgcctgtt ctatctgttc
     9361 tctattggca cgctggagtt cctggttttg tacttctatc tcatgcgtat tattttctat
     9421 tcccatgtca tattcctcgt ataattactc gtttccatca ttttctatcg gcataccaca
     9481 ttctgtacag aatttgcttc cttccataat ttcagcgcca caattagtgc agaatctttg
     9541 tttcggcttc tgagaagatt cctttgcttc attttcttcg cataagtctt ttccgaagtt
     9601 ttgattaatt acttcagtag tttctgtaac tttatcagat gcaactttgc ttgcatttga
     9661 taatggttca aactgagcaa aatctacaga tgcaatcttt ttacattcct tgactaaaag
     9721 cacggcggca ataaccatta tcaacatcga gaagaaatag attgtcatat aagaactttg
     9781 ttgtatgatc aatcctataa attcagatga taataaagtt aaataaatag cagacaacaa
     9841 cacattaaga tttagcaagt gcatgtttgg acgttgtttc cgagtgttgt ggtaatgaat
     9901 tattaaaatc gcaataagaa taaccaatag gaaaaacaca attggaatcc caaataggct
     9961 cgctgtcctt tctgtagttt caaaaaggcc ttcataacga agggtatatt taaacccttt
    10021 agctaattta agtaaagata tgttagtttt agcatattct ggcggaccag atatactaaa
    10081 aaacgagtcg cttacctttc cctcagaact acatacaggc aaaacaataa atgcaactat
    10141 taaaaaaaga gataagataa taactggttt tttgcctttt tcatataaat caatatctgc
    10201 ttttttcgtt ttgctgttcc atatgataac accgtttttt tctgcaatag aaaagtttaa
    10261 aatagtacaa taaatcaaaa tagctattac aacaacagta ataatagttg tatttttctg
    10321 taccgcaaga atatattgat aaaagcccat gtaaacaaga caaataatca cgttgttttt
    10381 tgcatattgg cctgaatttt cttgcttttc taatacgtga tttatgtaca ataagagtgg
    10441 agaaataaaa acaaaaagat acaaaatcaa caaaacaaaa aataataccc atttcaaaat
    10501 tcctggcaaa ggataattgg aaggcaagat accgcccatt gcagttacat gcatatggaa
    10561 agctttccca tcatttacat aaactgggag aaaccacgaa atccctacta ttagataaag
    10621 aatagtctgt ttatcaatgt tttttcgtgg gtcaaacatt tttcgattgt tacttataaa
    10681 tttagcagtt tcttttgctt cccctataat tctactaatc atattctcat tttcctttca
    10741 ttttatcagt cagatatttg cgctaaattt ctatagcagc atatcttcca tgatattcat
    10801 ttaatttata aacagcttat tataatctaa aacggtttat tcattttttc tatgagcttg
    10861 cgttctacag ctacataatc aatcccgttc tgctccacat tctgagcatg aaccagatat
    10921 ggaataattg aattattgta tgccggagga agtgaaatat atttttcaag ttcttttcta
    10981 aatctattct tccactcttt tatctcatac tcatcaggct gatcattttt ctttattttt
    11041 gattccagag tttttattag atttttcgtg caggaaatgt ttaaaagaga agttgtaaaa
    11101 aattcaatat ctctcccttt ttcgagacgg gacagcgtat atttcataca gtacttcatt
    11161 tcttttttct tcaatgaacc aatctcagta ttttcaataa cgtcatacat atgctcaagg
    11221 ctgatttctt tatctctaat gtactggttc atcagttctt caattacgtt tcctcgaagt
    11281 atccttaatt catcaaataa taccggattt atctttttct cgtatttgtt tgagtatgca
    11341 tcaatatgag taagaactgc ttcaagccag ttgttctgca ttacaactgc aattcctgtt
    11401 gaaagtttgg acatttcagc aatctgctta tcgttaagac cgacagcatt cccggctgtc
    11461 tggcagtctg tcttttctgg aagacgcata agaatctttg tatttgtatt ttttattgca
    11521 gcaatatcta ctgctgttgg agactggtca acgattacaa atccttcccc atacgtacgc
    11581 atttctgcaa tactgttgca gatcatttca acagatttct tcacaacatt tccacctgct
    11641 gcagaggaat cgactttttt cagaagatta tgggcttctt caagcacggt aatgtgtcta
    11701 agttttgaat tcgactcatt tgcatttgcc attcgatatt ctgaaagctt caggacaaga
    11761 attcccatga tcaatgactt tgtttccgta gaaccaacac ggcttaaatc tactattgta
    11821 ttttcatcaa aaaatgtgct gtccggaata tcataggaat cacagaaaat ctggcctgat
    11881 ataccattag tcagtgatgt tactctggtt accagagcgc cagtataatc ccccttggta
    11941 tcagcagaat aactggattc attgattact ttaggtaaaa cctgcattaa atcactgaat
    12001 gttggatata caggctctcc ttcccctaaa taaatggaat ttaaaagatc ccaacctttt
    12061 tcgatgtagc ttttttctac cgcgtctttt aaaatagcag gcatagcagc atacatttcc
    12121 cagcacgcat taaaaatttc tatcagtctg tccaggtgtt caagaacatg aatccccgga
    12181 tcaaaacgga aagggtttaa tttaagcatc tgtcctatca gagagtttgt tgtaaaaatg
    12241 ttgatgtttt cgactgcgcc aaaaacgtct ttatattcac cttttgccgg ttcaacaatt
    12301 aaaaatggaa tatcgttttc catgaatttt tccagcagtc cataaaccgt atttgattta
    12361 cctgaccccg tagaaccggt aataaaacaa tgcgatgaaa aactgtccaa atctaatttg
    12421 acctctgttt tttctgtctc tcccatatga tgtatgcttc caattctgat tttctttgat
    12481 tctttcgttc tgctttgtac aataacattt cttccaaacg aagctacaga actaattgca
    12541 agtcctgcta ttgacttgtg tggaactccc aaaaataaag gcagttcttt accactgata
    12601 taatttccag gagtcacata ctgtctggca tacagatttc ccattccctg tggcggttct
    12661 atttctatga ctggatgtct gccgcatgtt aagtattcca gcaccttctt tgtattacct
    12721 gatttatttt ccgaactcca gatattaaag tatgagtttt caacatccgt atcatctcca
    12781 atcatcagcg ccttgaacgt acttgccgcc attgcagatg tttctatgct ttctgccacg
    12841 aaatatccag cacattccca gattccgaat gcttcacagg atttgattct ctccaactgc
    12901 tcatcgatct tgtcaagcat attggttatt gctttatttt cctgctcgat tgtcattgtg
    12961 tttgaagaac cggttgaagc tgtatcagca taagaggatc catgccctgc agtatccgta
    13021 attcctttgg aagttgtttt cgaaaacgat gggccaccgc tcaatccaag actggatcca
    13081 cttgaactgc caccattaag accaaaacca aaaaggttta gtcctccact tgtgttggaa
    13141 ccactattca taccaaaatt accattggat gagaacccaa tggtttttga tacactttca
    13201 ttgactgatg ttgaaaaatt atcaaacacg ctttttgaaa cagaagaatt ctcattctct
    13261 ccatatgcaa gtgatacctt tgcaagcggc gaaagactgg aatatagttc ttcaagccct
    13321 tttttacgtt cttcaagttg ttccttattt accggttctg cgataaataa cgcattatat
    13381 atctgcccct gcatggtatc aatgtacttt tcaaggccct gaacaaattt ttccttgtct
    13441 tcgtctcttg aagctggcac gactgttatg ctggaaacat tcttgcacag atcgttattt
    13501 tccgtacgac cgatattgtc tattattctt cgtatttcag atgctttgtt gattcgagta
    13561 agtgtacttc ccggaaaatt gccaataaaa cttttttcaa gaaccttatc ggctatagtt
    13621 atattatttt tctcttttac tccaacgtaa aaggacgttg aattagctgt tccttcaaca
    13681 agcagaaaaa cagtgctgtt gatttcctgc agagcgctat acacagatat cagcttatca
    13741 ttgatatttt cgttttcatc atagataagt ttgtccagtc taaaaagtcg aatcagacgt
    13801 ttttcttctt cataatcggg aagttccatc acttctgaat cggacaaatc gattagatat
    13861 tttttgttta taaacttgtc tgctatggaa agtccgattc ttaacataag atctcgttct
    13921 tctttaatag cgactaaatc aattttatta tccatatctt tccctcttac taaatagtca
    13981 tacttacaat cacgaaaaga accgcaagta tggccagtac agtatatgta tttttgcatt
    14041 tattttcttc caggtgtttt aatgctaatg cctttaacat gttataggcg caaataccag
    14101 ctactataaa aaacacgtat ttaaaaggat tcattaaaac tgtaataaat cctgatatta
    14161 cagataccac aaatgctaaa agcccttttc tgtaatattc catgctgtca gaagtccttt
    14221 ttgaaaatgt actggcaact tcaactatgc atttgccgac aactcctgtt tttacagcct
    14281 taaattcaaa tgtgcaggaa ccaactttat ttgcagttat tacaaaagta ccgtcgcttt
    14341 ttgcttctag tgatgcgaca tcggaatctg aacacgtata tgtgattcga gaatcagata
    14401 cattgcgtgg agaaattatg atgctgatcg atttcttttc atttacatcc aattcaagcc
    14461 tatgcggctt tatagttatg ctttctatgc agatatccga tatatgtacc ggtttactat
    14521 caaatatcgt tttttcacca tttatcactg tataaaaaat aacattcgct gtacccgcgc
    14581 tctttggagt caatacatta tttctgattt ctagtatagc tgtattgtct gaatccattt
    14641 gaaatgggac atctgcagtt ttatctggat acaaatagac ttctggtttg tatgtatcac
    14701 gtattttaag ttctgggata tctgctgcat aataaaccgg ttcaactgaa atgcatgttg
    14761 taaataaaag ctgatcctgt ttatcaagta tcgagtaagc tgaactaatt ttatctgcaa
    14821 ttgttctaat ttgtttgatg acactgaagt aatcaacaat gctgttaaat atttcagttt
    14881 ttcgtattgc tggcacattc cagatatatt gctttttacc gtcaatattt ttcccgatac
    14941 attcagcctt attgctttcc gacgtactga gaataatacg cgcagtgctt tttctgtatg
    15001 cagccagagc agaaatatca tcgtcaacaa tacaaaaagc tttttcagga gtatcgaccg
    15061 ctacatacgc agcattactt tcgacaaagt ccttgttttt gcagaacact tgcagcttat
    15121 aaaacggttc tgtactgaaa ttatattttt ctgctaagcc ctgaagagat cttaacctat
    15181 cattagcatc tatatgatta ttatggtttt ctttccttaa tgaaaggcat ttataagcgg
    15241 catcttcgga tgcataaaca gatattatgc cttccaaaaa ttccttttca aaatattccc
    15301 cacaaactga cagattgaaa tcatcatata ttgctgtccc tattttttca gatatctcat
    15361 ccgcccaatc caagagcgat gaattttcat aatcctgaaa atctgtcttt cccgtcatct
    15421 tacttccatt acagaagaat gtaaatttct ccttgaatgg atctgattgt atttttgcct
    15481 gttccatcac ttaacgcttc ctccttctat ctgttctatt ttttattcga ttctattgcc
    15541 acaatatggg caaaatttcg ccccggcagg aattacttta ttacaagatg cacaagtctt
    15601 ttttgcatcc tgtgttttaa gagactcatt cactattgca gtaacaggtt cttctttgtt
    15661 gtcggctgta tcatcatagc tgtcttctgc tgcatttgtc acattaataa aaactggcgt
    15721 ttgttcgttg ttttcttcat cttctgaatc agatgaatta tcattttcat ccatgctctt
    15781 cattttataa tcgcatccca ttttctgtaa attgccaatt cgagcgtcaa tttcttttct
    15841 tggtcccaaa agtgtatcca ggtattcaca tagctcattt ccatacccaa tattaaagca
    15901 gaattcatcg gataaagatt cgtttttaaa tacttttcca tttagaaagt atacaagttt
    15961 tccaaaacca attcccagaa cggctttttg agcatttaca ggaagtgtaa agaatgtatc
    16021 tgttattccc agcgtatttt catttaacgc cctgataata tctacgggtt ttttattgct
    16081 caggttaacc ttcttttcga tattctgagt atcggtatat ttaacataaa cccgaacacc
    16141 ttccggaatc gttgaatcga ctcttcttgc tgcttcttca acttctttta acagcggttt
    16201 taatacgcga agatgctctt ctcttgttat ttcctgatat ccaaatgtca tacgctgacc
    16261 aagattggat atcgtttctg ttattccact caaattgact tgttcaagcc ttgataatcc
    16321 aatagttttc agtttttcat agtctacttc ttttaatttg ccaaggcttg cttcttttat
    16381 cttgtttaaa tcaacatcct tcaacttacc caggtttacc tctttcactt tattaatatt
    16441 aacttccttc aatctgctta agtctatatt attgtctttt gcttttgcgt caattttttc
    16501 tcctgcttct ttaactacgt ccgatatgtc gtctaataaa tctttccagt ccatttgcat
    16561 attctcctaa tcatattctc ctaatattaa aaagttataa ctattttgta caattatatt
    16621 ttgtattggt ttcactacct aaccctcatt gtagcacaag taaaacagca gttaaaagaa
    16681 aaaacagttc tctttcagat tttcatcacg caaaaaggac acgcctatca cagacgtgtc
    16741 tttcactaaa ttttctttct aatatagcag ctcagttcat aaaacgtcga acttttttcc
    16801 aaaagctttc acactataaa atacgcaaca ataattcgaa gttgcaaatc cgtcaagtcc
    16861 tataaacttt ggcaaattcg agattaaagc ccttaggctg tatgagaaaa taaaattcac
    16921 tgatttttct gacatattat aggtacaaca ccattcaggg ataattttat ccctatattt
    16981 agggacaatt tgcaacatat aacgacaaaa agcagtccta agatatcctg tgagttagtc
    17041 tttttcagac ttgtactctc ggacgatacg ttgaaacatc ttttcaccga cagaacgtct
    17101 gtcaggagtt ttatttgtaa gttttgctct ggcgttgtta aacgaaatca gcccattcac
    17161 ataatcctga accacatttc ggccatctcg agtgagtgtc cagtagtgta cgttccgtga
    17221 aggtctgccg ggttttcttt tttcagagtt aggtttttgc gtattacggc gtttcctgtg
    17281 agcggatatc tgatttaaaa cgctgttaag taaagcataa tcagagtgtt cattattgtc
    17341 ctgatagttt atcgacgtaa tccgcagatc taccccactg gagtggcacg tacggataac
    17401 cccctgtatt gctggaattt cactagcaag cacgtacaga tgggatacat acacaatatc
    17461 ccctttttta ataggtctat ctgcggaagg tttatgtagc cgttcttccg tatcggaata
    17521 tataagctcg gtcagatctc tactaaatct aaatcctggt attgcttgag gttcgccacc
    17581 aagccagtgg cgccggtcag gcacaagttg ttcggcaatt ttgcattgct catctattct
    17641 tgcgtctttt gtatctcgga aaaaccacca tttttccata ggcgaacctc cttccacgtg
    17701 tttttgtagt ggttagtagt agtaaccact acattataac atgtaatgag agagcgcagt
    17761 taataaccaa ataatatcaa acgcaataat tgtatgtttt tttattataa attatttata
    17821 tgtaagcaag caggtttttg tgcattttca gaacgcatta attgtaaaaa acaggtgcaa
    17881 agtgccagga ttttacgcgt gaagaagcaa attaccattc tgattatgac atatacttga
    17941 tgagtacaga aaaccaagaa aggagtatat atgcttattt caaacagatt tccacctctc
    18001 gacgatgaat tatctatttc atatgttatg aggcttgcat ttgcaaatgg atttccaaat
    18061 gaaactgatt ttcttagggc ttttgtctct actgacaacg gtctgcagca tacaaatgac
    18121 ttttgtctgg acactaaggc tctggctgac aaaatcaata ttatggatcc tatcgaacaa
    18181 ttagcgcaga caactgttat ccctgcactc tggccgctgc tgcctgcatc catgcaggac
    18241 cggcatatca gaaaagcatt taacggatgt aaacgttttg gcagtgacaa agtaaatctt
    18301 ttaactccac agcttcatta ctgtcccgaa tgtatgaaac aggacaatta ccttcaccgc
    18361 atacataatg taccgggaat ctattactgt ccctttcaca aaaccatatt aagaaacttt
    18421 catggtaact ctgaggttat cccactagtt tcatacgaag ctcaggcagc ttatcaggag
    18481 ctctttattg ctcttcttaa aaagtggata aaggctgact acagaacctc caagcaggca
    18541 cttgtaaaac gcgtcagaga tatgacatct gttccgattt atgaatacct tagtgatatt
    18601 ctctcagaat acggtattga tgattattcc gctgcaaggc tttcttcgtc acttaacagt
    18661 tatatgcctg gggttcccat gcacattctc ataatgctgt tcgggacagt ctttcctagt
    18721 gcagacctcc tgatggatta tctcgcaaaa gtctcagagt tatacgattt agaagaggtc
    18781 tcccctgaag aatacgatct tcttaacagg acttcccttg gagtaatcca cttgaggcac
    18841 aaagcatgcg gtcattcatt ctacaccaca gaaaacagca tccgggaagg ttggctatgt
    18901 ccgaagtgtg acgcacgatt atgtgaccag gatttctttg aacagattgt tgcacacaaa
    18961 ggacgtggtg aatatcgcat caaaaaagga tatcgtggtc tacgttataa ggttgcatta
    19021 gaacactcct gcggacagtt gcttgaagta tcggcttcaa aatatctcta ttacggagcg
    19081 aaatgtgcgt gtacacacac attattgctt tcagatgctg ataaacaggt tcggaaagca
    19141 ggcaatttca ggttactgtc tttttctgga accgataaag atgccactat tcttagtgaa
    19201 gactgtggac atacatttgt ttttcattac acaaaattca tccagcatcc ctgctgcaaa
    19261 gcctgctctc cgcgctgtcc tgatgaagac atgttccgca aagaactcta tgacctggtt
    19321 ggaaacgagt atgagcttgt tggcagctat accacccgtc tggcggaaac aatgttttta
    19381 cacaagacat gtggaagaac tttttcgatg cggccaagcc atatcttaac cggtcataga
    19441 tgcccctgct gcatccgaca gtatcgtaag tctgaatttg tagaatttgt tgatgcttat
    19501 acctgcggaa ggtatgaagt aaaaagcttt gttggaaaat ccaaggcact gattcttgat
    19561 aaatcatccg ggcgggaaat gcttgatgag agatgtttta tcatccagga gcttacccgt
    19621 cctaccccat cgagcagata tcctcatgaa aaaccgtctg ataagcctct gttgcttgca
    19681 tctgacaggg tattagctgc tatacaggaa ctctaccctg atccagacag tatgatcacc
    19741 accagaggaa tacaggtttc tgggttatcc acaaaacaga ttggccgaat catggcaaat
    19801 cagctgacta agcttaatgt tctggaacgc atcggccccg gaacctacaa atacaaagag
    19861 aggtaaatat aatgaacaga atcagtaata aaacaaaaga aaatcgtggc tacgggaaag
    19921 gtgaaggtaa aaactataag ccttatatcc aggcccacga gtttaactca cagggaactg
    19981 ctgtaactct tcgcgactgg attaccggtc gctcggtaga acttctctct cagggagaag
    20041 ctgaatactg gcatattctt agatttaacg atgatattat cgacattcgt gaacagattc
    20101 ctcttccaaa agaagacaca acagttattg ctgaagaaat gggctgtgag cctgccggca
    20161 agggacgtac aactatgaca tctgacttct ttatcgtgta tgcagatcag catatggaag
    20221 ttcactcttt caagaaaaac atgcgcagta ttgaacgtca gcgtacatta gaacttttaa
    20281 acatcgagaa aactttctgg gaaagaaaga acgtttcttt tcgaatggta ttcggtacag
    20341 acatcgatca tattcttgcc agcaatattc gcgtgtgcag ctactattac gaagaaagtt
    20401 cagttttcga tgagacatcg tgcctgaagc acctgatcat cagaaagaaa atcgatgtag
    20461 accttacaaa agaaaaaatt ggtattcctg tattgaggga tgtgtacaga aaggagattg
    20521 ccgcatggat gaaatccaga gaaatgtaaa taaaggtttg tgcagaggtt ctatcctcaa
    20581 tgtaagatct gccgacaggc tcaaagctgg cagatacaga gtccttaata tcaggaatgg
    20641 acttgttact ctctgtgaga tggatgtatc ttcttttaag ctcaatgtac aggttttaac
    20701 gtatcttcag gatatgcttt acgatggcat gattgaaatt gagtctgaag aaaaactcgt
    20761 ttttgattat gagaagcttt ctgaatatca gaaaaatgtt tacacccgta atctttccat
    20821 cgttaatgct gtcgcagatg cgtatggacc ggattatcta gacctagcaa cgcataaacc
    20881 gaagccgtta ttaaaaaagt ttgaagatga gtatggtttg tcaaacttta ctgtccgacg
    20941 aattattcgt ctttatattc aaagtggatt taataattct tcgcttcttg atcaagctca
    21001 attcatgcag aaaaaacagg ataactattc ttgctataca aagaaaaccg gaaggaaatg
    21061 tgaaaatgtt ataacaggaa aatgtcttac ctctgaagac attgctcatt ttgcagaagc
    21121 tttggaaaaa tataaaaaag gacgtaaaaa gacgtttaaa tctatttttc ttctgatgag
    21181 tgataaatat tattcgaaag aaattacctg tgatgatggt acacagctta tcgaactgtt
    21241 accagcttct gagcgccctt ccctccgtca gtttacatac tattgtagca aacatctttc
    21301 aaaggaggaa aaggaccgta tcaaaacaag cgccatggaa cagcgaaaca ataagcggct
    21361 tctgctttca gactccagat ataacgtcga aggccctgcc gaccgcgttc aaatggatga
    21421 ggttgaagtt gatatgtcta ttgtatcatc attagacccc tcaataaccg tcggtcggcc
    21481 aatcgtatat gttatgtatg atgtgttcgg ttcgaatatt ctcgcaatag gaatcagctt
    21541 tgacaacaac tccgttctcg gaatgacgaa ctgtttcctt aatcttgccc aaaacaaagt
    21601 agaatactgt gccagatacg gaattaccat ttcagaagat atgtggccgt cctgctacct
    21661 tccaggtaga atccttgtag atcgtggtgc tgaataccgc agttatgaag ctaaacgaat
    21721 ctttcgtgag cttaacatcc accgcgaact ggcccccgca ggttctggtt ctatgaaggg
    21781 tctagtagaa caatggtttc accagatgca ttcctcacag aatccttctc ttgaaaagca
    21841 tggactgatt gagaaaagac atgattccaa acatcataag gaagcctgtc tgacaatcga
    21901 tgattttaca gtcatgctaa tcaactttgt gattcatcat aactgttcgg tgctctctga
    21961 ttatccatta actgctgaca tgatctctaa aagagtagac ccggttccag tggagatttt
    22021 taaatatggt tgcaaaaaat atggaacccc aaaaccgatt tccaatattc cacagtatta
    22081 tttcagtctc atgcttccac agagtgcaaa atacgatcgc cgtggaatcc gctgcaacga
    22141 cctattctat atcaacaaaa atgatcatga acttcttgct gacatgtata acgcacacac
    22201 taaacgtatc gagttccctg tgcgtattga tccacgtgat gtaggctctg tatactatat
    22261 cagaaataat cgtatcatga gagcatcatt gaattctgat aagacgggaa atgcagatgt
    22321 tgcaggaatg acgttaagcg aatggacatt gattatcaaa tctcttcgag caagacgccg
    22381 aagccttgag atcaataagc agcagtttga tacttatatc gaaaaagtaa acgaactgat
    22441 tgttaaaaat gcaaagaagg ttgtgactaa tcagccaaca acccagaatc ttttagaaaa
    22501 ccgggcaaaa gaaaaacatc tcgaatcaaa taatcttcga atgtttgact atcttaacca
    22561 gtatcttgat gagcagtttg gggaaactgt atctgcatcg atgttagaag cagaaatgcc
    22621 taatacttac gaagtgcttg attgtgatat agatggcgag aacaccaata tcggtggatt
    22681 gtcttcagat gcttctgaaa tgacagaaac tcgggaaaat tctaacgact ctgacatcaa
    22741 tattgccgaa aacccagccg cataccttgc aaaacttctt gctgaaggct ggaccccagg
    22801 aggtgacaaa taaaaatgaa gtgttttaat gatacatatg aatacgcaga tgacgtagat
    22861 cctaaaacca atcaatatgg cattgtggat ttcagcaaga ttgagtatgt tgaagcacga
    22921 tacgttaaac attttgattc ggagaataat ggcaatccct gtatcgaagc actgcccaga
    22981 cctagaagcc ttgaagaatg taccctggat tatttcaagc ctatccttgg atacagtatg
    23041 tcggaaatac aggcgcttcc ttccggggag cgtatataca gactcaatca gctccgtaaa
    23101 ctcaggcttc ctatagcgtt tgactatatg ctggaagtgg aaatgcacaa cgcattgatt
    23161 cgttcctatg aaaacagata tcagttttac gacccagaaa tcccggtatg gatggccgtt
    23221 gaccgaagca acggagtcaa gttcccggag caggctaaaa atgatacttg tggaaaatat
    23281 attaccaaca agacctccag cgtcagcggc tggtcccttc tcggtggatc cggaacagga
    23341 aaaagttctg ccatagatat cctgaaatct cactatccac aggtaataga gcatcctctc
    23401 accaagggaa acctgaaaca gattgtattt ctgcatgttt catgtcctgc aaactcaaac
    23461 ttcaaggtcc tctattcgac cattggacgc gaaatagata atgcccttag ttacacgacc
    23521 agtgtatatc agaatctcct gagtaattca aaagatccta taagcacggt tgttcagcta
    23581 atacagaatt tctgtattgg aattatcatc tttgatgaga ttcaggaaat cgacttcgcc
    23641 ggtaccaaat caaatacctt tgcaagttta gttactatag gagaactttc aggtgtagca
    23701 atagctgtat ccggtctcga atctgcatac agcaaaatgt ttaagaaagt ctatacagca
    23761 agacgcgtcg ggaaggtcat ttcttctaat cgctactgtt ttgataaatc cagtctcaaa
    23821 acccttatga gcttgatttt ctcttatcag tggtttgata aaaaggtgtc actcactgat
    23881 gaaatcgttg accgttttga actgtataca ggtggccgta ttgatcagct gattacgctt
    23941 tattcataca tgaactacga gtatctgata tctccagaaa acaaacggcc agaaataaac
    24001 ggcaaatttg tggatctttg tattgaaaaa tactatgggg atatgaggag gcttcttgcc
    24061 gaagctacca tttatcataa gaaaaacggt gattttgacg ggcgtatgct tgaagctttt
    24121 gaaaaagcaa agcagaacct caatgacctt ctaggagacg atgaacaggc agatatggct
    24181 gagcatcttt cttcgcagga gactttacag gctatgaatt catttgccaa tacacaaagc
    24241 aatgttgttc aaaacgtgca gaaactgttc ccacagtttg agatatctca aatcatcgaa
    24301 acgctgaaaa ctgtttataa cgaacctggc agcagctgtc ttgatgattc ggtactttca
    24361 ggtcgtgtta ttcaagttct tcttggaaca acgaattctt ctttggaaga tacttctaag
    24421 aaacctaaaa tagcaaaaaa gaaacgtcct gatgcaaatc tgcttaaaca aaacttatta
    24481 aatgtatatc accagcaata aaaaaagagg ttctacactt agatgtgtag ttcctctttt
    24541 ttacgcttta ttgtaaatct cgtaatctta ctttgaagta aaacgagatt tggagttgtt
    24601 taacgagatt tggagttgta tattaacagc tagaaaacta gctcttataa gtgtagtact
    24661 ctttctttga tttcttgtgt tcttccttgg ttccagaact gagttccaat atatccacaa
    24721 gtacgacgag ccacattcat tttttcttgg tcacgattac cacaatttgg acattcccaa
    24781 actaatttgt ggttctcatc ctctacaatt ttaatttcac ctgtatatcc acatacctgg
    24841 caataatccg attttgtatt caattccgca tacataatgt gattataaat atgtttcata
    24901 atcgccaata ctgcatcaat attattttcc atatttggaa cttctacata actaatagct
    24961 ccacctggag aaagtttctg gaattgcgat tcaaatgata atttcgtaaa cgcatcaatt
    25021 tcttccgtta catgcacatg ataactattt gtgatgtagt ttttatctgt tacatgagga
    25081 ataattccaa aacgttcttg tagatgtttc gagaatttaa atgtagttga ctccattgga
    25141 gttccatata atgagaaatc aatattttct tcagccttcc atttagcaca tgcatcattt
    25201 aaatattgca taacttttaa accaaatgaa gttcctggct cctcagtatg agggtgtcca
    25261 gtcatataat atacacattc gcataatccg gcatagccta atgaaattgt agaatatcca
    25321 ccaaacaata atttatcgat tggttcgcct tttttcaaac gagccaatgc cccattttgc
    25381 cataaaatag gcgctacatc actcggtgtt ccgagtaatc tttcatgacg gcacatcaat
    25441 gcttttttac ataaagctaa tctttcgtcc ataattctcc agaatgcttc ttcatcctgt
    25501 ttacttgaac aagccacatc cactaagttc aatgtaacaa caccttggtt gaaacgacca
    25561 taatattttg gttttccatt ttcatctaag tatgtagtta agaatgaacg acatcccata
    25621 catggataac aatgaccttc accattcgca tccactttca attccaacat tttcttttca
    25681 gaaatataat ctggaaccat acgtttagct gtacatcttg cagctaattc tgttaaatac
    25741 caatattttg aaccttcttc aatattgttt tcttctagaa cataaatcaa tttagggaat
    25801 gctggtgtga tccaaatacc cttttcattt ttaacaccct tgattctttg tttcaacatc
    25861 tcctcaataa tgatagccaa atcatcacga gtctgtcctt caggtacttc atttaaatac
    25921 ataaatacag ttacaaaagg agcttgtcca tttgtcgtca ttaatgtgat gacctgatat
    25981 tgaatcatct gtacaccttt ttcaatttct ttacgaacac gcatctctgc cattttattt
    26041 actgtatctt cagaagaatc tagatttaat gattccaatt cctcttttac ttcacgacgt
    26101 gctttttctc ttgaaacctg tacaaaagga gctaaatgag aaagtgtaat actttgtcct
    26161 ccatattgag aacttgcgac ttgtgcaata atttgtgtag ccacattaca tgcagttgag
    26221 aaactatgtg gtttttcaat catagtttca gagataactg ttccattttg aagcatatcc
    26281 tccaaattta ctaaacaaca attgtgcatg tgttgcgcaa aataatcgga atcatggaaa
    26341 tgaataattc cttgttcatg agcttccata acatcttgag gcaacaagaa acgttttgtg
    26401 atatcctttg acacttcacc cgccatataa tcacgctgaa cactattcac caatgggttt
    26461 ttatttgagt tttcctgttt cacttcttca ttattacgct caagtaaaga catgatttgt
    26521 ttatcggttg aatttgcctt acgaaccaat gcacgctcat aacgatacgt aatatagttg
    26581 tgagccaccg caaaacaacg atgctccatc aattcaccct caaccatatc ctgaatttct
    26641 tcaacatttg gacttctctt taaattttca catttatcaa taacactctg tgtcacagaa
    26701 cgaattaatt catcagacat ttgattatgt gtagttgctt tattggcttt tttgatcgca
    26761 ttttcaattt taatcgaatc aaaaacaact tcttctccac ttctttttat tacattcatt
    26821 ataattcctc ctaaaatagt tgaaccttca actattttta atgactatct taagtatact
    26881 tatatttttt tctaaaacaa gactttacat tcctacaaaa taatgcttaa gcgatacata
    26941 aagcaaaccg gtttctttat ttttttaaat caattaattt gtataaattt tttatttcct
    27001 tgactatttt gtgcaaccct cctttatcca taagatacct cttatacaga catcatttat
    27061 gtcagtttta tttttttcaa tatttcatgt taaattcaca tacacttgat acaatagagg
    27121 aaagaaaagg agatcatttt atggattaca aaatcaaagg ggattccgac tgccctatcg
    27181 ttgaaatcaa tttacagaat aatgaaacga taaaaatcga aagaggctca atggcctaca
    27241 tgtctaacgt tacaatcgaa ggtaaaatga acagcaacaa gaaaggtcta ggcggtgtat
    27301 taggagccat tggacgaagt cttacaagtg gtgaaagcat gtttatcaca gaagccactg
    27361 gtacaagtca tgatggttta cttggtattg caccttccat tccaggaaaa attgtatgtt
    27421 tagaagtcaa ttcacaaaat cattactact taaacaatgg agcttttcta gcctgcgata
    27481 atagcgtaag ctacgaaatg aaaacacaaa gtgtaggaaa agccttattt ggtggaactg
    27541 gcggattctt cgttatgcat acatttggtc aaggagacct tttaatcaat gcgtatggag
    27601 atatcatgga aatcaatgta gatgatgcac acccaatcac aatcgacaat gaacatgttg
    27661 tcgcatggga tgattcttta gattatgaaa tcaagattgc ttctggaaca tttggattta
    27721 ctactggtga aggtttagtc aatgtattcc acggcaacgg aaaagtatac attcaatctc
    27781 gtaaccttca ttcacttgcc gatgcattga ccccattcat tcctcaaaca aaagactaaa
    27841 aaagcgtgat tttcacgcct ttttatttac agccatcgcc tttacaatca atgcaagatc
    27901 gaacgcattt attgcccttt aattttttga agctttcttc actaattgta tgttccattc
    27961 gacaagcttc ttcttctgca agaatcggtt ctacacccgc atgcagcaat tgatttttaa
    28021 aataaacatg tctttcatac acttgagttg cctgtagttg accttgttct gtaaaaaaga
    28081 tatatcccgt tttatctgta taaatcaatt ctttttcttt caagtccttt aaagcatgtg
    28141 aaacactcgg tttagaaaaa cctaaagccg cagctacatc aatagcacga acatggtctt
    28201 gttgagactt taatacatat attgtttcta aataatcttc ctgagattgt gttagttgca
    28261 tttctatccc tccggttagc ttaaacttac cataaaagta taacaaaatt tcacataaga
    28321 ttcaaaactt ttgcacaaaa attaaagtta atatacactt ataacgtatt agaaaataaa
    28381 aggagaacaa aatatgttta aatgtaataa attattaggc gcatcccttg cagttgtaat
    28441 gggtgtttca gcttgtgcca caaatgtatt cgcaatcgat acatctgtca ttgatgaaaa
    28501 gtggggtaaa ccaaccgttg tatatggtgg tggtttaacg aatgatcaaa ttcaaagtac
    28561 acgtgatcta ttcgatatcg aagatacaaa caacgtatat gaaacaagtg ttgatgccaa
    28621 tgatctatat aactaccttg gaattgcagg tggcgataca tctagcttga tttcaagtgt
    28681 tatggtacaa aaacaagatg ctggaaaagg cgtcaaagtt aaaatcatca cagaagataa
    28741 catcacaaaa atcacttcaa accaatatgc caatgccgcc atcacagctg gtgttagcga
    28801 tgttgaaatt gatgtagctt ctgtttccaa agtaacgggt gaatctgcat taactggtgt
    28861 ttataaagct ttagaagcca atggcgaaac attagattta gaccgtactc aagttgccca
    28921 agatgaactt gaaacgacaa atgaaattgc cgaaaataat gcagataatt caaattttga
    28981 ttctggacgc ttagatcaag ctatggttga aatcaaacaa gaacttgccg aaatcaaaaa
    29041 aaatcaagga agtgctgcaa cagctgaaca agtcgaacaa gttgtaacag acgctttaaa
    29101 gaaatttaat ttaagtgaca tcatctcaca agatgatatt gataaattaa ttgcgtttgc
    29161 caagaaatac caaaatactt cagccattga ttcacaagaa gtattggaac aattaaacaa
    29221 attgtctggt aatttaaaaa atcaattatc tggacttatc aacaaagcac aatctgaagg
    29281 ttggttcgat aaaatcatca actttatcaa aagtctgttt aactaaaaca aggaagtctt
    29341 aagcgacttc cttttcattt gccatacgat aaatttcaag catatctgtt tcatccaatt
    29401 ctttaaattt tccgattgta cgcgtatgct caaaactaca cttatatgtt aattcataca
    29461 attcttgttc cgtacattta cgttttaata attcctgaat acttgtaggc atgccaatca
    29521 ttttaaagaa ctcttccatc ttttcaattg tattcaaagc acaagacaaa gtatctgaat
    29581 catccaagcc aaacacacga tgtccaagtt tggcaaatcg cgttggattt tccatatata
    29641 catggtttgc ccaactatcc caaattgcag caagccctgc tccatgtgcc acattaaaca
    29701 tgccactcaa ttcatgttcc aattgatgac aagcccaatc ccctaaagaa cgatctcctg
    29761 tcatatcgtt atgcgataca ctaccagccc acataatttc agctcgagct ttttcatttg
    29821 taggatcttc caaaagaatc tttgcgtttt tgattactgt acgaaccaaa ctttctgcca
    29881 tttgatccac caaatctaaa gtatcataag gcgtaaaata tctttcaaac gtatgcataa
    29941 ttatatctgt acatccacac attgtttgat acttagatac tgtatatgtt aattctggat
    30001 ttaaaataga aaatttaaaa tatccatatt cactactcaa tccacgtttt agatttccat
    30061 cctcatttgt gatgacactc gaattactca tttcactacc cgtagccgca agcgttaata
    30121 tgcatcccat tggtaaagca ccctttacct ttttctttct tgcataataa tcccagacat
    30181 taccttcatt ataaacacca tacgcaatgc ccttacttga atctaataca cttccaccac
    30241 caacagctaa aatgaagtca acaccttctt ctttacaaat tcgaatacct tcatttacct
    30301 ttgaaagtaa tggattggca actacaccac cacataaaca atacgcaata ttttcttcat
    30361 ccaacgcctt ttttactcga tccaaaaggc cagatttaat agcactttgt ccaccataat
    30421 gaatcaacac tttctttgga tgaaattcct ttaaaatctt tccaaccctt ttttcttcat
    30481 ctttaccaaa atacactttt gtaggtgcat aaaacttaaa attttccata attacccctt
    30541 aaattctttt cgttgaaaca acttcaatca acaagctaat acctgccaca atgagatagg
    30601 cagctaaaat ataattgatg actaaagcac tttggaaagg cattaccata aagataatac
    30661 ccaatagcaa attgacccaa cctgttaaac ttaaataatt ggtattgatt actctaaaaa
    30721 agcttaaccg agaaccccaa gtgatttttt gaattccatt caccaataaa acaaacgcaa
    30781 acaagaacgt caaagtactt actgtaatcg tgactggcat cgtacataat aaacagccta
    30841 acaagatgtt taagattcca ctagccaacc acatcgcatc tctaaagaag aaagttgttg
    30901 tgatatacga atagatttca caagcacccc aaacaatcaa tccaatagct gcaataattt
    30961 gaatcgttgt gaatactttt tctggaaata caaaacataa aataccaatt aaaatcaaaa
    31021 ccacacttaa tattatacct attcttttaa tctttttata cctttcattc cattgtgttg
    31081 tgaattcatc aatataatca taatccgaat caaaccattt catatgtaac atctccttta
    31141 tgacttcagt atatcaccat ttttagcact ttcaacataa cagtgctaga catattacaa
    31201 tatctgtcta atattgtaaa tttatgtcat caatgataaa cttttacttg tcgaaaaaag
    31261 gagacttgta tgttaaaaaa atacttaatt gtgtttttgg tatcaatggt accactcatt
    31321 gaacttcgtg gagccattcc tattgccgct ggaatgggat tgccatttgt acaatattat
    31381 attattgcga ttattggaaa tatgattcct gttccattta tctatttctt tgccagaaag
    31441 attcttgttt ggggagccga caaacctgtt attggaaact tttttacttg gtgtttagaa
    31501 aagggtgaaa aaggtggaaa aaaactacaa gaaactgccg gaaaagggtt atttgttgcc
    31561 ctactattat ttgtaggtat tccattacct ggaacaggtg cttggactgg aacacttgcc
    31621 gcaagtattt tagatatgga ttttaaatca agcgtactcg ctgttatgtt aggcgtcatt
    31681 cttgcaggta ttattatggc tttagcaagt atgggcttat ttggagcact tggagcacta
    31741 ttttaagtag tgcttttttc ttttgtctaa aagaaaaagg tttgaaaaaa aatcaaaccc
    31801 tacttaaaca tattatgtct aaaaaagtat acggtcgcaa ccacacaggc caaaatagcc
    31861 acaaagcttg ggaaccatac aaatggaaat ggcaactgca cattcattcc gtagaaaccg
    31921 aaaataatat tggggatggc cattacaatt gtgattacag ttaatttgtt catgacaata
    31981 ttcatattgt tagaaataat acttgcgaat gtatccatag ttcctgccat tgtatttgaa
    32041 taaatattac acatttcaat ggcctgatga atttcaatca tgacgtcctc taacaaatcc
    32101 tcatcttctt catacaattg aatttgtttt ccacgcataa ttttatttaa tgttacttca
    32161 tccgatttta acgaagtcga aaaataaacc aatgattttt ctaaacctaa catttcaatc
    32221 aactcatcat tttctaccga ttgatgtaaa cgcgtctcag cacgacttga taaacgatca
    32281 atttggcgta gacaaatcaa aaatctttgt gaaatacgca aagctaaaag taagaagaac
    32341 ctcgttttta aacgcgtatc catacctttg attcttcctt gtgacatttc taaaatactc
    32401 cagttttcat acaaactaat tgtgactaca tatcctttta aaatcacaat accaattggc
    32461 aatgtggaat aagttgaatc aatcgaatca tcatcttcat tttccgcact tggatagtca
    32521 aaaataatca acaactgtct aaaatcatcg tcatagtcca tatgcgagct ttcttcttca
    32581 tccagtgaag atttgacaaa ttcaggtaaa acaccaattt gttcaatcaa ataatttctt
    32641 tcttcttctg taggcgcaat cgcattgatc caacagcctg gttctggttc ttgaattttg
    32701 cgtgttccat cttcaaatgt tttataaaat tctaacacaa tttgtcctcc ttatatctta
    32761 aacgaagaca aatcccatgt ctccgaacat atatacattt ttattattaa aataactcgg
    32821 gtctggagac atgtttccac ctactttcta ctaaagaata ttgtatcata ttcatgtcaa
    32881 tttcttgtaa attttcacaa aaaatgacta gtaaaaacta gtcataaggt ttgtattcca
    32941 ctgtttcttt gtcaaaatca ataaacaatt ggaatgcatt acgatctttg tcttcatccc
    33001 agatttcaac aaacttaggt gtaacttcaa ttagaatttc tgtatctaca tgtgaatatg
    33061 cattgtagct ttgccacaag aattctttgt atttcttttc aaaaatacga ccgggttcat
    33121 caataaccaa tcctaaattt ttagctgtac cttctacttg cacaccatta aagcacatag
    33181 ctactctatc atttttgaat aattgttgtg tttttctaaa gtttttatct gtcttaaaat
    33241 aaattttatc attgtagaaa acacaactta catttcttac cataggataa tcatctacac
    33301 agcttgccaa agccatgatt ttataatctc ctaatttttc atccataatt ttttttgctt
    33361 ctaaaagttc catttttctt ttaacctttc attactaata ttaaaacaac aattgacaca
    33421 ataataaatg cactcacaat tccaacatct tttgtaagag tttcattcgt gttcgattga
    33481 ggaacaaact gtgtatttct caatgcgttt acaagtggtt tgtagatcaa catcgcacat
    33541 gcagcattca atgtacactt aataatattg aatggaacaa tacctggaag taacatacca
    33601 acgactgcac ttcttggcat ttgataataa attggagtaa taatatagtt ccataaagtc
    33661 atactcaatg tgcaacatac agttcctaat accaaacctg caatggctcc ttttttagaa
    33721 tgtttatatt tgtagatgca agcagccaca caagcgaatg tacaagtaga aatcacattc
    33781 atcaaaacat ctaataagtt tcctccacgg aatacaattt ctaaaaatga acacaataga
    33841 ctcataataa aggatgtcat aggaccatag ataaagcctg caatcacaat aatgatatct
    33901 tttggatcgt agcttagaaa agatacggct ggtacaattg gaaattgtat caataaattt
    33961 aaaaccattg ccatcgcact taagattgca atggttgtta gtttctttgt tgaactcata
    34021 aatcatcaat cccttcatat atcaactaaa aaacgtgcct aaagaaaatc ctctaggcgc
    34081 gtttaaatat acaaaagaat atataaaaac atcttcttcc atccagacta tactgtcgcc
    34141 actggaattt caccagttcc tgcaaaatgc tcgcggggtt taccgccgat agggaatttc
    34201 acccaaccct gaagatttaa tttatgcaaa ttaagtatac acttgtttta gataaaaaaa
    34261 aagagcaatt ttttttgctc tttatggttt cttaaatcat aagccatgtt ctgttccact
    34321 tacgcggtga cagtcattta tctatagcaa tgctatccag ctttcctttc gtttcattcc
    34381 agccggttcc cctaccaaat ttgggtttct cgcttgtggg gtttacccgt tccaccctat
    34441 acgtttccat atagattcgt ctctgtggca cttttaaagt ttgtttacat agtttccctt
    34501 agcaaacaaa acagccgtca agtaaaaact tgcctagact tatttattca tctagcacaa
    34561 acactacaat catcacagat tgtgcgagca tggactttcc tctaactatc taagtagcca
    34621 gcgactgtcg atttaatacc tattcttcac tttgattgaa tacagctttt tccaaacgtg
    34681 caatacgttt taagatatct actgtattgc tgcttccatt cacaaaatca gaatttactt
    34741 tttcgtttaa gttttcattc tcactttgta acgcaaacaa ctgttgctgc aattctttga
    34801 ttttttcttc ataacgagtg actgcagcac ctaattcttc tactttttca tcgtatttct
    34861 gatagtcttc aatgaccata tctagaaatt catctacttc agcaggggca tatcccttca
    34921 agtctacatg aaattctttt tcataaattt cttgcgctgt caaattcata tctgttgcca
    34981 tatatatagt ccccctatat ctacttagat attatgaagg ttttaacaga aaaaatcaac
    35041 tctgagtttt gagtttcatt gaatgttcac tctttttcat atataatgtt cctgatgagc
    35101 actattaaat atccgaatgg aatttcgacc ataaatacaa tgcaatcaaa gagttatagt
    35161 tcaagaggta tgcgccttga gaatgatata aatgactcca ataaatatta tcgggagctt
    35221 gataaagctt tgatttataa aaagcctacg ccggttcaag ttgttcatgt tgattatccg
    35281 aaaaggcaac gtgcaaaaat tactgaggct tattataaaa cgccatctac aaccgattat
    35341 aatggtgtat atcgaggtaa atatattgat tttgaagcaa aggaaaccaa aaacaaaacg
    35401 agtttcccac tctttatgat tcaccctcac caaatcaaac acctaaaaaa agttgtattt
    35461 catggaggta ttggtttttt tatcattcga ttcaattacc ataatgaaac ctatcttgta
    35521 gattcaaaga tgctcataga taaaatcgaa aatatagata aaagttccat accttatact
    35581 tggtttcaaa atcatggaac tcttattcaa gaaggattat atcctaggtt agcgtattta
    35641 aaagttttag atgaactcta ttttaaggag gataagtaaa tggcccaacc acaaaatgaa
    35701 caaaaaaagc caacagcacc aaagacaaac agaagaaaat tagatatatg gaataaactt
    35761 gccgtaagca tcctatctat attcttagtt ggatgtatat ctgtattctt tgtacttgtg
    35821 aatattatta atgatccaga cggcatgcgt ttttcacaag acggcttaac aacattgtcc
    35881 aattcaagat tatatgactc ttcaggaaat ttattttatg aactcggtga tgaaatccgt
    35941 gaagacgtta catattcaca gattccacaa tcggtagtgg atgccttcct ttctattgaa
    36001 gattcacgtt tctttgatca caacggattt gacttgccac gattcttaaa atccgcaatg
    36061 gcaaacttaa aatctggtag tttttcacaa ggtggttcaa ccctaactat gcagatgatt
    36121 gataatgcat ttacgaaaaa ccaagaaaaa aagattgaaa cggaacaagg ctctgtttca
    36181 acagttcaaa agttaaaatt aaaagtacaa gaaatctact tatctttaat tgctgaacaa
    36241 agtatcaaca aagaacaaat atttgaatat tatgtaaacc gaatttggtt tggttccgga
    36301 aacaatacgc gtggtattca aaaagcagca caatacttct ttaataaaga cgtatcccaa
    36361 ttaaacttag gtgaagctgc attcttagct ggttgtatca atgcaccaga cacatataac
    36421 ccattaaaca atttaaacga agccaatacg acaacagatc acttgaaagc agctcaaaat
    36481 cgacgtaatg tgactttgga attaatgatg caacatggat atattacaga agaagaatat
    36541 aatcttgctt caaatcaaga catatctttc tctttacaat atattgaaag aacaagtgat
    36601 gatccaaacc aagcttatat tactcaaaca cttagtgaag ttatggaatt gactggacaa
    36661 gaccctgcca ttattccaat ggatatctat accgcattaa atcaagatgc tcaaaaacaa
    36721 gccgatgaaa tctgtaatgg taatatcgta caatatccag atgaagcatt tgatgtaggt
    36781 ttctcaatga ttgaaaatga tacaggagaa atcattgcgg taggacctgg acgccgttac
    36841 cactctgact ctgttaaaat tgataactct acagattcaa aacaacctgg ttcaacaatg
    36901 aaaccattgc ttgcctattc aagtacattt gatattcttg gctggtctac agttcaccag
    36961 gtaaacgata agaaaaagga ttattggaag aatggttctt atgcaccagt aaactcagat
    37021 ggtaaataca atggtaaaat gagtttacaa gacgcattag gcgtatcaaa aaatacaaca
    37081 gccgcgcaag ctatgattga tttagtaaca gccaaaggct atgattactg gatcgatttc
    37141 tgtaagaagc ttggttatga tgaagatgta gctaatgcat tcaatgaaca atatgcgatt
    37201 ggtggttctg gaatgtatgc gagcactgtc caacaagcaa gtgcctactc tatgcttgcc
    37261 aatggtggta aacgtgtaga tgcccataga attcgtaaaa ttattcgtag atctgataaa
    37321 aaagaatttg aaacaaaagc tcaaacacat gaattaatta gtgaacaagc cgcttggatg
    37381 atgtcgcaat tgcttgaaaa agttgttagt ggtggctaca acaactacaa caacatccta
    37441 atgagtaatt atacagttta tggtaaatca ggtacttccg attggcaaaa cgaaggtgct
    37501 caatatggta ttcctgctgg taccgcaaaa gatgaatggt ctttaggtta tacaaacaaa
    37561 tatacgattg cttgctggag tggatataca acagaatata ttcaacaagg ctactacatt
    37621 acaatctatg atttaaatgt tgcgactgca ttccatattt ctcactatat gttagattac
    37681 atgcaaaaat atgcgacata ttcagctatt gaaagaccaa gtggtatttc cgattataag
    37741 aacggatata tcaaagatga attcaaatca aaaggtgata cagcttctga aagcacagac
    37801 gcacaagaag catgtgttgc cgatggtggt gaatgggatg aagaagctgg aacttgtaag
    37861 aaagctgacg atgaagaaaa agaagcttgt gttgccgatg gcggaacttg ggatggaact
    37921 acttgtaaaa aagaagatac agaaaccaac gatgctgaaa caacatgtgt aaataatggt
    37981 ggtacttggg atggaagtgc ttgtacatat ccaactacac cagatcctga accaacacca
    38041 acaccagatc ctgaaccaac accagatcct gaaaatccaa caggatatgt ctggtatgat
    38101 cgaagaagtt tatataacgt atttgataga ctttagtaaa agtgctcaat tgagcacttt
    38161 ttttgtatac aaaaaaacag ggataatcat ttccctgttt caagtatcgt attcgcaatt
    38221 tgaagttgtt gatcacttgg cgttggtatt ccttctaaag gatattctaa acctaattct
    38281 ttccatttgt aagcccctaa cgtatggtat ggaaggactt ccactctttc tacatttttt
    38341 aattgagcaa caaattgatg taatcttttt aaatatactt catccgtatt aatagttggc
    38401 actaacacat gacgaatcca tacaggtttt tgaatgtctg ataaatattt agcgaattct
    38461 aaaatatttt gattggtatg cccagttaat tctttatgcg ccttttcatc aatatgctta
    38521 atatctaaga gaactaaatc tgtaactttc ataagttgtt taaatttaga aaagaaaggt
    38581 tcttcaaaag taaagggctg tccacaagta tctaaagttg tatggacccc ttcttttttg
    38641 gcctgtgtaa aaagctcaat caaaaaatca atttgaagaa gtggttctcc accactgaca
    38701 gtaataccgc cattctcttt ccagtaagga cgatatcgca aagctttctt taaaacagcc
    38761 tcagcacttt gattgggttc ttttactttc caagtatccg cattgtgaca aaacttacaa
    38821 cgcatatgac acccttgcat aaaaatgaca tatcttacgc ctggcccatc tacaaggcca
    38881 aatgtttcaa ttgaatgaat ggctcctaac atcctgtcct cctaactaga aagagcctta
    38941 ttaaaggctc ttatgacatg tacgactaat aacatctaat tgttgttcac gagttaaatc
    39001 aataaattta acagcataac cagaaactcg aatagtaaag ttagcatatt cctctttttc
    39061 tggatgttcc atcgcatctt ttaatttttc tacaccaaat acattcacat tcaagtggtg
    39121 agcaccctga tcaaagtatc catctaatac attgactaag ttattcactc tttcctcttc
    39181 actatgaccc aacgcatccg gattgattgt ttgcgtatta ctaattccgt ctaatgcata
    39241 ttcataagga agttttgcga cagaatttaa tgaagcaagc aatccatttt gttctgcacc
    39301 ataagcaggg ttagcacctg gtgataatgg ctctccagct tttcttccat caggcaaagc
    39361 accagtagct ttaccatata caacatttga agtaattgta agaattgaag tagttggttc
    39421 actatcacga tatgtgtggt gcttttcaat cttacgcata aatgtcttta ataaccatac
    39481 ggcaatttca tccgcacgat catcatcatt tccgtagcga gggaaatctc cttcaatttc
    39541 ataatcaaca accaatccat cttcatcacg aattgtctta acctttgcat atttaatggc
    39601 actcaatgaa tccactacat gagagaatcc agcaatacct gttgcgaaag ttctacgaac
    39661 atctgtatca attaaagcca tttgacttgc ttcataataa tatttatcat gcatatactg
    39721 aattaagttc aaaatattta catataaacc tgataaccaa tccatcatga tatcgtactt
    39781 atgcataact tcatcataat ccaaatattc acttgtgatt ggtttatatt ctggacccac
    39841 ttgaactttt gtcttttcat ctataccacc attgatcgca tataataaac atttagctaa
    39901 gtttgcacga gccccaaaga actgcatttc tttacccgtt tgcgttgcac ttacacagca
    39961 acaaaccgca taatcatcac cccatacagg acgcatcaca tcatcgtttt catattgaac
    40021 actacttgta tcaatactta cttttgaagc atatttcttg aatggttctg gtaaatttga
    40081 agaatatagt acagtcaagt ttggttctgg acttggtccc atattttcaa gtgtatgcaa
    40141 gaaacggaaa catgttttag taaccatgct tcttccatcc atacctaaac caccaatatc
    40201 tagcgttgcc caaactggat cgcctgagaa taattggttg tagctaggaa ttctggcaaa
    40261 cttaaccatt ctaaatttca tagttaaatg gtctacgatt tcttggattt cactttctgt
    40321 aaagacacca ttttctaaat ctctttcaaa ataaatatct aagaaagtcg aaatacgacc
    40381 tacggacata gccgcaccat tttgtgtttt aatagctgct aaatatccaa aatatagcca
    40441 ttgtacagct tctaaagagt tcttagcagg ttgagaaata tcatatccat agcttgctgc
    40501 catgactttc atttctttta acgccttgat ttgttccgcc aattcttcac gctgacgaat
    40561 cacatcatcg ctcattgttc catcgccaca atatgcgaaa tcttttcgtt tctgctcaat
    40621 taagtaatca ataccataaa gtgcaacacg acgataatca ccaacgatac gtccacgacc
    40681 atatgtatct ggtaaaccag tcacaatctt gttgcgacgt gctaaacgca tttctggtgt
    40741 gtatgcacta aatacagcat cattgtgagt tttatgatat tttgtgaata tttcatgtaa
    40801 tttttcactt ggtttatatc cataagtttc gcatgcctct tcactcatac gaataccgcc
    40861 ataaggcata aatgcacgtt ttaatggttt atctgtttgt aaaccaacta ccttctctaa
    40921 atctttatct aaatatccag ggccataagc tgtaagactt gaaaccactt ctgtttccat
    40981 atccaatacg ccacctttgg cacgctcttc tttttgtagt ttttgaagtt tttcccacaa
    41041 agtatttgtc gattctgttg gaccttctaa aaagctttca tccccatcat aaggcttgta
    41101 gttgttttga ataaagtcac gaacatctac ttcgtcttgc catttactgc ctttaaatcc
    41161 ttgccattgt tctttcatga ttgcctcctt caaatgaaag ttagtttaaa ctaacaattg
    41221 attaaagttt actatgattt cttcgagtag tcaattaaaa agacccaccg aaaagacaaa
    41281 gtaaacttgc ccagacggtc ggcctataac atcatacttc atgtggtaac tccacttccg
    41341 tcagtcatat gatattcaaa tcataacaaa gaactatcca ttacgcaagc gttttcatca
    41401 tggacatgaa tttagctttg tgcttctcca atatttgtca ttttaaaccc ctctccatgc
    41461 acttcatgcg attcgacaac cacaaagaaa gattctggat cctcttcttg aactgccttt
    41521 cttacttgat acatttcttt actgttacat gcacacatta ccacttgcat atcatcttgt
    41581 ttatatccac cttgtgcatt taaaatcgta cttccccttt gacttgtttc atcaatcacc
    41641 tgacaaattc tttttccctg ttttgtgaca ataaaagcaa ctttacctga atttaagcca
    41701 taaatcattt tatcgactac aaccgcaaat aataaattta caatcatacc ataaatcatt
    41761 ccatctacat ctttaaatag aataccaccc actagaataa ttccaacatc tgataaaaag
    41821 gcgatatttc ctaaagataa atgtggtctt aatgccttta cagccatgat gataaaatca
    41881 gatcctcctg tagatgaatt tcttgtataa atcatcgcat agccaattcc accaaaaact
    41941 cctgtacata gggcagctaa taatcgatct ccttggtaga ctggaaataa tggtgccaca
    42001 tagtcgataa tcaatgaaga tacaatcata cagcgaatgg aacggataaa aaacttcttt
    42061 cctaataatt tgtaacaaag tatcgcaaca ggtatattca atagaatggt tgaaaaacca
    42121 ataggaatat gaaagagtcg ataaaagata attgaaattc ccgaaaatcc tgtcatagga
    42181 aacttagctt gtacggcaaa attataaatt ccaatcgcaa caaagatact gccaactaat
    42241 tcccacatca tttgtaccat taattctttt gtcttattca taaatacccc cccaaaaaaa
    42301 gaagcgccta agaccaaatc ttacagtgaa atcactgcac ctacgatttg atcctagacg
    42361 ctttaaccac tcatccggtg aagagtgaac gttattttta atgcaccaat ctattgtatg
    42421 gaacatgatc atttaagtca agaaaaattc tatatttgtc atttaaagtc tatttaaagt
    42481 acactttcaa agtatacctt tacggtgtac tttaaattaa attcaaccat tcgctatttg
    42541 tggtatgttc aatctatact agaaatagta agattttaga tgaaaggagg cccattatgg
    42601 acactaaaaa gataggagcc tttatcgcat tcaatcgcaa gaaaaaagga tatacacaag
    42661 agcagttagc tgaaaaatta ggcgtgacaa ataaaaccat ttcgcgatgg gaaaatgggc
    42721 attatatgcc agacttatct ttacttgaac ctttaagtaa agaattagat atcacattaa
    42781 atgaactttt agctggagaa gaaattgtta aagaagaggc tatggagtat tctgaacaaa
    42841 acctaatcca aacaattgat tatacagaca agaaaatcaa gaatgagcat aagaaaatat
    42901 cattattcat tattggcata ggtattttaa tatccctttg cgcctttact gttttccctt
    42961 ctgaaagcag ctggggcagt atctattcta tgattggatt gttcttattt gtagttggaa
    43021 tatttagaga attaaagatc acttctctac tcaagaaagg attgatttca acaattttat
    43081 tcattttact tttaggcatt ttctttattt tcgactatgc cagtgtaagc caatttaaac
    43141 aaccacccat ctatcgactg actacaacta ctgtatttag tgatgatgga aataaaatga
    43201 ttgaatacca aaatccattc tataatgtat ttcgcatcaa tgccgataca cccaatgaat
    43261 attacttaat tgacaacaag agacaatata ccattgatac cgttccaaca agtcctttta
    43321 atgtggataa aagttcaatg aaacaattga agcactataa aagtcattat attggggata
    43381 atagtaatac atcccattta cttagtgcct tgccactatc ggaatatggc tatgaatttg
    43441 aaattgattc tgaaaactat ggacttaca
//
LOCUS       DBMDWL010000002        35348 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_485_length_35348_cov_3.491673, whole genome shotgun sequence.
ACCESSION   DBMDWL010000002 DBMDWL010000000
VERSION     DBMDWL010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 35348)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 35348)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35348
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_485_length_35348_cov_3.491673"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            27..100
                     /locus_tag="ACZPDN_00185"
     tRNA            27..100
                     /locus_tag="ACZPDN_00185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Gly,seq:tcc)
     gene            complement(154..1047)
                     /locus_tag="ACZPDN_00190"
     CDS             complement(154..1047)
                     /locus_tag="ACZPDN_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1297077.1"
                     /translation="MIIKKITSKQDGLKLEIAIMECKNPKGIVQFSHGMAEHKERYFD
                     FMQYLNERGYICVIHDHRGHGGSVKSKNDYGYFYTENADYIVDDLFQVTEYVRDMYPG
                     LEVTLFSHSMGTLVARCYLKKYDEYIRCLILCGPPTENSAASFAVGLAKIIKPFFKEK
                     KGNGLLNKMAFAGYESGNRWLSKNLENVDQYEADELCGFLFTTNGFINLFQLMVKAFD
                     KKDWKVTNGELPILMIAGQEDPVIQGKDKFEQLKQFLMGVGYKNVSSKLYPTLKHEIL
                     NEKENQRIYEDVYSFIKDAKD"
     gene            complement(1148..1357)
                     /locus_tag="ACZPDN_00195"
     CDS             complement(1148..1357)
                     /locus_tag="ACZPDN_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297078.1"
                     /translation="MKSMLEELKDVKIKKHTVTSIEYDCKSEEKEDEVFDQVHNIVTN
                     HLDDFAKITFDVEADHKVKVEISEN"
     gene            complement(1409..2752)
                     /locus_tag="ACZPDN_00200"
     CDS             complement(1409..2752)
                     /locus_tag="ACZPDN_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297079.1"
                     /translation="MLGLAQGQKLMTSGSIPKQMVFFALPILLGNLFQQLYNTVDSLI
                     VGNFLGSQALAAVSSSGNIIFLMIGFFNGVAIGAGVVISRYFGAREIERMQKAIHTTV
                     AFGLIVSFFMTLIGIFFTPTLLRWMSTPESVMDASVTYFKIYFLGSFGSIMYNFFVGI
                     LQAVGDSKHSLYYLIVSSIVNIVLDLFFIAILKMGVGSAALATIISQYISAGLCLYLL
                     LTTSGTHRIVLSKIRIHKEFLGEILKYGLPSGFQNSIIGLANVVVQSNINSFGDMAMA
                     GCGAYSKIEGFAFLPITSFTMALTTFVGQNLGARQYDRVLKGAKFGMICSMTLAEMIG
                     VIIFVFGSQFIAAFDATPEVIQFGTLRGQTSAFFFCLLAYSHCVSAILRGAGKSMVPM
                     IVMMVCWCFVRVAGLTIMNALVHNIWCIYWIYPITWSLSSITFFIYYHRIDWLHA"
     gene            complement(2781..3722)
                     /locus_tag="ACZPDN_00205"
     CDS             complement(2781..3722)
                     /locus_tag="ACZPDN_00205"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR1297080.1"
                     /translation="MIKKRKVVIVGAGNVGASTAFCMINQGICDEIVLIDMNTERAKG
                     ETLDMIHSIAFMNRNMQIQLGDYTDCKDADVLVITASAPMGKENDRLVALKSSSKIVT
                     SIVSSAMENGFNGFIVVVSNPVDIMTYVAYKVSGLPANQIIGSGTLLDSARLQCHIAD
                     CIDVDTKSIHAYVLGEHGDSEMIPWSTVRVGGKDIYQIIKDNPTRMNEGMFDSIHEAV
                     KKDGWEIFNRKGNTCYGIAASTTRIVRALMFNESVVLPVSTYLDGQYGQDGVFTSVPA
                     ILDATGVKEVVEIEMTKEEKEQFDSSCSHLQSFYKEV"
     gene            complement(3734..5092)
                     /locus_tag="ACZPDN_00210"
     CDS             complement(3734..5092)
                     /locus_tag="ACZPDN_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1297081.1"
                     /translation="MRDRFSNRLGFILISAGCAIGIGNVWRFPYIVGQYGGAAFVLIY
                     LFFLVILGLPVMVMEYSVGRASQKSAALSFDVLEPKGTKWHFMKYVAMAGNYILMMFY
                     TSVCGWLFYYFYKMLIGDFNGLNASQINDEFGLMLSNSFVQVGMMFLVVCIGFFICSK
                     GLQNGVEKVSKYMMLCLLVLMLILAIRSLILPGAMAGVKFYLLPDFSKMQNISEVVFA
                     AMGQAFFTLSLGIGAIAIFGSYIDKSKKLTSEAITVVCLDTFVALVAGFIVIPACFAY
                     NVDPGQGPGLIFQTLPNIFANMQLGNVWGALFFLFLSFAALTTIIAVFENIITMTMEW
                     TGWSHSKTIKVSFVLVFVLSLPCALGFNVLSFIQPLGAGSTIQDLEDFIVSNNLLPLG
                     SLCYVLFCTSKYGWGFKNFLKEANCGEGISFPKQVGFYVSYIIPVIILVIFIQGYISK
                     FL"
     gene            complement(5089..>5818)
                     /locus_tag="ACZPDN_00215"
     CDS             complement(5089..>5818)
                     /locus_tag="ACZPDN_00215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIR1297082.1"
                     /translation="QSIFRFDKNIDFYILHQDLNQEHKDDLMQSFPDCTFHFIKVKEE
                     SFKDFPTSSRYPLEIYYRLFASDLLPDTLDRILYLDVDIVVIQSLRELYNMDFQDNLY
                     IACSHVSDNMTHLNAKRLGLKEDVPYINTGVLLMNLIALRKQLNKQDVLNYVNAYKKK
                     LVLFDQDVLTALYGDKTRLVDYRKYNLSERMMNFYNLRNPRNKMDLDWVKENSVIIHY
                     CGRMKPWSGKYIGCLDYFYRELEK"
     assembly_gap    5819..5828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5968..6975
                     /gene="asnA"
                     /locus_tag="ACZPDN_00220"
     CDS             5968..6975
                     /gene="asnA"
                     /locus_tag="ACZPDN_00220"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR1297083.1"
                     /translation="MKLIIPENYDPVLTVRETQEAIKYIRDTFQYEFGKELNLSRISA
                     PLYVTQSSGLNDNLNGTEKPVSFTMKELPGEQFEVVHSLAKWKRMALKKYGFKLHEGL
                     YTNMNAIRKDEELDNFHSTYVDQWDWEKIISKNERTQQTLEEHVRTIFKVIKHMEHEV
                     WYKYPQAVNHLPDDIFFITTQELLYKYPNLTAKERENAITKEHGCVFVMQIGDKLSNN
                     EKHDGRAPDYDDWTLNGDILFWYEPLQSALEISSMGIRVDEDTLLEQLKKENCLERCE
                     LPFHKAILNKELPYTLGGGIGQSRLCMLLLKKAHIGEVQASLWPEDMIDTCLKNNIQI
                     L"
     gene            complement(6986..7273)
                     /locus_tag="ACZPDN_00225"
     CDS             complement(6986..7273)
                     /locus_tag="ACZPDN_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297084.1"
                     /translation="MEKYFELSRAYYTYLKNSTKKNQCTVSVICDDIDIKHIIMDGLL
                     FDGAFIYDGYCENIDGTITVCDGNISLSGCELADLVYGNYASNSNGTIIKK"
     gene            complement(7321..11214)
                     /gene="rpoC"
                     /locus_tag="ACZPDN_00230"
     CDS             complement(7321..11214)
                     /gene="rpoC"
                     /locus_tag="ACZPDN_00230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1297085.1"
                     /translation="MSINNFASIQVRLASPETILKWSHGEVTKPETINYRSQKAERDG
                     LFCERIFGPTKDWECACGKYKKVRFKGVVCDRCGVEITKASVRRERMGHIHLAAPVAH
                     IWYLRGIPSRMALLLDVTPKQLEEVVYFVSYIVTDPKESGLAYKQILSEREFRENQAI
                     YGSTGFTAQTGAEAVLRLLQDVDLESEYQEVQDALEKAQGEKRKKLIKRLDTINAFRN
                     SENLPEWMILTNIPVIPPDLRPMLQLDGGRFATSDLNDLYRRVITRNNRLRKLLDLGT
                     PNIIVQNEKRMLQESVDALIDNGRRSKPITGAGGRALKSLSHSLKGKQGRFRQNLLGK
                     RVDFSGRSVIAVGPDLKMYQCGIPREMAVNLFKPFIINEIVNQGLAQNPKNAEKLIER
                     RDPRIWDVVEKVIDGYPVLMNRAPTLHRLGIQAFLPKLVEGRAMRLHPLVCTAFNADF
                     DGDQMAIHVPLGEEARAEALVMMLGSHNILGPKDGKPIVTPGQDMVMGNFYLTMEETK
                     DEFFAEAKHYEEVGCPLEATHWHRFGENEGHVYTDEDEVMMAYQSKQVHLHTRIALPV
                     CNMHKTSFDEKHKNDYLITTVGKIVFNSMFPEDFPYINEVSKENFVSTPDKYFVSPGQ
                     NIKEYIEAMPLVDAVKKKDLGKVIAEVFKRYDADQTAEILDQIKSLGFEYSTVAGITV
                     ALSDIEVAPHKDEYIDEGRVKADQLKHLQRKGMLTMEEWERHLSKMWDDQKDKIVTSL
                     MKNLPRKNPINMMATSGARGNASNFTQLAGMRGLMAKPGHAKAGAGEYVPTIIEVPIY
                     SCFREGLNVSEFFISTHGVRKGLTDTALKTAESGYLTRRLVDVAQDVIIKEDDCGTDK
                     GYWIETLMDRKTNSVIEPLQDRLVGRYSKQDVTDPKTGELIIASDEFITDELAKKIVD
                     AGVTGMYIRSVFTCKSRLGICRKCYGRNMATGKDVEVGEAIGIMAAQSIGEPGTQLTM
                     RTFHTGGVAGAGAEDITQGLPRVEELFEARCPKGVAVIAQISGEITSIERIEGTMRQE
                     VIITNEHESVSHKINANQSMRPWVQIGAKIEAGVALTEGPLDPKELLRVAGVREVQDY
                     ILKEVKKVYQSQGIEISDKHLEVMIKQMMKKVIVVESGDTDLNVGVQLSLNNITKINR
                     EALLSGKTPATFKPVLLGISKSSVETDSFLSAASFQETTKVLTDATIKGKVDHLIGLK
                     ENVIIGKLIPAGTGCQGDRPQNDIVAAKAKELRDKRIARMNEVHNEDSEKFDKLVSGS
                     DDRDMMDTVDSSVEESILQDAETTDNGSIDIQSEE"
     gene            complement(11223..15320)
                     /gene="rpoB"
                     /locus_tag="ACZPDN_00235"
     CDS             complement(11223..15320)
                     /gene="rpoB"
                     /locus_tag="ACZPDN_00235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1297086.1"
                     /translation="MDNNKAYRIVSCGKKSSRRDYSKVSGKLELPNLVEIQTDSFKWF
                     TQQGIQEVFEEIYPIENYGKNIRLNFLRYHFEEPKYNAEESMYRECNFAAPLYADMEL
                     EVTDSETGEVVTKSEEVYLGDFPLMTETGTFIINGAERVIVSQIVRSPGAYFADSYDE
                     KTGKQNFSCELIPSRGTWLEFMTEQKKTTNGRLINVSIDRRRKVLFSILFKAIGMSLN
                     IGVNEDTHDTSMMETFLRAMGRNWSDVATDAEDREYMNMYLLLYTAFFGKYEEIENSL
                     LNDKVKTTQEALLSFYENQRSDEIPTLDGSITLMQAKFFDHRRYDLTKAGRYKLRKKL
                     NAIDRMYGMSLAHDIVDVNGNVFMEKGTMVHRDERNALREELAKGTYCVAYPFRSEFH
                     EEDIVSIPTSWTTGLIGRVLASDVETEDAYLDAGTVLTEQDVLAIQKVVENVEIFAGL
                     FAQQVKLTAENMDSVFNYGQRLYALGRLTNAQGEDIVDADMELVANRYMVGVSPDAID
                     SDVETQVKQRALSEDITAWLIGACVQEVYIIDNNGDEVRVAGNDPFANKHTITVSDMY
                     AFFSYSLNVMEGVGTTDDIDMLGNRRIRSVGELIQNQFRIGLSRMERVVKERMSIQEM
                     DSLTPRKLTNIRPLTAAIKEFFSSSQLSQFMDQQNPLAELTNKRRISALGPGGISRDR
                     ASFEVRDVHNSHYGRICPIETPEGPNIGLINNLTTYARINEYGFIQTPYRKVNADGTV
                     SEDTVYLSADEEADYVIAQANEVRDGHLVNERVVARKAGETIIADREEVELADVSPKQ
                     IVSVATACVPFLENDDASRALMGANMQRQAVPLLVPHSPYVGTGIEYKIAKDSGVGIV
                     AKQDGVIEYVDGLRIVVKDNEGENHTYQLRKFARSNASTCINQRPIVEVGEKVEKGDI
                     LADGPSMQNGELALGQNVVIAYTTWHGYNYEDAIIMSERMVSDDVYTSIHVEEYDIDC
                     RETKLGPEEITRDIPNVSEAAVRKLDSNGIIMVGAEVKEGDILVGKVTPKGQSEVSPE
                     EKLLLAIFGEKSREVRDNSLKVPHGGAGIVHSIRVFKRGDGSDLPPGVNMRVKVYIVQ
                     KRKISEGDKMSGRHGNKGVISKILPIEDMPFMADGHPVDILLNPFGVPSRMNIGQILE
                     IHLGYAARKLGVKFSTSVFDGLSNEDLQDVMREASMTVDGKQVLYDGQTGQPFDERIS
                     VGVMYMIKLAHMVDDKLHARATGPYSLVTQQPLGGKAQNGGQRFGEMEVWALEAYGAA
                     HTLQEILTIKSDDIQGRIKTYEAIIKGKDIPEPGVPESFRVLMHELQGLAIDIRLLDE
                     NNNEVDITLDDDDQDAHPVQPLLQEEQKEELVDDIEEENKEEEEA"
     gene            15574..16281
                     /locus_tag="ACZPDN_00240"
     CDS             15574..16281
                     /locus_tag="ACZPDN_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297087.1"
                     /translation="MKKILTIILTCFLSLNLAGCSASKSNDDILTFFNALDNTLNLKS
                     AQINGTLAMKDSKLNIDAQILQKDELQVSSSIGLVAGKNVQNNFLNFYIKDGKTYLNS
                     MGTKTQSTVDKIGLKKNSKLNAYNPFLDLTDDQLCELFDSSKKENDTYTFKVNTSKLA
                     TLLDNMGSVKLDHATLVATIKNEKISHMILNFTGIQTVDDASADIDVSIELSIKKLNK
                     INFPNDLDNYKNETAED"
     gene            complement(16302..17603)
                     /locus_tag="ACZPDN_00245"
     CDS             complement(16302..17603)
                     /locus_tag="ACZPDN_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025644.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR1297088.1"
                     /translation="MKQKKQFKILTKLVAVILCLCLMFTGCITGAAFFVRPATSDLEK
                     RKLTEFPKFTMATFLNGQYFSDISIWYADTYPMRDSLMNLSQEMKNVYGVRQKTMMVG
                     TKKEADKIPTKKKKTKKVEPVPVPEDYSFDEDMQNQILSNLYVKNGAAYSMYYFVQDS
                     ADLYINAMNRFAKRLKGTSKVYSLLIPNNSIVLPDDELKKLGGSDMKQAINYYYKNYK
                     NVTGIDDYSYIEKAKDQYLYFRTDHHWTALGAYQAYKAFCKEKGWKPEELDSFETKRF
                     EPFLGTAYDQLRLPEMEANPDYVDAYIPHATNDMVYWDENGNETNYNVIADVSDWSEG
                     SGYYCFIGGDNPLSVIKNPTKEKGKSVLLIKESFGNSFAPFLVDHYKTVYIADFRCLT
                     MNIVDFCKENKIDDVIFENNISIIGSTQVASTYDQLSRAGE"
     gene            complement(17613..18833)
                     /locus_tag="ACZPDN_00250"
     CDS             complement(17613..18833)
                     /locus_tag="ACZPDN_00250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1297089.1"
                     /translation="MSDSKKSKLNLILACCCLLGLLAIFKYTGFFLNNLKFFIHWPDK
                     IPTIVLPIGISFYTFQLLSYVVDVYKKEVSPQQDYWKLLLYSSLFHQCIAGPIVRYET
                     VQNEIDNRKVSFEDVFYGFRRFSIGLAKKAILANSCASIADTLLPGGLDAIHNQSVLG
                     IWLSMICYMLQIYLDFSAYSDMAIGMGRMIGFHYLENFNYPYIAKSVQDFWRRWHISL
                     SSFFRDYVYIPLGGNRCSKKKWIRNMFVVWFLTGMWHGASWNFILWGLYYLCFLLLEK
                     FYIKDRVPDIWKRVYTLLVIFFGWIIFKYSDLSQLGAVLLGLFGIGVSGFFSMEVKTI
                     FLSNVFFLIFSMLACTTLGKSLHKYLVKKSNTDNVVMVLYSIFDAIVPFTLMFVSFLA
                     LLGNSYNPFLYFQF"
     gene            19178..20386
                     /locus_tag="ACZPDN_00255"
     CDS             19178..20386
                     /locus_tag="ACZPDN_00255"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1297090.1"
                     /translation="MQSIKTIYKIGPGPSSSHTFGPMMASKYINEQYPDATHFKITLF
                     GSLALTGKGHLTDKIILKTLGASKCEIIFDMKTAVEHPNTMIIEVFDNDTKVAQHTFV
                     SIGGGKIEIDGKKGNVDPEIYPHTKMDDIQVYCNERHLRLDQYIDEVEDSDFDSYMNQ
                     IFKQMLKTVNTGLKKTGVLPGSLKIDRVAHALYQSAQSCSDIQDKNSLLLSSYAYAAN
                     EQNASGGIVVTAPTMGSCGVLPSLVYHFYHDQKYPKKTLIQGLKVAGLIGNLIQTNAT
                     ISGAKAGCQAEVGAACSMGAAFVAYCQLQNNEQIECAAEIGLEHHLGLTCDPVGGYVM
                     IPCIERNAVAAQRSVDAARLAKYLRNVKKNRVSFDMVVQTMNLTGKKLPIELKESALG
                     GLAVVVPEKA"
     gene            complement(20423..21073)
                     /locus_tag="ACZPDN_00260"
     CDS             complement(20423..21073)
                     /locus_tag="ACZPDN_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HIR1297091.1"
                     /translation="MFKVKDYMTKNVICAEPDATISQIIDLMKEKGIHRVPVVEKGQL
                     VGLITEGMISNSGTTNATSLSIYELNYLLSKTTVSTVMVKKVISVDENELMEYATQKM
                     LKNNIGCLPVINASGEVTGIVTQNDVFKCFLNVLGWDEVGSRITVSVKDEIGAIGKLS
                     EIFVENKVNINHIGVYSFEDGIANLVIRCDTTQPDDLQADLERKGYKVLECFVRDK"
     gene            complement(21078..21782)
                     /locus_tag="ACZPDN_00265"
     CDS             complement(21078..21782)
                     /locus_tag="ACZPDN_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297092.1"
                     /translation="MLEIKDLHVHYGVIEALKGISLEVNEGEIVALIGANGAGKTTMM
                     QSISGIVKKSGGEINFLNESIMKANPKHIVEMGLTQVPEGRRIFTGMSVYENLMMGAF
                     LRKDKDGIKHDYEKVCEQFPILKERMNQDASTLSGGEQQMLAMGRALMAKPKLLLLDE
                     PSMGLAPILVKEIFNIIEEINKSGTTVLLVEQNAKMALSIAHRAYVLETGKVVMSGTG
                     EELAKSPDVQKAYLGG"
     gene            complement(21790..22569)
                     /locus_tag="ACZPDN_00270"
     CDS             complement(21790..22569)
                     /locus_tag="ACZPDN_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297093.1"
                     /translation="MKTILNVKNLTKNFGGLCAVSNVSMTINEGELIGLIGPNGAGKT
                     TLFNLLTGVYEPSSGLIELMDQDKSIQIGGLKPYKITNLGVARTFQNIRLFKSMTVLE
                     NVKVAMHKNIRYGTLAAILRLPTYYKEEKWVEEKAEELLKEVGLYSLRNELATNLPYG
                     KQRRLEIARALAIQPKILYLDEPAAGMNPQETKELTELIDHIREKYKLTVILIEHDMS
                     LVMKICERIYVLNYGKMIANGTPEEIKQNEFVVKAYLGEEI"
     gene            complement(22566..23522)
                     /locus_tag="ACZPDN_00275"
     CDS             complement(22566..23522)
                     /locus_tag="ACZPDN_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1297094.1"
                     /translation="MRKELLNKANFAWLLLSVVSFVVLTILMKVGIIGSYYSITLYNI
                     FINIILAVSLNLVIGVCGQFSLGHAGFMCIGAYCAGIMVKMMPNMTGLVIGVLIGFVL
                     STIVALIVSIPTLRLKGDYLAIATLGFAEIVRILVQNMEITNGAAGLNGIPKLTTFPL
                     LFVCAVISILVVCNFTHSAPGRACLSIREDEIASEAMGINTTKYKVIAFVIGALLASL
                     AGALFACNFYVIKPDQFTFNKSIDILVMVVFGGMGSMTSSVLAAVAIGIINVVLANFS
                     EIRMIIYGLALVGIMIFKPTGILGTKELSFKKLLDKRKEKSA"
     gene            complement(23526..24404)
                     /locus_tag="ACZPDN_00280"
     CDS             complement(23526..24404)
                     /locus_tag="ACZPDN_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1297095.1"
                     /translation="MTAFLQQLVNGLSLGSIYALIALGYTMVYGIIKLINFAHGDIYM
                     LGAYCAFLITTYCGLGFIPALLISMVFCGVVGVLIERIAYKPLRHATRITALITAIGV
                     SYVLEYATQYIMGSEVRTYPKLLTSASFHLGPVTITMQQVYIFVITVILMVVLQFIVQ
                     KTKMGRAMRAVSVDEDAARLMGIDVDKTISFTFLLGSALAGVAGVLVGIYYNSINPLM
                     GMTPGLKAFIAAVFGGIGSIPGAMIGGLFIGIAETMVTAYGSSLYKDAIVYIILILIL
                     ILKPAGLLGKNVREKV"
     gene            complement(24455..25627)
                     /locus_tag="ACZPDN_00285"
     CDS             complement(24455..25627)
                     /locus_tag="ACZPDN_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1297096.1"
                     /translation="MKGKLLKTFASSAVALSMLVGCSSGSGTASTELKEGDTVKIGLN
                     YELSGAVASYGQAEYKGASLAIKQYNANKDSKYKIKAVKMDNKGDASESTSAATKLMT
                     EDGVAFMVGPATSGASIATYPVADQNQVPVISPSATQVDATMNGDSPYEYAFRICFED
                     SYQGKAMAKFGYENLGKRKAVIINEVSDYGKGLTKVFTQKFEDLGGEVVATESYNAKD
                     TDFAGIITKVKDKGADVLFIAGYYSEAGLIIKQAREAGMDCTILGADGFESEKLVELA
                     GKENLNNCYYSTAYTTVNASDDLTAFVDAYTEEYGEAPNMFSALTYDATNLGLQALEK
                     AGKTGADLQKAIADAKFEGLTGSFTFDKTHSPKKSVLVVNLVDGVQTEAVSVDPNK"
     gene            complement(25807..27420)
                     /gene="groL"
                     /locus_tag="ACZPDN_00290"
     CDS             complement(25807..27420)
                     /gene="groL"
                     /locus_tag="ACZPDN_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864766.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1297097.1"
                     /translation="MAKEVRFSKDARESMLRGVNTLANAVRVTLGPKGRNVVLEKDYG
                     SPLITNDGVSIAKEIELEDKFENMGAKLVYEVANKTNDTAGDGTTTATILAQSMIENG
                     LRQVDRGANPVLMREGIEKAAKAVSEYILSVSKKVESSKDIANVATISSGSEEIGKII
                     ADAMEKVGRDGVISTDDSKGFETELEISEGMQYDKGYVSPYMVSDREKMEATMENAYV
                     LVTDQKITNIQEILPLLEQLVQANRALLIIAEDLENEVVSTLALNKLRGTFNVVATKA
                     PGFGDNQKEMLQDIAIMTGAKFYSKDLNMELKDMQLSDLGTVKKIVVTKDNTTMIGGA
                     GSKEEVAARVSEIEAQMENTTSDYDKKRMAERLGKLSNGVAIIKVGAATESELKEKKL
                     RIEDALNSTRAAVSEGIVVGGGACLVKAYVALKDEVKSDVVDVQKGIKVVFDALSAPI
                     TQIADNAGYNSEEIVERQKAAEENVGFDAKNGKWVDMIESGIIDPTKVTRNALMNASS
                     ISALFLTTEAGVAKIDKEEPAPAMPQGMY"
     gene            complement(27437..27697)
                     /locus_tag="ACZPDN_00295"
     CDS             complement(27437..27697)
                     /locus_tag="ACZPDN_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR1297098.1"
                     /translation="MLQPLHDYVLLKKEKEEKTTASGIILTSGKEKSKLAVVASIGAD
                     VKDAGYACGDKVLYKEYSGTTMKIDDEEFIVIKDEDIIAVSK"
     gene            complement(27810..28205)
                     /gene="atpC"
                     /locus_tag="ACZPDN_00300"
     CDS             complement(27810..28205)
                     /gene="atpC"
                     /locus_tag="ACZPDN_00300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1297099.1"
                     /translation="MIAFKVVTPKGEYLKQDVKSIHAKTVIGETTILPNHMPIFASLV
                     PCKLVLKNENDEEEVYAISGGFLQFNKNEGMILADAIEGKKDIDLERAKKSMERAKER
                     LEKKDSNTNLKRAQLSLERAINRIHVYGE"
     gene            complement(28208..29608)
                     /gene="atpD"
                     /locus_tag="ACZPDN_00305"
     CDS             complement(28208..29608)
                     /gene="atpD"
                     /locus_tag="ACZPDN_00305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1297100.1"
                     /translation="MSQNIGKVVEIIGPVVDIQFSQNNLPDLLTAIEIERPNEENLIV
                     EVEQDIGSDRVRCIAMGSTDGLVRGMDAIDTGKPIQAPVGENVLGRMFNVLGKPIDGL
                     GDVDANTMPIHRKAPAFDEQSTTSEPLETGIKVIDLLCPYAKGGKIGLFGGAGVGKTV
                     LIQELIHNIAKEHGGKSVVVGVGERTREGNDMYHEMKDSGVLDKTVLVYGQMNESPGA
                     RMRVGLTGLTMAEYFRDVEHQDVLLFIDNIFRFTQAGSEVSALLGRVPSAVGYQPTLA
                     TEMGQLQERITSTKDGSITSVQAIYVPADDLTDPAPATTFSHLDAKTVLDRDIAALGI
                     YPAVDPLESSSRILDPLVVGEKHYKVARGVQNILQRYKDLQDIIAILGMDELSEEDKK
                     VVNRARRVRNFLSQPFNVAEVFSGIPGKYVPLEDTIRSFERLLAGEFDDLPEQAFMFV
                     GTIEEAQKKAEKMAGE"
     gene            complement(29621..30469)
                     /gene="atpG"
                     /locus_tag="ACZPDN_00310"
     CDS             complement(29621..30469)
                     /gene="atpG"
                     /locus_tag="ACZPDN_00310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1297101.1"
                     /translation="MAQSRQAIQARISSVSSTKKITKAMQLIASSKLTRQRQIMEENR
                     SYAQGLQELFTMVLRSAGNDSMYLNENQGKPSFIFVITSDMGLCGGYNANVYRMIQDE
                     FSNEDHIVMVGVRGAAWISKRNYNVETVLSDLDEDDVYNALSKCMQDALDLFEKKEIS
                     KIQILYTHYKNTLTFVPTLETVLPVSKPQEEKKSGMHADMIFEPDKETMLQNMIPMVT
                     KSILYSRYLESKTSEQASRRMAMESATDNAEELQNDLELAYNRVRQGAITQEITEIVG
                     GANALS"
     gene            complement(30456..31991)
                     /gene="atpA"
                     /locus_tag="ACZPDN_00315"
     CDS             complement(30456..31991)
                     /gene="atpA"
                     /locus_tag="ACZPDN_00315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1297102.1"
                     /translation="MSLNPAEISKLIKAQIQSIDQDLEMNESGTILTVGDGIATVYGL
                     KNAMMSELLLFPHDVYGMVMNLEDEQVGVVLLGDNSDIKEGDVVKRTGHIIEVPVGDS
                     LMGRVVNALGQPIDGLGEIQTDKTRPVERVAPGVMTRKSVSVPLQTGLKIIDSMIPIG
                     RGQRELIIGDRQTGKTAIAIDTILNQKGQNVKCIYVAIGQKASTVAQIVEKLKQHGAM
                     EYTTIVAATASEAAPLQYIAPYAGCAMGEEWMENGEDVLIVYDDLSKHAVAYRTLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARLNENYGGGSLTALPIIETQAGDISAYIPTNV
                     ISITDGQIFLKTDMFNEGIRPSVDSGLSVSRVGSAAQTKAMKQVSGSLKLELAQYREM
                     ESFAKFGSDLDASTKDVLDHGARLTQLLIQKQYSPLTVPEQVLSLYAAKNRYLKPVEV
                     DQVGEFEQGMLEYVHREYPYVLDQIEMKKAIDDELEADIKKALDAYANQYALEKGAQH
                     GTK"
     gene            complement(32004..32531)
                     /gene="atpH"
                     /locus_tag="ACZPDN_00320"
     CDS             complement(32004..32531)
                     /gene="atpH"
                     /locus_tag="ACZPDN_00320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1297103.1"
                     /translation="MVDYTPYAEALFELAQDSKQEIAWMSELKQVAQVITSVSELSQI
                     LAHPSIPRVKKKEIIQTAFESELDTTVFRFLLVLNEHNELSHLDKISDAYEACYRKRH
                     RIEIVKVTSASKLDEDQLSRLKEMLEKKLNKTLELEVQVDPSLIAGIKVQASDLVMDN
                     TLVAKINAMKEAINR"
     gene            complement(32525..33040)
                     /gene="atpF"
                     /locus_tag="ACZPDN_00325"
     CDS             complement(32525..33040)
                     /gene="atpF"
                     /locus_tag="ACZPDN_00325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1297104.1"
                     /translation="MIDFNIDNYLRISLTDVVLVCISTFLIVMFAKKFFWDKLLTLIQ
                     KRQDLIQENIDSSVAIKKQAEDVKEKYDEKLRNVSQEAHTILVSARAHADQEKQQIIK
                     EANDEALRIKKNANEDIERQKRDAQKEMKKAISDVALSAASQLIKKEMDEKTQKQFVE
                     DFIEQAGDKQW"
     gene            complement(33059..33301)
                     /gene="atpE"
                     /locus_tag="ACZPDN_00330"
     CDS             complement(33059..33301)
                     /gene="atpE"
                     /locus_tag="ACZPDN_00330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1297105.1"
                     /translation="MFNEFFVRGMACLGAGIAMIAGLGPGIGQGIAASKGAESVGRNP
                     DAASKIQSIMVLGIALAETTGIYALIIAILLIFIKG"
     gene            complement(33321..34028)
                     /gene="atpB"
                     /locus_tag="ACZPDN_00335"
     CDS             complement(33321..34028)
                     /gene="atpB"
                     /locus_tag="ACZPDN_00335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1297106.1"
                     /translation="MSDVSAIIIHIGKFSLQIQQSILVWLGICVFFAFFFYFAGKKIE
                     AQDPSKSSKGIVYICEEMYNLVLYVIKDNLRETTKKYIPLFGTLIVMMIVSNLIGLIG
                     LQNPTSNVSFNATLAVTMWLMIQGNGIKKGGLLARMKELTEPMFLLTPLNVIGELALP
                     ISLTMRLFGNILAGYIISMLVYTLIKMLMPVGLLGLLVTPFLHMYFDIFSGVIQTYIF
                     FTLSSFFLGQQVAIQED"
     gene            complement(34021..34380)
                     /locus_tag="ACZPDN_00340"
     CDS             complement(34021..34380)
                     /locus_tag="ACZPDN_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297107.1"
                     /translation="MNKQIKKYTLAFSLFCSIACAMIFHSQMIQVFIAVWIGCLTGLV
                     GYHKIVQMALNIPTDEAAGQKMGTQEYMKRYLMYGLVLVVSQLMELPILAVLVGLMCN
                     KGAILLYALKEKEDFHE"
     gene            complement(34394..34561)
                     /locus_tag="ACZPDN_00345"
     CDS             complement(34394..34561)
                     /locus_tag="ACZPDN_00345"
                     /inference="COORDINATES: protein motif:HMM:NF021073.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR1297108.1"
                     /translation="MKPYAFSGMLCTSMLIFGLIGYNIDGWLHTTPLFVIIGLLYSII
                     GSVVLLIKKSR"
     gene            complement(34565..35185)
                     /gene="upp"
                     /locus_tag="ACZPDN_00350"
     CDS             complement(34565..35185)
                     /gene="upp"
                     /locus_tag="ACZPDN_00350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1297109.1"
                     /translation="MLTVIDHPLIKHKLTVMRKKDTGTKDFRQNLDEIGGLMVYEITR
                     DLPLNPVEIETPICPYTGYELAKKVVIVPILRAGLGMVNGIQDLIPTAKIAHVGMYRD
                     EETLEPHTYFEKYPKNLEEAITIVVDPMLATGGSSVAAIDMVKKQGATNIKLVCLVGA
                     PEGVKTVEEAHPDVDIYLASLDDHLNEHGYIVPGLGDAGDRIFGTK"
BASE COUNT        11589 a         7539 c         4910 g        11300 t
ORIGIN      
        1 cgcccacggg tcaccactta tattatgcag gtgtagttca atggtagaac ttcagccttc
       61 caagctgact acgtgagttc gattctcatc acctgctcca tgtaaaatgt ctactcgcaa
      121 ttgcgagttt tttttatcaa aaatgccaag gaactaatcc ttggcatcct taataaatga
      181 atatacatct tcataaatcc tttgattttc tttttcattt aagatttcat gttttaaagt
      241 cggatacaat ttactagata catttttata tccaaccccc atcaaaaatt gttttaactg
      301 ttcaaattta tctttaccct gaatgaccgg atcttcttga cctgcgatca tcaagattgg
      361 caattcacca ttcgtaacct tccaatcctt tttatcaaat gctttaacca tcaattgaaa
      421 taaatttata aaaccatttg ttgtaaataa aaagccacac aattcatccg cttcatactg
      481 atccacattt tctaaatttt tactcaacca acgattaccc gattcatatc ctgcaaaagc
      541 catcttattc aacaggccat tacctttctt ttctttaaag aaaggtttta ttattttagc
      601 caatccaacc gcaaaactag cagccgaatt ttctgtagga ggaccacaca aaatcaagca
      661 acgaatatat tcatcatatt tttttaagta acaacgcgct accaaagtcc ccatactatg
      721 actaaataac gttacctcta atccaggata catatcacgt acgtactcag tcacctgaaa
      781 taaatcatcc acaatataat ccgcattttc cgtatagaaa tatccatagt cattctttga
      841 ttttacacta ccaccatgcc ctctatgatc atgaatcaca caaatatagc ctctttcatt
      901 taaatactgc ataaaatcaa aataacgctc tttatgctcc gccatcccat gtgaaaactg
      961 tacgattcct tttggatttt tgcactccat aatcgcaatc tctaacttta atccatcctg
     1021 ctttgatgta atttttttaa ttatcatatt atagtaccta catttctttc ttgatttcat
     1081 tgtatcaaat atataattat caaaactata caaaaacaaa aaaaagacca ttttttaaat
     1141 ggtccaacta gttttcacta atttctactt ttactttgtg atctgcttcc acatcaaaag
     1201 taatctttgc gaagtcatct aaatgatttg tgacaatatt atgaacttgg tcaaatactt
     1261 cgtcctcttt ttcttcagac ttgcaatcat attcaataga tgtaactgta tgctttttaa
     1321 tttttacatc ttttaattct tctaacattg atttcatagt tatctacctc gatataagtc
     1381 tatcacttat taaatcccgt tgtcaaattt atgcgtgaag ccaatctatt ctatgataat
     1441 aaataaagaa agtaatactt gataaagacc aagtaattgg atagatccaa tatatacacc
     1501 aaatattatg cactaaagca ttcatgattg taagacctgc aacacgcacg aaacaccaac
     1561 aaaccatcat aacaatcatt ggaaccattg actttcctgc ccctcttaaa atagccgaca
     1621 cacaatgtga ataagctaat aaacaaaaga aaaatgcact agtttgtcca cgcaatgtac
     1681 caaactgaat aacttcaggt gtcgcatcaa aggccgcaat aaactgtgaa ccgaacacaa
     1741 aaataatcac accaatcatt tccgccaaag tcatcgaaca aatcatacca aacttagcac
     1801 cctttaaaac acgatcatat tgccttgctc ctaagttttg tccaacaaat gtcgtcagtg
     1861 ccattgtaaa actggtaatt ggtaagaacg caaaaccttc aatctttgaa taagctccac
     1921 atcccgccat agccatatct ccaaaagagt tgatattcga ttgaacaact acattagcta
     1981 gtccaataat ggaattttga aacccactag gcaaaccata ctttaagatt tctcccaaaa
     2041 actccttatg aatccttatt tttgaaagaa caatacgatg tgttccactc gtcgtcaata
     2101 ataaatataa acatagacct gccgaaatat attgcgaaat gattgtagct aaagcagcag
     2161 atcctactcc cattttaaga atggcaataa agaataaatc caatacaata tttacaatac
     2221 tagaaacaat caaataataa agggagtgct tgctatctcc tacagcctgt aaaataccta
     2281 caaaaaaatt atacataatc gatccaaaag aacccaaaaa gtatatttta aaatacgtta
     2341 cagaagcatc cataacactt tctggcgttg acatccagcg aagtaatgta ggcgtaaaaa
     2401 aaatgcctat aagtgtcata aaaaaactta caatcaaccc aaacgctacc gttgtatgaa
     2461 ttgccttctg catacgttct atttctcttg cgccaaaata gcgcgatata actactcctg
     2521 caccaatcgc gactccatta aagaaaccaa tcatcaagaa tataatgttt ccacttgaac
     2581 ttacagccgc aagagcctga cttcccaaaa agtttccaac aatcaatgaa tctaccgtat
     2641 tgtataactg ttgaaataag ttccctaata atataggtaa cgcaaagaaa accatctgct
     2701 taggaatcga tcccgatgtc attaattttt gtccttgtgc caaccctaac acctcctatg
     2761 ttactaaaag aaaaggaagt ttaaacttcc ttgtaaaaac tttgtagatg gctacaagaa
     2821 gaatcaaatt gttctttttc ttcttttgtc atttcaatct caacaacctc tttgacacct
     2881 gtcgcatcca aaatagccgg aacactagtg aatactccat cttgtccata ttgaccatct
     2941 aaataagtag aaaccggaag aactacagat tcattaaaca tcaatgcacg aacaatacgt
     3001 gttgtacttg cggcaatacc ataacaagta tttcctttac gattaaagat ttcccaacca
     3061 tcttttttta cagcttcatg aatactatca aacataccct cattcatacg agttggatta
     3121 tctttaatga tttgataaat atctttacca cccacacgaa ctgtactcca tggaatcatt
     3181 tcactatctc catgttcacc taaaacatac gcatgaatac tctttgtatc tacatcaatg
     3241 caatcggcaa tatgacattg taaacgtgct gaatcaagta atgtacccga tccaataatt
     3301 tgatttgctg gcaaaccaga aactttataa gctacataag tcataatatc cactggattc
     3361 gatacaacaa caataaagcc gttaaagcca ttttccatag cactcgatac aatgctagta
     3421 acaatcttag aacttgattt taaagccact aaacgatcat tttccttacc cattggagcg
     3481 ctagctgtaa tcactaaaac atcagcatct ttacaatctg tataatctcc taattgaatt
     3541 tgcatattac gattcataaa ggcaatacta tgaatcatat ccaaagtttc tcctttggct
     3601 ctttctgtat tcatatcaat taaaacaatt tcatcacaaa tcccttggtt gatcatgcag
     3661 aaagctgtac ttgctcccac atttcccgca ccaacaatta ctacttttct tttcttaatc
     3721 ataatggctc ctcctacaaa aatttagaga tatatccctg tataaatatt accaaaataa
     3781 tcacaggaat gatatatgaa acatagaaac cgacttgttt aggaaaagaa attccttctc
     3841 cacaattggc ttcctttaaa aagtttttaa atccccatcc atatttagat gtacaaaaca
     3901 aaacgtaaca taatgagcct aatggcaata aattattgga tacaatgaaa tcttccaaat
     3961 cttggatggt tgaaccagca cctaatggtt gaataaaaga caacacatta aatcccaatg
     4021 cacaaggtaa agatagcaca aagacaagta caaaacttac ttttatggtt tttgaatgag
     4081 accatccagt ccattccatt gtcatagtta taatattttc gaataccgca ataattgtag
     4141 ttaatgccgc aaagctcaaa aacaagaaga acaaagctcc ccatacattt ccaagctgca
     4201 tattcgcaaa aatattaggt aatgtctgaa aaatcaatcc aggaccttgc ccaggatcta
     4261 cgttatacgc aaaacatgcc ggaatcacaa taaagcctgc aaccaatgcg acaaatgtat
     4321 ctaaacatac aactgtaata gcttccgaag ttaatttttt acttttgtca atatagctgc
     4381 caaatatcgc aatggcacca atacctaaac ttaaggtaaa gaaagcctgg cccatagccg
     4441 caaacacgac ttctgatata ttttgcatct ttgaaaaatc aggcaataag taaaatttta
     4501 cacccgccat tgcccctggc aaaatcagtg aacgtattgc taatataagc atcaacacta
     4561 ataaacacaa catcatatac ttggatactt tttccacacc attctgcaaa ccttttgaac
     4621 aaatgaaaaa gccaatacat acaactagaa acatcattcc tacttgaaca aaagaatttg
     4681 acaacatcaa cccaaactca tcattaattt gacttgcatt caagccattg aaatccccta
     4741 tcaacatctt atagaaataa tagaataacc acccacatac cgatgtataa aacatcatta
     4801 atatgtagtt acctgccata gccacatatt tcataaaatg ccactttgta ccttttggct
     4861 ctaatacatc aaaagaaagt gctgcacttt tttgactggc acgacctacc gaatactcca
     4921 taaccataac tggcaagcct aatatgacaa gaaaaaacaa ataaattaag acaaaggcag
     4981 ctccaccata ttgtccaaca atatatggaa agcgccatac attgccaatt cctattgcgc
     5041 atcccgcgct aattaaaata aatcctaaac gatttgaaaa ccgatctctc atttttccaa
     5101 ctccctataa aaataatcta aacaacctat atatttgcca ctccacggct tcattcttcc
     5161 acaataatga ataatcacac tattttcttt cacccaatct aaatccatct tatttcttgg
     5221 atttcgtaaa ttataaaaat tcatcatcct ttcactcaaa ttatattttc gataatccac
     5281 aagtcttgtc ttatctccat ataatgccgt caatacatcc tgatcaaata acacaagttt
     5341 tttcttatat gcatttacat aattaagcac atcttgttta ttcaattgtt tacgcaacgc
     5401 tattaaattc atcaacaaga ctcctgtatt gatatacggt acatcttctt tcaaccctaa
     5461 tcttttcgca tttaaatgcg tcatattatc actaacatgg ctgcaagcaa tataaagatt
     5521 atcttgaaaa tccatgttat acaattccct taaactttga atcacaacaa tatccacatc
     5581 tagatataga attcgatcta acgtatctgg caacaaatcc gaagcgaata atcgataata
     5641 aatttctaat ggatatcttg atgaagtagg aaaatcctta aaagattctt ccttgacttt
     5701 aataaagtga aaggtgcaat ctggaaaaga ttgcattaaa tcatccttat gctcctgatt
     5761 caaatcttga tgcaaaatat aaaagtctat attcttatca aaacgaaaga tggattgtnn
     5821 nnnnnnnnat tgtatacatg tctttgtcaa acctatgaat tttgcgttaa ttgaaaacaa
     5881 aatgttcata aaatgatttt atcacaaaat aaattgaaaa tcttttcata ttttgtgata
     5941 caatacttac attgaggaga tgatgatatg aaacttatta tacctgaaaa ctacgatcca
     6001 gtattaactg ttagagaaac acaagaagct attaaatata ttcgtgatac ttttcaatac
     6061 gaatttggta aagaattaaa tttatcaaga atttcagcac cattatatgt cacacaaagc
     6121 tctggtttaa atgacaattt aaatggtaca gaaaagccag tatcttttac tatgaaagag
     6181 ttgcctggtg aacaatttga agttgtacat tctttggcaa aatggaaacg aatggcctta
     6241 aagaaatatg gctttaaact tcatgaaggc ttgtacacaa atatgaatgc cattcgtaaa
     6301 gatgaagaat tagataactt tcactctaca tatgtcgatc aatgggactg ggaaaaaatc
     6361 atttctaaaa atgaacgtac ccaacaaaca ctagaagagc atgttcgcac gatttttaaa
     6421 gtcattaaac atatggaaca tgaagtttgg tacaaatatc ctcaggctgt taatcattta
     6481 ccagatgata ttttctttat cacaacacaa gaacttttgt ataagtaccc aaatctaact
     6541 gccaaagaaa gagagaatgc catcacaaaa gaacatggct gcgtatttgt gatgcaaatt
     6601 ggtgataaat tatcaaataa tgaaaaacat gatggtcgcg cacccgacta tgatgactgg
     6661 acattaaatg gagatattct tttctggtat gaaccgctac aaagtgctct tgaaatttct
     6721 tctatgggaa ttcgcgtgga tgaagatact ttattagaac aattgaaaaa agaaaattgt
     6781 ttagaaagat gcgaactgcc atttcataaa gcgattctta ataaagaact accatatact
     6841 ttaggtggag gtattggtca atcaagacta tgtatgcttc tattaaaaaa agcacatatc
     6901 ggcgaagttc aagctagctt atggcctgaa gacatgatcg atacatgctt aaaaaataat
     6961 attcaaattt tgtaggacaa acgtcctact tttttattat tgttccatta gagttggatg
     7021 cataatttcc atatactaaa tcagctaatt cacaaccaga tagtgaaata ttcccatcac
     7081 aaactgtaat tgttccatct atattttcac aatatccatc atatatgaat gcaccatcaa
     7141 ataataaacc atccatgatg atatgtttga tatcaatatc atcacatatt acacttacag
     7201 tacactgatt tttctttgtg ctgtttttta aatatgtata ataggccctt gacaattcaa
     7261 aatatttttc catactatct cctaaagaaa aggaataggc attcacctac tccttaaatc
     7321 ttattcttca gattggatat caattgaacc attgtctgta gtttctgcat cttgtagaat
     7381 tgattcttct actgaagagt ctacagtatc catcatatct ctatcgtcag atcctgatac
     7441 taatttatca aatttttcag aatcttcatt atgaacttca ttcatacgtg caatacgttt
     7501 gtctcgaagt tcttttgcct ttgcagctac gatatcgttt tgaggacgat caccttgaca
     7561 tcctgtacct gcaggaatca atttaccaat gataacattt tctttcaaac caattaagtg
     7621 gtcaacttta cccttgatag tcgcatctgt aagaactttt gtagtttcct ggaatgaagc
     7681 ggcagataag aaagaatctg tttcaacaga agatttagaa atacctagta atacaggttt
     7741 gaaagtagct ggagtttttc cacttaataa tgcttcacga ttaatttttg tgatgttgtt
     7801 aagtgacaat tgaacaccta catttaagtc tgtgtctcca ctttctacaa cgattacttt
     7861 cttcatcatc tgtttgatca ttacttccaa gtgtttatcg ctaatttcga taccttggct
     7921 ttggtatacc ttcttaactt ccttcaagat atagtcttga acttcacgta caccagcaac
     7981 acgtaataat tcttttggat ctaatggacc ttcagtcaac gcaacaccgg cttcgatctt
     8041 agcaccaatc tgaacccatg gacgcattga ttggttcgca ttgattttat gagaaacact
     8101 ttcgtgttca tttgtgataa taacttcttg gcgcattgtt ccttcaatac gttcgattga
     8161 agtgatttca ccagaaatct gagcaattac cgcaacacct tttggacaac gagcttcgaa
     8221 caattcttca acacgaggca aaccttgagt gatatcttct gcaccggcac cggcaacacc
     8281 acctgtatgg aatgtacgca ttgttaactg tgtacctggt tcaccgatag attgggcagc
     8341 cataatacca atagcttcac caacttctac gtctttacca gtagccatgt tacgtccata
     8401 acatttacga caaataccta aacgagactt acaagtaaat acagaacgga tatacatacc
     8461 tgtaacacca gcatctacaa ttttcttagc aagctcatct gtgataaatt catcactcgc
     8521 aataattaat tcaccagttt ttggatctgt tacatcttgt tttgaataac gaccaactaa
     8581 acgatcttgt aatggctcga taacagaatt tgtcttacga tccattaaag tttcgatcca
     8641 gtaaccctta tctgtaccac aatcatcttc tttgatgatt acatcttgtg caacatcaac
     8701 caaacgacga gttaagtaac cagattcggc agtcttcaag gcagtatccg ttagaccttt
     8761 acgcacaccg tgagtagaga taaagaactc acttacgttc aatccttcac ggaaacaaga
     8821 gtaaatagga acctctatga tagttggaac atattcgcca gcacccgctt tagcgtgacc
     8881 cggtttagcc attaaaccac gcataccggc taactgcgta aagtttgacg cgttaccacg
     8941 ggcaccagaa gtcgccatca tgttgattgg gtttttacga ggtaagtttt tcatcaatga
     9001 cgtaacgatc ttatcttttt gatcatccca catcttagat aaatgacgtt cccattcttc
     9061 catagtcaac atacccttac gttgtaagtg ttttaattga tcggctttta cacgaccctc
     9121 atcgatatat tcatctttat gaggagccac ttcaatatcg gacaaggcaa ctgtaatacc
     9181 agctactgtt gaatattcaa atcctaatga tttgatttgg tcaagaattt cagcagtttg
     9241 atctgcatca taacgtttga agacttcggc aataacttta cctaaatctt ttttcttaac
     9301 agcatccacc aaaggcatag cttcgatgta ttctttaatg ttttgtcctg gagaaacaaa
     9361 atatttatca ggagtactta caaagttttc cttactaacc tcattgatat aagggaaatc
     9421 ttcagggaac atagagttga atacaatctt accaactgtt gtaattaaat aatcattttt
     9481 atgtttttca tcaaatgatg ttttatgcat gttacataca ggtaatgcga tacgagtgtg
     9541 aaggtgaact tgttttgact gataagccat cataacttca tcttcatctg tatatacatg
     9601 accttcattt tcaccaaaac ggtgccagtg agttgcttct aaagggcaac caacttcttc
     9661 gtagtgctta gcttctgcga agaattcatc tttcgtttct tccattgtta aatagaagtt
     9721 acccataacc atatcctgtc ctggcgtaac gataggttta ccatctttag gacctaagat
     9781 attgtgagaa cctaacatca taactaatgc ttcggcacga gcttcttctc ctaatggaac
     9841 gtggatggcc atctggtcac catcgaagtc ggcgttgaac gcagtacata ctaatgggtg
     9901 aagacgcatt gcacgtcctt ctactaattt aggcaagaaa gcctgaatac ccaaacggtg
     9961 caatgtaggc gcacggttca tcaatactgg atagccatcg attacttttt caacaacatc
    10021 ccagatacga ggatcacgac gttcgattaa tttttctgca tttttaggat tttgagccaa
    10081 tccttgattt acaatttcat taatgataaa tggtttaaac aagttaactg ccatttcacg
    10141 tggaatacca cattggtaca ttttcaaatc tggtcctacg gcaattactg aacgaccaga
    10201 gaagtcaaca cgtttaccta ataagttttg acggaaacga ccttgtttac ctttcaatga
    10261 gtgagacaat gattttaatg cacgtccacc agcacctgta ataggttttg aacgacgacc
    10321 attgtcaatt aaagcatcta cagattcctg taacatacgt ttttcgtttt gaacgataat
    10381 atttggagta cccaaatcta acaatttacg taaacgatta ttacgagtga ttacacgacg
    10441 atataaatca tttaagtcac ttgtcgcaaa acgaccacca tccaattgaa gcattggacg
    10501 taaatcagga ggtattaccg gaatgttagt cagaatcatc cattctggta agttttcact
    10561 attacggaat gcattgattg tatctaatcg cttgattaat ttcttacgtt tttcaccttg
    10621 cgctttctca agtgcatctt gtacttcttg gtattcagat tctaaatcta catcttgtaa
    10681 tagacgaagt acagcttcag cacctgtttg agcagtaaat cctgttgaac catagatagc
    10741 ttggttttca cggaattcac gttcagaaag aatttgttta taagctaaac cagattcttt
    10801 tggatctgta acaatataac taacaaagta tacaacttct tctaattgtt taggtgttac
    10861 atccaacaac aatgccatac ggctaggaat gccacgtaaa taccaaatat gagctactgg
    10921 ggcagccaaa tggatatggc ccatacgctc acgtcttacg cttgcctttg tgatttctac
    10981 accacaacgg tcacatacaa cacctttaaa acgtactttt ttatattttc cacaagcaca
    11041 ttcccagtcc ttagttggac cgaaaatgcg ttcgcagaat aaaccatcac gttcagcttt
    11101 ttgtgaacga tagttaattg tctcaggctt agttacttca ccatgactcc atttcaaaat
    11161 cgtttctgga ctcgctaagc gaacctgaat cgaggcaaaa ttgttaatac tcatatttta
    11221 tactacgcct cctcttcttc tttattttct tcttcaatat cgtcgactaa ctcttccttt
    11281 tgttcttcct gtaataaagg ttgaactgga tgagcatcct ggtcatcatc atctaatgtg
    11341 atgtcaacct cgttattgtt ttcatctaac aaacgaatat cgattgctag accttgtaat
    11401 tcgtgcatca atacacggaa tgattctgga acacctggtt ctggaatatc tttacctttg
    11461 ataatagctt cataagtctt aatacgacct tgaatatcat cagatttgat tgtcaagatt
    11521 tcttgtaatg tgtgggcagc accatatgct tccaatgccc aaacttccat ctctccaaat
    11581 ctctgaccac cattttgagc tttaccaccc aaaggttgtt gagttactaa agagtatggt
    11641 cctgtagcac gagcatgtaa tttatcgtca accatgtggg ccaacttgat catgtacatt
    11701 acaccaacac taatacgttc atcaaatggt tgtccagttt gtccatcgta aagtacctgt
    11761 tttccatcta cagtcatgct tgcttcgcgc attacatcct gcaagtcttc atttgataaa
    11821 ccatcaaata cagaagtaga gaatttaaca cctaatttac gagctgcata acctaagtgg
    11881 atttctagaa tctgcccaat gttcatacga gaaggtacac cgaatggatt caacaagata
    11941 tccactggat gtccatccgc cataaatggc atatcttcga taggtaaaat cttagagatg
    12001 acacccttgt taccatgacg accagacatt ttatcccctt cagaaatctt acgtttctga
    12061 acgatatata ctttaacacg catatttact ccaggaggaa gatccgatcc atctccacgt
    12121 ttgaatacac gaatagaatg aacaatacca gcaccaccat gaggtacttt caatgaattg
    12181 tcacgaactt cacgtgattt ttcaccaaag attgctaata acaatttttc ttctggacta
    12241 acttcacttt gtccttttgg agtaacctta ccaactaaga tatctccttc ttttacttcg
    12301 gcacctacca taatgatacc gtttgaatct aatttacgaa ccgctgcttc actaacgtta
    12361 ggaatatcac gagtgatttc ttcaggacct aacttcgttt cacgacagtc aatatcatat
    12421 tcttctacgt ggattgaagt atacacatca tcactaacca tacgttcaga catgatgatg
    12481 gcatcctcgt agttatatcc atgccatgtt gtatatgcga taacaacgtt ttggcccaat
    12541 gccaattcac cattttgcat acttggacca tctgctaaga tatccccttt ttcaaccttt
    12601 tctccaactt ctacgattgg acgttggttg atacaagtac tagcgtttga acgagcaaac
    12661 ttacgtaatt gataagtgtg gttttcacct tcattatctt taactacaat acgcaagcca
    12721 tctacatatt caataacacc atcttgttta gcgacaatac caacacctga gtctttggca
    12781 atcttatatt caataccagt accaacataa ggtgaatgag gaactaataa tggaacggcc
    12841 tgacgctgca tgttggcacc cattaaggca cgagaggcat catcgttttc caagaatgga
    12901 acacaggcag tcgctacaga tacaatctgt tttggactga cgtcggcaag ttctacttct
    12961 tcacgatcgg caataattgt ttcaccagcc ttacgagcta cgacacgttc atttactaaa
    13021 tgaccatcac gaacctcatt ggcctgagca attacataat ctgcttcttc atccgcactt
    13081 aagtaaacag tatcttcact aacagtacca tctgcattta ccttacgata aggtgtctgg
    13141 ataaatccat attcattgat acgagcataa gttgttaagt tgttgatcaa accgatgttt
    13201 ggtccttcag gtgtttcgat tggacaaata cgaccatagt gagagttgtg aacgtcacga
    13261 acttcaaaac ttgcacgatc acgactgata ccacctggac ctaaagctga aatacgacgt
    13321 ttatttgtca actcagcaag agggttttgt tgatccataa actgtgataa ctgtgaacta
    13381 gagaagaatt ctttaattgc agctgtcaaa ggacgaatgt tagtcaattt tcttggagtt
    13441 aagctatcca tttcctgaat tgacatacgt tctttaacaa cacgttccat acgagataaa
    13501 ccaatacgga attggttttg gatcaattca ccaacagaac gaatacgacg gttacctaac
    13561 atatcgatat catctgttgt tcctacacct tccataacat ttaagctata tgagaagaat
    13621 gcatacatat ctgaaactgt gattgtatgt ttatttgcga atgggtcatt tccagcaaca
    13681 cgtacttcat ccccattgtt gtcaataata tatacttctt gtacacatgc accaatcaac
    13741 catgccgtaa tatcttcgct taaagcacgt tgtttaactt gtgtttcaac atcactatcg
    13801 atggcatctg gacttacacc aaccatataa cggttcgcaa ctaattccat atctgcatca
    13861 acaatatctt caccttgcgc atttgttaaa cgtccaagag catataaacg ttgaccatag
    13921 ttaaatacag aatccatatt ttcggctgtt aatttaacct gttgtgcaaa cagtccagca
    13981 aaaatttcaa cattttcaac aaccttttgg attgctaaaa catcttgttc agttaatact
    14041 gtaccagcat ctaaataagc atcttccgtt tccacatcac ttgccaatac acgaccaatt
    14101 aatcctgtag tccagcttgt tggaattgaa acgatatctt cttcatggaa ttctgagcgg
    14161 aatggataag cgacacaata agtacctttc gctaattctt cacgcaatgc atttctttca
    14221 tcacggtgaa ccattgtacc tttttccatg aacacattgc catttacatc aacaatatca
    14281 tgagcaaggc tcataccata catacgatcg attgcattta atttcttacg taatttataa
    14341 cgaccagcct ttgtcaagtc ataacgacgg tgatcaaaga atttagcctg catcaaagtg
    14401 attgatccat ctaatgttgg gatttcatca cttctttggt tttcatagaa agaaagtaaa
    14461 gcttcctgtg ttgttttaac tttatcattc aataaagaat tttcgatttc ttcatattta
    14521 ccgaagaagg cagtatacaa caacaaatac atattcatgt attcgcgatc ttctgcatct
    14581 gtagctacat cagaccagtt acgtcccatt gcacgtagga acgtttccat cattgatgta
    14641 tcatgagtat cttcattcac tccaatattc aaagacatac caatagcctt gaataaaata
    14701 ctgaataata ctttacgacg acggtcaata gaaacgttga tcaaacgtcc atttgtagtt
    14761 ttcttttgtt cagtcatgaa ctctaaccaa gtaccacgac ttggaattaa ttcacagcta
    14821 aagttttgtt taccagtctt ttcatcataa ctatctgcaa aatatgcgcc aggagaacgt
    14881 acgatctgac taacgattac acgttctgcc ccattgatga tgaatgtacc agtttctgtc
    14941 attaaaggga aatcccctaa atatacttct tcactcttag ttactacttc cccagtttct
    15001 gagtccgtaa cttctaattc catatctgca tatagaggtg ctgcaaaatt gcattctcta
    15061 tacatagatt cctcagcgtt atattttggt tcttcaaaat gataacgtaa gaaatttaag
    15121 cgaatgtttt ttccatagtt ttcaattgga tagatttctt caaatacttc ttggatacct
    15181 tgttgcgtaa accatttaaa tgaatctgtc tggatttcga caagattagg taactctaat
    15241 ttaccactaa cctttgagta atctcggcgg gaagattttt ttccacacga gacaatacga
    15301 tatgctttgt tgttgtccat gcatgccatc ctttcgttcg aatttgtact atattaggaa
    15361 gttttcattc gaaaacttac actattatgc aatttattat actactagta aaaccaccaa
    15421 atgtcaagaa aaacttgaca aaactataaa aatgggttat aaatattgcc tatccttttt
    15481 ttagcacctt cacaaattag gtgctttttt atttgtccat tttgacaacc catatattta
    15541 tgcatacaat aaagaagtta aaaggagtct ttaatgaaaa aaatattaac tataatctta
    15601 acttgttttt tatcacttaa ccttgcagga tgctcggcct caaaaagcaa cgacgatatc
    15661 cttacattct tcaatgcgtt agataataca ctaaatttaa aaagtgccca aatcaatgga
    15721 acactcgcaa tgaaggattc aaaattaaat attgatgcgc aaattcttca aaaagacgaa
    15781 ttacaagttt ctagttccat cggtcttgtc gcaggaaaaa atgtgcaaaa taactttttg
    15841 aacttttata tcaaagatgg aaaaacatat ttaaattcca tgggaacaaa aacccagagt
    15901 acagtcgata aaattgggtt aaaaaagaat tcaaaattaa atgcatacaa cccatttcta
    15961 gatttaaccg acgatcaatt atgtgaattg tttgattctt ctaaaaaaga aaatgacacc
    16021 tacacattta aagtcaatac aagcaaatta gcaacactac tcgataacat gggatccgtt
    16081 aaactagatc atgctacact tgttgcaacc atcaaaaacg aaaaaatctc acacatgatt
    16141 ttaaacttta ccggaataca aaccgttgat gatgcgagtg ctgatattga tgtttcaatt
    16201 gaactaagta tcaaaaaact aaataagatc aattttccaa acgatttaga caattataaa
    16261 aacgaaactg cagaagatta attcctctgc agtcttttta tttattctcc agcacgtgat
    16321 aattgatcat atgtagatgc aacttgcgta ctaccaatta ttgaaatatt attttcaaaa
    16381 atgacatcat cgattttgtt ttctttacag aaatccacaa tattcatagt taaacatcta
    16441 aagtcagcta tatacacagt cttgtaatga tccaccaaga acggtgcaaa actgttacca
    16501 aacgattctt taatcaataa aaccgactta cctttttcct ttgttggatt tttaatcaca
    16561 ctcaacggat tatctccacc aataaaacaa tagtaaccag aaccttctga ccaatcactt
    16621 acatccgcga ttacgttgta gttagtctca tttccatttt catcccaata taccatatca
    16681 tttgtcgcat gtggaatata cgcatccaca taatctggat tagcctccat ttctggaaga
    16741 cgcaattgat catatgccgt accaaggaat ggttcaaacc tctttgtttc aaaactatct
    16801 aattcttctg gtttccagcc tttttcttta caaaatgctt tgtatgcctg ataagctcct
    16861 aaagcagtcc agtggtgatc tgttctaaaa tatagatatt gatctttggc cttttcaata
    16921 taagaatagt catcaattcc agtcacattc ttataattct tataataata attaatggcc
    16981 tgtttcatat cggaaccacc taattttttc aattcatcat caggcagaac aatcgagtta
    17041 tttggaatca ataatgaata aactttcgat gttcctttta gtcgctttgc aaaacgattc
    17101 atggcattaa tatataaatc tgctgaatcc tgaacaaaat aatacatact atatgcagca
    17161 ccatttttta catacaaatt tgataaaatt tggttttgca tatcctcatc aaatgaataa
    17221 tcttcaggaa ctggaacagg ttctactttt tttgtttttt tcttttttgt aggaatctta
    17281 tccgcctctt tttttgttcc aaccatcata gtcttttggc gaactccata aacatttttc
    17341 atctcttgag ataaattcat caacgaatct ctcattggat atgtatctgc ataccaaatc
    17401 gaaatatcac taaaatattg cccattcaaa aacgttgcca tcgtgaactt tggaaattct
    17461 gttagctttc ttttttctaa atcactcgtc gcaggtctaa caaaaaatgc agcacctgta
    17521 atacaacctg taaacatcaa gcataagcat aaaataacag ctactagttt agtcaaaatt
    17581 ttaaattgtt ttttctgttt catgactacc ccctaaaatt ggaaatacaa gaacggattg
    17641 taactgttcc ccaataaagc caaaaacgaa acaaacatca atgtgaatgg tacaatcgca
    17701 tcgaaaatag aatatagaac cattaccaca ttatctgtat ttgatttctt caccaaatat
    17761 ttatgtaatg attttcctaa agtggtacat gccagcattg agaaaatcaa gaagaaaaca
    17821 ttacttaaga atatagtttt aacttccata ctaaagaatc cagaaacacc aattccaaat
    17881 aatcctaata atacagcccc taattgtgat aaatcagaat atttaaaaat gatccatcca
    17941 aagaatataa cgagcaatgt gtaaacacgt ttccatatat ctggcactcg atcttttata
    18001 taaaattttt ccaataaaag gaaacataga taatataaac cccataaaat gaaattccag
    18061 ctagctccat gccacatccc agttaaaaac cataccacaa acatattacg gatccatttt
    18121 ttcttgctac aacgatttcc gcccaatgga atatatacat agtctctaaa aaatgaactt
    18181 aaagaaatat gccatctacg ccaaaaatct tgaacacttt ttgcgatata tggatagtta
    18241 aagttttcta aataatgaaa gccaatcatc cttcccattc caatggccat atcagaatat
    18301 gctgagaaat ccaaataaat ttgtagcata taacaaatca tagatagcca aattcctaga
    18361 accgattgat tatgaatggc atctaatcct cctggtaata atgtatctgc aatagaagca
    18421 cacgaattgg ctaaaattgc ttttttagct aatccaatac taaatcttcg aaacccataa
    18481 aatacgtctt caaacgagac ttttctgtta tcaatttcat tttgaactgt ttcataacga
    18541 acaataggcc ctgcaataca ttgatgaaat aaactagaat acaataataa cttccaataa
    18601 tcttgttgtg gggatacctc ttttttatat acatcaacaa catatgacaa caattgaaat
    18661 gtataaaacg aaataccaat aggtaaaaca atagttggaa tcttatcagg ccaatgaata
    18721 aaaaacttta agttatttaa aaagaaacct gtatatttga aaatcgccaa caatccaagt
    18781 aaacaacaac acgcaagaat taaattgagc tttgattttt ttgaatcact cattttcaaa
    18841 gcaaaatacc aactaataaa agtttccaac atcaacaata ataaatattt tggtcccccc
    18901 catgcataga aaattagact tgatatcaac aaaaccaaat tcttttttcc attgggtgca
    18961 aaaaaatata caaccagatg tggagtaaaa aatgcgaata caaacaacaa actagaaaat
    19021 accacaatta tctccctttc cccttatagc gctactacta gcactctata ttttaacaca
    19081 aacaaacctt aataattatt aataaatata taaattttta atatcccttt ttcaaaaaat
    19141 acaatataat atattaagat tctgaaagga aaataacatg caaagtatta agacaattta
    19201 taaaatcgga cctggaccta gcagttcaca tacattcggt ccaatgatgg cttctaagta
    19261 tatcaatgaa cagtatcctg atgccacaca tttcaaaatc actttatttg gttccttagc
    19321 acttacagga aaaggacacc ttacagataa aatcatttta aaaacattgg gtgctagtaa
    19381 atgcgaaatc atttttgata tgaaaacagc cgtcgagcat ccaaacacaa tgattattga
    19441 agtatttgac aatgatacaa aggttgcaca acacacattt gtttcaatcg gtggcggtaa
    19501 aattgaaatc gatggtaaaa aaggcaatgt agatccagaa atttatccac acacaaagat
    19561 ggatgatatt caagtctatt gcaacgagcg tcatttacgt ttagaccaat atattgatga
    19621 agtggaagat tctgattttg actcttatat gaatcaaatc tttaaacaaa tgctaaaaac
    19681 agtaaataca ggattaaaga agacaggtgt attacctgga agcttaaaaa ttgaccgagt
    19741 agcccatgca ttataccaat ccgcacaaag ttgtagtgat attcaagata aaaacagttt
    19801 attactaagt agctatgcct atgcagccaa cgaacaaaat gccagtggag gtattgtcgt
    19861 aactgctcca acaatgggta gttgcggcgt tttaccaagt cttgtatatc atttctatca
    19921 cgatcaaaaa tatcctaaaa agacacttat tcaaggttta aaggttgccg gattaattgg
    19981 aaatctaatc cagacaaacg ctactatttc aggagctaaa gctggatgtc aagccgaagt
    20041 tggtgctgcc tgctcaatgg gtgctgcctt tgtcgcttat tgtcaacttc aaaacaacga
    20101 acaaattgaa tgtgctgcag aaattggatt agaacaccat ctaggactca cttgcgatcc
    20161 agttggtgga tacgtaatga ttccttgtat tgaaagaaat gcagtagctg cacaaagaag
    20221 cgtggatgcc gcaagacttg ccaaatattt acgcaacgta aaaaagaatc gtgtaagttt
    20281 tgatatggtt gttcaaacca tgaatctaac aggaaagaaa cttccaattg aactaaaaga
    20341 aagtgcacta ggcggattag ccgttgttgt acctgaaaaa gcataaaggc ctaattcaca
    20401 aatgtgaatt aagccttttt tattacttat cacgaacaaa gcactctaac actttatagc
    20461 cctttcgttc taaatccgct tgtaaatcat ctggttgggt agtatcacaa cgaatgacta
    20521 gattggcaat tccatcttca aaagaataca caccaatatg attgatattc actttgtttt
    20581 caacaaagat ttccgacaac ttaccaatcg caccaatttc atcttttacg gaaaccgtaa
    20641 ttcttgaacc cacttcatcc catccaagaa cattcaagaa acatttaaat acatcatttt
    20701 gcgttacaat acccgtaact tcacccgatg cattgatgac cggcaaacat ccaatattgt
    20761 ttttcaacat tttctgtgtc gcatattcca tcaattcatt ctcatccaca gaaatgactt
    20821 tttttaccat cacagtactt acagttgttt ttgacaataa ataattcaat tcatatatac
    20881 ttaagcttgt cgcattcgtt gtacccgaat ttgaaatcat accctcagta atcaatccaa
    20941 caagttgacc tttttcaaca actggaacac ggtgaattcc cttttccttc atcaaatcaa
    21001 tgatttgtga aatcgttgca tctggttcag cacatataac attttttgtc atataatcct
    21061 ttactttaaa catcttatta tcctcctaga tatgcctttt gaacatccgg actcttggca
    21121 agctcttcac ctgtacctga cattacaact ttacccgtct ctaaaacata ggcacgatgc
    21181 gcaatcgaca aagccatctt tgcgttttgt tctaccaata aaaccgttgt tcccgactta
    21241 ttgatttctt caataatatt aaagatttcc ttaaccaaaa taggagcaag ccccatgctt
    21301 ggctcatcca aaagtaacaa tttaggctta gccattaaag cacgtcccat cgctaacatt
    21361 tgttgctcac caccagataa agtagaagca tcctggttca tacgttcttt taagatagga
    21421 aactgctcac aaaccttttc ataatcatgc ttaattccat ctttatcttt tcttaaaaaa
    21481 gcacccatca tcaagttttc atatacactc atacccgtaa agatacgacg accttctggc
    21541 acttgtgtta aacccatctc aacaatatgt ttcggattgg ccttcataat tgattcattt
    21601 aaaaagttga tttctccacc cgacttttta acaataccac taatggactg catcatcgtc
    21661 gtttttccag caccattagc accaatcaac gcgacaattt cgccttcatt cacctctaaa
    21721 ctaatgcctt ttaaagcttc aataacacca taatgcacat gaagatcttt gatttctaac
    21781 attgccatac tagatctcct ctcctaaata tgccttaact acaaactcat tttgcttaat
    21841 ttcttcaggc gtaccattcg ctatcatttt accatagttc aaaacataaa tacgctcgca
    21901 aatcttcata accaaagaca tatcatgttc aatcaggatt acagttaatt tatatttttc
    21961 tcgaatatga tcaatcaatt ctgttaactc ttttgtttct tgcggattca tacctgcagc
    22021 tggctcatcc aaatataaaa tttttggttg aattgccaaa gcacgagcaa tctccaatcg
    22081 acgttgttta ccgtatggaa gatttgtcgc caattcattt cttaaagaat acaaacccac
    22141 ttcttttaat aattcttctg ccttttcttc gacccacttc tcttctttat aatatgtagg
    22201 caaacgcaaa attgcagcta atgttccata acgaatattt ttatgcatcg caaccttaac
    22261 attttctaaa actgtcattg atttaaataa acgaatattt tgaaatgtac gtgcaacacc
    22321 taagtttgtg attttataag gtttcaatcc accaatctga atactcttat cttgatccat
    22381 caactcaatc aaaccactac ttggttcata gacccctgtc aacaagttga ataatgttgt
    22441 tttaccagca ccatttggac caatcaaacc aataagctct ccctcattga ttgtcataga
    22501 cacattagaa actgcacaaa gaccaccgaa attcttcgtc aaatttttta catttaaaat
    22561 tgttttcatg cagatttctc ctttcgctta tccaataatt tcttaaatga caattctttt
    22621 gtgcctaaaa ttcccgttgg cttaaagatc atgattccaa ccaatgccaa gccataaata
    22681 atcattcgaa tttctgagaa gtttgccaaa acaacattga taataccaat cgcaaccgct
    22741 gctaagacac ttgatgtcat agaacccata ccaccaaata cgaccataac taagatatcg
    22801 attgatttat taaatgtaaa ttgatctggc ttaataacat agaagttaca agcaaacaat
    22861 gctccagcta aagaagctaa taacgcacca atcacaaagg caataacttt atatttcgtg
    22921 gtattgatac ccattgcctc actagcaatc tcatcttcac gaatcgataa acaagctcgt
    22981 ccaggagcag aatgtgtaaa gttacaaaca actagaatgg aaataacagc acacacaaac
    23041 aacaatggaa atgtagttaa ttttggaatt ccatttagtc cggctgctcc atttgtgatt
    23101 tccatattct gtactaggat acgaacaatt tctgcaaacc ccaatgttgc aatcgccaaa
    23161 taatcccctt ttaaacgtaa tgttggaata gaaacaatca aagctacaat ggttgaaaga
    23221 acaaaaccaa tcaatacccc aataaccaaa cctgtcatat tcggcatcat ttttaccata
    23281 ataccagcac aatacgcacc aatacacata aatccagcat gccccaaaga gaattggcca
    23341 catacaccaa taactaaatt taacgaaaca gctaaaataa tattaataaa aatattatat
    23401 aaagtaattg aataataaga tccaataatt ccaactttca ttaaaatcgt taaaactaca
    23461 aaagatacaa cagaaagtaa aagccaagca aaatttgcct tattcaataa ttctttacgc
    23521 atgacttaca ccttctctct cacgttttta cctaataaac cagctggttt taatatcaaa
    23581 atcaaaatta aaataatgta aacaatcgca tccttataca aagaactacc atatgcggta
    23641 accatagttt cagcaattcc aataaataat ccaccaatca tggctccagg aatacttcca
    23701 ataccaccaa atacagcggc aataaaggct tttaaccctg gtgtcatacc cattaatgga
    23761 ttgattgagt tatagtaaat accaactaga acaccggcaa cacctgctaa agcagatcct
    23821 aataagaatg taaaagaaat tgttttatct acatcgatcc ccatcaaacg tgcagcatct
    23881 tcatcgacac ttacagcacg catggcacgg cccatttttg tcttctgaac aataaattgc
    23941 aaaacaacca ttaaaattac ggtaatgaca aaaatataaa cttgttgcat cgtaatcgta
    24001 acgggtccta aatgaaaaga ggcactcgtt aataatttag gataggttct tacttcactt
    24061 cccataatat attgtgttgc atattccaac acataactta caccaatagc tgtaatcaat
    24121 gcggtaattc gagtcgcatg acgaagtggc ttatacgcaa tgcgttcaat caaaacacca
    24181 acgactccac aaaaaaccat tgaaatcaat agggcaggaa taaatcccaa tccacaatat
    24241 gttgttatta gaaatgcaca ataagctcct aacatataga tatctccatg tgcaaagttg
    24301 atcaatttaa taattccata aaccatagta tatcccaagg caatcaaggc ataaatactt
    24361 cctaaggata gaccattgac tagttgttgt aaaaaagcag tcatattaac ctccttatta
    24421 aaaaagaggg ggaattgtcc ccctgaacaa agacttattt atttggatct actgatacgg
    24481 cttcagtttg aacaccatct actaagttaa caactaatac agatttctta ggtgaatgtg
    24541 tcttatcaaa tgtgaatgaa ccagtcaaac cttcaaattt agcatcagcg atagcctttt
    24601 gtagatctgc acctgtttta ccagcttttt ctaaagcttg taaacctaag ttagtagcat
    24661 catatgttaa tgcagagaac atatttggag cttcaccata ttcttcagta tatgcatcta
    24721 caaatgcagt taaatcatca cttgcgttaa ctgtagtata agcagttgaa tagtagcagt
    24781 tatttaaatt ttctttacct gcaagttcaa ctaatttttc tgattcaaat ccatcagcac
    24841 ctaaaattgt gcaatccata ccagcttcac gagcttgttt aataatcaat ccagcttctg
    24901 aataataacc agcgatgaat aatacatctg cccctttatc ttttactttc gtgataatac
    24961 ctgcaaaatc agtatctttc gcattataac tttcagtggc tacaacttct ccacccaaat
    25021 cttcaaattt ttgtgtaaat accttagtaa gaccttttcc ataatcacta acttcattga
    25081 taattacagc ttttctcttt cctaaatttt catatccaaa tttagccatt gccttacctt
    25141 gatatgagtc ttcaaaacaa atacggaacg catattcata aggagaatct ccattcattg
    25201 ttgcatcgac ctgagtagct gatggtgaaa taactggaac ctggttttga tccgctactg
    25261 gatatgttgc gatagaagca ccagaagttg caggacctac cataaaggct acaccatctt
    25321 cagtcatcaa ttttgtagcc gcactagtag attcactcgc atccccttta ttgtccattt
    25381 tgacagcttt aattttatac tttgaatcct tatttgcgtt atattgttta atcgcaagag
    25441 aagccccttt atactccgct tgaccataag aagctacagc acccgataat tcatagttca
    25501 aaccaatttt gactgtatct ccctctttta attctgtact agcagttcca cttcccgaac
    25561 tacatccgac taacatgctt aatgcgacag cactagaagc aaacgttttt aataatttac
    25621 ctttcataat ctatacctcc ctcgagttgt actgatataa taacgcaagc gaaaaacgtt
    25681 ttcaagtgct ttttacattt tattacattt gattttacat tatattttac aatttacatc
    25741 attttcagcc ttttcttaac aaaaaaaata gccaaggcta ttaaacctta gctattcaat
    25801 cacctattaa tacattcctt gaggcattgc aggtgctggt tcttctttat cgatttttgc
    25861 aactccagcc tctgttgtta agaacaaggc tgaaatacta gaagcattca tcaatgcatt
    25921 acgagttacc tttgtaggat caataattcc agactcaatc atatcgaccc acttaccatt
    25981 tttggcatca aatcctacat tttcttcagc tgctttttgt ctttcaacaa tttcttcact
    26041 attatatcca gcattatcag caatctgagt aattggagct gataatgcat caaatacaac
    26101 tttaataccc ttttgaacat cgactacatc tgattttact tcatctttta aagccacata
    26161 agcctttact aaacaagctc caccaccaac tacaatacct tcactaacgg cagcacgagt
    26221 tgagttcaat gcatcttcaa tacgaagttt cttttctttc aattcacttt cagttgcagc
    26281 acccacttta ataatcgcaa caccatttga taatttacct aaacgctctg ccatacgttt
    26341 cttatcgtaa tcacttgttg tgttttccat ttgtgcttca atttcagata cacgagcagc
    26401 cacttcttct ttgcttccag ctccgccgat cattgttgta ttatcttttg tgacaacgat
    26461 cttcttaact gtacccaaat ccgataactg catatctttc aattccatat tcaaatcttt
    26521 agaatagaat ttagcacctg tcataatcgc aatatcctgc aacatttctt tttggttatc
    26581 accaaaacca ggcgcttttg tagcgactac attgaaggtt ccacgaagtt tatttaatgc
    26641 caaagtagaa acaacttcat tttctaaatc ttccgcaata atcaacaatg ctcgatttgc
    26701 ttgaaccaat tgttcaagta atggcaagat ttcctgaata tttgtgattt tttgatccgt
    26761 tactaataca taagcatttt ccattgtagc ttccatcttt tcacgatcac tcaccatata
    26821 tggagaaaca tagcctttgt catattgcat accttcacta atttcaagtt ctgtttcaaa
    26881 gccttttgaa tcatccgttg aaatgacacc atcacgacca accttttcca tcgcatccgc
    26941 aataatctta ccaatttctt cagaacctga tgagatagtc gcaacattgg caatatcctt
    27001 actgctttct actttcttag aaacagataa gatatattca cttacagcct tagctgcctt
    27061 ttcaatacct tcacgcatca atacaggatt tgccccacga tcgacttggc gtaaaccatt
    27121 ttcaatcata ctttgagcta aaattgtagc tgttgtcgtt ccatcaccag ccgtatcgtt
    27181 tgtcttattg gcaacttcat ataccaattt tgctcccata ttttcaaact tatcttccaa
    27241 ttcaatttct ttggcaatac ttacaccatc atttgtgatc aatggtgagc cataatcttt
    27301 ttcaagaaca acattacgtc ccttaggacc taaagtaaca cgaaccgcat tcgccaatgt
    27361 gttgacacct cttaacatag attcgcgagc atcttttgaa aaacgtactt ctttagccat
    27421 aatttattac ctcctactat ttgcttaccg caataatatc ttcatctttg atgacaataa
    27481 attcttcatc atcgatcttc attgtagttc ctgaatattc tttatataaa actttgtctc
    27541 cacaagcata tccagcatct ttcacatctg ctccaatact tgcgacaact gcaagctttg
    27601 acttttcttt tccacttgtc aaaatgattc cacttgccgt agttttttct tctttttctt
    27661 tctttaataa aacataatca tgtaaaggtt gtaacataat tcatcctcct tttagcactt
    27721 aactattcta actgctaaat actattatac tcatttttag cactgatgca ataagtttgc
    27781 taaaatttga caaaagaaaa tggcgaggat tactcgccat atacatgaat tctattaata
    27841 gctctttcta aagataattg agctcttttt agattcgtgt ttgaatcttt cttttctaaa
    27901 cgctctttag cacgttccat tgatttttta gcacgctcaa gatcgatatc ttttttaccc
    27961 tcgatcgcat cagctagaat catgccttca tttttattga attgtaaaaa gcctccagaa
    28021 atcgcataga cttcttcttc gtcattttca ttctttaaaa caagtttaca aggaactaaa
    28081 gatgcaaaga ttggcatgtg atttggtaaa attgtagttt caccgatgac tgtctttgca
    28141 tgaatactct ttacgtcttg ttttaaatat tcgcctttag gtgttacaac tttaaaagca
    28201 atcatgacta ttcaccagcc attttttcag cttttttttg tgcttcttca attgttccaa
    28261 caaacatgaa tgcttgttca ggaagatcat cgaattcacc agctaataaa cgttcaaaac
    28321 tacgaattgt atcttccaaa ggtacatatt ttccaggaat acctgagaat acttcggcta
    28381 cgttaaatgg ttgcgacaag aagttacgaa cacgacgtgc acggtttaca actttcttat
    28441 cttcttcact taattcatcc atacctaaaa tggcgatgat atcttgaaga tctttatatc
    28501 tctgtaggat attttgaacg ccacgagcca ctttatagtg tttttcacca accacaagtg
    28561 gatccaagat acgtgaactt gattcaagtg gatctacggc aggataaata cctaatgccg
    28621 caatatcacg gtccaatact gtttttgcat ccaagtgaga gaatgttgtt gctggagctg
    28681 ggtccgttaa gtcatccgca ggtacataaa tagcctgtac agaagtgata gaaccatctt
    28741 ttgttgatgt aattctttct tgtaactgtc ccatttctgt agctaatgta ggttgatatc
    28801 ctacggcact aggaacacgt cctaataagg cacttacttc agatcctgcc tgtgtaaaac
    28861 ggaagatatt atcaataaac aacaatacat cttgatgttc tacatcacgg aaatattctg
    28921 ccattgtaag acctgttaat ccaacacgca ttctggctcc tggactttca ttcatctgtc
    28981 cataaaccaa taccgtctta tctaatacac cactatcttt catctcgtga tacatatcat
    29041 ttccttcacg agttctttcg cctacaccta ctacaacgga tttaccacca tgctctttgg
    29101 caatattgtg gatcaattcc tgaatcaata cggttttacc aacaccggca ccaccgaaca
    29161 atccaatttt accacccttt gcgtaaggac ataataaatc gattacttta atacccgttt
    29221 ctaatggttc actcgtcgta ctttgttcat caaacgcagg tgcttttcta tgaattggca
    29281 tagtattcgc atctacatca cctaaaccat caattggctt acctaatacg ttaaacatac
    29341 gacccaatac attttctcca acaggagctt gaattggctt tcctgtatca atggcatcca
    29401 taccacgtac taaaccatct gtacttccca tcgcaataca acgtactcga tctgaaccaa
    29461 tatcttgttc gacttctaca attagatttt cttcatttgg gcgttcaatt tcaattgccg
    29521 ttaataaatc tggtaaatta ttttgtgaaa actgaatatc cacaactgga ccaatgatct
    29581 caacgacttt tccaatattt tgactcatct ttaccctcct ttatgaaagt gcatttgcac
    29641 caccgacaat ttcagtaatt tcttgcgtaa ttgcaccctg acgtacacgg ttatatgcaa
    29701 gttcaagatc attctgcaac tcttctgcat tgtcagttgc actctccatt gccattcttc
    29761 gagaagcctg ttcgctagtt ttactctcta aataacgaga gtaaagaatt gattttgtca
    29821 ccataggaat catgttttgt agcattgttt ccttatctgg ttcaaaaatc atatccgcat
    29881 gcatacctga cttcttttct tcctgaggct tagaaacagg taaaaccgtt tctaaagtag
    29941 gaacaaatgt caatgtgttt ttatagtgcg tatataaaat ttgaattttt gaaatttctt
    30001 tcttttcaaa caagtctaat gcatcctgca tacatttcga taaagcatta tatacatcat
    30061 cctcgtcaag atcacttaat acagtttcaa cattatagtt acgttttgaa atccatgcag
    30121 caccacgaac tccaaccata actatatgat cctcatttga aaactcatct tgtatcatac
    30181 gatacacgtt cgcattgtag cctccacaaa gtcccatatc acttgtaatc acaaagataa
    30241 aacttggctt tccttgattc tcatttaaat acatggaatc atttccagca ctacgaagta
    30301 ccattgtgaa aagttcttga agaccttgtg cataactacg gttttcttcc ataatttgtc
    30361 tttgtctagt taatttactt gaagcaatca gttgcattgc ttttgtgatt ttctttgttg
    30421 aactaacgga actgattcga gcctgaatag cctgtctact ttgtgccatg ttgcgcaccc
    30481 ttttctagcg catattgatt tgcgtaggca tctaaagcct tctttatatc agcttctaat
    30541 tcatcgtcaa ttgccttttt catttcaatc tgatctaaaa cataaggata ttcacgatgt
    30601 acatattcca acattccttg ttcaaattca ccaacttggt ctacttcaac aggttttaaa
    30661 tatctatttt ttgcagcata taaagataat acttgttcag gcactgttaa tggtgaatat
    30721 tgtttttgaa tcaataattg agtcaatctt gcaccatgat ccaatacatc ttttgtactt
    30781 gcatctaaat ctgaaccaaa tttcgcaaaa gattccattt cacgatattg agcaagctct
    30841 aattttaatg aaccagatac ctgcttcata gcctttgttt gtgcagcact acctacacgg
    30901 ctaacgctta aaccactatc tacacttgga cgaatacctt cattaaacat atctgtcttt
    30961 aagaagattt gtccatctgt aattgaaatt acatttgtag gaatataagc agaaatatct
    31021 ccggcttgcg tttcaatgat tggtaaagcg gttaaagatc caccaccata attttcattt
    31081 aaacgagcag cacgctctaa taaacgagaa tgcaaataga agacatctcc aggataggct
    31141 tcacgtccag gaggacgacg aagaagtaaa gacagtgtac ggtaagctac ggcgtgtttt
    31201 gataaatcat cataaacaat tagtacatct tctccatttt ccatccattc ttcacccata
    31261 gcacaacctg catatggtgc aatatattgt aatggcgctg cctcagatgc agtagccgct
    31321 acaattgttg tatactccat agctccatgt tgttttaatt tctcaacaat ttgagctaca
    31381 gtactcgctt tttgtccaat cgctacataa atacatttta cattttgacc tttttgattc
    31441 aaaatcgtat caattgcgat ggctgtttta cctgtttgac gatccccaat gatcaactca
    31501 cgctgaccac gtccaattgg aatcatagag tcaataattt ttaaacccgt ttgtaatgga
    31561 acagaaacac tctttcttgt cattacacct ggggcaacac gctcaaccgg acgtgtttta
    31621 tctgtttgga tttcacctaa accatcaatc ggctgcccta atgcattgac aacacgaccc
    31681 atcaaactat caccaactgg aacttcaatg atgtgacctg tacgttttac aacgtcacct
    31741 tctttgatat ctgaattatc acctaaaagt acaacaccga cttgttcatc ttctaagttc
    31801 atgaccatac catatacatc atgagggaat aacaacaatt cactcatcat ggcatttttc
    31861 aatccatata ccgttgcgat accatcacca actgtaagaa tagtaccact ttcattcatt
    31921 tcaagatcct ggtcgatact ttgaatctga gctttgatta attttgaaat ctctgctgga
    31981 tttaaactca tcatatcacc tgcttatcta ttaattgctt ccttcattgc gttaatcttc
    32041 gcaacaaggg tattatccat caccaagtca cttgcctgta ctttaatacc tgcaatcaaa
    32101 gatggatcca cttgcacttc aagttcaagt gttttattta attttttctc taacatctct
    32161 ttcaatcttg ataattgatc ttcatcaagc ttcgatgcac ttgtaacctt aacaatttca
    32221 attctatgtc tctttcgata acacgcttca taagcatcac taattttatc aagatgagaa
    32281 agttcattat gctcatttaa cacaagcaaa aaacgaaata cagttgtatc taattcggat
    32341 tcaaaagctg tctggatgat ttcctttttc tttactcgag gaatacttgg atgtgctaaa
    32401 atttgactca attctgacac tgaagtaatc acttgcgcta cttgttttag ctccgacatc
    32461 caagcaattt cttgttttga atcttgtgct aattcaaata gagcctcggc ataaggcgta
    32521 taatctacca ttgtttatcg cctgcctgtt caataaaatc ttccacgaac tgcttctgtg
    32581 ttttttcatc catttctttt ttgatcaact gacttgcagc acttaaagca acatcactga
    32641 ttgctttttt catttctttt tgagcgtcac gcttttgacg ttcaatatct tcatttgcgt
    32701 ttttcttaat acgcaaagct tcatcatttg cttcttttat aatctgctgt ttttcttgat
    32761 ccgcatgtgc tctagctgaa acaagaatgg tatgagcttc ttgcgaaaca ttacgcaatt
    32821 tttcatcata cttctcttta acatcttcag cctgcttttt aatagctact gaagaatcga
    32881 tattttcttg aattaaatct tgtcgttttt gaattaaagt tagtaattta tcccaaaaga
    32941 attttttggc gaacattaca atcaaaaatg tagaaataca aactaaaaca acatcggtca
    33001 atgaaatacg caaataattg tcaatattaa agtcaatcat taccgattct cccaatcatt
    33061 aacctttgat gaaaatcaat aaaatcgcaa tgatcaacgc atagatacca gttgtttcgg
    33121 ctaaagcgat accaagtacc atgattgatt gaattttact tgctgcatct gggttacgac
    33181 caacactttc tgcaccttta ctagctgcaa taccctgacc aataccagga cctaaaccag
    33241 caatcatcgc aatacctgca cctaagcaag ccatacctcg tacgaaaaat tcattaaaca
    33301 ttttattttc ctcctattta ttaatcttcc tgaatcgcta cttgttgtcc taagaagaac
    33361 gaacttagcg taaagaagat atatgtctga attacacctg agaatatatc aaaatacata
    33421 tgtaagaaag gcgttactaa taaacctaaa agtcctactg gcatcaacat tttaatcaat
    33481 gtatagacca acatagaaat gatatatcct gccaaaatat taccaaataa acgcattgtt
    33541 aatgagatag gcaacgccaa ctctcctatg acattcaatg gtgtcaacaa aaacattggt
    33601 tccgtcaact ctttcattcg cgctaataat ccaccttttt taataccatt accctggatc
    33661 atcaaccaca ttgtaacagc caatgtcgca ttaaaactta cattgctcgt tggattttga
    33721 agtccaatca acccaattaa gttgcttaca atcatcatca caatcaaagt accaaacaat
    33781 ggaatatact tctttgttgt ttcccttaag ttatctttaa ttacgtaaag tactaagttg
    33841 tacatctcct cacaaatata cacaatcccc ttacttgatt tggatggatc ctgtgcctca
    33901 attttcttcc ctgcgaaata aaagaagaac gcaaagaaaa cacaaattcc aagccaaaca
    33961 agaatcgatt gctgaatctg taaagagaac tttcctatgt gaatgataat tgcacttaca
    34021 tcactcatgg aaatcctcct tttcttttag tgcatataaa agaatcgctc ctttattgca
    34081 cattaaccca acaagaacag ccaatatagg caattccatc aattgactaa caactagaac
    34141 tagaccatac attaaatatc gtttcatata ttcttgtgta cccatctttt gtccagccgc
    34201 ttcatccgtt gggatattta aagccatttg aacaatctta tgataaccaa ctaaacctgt
    34261 cagacatcca atccaaaccg ctataaatac ttgaatcatt tgagaatgaa aaatcattgc
    34321 acaagctata gaacaaaata agctaaaagc caaagtatac ttttttattt gtttattcat
    34381 ttgctgcatt gttctacctc gattttttaa tcaataaaac tacactacca attatgctat
    34441 ataataaacc aataatcaca aatagtggag tcgtatgtaa ccaaccatca atattgtaac
    34501 caataaggcc aaatatcaac atacttgtgc atagcattcc cgaaaaagca taaggtttca
    34561 tcaactattt tgtaccgaaa atacgatcgc ctgcatctcc taaaccagga acaatatatc
    34621 catgctcatt taaatgatca tctaatgatg ccaaataaat atctacatct ggatgtgctt
    34681 cttcaaccgt tttcacacct tcaggagctc caaccaaaca aactaattta atatttgtag
    34741 caccttgttt tttaaccata tcaatagccg caacactaga tccacctgtt gccaacattg
    34801 gatccacaac aattgtaatt gcttcttcta aattttttgg atatttttca aaataagtgt
    34861 gtggttctaa agtttcttca tcacgataca taccaacatg tgcaatttta gcggttggaa
    34921 ttaaatcttg aattccattt accataccaa gtcccgcacg taaaattggt acaatgacca
    34981 cttttttagc caattcatag ccagtgtacg gacaaattgg agtttcaatc tctactggat
    35041 ttaatggtaa atcacgtgtg atttcataaa ccatcaaacc tccaatttca tctaagtttt
    35101 gacgaaaatc tttcgtcccc gtatcctttt ttcgcataac tgttagttta tgcttaatta
    35161 aaggatgatc aataactgtt aacatttaaa ttacctacct ttatagccat aattataaca
    35221 tccttatcca taatagaaaa cactgaaaga aagggttttc gccaaatctt ccagtgctta
    35281 attatataga tattcaattt tttaatcagt aaacaattct actcaattgt caaaatttca
    35341 aactgttt
//
LOCUS       DBMDWL010000003        28226 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_648_length_28226_cov_2.751751, whole genome shotgun sequence.
ACCESSION   DBMDWL010000003 DBMDWL010000000
VERSION     DBMDWL010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 28226)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 28226)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28226
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_648_length_28226_cov_2.751751"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2470)
                     /locus_tag="ACZPDN_00355"
     CDS             complement(<1..2470)
                     /locus_tag="ACZPDN_00355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1297110.1"
                     /translation="MVHLHMRSSYSLLESPIRLESMILHQKQLGYNHVCLTDHNVMYG
                     TMEFYQLCKKHNVHAIIGLEVDSIYNEEPFHFILLAKNDMGLQNLYKLSSIIKENVSF
                     DEVIKYAKDCVVLTSSDGKDTFSAYIEHQDLEKLSAFVELCKNSFSDFYVSISMNDSG
                     KKRVDNKILKQLDCKCVALSRILYEKSEDVKSLQALRAIAKQTSIENQGLDVQFQRYL
                     RSPQEMEALYDERDLLATEQIARMCNVQMAFQKSKLPVFENKLHIDSSDYLVKLCKKG
                     LEKRLNGNLDAVYMNRLEYELSVIINMGFTDYFLIVWDFIRYARSKDIFVGPGRGSAA
                     GSLVAYCLGITHIDPIQNNLLFERFLNPSRISMPDIDTDFPDDRRDEVIRYVRKLYGQ
                     DHVSHIVTFNTLQARQAIRDVGRVMNIPARIVDELSKMLKNTPKITLMQAYNENALFR
                     KRIQSDQKLIELFQMCVSIEGLPRHTSLHAAGIVLSDQPIVNVCPLVSVDGDIQATQF
                     TAEYLEDLGLIKMDFLGIRNLTTIHEIVTNLKEQKHISLDMMKINLKDAKTYQLLSLG
                     NTLGVFQLESSGITSLLQKIQPNKFEDISVVLALYRPGAMQHIDTYIERKRDPSKVVY
                     PHPLLEPILKETYGIMIYQEQVMQTAQVIGGFSLAQADTLRKAMSKKKLDVMQNLKEQ
                     FILGARKNRIKDSVSNEIFSIMEQFAGYGFNKSHSYAYSLVAYQMAYLKANYPLYFYQ
                     SLLNSVIGSGIKTSQYIYECKRRNIVVNGCDINHSKAFYTIENNSIRMPLQVLKGVGK
                     TIYPTIIKHVPYTDLFDFLAKASMY"
     gene            complement(2525..3457)
                     /locus_tag="ACZPDN_00360"
     CDS             complement(2525..3457)
                     /locus_tag="ACZPDN_00360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1297111.1"
                     /translation="MPNAILESVKDFNTITIFRHVFADMDAIGSQFGLKYYLESAYPD
                     KKIYCLGSNCPVSQRNNVTMDEVDDDVVASSLAIVLDTSNAARIDDERYKFAKKSIRI
                     DHHVQVETICDEEWIDDKASATCELLALYLQENKVNIPVESALMLYLGLTADNIRFTT
                     NNVRPATFDAAKYLFEQGVDVTKVEQLNFSKSIEDYRYETVVRNHTILKNKFLYSILE
                     CEDYEEYGLNFTDAKDKVYCLAGIDCVEMWALFTRMEDRIHYSASLRSRNIPIRDVAM
                     EFGGGGHECASGIKNLTRDQVDQIIEILSNREVA"
     gene            complement(3450..4649)
                     /locus_tag="ACZPDN_00365"
     CDS             complement(3450..4649)
                     /locus_tag="ACZPDN_00365"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR1297112.1"
                     /translation="MSIVMSVNAGSSSLKFQVFKMPEEEVLAQGNVERIGLNDSIFGM
                     KANGEKIEEILDIKDHAVAVKKLMEALIEHKVVNSLEDIKAIGHRVVHGGEYFSHSVP
                     VDADVEAKVEELCALAPLHNPAALVGYRSFRDALPECKHTFVFDTAFHQSMPEENYIF
                     PLPYEYYTNDKVRRYGAHGTSHEYLTKRLAELQGKTQEEMNIITCHLGNGASITAVKN
                     GKSYKTSMGFTPLGGIMMGTRCGDIDPAIVPFLENKYGYTPDEMDTIMNKKSGMLGVS
                     GISSDGRDIEAAVKEGNPRAILTQNVYVNRIVEVVSGYYGLMGGADAIVFAGGIGEND
                     CAMRQRICDGLSAFGVVVKPEDNDGVRGVEKLLSAPESKMQVWLLPTNEELVIARDAY
                     KDLTANA"
     gene            complement(4662..4955)
                     /gene="ylxM"
                     /locus_tag="ACZPDN_00370"
     CDS             complement(4662..4955)
                     /gene="ylxM"
                     /locus_tag="ACZPDN_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016205.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR1297113.1"
                     /translation="MISKLDANDLFDVYGDLLTKRQREILSLYYEEDFSYTEISEELE
                     ISRAAAMDSVHRATKLLEKYEAAIGYVAKKKLLDAWILQHEDVEQIEILKEIF"
     gene            complement(4955..5914)
                     /gene="ftsY"
                     /locus_tag="ACZPDN_00375"
     CDS             complement(4955..5914)
                     /gene="ftsY"
                     /locus_tag="ACZPDN_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1297114.1"
                     /translation="MSVFSKIKEAFVKSEDKDKYLSGLDRSKKSFGDRMRALSNNFHG
                     IDDDLLEQIMVILLESDVGIHTAQKIVDAFESNGKHIKDYDSMLDYLISILYDFYDES
                     SDEPIKMNEEGPTVILMVGVNGSGKTTTSAKLIQYYKEQGKKVAVAAADTFRAGAIDQ
                     IDTWANRLGVPCIKGKMNQDPSSVLVDACRYAKEKDVDILICDTAGRLQNKTNLMNEL
                     SKMHRVVQREIEGAPQEVWLVLDATTGQNGISQAKVFLEATDVTGIVLTKMDGTAKGG
                     VVLAIRDLLHLPVRFLGLGEKPADLRPFDINAFLMGITKGIEE"
     gene            complement(5914..8841)
                     /locus_tag="ACZPDN_00380"
     CDS             complement(5914..8841)
                     /locus_tag="ACZPDN_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1297115.1"
                     /translation="MFLKRIELQGFKSFADKTVIQFDQDITGIVGPNGCGKSNVNDAI
                     RWVLGEQSVKSLRSGTNMSDIIFSGSEYRKPVNMARVTLVFDNSTRVFDSDFDEIEIT
                     RQILRANNEASYFINKTPCRLKDINDLVMDTGLGKDSLSIITQGNISSFADAKPEDRR
                     SLFEEAAGVAKYKKRKKVSLSKLEQTKENLDRLQDILDELERQIGPLEKQAKKAEKYI
                     SLREKLSKIEISVLVEDIDQYNEKINQINKELFDIQAMHTSENAELLKQETRLESIRK
                     EMYALDKQINELQGKYTKAMEENYQLERRKIEQDEKRKYMLKVADKKARQKEIQAMLE
                     EARFEYQDRHQRLMQTQQDLNNRRSIVNDLKMKISKARYESDQANNILTQLQNRRQVL
                     ENMMKQPFAHQQGVRSVMQAKNSLSGVYGVVSELLIAHADKALAVNAALGGSIYQIIT
                     KNEADARNAISFLKRNRSGRATFLPLSVCHPRKMNEQVITIASTSPGFLGFASECVDC
                     KEIFDPVKERLLGNVIVVDTLQNANETAKRLRYAYKIVTLDGDIVHTGGSMTGGVTKN
                     QSTPVTMRQELDTINSKIEGQKIKADSCLNETEILTQKLQKEKDAIVTLQIELAKLEN
                     IYATKKAKYDSILAEYQELGVDIEENAELAQDDLVVQMSKMHAVLDSLSLEIQSLRQS
                     RFDKGNDAEQLENQIRLVRREMNSKQSQIHNYEMEIVKVKTQLENALNRLSTDYEMTY
                     EYALTKKEDLEIESAKEEVIQLRQAISRLGNVNLDAPNEYKEVKERFDFMTSQKEDLE
                     KASQQILAAIDEMDKTMISQFTDMFNKINAELDGVFKAMFGGGRASLSMVDPDDVLNT
                     GIDIDVQPPGKMVKNIQTFSGGEKALIAISVLFAILKARTMPLCIFDEVEAALDQANV
                     ERFARYLSHYRGQSQFIAVTHRPGTMEQCDTLYGVTMQKDGVSKVLKVQLKDAVHIAK
                     EEE"
     gene            8950..9606
                     /gene="trhA"
                     /locus_tag="ACZPDN_00385"
     CDS             8950..9606
                     /gene="trhA"
                     /locus_tag="ACZPDN_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR1297116.1"
                     /translation="MNKKDSMRYVYPLCFGEEVGNCITHGVMAILILFSLPYYSVRAY
                     LRGGTIETVGTAVFLICLLFMFAGSCLYHSMPHETTHKYVFRKLDHIMILLAIAGTYT
                     PICLKLMNNWKGYTILAIEWVMVIMGILLKSIATKTYPKLSMTIYMVMGWLAIFILPT
                     LIKKTQPIFIILIALGGIMYTIGTFFYSKPQKRYFHFVWHIFIILASICHLLAILYFI
                     "
     gene            complement(9644..10717)
                     /gene="rseP"
                     /locus_tag="ACZPDN_00390"
     CDS             complement(9644..10717)
                     /gene="rseP"
                     /locus_tag="ACZPDN_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR1297117.1"
                     /translation="MDFVIGLIAFIIMLSVIVILHELGHFLVAKHFGVYCKEFSIGMG
                     PCLYQKQGKETAFSIRAIPFGGYVMMAGEEDGSQSEEDSWLKDIPENRRLNGIEKWKQ
                     VCIMIAGIVMNILLAWIIYMGVALVQGYVVEEAKPVVYVVEENSVAQKAGLEKDDHII
                     KVLSEDGNSIQPKTQYEILEFIQYHHDTLTLTVKRDKTTFKTTLTPSYDKDMEGYTLG
                     YKAIAYAKKIPWYQSLWVGCQNTWDSATTIFKSLNMIIRGQGLENLSGPVGILNVTSK
                     SVQYGLDMYFSLFAMISLNIGIFNALPIPALDGGRILILLIEKLIGRKVSTKIVENII
                     LASFVLLMILFIYATYNDIARMF"
     gene            complement(10729..11496)
                     /locus_tag="ACZPDN_00395"
     CDS             complement(10729..11496)
                     /locus_tag="ACZPDN_00395"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1297118.1"
                     /translation="MKQRIITAIGIILVVALPVALGGLWLEALALFIVCAGAYEWMHI
                     QPDFKKWPKYVMPLSALAVIFTRILPDKYFLVVITLIVLFLWSLVVFVENFSIMDSFV
                     CISYVTIFSLVYHAVGQISGVHQYLWTIVFATYGSDTGAYFVGRAIGKHKMNPRISPK
                     KSWEGFVGGIVFGFILSLVVSFSYVSNLNPVLNTLLCLLCPITAELGDLCFSAIKRHF
                     AVKDFSNLLPGHGGVLDRVDSLLLNIMLFSVLYTIIL"
     gene            complement(11493..12185)
                     /locus_tag="ACZPDN_00400"
     CDS             complement(11493..12185)
                     /locus_tag="ACZPDN_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR1297119.1"
                     /translation="MAPKTVAIIMDGNGRWAKKRGLPRTAGHKKGADTIRTVALAAQD
                     MGIQKLILYAFSTENWKRPEDEVSYLCKLPGLFFNKFINELMEKNIQVTFAGELEKFP
                     KATQDVINKAVNMTSKNTGLELCLAINYGSRREMLLSIKKYADEVASGKRENDLTEEE
                     FSKYLFVPEDIDLMIRTSGELRISNYLLWQLAYAELIFTPVAWPDFDEKALKDCLDEF
                     ASRDRRFGGLTS"
     gene            complement(12226..12771)
                     /gene="frr"
                     /locus_tag="ACZPDN_00405"
     CDS             complement(12226..12771)
                     /gene="frr"
                     /locus_tag="ACZPDN_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR1297120.1"
                     /translation="MSDILLNAEEKMMGAIDNLESNLRQIRTGRASGQMLERVQVEYY
                     GAMTPINQIAQISVVEGTQLVVKPYDRSIVKGISHAIQATNLGLNPQAEADVIRIQVP
                     QLTEDRRKELAKDAQKYGEEAKVAIRNVRRDANDAIKKDKELPEDEKKQTLEESQKLT
                     DKYIKDVDSITETKKKEILNV"
     gene            complement(12783..13493)
                     /gene="pyrH"
                     /locus_tag="ACZPDN_00410"
     CDS             complement(12783..13493)
                     /gene="pyrH"
                     /locus_tag="ACZPDN_00410"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR1297121.1"
                     /translation="MYKRVLLKLSGEALSSPEAAFDPEILKRLAKELKEVQKLGVELA
                     IVVGGGNFIRGKMMAEMGMNRAQADYMGMLGTVINALAVQNALEQEGVQTRVQTAVEM
                     QKVAEPFILRRALRHLEKGRIVIFGAGTGSPYFSTDTTAALRASEINADVILMAKNGV
                     DGVYDSDPKKNPNAKKYDTLNYMDVLNEGLQVMDSTAISMCMDNDIDLVVFNMNEPGN
                     IVKAVRREVNGTTITKKK"
     gene            complement(13596..14486)
                     /gene="tsf"
                     /locus_tag="ACZPDN_00415"
     CDS             complement(13596..14486)
                     /gene="tsf"
                     /locus_tag="ACZPDN_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR1297122.1"
                     /translation="MAITAAQVKELREKTGAGMMDCKKALTECEGDIAKAVDWLREKG
                     IAKSAKKEGRIAAEGLTRVAVSGNTAVLFEVNSETDFVSKNEQFLGLMNTLQEAILAN
                     KPASTEEALNIQTAEGTINDLIINATATIGEKISFRRVAVVEKADDEIFGSYMHMGGS
                     ISAVVVVKGTEDATVAKNLAMQVASMAPKYVSQAEVPSEEVEHERELQLQMMKADPNM
                     AKKPEKVLVGILKGKVDKHFKDQCLLDQEYFLEPKTKVSQFLKDNKAEVVTFVRFQTG
                     EGIEKREENFAEEVAAQMAK"
     gene            complement(14511..15416)
                     /gene="rpsB"
                     /locus_tag="ACZPDN_00420"
     CDS             complement(14511..15416)
                     /gene="rpsB"
                     /locus_tag="ACZPDN_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR1297123.1"
                     /translation="MSIVSMKKMLENGVHFGHQTRRWNPKMKPFIYTSRNGVYIVDLN
                     QTQAQIEAAYNELKAISERGGKVLFVGTKKQAAEVVEEQALRSGSFYVNKRWLGGLLT
                     NFRTIQKRIKRLVEIEEMEASGKIEVYTKKEQANLRKEKAKLEGSLGGIKEMKKLPDA
                     LVVIDPKEEHNAIAEAKKLGIPVFAMLDTNCDPEDADYPIASNDDAVRSIKLVTTILA
                     DAIVDAKGGLLEMAYLDETEDDVTMEDVIKNVEAQIAENERRRRQRNEERRKRNFERK
                     NRRPFNKKGDDVKPAAKAEEAKAAE"
     gene            complement(15561..16436)
                     /gene="xerA"
                     /locus_tag="ACZPDN_00425"
     CDS             complement(15561..16436)
                     /gene="xerA"
                     /locus_tag="ACZPDN_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HIR1297124.1"
                     /translation="MDELIERFLKYIYRKNTQSEKTIESYKNDLNQFKEYLTSQSIDS
                     FESCDRLTFMNFLATLDHLKASSIARKMSVYRSFYTYLAEYMGIIENPLIGIQTPKKS
                     KQIPDFLFVEEIQEFLNSYNDEKDDEYRDRILFTIMYACGLRVSECVNLEWKQIDLNN
                     RIVHIRGKGDKDRIVPFYQGFEKELLEYKSKFWTKYAIDDHVFVNLKGKSLTSRGIQY
                     LMDKHAKKIGMHMKLHPHMLRHSFATHLLDNGADIRVVQELLGHSSLSTTQIYTHVTT
                     AQLVKAYKKAHPFEK"
     gene            complement(16429..17727)
                     /gene="trmFO"
                     /locus_tag="ACZPDN_00430"
     CDS             complement(16429..17727)
                     /gene="trmFO"
                     /locus_tag="ACZPDN_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR1297125.1"
                     /translation="MERVTVIGAGLAGCEATWQLIKRNIPVRLVEMRPKKESPAFHTD
                     RFAELVCSNSLRSNAMNNAVGILKEELRQMDSLIMKSADMHAVPAGSALAVDRETFSQ
                     YITDIIKNHPLVEVVNEEMTSLPEGPCIIATGPLTSDTLAKAIHDFTAEDTFHFYDAA
                     APIIEKDSIDFSKAYYKSRYDKGEASYINCPMNADEFEVFYDALIHAECVNLHDFEKE
                     TYFEGCMPIEEMARRGKKTMLFGPLKPVGLEKDKDSHPVAVVQLRQDNVAASMYNIVG
                     FQTHLTWPEQRRVFALIPGLENLKITRYGVMHRNSYLSSPVVLKETYQSKKRDDLFFA
                     GQLTGVEGYVESCASGLIAGINMALYIQGKEPICFGNTCVMGSQAYYITHCDAKHFQP
                     MNANFGIMHLNEKCKKHERKEKFASQALARIAQIKDEVDG"
     gene            complement(17727..19859)
                     /gene="topA"
                     /locus_tag="ACZPDN_00435"
     CDS             complement(17727..19859)
                     /gene="topA"
                     /locus_tag="ACZPDN_00435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1297126.1"
                     /translation="MKKHLVIVESPSKSKTIEKYLGKDYRVVSSKGHVCDLATSGKEG
                     LGIDVEHDFEPKYKISKEKKEVVKELKEYASKAKDIYLASDPDREGEAIAWHLARVLD
                     LNIEDNNRIVFHEITKPAILEAMKEPRQIDMDLVKSQETRRMLDRIIGFKLSKLLQNK
                     IQSKSAGRVQSVALKLIVDRENEIKAFKQEEYWTIHAQCKKQNKAFEADLVKVEGKKP
                     KIKNEEEAKALLERCNGEYVVSSISKKQKKKQPKLPFTTSTMQQEASTKLGFGAKKTM
                     SVAQKMYEGIDLGGNMEGLITYMRSDSTRLSDIFVSDAKEFITNTYGKEYVGHVHQKN
                     GKNTQDAHEAIRPTNINRTPESIKDHLTNDQYRLYSLIYARTLASLMKDAVYDVTSIK
                     ITNNDLEFSASGQTMTFDGYYKVYSKYEKQSDALLPTLEENEVLKNVTPISKQHFTEP
                     PARYTEAKLIKDLEEKSIGRPSTYATIIDTLQKRGYASLDKASETSKTKVFFPSEQGI
                     LTNEKLQQYFSSVINVEYTADMEKTLDEIAEGNEDYISYMHKFYDKFAPLVEDAYEKM
                     PKKELERVGRTCPECGGELVYRNGRFGKFISCINFPTCRYTENNEEELPEEAKEEKIC
                     PNCGAKMVIKRGRYGQFWACSNYPECKTIESLKKKAKPEPTGEMCPECGHELVRRKSR
                     FGTTFIGCSNYPKCHYIKKEPKKKEEEK"
     gene            complement(19915..20646)
                     /gene="dprA"
                     /locus_tag="ACZPDN_00440"
     CDS             complement(19915..20646)
                     /gene="dprA"
                     /locus_tag="ACZPDN_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1297127.1"
                     /translation="MKINRQAILWYAIQYDGDWKKIGNAIKAHENYSIIDYPYAYLTI
                     MDDAYPDAFKRLRYPPWIIFYQGDINLLKEKGVGIVGARNCSTQALQNTDSIVQRLQS
                     KYVIVSGLAKGIDARAHYNATKTIGVLGCGINVIYPKENTYLFERMKNNGLIISEYPM
                     HVKPLAYHFPWRNRLIAALSDSLVVIEAAHKSGTMITVNECLELSVPIYCVPTAFQDE
                     KHQGCNYLISNGANILVDIKDVDDI"
     gene            complement(20689..21297)
                     /locus_tag="ACZPDN_00445"
     CDS             complement(20689..21297)
                     /locus_tag="ACZPDN_00445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1297128.1"
                     /translation="MLDRPLEHDYWDQNQSVLGMDEAGRGPIAGPLMVAGVIFPKGYQ
                     NALIDDSKKLSEKKREALFKTIIQDALYYQILVVDEKTIDKKNIYRATQEAMMEIANK
                     AQCDFVLSDAMPLPDIDKPFQAIVKGDQKSLSIAAASILAKVTRDRWMKKLDEMYPQY
                     GLAKHKGYPTKAHIEALNQYGLQDFYRRSYGPVKKLEEIRLF"
     gene            complement(21290..22135)
                     /gene="ylqF"
                     /locus_tag="ACZPDN_00450"
     CDS             complement(21290..22135)
                     /gene="ylqF"
                     /locus_tag="ACZPDN_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR1297129.1"
                     /translation="MANVQWFPGHMTKAKRQMEENLKKVDFVIEIRDARMPDASKNPM
                     LDQLIQNKPRLILLSKKDKADKELTKQWVSALETENQKVLALDFIHENFSKQLIKASN
                     ELCINLIEKQKRRGMKPRALRAMVCGIPNVGKSTFINTFAKRKAAQTGDRPGVTKSLQ
                     WIKVGQTLELLDTPGVLWPKFEDQQVGLKLALLGSIKDQVVNMDDLALFACEYLMSEK
                     PEIFDSVYHVEIANTPYETLQNIAIKRGYLLKGEIDENRLMLSFVKDIRENKCGLITW
                     EKPHA"
     gene            complement(22135..22764)
                     /gene="lepB"
                     /locus_tag="ACZPDN_00455"
     CDS             complement(22135..22764)
                     /gene="lepB"
                     /locus_tag="ACZPDN_00455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1297130.1"
                     /translation="MKKAKKSELRYNEDDERTLLEDILGFIKVFVVSAIVILLFVNFV
                     AHPVRVDGRSMYPTLKDGEFGFTNVGGVLLNGVKRGDIVVVTMEEKGQKTHWVKRVIG
                     MPGDTISCVNDIVFINGKVLDETQYIDPDYRQSCVDKFGYFNKVPNSDNTDVQDFEEV
                     KLGDDEYYVMGDNRPYSKDSRYVGPVKKSQLFAKKMLVLLPISDIGVKD"
     gene            complement(22848..23195)
                     /gene="rplS"
                     /locus_tag="ACZPDN_00460"
     CDS             complement(22848..23195)
                     /gene="rplS"
                     /locus_tag="ACZPDN_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR1297131.1"
                     /translation="MNLALVNEVTKSQLKTDIPAFRSGSTVRVHVKIKEGDKSRIQVF
                     EGVVIERAGGGISETFTVRKISNQIGVERKFPLHSPIIDHIEVVRHGKVRRNKLSYLR
                     NRSGKAARLKEVR"
     gene            complement(23314..24036)
                     /gene="trmD"
                     /locus_tag="ACZPDN_00465"
     CDS             complement(23314..24036)
                     /gene="trmD"
                     /locus_tag="ACZPDN_00465"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1297132.1"
                     /translation="MKISVLTLFPEYFEPLMTTSILKRAKEKDLFEFETIDFRKFTKE
                     KHGHVDDTPYGGGAGMVLMCQPILDALESIRTENSTVILLTPQGKTFNQQIAKELSLK
                     EHLIFICGHYEGFDERIRDYVDIQMSLGDFVLTGGESACMVMCDCILRILPGVIRQDS
                     SDDDSFSNGLLEYPQYTRPEVYNGKRVPDVLLSGHHANIKKYRHEESLKRTALHRPDL
                     LENLELDKEDQKVVDTVLKNKN"
     gene            complement(24033..24521)
                     /gene="rimM"
                     /locus_tag="ACZPDN_00470"
     CDS             complement(24033..24521)
                     /gene="rimM"
                     /locus_tag="ACZPDN_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865917.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1297133.1"
                     /translation="MIKVAQIINTHGLKGECKLYLVTDDPQHRFEKGRVLHLEDGRTL
                     TVVRYREQKGFGYCYFEGIDSIEKAEQLKTKNLFIAQDDLPELEEGQYYYHELMNCTV
                     YNEEKENLGEVVDILETGANLVLRVKSKNTSFLLPFVPAFIVDVNKDTHEIIIREMDG
                     LR"
     gene            complement(24559..24807)
                     /locus_tag="ACZPDN_00475"
     CDS             complement(24559..24807)
                     /locus_tag="ACZPDN_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1297134.1"
                     /translation="MIDLEKTLLDICLPLVDDQKALSVKTMATTNENEILLYVYANSE
                     DVARLIGRKGIMASSIRQMMSIAARDVHKRISVKFEAY"
     gene            complement(24807..25076)
                     /gene="rpsP"
                     /locus_tag="ACZPDN_00480"
     CDS             complement(24807..25076)
                     /gene="rpsP"
                     /locus_tag="ACZPDN_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1297135.1"
                     /translation="MVKLRLKRMGKKGAPFYRIVAADSRSPRDGRFIEQIGTYNPSKA
                     ENKVTLDKELTKKWLSNGAQPTDTVRSILSHEGVLKEMHEAKSSK"
     gene            complement(25173..26591)
                     /gene="ffh"
                     /locus_tag="ACZPDN_00485"
     CDS             complement(25173..26591)
                     /gene="ffh"
                     /locus_tag="ACZPDN_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1297136.1"
                     /translation="MAFESLSERLNKAFKNISGQGKLTEKNMTDMLKEVRMSLLEADV
                     NYDVVKSFVENVKEKALGQEVLDSLNPSQMVVKIVHDELQVLLGEEDACIHFKKQGMT
                     TVMMVGLQGTGKTTASAKIANYCKNKLARRVLLVACDVVRPAAIEQLQTLGKSIDVEV
                     FTCGPETSAVETAKQALAYAKDRQKDLVIFDTAGRLHIDEALMQELQQMKELVVPDEI
                     LLTVDAMTGQDIVTVAKEFNEQLAVTGLIVTKMDGDARGGGLLSVRSITNVPVLFVSN
                     GEKVDDLEEFHPDRMADRILGMGDIVTLVEQAQDKLDIEVQKKTAERMKQGVFTFNDL
                     LAQLGQVSKMGSMQKMMNMIPGINKVAKNLDDEKMNSQMKKSEAIILSMTEYERENPS
                     CLKASRKNRIAKGAGVKVMDVNRLINQLEQMQMMTKMMSGKVKLPDMAGLQNMQRGGG
                     MPGMSKHTGSKKQKAKKKKKKK"
     gene            complement(26625..27128)
                     /locus_tag="ACZPDN_00490"
     CDS             complement(26625..27128)
                     /locus_tag="ACZPDN_00490"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1297137.1"
                     /translation="MARELIRRATLEDMPILLEIYDRARKFMASCGNVNQWTNGYPSK
                     EILSEDIAKGEMYVLEDEDGIQASFVFYCGIDPCYNKIYDGQWLNDEPYGVIHRIATR
                     GLKKHMANIILEYCFSKIDNIRMDTHTDNKPMQNFMIKHGFKHVGTIYLKNGQSRLAF
                     HCNLNEN"
     gene            complement(27113..28045)
                     /locus_tag="ACZPDN_00495"
     CDS             complement(27113..28045)
                     /locus_tag="ACZPDN_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1297138.1"
                     /translation="MYKVSILVPVYNVEKYLSKCLESILLQTYKNIEIIIINDGTTDS
                     SLEIANLYASKYDFIHVYSYENAGISTTRNRALEKATGDYYLFVDSDDYLDPKMIEKM
                     VDRAIQTNSDIVTCGYCIEYKGLSIPVSPMRKQTMDSLSALRALSQNKGINNYPWGKL
                     YAASCFKNVQFPDEKKGFEDTYTVFKAIYNANRVSIMPNCYYHYVKRPGSLTDHMDIV
                     QAYEMRAAYEYQELCLHQWFPEENFYYDINFYNSDMVILYTLIFFYSRKDQPVYVPAV
                     IDWSKINIFYKIGYRVWRFIACIKFGWSLKWQEN"
     gene            complement(28138..>28226)
                     /gene="rrf"
                     /locus_tag="ACZPDN_00500"
     rRNA            complement(28138..>28226)
                     /gene="rrf"
                     /locus_tag="ACZPDN_00500"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         8532 a         5669 c         3919 g        10106 t
ORIGIN      
        1 tatacatact tgccttggct aaaaagtcaa ataaatctgt atacggcaca tgtttaataa
       61 ttgttggata aattgttttt cctacacctt ttaagacttg taacggcata cgtatcgaat
      121 tgttttcaat cgtataaaaa gcttttgaat gattaatatc gcatccattc acaacaatat
      181 ttcttctttt acattcataa atatattgtg aagtttttat acctgaacca atgacactat
      241 ttaataggct ctgatagaaa tacaaaggat aatttgcctt taaataagcc atttgataag
      301 cgactaaact ataagcataa ctatgtgatt tattaaatcc ataacccgca aattgttcca
      361 ttatagaaaa tatctcattg gaaacagaat ctttgatacg attctttcgt gcacctaaaa
      421 taaattgttc ctttaaattc tgcataacat ccaatttctt tttcgacatt gccttacgaa
      481 gtgtatcggc ttgcgccaaa ctaaatccac caattacttg ggctgtttgc ataacttgtt
      541 cctgatagat catgatccca taagtttctt ttaaaatagg ctctaataaa ggatgtggat
      601 atacaacttt tgaaggatcc ctttttcttt caatatatgt atcaatatgt tgcatcgctc
      661 caggacgata taaagcaagg actacactaa tatcctcaaa tttattgggt tgtatctttt
      721 ggagtaaaga tgtaattcca ctagactcca attgaaatac accaagtgta tttccaaggg
      781 ataatagttg atatgttttc gcatccttta aattgatttt catcatgtct aaagaaatat
      841 gtttctgttc ttttagattt gtgacaattt catgaatcgt tgttagatta cgaattccta
      901 aaaaatccat tttgatcaaa cctaaatctt caagatattc tgccgtaaat tgtgtagctt
      961 gtatatctcc atccacactt acaagaggac aaacattcac aataggctga tctgaaagga
     1021 caattcctgc tgcgtgcaaa gatgtatgtc ttggtaatcc ttcaatactt acacacatct
     1081 gaaataattc aatcaatttt tgatctgatt gaatcctttt tcgaaataaa gcattttcgt
     1141 tataagcctg cattaacgta attttaggcg tattctttaa catttttgaa agctcatcca
     1201 caattcttgc cggaatattc atcacacgtc ctacatcacg aatagcttgt cttgcttgta
     1261 atgtattgaa tgttacaata tgactcacat gatcctgtcc atagagtttg cgaacatatc
     1321 gaatgacttc atctcttctg tcatccggaa aatccgtatc gatatcgggc atagaaatac
     1381 gtgatggatt taaaaatctt tcaaacaaca aattattttg aattggatca atatgcgtaa
     1441 taccaagaca atatgcaact aaagaacctg ctgcactacc acgtcctgga cccacaaata
     1501 tatctttact tctggcatat cggataaagt cccagacaat caaaaaataa tcagtaaatc
     1561 ccatattgat aatgacactc aattcatatt ctagacgatt catgtaaact gcatctagat
     1621 taccgtttaa tcgtttttct aatccctttt tacaaagttt caccaaataa tcactagaat
     1681 caatatgcag cttattttca aacactggaa gctttgattt ttgaaacgcc atttgaacat
     1741 tgcacattct tgcgatttgt tctgtagcca acaaatcacg ttcatcatac aacgcttcca
     1801 tttcttgtgg acttcttaaa tatctttgaa attgaacatc taagccttgg ttttctatac
     1861 ttgtttgttt ggcaatagct cttaatgctt gtaatgattt tacatcttca ctcttctcat
     1921 aaagaatacg cgatagtgct acacacttac aatcaagttg tttcaaaatc ttattgtcga
     1981 ctcttttttt tcctgaatca ttcatagata tggaaacata aaaatccgaa aaagaatttt
     2041 tacatagttc tacaaacgca cttaactttt ctaaatcctg atgttcaata tatgcactaa
     2101 atgtatcttt tccatcactt gaagtaagca caacacaatc tttggcatac tttataactt
     2161 catcaaaaga tacattttct tttatgatcg aacttaattt atataaattc tgtagaccca
     2221 tatcattctt cgctagaaga ataaaatgaa agggctcttc attatagatg gaatcaactt
     2281 ctaaaccaat aatcgcatgg acattatgtt ttttacaaag ttgatagaac tccatcgttc
     2341 catacatcac attatgatcg gttaaacaaa catgattata tccgagttgt ttttgatgaa
     2401 gaatcatact ttctaaacga attggcgatt ccaataaaga gtatgaactt cgcatatgta
     2461 agtgaaccat agaataccac ctttccattg tagatattat agcataagaa aaagagatag
     2521 cttcttaagc tacctctcga tttgataaaa tttcaataat ttgatcgact tgatcacgcg
     2581 ttaagttttt gattccactt gcacattcgt ggccgccacc accaaattcc atagctacat
     2641 cacgaattgg aatatttctt gatcttaaag atgcactata gtgaattcga tcttccatgc
     2701 gtgtaaataa agcccacatt tcaacacaat caataccagc taaacaatac actttatctt
     2761 tggcatctgt aaagtttaaa ccatactctt cataatcttc acattctaga atcgaataaa
     2821 gaaatttatt tttaagtatt gtatgatttc gtacaaccgt ttcatatctg taatcttcaa
     2881 tcgatttaga gaagttaagt tgttctactt tagtaacatc tacaccttgt tcaaataagt
     2941 attttgcggc atcaaatgta gctgggcgaa cattatttgt cgtaaaacga atgttatctg
     3001 ccgtcaatcc taaatataac attaatgcac tttctacagg aatatttact ttattttctt
     3061 gaagatataa agctaataat tcacaagtag cacttgcctt atcgtcaatc cattcttcat
     3121 cacaaatggt ttctacttgt acgtgatgat cgatacgtat cgattttttt gcgaacttgt
     3181 aacgttcatc atcaatacgt gctgcatttg aagtatctaa aacaatagct aaactagaag
     3241 cgactacgtc atcatctact tcatccatcg ttacattgtt tctttgtgat acaggacaat
     3301 tacttcctaa gcaatagatt tttttatctg gataagcact ttctaaatag tacttcaatc
     3361 caaattgaga tccaatcgca tccatgtcgg caaaaacatg tcgaaagatt gtgattgtat
     3421 taaaatcttt tacactttct aaaatagcat taggcattag ccgtcaaatc cttgtaggca
     3481 tcacgtgcga taactaattc ttcatttgta ggtaataacc atacttgcat ttttgattca
     3541 ggagcactta ataatttttc aactccacga actccatcgt tgtcttctgg ttttacaaca
     3601 acaccaaatg cggataatcc atcacaaatt ctttgacgca ttgcacagtc attttcaccg
     3661 ataccacccg caaagacgat cgcatcagca ccacccatta atccatagta tcctgataca
     3721 acttcaacaa tacggttaac gtaaacattt tgagttaaga ttgcacgtgg atttccttct
     3781 ttgacagctg cttcaatgtc acggccatca ctagagattc cacttacacc taacatacca
     3841 gattttttgt tcatgattgt atccatttca tctggtgtat aaccatattt attttctaag
     3901 aatggaacga ttgcagggtc aatatctcca caacgtgttc ccatcatgat tcctccaaga
     3961 ggtgtaaatc ccattgatgt cttataggat ttaccatttt ttacagctgt gatacttgca
     4021 ccatttccca agtgacaagt gatgatgttc atttcttctt gtgtttttcc ttgaagctca
     4081 gctaaacgtt ttgtcaaata ttcatgactc gttccgtgag ccccatagcg acgtacttta
     4141 tcatttgtat aatattcata aggaagtggg aagatatagt tttcttctgg catactttgg
     4201 tggaatgctg tatcaaatac aaatgtatgt ttacattctg gtaaagcatc tctaaatgaa
     4261 cggtatccga ctaatgcagc tgggttgtgt aaaggagcta atgcacataa ttcttctact
     4321 ttagcttcta catctgcatc tacaggaaca gaatgagaga aatattctcc accatgaaca
     4381 acacggtgtc caatcgcttt gatatcttct aagctattta caactttgtg ttcgatcaaa
     4441 gcttccatta acttcttaac agctactgca tgatctttga tatctaagat ttcctcaatt
     4501 ttttctccat tggctttcat tccaaaaata gaatcattca aaccaatacg ttcaacattt
     4561 ccttgtgcta gaacttcttc ttctggcatc ttaaaaactt ggaatttcaa tgatgaactt
     4621 cctgcattaa cactcataac aatcgacatt ttttgatcct cctaaaatat ttcttttaat
     4681 atttcgattt gttcaacatc ctcatgttgt aaaatccatg catctaataa ttttttctta
     4741 gcgacatatc caatcgctgc ttcatatttt tccagtaatt ttgtagctcg atgaaccgaa
     4801 tccatcgccg ctgcacgaga aatctctagt tcctcactta tttcagtata agagaaatct
     4861 tcttcatagt aaagagataa aatctctctt tgacgtttag tcaacaagtc tccgtataca
     4921 tcaaataaat cattcgcatc taattttgaa atcattattc ttctatacct tttgtgatgc
     4981 ccatcaagaa tgcattaata tcaaaaggtc ttaaatccgc tggtttttct cctaaaccta
     5041 agaaacgaac tggtaaatgt aacaagtctc gaatcgcaag cacaacgcca cctttggcag
     5101 ttccatccat cttcgttaaa acaatacctg taacatctgt agcttctaag aatacttttg
     5161 cctgagaaat accattttgt cctgtcgtcg catctaaaac taaccaaact tcttgtggag
     5221 ccccctcaat ttctctttga acaacacgat gcatcttgct taattcattc attaaatttg
     5281 tcttattttg tagacgacct gcagtatcac aaatcaaaat atctacatct ttttctttgg
     5341 catagcgaca agcatctaca agtacagaac ttggatcttg attcattttt ccttttatac
     5401 aaggaacacc taaacgattt gcccaagtat caatttggtc aatggctcct gcacggaatg
     5461 tatcggctgc tgcaacagcc accttctttc cttgctcttt gtaatattga attaatttag
     5521 cgcttgtagt cgttttacca ctaccattta cgccaaccat caaaatgaca gttggacctt
     5581 cttcattcat tttaattggt tcatcgcttg actcatcata aaaatcataa agaatagata
     5641 ttaagtaatc taacatagaa tcataatctt taatatgttt cccattggat tcaaacgcat
     5701 ccacaatttt ttgtgctgta tgaataccca catcactctc aagtagaata accatgattt
     5761 gttctaataa atcatcatca attccatgaa agttattgga taaagcacgc atacgatctc
     5821 caaaactttt ttttgaacga tcaagacccg ataaatactt atctttatct tcacttttca
     5881 cgaaagcttc tttgattttt gaaaagaccg acattactct tcctccttag ctatatgtac
     5941 tgcatctttt agttgcacct ttaatacttt actaacacca tctttttgca tcgtaacacc
     6001 atataaagta tcacactgtt ccatagttcc tggtctatgt gttaccgcaa taaattgaga
     6061 ttgaccacga taatgcgata gataacgtgc aaaacgttct acattagcct ggtctaatgc
     6121 cgcttcaacc tcatcgaaga tacataatgg cattgttctt gcttttaata tcgcaaacaa
     6181 tacactgatc gcaatcaatg ctttttctcc accagagaat gtttgaatat ttttaaccat
     6241 ctttccagga ggctgaacat caatatcaat tcccgtattt aatacatcgt ccggatctac
     6301 catagataag cttgcacgac ctccaccaaa catggcttta aatacgccat ctaattccgc
     6361 gttaatttta ttaaacatat ctgtaaattg actgatcatt gtcttatcca tttcatcaat
     6421 ggctgccaaa atttgttgac ttgccttttc taaatcttct ttttgacttg tcataaaatc
     6481 aaagcgttct ttgacttctt tatattcatt tggagcatcc aagtttacat ttcccaaacg
     6541 agaaatggct tgacgaagtt gaataacttc ttctttagca ctttcaattt ctaaatcttc
     6601 ctttttcgtt aatgcatatt cataagtcat ttcataatct gtacttaagc ggttcaatgc
     6661 attttccaat tgtgttttga ccttcacaat ttccatctca taattatgga tttgcgattg
     6721 tttagaattc atttctcttc gaactaaacg aatttgattt tcaagctgtt ccgcatcatt
     6781 tcccttatca aagcgtgact gacgcaaact ttgtatttct aaagacaatg aatctaatac
     6841 tgcatgcatt ttggacatct gcacaactaa atcatcttgt gctaattctg cattctcttc
     6901 aatatcgaca cctagttctt ggtattctgc aagaattgaa tcatattttg ccttttttgt
     6961 tgcgtaaata ttctctaatt tagccaattc aatctgtaaa gttacaatcg catccttttc
     7021 tttttgaagt ttttgcgtaa gaatttccgt ttcattcaaa caactatcgg ctttgatttt
     7081 ttgtccctca attttagaat taattgtatc taattcttga cgcattgtga caggggtgct
     7141 ttggtttttc gtaacaccac ctgtcatact accaccagta tgaacgatgt ctccatctaa
     7201 agtaacaatc ttatatgcat agcgtagtcg ttttgcggtt tcattggcat tttgtagtgt
     7261 atccacaaca atgacattgc ctaacaatct ttctttaaca ggatcaaaga tttctttaca
     7321 atccacacat tcgctcgcaa agcctaaaaa gcctgggcta gttgaggcaa tcgtaatgac
     7381 ttgttcattc atttttctag gatgacatac acttaatggt aagaaagtag cacgtccgct
     7441 acggtttctt tttaagaaag aaatggcatt tcttgcatcc gcctcatttt tagtgatgat
     7501 ttgatagata cttccaccta aagcagcatt gaccgccaat gctttatctg catgagcaat
     7561 caataattca ctaacaactc cataaacgcc ggataaagaa tttttagcct gcataacaga
     7621 gcgtacacct tgttggtgtg caaaaggttg cttcatcata ttttctaata cttgtcgacg
     7681 attttgaagt tgtgtcaaaa tattgtttgc ctgatctgat tcataacgtg ctttagatat
     7741 cttcattttt agatcattca caatactacg acgattgttt aaatcttgtt gcgtctgcat
     7801 taaacgctga tgacgatctt gatattcaaa acgagcttcc tctaacattg cctgaatttc
     7861 tttttgacga gcctttttat cagctacctt caacatatat tttcgctttt catcttgttc
     7921 gatcttacgt ctttcaagct gatagttttc ttccatcgcc tttgtatatt ttccttgcaa
     7981 ctcattgatt tgtttatcaa gagcatacat ttcttttcga atactttcta aacgagtttc
     8041 ttgctttaat aattctgcat tttcagatgt atgcatagct tgaatatcaa acaattcttt
     8101 attaatttga ttgattttct cattgtattg atcaatatct tctacaagta cactaatttc
     8161 aatttttgat aatttttctc ttaaagaaat atatttttct gctttttttg cttgtttttc
     8221 caaaggacca atttgtcttt caagctcatc tagaatgtcc tgcaaacgat ctagattttc
     8281 ttttgtctgc tctaattttg acaatgacac cttttttctt tttttatatt ttgcgacacc
     8341 cgcagcttct tcaaacaaag aacgcctgtc ttctggttta gcatccgcaa aagaagagat
     8401 gtttccctgt gtaataatac ttaaggaatc tttacctaaa ccagtatcca taaccaaatc
     8461 attaatatct tttagtcgac atggggtttt atttataaaa taacttgctt cattatttgc
     8521 gcgaagtatt tgtcgtgtaa tttcgatttc atcaaaatcc gaatcaaaaa cacgtgttga
     8581 attgtcaaac accaatgtaa cgcgtgccat atttacaggt tttctatact cacttcccga
     8641 gaagataata tctgacatat tcgttccaga acgaagtgat tttacactct gttcacctaa
     8701 cacccaacga atagcgtcat tcacattact tttaccgcat ccgtttggtc caactatacc
     8761 tgtaatatct tgatcaaatt gaatcactgt tttgtccgca aagcttttaa agccttgtaa
     8821 ttcaatacgt tttaaaaaca tcaagaccct ccactctaac tgaattatta tatcaaaaaa
     8881 cacacgcaaa atatatctag tatctatttc tagattgtgt attatttgtt attatgttat
     8941 agtatgtgta tgaacaagaa agatagtatg cggtatgttt atcctctttg ttttggtgaa
     9001 gaagtaggaa actgtattac acatggagtt atggcaattt tgattctatt ctcacttcct
     9061 tattatagtg ttcgcgccta tttaaggggt ggtactatag aaaccgttgg aacagctgtg
     9121 tttttgattt gcctactctt catgtttgcc ggatcttgtc tttaccatag catgccacat
     9181 gaaacaacac ataagtatgt atttagaaag ctagatcata ttatgatctt actagctatt
     9241 gcaggtacct acacgcctat ttgtttaaag cttatgaata attggaaagg atatactata
     9301 ttagctattg aatgggttat ggtgattatg ggtatcttat taaaatcgat tgcgacaaaa
     9361 acttatccga agttatcaat gacaatttat atggtaatgg gatggttagc catctttatt
     9421 ttgccaacat tgattaaaaa aacacagccc atctttatta ttttgattgc ccttggtggt
     9481 atcatgtata caattggaac tttcttttat tcaaaaccac aaaaaagata cttccatttt
     9541 gtatggcaca ttttcattat cctagcaagt atttgccact tgttagccat tctgtatttt
     9601 atctaaaaag aaaaacctat attgtaaaat ataggtcctt tttttaaaac attcgagcaa
     9661 tatcattata tgtagcataa ataaagagta tcatcaataa tacaaaactt gccaaaataa
     9721 tattttcaac aattttagtt gaaacttttc gtccaatcaa tttctcaatt agtaaaatca
     9781 aaattcttcc gccatctaat gcaggaattg gaagtgcatt gaagatacca atatttaatg
     9841 agatcatcgc aaataaacta aaatacatat ctaatccata ttgtacagat ttacttgtta
     9901 catttaatat gccaactgga cccgataaat tttcaagtcc ttgtccacga ataatcatat
     9961 tcaaagattt aaatatagtt gttgcactat cccaagtatt ttgacaaccc acccataatg
    10021 attgatacca tgggattttt ttggcatacg caatcgcctt gtagccaagt gtatagcctt
    10081 ccatatcttt atcataagat ggagttaatg tcgttttaaa tgttgtttta tctcttttca
    10141 cagttaatgt cagtgtatca tgatgatatt gaataaactc taaaatttca tattgtgttt
    10201 taggttgaat actatttcca tcttcactta aaactttgat gatatgatca tccttttcaa
    10261 gtcctgcctt ttgagcaact gagttttctt caactacata gacaacaggt ttagcttctt
    10321 caaccacata gccttgtact aaagcaactc ccatataaat aatccatgcc aaaagaatat
    10381 tcatcacaat tcctgcaatc ataatacaga cttgtttcca tttttctata ccatttaatc
    10441 gtctgttttc tggtatatct tttaaccaac tatcttcttc actttgggat ccatcttctt
    10501 ctccagccat cataacatag ccaccaaaag gaatggcacg aatcgaaaaa gcagtttctt
    10561 ttccttgttt ttgataaagg catggaccca taccaatcga aaattcttta caataaacac
    10621 caaaatgttt tgcaactaaa aaatgtccta attcatgtaa aatgacaatc acacttaaca
    10681 tgataataaa tgcgatcaat ccaataacaa aatccatcta tatcctcctt agagaatgat
    10741 tgtatataac acactaaata acataatatt caataatagt gagtctacac gatccaaaac
    10801 cccaccatgt cctggtaata ggtttgaaaa atcttttacg gcaaaatgac gttttatagc
    10861 actaaaacac aaatctccaa gttctgcagt aattggacat aataaacata agagtgtatt
    10921 taatacagga tttaaatttg aaacataact aaaacttaca actaagctca atataaatcc
    10981 aaatacaatg cctcctacaa aaccttccca actcttttta ggtgagattc ttggattcat
    11041 tttatgtttc ccaatagcgc gccctacaaa ataagctcct gtatcacttc cgtatgtagc
    11101 aaatacaatg gtccataaat attgatgtac acctgagatt tgtccaaccg catgatatac
    11161 tagtgagaaa attgttacat atgaaatgca cacaaaggaa tccataattg agaaattctc
    11221 aacaaataca actaaactcc acaaaaatag cacaatcagt gttatgacta ctaaaaagta
    11281 tttatcgggt aagattcttg tgaatatgac tgccaaagca cttaatggca tgacatattt
    11341 aggccatttt ttaaaatcag gttgaatgtg catccattca taagcgcctg cacatacaat
    11401 aaataaagcc aaagcttcta gccaaagccc accaagtgct acaggtaaag caacaacaag
    11461 aatgatacct attgcggtaa taattctttg tttcatgatg ttaagcctcc aaatctgcga
    11521 tctcttgagg caaattcatc taagcaatct tttaatgctt tttcatcaaa atccggccat
    11581 gcgactggcg taaaaatcaa ttcagcatag gcgagttgcc ataataaata attggaaata
    11641 cgtagttctc cacttgtacg aatcatcaaa tcgatatctt ctggaacaaa taaatactta
    11701 gagaattctt cttcggttaa atcgttttct cttttacctg aagctacttc gtctgcatat
    11761 ttttttattg acaaaagcat ttctcggcga gaaccataat tgatagctaa acataattct
    11821 aagccagtat tctttgaagt catatttaca gctttattga tgacatcttg tgttgctttt
    11881 ggaaattttt ctaattctcc cgcaaatgta acttgaatat tcttttccat taattcattg
    11941 ataaatttat taaagaataa gccaggtaac ttgcataaat aagaaacttc atcttcgggt
    12001 cttttccagt tttctgttga aaaagcatat aaaattaatt tttgaattcc catatcctga
    12061 gcagctaaag ccacagtacg aattgtatct gcaccctttt tgtgtcctgc tgttcttggt
    12121 aagcctcttt ttttggccca acgaccatta ccatccataa ttatagctac tgtttttggt
    12181 gccataaaaa cctccttttt aaaaaagtcc aaatcaagtt tggacttaga catttaatat
    12241 ttcttttttc tttgtttcag taattgaatc aacatcttta atatatttat ctgttaattt
    12301 ttgtgattct tccaatgttt gtttcttttc atcttcagga agttctttat ctttcttgat
    12361 tgcatcattt gcatcacgac gtacgttacg aatagcaact ttagcttctt caccatattt
    12421 ttgagcatct tttgctaatt ctttacgacg atcttctgtc aattgaggaa cttgaatacg
    12481 aataacatct gcttcagcct gtggatttaa tcctaagtta gtagcttgaa tggcatgtga
    12541 aataccttta acgattgaac gatcgtaagg tttaacaacc aattgtgttc cttctacaac
    12601 tgagatttgt gcaatctgat taattggagt catagctcca taatattcaa cttgtacacg
    12661 ttctagcatt tgtccactag cacgtcctgt acggatttga cgtaggtttg attctaagtt
    12721 atcaattgcc cccatcattt tttcttctgc atttaataaa atatcactca tgttatttct
    12781 ccttatttct ttttagtgat tgtagtacca tttacttcac ggcgaacagc cttaacaata
    12841 tttcctggtt cattcatatt gaatacaact aaatcaatat cattatccat acacatacta
    12901 atagctgttg aatccatgac ttgtaaacct tcatttagta cgtccatata atttagtgta
    12961 tcatattttt tcgcgttcgg attcttttta ggatccgaat catatacacc atcaacacca
    13021 ttttttgcca ttaagataac atctgcatta atttcagaag cacgtaatgc agcggttgta
    13081 tctgttgaga agtatggaga acctgttcca gctccaaaaa taacgattct tcctttttct
    13141 aagtgacgca atgcacgtct taaaatgaat ggttcagcaa ctttttgcat ttcaacagct
    13201 gtttgaacac gtgtttgcac accttcttgt tctaaagcat tttgaactgc taatgcattg
    13261 attactgtac ctaacatacc catataatca gcttgagcgc gattcattcc catttcagcc
    13321 atcattttac cacgaatgaa gttgccacca ccgacaacga ttgcaagttc tactcccaat
    13381 ttttgaactt cttttaattc ttttgctaat ctctttaaga tttctggatc aaaagcagct
    13441 tcaggactag ataatgcttc accacttaat tttaataaaa cacgtttata cataatattg
    13501 ctccttattg aattatcata acaattatac actatctttc attaaagaaa aagaaaaacc
    13561 cttggaattt ccaagggtta gaagtaaaag ttaaattact tagccatttg agcagcaact
    13621 tcttcagcaa agttttcttc gcgtttttcg ataccttcac cagtttggaa acgtacgaaa
    13681 gttactactt cagctttgtt gtctttcaag aattgagaaa ccttagtttt tggttctaag
    13741 aaatattctt gatctaataa gcattggtct ttaaagtgtt tatcaacttt tccttttaag
    13801 attcctacca atactttttc tggcttttta gccatgttag gatcagcctt catcatttgt
    13861 aattgaagtt cacgttcgtg ttcaacttct tcacttggta cttcagcttg agatacatat
    13921 tttggagcca tactagcaac ttgcattgct aagtttttag ctacagtagc atcttcagtt
    13981 cctttaacaa ctacaacagc agaaatggat ccacccatgt gcatgtatga accgaaaatt
    14041 tcatcatctg ctttttcaac aacagcaaca cgacggaaag aaattttttc tccgatagta
    14101 gctgtagcgt tgataatcaa atcattgata gttccttcag cagtttggat gtttaaagct
    14161 tcttcagttg aagcaggttt gtttgcaaga attgcttctt gtaaagtgtt catcaaacct
    14221 aagaattgtt cattcttact tacaaagtct gtttctgagt taacttcgaa taatacagca
    14281 gtgtttccac taacagcaac acgagttaaa ccttcagcag caatacgtcc ttctttttta
    14341 gcagattttg cgattccttt ttcacgtaac cagtcaacag cttttgcaat gtcaccttca
    14401 cattcagtta aggctttttt gcaatccatc ataccagcac ctgttttttc acgtaattct
    14461 tttacttgag cagcagtgat agccatttta gcatcctcct atatttttaa ttattcagca
    14521 gcttttgctt cttcagcctt agcagctggt tttacgtcat cacctttttt gttgaaagga
    14581 cgacggtttt tacgttcgaa gttacgtttt ctacgttctt catttctttg acgacgacgg
    14641 cgttcgtttt cagcgatttg tgcttcaaca tttttgatta cgtcttccat agttacatca
    14701 tcttcagttt catctaagta agccatttct aatagaccac ccttagcatc aacgatagca
    14761 tcagctaaga ttgttgtaac taatttaatt gagcgaactg catcatcatt agaagcgatt
    14821 ggataatcag catcttctgg atcacagttt gtatctaaca tagcgaatac agggattcct
    14881 aattttttag cttcagcgat agcattgtgt tcttcttttg gatcgataac aactaatgca
    14941 tctggaagtt tcttcatttc tttgattcca cctaaagaac cttccaattt agctttttct
    15001 ttacgtaagt tagcttgttc tttcttagta taaacttcga tttttccgct agcttccatt
    15061 tcttcgattt caactaaacg tttgatacgt ttttggattg tacggaagtt tgttaataaa
    15121 ccacctaacc aacgtttgtt aacatagaaa gatcctgaac gtaaagcttg ttcttcaaca
    15181 acttcagcag cttgtttctt tgttcctacg aacaatactt ttccaccacg ttcgctaata
    15241 gcttttaatt cattgtaagc agcttcgatt tgtgcttgag tttggtttaa atctacgata
    15301 taaaccccgt tgcgacttgt gtagatgaat ggtttcatct ttgggttcca acggcgagtc
    15361 tggtgaccaa agtgaacacc gttctctaac attttcttca ttgatacgat tgacataata
    15421 gtcctacctt tccgtttctc ctccatcatt tcagacgcat gcaacacctt cgtgcacggg
    15481 cacacgaatc catgatgtgt gtaataagcg caatcacgcg cttttacatt atagcaatat
    15541 cctttttaac ttgcaagatg ttatttctca aaaggatgcg cttttttata agccttcact
    15601 aactgtgcag tcgtgacatg cgtataaatt tgcgttgttg ataaagatga atggcctagt
    15661 aattcttgaa caacacgtat gtccgctcca ttatccaata aatgtgtcgc aaaactatgt
    15721 ctaagcatat gaggatgtaa tttcatatgc atgccaattt tttttgcgtg tttatccatt
    15781 agatattgaa tgcctcttga agtaagtgat tttcctttta gattcacaaa tacatgatcg
    15841 tctattgcgt attttgtcca aaacttactt ttatactcca ataattcttt ttcaaaacct
    15901 tggtaaaaag gcacaatacg atctttatcc cctttaccac ggatatgcac aatgcgatta
    15961 ttcaaatcaa tttgtttcca ttctagattc acacattcac tgacacgtaa tccacatgca
    16021 tacatgattg taaataatat acgatcgcga tattcatcat ccttttcatc attgtaagaa
    16081 ttcaaaaatt cctgaatctc ttctacaaac aaaaaatctg gtatttgttt ggattttttt
    16141 ggtgtttgaa tccctatcaa tggattttca ataatcccca tatattcagc caaatatgta
    16201 taaaacgaac gatacacact cattttacga gctattgagc ttgcttttag atgatctaac
    16261 gtagctagaa aattcataaa agtcaatcta tcgcaagatt caaatgaatc aatagattga
    16321 cttgttaaat attctttaaa ttggtttaaa tcatttttgt atgattcaat cgttttttca
    16381 gattgggtat ttttacgata aatatatttt aaaaaccgct caatcaattc atccatcgac
    16441 ttcatccttg atttgtgcaa tacgagctaa tgcttgagaa gcaaattttt ctttacgctc
    16501 atgcttcttg catttttcat ttaagtgcat aataccaaag tttgcattca tcggctgaaa
    16561 atgttttgca tcacaatgcg taatgtaata ggcttgtgag cccataacgc atgtatttcc
    16621 aaaacaaata ggctctttac cttgtatata taatgccata ttaattccgg caatcaatcc
    16681 agatgcgcaa gattcaacat acccttcaac ccctgtcaat tgtcctgcaa agaaaagatc
    16741 atcgcgtttt ttagattgat atgtttcttt tagaacaact ggacttgata agtatgaatt
    16801 tcgatgcata acaccataac gagtaatctt taaattttct aatcctggaa tcaaggcaaa
    16861 cactcttctt tgttcaggcc aagttaaatg tgtttgaaaa ccaacaatat tatacatact
    16921 ggcagccaca ttatcttggc gcaattgaac aacagctaca ggatgagaat ccttatcttt
    16981 ttcaagtccg actggtttta acggaccaaa caacatcgtc ttttttccac gtcgagccat
    17041 ttcttcaatt ggcatacatc cttcaaaata agtttctttt tcaaaatcat gcaaattcac
    17101 acattctgca tgaatcaagg catcataaaa tacttcaaat tcatctgcat tcattggaca
    17161 attaatgtag cttgcttctc ctttgtcata acgagattta taataggctt tactaaaatc
    17221 aatagaatcc ttttcaataa ttggtgctgc agcatcataa aaatgaaatg tatcttctgc
    17281 cgtaaaatca tgaatagcct tagctaaagt gtcacttgtc aaaggtccag tagcgataat
    17341 acatggtcct tcaggtaaac ttgtcatttc ttcattcacc acttcgacca acggatgatt
    17401 tttaattata tctgttatat attgagaaaa cgtttctcga tcaaccgcta atgcacttcc
    17461 tgcaggtacc gcatgcatat cggcagattt cataattaaa gaatccatct gacgaagttc
    17521 ttcttttaat atacctactg cattattcat cgcatttgaa cgaagtgaat ttgaacatac
    17581 aagttctgca aaacgatccg tatgaaaggc tggactttcc tttttaggac gcatttcaac
    17641 taatcgaacg ggaatgtttc tttttataag ctgccaagtt gcctcacatc cggcaagtcc
    17701 tgctcctata actgtaactc tttccattat ttttcttcct cttttttctt aggttccttt
    17761 tttatataat ggcattttgg ataatttgaa caaccaataa aagttgtgcc aaaacgcgat
    17821 tttcttctta caagctcatg cccacattct ggacacatct cacctgttgg ttcaggttta
    17881 gctttctttt ttaaagattc aattgtctta cattccggat aattcgaaca tgcccagaat
    17941 tgaccatagc gacctctttt aataaccatt ttagctccgc agtttggaca aatcttttct
    18001 tcttttgcct cttctggaag ttcttcctca ttattttctg tgtagcgaca tgttgggaaa
    18061 ttgatacatg aaatgaactt accaaaacgt ccattacgat acacaagttc gccaccacat
    18121 tcaggacatg tacgtcctac acgttccaat tcttttttcg gcattttttc ataggcatct
    18181 tctacgagtg gcgcaaactt atcgtagaat ttatgcatat aagatatata atcttcgtta
    18241 ccttcggcaa tttcatctaa tgttttttcc atatccgctg tatattcaac gttgattaca
    18301 cttgaaaaat attgttgaag cttttcattt gtcaaaatac cttgttcact agggaaaaat
    18361 acttttgtct ttgaagtttc actcgcctta tccaaagatg catagccacg tttttgtaaa
    18421 gtatcaatga tcgtcgcata tgtacttggt ctaccaatgg atttttcttc tagatcctta
    18481 atcaattttg cttctgtata tcgtgctggt ggttctgtaa agtgctgttt cgaaattggt
    18541 gttacatttt ttaagacttc attttcctct aatgtcggaa gcaatgcatc actttgtttt
    18601 tcatattttg aatagacttt ataataacca tcaaaagtca ttgtttgtcc acttgcacta
    18661 aattccaaat cattgtttgt gattttgata ctcgttacat cataaactgc atctttcatt
    18721 aaagatgcca gagtacgagc ataaatcaat gaatacaaac gatattgatc attcgttaaa
    18781 tggtctttga ttgattctgg cgtacgattg atattcgtag gacgaatggc ttcgtgtgca
    18841 tcttgcgtat tcttaccatt tttttgatga acatgaccaa catattcttt accatatgta
    18901 tttgtgataa attctttggc gtctgaaaca aaaatatcgg ataaacgcgt tgaatccgaa
    18961 cgcatatatg taatcaaacc ttccatattt ccgcctagat caataccttc atacattttt
    19021 tgagcaacgc tcattgtttt ctttgcccca aaacctaatt ttgtactagc ttcctgttgc
    19081 atagtagatg ttgtaaatgg aagtttgggt tgttttttct tttgtttttt agaaatcgat
    19141 gaaacaacat actccccatt acatctttct aataacgcct tagcctcttc ttcatttttg
    19201 atttttggct ttttaccttc tactttaact aaatcagctt caaaagcttt attctgcttt
    19261 ttacattgtg catgaattgt ccaatattct tcttgtttaa atgctttgat ttcattttca
    19321 cgatccacaa tcaacttcaa tgccacggat tgcacacgac ctgctgattt agactgaatc
    19381 ttattctgta atagtttact taatttaaat ccaataattc gatctaacat acgacgtgtt
    19441 tcttgtgatt tcactagatc catatcaatt tgtcttggtt ctttcatcgc ttcaagaatc
    19501 gctggttttg tgatttcatg gaatacaata cgattgttgt cctcaatatt taaatccaaa
    19561 acacgagcta aatgccaggc aattgcttct ccttcacggt ccgggtcact cgcaagatag
    19621 atatcttttg ccttagaagc atattctttt aattctttta cgacttcttt tttttcttta
    19681 ctaattttat atttaggttc aaaatcatgt tctacatcga ttcctagacc ttcttttcca
    19741 cttgtcgcca aatcacacac atggcctttg ctggaaacga ctctgtaatc ttttcctaaa
    19801 tatttctcaa tcgtcttcga ttttgaaggc gattccacta taactaaatg ttttttcatt
    19861 ttattacccc atattcacgc tatataaaac attaaaaatt tcattttgtc aatcctagat
    19921 atcatcaaca tctttaatat ccaccaaaat attggcacca ttactaataa gataattaca
    19981 cccttgatgt ttttcatctt gaaaagcagt tggtacacaa tagatgggta cacttaattc
    20041 taagcattca tttactgtga tcatcgtacc acttttatgg gctgcttcta tcacaactaa
    20101 actgtcactt aatgcggcaa ttaaacgatt tcgccatgga aagtgatacg ccaaaggttt
    20161 gacgtgcatt ggatattcac taatgatcaa tccattattt ttcatccttt caaataaata
    20221 tgtgttttcc tttggataaa tcacattaat accacagcca agtacaccaa ttgtttttgt
    20281 cgcattataa tgtgctctag catcaattcc tttcgcaagt ccagatacaa tgacatactt
    20341 ggattgtagt ctttgtacaa tactatccgt attctgtaaa gcttgcgttg aacagtttct
    20401 tgctccaaca atacccacgc ctttttcttt caataaattg atatctcctt gatagaaaat
    20461 gatccatggt ggatagcgaa gtcttttaaa tgcatctgga tatgcatcat ccataattgt
    20521 aagatacgca tatggataat caatgatgga atagttttca tgtgccttta tggcattgcc
    20581 aatctttttc caatctccat catattggat ggcataccat aaaatagctt gcctgtttat
    20641 tttcatatac tcttatacga aaaaaaagag actttttcta ataaaagtct aaaaaagtct
    20701 aatttcttca agttttttta caggcccata gcttctacga tagaaatctt gaagtccata
    20761 ttgatttaaa gcttcaatat gtgcttttgt aggataccct ttatgtttag ctaaaccata
    20821 ctgtggatac atttcatcta gttttttcat ccaacgatct ctggttactt ttgctaaaat
    20881 acttgcggcc gcaatggata atgatttttg atctcccttt acgatagctt gaaaaggttt
    20941 atcaatatca ggtaatggca tcgcatcact taacacaaaa tcacattgtg ccttatttgc
    21001 gatttccatc atcgcttctt gtgtggcacg atagatattc ttcttatcga tcgtcttttc
    21061 atctaccaca agaatttgat aatacaaagc atcttgaata attgttttaa acaacgcttc
    21121 acgctttttt tcactcaatt tctttgaatc gtcaatcaat gcattttgat atcccttagg
    21181 aaaaattacg cctgcaacca tcaatggacc ggcaataggt cctcgtcctg cttcatccat
    21241 tcctagaaca ctttgatttt gatcccaata atcatgttct aaaggtctat caagcatgtg
    21301 gtttctccca agtaatcaag ccacatttat tttctcgaat atctttgaca aaacttaaca
    21361 tcaaacgatt ctcatcgatt tcacccttta ataaataacc acgttttatg gcaatattct
    21421 gtaatgtttc ataaggtgta ttcgcaattt ctacatgata tacactatca aagatttctg
    21481 gtttttcgct cattaaatat tcacaggcaa ataaagctaa atcatccata tttacaactt
    21541 gatcttttat agaacctaat aaggctaatt tcaagcctac ttgttgatcc tcaaatttag
    21601 gccacaaaac tcctggtgta tctagtaatt ctagagtttg tcctactttg atccattgaa
    21661 gactttttgt cacgcctgga cgatctcctg tttgagctgc ctttcttttc gcaaacgtat
    21721 tgataaatgt gcttttgcca acatttggta ttccacatac catggctcgt aatgctctag
    21781 gtttcattcc tcttcgcttt tgtttttcaa ttaaatttat acataattca ttggatgcct
    21841 taatcaattg ttttgaaaaa ttttcatgaa taaaatctaa tgccaatact ttttgatttt
    21901 ctgtttctaa cgcacttacc cattgtttcg taagttcttt atctgcctta tctttcttcg
    21961 ataaaagaat caatcttggc ttattttgaa tcaattgatc caacattgga ttcttgctgg
    22021 catctggcat tcttgcatca cgaatctcaa tcacaaaatc tacctttttt aaattttctt
    22081 ccatttgcct tttggctttg gtcatatgac ctggaaacca ttgtacattt gccactaatc
    22141 tttcactcct atatctgaaa ttggtaataa gactaacatt ttctttgcga ataattgtga
    22201 tttcttaaca ggacctacat atctagaatc tttagaatag ggtctgttat ctcccatcac
    22261 atagtattca tcatccccta gtttaacttc ttcaaaatct tgaacatccg tattatctga
    22321 atttggcact ttgttgaagt agccaaattt atctacacat gactgacgat aatctggatc
    22381 tatatattgt gtctcatcca atacttttcc attaataaat acgatatcat ttacgcaact
    22441 tatagtatct cctggcatac caataacacg ttttacccaa tgtgtttttt gtcctttttc
    22501 ttccatagtt acgacaacga tatctcctcg tttgacacca tttaagagta cacctccaac
    22561 attggtaaag ccaaattctc catcttttaa tgttggatac atacttcttc catcaacacg
    22621 tactgggtgt gctacaaagt tcacaaataa taaaatgacg atagcactta ctacaaaaac
    22681 ttttataaac cctagtatat cttcgagtaa tgtacgttca tcatcctcgt tatatcttaa
    22741 ctcacttttt ttagctttct tcataatttt ctatccccct aattgttata aatctattat
    22801 aaatcttttc agaaaaaaaa gcctgaaaat cagacttttt taaacaatta acgaacttct
    22861 ttaagacgag cagctttacc tgaacggtta cgtaagtaag ataatttgtt acgacgtact
    22921 ttaccgtgac gaactacttc aatgtggtca atgattggtg agtgtaaagg gaatttacgt
    22981 tcaacaccaa tttggttaga aatcttacga acagtaaatg tttcagagat tcctccacct
    23041 gcacgttcaa ttacaacacc ttcgaatacc tggatacggc tcttgtcacc ttctttgatt
    23101 ttaacgtgaa cacgaacagt actaccagaa cggaatgctg gaatgtcagt ttttaattgt
    23161 gattttgtta cttcattaac taatgctaag ttcattttct tttctccttt tcttctaata
    23221 tttggttatc agttcattca cacaatgtgc cagcggaact acctatgcgt aaacgcctat
    23281 ttatattatc atgtcaaccc attttacgca agattaattt ttgtttttta acacggtatc
    23341 aactactttt tgatcttctt tatctaattc taaattttct aatagatccg gacgatgtaa
    23401 ggcagttcgt ttcaaagatt cttcatggcg atatttttta atattcgcat gatgaccact
    23461 caataataca tcaggaactc tttttccatt atatacttca ggacgagtat attgaggata
    23521 ttctaaaagc ccatttgaaa atgaatcatc atcacttgaa tcctgtctaa taacaccagg
    23581 taagatacgt aatatgcaat cacacataac catacatgca ctttctccac cggttaagac
    23641 aaagtcacct aaagacattt gaatatccac ataatcacga attctttcat caaagccttc
    23701 ataatgtcca catataaaaa ttaaatgttc ctttaaggat aattcttttg cgatttgttg
    23761 attaaacgtt ttgccttgag gtgttaataa gatgactgta ctattttctg tacgaatcga
    23821 ttctaatgca tctaatattg gttggcacat caaaaccatt ccggctccac caccataagg
    23881 cgtgtcatct acatgaccat gtttttcttt tgtaaacttt cgaaaatcaa tcgtttcaaa
    23941 ttcaaacaaa tctttttctt tggcacgctt aagaatactt gtcgtcatta atggttcaaa
    24001 atattctggg aataatgtta atactgatat tttcatcgta aaccatccat ttcacgaatt
    24061 ataatttcat gcgtatcttt atttacatct acaataaatg ctggtacaaa aggtaataaa
    24121 aaacttgtat tttttgattt tacacgaagt actaaattgg cacctgtttc taatatatct
    24181 acaacttcac caagattttc tttttcctca ttatatacag tacaattcat caattcatga
    24241 taataatatt gaccttcttc aagttctggt aaatcatctt gagcgatgaa tagattcttt
    24301 gtctttaatt gttctgcttt ttcaatggaa tcaattcctt caaaataaca atatccaaac
    24361 cctttttgtt cgcgatagcg cactacagtt aaagtacggc catcttctaa atgtaagact
    24421 cttccttttt caaaacgatg ttggggatca tctgtcacaa gatataattt acattcaccc
    24481 ttaagtccat gcgtattgat gatttgagct acttttatca tatatcctcc taaaaaaatc
    24541 aggatgtgaa atcacatcct aatacgcttc aaattttact gaaattcttt tgtgtacatc
    24601 acgtgccgca atagacatca tctgacgaat actgctagcc ataattcctt tgcgaccaat
    24661 taaacgggca acatcttcac tattggcata tacatacaat aaaatttcat tttcatttgt
    24721 agttgccatt gtttttacag ataatgcttt ttgatcatct acaagtggta aacaaatgtc
    24781 taataatgtt ttttctaaat caatcattat tttgaacttt tagcttcgtg catttctttt
    24841 aaaacacctt cgtgtgataa aattgaacga actgtatcag ttggttgtgc accattgctt
    24901 aaccatttct ttgttaattc tttgtctaaa gtaactttgt tttcagcttt acttgggttg
    24961 tatgtaccaa tttgttcgat gaaacgacca tcacgaggac tacgtgaatc agctgctact
    25021 atacggtaga aaggagcacc ctttttaccc attcttttta aacgtaattt aaccatattt
    25081 aaattcctac cttttctaat ttattaaagc tcataaagaa taacatgaat aatataaggt
    25141 gtcaagtatt tttactttac accttatatt ttttattttt tcttcttttt ctttttagcc
    25201 ttctgtttct tactaccagt atgctttgac atacctggca ttccaccgcc acgttgcata
    25261 ttctgcaatc cagccatatc tggaagtttt accttaccac tcatcatctt agtcatcatc
    25321 tgcatttgtt ctaattgatt aatcaaacga ttgacatcca tgactttcac acctgcaccc
    25381 ttggcaatac gattttttct tgatgctttt aaacaggatg gattttcacg ttcatactcc
    25441 gtcatagata aaataatggc ttcactcttt ttcatttgtg aattcatttt ttcatcatct
    25501 aaatttttag ctaccttatt aatacctgga atcatattca tcattttctg catagatccc
    25561 attttagaaa cttggcctaa ctgtgctaat aaatcattga atgtaaaaac accttgtttc
    25621 attcgttcag ctgttttttt ctgaacttca atatccaatt tatcttgagc ttgttcaact
    25681 aaagtgacaa tgtctcccat tcccaaaata cgatcagcca tacgatctgg gtggaactct
    25741 tctaaatcat ccactttttc accattggat acaaacaata ctggtacatt tgtgatagat
    25801 cgaactgaca acaatccacc accacgtgca tcgccatcca tttttgtaac aatcaaacca
    25861 gttactgcta attgttcatt aaattctttg gcaacggtaa caatatcttg acctgtcatc
    25921 gcatccacag ttaataagat ttcatctgga acaacaagtt ctttcatttg ttgaagctct
    25981 tgcatcaatg cttcatcaat atgtaaacga ccagccgtat caaagattac taaatctttt
    26041 tgacgatctt tcgcatatgc caatgcctgt ttagccgttt caactgcaga tgtttctggt
    26101 ccacatgtaa atacttctac atcaatactt tttccaagag tttgtaactg ttcgattgcg
    26161 gctggacgaa caacatcaca cgcaaccaat aaaacacgac gagctaattt atttttacag
    26221 taattggcaa tttttgcact tgcagttgtt ttaccagtac cctgtaaacc aaccatcata
    26281 accgttgtca taccttgctt cttaaagtga atacaagcat cttcttcacc taataatact
    26341 tgtaattcat catgaacaat tttaacaacc atttgacttg gatttaatga atccaagacc
    26401 tcttgtccaa gtgctttttc ttttacattt tcgacaaagc tcttaactac atcataatta
    26461 acgtcagctt ccaataatga catgcgcact tcttttaaca tgtctgtcat atttttttca
    26521 gtcagtttcc cttgtcctga aatattttta aatgctttat ttaagcgttc acttaatgat
    26581 tcaaatgcca taaataatta tctccctaaa ctctttaagt ttacctaatt ttcatttaaa
    26641 ttacaatgga atgccagacg actttgtcca ttttttaaat aaatagtacc aacatgctta
    26701 aaaccatgtt taatcataaa attttgcatc ggtttattat ccgtatgcgt atccatacga
    26761 atattatcaa tttttgaaaa acaatattct aaaatgatat tggccatatg tttttttaat
    26821 ccacgagtcg caattcgatg aatcacacca tatggctcat catttaacca ttgaccatca
    26881 tagattttgt tgtaacaagg atcaattcca cagtaaaata caaaactagc ctgaatacca
    26941 tcctcatctt ctaaaacata catttcacct tttgcgatat cttcagacaa tatttctttt
    27001 gatggatatc catttgtcca ttgatttaca tttccacaag aagccataaa ttttctagct
    27061 ctatcgtata tttctaataa tataggcata tcttctaatg ttgccctacg aattaattct
    27121 cttgccattt taaagaccac ccaaacttaa tacacgcgat gaatcgccat acacgatatc
    27181 ctattttata aaatatatta atttttgacc aatcaattac agctggtaca taaacaggtt
    27241 gatcttttct cgaatagaaa aaaatcaaag tgtataaaat taccatatca ctattataaa
    27301 agttaatatc gtaataaaag ttttcttcag gaaaccattg atgcaaacaa agctcttgat
    27361 attcataggc tgcacgcatt tcatatgcct gcacaatatc catatgatca gttaaagatc
    27421 ctggacgttt cacataatga tagtaacaat tcggcattat agatacgcga tttgcattgt
    27481 agattgcctt aaaaactgta tatgtatctt caaaaccttt tttttcatct ggaaattgaa
    27541 catttttaaa gcaagaagct gcatataatt ttccccaagg gtaattatta atccccttat
    27601 tttgagacaa tgctcttagt gcagataaag aatccatagt ttgttttcgc ataggactta
    27661 ctggtatgga taaaccttta tattcaatac aatatccgca agtaacgatg tcactatttg
    27721 tttgtatagc tcgatcaacc attttttcta tcattttagg atctaaataa tcatcactat
    27781 ccacaaataa atagtagtca cccgtagcct tttctaatgc ccgatttcgc gtagtagaaa
    27841 tacctgcatt ttcatagcta tatacatgaa tgaaatcata cttacttgca tataaattcg
    27901 caatttctaa agatgaatcg gttgttccgt cgttaataat aataatttct atatttttgt
    27961 atgtctgtaa aagaatagat tccagacatt ttgataaata tttttccaca ttatatactg
    28021 gaaccaatat actcacttta tacatgaacg gcctccttga atgccacaat acaatatccc
    28081 ttagataaaa aaatatcaaa tttacatata ataatcaata aaaatcaaaa aaaaaggacc
    28141 cggcagctag ctatcttcgc aggggcaagc ccaactattg tcgccgtgaa agtgcttaac
    28201 ttctgagttc gggatgagtt caggtg
//
LOCUS       DBMDWL010000004        27736 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_658_length_27736_cov_2.837489, whole genome shotgun sequence.
ACCESSION   DBMDWL010000004 DBMDWL010000000
VERSION     DBMDWL010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 27736)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27736)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27736
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_658_length_27736_cov_2.837489"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="ACZPDN_00505"
     CDS             complement(<1..292)
                     /locus_tag="ACZPDN_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297139.1"
                     /translation="MTESPDLSNTDNLILDGQRLILSSGTNFVNGAKYRTEIEGYSDI
                     TYKIKNAHAYLEVRTRDGRVLEYGSTDDSSVEALQSGPILFWLLSKVTDKNGN"
     gene            367..1005
                     /locus_tag="ACZPDN_00510"
     CDS             367..1005
                     /locus_tag="ACZPDN_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR1297140.1"
                     /translation="MLKQYWDMVTLLVAVLNHNNVVDKEYHDDISFYTQQMYLQLQKD
                     YPKYGITDEDIEIVTHLAPIHDIGKVRVPIEILNKPGKLTKEEMDIVKQHPLVGAEMT
                     QRFPKGLTTEKLNQYSYEICRHHHERYDGTGYPDGLKGEDIPLSAQVVGLVDAYDALI
                     SVRPYKRKLEHDQAVQMILNGECGKFSDELLHCFKKVSSQNNWIKRSEREAV"
     gene            1053..8795
                     /locus_tag="ACZPDN_00515"
     CDS             1053..8795
                     /locus_tag="ACZPDN_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HIR1297141.1"
                     /translation="MTTFITQIHMVSAEETDDTPTEEVVQETTPETSEVQEVQDVPQT
                     TENLDEYYKDGKILIYNYDQLKQIGSDAFVYTEDKDGNIGTGDIVVNEGTQLTYGKDA
                     QYLLMNEIKMDTKSIWNVKDDFTGKIEGEEIKEDETPALYDKKTDTIYIYNPYQLMVL
                     EQEDSDKEPVMSLDYDAPQFGMGQMIYPNGEDKDYLTYSKDHNYVISKNFSSDKPELV
                     ADQLSIDPDTTDTEGRDFKGQVVKTIGDKTYILIGNEDQLRAIGTDKAVQGAVYQAYL
                     ELGKWKIDKDSSGNSIMLYGGDADLTKEQNGIKDFTFGEVSDTDKKLSSVSSFHTRGK
                     CGVDQKTGKVDPNLDIKDQTNQKYTKNANYIIFRDIDLGSSNWTPLTFQGTMIGAKSA
                     EKGGLWKDGVINTIDKPVISNVTVNQSTKINVGEQMGAGFFSTISSEVSEKDIGLSKG
                     LVKVSNIQFDNLTVHTTTNETYYPPSLVSTLTNTLGKLLGGLLSGLTFILTFGQVKID
                     LGKTLGDILDARKKDPTALSTGAIAGRVEGQVEISNIEVNNANVKNVNGYTGGFVGYA
                     VGSTQYDGLSNALGDLVKLLTNILNVIPGLGLGDLIKILLGNAIPLGKLIPTGYINPK
                     IVNCNVNGLTISTASDKEYAGGFIGMQKGAIVERCSVTGGSNTISGKNFVGGFVGLAR
                     DDVIEGTLSGALDIETKLPKMNTESLLLNCSLNNAVVSVSGESYIGGFAGGLANASTV
                     NCNVKSDNALTVTASGNYAGGFAGIASLGWAADLGKDDTKDNLLGGVVDLVVKLLSSN
                     PGATSSLLSLAGVNPSHILGCSVNASLQVSGKDYVGGITGRGNGAYIAQSNSDNLQKI
                     SYWKNKVYDAGSVQVIDISLTGLSSVKGNDYVGGIAGSMGTASVAGLLNTTLGVASYL
                     AFTVDNVHVNGAENGFTVTGNERVGGGFGDAIGGSITTVSINNLASTEGNNLVGGFIG
                     LSGPGDLAGADGGLTVNLLGLNYILKLNNLLSLGQAIEVKINKVNVNGVTNGFTVHAK
                     GSRDSNSVRDYSASGFIAKSGSTKVEGSHVTNLKSVKAADDGGYASGFVAISKTGGLA
                     DVADDNSIKSLIEANGLVNAVGYLIPKYTNCTVSFVNGGSVTADVAGGFAADFQSGTV
                     DNSSRGENDYYAVYNLDSVNGQSYAGGFGGNVYSGALADAGKGISVLGNINGLKINIG
                     ELLNLVNAYVPIIKYAGVKSDNGFTVSANKIKSDDTNSGSAGGFIGYGSGVQVSHCDV
                     TNLKHTKVTPPKDLEGEDSSNYFGQESTYAVTGARYAGGYIGYMNIGSAASVGKGLSV
                     LGNAIGLNDVLDALNVVVSTIEHSNVTGRPGGFAVKASMKNTASDASKDDVLGDAGGF
                     AGKISGGHIQNSNSYNFSYIIGQITAGGYVGDLQSGSVADALGGTSILGGLVNTKETL
                     SSLAQDFVPTIRNSSTTCIPCGGAVRANASSTTTTQRGMAGGYAGHNEGGHIWGINTK
                     KWKGQDYKGPTSTCKAVRIRSVFGEEIAGGFTGLMESADTASAGSLSLLWGLVKADNI
                     LGALSVVYPTEENTAVYGPLALMDLSTWNSWVEYVGKYGGYGSDLAQNGKVDSQDKLN
                     EIIGQYVYGYNVVAGRTDYQDETILSNGGAAGGYVGSMLTGTITSGQAHDTKLVKGLR
                     CAGGFAGEMINGGAANLGGTSILGLKLELGQMLEVLNVFVPVIKQSSVEGYQSGLMVQ
                     SNGNNDKDLLGYAGGYVGKLIGGQIWGEEHSRCKVTKLRRVDGKSYVGGFVGNAKPGS
                     VATVDPVRGEGLFLTKLLQKLLETPTDLINVLNATVATIRYADVEAWNDWGVIVNGAY
                     DSGGNNTSYAKAAGGFAGMLEGTVLGKKDVAQAGVTVQNVRSVVSGEYAGGCFGIADV
                     SGVANISAGSETSLLDKLLKLGRADVLDAFRSYVYYGNVRGSKDAGLSVSANVANKYG
                     QNNEVTYSGTAGGFGGSLLNGSVKNSAVTNLSNVRGLNSVGGFVGYSGKSGVVGADKI
                     DVLGNNTGQLLGGDLGVMDIFGSHIDDSTVTGINGGYTVQSTGGVEQIAGGFIGYANL
                     ARISNCTAGAEDSSFGLKQVTSDGTAGGFAGRTSYAYLADVKLDSGPVNVLLAVVNKL
                     VQALYLDKIENSNLLKINLGLIKVDALYEGNLIHVNILGLDIKVGLSKKSKDNDQQTD
                     LAIITIGDSSIKLPCDKDGIKKDGDTKSNISLSLIKANRTKITGSQVYGISTGYDVYA
                     GGADNNKDGTAKDGRSGGFVGFNDEGFLKNNDMYLCDVVRGTSKLVGPFSGKSKLDSV
                     YKFNTQANVEGKKNNYRIYRKLEKKLDEIIKNTKKLNMQYSKDDSTGWGIYTLGHMNT
                     VQSYDNLQNAQITDSNGSAKVDLNAYESEAKAILMSDTKTNLNTGESDTPEPSESQDP
                     CDENIKLTINKVWKDFNNFSGIRPNSITVTISRTWTDADNKEHTETVPKYESYKIYGE
                     INKSTWKEVIEELPAYKTDEDGKTIHYYKYSVTEVEVNGYTTEIKSSEDGFTFTITNK
                     HFPGLPDTGGNGRYLIYLIGLLLLAIYLVTGKKRNKKNQLAE"
     gene            8831..10402
                     /locus_tag="ACZPDN_00520"
     CDS             8831..10402
                     /locus_tag="ACZPDN_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HIR1297142.1"
                     /translation="MKRIKKFAAALLTAIMMMTMTVTAFAAEPTGTLTVNVKGGQTLA
                     NQTVNVYKLFDMTVSGSGENLNYGYKVNDTYKSILDKVLGAEFTDKSDQGYYDAVQKA
                     TSDGKNGNNTQKFANDFTKELLNYNKSNTSAKIKATVTKTFDKASTDTSTTFGGLAYG
                     YYLVCQTGTKAIQSSLVALTESTATVNLKSEAPKIEKQADKATVFVGETVTYTVTGQV
                     PDTTGYDNYIYKIHDTLTSGLDFVTNTEGTKVKVTVKIGGTTVDVSEADFVNEAGEVV
                     TTPTRTMVLDLSEKVRTATVGQSIEVTYQAKVNKDAVVETKNSATLEYSNNPGTNGTG
                     TTKPSEVVTPTYPLDINKIKKGTENILAGAHFTLYGSTSEGRIDRTKAIKVTKDGEGK
                     YTYASDQNATENTTTDMVTVADKIDGKGNLHINGLAAGTYYLEETKAPDGFNKLTAPI
                     EIKITKSGDTDVNNWTISKDGKDESDKVIDIENSTGSLLPSTGGTGVIVFAGVAILLV
                     FGVVVSFIRDKRKAC"
     gene            10468..11331
                     /locus_tag="ACZPDN_00525"
     CDS             10468..11331
                     /locus_tag="ACZPDN_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1297143.1"
                     /translation="MSNKKRKMTIKDIIRLLVLVVAFAVLLYPTVSSYVNEKNGSKVV
                     SNYDAESVRLSNAEKEKMLEDARAYNKEMLSNIDLIDPFSQGEKSLDARYESLLNMDG
                     SGMMGYIRIPKIKVKIPIYHGTSESVLQAGVGHFWGTSLPVGGKSTHTVLTGHRGLPT
                     KTLFTNMDKLVKGDVFYIKVLDETLAYKVDQILTVLPEETEALSIVPGQDYATLVTCT
                     PYAINTHRLLVRGHRIPYKEAVKIEKNTSTGIELSFTTKVLIVTLGIIFIGLVIAMLY
                     SLYDKRRRKKK"
     gene            11328..12014
                     /locus_tag="ACZPDN_00530"
     CDS             11328..12014
                     /locus_tag="ACZPDN_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297144.1"
                     /translation="MIKQLKIHSLFLVLISLLFVSLIQVHADSRTGSIDIDYKGRTDN
                     QEEIILSGAKFEIIPIQYVENEKWVWQSSFVDCGVSLHDTSAEARNKQAKQLLEYARK
                     KGISGNQQLTDSMGHTVFSNLNEGMYLITQIGSLKDGNDTFESAPFLVSVPSDIDGYL
                     MYDVKVEPKVTWLTNNVPPSVPEKPSEKNPPEDTNTGVQVKKMTWIVLTLCSFGMIVI
                     LGRRLKENSY"
     gene            12399..12554
                     /locus_tag="ACZPDN_00535"
     CDS             12399..12554
                     /locus_tag="ACZPDN_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297145.1"
                     /translation="MIDDTLSHEEIIQLLTDRILDGDTDINMKSDGFLDSDIEEAHLN
                     CLALYFK"
     gene            complement(12606..13094)
                     /locus_tag="ACZPDN_00540"
     CDS             complement(12606..13094)
                     /locus_tag="ACZPDN_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297146.1"
                     /translation="MKKNLTEEIIKAKQECVNNDKITHVKKIRALAHEYISTNKADST
                     PEIKLDSHMSVRFHKGESDTPESVYITVNGKSYAGTEMDAIICKLDRIGPEYKNDKKK
                     DERNDSIKTKGRQIARVVQVDLVHKTVNLPMKSAAEGNSLLTSLLRLLGRTIKHSVQI
                     NR"
     gene            13463..14479
                     /locus_tag="ACZPDN_00545"
     CDS             13463..14479
                     /locus_tag="ACZPDN_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297147.1"
                     /translation="MKKKIGMRGTCVLVNTFNYALELINSKISRADLFEEALIYVDKN
                     QYIDFVELQRMNLNKDESVLVAENITFEVEDTLYDRCVKRVKEFFPDTRITHPFVVRM
                     ILNAYISYLEEKIELEKRLIPNEEASYVTSSGKRILKAVDGYGDERKKKAEEQSNRTP
                     LSFSKVILHVNELMDKISGKCTSRIDYLELDGKQNIVIDNVIVAVGLEWQDPVYDKIA
                     GYYTYYDHKYEKISKEFNSIDASDLIYVRFTKDGYIGVVGDSFDINFDMQTSSGKLLH
                     HVNKEWNKDIVLIIPLPSTITNPNYYERKNIRKKIETALGEYLIEKGNPIIDFYSHNN
                     FVEN"
     gene            complement(14570..15466)
                     /locus_tag="ACZPDN_00550"
     CDS             complement(14570..15466)
                     /locus_tag="ACZPDN_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297148.1"
                     /translation="MGQWSNRRKRRKTKRLTVQVYDRLNSMLQAGMGRSRKEDKKNGI
                     DYNFIYSTKTYETYKIECQHYVRWCKEHYPGAESIDDLKPYINDYLQHEIDRGMSAYT
                     ISTRKMAICKLFGIRATEIMQTPTRRRSDIKRSRNNVKRDSHISAKTEAKLARVTSCT
                     GLRRRELMMITGTALTYRDGVPYLHVTAGTKGGKTRDVEICGDIQEVAEIIAMFKAAG
                     PMRVLPKIHNAFDNHHYRAIYAQKMLKKYSRPIDTLSPSERYVMRRDHAGEILDRRAM
                     YIVSKNLGHNRIDVIAQSYIYY"
     gene            15883..16311
                     /locus_tag="ACZPDN_00555"
     CDS             15883..16311
                     /locus_tag="ACZPDN_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297149.1"
                     /translation="MKRTEFREAEENLIWKWVHEELPKQFGFDTDDTIGHETVIRFQC
                     YEGGKSWIFENDGDELELGLGRDGMFTATLSDITNKISVVSLNVLCIGDIEEIKEVVY
                     RLCDIFTTSGDWHSLMSFVPSITEIDTLKKCIEYYDELHR"
     gene            16429..16707
                     /locus_tag="ACZPDN_00560"
     CDS             16429..16707
                     /locus_tag="ACZPDN_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297150.1"
                     /translation="METVCLLSREKVDGYVNVDLDANKIVRKSKTGTATYKEIKEYIN
                     DKYGFTVSSLNIAQIKDKCGLEKRNNYNKGVDGHIKYLHVLQRKKKPF"
     gene            complement(16773..17108)
                     /locus_tag="ACZPDN_00565"
     CDS             complement(16773..17108)
                     /locus_tag="ACZPDN_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297151.1"
                     /translation="MDEKIFEKQFIERLTQLRYQKNVSARDLSLSLGLNAGYINNIES
                     GKALPSMGTFFLICSYLNISPMEFFDYDSPLPEELREAITNLKKLNPVQFTSIKLIIE
                     DLAKQSKNK"
     gene            17375..18400
                     /locus_tag="ACZPDN_00570"
     CDS             17375..18400
                     /locus_tag="ACZPDN_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LptM family lipoprotein"
                     /protein_id="HIR1297152.1"
                     /translation="MTKNSIMRKLFSIAMCAILVLSLSACGSKKENVSNKDENTANSA
                     ASSEKDNQDIKKNVVKVDDLSVNPITIVGSDHSAKFGELEGTVIEATIKNKSNDSVDV
                     SLVVEATVEYIDDYEDKQTVNMLMYNPSKSFGNNATSFHSPYTRYNVYEANNSDIPVD
                     GLIPVGLAPKEEKTVRYYFGENYFYGYDLDNDNEPMEIKGGTITKIKLKDVNCSVPDC
                     EYVAVDEWEDCVELVNENTHNNEYDSSMLAYLSGVIKNNTEERWKQATVQMDAYVDDQ
                     EYYEVIKSTGSTIGIDHDFSLIFDRALRYPETSDYGSCSYIEYDSNDEQKIEVTPALM
                     YYIPEGK"
     gene            18664..18858
                     /locus_tag="ACZPDN_00575"
     CDS             18664..18858
                     /locus_tag="ACZPDN_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297153.1"
                     /translation="MDERMLEYGHSCSSQDIYETVHHDAVYETQRVKTGTKTVVDKAA
                     WTETVVTGQKCSSCGATKPE"
     gene            19174..19599
                     /locus_tag="ACZPDN_00580"
     CDS             19174..19599
                     /locus_tag="ACZPDN_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297154.1"
                     /translation="MKKTILKRSVASTLSFLMAFSTAVPNSVVYAEESGIESEIAQDY
                     AATLGQTQNGTTSFVTDGNKTKDPTTHVQGDKVEVAVEPDEGFNTAKIEVKNTDGTVV
                     ASLEDGKVTANDEGNYTFEMPASDVTVSVSFETAKKRSG"
     gene            19571..19726
                     /locus_tag="ACZPDN_00585"
     CDS             19571..19726
                     /locus_tag="ACZPDN_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297155.1"
                     /translation="MKPQKKEAADAETETTRSETETEKNETEEVKEEVKANIFDEFFD
                     KYKSSCT"
     gene            20130..20387
                     /locus_tag="ACZPDN_00590"
     CDS             20130..20387
                     /locus_tag="ACZPDN_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297156.1"
                     /translation="MIGTYDIGDGFYVDIVLDRETQMYDYWLYNKDYDIKIVCIKLPK
                     YPDYKGFKAEMIKNCRDVERYLDYILSENYKTYYRDKYMQD"
     gene            complement(20688..21707)
                     /locus_tag="ACZPDN_00595"
     CDS             complement(20688..21707)
                     /locus_tag="ACZPDN_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297157.1"
                     /translation="MIKIRNTLYENGDAEIKITPFQYYPNNKGTVRSDEDRISARNRQ
                     RKKRAKDHAMSFKATNFLTLTAPEDSPLRTDINTFMKLADKFLATQEVNYFLYPQFYR
                     GTNEYHIHGLSNGAIDFKKWIELTNANPNNCYCKPLEKSQLDAAEYIIRDYGKMPKGL
                     RPRVSNVKACKAKSSFQIINDETGEILSDTSCGSIPNKNQFKYYKEYINNTQNHNDNS
                     NAYDSTDSLYNYDENYELINNDSCILTSNKNEIIDTLNVDMYNNNESRNCHFSSRTII
                     LTFFIFLCSFYTIYKYLKAMFSLGVYLFCNMKTSENESLFIPLPYLFLFTGKVCPYDS
                     NAPPS"
     gene            22287..22469
                     /locus_tag="ACZPDN_00600"
     CDS             22287..22469
                     /locus_tag="ACZPDN_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025565.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HIR1297158.1"
                     /translation="MEKMIMEKMMNTKIEGNTFTKKIGQTFYVVRYHFNEDAKETLQE
                     KINRMLVTEASRMTVY"
     gene            22542..22733
                     /locus_tag="ACZPDN_00605"
     CDS             22542..22733
                     /locus_tag="ACZPDN_00605"
                     /inference="COORDINATES: protein motif:HMM:NF039323.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6019 family protein"
                     /protein_id="HIR1297159.1"
                     /translation="MWNELGISGGTAIVILIALYFVIKWAVKNGIKEAYSAITGKKTD
                     EDIQNEKVLKELGLDSEDQ"
     gene            22761..22940
                     /locus_tag="ACZPDN_00610"
     CDS             22761..22940
                     /locus_tag="ACZPDN_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297160.1"
                     /translation="MEKDKKEKIRCICFLFVSICNFIVSIVNFMDKNTTQAVTYLLLG
                     ISLLLLSTTNLRKNK"
     gene            22999..23202
                     /locus_tag="ACZPDN_00615"
     CDS             22999..23202
                     /locus_tag="ACZPDN_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297161.1"
                     /translation="MKKTSLVAVILVGLAAVIWTIRAVLEIVLKTYNESVFLFVLNVL
                     CSVVWIVSFIVNLKRYRSNRENK"
     gene            23357..23917
                     /locus_tag="ACZPDN_00620"
     CDS             23357..23917
                     /locus_tag="ACZPDN_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013213.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1297162.1"
                     /translation="MYVAVNNAEIRNCNLKKRLHSLCQRTLRSQVTEYVRYTNRILIL
                     QEYTPSADEEKLYNFISDYLQTDKLYALPEGQRTLITMVLRKLLASSSFAISGTLNSL
                     IERLNNLLSDVALSRGYYFSINHQMIKNQKGRNIINRIPIEKILIESDAPFTIGLNSD
                     YNIPFANELIDFLSLSRNIDKTVLYM"
     gene            24287..24997
                     /locus_tag="ACZPDN_00625"
     CDS             24287..24997
                     /locus_tag="ACZPDN_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1297163.1"
                     /translation="MSKIYGYVRISTKKQSIERQIKNILQLYPTANIKSEIYTGRRTE
                     GRAVWNLLYKEIKTGDTIVFDEVSRMSRNAKEGFKLYKELFAKGVNLVFIKEPHINTD
                     TYRNAMTSTINVDVNTADENAAKLLNDIFDALNSYMFRLAEESIRLAFDRSQAEVDYL
                     SQRTIEGLNLARENGKQIGNTAGSKRTTQKSIKAKELIKKHSKNFGGSLTDGEVMQLA
                     KCTHNTYYKYKRELLQEI"
     gene            25187..25345
                     /locus_tag="ACZPDN_00630"
     CDS             25187..25345
                     /locus_tag="ACZPDN_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297164.1"
                     /translation="MVFDIYDFITDYVKKANKVLEIEESQIVFYSVDPDDDEPLVKYC
                     NHVIFIKN"
     gene            25539..25760
                     /locus_tag="ACZPDN_00635"
     CDS             25539..25760
                     /locus_tag="ACZPDN_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020602.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HIR1297165.1"
                     /translation="MEGTKEKIDVRIAERPFISPKEAAAYFGIGINTVRHFANQHQET
                     ILWVGNKMMIKRRKMEAYLENAYELETPK"
     gene            25860..27044
                     /locus_tag="ACZPDN_00640"
     CDS             25860..27044
                     /locus_tag="ACZPDN_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1297166.1"
                     /translation="MKKKVTKDGKRLKDCENIRKDGIYQYRYRDASGKYKYLYSKSLS
                     ALRIKESRIESELIAGINPMDADMTVMQLLEKYISIKDGVRYNTKVGYQFVMNVMEKE
                     GFSERKIRDIKVSDAKAWLVKLQNDGRGYSSITSIRGVIKPAFQMAFEDDLIVRNPFA
                     FQLKGVIRNTTRERKPLSLEEQKMWLDFMRTDSTYSKYYDDMVILLETGMRVSEFCGL
                     TVSDVDFENNCIYLNKQLLRTRNGVLFVEETKTSCGRRKVPMSPAVREAFSRLITKRK
                     KLGKGVSVDGFKNFILFDKNAKPKVALHVENEMRWGLKKFKKLYPEMEYLEVTPHILR
                     HTFATNNILYGKMGIKGAKLVMGHSEAHVTMDVYTGSDYSQAAKEIVSVSNLFDESSK
                     EF"
     gene            27205..27666
                     /locus_tag="ACZPDN_00645"
     CDS             27205..27666
                     /locus_tag="ACZPDN_00645"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR1297167.1"
                     /translation="MSKIKVLFICHGNVCRSVSAEYIMKDLAKKNGVDLYVSSAATSR
                     EEIGNDIYPPAKRKLKEKGIPFSRHYARQATMSDYLSYDYLICMDENNIRNLNRMFNN
                     DPDHKIYKLLSRDVSDPWYTNDFEMAYNDIFEGCKAWLEKLSWCASSVQLI"
BASE COUNT         9582 a         3783 c         5700 g         8671 t
ORIGIN      
        1 catttccatt tttatcagtc actttagata acaaccagaa aagaataggc cctgattgaa
       61 gggcttcaac agatgaatca tctgtcgaac catattctaa gactcttcca tctctcgttc
      121 ttacttctaa ataagcatga gcattcttta ttttataagt gatatcacta taaccttcta
      181 tttctgtcct atactttgca ccattcacaa aatttgttcc agaactcaat attaaacgct
      241 gtccgtcaag aatcaagtta tcagtattcg ataaatctgg tgattccgtc attccatcat
      301 aataataaga ttttccaaaa taccattgta agtatggagc aggaattaag aaataaagag
      361 aaagtcatgt tgaagcaata ttgggacatg gtaactcttt tagtagcggt attaaatcat
      421 aataatgtag tggacaaaga atatcacgat gatattagtt tttatacgca acagatgtat
      481 cttcaacttc aaaaggatta tccaaaatat gggattacag atgaagatat tgaaatcgtg
      541 actcatttag cccctattca tgatattgga aaggttcgtg ttccaattga aattttgaat
      601 aagccaggaa aactaacaaa ggaagagatg gatattgtaa aacagcatcc tttggttggt
      661 gctgagatga cacagagatt tccaaaaggt ttaacaacag aaaaattgaa tcaatatagt
      721 tatgaaatct gtagacatca tcatgaaagg tatgatggta caggatatcc tgatggatta
      781 aagggtgaag atattccttt gagtgcgcaa gtcgtaggtt tagtagatgc ctatgatgca
      841 cttattagtg ttcgtcctta taagagaaag ctagaacatg atcaagccgt acaaatgatt
      901 ttgaatggtg agtgtggcaa gttctcagat gaattactgc attgttttaa aaaggtttca
      961 agccaaaaca attggataaa aagaagtgaa agggaagctg tatgagaagg ataagaaagt
     1021 taacatccaa ttttcttgta tgcattcttt gcatgactac ctttattaca cagattcata
     1081 tggtaagtgc tgaagaaaca gatgatactc ctacagaaga ggttgtacaa gaaacaactc
     1141 cagaaacaag tgaagtacaa gaagtacagg atgttccaca aacaacagag aatttggatg
     1201 aatattataa ggatggaaag attcttattt acaattatga tcaattgaaa caaataggtt
     1261 cggatgcctt tgtctataca gaagataaag atggaaatat cggaactgga gatattgttg
     1321 taaatgaagg gactcaactt acttatggta aggacgctca gtatctattg atgaatgaaa
     1381 tcaaaatgga tacaaaatca atttggaatg taaaggatga ctttacagga aagatagagg
     1441 gagaagaaat caaagaggat gaaactcctg ctttatatga taagaaaaca gatacgattt
     1501 atatttataa tccatatcaa ttaatggttc tagaacaaga agattcagat aaagagcctg
     1561 taatgtcatt ggattatgat gcaccacaat ttggtatggg acaaatgatc tatccaaatg
     1621 gggaagacaa agattattta acttacagta aagaccataa ttatgtcatt tcaaagaatt
     1681 tcagttcaga caagcctgaa ttagttgcag atcaattgag tatagatcct gatacaactg
     1741 atactgaagg acgtgacttt aagggacagg ttgtaaaaac aattggtgac aaaacgtata
     1801 tcttaattgg aaatgaagat caattaagag caataggtac agacaaagca gtccaaggag
     1861 cagtttatca agcctatctt gaactaggaa aatggaaaat cgataaagat tcatctggta
     1921 attcaattat gttgtatggt ggcgatgccg acttaacgaa agaacaaaat ggaataaaag
     1981 attttacgtt tggagaagta tcagatacag ataagaaatt aagtagtgta agtagttttc
     2041 ataccagagg taaatgtggt gttgatcaaa aaacaggtaa ggttgatcca aaccttgata
     2101 tcaaggatca aacaaatcag aaatatacta aaaatgcgaa ttatattata ttccgtgaca
     2161 ttgatttagg cagttcaaat tggactccac ttactttcca aggcacaatg attggtgcta
     2221 aatctgcaga aaaaggcggg ctttggaaag atggtgttat aaacacaatc gacaagccag
     2281 taatttcaaa cgtaactgta aatcaatcaa ctaagattaa tgttggtgaa caaatgggtg
     2341 ctggattctt ttcaacaata tcaagtgaag taagtgaaaa ggatattggt cttagtaaag
     2401 gattggtaaa ggtatctaat attcaatttg ataatctaac agttcataca acaacaaatg
     2461 aaacctatta tccaccttca ttagtaagta cacttacaaa tactctagga aagttattgg
     2521 gtggattgtt gagtgggtta acatttattt taacttttgg tcaagtgaaa attgatttag
     2581 gcaaaacatt aggtgatatt ttagatgcac gtaaaaaaga tcctacagct ttatcaactg
     2641 gagcaattgc tgggcgagtt gaaggacagg ttgaaatttc aaatattgaa gtcaataatg
     2701 caaatgtaaa aaatgtgaat ggctatacag gtggttttgt tggatatgcc gttggttcaa
     2761 ctcaatacga tggattatcg aatgctttag gggatttggt taaattatta acaaatattt
     2821 taaatgtcat cccaggttta ggcttaggtg atttaattaa gattctatta gggaacgcaa
     2881 ttcctttagg aaagttaatt ccaacaggat atatcaatcc gaagatagtg aattgtaatg
     2941 taaatggttt aacgatttca acagcctccg ataaagaata tgccggtgga tttattggta
     3001 tgcaaaaggg tgctattgta gagcgttgtt ctgtaacagg tggttcgaat acaattagtg
     3061 gaaagaattt tgtaggtggc tttgttggtc tagctcgtga cgatgtgatt gaaggaacgt
     3121 tatctggggc attggatata gagacaaaac ttcctaagat gaatacggaa agtttgcttc
     3181 taaattgttc attaaataac gcagttgtgt ctgtttctgg tgaaagttat attggtggtt
     3241 ttgccggagg attggccaat gcatcaactg tcaattgtaa tgttaaatcg gataatgcac
     3301 tcactgtaac ggcttctgga aattatgctg gtggttttgc aggaattgca tctcttggat
     3361 gggctgctga cttaggtaaa gatgacacaa aagataactt acttggcggt gtagttgatc
     3421 ttgttgtaaa acttttaagt agtaatcctg gagcaacttc atctttactt tctcttgctg
     3481 gtgtaaatcc atcacatatc ttaggatgtt ctgtaaatgc ttcacttcaa gttagtggta
     3541 aggactatgt tggtggtatt acaggtcgag gcaatggtgc atatatagct caatcgaatt
     3601 cggataattt acagaaaata tcatattgga aaaataaggt ttatgatgct ggttctgttc
     3661 aagtgattga tatctcttta actggattat ccagtgttaa aggaaatgat tatgttggtg
     3721 gaatcgctgg atcaatgggt acagctagtg tggctggttt attaaataca acgcttggtg
     3781 ttgcatctta tcttgcattt actgtagata atgttcatgt aaatggtgca gagaatggat
     3841 ttactgttac gggaaatgaa cgtgttggcg gtggatttgg agatgcgatt ggtggttcga
     3901 taacaactgt tagcatcaat aatttagctt ctactgaagg caataactta gtaggcggat
     3961 ttataggtct ttctggtcct ggcgatttgg caggagcgga tggtggattg accgttaatc
     4021 tattaggttt gaattatata ttgaaattga ataatttgct ctctctagga caagcaattg
     4081 aagttaagat taataaagta aatgtgaatg gagtcacaaa tggatttacg gtacacgcaa
     4141 agggtagtcg tgattctaat tctgttcgtg attatagtgc ttctggtttt atcgcaaaaa
     4201 gtggaagtac taaagtagag ggttctcatg taacaaatct aaaatcagtg aaagctgcag
     4261 atgatggcgg atatgcttca ggatttgtgg ctatttcaaa aacgggtggt ctagcagatg
     4321 ttgctgatga caattcaatt aaatcattga ttgaggctaa tggtttagta aatgccgtag
     4381 gatatttaat tcctaaatat acaaattgca cggtttcttt tgtgaatggt ggaagtgtta
     4441 cagcagatgt tgctggtggt tttgcagcgg atttccaaag tggaacagtg gataacagtt
     4501 cgcgtggtga aaatgattat tatgctgtat ataatttaga ctctgtaaat ggtcaaagtt
     4561 atgcaggtgg ctttggtgga aatgtttatt ctggagcatt ggcggatgct ggtaaaggta
     4621 ttagtgttct aggaaatatt aatggtttaa aaataaatat tggagaattg ttaaatttag
     4681 taaatgcata tgttccaatt ataaagtatg caggtgttaa atctgataat ggatttacag
     4741 tatcggctaa taaaattaaa tcggatgata cgaattctgg tagtgcaggt ggctttattg
     4801 gctatggtag tggagttcag gttagtcatt gtgatgtaac aaatttaaaa catacaaaag
     4861 taacacctcc taaagattta gagggtgaag atagttcaaa ttattttggt caggaaagta
     4921 catatgcagt aacaggtgca agatatgctg gtggatatat tggatatatg aatattggtt
     4981 ctgctgcttc ggttggaaaa ggcttgagtg ttttaggtaa tgcgattggc ttgaatgatg
     5041 tattggatgc attaaatgtt gtagtttcta caattgaaca ttctaatgtt actggaagac
     5101 ctggtggttt tgcggttaaa gcctctatga aaaacacagc ttcggatgct tctaaagatg
     5161 atgtattagg agatgctggt ggttttgcag gtaagatctc tggtggacat attcagaatt
     5221 caaactcata taatttctca tatatcattg gtcagattac agctggcggt tatgttggag
     5281 atttacagtc tggtagtgtg gcagatgctt taggtggaac aagtatatta ggtggtcttg
     5341 ttaatactaa ggaaacactt tcttctttag cacaggattt tgttcctacg attcgaaata
     5401 gttctacaac ttgtattcct tgtggtggtg cagttcgtgc aaatgcaagt tctaccacaa
     5461 caactcaaag aggtatggct ggtggttatg ctggtcacaa tgaaggcggt catatctggg
     5521 gtattaatac aaagaaatgg aaaggtcaag attacaaagg accaacaagt acttgtaagg
     5581 ctgttcgtat tcgatctgtt tttggagaag agattgcggg tggatttaca ggcttaatgg
     5641 aatctgcaga tacggcaagt gcaggaagct tgtctctatt atggggattg gtaaaagccg
     5701 ataacatctt aggagcatta agtgttgttt atccaactga agaaaacaca gcagtctatg
     5761 gtccgcttgc attaatggat ttaagcacat ggaattcatg ggttgaatat gttggtaagt
     5821 atggtggcta tggtagtgat ttggctcaga atggaaaagt agatagccaa gataagttaa
     5881 atgaaatcat tggtcaatat gtctatggct ataatgttgt agctggaaga actgactatc
     5941 aggatgagac gattctttca aatggtggtg cagctggtgg ctatgtgggt tccatgttaa
     6001 caggaactat cacaagtggt caagctcatg atactaagtt agttaaagga cttcgttgtg
     6061 ctggtggatt tgccggagaa atgatcaatg gtggtgctgc aaatcttggt ggtacaagta
     6121 ttttaggatt gaaattagaa ctaggacaga tgcttgaagt tttaaatgta tttgtaccag
     6181 taattaaaca atcttcagtc gaaggctatc aaagtggtct tatggttcag tcaaatggaa
     6241 ataatgataa agatcttctt ggatatgcgg gtggctatgt tggaaaactg attggtggtc
     6301 aaatctgggg agaagaacat tcacgatgca aagtcacaaa gcttagacga gtagatggta
     6361 aaagttatgt tggtggtttt gtaggtaatg caaagcctgg tagtgtggct acagtagatc
     6421 ctgttcgtgg agaaggatta ttcctaacta agcttttaca aaagttatta gaaacaccaa
     6481 ctgatttaat taatgtgtta aatgcaacag tcgcaactat ccgttatgca gatgttgaag
     6541 catggaatga ctggggtgtt atcgtcaatg gagcttatga tagcggaggg aataatactt
     6601 cttatgcaaa agctgctggt ggttttgcgg gaatgcttga gggaaccgta cttggtaaaa
     6661 aagacgttgc tcaggcagga gttactgtgc aaaatgttcg ttctgtagta tctggtgaat
     6721 atgccggtgg atgttttgga attgcagatg tttcaggtgt tgctaatatc agtgcaggta
     6781 gtgaaacgtc tttattggat aaacttttaa aactaggccg agcagatgta ctggatgcat
     6841 ttagaagcta tgtatattac ggaaatgtaa gaggaagcaa ggatgcaggt ctaagcgtga
     6901 gtgcaaatgt tgctaataag tatggtcaga ataatgaggt tacatatagt ggaactgctg
     6961 gtggttttgg tggttcacta ttgaatggtt cagtgaaaaa tagtgcagta acaaacttaa
     7021 gtaatgttcg aggtttaaac agtgtaggtg gttttgttgg atattctggt aagagtggag
     7081 ttgttggagc ggataaaatt gatgttcttg gaaacaatac tggacaactt cttggtggag
     7141 atcttggtgt tatggatatc tttggatctc atattgatga ctctacagtt acaggaatta
     7201 atggtggtta tactgttcaa agtactggtg gagtagagca aattgctggt ggatttattg
     7261 gctatgcaaa ccttgcaaga atttccaact gtacagctgg agcagaagat tcatcttttg
     7321 gtttaaaaca agttacatcc gatggtacag ccggaggatt tgcgggaaga acaagttatg
     7381 catatcttgc agatgtcaaa ttggattcag gacctgtaaa cgtattgctt gcggtagtta
     7441 ataaacttgt acaggcactg tatttggata aaattgaaaa ttctaatctt ttgaagatta
     7501 atctaggact tatcaaagtg gatgctttat atgaaggaaa tttaattcat gtaaatatac
     7561 ttggattaga tattaaagta ggattatcca aaaaatcgaa ggataatgac caacagacag
     7621 atcttgcaat cataacaata ggagattctt cgattaaact tccatgtgat aaagatggaa
     7681 taaaaaaaga tggtgatacc aagtcgaata tcagtttaag tttgattaaa gcgaatcgta
     7741 caaagattac gggcagtcaa gtttatggaa taagcactgg ttatgatgtc tatgcaggtg
     7801 gtgccgacaa taacaaggat ggcactgcaa aagatggacg aagtggaggc tttgtaggat
     7861 tcaatgatga aggtttttta aagaataatg atatgtattt atgtgatgtt gttcgtggta
     7921 cttcaaaatt ggttggtcca tttagtggaa agagtaaatt agattcagtg tataaattta
     7981 atacacaggc aaatgttgag ggaaagaaga acaactatcg tatctataga aaattagaga
     8041 aaaaattaga tgagatcatt aagaatacga aaaaattaaa tatgcaatat tcaaaagacg
     8101 attcaacagg atggggcatc tatacattag gacatatgaa tacagttcag agttatgaca
     8161 atctacaaaa tgctcagatt acggattcaa atggatcggc taaggtcgac ttaaatgcat
     8221 atgaatctga agcgaaggct atattaatgt cggatacaaa aacaaattta aatacaggag
     8281 aaagtgacac gcctgaacct tcagagtcac aggatccttg tgatgaaaat attaaattaa
     8341 caatcaacaa agtatggaag gatttcaata attttagtgg aattcgaccc aacagtatta
     8401 cggtaacaat ttctcgtact tggacagatg cagacaacaa agaacatact gaaacagttc
     8461 caaagtatga gagttacaaa atatatggtg aaatcaacaa atcgacttgg aaggaagtaa
     8521 tcgaagaatt accagcttac aagacggatg aagatggaaa aaccatacat tattacaagt
     8581 actccgttac agaagtggaa gtaaatggtt atacaacaga aataaaatct tcagaagatg
     8641 gatttacatt cacgataacc aacaaacact tcccaggatt acctgataca ggtggtaacg
     8701 gaagatactt aatttatttg ataggtcttc tgcttttagc gatttatctt gttacgggca
     8761 aaaaaaggaa caagaaaaat cagctcgcag aatagctata aaaaattcaa agaaaaggag
     8821 aatgtaggaa atgaaaagaa ttaaaaaatt cgcagcagcc ttgctgacag ccatcatgat
     8881 gatgactatg acagttacag cattcgctgc agaaccaact ggaactctta cagttaatgt
     8941 aaaaggaggt cagactttag caaaccaaac tgtaaatgtc tataagttat ttgatatgac
     9001 agttagtggg tcaggggaaa acttgaacta tggttataag gtaaatgata cttacaaatc
     9061 tattttagat aaggtgttag gagcagaatt cactgataaa tcagatcagg gatattatga
     9121 tgcagtgcag aaagcaacta gcgatggaaa aaatggaaat aacactcaaa aatttgcaaa
     9181 tgattttaca aaagaattat taaattataa caaaagcaat acgtctgcaa aaattaaagc
     9241 aacagttact aaaacatttg ataaagcttc aactgataca agtactactt ttggcggctt
     9301 ggcttatggt tactatttag tttgtcagac aggtacaaaa gctatccagt cttctttagt
     9361 tgcattaact gagtctacag caactgtaaa tttaaaaagt gaagcaccaa aaattgagaa
     9421 acaagcggat aaagcaactg tatttgttgg ggaaactgtt acatatacag ttacaggaca
     9481 agttcctgat acaacaggtt atgataacta tatttacaag attcacgata cattaacaag
     9541 tggacttgat tttgtgacaa atacagaagg aactaaggtt aaagttactg ttaaaattgg
     9601 tggtacaact gtagatgtaa gtgaagcaga ttttgttaat gaagcaggtg aagttgtaac
     9661 aaccccaaca agaactatgg ttcttgattt atctgaaaaa gtacgaacag caactgttgg
     9721 gcaatcaatt gaagttactt atcaagctaa ggtaaataaa gatgctgttg ttgaaacaaa
     9781 gaacagtgca acattggaat acagtaataa tccaggtact aatggaactg gtacaactaa
     9841 accaagcgaa gtagtaacac cgacttatcc tttagatatt aataagatta aaaaaggtac
     9901 tgaaaatata ttagctggag cacattttac tttatatgga tcaactagtg aaggaagaat
     9961 cgatagaact aaagctatta aagtaacaaa agatggtgaa ggtaaatata cttatgcatc
    10021 tgatcaaaat gcaacagaaa atactactac tgatatggta acagttgcgg ataagattga
    10081 tggaaaagga aacttacata ttaatggtct tgctgctggt acttattatt tagaagaaac
    10141 gaaagcacca gatggattca ataaattaac tgctccaatt gaaattaaaa taacaaagag
    10201 tggagataca gatgtaaata attggactat ttctaaggat ggtaaagatg aatcagataa
    10261 ggttatcgat attgagaaca gtacaggatc actactacca tcaacaggtg gaacaggtgt
    10321 tatcgtgttt gcaggtgtag ctattctatt agtgtttgga gttgttgtaa gcttcattcg
    10381 tgataaaaga aaagcttgtt aattaatggg gtacggttgt cgtttagatg accgcgattc
    10441 ccatataacg atagggggaa tgcaataatg agtaataaga aaagaaaaat gactataaaa
    10501 gatataattc gattgcttgt gcttgtcgtt gcattcgccg tgttgttata tcctacagtc
    10561 agtagttatg taaatgaaaa gaatggttct aaggttgtat cgaattatga tgcagaaagt
    10621 gttcgcttga gtaatgccga aaaagaaaag atgcttgagg atgcacgtgc atacaataag
    10681 gaaatgctga gtaatattga tttgattgat cctttttctc agggtgaaaa aagtttggat
    10741 gctcgatatg aaagcctatt aaatatggat ggatctggca tgatgggtta tattcgtatt
    10801 cctaagatta aagtgaagat tcctatctat cacggtactt ctgaatctgt attacaggca
    10861 ggagttggtc atttctgggg aacttctctt cctgttggtg gcaaaagtac gcatacggta
    10921 ttaaccggtc ataggggact tcctacaaaa acattgttta ccaatatgga caaacttgtt
    10981 aaaggggatg tgttctacat caaagtctta gatgagacat tggcatataa agtggatcaa
    11041 atacttactg tattaccaga agaaacagaa gctttatcaa tcgtacctgg acaagattat
    11101 gcgacattag ttacttgtac tccatacgca atcaatacgc atagactctt ggttagaggt
    11161 catcgtattc cgtataagga agctgtaaag attgagaaaa acactagtac aggtatagaa
    11221 ttatcattta ctacaaaggt attgattgta acacttggaa ttatttttat aggacttgtt
    11281 atagccatgc tatatagttt gtacgataag agaaggagga agaaaaagtg attaaacaat
    11341 taaagataca ttcgctcttt ctagtcctta ttagcttatt gtttgtctct cttattcaag
    11401 ttcatgcgga cagtagaacg ggaagtattg atattgacta taaaggaaga acagataatc
    11461 aggaagagat tatcttgtct ggagctaagt ttgaaatcat ccccattcaa tatgtagaga
    11521 atgaaaaatg ggtatggcaa tcttcatttg tggattgtgg tgtttcactt catgatacaa
    11581 gtgctgaagc tagaaataaa caggctaaac agttattgga atatgctaga aaaaaaggta
    11641 tttctggaaa tcaacagctt acagatagta tggggcatac tgtatttagt aatttaaatg
    11701 aaggtatgta tttgattaca cagattggtt ctttaaagga tggaaatgat acgtttgaat
    11761 ctgcaccatt tttagtaagt gttccatccg atatagatgg atatctcatg tatgatgtaa
    11821 aggtagaacc aaaggtgaca tggcttacaa ataatgtgcc tccttctgtt ccagaaaaac
    11881 caagcgagaa gaatcctcca gaagatacga atacgggtgt tcaagtgaaa aaaatgactt
    11941 ggattgttct tacattgtgt agttttggaa tgattgttat tcttggaaga agattaaaag
    12001 aaaatagtta ttgaaagacc attgattggt ctttttaatt ttggattagc gattaattgt
    12061 gtgatgatat atactttttg taaaagaaag tattaaaaat taagatataa gcagttattt
    12121 tagatgaaat atatgaactg tttttatctc tatgaaaatg ttgtaagtaa aggagttttg
    12181 actatgaaga aagtgctttt tatttgccat gggaggagcc ttaaataatt cgtaaaagct
    12241 tttaaaatag gggatttaga gcatatttta tgttcaacta cacagattca ctacacaaca
    12301 atgtataaga aaggccagat atgacaaaca gtgtgtagta aggaatcctt attgcgacat
    12361 ccttattttt aatgaactaa atgaaaggcg gaaattatat gattgatgat acattgtctc
    12421 acgaagaaat aattcagcta ctaactgacc gtattctaga cggtgatact gacataaata
    12481 tgaaaagtga tggcttttta gattcagata ttgaagaagc ccatttaaat tgtttggcac
    12541 tatattttaa ataagcaaaa gatgatatcc aattgcgatt caattgtgat accatctttt
    12601 atcttttaac ggttaatttg tacgctatgt ttaatggttc taccaagtaa acgcaaaagg
    12661 ctagtaagta gggagttgcc ttcagctgca cttttcattg gcaggtttac tgttttatgc
    12721 acaagatcaa cttgaacgac acgggcaatt tgacgacctt ttgtttttat gctatcattc
    12781 cgctcatctt tttttttgtc atttttgtat tcggggccaa tacgatctaa tttacaaatt
    12841 attgcatcca tttcggttcc tgcatacgat tttccattta cagtaatata aaccgattct
    12901 ggcgtatctg attctccttt atgaaagcgt actgacatat gactatctaa tttgatttct
    12961 ggggtactgt ctgccttatt tgttgagata tattcatgtg caagagcacg tatcttttta
    13021 acatgcgtaa ttttatcatt atttacacat tcctgttttg ctttaataat ttcttctgtt
    13081 agattctttt tcaatttaaa caccttcctt ttggctattg gatattagga gaacaagaga
    13141 agcccggaac gcttccctca atcttttgta catttggttc tccttagatc agaaaagcga
    13201 acacccttat aaggagaaaa ctaataaatt tgatgaaagg agtttgtatc aaatgtagtg
    13261 ctccagcttt ctaatctata cattatgtat gtcagataaa gtgttcggaa agaaaaaaat
    13321 agccaaaatt aattatatgg ggtatatgct tcttattttt atatgaaaat atataaaatg
    13381 atgtcattga ctaacaatgt tagttgtgat atctttatta taaactaaca ttgttagttt
    13441 gaggcattgg aggttatatt aaatgaagaa aaaaattggt atgcgcggaa catgtgtatt
    13501 ggttaatact tttaattatg ctttagaatt aattaattca aaaatctcta gggcagattt
    13561 gtttgaagag gcattaatat atgttgataa aaatcaatat attgattttg tagagttgca
    13621 aagaatgaat ttaaacaaag atgaaagtgt actcgtagca gaaaatatta cttttgaggt
    13681 ggaagataca ttatacgaca gatgtgttaa acgtgttaaa gaattctttc cagatacgag
    13741 aattacacat ccctttgttg ttagaatgat tttgaatgcg tatatttcat atctagagga
    13801 aaaaatagag ttagaaaaac gacttatccc taacgaagaa gcatcgtatg tgacttcatc
    13861 tggtaagaga atactaaaag ctgttgatgg atatggggat gaacgaaaaa agaaagcgga
    13921 agagcaatca aatagaactc cattaagttt cagtaaagtg attttacatg tgaatgagtt
    13981 aatggataag attagtggta agtgtacttc aagaattgat tatcttgaac ttgatggaaa
    14041 acaaaatata gtaatagata atgtgatcgt tgcagtaggt ttagaatggc aagacccagt
    14101 gtatgacaaa atagctggtt attatacgta ttatgaccat aagtatgaaa agataagtaa
    14161 agaatttaat tcgatagatg cgtctgatct tatttatgta aggtttacaa aagatggtta
    14221 tattggggtt gtcggagatt cttttgatat taactttgat atgcagactt cttctggaaa
    14281 acttttacat catgtaaata aagaatggaa taaagatatt gtattaatta ttccacttcc
    14341 ttctactata acgaatccga attattatga aagaaaaaat ataagaaaaa agattgagac
    14401 ggcattaggg gagtacttaa tagaaaaggg gaaccccatt atagattttt atagccacaa
    14461 caactttgtg gaaaattaaa aatgactaaa aacaaaaagc aaatctattc tgtgcataga
    14521 tttgcttttt gtttttacat caaagggagt tcaaattatt ctagaaaaat taatagtata
    14581 tatacgattg agcgattaca tctatcctgt tatggcccag atttttcgac acgatataca
    14641 tcgctcgacg atctagaatt tctccggcgt ggtcacgtcg cataacatag cgttcagagg
    14701 gactcagggt gtcaatcggt ctgctgtact tttttaacat tttttgagca tatatagccc
    14761 tatagtgatg attgtcgaaa gcattatgaa tttttgggag cacacgcatt ggacctgctg
    14821 ctttaaacat tgcaatgatt tcagcaactt cttgtatgtc accacatatt tccacatcac
    14881 gtgtcttacc tccttttgtg cccgcagtaa cgtgtaaata cggcaccccg tcacggtaag
    14941 ttagtgcggt tcctgttatc atcataagct cccttctcct tagaccagta caacttgtga
    15001 cacgagctaa ttttgcctca gtttttgccg agatgtgact atctcgcttg acattgttac
    15061 gacttctttt tatgtcggat cttcttctgg taggagtttg catgatttcc gttgctctta
    15121 tgccgaataa cttacaaatt gccattttgc gtgtactaat agtgtaagca ctcatccctc
    15181 tatcaatttc gtgctgtaga taatcgttga tatacggctt taaatcatct attgattctg
    15241 caccaggata gtgttctttg caccaacgta catagtgctg acattctatc ttatatgttt
    15301 cataagtctt agtgctatag ataaagttgt agtcaatgcc gttcttttta tcctctttac
    15361 ggctacgccc cataccagct tggagcattg aattgagtct gtcgtatact tgtacagtta
    15421 atcttttagt ttttcgtctt tttcttcgat tactccattg tcccattatt gaacacctcc
    15481 ttgttttttt ttgttctgtt aaatcaatta agcgcagcat agtggctgca agggtcattt
    15541 tttattgttg tccttggcct tggacaccca aactttagtt atttcctatc gctgtattgg
    15601 atcaacgcaa tgtcttatct ttaatcgtta ttgacaagac acagggttct acttgtttcg
    15661 tactcatcat cgttatttaa tctccattct tcttctttcg atatataaat gtcgtatttt
    15721 aattcctgta gcaaaaaaag ataaacagaa atatagtact atctctgttt attcatacat
    15781 gagcaagctc atatacgcat aaacagagaa tttttgcagt tttgaacgtt ttcagacata
    15841 tataagatat aagctaaaaa cttcataagg agggtaaaaa agatgaagag aacagaattt
    15901 agagaggctg aagaaaacct tatttggaaa tgggtacacg aggaattgcc taagcaattt
    15961 ggctttgata cggacgatac tatcgggcat gagactgtga ttcgatttca atgttatgaa
    16021 ggtggaaaaa gttggatttt cgaaaacgat ggtgatgaat tggaactcgg acttggtcgt
    16081 gatgggatgt tcaccgcgac actatcggat attacaaata aaattagtgt agtttcactt
    16141 aatgttctgt gcattggtga cattgaagaa atcaaggagg ttgtataccg tttgtgtgat
    16201 atctttacaa cttcaggcga ttggcattca cttatgtctt ttgtgccaag tatcacagaa
    16261 attgatacgc tgaaaaagtg tattgagtat tacgacgagt tacacagata agtgaataac
    16321 gcataaaatg acataaggat aagagatgtt ttgagcaaac gaaacatctc ttttttattt
    16381 tgttgtgagt aaataaatca tataaattca aataaacatc tgctatacgt ggagacggta
    16441 tgtcttttaa gtagggaaaa agtagatgga tatgtaaatg ttgatttaga tgctaacaaa
    16501 attgttagaa agtctaaaac tggaactgct acttataaag aaattaaaga atatattaat
    16561 gataagtatg gatttacagt tagttcactt aatatagctc aaattaagga caaatgtggc
    16621 cttgagaagc gcaataacta caataaaggt gtagatgggc atataaagta cctacatgtc
    16681 ctgcaaagaa agaaaaagcc cttctagagg cttttaagca ttttggatta atttaatatt
    16741 tagatttccc ggatcttatg gtccgggatt ttttatttgt ttttagattg tttggctaag
    16801 tcttctataa taagttttat gcttgtaaat tgcacaggat ttagtttttt tagattagta
    16861 attgcttctc ttaattcttc gggaagaggg gaatcataat cgaaaaattc catcggagat
    16921 atgtttaaat aactacatat tagaaaaaaa gttcccattg atggaagagc ttttccggat
    16981 tcaatattat taatatagcc tgcatttagt cccagagata gactcaaatc tcgtgcggaa
    17041 acattttttt gatatcttaa ttgagttagt cgttcaataa attgcttttc aaaaattttt
    17101 tcgtccaaaa acagctcctc ctttataaat ggttttgctt attatatttt atcctatcaa
    17161 tgccttgaaa atgatggttt aaatccgcta tatgtattga tgtatcgcat ataaagcgat
    17221 ataatattaa ctagcatagg aataaaagag atattggttt agagagggaa tcagctatga
    17281 aatgaaaatg ctgttttgga tgtggatgat taaactatat ggaaaatgct atataaacaa
    17341 ttatgaaaaa gttttataaa agggagaata agttatgacg aaaaattcaa ttatgagaaa
    17401 gctttttagt attgctatgt gcgcgatact tgtattgtct ttaagtgcat gtggttctaa
    17461 aaaagaaaat gtttcgaata aagatgaaaa taccgcaaat agtgctgctt ccagcgaaaa
    17521 agataatcaa gatataaaga agaatgttgt taaggttgat gacctatctg ttaacccaat
    17581 aacaatcgtt ggctcagacc attccgcaaa gtttggggaa ctggaaggaa cagtaataga
    17641 agctactata aagaataaaa gcaatgattc tgtagatgta tctttggtgg ttgaggcgac
    17701 agtggaatat attgatgact atgaggacaa gcaaacagta aacatgttaa tgtataatcc
    17761 ttctaaaagt tttggtaaca atgcaacttc gtttcattcc ccatacacaa gatacaatgt
    17821 ttatgaagct aataattcag atatacctgt tgatggtctt attccagtgg ggctagcccc
    17881 aaaagaagaa aagacagtac gctactattt tggtgaaaat tatttttacg gatacgattt
    17941 agacaatgat aatgagccaa tggaaattaa aggtggtact attactaaaa tcaaactaaa
    18001 agatgtcaat tgttctgttc ctgattgcga atatgtggca gtagatgaat gggaagactg
    18061 cgttgaatta gtaaatgaga acacacacaa taatgagtat gatagctcta tgttggcata
    18121 cttatctgga gtaattaaaa ataatactga agaacgttgg aaacaagcaa ctgtgcaaat
    18181 ggatgcttat gtagatgatc aagaatacta tgaagttatc aaaagtacag ggagcaccat
    18241 tggaatagac catgattttt ctctgatttt cgatagagcg ttaaggtatc cagagacttc
    18301 agattatgga tcttgttcat atatcgaata tgatagtaac gatgagcaaa aaatagaagt
    18361 cactcctgcg cttatgtact atataccaga aggaaaatag ctggatagta agttggcttt
    18421 tttattaaaa atgcaaataa tatataaaaa tatttgctaa gtttaagaaa aatgttgcta
    18481 ctagattatc aaatttagct taatatcaga ttttctactt cataagaaac tggaaaagta
    18541 tacattcaag agaaaggaaa tggcatctgt gattcttgga atgattgtgg atacagaaat
    18601 cttcaatatc aaaatggtat gagctatgcc tatgcccatt tcaattaaga aagcggaagt
    18661 gctatggatg agcgtatgtt agagtatgga cattcttgta gttctcagga tatctatgaa
    18721 acagtccatc atgatgccgt atatgaaact cagcgtgtga aaactggtac aaaaacagtt
    18781 gttgacaagg cagcttggac agaaacagtt gtgactggtc agaaatgttc ttcttgtgga
    18841 gccacaaaac cagaataacc tggcttaaac aggaaacaga gactcaatct taaatgattg
    18901 ggtctatttt tttgcctatt tttctattac aaaaaaaagt ttccgatttt tagtgcaatc
    18961 gaacgagttt aaaaatgttt ttcttacata ttataatcgg tactgttttt gccgtaccaa
    19021 aaacaggccg ggaaaggata gccggtcgaa aataatttcg gtctttccat tggctaaagc
    19081 ccaatgtgga aatcatccgc cacaaggctc tactgctgat gtgtaagata taaaagaaca
    19141 aaaaacgttc taaaaagaaa ggagtattcc aaattgaaga aaactatctt aaaaagaagt
    19201 gtggcttcga cgctttcttt tctgatggca ttctccacag cagttcctaa ctcagttgtt
    19261 tacgcggaag aatcaggaat tgagagcgag attgctcagg actatgcagc gacacttggc
    19321 cagacacaaa atggaacgac ttcttttgta accgatggga acaagacgaa ggatcctaca
    19381 actcatgtac agggtgacaa ggttgaagtt gctgttgaac cagatgaagg attcaataca
    19441 gctaaaattg aagtcaaaaa cactgacgga acagttgtag cctctcttga agatggtaaa
    19501 gtcactgcaa atgatgaagg taattatact ttcgaaatgc ctgctagtga tgttactgtc
    19561 agtgttagtt ttgaaaccgc aaaaaaaaga agcggctgac gctgaaacag aaacaactag
    19621 aagcgaaact gaaacagaaa aaaatgaaac agaagaagtt aaagaagaag tcaaagctaa
    19681 catatttgac gaattttttg ataaatataa aagctcttgc acttaatatt gaaaagatag
    19741 atggactttg gattatgaac caaggttttt ttttgtgtga aaatagctgt atgtaaaaat
    19801 tagatcttct gaaaatctga catatatata gtgaaggtca ggataaccat gatgagtaag
    19861 ttgtaaggca tcgtgtaaag agtatttcaa aacaaacaga taggagatgg ttatgatgga
    19921 gggataatta ttgaaatgaa ttcagtaaaa atatagcaac cgcagtcatt agaatcgaat
    19981 aagttctaat tggattatgt tgacaagtac tcagcattta ataaatattc ttatcgacct
    20041 tcaagaaaaa actattgata gaaaagaaag aggtaaataa ccatgaaaaa gaaaataata
    20101 ttgaagtaac aagaacagta agatttaagt tgatcggtac atatgatatc ggcgatggat
    20161 tttatgtaga tattgtatta gaccgtgaaa cacaaatgta tgattactgg ttgtataaca
    20221 aagactatga cattaagatc gtttgtatta aattaccaaa gtatcctgat tataaagggt
    20281 ttaaagccga aatgatcaaa aattgccgag atgtggaaag gtacttggat tatattttat
    20341 ctgaaaacta taaaacctat taccgcgata agtatatgca ggattaataa caaagaatag
    20401 atagactcag gttgatttaa agctgggtct ttttatgtgc aaatggtcca aataaatcaa
    20461 aaaacagtaa ttttctttcg gatatccaaa aatcgctgca aaaatatttg acgaatttat
    20521 tgataaaaaa agagactaaa aaaggtttgc tcaatccttt aaattactat ctaagataac
    20581 ttggaatata agttgtagag ccgtatacga gccccgtacg tatggttcaa tgagagggag
    20641 ataactcttg cagttatatt tatacgctgt aaaagtgtaa aatatgatca agatggcggt
    20701 gcatttgaat catatgggca gacttttccc gtaaataaaa acagatacgg tagaggaata
    20761 aataaagact cattttccga tgtcttcata ttacagaata agtatacacc taatgaaaac
    20821 atggctttaa gatacttata aatggtataa aaactacata aaaaaataaa aaatgtaaga
    20881 atgatggtcc tactactaaa gtgacagttt cgtgattcgt tgttattata catatcaacg
    20941 tttaaagtat cgataatttc gtttttgttg cttgtaagta tacaactatc attgttaatt
    21001 aactcataat tttcatcgta attatataag gaatctgtgc tatcataagc gttagaatta
    21061 tcattatgat tttgggtgtt gtttatgtat tctttgtaat atttaaattg attcttatta
    21121 ggtattgatc cgcaagatgt atcgctaagt atttcaccgg tttcatcatt tattatttgg
    21181 aagctagact ttgctttaca tgctttaacg ttactaacgc gaggtctaag accttttggc
    21241 atttttccat agtctctaat aatgtattca gcagcgtcta attgcgattt ttcaagtggt
    21301 ttgcaataac aattgttagg gttggcattg gttaattcaa tccatttttt aaagtctata
    21361 gctccgtttg ataacccatg gatgtggtat tcgtttgttc ctctataaaa ttgcggatat
    21421 aggaaatagt ttacctcttg agttgcaaga aatttatcag caagcttcat aaatgtatta
    21481 atatcagttc taagaggaga atcttctgga gcggtaagtg ttaaaaaatt tgttgcttta
    21541 aaagacatag catggtcttt tgctcttttt tttcgttgtc tatttcgtgc agatattcgg
    21601 tcttcatctg atctgactgt tcctttatta tttggataat attgaaatgg tgtaattttt
    21661 atttcggcat ctccgttttc gtataaagtg tttcttattt ttatcataaa tttcctcttt
    21721 tcacgatgga attccaaaaa aatcatgcag aataggctgt aaaaaaagaa atcctgacat
    21781 attattagtg gatggtgtgt ttggcggcac gcattccaat cgctttataa atttgatcaa
    21841 aagttatatg tctgaaaatt gatttttttg gaggtatatc tgcatgaaaa aaaggtcctc
    21901 ataaatttat gggacctttt ttctgcactt tgaaacgcat tttatatgca tggccattga
    21961 aaagagtttg aaccacttat cgtcttgcct ggcagggaag agtgttaggc attaaccaga
    22021 gaaatcggca gataaaacag aagtgataga caatgatatt tgttaggcat gcttcttgtc
    22081 gttggctgca tcatataaaa acgcttttta cgttaaaaaa gtgctgtgat cacaaggctt
    22141 tttgcaagca aaaatgaaaa ggctatatat aggaagccta cgaaactgga agtgcccatt
    22201 ttgggcctga ttttaaagag tagtgttctg acagagaaca atggaatgac tctctgccgg
    22261 ggattcaagg aaaggaaaaa atgattatgg aaaaaatgat tatggaaaaa atgatgaata
    22321 caaaaataga gggaaacaca tttacaaaga aaatcgggca gacattctat gttgtccgtt
    22381 atcatttcaa tgaagatgca aaggaaacac tgcaggagaa gatcaaccgg atgctggtta
    22441 cagaagcaag ccgaatgaca gtgtattaaa attaggtgga tgtaattgaa gaaaaaatat
    22501 gatatcatgc gagtaacaat tggaatttgg aggattgaat tatgtggaat gaattaggta
    22561 taagcggagg aactgcaatt gttattttga ttgcgctgta tttcgttatc aaatgggcag
    22621 tgaaaaatgg catcaaagaa gcctacagtg ctatcactgg aaagaaaact gatgaagata
    22681 tccaaaacga aaaagtgctg aaagaacttg gattggattc agaagatcaa taacttctaa
    22741 ttgagcgagg tggaaaaatc atggaaaaag ataaaaaaga gaaaatcagg tgtatatgtt
    22801 ttttatttgt ttcaatatgt aactttatag tgtcgatagt gaattttatg gataaaaaca
    22861 ctacacaagc agtaacatat ttattattag gtatttcact tttgctttta tctactacga
    22921 atttgagaaa aaataaataa atacaacttc aagtttgtta aattggaaaa tttgaattta
    22981 atggggagga ggcatgatat gaaaaaaaca agccttgttg ctgtaatact tgtcgggtta
    23041 gctgccgtta tctggacaat cagggctgta cttgaaattg ttttaaaaac ctataatgag
    23101 tcagttttct tgtttgtact gaatgtcctt tgttcggttg tatggattgt gtcatttatc
    23161 gtgaatctga aaaggtatcg ttccaataga gaaaataaat aaataccagt ttgtagaatt
    23221 caaaagttta atatgaaatg tgagatagag cgtatggaaa taatttatac gttctatttt
    23281 tttttgcctt tttgagaaaa agtgattaaa aaactattga cattttgtta ctggagatgc
    23341 gaaaacattt agagaaatgt atgttgcagt taataatgct gaaattcgga attgcaactt
    23401 aaagaaaaga ttacattcac tttgtcaacg aacacttcgt tcacaagtga cagaatatgt
    23461 tcgatacaca aatagaattc tgatattgca agaatacaca ccttctgctg atgaagaaaa
    23521 actatataat tttatctctg attatttgca gaccgataaa ttatacgcac ttccagaggg
    23581 tcagcgtaca cttatcacta tggtccttag aaaattatta gcatcttctt catttgcaat
    23641 ttctggaacg ctaaattcat taatagaaag gttaaataat cttttgtcag atgttgcatt
    23701 gtcacgtggt tattattttt caattaacca tcaaatgata aaaaatcaaa aaggaagaaa
    23761 tattataaat aggattccaa ttgaaaaaat actaattgaa agtgatgcac catttactat
    23821 tggtcttaat tcagattaca atataccttt tgcgaatgaa ctaatagatt ttctttcact
    23881 gagcagaaac attgataaaa ccgttcttta tatgtagata aaaagcaatt ttaaaaattt
    23941 actttcataa tagttattca agtttgatga gcattataat ctctatttaa tactcataaa
    24001 gtattatcaa ataaagcaca ggcctacagg cggaataaag tttcgttagt gggtttgttt
    24061 ttttaaattc aatagtagag ataaatttac gcaaaattcc aagcaataca ttgaatatcg
    24121 catgaatagc gttaaggctt cccctacaaa tgagaaaaat caaattatac catattaaac
    24181 gaccgtttaa tatggtataa taaattgaag gaaattttga ttgtatatcg agttaatttg
    24241 gtaggaaaca acataccctc taaaatggta gcgaggtggt ttaggaatga gtaaaatata
    24301 tggttatgtt cgtatatcga caaaaaagca atccattgaa cgtcaaataa aaaatatctt
    24361 gcaattatat ccgactgcaa atattaaatc tgaaatttat actgggagaa gaaccgaagg
    24421 gcgtgcagtt tggaatttgc tttataaaga aataaagaca ggagatacaa tcgtatttga
    24481 cgaagtatcc agaatgagcc gaaatgctaa agaaggtttc aaattgtata aagaattatt
    24541 tgcaaaaggt gtaaatctag tttttataaa ggagccacat attaatacag atacttatag
    24601 aaatgctatg acaagtacaa ttaacgttga tgtaaatact gcggacgaga atgccgctaa
    24661 gcttttaaac gatatttttg atgctttaaa ttcatatatg tttagattag cagaagagtc
    24721 aattagattg gcatttgatc gttctcaggc ggaagtggat tacttatcac aacgaacaat
    24781 tgaaggattg aatttagcac gagaaaatgg aaaacaaata ggcaatactg caggaagtaa
    24841 aagaacaact caaaaaagta taaaagcaaa agagcttata aagaaacact ccaagaactt
    24901 tggtggttct ctaacggatg gcgaagtgat gcaactagcc aaatgtaccc acaatacata
    24961 ttacaaatat aagcgagaac tattacaaga aatataataa taaaagcaag agattatgcg
    25021 gttaattacg tatcttctct tgcttttttg ttattatttt taaatctcaa aaataggctg
    25081 tatgtaaaaa ttcgagaaac ccaattttct gacacatata taggtgtaag gggtgacaag
    25141 gtctttatct tacgaaataa gatatacaga aagaggtgtt taaactatgg tatttgatat
    25201 ctatgatttt atcacagatt atgtgaaaaa agcaaacaag gttcttgaaa tcgaagaatc
    25261 acagattgtg ttttattctg tggatccaga tgatgatgag ccattggtaa aatattgcaa
    25321 tcatgtgatt tttatcaaaa actgacgatg taattttgga ttatttgttc aaaattgcat
    25381 atgaattgaa tcagcaatcg aatttgtatc agaaaaatac aatgttaacg aataatttta
    25441 attttgccct tgtatattcc aaaatgctga atttatgtca tgaaaaaaat agcttttctt
    25501 acatatacat atcagaacat aacgaggagg tatctgatat ggaaggtaca aaagaaaaaa
    25561 ttgatgtaag aatcgcggag agaccattta tttcaccgaa agaagctgca gcttattttg
    25621 gtattggaat taatacggta agacactttg caaatcagca tcaagaaacc attttatggg
    25681 ttggaaataa aatgatgatc aagcgtagaa aaatggaagc gtatcttgag aatgcatacg
    25741 aattagaaac tccaaaatag attggattga gtaagaaaaa gtaagtgaaa gggcctgtgg
    25801 aatgtgatat agttagtatg tcatatcata ggctctttct tagtggaagg agttataaaa
    25861 tgaagaaaaa agttacaaag gatgggaagc gattaaaaga ttgtgaaaac attcgaaaag
    25921 atggcattta tcagtatcgt tatagagacg cgagtggaaa gtacaaatat ctctactcta
    25981 aaagccttag tgctcttaga attaaggaat ccagaattga aagtgaatta attgctggta
    26041 ttaatccaat ggatgctgac atgactgtga tgcagctgct agagaagtat attagtatca
    26101 aagatggcgt acgatacaac acaaaagttg ggtatcaatt tgtcatgaac gttatggaaa
    26161 aagaaggttt ttccgaacga aaaattagag atattaaagt atccgatgca aaagcttggc
    26221 ttgtaaaact gcaaaatgat ggccgcggat atagttcaat cacctcgatt cgtggtgtaa
    26281 taaaaccagc gtttcagatg gcttttgaag atgatttgat tgtaagaaat ccatttgcat
    26341 ttcaacttaa aggtgttatt cgtaatacca caagagaacg aaaaccatta tcattagaag
    26401 aacaaaaaat gtggttagat tttatgagaa ctgattcgac atattctaag tattatgacg
    26461 atatggttat tttacttgaa acagggatgc gcgtcagtga attttgtggt ctcactgttt
    26521 cagatgttga ttttgagaat aactgtattt acttaaataa acaactgcta cgtactagaa
    26581 atggtgtctt gtttgtggag gaaacaaaaa cttcctgtgg aagaagaaag gttcctatgt
    26641 ctccagcggt ccgtgaagcc ttttcacgtt taattactaa aagaaagaaa cttggtaagg
    26701 gagtgtctgt agatgggttt aaaaacttca ttttatttga taaaaatgcg aagcctaagg
    26761 ttgctttaca cgtagaaaat gagatgagat gggggcttaa gaagtttaaa aagctttatc
    26821 cagaaatgga atacttagaa gtgacaccgc atatactaag acacaccttt gcgactaata
    26881 atatccttta cgggaaaatg ggaattaagg gagccaagtt agttatggga cattctgaag
    26941 cacacgtaac tatggatgtc tatacaggaa gtgactatag tcaggctgca aaagaaattg
    27001 taagtgtttc aaatctgttt gacgaatcat caaaagagtt ttaaaactta taagccataa
    27061 gatgtgtagt actacacatt tttggtgtag tagttaagtg aaaatacgtg gcgaaacgtg
    27121 aagtaaccat aaaagtaaaa taaggagtaa aaagctgaaa agcccttaaa aatggggctt
    27181 ttcaaggctt ttaggaatga aaaaatgagc aaaataaaag tattatttat atgtcatggg
    27241 aacgtatgta gaagtgtaag tgctgagtac attatgaaag atttggcaaa gaaaaatggg
    27301 gtagatctat atgtctctag tgcagctaca agtcgtgaag aaattggtaa tgatatctat
    27361 cctccggcta agcgtaaact aaaagaaaag gggattccat tttctagaca ttatgcaaga
    27421 caagctacaa tgagtgatta tttgtcgtat gactatttga tttgtatgga tgaaaataat
    27481 atacgtaatc taaatcgtat gttcaataat gatccagatc ataaaatcta taaattatta
    27541 tcaagagatg tttcagatcc ttggtacacc aacgattttg aaatggctta taatgatatt
    27601 tttgaaggct gcaaagcttg gttggagaag ttatcatggt gcgccagttc ggtgcagttg
    27661 atttagttag gtgaaactcc taacctggta actcttggag caaagaggcc agtatctagt
    27721 ccttgggcat tgtatc
//
LOCUS       DBMDWL010000005        27543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_667_length_27543_cov_3.016508, whole genome shotgun sequence.
ACCESSION   DBMDWL010000005 DBMDWL010000000
VERSION     DBMDWL010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 27543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 27543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27543
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_27543_cov_3.016508"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="ACZPDN_00650"
     CDS             <1..249
                     /locus_tag="ACZPDN_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024084.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HIR1297168.1"
                     /translation="LCIQELKKRLNTKNFPHEIGVFLGYPLDDVIGFIEHKPYYLVGD
                     WKVYQNVNEAKKQFDVFKQAKEQMLNEIHNGYELSQII"
     gene            complement(396..1427)
                     /locus_tag="ACZPDN_00655"
     CDS             complement(396..1427)
                     /locus_tag="ACZPDN_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1297169.1"
                     /translation="MVKTEEMLKNLEDLINTPSVVGFYPEIHKKLSEMVGNLGYELEF
                     DNKRTAYVRIPGKDSSKTVCVGAHLDTIGLQVRHVMENGWIEVKNLGGVNFHNIEGEN
                     VYIHTRSGKTYEGMVICKSHSTHVFDDARSRERDMFNEAILLDEDVSCAKDVYDLGIE
                     HGDLISVEPRFKRTESGFVKSRHIDDKAMVAAVIEVLRELKEENLTPFYNTLFAFPIF
                     EEIGHGGAYVPSEVSEYVALDIGLIGPDNHGTEKKVSIGASDNYSPYDWELTTKLINL
                     AKENNVHYCVDTYYRFGSDATAAIRAGNNVTPAVFGVGCMNSHGYERTSLQAIEDCVK
                     LTKAYVLSE"
     gene            1471..2181
                     /gene="yaaA"
                     /locus_tag="ACZPDN_00660"
     CDS             1471..2181
                     /gene="yaaA"
                     /locus_tag="ACZPDN_00660"
                     /inference="COORDINATES: protein motif:HMM:NF002543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR1297170.1"
                     /translation="MKIIISPAKKMKIIDDVVIQCTTPLFLLKTTKLLHQLQSLDYEE
                     LKSYLKCSDTIAKTTFEQFQNMSLDKNCTPAILSYSGIQYQYMAPHVFTDDQMKYIQE
                     HLYILSGFYGLLRPLDAITPYRLEMQTKCPFSLYDFWKEDLAKQVDEPILNLASEEYA
                     KIIRKYKPLVDVRFIEENGKEKGVYAKMARGAMVRYLAENEIEDIKDITSFNELDYVF
                     DASQSTEKLYIFQKKLSS"
     gene            complement(2176..3156)
                     /locus_tag="ACZPDN_00665"
     CDS             complement(2176..3156)
                     /locus_tag="ACZPDN_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1297171.1"
                     /translation="MMNVATIGSGVIVDRMIEAMRLDGRYNLYCVYSRTQERAKEFAD
                     KHGIQKYYTDMEEMLNDENVDIVYVASPNSLHYKQSKMALEHKKYVINEKPFTPTLKE
                     CNELFEIAKKNGVYIFEAITNVHLPNYQIIKENLSNCGNIKMVQCNFSQYSSKYQRYK
                     DHIQTNAFDPNFNGGALMDINVYNLHFVTGLFGKPKDVHYFKNVGYNGIDTSGIVIME
                     YPDFIATCTGAKDCSSPYTVYLQGDQGTLIVSGASSGVCKDVFFDAPKKDQIGKKAVD
                     TKEKISIEQPNHMLYECKDFMDIILNKDDKAYTTYKEQTQMVVELLEKLS"
     gene            complement(3153..4568)
                     /locus_tag="ACZPDN_00670"
     CDS             complement(3153..4568)
                     /locus_tag="ACZPDN_00670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1297172.1"
                     /translation="MKGPSFYFQKMSLTRQILTIILTVLMFFIMFFFVFVSDNIDRTI
                     TKQMYELILNRQTPIVGLVDSKQNNSLDDIYAFLASDSVQTNCLIQNDEMNVISNQNS
                     NRKPVNHKVYEYLLEQSKDMDSKTNNVLQGTITVANSKYYYRMIYCHDDIILASFMDD
                     TYATQFRDSLIDSTVYVIVIAFMIVFLIILMWVFSIIHPLNLIRNYIEQIKDGKEVEL
                     HLNRNDEIGEMATAIVKMNDELKKQDKAKEDMIHNISHDLKTPIATIKSYAESIKDGI
                     YPYGTLESSVDVILDNAQRLENKVHSLLYMNRVEYLVASDAEGVVTNMKDVVEQVVLN
                     AAVIRPEIQVITDVEEVFFDGLIEAWRVAIENIMENAFRYAKSYIKIEVREDWLCISN
                     DGPKMPEDRIQSLFRPYEKGEGGRFGLGLSIVSKVCKANHYIVKGENSEDGVRFIIYR
                     KMKKQRKKKENAWVKNKGEKA"
     gene            complement(4630..5289)
                     /locus_tag="ACZPDN_00675"
     CDS             complement(4630..5289)
                     /locus_tag="ACZPDN_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1297173.1"
                     /translation="MKRVINIVEDEKDINNLVAQYLRKEGYEVHSYYTYEEASAHVVD
                     DDVHLWILDIMLDDKSGFDLIEEIRLQGPETPVIFMSARDKEFDRIIGLEKGCDDYIT
                     KPFSPKELVLRVNNIIKRAYRDDNNRISIDGYELDEEQRKIYADNVEIELTTKEFDLL
                     MMFIKNKGIAFSRDKILENVWDENYFGSDRVVDDTLRRLRKKLPNLNIHTIYGYGYRL
                     G"
     gene            complement(5305..6639)
                     /gene="glmM"
                     /locus_tag="ACZPDN_00680"
     CDS             complement(5305..6639)
                     /gene="glmM"
                     /locus_tag="ACZPDN_00680"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1297174.1"
                     /translation="MGKYFGTDGVRGRANEGLTLDMSIKIGQYLGWYYGKEKHAKILI
                     GKDTRLSGDMFELGLAAGATSMGAQVYLLGVCPTPAISYLVRKERFDCGIMVSASHNP
                     YYDNGIKCFNHNGEKMEEELLLEVEKYMDGLTDLDLVTGDHIGEYFEWEDGLEIYMSW
                     LKECVPVDLSGMKILCDLANGSATSTAVETLDSLGATVEAISNSPNGININNKCGSTH
                     PEALQRMMREGDYDIGLAFDGDADRLIMVNSDGELCDGDYILYICSCYMKSVNRLNKN
                     MVVTTVMANLGLYKALDANKIDYIQTAVGDKYVFEEMQKNDYYIGGEQSGHIIFLEHE
                     VTGDGLMTALKILEIMKATGKSLQELSEGLFIYPQLLKNVTVKDKNACLESKELQEVV
                     DSVAQDLGDEGRILVRPSGTEPLIRVMVEAKTDELCAEYVKRVTDFLEANNF"
     gene            complement(6639..7430)
                     /locus_tag="ACZPDN_00685"
     CDS             complement(6639..7430)
                     /locus_tag="ACZPDN_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR1297175.1"
                     /translation="MESPNKKGPSVWFNELRGLLTLIFQKVSKLVDRVLFDKKGSVII
                     SLVLSIMICVVINYEDISLKLFNDTRTTVDLRNVTVEVLADTDQYDVAGVPSTVDVSL
                     TGDATSIQVFRSKGSVQVVADLKKYSEGENIINLKVKNLPEKIEAVVDPATIDVTLSK
                     KITKTFTIQPELLVGTNQKVSDFETPILDTMTVKITASQNQLNSIRIVKALIDCTGQI
                     QDFEANATLAAYDAKGNRVNVSLSPDTVHASVKLAKNTSDKEDSE"
     gene            complement(7414..8352)
                     /gene="cdaA"
                     /locus_tag="ACZPDN_00690"
     CDS             complement(7414..8352)
                     /gene="cdaA"
                     /locus_tag="ACZPDN_00690"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR1297176.1"
                     /translation="MGYTLSDITFLIRLLLDILVVWGLIYSGLRIVRNNSRTIQLVKG
                     VLIIVLVKALAVWLGLNALNYLIEMFLNWGVVVILVVFQPEIRGMLEKVGKTTAILTV
                     DVSVSQIQQMINELVEACSQMSKSKTGALISIERTQSLSDFIRTGIVMDSEVSTELLG
                     TIFQYGTPMHDGAVIIQGNKIACAAAYFPPTTKDLPSKYGARHRAAVGISEITDSITI
                     IVSEETGNISVAVNGQLTQYPPEGLQRYLTNMLVQSSNQNPSSILVPMLNQAKSMSKR
                     AKKQEEDINKDERVKAIEIVNLIEQKKEGSQHGISK"
     gene            complement(8373..9140)
                     /locus_tag="ACZPDN_00695"
     CDS             complement(8373..9140)
                     /locus_tag="ACZPDN_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR1297177.1"
                     /translation="MKLLATDYDGTLRYAQDVMPEDLKSIEEWKNKGNKFVIVTGRSK
                     ESIDAQIKLFNLPCDYLITNNGGMVFDENGNVLLSNYLNFDVCVDILHNIKKLDSVIS
                     YVVNDGIHRHRIVLQSDAVDHRYPNLKPDVTEAELLDMSHFAQIVLSMEDTDSALEVA
                     SKINDLYYEQVVAYPNNFVVDIVPKGISKATGLDFLIAYANINEDDVYTIGDGHNDIP
                     LIEFGAHGSVMSYADEDVKAHAQVEYDSVSDLISKNM"
     gene            complement(9188..10762)
                     /gene="ggt"
                     /locus_tag="ACZPDN_00700"
     CDS             complement(9188..10762)
                     /gene="ggt"
                     /locus_tag="ACZPDN_00700"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR1297178.1"
                     /translation="MEIDYYKNPFASKRMNVIARNGVVCTGNNLATQAGLRMLLQGGN
                     AVDAAIATAACLTVVEPCSNGLGSDGFAIVWMKDKMYGMNSSGHSPYAISADQIDEIP
                     KRGWIPVTVPGAVKGWKALSERFGSLPFKKLLEPAIDYAKNGYPVSAEIARMWKKALN
                     AIPNSEEFKGWYETFTFDGKTPEPGQMICMPKLADALEKIADTNTDALYYGELASRID
                     EESRKFGGFLRKKDLEDHKVEWVDPIHVNYHGYQVWELPPNGQGIVALMALNILNNFQ
                     FKTRDVDCIHTQMEALKMAFADAKEYVTDSKCMEIPVRDLISVDYGKQRAKQIEYRAL
                     PPRKDKPHGSGTVYLCTADKDGNMVSYIQSNYMGFGSGIVVDGVSLQNRGYDFSLDPT
                     HVNYLMPHKKTYHTIIPGFLTKDNKPIGPFGVMGGYMQPQGHMQVVTNMIDFHLNPQM
                     ALDAKRFQWIENKKFILEPRFDENIISELKKRGHEIEIEEELAMFGRGQIIVRLENGV
                     YIAGCESRTDSNIACY"
     gene            complement(11312..11980)
                     /locus_tag="ACZPDN_00705"
     CDS             complement(11312..11980)
                     /locus_tag="ACZPDN_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1297179.1"
                     /translation="MSMIKIAILEYEKETKDIVFQLSKLFMHEDWCFRHYLKASDLAK
                     RMKEEDYQLFVFDEMFKTSRIESVFVHDNPNAIVIYVCENAIKTRGDDERSRVFYISK
                     DNIAVDISAYDQMIRSQCIQSHVYEFNYDGVRMNLAYEDIYYLEKNEKMVYFYTKKGV
                     FHKRDSMNALEEIFKEFGFLRVHVSYIVNSKYIVAWYKDEVELINKVLIPVSRGKKRK
                     ISMK"
     gene            complement(12031..12792)
                     /locus_tag="ACZPDN_00710"
     CDS             complement(12031..12792)
                     /locus_tag="ACZPDN_00710"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1297180.1"
                     /translation="MICKYQELTRLEMEQVDRENTIVLIPLGALEQHGNQAPLGTDTL
                     IAGAMCDYIEKELKDEDINLVLFPVIPVGLSTEHKDFCGSITFKPMTYYHMLYDICES
                     LAHHGFKKLALLVCHGGNTPIANLISREVRSDFKVYPFVLNSGAFDHPDVKATISKGN
                     TFDFHGGEMETSMVMAIDESLVKLETSEKGIPKNTAMKAHLSWLATDWVTEQGKPIGI
                     GGDPRGATKEKGKIILTISAREIVKDLILIRDFKE"
     gene            complement(12795..13412)
                     /locus_tag="ACZPDN_00715"
     CDS             complement(12795..13412)
                     /locus_tag="ACZPDN_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1297181.1"
                     /translation="MKKWNVQTIVLTAMLAGLAGALMSLEFSLPMMPPFYKIDFSDVP
                     SIIALFSLGPVSAALVEVIKLVVKVVTVGTNTAYVGELANLLGIALFILPTWFVYKKM
                     NKTRKAVIVSLVVGILIRTVFACFCNACITLPLYAKAMGMPLDSVVQMVGSVNPSIKD
                     LTSFIALATIPFNLIKISANYIIGYFLMERLAAIRPSFKFIYEAK"
     regulatory      complement(13504..13622)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13681..14619)
                     /locus_tag="ACZPDN_00720"
     CDS             complement(13681..14619)
                     /locus_tag="ACZPDN_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1297182.1"
                     /translation="MIQKKNGIVVVGAIFVDIKGFPQDIYVPDGRNAGYIEYIHGGVA
                     RNVVEDIANLGLNSTFVSIVDNTALGEDVKQRLQNRNVNTDYMKSIENGMGTWLAVFD
                     HNGELAGSISHRPNLMPIYSILEENGDAIIENSDSVVAEIDMDEEIVEKVVYLCQKHN
                     KKLFGVVSNMNIAIERKDLLKQFDCLICNQLESSILFSRDFKEISAEELSHTLIDELQ
                     QSEFNSLIVTLGEAGSLYVKNNGESGFCQARQIEVKDTTGAGDAFCAGTSAGLTYGKS
                     MLEAMKIGSHLASSVIASCENVCPCFLQDKFDIVKK"
     gene            complement(14621..16594)
                     /gene="uvrB"
                     /locus_tag="ACZPDN_00725"
     CDS             complement(14621..16594)
                     /gene="uvrB"
                     /locus_tag="ACZPDN_00725"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR1297183.1"
                     /translation="MKDQFELVSEYKPSGDQPEAIKELVSGLKEDKKFQVLLGATGTG
                     KTFTISNVIAQVNRPTLVFAHNKTLAGQLYSEFKEFFPHNRVEYFVSYFDYYQPEAYL
                     PKTDTYIDKNTKTNEELDMLRMAAVNSVLERRDTIIVASVASIYGASNPEQYRHMIFT
                     LRTGQIIERNELMLALVHQQYKRNDTDPLRGTFRVRGDVIEITPGHTDRYLIRIEMFD
                     DEVERICEVDPVTGHVNQSYQLYIIYPANGYATSMDIINHAADTIQEELDERLAYFDE
                     QGKPLEKERLEQRCRYDIEALREFGVCPGIENYSRHIDGRKLGERPYTLFDYFPDDFL
                     LVVDESHVSLPQIRGMYNGDRARKQVLVDYGFRLPSALDNRPLRFNEFEGLIKNAIFV
                     SATPGDYELDQTHGEIVEQIIRPTGLLDPEVEVRPIEGQIDDLVDEIKERIERHERTL
                     ITTLTVRMAEDLTSYLKNMDLKVAWLHHEVTTIERTEIIHDLRQGKYDVLVGINLLRE
                     GLDIPEVSLIAILDADKEGFLRSRRSLIQIIGRAARNAKGKVIMYADKITESMQEAMD
                     ETARRRSIQMEYNEKHGIVPKTIIKPIHDVVRSKETKEMAAKYLKKKKLPAKQKEKMI
                     KELEQEMKEAARTLDFERAAQLRDILFEMKGEK"
     gene            complement(16642..18057)
                     /locus_tag="ACZPDN_00730"
     CDS             complement(16642..18057)
                     /locus_tag="ACZPDN_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1297184.1"
                     /translation="MSKKSQKLLIILLCVVCFGAGVSVPVVLNKTTSADSKEREKFDT
                     IYSILNSDWYYSNKVKNLDSKLMEQAITGMTTLDKDIHTNYFNLEQAKAFSSSLEGSN
                     VGLGISFYLNENKNFVVSNVYINSTADKKGLKPNDEIISLDDLKCGENDSDTIIKYIK
                     AHEGKSVKTKYLRDGKEYTTNLIPGTFDSTVVCNIYEGYGEIILSSFSENSGKDFSKA
                     LTRLQDAGIKKLVLDLRNNGGGYLNAALEISSSLIPKKSVVFTEKDKNGKVTKQKTKD
                     EFNQVKMDKIVVLQNENTASASEVLIGALKDNLGDKVTTVGTTSYGKGTEQVTVPFSD
                     GTSFKYTIAHWYTPNGTSINKKGFKPDIKVELGEAKTTSYYKFKKKDVIKKDSVDDNA
                     KALQVYLQYLGYNVDRTDTYFSNTSSQGLANFQQDHGLEVTGDCDKTTWDLLVEKVLL
                     KINETPSDDVQRQKAIEEAQK"
     gene            complement(18155..19054)
                     /gene="ftsX"
                     /locus_tag="ACZPDN_00735"
     CDS             complement(18155..19054)
                     /gene="ftsX"
                     /locus_tag="ACZPDN_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR1297185.1"
                     /translation="MKRLRTFLYELKYAFLQMKRHIMLCFSAISAIGITLLLIGAVLI
                     IGLHVNYFSNDVQKDLSIHVILNEDIKDEASIQDINDKISKLKNVDKVEFSSKDNELE
                     LMIKEKGSAFKAYRGDSNPLSNAFFVYLKDASNIRKTSAQIKKIDGVASTAFGGDSVT
                     SLVEMLNMIQKIGLGLVALLVILSLYLIYNTIRTTIDSRQDEIMIMRTVGATNGFISN
                     PFIVEGILIGLLGAVIPYLIVHFGYEKLYTSLGGQLFTPMFAMFKPNTIRFQVGFSII
                     LAGVLIGGFASLLAARKYLKMKR"
     gene            complement(19051..19737)
                     /gene="ftsE"
                     /locus_tag="ACZPDN_00740"
     CDS             complement(19051..19737)
                     /gene="ftsE"
                     /locus_tag="ACZPDN_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1297186.1"
                     /translation="MIDLKDVSKAYPNGVHALNHVNLHIDKGEFVYVIGATGSGKSTL
                     IKVLNGEIVPDEGTVIVGGVNVGALKHRKVPYYRRNVGCVFQDYRLLPKLTVYENIAF
                     ALEVVGMPRKQIRKRVMEVLDLVDLKEKARSYPDELSGGQQQRVTIGRAIANKPMVLI
                     ADEPTGNLDPEKSLEIITLLEKINKKLDTTIMMVTHDKVLVDRFKKRTIMLDKGYVIH
                     DSNRGGYKSL"
     gene            complement(19748..21109)
                     /locus_tag="ACZPDN_00745"
     CDS             complement(19748..21109)
                     /locus_tag="ACZPDN_00745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1297187.1"
                     /translation="MIKYRAGAGFLSLAMACSLIMPVSAEDYSDTETWYAKCSQAQTS
                     QSGVKACQGFQEYQKNRKQNLQDSINSYNSSIASLQSDTSKMEKLAKEQKELVTNLQT
                     QISEKEKSIEMIEKNIEELETKIKAKETEIEAWNEQIETRMKNEQTTIGTNMIIDLIM
                     GSDSLSDMLRRISGVERITEDDQSQIKKLNKLKKELVLQQDEQKRLDADAKSQKAELE
                     NQKKQASELEESYNKLVEEYQKQIAQLEAEKRSAQVDMSSIRDFVISTSFSGTIESVS
                     GFIQPVQGGSKSAGTWAYASGGLHLGLDWAAPVGTSVVAPASGVILYAAAPVGSNSGY
                     LGNWTGYPAGGGNTIQMLCNVNGTLYAVSFFHLSQSIYVSAGQSVSQGQTIALSGNSG
                     NSSGAHTHIEVYNLGSMSVSDAVARFSAGADFAWGTGWSGGSTCEAGSAAPCRERPEK
                     FFS"
     gene            complement(21106..22008)
                     /gene="ftsX"
                     /locus_tag="ACZPDN_00750"
     CDS             complement(21106..22008)
                     /gene="ftsX"
                     /locus_tag="ACZPDN_00750"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR1297188.1"
                     /translation="MLLFFKSLPRLVRNANKDMHRHFSMTFSSIVSIGIALLMALFIF
                     VIYVNVGGFTNQIENEFMIQVSLNPTLEDEEISELSDSIASTSSVKKVTYSSKDDELD
                     ALIKENGPMFKQYKGEDKNPLYDVLNVELKDNTKISKVTKKISKMDGVVKATYGGSAI
                     NTMIQLFKNIRVWGSVFVGLMVLIAVFLIRNTIKMTILVRKDEIAIMRTVGAYNWYIS
                     FPFVLEGIFIGFWGALGPVLICIFGYTGLYHVLNGMFFSNMMTMLKPYPFVLWTSGGI
                     LLIGMVVGMLGSYLAVRKYLRWTR"
     gene            complement(22147..23229)
                     /locus_tag="ACZPDN_00755"
     CDS             complement(22147..23229)
                     /locus_tag="ACZPDN_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR1297189.1"
                     /translation="MKTIEMSNYSVGESCYNEIPSVCEKYNVKKVVLIGGKRALAAAE
                     PRIREAIKETDIIITDSIVYGIECTMTNVHKLTSNPNVQEADVIFAIGGGKAIDTCKT
                     ASIEMDDKMVFSFPTICSNCSAATAIAVVYDDNGALDHYSYPHCPAHIFINPDVIAKA
                     PSEYFWAGIGDALSKQCEVEFATQGKTLDHTATMGLALAKTCTEPLLKYGKQGMEDCK
                     NDTNSAAIEAIALDIVVSTGYVSNLTNQPEYYYNSSIAHAFYNGSCSIARNGHHLHGE
                     VVSFGVLVLYAYAKDEENLMKMAKFNKSIGLPVTLADMDLNESHLEALTQAATKTNEW
                     KNAPFPFTKEEFIAAIKKADAVGQSL"
     gene            complement(23216..24367)
                     /locus_tag="ACZPDN_00760"
     CDS             complement(23216..24367)
                     /locus_tag="ACZPDN_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvI family membrane protein"
                     /protein_id="HIR1297190.1"
                     /translation="MKAQKVGKMAIFSVGAVLFSAHAGGGFATGNQANTYYVGLGWTG
                     IVSAIIAMLLLILTMREAMVMYNQRNLTSAKELFENLYHPFDKLEILFEIFFNIMVLM
                     AVAAAISGAASALQQYFGFNYYACVVAVGIVVLCLTIFGASVVRMASTYMGMAILVTA
                     IVIYGMGIMKGQNIGTILANDFTVNGFMNMPKAILNAFTYAGFQCVTLPTIVACGAIL
                     PDRKSCSKAMWISFVMNAVALVLSVLMLISWKGVYGETTLPTLTSLNAMNANWMVVVY
                     GICLLLCLISTAVTTTFGFVARFENIRVLKNVENPVKKRIIVSAFIIVVSMTISMAGL
                     TNIIKYGYGYCGYLAIAIIIVPFLTVGVYKNYHTENVEVKEGVEVYENN"
     misc_binding    complement(24436..24674)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(24779..25255)
                     /locus_tag="ACZPDN_00765"
     CDS             complement(24779..25255)
                     /locus_tag="ACZPDN_00765"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1297191.1"
                     /translation="MFIQYYDSPLGKMLLAADEIGLTGVWFENQKYYANGLKEPIEEE
                     TAVLKETKEWLNLYFSGKELKALPKMHLIGTQFQKKVWQLLLKIPYGKTMTYKDIARM
                     ISPTMSAQAVGSAVGHNKISILIPCHRVIGSNNNLTGYAGGIKRKEKLLEIEGIHG"
     gene            complement(25310..25385)
                     /locus_tag="ACZPDN_00770"
     tRNA            complement(25310..25385)
                     /locus_tag="ACZPDN_00770"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25350..25352),aa:Ala,seq:tgc)
     gene            complement(25400..25476)
                     /locus_tag="ACZPDN_00775"
     tRNA            complement(25400..25476)
                     /locus_tag="ACZPDN_00775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25440..25442),aa:Ile,seq:gat)
     gene            25623..27263
                     /locus_tag="ACZPDN_00780"
     CDS             25623..27263
                     /locus_tag="ACZPDN_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HIR1297192.1"
                     /translation="MSQIKCPNCGTVFQVDESQYHEILQQVRNEEFEKELKEREKNSS
                     QVIQSKLEKEYESKLSQKALEIKELQANKEQEIISLKEQLKSTEQIIKSETEKAYQEQ
                     LNKKELEIKQLQSDFNQKQNDKEQEVISLKEKLEASTQLTKTETQKEFQEQLNKKDLE
                     ISKLNAKLEQILSQNKINESKTEQEYKLQLQAKENEIQALKSNISNNEKVLKANLENT
                     YQSQLNEKNLEIQSLKQDIDKAKVENELNVKSIKQDLQNQIEQDKMKYQLQEKSLQEK
                     YDTLLQTKDEQIAYYKDFKLKQSTKMIGESLEQHCEIEFNKLRATGFQNAYFEKDNDA
                     HTGSKGDYIYRELDENGVEIISIMFEMKNENDKTATKHKNEDFLKELDKDRNEKNCEY
                     AVLVSMLEPENELYNTGIVDKSHRYPKMYVIRPQFFIPMITILRNAAFNSMQYKNELQ
                     LLRNQNIDITHFEENMNDFKEKFSRNYRIASDKFAKAIDEIDKTILHLQKTKEDLLSS
                     ERQLQLANNKAEDLTIKRLTRNNPTMKQKFDELKKDNK"
     gene            complement(27295..>27543)
                     /locus_tag="ACZPDN_00785"
     CDS             complement(27295..>27543)
                     /locus_tag="ACZPDN_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297193.1"
                     /translation="GNNFLALMSAYSNHILITVLVLALVIYGLITIILKSKEVNAFIK
                     EEKTNENWYARFFKQIPNLLYLIFTILWIIISLFMNMF"
BASE COUNT         8943 a         5692 c         3617 g         9291 t
ORIGIN      
        1 ctgtgtatac aagaactcaa aaaaagactt aacacaaaaa actttcctca tgaaataggt
       61 gtatttctag gatatccatt agatgacgta attggattta ttgaacataa accatactac
      121 ttagttggcg attggaaggt gtatcaaaac gtaaatgaag caaagaaaca atttgatgta
      181 ttcaaacaag cgaaagaaca aatgttaaat gaaattcata atggatatga attatctcaa
      241 attatttaat attttgtttc tatcgaccaa tttcgtgata ttggtctaat aatttaatta
      301 tagatggtat attatatgta tggttataaa tatcatttaa tgtgaaataa taaccaaaag
      361 aaaggatatc gaaaaaaatc gatatccttc ttttattatt cacttaaaac ataagccttg
      421 gttagtttta cacaatcctc aatagcttga agactcgttc tttcatagcc atgtgaattc
      481 atacaaccaa caccaaatac cgcaggcgta acgttgtttc ctgcacgaat agccgctgtg
      541 gcatctgaac caaaacgata atacgtgtcc acgcaataat ggacattatt ttcctttgcc
      601 agattaatta actttgtagt caattcccaa tcataaggag aataattatc actcgctcca
      661 atcgatactt tcttttccgt accatgatta tccggaccaa tcaagccaat atccaacgca
      721 acatattcac ttacttcact aggaacatag gcaccaccat gcccaatctc ctcaaagata
      781 ggaaacgcaa ataatgtatt ataaaaaggt gtcaaatttt cttcttttaa ttcacgcaat
      841 acttcaatca cagctgcaac catagcttta tcatcaatat gacgtgattt aacaaaacca
      901 gattcagtac gtttaaaacg tggttcgaca ctaatcaaat ccccatgctc gatacctaaa
      961 tcatacacat ccttagcaca acttacgtct tcatctagca agatggcttc attaaacata
     1021 tcacgctcac gacttctcgc atcatcaaac acatgagtag aatgtgactt acaaatcacc
     1081 ataccctcat atgttttacc actacgtgta tgaatatata cattttcgcc ttcaatatta
     1141 tgaaaattga ctccacccaa gtttttcact tcgatccatc cattttccat cacatgccga
     1201 acttgcaaac caatcgtatc caaatgtgca cctacacata ctgtcttact tgaatcttta
     1261 cctggtatgc gaacataagc agttcgctta ttatcaaatt caagctcata tcctagattt
     1321 ccaaccattt cacttaattt tttatgaatt tcaggataga aaccaacaac acttggcgta
     1381 ttgatcaaat cctctaaatt ttttaacatt tcttctgttt ttaccataat cttcacttcc
     1441 ttttcttaaa agttattata caatagttgt atgaaaataa ttatatctcc cgccaaaaag
     1501 atgaaaataa tcgatgatgt tgttattcaa tgtacaacac ctttatttct attaaaaaca
     1561 acaaaacttt tacatcaact tcaaagtttg gattatgaag aattaaaatc atatttaaaa
     1621 tgttccgata cgattgccaa aactacattt gaacaatttc aaaatatgag tctagacaaa
     1681 aattgtacac ctgccatact ttcttattcc ggcattcaat atcaatatat ggctcctcat
     1741 gtatttacag atgatcaaat gaaatatatt caagaacatc tttatattct ttccggtttc
     1801 tatggattgt taagaccgtt agatgcgata acgccctatc gtttagaaat gcagacaaaa
     1861 tgcccattct ctttatatga cttttggaaa gaggatcttg cgaaacaagt cgatgaacca
     1921 attttaaatc ttgcaagtga agaatatgcc aaaatcattc gaaaatataa accattagtg
     1981 gatgttcgtt ttatcgaaga aaatggtaaa gaaaaaggag tgtatgccaa gatggctaga
     2041 ggggccatgg ttcgctatct tgctgaaaat gagattgaag acattaaaga tattactagc
     2101 tttaatgaac ttgattatgt ttttgatgcg agtcaaagta cagaaaaatt gtacatattt
     2161 caaaaaaagc tatcgtcatg atagcttttc taataattcg acaaccattt gcgtttgttc
     2221 cttatatgtt gtatatgcct tatcatcttt attcaagatg atatccataa aatctttaca
     2281 ctcatacaac atgtgattcg gctgttcaat actgattttt tcttttgtgt ccactgcctt
     2341 tttaccgatt tgatccttct ttggagcatc aaagaaaaca tctttacaca caccactact
     2401 tgcaccagaa acaatcaatg ttccctgatc cccttgtaga tatacagtat aaggacttga
     2461 acaatctttt gcccctgtac aagttgcgat aaaatctgga tattccataa taacaattcc
     2521 acttgtatca ataccgttat atccaacatt cttaaaataa tgaacatctt tcggtttacc
     2581 aaataatccc gtcacaaaat gaagattata tacattgata tccatcagtg ctccaccatt
     2641 aaagtttgga tcaaaggcat tcgtttgaat atgatcttta tatctttgat attttgatga
     2701 atattgcgag aagttgcatt gtaccatctt aatatttcca caatttgata gattttcctt
     2761 aataatttga taatttggaa gatgcacatt tgtgatggct tcaaaaatat aaacaccatt
     2821 ctttttggca atttcaaata gttcattaca ctcctttaaa gtcggtgtaa aaggcttctc
     2881 attaataaca tacttcttgt gttccaaagc catttttgat tgtttataat gcagtgaatt
     2941 aggacttgcc acatagacaa tatccacatt ttcatcgttc aacatttctt ccatatccgt
     3001 gtaatacttt tgaatgccat gtttatcggc aaattctttc gctctttctt gcgttctaga
     3061 ataaacacaa tataaattgt atcgtccatc taaacgcata gcttcaatca tacgatctac
     3121 aataacacca gatccaattg tcgcaacgtt catcatgctt tttctccttt attttttacc
     3181 caagcgtttt cttttttctt tctttgtttt ttcatctttc gataaatgat aaaacgaaca
     3241 ccatcttcac tattttcacc cttaacaata tagtgattgg ccttacagac tttagaaact
     3301 atggataatc caagtccaaa tcgaccacct tccccttttt cataaggcct aaataaagat
     3361 tgaattctat cttctggcat ttttggacca tcattactaa tacatagcca gtcttctctt
     3421 acttcaatct taatatacga tttcgcataa cggaaggcat tttccataat attctcaatg
     3481 gctactcgcc atgcttcgat caaaccatca aagaaaactt cctcaacatc tgtgatcact
     3541 tgaatttccg gacgaatcac agccgcattc aatacaacct gctccacaac atccttcata
     3601 tttgtgacta caccctcggc atcactagcc actaaatatt caacacgatt catatataac
     3661 aaggaatgta ctttgttttc aaggcgttgt gcattgtcca aaatgacatc aacactactt
     3721 tcaagtgttc cataaggata gattccatct ttaatagatt ctgcatagga tttaattgtc
     3781 gcgattggtg tttttaaatc atgcgaaata ttatgaatca tatcttcttt ggctttatcc
     3841 tgtttcttta attcatcatt catcttcaca atcgcagttg ccatttcacc tatttcatca
     3901 tttcgattta aatgaagctc aacttcttta ccatccttga tctgttcgat ataatttcga
     3961 attaaattca aaggatgaat aatagaaaat acccacatta gaattatcaa aaatacgatc
     4021 ataaacgcaa tcacaataac atacactgtt gaatcaatta atgaatctct aaattgtgtc
     4081 gcatatgtat catccataaa acttgccaaa ataatatcat catggcaata gatcatacga
     4141 taataatatt ttgaatttgc gactgtaatt gttccttgta aaacattatt tgtcttagaa
     4201 tccatatctt tcgattgttc taataaatac tcatacacct tatggttgac cggctttcga
     4261 tttgaattct ggtttgagat cacattcatt tcatcatttt gaattagaca atttgtctga
     4321 acactatcac tcgctaagaa agcataaatg tcatctaaac tattgttttg ttttgaatcc
     4381 actaaaccaa caatcggggt ttgtcgattt agaatcaact catacatttg tttcgtaatg
     4441 gttcgatcaa tattatcact gacgaacaca aagaaaaaca tgataaaaaa catcaaaacc
     4501 gtcaaaataa tcgtaagaat ttgtcgtgtt aatgacattt tttgaaaata aaaagaaggt
     4561 cccttcataa aaatatcacc tcatcacaat cattttacca taagaaaaag aaaaggggat
     4621 acatttgtat caccctaaac gatatccata tccatagatt gtatgaatat ttagatttgg
     4681 aagtttttta cgtaaacgac gcaaagtatc atctacaact cgatcactac cgaaatagtt
     4741 ttcatcccaa acattttcta aaatcttatc acgagagaat gcgattcctt tatttttaat
     4801 aaacatcatc aacaaatcaa attcttttgt cgttaattca atttccacat tatccgcata
     4861 gatctttctt tgttcttcat ccaattcata accatcaata ctaatacgat tattgtcatc
     4921 acgatatgca cgcttgataa tattgttaac tcttaataca agttcctttg gtgaaaatgg
     4981 ttttgtgata tagtcatcac aacctttttc taaaccaata atacgatcaa attctttatc
     5041 acgcgcagac ataaagataa caggcgtttc tggtccttgt agacgaattt cttcaatcaa
     5101 atcaaatcca gatttatcat ccaacataat atccaagatc cacaaatgca catcatcatc
     5161 tacaacatga gcactcgctt cttcatatgt ataatatgaa tgaacttcat atccttcttt
     5221 acgtaaatat tgggcaacta aattattgat gtctttttca tcttcaacaa tgtttatgac
     5281 tcttttcata gggtcaccct ctttctaaaa attatttgct tctaaaaaat ctgtaacacg
     5341 ctttacatat tcagcacaca attcatctgt tttagcttct accatgacac gaatcaatgg
     5401 ttcggtacca cttggacgaa ctaagatacg tccttcatca cctaaatctt gtgctactga
     5461 atccactact tcttgtaact ctttactttc taaacatgca tttttatctt taactgttac
     5521 atttttcaac aattgaggat aaataaataa tccttcactc aattcttgta atgatttacc
     5581 agtagccttc atgatttcta aaatctttaa agccgtcatt aaaccatcac cagtcacttc
     5641 atgttctaag aaaataatgt gtccactttg ttctccacca atatagtaat catttttctg
     5701 catttcttca aatacatact tatcaccaac tgctgtttga atatagtcaa tcttattcgc
     5761 atctaaagcc ttgtataaac ccaagttagc cataactgta gtaacaacca tattcttatt
     5821 taaacgatta acagatttca tgtaacaaga acaaatgtat aggatgtaat ctccatcaca
     5881 caattcgcca tctgagttaa ccatgatcaa acgatcggca tccccatcaa atgccaaacc
     5941 aatatcataa tctccttcac gcatcattct ttgtaaagct tcaggatgtg tggatccaca
     6001 cttattgttg atattgatac catttggtga attgctgatg gcctctacag tagctcctaa
     6061 tgaatctaat gtttcaacag ctgtacttgt ggcagaacca tttgccaagt cacaaagaat
     6121 cttcattcct gataaatcaa caggaacaca ttcttttaac caactcatat agatttctag
     6181 tccatcttcc cattcgaaat attctccaat atgatctcct gtaactaaat ctaagtctgt
     6241 taaaccatcc atatattttt caacttcaag aagtaattct tcttccatct tttcaccatt
     6301 atggttaaaa catttaattc cattatcata atatggattg tgacttgcac tcaccataat
     6361 tccacaatcg aaacgttctt tacgaactaa atatgaaatt gccggagttg gacaaacacc
     6421 aagtaaataa acttgtgcac ccatacttgt agcacctgct gcaagaccta attcaaacat
     6481 gtctccactc agtcttgtat ctttaccaat caagattttc gcatgttttt cttttccata
     6541 ataccaccct aaatattgtc caatcttaat agacatatct aaagttaaac cttcatttgc
     6601 tcttccgcga accccatctg ttccaaaata cttacccatt actctgaatc ctccttatct
     6661 gatgtgtttt tcgctagttt tacactcgca tgtaccgtat ccggtgacaa tgaaacattt
     6721 actcgatttc ctttggcatc ataagctgct aaagtcgcat ttgcctcaaa atcttgaatc
     6781 tgacccgtac agtcaatcaa cgctttgaca atacgaattg aattcaattg attttgactt
     6841 gccgtaatct taactgtcat tgtatctaga ataggcgttt caaaatcact taccttttga
     6901 tttgtaccta ccaacaattc cggttgaatt gtaaatgtct tcgttatttt tttcgaaagt
     6961 gtaacatcaa tcgttgcagg atcaaccacc gcttcaattt tctcaggtaa attttttacc
     7021 tttaaattaa taatattctc accctcagaa tattttttca aatccgcaac aacttgtaca
     7081 ctaccctttg aacggaatac ctgaatactt gtcgcatctc ctgttaaaga aacatccaca
     7141 gtactcggta cacctgctac atcatattga tctgtatctg ccaatacttc tacagttaca
     7201 tttcttagat ccacagtagt tcttgtatca ttaaacaatt ttaaactaat atcttcataa
     7261 ttgatcacaa cacaaatcat aatggataaa accaaagaaa taatcacaga tcctttttta
     7321 tcaaacaata cacgatctac taacttactc accttttgaa agatcagagt caataaacca
     7381 cgcaactcat taaaccatac ggaagggcct tttttatttg gagattccat gttgactgcc
     7441 ctcctttttc tgttcgatta aattcacaat ttcaattgct ttaacacgct catccttatt
     7501 tatatcttct tcctgctttt ttgctcgctt tgacatcgat ttagcttgat tcaacatagg
     7561 aacaagaatc gaagaaggat tttgatttga actctgtact aacatatttg ttaggtagcg
     7621 ctgtaatcct tctggtggat attgcgttaa ttgtccatta accgctactg aaatattacc
     7681 cgtttcttca ctaactataa ttgttattga atctgtaatt tcactgattc ctacagccgc
     7741 tctatgccta gctccatact tacttggtaa atcctttgtg gttggaggaa aataagcagc
     7801 tgcacatgct attttattcc cctgaataat gacagcacca tcatgcattg gcgttccata
     7861 ctgaaatata gttcctaata attcagttga aacctcagaa tccatcacaa tccctgtacg
     7921 aataaaatcg gacaaactct gtgttctttc aatagaaatc aaagctcccg tttttgattt
     7981 tgacatttgt gaacaggctt ccaccaattc attgatcatt tgttgaattt gcgaaacgga
     8041 aacatccact gttaaaatgg ccgttgtttt acccactttt tctaacatgc cacgaatttc
     8101 tggttggaaa acaactaaaa taacaacaac accccagttc aaaaacattt caatcaaata
     8161 gttcaatgcg tttaagccta gccatacagc taaagccttg actaaaacta taatcaatac
     8221 acccttcaca agttgaattg tacgtgaatt attacgaaca attctaagtc cagaatatat
     8281 caatccccat actactaaaa tatccaataa taatcgaatt aggaatgtaa tatcagataa
     8341 ggtataaccc atctaaaata cccccttatt ttttacatat tttttgaaat taaatcactt
     8401 acagaatcat actctacttg cgcatgtgct tttacatctt catctgcata agacattaca
     8461 gacccatgag caccaaactc aatcaatgga atatcattat gcccgtcacc aattgtatat
     8521 acatcatctt cattaatatt ggcatacgca atcaagaaat ctaaacccgt tgcttttgaa
     8581 ataccttttg gtacaatatc aacgacaaaa ttatttgggt atgcaacaac ttgttcataa
     8641 tacaaatcat tgatcttgct agcaacttct aaagctgaat ctgtatcttc catagataag
     8701 acaatttgtg caaaatggct catatctaac aattctgcct ctgtaacatc tggttttaaa
     8761 tttggatagc gatgatccac agcatcagac tgtaaaacaa tacgatggcg gtgaatacca
     8821 tcatttacaa cataactaat tacgctatct aattttttta tgttgtgtaa aatatctacg
     8881 caaacatcaa aatttaaata atttgataat aaaacatttc cattctcatc aaaaaccata
     8941 ccaccattat ttgtgatcaa gtaatcacat ggtaaattaa ataatttaat ttgcgcatca
     9001 attgattctt tagaacgacc cgtaacgatt acgaatttat tccccttatt tttccattct
     9061 tcaatcgatt ttaaatcttc tggcattaca tcctgtgcat aacgcaatgt accatcataa
     9121 tctgtagcta aaagcttcat atttagttcc tcaacttttc tatttactat aattttatca
     9181 cataatttta ataacaagca atattactat ccgtccttga ctcacaacct gcaatataga
     9241 ctccattttc aagtcgaact atgatttgtc cacggccaaa catagctaat tcttcttcaa
     9301 tttcgatttc atgacctcgt ttttttaatt cagaaattat attctcatca aatctaggct
     9361 ctaaaataaa cttcttattt tctatccact gaaatcgttt ggcatccaag gccatctgtg
     9421 gatttaaatg aaaatcaatc atgtttgtga caacttgcat atgaccctga ggttgcatat
     9481 aacctcccat tactccaaat gggcctattg gcttattatc ttttgtcaaa aaaccaggaa
     9541 taattgtatg atatgtcttt ttatgtggca tcaaataatt cacatgagtt ggatctaaac
     9601 taaagtcata cccacgattt tgtaaggaaa ctccatctac gacaatacca ctaccaaatc
     9661 ccatataatt cgactgtata taagaaacca tattaccatc tttatctgcc gtgcataaat
     9721 aaactgtacc tgagccatgt ggtttatctt tacgaggcgg taaagctcta tattcaattt
     9781 gtttggctct ttgtttacca taatcaacac ttatcaaatc acgaacaggt atttccatac
     9841 attttgaatc tgtcacatat tctttggcat ccgcaaaagc catttttaaa gcttccatct
     9901 gtgtatgaat acaatctaca tctctagttt taaattgaaa attatttaaa atattcaaag
     9961 ccattaaagc cacgattccc tgtccgtttg gaggtaattc ccaaacttga tagccatgat
    10021 aattcacatg aattggatcg acccattcca ccttgtgatc ttctaaatct ttcttacgca
    10081 aaaatccacc aaattttctt gattcctcat ctatacgact tgccaattct ccatagtata
    10141 aagcatctgt atttgtatcg gcaatctttt caagtgcatc tgcaagtttt ggcatacaaa
    10201 tcatctgtcc tggttctggt gtttttccat caaaagtaaa agtttcatac cagcccttaa
    10261 actcttcact atttggaatc gcatttaatg cttttttcca catacgagca atctctgcac
    10321 ttaccggata tccatttttt gcataatcaa tagcgggctc taataatttt ttaaatggta
    10381 aagagccaaa tctttcactc aatgctttcc atccttttac agctccagga accgttaccg
    10441 gtatccatcc tcgttttggg atttcatcta tttgatcggc tgaaatcgca taaggagaat
    10501 ggcctgaact attcattcca tacatcttat ctttcatcca cacaatcgca aagccatccg
    10561 atcctaaacc atttgaacaa ggttctacaa cggttaaaca agcagctgtt gcgatagctg
    10621 catctacggc atttccacct tgtaaaagca ttcttaaacc agcttgcgta gccaaattat
    10681 ttcccgtgca aacaactcca tttcgcgcaa tcacattcat tcttttggat gcgaatggat
    10741 ttttataata atcaatttcc atatctttct caccgagacc attatacaaa aattagagca
    10801 gacattccat ctgctctgat ttatttattc acttttttta accggataac ttttctacct
    10861 tatccaattt ttgtatcatc cacattatga ttttattcta ttcattttcc ttaagcattt
    10921 tctcaagtat gaccatatat gagtatcttt aagttccatg acttagattg gatgatattc
    10981 ttaacatttc aagtactcta tcctttttgt gattattcac tcgataaaaa ccctttatct
    11041 aaatcctatt tccttttcct gttaagttcc attgaatgtt ttttctagaa ttcttctagc
    11101 ttaacttttg gagccttctc tccatagctt caacttttta tagttaaaac attccgtttc
    11161 aactctatgt tattgtcatc ttctcaccgc tctctttttg tcttaagaga agttttctct
    11221 gctcttactt ctctttacac tttcattata tacaagaaaa acaaaaagaa aaggcaagtg
    11281 gtaaaactcg ccttttaact ggtaaaaccg gttacttcat cgatattttt cgcttttttc
    11341 ctctcgaaac tggaattaaa actttgttta ttaattcaac ttcatctttg taccaagcca
    11401 caatatattt ggaattcaca atatacgata catgtacacg taaaaagcca aactctttaa
    11461 aaatttcttc aagtgcattc atactatcac gcttatgaaa aacgcctttt ttcgtataga
    11521 aatacaccat cttttcattc ttttccaaat aataaatatc ttcatacgca agattcatac
    11581 gaaccccatc ataattaaat tcatatacat gactttgaat acactgtgaa cgaatcattt
    11641 gatcataagc cgaaatatca actgcgatat tatcctttga tatataaaac acacgacttc
    11701 tttcatcatc accacgcgtt ttaatggcat tttcgcacac ataaatcaca atcgcattgg
    11761 gattatcatg tacaaataca ctttcaattc gactcgtctt aaacatttca tcaaacacaa
    11821 acaattgata gtcttcttcc ttcattcgtt tcgcaagatc actcgccttt aagtaatgac
    11881 gaaaacacca atcctcatgc ataaacaatt tagataattg aaaaacgatg tccttagttt
    11941 ctttttcata ttctaaaatg gcaattttta tcatactcat tattataaag attgaaaaca
    12001 ttttaagcaa atgtaaacta tttcaaaatg ttattcttta aaatcacgaa ttaaaatcaa
    12061 atccttaaca atttcacgag cactaatcgt taatataatt tttccctttt ccttagtggc
    12121 ccctctagga tctccaccaa tcccaatggg ctttccttgt tcagttaccc aatctgtagc
    12181 tagccaagat aaatgtgctt tcatggccgt atttttagga ataccctttt cacttgtttc
    12241 taatttaact aaactttcat caatcgccat gaccatagaa gtttccattt ctccaccatg
    12301 aaaatcaaat gtattccctt tagaaatcgt tgcctttaca tctggatgat caaaggctcc
    12361 tgagttcaat acaaatggat acactttaaa atcacttctt acttctctag aaattaaatt
    12421 ggcaatcggt gtattgcctc catgacatac aagcaaagcc aactttttaa atccatgatg
    12481 cgctagactt tcacatatgt catacaacat atgatagtaa gtcataggtt taaaagtaat
    12541 ggatccacaa aagtctttat gttctgtact caatccgaca ggaataactg gaaacaatac
    12601 taaatttatg tcctcgtcct ttaactcttt ttcaatatag tcacacattg caccggcaat
    12661 gagtgtatcc gtaccaagtg gtgcttgatt tccatgttgt tctaatgcgc ctaacggaat
    12721 caaaacaatt gtgttttctc gatcaacttg ttccatttct aaacgcgtta attcttgata
    12781 cttacaaatc atacttattt tgcctcgtag ataaatttaa agcttggacg aatggctgcc
    12841 aatctttcca tcaaaaaata tccaatgatg tagttagctg agattttgat caagttaaat
    12901 ggaattgtcg caagtgcaat aaatgaagtt aaatctttaa tacttggatt tacagaacct
    12961 accatttgta caacagaatc aagtggcata cccatggcct ttgcatataa tggcaaagta
    13021 atacatgcat tacagaaaca tgcgaatacg gtacgaatta gaattccaac tactaaagaa
    13081 acaataacag cttttcttgt tttattcatc ttcttgtaca caaaccatgt tggtaaaata
    13141 aatagggcaa tacctaaaag atttgcgagt tcacctacat aagctgtatt tgttcctact
    13201 gtcacaactt ttacaactaa tttaatgact tcaaccaaag cagctgacac aggtcctaaa
    13261 ctaaataggg caataatact tggtacatca ctaaaatcaa tcttgtagaa cggtggcatc
    13321 attggtaaag aaaattccaa tgacatcaaa gctcctgcaa gacctgcaag catcgccgtt
    13381 aatacaattg tttgaacatt ccattttttc atctttttac ctccttttga ttcggataag
    13441 ctcttccaaa aggaaaaccc caagaaaaat cttctcgggg tctaaaaaat acaaaaaata
    13501 cttttgtgtc ttctaccatc cagactatac tgtcggtctt ggaattacac caagtcagcc
    13561 acttgcgtgg gtcgcggact tttaccgccg gtagggaatt gcaccctgcc ccgaagaact
    13621 cattgaatct atgttcgtga ataaaatagc acataatctt tcatcatgca actaatttgt
    13681 tcattttttc acaatatcaa atttatcttg caaaaaacaa ggacatacat tttcacacga
    13741 agctatgaca gaactagcta aatgactacc tatcttcata gcctcaagca tactcttacc
    13801 ataagttagt ccagcacttg tcccagcaca aaatgcatct cctgctcctg ttgtatcctt
    13861 tacctcgatt tgacgagcct gacaaaagcc gctttcacca ttgtttttca catataaaga
    13921 tcccgcttca ccaagtgtta caatcaaaga gttaaattcg gattgctgca gttcatcaat
    13981 caatgtatga cttaattctt ctgctgatat ctctttaaaa tctcttgaaa aaagaatact
    14041 cgattctaat tgattacaga tcaaacaatc aaattgcttc aaaagatctt ttctttcaat
    14101 cgcaatattc atattgctga caacaccaaa taatttttta ttatgttttt gacataaata
    14161 aacaactttt tcaacaattt cctcgtccat atctatttca gccaccacag aatcgctatt
    14221 ttcaataatt gcgtctccat tttcttctaa aattgaatag atcggcatta aattcggacg
    14281 atgagatatt gaacccgcta attctccatt atgatcaaaa acagctagcc atgtacccat
    14341 accgttttcg atagatttca tataatctgt attcacattt cgattttgca atctctgttt
    14401 aacatcttct cctaatgccg tattatcaac aatactaaca aatgttgagt ttaaacctaa
    14461 attcgcaata tcttctacaa catttcgtgc tacaccacca tgaatatact caatatatcc
    14521 tgcatttctc ccatcaggaa catatatatc ttgtggaaaa cctttaatat ccacaaaaat
    14581 cgcacctact acgacaattc cattcttttt ttgaatcatg ttatttttca cctttcatct
    14641 caaataagat atctcttaat tgtgctgctc tttcgaaatc taaagtacga gctgcctctt
    14701 tcatttcctg ctccaattct ttgatcatct tttccttttg ttttgccggc aactttttct
    14761 tcttcaaata cttagctgcc atttcctttg tttctttaga acgaacgaca tcatggattg
    14821 gtttgataat tgtctttgga acaattccat gtttttcatt atattccatt tgaatcgatc
    14881 tacgtcttgc ggtttcatcc atggcttcct gcatactttc tgttatctta tctgcataca
    14941 taataacttt acctttggca ttacgtgctg cacgaccaat aatctgaatt aaggatcttc
    15001 gtgatcttaa aaagccttct ttatctgcat ctaatattgc gatcaagcta acttccggaa
    15061 tatccaaacc ttctcttaat aagttgatac ctacaagtac atcatattta ccttggcgta
    15121 aatcatgaat gatttcagta cgttcaatcg ttgtaacttc atggtgtagc catgctacct
    15181 ttaaatccat attctttaaa taggaagtta aatcctctgc catacgaacc gtcaatgtcg
    15241 tgatcaatgt tctttcatgt ctttcaatac gctccttgat ttcatctact aaatcatcaa
    15301 tctgcccttc aataggacgc acttcgactt caggatctaa taatcctgtt ggacgaatga
    15361 tttgttcaac aatttctcca tgcgtttgat ccaattcgta atcacccggt gttgcagata
    15421 caaagatggc atttttaatc aatccttcaa attcattaaa acgcaaagga cgattatcca
    15481 aagcacttgg aagtctaaat ccataatcca ctaatacttg ttttcttgca cgatctccat
    15541 tatacatacc tcgaatttgt ggtaaagaaa catggctttc atctactaca agtaaaaaat
    15601 cgtctgggaa ataatcaaat agtgtataag gtctttcacc tagttttcta ccatcaatat
    15661 gccttgaata gttttcaatg cctggacata caccaaactc ccttaaggcc tctatatcat
    15721 agcgacatcg ttgttctaaa cgttcttttt ctaatggttt accctgctca tcaaagtagg
    15781 ctaatctttc atccaattct tcttgaatgg tatccgccgc atgattgatg atatccatac
    15841 ttgtcgcata gccgttagct ggatatataa tatataactg ataagactga ttgacatgtc
    15901 ctgtcactgg atccacttca cagattcttt ccacttcatc atcaaacatt tcaattcgaa
    15961 tcaaataacg atctgtatgt ccaggtgtga tttcaatgac atcgccacgc actctaaaag
    16021 ttcctcttaa tggatctgta tcattacgtt tatattgttg atggaccaat gccaacatca
    16081 attcatttct ttctatgatt tgtccagttc gcaaagtaaa aatcatatga cgatattgtt
    16141 ctggattgct tgcaccataa atagaagcaa cacttgctac aataatcgta tctcttcttt
    16201 ctaagacact atttacggct gccattctaa gcatatccaa ttcttcattg gtctttgtat
    16261 ttttatcaat atacgtatcc gtttttggca aataagcttc cggctgataa taatcaaaat
    16321 aagatacaaa atattccacg cgattgtgtg gaaagaattc tttaaattcc gaatataatt
    16381 gtcctgccaa agttttatta tgtgcaaaca ccaaggttgg tcgattgact tgagcaatca
    16441 catttgagat ggtaaatgtt tttcccgttc ctgtagctcc taacaatact tgaaactttt
    16501 tatcttcttt taaaccagaa accaattctt taatcgcttc tggctgatct ccactcggtt
    16561 tatattcaga cactaattca aactgatctt tcatagcttc acatccttct tctccattat
    16621 atacaaaaag taggcttata cctacttttg agcctcttca atcgcttttt gtctttgtac
    16681 atcatcgcta ggtgtttcat ttatcttcaa caatactttt tccaccaaaa gatcccacgt
    16741 tgttttgtca caatcccctg ttacttctaa gccatgatcc tgttgaaaat ttgccaaacc
    16801 ttgagaactc gtattcgaaa aatatgtatc tgttcgatca acattatatc ctaaatactg
    16861 tagatacact tgaagagctt tggcattatc atccacacta tcttttttaa taacatcctt
    16921 tttcttgaat ttgtaatagg atgttgtctt agcttcacca agctcgactt taatatctgg
    16981 tttaaatcct tttttattaa tacttgttcc atttggcgta taccaatgtg ctattgtata
    17041 tttaaaactt gttccatccg agaatggtac ggtcacttgt tctgtacctt taccatagga
    17101 tgttgtacca actgtagtta ctttatcccc aagattatct tttaaagccc caatcaacac
    17161 ttccgatgca cttgccgtat tttcattttg taaaacaaca attttatcca tctttacctg
    17221 attgaattca tcttttgtct tttgtttggt cacttttcca ttcttatctt tttctgtaaa
    17281 tacaactgac ttttttggaa tcaaagaact tgaaatttct aatgcagcat ttaaatagcc
    17341 accaccatta ttacgtagat ccaatactaa ttttttgatg cctgcatctt gtagacgagt
    17401 caatgctttt gagaaatctt ttccactatt ttctgagaaa ctcgataaaa taatttctcc
    17461 atatccttca tatatattgc atacaactgt tgaatcaaaa gtaccaggaa ttaaatttgt
    17521 agtatattct tttccatcac gaagatattt tgtcttcacg ctcttacctt catgcgcctt
    17581 aatatattta atgattgtat ccgaatcatt ttcaccacat tttaaatcat ctaatgaaat
    17641 aatttcatca ttgggtttta agcccttttt atctgccgtt gaattaatat atacattgga
    17701 tactacaaaa tttttattct catttaaata aaaggaaatt cccaatccaa cattgcttcc
    17761 ttcaagacta cttgagaagg ctttggcctg ttccaaatta aaatagtttg tatgaatatc
    17821 cttatccaat gtagtcattc ctgtaatggc ttgttccatt aatttggaat ctaaattttt
    17881 cactttattt gaataatacc aatccgaatt caaaatagaa tagattgtat caaatttttc
    17941 gcgttcttta ctatcggctg atgttgtttt atttaaaaca acaggtacac taacaccagc
    18001 accaaaacat acgacacata ataatatgat taataatttc tgactctttt tactcataaa
    18061 ctcatcctac tctaaaacaa aatgggacga atcattcgcc ccaataattc tatacaaaaa
    18121 gtatacgaaa caatttttta ttctgcaatg gtttttatcg tttcattttt aaatattttc
    18181 ttgccgcaag caaagaggca aagccaccaa tcaaaacacc cgcaagaatg atagagaatc
    18241 ctacttgaaa acggattgtg tttggtttaa acatggcaaa catcggtgta aataattgtc
    18301 cacctagact tgtatatagc ttttcatatc caaaatgaac aattaaatac ggaattacag
    18361 ctccaagtaa tccaattaag attccttcta caataaacgg attactaata aagccatttg
    18421 tagcacctac agttcgcatg atcatgattt catcttgcct tgaatcaatt gttgtgcgaa
    18481 tcgtattata gatcaaatat aaggataata tgaccaacaa tgcaacaagg cccaaaccaa
    18541 ttttttgaat catattcaac atttcaacta aagaagtgac actatctcca ccaaaagctg
    18601 tactcgctac gccatcgatt ttcttgattt gtgcacttgt tttacgaatg ttagaagcat
    18661 cctttagata aacaaagaac gcatttgaca aaggattcga atctcctcta tatgctttaa
    18721 aagccgaacc tttttctttg atcatcaatt ccaattcatt gtctttggat gaaaattcaa
    18781 ctttatccac attttttaat ttggagattt tatcatttat atcttgaata gaagcttcat
    18841 cctttatatc ctcatttaat atgacatgaa tgcttaaatc tttttgtacg tcattcgaaa
    18901 aataattcac atgaagtcct ataattagaa cagctccaat taataataac gtaataccaa
    18961 ttgcgctaat ggcggaaaag cataacatga tatgtctttt catttgtaaa aaggcatatt
    19021 taagttcata taaaaaagtt cttaaccttt tcatagactc ttatacccac ctctattcga
    19081 atcgtgaatc acatatcctt tatcgagcat aatcgtacgt tttttaaaac gatccaccaa
    19141 aactttatca tgtgtaacca tcataatcgt cgtatcaagt tttttattga ttttttcaag
    19201 caaggtaatg atttctaaac ttttttctgg atctagattt cctgttggtt catccgcaat
    19261 caaaaccatt ggcttatttg cgatggcacg cccaattgtg acacgctgtt gttgtccacc
    19321 agataattca tctggatagc ttctggcttt ttctttcaag tctacaagat ctaagacttc
    19381 catgacacgc tttcgaattt gtttacgtgg catacccaca acttctagag caaacgcaat
    19441 attttcataa acagttagct taggtaataa acgataatct tgaaaaacac acccaacatt
    19501 acgtctgtag tatggaactt ttctatgttt caatgcacct acattgactc cacctacaat
    19561 aacagttcct tcatctggaa caatttcgcc atttaacact ttgatcaatg ttgactttcc
    19621 tgaaccagta gctccaatca catacacaaa ttcaccttta tcaatatgta aatttacatg
    19681 atttaaagca tgtaccccat tgggatatgc ttttgaaaca tcttttaaat caatcataat
    19741 gttcctccta actaaagaac ttttctggtc tttctcgaca tggtgctgca cttcctgctt
    19801 cacaagtact tcctccagac catcctgttc cccacgcaaa atcagctccg gcactaaagc
    19861 gagctaccgc atcactaaca gacatcgatc ctagattata aacttcaata tgtgtatgtg
    19921 ctccacttga atttcctgaa tttccagata atgcgattgt ttgtccttgt gaaacactct
    19981 gtcctgcact tacataaatg ctttgcgata aatggaagaa acttacggca tataatgtgc
    20041 cattcacatt acataacatt tgaattgtat ttcctccacc ggcaggataa cctgtccagt
    20101 tacctaaata cccactatta cttcctactg gagctgcggc atataaaata acaccacttg
    20161 ctggagctac aacacttgtt cctacaggag ctgcccaatc tagacctaaa tgaagtccac
    20221 cactagcgta tgcccatgta ccagcacttt tacttcctcc ttgtacgggt tgtataaagc
    20281 ctgaaaccga ttcaatggta ccagagaagc ttgttgaaat cacaaaatca cgaatcgaac
    20341 tcatatctac ttgtgcagat cttttttccg cttctaactg agcgatttgt ttctgatatt
    20401 cttcaactag cttgttataa gattcctcta attcacttgc ttgtttcttt tgattttcta
    20461 attctgcctt ttgacttttt gcgtctgcat ctaaacgctt ttgttcatct tgctgcaaaa
    20521 caagttcttt ctttaattta tttaactttt taatttgcga ctgatcatct tcagtaattc
    20581 tttcaacacc tgaaatacga cgaagcatat cactcaaact gtccgatccc ataatcaaat
    20641 caataatcat atttgtacca atcgttgttt gttcattttt cattcgcgtt tcaatttgtt
    20701 cattccaagc ttcgatttca gtttccttgg ccttgatttt cgtttcaagc tcttcaatat
    20761 tcttttcaat catctcaatc gatttttctt tttcactaat ttgtgtttgt agatttgtaa
    20821 ccaattcttt ttgttcctta gcaagctttt ccatttttga agtatcactc tgcaaagaag
    20881 ctatgcttga attataagaa ttaatactat cttgaagatt ttgtttacga ttcttttgat
    20941 attcctgaaa tccttgacaa gcttttactc ccgattgaga agtttgcgct tggctacact
    21001 tagcatacca agtttctgta tccgaatagt cttcggctga aacaggcata atcaatgagc
    21061 aggccatagc taagcttaaa aaacctgcac cagctctata ttttatcatc gtgtccacct
    21121 caaatatttt ctaactgcca aataacttcc tagcattcct acaaccattc caatcaaaag
    21181 aatgccacca gaagtccaaa gtacgaatgg atatggtttt aacattgtca tcatattgct
    21241 aaaaaacata ccattcaaca catggtataa acctgtatat ccaaatatac aaatcaaaac
    21301 agggcctaaa gcaccccaaa atccaataaa aattccttct aatacaaatg gaaaagagat
    21361 gtaccaatta taggcaccaa ctgttcgcat gatcgcaatc tcatctttac gtactaaaat
    21421 cgtcatttta atcgtatttc gaatcaagaa tacggcaatc aacaccataa gtcctacaaa
    21481 gacacttccc catacacgga tattcttaaa caattgaatc atcgtattga ttgcactacc
    21541 tccataggta gccttaacaa caccatccat cttagaaatt ttttttgtca cctttgaaat
    21601 tttcgtatta tcttttaatt ccacatttaa aacatcatat aaaggatttt tatcctctcc
    21661 cttatattgt ttaaacatag gaccgttttc tttgatcaac gcatccagtt catcatcttt
    21721 acttgaatat gtgactttct ttacagaaga cgtagaagca atcgaatccg ataattcact
    21781 aatttcttca tcttctagtg ttggatttaa agaaacttga atcataaatt cattttcaat
    21841 ttgattggta aatcctccga cattcacgta aatcacaaat ataaacaagg ccatcagtaa
    21901 agcaatgcca atcgatacaa tggaagaaaa agtcattgag aaatgacgat gcatatcttt
    21961 attggcatta cgcactagtc ttggtaaact cttaaaaaac agtaacattt tatcacctac
    22021 attactaatt ataacacagc tatcaaattg acctacaaat cacaaaattt cacatacacc
    22081 ttaattctaa tttttccatg tgaaaataac attaaaaccg aaaaaaaaga tcgatttctc
    22141 gatctcttat aaactttgac ctaccgcatc tgctttttta atagccgcaa taaactcttc
    22201 ttttgtgaat gggaaaggtg catttttcca ctcatttgtt tttgtagctg cttgggtcaa
    22261 agcttctaaa tggctttcat tcaaatccat atcagccaaa gtcactggta agccgattga
    22321 tttattgaat tttgccattt tcattaagtt ttcttcgtct ttggcatatg catacaatac
    22381 taatacacca aaagatacta cttcaccatg taaatggtgt ccgttacgtg caattgagca
    22441 gcttccatta tagaaggcat gtgcaatgct tgaattataa taatattctg gttggtttgt
    22501 taagtttgaa acgtatccag tggatacaac aatatccaag gcgatggctt cgattgcagc
    22561 cgaatttgta tcatttttac aatcttccat accttgttta ccatatttca acaatggttc
    22621 tgtacatgtt ttagccaatg ccaaacccat tgtagctgta tgatctaaag tctttccttg
    22681 cgttgcgaat tcaacttcac attgtttaga caaggcatcc ccaattcctg cccagaaata
    22741 ttcgcttggt gcttttgcga taacatctgg attgataaag atatgtgctg gacaatgcgg
    22801 atatgaatag tggtccaacg ctccattatc atcatagact acggcaatag ctgtagcggc
    22861 cgaacagttt gaacagattg ttggaaatga gaagaccatt ttatcatcca tttcaatgga
    22921 tgcagtttta caagtatcga tagcttttcc tccaccaatc gcaaagataa catcggcttc
    22981 ttgtacgttt ggatttgaag tcaatttatg tacgtttgtc attgtacatt caataccata
    23041 tacaattgaa tctgtaataa taatatcagt ctctttaatg gcttcacgaa ttcttggttc
    23101 agcagctgcc aaagctcttt taccaccaat caatacaacc tttttaacat tgtatttttc
    23161 acatacactt gggatttcgt tgtaacaact ttctccgact gaataattag acatttcaat
    23221 tgttttcata aacttctact ccttctttta cttcaacatt ttctgtatga taattcttgt
    23281 atacaccgac tgttaagaat ggaacgataa tgattgcgat agctaaatat ccacaataac
    23341 catatccata tttaataata tttgttaatc cagccattga aatcgtcata gatactacaa
    23401 taataaatgc agatacaatg attcttttct ttactggatt ttctacattc tttaaaacac
    23461 gaatattttc aaagcgagcc acaaatccga atgttgttgt tactgctgta gaaattaagc
    23521 aaagtaataa acaaatacca tatacaacaa ccatccagtt ggcattcatt gcatttaaac
    23581 ttgtcaatgt aggtagagtt gtttctccat aaacaccctt ccaagaaatt aacatcaata
    23641 cacttaatac taatgcgaca gcattcatca caaatgagat ccacattgct tttgaacaag
    23701 atttacgatc tggtaagata gctccacaag caacgatcgt tggtaatgtc acacattgaa
    23761 agcctgcata tgtaaatgca tttaagatag ctttaggcat attcataaag ccatttacag
    23821 taaagtcatt tgctaaaata gttccaatat tttgaccttt cataattccc ataccataaa
    23881 taactatggc tgttactaag atagccatac ccatatatgt acttgccatt cttacaacac
    23941 ttgcgccaaa aatggttaag caaagaacaa cgattccaac cgcaacgaca caagcataat
    24001 aattaaatcc aaagtattgt tgcaatgcac ttgcggctcc actaatagct gctgcaacag
    24061 ccatcaaaac catgatatta aagaagatct caaataagat ttccaactta tcaaatggat
    24121 gatataagtt ttcaaacaat tcctttgcgc ttgtcaaatt tctctggtta tacatcacca
    24181 tggcttctcg cattgtcaat atcaatagca acatagctat tatggcactc acaataccag
    24241 tccatcctaa acctacataa tatgtatttg cctgatttcc tgtggcaaat ccacctccag
    24301 catgtgctga gaataatacg gcacctactg aaaatatcgc catttttcca actttttgtg
    24361 ctttcatact tttcctccta tagcgtgcac aacaagctaa caaaaaaacc aatcgtttct
    24421 tgctgcctgt tagacagaag agacgaaagg ttagaattac aatacttccg cggtaccact
    24481 cttcttcatt catttaagaa tgcactcatt tcagatgcta tcacatcctg cttcattaac
    24541 gttaagccta cgttcaagtc ttatcaacct gaccactcca tggtaagttc aaagttccat
    24601 ctcttatgtc ttgcaccaac cgacaactct ctaacaaaga ctttcctttt actactccat
    24661 ttccttgtgt tttgtgcgtt tataataaca ctacattttc atttatgcaa gcattttctt
    24721 acatttgttt tcagtaaatt caaacctacg ttttacattc atcaaaaaaa ggtacgcatt
    24781 acccatgtat accttcaatt tctaataatt tttctttcct cttaattcca cctgcatatc
    24841 cagtcaaatt attattggat ccaatcaccc tgtgacaagg aatcaaaatt gatatcttat
    24901 tatgtcctac agcacttccc accgcttgtg ctgacattgt tggtgaaatc attcttgcga
    24961 tgtccttata tgtcatagtt ttaccatagg gtattttaag aagaagttgc caaactttct
    25021 tttgaaactg tgttccaatc aaatgcatct taggtaaagc cttgagttct ttaccactaa
    25081 aataaagatt taaccattct tttgtttctt ttaatacagc cgtttcttct tcaattggtt
    25141 cttttaaacc attcgcataa tatttttgat tttcaaacca aacaccagtc aatccaattt
    25201 catcggcagc caataacatt ttccctaatg gtgaatcata atattgtata aacataatcg
    25261 tcctctatat agaaaaaaag ccatccgaag atgacttact ttttaaaatt ggtggagcgt
    25321 atcgggatcg aaccgatgac cccctgcgtg caaggcaggt gctctcccag ctgagctaac
    25381 accccatatc ttcagcaaat ggtgggcctg aatggacttg aaccaacgac ctcaccctta
    25441 tcaggggtgc gctctaacca cctgagctac aggcccattc gctgaatgcc tattaaatat
    25501 accatgtgat tttatattat gcaagtcttt ttttgatttt ttttaaatat tataattaat
    25561 ctagttttag acataaatat tgtcaattta atgattataa tcatataaaa cggaggtgcc
    25621 ttatgagtca aataaaatgt cctaattgtg gaactgtatt tcaagttgat gagagtcaat
    25681 atcatgaaat cttacaacaa gtccgtaatg aagaatttga aaaggaatta aaagaaagag
    25741 aaaagaattc aagccaggtt attcaatcta aattagaaaa agaatatgaa tctaaattaa
    25801 gccaaaaagc tttagaaatc aaagagcttc aggcaaataa agaacaagaa atcatttctt
    25861 taaaagaaca attaaaatct actgaacaaa ttatcaaaag tgaaactgaa aaggcttatc
    25921 aagaacaatt aaataagaaa gagcttgaaa tcaaacagct acaatcggat tttaatcaaa
    25981 aacaaaatga taaagaacaa gaagttattt cattaaaaga aaaactagaa gcgagtacac
    26041 aattaacaaa aactgaaaca caaaaagaat ttcaagagca gttaaacaaa aaagatttag
    26101 aaatatctaa actcaatgcc aaattagaac agattctttc acaaaataaa atcaatgaat
    26161 ctaagactga acaagaatat aaattacagc tgcaagcaaa ggaaaatgaa attcaagctt
    26221 taaaaagtaa catttcaaat aatgaaaagg tattaaaggc aaatctagaa aatacttacc
    26281 aatctcagtt aaatgaaaag aaccttgaaa ttcagtcttt aaaacaagat atcgataaag
    26341 ccaaggttga aaacgaattg aacgtaaaga gcattaaaca agacctacaa aatcaaattg
    26401 aacaggataa aatgaaatat cagcttcaag aaaagagttt gcaggaaaaa tatgatactt
    26461 tactacagac caaagatgaa cagattgctt actataaaga ttttaaatta aaacagtcta
    26521 ctaaaatgat tggtgaatct ttagaacaac attgtgaaat tgaattcaat aagcttcgtg
    26581 cgactggttt tcaaaatgca tactttgaaa aagataatga tgctcataca ggatctaagg
    26641 gtgactatat ctatcgtgaa ttagatgaga atggtgtaga aatcatttca attatgtttg
    26701 agatgaaaaa cgaaaatgat aaaacagcca caaaacataa gaatgaagat ttcttaaagg
    26761 aattagataa ggatcgtaat gaaaaaaatt gtgaatatgc cgttcttgtt tctatgttag
    26821 aaccagaaaa tgaattatat aacacgggta ttgttgataa atcgcatcgc tatccgaaga
    26881 tgtacgtaat tcgtccacag ttctttattc ctatgatcac cattttaaga aatgccgcat
    26941 tcaattctat gcagtataaa aatgaattac aattgctgcg taatcaaaat attgatatca
    27001 cacacttcga agagaacatg aatgatttca aagaaaagtt ctcaagaaat tataggattg
    27061 catcggataa attcgcaaaa gctatcgatg aaatcgataa aactattttg catcttcaaa
    27121 agacaaaaga agatttatta tcaagtgaaa gacaattgca attggctaat aataaagcag
    27181 aggatcttac aatcaagcgt ttaactcgta acaatcctac aatgaagcag aagtttgatg
    27241 aattaaagaa agataacaaa taaaggacat ttccaatagg agatgtcctc ttttttaaaa
    27301 catattcatg aataatgaaa tgataatcca aagaatcgta aatattaaat ataaaaggtt
    27361 aggaatttgc ttaaagaatc ttgcatacca attctcattt gtcttctcct ctttaataaa
    27421 tgcattcact tcctttgatt ttaatattat tgtaatgaga ccataaatca caagggcaag
    27481 cactaaaact gtaatcaaaa tatgatttga atatgctgac attagagcta aaaagttatt
    27541 tcc
//
LOCUS       DBMDWL010000006        26799 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_692_length_26799_cov_3.457338, whole genome shotgun sequence.
ACCESSION   DBMDWL010000006 DBMDWL010000000
VERSION     DBMDWL010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 26799)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 26799)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26799
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_26799_cov_3.457338"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="ACZPDN_00790"
     CDS             complement(<1..909)
                     /locus_tag="ACZPDN_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297194.1"
                     /translation="MIQIPDNSTILAPATLHLSLYKEILKQKKDCLGIQVLTLSSWLS
                     SFYHGQTKSDIEILYLYKEALKNTSLSNAFYSSKEDYDFLGACLDFIKMAKTYQIHNF
                     PCSTQKEKDLNEILNLLYPIELKEDQTQDVLSSLPDLENIYILKKEYSQLDNYWIQVL
                     MDHGAKWLGENQLCQTHYYSVANARKQMEVLANLIIENDYLADDIFVALNNASDESVL
                     TQILTAHKIPFTTMQETHITSIYNEWISYLTWIKDMDLKSFINLIQTLYPSNNYLIQY
                     YTLFPEKFLKFEPHLSIISYEENEIID"
     gene            1013..2374
                     /locus_tag="ACZPDN_00795"
     CDS             1013..2374
                     /locus_tag="ACZPDN_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013776.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1297195.1"
                     /translation="MFVGRKKELDALNSRYKSNRKEFGVLYGRRRIGKSSILNEFLKG
                     KKGILFQAKQDNSYGNLKSFSYEVDHLLNLPDTFVFASWQHAFKSIQEYAKNERFVLV
                     IDEYPYIVSQDSSFSSVLQEFIDHASDNMMVILSGSDIGFLQKEIQNHASPLYKRRTF
                     EMEIKKMNFMDAKEFVEMEDVESQANYLCLMSGYPYYLSTIDHTKTFYENIQYLLFNE
                     FGTFFNLPDQLLSNSTNVQDVYNSILVSISKRNRTIKTISEDIHEAEAKVSKYISTLI
                     KSEIVEKKNMYNGNKRTIYYDISDSMLKLWYTLIFENQEKIKINGNLVFERNKEKLDS
                     FLSFEFENLSILYMDMLNEKGELEDIFPHIQNYKVDNSKLNRSIEIDGITSTKEYLLV
                     MDCKYRNKKYTKDMYEHLKESASIFPDKLKRVYYIFSKVGFNENMIEDKNIHLFDLKK
                     MFE"
     gene            complement(2414..3784)
                     /locus_tag="ACZPDN_00800"
     CDS             complement(2414..3784)
                     /locus_tag="ACZPDN_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1297196.1"
                     /translation="MQYDIIIIGAGPGGIFSAYELLKNKPELKIGVFEAGAPLEKRKC
                     PIDGKKIKSCIHCKSCAIMNGFGGAGAFSDGKYNITNEFGGNLYEYVGEQEAIDLMEY
                     VDEINCKMGGEKTRLYANSDAAIAKKCLQNDLHLLKAKVRHLGTDINYVVLENLYNDL
                     KDKAEFHFFKHIDMVNKIEDGYEVVCGNETYTSTQCIISTGRSGSKWMESVCSNLGIE
                     TKSDRVDIGVRVELNADTFSELTDSLYESKIVYRSKKYQDRVRTFCMNPRGVVVNENT
                     NGIITVNGHSYEDPARFTNNTNFALLVSNHFTEPFSQSNQYGESIARLSNMLGGGVIV
                     QRFGDLIRGQRSTPSRIAQGFVTPTLKATPGDLSLVIPKRQLDDIIEMIYALDKVCPS
                     TASDDTLLYGVEVKFYNMQVKVDKNLETKHKGLFVIGDCSGVTHSLSHASASGVYVAR
                     HITENL"
     regulatory      complement(3791..3888)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            3971..4855
                     /locus_tag="ACZPDN_00805"
     CDS             3971..4855
                     /locus_tag="ACZPDN_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1297197.1"
                     /translation="MSKNRIHDLIMIILGNFVVACSVSFFILPNNILTGGVAGVAVAL
                     HPILPVDTVLMIDTLTIGLFILGALLLGKQFAMKSVISTIVYPVFVTGLSKVATMFPK
                     DTFVMPPYVATIYAGVIVGIGLGLVFRVNSSTGGMDILALILHKYFKIPEGTSVMIVD
                     GLTVLLGVVTHGLTPALIGIMSVFVCGVAIDKTVMLGMQSAKNVMIISTEWKQIRRIL
                     LETVDRGVTILDGSGGYTQAPKPVLMCVIKQKQYPLLESSVLKIDSKAFIIVNDVHQV
                     HGAGFTSKHVVETNETTY"
     gene            4839..5363
                     /gene="rnmV"
                     /locus_tag="ACZPDN_00810"
     CDS             4839..5363
                     /gene="rnmV"
                     /locus_tag="ACZPDN_00810"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HIR1297198.1"
                     /translation="MKQHIKEIIVVEGKNDTNTLQSFFDCDTIETGGDQVNEKTIERV
                     RQAQKTRGVIIFTDPDTPGEHIRRLIGGSVPGCKHAFINKEKAKTPKKVGVEHANRED
                     LWDALCNYVTFENKEEVLSWQDFIDLGLVGNKALRFQVCESFHIGPCNAKTCFKRLNQ
                     MKMTRKEIEERIFS"
     gene            5360..6229
                     /gene="rsmA"
                     /locus_tag="ACZPDN_00815"
     CDS             5360..6229
                     /gene="rsmA"
                     /locus_tag="ACZPDN_00815"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1297199.1"
                     /translation="MKQAIATVKKTKEIQEKYKVFTKKSFGQNFIIEPGVVEKIANAA
                     IQSRDELVFEIGPGIGALTQFLCEKSDQVVAFEIDERLPVVLENEIGFDHLQIVLEDI
                     LKVDIDEKIREFRKPGQKVVFASNLPYYITTPILFKLFEANEEIERITVMMQKEVGER
                     FLAHENDKEYNALSVITQYRCDVKKVMDVSRRVFWPSPNVDSMVIQFSFHHKYHLEDE
                     NYFFDLVKACFTQRRKTIYNNFQNFVKDKALAKQLLEQVNMDPSTRAQQCTLEDFIRL
                     YEVTYANTGKGKN"
     gene            6201..7034
                     /gene="ispE"
                     /locus_tag="ACZPDN_00820"
     CDS             6201..7034
                     /gene="ispE"
                     /locus_tag="ACZPDN_00820"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1297200.1"
                     /translation="MQIQARAKINLALDVVERMDNGYHSLDMIMAPISIYDEIEIEKS
                     DCDMVECEGMKLPENNTVSKMIQVLKDTYHLDSSYKVSIKKHIPAQAGLGGGSADAAA
                     VCKAILKMEGIEETEENLYEITKQVGADVPFCIHDKWARVKGIGEKITPIESDWKFDV
                     VVVKPDFGISTANAFSKWKESRPFHPDVDLVESSIREENMDLLYQTMANALEPIAFEI
                     EPELNQVKMDMEDAGLVRILMSGSGSSMLGFSVDEDVILNAYEQLKEKYDFVKIIHVG
                     G"
     gene            7036..7242
                     /locus_tag="ACZPDN_00825"
     CDS             7036..7242
                     /locus_tag="ACZPDN_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HIR1297201.1"
                     /translation="MAASVISYFVFFACFGLSFYAISSIQFEKICSVKNPKKVQILMF
                     LLSLCLAYVSSQAILQLTIYNGFA"
     gene            7229..8167
                     /locus_tag="ACZPDN_00830"
     CDS             7229..8167
                     /locus_tag="ACZPDN_00830"
                     /inference="COORDINATES: protein motif:HMM:NF018435.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR1297202.1"
                     /translation="MALLDKWIALDIGAYLARFYDFSTQKEIVLKTIIAQKGSEVLGV
                     SDQAIAYLYKDLDTIQIQYPISHGQILHSIEPLIHEGLDKLHAYDGLLRPSVLVSVPS
                     ELSQRQRELWQQAFLNCGVRKVEFISNLEVLQEENPCFLVHSGHSYTEIHICAYDKVL
                     VSKTIYYAGAQVDEQIQRIVYMKTGYFITKVDAAHLKEMASDAFWQQKNTLLMCYGFD
                     SRQNLHPIQIESSIVWPAIEMVSSQISLWVKHCFDTLPASLQEYFLMHGIELSGGMAD
                     CFGPSQLISQNISCPVLCTKRGQYDMIDALKGVKSA"
     gene            8164..9231
                     /locus_tag="ACZPDN_00835"
     CDS             8164..9231
                     /locus_tag="ACZPDN_00835"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR1297203.1"
                     /translation="MIDYRMILLPFLISLILTPVVKQYSIYCGAYAKENKRTVHHGKI
                     SRIGGVAIYLSFIITMAIFVKADLQIKGLVIGSSIMFFTGLIDDLIDIKPLVKLTLEV
                     IAALVLIYFGISVDIIRLPLGIQIDTGIISFVFTIIWVAGITNAVNLIDGLDGLAGGM
                     SVIVLVVIGSIAIIERRLDLLSIAFILASGTFGFLAYNAHPASIFMGDCGSLFLGFMI
                     SAISLLGFKSSTILTLALPILLLMLPIIDTLSAILRRTLKGMKFMEADKSHIHHLLMR
                     QFGHRNTVIIMCGLTALFGLSAFAYMINRNIGFFVMVAILLAVEIFIEKSAMISEKFH
                     PLIGLWHRIQRKSRKIFKKVG"
     gene            9282..9830
                     /locus_tag="ACZPDN_00840"
     CDS             9282..9830
                     /locus_tag="ACZPDN_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297204.1"
                     /translation="MEPNEWQKKKNNKTTEYTQRITSQARIAEEKLLSGLNESEVYDY
                     MLSKLNFCQRKQMYSSLEAKVYVKVGDICFTDFGLAYLSEVGYQHFGLVLAMKHGKIF
                     VVPMSGNSSAYHKAYDPILRAGKRHLMRLPEIEGLNKSSVLFLNDAKWINSARVIDVK
                     AHIDPNSYLFDKIKKCVLQMML"
     gene            9838..11022
                     /gene="tilS"
                     /locus_tag="ACZPDN_00845"
     CDS             9838..11022
                     /gene="tilS"
                     /locus_tag="ACZPDN_00845"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1297205.1"
                     /translation="MSKCVVACSGGPDSMALLDQLNKQGKDIVVAHVNYKHRDTADRD
                     ENIVKDYCEKYDIPVRVCYPVHEKGNFQAWARDVRYAFFEEVADAFDAKILYVAHQMD
                     DVIETYLFQKNRNMICDWYGLKEKSIRHGYQIIRPLLNFTKSELQQYCNENGVSFGID
                     ESNLTNHYTRNVIRHTQIEKMSRDEKEAWILKIQNENEVWQIKRKAIEEFFKDWNKDI
                     DSLLDQEDAWLYLDTFLFHSLHHHFSKKYMEELCAQLKRNVLIEIENYLLERHGGKLY
                     FMPSPKDVYYKLDELEFKDYADFVIKNEGKTIEMFSVDASDFPLVIRRVRANDTIQMR
                     FGNKNVHRFFVDRKISKIYRKYWLVVENNVGNVIFVPGLGCDVGHYSQNQQFYFKING
                     LD"
     gene            11069..11611
                     /gene="hpt"
                     /locus_tag="ACZPDN_00850"
     CDS             11069..11611
                     /gene="hpt"
                     /locus_tag="ACZPDN_00850"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR1297206.1"
                     /translation="MIAQDIEKVLLSEEEIENRCVELAHQIEADYKESGDVPIVVGLL
                     KGSVPFMAELIKRFEFPCEIDFMSVSSYDGTESIGDVRILKDMDLSCKNRSILVVEDI
                     IDTGRTLVEVKKMFKNKGCLDVRCVTLLDKPSRRVCEIEADYVGFEVPNEFVVGYGLD
                     YNQKYRNLPFIGVLKPDIYQ"
     gene            11622..13577
                     /gene="ftsH"
                     /locus_tag="ACZPDN_00855"
     CDS             11622..13577
                     /gene="ftsH"
                     /locus_tag="ACZPDN_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1297207.1"
                     /translation="MKKWLNYLPTIFILSIVLSLVFYSSQGSTVQMNYTQFEKIVDEV
                     DFKKSSMTISSTVIHLEGTYEQNSKTVGYKVIVPRTEKNIEKLEKSLQRNGGKLTVED
                     PNQGSVWISILSQIIPFLIIAVFFYFMFAKMGGGGNNKAFEFAKSKARVESNVKVRFK
                     DVAGCEEEKEEVKEIIDYLRSPKKFTDMGAHIPKGILMVGPPGTGKTLLAKAVAGEAN
                     VPFFSISGSDFVEMFVGTGASRVRDMFKTAQKSAPCIIFIDEIDAVGRQRGAGMGGGN
                     DEREQTLNQLLVEMDGMTDNAGIVVIAATNRPDVLDPALLRSGRFDRRVTVSLPDIKG
                     REAILHVHARNKKLASDVSLANLAKRTPGFSGADLANVLNEGAILAVRKNEAKVTMTD
                     LDEAIDRVMMGPAKKSKKYTEKDKILVAYHEAGHAVIGLKLEDADMVQKVTIIPRGDA
                     GGYNLMTPREEKYFHRKSEFIAQITGLLGGRTAEEIQFGEISAGAVNDIEKLTEIAKN
                     MVRVYGMSSLGPIQYADPQGNVFLGRDYTKGSDYSAGVAAEIDKEVRAIVDECHENCR
                     KILTENKDLLDLIANNLYEHETLTNEEITNLMNYGQLTDPNVETSEEEEKAEQEVVQV
                     PEIPETPTGVDQKDLDDAFNELTKKKD"
     gene            13678..14562
                     /gene="hslO"
                     /locus_tag="ACZPDN_00860"
     CDS             13678..14562
                     /gene="hslO"
                     /locus_tag="ACZPDN_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1297208.1"
                     /translation="MKDQLLKALVLNEHVRLYIVRTTDLVQEAQDRFDLHPCACAALG
                     RTLSVASMMGAMLKSEEEMLSITINGHGPIGSIVVDAYANGNVRGFVSNPHVEDVLIR
                     PGKLNVGAVVGTDGTLTVTKDLSMEENFSGSVELQSGEIGDDFAYYFTLSEQTPTAVS
                     CGVLIGTDGRVASAGAMILQMLPDATETDISICEHVLEGLKPMSTLIQEYDDSSLEAL
                     ANDMFEDAKILTTQPVAYDCPCSKVRMARALGTIKKSDVLEMADKEHGCDITCNFCNE
                     SYHFTEEELRAIASKMHD"
     gene            14555..15520
                     /gene="dusB"
                     /locus_tag="ACZPDN_00865"
     CDS             14555..15520
                     /gene="dusB"
                     /locus_tag="ACZPDN_00865"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1297209.1"
                     /translation="MIKIGNVEIENPVVIAPMAGISNIAFRRIAKRFGAGLVCNEMVS
                     DKALYYDSLKTKQMCAIDENEHPVSFQLFGHDIDTVVYAAKYLDTQTDCDIIDFNMGC
                     PVNKVIKAQAGSFLMKDIDYAKELVSNIVQNVNKPVTVKMRLGFDTYHINCVEMAKAM
                     ESVGVSAVAVHGRTRNQMYEGKADWSYIKQVKDAVSIPVIGNGDVRSVADFHRMMNET
                     GCDAVMLGRGIVGNPFLIQECVDSITGQKHEFSMEDRIEICMDHARGLVNTKGEKVAI
                     REMRGLASWYLKGLPSSRIFKNEMSQMNTLEDLENILHQFMEVQR"
     gene            15625..17451
                     /gene="glgB"
                     /locus_tag="ACZPDN_00870"
     CDS             15625..17451
                     /gene="glgB"
                     /locus_tag="ACZPDN_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1297210.1"
                     /translation="MDQRKTKAFFEGRDIHAYNIFGAHLEGDGVRFRVYAPNAKNISV
                     IGTFNNWDDQASKMKKDKRGIWECFVQGAKKQDSYKYRVWQANGELVDKMDPYAFYSE
                     LRPNTASIISDLSYDGWTDKEWLNQRNKGFDSPVNIYEMHVGSWKKEDENEEFVQYHE
                     IEKDLIEHCKKHHFTHVEVMPLCEYPFDGSWGYQCTGYFSSTSRYGTNHELMHFVNAL
                     HEAGIGVIMDFVPVHFVKDNYALGMFDGTPLYEYSKPEDANSEWGTANFDLWKEEVRS
                     FLMSAANFWIDVYHVDGLRMDAISNAIFWHGNKNNGVNEGACDFMKRMNHLLNEEHQG
                     KIMLIAEDSSDFPNVTKPSYAGGLGFDYKWDLGWMNDTLSYLKRDPIYRKWHHHDITF
                     SMAYFYSERFILPFSHDEVVHGKATIVDKMWGSYEDKFAQARALYVYMFAHPGKKLNF
                     MGNEIGQLREWDEQKSCDWFLLDYPMHDAFKRLCEDLGKIYTENDAFFNQDYDPSRFS
                     WIDADNVGQNTFSFIRRGSKKDYVIIMNFSTNPYDHQQFGVPCKGKYKEILNTEWDKY
                     NGNLKDHTPQEVQAMRLARHNQPYMIEVNVPSFGAMIYEVEK"
     gene            17529..17900
                     /locus_tag="ACZPDN_00875"
     CDS             17529..17900
                     /locus_tag="ACZPDN_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297211.1"
                     /translation="MKKLDVEFELFRKRVYNLQVRDFYLLLMNRGCDMDTLRVLFSDV
                     DDKLSIKLDGMFYYAQSDMIQEVLERVIKQEKTNARMDCFKRLYEKGMDVFEVLELLG
                     ISQNDIEMINEAAYVWQDMHL"
     gene            17949..18797
                     /locus_tag="ACZPDN_00880"
     CDS             17949..18797
                     /locus_tag="ACZPDN_00880"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1297212.1"
                     /translation="MYAQKGDNLNIMENKELALHAKNIRRNIVKEVYSAQSGHPGGSL
                     GATDILTELYFEEMDINEENLNTLNRDRFVLSKGHASPALYAVLAEKGFLPEEELTTF
                     RAINSRLQGHPNMNYVEGVDMSTGSLGQGISCAVGMALANKLDKNDHRIYTVLGDGEC
                     QEGQVWEATMSAAHYKLDNLCIIVDNNNLQIDGHVDEVMSVYPLDKKFEAFNCHVINV
                     DGHDFDQLRAALKEARETKGMPTVIVAKTIKGKGVSFMEDQQGWHGVAPNEEQYHAAM
                     KELEAE"
     gene            18797..19711
                     /locus_tag="ACZPDN_00885"
     CDS             18797..19711
                     /locus_tag="ACZPDN_00885"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1297213.1"
                     /translation="MAKVATRVAYGDALKELMVENENVVVLDADLAGSTKSGEAKKVD
                     PARHFDMGIAEGNMMSVAAGLAASGKIAYASTFAMFATGRGFEQIRNSIGYPQLNVKI
                     CASHAGISVGEDGASHQCIEDVSLMRGIPGMKVIVPCDYNEAKQACKAVAEIDGPCYV
                     RLGRSGVESVNGDDYKFELGKGVVLQKGEKVALVATGLMVQEALEAAKKMETAPTVVN
                     IHTIKPIDKELIVELAKTHDKIVTCEEHSIIGGLGSAVAEVLAEAGTACKLVRVGVQD
                     VFGESGKPAQLFAKYKIDADAIIEAATK"
     gene            19713..20306
                     /locus_tag="ACZPDN_00890"
     CDS             19713..20306
                     /locus_tag="ACZPDN_00890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1297214.1"
                     /translation="MAHYFTNDIVKDAPEEITIHFRDFTYKLNSNAGVFSKDKLDEGT
                     RILLETVLDNQSEPVSCLDLGCGIGPIALILMEYWKHTEMTMIDVNQRACQLADSNMK
                     KYRRKAKIICQSGINEGQYACILLNPPIRTGKAMIYSLFDQCMEHLATDGHFWIVMRK
                     QHGAQSAIHYLQEKGYEVEKMAREKGYWVMKISKAGN"
     gene            complement(20534..20710)
                     /locus_tag="ACZPDN_00895"
     CDS             complement(20534..20710)
                     /locus_tag="ACZPDN_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297215.1"
                     /translation="MIDELFPNEPEENYMMHCPECNHKMIVKNNSTTKDKYKMLAITI
                     VLFVVVDNPQIMTI"
     gene            20852..22891
                     /locus_tag="ACZPDN_00900"
     CDS             20852..22891
                     /locus_tag="ACZPDN_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HIR1297216.1"
                     /translation="MLPIAVLPVAGIFLGVGSSLTNETTIAALHLELVLGQGTFLYSV
                     LILLKQVGKVLFDNLPLIFAVSIALGMSKRRKEVAALAAIIAFFVMHATINTLLCLDG
                     SIVNGVVSNYILDGSITSVCGMLSLEMGVFGGIVVGLGVSYLHNHFYQIQLPNVISFY
                     EGERFVPIICAITYVFIGVLMYFIWPPLQHGVYNLGQFISDSGYFGTFIYGIIKRSLV
                     PFGLHHVWYMPFYQSALGGVQMVNGSMVSGAQNIFFAQLSDPSVTHFSVDATKFFSGE
                     FIFMIFGLPGAALAMYQCANPEAKKKTASLLLSAALTSALTGITEPIEFSFLFVAPLL
                     YVVHVFLAATCFVVAQALQVAIGFTFSAGLLDFTLFGILQGNAKTNWITVVLLGLVYF
                     FVYYGVFKFLIVKYDLKTPGRDESIKVFDKKKYDFSFDKSLIDPRSQMIVQGLGGRSN
                     FTDLDCCITRLRATIKDDSLINEGLLKKSGAAAIMCQPNAIQIIYGPQASNIKTKLDE
                     YMLNVPESYDFEDKEVVLETKTQFELECLVNGEVMPIEAASDSMFAHKLMGDGIMIRP
                     YDGVVVAPCDGVISMIYPTKHAIGITIDDNTQILIHFGTDSAKLNGKGFELLVRPNQE
                     VKAGDVLWNADLKYIKDNALDENIIVVFTKIKDGAELEKKYGKMQCHDMMLRVKG"
     gene            22891..23730
                     /locus_tag="ACZPDN_00905"
     CDS             22891..23730
                     /locus_tag="ACZPDN_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIR1297217.1"
                     /translation="MDMCEIVKILNHNTILVLKDNEEIIVMSKGIGFGKKQGEQVDVP
                     RNAKQYKMQKSYQAKQKSNNIFDYIDPMYIEISSEIITLTKERFGKVEHDILLSLADH
                     IYFAVKRIKEKDFPSNPFNMDIQLMFPDEYSVALKAKDIIEKYTHEEINADEIAFITL
                     HIHSAISVNKVGQSMEVMRVIREFFKKLQADLNIRIDANSLSYIRLMNHIKFLLLRLN
                     NNEELQMDITEFTKEKFPFAYEQARVLCEQLSHVLRKDLPDSELGYLALHLERILSST
                     SIS"
     gene            23931..24911
                     /locus_tag="ACZPDN_00910"
     CDS             23931..24911
                     /locus_tag="ACZPDN_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1297218.1"
                     /translation="MVGIILASHGEFANGIFQSGAMIFGEQANVKPCTLMPSEGPEDI
                     RKKMEDAIASFENQEEVLFLVDLWGGTPFNQASALLDGHEDKWAIVTGLNLPMLIETY
                     ASRFSMEKAHEIAAHVMQTAQEGVQINPKELEPKEEKKVEVSAAPQGAIPEGTVLGDG
                     HIKYGLARIDTRLLHGQVATAWTKTVGPNRIIVVSDNVAHDDLRKSMIEQAAPPGVKA
                     NVVPIDKMIQVAKDPRFGATKAMLLFETPQDALRAIKGGVDIKELNLGSMAHSQGKVV
                     CTKAVSMGSDDVKTFDELLAMGIKIDVRKVPTDSPENFDEILKKAKTELK"
     gene            24930..25748
                     /locus_tag="ACZPDN_00915"
     CDS             24930..25748
                     /locus_tag="ACZPDN_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HIR1297219.1"
                     /translation="MSAISIILVIIVAFLAGMEGILDQFEFHQPLVACTLIGLVTGHL
                     KEGVMLGGSLQLMALGWANIGAAVAPDAALASVASAIIMVLGLNGGTTDTTTAISTAI
                     AVAIPLSVAGLFLTMICRTIAIPIVHFMDAAAEKGDFGAIERWQIVGICLQGVRIAIP
                     AAALCFVPSSVVTGVLNAMPAWLSGGMTVGGGMVAAVGYAMVINMMATKETWPFFALG
                     FVFACVSEFTLIALGVIGVVIAIVYLGLKDAAKNSGGGSNTGSGDPLGDILNDY"
     gene            25772..26689
                     /locus_tag="ACZPDN_00920"
     CDS             25772..26689
                     /locus_tag="ACZPDN_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1297220.1"
                     /translation="MAEKITLTKQDRKKVWWRHQFLQGSWNYERMQNGGWCYSIIPAI
                     KKLYSNKEDQIAALKRHMEFYNTHPYVSSPVMGVTLALEEEKANGENVDDTAIQGVKV
                     GMMGPLAGVGDPVFWFTMRPILGALGASLALSGNLLGPILFFVLWNVIRLAFMWYTQE
                     FGYNVGTKIAKDMSGGLLGKITQGASILGMFIIGALVQRWVSISFTPVVSSVAQSEGA
                     YIDWSQIKGNADGIKSALEQYSSLGATGLNQIKVTTLQQNLDSLIPGLAALLLTLLCC
                     KLLKKKVSPITIIIALFVVGIIGRVIGIM"
     gene            26708..>26799
                     /locus_tag="ACZPDN_00925"
     CDS             26708..>26799
                     /locus_tag="ACZPDN_00925"
                     /inference="COORDINATES: protein motif:HMM:NF017886.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HIR1297221.1"
                     /translation="MAQSLNSKVDLTIKATSFAGLSSNGNVMVG"
BASE COUNT         8687 a         3505 c         5778 g         8829 t
ORIGIN      
        1 atcgatgatc tcattttctt cgtaagaaat aatacttagg tgcggttcaa actttagaaa
       61 tttttccggg aataatgtgt agtattgtat tagatagttg ttgcttggat ataaagtttg
      121 aatgaggttt ataaaacttt taagatccat atcttttatc catgttaagt aactgatcca
      181 ttcattataa atcgatgtaa tatgtgtttc ttgcatagtt gtgaatggaa tcttatgagc
      241 tgtaagaatt tgtgttagaa cactttcatc actcgcatta tttaaggcaa cgaaaatatc
      301 atccgctaaa taatcgtttt cgataataag attggcaagt acttccattt gttttctggc
      361 attggcaaca ctataataat gggtttgaca taattggttt tcacctagcc attttgcccc
      421 atgatccatt aatacttgaa tccaatagtt gtctaattgg gaatattctt tttttagaat
      481 atagatattt tctaaatcag gtaaacttga aagaacgtct tgagtttggt cttccttaag
      541 ttcaatggga taaagcagat ttaagatttc atttaaatcc ttttcttttt gggttgaaca
      601 aggaaaatta tggatttgat aggttttggc cattttaata aagtctaggc aggcacctaa
      661 aaaatcatag tcttctttac tggagtagaa ggcatttgaa agggatgtgt tctttaaagc
      721 ttctttgtat aaataaagaa tttcaatatc ggattttgtt tgtccatgat aaaaagaaga
      781 tagccagctt gataaagtta aaacttgtat gccaaggcaa tctttttttt gttttaaaat
      841 ctctttgtat aaagataaat gtagggtagc cggagctaat attgtactgt tgtctggaat
      901 ttgtatcata aaattatcac ctagattatt ataccatgtt gcgtatattt tcattcaatg
      961 atattattaa ctcgaaagtt aataactgtg gagttaataa agtggaggac ctatgtttgt
     1021 tggtagaaaa aaggaattag atgcattaaa tagccgatat aaaagtaaca gaaaagaatt
     1081 tggtgtattg tatggaagaa gacgaatagg gaaatcatcc atattaaatg aatttttaaa
     1141 gggcaaaaaa ggtatattgt ttcaggctaa acaagataat agttatggaa atttaaaatc
     1201 tttttcttat gaagtggatc atttgttgaa tctaccagat acatttgttt ttgcgagttg
     1261 gcaacatgcc tttaaatcga ttcaagaata cgcaaagaat gaacgttttg tgttggttat
     1321 agatgagtat ccgtatatag taagtcaaga ttctagtttc tcttccgttt tacaagaatt
     1381 tattgatcat gcgagtgata atatgatggt catcttatcg ggaagcgata taggattttt
     1441 gcaaaaagaa atacagaatc atgcgagtcc attatataaa agacgtactt ttgagatgga
     1501 aatcaaaaaa atgaatttta tggacgcaaa ggaatttgtg gagatggagg atgttgaaag
     1561 ccaagctaat tatttgtgtt taatgtcggg atatccatat tatttatcga caattgatca
     1621 tacaaaaaca ttttatgaaa atattcaata tttattattt aatgaatttg gtacgttttt
     1681 taatttgcca gatcaacttc tatccaattc aacgaatgta caggatgtat ataattcaat
     1741 attagtatcg atttcaaagc gaaatcgtac aataaaaacg attagtgaag atattcatga
     1801 ggcagaggct aaagtctcta aatatatttc aacattgata aagagtgaaa ttgtagagaa
     1861 aaagaatatg tataatggaa ataagcgaac gatatattat gatatatcgg attcgatgtt
     1921 gaaattatgg tacaccttaa tttttgaaaa tcaggagaag ataaaaataa atggaaatct
     1981 tgtgtttgaa cgaaataaag aaaaattaga ttcatttctt tcgtttgaat ttgaaaattt
     2041 atcgatttta tatatggata tgttaaatga aaaaggtgaa ttagaagaca tttttccaca
     2101 tatacagaat tataaagttg ataactcgaa gttaaacagg tcaattgaga tagatggtat
     2161 cacaagtact aaagaatatt tgttggtgat ggattgtaaa tatcgaaata aaaaatatac
     2221 aaaggacatg tatgaacatc taaaagagag tgcctctatt tttccagaca aattaaaaag
     2281 agtatattat atcttttcga aagtaggatt caatgaaaat atgattgaag ataaaaatat
     2341 acatttgttt gacttgaaaa aaatgtttga ataaatgaaa aaaagtggat catttgatcc
     2401 actttgtgtt gtattataga ttttctgtaa tgtgtctagc gacatataca ccagatgcac
     2461 tggcatgact taatgagtgt gttacacctg aacagtctcc gataacgaat agtcctttgt
     2521 gttttgtttc taagtttttg tctactttta cttgcatgtt gtagaattta acttcaacac
     2581 catatagtaa tgtatcatca ctagctgttg atggacatac tttatcaagg gcataaatca
     2641 tttcgataat gtcatctaat tgacgtttag gaataactag agataagtct cctggtgtag
     2701 ccttcaatgt aggtgttacg aatccttgtg caatacgact tggagttgag cgttgtccac
     2761 gaatcaaatc accaaagcgt tgtacgataa caccaccacc taacatattt gataagcggg
     2821 caatactttc accatattgg ttactttgtg agaatggttc tgtaaagtga tttgatacta
     2881 atagggcaaa gtttgtgtta tttgtgaaac gagctggatc ttcataactg tgaccattta
     2941 cagtgatgat tccgtttgtg ttttcgttta cgacaacacc tctaggattc atgcagaatg
     3001 tacgaacgcg atcttggtat ttctttgaac gatatacgat cttagattca tacaatgagt
     3061 ctgttaattc tgagaatgta tcggcattta attcaacacg aacaccaatg tctacacgat
     3121 cactttttgt ttcaatacct agatttgaac atacagattc catccattta cttcctgaac
     3181 gtcctgtaga gatgatacat tgtgtagatg tatatgtttc atttccacaa actacttcat
     3241 atccatcttc aattttattt accatatcga tgtgtttaaa gaaatggaat tcggctttgt
     3301 cttttaaatc attgtataag ttttcaagta caacatagtt aatatctgtt cctaagtgac
     3361 gtacttttgc ctttaataag tgaagatcat tttgaagaca ttttttagcg atagctgcat
     3421 ctgagtttgc gtataaacgt gttttttctc cacccatttt gcagttgatt tcatccacat
     3481 attccattaa atcgatagct tcttgttcac ctacatattc atataggttt ccaccaaatt
     3541 catttgtgat attatatttt ccatcactga atgctccggc tccaccaaaa ccattcatga
     3601 tagcacatga tttacagtga atacatgatt tgattttctt tccatcgatt ggacattttc
     3661 ttttttctaa tggagcgccc gcttcaaata caccaatttt taattcaggt ttgtttttta
     3721 ataactcata tgctgagaaa atacctccag gcccagcacc aataataatg atatcgtatt
     3781 gcattttatt ttcctcctgc ttgaaggcta tagtctacta ttttcggtag taggtaagaa
     3841 cgttcaacca tatcatgaac ttatataacc cggactatta tacggataaa gtttatttta
     3901 tgcaagtaaa taattttaaa atgatacata aaaaaatgct ataatcatgt aggctagtag
     3961 aggtgtgcat atgagtaaga atagaataca tgatttaata atgattatac ttggaaactt
     4021 tgtggtcgca tgttcggttt ccttttttat acttcctaat aatatattaa cgggaggcgt
     4081 tgccggtgta gccgtcgcat tacatccaat ccttccagtt gataccgtct taatgattga
     4141 tactttgaca attggtctat ttatattggg agctctttta ttaggaaagc agtttgcgat
     4201 gaaatctgtg atttctacaa ttgtatatcc ggttttcgta acaggcttat ctaaggttgc
     4261 tactatgttt cctaaagata catttgtgat gccaccttat gtagccacaa tctatgccgg
     4321 agtgattgta ggtattggct taggattggt gtttagagtc aattcaagta cgggtggtat
     4381 ggatatatta gcattgatct tacataagta ttttaagatt cctgaaggaa caagtgtaat
     4441 gattgttgat ggtttaactg tattattagg tgttgtaaca cacggattga caccagcttt
     4501 gattggtatt atgtctgtat ttgtttgtgg tgtggccatt gataaaacgg ttatgcttgg
     4561 catgcaatcc gcaaaaaatg ttatgattat atcgacagag tggaaacaga ttcgtcgtat
     4621 tctattggaa acagtggatc gtggtgtaac gattctagat ggaagtggtg gatatacaca
     4681 agcacctaaa cctgtgttga tgtgtgttat taaacagaaa caatatccat tattggaatc
     4741 aagcgtctta aaaattgatt caaaagcttt tattattgtg aatgatgtac atcaggtaca
     4801 tggtgctggt tttacatcaa aacatgtggt ggagacaaat gaaacaacat attaaggaaa
     4861 ttattgtcgt tgaaggaaaa aatgacacaa atacgttgca gtcttttttt gattgtgaca
     4921 cgatagaaac aggtggggat caggtcaatg aaaaaacgat tgagcgtgta agacaagctc
     4981 aaaagactag aggagtcatt atctttacgg atcctgatac gccaggagaa catattcgta
     5041 gattgattgg tggtagtgtg ccaggatgta agcatgcgtt tattaataag gaaaaggcaa
     5101 agacaccaaa aaaagtaggc gtagagcatg cgaacagaga agatttatgg gatgccttat
     5161 gtaattatgt aacgtttgag aataaagaag aagtattaag ttggcaagac tttattgatt
     5221 taggtttagt tggaaataaa gctttacgtt ttcaagtgtg tgagtctttt catattggtc
     5281 catgtaatgc gaaaacatgt tttaagcgtt taaaccagat gaagatgaca agaaaagaga
     5341 tagaagaaag gattttttca tgaaacaggc gattgcgaca gtaaaaaaga caaaagaaat
     5401 tcaagaaaaa tataaggttt tcacaaagaa aagttttggg caaaacttta ttattgaacc
     5461 tggggttgtt gaaaaaattg cgaatgcagc gattcaatca agagatgaat tggtttttga
     5521 gattggtcct ggaattgggg cattaactca atttttatgt gaaaaaagtg atcaggttgt
     5581 ggcttttgaa attgatgaaa gattaccggt tgtattagaa aatgagattg gttttgatca
     5641 tcttcagatt gtgttggaag atattctaaa ggtggatatt gatgaaaaga ttcgtgaatt
     5701 tagaaagcct gggcaaaagg ttgtatttgc gtccaatctt ccttactaca tcacaacacc
     5761 aattttattt aagttgtttg aggcaaatga agagattgaa agaattacgg ttatgatgca
     5821 aaaagaagtg ggagagcgtt tcttggcaca tgaaaacgat aaggaataca atgcgttaag
     5881 tgtaattacg caatatcgtt gtgatgtgaa aaaagtaatg gatgtatctc gtagagtatt
     5941 ttggccaagt ccgaatgtgg attcaatggt gattcaattc tcgttccatc ataagtatca
     6001 tttagaagat gaaaattatt tctttgattt agtgaaggct tgttttacgc aaagaagaaa
     6061 aacaatttat aataattttc aaaattttgt gaaagataag gctttggcaa agcaattgtt
     6121 agaacaagta aatatggatc caagtacacg tgcacaacaa tgtacattgg aggattttat
     6181 tcgattatat gaggtgactt atgcaaatac aggcaagggc aaaaattaat ttggcattag
     6241 atgtcgtaga aagaatggac aatggatatc attctttaga tatgatcatg gcacctattt
     6301 caatttatga tgagattgag attgaaaaaa gcgattgtga catggttgaa tgtgaaggta
     6361 tgaaattacc tgagaataat acagtttcaa agatgattca ggtattaaag gatacatatc
     6421 atttagattc tagttataaa gtatcaatca agaagcatat tccggctcag gcaggtcttg
     6481 gtggcggaag tgctgatgca gcggctgtgt gtaaagctat tttaaaaatg gaaggtattg
     6541 aagaaacaga agaaaattta tatgaaatca caaaacaggt aggggcagat gttcctttct
     6601 gtattcatga taagtgggca agggtaaaag ggattggcga aaagattacg ccaattgaga
     6661 gtgattggaa atttgatgtt gtggttgtaa aaccggattt tggaatttct actgcgaatg
     6721 cgttctctaa atggaaagag tctcgtcctt ttcatccgga tgtggattta gtagagtcga
     6781 gtattcgaga agagaatatg gatcttttgt atcagactat ggctaatgca ttagagccta
     6841 tcgcttttga aattgagcct gaattgaatc aggtgaaaat ggatatggaa gatgctggtt
     6901 tggttcgtat attgatgtca ggatcaggtt cgagtatgtt aggctttagt gtggatgaag
     6961 atgtgatttt aaatgcatat gaacagttaa aagaaaagta tgattttgtc aaaatcatac
     7021 atgtaggtgg ctaatatggc ggcttcagta atttcttatt tcgttttctt tgcgtgtttt
     7081 ggtttaagct tttatgcgat ttcaagtatt cagtttgaaa aaatatgtag tgtaaaaaat
     7141 cctaagaaag tgcagatatt aatgtttcta ttatcgcttt gtcttgcgta tgtatcaagc
     7201 caggcaattc tacaattaac aatctataat ggctttgctt gataagtgga ttgccctgga
     7261 tattggggcg tatcttgcac gattttatga tttttctaca cagaaagaaa tagttttgaa
     7321 aacgataatc gcacaaaagg gatctgaagt tcttggcgta tccgatcagg caattgctta
     7381 tttatataag gatctagata cgattcaaat tcagtatcca atttctcatg gtcaaattct
     7441 tcattcgatt gagcctttga ttcatgaagg acttgataag cttcatgcct atgatggttt
     7501 attaagacct agcgtacttg tcagtgttcc aagtgaattg tcacaaagac aacgtgagct
     7561 ttggcagcag gcatttttaa attgtggagt acgtaaggtt gaatttattt caaatttaga
     7621 agttttacaa gaagaaaatc cttgtttttt agttcatagt gggcattcgt atacggaaat
     7681 tcatatatgt gcttatgata aggtgcttgt atctaaaacg atttattatg ctggagcaca
     7741 agtagacgaa caaattcaaa ggatcgttta tatgaagacg ggttatttta tcacaaaagt
     7801 ggatgcggca catttaaagg aaatggcaag tgatgcattt tggcagcaga aaaatactct
     7861 gttgatgtgc tatggttttg attcaaggca aaacctacat ccaattcaga ttgaatcaag
     7921 tatagtatgg cctgccattg aaatggtttc atcgcaaatt tctttgtggg ttaagcattg
     7981 ttttgatact cttccagctt ctttacagga atatttttta atgcatggaa ttgaattgag
     8041 tggtggcatg gcagattgtt ttggcccttc gcaattaatt tctcagaata tttcgtgtcc
     8101 agttctttgt acaaaacgtg gacaatacga tatgatagat gcattaaagg gggtaaagtc
     8161 agcatgattg attatagaat gattttatta ccatttttaa tttctttgat tttaacacct
     8221 gttgtgaaac aatattcgat atattgtggt gcttatgcga aggaaaataa gcgtacagtt
     8281 catcatggaa aaatttcacg tattggtggt gttgccattt atttatcttt tattatcaca
     8341 atggctatat ttgtgaaggc agatttacaa attaagggat tggtaatcgg aagcagtatt
     8401 atgtttttta cagggctgat cgatgatttg attgatataa agccactggt aaaattgact
     8461 ttagaagtca ttgctgcttt agtattaatt tattttggga tttctgtaga tattattcgt
     8521 ttacctttgg gtatacaaat tgatacaggt attatttcat ttgtttttac aattatttgg
     8581 gtggcaggta ttaccaatgc agtcaattta attgatggat tggatggctt ggctggtggc
     8641 atgagtgtta ttgtacttgt tgtcattggt tctatcgcaa ttattgaaag acgtttagat
     8701 ttgttgagta tagcttttat tcttgcgtcg ggaacatttg gctttttagc ctataatgcc
     8761 catcctgcta gtatttttat gggagattgt ggatctttat ttttgggatt tatgatttca
     8821 gccatatcct tgcttgggtt taaatcgagt actattttaa ctttggcatt gccgatttta
     8881 ttgttgatgt tgccaattat tgatacgctt tctgcaattt taagacgtac tttaaagggt
     8941 atgaaattta tggaagcgga taagagtcat attcatcatt tgttgatgcg tcaatttggt
     9001 catcgaaata cggtgatcat tatgtgtggt ttaacggcgt tatttggttt aagtgccttt
     9061 gcgtatatga tcaatagaaa tattgggttc tttgtcatgg tagctatttt gttggcagtg
     9121 gaaatattca ttgaaaaatc ggctatgatt agtgaaaaat tccatccttt gattgggttg
     9181 tggcatcgta ttcaaagaaa atctagaaaa atttttaaaa aagtaggctg aaatgcctac
     9241 ttttaatatt attttcacat tggcttgtta gaatagggtt tatggaaccg aatgaatggc
     9301 aaaaaaagaa aaataataaa acaacagaat atacgcaaag gatcacatca caagctagaa
     9361 ttgcggagga aaaactttta tcagggttaa atgaaagtga ggtttatgat tatatgcttt
     9421 ctaaactgaa cttttgtcag aggaaacaaa tgtattcgag tctagaggca aaagtatatg
     9481 taaaggtagg agatatttgt tttacagatt ttggtttagc ttatttaagt gaggttggtt
     9541 atcaacactt tggcttagtt ctggcaatga agcatgggaa aatttttgta gtacctatga
     9601 gtgggaatag tagtgcatat cataaggcat acgatccaat cttgcgtgca gggaagcgtc
     9661 atttaatgcg cttaccagaa attgaggggt tgaataaatc aagcgtattg tttttgaatg
     9721 atgcaaaatg gatcaattca gctcgtgtga tcgatgttaa ggcgcatatt gatccaaatt
     9781 catatttgtt tgataaaata aagaaatgcg ttttacaaat gatgctataa taggcgtatg
     9841 tcaaagtgtg tagtggcctg ttcaggtgga cctgacagta tggctttatt agatcaattg
     9901 aataaacaag gcaaagacat tgttgttgcg catgtgaatt ataaacatcg agatacagca
     9961 gacagagatg agaatatagt aaaagattat tgtgaaaagt atgatattcc cgttcgtgtt
    10021 tgttatccgg tacatgagaa aggaaatttt caggcatggg caagagatgt tcgttatgct
    10081 ttttttgaag aagtggcgga tgcgtttgat gcaaaaatat tatatgtagc tcatcagatg
    10141 gatgatgtga ttgaaacata tttgtttcaa aagaatagaa atatgatttg tgactggtat
    10201 ggattaaaag aaaagagtat acgacatggg tatcaaatta ttcgtccctt attgaatttc
    10261 acaaagagtg aattgcaaca atattgtaat gagaatggcg tatcttttgg gattgatgag
    10321 tcaaatttaa ccaatcatta tacgcgtaat gtaattcgtc atacacaaat tgaaaaaatg
    10381 agtcgagacg aaaaggaagc gtggatatta aaaattcaaa atgagaatga agtatggcaa
    10441 ataaaaagaa aagccataga agaatttttt aaggattgga acaaggatat agattccttg
    10501 ttggatcaag aagatgcttg gctttattta gatactttct tatttcattc tttgcatcat
    10561 catttctcaa aaaaatatat ggaagaatta tgtgctcaat taaaaaggaa tgtgttaatt
    10621 gaaatagaga attatttatt ggaaagacat ggtggtaagt tgtattttat gcctagtcct
    10681 aaagatgtgt attataaatt ggatgaatta gaatttaagg attatgcgga ttttgtaatt
    10741 aaaaatgaag gcaagacgat tgaaatgttt agtgtagatg catctgactt tcctttagtg
    10801 atacgtcgtg ttagagcaaa tgacacaatt caaatgcgat ttggaaataa gaatgtacac
    10861 cgattttttg tggatcgaaa aatcagtaag atatatagaa aatattggtt agttgtggaa
    10921 aataatgtgg gcaatgtgat ttttgtgcca ggacttggtt gtgatgtagg gcattattca
    10981 caaaatcaac agttctactt taaaataaat ggtcttgatt aaatgtttat gttacaatgt
    11041 gtaagtaact taaggagaaa aacaccatat gatagctcaa gatattgaaa aagttttatt
    11101 aagtgaagaa gaaattgaaa atcgttgtgt agaactagca catcaaatag aagcagatta
    11161 taaagaatct ggggatgttc caatcgtagt tggattatta aagggaagtg ttcctttcat
    11221 ggctgaattg atcaagcgtt ttgaatttcc atgtgaaatt gattttatgt cagttagttc
    11281 atacgatgga acagaatcta ttggagatgt tcgtatttta aaggatatgg acttatcttg
    11341 taaaaaccgt tctattttgg ttgtagaaga tattattgat acgggtagaa ctttggttga
    11401 agtaaaaaag atgtttaaaa ataaaggttg tctagatgtg cgttgtgtaa cattattaga
    11461 caaaccaagc cgtcgtgttt gtgaaattga ggcggattat gttggttttg aagtgccaaa
    11521 tgaatttgtg gttggatacg gattggatta caaccaaaag tatcgtaacc taccatttat
    11581 tggtgtttta aagccggaca tttatcagta ggaggaagat tatgaagaag tggttaaatt
    11641 atttaccgac catcttcatt ttatcaattg tattatcatt agttttttat agttcacagg
    11701 gcagtactgt acaaatgaat tatacacaat ttgagaaaat tgtagatgaa gttgatttta
    11761 aaaagagttc tatgacaatt agttccactg taatccattt ggaaggaact tatgagcaga
    11821 attcaaagac agttggatac aaggttattg ttccaagaac agaaaagaat attgaaaagt
    11881 tagaaaagtc tttacagaga aatggtggaa aattaactgt agaagatcct aatcaaggta
    11941 gtgtatggat ttcgattctt tctcaaatta ttccattctt aattatagct gtattctttt
    12001 atttcatgtt cgcaaaaatg ggtggtggag gcaacaataa agccttcgaa tttgcgaaat
    12061 caaaagctcg tgttgaatca aatgtaaaag ttcgttttaa agatgttgcg ggttgtgaag
    12121 aagaaaaaga agaagtaaaa gaaattattg attatttgcg ttcaccaaag aaatttacgg
    12181 atatgggagc acatattcct aaggggattt taatggtagg tcctccagga actggtaaga
    12241 cattacttgc gaaagctgta gctggagaag ccaatgttcc attcttctcg atttcaggtt
    12301 cggacttcgt tgaaatgttt gtcggaacag gtgcgagtcg tgttcgtgat atgtttaaaa
    12361 cagcacaaaa gagtgcacca tgtattattt ttattgatga aattgatgcc gtgggacgtc
    12421 agcgtggagc tggtatgggc ggtggaaatg atgagcgtga acagacgttg aaccaattgt
    12481 tggttgaaat ggatggtatg acagataatg cgggtattgt ggtaattgca gctacaaacc
    12541 gtcctgatgt actagatcca gcattattac gtagtggtcg ttttgatcgt cgtgttacgg
    12601 ttagtttgcc agatattaag ggccgtgaag ctattttaca tgtacatgct cgtaataaaa
    12661 aattggcgag tgatgtttca cttgcaaact tggcaaaacg tacaccagga ttttctgggg
    12721 ctgatttagc taacgtcttg aatgaaggtg caattttagc ggttcgtaaa aatgaagcta
    12781 aggtcactat gacagattta gacgaagcaa ttgaccgtgt tatgatggga cctgcgaaaa
    12841 agagtaagaa gtatacagaa aaggataaaa ttcttgtggc ttatcatgag gcaggacatg
    12901 ccgttattgg tttgaagctt gaagatgcag atatggttca gaaagttaca attattcctc
    12961 gtggagatgc gggcggatat aacttgatga cacctcgtga agagaaatac ttccacagaa
    13021 aatcagagtt tattgcacaa attactggtt tacttggtgg tcgtacagct gaggaaattc
    13081 aatttggaga aattagtgcg ggtgccgtaa atgatattga aaaattgact gaaatcgcta
    13141 agaacatggt tcgtgtatat ggtatgtcta gtttaggtcc tatccaatat gcggatcctc
    13201 aaggaaatgt cttcttagga agagattata caaagggtag tgattattct gcaggtgtag
    13261 cagctgaaat tgataaagag gttcgtgcaa ttgtggatga gtgtcatgaa aattgtcgta
    13321 aaatcttaac tgaaaacaaa gatttgttgg atttgattgc gaataatttg tatgaacatg
    13381 aaacattaac aaatgaagaa atcactaatt taatgaatta tggacaatta acagatccta
    13441 atgttgaaac aagtgaagaa gaggaaaaag cagaacaaga agtagttcaa gttccagaga
    13501 ttccggagac tccaactggt gttgatcaaa aggatttgga tgatgcgttc aatgaattaa
    13561 ctaagaaaaa agattaatta cacataaaag ccatcgattg atggctttta gcttggcttg
    13621 aaatggatac aagaattcag tagaatggtg ctagtgaatt ttattgtgga ggatattatg
    13681 aaagatcaac tattaaaagc tcttgtttta aatgagcatg ttcgtttata tattgtgcgc
    13741 acaactgatt tagtacagga agcgcaggat cgttttgatt tacatccatg tgcttgtgca
    13801 gctttaggaa gaacattgtc tgtcgcgagt atgatgggtg ctatgttgaa atcagaagaa
    13861 gaaatgttat caatcacaat taatggacat ggtccaattg gttcgattgt ggtagatgca
    13921 tatgcgaatg gaaatgttcg tgggtttgtt tcaaatccac atgtagagga tgttttgatt
    13981 cgtccgggta aactaaatgt tggggctgtt gtaggtacag atggaacttt aacggttaca
    14041 aaagatttaa gtatggaaga aaacttttct ggttcggttg agttacaaag cggtgaaatt
    14101 ggggatgatt ttgcctacta ctttacttta tctgaacaaa cgccaacagc ggtatcatgt
    14161 ggagtcttaa ttggtacaga tggtcgtgtt gccagtgctg gagctatgat tctacaaatg
    14221 cttccagatg caacagaaac agatatttct atttgtgaac acgtgcttga agggttgaaa
    14281 ccaatgagta ctttgattca ggaatatgat gattcaagtt tagaggcttt ggcaaatgat
    14341 atgtttgaag atgctaagat tttaacaaca caaccagtgg catatgattg tccttgttca
    14401 aaggtacgta tggctagagc tttgggtact attaaaaaat cagatgtgtt agaaatggcc
    14461 gataaagaac atggttgtga tatcacatgt aatttctgta atgaatcgta tcattttaca
    14521 gaagaagaat taagagcgat tgcgagtaag atgcatgatt aagattggaa atgtagaaat
    14581 tgaaaatcct gtagtcattg cgccgatggc aggtattagt aatatcgcat ttcgaagaat
    14641 tgcgaaacgt tttggtgccg gtttagtttg taatgaaatg gtaagtgaca aggcattgta
    14701 ctatgattca ttaaagacga aacaaatgtg tgccattgat gaaaatgagc atccagtatc
    14761 ttttcagttg tttggtcatg acattgatac ggttgtgtat gcagctaaat atttggatac
    14821 acaaacagat tgtgacatta ttgactttaa tatggggtgt cctgtaaata aggtcatcaa
    14881 agctcaggca ggaagttttt tgatgaagga tattgattat gccaaagagc ttgtatccaa
    14941 tattgttcaa aatgtaaata agccagtaac tgtaaagatg cgtttaggtt ttgatacgta
    15001 tcatatcaat tgtgttgaaa tggcgaaagc tatggagagt gtaggtgtta gtgctgtagc
    15061 tgtacatgga cgtacacgta atcaaatgta tgaaggaaaa gctgactggt cttatattaa
    15121 acaagtaaaa gatgctgtgt cgattcctgt tatcggaaat ggggatgttc gcagtgtagc
    15181 agattttcat cgtatgatga atgaaacagg gtgtgatgcc gttatgttag gtcgtggaat
    15241 tgttggaaat ccatttttga ttcaagaatg cgtggattcg attacaggac agaaacatga
    15301 attttcaatg gaagatcgca ttgagatttg tatggatcat gccagaggtt tagtaaatac
    15361 gaagggtgaa aaggtagcta ttcgtgaaat gcgaggcttg gctagttggt atttaaaggg
    15421 gcttccaagt tcaagaatct ttaagaatga gatgtcacaa atgaatacat tggaagattt
    15481 agagaatata ttacatcaat ttatggaggt gcagcgttag ttgcatcttt ttttgtatat
    15541 tggtaagcgt tttcaaaatc taaaaaatcc actttaagtt tctttcgttc cggtataaaa
    15601 tatatgtacg gaggttatat cgttatggat caaagaaaga caaaagcctt ttttgaaggg
    15661 cgtgatatac atgcttataa tatttttggt gcacatttag aaggggatgg cgttcgcttt
    15721 cgagtatatg ctccaaacgc aaaaaatatt tcagtcatag gtacattcaa taattgggac
    15781 gaccaagcta gtaaaatgaa aaaggataaa cgtggcattt gggaatgttt tgtgcaaggt
    15841 gcaaagaagc aggattctta taaatatcgt gtatggcagg caaatggtga attggtagat
    15901 aaaatggatc catatgcttt ttatagtgaa ttaagaccga acacggcaag tatcatatcg
    15961 gatttaagtt atgatggatg gactgacaaa gagtggttga atcaaagaaa taagggtttt
    16021 gattctcctg tgaatattta tgaaatgcat gttggttcgt ggaaaaaaga ggatgagaac
    16081 gaagaatttg tacaatatca tgaaatcgaa aaggatctaa ttgaacactg taagaaacat
    16141 cactttacac atgtagaagt tatgccgtta tgtgaatatc catttgatgg ttcttgggga
    16201 tatcaatgta cgggttattt ttcaagtaca tctcgttatg gaacaaatca tgaattgatg
    16261 cactttgtga atgctcttca tgaagctgga atcggtgtta tcatggactt tgttccagtg
    16321 cactttgtga aggataatta tgcattaggc atgtttgatg gcactccttt atatgaatat
    16381 tcaaaaccag aagatgccaa cagtgaatgg ggaacagcta actttgactt atggaaagaa
    16441 gaggttcgtt cattcttaat gtctgcagct aatttctgga tagacgttta tcatgtagat
    16501 ggattgagaa tggatgcgat cagtaatgcg attttctggc atggaaataa aaataatgga
    16561 gtaaatgaag gtgcatgtga ttttatgaag cgtatgaatc acttgttgaa tgaagaacat
    16621 caaggaaaaa tcatgttgat tgcagaagac tctagtgatt tccctaatgt gacgaaacct
    16681 agttatgcag gaggcttagg atttgactac aagtgggatc ttggatggat gaacgataca
    16741 ttgtcttatt tgaaaagaga tccaatttat cgtaagtggc atcaccacga tattacattc
    16801 tcaatggctt atttctattc tgaaagattt atcttgccat ttagtcatga tgaagttgta
    16861 catggtaagg caaccattgt agataagatg tggggaagct atgaagataa atttgcccag
    16921 gcaagagctt tatatgtata tatgtttgca caccctggta aaaaattgaa ttttatgggt
    16981 aacgaaattg gtcaattgcg tgagtgggat gaacaaaaga gttgtgactg gttcttattg
    17041 gattatccaa tgcatgatgc gtttaaacgc ttatgtgaag atttaggtaa aatctataca
    17101 gaaaatgatg cattcttcaa tcaggattat gatcctagtc gttttagctg gatcgatgcg
    17161 gataatgttg gacaaaatac attctcattc attcgtcgcg gttcaaagaa agattatgtc
    17221 atcattatga atttctctac aaatccatat gatcaccaac aatttggtgt tccttgtaaa
    17281 ggtaaatata aggaaatctt gaatacagag tgggataaat ataatggaaa tttaaaagac
    17341 catacgcctc aagaagtaca agctatgcgt ctagcaagac ataatcaacc atatatgatt
    17401 gaagtcaacg tacctagttt tggtgctatg atttatgaag ttgaaaagtg agattaattt
    17461 ctcacttttt ttgtgaattt ctcctttttt ttacgtataa gagagtgtgg aaaaaaggag
    17521 aacataaaat gaaaaaatta gatgtagaat ttgaattgtt tcgcaaaaga gtgtacaact
    17581 tgcaggtccg tgatttttat ttacttttaa tgaatcgagg atgtgatatg gatacattaa
    17641 gggtgttgtt ttcagatgta gatgataagc tgtccataaa attagatgga atgttctatt
    17701 atgcacaatc ggatatgatt caagaagtgt tagaaagagt aattaaacaa gaaaagacaa
    17761 atgcgagaat ggattgtttt aaaagactgt atgaaaaggg gatggatgtg tttgaggttt
    17821 tagaattgct tggaatttca caaaatgata tagaaatgat caatgaagca gcttatgttt
    17881 ggcaagatat gcatttgtga aataattgat ttttgaaaag ggtgtattgt gatgaaaagt
    17941 ttgttataat gtatgcgcaa aaaggagata acttaaatat tatggaaaac aaggaacttg
    18001 cattgcacgc aaaaaatatt cgtagaaata ttgtgaaaga agtgtatagt gcccaaagtg
    18061 gacacccggg tggaagttta ggtgcaactg acattttgac agagttatac tttgaagaaa
    18121 tggatattaa tgaggaaaac ttaaatacat taaatcgtga tcgttttgta ttgagtaagg
    18181 gacatgcaag tccggcttta tatgcagtac ttgctgaaaa aggattcctt ccagaagaag
    18241 aattgactac tttcagagct atcaattcac gtttacaagg acatcctaat atgaattatg
    18301 tagaaggcgt agatatgtct acaggttctt taggacaagg tatttcttgt gctgtaggta
    18361 tggcgttggc aaataaatta gataaaaatg atcatcgtat ttatactgta cttggagatg
    18421 gcgaatgcca agaaggacaa gtatgggaag ctacaatgtc agcagctcac tataagttag
    18481 ataacttatg tattattgtt gacaacaaca accttcaaat tgatggtcat gtagatgaag
    18541 ttatgagtgt ttatcctttg gataaaaaat tcgaagcttt caactgtcac gtaatcaatg
    18601 tagatggtca tgattttgat caattgcgtg ctgcactaaa agaggctcgt gaaacaaaag
    18661 gaatgccaac tgtaattgtt gccaagacaa ttaaggggaa aggtgtttca tttatggaag
    18721 atcaacaagg atggcatgga gtagcaccaa atgaagaaca ataccatgct gcaatgaaag
    18781 aattggaggc agaataatgg caaaagtagc tacacgtgtc gcttatggtg atgctttaaa
    18841 agaattaatg gtcgaaaatg aaaatgtcgt tgtattagat gctgacttag ctggtagtac
    18901 aaagagtgga gaagctaaaa aagtagatcc tgctcgccac tttgacatgg gaattgctga
    18961 aggaaatatg atgagtgttg cagctggtct tgcggctagt ggaaaaattg catatgcatc
    19021 aacttttgca atgtttgcga ctggacgtgg atttgaacaa atccgtaaca gtattggata
    19081 tcctcaatta aacgttaaaa tctgtgcttc tcacgcagga attagtgttg gagaagacgg
    19141 tgctagtcac caatgtattg aagatgttag cttaatgcgt ggtattccag gtatgaaagt
    19201 tatcgttcca tgtgactaca atgaagctaa acaagcatgc aaggctgtag ctgaaattga
    19261 tggtccttgc tacgttcgtt taggtcgtag tggcgttgaa agcgtaaatg gagacgacta
    19321 taaatttgaa cttggaaaag gtgttgtcct tcaaaaaggt gaaaaagtag ctctagttgc
    19381 gactggattg atggtacaag aagcattaga ggctgctaag aaaatggaaa cagctccaac
    19441 tgtagtaaat attcatacaa ttaaaccaat tgataaagaa ttgattgttg aacttgcaaa
    19501 aactcacgat aaaatcgtaa cttgcgaaga acattcaatt attggtggtt taggtagtgc
    19561 tgttgccgaa gtattagctg aagcaggaac agcatgcaaa cttgttcgtg tcggtgttca
    19621 agatgtattt ggtgaaagtg gaaaaccagc tcaattattc gcaaaataca agattgatgc
    19681 ggatgctatt atagaagctg ctacaaagta gtatggcaca ctatttcaca aatgatattg
    19741 taaaagatgc accagaagaa attacgatac attttcgtga ttttacatat aagctaaatt
    19801 cgaatgcggg tgttttcagt aaagataagc tagacgaagg aacacgcatc ctattagaaa
    19861 ctgtattaga caatcagtca gaaccagttt catgtttaga tctaggctgt ggaattggtc
    19921 ctattgcgct tattttaatg gaatattgga agcataccga aatgacaatg attgatgtga
    19981 atcaaagagc ttgtcagctt gctgattcaa acatgaaaaa gtatcgtcgt aaggcaaaga
    20041 taatatgtca gtctggtata aatgaaggcc agtatgcttg cattctttta aatccaccta
    20101 tacggacggg aaaggctatg atttattcat tgtttgatca atgtatggaa catttagcta
    20161 cagatggtca tttctggatc gtaatgcgca agcagcatgg tgctcaaagt gcaattcatt
    20221 atttacaaga aaaaggctat gaagtagaaa aaatggctcg tgaaaaaggc tattgggtca
    20281 tgaaaatatc aaaagcagga aattaatctt gcttttcttg tgcttttaca gataattcct
    20341 taaattcctt tggcgactac caaattcttt ttttaaggca cttaaaactt gtatagtctt
    20401 taaaaccata taatatacag atttctgtta aatttgtatg ataggatata gcgtctttgc
    20461 ataagcgaag acgtttttct tgtatattaa gattttggtt tggaaatagg gaaatgcagt
    20521 atgtgaaaaa ctatcaaatt gtcataattt gtgggttatc cacaacgaca aaaaggacga
    20581 tcgtaatagc caacattttg tatttgtctt tagttgttga attatttttt acaatcattt
    20641 tatgattaca ttcaggacag tgcatcatat agttttcttc aggttcattt ggaaataatt
    20701 catcgatcaa ccatcaaggt atttcttctt cataaccttt tcttatggac tttttgacaa
    20761 atgtcattta tcatcataaa tacagtagag gtattaatta atgaaacaac atcatgtgtt
    20821 tagtttttta caaaggatgg gaaaatcctt tatgttacct attgcagtgc ttcctgtagc
    20881 aggtattttt ttgggtgtag gaagttcttt aaccaatgaa acaacgatag ctgcattgca
    20941 tttagaattg gtattagggc aaggaacctt tttatatagt gttttaattt tattgaaaca
    21001 agtaggcaaa gttttatttg ataatctacc cttgattttt gcggtttcta tagctttagg
    21061 aatgtctaag cgaagaaaag aagtcgctgc tcttgcagct attattgcct tttttgtgat
    21121 gcatgccaca atcaatacgt tactttgctt ggatggtagt attgtcaatg gtgtagtttc
    21181 aaattatatt ctagatggat ctattacttc ggtatgtgga atgttatcgc ttgaaatggg
    21241 tgtgtttgga ggaattgtcg ttggacttgg tgttagttat ctgcataatc atttttacca
    21301 gattcaatta ccaaatgtga tttcttttta tgaaggagaa cgatttgttc caatcatttg
    21361 tgctattaca tatgtattta taggggtgct tatgtatttt atttggccac cattacagca
    21421 tggtgtttat aatttgggac aatttatatc tgactctggc tattttggta catttattta
    21481 tggaattatt aagcgtagtt tagttccttt tggattgcat catgtttggt atatgccatt
    21541 ttatcagagt gcattaggcg gtgtacaaat ggtaaatggt tcaatggtat ctggtgcaca
    21601 gaatattttc tttgcgcaat tatccgatcc aagtgtgact cattttagtg tggatgcaac
    21661 taaatttttc tctggagaat ttatttttat gatttttggc ttgcctggtg cggctttagc
    21721 tatgtatcaa tgtgcaaatc cagaagcaaa gaaaaagacg gcgagtttat tgctttcagc
    21781 cgctttaaca agtgcgttaa cgggaattac agagccgatt gaattctctt tcttgtttgt
    21841 ggcaccattg ttatatgttg ttcatgtgtt tttagctgct acatgttttg tggttgcaca
    21901 agctttacag gttgcgattg gatttacttt ttcagcaggg ttacttgatt ttacgttgtt
    21961 tggaatatta caaggaaatg cgaagacaaa ttggataact gtggttttgc ttggcttagt
    22021 atacttcttt gtgtattatg gggtatttaa gtttctgatt gtaaaatacg atttaaaaac
    22081 accgggaaga gacgaaagta ttaaggtgtt tgataagaaa aaatatgatt ttagctttga
    22141 taaatccttg attgacccaa gaagccagat gattgttcaa ggcttaggag gacgtagtaa
    22201 ctttacagac ttagattgtt gtatcacaag attaagagcg acgatcaaag atgattcttt
    22261 aattaatgag gggcttttaa aaaaatcggg ggctgcagct attatgtgcc agccaaatgc
    22321 gattcaaatt atttatggtc cacaagcttc aaatataaaa acaaaattgg atgagtatat
    22381 gttgaatgtg ccggaaagtt atgattttga ggataaagaa gttgttttgg aaacaaaaac
    22441 acagtttgaa cttgaatgtt tagtaaatgg ggaagtaatg ccaatagaag cggcatcgga
    22501 ttctatgttt gcgcataaat taatgggcga tggtattatg attcgtccat atgatggtgt
    22561 tgttgtagca ccttgtgatg gtgttatctc aatgatttat cctacaaagc atgcgattgg
    22621 tattacaatt gatgataata cacagatttt aattcatttt ggtacagatt cggcaaaatt
    22681 aaatggcaag ggctttgaat tgttggttag accaaatcaa gaagtaaaag ctggagatgt
    22741 tttgtggaat gcggatttaa agtatatcaa agacaatgcg ttagatgaaa atattatagt
    22801 tgtatttacg aaaataaaag atggtgcaga actagaaaag aaatatggaa aaatgcaatg
    22861 tcatgatatg atgttaaggg taaagggata atggatatgt gtgaaattgt gaagattttg
    22921 aatcataata caatcttggt tttaaaagat aatgaagaaa taattgtgat gtctaaagga
    22981 attggatttg gtaaaaaaca aggggaacag gtggatgttc ctcgtaatgc aaagcagtat
    23041 aagatgcaaa agtcatatca ggcgaaacag aagtcgaata atatttttga ttacattgat
    23101 ccaatgtata ttgaaatttc atctgaaatt attaccttaa caaaagaaag atttggtaaa
    23161 gtagagcacg atattttatt atctttagcg gatcatattt attttgcggt aaaaagaatt
    23221 aaagagaagg attttccatc aaatccattt aatatggata ttcaacttat gtttccggat
    23281 gagtattcag tggcattaaa ggcaaaggat attattgaaa aatatacgca tgaggaaata
    23341 aatgcagatg aaattgcgtt tatcacctta catattcact cggctatatc ggtaaataaa
    23401 gtagggcagt ctatggaagt tatgcgtgtg attcgtgaat tcttcaagaa attacaggcc
    23461 gatttaaata ttcgtattga tgcgaattca ttgtcatata ttcgtttaat gaatcatatt
    23521 aagtttttgt tgttgagact aaataataat gaggaattac aaatggatat tacagaattc
    23581 acaaaagaaa agtttccatt cgcatatgag caggctcgtg ttttatgtga acaattgagt
    23641 catgttttac gtaaagattt gccggattca gagttgggtt atttggcttt acacttggaa
    23701 agaattttat cgagtacgag tatttcataa aaaaatgttg acaacgcttt cagaaagtga
    23761 tacatttttt catgtaaaaa gtgaatattc aatagcgtgt aactggttag gcaggcattt
    23821 actattttaa ttgaagatat aatctttatt ggtttattgt atatcttcag tttaaatgta
    23881 tatgtctgtc tttttttgcg tattgaattt caaagaaagg atgaaaatat atggtaggaa
    23941 ttattcttgc aagtcatggt gaatttgcga atggtatctt ccagtcagga gctatgattt
    24001 ttggtgaaca agccaatgta aagccttgta ctttgatgcc tagtgaagga cctgaagaca
    24061 ttcgaaagaa gatggaagat gcgatcgctt cttttgaaaa tcaagaagaa gtattgttct
    24121 tagttgacct atggggagga actccattca atcaggcaag cgccttgtta gatggtcacg
    24181 aagacaaatg ggcaatcgtc acaggattga atttgcctat gttgattgaa acgtatgctt
    24241 ctagattctc aatggagaaa gcacacgaaa ttgcagcaca cgtaatgcag actgcacaag
    24301 agggtgtaca gatcaatcca aaagaattag aaccaaaaga agaaaagaaa gtagaagtaa
    24361 gtgcagcacc tcaaggagca attcctgaag gtacagtttt aggagatgga catatcaaat
    24421 atggcttagc acgtattgat acacgtttac ttcacggtca ggtagcaaca gcttggacaa
    24481 agacagttgg accaaaccgt atcattgttg ttagtgacaa tgtagctcat gacgatttaa
    24541 gaaagagcat gatcgaacag gcagctcctc caggagtaaa agcaaatgtc gttccaattg
    24601 ataagatgat tcaagtcgcg aaagatcctc gttttggagc cacaaaagca atgttgttgt
    24661 ttgaaacacc acaagatgca ttaagagcta tcaaaggtgg agttgatatt aaagaattga
    24721 acttaggttc aatggcacac tcacaaggaa aggttgtatg tacaaaggcc gtttctatgg
    24781 gtagtgatga tgtaaaaaca tttgatgaat tattagcaat gggaattaag atcgatgttc
    24841 gtaaggtgcc aacagattca cctgaaaact tcgatgaaat tcttaaaaaa gcaaaaacag
    24901 aattaaagta aatttagagg gagattatta tgtcagcgat tagcattatt ttagttataa
    24961 ttgttgcctt cttagccggt atggaaggaa ttcttgacca atttgaattc caccaaccat
    25021 tagtagcttg tacattaatt ggtttggtta ctggtcactt aaaagaaggt gttatgttag
    25081 gtggttcttt gcagttgatg gctttaggtt gggcaaacat tggtgcagct gttgcacccg
    25141 atgctgcatt agcttcagtt gcttctgcaa ttatcatggt tttaggttta aatggtggaa
    25201 caacagatac tacaacggct atttctacag caattgccgt agctattcca ttatcagtag
    25261 ctggtttatt cttaactatg atttgccgta caattgcaat tccaattgtt cacttcatgg
    25321 atgctgctgc tgaaaaaggt gattttggag caattgaacg ttggcaaatt gtaggtattt
    25381 gtttacaagg tgttcgtatt gcgattcctg ctgctgcatt atgtttcgta cctagttcag
    25441 ttgtaactgg tgtattaaat gcaatgccag cttggttatc tggtggtatg acagttggtg
    25501 gaggaatggt tgctgctgtt ggttatgcaa tggttatcaa catgatggct acaaaagaaa
    25561 cttggccatt cttcgcttta ggtttcgtat ttgcttgtgt aagtgaattc acactaattg
    25621 ctttaggtgt aattggtgtt gttatcgcaa tcgtttattt aggtttgaaa gatgctgcta
    25681 aaaatagtgg tggaggatca aatactggtt ctggtgatcc attaggcgac atcttgaatg
    25741 attactagga ttttgaagga ggtaaatgaa aatggctgaa aaaattacat taactaaaca
    25801 agatcgtaaa aaagtttggt ggcgtcatca attccttcag ggttcttgga actatgaacg
    25861 tatgcaaaat ggtggatggt gctattcaat tattcctgca atcaaaaaat tatattctaa
    25921 caaggaagat caaattgctg ctttaaaacg tcacatggag ttctataaca ctcaccctta
    25981 tgtatcaagt ccagtaatgg gtgttacact tgctttggaa gaagaaaaag caaatggtga
    26041 aaatgtagat gatactgcaa tccaaggtgt taaagtaggt atgatggggc ctttagctgg
    26101 tgttggtgac ccagtattct ggtttacaat gcgtccaatt ttaggtgctt taggagcttc
    26161 tttagcatta agtggaaacc ttttaggacc tatcttattc ttcgtattat ggaacgtaat
    26221 tcgtttagca ttcatgtggt atacacaaga atttggttac aatgtaggta ctaaaattgc
    26281 taaagatatg tcaggtggac ttcttggaaa aatcactcaa ggtgcaagta tcttaggtat
    26341 gtttatcatt ggtgcattgg tacaacgttg ggtaagtatt tcatttactc cagttgtatc
    26401 aagtgttgct caatctgaag gtgcatacat tgattggtca caaattaaag gaaatgctga
    26461 cggaattaag agtgcattag aacaatattc ttcattagga gctactggtt taaatcaaat
    26521 caaagttaca actttacaac aaaacttaga ttcattgatt cctggtcttg cagctttatt
    26581 attaactttg ttatgctgta aattattgaa gaagaaagtt tctccaatca caattatcat
    26641 cgcgttattc gtagttggta ttattggtcg tgtaatcgga attatgtaat agcttaagga
    26701 ggtacatatg gcgcaatcat taaattcaaa agttgattta acaatcaagg caacatcttt
    26761 tgcgggattg agttccaatg gaaatgtcat ggttggaaa
//
LOCUS       DBMDWL010000007        25482 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_728_length_25482_cov_2.984792, whole genome shotgun sequence.
ACCESSION   DBMDWL010000007 DBMDWL010000000
VERSION     DBMDWL010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 25482)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25482)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25482
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_728_length_25482_cov_2.984792"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="ACZPDN_00930"
     CDS             complement(<1..307)
                     /locus_tag="ACZPDN_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014177.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HIR1297222.1"
                     /translation="MFYSPLRYPGGKGKLEPFMELLIKQTGHQGGVYVEPFAGGAGIA
                     LELLEKEVVNDIVINDLDKGIYSFWKAILTETDRFITDIRNVELTMDEWNRQRQVIDN
                     "
     gene            complement(324..1244)
                     /locus_tag="ACZPDN_00935"
     CDS             complement(324..1244)
                     /locus_tag="ACZPDN_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018339.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HIR1297223.1"
                     /translation="MDKKIEETREFLQTIGMPKAQLADICCYVILAMAGIKPDMSWSE
                     ATNEWIRIHDIIQFVNTFYGMSYAENSRETFRKQALHRFRTAALIEDNGKATNSPNYR
                     YRLTEETIKILRTMETPAWKESIKRFLCYHEKLIDLYASKKKMTMMPVNINGESFKFS
                     AGKHNELQKAIIEEFAPRFAPNSECLYVGDTIEKDLVKNVEKLKELGFEITLHDKMPD
                     VVLYREDKNWIYFVESVTSVGPMDPKRILEITEMTKDVTAGKIFVTAFLDFKTYKKFA
                     EELAWETEVWIAEMPEHMIHLNGDRFMGPR"
     gene            complement(1237..2724)
                     /locus_tag="ACZPDN_00940"
     CDS             complement(1237..2724)
                     /locus_tag="ACZPDN_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HIR1297224.1"
                     /translation="MLEKIIELTNEYIESMPKKERKKYGQFFTSMETARFMSGLYNFD
                     EKTGKVSVLDAGAGSGILSCAFIERVETIDSIQEIELTCYENDENVLPLLKRNLEYCK
                     EETKKKITVNIIEDNYILSQYLDFNHMLGGNAEPKKYDFVIGNPPYMKIPKDAPEATA
                     MPEVCYGAPNLYFIFASMGLFNLYENGEMVYIIPRSWTSGAYFKRFREYFLTEGKLKH
                     IHLFVSRNKVFDKESVLQETIIIKVKKTSEKPETVTITSSKSNSDFGELTSLTVPYDL
                     VVAGSDYYVYLVTDENEVEVLKKLHKFDKTLPAIGVKMKTGLTVDFRNRDILRDEAEE
                     GAKPLFYSQHIKQGKVEFPIQKEHEYVVTEQKGLMQDNKNYLFVKRFTAKEEPRRLQC
                     GVYLAKRFPQYQKISTQNKINFVEGVLTEMSECLVYGLYVLFNSTLYDEYYRILNGST
                     QVNSTEINAMPVPELEDIQEMGRKVLKSKDYSEANCNLILEGYCG"
     gene            complement(2807..3583)
                     /locus_tag="ACZPDN_00945"
     CDS             complement(2807..3583)
                     /locus_tag="ACZPDN_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HIR1297225.1"
                     /translation="MSALDIIHELIKNVKIGIIRNMEKKKMTQEDIMKLLDSCYEKCL
                     NGIPMVSPGVEDMANDYLSKHETKEKACRDMLKNQIAKCTTSGVVTGLGGFITMPVAI
                     PANIGSVIYVQMRMIACTAYMADYDLNSDQTQTFVYACLAGVAVNSLLKQAGIKFGVK
                     FANGVIKKIPGKVLTKINQKVGFRFITKFGSKGIVNLGKMLPGVGAIVGGGLDLVETK
                     VIADRAYKWFMTGDFSVQGESNDDVIDIDDNDYNTTEIKE"
     gene            complement(3691..4068)
                     /locus_tag="ACZPDN_00950"
     CDS             complement(3691..4068)
                     /locus_tag="ACZPDN_00950"
                     /inference="COORDINATES: protein motif:HMM:NF025561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIR1297226.1"
                     /translation="MEKHIMGENGISYTLGEDGLYYPDLYLPEETEYSIGKYGMLRKA
                     YLKEHRKGLYLELVLAGKLNEHLHQIDEECNQMMDKLMEQMKEKQGVTEELKMQDQME
                     WVGRMNNIRACAEEIVLKKIVYR"
     gene            complement(4079..5605)
                     /locus_tag="ACZPDN_00955"
     CDS             complement(4079..5605)
                     /locus_tag="ACZPDN_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR1297227.1"
                     /translation="MPIGRNSFIQMTKLSNVKGRITYISSHAKQENLYAVYETTERKF
                     WRELAKCNQEEFAKSGTEGKCIEARELIIALPESFTEYQPDRLLQLFTNHFKQNYGTE
                     CIAALHHNKRKTNYHIHLIFAERKLLDEPIIKIASRNMFYDENGKHVRTKKEILGEDG
                     EIREGCSIVKKGEVHEKKLFTAKDERFKCNSFLDEVKHSYTDLINIYVQDDKQKLQVF
                     ERGSVYLATKKIGKNNPKAQEIEADNQKRREWNRAVDMALISGVPEPKIMEVKRKEIT
                     EPIRESIARRGNRPRLFSRVVTVAVEALEFLIASVLFKKPREVPKQIEQTETEHPKHV
                     AENSTATVKTTKEEKAEPEQPKMTRLASKYPKLFKVNKELEDQNGAIQQKQKQLSAKK
                     KELSEVKGWFKGRKKKELQKEIVELKSQIRDMKDYLPRLVQKIGYRSVQEFLKDFKDS
                     QTEYSQYRTAIEKWKKETGKEPVAHGIRAKLAEKKQEIQNEQKNKQHTRSQNKDRGVR
                     "
     gene            complement(5920..6147)
                     /locus_tag="ACZPDN_00960"
     CDS             complement(5920..6147)
                     /locus_tag="ACZPDN_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HIR1297228.1"
                     /translation="MREGRLGYNSYNKRYGLLSSDLWIDTGFHCGECLEVLVDDQWVK
                     TRMEMNLAREWYLVGTPYCGDLEYVRARIPE"
     gene            complement(6322..6564)
                     /locus_tag="ACZPDN_00965"
     CDS             complement(6322..6564)
                     /locus_tag="ACZPDN_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297229.1"
                     /translation="MERKLQVQIPKDLLLALFQYHLARNEEYLPEIEKALMEKLDSMV
                     KRQLYTTFKTAPTEEEREKARQEYLDKCGMHENFRW"
     gene            complement(6656..7810)
                     /locus_tag="ACZPDN_00970"
     CDS             complement(6656..7810)
                     /locus_tag="ACZPDN_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1297230.1"
                     /translation="MTEKEKMTAPIASVGADVEQSSSNKLTNQSIADLPGKGNLQATN
                     NVENTAKSANKKNPDDLETVSMMELYDTAYPPKLPIIDGLLYNGTYLFVGSPKIGKSF
                     FMAQIGYHISKGIPLWGFSVRQGTVLYLALEDDYARLQKRLSQMFGVEGSENFYFATK
                     SKSLNDGLEKQLVTFVTEHKDARLIIIDTLQKVREVGGDKFSYASDYEIVTKLKAFSD
                     KYGICFLVVHHTRKMESSDSFDMISGTNGLLGAADGAFVMQKEKRTDNKAVLEVAGRD
                     QQDQKLWLNFNRERCVWELTKTETELWKEPEDPLLEKIKELVTIDQPEWTGTATELVQ
                     ILQAEMQPNALSRKLNVSLERLILEYGIQYKTERGHDGRKIRLTFVGKQA"
     gene            complement(7807..8130)
                     /locus_tag="ACZPDN_00975"
     CDS             complement(7807..8130)
                     /locus_tag="ACZPDN_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIR1297231.1"
                     /translation="MEKNLDSKGRWRNKIVAFRVSPEEAEQIDACVRLSGLSKQDYIT
                     KRLTDREIVVQGNPRVYKALRNQMAEIYEELKRLEKCSEENEELLSTLRLVAETLYGL
                     KGENE"
     gene            complement(8108..9208)
                     /locus_tag="ACZPDN_00980"
     CDS             complement(8108..9208)
                     /locus_tag="ACZPDN_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR1297232.1"
                     /translation="MSIYKDNVTGKWRVVYRYTDWTGKTKQTSKRGFPTKREAQMWEH
                     EQMLKHDAKLDMTFASFYEIYVEDKKERIRDNTWGTKNNIARTKILPYFGERKIAEIE
                     PKDVIAWQNHLLAFKRANGKGYSASYLRKIHSQLSAIFNHAVDFYHLPSNPAQKAGNI
                     AIEEYKEMLFWTKEEYLKFADVMMDKPVSYYAFEMLYWCGIREGELLALTPADFDFTT
                     HTLRINKSYQRLNREDVITPPKTFKSNRFIKMPQFICDEMQDYLKMLYEPKEDERIFT
                     ISKSYLHHEMNKGSKVSGVKRIRVHDLRHSHVSLLINMGFTVLAIADRMGHESIDITY
                     RYAHLFPSEQTQMAEQLDIERMEKSNGEESGQ"
     gene            complement(9226..9462)
                     /locus_tag="ACZPDN_00985"
     CDS             complement(9226..9462)
                     /locus_tag="ACZPDN_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297233.1"
                     /translation="MQMQQPKQNALRRKEGCDVTNRTFMTVEEVAAELGVSKSYAYKI
                     VKQLNEELQKLGYLTVAGRVNTNYFRKKVCYSEV"
     gene            9641..10165
                     /locus_tag="ACZPDN_00990"
     CDS             9641..10165
                     /locus_tag="ACZPDN_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297234.1"
                     /translation="MAIGQRIKYFRNRKGMTQKQLGEQLGFMGKTSDVRMAQYESEAR
                     VPKIDLVKQMSQIFGVNTHALTVPDIDTHIGLMHTLFALEDMYGLKVKNVDGQPHLCL
                     DSSISAPGSSVDEMLRAWMDQTDKLENGEISKEEYDKWRYKYPELDTYQKRAKVPSQE
                     LSDYLVKELTKKEK"
     gene            complement(10357..11904)
                     /gene="guaA"
                     /locus_tag="ACZPDN_00995"
     CDS             complement(10357..11904)
                     /gene="guaA"
                     /locus_tag="ACZPDN_00995"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1297235.1"
                     /translation="MKKELVIVLDFGGQYNQLVARRVRECNVYCEIYSYKTDLEKIKA
                     MNPKGIILTGGPNSCYEADSPTYSKELFELGIPVLGLCYGAQLMMHVLGGKVEKADHR
                     EYGKTEVLVDKTSSKIFEGVSEKTICWMSHFDYISQVAPGFEITSHTDNCPCASSENA
                     EKGLYAIQFHPEVLHTQEGSKMLYNFVRGVCGCCGDWRMDNFVEEQIKAIREKVGDGK
                     VLCALSGGVDSSVAAVLLSKAIGNQLTCVFVDHGLLRKNEGDEVEAVFGPEGPYELNF
                     IRVNAQQRYYEKLKGVEEPEAKRKIIGEEFIRVFEEEAKKIGKVDFLVQGTIYPDVVE
                     SGLGGESAVIKSHHNVGGLPDHVDFKEIIEPLRDLFKDEVRKAGLELGIPEYLVFRQP
                     FPGPGLGIRIIGEVTEEKVKIVQDADAIYREEIANAGLDCSIGQYFAALTNMRSVGVM
                     GDERTYDYAIALRAVNTIDFMTAESAQIPYEVLNKVMSRIINEVRGVNRVMYDLTSKP
                     PGTIEFE"
     gene            complement(11952..13451)
                     /locus_tag="ACZPDN_01000"
     CDS             complement(11952..13451)
                     /locus_tag="ACZPDN_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR1297236.1"
                     /translation="MRKIGWIGAGIMGQPMVIHLLNKGYEVHVYARHPERVEQAKNAG
                     AILEESIVSLASNVDVICTMVGFPSDVKEVYDVIFSCIETGKTCIDFTTSSPKLAKEL
                     YTKGKELGVHVLDAPVTGGDTGAKNGTLTVLVGADKEDFETNKEIFEAFGTQIEYCGK
                     ASCGQHVKMANQIMIANTLQGICEAMSYLKCKDVDESFVYRFLRNGAAGSKQLEFQGK
                     KMLENDYAPGFYVKHFVKDLKIAVQEANFPLYGVNRVIKEYVDLMDRGMSDLGTQCLI
                     EYFRKPQIKAVIFDMDGLMFNTETMFKDEFKEKAKELGVSCPDYFPEPLIGCDSRKVA
                     EFEMMYPGVTRVMEEIQKERADYFFTYFKEPGSANMVGLQNLIEYIEENKIPYAVASS
                     SHPQDIKKFLSHAGFVLSPNVIVSSKEGYKSKPAPDVFLAAAKRLGMEPENCLVLEDS
                     KHGIMAAANAKMHSIFIQDQIAPDDEMKEYIQESCTDLNEVIDYLKRCK"
     gene            complement(13453..14574)
                     /locus_tag="ACZPDN_01005"
     CDS             complement(13453..14574)
                     /locus_tag="ACZPDN_01005"
                     /inference="COORDINATES: protein motif:HMM:NF022635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mbeg1-like protein"
                     /protein_id="HIR1297237.1"
                     /translation="MENLLNYVHLRRDIDFSVRPFNEVDMLVCADLSYVDWDGIVEKD
                     EVSLEEACRKYLSLYSEEELKSKYTFSMNLPHLIDALQDTIRYGNIKLKNYKKVYSDE
                     DVIQFSVVTLVLPDGSFVISFSGTDGSITGWKENLMMTYSKDLPCQILAKDYVKETIA
                     DIPETSYLFGLKKKKVYPKMYLTGHSKGGNLAMYAYLKNPELQGYIEGVKSFDGPGFA
                     DGFWQGDEDVSKITNYIPKDSIVGRVLDHREQTKVMDAEGSGLVQHDTLMWSVDVKDF
                     NYCDALTKESDDLLEYVNKLLMNRPLEEKERYCHLIGELFDRMEIYTIADLTEFSFKQ
                     ALSGIKEIRQLNAEEIKFMFEVVKFIAVQSAPILVKGRK"
     gene            complement(14627..15583)
                     /locus_tag="ACZPDN_01010"
     CDS             complement(14627..15583)
                     /locus_tag="ACZPDN_01010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1297238.1"
                     /translation="MQELRLLQEKDLESIYPIYVHYVKTSVAIFDVVPDPFDVFKEHM
                     MEVSKTNPFYVALNDDVLIGYGYVHPAFSKEAYKYCVELTIYFKEGKHYGLPSKMLDQ
                     LEADCRKLNMRWIISCITDSNEESIAFHKKHGFTMYGALPSCGMKFDVWHGVVWLCKR
                     LDEVKKDFSCASNATILGNVSIGKGSSVWYNAVIRSEEETIEIGQESNIQDQCVLHTD
                     CGYPLKIGNRVTIGHGAIVHGCTIEDEVLIGMGAIILNGACIGSHSIIGAGCVVPENM
                     VIPQKSVVVGVPAKIIKKISESQVSDILSNADHYIKLSKKLG"
     gene            complement(15592..16593)
                     /gene="tsaD"
                     /locus_tag="ACZPDN_01015"
     CDS             complement(15592..16593)
                     /gene="tsaD"
                     /locus_tag="ACZPDN_01015"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1297239.1"
                     /translation="MIIIGIESSCDETAVAVVKDKKEVLSSVVASQIDVHTEFGGVVP
                     EVASRIHVENISYCIEKALKDANITMEDVDAVAVTQGPGLIGCLHVGVQAAKTLAFAY
                     HKPLVPVHHLAGHIYANELVVDMKYPVLALVVSGGNTELVYMKDETSFEILGETQDDA
                     IGEAFDKVARVLGLGYPGGPKIDKLAKEGKPVYELAKPKTQGRYDFSFSGLKSSVLQF
                     TKRMERQGKTFDMADLACSFQECALDEIFSRVRAVLDDHKDIRHFVVGGGVSANSRLR
                     EKVEELRNEYPEVEFTVPPMYCCTDNASMIGVAGTIAYLSGRRADASLTADSSLEIQ"
     gene            complement(16603..17037)
                     /gene="rimI"
                     /locus_tag="ACZPDN_01020"
     CDS             complement(16603..17037)
                     /gene="rimI"
                     /locus_tag="ACZPDN_01020"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR1297240.1"
                     /translation="MIFKEMKDTDLVRVLELEQACFKQPWLEKDCLYELNENPFSHGW
                     ILLDEGIIVGYAFLWETFEMAQLARIGVDPDLRKRGYGKELMMHLFDRAREVACEYMT
                     LEVRASNEAALNLYQKMGFIQVNVSKSYYPDGEDAIVMTLAL"
     gene            complement(17034..17627)
                     /gene="tsaB"
                     /locus_tag="ACZPDN_01025"
     CDS             complement(17034..17627)
                     /gene="tsaB"
                     /locus_tag="ACZPDN_01025"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR1297241.1"
                     /translation="MISLCMDSAYKALVLGLYKDGTLIDGVSIEAFKKQSETIFVELN
                     QLFEKTGLDYKDVDEVIITDGPGSYTGIRIAMTIAKVFCTQMHKTLKCISTMQLFAGM
                     DESANVILDARSKRAYVAHLEKGVVVGKTQILEVDQLEDFLNEHPGTLYGDGYLVNQE
                     AASCDFLKNFMEVPSRTIENVHALVPQYLKESDAYKA"
     gene            complement(17620..18072)
                     /gene="tsaE"
                     /locus_tag="ACZPDN_01030"
     CDS             complement(17620..18072)
                     /gene="tsaE"
                     /locus_tag="ACZPDN_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1297242.1"
                     /translation="MKIEIHSLQETQEFATKMADLCRNKQLVITLDGDLGAGKTTWTK
                     SFGKALGVKNVINSPTFTILKDYKQEGGVPFHHIDAYRLEGKCQDLGFEDCFDEGITV
                     VEWSEFIEDQLPQDHIKISFEEGIDENRTVTLEYTGPVSKSIVEAYHD"
     gene            complement(18124..19467)
                     /locus_tag="ACZPDN_01035"
     CDS             complement(18124..19467)
                     /locus_tag="ACZPDN_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1297243.1"
                     /translation="MEQDSLFSIQEDTRPLADRMRPETLDEYVGQKQLVAKGNLLWQM
                     IEHDQVTSMIFWGPPGVGKTTLARIIAHQTKSYFVDFSAVTSGIKEIKEVMKQADTCR
                     ALGQKTILFVDEIHRFNKAQQDAFLPYVEKGSIILIGATTENPSFEINSALLSRCRVF
                     VLNSLNTDDLKDLIIRAISSSKGLGNLKIHITDEDIEAIAAFSGGDARFCLNTLEMVV
                     YNSPSELDGIHVSQDILKQCLQKRSLLYDKKGEEHYNIISALHKSVRNSDVQAAVYWL
                     ARMLEAGEDPLYIARRFVRMASEDIGMADSRALEICVAAYQACHYLGVPECNVHLTHA
                     AVYLALAPKSNALEVAYNNAKSDALKTLDQPVPLHIRNAPTKLMKDLGYSKGYEYSHD
                     YEYHMTDMQCLPDALVGKEYYVPSDQGSEVKVRNRLAQIKQIKAMVHRNRMMKKK"
     gene            complement(19524..20876)
                     /locus_tag="ACZPDN_01040"
     CDS             complement(19524..20876)
                     /locus_tag="ACZPDN_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297244.1"
                     /translation="MEENNKMKNEPVNKLMLQMGVPMILSMALQAVYNIVDSAFVGNM
                     KEGSEAALNALTLVFPIQMLMVALAIGTGVGVNALLARTLGQRNREKASKVAGNGLFL
                     ATVIYIICVLFGLFGIKAYISSQTRNALVLSMSVDYLRICCLLSFGVVFFSIFEKLLQ
                     ATGRSLYSTIGQVTGAVINMILDPVLIYGFNLGVQGAAYATVIGQILSALLLVVFHMR
                     LNKEFDHGLTYTKPNKRIINEIYSIGLPAIIAQALMSFMVYAMNLILKFDASAQTAYG
                     LFYKVQQFVLFMAFGLRDAITPIIAFSYGMQNKQRVKDGIKYGLIYTIVLMVLGTLIT
                     ELFPNFFATLFNAGQSRIYFISAVRIISVSFVFAGVNIAFQGVYQALNGGMESLIVSL
                     LRQAILILPLAWGFSKLVIQYDRDVSLIWYAFVITEVLSCVVGWVFLRRIEKKKMVFD
                     "
     gene            complement(21051..21617)
                     /locus_tag="ACZPDN_01045"
     CDS             complement(21051..21617)
                     /locus_tag="ACZPDN_01045"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1297245.1"
                     /translation="MIITITREFGSGGRTIAKKLAEKLGYAYYDYEIVQQIAQESGFA
                     KEYIEENGEDASSTSTFGFIWNNYGYNLSDELYVAQRNVICELADKGNCVIVGRCSDY
                     ILRDRKDVLNVFIHADFEYRKNRVVSVYGQNEFAPDKRIKDKDKRRIAYYKYYTDRKW
                     ADAKNYQLCLDSGVLGIDKCVELIEKAM"
     gene            complement(21971..22630)
                     /gene="pyrE"
                     /locus_tag="ACZPDN_01050"
     CDS             complement(21971..22630)
                     /gene="pyrE"
                     /locus_tag="ACZPDN_01050"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1297246.1"
                     /translation="MEQYKKEFIDFMLESKVLKFGDFTLKSGRKSPFFMNAGGYVTGN
                     QLKRLGEYYAKAIVNQYGKDFDVLFGPAYKGIPLAVATTIALDNLYGMEVRYCSDRKE
                     EKDHGADKGSFLGTKLQDGDRVVMIEDVTTSGKSMEETVPKVRGAANVEIVGLMVSLN
                     RNEKGKGEKTALTEVSELYGFPTAAIVSMPEVIEYLGDKLDPELKSRIDAYYEEYGAK
                     E"
     gene            22743..23159
                     /locus_tag="ACZPDN_01055"
     CDS             22743..23159
                     /locus_tag="ACZPDN_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1297247.1"
                     /translation="MQENVFFFLKAKNSVKYLYDNISVEDGLALMRKHKYTAMPIINK
                     EGVYVGTITEGDFLWYLLDHPSIHKEDLEQMEIHELIRGDFTPPVQIDVPVDQLFAQS
                     LKQNFVPVVDDRQIFIGIVTRQSIIQYLMNNASKYL"
     gene            23310..24500
                     /locus_tag="ACZPDN_01060"
     CDS             23310..24500
                     /locus_tag="ACZPDN_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1297248.1"
                     /translation="MNLPILILFTFILNILFSMTSSIVSTNKEIEDESYKYQATLKWL
                     SLCMEVFGTLMSVIYLQSLIHGPLLYVVVLLLVYVYILLSDLLPRKFANAHLDKFEKT
                     FMSIAKGIQSLFTPFTFFLRFEVEKEQEDYSEEDIYEVINGGGVEPSQKEFIENLFEF
                     DDTPVEEICTHRSEVVCLYLNDDKETWKKTILENRHTLYPVCDEDNDDVVGILDTRDY
                     FRLDSIEQDNVINKAMDQPFFISQNMKADVLLKEMKIKKVYFAVLLDEYGGMTGIVTL
                     HDIIETLLGEIQEDDDIEEPDPIQQIDADQFRIYGQADIEDVEKALGISLEDEDCDTF
                     GGYILNHYGQIPDEGSHFKVSLDVMDVYVKEVKNHRIGQTIVQIKRKEEGNQHESTEK
                     RNRD"
     gene            24469..25041
                     /locus_tag="ACZPDN_01065"
     CDS             24469..25041
                     /locus_tag="ACZPDN_01065"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR1297249.1"
                     /translation="MKALKNAIETRGHFINDEVLKVDAFLNHQIDCSLVDEMGNEFYE
                     HFKDKGITKVVTIESSGIAPAYATAIRLNVPLIFIKKTQPSTMIDPAFAEVFSFTKNK
                     TYPICIEKEYLKKGDKVLFIDDFLANGEAFKGTENLIAQAQAEIVGVGMVIEKAFQKG
                     HAYIVEKGYDLLALASIAGVKNKKITWADE"
     gene            25076..>25482
                     /locus_tag="ACZPDN_01070"
     CDS             25076..>25482
                     /locus_tag="ACZPDN_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297250.1"
                     /translation="MKNTFKKLFISFTVLSLTACSSTTMQDNTFVAKENKDFTKYTNQ
                     LVVEYAENDYTTMHQFFEHPEDYGIDINNVDVSLGEFYTDNTEEIKEDQKELNKFNRN
                     TLDKTQQTIYDELKYENELSLKAENKKFQYLNN"
BASE COUNT         7624 a         5659 c         3623 g         8576 t
ORIGIN      
        1 aattatcaat tacctgacgt tgtctgttcc attcatccat agttaattct acatttcgta
       61 tatcagttat aaatctatct gtttctgtaa gaattgcctt ccaaaatgaa taaatcccct
      121 tgtctaaatc attaataaca atgtcattta caacttcttt ttccaataat tctaacgcaa
      181 ttcctgcacc accagcaaaa ggttctacat aaactcctcc ctgatgcccc gtttgcttaa
      241 tcaataattc cataaacggt tcaagttttc cctttccacc aggatatctc aatggagaat
      301 aaaacatgtc acacctcatc ctttcatctc ggtcccataa atcgatcacc attcaaatgt
      361 atcatatgct ccggcatttc agcaatccaa acttccgtct cccaagccaa ctcttccgca
      421 aatttcttat acgtcttgaa atccagaaat gctgttacaa atatttttcc tgctgttaca
      481 tctttcgtca tttctgtaat ttccaatata cgttttggat ccatcggacc gaccgatgtt
      541 accgattcca caaaatagat ccaattctta tcctcacgat atagcactac atccggcatt
      601 ttgtcatgta acgtaatttc aaatcccagt tcttttaatt tttctacatt ctttaccaaa
      661 tctttttcta ttgtatcgcc aacgtacaag cactccgaat ttggtgcaaa tctcggtgca
      721 aattcctcga taatggcttt ttgcagctca ttatgcttac ctgctgaaaa tttaaagctt
      781 tcaccattta tatttactgg catcattgtc atcttctttt tagaagcata caaatcaatt
      841 aacttttcat gataacaaag aaaccgcttt atagattctt tccatgctgg tgtttccatc
      901 gttctcagta ttttaattgt ttcttccgtc aatctgtagc gatagttcgg actgttggtt
      961 gcttttccat tatcctctat caaagcagct gtcctgaatc tgtgcagagc ctgctttcta
     1021 aatgtttctc tgctgttttc tgcatagctc ataccgtaaa atgtgttaac aaactgaata
     1081 atatcatgaa tacgaatcca ctcatttgtt gcttcactcc atgacatatc cggctttatt
     1141 cctgccatag ccagtatcac atagcagcat atatctgcca gctgtgcttt aggcatacct
     1201 attgtttgca gaaattctct tgtttcctct attttcttat ccacagtatc cctccaatat
     1261 caaattgcaa tttgcctccg aataatcctt acttttaagc acttttctgc ccatttcctg
     1321 aatatcctct aactctggca caggcattgc atttatctct gttgaattaa cctgtgtaga
     1381 cccatttaaa atacgataat attcatcata aagtgtagaa ttaaataata catacaaccc
     1441 atacaccaga cattctgaca tttccgttaa tacaccctcc acaaaattaa ttttattctg
     1501 tgtacttatc ttttggtact gcggaaatct tttagctaaa tatactccac actgtaatct
     1561 tcgtggttct tcctttgctg taaatcgctt tacaaataga tagttcttgt tatcttgcat
     1621 taatcccttt tgttctgtca ccacatattc atgttctttc tggattggaa attctacctt
     1681 tccttgcttt atatgctgtg aataaaataa aggttttgcc ccttcttccg cttcatccct
     1741 caaaatatca cgattacgga aatctacggt caatcccgtt ttcattttta ctcctattgc
     1801 cggaagtgtc ttatcaaatt tatgcaattt ctttaatact tccacttcat tttcatctgt
     1861 cacaagataa acataataat ctgatcctgc tacaaccaaa tcatacggaa ctgttaatga
     1921 tgtcagctcc ccaaaatcac tatttgattt cgatgatgta atcgtaacgg tttctggctt
     1981 ttcacttgtc ttttttactt taataataat tgtttcctgc aacacacttt ctttgtcaaa
     2041 aaccttattt ctgctgacaa aaagatgaat atgtttcaat ttcccttctg tcagaaaata
     2101 ttcccggaaa cgtttaaaat acgctcctga tgtccatgat ctcggaatga tgtaaaccat
     2161 ctctccattt tcatataaat taaataatcc catagacgca aaaatgaaat ataaattcgg
     2221 tgcaccataa catacctctg gcatcgctgt tgcttctggt gcatctttcg gaattttcat
     2281 atacggcgga tttccaatta caaaatcata tttcttaggc tccgcatttc ctcccaacat
     2341 atgattaaaa tccaaatact gactcagaat atagttatct tcaattatat tgaccgtgat
     2401 ttttttcttt gtctcttctt tgcaatattc cagattacgt ttcaacaatg gcaatacatt
     2461 ttcatcgttc tcataacagg taagttcaat ctcctgaata gaatctattg tttccactcg
     2521 ctcaataaaa gcacatgata atatgcctga accagcccct gcatcaagca cacttacttt
     2581 tcctgttttc tcatcgaagt tatataatcc agacataaaa cgggcagttt ccatgctggt
     2641 aaaaaactgt ccgtattttt tacgttcttt tttcggcata ctctctatat attcgtttgt
     2701 aagttctata attttttcta gcatattcca cctacctaca agccttctat ttcttgctat
     2761 tatactatag cacagaccct ttttcaacgt ctcccaaaca tatgttctat tcttttattt
     2821 ccgttgtatt ataatcatta tcatctatat ctataacatc gtcgttactc tcaccttgta
     2881 cagaaaaatc tcctgtcata aaccatttgt atgcacgatc tgcgataacc ttcgtttcca
     2941 ctaaatccag tccgccacct acaatagcac ctactcctgg taacatcttt ccaagattta
     3001 caattccttt tgaaccaaat ttcgtaataa atctgaaacc aaccttctga ttaatcttcg
     3061 tcaagacctt gcccggtatc ttttttataa caccatttgc aaattttacg ccaaatttga
     3121 ttcctgcctg ttttaacaaa ctattcactg caactcctgc aagacaagca tatacaaagg
     3181 tttgtgtctg atcactattc aaatcataat cagccatata tgctgtacaa gctatcatcc
     3241 gcatttgtac ataaattaca cttccaatat tcgctggtat cgcaactggc attgttataa
     3301 agccaccaag tcctgttaca actcctgatg ttgtacattt ggcaatttga tttttcagca
     3361 tatccctaca agctttttcc tttgtctcgt gctttgacaa ataatcattt gccatatctt
     3421 caacaccggg actgaccatc ggtattccat ttaaacattt ttcatagcaa gaatctaata
     3481 atttcataat gtcttcttgc gtcatttttt tcttttccat atttcttata attccaatct
     3541 tcacattctt tataagctca tgaattatat ccaacgcaga catagttcta ttcaaatttt
     3601 atcataattg tgagaattgc tccatcctat tttcacaaac atgctccacc tacagtaaaa
     3661 aaaggtggca actataacca ccttttccat ttatctatat acgattttct tcaatacaat
     3721 ctcctccgca caagcccgga tattattcat tctcccaacc cattccatct gatcctgcat
     3781 cttcagttct tccgtcactc cctgcttttc tttcatctgc tccatcaatt tgtccatcat
     3841 ctgattacat tcctcgtcaa tctgatgcag atgctcattt aactttcctg caagcaccaa
     3901 ctccagatat aatcctttcc gatgctcttt caagtaagct tttctcagca ttccatactt
     3961 tccaatcgaa tactctgtct cttccgggag gtacagatct ggatagtaca gaccgtcctc
     4021 tcctaaagtg taactaattc cgttttctcc cataatgtgt ttctccatga ttgtcctcct
     4081 atcttactcc ccgatctttg ttctgactac gggtatgctg tttatttttc tgttcattct
     4141 gtatttcttg tttcttttct gccagctttg ctctaatacc atgtgctact ggctctttgc
     4201 ccgtttcctt cttccatttt tctattgcag tccgatactg gctatactca gtttgagaat
     4261 ccttaaaatc ttttaaaaat tcctgtacac ttctataacc gatcttctgc acaagccttg
     4321 gaaggtaatc tttcatatct ctgatctggc ttttcagttc aacaatttct ttctgcaatt
     4381 cttttttctt tcttccttta aaccatccct taacctctga cagttctttc ttctttgcag
     4441 aaagctgttt ttgtttctgt tgaattgcac cgttttgatc ttccagctct ttattcactt
     4501 taaatagttt cggatacttt gatgcaagcc tcgtcatttt aggttgttcc ggttctgcct
     4561 tttcttcttt tgttgtcttg actgttgccg tgctattttc agcaacatgc tttgggtgtt
     4621 ctgtttccgt ttgctctatc tgttttggca cttccctggg tttcttaaac agcacacttg
     4681 ctattaaaaa ctccaatgcc tcaacagcca ctgttacaac tctggaaaat aatcgtggtc
     4741 tgtttccacg ccgggcaatt gattccctga ttggctccgt gatttctttc cgcttcactt
     4801 ccattatctt cggttccggc actccactaa tcaacgccat atcaacagct ctgttccatt
     4861 cccggcgttt ttggttatct gcttctattt cctgtgcctt cggattattt tttccgattt
     4921 tctttgttgc aagataaaca ctgccacgct caaatacctg tagtttctgt ttatcgtcct
     4981 gcacataaat attgattaaa tctgtataag aatgctttac ctcatccaga aatgaattgc
     5041 acttgaagcg ttcatctttc gcagtaaaca gcttcttctc atggacttct cctttcttca
     5101 caatactgca gccttctcgg atctcgccat cttctcctaa tatctctttc ttggttcgga
     5161 catgttttcc attttcatca taaaacatat ttctgctggc aatcttaatg attggttcat
     5221 ccagtagttt ccgttctgca aatatcaaat gaatgtgata attggtcttt cttttattgt
     5281 ggtgcaaagc agcaatacac tctgttccat aattctgttt aaaatgattt gtaaaaagct
     5341 gcagcaatct atccggctga tattctgtaa aactctctgg caaagcaatg atcagttctc
     5401 ttgcctcaat acattttcct tctgttccac tttttgcaaa ttcttcctga ttacattttg
     5461 caagttcccg ccagaatttc cgttctgtgg tttcataaac tgcatagagg ttttcctgtt
     5521 ttgcatgact ggatatataa gtgatcctgc cttttacatt gcttagtttt gtcatttgaa
     5581 taaatgaatt tcttccgata ggcattccct ccttgtcttc ggcaaagtaa cttctgccat
     5641 attatttgtg agtggatgca ttggcatccg tttacgaact aactgccgtt tccggcacaa
     5701 atagctcccg gcagggcgaa atatcctgag tacaaatctt tgatttgtgc gatgggtgta
     5761 tttcgctctc aaacgccgag ggctttgtga gatactactt gcttatgcca taacttgtct
     5821 ttagtttttt attttgggcg tatccgccct ctttttctgc ccgtatccgg gcttattttt
     5881 tcactaacaa caaaccgact attactcttc ctgtctgctt tattccggta tccttgcccg
     5941 tacatactcc agatctccgc aatacggtgt tccaaccaaa taccattctc tcgccagatt
     6001 catttccatt cgtgtcttta cccactgatc atcaactaga acttccagac attctccaca
     6061 gtgaaatccc gtatcaatcc ataagtctga tgacaataat ccatatcttt tgttataact
     6121 gttgtaacct aatcttcctt ctctcatggt gtcattctcc cttcatttat catttccgca
     6181 gagacgccct gaatcagggc ccccttttga aaagtttttt cttcgccaat caactaccgg
     6241 ctacacctta cagaccttct ctttgtctgc tatctacagg tgcgtggcac gctcctgtca
     6301 taaggtaatc aagcagaatc tttaccaacg aaagttctca tgcattccac acttatccaa
     6361 atactcttgt ctggcctttt ctcgttcttc ctcggttggt gctgttttga atgttgtata
     6421 taactgacgc tttaccatac tgtctaattt ttccatcaat gctttttcta tctccggcaa
     6481 atattcctcg ttcctggcaa gatgatactg gaacaatgcc agtaataaat cttttggtat
     6541 ctgtacctgt aattttcttt ccatgtttct cctttccgtg acggtcgtga cgatagtgac
     6601 gatattttcg ctaccgtcac gctatataaa ataccgtcac aaccgtcaca tatcgtcacg
     6661 cttgttttcc aacaaaggtc aatctgattt ttcttccatc gtgtcctcgt tctgttttat
     6721 actggattcc atattccaga atcaatcgtt ccaggctgac atttaatttt cgtgataatg
     6781 cgtttggctg catttccgcc tgcaggattt gtaccaactc tgttgctgta cctgtccact
     6841 ctggctgatc tattgtgaca agttctttga ttttctctaa cagaggatct tctggttctt
     6901 tccaaagttc tgtctctgtc ttggtaagtt cccagacaca tctctcccgg ttaaagttca
     6961 accatagttt ctgatcctgc tggtcacgcc ctgccacttc cagaactgct ttattatctg
     7021 tacgcttttc tttctgcata acaaacgctc catccgcagc tccaagcaat ccattcgtac
     7081 ctgaaatcat atcgaaacta tctgaagatt ccattttcct tgtatgatgc acaaccagaa
     7141 aacagatgcc atatttatca ctgaatgctt tcagctttgt tacaatctca taatcgctgg
     7201 cataactgaa tttatctcca ccgacctctc gaaccttctg aagagtatca atgataatca
     7261 gtcttgcatc tttatgctct gtcacaaacg ttacaagctg cttttccagt ccgtcattga
     7321 gtgatttgga ttttgtagca aaataaaaat tctcactgcc ttccactcca aacatctgtg
     7381 ataaccgttt ttgcaatctt gcataatcat cttccaatgc caggtacaga accgttccct
     7441 gtctgactga aaagccccac aatggaattc ctttactgat gtgatacccg atctgtgcca
     7501 taaagaatga ttttccaatt ttcggtgaac caacaaacag gtacgttccg ttatacagaa
     7561 gtccatctat aatcggcagt tttggtggat atgcggtgtc gtataattcc atcatagaaa
     7621 ctgtttccag atcatctgga ttctttttat ttgctgattt tgctgtattt tccacattat
     7681 ttgtggcttg cagattgccc ttaccgggca aatctgctat actttggttg gttaatttat
     7741 tacttgatga ctgttccaca tctgcgccaa cagatgcaat cggagcagtc attttttctt
     7801 tttctgtcat tcattttccc ctttcaatcc atacaatgtc tctgcaacca atctcagggt
     7861 actcagcaac tcttcgtttt cctcactgca tttttccaga cgtttcagtt cttcataaat
     7921 ctctgccatc tgatttcgca atgctttata gactcttggg ttcccctgta ccacaatttc
     7981 ccgatcagtt aatcgttttg tgatatagtc ctgcttgcta agtcctgaca atcggacaca
     8041 tgcatcaatc tgctctgctt cttccggtga tacccggaat gctacgattt tgtttctcca
     8101 tcttccttta ctgtccagat tcttctccat ttgatttttc catcctttca atatccaact
     8161 gttctgccat ctgtgtctgc tcacttggaa acaaatgtgc atatcggtaa gtgatatcaa
     8221 tgctctcatg tcccattcga tctgcaattg ctaataccgt aaatcccata ttgatcaata
     8281 acgatacatg ggaatgtctc aaatcatgca cacggattcg cttcacccca gacacttttg
     8341 atcctttgtt catttcatga tgcagataag atttggaaat cgtgaaaatc cgttcgtcct
     8401 ctttcggctc atacagcatt ttcagataat cctgcatctc atcacatata aattgtggca
     8461 tcttgataaa tctgttactt ttaaatgtct ttggtggggt aatcacatct tcccggttta
     8521 atctttgata tgatttatta attcgcagtg tatgcgttgt aaagtcaaaa tccgctggtg
     8581 tcagggcaag caactcgcct tcacggatac cacaccagta aagcatttca aaagcgtagt
     8641 aagaaactgg tttatccatc atcacatctg caaatttcag atattcttct tttgtccaga
     8701 acagcatttc cttatattcc tctattgcta tatttccagc tttctgtgcc ggattagaag
     8761 gcaaatgata aaaatcaact gcatgattga aaatggcact taactggctg tggattttcc
     8821 gcagatatga agcagaatac ccttttccat ttgctctctt aaatgcaagc aaatgattct
     8881 gccatgcaat cacatctttt ggttcaattt ctgcaatctt tctctcccca aagtatggaa
     8941 gaatttttgt tctggcaata ttattcttag ttccccatgt gttatcccgg attcgttcct
     9001 ttttatcttc tacatagatt tcataaaaac ttgcgaaagt catatccagt tttgcatcat
     9061 gctttaacat ttgttcgtgt tcccacatct gcgcttctcg cttcgtagga aatcctcgtt
     9121 ttgaagtctg tttcgttttc cctgtccagt cggtataccg ataaaccact cgccacttcc
     9181 cagtaacatt atctttgtaa atactcatac aaattctcct ctctcttaca cttcgctgta
     9241 acatactttt ttgcggaaat aattggtgtt tactctgcca gctactgtaa gataacccaa
     9301 cttctgtaat tcttcattta gctgtttgac aatcttataa gcataagact tggaaacccc
     9361 cagttccgct gcaacttctt ctactgtcat aaaagttctg ttcgtcacat cgcatccctc
     9421 ctttcttctt aacgcatttt gtttaggttg ttgcatttgc atgatactaa acttttttat
     9481 ttaagtcaag taatttaacg agaatattaa atttttttgt ttaagttctt cttgctatcc
     9541 gaaattgctc atgctatact gaacctaaac agaattgttt tattttctat cacaattaat
     9601 tatggcaaca cgaatgacca ctataaggag gcaaacaaaa atggcaatcg gacaacgtat
     9661 caaatatttt cgcaaccgca aaggcatgac acaaaagcaa ctcggagaac aactgggatt
     9721 catgggaaaa acctcagatg tccgcatggc tcagtatgaa tctgaagcca gagttcctaa
     9781 gattgatctt gtaaaacaaa tgtctcagat ctttggtgtc aacacacatg ctttaactgt
     9841 gcccgatatt gatactcaca taggtctgat gcatacacta tttgcactgg aagatatgta
     9901 tggtctgaag gtaaaaaatg tagatggaca acctcatctc tgccttgact cttccatatc
     9961 tgctcccggc tcatctgttg atgaaatgct tcgtgcatgg atggaccaga ctgacaaatt
    10021 ggaaaacggt gaaatcagca aggaagaata tgataaatgg agatataagt acccggaact
    10081 ggatacttac cagaaacgtg ccaaggtacc atctcaggaa ctgagtgatt acctcgtaaa
    10141 agaattgact aaaaaagaaa agtaaaagaa gaaaatagaa aaaccttacc gatattcagt
    10201 tttttacttc tgaaaatcag taaggtttct ttatatacgt tatgaaataa aatttttatc
    10261 ttttgaataa tcacaaaatg ttgccatttt gttgccactc actaagcaaa tttgcttgaa
    10321 acccgcataa gtactggtat cttatgattg tgtggattat tcaaactcga ttgttccagg
    10381 aggcttacta gtaaggtcat acattacacg gttaacccca cgaacctcat tgataatacg
    10441 agacataact ttatttaata cttcatatgg aatctgagca gattcagcag tcataaagtc
    10501 aattgtatta acggcacgta aagcgatggc ataatcatat gttctttcat cacccataac
    10561 acctactgaa cgcatatttg tcaatgctgc aaagtattga ccgattgaac aatccaatcc
    10621 agcatttgca atttcttcac gataaatggc atctgcatct tgtacgattt ttactttttc
    10681 ttcagttact tcaccaatga tacgaattcc taaaccaggt cctgggaatg gttgtctgaa
    10741 taccaagtat tcaggaatac ctaattctaa accagcttta cgaacttcat ccttgaacaa
    10801 gtcacgcaat ggttcaataa tttctttgaa atctacgtga tcaggaagtc caccaacgtt
    10861 gtggtgagat ttaattacag cagattctcc acctaaacca ctctcaacaa catctggata
    10921 aattgttccc tgaactaaga aatcaacttt tccaattttc ttagcttctt cttcaaatac
    10981 acgaatgaat tcttcaccga tgatcttacg ctttgcttct ggttcttcaa caccctttag
    11041 cttttcataa tatctttgtt gcgcatttac acgaatgaag tttaattcat atggaccttc
    11101 aggaccaaat acagcctcta cttcatcccc ttcgttttta cgaagtaaac catgatcaac
    11161 aaatacacat gtcaattggt taccaattgc ttttgataat aatacagctg caacagaaga
    11221 gtctactcca ccactaaggg cacataaaac ttttccatct ccaacctttt cacgaatagc
    11281 cttaatttgt tcttcaacaa agttatccat tctccaatca ccacagcatc cacaaacacc
    11341 acgaacaaag ttatataaca tttttgaacc ttcttgtgta tgtaatactt ctggatggaa
    11401 ctgaattgca tataatcctt tttctgcatt ttcacttgat gcgcaaggac agttatctgt
    11461 atgagacgta atttcaaatc caggtgctac ctgtgaaata tagtcaaagt gtgacatcca
    11521 acaaattgtt ttttcagata caccctcaaa gattttagaa gaagtcttat ccacaagaac
    11581 ctctgtctta ccatattcac ggtgatccgc tttttctact tttccaccca atacatgcat
    11641 catcaattgt gcgccatagc aaagaccaag aactgggata cccaattcaa ataattcctt
    11701 tgaatatgta ggtgaatctg cctcataaca actatttggc cctccagtta agataattcc
    11761 ttttggattc atagctttga ttttttctaa atctgtttta tatgaataaa tttcacaata
    11821 tacattgcac tcacgaacac gacgtgcaac caactgatta tattgtcctc caaaatcaag
    11881 aacaatgact aattcttttt tcattacgtc ctcctattaa tttctcaaca atattataaa
    11941 gtagcaacca attatttaca acgctttaaa taatcaatta cttcatttaa atccgtacaa
    12001 gattcttgga tatactcctt catttcgtca tctggcgcaa tctgatcttg aataaaaatg
    12061 gaatgcatct tcgcattggc tgctgccata attccatgct tactatcctc taatactaaa
    12121 cagttttctg gttccattcc taatcttttt gctgcagcca agaaaacatc tggagccggc
    12181 tttgatttat atccctcttt agaagataca atcacatttg gacttaacac aaaacctgca
    12241 tgcgacaaga actttttaat atcctgcggg tgcgaacttg aagccaccgc ataaggaatc
    12301 ttattctctt caatatactc aattagattc tgtaaaccaa ccatattggc actaccaggc
    12361 tctttaaaat atgtaaagaa ataatccgca cgctctttct gaatttcttc cataactctt
    12421 gtcacacctg gatacatcat ttcaaactct gcaacctttc ttgaatcaca accaattaaa
    12481 ggttctggaa aatagtctgg acaagaaacg cctaattctt tagccttctc tttaaactca
    12541 tccttaaaca tcgtctccgt attaaacatc aaaccatcca tatcaaagat aactgcttta
    12601 atttgtggct ttctaaaata ttcaatcaaa cactgcgtgc ctaaatcact cataccacga
    12661 tccattagat ccacatattc tttgatgacg cgatttacac catacaaagg aaaatttgcc
    12721 tcttgtactg caatttttaa atccttcaca aaatgcttca cataaaaacc aggcgcataa
    12781 tcattctcta acatcttctt accttggaat tccaattgtt tagatccagc agcaccatta
    12841 cgtaagaagc gatacacgaa agattcatcc acatctttac atttcaaata cgacatggcc
    12901 tcacaaatac cttgtaacgt attcgcaatc atgatttggt tagccatctt gacatgttgt
    12961 ccacaactcg ccttgccaca atactcaatt tgtgttccaa atgcttcaaa gatttcttta
    13021 tttgtttcaa aatcctcttt gtctgcacca accaaaacag ttaaagtacc atttttagct
    13081 cccgtatctc caccagtcac tggtgcatcc aatacatgaa cgcccaattc ttttcctttt
    13141 gtatacaatt ctttagctaa ctttggactt gaagtcgtaa aatcaataca agttttaccc
    13201 gtttcaatac aactaaaaat cacatcatat acttctttaa catcactagg aaaaccgacc
    13261 attgtacaaa tcacatctac attggatgcc aaagacacaa tcgattcttc taaaatggca
    13321 cctgcatttt tagcttgttc taccctttct ggatgtcttg cgtacacatg cacttcataa
    13381 cccttattca ataaatgaat aaccattggc tggcccataa tgccagcacc aatccaacct
    13441 atttttctca ttttactttc ttcctttcac taatattgga gcggattgca ccgcaataaa
    13501 tttcacaacc tcaaacataa acttaatttc ttctgcattc aattgacgaa tctccttaat
    13561 tccagacaat gcctgtttaa atgaaaattc agttaaatcc gcaatcgtat aaatttccat
    13621 acgatcaaat aattctccaa tcaaatgaca ataacgctct ttttcttcca gtggacgatt
    13681 cattaataac ttatttacat attccaataa atcatcactc tcttttgtga gtgcatcaca
    13741 ataattaaag tcttttacat ccacactcca cataagcgta tcatgctgaa ctagaccaga
    13801 accttcggca tccatgactt tcgtttgttc tctatgatct aaaacacgac ccacaatgct
    13861 atccttagga atatagtttg tgatcttaga tacatcctca tcaccttgcc aaaaaccatc
    13921 cgcaaagcca ggcccatcaa aacttttcac accttcaata tatccctgta gttctggatt
    13981 ttttaaatag gcatacatcg ccagatttcc accctttgaa tgccccgtca aatacatctt
    14041 tggatagact tttttctttt ttaatccaaa caaataactc gtttcaggaa tatccgcaat
    14101 agtttctttg acataatcct tcgccaggat ttgacaagga agatccttgc tataggtcat
    14161 cattaaattt tctttccaac ccgtaatact accatctgta ccactaaaag aaataacgaa
    14221 agatccatct ggcaacacaa gtgttaccac actaaattga atcacatctt catcgctata
    14281 gactttttta tagttcttta actttatatt tccataacga atcgtatctt gtaatgcatc
    14341 aattaaatgt ggaagattca ttgaaaatgt atacttactt tttaattcct cttcactata
    14401 taaagaaaga tatttacgac atgcttcctc taaagaaacc tcatcctttt ccacaatgcc
    14461 atcccaatcc acataagata aatcggcaca tactaacata tccacctcat taaagggacg
    14521 aacagaaaag tcgatatctc ttcttaaatg tacataattc aataagtttt ccataatcaa
    14581 cctcactaca tcgattttac aatatacaaa aggattagcc aaatgactaa cccaattttt
    14641 tcgataattt tatataatga tctgcatttg aaagaatgtc agaaacctga gattctgaaa
    14701 tctttttaat gatcttagcc ggtactccaa ccacaacact cttttgtgga atcaccatat
    14761 tttcaggtac aacacaacca gcaccaatta tactatggct tcctatacaa gctccattta
    14821 agataatagc gcccatacca ataagcacct catcctcaat cgtacaacca tgcacaatgg
    14881 ctccatgtcc aatcgtaaca cgattaccaa tttttaatgg ataaccacaa tccgtatgca
    14941 aaacacactg atcttgaata ttgctttctt gcccaatttc aatcgtttct tcttcactac
    15001 gaatcacggc gttataccaa acacttgaac ctttgccaat tgaaacatta cctaagatgg
    15061 ttgcattact tgcacaagaa aaatcctttt taacctcatc cagtctctta caaagccaaa
    15121 ctacaccatg ccacacatca aatttcatac cacaagatgg caatgcacca tacatagtaa
    15181 agccatgttt tttatgaaaa gctatacttt cttcattcga atctgtaatg catgaaatga
    15241 tccaacgcat gtttaatttt ctgcaatccg cttcaagctg atctaacatt tttgaaggca
    15301 aaccataatg ttttccttct ttaaaataaa tggtaagttc tacacaatac ttatacgcct
    15361 ctttagaaaa agccgggtgc acatagccat agccaattag aacatcatca ttaagtgcga
    15421 cataaaatgg atttgtcttt gaaacttcca tcatatgttc tttaaataca tcaaaaggat
    15481 ctggcacaac atcaaaaatc gcaacgcttg tctttacata atgtacatag attggatata
    15541 tactttctaa atctttttct tgaagtaaac gtagctcttg cataaaacaa cctattggat
    15601 ttctagactt gaatcggcag ttaaacttgc atctgctctt cttccagaaa gataggcgat
    15661 ggttcctgca acaccgatca tacttgcatt gtctgtacaa caatacattg gaggcacggt
    15721 aaactcaact tctggatatt catttcttaa ttcttctact ttttcacgaa gtcttgagtt
    15781 ggcacttact ccaccaccta caacgaaatg gcgaatatct ttatgatcat ctaatacggc
    15841 tcttactcga gagaaaattt catccaatgc acattcttgg aatgaacaag ctaaatctgc
    15901 catatcaaat gtcttgcctt gtctttccat acgtttcgta aattgtagaa cactactctt
    15961 caatccagaa aagctaaaat cataacgacc ctgtgttttc ggttttgcca gttcatatac
    16021 tggctttcct tcttttgcca atttatcaat tttaggacca ccaggatatc caagacccaa
    16081 tacacgagcc accttatcaa aggcttctcc aatcgcatca tcctgtgttt ctcctaagat
    16141 ttcaaaactt gtttcatcct tcatataaac aagttctgta tttccaccag aaacaacaag
    16201 agccaataca ggatatttca tatccaccac taattcattg gcataaatat gccctgccaa
    16261 atgatgcact ggaactaatg gtttatggta ggcaaaagcc aaagtcttgg cagcttgcac
    16321 accaacgtgt aaacatccaa ttaaaccagg accttgcgta acggcaaccg catccacatc
    16381 ttccatagtg atattggcat ccttcaatgc tttttcaata caatacgaaa tattttctac
    16441 atgaatacga ctcgcaactt cgggtacgac cccaccaaat tctgtatgca catcaatttg
    16501 gcttgcgact acacttgata atacttcctt tttatctttg acaaccgcaa cggctgtttc
    16561 atcacaacta ctttcaattc cgataataat catataatcc tcctacaatg ccaatgtcat
    16621 cacaatggca tcttctccat ctggataata acttttagat acattgactt gaataaagcc
    16681 cattttttga tacaaattca aggctgcctc gttactagca cgaacctcta atgtcatata
    16741 ttcacaagcc acttctcgag ctcgatcaaa taaatgcatc atcaattctt taccatatcc
    16801 tctttttctt aagtcaggat cgactccaat acgagcaagc tgcgccattt caaaggtttc
    16861 ccataaaaag gcgtatccaa caatgatgcc ttcatctagt aagatccaac catgagaaaa
    16921 aggattctca ttcaattcat ataaacaatc tttttctagc caaggctgtt taaaacatgc
    16981 ttgttcaagc tctaaaacac gaactagatc cgtgtctttc atttctttaa aaatcatgct
    17041 ttataggcat cgctttcttt taaatattga ggaactaagg catgtacatt ttcaattgta
    17101 cgacttggta cctccataaa attcttcaag aaatcacaac ttgcagcttc ttgattcact
    17161 aaatatccat ctccatataa agttccagga tgttcgttta agaaatcttc caattgatcg
    17221 acttctaaaa tttgtgtttt tccaaccaca acaccctttt ctaagtgtgc tacatacgct
    17281 cttttactac gcgcatccaa aataacattg gcactttcat ccatgcccgc aaacaactgc
    17341 attgtactaa tacactttaa tgttttatgc atttgtgtac aaaatacttt cgcaattgtc
    17401 atcgcaatac gaatacctgt ataagatcca ggaccatctg taatgatcac ttcatctacg
    17461 tctttatagt ctaaacctgt cttttcaaac aattgattta attccacaaa aatcgtttca
    17521 ctttgtttct tgaatgcttc aatagataca ccatcaatca atgttccatc tttatataaa
    17581 cctaacacta atgctttata agcactatcc atacataaac taatcatgat atgcctccac
    17641 aatactcttt gatacaggac ccgtatattc taatgtcact gtacgatttt catcgatacc
    17701 ttcttcaaaa gaaattttga tatgatcttg tggcaattga tcttcaataa attcagacca
    17761 ttctactaca gtaattcctt catcaaaaca atcttcaaaa cctaagtcct gacattttcc
    17821 ttctaaacga tacgcatcaa tatgatggaa agggactcca ccttcttgtt tataatcctt
    17881 taagattgta aaagttggtg aattgatcac attctttaca cccaaagctt tcccgaagct
    17941 ttttgtccaa gttgttttac ctgcacccaa atctccatct aatgtaatga ctaattgttt
    18001 atttctacaa agatctgcca ttttagtggc aaattcctgt gtttcttgta aactatgaat
    18061 ttctattttc ataataacct ctttctactg aaatacacta ttccatctta tatctttttt
    18121 caactatttt ttcttcatca tacgatttct atgcaccatg gccttgattt gtttaatctg
    18181 cgcaagacga ttacgcacct tcacttcaga tccctgatca cttggtacat aatattcttt
    18241 accaactaga gcatctggaa gacattgcat atccgtcatg tgatattcat aatcatgtga
    18301 atactcatat cctttactgt agcctaaatc tttcattaac tttgtcggtg cattacgaat
    18361 gtgtaaaggc actggctgat ctaatgtttt tagtgcatca cttttcgcat tattataagc
    18421 cacttctaaa gcgtttgatt ttggagctaa agccaaatat acagctgcat gcgttaaatg
    18481 tacattgcat tctggaacac ctaaatagtg acatgcctga taggctgcca cacagatttc
    18541 taaggctcta gaatctgcca taccaatatc ttcactagcc atacgaacga atcttcttgc
    18601 gatatataaa gggtcttctc cagcctctaa catacgtgct aaccaatata cagcagcctg
    18661 tacatcggag tttcgtacac ttttatgtag agccgaaata atattataat gttcctcccc
    18721 ttttttatca tacaataacg accttttttg aaggcactgc ttcaaaatat cctgtgaaac
    18781 atgaattcca tccaattcac ttggactatt atatacaacc atctctaatg tatttaaaca
    18841 aaagcgggca tccccacctg aaaatgcggc aatcgcttct atatcttcat ccgtaatatg
    18901 tatttttaaa ttgcctaatc cttttgagct ggaaatagca cgaataatca aatcttttaa
    18961 gtcatccgta tttagactgt ttaacacaaa cactctacac ctagataaaa gggcagaatt
    19021 aatctcaaaa ctaggatttt ctgttgtagc accaattaga atgatacttc ccttttccac
    19081 atagggtaaa aatgcatctt gttgcgcctt attaaaacga tggatttcat ctacgaatag
    19141 tattgttttt tgtcccaagg ccctacacgt atcggcctgt ttcataactt ccttgatttc
    19201 tttaatgcca ctagtaacag ctgagaaatc cacaaaataa ctctttgtct gatgggcaat
    19261 aatacgtgcc aatgtcgttt ttcctactcc tggtggaccc cagaaaatca tcgatgtcac
    19321 ttgatcatgt tcaatcattt gccacaaaag atttcccttg gcaacaagct gtttttgtcc
    19381 tacatattca tccaatgttt ccggacgcat acgatcggct aaaggccttg tatcttcttg
    19441 tatcgaaaat agagaatctt gttccatatg tttcctccgt atgtacttta ttatacacca
    19501 cacaaaagaa aaagtgtttg accttaatca aacaccatct tctttttctc aattcgtctt
    19561 aaaaataccc atcccacaac acaagataaa acttccgtaa tcacaaatgc ataccaaatc
    19621 aaagatacat ccctatcata ttgaatgact aattttgaaa atccccaagc caaaggtaaa
    19681 atcaaaatgg cttgtctaag caaggatacg atcaaggatt ccatacctcc atttaaagct
    19741 tgatacacac cttgaaatgc gatatttaca cccgcaaaca caaagctaac ggatatgatt
    19801 cgaacagcac tgatgaaata tattctggat tgtcctgcat taaatagtgt cgcaaaaaag
    19861 tttgggaaca gttctgtaat tagagtaccc aagaccatta atactatagt atagattaag
    19921 ccatacttga ttccatcctt aacacgctgc ttattttgca taccatacga aaaggcaatg
    19981 ataggtgtaa tcgcatctct taaaccgaat gccataaata atacaaattg ttgtacttta
    20041 tagaaaagtc cataggctgt ttgtgcagat gcatcaaatt ttagaattaa gttcatcgca
    20101 tacaccataa atgacatcaa tgcctgagca ataatggctg gaagtccaat cgaatagatt
    20161 tcattgatga tacgcttatt gggttttgta taagtgaggc catgatcaaa ttctttattt
    20221 aaacgcatat gaaacacaac taataataga gctgataaaa tttgtccaat gactgtcgca
    20281 taagccgctc cttgtacacc taaattgaaa ccatagatta atacgggatc tagaatcata
    20341 ttgattacgg ctcctgtaac ttgtccaatt gtagaatata aggagcgtcc tgtagcttgg
    20401 agcagttttt caaatataga aaagaacaca acgccaaagg ataataggca acaaatcctt
    20461 agatagtcta cactcatcga taatacaagc gcattccttg tttgagagga tatataggct
    20521 ttaatgccaa acaatccaaa caacacgcaa ataatataaa taactgtagc tagaaacaaa
    20581 ccatttccgg ctactttaga agctttttca cgatttctct gccccaaagt tcttgcgagt
    20641 aaggcattta ctccaacacc tgtaccaatg gctaaggcca ccatcaacat ctggatagga
    20701 aatactaatg taagtgcatt taaagcggct tcacttcctt ctttcatatt tcctacaaag
    20761 gcactgtcca caatattata gacagcttgc aaagccatag ataaaatcat gggcacaccc
    20821 atttgaagca ttaacttatt gacgggctca tttttcattt tgttgttttc ttccattctt
    20881 ttcctcctta aaacacaaaa aagtgcattg catacataag ttggacttac gcttttaagc
    20941 aacacacttt acaaaaatat aaaaaagtag cagaaaactg gatttacgct gtttccgcta
    21001 ctcgttacga agtgattata tttttatttt tttaatttgt caaagtaaaa ttacatggct
    21061 ttttcaatta attctacaca tttatcaatg cccaaaacac cactgtctaa acacaattga
    21121 tagtttttgg catcggccca tttacgatct gtgtaatatt tatagtaagc aatacgacgt
    21181 ttatctttgt ccttgattcg tttgtctggg gcaaattcat tttgtccata aaccgaaaca
    21241 acacgattct tgcgatattc aaagtccgca tgaataaata catttaatac gtctttacga
    21301 tcacgaagaa tatagtctga acatcttcct acaataacac agttgccttt atcggctaac
    21361 tcacaaatta catttctttg cgcaacatag agttcatccg ataagttata tccataatta
    21421 ttccaaataa aaccaaatgt acttgtgctt gatgcatctt ccccattttc ttcaatatac
    21481 tctttcgcaa aaccactttc ttgagcaatt tgttgcacaa tttcataatc ataataagca
    21541 tagcctaatt tttcagctaa tttcttggca atggttcttc cgccacttcc aaattcacgt
    21601 gtaattgtaa ttatcataag atacctccta atatacaaaa atcgagcctt ctacaactgg
    21661 atttatatcc cgcaagaaga ctcgttgttg tagatatttt accaaaatta ttaagttttt
    21721 gtaagtaatg gtacagcata ttatatacac catcacctcg cttttatata ctgcactatc
    21781 tacttatatt ttaattcaca atagatcaaa agtaacgtaa aatgttgcat tttaacgcaa
    21841 gaaaagcata acatttaaat ttactttata taaagcactt atagatatac ataccgcagt
    21901 gcgcaaagta atttagtgca aaatataccg caatgcgcaa agtatataaa aagaatagaa
    21961 tctttcgacc ctattcctta gctccatatt cttcataata tgcatcaatt cttgatttta
    22021 attcaggatc taatttatca cctaaatatt caattacttc tggcattgac acgattgcag
    22081 ctgttgggaa accatataat tcactcactt ctgttagtgc cgttttttca ccttttccct
    22141 tttcgttacg atttaaagaa accatcaatc caacgatttc aacatttgct gcgccacgaa
    22201 cctttggtac tgtttcttcc attgattttc cagatgtcgt aacatcttca atcataacga
    22261 cacgatcacc atcttgtagt tttgttccta agaaacttcc cttatcagct ccatgatctt
    22321 tttcttcttt acgatctgaa cagtaacgaa cttccattcc atataaatta tctaatgcga
    22381 ttgtcgtcgc aacagccaat ggaattccct tatatgcagg tccgaataat acatcaaagt
    22441 ctttaccata ttgattgacg atcgcctttg cgtaatattc acctaatctt tttaactggt
    22501 tacctgtaac atatccacca gcattcataa agaacgggga tttacgtcct gattttagtg
    22561 taaaatcacc aaattttaac actttagatt ccaacatgaa atcaataaac tcttttttat
    22621 attgttccat tttcttgcct ccacttttac agaaattcta tcatacttct gcacaatttc
    22681 ccataattgt atgattttta aatgataaat attatggtag tatagagctg aggtgataga
    22741 caatgcagga aaatgtattc ttttttctga aggctaaaaa tagtgttaaa tatttatacg
    22801 ataatatcag tgtagaagat ggacttgccc tcatgcgcaa gcacaaatat acggcgatgc
    22861 ccattattaa taaggaagga gtttatgtag gaaccattac agagggggat tttctttggt
    22921 acttattaga tcatccttca attcataaag aagatctaga acaaatggag attcatgaat
    22981 taattcgtgg agactttacg ccaccggttc aaatcgatgt tccagtcgat caattgttcg
    23041 ctcaatcttt gaaacaaaac ttcgtacccg tagttgatga tcgtcaaatt tttattggta
    23101 ttgtgacacg acaatccatc attcaatatt taatgaataa cgcaagtaaa tatctataat
    23161 tttaaaacag ccagattttc tggttgtttt ctttatcaca ttgaaaaaag cctttaaatt
    23221 gtgtatgata atgggtatta atgaaggagt gatagtttca tgaatccgga tgattcagtc
    23281 ccgttacagt atagtttagg agggttaata tgaaccttcc catcttaatt ttatttacat
    23341 ttattctaaa catcttgttt tcaatgacaa gcagcatcgt ttctacaaat aaagaaatcg
    23401 aagatgaatc ttataaatat caagctacat taaaatggtt atctctatgc atggaagtat
    23461 ttggcacttt gatgagtgta atctatttac aatctttgat tcatggtcca ttactttatg
    23521 tagtcgtact tctattggtg tatgtatata ttttattgag tgatttatta ccaagaaaat
    23581 ttgcgaatgc tcatttagac aagtttgaaa aaacatttat gtccattgcg aaaggcattc
    23641 aatccttatt tacaccattt actttcttct tgcgttttga agtagaaaaa gaacaagaag
    23701 attattctga agaagatatt tatgaagtta tcaacggtgg tggagtagaa ccaagtcaaa
    23761 aagaatttat cgaaaactta tttgaatttg atgatacacc cgttgaagaa atttgtacgc
    23821 atcgaagtga agttgtttgt ctatatctaa atgatgataa agaaacatgg aaaaagacca
    23881 ttctagaaaa tcgtcacaca ttataccctg tatgtgatga ggataatgat gatgtcgtag
    23941 gtattctaga tacacgtgat tatttccgtt tagattcgat tgaacaagat aatgtcatca
    24001 ataaagcaat ggatcagcct ttctttattt cacaaaatat gaaggccgat gttttattga
    24061 aggaaatgaa aatcaaaaaa gtatactttg cagttttact ggatgaatac ggaggaatga
    24121 ccggtatcgt gactttacac gatattattg aaacccttct tggtgaaatc caagaagatg
    24181 atgatattga agagccagat ccaatccaac aaattgatgc cgatcaattt agaatctatg
    24241 gccaagctga cattgaagat gtcgaaaaag ctttaggcat ttcattagaa gatgaggatt
    24301 gtgacacatt tggtggatac atcctaaatc attatggaca aattcctgat gaaggttcac
    24361 attttaaagt cagtctagac gtaatggatg tatatgttaa ggaagtaaaa aatcatcgta
    24421 tcggacaaac tattgtacaa ataaaacgaa aagaggaagg aaaccaacat gaaagcactg
    24481 aaaaacgcaa tcgagactag aggacatttt atcaatgatg aagtattaaa ggtagatgct
    24541 tttttaaatc accaaattga ttgttcttta gtagatgaaa tgggaaatga attttatgaa
    24601 cactttaaag acaaaggcat cacaaaagta gtaaccattg aatctagtgg tattgcccca
    24661 gcctatgcga cagccatccg tttgaatgtg ccattgattt tcatcaagaa aacacaacca
    24721 agcacaatga ttgatcctgc ttttgcggaa gtcttctctt tcacaaagaa taagacatat
    24781 ccaatttgta ttgaaaaaga atacttaaaa aaaggcgata aagttttatt cattgatgat
    24841 ttcttggcaa atggtgaagc attcaaagga acagaaaact taattgcgca ggcacaagct
    24901 gaaattgttg gtgttggcat ggtgattgaa aaagctttcc aaaaagggca tgcatatatt
    24961 gtcgaaaagg gatatgacct tttggcgttg gcatctattg caggtgttaa gaataagaaa
    25021 attacatggg ctgatgaata atcagtccat ttttttaaaa attcggggga ttctaatgaa
    25081 aaatacattc aaaaaactat ttattagctt taccgtatta tcactcactg catgtagttc
    25141 aacgacaatg caagacaata cgtttgtagc taaagaaaac aaagacttca caaaatacac
    25201 aaaccaattg gtagttgaat atgccgaaaa cgactataca acgatgcacc aattcttcga
    25261 acatccagaa gactatggta ttgatattaa taatgtagat gtaagtcttg gtgaatttta
    25321 tacagacaat actgaagaaa tcaaagaaga tcaaaaagaa ttaaacaagt ttaatcgcaa
    25381 tactttagac aagacgcaac aaaccattta tgatgaatta aagtatgaga atgaattgag
    25441 cttaaaagct gaaaacaaga aatttcaata tctaaacaat tg
//
LOCUS       DBMDWL010000008        24707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_752_length_24707_cov_2.846379, whole genome shotgun sequence.
ACCESSION   DBMDWL010000008 DBMDWL010000000
VERSION     DBMDWL010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 24707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24707
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_752_length_24707_cov_2.846379"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="ACZPDN_01075"
     CDS             <1..898
                     /locus_tag="ACZPDN_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HIR1297251.1"
                     /translation="EELPLNFIDIILDTIHDTWAMLPLLYITYCILEVFERKNTQDDK
                     LFFGLQRLGPILGAIVGLIPQCGFSILAAMLFVQNNITLGTLVSVMIATSDEAIPILI
                     SNPKMYSSLLGILGLKLVIAILVGLFTDHVLFRKQKILRFEDMEEEDYDEDEYEEEEN
                     SGSSCPCCYPEYPLWISALLRSLKIYVFIFITSFVLTLLMECIGEKTLSMILLQDNLF
                     QPIIAALFGFIPNCAATVVLTQLYTAGELSFGSLLAGLVTNAGLGLIVMIRYEARKKD
                     ILKTISILFITGVIVGIGFMLL"
     gene            complement(919..1113)
                     /locus_tag="ACZPDN_01080"
     CDS             complement(919..1113)
                     /locus_tag="ACZPDN_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297252.1"
                     /translation="MSWLDKIAKKRLAELEEHPELNEQNYSQKLNSIIFYILLFAIAI
                     LILLFWYDIEHFEIWIFPQR"
     gene            complement(1126..1347)
                     /locus_tag="ACZPDN_01085"
     CDS             complement(1126..1347)
                     /locus_tag="ACZPDN_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297253.1"
                     /translation="MSWIERTTKKRIKELDERPELNSNDFEEQGNKMMKDIKFYVIVI
                     LLAALVLALWYQWNHPEIDIFPFHFPILV"
     gene            complement(1481..1567)
                     /locus_tag="ACZPDN_01090"
     tRNA            complement(1481..1567)
                     /locus_tag="ACZPDN_01090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1531..1533),aa:Leu,seq:taa)
     gene            complement(1575..1648)
                     /locus_tag="ACZPDN_01095"
     tRNA            complement(1575..1648)
                     /locus_tag="ACZPDN_01095"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1614..1616),aa:Cys,seq:gca)
     gene            complement(1675..1761)
                     /locus_tag="ACZPDN_01100"
     tRNA            complement(1675..1761)
                     /locus_tag="ACZPDN_01100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1725..1727),aa:Leu,seq:taa)
     gene            complement(1770..1843)
                     /locus_tag="ACZPDN_01105"
     tRNA            complement(1770..1843)
                     /locus_tag="ACZPDN_01105"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1809..1811),aa:Cys,seq:gca)
     gene            2011..2892
                     /locus_tag="ACZPDN_01110"
     CDS             2011..2892
                     /locus_tag="ACZPDN_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1297254.1"
                     /translation="MQHIIHQLQKEKKLRLLTSTIMIVFSAFMQCYIMNVFMAPCNLI
                     SGGFTGLALLINKVCALVNINFPTQVGIIALNVPAAILCYKHISPRFTFLSCVQFFLV
                     SFFISIFHFEPFFEDQILNVLFGGFLWGFSISLSLRAGGSTGGTDFIAQYVSSKIHRG
                     IWDYVFFYNCAMIVIFGYLCGWIYAGYSILFQFLSTKTISSLYQRYAQITVEFTTNDP
                     DPIIDAFMATCRHGMSVFECYGAYSHRKYYVCKAVISTYELRDVVDNVRKVDPKVLIN
                     TYNTVNFYGNFYQKPLE"
     gene            complement(2960..3670)
                     /locus_tag="ACZPDN_01115"
     CDS             complement(2960..3670)
                     /locus_tag="ACZPDN_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR1297255.1"
                     /translation="MFNNDSMNYYSTSTLKSALLKSYAWMAIALSITAIVSYGLYATN
                     ALMILLSTMPFLFMLFVIAPFILSIAFGVSMARSTSSTGMKVMLVLYAICMGVSLTSI
                     GYVYDVATIGTAFLVSAVYFISLAVIGTTSKKDFTSFGSLCMIGLFVLIITQCFMMLF
                     RVSMDVRLLSILTLLIFTGITVWDVQRMNRLLIQSDGSVVAQDKIAIFMALQLYLDFL
                     NIFLRILQLIGMGNRNNK"
     gene            complement(3673..5376)
                     /locus_tag="ACZPDN_01120"
     CDS             complement(3673..5376)
                     /locus_tag="ACZPDN_01120"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR1297256.1"
                     /translation="MQNKIEIFNNQVIINYNLAYPKSREVLLKSHTFAKFVQYFIEYQ
                     ETDNANMYAYLTKNGELSSKEAAYDFCHFLRLLSIFTCEELKDEYYLSDKDATLDVIE
                     EMYRTWRSLQRFGYMKSDNSTDFGINTLVAFDSASNDLFLRTYRLFEEKLMGRPNLVY
                     RQVQAGTNACFSVHTLDKVWADEYAALQDIPMIDTVMLRTPMILHPKSSKRTGMFTEI
                     DTQPMTYFKKGESNWFCYPCKVGALLCFVYFNSKYMSSALSMANLFELATKEERAQKP
                     DLVVIFGNDDGNDDTNFYYDKANDIWVGCISDNPRIEYFGYLKKMCLTLHNLAQMKKG
                     WLPIHGAFVNIYLKDGTKKGIMLMGDSGAGKSESIEALKAAAGDMIREVEVVFDDMGT
                     IHLEDGVPYGQGTEIGAFIRLDDLDPGTPYRDMDRSIFMAPENPNARMIQPAAPYRVV
                     VHNHKIDLFAYANNYTNEFGLKKLTVQEVKDTCKLGKRMALGTTQEVGISTTYFANPF
                     GPMQKQELCEPIIDTMFQALEDNGVFVGEIYTHLGFDKSNRKGLNIAAQQLLEFIQKK
                     D"
     gene            complement(5471..7165)
                     /locus_tag="ACZPDN_01125"
     CDS             complement(5471..7165)
                     /locus_tag="ACZPDN_01125"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1297257.1"
                     /translation="MKLSQSFFYTLRENAKDEDSVSSNLLVRAGMIKKCSNGIYMIMP
                     MGKKVLSKVENIIREEMDAKDAQELLMPALIPEDVYVQSGRREAFGSNMFSLSDRYNK
                     RYVLGPTHEELFAVASSMDGKSYKDFPYNLYQIQTKYRDETRPRYGLIRVREFIMKDA
                     YTFDVDEAGLDVAYNKMYDAYCRIMDRLGLEYKIVKADTGAMGGLLSEEYQALSSIGE
                     DVVVGCVGCDYSSNLEITEVVDTMEDSCEEELSMEVVDTPNARTIEEVAAFFGKKESD
                     FVKTLLYNVDGKVIAFCIPGDRELNETKALKLLGANEMEMASFEEVEKVTHARVGFAG
                     PVGIDCPVYMDRQIKHMKNFIVGANQSDRHIKNVNCKDFTPVEVADLCQVKEGDICPK
                     CGKKLTFQHGIEVGNLFKLGTKYSKSMNLYYTDANNQLQPVWMGSYGIGLERCMAAVA
                     DQHHDDFGIKWPIEIAPFRLAIVPVSLKDEEQVKIANALYEYCKEHKFEVILDDRNER
                     PGVKFKDMELIGIPYRITVGRGVKEGKLEWTDRYTGEKSEIAIEDVYTEIEKVFAM"
     gene            complement(7184..8386)
                     /locus_tag="ACZPDN_01130"
     CDS             complement(7184..8386)
                     /locus_tag="ACZPDN_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1297258.1"
                     /translation="MNKIELLAPAGNLEKAKIAILYGADAVYIGGKQFSLRSRASNFS
                     IDEIAELVKFANAHNSHVHVTVNMLPHESDLEGIEEYLKTLDLIGVHAIIVASPSIMM
                     LAKKLGCKFEVHVSTQHSSTNSSAVRFWKEKGMDRVVLARECQMNDIRSICEENILPI
                     EVFIHGGMCISFSGRCVLSNHMTLRDANRGGCAQSCRWKYHLMDGETELSDPTNLFSM
                     SSKDLQAVDYIEDFIHMGVASLKIEGRMKSAYYLATVVSSYRMLIDYIYEHGHADEKI
                     IERVKKEIAKAENRPTGPGFYNGLPGYKVQLYQDHDETVTQEYVAIVLDYDKESQLAT
                     LQVKNHFMPNQDLEIFGPHIQSTIVHVSDVYDSDKNVLEACNKPMQIVYTKWSGPIEV
                     DAMIRKIR"
     gene            complement(8379..9242)
                     /locus_tag="ACZPDN_01135"
     CDS             complement(8379..9242)
                     /locus_tag="ACZPDN_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1297259.1"
                     /translation="MFITTIHSIDSLEKLKRAKADAVIIGVEHYSIRSCITVKKEDLQ
                     LWKKKCKKLGLKLYINFLRLCMDEDLSGVREYFSIFKELDVDGIYYADEGLYYEAKQL
                     GIESKLIYQPETLVASSADVNFYMSLGIQSVSLAHELSLEEVVSIGTHNPDIEILVHG
                     YFSILYSRRPLVTNYLEAVNKPVCSLPCHYDLIEQTRQERMPIVQDETGTHIFSEMPI
                     NSLQEMWDFTDVGMHRFRIDSLFFDDDWTVEILDLYDLALQGIKITDDESSNRWYLEE
                     TIKKKEEQVNE"
     gene            complement(9495..10172)
                     /locus_tag="ACZPDN_01140"
     CDS             complement(9495..10172)
                     /locus_tag="ACZPDN_01140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1297260.1"
                     /translation="MKKLMFDCDDTLYDLSWPFRKCMEEFLPDVDVDMNQFYADYRKF
                     GDRIFDKLQQGKITIDESGVYRIEKVCEKYDIEFSHSKAVEFQSRYKYYQHHIEMDAP
                     LIDYFTNCEDELAILTNGEDAHQRMKLEVLHVFDYFKASHVFTSGQLGVAKPDVNAFK
                     KCMEAMQEDIHEWYYVGDNYINDMQGAKSAGMKTIHFNRHHQVSGPLADHVVYSAKEL
                     VEFIKKD"
     gene            complement(10214..11164)
                     /locus_tag="ACZPDN_01145"
     CDS             complement(10214..11164)
                     /locus_tag="ACZPDN_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR1297261.1"
                     /translation="MEKGKLNRITDVEGVLVGHKTICSGTHQTGVTFIRTAEDVFHQK
                     LACGCSILNGFGKTTGLPQIMELGTLESYICLTNTLNVGVVQQALVDIMVKENEDLTS
                     VNVVVGECNDGYLNSIRDCVVTKEDVYDAYSDCKKEFELGSVGAGRGMSCFEMSGGIG
                     SSSRVFEIDGNTYTLGVLVLSNFGLREQYLLDKVEGNQLPLEQEKGSIIMIIATDAPL
                     SDRQLHRVCNRMPVGLALTGSHMGNGSGDIAIAFSTANRFGDEKIHTLKQINDNKINV
                     VFDAAIESCKEAVHVSLIENEEVTGQSGHVRKCLNQVKIQ"
     gene            complement(11245..14793)
                     /gene="nifJ"
                     /locus_tag="ACZPDN_01150"
     CDS             complement(11245..14793)
                     /gene="nifJ"
                     /locus_tag="ACZPDN_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR1297262.1"
                     /translation="MPRAKQSMDGNTAAAHVAYAFTDVAAIYPITPSSPMADSIDQWS
                     AAGQKNIFGNQVVVSELQSEAGAAGAVHGSLAAGALTNTFTASQGLLLMIPNMYKIAA
                     EQLPCVFDVSARTVATHALNIFGDHSDVYACRQTGFAMLCETNPQEVMDLSPVAHLAA
                     LEGKVPFINFFDGFRTSHEIQKIEKWDYEDLKEMCPMEAVEEFRKHALNPERPTMRGS
                     HENGDIFFQHREACNTAYDELPAVVEKYMGKINEKLGTNYDLFNYYGDPEADRVIIAM
                     GSINDVAEEVIDYLTAKGEKVGLVKVRLYRPWSSKHLLKVLPKTAKKVAVLDRTKEPG
                     ALADPLYLDVATTLREAGLNDITLCGGRYGLGSKDTPPSSVFAVYTELLKDEPKPRFT
                     IGIVDDVTNLSLPEVKPAPNTSTPGTVECKFWGLGGDGTVGANKNSTKIIGDHTDKYI
                     QAYFQYDSKKTGGITISHLRFGDKPIRSPYYINQADFVACHNPSYVVNGYKMVQDVKP
                     GGVFMINCQWSDEELDKHMPAESKKYIADNNIQLYTINAIDKAIEIGMGKRTNTILQS
                     AFFKLANIMPIEEAVDYMKAAAKKSYSKKGDAVVEMNYKAIDAGVGATHKVEIPASWA
                     NPEADAPRPELSGRPATVKMVKDIMEPVNKMDGDSLPVSAFVGNIDGQWETGASAYEK
                     RGTAVTVPEWDAEKCIQCNQCAFVCSHATIRPFLLSEEEVKAAPAQIKLADVKPKATE
                     FKYTMSVSPLDCMGCGECITVCPTQAIKMVPQESQAEQQPVFDYLVANVSKKDSGFAD
                     DTVKGSQYNQPLLEFSGSCAGCAETSYARLITQLFGEHMYVSNATGCSSIWGGPASVS
                     PYTVNKDSLQGPAWANSLFEDNAEHGFGMQLGQKVLREQAIEKLGAMNESEKATPEFK
                     AAFEKFMETKENTRTNTEATKALLVEVEKAAEAGCEDAKAVLDKKEYLAKKSVWIFGG
                     DGWAYDIGFGGLDHVLASGENVNVMVFDTEMYSNTGGQASKASNIGEVCQFAAAGKEM
                     KKKSLAEIAMSYGYVYVAQIALGANPAQAVKCIAEAEAYPGPSLIIGYAPCELHGIAK
                     GGMNHCQDEMKKAVKSGYWNLFSFDPAKKEAGKNPFTLTSKEGDGTYQDFLHNESRYT
                     RLVKPFPERAEKLFAKSEEVAKERYEHLQRLVELYK"
     gene            complement(14953..16224)
                     /locus_tag="ACZPDN_01155"
     CDS             complement(14953..16224)
                     /locus_tag="ACZPDN_01155"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR1297263.1"
                     /translation="MITLDLNHAFLKEDVKAYQDQVRAIDEALQNGTCKGNDFIGWLN
                     WANNYDKDEFSRIKEVAAKVRENTEVFVVCGIGGSYLGARAAIEMMNGLYGDNKPEII
                     YMGNTFSSTYISQVMNYIKDKEVTVNVISKSGTTTETALAFRILKQFMEEKYGEDAKN
                     RIIATTDKARGTLKALADKEGYETFVIPDDIGGRFSVITPVGLLPIAVAGIDIDALMK
                     GLHDGMGEYGDADLEKNAAYRYAVARRILQNQGYDVELLVNYEPQMQMVAEWWKQLFG
                     ESEGKDGKGILPDSVCFSTDLHSLGQFVQEGKKVLFETNLYVEKPMIDLTFPNDEANE
                     DGMNYLAGKSVDWANKMAAKGTLQAHEETGGVPNILLTMPGMTSYDFGNMCMFFFKAI
                     AMTTLMNDSNPFNQPGVEVYKKNMFKLLGKE"
     gene            16315..16776
                     /locus_tag="ACZPDN_01160"
     CDS             16315..16776
                     /locus_tag="ACZPDN_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR1297264.1"
                     /translation="MIPSSLYPLVTALLSNVLAQVGKTVVYYYRTGKWDLHWVIASGG
                     FPSSHSSTVTALSLSIGIQEGFDSAIFAVTTIFSFIVMYDACHVRYYSGKNIELTQQL
                     VKDLREMTGLRFDDPIYQEKLKNVLGHKFVEVIGGFVVGLIVPLILCPLFL"
     gene            16794..17033
                     /locus_tag="ACZPDN_01165"
     CDS             16794..17033
                     /locus_tag="ACZPDN_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR1297265.1"
                     /translation="MDTVVDSKAVNDCLDKIRPYIQHDGGDIELLGVDEFNVVYVSFK
                     GACQGCMMASEDFSGGIKELLLEEVPNIRDVVLVG"
     gene            17075..18400
                     /locus_tag="ACZPDN_01170"
     CDS             17075..18400
                     /locus_tag="ACZPDN_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297266.1"
                     /translation="MYTKEQLRRLLIPLMFEQVLTALMGSVDTIMVTNIGSAAISAVS
                     LVDSLNILIINIFAAMATGGAIICAQYIGSNQKEKANQALKQLIFSITLISLLITLPC
                     ILFRIPLLTLIFGSVEKNVMDNSLSYLFITALSYPFIALYNAGAASFRTSQNSRLPMA
                     IAFGSNILNIIGNVFFIFTLSLGVAGAALSTLLSRIISAAIILILLKQPKQDLYIDSY
                     LSIRPEIETITKILKIGIPTGIENGMFQFGKLVIQSTVSTMGTTAIAAQAMVAMLESF
                     ACQASIGIGLGMMSVVGKCVGAKEYEQAKHYTILLTKYAWIACIICCAMIMFPIQQIT
                     SLAGMETESAKLAIWLTRIVFTYKMIFWTPSFLPGYGLRAAGDVKFSMITSTLSMWIC
                     RVVVTIFLVHTFHMGPLAVWIGMGADWTIRSFIFLARFKSNKWLEHKVI"
     gene            complement(18421..19119)
                     /locus_tag="ACZPDN_01175"
     CDS             complement(18421..19119)
                     /locus_tag="ACZPDN_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR1297267.1"
                     /translation="MTKIKNELISHTDLYVWTQINFAYHSNKIEGSHLSKNQTKQIFE
                     TNDLTMDLDDALEARNHFRLFDYMLKTVDEPLSKEMMIQMNMILKRGTSYEDNPAYNV
                     GGFKVRENGIGMINSFKTTKPANVETELDALLEKFQNKGVLSFEDIVEFHVCFERIHP
                     FGDGNGRTGRMIMFKQCLQNSHIPFVLLDRDRAFYLRGLKEWDFERNYLIDTLLTQQD
                     IYASVCEQLDFKKE"
     gene            complement(19116..19859)
                     /locus_tag="ACZPDN_01180"
     CDS             complement(19116..19859)
                     /locus_tag="ACZPDN_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIR1297268.1"
                     /translation="MQVQYIKEYSNCLDREMEYKIYGQGGKLCLAIPSQNGRFFEWED
                     RGMIHEVSKWIEANQLTIVTCDTLDLETWSYPYNTKVRMLKHELWFEYILSELIPSVQ
                     EKMDNYDKWMVTGASLGATHAANLIFRFPTQFDTLLALSGIYDTELFYGDYHDENTYH
                     NNPCAYMKNMSLDHPYMELYKQSKLIFCVGQGNWEQECVESLRQFSSILYDQQIEAWC
                     DFWGYDVYHDWPWWKVQLQYFMEQILKKV"
     gene            19969..21798
                     /gene="typA"
                     /locus_tag="ACZPDN_01185"
     CDS             19969..21798
                     /gene="typA"
                     /locus_tag="ACZPDN_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR1297269.1"
                     /translation="MSNKEKILNIAVIAHVDAGKSTLVDAFLRQSDVFRDNEEVVNCI
                     MDSNDLERERGITIYSKNCSVIHNGVKINIVDTPGHADFSSEVERIIRTVDTVILLVD
                     ASEGPMPQTRFVLSKALEIGLKPILLINKIDKKDQRAEEVVDEVYELFLDLNANDEQL
                     DFPILYGVAREGRVQYDLKTPSDNIDPLFDTILKHCDVYPDMDEEPLQMQVSALAYDE
                     YIGRLGIGRLYSGVLKQGQQYIRCNQSGLNAKESLSKLFVYKGLSRTSVQEAHSGDIV
                     VIAGMPDISIGDTICTADHIEPMEHIEIEEPTLSMNFHVNSSPFAGQSGKYVTSRNLK
                     ERLEKELEVNVGLKVEPTDSADCFKVSGRGELHISVLIENMRREGYELAVSKPEVILH
                     KDENGHKVEPIEEVVCLAPEEYSGTIINKLNLRKGVMQDMSEENGYVKIVYTAPTRGL
                     LGFRSEFINDTHGEGTLVRRISGYEPYKGEIAQRMEGAMISTETGEAMTYALWNLQER
                     GQLFISPQTPVYEGMIIGQSSKNIDLDVNPLKNKKLTAIRSSGRDEAMLLTPPKIFSL
                     EEALEWINDDELVEVTPDAIRIRKKGLTALDRRNLYRQKMNAQ"
     gene            complement(21850..22638)
                     /locus_tag="ACZPDN_01190"
     CDS             complement(21850..22638)
                     /locus_tag="ACZPDN_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297270.1"
                     /translation="MFKTGIKAIKNLTDYWYHLSNGSNNQVVNKSMKSLEFITASYHR
                     GISYDSSYKVGDFVWIEFGHNPKPELSFEHIGIIFMKQNHTYYVLPITTPKSHNRQHL
                     NAYHEVENPNGTKKFVLIKKVDFPFLKHDSLVKTSELRVVSEARLGNVLGHKELEDPK
                     IIDIWRIAHSFYFPSYDYKMNNLIKENSFFKMMIVASKINKTYTINEFTDLNDLIDID
                     TTKYTLEIGIPKLMSSTQYMVEIKMRDIFNQEITKSVECRLKTD"
     gene            complement(23109..>24707)
                     /locus_tag="ACZPDN_01195"
     CDS             complement(23109..>24707)
                     /locus_tag="ACZPDN_01195"
                     /inference="COORDINATES: protein motif:HMM:NF044955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 family protein"
                     /protein_id="HIR1297271.1"
                     /translation="NDEYEVDSDSGNRVIQIGSADYTIIGPHTYTLTYTIVIPEDYHS
                     DFDFIYYSVLGNNWDTTINHFSFDIQFEKALTEDEMSNIEVFSGSLGNQSNDLNVTPI
                     IISTSISGSANDIGAKQAITLFGKLRQGYFVGAKQTTSKIPFIFLGLAILFGLYSLIR
                     ALLLKKTHITPIVNFYPPKDMDPAMVGTIVDESVDQEDLMALIPYWASKGYLTISEKE
                     DDLVLTKQENHEPPILKHQKKIYNGLFKTKNSVKLSKLSSKFGKAMDDAKEALNDVFS
                     EDKKLSTIDHGFVTTILALVCMIVCILFNTRYSILDNLIETILATFSFVLMMVLNYSA
                     AATNVFNKNKLGVLKKILSIAFILILSYFVYRQCDSILSIVPFEFMMIGIVVVSLPIL
                     LISKFNVVSNYFKSVAPELLGFKDFVEKAEVPQLERLSLENPSYYYDVIPYAMVFGLS
                     EMWTKKFSTIPLAQPDWYDSYYSDPYSSYFYYRMLTRSMYEPIHENLMDYQTEQMKST
                     ADSMGSSFGGFSGGGAGGGGGGSW"
BASE COUNT         7862 a         4950 c         3782 g         8113 t
ORIGIN      
        1 ggaggaacta cctttgaatt ttatagacat tattttagat acaatacacg atacatgggc
       61 tatgcttcca ctattgtata ttacttattg tatattagaa gttttcgaaa gaaagaatac
      121 gcaagacgat aaactcttct ttggactaca aagacttgga cccattctag gcgcaatagt
      181 cggattgatt ccacaatgtg gatttagtat tctagccgct atgttgtttg ttcaaaataa
      241 cattacccta ggaactttag tcagtgttat gattgcgaca agcgatgaag ccattccaat
      301 ccttattagt aatccaaaga tgtattcatc attgcttggt attttaggat taaaattagt
      361 tatcgcaatt cttgttggat tatttaccga tcatgtatta tttcgcaaac aaaaaatcct
      421 ccgcttcgaa gatatggaag aagaggatta tgatgaagac gaatatgagg aagaagaaaa
      481 ttcaggatca agctgtccat gttgttatcc tgaatatcca ttatggatca gtgccttatt
      541 aagatcttta aaaatctatg tattcatctt tattactagt tttgtattaa cactattaat
      601 ggaatgtatt ggagaaaaaa cattatctat gatcttactt caagataatt tattccaacc
      661 aatcatcgcc gcactattcg gctttattcc taactgtgca gctacagttg ttctaacaca
      721 actctataca gctggtgaat tatcctttgg atcgctactt gcaggacttg tcacaaatgc
      781 aggactgggt ttaattgtga tgattcgtta tgaagcaaga aagaaagaca ttttaaaaac
      841 catttccatc ctatttataa caggtgttat cgtaggcatt ggatttatgc tattatagcc
      901 actattgtgg ctattttttt atctttgtgg aaagatccag atttcaaaat gttcaatgtc
      961 ataccaaaac aaaagaataa gtatggcaat cgcaaataac aaaatataaa agataataga
     1021 attcagcttt tgcgaataat tctgttcatt caattctgga tgctcctcta attcagccaa
     1081 tcgcttcttt gctattttat caagccaact catagttacc tctacttaga ctaatattgg
     1141 aaaatgaaat ggaaatatat caatttccgg atgattccat tgataccaaa gcgctaaaac
     1201 taatgcagct agtaagataa caattacata gaatttaata tctttcatca ttttatttcc
     1261 ctgttcttca aaatcatttg aattcaattc tggacgctca tccaattctt tgattcgttt
     1321 tttcgttgtt ctttcaatcc agctcataga atgcacctct cttttattat attttaatca
     1381 ttatgtttat atacaaatat ggtaaacttg gttattgtgc tggccaaata agtacgaaaa
     1441 aagctagaaa caaatgtttc tagctagctt ttccaaaaaa tggtggggac ggtgggactt
     1501 gaacccacac gggatttctc ccaacggatt ttaagtccgt tgcgtctgcc tattccgcca
     1561 cgaccccatg taattggagg ttccgatcag atttgaactg atgatcacag agttgcagtc
     1621 tattgcctta ccacttggct acggaaccgt aacttgttga tttttaataa acgatggtgg
     1681 ggacggtggg acttgaaccc acacgggatt tctcccaacg gattttaagt ccgttgcgtc
     1741 tgcctattcc gccacgaccc catcatttat ggaggttccg atcagatttg aactgatgat
     1801 cacagagttg cagtctattg ccttaccact tggctacgga accgtttcgt tgactgccca
     1861 tatataatac ctcaacgcat aataaaaagc aagaaaaaaa tttatttttt ttacattttt
     1921 ttaaaaacca tacatttttc atttttggtt gaacttattt gacaaatgac ctattataat
     1981 gtggtcaata aacaagaggt attttatatt atgcaacaca ttattcacca gcttcaaaaa
     2041 gaaaagaagc taagactatt aacgtcgacc ataatgattg tatttagtgc ttttatgcaa
     2101 tgttatatca tgaatgtatt tatggcgccg tgcaatttga tctcaggagg atttacagga
     2161 cttgctctat taatcaataa agtctgtgct ctcgtaaaca ttaattttcc tactcaagtt
     2221 ggaatcatcg ctttaaatgt tccagcagcc attctatgct acaaacacat ctcacccaga
     2281 ttcacatttt tgagttgtgt gcaattcttt ctagtatcct tctttatttc tatcttccat
     2341 tttgagccct tctttgaaga tcaaatctta aacgttctat ttggaggctt cttatggggc
     2401 ttctccattt ccctatccct aagagctgga ggatctactg gaggaacaga tttcattgcc
     2461 caatacgttt ctagtaagat tcatagaggc atatgggact atgtattctt ctataactgc
     2521 gctatgattg ttatctttgg atatttatgt ggatggatct atgcaggata ttccattcta
     2581 ttccaattct tatctacaaa gacaatctca tctttatatc aacgctatgc acagattacc
     2641 gttgaattta cgacaaacga tccagaccca attatcgatg cctttatggc cacttgtcgt
     2701 catggtatga gcgtatttga atgttatggt gcatatagcc atcgtaagta ttatgtatgt
     2761 aaagccgtta tttctactta tgaattaaga gatgttgtcg ataacgtaag aaaagttgat
     2821 cctaaagtat tgatcaacac atacaatacc gtaaacttct acggaaactt ctatcaaaaa
     2881 cctttggagt agtattacac tactccattt ttatttacaa taaaaggaaa tgtatcatta
     2941 aaatacattt ccttttcatt tacttattat tacgatttcc cattccaatc aattgtaaga
     3001 ttcttaagaa gatattcaag aaatccaaat acaattgtaa tgccatgaag atggcgatct
     3061 tatcttgtgc tacgactgaa ccatccgatt gaatcaacaa tcgattcatt ctttgaacat
     3121 cccaaacggt gatgcctgta aaaattaata gagtaaggat agaaagtaag cgaacatcca
     3181 tactgactct aaatagcatc ataaagcatt gtgtaataat caatacaaaa agtccaatca
     3241 tacataatga gccaaagctt gtaaaatctt ttttagatgt tgtaccaata acagctaaac
     3301 taataaagta aactgcactg actagaaaag cagtaccaat tgtagcgaca tcatatacat
     3361 agccaatcga agtaagtgaa acacccatac agatggcata aagcactaac ataaccttca
     3421 tacctgtact tgatgtagat cttgccatag atacaccaaa ggcaatcgac aagataaatg
     3481 gtgcaatcac aaacaacata aatagaaatg gcattgtact taataaaatc attaaagcat
     3541 ttgtagcata tagaccataa gatacaattg ccgtaataga taaggcaata gccatccatg
     3601 cataagactt caacaaagca ctttttaaag tgcttgttga gtaataattc atagaatcgt
     3661 tattaaacat tattaatcct tcttctggat aaattctaat aattgctgag ctgcaatatt
     3721 taaaccttta cggtttgatt tatcgaatcc taagtgcgta tagatttcac ccacaaatac
     3781 tccattgtct tctagagcct ggaacattgt gtcgatgatt ggttcacaaa gttcttgttt
     3841 ttgcatagga ccaaatgggt ttgcgaaata tgtagttgaa ataccaactt cttgtgttgt
     3901 acccaaagcc atacgcttac ctaacttaca agtatctttt acttcttgta cagttaattt
     3961 ctttagacca aattcattag tgtagttgtt tgcgtatgca aataagtcaa ttttatggtt
     4021 gtgaacaaca acacgatatg gagctgctgg ctgaatcata cgtgcgtttg gattttctgg
     4081 agccatgaaa attgaacggt ccatgtcacg atatggagta cctggatcca agtcatctag
     4141 acgaatgaag gcaccaattt cagtaccctg accatatgga acaccatctt ctaagtgaat
     4201 tgttcccata tcatcaaata caacttcaac ttcacgaatc atatctccgg ctgccgcttt
     4261 taatgcttca atcgattcag atttaccagc accactatca cccatcaaca tgataccttt
     4321 ctttgttcca tcctttaaat agatgtttac gaaagcacca tggattggta accatccctt
     4381 tttcatttgt gccaagttgt gtaatgttaa acacattttc tttaaatatc caaagtattc
     4441 gatacgagga ttatcactga tacatcctac ccaaatatca ttggctttat cataatagaa
     4501 gtttgtatcg tcatttccat catcgttacc aaaaataaca actaaatctg gtttttgcgc
     4561 cctttcttct ttagtagcta attcaaataa gttagccata ctcaatgcac tagacatata
     4621 ttttgagttg aagtatacga aacataataa agctccaact ttacaaggat aacagaacca
     4681 gtttgattct ccctttttga aatatgtcat aggttgtgta tcaatttcag taaacatacc
     4741 tgtacgttta cttgattttg gatgcaaaat cattggagtt cttaacataa ctgtatcgat
     4801 cattggaata tcttgtaaag ctgcatattc atcagcccat actttatcca aagtgtgaac
     4861 agagaaacat gcatttgttc cagcttgaac ctgacgatac actaagtttg gtcttcccat
     4921 taatttttct tcaaacaaac gatatgtacg aaggaataaa tcatttgaag ctgagtcaaa
     4981 tgccactaat gtattaatac caaaatccgt tgaattatca ctcttcatat atccaaaacg
     5041 ttgtaaagaa cgccaagtac gatacatctc ttcaataaca tctaaagttg catctttatc
     5101 tgaaagataa tattcatctt ttaattcttc acaagtaaag attgacaata aacgtaagaa
     5161 atgacagaaa tcataagctg cttctttaga agataattca ccattttttg tcaaataagc
     5221 atacatattc gcattatctg tttcctgata ttcaatgaaa tattgaacga attttgcgaa
     5281 tgtatgagac tttaataaaa cctcacgact ctttggatac gccaaattat agttgataat
     5341 aacctgattg ttaaaaattt caattttatt ctgcataatt acactccttt aactatgact
     5401 aatatacatg aaaaaccttt gaaagtaaat gtgattctaa attttttgtt tatatctcaa
     5461 gtacaagcgt ttacatggcg aaaacttttt cgatttcagt atatacatcc tcaatcgcaa
     5521 tttcactctt ttcacctgta taacgatctg tccactctaa ttttccttct ttgacaccac
     5581 gacctaccgt aatacgataa ggaataccaa tcaattccat atctttgaat ttaacacctg
     5641 gacgctcatt acgatcatct aaaattacct cgaacttatg ttctttacaa tattcatata
     5701 aagcattcgc aatcttaact tgttcttcgt cctttaaaga tactggaaca atcgcaagtc
     5761 taaatggtgc aatttcaatt ggccatttaa taccaaaatc atcatgatgc tgatctgcaa
     5821 cggcagccat acatctttct agaccaatac cataacttcc catccaaact ggctgtaatt
     5881 ggttgttggc atctgtatag taaagattca tactcttcga atatttcgta cccaatttaa
     5941 ataagttacc aacttcaata ccgtgttgga aagttaattt cttaccacac ttaggacaaa
     6001 tatccccttc ttttacctga caaagatctg ccacttcaac aggcgtaaaa tctttacagt
     6061 tcacattctt aatatgacga tccgattggt ttgcccctac aatgaagttt ttcatatgtt
     6121 tgatttgacg atccatataa actggacaat caataccgac tggacctgca aaaccaacac
     6181 gagcatgtgt tactttttca acttcttcaa agctagccat ttccatttca ttcgcaccca
     6241 ataatttcaa tgcctttgtc tcattcaatt cgcgatcacc aggaatacag aatgcgatga
     6301 cttttccatc tacattataa agtaatgttt tgacaaaatc actttccttc ttaccaaaga
     6361 aagcagccac ttcttcaatt gttcttgcgt ttggcgtatc cacaacttcc atagaaagtt
     6421 cttcttcaca agaatcttcc attgtgtcta ctacttctgt gatttctaag ttgctcgaat
     6481 agtcacaacc aacacagcca acaacaacat cttctccaat acttgataga gcctgatatt
     6541 cttccgataa taaaccaccc atagctcccg tatccgcttt aacgatttta tattccaatc
     6601 ctaaacgatc catgatacga caatatgcat catacatttt attgtatgct acatccaatc
     6661 cagcttcatc cacatcaaat gtatacgcat ccttcatgat aaattcacga acacgaatca
     6721 atccataacg aggacgagtt tcatcacgat atttcgtttg aatctgatat aagttatatg
     6781 ggaaatcctt ataagatttt ccatccatac tactcgctac cgcaaacaat tcttcatgag
     6841 taggacctaa cacataacgc ttattataac gatcactcaa tgaaaacata tttgaaccaa
     6901 atgcttcacg acgacccgat tgtacatata catcttccgg aatcaatgca ggcatcaata
     6961 actcttgcgc atctttcgca tccatttctt cacgaataat attttcaact ttagaaagaa
     7021 ccttctttcc cataggcatg atcatataaa ttccgtttga acatttcttg atcattcctg
     7081 cacgcaccaa taaattactt gatacgctat cttcatcttt cgcattttct cttaatgtat
     7141 aaaagaaact ctgacttaat ttcatcttta cttacctctt ccactatcta atcttacgaa
     7201 tcattgcatc gacttcaata ggcccagacc acttcgtata tacgatctgc atcggcttat
     7261 tacaagcctc taatacattc ttgtcactat catatacatc tgaaacatgt acgatcgtac
     7321 tttgaatgtg aggaccaaat atttctaaat cttgatttgg cataaaatga ttcttcacct
     7381 gtaaagttgc caactgactt tccttgtcat aatctagcac aatagctaca tattcctgtg
     7441 ttaccgtttc atcatgatct tgatacaact gcaccttata tccaggaagc ccattataga
     7501 agcccggccc tgtcggtcga ttttctgcct ttgcgatttc cttttttaca cgctcaatga
     7561 ttttttcatc cgcatgccca tgctcataaa tataatcaat caacatacga tagcttgata
     7621 caaccgtagc taaataataa gcacttttca tacgcccttc aattttcaaa gacgctacac
     7681 ccatatggat gaaatcctca atataatcta ctgcttgtaa atcttttgaa gacatggaga
     7741 ataaatttgt tggatcacta agttcggttt caccatccat caaatgatat ttccaacgac
     7801 aactttgtgc acatccacca cggtttgcat cacgaagtgt catatgatta ctcaatacac
     7861 aacgtcctga aaaagaaata cacatacctc catgaataaa gacttcaatt ggtaatatat
     7921 tctcttcaca aattgaacga atatcattca tttgacattc tctagcaaga acaacacggt
     7981 ccatgccctt ttctttccaa aatcttacag cacttgaatt tgtagaagaa tgctgtgtag
     8041 atacatgcac ttcaaacttg caaccaagct ttttagccaa catcataatg gatggacttg
     8101 ccacaataat cgcatgcaca ccaatcaaat ccaacgtttt taaatactct tcaatacctt
     8161 caagatccga ttcatgtgga agcatattta cagttacatg cacatgacta ttatgtgcat
     8221 tcgcaaattt gactaattct gcaatttcat caatggaaaa gttacttgca cgcgaacgca
     8281 aactaaactg cttaccacca atatatacgg catccgcccc atataaaatc gcaatctttg
     8341 ccttttctaa atttccagca ggagctaata attcaatttt attcatttac ttgctcctcc
     8401 tttttcttaa ttgtctcttc taaataccaa cgattacttg attcatcatc cgtaatttta
     8461 atgccttgta aagccaaatc atataaatcc aagatttcaa ctgtccaatc atcatcaaag
     8521 aacaaagaat caatacggaa acgatgcatt cctacatctg taaaatccca catttcctgt
     8581 aatgagttga ttggcatttc actaaagata tgcgtacccg tttcatcttg tacaattggc
     8641 atacgctctt gtctagtttg ttcaatcaaa tcataatgac atggtaatga acataccggt
     8701 ttattcactg cttctaaata gttagtgacc aaaggtcttc ttgaataaag aattgagaaa
     8761 tagccatgca ctaaaatttc tatatccgga ttatgagtac caatacttac tacttcttca
     8821 agacttaact catgtgctaa agatacagat tgaatcccca aagacatata gaaatttaca
     8881 tctgcacttg aagccaccaa tgtttcaggt tgataaatca atttggattc aatccccaat
     8941 tgttttgcct cataatatag accttcatcc gcataataaa taccatccac atctaattct
     9001 ttaaaaatag aaaagtattc acgaacaccc gataaatctt catccataca taatcttaag
     9061 aagttgatat ataattttag gccaagcttt ttacacttct tcttccaaag ctgtaaatcc
     9121 tcttttttta ccgtaataca cgaacgaatg gaataatgtt caacaccaat gattacggca
     9181 tcggccttcg ccctttttaa tttttctaat gaatcaatag aatgaattgt tgttataaac
     9241 ataattactc cttattaaaa catacagatt tattttacca caaatgtgat tttatgccta
     9301 cttaacctcc tatttaaatc ggacttaatt tcatatatac tttaaatatg aacaaacaaa
     9361 cacttaatga gagatttgaa gattatactg aagacaccaa acaagaagaa atatggtcgg
     9421 ataaaatcat tggagaagaa gaattgtaga aaaaaagaat gccattacga cattcttaac
     9481 atggctagtt caatctagtc ttttttaatg aattcaacca attctttagc cgaatatacc
     9541 acatgatctg ccaaaggacc acttacttga tgatgccgat taaaatgaat cgtcttcatg
     9601 cctgcacttt tagcaccttg catatcgttg atatagttat cccctacata ataccattca
     9661 tgaatatctt cctgcatcgc ttccatacac tttttaaatg cattcacatc cggttttgcg
     9721 acacccaact gtccacttgt aaagacatga gaggctttaa aataatcaaa gacatgaagt
     9781 acttctaatt tcattctttg atgcgcgtcc tctccatttg ttagaatcgc cagttcatct
     9841 tcacaattcg tgaaataatc aatcaatggt gcatccattt caatatgatg ctgataatat
     9901 ttatagcgag actgaaactc tacagctttt gaatgagaaa attcaatatc atacttttca
     9961 caaacttttt cgatacgata aacgccagat tcatcaatcg taatctttcc ttgttgaagt
    10021 ttgtcaaaga ttctgtctcc aaacttacga taatccgcat aaaattgatt catatccaca
    10081 tctacatcgg gaagaaattc ttccatgcat ttacgaaatg gccaagataa atcataaagg
    10141 gtatcatcac aatcaaacat taatttcttc atataatcac cgaatgaaat ataacataaa
    10201 agtgaagatt tcactactga atcttcactt gatttaaaca tttacgaaca tgacccgact
    10261 gtccagttac ctcttcattt tcaatcaaag atacatgcac agcttcttta caagattcaa
    10321 tggctgcatc aaatacaaca ttgattttat tgtcattgat ttgttttaaa gtatggatct
    10381 tttcatcccc aaaacgatta gctgtactaa aggcaatcgc aatatcacct gaaccattgc
    10441 ccatatgtga tcctgttaaa gctaaaccga ctggcattct gttacaaact ctatgaagct
    10501 gacgatcact taatggagca tccgtcgcaa taatcattat aatcgaacct ttttcttgtt
    10561 ctaaaggcaa ttgattcccc tctactttat ctaacaaata ttgttctctt aagccaaaat
    10621 tagaaagtac aagtacacct agagtatatg tatttccatc aatctcaaag actcttgaac
    10681 ttgatccaat accacctgac atttcaaaac aagacatgcc tcgacctgct ccaacactgc
    10741 caagttcaaa ctctttttta caatcactat atgcatcata aacatcttct tttgtaacaa
    10801 cacaatcacg aatcgaattt agatatccat cgttacattc tccaacgaca acatttacac
    10861 ttgttaagtc ctcattttct ttaaccatga tatccactaa ggcttgttga acaacgccca
    10921 catttaaagt gtttgtcaaa caaatatagc tttctagtgt tcccaactcc ataatttgtg
    10981 gtaagcctgt cgtctttcca aaaccattta ggatactaca accacaagct agcttttgat
    11041 gaaacacatc ttcagccgta cgaataaatg taacacctgt ttgatgagtt cctgaacaga
    11101 ttgtcttatg tccaaccaat acaccttcta catctgtaat ccgatttaat tttccttttt
    11161 ccataattaa acacctctta aaaccattat agtgaaattc cacaaaaaag aagccgaaat
    11221 caatcagctt cttcatttat tatcttactt gtaaagttct actaaacgtt gtaagtgttc
    11281 gtaacgttct ttagctactt cttctgattt agcaaataat ttttctgctc tttcagggaa
    11341 tggtttcact aaacgagtgt aacgagattc gttgtgtaag aagtcttggt aagttccatc
    11401 tccttcttta gaagttaatg tgaatgggtt ctttcctgct tctttcttag ctggatcaaa
    11461 tgagaataag ttccagtatc cagatttaac ggctttcttc atttcatctt gacagtggtt
    11521 cattccaccc ttagcaatac cgtgtaattc acatggtgca taaccgatga ttaaagatgg
    11581 tcctggataa gcttctgctt cagcaataca tttaactgct tgtgcagggt tagctccaag
    11641 agcgatttgt gctacataaa cgtatccgta gctcattgcg atttctgcta aagatttttt
    11701 cttcatttct ttaccagctg cagcgaactg acaaacttca ccgatgttag aagctttaga
    11761 agcctgtcca ccagtgtttg aatacatttc tgtatcgaat accataacgt ttacgttttc
    11821 acctgatgca agaacgtggt ctaatccacc gaatccgata tcataagccc atccgtctcc
    11881 accgaagatc caaacagatt tcttagctaa gtattctttc ttgtctaata cagctttagc
    11941 atcttcacat ccagcttcag cagctttttc tacttcaact aataatgctt ttgttgcttc
    12001 tgtgttagta cgagtatttt ctttagtttc catgaatttt tcgaatgcag ccttgaattc
    12061 aggagttgct ttttcgcttt cgttcatagc acctaatttt tcgatagctt gttcacgtaa
    12121 tactttttgt cctaattgca taccgaatcc gtgttcagca ttgtcttcga acaatgagtt
    12181 agcccatgct ggtccttgta agctatcttt gtttactgta tatggagata cagatgctgg
    12241 accaccccaa atagaagaac atccagtcgc atttgataca tacatgtgtt caccgaataa
    12301 ttgagtaatt aaacgagcat atgatgtttc agcacatcca gcacatgatc ctgagaactc
    12361 taacaatggt tggttgtatt gagaaccctt aacagtatca tcagcaaatc cagaatcttt
    12421 cttagaaacg ttagctacta agtaatcgaa tactggttgt tgttcagctt gagattcttg
    12481 aggtaccatc ttaatagctt gtgttggaca tacagtgata cattcaccac atcccataca
    12541 atctaatgga gatacgctca ttgtatattt aaattctgta gcttttggtt taacatcagc
    12601 taatttgatt tgtgcaggag cagccttcac ttcttcttcg ctcaataaga atggacgaat
    12661 agtagcgtga gaacatacga atgcacattg gttacattga atacattttt cagcatccca
    12721 ttcaggaact gtaacagctg taccacgttt ttcgtaagca ctagcaccag tttcccattg
    12781 accatcgatg ttaccaacga atgcagatac aggtaatgaa tctccatcca ttttgttaac
    12841 tggttccatg atgtctttaa ccatcttaac tgttgcagga cgtcctgata attcaggtct
    12901 tggtgcatca gcttctgggt tagcccaaga tgcaggaatt tcaactttgt gagttgcacc
    12961 tacaccagca tcgatagctt tgtagttcat ttctacaact gcatcaccct tcttagagta
    13021 agatttctta gcagctgctt tcatgtaatc tacggcttct tcaattggca taatgttagc
    13081 taatttgaag aatgctgatt gtaagattgt gtttgtacgt ttacccatac cgatttcgat
    13141 agctttatcg atggcattga ttgtgtacaa ttggatattg ttgtctgcga tatatttttt
    13201 agattcagca ggcatgtgtt tgtctaattc ttcgtctgac cattggcagt tgatcatgaa
    13261 tacaccacca ggtttaacgt cttgaaccat cttgtatcca tttactacat atgaagggtt
    13321 gtgacaagct acaaagtcag cttggttaat gtagtatggg ctacggattg gcttgtctcc
    13381 aaaacgcaag tgagaaattg tgataccacc tgttttctta gagtcatatt ggaagtaagc
    13441 ctgaatgtat ttgtcagtgt ggtcaccgat gatcttagta gagttcttgt tagcacctac
    13501 agtaccgtct cctccaaggc cccagaattt acattcaaca gttcctggag ttgaagtatt
    13561 aggagctggt ttaacttctg gcaatgataa gtttgtaacg tcatccacga taccaattgt
    13621 aaaacgaggt tttggttcgt cttttaacaa ttctgtgtaa acagcgaata cacttgaagg
    13681 tggagtatcc ttagatccta aaccataacg accaccgcaa agagtgatgt catttaatcc
    13741 tgcttcacga agtgttgtag ctacatctaa gtataatggg tctgctagag cacctggttc
    13801 tttagtacgg tcaagtactg caactttctt agctgtctta ggaagaactt ttaataagtg
    13861 tttgcttgac caaggacgat ataaacgaac cttaactaaa ccaacttttt ctccttttgc
    13921 agttaagtag tcaataactt cttcagcaac gtcattgata gaacccatag cgatgattac
    13981 acgatccgct tcaggatctc catagtagtt gaacaagtca tagtttgttc ctaatttttc
    14041 attgatttta cccatgtatt tttcaactac agcaggtaat tcatcatatg cagtgttaca
    14101 tgcttcacgg tgttggaaga agatatctcc attttcgtga gaaccacgca tagttggacg
    14161 ttctggattt aaagcgtgtt tacggaattc ctcaacagct tccattggac acatttcttt
    14221 taaatcttca tagtcccatt tttcgatttt ttgaatttcg tgagaagtac ggaatccatc
    14281 aaagaagtta atgaatggta ctttaccttc taaagcagct aagtgagcta ctggagataa
    14341 gtccataact tcttgtgggt tagtttcaca taacatagcg aaacctgttt gacgacaagc
    14401 atatacgtct gagtgatcac caaagatgtt caatgcatga gttgcaactg tacgtgcaga
    14461 aacatcgaat acacaaggca attgttcagc agcaatcttg tacatgtttg gaatcatcaa
    14521 taataaacct tgagatgcag tgaaagtatt agtcaatgca ccagcagcta atgatccatg
    14581 aactgcacca gcagcacctg cttctgattg taattcactt acaactactt gatttccaaa
    14641 aatatttttt tgtccagctg cagaccattg gtcaatagag tctgccatag gactagatgg
    14701 agtaatagga taaattgcag caacgtctgt aaaagcatac gcaacgtgag ccgcagccgt
    14761 atttccatcc atagactgtt ttgctctagg cattaatatt tcctccttta tagatattca
    14821 ataccatagt ttattatacg agcttcatgt caaattttaa aggtaaaatt cactgtagag
    14881 gttagtctta tctaatttgt aagcgtataa aaaaagagac gtaaacactt gcttacatct
    14941 ctattttcat tattattctt tacctaataa tttgaacatg ttctttttat aaacttcaac
    15001 accaggctga ttgaatgggt ttgaatcatt catcaaagtt gtcattgcaa tggccttgaa
    15061 gaagaacata cacatattac caaagtcata agaagtcata cctggcatag ttaataagat
    15121 atttggaact ccaccagttt cttcatgtgc ctgcaatgtt cctttggcag ccattttatt
    15181 agcccaatca acactcttac cagctaagta gttcattcca tcttcgtttg cttcatcatt
    15241 agggaatgtt aagtcaatca ttggtttttc aacatataag tttgtttcaa acaatacttt
    15301 cttaccttcc tgaacaaatt gtcctaaaga gtgtaagtct gttgagaagc atactgaatc
    15361 cggtaaaata ccctttccat cttttccttc actttcacca aacaattgtt tccaccattc
    15421 tgcaaccatc tgcatttgag gttcatagtt tactaataat tcaacatcat atccttgatt
    15481 ttgaagaata cgacgagcta ctgcataacg gtaagctgca ttcttttcaa gatctgcatc
    15541 tccatattca cccataccat catgtaaacc tttcatcaaa gcatcgatat cgattccagc
    15601 tactgcaatt ggcaataatc caactggtgt aattactgag aaacgaccac caatatcatc
    15661 aggaattaca aatgtttcat atccttcttt atccgctaaa gctttcaatg ttcctctagc
    15721 cttgtctgta gtggcaataa tacgattttt cgcatcttca ccatattttt cttccataaa
    15781 ttgtttcaag atacggaatg ccaatgctgt ttcagttgta gttccagatt tagaaataac
    15841 atttacagta acttccttat ctttaatata gttcataact tgtgaaatat aagtagatga
    15901 gaaagtgttt cccatataaa taatttcagg cttgttgtca ccatataaac cattcatcat
    15961 ttcaatggca gcacgagcac ctaaataaga tcctccaata ccacatacaa cgaatacttc
    16021 tgtgttttca cgtacttttg cggcaacctc tttaatgcga ctaaattcat ctttatcata
    16081 attgttagcc cagtttagcc aaccaataaa gtcatttcct ttgcatgtgc cattttgtaa
    16141 agcttcatca attgcgcgaa cttgatcttg ataagccttt acatcttctt ttaagaaagc
    16201 atgatttaaa tctaacgtaa tcatatttat tttccctcca tagctatcct aaggatgatt
    16261 ataccacgat ttaacattgt gtgatataat gatttttgtt aggagtgtga gtttatgata
    16321 ccatcgtcct tatatccgct agttacagct cttttgagca atgtacttgc acaagtcgga
    16381 aaaacggtcg tatattatta tagaactggc aaatgggact tacactgggt catcgcaagt
    16441 ggcggctttc caagcagtca ctcatcaacc gtaacggccc tttccctttc cataggaatt
    16501 caggaaggct ttgattcagc catatttgcc gtaaccacta tcttttcctt tattgtcatg
    16561 tatgatgcat gtcacgtccg ctattattca ggtaaaaaca ttgaattaac gcaacaatta
    16621 gtcaaagatt tacgtgaaat gacaggactt cgttttgatg atccaatcta tcaagaaaaa
    16681 ttaaaaaatg ttcttggtca taaatttgtc gaagtcattg gaggatttgt ggtaggactt
    16741 attgtcccat tgattttatg tccactattt ttataagtta ggagtgttat tttatggata
    16801 ctgtagtaga tagtaaagcc gttaatgatt gtttggataa gattcgtcca tatatccaac
    16861 atgacggagg cgacattgaa ctattaggag ttgatgaatt caacgttgtc tatgtttctt
    16921 tcaaaggtgc gtgccaagga tgcatgatgg cctctgaaga tttctcaggt ggaatcaaag
    16981 aattattact tgaagaagta ccaaacatcc gtgatgtagt ccttgtaggt taagatatcg
    17041 aaagatatct ttttattttt aaaggagttt taccatgtac accaaagaac aattaagaag
    17101 gcttttgatt ccattaatgt tcgaacaagt tttaaccgca ttaatgggat ctgttgacac
    17161 cattatggtt accaatatag gttcggccgc tatttccgcc gtctcccttg tcgattcatt
    17221 aaatattttg atcatcaata tatttgccgc catggcaact ggaggcgcca ttatctgcgc
    17281 ccaatacata ggatccaacc aaaaagaaaa ggccaaccaa gccttgaaac aattgatttt
    17341 ttccataacc cttatttctc ttctgattac cctaccctgc attctattca gaataccact
    17401 acttaccctt atctttggat ccgtagaaaa aaatgtgatg gacaattctt taagctatct
    17461 attcatcacg gcactatcct accccttcat cgcactatac aatgcaggtg ctgcaagctt
    17521 tcgaacaagt caaaattcaa gacttcccat ggccattgcg tttggctcta acatattaaa
    17581 cattatagga aatgtattct tcatctttac cctaagctta ggcgtagccg gagctgccct
    17641 atccacatta ctatcaagaa tcatcagtgc cgctattatc ctgattcttt taaaacagcc
    17701 caaacaagat ttatatattg attcctattt aagtatccgt ccagaaattg aaacaatcac
    17761 aaaaatatta aaaataggta ttccaaccgg cattgaaaac gggatgtttc aatttggaaa
    17821 actcgtcatt caatccactg tatctacaat gggaacaacc gccattgccg cacaagccat
    17881 ggtagccatg cttgaaagtt ttgcctgtca agcaagcatc ggtattggac ttggtatgat
    17941 gagcgtagtt ggaaaatgtg taggtgctaa agaatatgaa caagcgaaac actatacaat
    18001 cttactcaca aaatatgcat ggatagcctg cattatatgt tgtgctatga tcatgtttcc
    18061 aattcaacaa atcacaagtc ttgctggaat ggagacagaa agcgcaaaac tagccatttg
    18121 gctaacacgc attgtcttca cttataagat gattttctgg acaccctcat tcttacccgg
    18181 ctatggcctg agagcggctg gcgatgtaaa atttagtatg atcacatcca cactatctat
    18241 gtggatttgc cgagttgtag ttactatctt tttagttcat acttttcata tgggcccctt
    18301 agccgtatgg attggtatgg gggccgattg gacaattcgc tcattcatat tcttagcccg
    18361 ttttaaatcg aataaatggc tagaacataa ggtcatctaa cacaaaagga gtagttcacg
    18421 ctactccttt ttaaaatcta attgttcaca gacagatgca taaatatctt gttgtgttaa
    18481 aagagtgtct attaaataat ttctttcaaa atcccattct tttaagccac gcagataaaa
    18541 cgcacgatct cgatctaata gtacaaatgg aatatgacta ttctgtagac attgtttaaa
    18601 cataatcatt cgtccggtac gtccatttcc atctccaaac ggatgtattc tttcaaaaca
    18661 aacatgaaat tctacaatat cttcaaatga tagaacccct ttattttgaa acttttctaa
    18721 caaagcatct aattccgttt caacattcgc tggttttgtc gttttaaaag aatttatcat
    18781 tccaatccca ttttccctta ctttaaaacc acctacatta taggctggat tgtcctcata
    18841 agacgtaccc cttttcaaaa tcatattcat ttgaatcatc atttcttttg ataatggttc
    18901 atccactgtc ttcaacatat aatcaaacaa ccgaaaatga tttcgtgctt ctagtgcatc
    18961 atccaaatcc attgttaaat cattcgtttc aaatatttgt tttgtttgat tcttagataa
    19021 atgagaaccc tctattttgt tagaatgata cgcaaagtta atttgtgtcc acacatacaa
    19081 atccgtatgt gaaatcaatt catttttaat ttttgtcata ctttctttaa tatttgttcc
    19141 atgaaatact gtagctgtac cttccaccaa ggccagtcat gatacacatc ataaccccag
    19201 aaatcacacc aagcctcgat ttgttgatca taaaggatgg aactaaattg tcttaaagat
    19261 tcgacacatt cttgttccca attgccttgt cctacacaaa aaatcaattt ggattgttta
    19321 tacaattcca tatagggatg gtctaaagac atatttttca tataggcaca tggattatta
    19381 tgatatgtat tttcatcatg ataatcgcca tagaataatt ctgtatcata aataccagat
    19441 agggctaata aggtatcgaa ttgtgtagga aaccggaaga ttaaatttgc ggcatgcgtt
    19501 gcccctagac ttgctccagt caccatccat ttatcatagt tatccatttt ctcttgtaca
    19561 ctaggaatca attctgaaag aatatattca aaccacaact catgtttcaa catacgcacc
    19621 ttagtattat aaggataaga ccatgtttct aaatccaaag tatcacatgt tacgatcgtc
    19681 aattgattgg cttcgatcca tttagaaact tcatgaatca tgcctcgatc ttcccattca
    19741 aagaaacgtc cattttgact tggaatagcc aaacacaact taccgccttg accatagatt
    19801 ttatattcca tttcacgatc caaacaattt gaatactctt ttatatattg tacttgcatg
    19861 gtgttacccc tttcataaaa tttttgtttt attatagcac attttattga gttttaaggt
    19921 ttcttgcttt ataattgtac tgtttttgac aggaaagtga gtgacagtat gtccaataaa
    19981 gaaaaaattc ttaacattgc cgttatcgcc cacgttgatg ccggtaaaag tacgttagtc
    20041 gatgcgtttt taagacaaag tgatgtattc cgtgacaacg aagaagttgt taactgtatc
    20101 atggactcaa acgatctaga acgtgaacgt ggaattacga tttattctaa aaactgttcc
    20161 gttattcata acggtgtaaa aattaatatt gtagatacgc caggccacgc cgacttctca
    20221 agtgaagttg aacgtattat ccgtaccgta gatacagtta tcttattagt cgatgctagt
    20281 gaaggtccaa tgcctcaaac tcgtttcgta ttatcaaaag ctttggaaat tggtttaaaa
    20341 ccaattctat tgatcaacaa gattgataag aaagaccaaa gagctgaaga agttgtcgat
    20401 gaagtttatg aattattctt agacttaaat gcgaacgacg aacaattaga cttccctatc
    20461 ctttatggtg tagctagaga aggacgtgtt caatacgacc ttaaaactcc aagcgacaac
    20521 atcgacccat tattcgatac aatcttaaaa cattgcgatg tatatccaga tatggatgaa
    20581 gaacctcttc aaatgcaagt atctgccttg gcatacgatg aatacattgg tcgtttagga
    20641 attggacgtt tatattcagg tgttttaaaa caaggacaac aatatattcg ttgtaaccaa
    20701 tctggattga atgccaaaga aagcttatct aaactattcg tatacaaagg attatctaga
    20761 acaagcgttc aagaagcaca ttcaggagat atcgttgtta ttgcgggtat gccagatatc
    20821 agtattggtg atacaatctg tacagccgat cacattgaac caatggaaca cattgaaatt
    20881 gaagagccaa ctctatctat gaacttccat gttaactcaa gtccatttgc aggacagagt
    20941 ggtaaatatg tgacaagtcg taacttaaaa gaaagacttg aaaaagaact agaagtaaac
    21001 gttggattaa aggttgaacc aaccgattca gcggattgtt tcaaagttag tggtcgtggt
    21061 gaattacata tttccgtatt aattgaaaat atgcgtcgtg aaggttatga actagctgtt
    21121 tctaagccag aggttatcct acacaaagat gaaaatggac ataaagtaga accaatcgaa
    21181 gaagttgtat gtttagctcc agaagaatat tcaggaacaa tcatcaacaa attaaatctt
    21241 cgtaagggtg ttatgcaaga catgtctgaa gaaaatggat atgttaaaat cgtatataca
    21301 gcacctactc gtggactatt aggtttccgt tcagaattta tcaatgatac acatggtgaa
    21361 ggtacattag tacgtcgtat tagtggttat gaaccatata aaggagaaat tgcacaacgt
    21421 atggaaggtg ctatgatttc aactgaaaca ggtgaagcta tgacttacgc tttatggaac
    21481 cttcaagaac gtggtcaatt attcatttct ccacaaacac cagtttatga aggtatgatc
    21541 attggtcaaa gctctaaaaa cattgactta gatgttaacc cattaaagaa caagaaatta
    21601 acagccatcc gttcaagtgg tcgtgatgaa gccatgttat taactccacc aaaaatcttc
    21661 tcacttgaag aagcattaga atggatcaac gatgatgaat tagttgaagt tacaccagat
    21721 gctattcgta tccgtaaaaa aggtttaaca gctttagatc gtcgtaactt atatcgtcaa
    21781 aagatgaatg ctcaataagg cttggataat tccaagtctt ttttttaaag cagcatttta
    21841 gctgcataat caatcagtct ttaaacgaca ttctacagat ttagtgattt cttggttaaa
    21901 aatatcacgc attttaattt caaccatata ttgcgttgag ctcattaatt ttggaatacc
    21961 aatttcaaga gtatactttg ttgtatcaat atcaattaaa tcatttaaat ccgtaaactc
    22021 attaatagta tatgttttat taattttaga cgctacaatc atcattttaa aaaacgaatt
    22081 ctcttttatt agattattca ttttataatc ataactagga aaatagaaac tatgagcaat
    22141 tctccatata tcaataattt taggatcttc taattcttta tgtcctaaaa cattaccaag
    22201 cctggcttca ctcactacac gcagttctga tgttttcact aatgagtcat gttttaaaaa
    22261 aggaaaatca acttttttaa ttaaaacaaa tttctttgtt ccattcggat tttcaacttc
    22321 atgataagcg tttaaatgct gcctattatg actttttgga gttgttattg gcaaaacata
    22381 ataagtatga ttctgtttca taaaaataat accaatatgt tcaaaggaca attcaggttt
    22441 aggattatgt ccgaattcaa tccaaacgaa atcaccaact ttatatgatg aatcataact
    22501 tatgccacga tgataacttg cggtaataaa ttctaaagat ttcatggatt tattaacaac
    22561 ttgattgttt gatccatttg ataaatgata ccaataatct gttaaatttt tgatagcttt
    22621 tatacctgtt ttaaacatat tttcctccta aaaaaaaagg taggtgcaca acacctacca
    22681 cagtacgcgg gctcatcacc ctaagttcaa gaatcagtac gcgggctcat caccctaata
    22741 ctcatctcca caattcaatt atacaccaaa tataattaaa gacaatttaa atttaatatt
    22801 tcttacctta aacttttcgc aaatttatat cttaaactta cgtcgtatac gtcgttagta
    22861 caaggtgaac agtttttcaa agacattatt tcaacttctg cccgacatgt ttatcaaaat
    22921 tatgtctgat aaacacatta ctagatcata caatttcttg ttaaagacaa tcatttaaag
    22981 tataaggatt atctgtcttt ttcgttttaa cctctttaga ttctactgtg aaaacttaaa
    23041 catccaaaaa aaggtaggtg cacaacacct accaagttac gcgggctcat caccctaata
    23101 cttatctatt accagcttcc gccgcctccg ccaccggcgc ctccaccaga gaagccacca
    23161 aagctagagc ccatgctatc ggctgtgctt ttcatttgtt ctgtttgata atccattaaa
    23221 ttctcatgga ttggttcata catacttctt gtcagcattc tgtaatagaa atatgaagag
    23281 tatggatctg aataataaga atcataccaa tctggttgtg ccaaaggaat cgtgctaaat
    23341 ttctttgtcc acatttcaga taatccaaag accatcgcat atggaatcac atcataatag
    23401 taagatggat tttctaaaga tagtctttcc aattgtggta cttctgcctt ttcaacaaag
    23461 tctttaaaac ctaatagctc aggagctaca cttttgaagt agttacttac cacattaaat
    23521 ttagaaatca aaagaattgg tagtgaaaca accacaatgc ctatcatcat aaattcaaat
    23581 ggaacaatac ttagaatcga atcacattgg cgataaacaa aatatgaaag aattaaaata
    23641 aacgcaatac tcaaaatttt ctttaataca ccaagtttat tcttgttaaa tacatttgtt
    23701 gcagctgcag aataatttaa gaccatcata agcacaaagc taaatgttgc taaaatggtt
    23761 tcaatcaaat tatcaaggat actatatctt gtattaaata agatacatac aatcatacaa
    23821 accaaggcaa gaatggttgt cacaaaacca tgatcaatag tagaaagttt tttatcttca
    23881 ctaaatacat cattcaaagc ttctttggca tcatccatag ccttaccaaa cttacttgat
    23941 agttttgata atttcacact gtttttagtt ttaaacaatc cattataaat cttcttttga
    24001 tgttttaaaa tcggcggttc atgattctct tgcttagtaa gaaccaaatc atcctctttt
    24061 tcagaaatgg ttaaataccc cttacttgcc caatatggaa tcagggccat taaatcttct
    24121 tgatccactg actcatctac aatcgttcca accatagctg gatccatatc ttttggtgga
    24181 tagaaattta caatcggtgt aatatgcgtt ttctttaaaa gaagagcacg aatcaaactg
    24241 tataaaccaa atagaatggc caaacccaaa aagataaatg gaatttttga agtcgtttgt
    24301 ttggcaccca caaaatagcc ttgtcttaat ttaccaaaaa gagtaatagc ttgttttgca
    24361 ccaatatcat tggcagaacc actgatagaa gtcgaaataa taattggagt cacatttaaa
    24421 tcattcgatt gatttcctag acttccacta aaaacttcaa tattagacat ctcatcttct
    24481 gtcaaagcct tttcaaattg aatatcaaaa ctaaaatgat tgattgttgt atcccaattg
    24541 tttccaagta ccgaataata tataaaatca aaatccgaat gataatcttc tggaatgaca
    24601 atcgtatagg tcaatgtata ggtatgtggg ccaatgatcg tatagtctgc acttccaatt
    24661 tgaatcacac gatttccaga atcactatct acttcatact catcatt
//
LOCUS       DBMDWL010000009        24025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_783_length_24025_cov_3.113693, whole genome shotgun sequence.
ACCESSION   DBMDWL010000009 DBMDWL010000000
VERSION     DBMDWL010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 24025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24025
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_24025_cov_3.113693"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     misc_binding    171..381
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            488..1738
                     /locus_tag="ACZPDN_01200"
     CDS             488..1738
                     /locus_tag="ACZPDN_01200"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017144038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1297272.1"
                     /translation="MKKQYNKVVLAYSGGLDTSVLIPWLKENYGCEVIAVSGDVGQKS
                     ELDGLEEKALATGASKLYIADLKKEFVEDYIIPSMKAGASYENYLLGTSFARPIIAKK
                     LVEIAKQEGADAICHGCTGKGNDQVRFELAIQAFAPGMDIIAPWRFWELNSREKEIEY
                     AKAHNIPLKITAETNYSKDKNLWHLSHEGLDLENPSNAAPLEKEGFLELGVSPLQAKD
                     EPDYVTIHFEEGTPTSIDGKEMSCLDIIETLNDLGGQNGIGILDIVENRLVGMKSRGV
                     YETPGGTILYAAHEKLEEITLDKDTAHYKKQVALKFGELVYDGKWYTPLRKALSSFVD
                     ETQKTVTGDVKLQLYKGNIIPQSVTSPYSLYSSGMATFDEDDCYDQADSAGFIHLFGL
                     PLKVRALNDQELFEKSGKHFPDVE"
     gene            1746..3134
                     /gene="argH"
                     /locus_tag="ACZPDN_01205"
     CDS             1746..3134
                     /gene="argH"
                     /locus_tag="ACZPDN_01205"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1297273.1"
                     /translation="MAKMWAGRFKKDANVKLDSFNTSLPFDCRLYKQDIQGSLVHSKM
                     LEKQGILSKKDVDLIQEGLYSILDQIESNKIEFSHAEDIHMFIEEKLTNEIGDAGKKL
                     HTARSRNDQVALDMRLYVKEEIIEIQKLLFQLLECLCTSAKSNLKTYMPGYTHLQRAQ
                     PITFAHWCMAYANMFKRDIVRLQNTYSLMNECPLGCAALATTTYPIDREYTASRLDFL
                     KPTDNSLDGVSDRDYCVELCSDLSLIMVHLSRMSEEIIQWCSLEFHFVELDDAFSTGS
                     SIMPQKKNPDICELIRGKSGRTIGDLNTLLVMLKGLPLAYNKDMQEDKEAVFDCIDTV
                     KECLDVFIPMFQTMKICKDNMMHACLHGFINATDLADYLTKKGLPFRDAYHIVGQIVG
                     YGIENKKSLNELSLKEYKKFHASFEEDVYQYIDIETCVNNRNVYGGPSPESVSIQIQN
                     IEKFISLEKTYD"
     gene            3127..4023
                     /gene="argC"
                     /locus_tag="ACZPDN_01210"
     CDS             3127..4023
                     /gene="argC"
                     /locus_tag="ACZPDN_01210"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1297274.1"
                     /translation="MIKVYIDGQAGTTGLEIVKRLQDRSDIKLLMIPEDKRKDVETRK
                     EYLNSADLVFLCLPDKASVEAVSLITNSNVKVIDASTAHRTVWTYGFSELGLKDKIAS
                     SQYVANPGCHASGCISILYPLIQKGLVNQTEQFSFFSLTGYSGGGKKMIYQYENEKTI
                     DLEAPRIYGLNQYHKHLPEIKKVCGLEKDVFLNPIVCDYYRGMAVSLNVEMDYEKVYL
                     VLKDWYSDSTLIQVEKVDSGMISANTLAGLDCMKLIVSGNSKRCTITSLFDNLGKGAC
                     GAAIQNMNIMLGFKEEEGLNLE"
     gene            4020..5210
                     /gene="argJ"
                     /locus_tag="ACZPDN_01215"
     CDS             4020..5210
                     /gene="argJ"
                     /locus_tag="ACZPDN_01215"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR1297275.1"
                     /translation="MKVIENGVCAATGFKAGSIHCGIRKNQTKDDLALIVSDTMCQTA
                     AVYTKNKVKAAPILVTKKHLINGMSQAIIVNSGNANACNPKEIENAQLEAQCAADYLN
                     VDVENVLVSSTGVIGQVLPIEKIQDKVSDINLSAENNSNAAKAIMTTDTKEKTVAVEF
                     KMGSVTCHMGGICKGSGMIHPNMGTMLAFITTDCKVEQNVLQSCLLDNTNKTFNRVSV
                     DGDTSTNDMCIVMANGMAQNEVFDAEVFDQALHFVMEELAKKIAADGEGAGKLMTVTV
                     NGVDTLENAETLAMSVCSSSLVKAAMFGADANWGRVICALGYSGVEFDPELVDISFKS
                     NDEILCCESGKGLDFDEEKAKSILLQEEIEIICDMHCGNESCTCWGCDLTYDYVKING
                     DYRT"
     gene            5207..6034
                     /gene="argB"
                     /locus_tag="ACZPDN_01220"
     CDS             5207..6034
                     /gene="argB"
                     /locus_tag="ACZPDN_01220"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR1297276.1"
                     /translation="MIDANVLVEALPYVKEYSGQIVVVKYGGNAMIDEELKAAVIKDV
                     TLLNLLGVHVVLVHGGGPEINEMLSKTNKESKFLNGLRVTDDETMDIVQMVLCGKVNK
                     NLVNLLDKGIGLSGVDGSLFEASCLDSKLVNVGKIEKVNPQIILDTFENGYIPVVSSV
                     AKAIDKLGTYNINADLAASELAISLHAKKLILLTDVKGLMKDPKDESTLMERVKVSQI
                     PLLKKEKVITGGMIPKIECCIKAVREGVESVNIQDGRVMHSLLIELLSDEGVGTLIE"
     gene            6045..7205
                     /locus_tag="ACZPDN_01225"
     CDS             6045..7205
                     /locus_tag="ACZPDN_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017144639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR1297277.1"
                     /translation="MEESIYLMYTYNRFNIEMESGEGCYLKDTNGKEYLDLTSGIGVN
                     CLGHNHPVLVEAIQKQVATLMECSNLYYSKPMVDAAKKLVEYTGLSKVFFSNSGAEAN
                     EGMIKLARKYSYDKYGVGRDVIITLKQSFHGRTITTLKATGQDKFHQYYFPFTEGFDY
                     AAANDIEDLKRVANEKTCAVMVELIQGESGVVPLDKDYVKEVEEFCHSKDILFLVDEV
                     QTGIGHTGTLFCYEQYNVLPDVITTAKALGGGVPIGAVITGAKCKDTFGPGDHGSTFG
                     GNPLSCKAADTVLSIVTEHGFLDHVKVVGAALKTGIENIHSDKIKEVRGIGLMLGIVV
                     DPDKRADYVQAMMDKGLLTLTAGKDAIRLLPPLTLSLKEVDEALSIMKEVFE"
     gene            7202..8116
                     /gene="argF"
                     /locus_tag="ACZPDN_01230"
     CDS             7202..8116
                     /gene="argF"
                     /locus_tag="ACZPDN_01230"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1297278.1"
                     /translation="MKHLLTMEQLSEKEILSILELADQLKYERKHHIEHPLLKGKSIG
                     LIFQKSSTRTRVSFETGAYQLGAQPLFLSSRDLQIGRGEPVQDTARVLSRYLDCIMIR
                     TYEQKEVEDLANFGFIPVINGLTDYCHPCQVLADLMTIREKYGLLKGLKMCYIGDGDN
                     MANSLIVGGLKVGMKVSVATPKGYEPDKHVLEFAKDFKDFELTNDCIKAIQDADVVIT
                     DTWASMGMEAEKEKRAKDFAGFQVNAELMKYAKENAMVQHCLPAYRGQEITEEVFEAH
                     ADEIFEEAENRLHAQKAVMVQLMKEKEE"
     gene            8249..8434
                     /locus_tag="ACZPDN_01235"
     CDS             8249..8434
                     /locus_tag="ACZPDN_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297279.1"
                     /translation="MRTIKYHLVLTDNEYSLIINSLNNLRIGLIAQSRYTDAVDDVFV
                     EFIGAKKKNFKIIYQEG"
     gene            8563..8787
                     /locus_tag="ACZPDN_01240"
     CDS             8563..8787
                     /locus_tag="ACZPDN_01240"
                     /inference="COORDINATES: protein motif:HMM:NF025561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIR1297280.1"
                     /translation="MRYYNLLTSERLYDYLDGVEYQAENLFEQTVKSLAEKEQVTEKL
                     KAENMMLWVQKTNNIRNRAIEIVNEQVIYK"
     gene            complement(9032..9790)
                     /locus_tag="ACZPDN_01245"
     CDS             complement(9032..9790)
                     /locus_tag="ACZPDN_01245"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HIR1297281.1"
                     /translation="MIFYFSATGNCKYVAQRIAASTGETTISIAECIKQQKFHCSSDN
                     GTVGIITPTYAWGLPSIVRDFLNVLVFDEKPSYLWFLSTYGTTSGQTGRFANEIMQKT
                     GLSFATYLSVKMPDTWTPTFDLSNPQKVRRINFDAEPEIDFAIEKIQSQSAGNYMRNK
                     VPTFAAKVFYGLEYDKMRKTDHFRVENTCIGCGLCARNCPVQAIEIHDKKPVWVKERC
                     VMCLSCLHHCPKFTIQYENRTKKHGQYTNPNVKL"
     gene            complement(9805..10566)
                     /locus_tag="ACZPDN_01250"
     CDS             complement(9805..10566)
                     /locus_tag="ACZPDN_01250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1297282.1"
                     /translation="MNIAIITGASSGLGAEFLRAVAEKHTELDEIWLIARRQEKMEQL
                     ITELPYQYCRILPLDLSSEEAYRELESILHSEKPNIRILINNAGYEKSGIFVEMEESD
                     IQKMLSVNVKGLTMVQRVCTPYMRSGSYAVLTCSVSSFTPVPHQAIYSATKKYVYFFG
                     KALREELLEQGVNVLLLCPGNMDTEMNPKGQGRQSKQINRLPFLDLKKLTRKALALAE
                     SGKAVYTPGAFYKGYRFVSKVFPSAFMLHFVRKFY"
     gene            10723..11289
                     /locus_tag="ACZPDN_01255"
     CDS             10723..11289
                     /locus_tag="ACZPDN_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1297283.1"
                     /translation="MKYNLTEPLTLGAKRTLEAFSRELFSLLSKKAFENITVGQLCEN
                     AQYPRATFYNYFDDKYDLLNYCWMSLAEKIGLSEYHYAPESQMLFLYFDRIYSFTKEH
                     IEVISKILFYNSEAGYMFSSLRNFMNSQMRIIFKDCPDALKREMPTELLADHYSNTLF
                     LVWQWTITKERSCDSQQAQNYLRKLLFL"
     gene            11515..11628
                     /locus_tag="ACZPDN_01260"
     CDS             11515..11628
                     /locus_tag="ACZPDN_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR1297284.1"
                     /translation="MTNGFAKKTQKAPKKEIKLAKERRKDFIERMMRNENI"
     gene            11615..11896
                     /locus_tag="ACZPDN_01265"
     CDS             11615..11896
                     /locus_tag="ACZPDN_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297285.1"
                     /translation="MKTFDKMLAKQMKDEEFKKEYEAIQPEMDVIRAIVDARTSQNLT
                     QKELSERTGINQADISKLENGTRNPSINLLKRLAKGMGMTLKIEFVPKH"
     gene            complement(12135..13232)
                     /locus_tag="ACZPDN_01270"
     CDS             complement(12135..13232)
                     /locus_tag="ACZPDN_01270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1297286.1"
                     /translation="MLETIKKYRRELHQIPELELDLPETTNYILSVLEELDCIVSVPL
                     KSTILAYFDNQKDHTLAFRSDMDALPVTEQTNLEFQSKNPGKMHACGHDGHMAMLLGF
                     AKELNTYYKTLDKNILLIFQPGEESPGGAHLICEAGILETYKVQYIFGTHLWPILEKG
                     VVATRKNEFMARASEINIDIYGKESHAAKYKEGIDALEIGAEILLEIYAMEKEIPNEY
                     YRLLRFGKMESGTVRNVVSAHTRLEGTLRAFQDYIFDSMKEEIFRICADKEKKYNCKI
                     DISINTGYPAVMNDEKLVDQLIHKIPEIRQLRNPEMIAEDFSYYQKEVPGVFFFLGTQ
                     TPYALHNAKFDFDEEVLLSGIELYKKITKII"
     regulatory      13325..13489
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            13556..14797
                     /gene="lysA"
                     /locus_tag="ACZPDN_01275"
     CDS             13556..14797
                     /gene="lysA"
                     /locus_tag="ACZPDN_01275"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR1297287.1"
                     /translation="MMKVKELVNTYGSPLYVYDQGMIEDKLKAFKTHLKSPDFECDVI
                     YASKAFNCKAMIQLVSKMDCSLDVVSGGELYTAYVAGFDMRRVYFHGNNKSMDELKLA
                     LKYRVGTIIVDNLMEAKVLSELARDIDYTVSILLRVNPGVEAHTHEYIVTANIDSKFG
                     ISIEKKSDILELINSINSCPNLSFDGFHAHIGSQIFDKEAFVQEVNKVCEFVSNLGVE
                     VSTFNFGGGFGAVYTSEDTPIPVDVVCDTLIKAVKCNIEKYKLDIHKICIEPGRSIVA
                     EAGSTVYTVGFMKETPHKNYIFVDGGMSDNIRPALYQAKYSCVIDGKENEMPSKHYKV
                     AGKCCESGDVIIEDAFLPEVKPNDLLIVKTTGAYGYSMASHYNKALGLPVIFCKDGKS
                     QCVIRGENYEDLIRLENDLCA"
     gene            14788..15582
                     /gene="dapF"
                     /locus_tag="ACZPDN_01280"
     CDS             14788..15582
                     /gene="dapF"
                     /locus_tag="ACZPDN_01280"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1297288.1"
                     /translation="MRIKFSKYHGLGNDFILTRYIEDIDIPTLVQSCCERHTGIGADG
                     MIFVKKDPLEMVYYNQDGSRAPMCGNGIRCFAAYCFDEGINIEETVPVATLAGQKIVH
                     RISTSPFEVCVDMEQPLYDNRLMDVSEDENVWKREVCGVDTYSLFMATTHTVVFVDDA
                     FDEKNIELGRKICHNPLFKKQTNVNFVQVIDDTHIRVMTYERGCGITLACGTGACASV
                     VVANKLGFVQSECEVQLKLGSLKIEVKDHVYMTGPAKKICEGEFVC"
     gene            15576..16451
                     /gene="dapA"
                     /locus_tag="ACZPDN_01285"
     CDS             15576..16451
                     /gene="dapA"
                     /locus_tag="ACZPDN_01285"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1297289.1"
                     /translation="MLKGSIVALVTPFHEDGTVNFEKLGELLDWQIENSTDGILILGT
                     SGESSTMSHEEDDAVALYTIERVNHRVPVIVGAGSNCTQTAMEKSLKYQNMGADALLL
                     ISPYYNKANTQGMIRHFTTVADAVHIPIILYNVPGRTGCHIDEKAVKVLSQHPNICGI
                     KEASGNISYATNIARYLSDDFVMYSGNDDMITSMMALGASGVISVLANCLPQETHEIV
                     QDYLDGNVKASLEKQLKYLDFIHALFIEVNPIPVKQALNYMGKDVGGYRLPLCEMDEK
                     NAKILKATMEDLGLC"
     gene            16445..17143
                     /gene="dapB"
                     /locus_tag="ACZPDN_01290"
     CDS             16445..17143
                     /gene="dapB"
                     /locus_tag="ACZPDN_01290"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1297290.1"
                     /translation="MLKIAIVGTGKMGSLLKETAQNQGMEVVHCFEKGIDDTSIDVDV
                     VIDFSHPNNLASVLNFVKIKKCALVYGTTGLSENQINSLKQVSDEIPLFYSANFSYGI
                     AVFEQILKQFAPLLKNDFDMEVQEIHHNQKQDAPSGTAKMLVNALDPEDEYTKVYGRS
                     GFVGKRQHEIGIHSLRGGTVAGEHTVYFFGDNETLEIKHSANSRQIFVNGALKAAQFV
                     VNQKPGLYSMEDMM"
     gene            17153..17830
                     /gene="dapD"
                     /locus_tag="ACZPDN_01295"
     CDS             17153..17830
                     /gene="dapD"
                     /locus_tag="ACZPDN_01295"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HIR1297291.1"
                     /translation="MEAEQIIRYIQMAEKKTPVKVYFKSEREISFPNCKVFGNVVFGD
                     WKDIEPVLLMNPEVDYEVENNCRNSAVPMLNLKNIHARIEPGAFIREQVEIGDNAVIM
                     MGAIINIGAVIGEGTMIDMGAVLGGRATVGKHCHIGAGAVLAGVIEPASATPVIIEDD
                     VLVGANAVVIEGVHVGKNAVVAAGAVVIDDVPENAVVAGCPARVIKMKDEKATQKTKL
                     EDALRNL"
     gene            18182..19435
                     /locus_tag="ACZPDN_01300"
     CDS             18182..19435
                     /locus_tag="ACZPDN_01300"
                     /inference="COORDINATES: protein motif:HMM:NF025019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297292.1"
                     /translation="MEIKRDAYLQQLIERRDNGMIKVITGIRRCGKSFLLFNIFKRYL
                     LESGVGVDHIIEIALDGIENEELRNPKVCFEYIKNAMKDDGKYYLLLDEVQFMPRFEE
                     VLNSLLRMSNIDVYVTGSNSKFLSKDIVTEFRGRGDEIRIYPLSFAEFYSVYDGDYDD
                     AWDDYMVYGGLPQVVGFQSERQKTDYLKNIFTNVYLKDVVERNKIQGVDEIGILVDVL
                     ASTIGAQTNPSKIAKTFASERQMTYANKTISYHIDCLADAFLISKASRYDIKGRKYIG
                     ANLKYYFTDLGLRNARLNFRQQEPTHIMENIVYNELLIRGYNVDVGVVDVFDKDKEGK
                     RIRKQLEVDFVVNQGNQRYYIQVAYDMTSEEKQVQEFKSLRNIPDSFKKIVIVNGSKK
                     PWRNDEGFVIMGMKYFLLNAGSLEF"
     gene            19836..20372
                     /locus_tag="ACZPDN_01305"
     CDS             19836..20372
                     /locus_tag="ACZPDN_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HIR1297293.1"
                     /translation="MEIKDAKVFHDYLNGVIEGEDVKKSTKLYSNAKTFYSSVDSSIS
                     DDQIMYEVYSYEHDGLCYGLTVMQPVCVNGECNVTRGHFHENLDCDEIYCGMGGEGLL
                     LLMDEDYHCHAEKVKCGSVHYIDGHLAHRLVNTGNTELKVQCMWLSHAGHDYKRVEEH
                     PFTVRVFKKNGELKVEEE"
     gene            20369..22093
                     /locus_tag="ACZPDN_01310"
     CDS             20369..22093
                     /locus_tag="ACZPDN_01310"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HIR1297294.1"
                     /translation="MSYLNFKSEYDRFPKTKIKNHKAFCGYEEIYRELKSKMNNNSVV
                     VFDYYPGVDEKEVYELIKRFNFSLEINMHDIFKDGKTMTEQMKYNLTDDRVFGKMYYG
                     NLVDFIDEEKLDEARNKIKEHKESVIVYGVGAGLVSHGDVYVYFDMARWEIQLRYRKG
                     MANYNATNFDEDILKKYKRGYFIEWRIADKHKAERFDCFDYVVDTNVANDPKMISRYT
                     FTESLKQLAQKPFRTVPYFDPGVWGGQWMKEVCNLDVNQPNYAWSFDGVPEENSILFD
                     YDGVTVELPAMDLVLYQPKELLGEQVYSRFGAEFPIRFDFLDTMEGQNLSLQVHPLTE
                     YIKKNFGMSYTQDESYYILDAKEDAHVYLGLKEGIQPNEMISDLESAQRGEIVFDADK
                     YINKFPAKKHDHFLIPAGTCHCSGKNAMVLEISATPYIFTFKLWDWQRLGLDGLPRPI
                     NIEHGKHVIQWDRTTKWVKENLVNATYEVKEDGNDCKIEHTGLHELEFIETRRYTIKN
                     SSYQQTHGTVNMLNLVDGKSALIEGDFEPFIVHYAETFIIPASIKEYTIKPYGCDEVK
                     VLKAYVRF"
     gene            complement(22155..22838)
                     /locus_tag="ACZPDN_01315"
     CDS             complement(22155..22838)
                     /locus_tag="ACZPDN_01315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1297295.1"
                     /translation="MRLDKYLSHMGFGTRNDVKKLIKNGWVTINDETIKKADYNVKDD
                     DRVCVDDEPVSYVEFEYYILNKPQGYVSATEDMLYPTVMELIQSQRHDLYPVGRLDVD
                     TEGLLLISNDGKLTHEVLSPKKHVDKKYYVEFDGTLPENAVDFFNQPMEFEEFTSAPS
                     KLEILDTNKAYLTIHEGKFHQVKRMFEKANCTVTYLQRIEFGPLKLNDLPLGEFRALT
                     QEELDALKG"
     gene            complement(22835..23899)
                     /locus_tag="ACZPDN_01320"
     CDS             complement(22835..23899)
                     /locus_tag="ACZPDN_01320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR1297296.1"
                     /translation="MLPAQVLPVDENDIVLDACAAPGGKSTKLATKLNHTGLLISNDI
                     SSSRCQGLLKNIELFGCDNAWVTSEDLTNMEQHYPETFDKILVDAPCSGEGMFRKEPD
                     LIKSWIEKDDTFYPPIQKNILNAAISMLKPGGSIVYSTCTFSIHENEEVIQDVLDKHP
                     DLHLVPINKIDGMMPGINMEECVRLYPHKIKGEGHFVALLVKDGETKHKKVRLEPSDK
                     LDDCVTDFLKLVHLNKGTIKVKKDKVIWLNTDFQAYKGYRVLRSGLLLGQLKGKHFEP
                     SQSLALALCPKQFKNILNFSIEDERVIKYLKGETLDVKDLDSKTSGWTLVCVNNFPLG
                     FAKLSKGSLKNKYAKGWRYQ"
BASE COUNT         7922 a         3414 c         5119 g         7570 t
ORIGIN      
        1 aaattaacaa caccgccttc aaccacggtt attaaaggtt gaaagaaagg agaagtatat
       61 tgtagtcgac cagttagatg ggtcaattac aatataccag ggatgtaaat aattgtaaac
      121 taatgtaaat ttacgcttga aaagtttcag aaaataaata taatagtctc ataaatactt
      181 tgaaggaaag aagtacaaga agatttcttc tttagagaga taacattttg gtgcaagtta
      241 tcgttgaata ttcttggaac gtgttctgga gtggtctagt cgaaggtttg aggctagaac
      301 gtgaattcgc gttaagaatg aggtgtcatc tgcatagggc agatggaatt ttggtggtac
      361 cgcgcttata acgtccaatt attttgggcg ttttttttta tggtaaggag atgatgaata
      421 tggaatgcga tcgacgatgg atttatagaa caattttttc atatacatta acaaggaggt
      481 acttatcatg aaaaaacaat acaataaagt cgttttagca tattctggag gattagatac
      541 atcggtatta attccttggt taaaagaaaa ttatggatgt gaagtcattg ctgtaagtgg
      601 agatgttggt caaaaaagtg aattagatgg ccttgaagaa aaagctttgg ctacaggagc
      661 tagtaaatta tatattgcgg atctaaagaa agaatttgtc gaagactata ttatcccaag
      721 catgaaggca ggggctagct atgaaaatta tttattagga acaagctttg cacgtccgat
      781 catcgcaaaa aaactcgttg aaattgccaa acaagaaggg gctgatgcga tctgtcatgg
      841 ctgtactgga aaaggaaatg atcaagttcg ttttgaacta gccattcaag cctttgcccc
      901 aggcatggat atcattgcac cttggagatt ctgggaatta aattctcgtg aaaaagaaat
      961 tgaatatgcc aaggcacaca atattccatt aaagattacg gctgaaacaa attattcaaa
     1021 agataaaaac ttatggcatt tatctcatga aggtttagat ttggaaaatc ctagcaatgc
     1081 agcaccatta gaaaaagaag gcttcttaga acttggtgta agtccattac aggcaaagga
     1141 tgaaccggat tatgtaacga ttcattttga agaaggaaca ccaacaagta ttgatggaaa
     1201 agaaatgtca tgcctagata ttattgaaac attaaatgat ttaggtggac agaatggtat
     1261 tggcattttg gacattgtag aaaaccgttt agtgggtatg aaatcaagag gtgtatatga
     1321 aacaccaggt ggaactattc tatatgccgc tcatgaaaaa ctagaagaaa tcactttaga
     1381 taaagataca gcacactata aaaaacaagt cgccttaaaa tttggtgaac ttgtatatga
     1441 tggaaaatgg tatacgccat taagaaaagc attgtctagt tttgtggatg aaacacaaaa
     1501 gacagtgaca ggcgatgtca aacttcaatt gtataaagga aatatcattc ctcaaagtgt
     1561 aacaagtcca tacagtttat attcaagtgg catggccaca tttgatgaag atgattgtta
     1621 tgaccaggca gacagtgctg gattcatcca cttatttgga ttgccattaa aggtacgtgc
     1681 cttaaatgat caagaattat ttgaaaagag cggaaaacac tttccggatg tagagtaggt
     1741 gttctatggc aaagatgtgg gccggcagat ttaaaaagga tgccaatgtg aaactcgact
     1801 ctttcaatac gtctttacct tttgattgtc gtctatataa acaagatatt cagggttctt
     1861 tagtccattc aaaaatgttg gagaaacaag gaattttatc taaaaaggac gttgatttga
     1921 ttcaagaagg attatattcc atcttggatc agattgaatc caacaaaatt gaattctctc
     1981 atgcggaaga cattcatatg tttattgaag aaaaattgac aaatgaaatt ggtgatgccg
     2041 gtaagaaatt acatacagcg cgttcaagaa atgatcaggt tgccttggat atgcgtttat
     2101 atgtgaagga agaaattatt gaaattcaaa aattattatt tcaattattg gaatgtctat
     2161 gtacaagtgc taaatcaaat ttaaaaacat atatgcctgg ctacacacat cttcaaaggg
     2221 cccaacccat cacttttgcg cattggtgta tggcttatgc gaatatgttt aaaagagata
     2281 ttgtgcgact tcaaaatacg tattccttaa tgaatgaatg tcccttaggc tgtgcagctt
     2341 tagctactac aacctatcca attgatagag agtatacggc aagtcgtcta gactttctta
     2401 aaccaactga taactcttta gatggagtca gtgatcgaga ttattgtgta gaactatgtt
     2461 cagatttatc actcattatg gtgcacctat ctagaatgag tgaagagatt attcagtggt
     2521 gttctttaga atttcatttt gtcgaacttg acgatgcgtt tagtacagga tcaagtatta
     2581 tgccacaaaa gaaaaatccc gatatttgcg agttgattcg tggaaaaagt ggtagaacta
     2641 ttggagattt aaataccttg ttggttatgt taaaaggtct tcctttggcc tacaacaaag
     2701 atatgcagga agataaggaa gccgtattcg attgtattga tactgtgaaa gaatgtttag
     2761 atgtctttat tcctatgttt caaacaatga aaatctgtaa agataatatg atgcatgcct
     2821 gtttacatgg ctttatcaat gcgacagacc ttgcggatta tttaacgaag aaaggtttgc
     2881 catttagaga tgcttatcat attgtgggac aaatcgtagg atatggtatt gaaaataaaa
     2941 aatcattgaa tgaattatct ttaaaagaat ataaaaagtt tcatgcttct tttgaagaag
     3001 atgtatacca atatattgat attgaaactt gtgtaaacaa tcgaaacgta tatggtggac
     3061 caagtccaga aagtgtatcg attcagattc aaaatataga aaaattcatt tctttggaga
     3121 aaacttatga ttaaggtata tattgatgga caagctggta caacaggact tgaaattgta
     3181 aaaaggctac aagatcgtag tgatataaaa ttgttgatga tacctgaaga taaaagaaaa
     3241 gatgtagaaa caagaaaaga atatttaaat tcggccgatt tggttttcct ttgtttacca
     3301 gacaaggcaa gtgttgaagc tgtaagcttg atcacaaatt caaatgtcaa agttattgat
     3361 gcttcaacag cacatcggac tgtgtggaca tatggattta gtgaattagg cctgaaagat
     3421 aaaattgcct caagtcaata tgttgccaat ccaggctgtc atgcatcagg ctgcatatct
     3481 attttgtatc ctttgattca aaaaggattg gtgaatcaaa cagaacagtt ttcttttttc
     3541 tctttaactg gatattctgg tggaggaaaa aagatgatct atcagtatga aaatgaaaaa
     3601 acaatcgatt tagaagcgcc aagaatttat ggcttaaatc agtatcataa acacttacca
     3661 gaaattaaaa aggtgtgtgg attggaaaag gatgtgtttt taaatccgat tgtctgcgat
     3721 tactatcgtg gtatggcagt tagtttaaat gtggaaatgg attatgagaa ggtatatctt
     3781 gttttaaaag attggtattc agattcaact ttgattcaag ttgaaaaggt tgattcagga
     3841 atgattagtg ccaatacact agcaggactt gattgtatga aattgattgt atctggtaat
     3901 agtaaaagat gtacgatcac aagtctattt gataatttag ggaaaggcgc ttgtggagcc
     3961 gccattcaga atatgaatat tatgttaggt tttaaggaag aggaaggatt gaatttagaa
     4021 tgaaagttat agaaaacggt gtatgtgccg ctactggctt taaagcgggt tctatacatt
     4081 gtggaattcg taagaatcag acaaaagatg atttagcttt aattgtatcc gatacaatgt
     4141 gtcaaacggc agccgtatat acaaaaaata aagtaaaggc agctcctata cttgtcacaa
     4201 aaaaacattt aataaatggt atgagccaag ccattattgt caattctgga aatgcgaacg
     4261 catgtaatcc aaaagaaatt gaaaatgcac aattagaagc acaatgtgca gctgattatt
     4321 taaatgtgga tgtagagaat gtgttagtct catcaacagg agtgattgga caggttttgc
     4381 ctattgaaaa gattcaagac aaagtaagtg acattaattt atcagcagag aataattcaa
     4441 atgcagctaa ggctattatg acaacagata cgaaagaaaa gacagtggct gttgagttta
     4501 aaatgggaag tgtcacatgt catatgggag gtatttgtaa aggaagtgga atgattcatc
     4561 cgaatatggg tacaatgcta gcttttatca ctacagattg taaggtggaa cagaatgttc
     4621 ttcagtcttg tttactcgat aacacaaata aaacatttaa ccgtgtcagt gtggatggag
     4681 atacttctac taatgacatg tgtattgtga tggctaacgg catggcacaa aatgaagtgt
     4741 ttgatgcaga agtatttgat caagctttac attttgtgat ggaagaactc gcaaaaaaga
     4801 ttgcggccga tggcgagggt gctggtaaat taatgacagt aactgtgaat ggtgtagata
     4861 ctttagaaaa tgccgaaact ctggcaatgt ccgtatgttc atcttctttg gttaaggcag
     4921 ccatgtttgg tgcggatgcc aactggggaa gagtcatttg tgcacttggc tattcaggtg
     4981 tggaatttga tccagaactt gtagatattt catttaaatc caatgatgaa attctttgtt
     5041 gcgaaagcgg gaaaggcttg gattttgatg aagaaaaggc gaaatccatt cttttacagg
     5101 aagaaattga aattatttgc gatatgcatt gtggaaatga gtcatgtact tgttggggat
     5161 gtgatctaac atatgactat gtaaaaatca atggagatta tagaacatga tcgatgcgaa
     5221 tgtacttgta gaggctttgc cttacgttaa agaatattca ggacaaatcg tagttgtgaa
     5281 atatggtgga aatgccatga ttgatgaaga attgaaggct gccgttataa aggatgtaac
     5341 cttattaaat ctattagggg tacacgttgt tttagtacat ggtggtggtc ctgaaattaa
     5401 tgaaatgttg tctaagacga acaaagaatc aaagttttta aatggtttac gtgttacaga
     5461 tgatgaaaca atggatattg ttcagatggt tctttgtgga aaagtaaaca aaaatctagt
     5521 caatctatta gataagggaa ttggattgag tggagtcgat ggtagtttgt ttgaggcgag
     5581 ttgtttagat tcaaaactgg tcaacgtagg aaagatagaa aaggtaaatc cccaaattat
     5641 tttagatacc tttgaaaatg gatatattcc cgttgtttca agtgtggcta aggcaataga
     5701 taaacttgga acctacaata ttaatgccga tcttgcagct agtgagctgg cgattagtct
     5761 acatgccaaa aaattgattt tattgacgga tgtgaagggc ttgatgaaag atcctaagga
     5821 tgaatcgact ttgatggaaa gagtcaaagt gtcgcagatt cctttattaa agaaggaaaa
     5881 agtaattacg ggtggcatga ttcctaaaat tgaatgttgt atcaaagctg tacgagaagg
     5941 cgttgaaagt gtcaacattc aagatggaag agtcatgcac tctttgttga tagaactatt
     6001 aagtgatgaa ggtgtaggaa ccttgattga ataggaggaa agttatggaa gaatcaatat
     6061 atttaatgta tacctataat cgtttcaaca ttgaaatgga aagtggtgaa ggatgctatt
     6121 taaaagacac aaatgggaaa gaatatctcg atttaacaag tggaattggt gtgaattgtt
     6181 taggtcataa tcatcctgtg cttgtagaag ccattcaaaa acaagtcgca accttaatgg
     6241 aatgttccaa cttgtactac agtaaaccta tggtggatgc tgctaaaaag ttagttgaat
     6301 atacaggact tagtaaggta ttcttttcaa attcaggagc agaagccaat gaaggaatga
     6361 tcaaacttgc tagaaaatat tcctatgata aatatggagt aggaagagat gtcatcatca
     6421 ctttaaaaca atctttccat ggtcgtacca tcactacttt aaaggcgact ggacaagata
     6481 aatttcatca atattatttt ccatttacag aaggttttga ttatgctgcc gccaatgata
     6541 ttgaagattt aaagagggta gcgaacgaaa aaacgtgtgc cgttatggtg gaattgattc
     6601 aaggggaaag tggagttgtt cctttggata aagactatgt caaagaagta gaagaattct
     6661 gtcattctaa agatatttta tttttggtgg atgaagtaca aacgggaatt ggtcatacag
     6721 gtaccttgtt ttgttatgaa caatataatg tgttgccaga tgttatcacg accgcaaaag
     6781 cattaggtgg aggtgttcca attggtgctg tgattacagg agccaagtgt aaagatacat
     6841 ttggtccggg agatcatggt agtacgtttg ggggcaatcc attaagttgt aaagcagccg
     6901 atacagtttt atctatcgtg acagaacacg gttttctaga tcatgtaaaa gttgtaggtg
     6961 cagctttaaa aacaggtatt gaaaatattc attcagacaa gatcaaagag gttcgtggaa
     7021 ttggtttgat gcttggaatc gttgtagatc cagataaaag agccgattat gtacaggcaa
     7081 tgatggataa aggattatta acacttacgg caggtaagga tgcgattcgt ttacttccac
     7141 cattgacatt aagcttaaaa gaggtggatg aagcattatc aattatgaag gaggtgtttg
     7201 aatgaaacac ttattaacga tggaacaatt gagtgaaaaa gaaattcttt ccattttaga
     7261 attggcggat caattaaaat atgaaagaaa acatcatatt gaacatcctt tattaaaagg
     7321 aaaaagtatt ggcttgatct ttcaaaagtc tagtacaaga acgcgtgttt cctttgaaac
     7381 aggggcctat caattaggag cccaaccttt atttttatca agtagagatc tacagatcgg
     7441 aagaggtgag cctgtacaag atacagctcg tgttttatcg cgttatttag attgtattat
     7501 gattcgtaca tacgaacaaa aagaagtgga agatttggca aactttggat tcattcctgt
     7561 tattaatgga ttaacagatt attgtcatcc atgccaagtg ttggcagatc ttatgaccat
     7621 tcgtgaaaaa tatggtttat taaaaggttt aaagatgtgc tatatcggag atggtgataa
     7681 tatggccaat tctttgatcg taggtggatt aaaggtaggc atgaaagtca gtgtagccac
     7741 tcctaaagga tatgagcctg acaagcatgt tttagagttt gcgaaagatt tcaaagactt
     7801 tgaactcaca aacgattgta ttaaggctat tcaagatgcg gatgttgtca ttacagatac
     7861 ttgggcaagt atgggtatgg aagctgaaaa agaaaagcgt gctaaagatt ttgcgggttt
     7921 ccaggttaat gctgaattaa tgaaatatgc caaagaaaat gcgatggtgc aacattgttt
     7981 accagcctat cgtggtcaag aaatcacgga agaagtcttt gaagcacatg cagatgagat
     8041 ttttgaagaa gcagagaatc gtttacatgc acaaaaggct gtcatggttc aattgatgaa
     8101 agaaaaagag gaatagtttg aaaaggatga atcactagtt gtaggctttg actgtagaag
     8161 atcagagcct tttttctatg ttttgaaaat ccgcacattc gtaatttaaa aatcaatcga
     8221 gtagaatata gtcaaggaga gattcacaat gagaactatc aaataccacc ttgtactgac
     8281 cgataacgaa tacagtttaa ttatcaattc actaaataac ctaagaatcg gcttaatcgc
     8341 gcagagcaga tataccgatg cagttgatga tgtgtttgtt gagtttatcg gtgcaaagaa
     8401 aaagaatttc aaaataattt atcaggaagg ctgaccatga atgtgcagta tgaacagcaa
     8461 ggcaattatc ttattccttg cataagaacc aaggagcaag aagaaataca ttttggcgtg
     8521 taggcaaacc ggcacaggcg atacctaaag cagagctaga aggtgcgata ttataatctt
     8581 ttgacgagcg aaagactcta tgattatctt gacggtgtcg agtatcaagc agaaaatttg
     8641 tttgaacaga cagtaaaatc acttgccgaa aaagagcaag ttacagagaa actcaaagca
     8701 gaaaatatga tgttgtgggt acaaaaaaca aataatatcc gcaaccgagc aatagaaatt
     8761 gttaatgaac aagtgattta taagtaagaa aaaggcacaa cttacgaaga agaaatccgt
     8821 agactgtgcc ttacttatat ttgtttgatt aaaatgtata atcttagcga gcagaccatt
     8881 tgtttattca aacagtaaaa cactcgttag ggacatccta agacctggat ataactacaa
     8941 agaattataa tgtttttata tattcgttat actcaatgat aggaataaca ccaccggaag
     9001 ggatatgaca aaagtctagc gaaaatttta atcataattt cacatttgga ttggtatatt
     9061 gtccgtgctt ttttgttcga ttttcatatt gaatggtgaa cttcgggcaa tggtgcaggc
     9121 aggacaagca catcacacac cgctctttaa cccagactgg ttttttatcg tgaatctcaa
     9181 tcgcctgaac gggacaattc cgtgcgcaca gcccacaacc gatgcaggtg ttttctaccc
     9241 tgaaatggtc ggtctttcgc attttgtcgt attctagccc atagaatact tttgccgcaa
     9301 aggtagggac tttgttcctc atatagttgc cggcagactg gctctgtatt ttttcgattg
     9361 caaagtcgat ttccggttcg gcgtcaaaat tgatgcggcg aaccttctgc ggattgctga
     9421 ggtcaaaggt cggtgtccac gtgtctggca ttttcacgct gagatacgtt gcaaaagaaa
     9481 gccctgtttt ctgcatgatt tcattggcaa agcgtcctgt ttgtcctgat gttgtaccat
     9541 atgtggaaag aaaccaaagg taagacggtt tctcatcaaa cacaagtaca tttaggaaat
     9601 cccgcacgat gctaggcagt ccccaagcgt aagtgggtgt aatgattccg accgtgccgt
     9661 tatctgaaga acaatgaaac ttctgctgtt tgatacattc ggcaatcgaa atcgtcgttt
     9721 ctccggtaga tgccgcaatg cgctgcgcaa catatttaca gtttccggtt gcagaaaaat
     9781 aaaaaatcat agtcacacac tccctcaata gaatttccga acaaaatgaa gcataaacgc
     9841 cgatgggaat actttgctga caaagcgata gcctttatag aaagcaccgg gggtataaac
     9901 agctttcccg gactccgcta gtgcaagagc ttttctcgtc aactttttca gatcaaggaa
     9961 cggcagacgg ttgatctgct tgctttgccg cccctgtccc ttcggattca tttccgtgtc
    10021 catatttccg ggacatagca gcagcacatt gacgccctgc tcaaggagtt cttcccgcaa
    10081 cgcttttccg aaaaagtaaa catacttttt ggtggcactg tagattgcct gatgcggaac
    10141 cggcgtgaaa gaggatacgg agcaggtcag cactgcataa cttccacttc gcatatatgg
    10201 agtgcatact cgctgcacca ttgttagtcc ctttacattg accgacagca tcttctgaat
    10261 atccgattct tccatttcga caaatatacc ggatttttca taaccggcgt tgttgatgag
    10321 aatccgaata ttaggctttt cactgtgtag aatggattca agctcccgat atgcctcttc
    10381 actgctgagg tcaagcggca aaattcgaca atactgatat ggtagttctg ttatcagttg
    10441 ttccattttt tcttgccgtc tggcaatcag ccatatttca tccagttcag tgtgcttttc
    10501 tgccaccgca cgcaaaaact ccgctcccaa tccggaagac gctcctgtaa taattgcaat
    10561 attcataata cgctctcctt ttgcagttgt cctgattttg acacttgtaa aaatcttgac
    10621 tatatgatac gatacaatca aatgcggaac aagtcacaga atcggtgatt cgtttagatt
    10681 tggacaaatt ttgaaaattg caaaagtgag gacattatcg ttatgaagta taatttgaca
    10741 gagccgttga ctctcggagc caagcgaaca ttggaagcat tcagtagaga gctcttctca
    10801 ctgctttcga aaaaagcgtt tgaaaatatc acggtcggtc aactgtgtga aaatgcacag
    10861 tatccaaggg caacctttta taattatttc gatgacaaat acgacttgtt gaattactgc
    10921 tggatgtcgc ttgcagaaaa aatcggactt tcggaatacc actatgcacc ggaaagtcaa
    10981 atgttgtttt tgtattttga cagaatctac agttttacaa aagagcatat tgaagttatt
    11041 tcaaaaatat tattttataa ttctgaagcc ggatatatgt tctcaagcct tcgtaacttt
    11101 atgaatagcc aaatgaggat tatttttaaa gattgcccag acgcattgaa gagagaaatg
    11161 cccacagagc tcttggcaga tcactacagt aacacacttt ttcttgtgtg gcagtggaca
    11221 ataacaaagg agcgtagctg tgattcacag caggcacaga actatcttcg aaagctactg
    11281 tttttataat atgcaattaa aaataaatca tcaacacatt aaccatcagg gagtactttc
    11341 tctggtggta tttttattta ctgaagtagg ttgtttttat gatattctac ccggagagcg
    11401 ataattttag gtaaatatat gtacaagttt attgtggaaa tttaggctaa atctctgttg
    11461 gatttgcaga actttctgta ttccaatatt gttcttacgt tatgtagttc tggaatgaca
    11521 aatggttttg caaaaaagac ccagaaagcc cctaaaaaag agatcaaatt agcaaaagag
    11581 agaagaaaag attttataga gaggatgatg agaaatgaaa acatttgata agatgcttgc
    11641 aaaacagatg aaggatgaag aatttaaaaa agaatatgaa gccattcaac cggaaatgga
    11701 tgtaatcaga gccattgtag atgctaggac ttcgcagaat cttacacaaa aagaattgtc
    11761 tgagcgtaca ggtattaatc aggcagatat tagcaaactt gagaatggaa ctagaaatcc
    11821 gtctattaat ttattaaaaa gattagctaa aggtatggga atgacactta aaattgagtt
    11881 tgtgccaaag cactaattat atttttaaat attatattgt tgtttggcag tcagttaatt
    11941 atggattaac tgctttgtaa atcttttctg ctatgataga ttatataaaa gttagttttt
    12001 ttgattatga agaaaatgct tttcagcaga tgcaggataa gattatttgt ccagcaagtc
    12061 atgaataaga cgaaaaaaag gaactgaact ccataagtaa tgaaaattac ttactcgtta
    12121 cagctccttc gtttttaaat gattttagtg attttcttgt ataattcaat accacttaat
    12181 aagacttctt catcaaaatc aaacttggca ttatgtaatg cgtatggagt ttgtgttcct
    12241 aaaaagaaga atacgccagg cacttctttt tggtagtacg aaaagtcttc ggcaatcatt
    12301 tctgggtttc ttaattgtct gatttctgga attttatgga ttaattgatc tactaatttt
    12361 tcatcgttca taacagctgg atagcctgta ttgattgaaa tatcgatttt acaattgtac
    12421 ttcttttctt tatctgcaca aattctaaat atttcttctt tcatagaatc aaagatataa
    12481 tcttgaaaag cacgtaatgt tccttcaagg cgagtatgag cgctgactac atttcttacc
    12541 gtaccgcttt ccatttttcc aaagcgaagt aagcgataat attcatttgg aatctctttt
    12601 tccatcgcat aaatttctaa aaggatttca gccccaattt ctaaggcgtc aatgccttct
    12661 ttatatttgg cggcatgact ttctttacca taaatatcaa tattgatttc gctagcccga
    12721 gccataaatt cattttttcg tgtggcaact acgccttttt caagaatagg ccaaaggtgt
    12781 gttccaaaga tatattgaac tttatatgtt tctaatatac cagcttcaca aatcaaatgc
    12841 gcacctcctg gactttcttc tccgggttgg aaaattaaaa gaatgttttt gtctaatgtt
    12901 ttataatatg tatttaattc tttcgcaaat ccaagaagca tagccatatg cccatcgtgt
    12961 ccgcaggcat gcattttacc aggattttta ctttgaaatt ctagatttgt ttgttccgtt
    13021 acaggaaggg catccatatc actacgaaag gcgagtgtgt gatctttttg attgtcaaag
    13081 taggcaagta ttgtactttt aagtggtaca gaaactatac aatctaactc ttctaataca
    13141 ctaagaatgt agtttgtggt ttcgggtaaa tcgagttcta attctggaat ctggtgtagt
    13201 tctcttctat attttttaat ggtttctaac atataattct ccttttcaaa ctattttaca
    13261 taaaaatgta ataatttaca attaaacttg aaacaacttg taagtggtgc tataattttt
    13321 ccatcttaaa gatagaggcg caaataagac aagtaaatac agagctggca agcgttggat
    13381 gtatttatga agcaagtttg ccgaacatgt tttttaggca taaaaaatgt gtgggccaat
    13441 ggagaatatc tgttggactg tctacgcaag taggagtgct atcaaatgat tgtgttttat
    13501 acatgtcagt ttgttagctg gcatgttttt ttgttttaaa gaaagggatt tatttatgat
    13561 gaaggtaaaa gaattagtaa atacatatgg aagtcctttg tatgtatatg atcaaggaat
    13621 gattgaagat aaattgaagg cgtttaagac acatttaaaa agtcctgatt ttgaatgtga
    13681 tgtgatctat gcatccaagg cctttaactg taaggctatg attcaattgg tttccaagat
    13741 ggattgttct ttagatgttg tgagtggtgg agaattgtat acggcatatg tagctggttt
    13801 tgatatgcgt cgtgtttatt ttcatggcaa caataaatcg atggatgaat taaaattggc
    13861 actgaaatat agagttggaa cgattattgt cgataatctt atggaggcta aggtgttgag
    13921 tgaactagct agagatattg attatacggt gtccatttta ttgagagtga atccgggagt
    13981 cgaagcacat acgcatgaat atattgtgac agcgaatatt gatagtaagt ttggaatttc
    14041 aattgagaaa aaatcagata ttttagaatt gatcaatagt attaattctt gcccaaactt
    14101 atcttttgat ggatttcatg cacatattgg ttcgcaaatc tttgataagg aagcctttgt
    14161 acaagaagtc aataaggtgt gtgagtttgt ttccaattta ggtgttgaag tatccacatt
    14221 caattttggc ggtggatttg gagctgttta tacaagtgaa gatacaccca ttcctgtaga
    14281 tgtggtgtgt gatactttga ttaaggccgt gaaatgtaat atagaaaaat ataaactaga
    14341 tatccataag atttgtattg aaccaggacg tagtattgta gcagaggccg gaagcacagt
    14401 ttatacagtt ggttttatga aggaaacgcc tcataaaaat tatatctttg tggatggagg
    14461 tatgagtgat aatattcgtc cagcattgta tcaggctaag tatagttgtg tgattgatgg
    14521 aaaagaaaat gaaatgccaa gtaaacacta caaagttgcg ggtaagtgtt gtgaatctgg
    14581 agatgtgatt attgaagatg cgttcttgcc tgaggtgaaa ccgaatgatt tattaattgt
    14641 aaagactaca ggagcgtatg gctattctat ggcaagtcac tacaataaag cacttggatt
    14701 acccgttatc ttttgtaagg atggaaaaag tcaatgtgtg attcgtggtg aaaattatga
    14761 agatttgatt cgattggaga atgatttatg cgcataaaat ttagtaagta tcatgggtta
    14821 ggaaatgatt ttattcttac aagatatatt gaagatattg atattccaac attggttcaa
    14881 tcttgttgtg aaagacatac gggtattgga gcggatggaa tgatttttgt gaagaaagat
    14941 ccacttgaaa tggtctacta caatcaggat ggttcaagag cccctatgtg tggcaatgga
    15001 atccgttgtt ttgcggcgta ttgttttgat gaaggaatca atatagaaga aacagttcct
    15061 gttgctacgc ttgcaggaca aaaaatcgtg catcgtatat ctacatctcc ttttgaagtt
    15121 tgcgtagata tggaacaacc gttgtatgac aatcgtttga tggatgtatc tgaagatgag
    15181 aatgtgtgga aacgagaagt ttgcggagtg gatacataca gtttatttat ggctacaacg
    15241 catacggttg tctttgtgga tgatgcgttt gatgaaaaga atattgaatt gggaagaaag
    15301 atttgtcaca atcccttatt taagaaacag accaatgtga attttgtgca agtcatcgat
    15361 gatacacata ttcgagttat gacttatgaa agaggttgtg gtattacgct tgcgtgtgga
    15421 acgggggctt gtgcaagtgt tgtggtcgca aataaattgg gctttgttca gagtgaatgt
    15481 gaagttcaat taaaattagg aagtttaaaa atagaagtaa aggaccacgt atatatgacg
    15541 ggtcctgcaa aaaagatatg tgaaggagaa tttgtatgtt aaaaggatca attgtagctt
    15601 tagtaacacc attccatgag gatggtacag tgaattttga aaagttaggc gaattgttgg
    15661 attggcaaat tgaaaattca acggatggta ttttgatttt aggaacatct ggagaaagta
    15721 gtacaatgag ccatgaagaa gatgatgcgg tagctcttta tacgattgaa agagtgaatc
    15781 atcgtgtacc tgttattgta ggggctggtt cgaactgtac gcaaacggcg atggaaaaaa
    15841 gtttaaagta tcagaatatg ggggcggatg ccttactatt aattagtcct tattataata
    15901 aggccaatac gcaaggtatg attcgtcact ttacaacggt cgccgatgcc gtacatattc
    15961 ctattatttt atataatgtg cctggacgta cagggtgtca tattgatgaa aaggcagtca
    16021 aagtattgag tcaacatcca aatatttgtg gaatcaaaga ggcgagtgga aatataagtt
    16081 atgccacaaa tattgcacgt tatctaagtg atgattttgt gatgtattca ggaaatgatg
    16141 acatgattac ttcgatgatg gcattaggtg ctagtggtgt cattagtgtt ttggctaatt
    16201 gcttacctca agaaacacat gaaatcgtgc aggattattt ggatggaaat gtgaaggcaa
    16261 gtctagaaaa acaattgaaa tatttagatt ttattcatgc cttatttata gaagtcaatc
    16321 caattccagt gaagcaggca ttgaattata tgggtaaaga tgttggtgga tatcgtttgc
    16381 ctttgtgtga aatggatgaa aagaatgcca aaattctaaa agctacaatg gaggatttgg
    16441 gtttatgtta aaaatcgcaa ttgtaggtac gggaaagatg ggttcgcttt taaaggaaac
    16501 cgcacaaaat caaggtatgg aagtggttca ttgttttgag aaaggaattg atgatacatc
    16561 tattgatgta gatgttgtga ttgatttttc acatcccaat aatttagcga gtgtcttaaa
    16621 ttttgtgaag attaaaaaat gtgctttagt ttatggaaca acgggattaa gtgagaatca
    16681 aatcaatagc ttaaaacaag tttcagatga aatcccttta ttttattcgg caaacttttc
    16741 atatggtatt gccgtatttg aacagattct aaaacaattt gcgcctttat taaagaatga
    16801 ttttgatatg gaagtacagg agattcatca taatcaaaag caggatgcac caagtggtac
    16861 tgctaaaatg ttggtgaatg ctttagatcc agaagatgaa tatacaaagg tatatggcag
    16921 aagtggtttt gtaggaaaac gccaacatga aattggaatt cattcacttc gtggtggaac
    16981 tgtggcaggt gagcatacag tgtatttctt tggagataat gaaacgttag aaatcaagca
    17041 tagtgcaaat tctagacaga tttttgtgaa tggggcttta aaagctgcac aatttgtagt
    17101 aaatcaaaag ccaggcttat atagtatgga agatatgatg taggaggtat atatggaagc
    17161 agaacaaatt attcgttata ttcaaatggc cgaaaagaaa acgccggtaa aggtttattt
    17221 taaatcagaa agagaaattt cttttccaaa ttgtaaagtc tttggcaacg ttgtatttgg
    17281 ggattggaaa gatattgaac cagtactttt aatgaatcca gaagtggact atgaagtcga
    17341 aaacaattgt cgtaatagtg ctgttcctat gttgaatcta aaaaatattc atgcacgtat
    17401 tgaaccaggt gcatttattc gtgaacaagt tgaaattggg gataatgccg ttattatgat
    17461 gggtgctatt attaatattg gtgctgtgat tggtgaaggt acaatgattg atatgggagc
    17521 cgttttaggt ggaagagcta ccgtaggaaa acattgtcat attggagctg gagctgtact
    17581 tgcaggtgtc attgaaccag ctagtgcaac acctgtcatt attgaagatg atgttttagt
    17641 tggtgccaat gccgtagtga ttgaaggggt tcatgtcggt aaaaatgccg ttgtagcagc
    17701 gggtgccgta gttattgatg atgtgcctga aaatgcggta gttgcaggat gtcctgcaag
    17761 agtcattaag atgaaggatg aaaaggctac gcaaaagaca aagttagagg atgctttaag
    17821 gaatctttag ggattccttt ttgtttgcca ttactaaaaa gtgtgcattt gcacagaaaa
    17881 tgggaatgga ttaattgtaa ttgagtgaaa atataatggg atatttgcgg attcgataaa
    17941 ttgtttttga tttatacatc tatttttcga atgattaaaa agaatccgaa atggttttaa
    18001 ataaagctga tttgcacttt ttgcgtagat gtataaatgt tacgaagctg atatattaaa
    18061 acatgaatca tgagaatata tatgcatgta aaataggcat ggatattgtt atttaaactg
    18121 tattttgata aaataacatt actaaaaagt gtgcatttgc acggaaatgg ggaatgggcc
    18181 tatggaaatt aaacgagatg cttacctaca acaattgatt gagcgaagag ataatggaat
    18241 gataaaggtg attaccggta ttcgtagatg tggtaaatcc tttttgttgt ttaacatttt
    18301 taaaagatat ttattagaaa gcggtgttgg tgttgatcat attattgaaa tcgcattaga
    18361 tggtattgag aatgaagaat taagaaatcc caaagtatgt ttcgagtata tcaaaaatgc
    18421 tatgaaagat gatgggaagt attatcttct tttggatgaa gtacagttta tgccccggtt
    18481 tgaggaagta ctaaatagtc tactgcgcat gagcaatatt gatgtgtatg tgactggaag
    18541 taactcaaaa tttttgtcca aagatattgt tactgagttt cgtggcagag gtgacgaaat
    18601 cagaatctat ccactttctt ttgcagagtt ttattcagta tatgatggtg attatgatga
    18661 tgcttgggat gattacatgg tttacggagg attgccgcag gttgttggtt ttcagagtga
    18721 acgacagaaa acagattatc tgaagaacat ttttacaaac gtttatctaa aagatgttgt
    18781 tgaaagaaat aagattcagg gtgtagacga gattggtatc ctggtagatg tgcttgcatc
    18841 tacaattggt gctcaaacca acccttcaaa gattgcgaaa acttttgcga gcgagcgtca
    18901 aatgacttat gcgaataaaa caatttcata ccatattgat tgtttggcag acgcattttt
    18961 gatttccaag gcaagtcgtt acgatattaa gggaagaaag tatataggag caaatttaaa
    19021 atactatttt actgatttgg gactgagaaa tgctcgcctt aattttaggc agcaggaacc
    19081 aacccatatc atggagaata ttgtttataa tgaacttctg attcgagggt ataacgtgga
    19141 tgtaggtgtt gtggatgttt tcgataaaga taaagaaggc aaacgtatcc gcaagcagtt
    19201 agaggttgac tttgtagtga atcaaggcaa ccaaagatat tatattcagg tagcctacga
    19261 tatgacttca gaagagaaac aagttcagga gttcaaatct ttgcgcaata ttccagattc
    19321 tttcaaaaaa attgtcattg taaatggtag taagaaacct tggagaaacg atgaaggctt
    19381 tgtgattatg ggaatgaaat attttctttt gaatgcaggt agtttggaat tttaaagttt
    19441 taaacagtga tttatacatc tattttcaaa tgattaaaaa agtccaaaat ggttttaaat
    19501 aaagctaaat tggacttttt gtgtagatgt ataaatgtta aagaaattta tgtgttagaa
    19561 cataaattat taaaatatgt atatgcacat aaaataagat tactaaaaat cgtgatttgc
    19621 acgtaaatag ggagtgaact aaagaagatc aactggaatt gggtgaaaat ataatggtgt
    19681 tcagtgatat ttgtagatgt gataaatctt ttttttgatt tatacatcta tttttttata
    19741 gatgtataaa tattgtgatt gtttttatgt tgaaatgtta taacaattga tttataatag
    19801 aattgaatgt tataacaatt aatcaggagg caattatgga aattaaagac gcaaaagtat
    19861 ttcatgatta tttaaatggt gtaattgaag gtgaggatgt taagaagagt acaaaattgt
    19921 attcgaatgc taaaacattc tattcaagtg tggattcaag tatttcagac gatcaaatta
    19981 tgtatgaagt atattcctat gaacatgatg gtttgtgtta tggattgact gtgatgcagc
    20041 cagtatgcgt gaatggagaa tgtaatgtaa cacgaggaca tttccatgag aatttagatt
    20101 gtgatgaaat ctattgtggt atgggtggtg aaggtcttct tttgttgatg gatgaagatt
    20161 atcattgtca tgcagaaaaa gtaaaatgtg gaagtgtaca ctatattgat gggcatttgg
    20221 cacatcgcct cgtgaataca ggtaatacgg aattaaaagt gcaatgtatg tggctaagtc
    20281 atgcgggtca tgattataag cgtgtagaag agcatccttt tacagtaaga gtgtttaaga
    20341 aaaatggtga attgaaggtg gaggaagaat gagttatcta aatttcaaat cggaatatga
    20401 tcgtttccct aaaacaaaaa taaagaatca taaagcgttt tgtggatatg aagaaatcta
    20461 tcgtgaatta aagagtaaaa tgaataataa cagtgttgta gtctttgatt attatccggg
    20521 tgtagatgaa aaggaagtct atgaattaat taaacgattc aatttttctt tagaaatcaa
    20581 tatgcatgat atctttaaag atggcaaaac catgaccgaa caaatgaaat ataatcttac
    20641 ggatgatcgt gtgtttggta agatgtatta tggtaatctt gtggatttta tagatgaaga
    20701 aaaactagat gaagctcgaa acaaaattaa agaacataaa gaatctgtca ttgtatatgg
    20761 agtgggtgct ggtttagtaa gtcatggtga tgtgtatgtt tactttgata tggctagatg
    20821 ggaaattcaa ctacgctatc gtaaagggat ggctaactat aatgcgacaa actttgatga
    20881 agatatctta aagaaatata agcgtggcta ttttattgaa tggcgtatag cggataagca
    20941 taaggctgag cgttttgatt gttttgatta tgtggtggat acaaatgttg cgaatgatcc
    21001 aaaaatgatt tcaagatata catttacaga aagtttaaaa caattagctc aaaaaccatt
    21061 tcgtacagta ccatactttg atccaggtgt atggggcggt caatggatga aggaagtttg
    21121 taacttagat gtgaatcagc caaattatgc atggagtttt gatggtgtgc ctgaagaaaa
    21181 cagcattcta tttgattatg atggagttac tgtagagtta ccagctatgg atttagtgtt
    21241 gtatcaacca aaagaattat tgggagaaca agtgtattct cgttttggtg cagaatttcc
    21301 aattcgtttt gactttctag atacgatgga aggacaaaac ttatctttgc aggtgcatcc
    21361 attaacggaa tatattaaaa agaattttgg catgagttat acacaagatg agagttatta
    21421 tattttagat gccaaagaag atgcccatgt ttatttaggt ctaaaagaag gcattcaacc
    21481 caatgaaatg atttctgatt tagagtctgc gcaacgtggt gaaattgtat ttgatgcgga
    21541 taaatatatc aataagtttc cggcaaagaa acatgatcat ttcttgattc cagcaggaac
    21601 ttgtcattgt tcaggaaaga atgcgatggt attagaaatt agtgctacac cttatatttt
    21661 tacgtttaaa ttgtgggact ggcaaagact tggcttagat ggcctacctc gacctatcaa
    21721 tattgaacat ggaaaacatg ttattcaatg ggatcgtacg acaaaatggg tcaaagaaaa
    21781 cttggtcaat gcgacttatg aagtaaaaga agatggaaat gattgcaaga tcgaacatac
    21841 gggtttacat gaactagaat ttattgaaac tagaagatat acaataaaaa acagttcata
    21901 tcagcaaaca catggaacag tgaatatgtt gaatttagtc gatggaaaga gtgcattaat
    21961 tgaaggggat tttgaacctt tcattgtgca ttatgcagaa acctttatta ttcctgcaag
    22021 tataaaagaa tatacaatca agccttatgg ttgtgatgaa gtgaaagtat taaaggctta
    22081 tgtgagattt taaagtaaaa gacggttttg tgcctaaaga aaaaacctga gagtgtatga
    22141 tctctcaggt tttcttagcc ttttaaggca tctaattctt cttgagttaa tgctctaaat
    22201 tcacctaatg gtaaatcatt gagttttaaa ggaccaaact caatacgttg aagataggta
    22261 actgtacaat ttgccttttc aaacattctt ttgacttggt ggaattttcc ttcatgaatc
    22321 gttagatagg ctttgtttgt atctaaaatt tctagttttg aaggggctga agtaaattct
    22381 tcaaattcca taggctgatt aaagaagtct acggcatttt ctggtagagt tccatcaaat
    22441 tcaacataat attttttatc tacatgtttt ttaggagata atacttcatg agttaacttt
    22501 ccatcattgc tgatgagtaa caagccttct gtatctacgt caagtcttcc cacaggatat
    22561 agatcgtgtc tttgactttg aataagttcc atgacagtcg gatagagcat atcttctgtg
    22621 gcgctcacat agccttgtgg tttattcaaa atgtaatatt cgaattctac atagcttacg
    22681 ggttcatcat ctacgcagac tctatcatcg tcttttacgt tatagtctgc tttttttatg
    22741 gtttcatcat taatagtaac ccagccattt ttaattaatt ttttaacgtc atttcttgtg
    22801 ccaaatccca tatgagataa atacttgtca agtctcattg ataacgccat cctttcgcat
    22861 atttgttttt taatgagcct ttagaaagtt tcgcaaaacc taaaggaaag ttatttacac
    22921 aaacaagtgt ccatccagat gtttttgaat ctaaatcctt tacatctaga gtttcacctt
    22981 ttaagtattt aataacacgc tcatcttcaa tggaaaagtt taatatattt ttaaattgtt
    23041 taggacaaag ggcaagggca agactttggc taggttcaaa atgttttcct tttaattgac
    23101 ctaataataa gccagatctt agtacgcgat agcctttata agcttgaaaa tctgtattaa
    23161 gccaaatgac tttgtctttt tttactttaa tggttccttt atttaaatga acgagtttta
    23221 aaaaatcagt aacacaatca tctaatttat cgcttggctc cagccttact tttttgtgtt
    23281 ttgtttctcc atctttgaca agtaaagcga caaagtgacc ttctcccttg attttatgtg
    23341 gatataaacg aacgcattct tccatgttta tacctggcat cataccatcg attttattaa
    23401 taggaacaag atgcaagtct ggatgtttgt ctaatacatc ttgaatgact tcttcatttt
    23461 catgaatcga aaaagtacaa gtggaatata cgatggatcc accaggcttt aacatagaga
    23521 tggctgcatt taaaatattt ttttgaatcg gaggatagaa tgtatcatcc ttttcaatcc
    23581 aacttttaat taaatctggt tctttacgaa acattccttc accactacat ggggcatcca
    23641 ctagaatctt atcaaatgtt tctgggtagt gttgttccat atttgtcaaa tcttcactgg
    23701 ttacccaggc gttgtcacaa ccaaataatt cgatgttttt taagaggcct tggcatcttg
    23761 aagaactgat atcattggaa attaaaagtc ccgtatgatt gagttttgtg gcaagctttg
    23821 tgctttttcc accaggagct gcacacgcat ctaatacaat atcattttcg tctacaggaa
    23881 gaacttgtgc tggtaacata gcacttggtt cttgaagata gtaaaggcct gcataatagt
    23941 aggggtgttt taaactggtt caaaagtcaa ggacaaatat ctgacagttc ttgttaaatg
    24001 agtgtcacaa atagtgatgc tcatt
//
LOCUS       DBMDWL010000010        23340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_809_length_23340_cov_3.882439, whole genome shotgun sequence.
ACCESSION   DBMDWL010000010 DBMDWL010000000
VERSION     DBMDWL010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 23340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23340
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_23340_cov_3.882439"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="ACZPDN_01325"
     CDS             complement(<1..508)
                     /locus_tag="ACZPDN_01325"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR1297297.1"
                     /translation="MKRCTWCNLSNPKYIEYHDYEWGKLNTDEKYLFEMLILESFQAG
                     LSWQCVLNKREAFKEAYEDFDIDKVIEFDEEKIKEASQEIVNGNLVIFPTETVYGIGA
                     NALNDKACKNIFKAKGREGDNPLIVHVNNVDMIKQLVEEPNEVEKILINSFCPGPFTL
                     ILKAKNIIP"
     gene            598..1542
                     /gene="moaA"
                     /locus_tag="ACZPDN_01330"
     CDS             598..1542
                     /gene="moaA"
                     /locus_tag="ACZPDN_01330"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR1297298.1"
                     /translation="MQDSFGRNINYMRISLTDRCNYACYYCQPDISKHLCHSDILTYE
                     QILDICRCAITLGITRFKITGGEPTLRKGYIDFIRRLKELDGVEQVTLTTNGSLFTFT
                     DLDELKKIGLDCINFSIDTLDEAEYLKICKKNNLKKVLLNLEYAYQIGIGVKVNCVVD
                     NLFSFSRFESLLEMIKDKKIALRFIELMPLKNSDRNTKMNEFIEYVQKNYTLNAYDKK
                     LGNGPAHYYTIPEFQGYIGFIEALHNKFCQDCNRVRLSSVGRLKLCLFHMDGVDLKPY
                     LNNLEELEKVMREWIFKKPKEHHFEEEHSSTVMNEIGG"
     gene            1544..2020
                     /gene="moaC"
                     /locus_tag="ACZPDN_01335"
     CDS             1544..2020
                     /gene="moaC"
                     /locus_tag="ACZPDN_01335"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR1297299.1"
                     /translation="MELTHFDEHGNARMVDVSKKDITSRSACAVGSILVNKEIVEKIE
                     HSDMKKGDVLGVARIAGIMGVKKTSDLIPLCHPLMINKVNIDFEIDKVNCLIYAYCTV
                     KCNGKTGVEMEALTGVNICLLTIYDMCKAVSKDMEIKNVHLVSKSGGKSGDFVWKE"
     gene            2008..2943
                     /locus_tag="ACZPDN_01340"
     CDS             2008..2943
                     /locus_tag="ACZPDN_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HIR1297300.1"
                     /translation="MEGIIKAICISKQKGTEKHPVQDIEIIEDHGLEKDAHAGKWHRQ
                     VSLLSYEKREEFKAKGANVEDGAFGENLLVSGIEFTSYPVGTQFKMGDVLLELTQIGK
                     SCHSHCTIYHQVGQCIMPHQGVFTRVIHGGHICVGDTVEVIENKDSRMRVAVLTLSDK
                     ASIGQRKDESGPLIEKIMTEHGYNVTSVTVIADDENEIVSQLINLSDRCQNDLILTTG
                     GTGLSVRDVTPEATMKVMTRNVPGISEALRYESMKYTNKAMLSRGVSVLRNQTLIINL
                     PGSPKAVKESLDIILGPLKHGLEIMKGEASECARK"
     gene            2924..3619
                     /gene="modB"
                     /locus_tag="ACZPDN_01345"
     CDS             2924..3619
                     /gene="modB"
                     /locus_tag="ACZPDN_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR1297301.1"
                     /translation="MSAQGSNLDLTPIWISLKTASITIVITFILGLLAAWWIYSRKSE
                     TLKMIFDGIFTLPMVLPPTVAGFFLLMIFGVNQPIGKFFMNVFGVRIAFSFSATVIAA
                     VVISFPLMYRNSRGAFEQVDTDLVYAARTLGMSEWNIFLKVLIPNALPGIISGGVLAF
                     ARGLGEFGATAMLAGNIIGKTRTLPVAVYSEVMSGDIESAAYYVWVIIGICFTAVILL
                     NLYQLYVRKKRSF"
     gene            3620..4252
                     /locus_tag="ACZPDN_01350"
     CDS             3620..4252
                     /locus_tag="ACZPDN_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR1297302.1"
                     /translation="MLEVQIYKKLAEFDLGASFQVNDNILGLMGASGSGKSMTLKCIA
                     GIETPDQGRIVLNNRVLFDSEKKINVPIQKRNVGYMFQSYALFPNMNVYENISVGLRA
                     RKVKDVDIVVQKVMQQFRVFELASRYPKQLSGGQRQRVALARLMAYEPDVLLLDEPFS
                     ALDEDLKEDLLQELKSELQISKPVIFVSHDKEEVNELCDLKYKIKQGEII"
     gene            4249..5025
                     /gene="modA"
                     /locus_tag="ACZPDN_01355"
     CDS             4249..5025
                     /gene="modA"
                     /locus_tag="ACZPDN_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1297303.1"
                     /translation="MKKGLSILLVGVMVTSLFGCSFNEPKEQVKLTIAAAASLENAFE
                     DELIPMFEKEYPNVTIEGVYDSSGKLQLQIEKGLDADVFFSAATTQMNALKDEDLVDA
                     DSISNVLENKLVLIKGKDSTTTVTGFDNIGDASIIAIGDPEVVPAGSYAKEALTNLGV
                     WDFIRDRLSLAGNVTEVLSQVETQNVEIGLVYATDAASSDKVEVVAECDNSLLATPVL
                     YPVGRVSSTKHKDEANSLIEFLKSDKALKIFKKYGFKKGE"
     gene            5027..6040
                     /locus_tag="ACZPDN_01360"
     CDS             5027..6040
                     /locus_tag="ACZPDN_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR1297304.1"
                     /translation="MKLIKTQDAVGHVLCHDITQIIKGVTKDALFRKGHIVTEEDIPL
                     LLSVGKEHLYVWENDESMMHENDAAMVLLDMCKNDYMSYGEIKEGKIELKSEIHGLFK
                     VDRKALFTVNSFGQMMIATRHSNTVVNPGDKLAGMRIIPLVIEKEKMDKVRSLITSSI
                     LEIKPFVLKKAAVITTGSEVYKGRIQDTFTPVIQAKLAEFGCEMVYHAICDDDDKMVT
                     EKINEALKQGVDIVLCTGGMSVDPDDRTPLAIKNTGANIISYGSPVLPGAMFLLAYKG
                     EVPIVGLPGCVMYAKRTVFDLILCRLLAKDAVTYEELCDLGEGGLCLGCDICTYPNCG
                     FGK"
     gene            6037..6369
                     /locus_tag="ACZPDN_01365"
     CDS             6037..6369
                     /locus_tag="ACZPDN_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1297305.1"
                     /translation="MRYKLKIQIGNNEPNFGKGVVELLELCDKYGSLSKAYKEMGMSN
                     SKAWKIIKRAQEDLGYELVSTTSGGSHGGGSCLTLEGKELVRKYKLFQEKIEIYSKQV
                     FKEVFEDE"
     gene            6362..6583
                     /locus_tag="ACZPDN_01370"
     CDS             6362..6583
                     /locus_tag="ACZPDN_01370"
                     /inference="COORDINATES: protein motif:HMM:NF023251.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HIR1297306.1"
                     /translation="MNKEEVLIITFKSIQDVLDLESKTKNGRMIPVPSCVKAGCGMCY
                     MSKDLDEEKWKLFLEEQNVLYEGIVRVNF"
     gene            6583..7599
                     /gene="selD"
                     /locus_tag="ACZPDN_01375"
     CDS             6583..7599
                     /gene="selD"
                     /locus_tag="ACZPDN_01375"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR1297307.1"
                     /translation="MNEKIYCKSGGCSAKLGAKKLSHILEKIDSYKDSHVLVGFDSRD
                     DAAVYQIDENHAFVSTLDFFPPMVEDPYLYGQIAATNALSDIYAMNGTPKTALNIVCF
                     PENEDLNILGKIIEGGNSKLVEAKCALVGGHSIRDDSIKYGLSVTGLVNPIDVKKNNG
                     TQCGDVLVLTKPLGVGLTLNALRMDDCSKSMQERVFKSMTTLNKYACDILKKYDVHAL
                     TDVTGFGLLVHLNEMLDEKNSAVIYKDSIPYFDDCQKYVEEFYLSGAAQTNRNSVEGK
                     ISFDCDFFTEEILFDPQTSGGLLASVSKADLEMLEKDFKENNLELYVVGEVIEKNEYN
                     VYVR"
     gene            7606..8199
                     /gene="yedF"
                     /locus_tag="ACZPDN_01380"
     CDS             7606..8199
                     /gene="yedF"
                     /locus_tag="ACZPDN_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HIR1297308.1"
                     /translation="MKTLDCRGLECPLPVVKTKEALKEENVVSTIVDNEIAVENLTKF
                     AKVKNYTVNSKKEGKDYIVEISKGDEEADFEVEYTYADCSLAKPKTVVVFASNVMGSN
                     PDLGAILMKSFIFSLTKQDVLPDEMIFYNEGVKITTTKNETLEDLKYLANNGVEIVSC
                     GTCLDFFHLKEELQVGSVTNMYDIVERMEKADKIIRP"
     gene            8205..8996
                     /gene="yqeB"
                     /locus_tag="ACZPDN_01385"
     CDS             8205..8996
                     /gene="yqeB"
                     /locus_tag="ACZPDN_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR1297309.1"
                     /translation="MNKCVIVRGGGDIATGTIYKLVKSGFQVLVLEVEKPSAIRRNVA
                     FCQAVYEQKFTVEDMTCVLVSSIEEAKKVMSKNQVAMMIDPSGEMISKIEHIALVDAI
                     LAKKNLGTTIDMAPITIGLGPGFCAGKDVHVVVETMRGHNLGRLIYQGYALPNTGVPG
                     NIKGYSKERVIHSPCAGVCHNVKKITDIVDKGEIIAYIDETPVYASMSGLLRGLIQDG
                     YNVTSGFKMADIDPRVDEYQNCFTISDKARCIGGGVLEAILHGLS"
     gene            8983..10074
                     /locus_tag="ACZPDN_01390"
     CDS             8983..10074
                     /locus_tag="ACZPDN_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1297310.1"
                     /translation="MVYLDQAATSFPKLEEVKQAVMRAFDVAGNANRSSNMDSSRLIF
                     EARRNIAQFFGLSDFHRVILNGGNTESLNTCIKGILKEKDHVITTYAEHNSVLRPLYE
                     LEEKGIITLTICAPYLENIKNHIQKNTRMVIMTHSSNVTGEIFDVDLIGQYCKECGVL
                     FMVDAAQSAGHIPVSMENIDVLCFSGHKGLLGIGGIGGFCVKDMEVKPLISGGTGIDS
                     FNPQMPEAYPEHLEAGTQNIVGIAALNAGIQYVQSHQKEIVEKECFLLQKLYEGLSGY
                     VDFYSSLENSTPIVAINIPGKDSAYVSDLLNYKYGIVTRCGAHCAPLMHKHLKTEERG
                     IVRLSISFQNTIEDVDFVVRAIQKISNDY"
     gene            10064..11431
                     /gene="yqeC"
                     /locus_tag="ACZPDN_01395"
     CDS             10064..11431
                     /gene="yqeC"
                     /locus_tag="ACZPDN_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR1297311.1"
                     /translation="MIISVIGSGGKTTYIHELKDKYVSLNKTVLMCTTTHMLIEEDTL
                     VDPGYDEIMQRIETYGYCHAGNRCGDLKIEALDEELLNQLKQVVDVILIEADGSKHLP
                     LKFPSANEPVIDSDTDEVVLISNLNGLNQPVKDVVHRYELANLDPSELVTPRIMQDLI
                     CAYLKKLNKPVKIHVNGASDLYTRCVKALLEENMDVNLIQKEWFNMQPKLVILGCGHV
                     SQYLAKMASILELYTIVIDNRKEFANRECFPTANEIHCIDYDQMDSVLPDEENACYVI
                     VTRGHKDDRLCLEKTIHRPHLYLGMIGSKGKVKKTFDALIEEGYLKEELSNVHAPIGL
                     DIKAQTPAEISISILAELIEIKNTKFSSSVSKELLESNMHGTLCIIIDKKGSAPRGVG
                     SMMLVHKDGVIDTIGGGKVEYQAILDAKECKEVMIKEYDLSNAESATLGMICGGYNKV
                     LFIPV"
     gene            11446..12240
                     /locus_tag="ACZPDN_01400"
     CDS             11446..12240
                     /locus_tag="ACZPDN_01400"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF014600.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1297312.1"
                     /translation="MKKRRRRVRKSVIFVIVVLLLIIALLISMIAKKVSDKEVSEPKQ
                     EKTTETKKENKKQQTNLPKVKKINQSGDPYENTVTVSDLDSIYILVNKYSGLPADYEP
                     SDLVQVPSSGENENVRMRKEAAEAFEKLIEAASNEGFILNACSAYRSYAYQTDLFNRG
                     AASNGEEYADRYWTRPGYSEHQSGLAVDIRMDNDCSDLDAVRNNSHYDWLLENMHMYG
                     FILRYPDDKEDKTKIAPESWHLRYVGQDLATYLYKNNLALDEYYGA"
     gene            12320..12820
                     /locus_tag="ACZPDN_01405"
     CDS             12320..12820
                     /locus_tag="ACZPDN_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019886.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1297313.1"
                     /translation="MNIDKKIIVGVRNKDANAKKELISLTRNHLIQTIYKRYIGLEEK
                     EINDILQATYTYAFMHMDKLSDDKNFLKWITVLLNKQLREYMQAKEHADVTTALRGLP
                     QYRQSDVDDCLNEMPENQKTVTIMHCLGKLKEKDIANRLKMSEEGVARLLKSGKQKVK
                     DVLEKK"
     gene            12967..13968
                     /locus_tag="ACZPDN_01410"
     CDS             12967..13968
                     /locus_tag="ACZPDN_01410"
                     /inference="COORDINATES: protein motif:HMM:NF023156.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HIR1297314.1"
                     /translation="MKRDTKRVLILSLKIGVGSAIAIILAYFFKLDNPTSAGTIALLS
                     LLTTKWGTMRLVLRRISTFFVTILFCFIFFELIPSHWIAFGLVIACLVGYSEKMKCQN
                     TLSVNAMIAVHYLSYLDFSLHFMMNEFYLILIGAIIAFLLNLVHDYSGEEEYLNSCMI
                     YMEDKIQSLMYQIVHYIQSEERNTTIWKELEDVKEQAEKYIHIAMEYQDNTFTNLPDY
                     YIRYFEMRALQCDILHMLHYKIRKIRKMPKEANELANYIEYLIPFIHEKNDPQPQITS
                     LHQMFKNKQGEALPKSRIEFESKAMLLHIYMDLEEFLYTKKKFIDQTTEEQKKLYWR"
     gene            13974..14744
                     /locus_tag="ACZPDN_01415"
     CDS             13974..14744
                     /locus_tag="ACZPDN_01415"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1297315.1"
                     /translation="MKSIIIVTVVSIIVSIGIYKGIRKVLYKKDVTNKASFARLIKYI
                     LVFINGMIFLGQFEQTKPIMSYLAASSGVVALVLGVAAKDAFGNLCSGLMILTFKPFV
                     EGDLIKVNQGELIGYVESIRFRHTIIRTYESNRIIVPNATLNSATIENAFFQDTRKNN
                     YLEIEVSYGTDIDLAIDILKSLVEKTMIEYEIQSEDPIEVKVINFSTTGIDLRVVVPS
                     KTSLEGFDMLAKIRYELIKEYEKANIHFGNYSVEVSQS"
     gene            14741..15286
                     /locus_tag="ACZPDN_01420"
     CDS             14741..15286
                     /locus_tag="ACZPDN_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297316.1"
                     /translation="MIKVYTEVLGFNNLMYRYMSLPNSLSLADLAYYTLASYLSEEYA
                     DFVIESNGIPFACPNCEDEQFNKLPCASSIQPDFKEGDTFKMVYDLEEPFEIEIQVEK
                     VDDIEEDANVVNGRGFAIWDGFKEFQDYYYQSMELFKEYVKANDMNLSDFPVDEDLDI
                     IELNKVLEDNFEELKDVYEGE"
     gene            15287..15904
                     /locus_tag="ACZPDN_01425"
     CDS             15287..15904
                     /locus_tag="ACZPDN_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297317.1"
                     /translation="MKVYEMSFRDMDQKMVEIHGVKMVKLLEKMGLKLDNLYGALMYG
                     YIDHNAGFIFEIVALETKKRNIEYRIVPIGVSCKIPRFDVQEMDIQILDNVNVELFQD
                     KIDMVEKATEVSKELEELRLYKELDSSRHLEYPDDIMVYFLEEGKDVEACWVRLEGIQ
                     DGKMYGSVLTALHQDFGVKERDTIYFGMTEMEDHKLACVWVKEDE"
     gene            15897..17171
                     /locus_tag="ACZPDN_01430"
     CDS             15897..17171
                     /locus_tag="ACZPDN_01430"
                     /EC_number="4.4.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0080146 - L-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cysteine desulfidase family protein"
                     /protein_id="HIR1297318.1"
                     /translation="MNKEDSLYSIYVQILKEELVMAMGCTEPIAISYACAKATQVLDH
                     LPDRIVVKASGSIIKNVKSVIVPHTNGLKGIEVAAAAGALYGDADAKLEVLSSATREQ
                     IEELPEYVQNTNITVQHIEQGHVFDLEINVYYEQEHASVRIVDTHTNIVQIEKDWKVV
                     FEDKTTSLEHKADHSALIMKQIWDFAKTVDIEDVKEILDRQIACNMAIANEGIHNSYG
                     ANIGHVILNMDLDCVKTRAKAYAAAGSDARMNGCELPVVINSGSGNQGITCCVPVVVY
                     AKELNSTQEQLYRALVLSNLTAIYIKTGIGTLSAFCGAVSAGAAAGAGIAYLHDGTYE
                     EIQHTIVNALAILSGTICDGAKASCAAKIASSIDAGIMGYYMYKNKQQFYAGDGIVAH
                     SVDETIQNIGTLGSQGMLQTNDKIIEMMISCD"
     gene            complement(17143..18174)
                     /locus_tag="ACZPDN_01435"
     CDS             complement(17143..18174)
                     /locus_tag="ACZPDN_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1297319.1"
                     /translation="MGKRNKVTTRDIAKAINISQSTVSMILSDNKNVSFKEETVKLVK
                     KAAKQMGYKKPEVKKRYSNTKNTIVVICSNLSNSYYSMVVSSIIQQANKTKTQILTIS
                     TLRKDELEKHALSSLKLLKPCGIILLYPPKFVDEWNALSKQIPCIIIGDKPKNLDCDS
                     IELNSFKCGNLLGNHLKQLGHKKIAYISSPLDTTEIGRNERYEGVKSVISNTQVFFSS
                     SKQFDMYPIEKREYENGYRLTKQILGDKYDAYIGNNDMTALGILACLKDHNIKTSVAG
                     FDNIPETDFPQIQLTTVDHGACEKGKEAVDILLEQTKSERKRIVHMEYEPQLIIRLST
                     FNRKKSSSQ"
     gene            18373..19803
                     /locus_tag="ACZPDN_01440"
     CDS             18373..19803
                     /locus_tag="ACZPDN_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1297320.1"
                     /translation="MYDCIIIGAGISGCSLAFELSKYSGKVLWLEKNNDVADETTKAN
                     SAIIHAGYDPEPNTLMAKYNIWGNKIIPSLCKDLDVPCKNIGSLVVGFSEEDDKTIEV
                     LYKRGIENGVENQRIIKQPELSKMEPNLNPACTSALYAPSACIINPWQFAIALSEVAI
                     QNGVELRLNEEVTSIKKDGDIFIVNDTYKAKLVVNCAGVMCDHVTGLIKKPNYKITPN
                     KGEYYLLDKSQGNLVHHVIFQCPTKIGKGVLVAPTVDGNLIVGPTSTNTDVDDYSTSA
                     AGLKTIRENAVKSVPNMKLQENIRTFAGLRARSDKKDFIVGEDEDVHNYFSIAGMASP
                     GLSSASAIALDVVRMMQEKNYFLNLKKDYVSTRKVVRFKEMSHEQRKEYIEKHPEYGR
                     IICRCETISEGEIIDAIRRSPKPVSLDGIKRRCNAGMGRCQSGFCGPRVQEILANELG
                     VSQLDIMLDKNGSYILCGRTKENDDE"
     gene            19796..21061
                     /locus_tag="ACZPDN_01445"
     CDS             19796..21061
                     /locus_tag="ACZPDN_01445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1297321.1"
                     /translation="MNNKYDVVVIGGGPAGLASAMAAKQNGAKSVLIIERDKELGGIL
                     NQCIHNGFGLHHFKEELTGPEYAGKYIDEVNKTDIDILLDTMVIEITPDKMVYCSSSI
                     HGYLMIQAKSIILNMGCRERTRGAIAIPGTRASGVFTAGAAQRYVNIEGYMPGKRVVI
                     LGSGDIGLIMARRMTLEGAKVLAVVEVMPYSNGLNRNIVQCLHDYDIPLYLSHTITDI
                     VGKSRVEKVVVSKVDENRNPIEGTEMEFDCDCVLLSVGLIPENELSNDLGVEMDSRTH
                     GPIVYENMETSMEGVFASGNVVHVHDLVDFVSLESEKAGMGAGRYVSNGEVSKNCVVK
                     TINGDNISYVVPQYIRKDNVEKSVELSFRVRRPAQRVNIVVKDGDTVIAKFKKEHVIP
                     SEMEKISIPKVLLEKADKELVVSVKEDVQ"
     gene            21058..21402
                     /locus_tag="ACZPDN_01450"
     CDS             21058..21402
                     /locus_tag="ACZPDN_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR1297322.1"
                     /translation="MRELICITCPKGCHLKVDEENDYKVTGNGCPRGAIYGKKECTHP
                     TRVVTSTVKIVGAEIERLSVKTNGDIPKEKIFDVMKELNHVVVKAPVHREDVIVSDIC
                     GTGVDLVATKDL"
     regulatory      21408..21507
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            21555..22145
                     /locus_tag="ACZPDN_01455"
     CDS             21555..22145
                     /locus_tag="ACZPDN_01455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1297323.1"
                     /translation="MNYFIFDVDGTILDSMEVWNTLASRYVQSLGMIPQNNLDEIVSN
                     MSLEQSATYLKKHYGINKSEERIISEVLNLISDFYKYEVKLMPGFKDFISHYDSVNVI
                     GTSCDEKLVKIALNRLGVLNNFEDIITCSKVNKPKDDPNFYLACAQVLKQRPEDIVVF
                     EDADYCIDVARKIGFKVIKIKDWRDLNKKCINNCGK"
     gene            22186..22919
                     /gene="thiD"
                     /locus_tag="ACZPDN_01460"
                     /pseudo
     CDS             22186..22919
                     /gene="thiD"
                     /locus_tag="ACZPDN_01460"
                     /inference="COORDINATES: protein motif:HMM:NF020132.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
BASE COUNT         7808 a         2831 c         5196 g         7505 t
ORIGIN      
        1 ttggaattat atttttagct tttaatatta atgtaaaagg acctgggcaa aaagaattaa
       61 ttaatatttt ttctacttca tttggttctt ctacaagctg tttaatcata tctacattat
      121 ttacatgtac tattaacgga ttatcccctt ccctaccctt tgctttaaaa atatttttac
      181 aagccttatc atttaatgca tttgcaccta ttccatatac tgtttctgtc ggaaatatta
      241 ctaaattccc atttactatc tcttgtgatg cttcttttat tttttcttca tcaaattcaa
      301 tcactttatc aatgtcaaaa tcttcataag cctctttaaa agcttctctt ttatttagaa
      361 cacactgcca actaagacct gcctgaaaag attcaagtat caacatttca aatagatatt
      421 tttcatctgt atttagtttt ccccattcat aatcatgata ttcaatatat tttggattgc
      481 ttagattgca ccacgtacat cgtttcatga ttccatttta aagtagataa aatgtgaatt
      541 caatgataaa attgaatcag ttggttattc tcactcgaga ataacggaaa ggattttatg
      601 caagattcat ttggacgaaa tataaattat atgcgaatct cattaactga cagatgtaac
      661 tatgcttgtt actactgcca gccagacatc tcaaaacatc tttgtcattc ggatatattg
      721 acgtatgaac aaattttaga tatttgtaga tgtgccatta ctttgggtat tactcgattt
      781 aaaatcacgg gtggagaacc cacacttcgt aaaggttata ttgattttat tcgtagattg
      841 aaagaattag atggtgttga acaagtaact ctcacaacca atggaagttt atttactttt
      901 acagatttag atgaactaaa aaaaataggg cttgactgta ttaacttttc aatcgataca
      961 cttgatgaag cggaatattt gaaaatatgt aaaaagaaca atttgaagaa ggttttgttg
     1021 aatttagaat atgcctatca gattggtatt ggggtgaaag taaattgtgt tgtggacaat
     1081 ctattttcat tttctcgatt tgaatctttg ttagaaatga ttaaagataa aaaaattgcg
     1141 ttacgcttta ttgaattgat gccattaaaa aatagtgatc gaaacacaaa aatgaatgaa
     1201 ttcattgaat atgtgcaaaa gaattatacg ttaaatgcgt atgataaaaa gttaggtaat
     1261 ggaccagccc actattatac aataccagag tttcagggat acattggttt tattgaagct
     1321 ttacataata aattttgtca agactgcaat cgtgtacgct taagcagtgt aggacgcttg
     1381 aaattatgtt tattccatat ggatggtgtg gatttaaaac cttatctaaa taatttggaa
     1441 gaactagaaa aagtgatgag agaatggata ttcaaaaagc ccaaggaaca tcattttgaa
     1501 gaagaacatt catcgacggt gatgaatgaa attggaggat aaaatggaat taacacattt
     1561 tgatgaacat ggaaatgcac gtatggtgga tgtaagcaag aaggatatta cttctagaag
     1621 tgcttgtgcc gttggaagta tattagtaaa taaagagatt gttgaaaaaa tagaacatag
     1681 tgatatgaaa aaaggagatg tacttggtgt agctcgtatt gccggaatca tgggcgtaaa
     1741 aaagacgagt gatttgattc ctttgtgtca tcctttaatg atcaataagg tcaatattga
     1801 ttttgagatt gataaagtga attgtttgat ctatgcctat tgcacagtga agtgtaatgg
     1861 gaaaacaggt gtagaaatgg aagctttgac aggtgtaaat atttgtcttt tgacaattta
     1921 tgacatgtgt aaagctgtaa gtaaagatat ggaaataaaa aatgtgcatt tggtttctaa
     1981 atcaggagga aaaagcgggg attttgtatg gaaggaataa ttaaggcaat ttgtatcagc
     2041 aaacaaaagg gaacagaaaa acatccggtt caagatattg aaatcattga agatcatgga
     2101 ctagaaaagg atgcgcatgc aggtaaatgg catcgccaag tttctttatt gtcctatgaa
     2161 aagcgtgaag aatttaaggc taaaggtgcc aatgtagaag atggtgcctt tggagaaaat
     2221 ttacttgttt caggtattga attcacaagt tatcctgtag gaacacaatt taaaatgggg
     2281 gatgtattac tcgaactgac gcaaatcggt aaaagttgcc attctcattg taccatctat
     2341 catcaagtag gacagtgtat catgcctcat caaggtgtat ttacgcgtgt aattcatggt
     2401 ggacatatat gtgttgggga taccgttgaa gtcattgaaa ataaagattc tagaatgcgt
     2461 gtagccgtat tgacattaag tgataaggca agtatagggc aaagaaaaga tgagagtggt
     2521 ccattgatcg aaaagattat gacagaacat ggatacaatg tgacaagtgt gacagtgatt
     2581 gcggatgacg aaaatgaaat tgtttcgcaa ctcatcaatt taagtgatcg ttgccaaaac
     2641 gatttgattc ttacaaccgg aggcacagga cttagtgttc gtgatgtgac accagaagcc
     2701 acaatgaaag tcatgactag aaatgttcca ggtattagtg aagcattgcg ctatgaatcg
     2761 atgaagtata caaataaggc gatgttgtct cgtggtgtga gtgtattaag aaatcagacg
     2821 ttgattatca atttaccagg aagtcctaag gcagtaaagg aaagtctgga tattatttta
     2881 ggaccattaa aacatggctt agaaatcatg aaaggggaag cgagtgagtg cgcaaggaag
     2941 taatttggat ttaacgccaa tttggatatc tttaaaaacc gcaagtatca caattgtgat
     3001 tacttttatt cttggtttac ttgctgcatg gtggatttat tcaagaaaga gtgaaacttt
     3061 gaaaatgatc tttgatggaa tctttacttt gcctatggtt ttgccaccta cagtagctgg
     3121 ctttttcttg ttgatgattt ttggtgtaaa tcaacccata ggcaaattct ttatgaatgt
     3181 atttggtgtt cgcatcgcat ttagttttag tgcgactgtg attgcagctg tggtcatttc
     3241 atttccactc atgtatcgaa attctagagg tgcttttgaa caagtggaca cagatttagt
     3301 atatgccgca agaactttag gaatgagcga atggaacatt ttcttgaaag tattgatacc
     3361 caatgccttg ccaggaatta ttagtggtgg tgtacttgcg tttgcgcgtg gcttaggtga
     3421 gtttggggca acggcgatgc ttgcaggaaa catcatagga aaaactcgaa cacttcctgt
     3481 agctgtatat agtgaagtga tgagtgggga tatagaatcg gctgcctatt atgtatgggt
     3541 gatcattggt atttgtttta cagctgtgat cttgttgaat ctatatcagt tgtatgttcg
     3601 aaagaaaagg agtttctaaa tgttggaagt acaaatatat aagaaacttg cagagtttga
     3661 tttaggcgct tcctttcaag tgaacgacaa tattttaggg cttatgggcg cttcaggatc
     3721 tggtaaaagt atgacattaa aatgtatagc tggaattgaa acgccagatc aaggacggat
     3781 tgtcttgaat aatcgtgttt tatttgattc agaaaagaaa ataaatgtac cgattcaaaa
     3841 acgaaatgta ggctatatgt ttcaaagcta tgctctattt ccaaatatga atgtatatga
     3901 aaatattagt gtgggattaa gggctagaaa agtaaaagac gtggatatcg tggttcaaaa
     3961 agtgatgcag caatttcggg tttttgaact tgcttcaaga tatcccaaac aattatcggg
     4021 tggacaaaga caaagggtgg cattggctcg cttgatggct tatgaaccag atgttctttt
     4081 attggatgaa ccatttagtg ctttagatga agatttgaag gaagatttat tacaggaact
     4141 taaaagtgag cttcaaattt caaaaccggt tatttttgta tcgcacgata aagaggaagt
     4201 aaatgagttg tgtgatctaa aatataaaat taaacaagga gagatcatat gaaaaaaggt
     4261 ttaagtattt tattggttgg agttatggtc acaagtttat ttgggtgtag ttttaatgaa
     4321 ccaaaggaac aagtaaaact tacaattgcg gcagccgcaa gtttggaaaa cgcatttgaa
     4381 gatgaattga ttccaatgtt tgaaaaagaa tatccaaatg taacgattga aggggtctat
     4441 gatagtagtg gaaaattaca gcttcaaatt gaaaaaggat tagatgccga tgtatttttt
     4501 agtgcagcta caacacagat gaacgcatta aaagatgaag atttagtaga tgcggattca
     4561 atttcaaatg tattagagaa taaattggta ttgatcaaag gaaaagacag cactacaaca
     4621 gtcactggat ttgacaatat tggggatgct tccattattg cgattggaga tccagaagta
     4681 gttcctgcag gaagctacgc aaaggaagct ttgacaaact taggtgtatg ggattttatt
     4741 cgagatcgtt taagcttagc aggaaatgta acggaagtat tgtctcaagt tgaaacacaa
     4801 aatgtagaaa ttggtttagt ttacgcgaca gatgctgcaa gtagtgataa agtagaggta
     4861 gtggcagagt gcgataattc attattagct acaccagtac tatatccagt tggtcgtgta
     4921 tcgagtacaa agcataaaga tgaagccaat tcattgattg aattcttaaa gagcgataaa
     4981 gctttaaaaa tattcaaaaa atacggattt aaaaaaggag aataagatga aattaatcaa
     5041 gacacaggat gccgtagggc atgtactttg tcatgatata acacagatta ttaagggtgt
     5101 cacaaaagat gccctttttc gtaagggtca tatcgtgaca gaagaagata ttccattatt
     5161 attatcagtt ggaaaagaac atttatatgt ttgggaaaac gacgaaagta tgatgcatga
     5221 aaatgatgcg gctatggttt tgttagacat gtgcaagaat gattatatgt cgtatggtga
     5281 gatcaaagaa gggaaaattg aattaaaaag tgagattcac ggactattta aagtagatcg
     5341 taaggctttg tttacagtaa attcatttgg acagatgatg attgcgacta gacactcaaa
     5401 cacagtagta aatccgggag ataaacttgc gggtatgcgt attattcctt tagtaattga
     5461 aaaagaaaaa atggataaag ttagatcact catcacatct agtattcttg aaattaaacc
     5521 gtttgtatta aaaaaagcag ctgtaatcac aacaggaagt gaagtatata aaggaagaat
     5581 tcaagatacg tttacaccgg ttattcaagc taaattggct gagtttggtt gtgaaatggt
     5641 gtatcatgcg atttgtgatg atgacgataa aatggtgaca gaaaaaatca atgaagcttt
     5701 aaaacaaggc gtggacattg ttttatgtac gggtggaatg agtgtggatc cagatgatcg
     5761 aacaccactt gcaatcaaga atacaggggc gaatattatt agttatggtt ctccagttct
     5821 tcctggtgct atgtttttac ttgcctataa aggagaagtt ccaattgttg gtcttccagg
     5881 atgtgtgatg tatgccaaaa gaacggtatt tgatttaata ctttgtcgtt tgcttgccaa
     5941 agatgcagtg acatatgaag agttatgtga tttaggtgaa ggtggattgt gcttaggatg
     6001 tgatatttgc acatatccga attgtggatt tggaaaatga gatataaact aaaaattcaa
     6061 attggtaata acgaaccaaa ttttggtaaa ggcgttgtag aactattgga gttatgtgat
     6121 aagtacggtt ctctatcaaa ggcttataaa gaaatgggaa tgtcaaattc aaaggcttgg
     6181 aaaatcatca aaagagctca agaagattta gggtatgagt tggtttctac aacgagtggt
     6241 ggaagtcatg gtggaggctc ttgcttaact ttagagggaa aagaattggt acgtaaatat
     6301 aaattgtttc aagaaaaaat cgaaatatat tcaaaacaag tgtttaagga agtatttgaa
     6361 gatgaataaa gaggaagtat tgattattac ttttaaaagt attcaagatg ttctagattt
     6421 ggagtcaaag acaaaaaacg gacgtatgat acctgttcca agttgtgtta aggcaggatg
     6481 tggcatgtgt tatatgtcga aagatctaga tgaggaaaag tggaaacttt ttttagaaga
     6541 acaaaacgtt ttgtatgaag gtatagtaag ggtgaatttt taatgaatga aaaaatatat
     6601 tgtaagagtg ggggatgtag tgccaaattg ggcgctaaaa aattgagtca tattctagaa
     6661 aaaattgatt cttataaaga tagtcatgtc ttggttggct ttgattcaag agatgatgca
     6721 gctgtttatc aaatcgatga gaatcatgcg tttgtatcta ctttggattt ctttccacct
     6781 atggttgaag atccatattt gtatggtcaa attgcggcta caaatgcact cagtgatatt
     6841 tatgcgatga acggtacgcc taaaacagct ttaaatattg tatgttttcc agaaaatgaa
     6901 gatttgaata ttttaggtaa gattattgaa ggtggaaatt ctaagttagt tgaagctaag
     6961 tgtgctttgg taggtggaca ctctattcgt gatgattcca ttaaatatgg attgagtgta
     7021 acgggtttag tgaatccaat agatgtaaag aaaaataatg gtacgcaatg tggagatgtt
     7081 ttggttttga caaagccttt aggtgttggt ttgactttga atgcgttgcg tatggatgat
     7141 tgttcaaaat caatgcagga aagagttttt aaaagtatga ctacattaaa taagtatgcg
     7201 tgtgatattt taaaaaaata tgatgtgcat gctctaacgg atgtaacagg ctttggcttg
     7261 ttggtgcatt taaatgagat gttagacgaa aaaaatagtg cagtgattta taaagattcg
     7321 attccatatt ttgatgattg ccaaaagtat gtcgaagagt tttatttaag tggggcagca
     7381 caaaccaatc gcaatagtgt agagggtaag attagttttg attgtgattt ctttacagaa
     7441 gaaatcttgt ttgacccaca aacgagtggt ggcttattag ctagtgtatc taaagcggat
     7501 ttggaaatgc tagagaaaga ttttaaggaa aataatcttg aattgtatgt tgtgggtgaa
     7561 gtcattgaga aaaatgaata taatgtatat gtgaggtagt aagatatgaa aactttagat
     7621 tgtagaggat tggagtgtcc acttccagtt gtaaagacaa aggaagcttt aaaagaagaa
     7681 aatgtagttt caacaattgt ggataatgag attgcggttg aaaacttaac gaagtttgcg
     7741 aaagtaaaaa attatacggt aaattctaaa aaagagggta aggattatat tgttgaaatt
     7801 tcaaagggtg atgaagaagc tgattttgaa gtagaatata cttatgcaga ttgtagtttg
     7861 gctaagccta aaacggttgt agtgttcgcc agtaatgtga tgggttccaa tccggattta
     7921 ggtgcgattt taatgaaatc atttattttc tctttgacaa aacaagatgt tttgccagat
     7981 gaaatgattt tctataatga gggtgttaaa atcacaacta ctaaaaatga gactttagaa
     8041 gatttaaaat atttagcgaa caatggagta gaaattgtaa gttgtggaac ttgtttagat
     8101 ttcttccatt taaaagaaga acttcaggtg ggttcagtca caaatatgta tgatattgta
     8161 gaaagaatgg agaaggctga taaaattatc aggccataag gattatgaac aaatgtgtta
     8221 ttgtgcgtgg tggaggagac atagcgacag ggacaatata taaacttgtg aaaagtggat
     8281 ttcaagtatt ggtgttagaa gttgaaaagc caagcgcaat acgaaggaat gttgcctttt
     8341 gtcaggctgt gtatgaacaa aagtttacgg ttgaagatat gacgtgtgtt ttggtttcat
     8401 cgatagaaga agctaaaaaa gtgatgtcta aaaatcaagt cgccatgatg attgatccta
     8461 gtggcgaaat gatttctaaa atagaacata ttgccttggt ggatgcgatt cttgccaaga
     8521 agaatttagg tacgacaata gatatggcac ccatcacaat tggcttagga cctggctttt
     8581 gtgcaggaaa agatgtacat gtggttgtag aaacgatgcg tgggcataat ctaggacgat
     8641 tgatctacca gggatatgct ttgccaaata cgggcgttcc tggaaatatt aagggatatt
     8701 caaaagaaag agtgattcat tctccttgtg caggtgtttg tcacaatgtc aaaaagatta
     8761 cagatatcgt tgataaagga gaaattattg cgtatattga tgagacaccc gtgtatgcga
     8821 gcatgtcagg cttacttaga ggattaattc aggatgggta caatgtaaca tctggattta
     8881 aaatggcaga tattgatcct agagtggatg aatatcagaa ttgttttaca atttctgaca
     8941 aggcacgttg tattggtgga ggcgttttgg aggcgatact gcatggttta tcttgatcag
     9001 gctgcgacta gctttcccaa attagaggaa gtcaaacaag ctgtaatgag ggcattcgat
     9061 gtggcaggca acgcgaatcg tagttcaaat atggattctt cacgtttgat ttttgaagca
     9121 agaagaaata ttgcacagtt ttttggcttg tctgattttc atcgtgttat tttaaatggt
     9181 ggaaataccg agagtttaaa tacttgcatt aaaggaatct taaaagaaaa agatcatgtg
     9241 attactacct atgcagagca caatagtgtg cttcgtcctt tatatgagtt agaggaaaaa
     9301 ggaataatca cattaacgat atgtgctcct tatttagaga atattaaaaa tcatattcaa
     9361 aagaatactc gaatggttat catgacacac agttccaatg tgacaggtga aatctttgat
     9421 gtggatttaa ttggacagta ttgtaaagaa tgtggtgtcc tatttatggt ggatgcagcg
     9481 caaagtgcag gtcatattcc tgtgtctatg gaaaatatag atgttttatg ttttagtggc
     9541 cataaaggtc tattagggat tggtggtatt ggtggctttt gtgtaaaaga catggaagta
     9601 aaaccattga tttctggtgg aacaggtata gattctttta atccacaaat gcctgaggca
     9661 tatccagaac atttagaggc gggtacgcaa aatattgtag ggattgcggc tttaaatgca
     9721 ggaattcaat atgttcaatc tcatcaaaaa gagattgtgg aaaaggaatg ttttttactt
     9781 caaaaattat atgaaggttt atctggctat gtagattttt attcatcttt agaaaattcg
     9841 acacctattg tggcaataaa tattccaggt aaagatagtg cttatgtgag cgatttatta
     9901 aattataagt atggtattgt gacgcgttgt ggtgcgcatt gtgcgccctt gatgcataaa
     9961 catttaaaga cagaagaaag gggaattgta cgtttgtcta tatcatttca aaatacaata
    10021 gaagatgttg attttgtggt acgtgcaatt caaaagattt caaatgatta ttagtgtaat
    10081 tggttcgggt ggaaaaacaa cgtatatcca cgagttaaaa gataaatatg tttctttaaa
    10141 taagaccgtt ttaatgtgta caacgacgca tatgttgatt gaagaagata cattagtcga
    10201 tcctggttat gatgaaatca tgcaaagaat tgaaacatat ggatattgtc atgcgggaaa
    10261 tcgttgtggt gacttgaaaa ttgaggcgtt ggatgaagaa ttgttaaatc aattaaaaca
    10321 agtggtggat gtgatactga tagaggcgga tggttctaaa catttacctt tgaagtttcc
    10381 tagcgctaat gaaccagtca ttgattcaga tacggatgag gttgtgttga tttcaaatct
    10441 aaatggatta aatcaaccag taaaagatgt ggtacatcgc tacgaacttg cgaatttaga
    10501 tccaagtgaa cttgtaacac ctagaattat gcaagattta atatgtgctt atttaaagaa
    10561 actaaataaa cctgtaaaga ttcatgtgaa tggagctagt gatttgtata cgcgttgtgt
    10621 aaaggcttta ttagaagaaa atatggatgt gaatctcatc caaaaggaat ggtttaatat
    10681 gcagcctaag ctagtaattt taggttgtgg tcatgtcagt cagtatttgg ctaaaatggc
    10741 tagtatatta gaactatata cgattgtgat tgataatcgt aaagaatttg cgaatcggga
    10801 atgttttcca accgcaaatg aaattcattg tatcgattat gatcaaatgg actctgtatt
    10861 accagatgaa gagaatgctt gttatgtgat cgtaacaaga ggacataagg atgatcgtct
    10921 ttgtctagaa aagacgattc ataggcctca tttatatttg ggtatgattg gaagtaaagg
    10981 aaaagtaaaa aagacatttg atgctttgat tgaagaagga tatttgaagg aagaattatc
    11041 gaatgtgcat gctccaattg gtttagatat taaggcacag acgccggctg agatatcgat
    11101 cagtattttg gcggaactta ttgaaataaa aaatacaaag tttagttcaa gtgtttcgaa
    11161 agaattatta gaatccaata tgcatggaac tttatgtatc atcatcgata aaaagggttc
    11221 agcaccaaga ggggttggca gcatgatgtt agtgcataaa gatggagtca ttgatacgat
    11281 tggtggagga aaagttgaat atcaggctat tttagatgcc aaagaatgta aagaagttat
    11341 gatcaaggaa tatgatttat ccaatgcaga aagtgctaca cttggcatga tttgtggtgg
    11401 atataataaa gtcttgttta taccggtata gaggagggat tggatttgaa gaaaagaaga
    11461 agacgagtaa gaaagtcagt gatttttgtg attgttgtat tacttttgat tattgcgttg
    11521 ttgatttcga tgatcgcaaa aaaagttagt gataaagagg taagtgaacc taaacaagaa
    11581 aagacaacag aaacaaaaaa agagaataag aagcaacaaa caaatttacc aaaggtgaaa
    11641 aaaataaatc aaagtggtga cccatatgag aatacagtaa cggtttcgga tttagattca
    11701 atctatattc ttgtgaataa gtattcagga ttaccagcag attatgaacc aagtgattta
    11761 gttcaggtac cttcgagtgg tgaaaatgag aatgtgcgca tgcgaaaaga agcggcagag
    11821 gcatttgaaa agttgattga ggcagcatca aatgaggggt tcattttgaa tgcttgttca
    11881 gcttatcgtt cttatgcata ccaaacagat ttatttaata ggggagctgc aagcaatggc
    11941 gaagagtatg cggatcgtta ttggactcgt ccaggatata gtgagcacca atctggatta
    12001 gcagtggata ttcgtatgga taatgattgt agtgatttag atgccgttcg aaataattcg
    12061 cactatgatt ggttgttaga aaatatgcat atgtatggct tcattttgcg ttatcctgat
    12121 gataaggaag ataaaactaa aattgcgcct gaaagctggc acttgcgtta tgtgggtcaa
    12181 gatttggcaa cgtatttgta taaaaataat ttagcgttag atgaatatta tggagcttag
    12241 gcaatcattg gattgccttt ttttgattta tcaaatggta tattgtatac taaaaacgac
    12301 gtatataaag gattaaacta tgaatataga taaaaagata atagtaggtg taagaaataa
    12361 ggatgcgaat gcgaaaaaag agcttatttc attgacgcga aatcatttga tacaaacgat
    12421 ttacaagcga tatattggtt tagaagaaaa agaaatcaat gatatcttac aggcaacgta
    12481 tacatatgca tttatgcata tggataaatt gagtgatgat aaaaattttt taaaatggat
    12541 tacggtcttg ttgaataaac aattgcgtga gtatatgcag gctaaagagc atgcggatgt
    12601 aactacagct ttgcgtggac ttcctcaata tagacaatcg gatgtggatg attgcttaaa
    12661 tgagatgcca gaaaatcaaa agacagtgac aatcatgcat tgtctaggga aactgaaaga
    12721 gaaggatatc gcgaatcgtt taaaaatgag tgaagaggga gtggctcgtt tattaaagag
    12781 tggtaaacaa aaagtgaaag atgtattgga aaagaagtaa cattataact tttgttgaaa
    12841 tgaattttat taaaatcgat tttaataaaa gaataatact cttttgtaat gaaaatgtat
    12901 tggataagaa gtgacaatga tatttattat tgtccttttt tttgatttcg cataaaatgt
    12961 aggcatatga aaagagacac aaaaagagta ttaatactat cacttaaaat aggtgtgggc
    13021 agtgcaattg cgatcatctt agcttatttt ttcaaattgg ataatccaac aagtgcagga
    13081 acgattgcgt tgttatcttt gttgacaaca aagtggggaa caatgaggct tgttcttaga
    13141 aggatttcca ccttctttgt gacaatttta ttctgtttta ttttctttga attaattcca
    13201 agccattgga tagcttttgg acttgtgatt gcgtgcttag ttgggtacag tgaaaaaatg
    13261 aagtgccaga atacattaag tgtaaatgcg atgattgctg tgcattactt gagttatttg
    13321 gattttagtc ttcactttat gatgaatgag ttttatttga ttttaattgg agccattatt
    13381 gccttcttat tgaacctggt tcatgattat agtggagaag aggagtattt gaattcttgt
    13441 atgatttata tggaggacaa gattcagagc ttgatgtatc aaattgttca ctatattcaa
    13501 agtgaagaaa gaaacacgac aatttggaaa gagttggaag atgtaaaaga acaggctgaa
    13561 aaatatattc atattgcgat ggaatatcag gataatacgt ttacgaattt gccagattat
    13621 tatattcgtt attttgaaat gcgtgcttta cagtgtgaca ttctgcatat gttgcactat
    13681 aagattcgaa agattcgtaa gatgccaaaa gaggcgaatg aattagcgaa ttatattgaa
    13741 tatttaattc cgtttattca tgaaaaaaat gatccgcagc ctcaaattac ttcacttcat
    13801 cagatgttca aaaacaagca gggggaggct cttcctaaaa gtcgaattga atttgaatca
    13861 aaggcaatgc ttctgcatat ttatatggat ttagaagagt ttttatacac aaaaaagaag
    13921 tttattgatc aaacgacaga ggaacagaaa aagttgtatt ggagatgata tacatgaaat
    13981 caatcattat tgtaactgtt gtatctatta tagtttctat tggaatctat aaaggaattc
    14041 gtaaagtatt atataaaaag gatgtcacaa ataaggctag ttttgccaga ttaataaaat
    14101 atattttggt ttttattaat ggaatgattt ttcttggaca atttgaacag acaaaaccta
    14161 ttatgtccta tcttgctgca tcgagtggag tggttgcctt agttttaggt gttgccgcta
    14221 aagatgcctt tggaaatcta tgtagtggtt taatgatttt aacatttaag ccatttgtcg
    14281 agggcgattt gattaaagtc aatcaaggtg aattaattgg ttatgttgaa agtattcgtt
    14341 ttcgccatac gattattcgt acatatgaaa gtaatcgtat tattgtgcca aatgcaacat
    14401 tgaattcggc tacgattgaa aatgcatttt ttcaagatac acgaaaaaat aactatttag
    14461 aaattgaggt ttcttatgga acggatattg atttggcaat tgatatttta aagagtttag
    14521 ttgaaaaaac aatgattgaa tatgaaattc aaagtgagga tccaattgaa gtaaaagtaa
    14581 tcaatttttc tacaacaggc attgatttgc gtgttgttgt tccaagtaaa acgagtttag
    14641 agggttttga tatgctcgca aaaattcgtt atgaacttat aaaagagtat gaaaaagcga
    14701 atattcattt tggaaattat tcagtagagg taagtcaatc atgataaaag tatatacaga
    14761 agtcttggga tttaataatt taatgtatcg ctacatgagt ttacctaact cattatcttt
    14821 ggcggattta gcttattata cattggcatc ttacttatct gaagaatatg ctgattttgt
    14881 gattgagtcg aatggtattc cttttgcgtg tccaaattgt gaggatgaac aatttaataa
    14941 gttaccttgt gcttcatcga tccagcctga tttcaaagag ggagatactt ttaagatggt
    15001 ttatgatcta gaagagcctt ttgagattga aattcaagtt gaaaaagtag atgatataga
    15061 agaagatgcg aatgtggtca atggtcgtgg ctttgcgata tgggatggtt ttaaagagtt
    15121 tcaggattat tattatcaat ccatggaatt atttaaagaa tatgtgaaag cgaatgatat
    15181 gaatttatcg gattttcctg tggatgaaga tcttgatatt attgaattaa acaaggtatt
    15241 ggaagataat tttgaagaat taaaagatgt gtatgagggt gagtagatga aagtatatga
    15301 gatgagtttt cgtgatatgg atcaaaaaat ggtagagatt catggcgtga aaatggttaa
    15361 gcttttagag aagatgggat taaagttaga taatctatat ggtgcattaa tgtatggtta
    15421 tatcgatcat aatgcgggct ttatttttga aatcgtagct ttggaaacaa agaaacgtaa
    15481 tattgaatat cgtattgtgc cgattggtgt atcttgtaag attcctcgtt ttgatgtgca
    15541 ggaaatggat attcaaatct tggataatgt gaatgtggaa ttgttccaag ataagattga
    15601 tatggttgaa aaggctacag aagtttctaa agaattagaa gagcttcgtc tatataaaga
    15661 gttggattca tccagacatt tagagtatcc agatgatatc atggtatatt ttttagaaga
    15721 aggaaaagat gtagaggcat gctgggttcg tcttgaagga atacaagatg gaaagatgta
    15781 tggaagtgta ttaacggctt tgcaccagga ttttggtgtg aaagaaaggg atacaattta
    15841 ttttggcatg acagaaatgg aagatcacaa gttggcgtgt gtgtgggtga aagaagatga
    15901 ataaagagga ttcattatat tcaatttatg tacaaatatt gaaagaagaa ttagtgatgg
    15961 ctatgggttg tacagagcct atcgcgatta gttatgcgtg tgctaaggca acgcaagtgc
    16021 tagatcattt accagatcgt attgttgtta aggcaagcgg aagtattatt aaaaatgtga
    16081 agagtgttat cgtgcctcat acaaatggtt taaaagggat tgaagttgca gcagctgcag
    16141 gagctttata tggggatgcc gacgcaaaac ttgaagtact ttctagtgcg acgcgtgagc
    16201 aaattgaaga attaccagaa tatgttcaga acacaaatat tacagtgcag cacattgaac
    16261 aaggtcatgt atttgattta gaaattaatg tttattatga acaagaacat gcaagtgttc
    16321 gtattgtaga tacgcatacg aatattgtgc agattgaaaa agactggaaa gttgtctttg
    16381 aagataagac gacaagctta gaacataagg cggatcatag tgctttgatc atgaaacaaa
    16441 tttgggattt tgccaaaact gtggatattg aggatgtaaa agaaatattg gatcgtcaaa
    16501 ttgcttgtaa catggctatc gcaaatgagg gaattcataa ttcgtatggt gcaaatattg
    16561 gtcatgtgat cttgaatatg gatttggatt gtgtaaagac aagggccaaa gcttatgcgg
    16621 cggctgggtc ggatgcaaga atgaatggtt gtgaactacc agtagtcatt aattcaggaa
    16681 gtggaaatca agggattact tgctgtgtgc cggttgttgt atatgccaaa gaattgaatt
    16741 ctacgcaaga acaattgtat cgtgctttag ttttatcgaa tttgactgct atttatatta
    16801 aaacaggtat tggtacttta tcagcattct gtggggctgt tagtgctgga gctgcagcag
    16861 gagctggtat tgcctactta catgatggaa catatgaaga aattcaacat accattgtga
    16921 atgcgttagc tattctttcg ggaaccattt gtgatggggc aaaggctagt tgtgcagcta
    16981 agattgcttc tagtatagat gccggtatca tgggttatta tatgtataaa aataagcaac
    17041 aattctatgc gggagatgga attgttgctc atagtgtgga tgaaacaatt caaaatattg
    17101 gaactctagg ttctcaagga atgcttcaaa caaatgataa aattattgag atgatgattt
    17161 cttgcgatta aaagtcgata agcgaatgat tagttgaggt tcatattcca tatggacgat
    17221 acgttttcgt tcgctttttg tttgttctag aagaatgtct acagcttcct tgcctttttc
    17281 acaagcacca tgatcgacgg ttgtaagttg aatttgtggg aaatctgttt ctggaatatt
    17341 gtcaaatcca gctacacttg ttttgatgtt gtgatctttt aaacaggcta agatacctaa
    17401 agctgtcatg tcattatttc ctatgtaggc atcgtattta tcgcctaaaa tttgttttgt
    17461 caaacgatac ccgttttcat attccctttt ttcaattggg tacatgtcaa attgtttaga
    17521 agaactaaag aatacttgtg tgttggatat tacacttttt acaccttcat atctttcgtt
    17581 tctacctatt tctgttgtat ctaatggact tgaaatatac gcaattttct tatgtcctag
    17641 ttgttttaaa tgattaccta atagatttcc acatttaaaa ctgttgagtt caattgaatc
    17701 acaatcgaga ttttttggct tatctccaat aatgatgcat ggaatttgtt tggaaagtgc
    17761 attccattca tcaacaaatt ttgggggata tagaagaatg attccacatg gttttaatag
    17821 ttttaagctt gaaagtgcat gtttttcgag ctcatcttta cgaagtgtgg aaattgtaag
    17881 gatttgtgtt ttggtcttgt ttgcttgttg aatgatgctt gaaacgacca tagaataata
    17941 tgaatttgat aaattcgaac aaataacgac aatcgtattc ttagtatttg aatatctttt
    18001 ttttacttct ggctttttat atcccatttg tttggctgct tttttgacaa gtttaacagt
    18061 ttcttctttg aatgatacat ttttattatc acttaaaatc atagatacag tggattgtga
    18121 tatatttata gctttggcta tatcgcgtgt agttactttg tttctttttc ccataatgaa
    18181 cctcttttga tttattttga ttaatttatc gattactaat caaattattg atcaaagcct
    18241 atttttatct tacaccattt tatttgattg tctagctatt aatcaataat ttgattaata
    18301 aaatcagttg gttttacccg tttgaaagga aataaaaata ggcgtatcgt tactttgaaa
    18361 gaggtgtgaa ctatgtatga ttgtataata attggagcgg gtatcagtgg atgctcttta
    18421 gcttttgaat tgagtaaata tagtggaaaa gtattgtggt tagaaaaaaa taatgatgtg
    18481 gcagatgaaa cgaccaaggc caatagtgca attattcatg cgggctatga tccagagcca
    18541 aatactttga tggcaaaata taatatttgg ggtaataaga ttattccttc tttatgtaaa
    18601 gatttagatg taccttgtaa aaatattggt tctttagttg ttggatttag tgaagaggat
    18661 gataagacaa ttgaagtgtt atataagcgt ggtattgaaa atggtgttga aaatcagcgt
    18721 attattaagc agcctgagtt atcaaaaatg gaaccaaatt taaatccagc ttgtacaagc
    18781 gctttatatg caccaagtgc atgtattatc aatccttggc agtttgcgat cgcattaagt
    18841 gaagtggcaa ttcaaaatgg tgtagagctt cgtttgaatg aagaggttac gagtataaaa
    18901 aaagatggag atatctttat cgtgaatgat acatacaagg ctaagttagt tgtaaattgt
    18961 gcgggtgtta tgtgtgatca tgtgactgga cttatcaaga agccaaatta taagattacg
    19021 ccgaataagg gtgagtatta tttattggat aaatcacaag gaaacttggt tcatcatgtt
    19081 attttccaat gtcctacaaa gattggtaag ggtgtgttgg tagctcctac tgtagatgga
    19141 aacttgattg taggtcctac aagtacaaat acagatgtgg atgattattc aacaagcgct
    19201 gcaggtttaa aaacaattcg tgaaaatgcg gtgaaatctg taccgaatat gaaacttcaa
    19261 gaaaatattc gtacttttgc aggtttgcgt gcaagaagtg ataaaaaaga ttttattgtt
    19321 ggtgaagatg aggatgtgca taattacttc tcgattgcag gtatggcaag tccaggttta
    19381 agtagtgcaa gtgcaatcgc attagatgtt gtgagaatga tgcaggaaaa gaattatttt
    19441 ttaaatttaa agaaggatta tgtttctaca agaaaagttg ttcgatttaa agaaatgtca
    19501 catgaacaaa gaaaagaata tattgaaaaa catcctgagt atggtcgtat tatttgtcgt
    19561 tgtgaaacga tcagcgaggg agaaattatt gatgcgattc gtcgtagtcc aaaaccagtg
    19621 agtttagatg gaatcaaacg tcgatgtaat gcaggtatgg gaagatgtca gtctggtttc
    19681 tgtggaccta gagtacaaga gattcttgcc aatgagttgg gcgttagtca actagatatc
    19741 atgttagata agaatggttc ttatatttta tgtggaagaa caaaggagaa tgacgatgaa
    19801 taataagtat gatgtagtag tcattggtgg tggtcctgca ggtcttgcca gtgcgatggc
    19861 agccaaacaa aatggcgcaa aatctgtttt gattattgaa cgagataaag agttgggtgg
    19921 tattttaaat cagtgtattc ataatggttt tggtttacac cactttaaag aagaattaac
    19981 aggcccagaa tatgccggta aatatattga tgaagtcaat aagacagata ttgatatctt
    20041 attggataca atggttattg agattacgcc agataaaatg gtgtattgtt caagtagcat
    20101 tcatggatat ttaatgattc aggctaaatc aataatttta aatatgggtt gtcgtgaaag
    20161 aacgcgtgga gccattgcga ttccaggcac aagagcttct ggtgtattta cggcaggtgc
    20221 ggcccagcga tatgtaaata ttgaagggta tatgcctggt aaaagagttg tgattttggg
    20281 tagtggtgac attggtttga ttatggctcg aagaatgact ttagaaggcg caaaagtttt
    20341 ggcagttgtt gaagttatgc cttattcgaa tggcttgaat cgtaatattg ttcagtgcct
    20401 acatgattat gatatcccat tgtatttaag ccatacaatt acggatattg tgggtaagtc
    20461 tagagtagaa aaagttgttg tttctaaggt ggatgaaaat agaaatccaa ttgaaggaac
    20521 cgagatggaa tttgattgtg actgcgtttt attgtcagtt ggattgattc cagaaaatga
    20581 gttgtcaaat gatttagggg ttgaaatgga ttctagaaca catggaccta ttgtttatga
    20641 aaatatggag acttctatgg aaggggtatt cgcatcaggc aacgttgtgc atgtgcatga
    20701 tttagtggat tttgtaagtt tggaaagtga aaaagctgga atgggtgctg gtcgatatgt
    20761 ttcaaatggc gaagtatcta agaattgtgt cgttaagacg ataaatggag ataatatttc
    20821 gtatgtggtt ccgcaatata ttcgtaagga taatgtagaa aagagtgttg aactatcttt
    20881 ccgtgtaaga agacctgcac aacgggtaaa tattgttgtg aaggatgggg atactgtaat
    20941 tgcgaagttt aaaaaggaac atgtgatacc aagtgaaatg gagaagatat ctattcctaa
    21001 agtgttgctt gaaaaggcag ataaagaatt ggttgtatct gtaaaggagg atgtgcaatg
    21061 agagagttaa tttgtataac ttgtcctaag ggttgtcact taaaggtgga tgaagaaaat
    21121 gattataaag taactggaaa tggatgtcct agaggtgcaa tctacggaaa aaaagagtgt
    21181 acacatccaa ctcgtgtggt gacaagtaca gttaaaattg taggtgctga aattgagaga
    21241 ttaagtgtga agacgaatgg ggatattcca aaagaaaaaa tctttgatgt gatgaaagag
    21301 ttgaatcatg tagttgtgaa ggcaccagta catcgtgaag atgtaattgt ctctgatatt
    21361 tgtggtacgg gtgttgattt agttgcgacg aaagatttat aatatgactg ttaaaagtgg
    21421 ggagctttta ggctgagagt aggctagtcc tagacccaat aacctgattt ggataatgcc
    21481 aacgtaggga atatatcttt ttgataggga gctgcagatt ggtttttctg cagctttttt
    21541 ttggtggtga aaaaatgaat tattttattt ttgatgtgga tggaacaata ctagattcaa
    21601 tggaagtatg gaatactttg gcaagtcgat atgttcaaag tttgggtatg attccccaaa
    21661 ataatcttga tgaaattgtt tcaaatatga gtttagaaca gagtgcaaca tatttgaaga
    21721 agcattatgg aatcaataaa tcagaagaac gaatcatttc agaagtattg aatcttatat
    21781 cggattttta taagtatgaa gtgaagctta tgcctgggtt taaagatttt atttcacatt
    21841 atgattcagt caatgtgatt ggtacaagtt gtgatgaaaa gctagtaaaa attgcgttga
    21901 atagactggg tgtgttaaat aattttgaag atattataac ttgttcaaag gtgaataagc
    21961 caaaggatga tccgaatttt tatttagctt gtgcgcaagt tttaaaacaa agaccagaag
    22021 atatagtggt gtttgaggat gcagattatt gtatagatgt ggcaagaaaa attggattta
    22081 aagtgataaa aataaaagat tggagagatt taaataaaaa gtgcattaac aattgcggga
    22141 agtgattcga gtggaggagc aggcatttag gcagatatca aaacaatgtg tgctaatcat
    22201 gtatttgcga caagtgcaat tacggcatta acagcgcaaa acacattggg agctacaggt
    22261 attgtggatg taacacctga atttttaggt gaacaactag atgcagtatt tacagatatt
    22321 tatccggatg ctgtaaagat tggaatggta tcaagttcta gtttgattga aatgattgca
    22381 aagaaactta aggaatataa ggcagaaaat attgtggtcg atcttgtaat ggttgcaaca
    22441 agtggtgcaa gattgattag tgaagacgcg attgaaacat taaaaaaaga attgttgcct
    22501 ttagctactt tgattactcc aaatattcca gaagcagaag tactttcaga aatggatatt
    22561 atggatgagg aaagtatggt tgaagcggca aaaaaatcag tgaaactttt ggatgcgcta
    22621 ctctagttaa gggcggtcat caaatcaatg atgcgaacga tttactatat agaaacgatt
    22681 catttacttg gtttaaaggg aaacgtatca ataattcaaa tacacatggt acgggttgca
    22741 ctttgtcgag cgcaatcgca tcaaatcttg cgaaaggatt tgatttggat gattcggtaa
    22801 aacgtgtaaa agattatatt tcaggtgcgt taagtgcaat gcttgattta ggtaaaggct
    22861 caggtccgat ggatcattcg tttgcgataa ataatgaata tacaaaggaa ccattctagt
    22921 gttggtgtct agaatggttc ctttttgttt attattattt tttaggagag agatatgaat
    22981 attggtttat tttctatatt cattgcctta tctttaaggc acttatagaa tatcattcta
    23041 tgttgtcata aatttagtat aactttggta aatcttgtga aatctgattg aattatgtga
    23101 aattgtcaaa atatgcatct tttttggaaa agcctcaatt tatttcgtat tagatatagg
    23161 ggtgagtaaa tcacatgaat aaaaccaaag acaaaattat gaaagaattt ctggaaaaca
    23221 atgcctattt tgtcgacttc tttaatgcat acttctttga tggagaaaga gttcttaaac
    23281 ctgaaaactg tatggaactt gattcagaga tgaatgattc gaatatggag ttagaaaagc
//
LOCUS       DBMDWL010000011        22016 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_863_length_22016_cov_3.048095, whole genome shotgun sequence.
ACCESSION   DBMDWL010000011 DBMDWL010000000
VERSION     DBMDWL010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 22016)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22016)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22016
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_22016_cov_3.048095"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="ACZPDN_01465"
     CDS             <1..922
                     /locus_tag="ACZPDN_01465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1297324.1"
                     /translation="SKQEAKLSGYSALAQIYISMHYINVKTQKYHIVKTEPEILKYLQ
                     VDKINDVEDCFSDHIYKIHKEFCQQDYVDREIAFMDLETLDERLQNKKSIDSVFYGKI
                     SGWCRGRYIPVDYDEDGHLLHVLYCVECIDGQKRREDKLLYLAQTDTMTGVSNRRSGE
                     KMIERILNNKISGMMCLVDCDKFKLINDTYGHMAGDEVIIAIAHTLQKSCRDKDIVMR
                     LGGDEFALYIPGVTNRKCANSFFKRLFENLKQIHVESIDNHPVVLSLGACFFDGKEDL
                     SFDELYCRADSAMYESKKIDGYSATIFRKK"
     gene            1035..1811
                     /locus_tag="ACZPDN_01470"
     CDS             1035..1811
                     /locus_tag="ACZPDN_01470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1297325.1"
                     /translation="MQKYVEIKRDDLTLRGMLHIPNDVSQKVPMVILLHGFCDDRNEI
                     NFVHNELSQRLCDAGIASVRFDMNGSGESDGRFEDMTVSSEILDAQAMLRYVRSLDFV
                     DTKKIALHGCSLGGCVASMVAGKCKDQIQALSLWCPAPDLVYNLKEHKTLCNQDVSNI
                     EKDGCADVEGLKLSLKFYQDACTLDPYKEASLFDKNVCTIHGDQDITASCECSYKYKE
                     IFKERAKCIIVKGAEHRFKSFAFREARMQGALDFLKEELL"
     gene            1832..3229
                     /gene="nhaC"
                     /locus_tag="ACZPDN_01475"
     CDS             1832..3229
                     /gene="nhaC"
                     /locus_tag="ACZPDN_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HIR1297326.1"
                     /translation="MNHERKEPTTLFAIVSFLVIITVLIIMINMGCNTTLSVFSGVIV
                     SVITTRLLNIPWNEVEQNIIKTAAECMPTFLIVIMVGMLVGIWMAGGTVPTLMYYGMQ
                     VVSPKILVPLAFVLCALTSEFTGTSFGSVATMGLAMVGIASTTSIPVPLVVGAVVSGA
                     WLGDKMSPLSDTTNLASGVSKVPLYDHIHSMQYTTLPAAVVALVLFTIAGLYYSGGSM
                     DLAQQKLIMDTLSQHFTINILLILPAILVILISVFKLPSLLGMGLAVVVSAIFAMVFQ
                     GVNFVDLMNYAFNGFSIETGVSIVDPMLNRGGVLSMSELLVTFMVASVMGAVITSSGI
                     LDVLAKNVLLKFIKNRTVLVFVTLVYCYIVNFLTAGGQTVSIIVTEQTFESTYEDMNI
                     SRKVLSRTLEDSGTLSAPIVPWGVATIYVMGVLGCSTAYIPYAWFIFIVPVFSMICAI
                     TGIGVWDKEEKPVRK"
     regulatory      3278..3384
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3486..4826
                     /gene="thiC"
                     /locus_tag="ACZPDN_01480"
     CDS             3486..4826
                     /gene="thiC"
                     /locus_tag="ACZPDN_01480"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR1297327.1"
                     /translation="MNTNESTVSTGATYTTQMDAARKGIITPEMKIVAQKEKMTEEEL
                     RALVACGKVVIPANKNHKSLDPEGVGSMLRTKINVNLGVSRDCKDYDVEMKKVMAAVD
                     LGAESIMDLSSHGNTQPFRQKLTSECPAMIGTVPVYDSVIHYQRDLNTLTAKDFLDVV
                     ELHAKDGVDFETLHCGITRKTIDQIKKHKRKMNIVSRGGSLVFAWMSMTGEENPFYEY
                     YDEILDICEKYDVTISLGDACRPGCLADATDVCQIEELVRLGELTKRAWDHNVQVIVE
                     GPGHVPLNQVAANMQVQQQICMGAPFYVLGPLVTDIAPGYDHITAAIGGAVAAMSGAA
                     FLCYVTPAEHLALPNVEDTKQGIIASKIAAHAADIAKGVPGARDIDDKMADARKNLDW
                     DAQFECALDPETAKAIRQSRMPEEDHSDTCSMCGKFCAVRSMNKALSGENIDIL"
     gene            5074..5646
                     /locus_tag="ACZPDN_01485"
     CDS             5074..5646
                     /locus_tag="ACZPDN_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1297328.1"
                     /translation="MIDGHDQADIYILKDGIVKTSIILSDGREFNIMYLKGFDVLSLL
                     KDEIKKVTSASFHIRIESDTAVFYRVNRKLFSKFLSENKELRDYMDAYYRKKLSEAIY
                     RQQLMTMNGKNGAVCAFIYYLVGLFGREVKKGIFIDLQVTNDDIAGFCGVSTRNSVNR
                     ILRGLRQQGVITMVYHKILVLDVDYLKRYV"
     gene            complement(5660..7285)
                     /locus_tag="ACZPDN_01490"
     CDS             complement(5660..7285)
                     /locus_tag="ACZPDN_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR1297329.1"
                     /translation="MQLSFKSKERSMKRKLFVYMFFLVTTISITLLMGLFLFGRLGTT
                     EQDFHKDLTTQSEYFTKNMENYWDDLASMNIALADNMEAILETTLANQGLTFQQLQDN
                     PNAIYSLENDMIQPLGQYLERSNCSGAYVQLDTTINSTLDNAQTQRSGVYLQKTTMSY
                     SKEDLILYRGIANIGKKHGIMPHRKWRQEFDITLVPDYEELKKGNFDYSLSNIIQLPY
                     TGERIALLRVPLVGKDGTFYGLCGFEISQSWFKFAHPQPSTLNHLTCLLVPDSDSIIT
                     NEGFSTGSQNGFYYMPNGDLSIKPIGNNLLRIISKNRNYIGIKNTVKISKNQTYSTYV
                     MILDSDYNRAIAKSNIELFFFTIVLLLFVFLGCSFFSQRYLSPILKGLEQLRKDNKNS
                     MRYDVQEIDDLLDFLSSKDYEVENKMLTLELEMQENRKKLEQIENEHEAIQKEYDRAK
                     KEVERLTTKRIDDIDEDEYKRFLECLATLTRKEKEILNLYVKGYSSKDILETLNITDN
                     TLKYHNRNIYSKLGVKSRKQLLMYMTLLNNKRS"
     gene            complement(7295..8623)
                     /locus_tag="ACZPDN_01495"
     CDS             complement(7295..8623)
                     /locus_tag="ACZPDN_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024808.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1297330.1"
                     /translation="MKKVIKYLLVFGLSLCNLYGCSSKSLLDKDNPVTLTMWHVYGEQ
                     ANSPMNQLIAEFNETEGKDKGIIINVTKITNASKIGSELEESLSKEPGADPMPDLYTG
                     HIHNAKQVGLDKSVVWNDVFTEKEISNFVDEFLDAGMLEDKLVDLPLSKSTIVLFMNG
                     TQFEKFSNDTAVSLNDFDTWNQTFDVAEKYYEWSNGKPLLCFDYLLNSIESYAIALGS
                     KHMYNENGWYNTDDKEFQEAFRTFLEPLIKGHISASDFYSSTELTTGETLIGVSSSAA
                     YLYLNDIVTYPDNTTEPLNAIALPYPKADKDHALMTQAGVGLCAYKTTEQKQEAASVF
                     AHWLVEPKRNMDFVIQAGYMPVTKKAFKNLKNEKFEDEETQSLYNTLTEMKSDYTVIS
                     REVPNAISRKAYAFYDQVRSHQQEWQARANAGEDIDEIVDEAMNILKSLS"
     gene            8801..9421
                     /locus_tag="ACZPDN_01500"
     CDS             8801..9421
                     /locus_tag="ACZPDN_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025826.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HIR1297331.1"
                     /translation="MGLFSKKECDVCKKKMALMEGYKCADGTICNDCKKKMSPLFTDY
                     KNTTITQIKGQLTYREENAQRVRVFVAAETFGEYPKVMVDRKHNWFVVTKATKLSDEN
                     PDVFDLSDIQNVLVEIEENKRELHFTNKKGKTCSYKPARYEFTYQFKVNIQMNHPFIK
                     MISLKITKEDVKIISEMQDKEVMVQSNEKYQESLQVANALKDALES"
     assembly_gap    9612..9621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9622..10200
                     /locus_tag="ACZPDN_01505"
     CDS             <9622..10200
                     /locus_tag="ACZPDN_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297332.1"
                     /translation="TEVGTKIWQDLMEPLKKTRDKNQKMQSFHPIYIAPSLGLIAVIE
                     ARGGLFNTKTYACVYRLENLQIYKTENMPARTSGSDKDKKCVHLGFVHTKGLNDVYIP
                     FDDETRCHQCVDYLNKLFGLDDSFRSGIKKSVTQFKATKSMWDLAKAKKNGESLEEKA
                     KATVDAFGATIIGDRTQFKDAADQALAGYDLD"
     gene            10187..11473
                     /locus_tag="ACZPDN_01510"
     CDS             10187..11473
                     /locus_tag="ACZPDN_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297333.1"
                     /translation="MTWIKLCSVLFLSLNLMFTGCKSQPNEEVYISDFYNIHEEYKDA
                     WGNVGKYDISLPYIHCDSKNAKKLNQTIKNEYKDLVDSLDEAKEEGYTLPYTKVSYDV
                     YTHSNLLSLVIKEEVETEYPRYKVYHFDSKTKKRVSNKKLYKKYKISQKDMKVWAAQA
                     FDQEYANEYYDNVKELRAQTIGLLPSNLDVFYHKGKLNVLIPIATNFGSGTKMVLYRP
                     SRKKSYKIEDSSTFDQMTMTLKNNKLSLYYSGTDKTYKVHGCFNEYKQVHVYEIGPGV
                     TYGFALTTNGLVEVIAIEDGLPIERVCSQGPLYGLDKVKSLRSGQALTENDQLIDLFD
                     SLQNQPFKNLEGTYTNESVTLQIDENGLYAMQDQENSHSGLLFSVGMEEDGFVYGYDD
                     YNQQVLSYCKVMPLYEKLEVKNLFDHDKLELEYKYE"
     gene            11466..12098
                     /locus_tag="ACZPDN_01515"
     CDS             11466..12098
                     /locus_tag="ACZPDN_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297334.1"
                     /translation="MNKKVLVSAFALSMVFGLTGCSSEKPEEKKEDTKVVEKKEEKKE
                     EKKEESTDAVLVREISSKTYTVTIPQLTSFYTPSRDGLEVKTEVNEQQISYTLEDEFG
                     DFKMAIYVNPISAESAEAYANRINEGFKDDESKQYKPSTVGDFSGFRKVTTSENPSFK
                     CKDNLLLDFESVDGSAVVMSVTVEQFNDSDTADMEVAMKAILGNMKVEVK"
     gene            12101..12748
                     /locus_tag="ACZPDN_01520"
     CDS             12101..12748
                     /locus_tag="ACZPDN_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297335.1"
                     /translation="MNAKFVKSLLVIGMGLSLAACSSNDPEPKKEDTKVVEAKKEEKK
                     EEKKEEKTTDFVVSKNYNKKYEIIITMPQDFTTIYTPANGVEVTGEIVNDSFIRYSFE
                     NKEADTKKIAAIEANADATADEVAETFNKGEEDESKLYKAYQVGNYTGVNKISDYSYD
                     DYVTYNMYFNLDRDGFKGTIKVVVEKLKAEADDSDTEAIITAILDNVKVEKKKCE"
     gene            12741..13352
                     /locus_tag="ACZPDN_01525"
     CDS             12741..13352
                     /locus_tag="ACZPDN_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297336.1"
                     /translation="MSKPMNHKLAKILLALGIGLSLVSCAKKPEEPRSSQVKKEEKVK
                     IGEMTGVAQMNGYFIKMTMPDLLSVYTPKQEGLDISGKLEGGRILKYDLHSDDRTYIT
                     RISIDCDYRKAAKETADVLNAGFEDEADQYKAFTIGKYSGYKKTTIYDSSDKTYSKML
                     IDYPVGEDNVVLQIYVEQEKANDLDEVTRAILDNIVVEICMEE"
     gene            13670..14035
                     /locus_tag="ACZPDN_01530"
     CDS             13670..14035
                     /locus_tag="ACZPDN_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297337.1"
                     /translation="MEESNVLGYSLYAKDRTFTIHFSIGTDGWDSAEAASISHNKVCK
                     DESKMYEPCTVGEYSGYKYVVSSDKSIEYHYVLDYLVDGQDVVLTIREELRNDDDTSL
                     LEPLLLDVVHNLKIELIGE"
     gene            14037..15188
                     /locus_tag="ACZPDN_01535"
     CDS             14037..15188
                     /locus_tag="ACZPDN_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297338.1"
                     /translation="MNGNKLLTGFLILGMGLSLVACSNEKPETKKEEAKVAEKKKEEK
                     QIVTQDRTAKLSIVEDLSPYFTSSREGVGKMEGMLKDNGSIVYKCLNEENKYELNAEI
                     KLLSSSSAEKSADLQNSMYGENDEDKKYKPCTFEGFDAYSHTINLGTVENTRLHYYVD
                     YPVEDINKVVSIEINQKNDKEKDTSDLEPFAKAIIKNMKLEKVKEEKKEEVKQDVKEE
                     KVDENSKEIVGESSRAKLTLPRDLTSFYTPNEKYDTIKGEQDKGNIGYTLSRKDPDRS
                     IYVNICTDSSDTLEEAIVFHNEHLSKGEEPFTEYTLGEYSGYRSMSYHNYWNSANDTT
                     YRYKLNYPVGDKNVILSCSVTLRDNSDDTTGLEDILLAALQQLKVEIKQ"
     gene            15203..15817
                     /locus_tag="ACZPDN_01540"
     CDS             15203..15817
                     /locus_tag="ACZPDN_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297339.1"
                     /translation="MKNNKLLTSLFVLGMGLSLVACGASDGGTTTKTDGTKTVEKKEE
                     QKFADRKEYETEGLACNFKLPNLDSFYLVKGLEKPVYNTELSLGNTKYEVYDDYLPVL
                     LVYVGDDAFAEWSDGYTIEELATKDLNGEAEYTETKVGGYRALVADLSDEDSLKKEYY
                     IEHPDLPNGIVCIEINEYDDKDISDELAEAIIKEFKVVELLDVE"
     gene            15827..16303
                     /locus_tag="ACZPDN_01545"
     CDS             15827..16303
                     /locus_tag="ACZPDN_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297340.1"
                     /translation="MSNPNDGYAKRKEMSVQGLACTFTLSNMDAYFLVEDEKVSYKSK
                     VVASGMMYRIFGQNMDILVFVDTIPYIRNNAMSYDEVVRVEFENLDYKDLEILAYKTC
                     IYEEEDGNYIKRTYLVDYPDISSGVLRIEILSKSNEFDSLVRAIVKEIQVKTIEEW"
     gene            16313..17659
                     /locus_tag="ACZPDN_01550"
     CDS             16313..17659
                     /locus_tag="ACZPDN_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024813.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1297341.1"
                     /translation="MGLIKAGAGAVGSVFGDQWKEYFYCDALEQDVLVRKGSKRTGKK
                     SSNKGSDNIISNGSVIAVADGQCMLIVDQGRVVELCATPGEFVYDSSSEPSIFAGSLG
                     QSIKDSFTTMAKRFTFGGDTAKDQRVYYVNTKEMGEVLYGTATPIPFRVVVNEELGFK
                     LSVKLRCNGNFTYRVADPILFYTNVCGNVAEDFESETIEGKLKGELMTALQPALAKLS
                     ADRVMYYEIPAHTMDVAAALNDELSDIWRAKRGLEVVSFNINSLSIPEDQEKKITEWE
                     ENAMTMNQTVAASRLVGAQATAMKDAANNASGAMTGFMGMGMAMNQGGINAQNLYAAG
                     AASQPAPDPTTIPTPATTPEVKADGWTCPTCGHLASGKFCPECGTPKPSVEGWACPTC
                     GTVNKGKFCTECGAKKPAHEPLYRCDKCGWQPEDPHHPPKFCPECGDPFDDNDIEK"
     gene            17672..18757
                     /locus_tag="ACZPDN_01555"
     CDS             17672..18757
                     /locus_tag="ACZPDN_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR1297342.1"
                     /translation="MSTLQEFKCPCCNGAISFDTKLQKMKCPYCDTEFSLETLQQYDS
                     VKIEEEDQFEWDVKPGQEWAENETDGLLTYVCNSCGGEIVADENMAAASCPFCGSPIV
                     VKEKLGGILKPDLVIPFKLDKKAAKEGLKQHYKGKTLLPKEFKSENHIEEIKGVYVPF
                     WLFDADADGEVRYNATKVRMWSDSEYDYTETKYYRLYRKGHVRFENIPVDGSSKMPDD
                     MMESIEPYDLKDAVDFQTAYLAGFLADKYDVDADSSIDRANTRVKKSTEDAFMTNCGK
                     YDTVTCEDSAVQIQNGTAKYALYPVWILNTKWRGENYSFAMNGQTGKFVGNLPENKSL
                     VYLYYVVVTLLGSVLTYIVILILSWGK"
     gene            18768..20012
                     /locus_tag="ACZPDN_01560"
     CDS             18768..20012
                     /locus_tag="ACZPDN_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016424.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR1297343.1"
                     /translation="MKKLSILCSSLLLALMMCFPVFASEDLRYVEDNAGLLTVSEQQE
                     LNDTLTEISERQGVDVAVITADDMGDQSIEDYAKAKYDERGYGNDGALLVVNMEVRKW
                     WMSTYGKGTTAISSEDISTIGSEFAPYLSSKEYADAFETYARLCDDYITRAPNLSEEY
                     AQALSGLTSLQDGLRFVNDDARLLTEDERKELNDTLTQIGKRQGLDVAILTTKDTGGK
                     ALLDYAMTMYGALEYGQGKNKDGILLVVNMETRKFWMATHGYGITAFTDAGISYISEK
                     LGPSFKKEKYMKAFTTFGSLCEKFVEKAHNGKPYDVGNMPFKWLPWYCVPISLLVGFV
                     CAVFVVSKKREQLHSVAFEDKAHNYMKKNTLKMKESHDYFLYHTITRNRIKSDDDDSS
                     SGGSSTYSSSSGDTYGGGGGSF"
     gene            20080..>22016
                     /locus_tag="ACZPDN_01565"
     CDS             20080..>22016
                     /locus_tag="ACZPDN_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297344.1"
                     /translation="MMKQCMSVGLALSMLLSLTPITAIHADETQELDVQDTEERKEDL
                     ELVPSVSVAGLKAVATEKESITITFKNVGQQVNNDNENNSFWGEIYFTDSAGKPIPNL
                     DFELHCDDVTTQFVKSDANGKYTFKYVYSKPMYTHRNPVRVYAMVRENDKYVHSYGEQ
                     YYHNPIKWFHFITHEDEKYKTATSITYPFNVTGGEIVEEIEVLKTYEDGTIVFDAWYD
                     DEARTIKHDFTKPIISNVDIYAKWKKIEKPVVPPEPEVERVKITYSPGEGKGPNAVMK
                     IPKSDEDYKVRDFEYSPIGKTCSPPSDKYYFKEWLVKYADGTETRVTPGTKIKITQDT
                     AFIAQWARLYEVKVADTITNGTVVLMNNDREKGQVVTAKEGETILATLTADKGYYNKN
                     IIIQQANGSYLPWKRNEFNPYLVTFKVENHDITFNANFASLTADPEKPIIKARNLKYN
                     GKEQEVEVFGYDSVMMKIEGNKAKDAGTYTVKVTSKTGKWDDGSTDAVTTKWKIETVQ
                     RPAPTGLKGFDTSYPGANDGRITGVTDEMEYIEDTDTKFYPCTGTEITGLKPGLYNVK
                     YKDDKNQTLNISAQIEIKEGTGTVPTHIHTYGKWTSDTTSHWHECTDASCSSIKDKAV
                     HVYDNDADTTCNICGYVRTITP"
BASE COUNT         7304 a         2956 c         4896 g         6850 t
ORIGIN      
        1 gtccaaacaa gaggctaaat taagtggata ttcggcattg gcccaaattt atatttcaat
       61 gcactatatt aatgtaaaga cgcaaaaata tcatattgtg aaaacggaac cggaaatcct
      121 taaatattta caagtcgaca aaataaatga tgtagaggat tgttttagtg atcatattta
      181 taagattcat aaagagtttt gtcaacaaga ttatgtggat cgagaaattg cgtttatgga
      241 tttagaaaca ttggatgaaa gacttcagaa taagaaatca atcgatagtg ttttctatgg
      301 taaaatttct ggttggtgtc gtggacgtta tattccggtt gattatgatg aggatggtca
      361 tttattacat gttttatatt gtgtggaatg tattgatgga caaaagagaa gagaagataa
      421 attattatat ttagctcaaa ccgatacgat gacgggtgtt tcgaatcgtc gaagtggaga
      481 aaagatgatt gagcgtattt tgaataataa aattagtgga atgatgtgtc tagtggattg
      541 tgataaattt aaattgatta atgatacata tgggcatatg gcgggggatg aggttattat
      601 cgcgattgcg catacgcttc aaaaatcatg tcgtgataaa gatatagtaa tgcgtttggg
      661 tggagatgag tttgcgttat atattccggg tgttacaaat cgtaagtgtg caaattcttt
      721 ctttaaacgt ttatttgaaa atttgaaaca gatccatgtt gaatcgatag ataatcatcc
      781 ggttgtatta agtcttggtg cctgtttctt tgatgggaaa gaagatttaa gttttgatga
      841 attatattgt agagcggact cagctatgta tgaaagtaaa aaaatcgacg gatatagtgc
      901 aacaattttc agaaaaaaat agcctctttg tgaacaaaga ggttgttttg tacctttaaa
      961 gtagacaagt gttaaaaaat agtaaacatt tattgaattt tgtggtatta tacccgaaac
     1021 ggagggaaat atatatgcag aagtatgtag aaatcaagcg agatgattta acacttcgtg
     1081 gtatgcttca tattccaaat gatgtaagcc aaaaagtgcc tatggtcatt cttcttcatg
     1141 gtttttgtga tgatcgaaat gaaattaatt ttgtacataa tgaattgtct caaagactat
     1201 gtgatgctgg tattgccagt gttcgttttg acatgaatgg cagtggtgaa agtgatggaa
     1261 gatttgaaga tatgactgtt tcaagtgaaa ttttagatgc acaagccatg ttgagatatg
     1321 ttcgatcctt ggattttgtc gatactaaaa aaattgcact tcatggatgt agtctaggag
     1381 ggtgtgtggc cagtatggtg gcaggaaagt gtaaagatca gattcaagct ttatctttgt
     1441 ggtgtccagc tccagattta gtttataact taaaggagca taagactttg tgcaatcaag
     1501 atgtttcaaa tattgaaaaa gatggatgtg ccgatgtaga agggttaaaa ttgagcctaa
     1561 agttttatca ggacgcttgt acattagatc catataaaga agctagtttg tttgataaga
     1621 atgtatgtac gatacatgga gatcaagata ttacggcttc ttgtgaatgt agttataaat
     1681 ataaagaaat ttttaaggaa agagcaaaat gtattattgt caaaggggca gagcacagat
     1741 ttaaatcgtt tgcgtttaga gaagctagaa tgcaaggtgc tctagacttt ttgaaagaag
     1801 aattattata aagaaagtga aggaatttga tatgaatcat gaaagaaaag agccaacaac
     1861 actatttgcg attgtttcat ttttggtaat tattaccgta cttattatta tgatcaacat
     1921 gggatgtaat acaacactat ctgttttttc gggtgttatt gtatctgtga tcacaactcg
     1981 attgttgaat attccatgga atgaagtgga acaaaatata attaaaacgg ccgcagaatg
     2041 tatgcctaca tttttaattg tgattatggt gggtatgctt gtaggaattt ggatggctgg
     2101 tgggactgtt cccacattga tgtattatgg tatgcaagtg gtatctccaa aaatattagt
     2161 accattagct tttgtgcttt gtgcactcac aagtgagttt acgggaactt catttggttc
     2221 agttgccaca atgggtcttg cgatggtagg aattgcctct acgactagta ttccggtacc
     2281 attggtagtg ggtgctgttg tttctggtgc atggttaggg gataagatgt ctccattatc
     2341 cgatacgacc aacttggcaa gtggtgtaag taaagtgcct ttatatgatc atattcattc
     2401 tatgcagtac actacgcttc cagcagctgt agttgcctta gtattattta caattgcggg
     2461 gctttattat agtggtggaa gtatggatct tgcccaacaa aaattaatta tggatacttt
     2521 aagtcaacat ttcacaatta atattttatt gattttacca gcaattttag ttattttgat
     2581 ttctgtattt aaacttccat ctttgttagg tatgggactt gcagtagtag tatcggctat
     2641 ttttgcgatg gtgtttcaag gtgtaaattt tgtcgattta atgaattatg cttttaatgg
     2701 ttttagtatt gaaacaggtg tatccattgt agatccaatg ttaaatcgtg gtggtgttct
     2761 ttcaatgtct gaattgttgg ttacatttat ggtggcaagt gtaatgggcg ctgtcattac
     2821 gtcttctggt attttggatg ttttagctaa aaatgtttta ttgaagttca taaagaatcg
     2881 tacggttttg gtgtttgtca ctttggtata ttgttacatt gtaaattttt taacagctgg
     2941 tggacaaacc gtatctatta ttgtgacgga acagactttt gaatctacat atgaagatat
     3001 gaatatttcg cgtaaagtat tatcaagaac gttagaagat tctggtactt tgtcagcgcc
     3061 aatcgttcct tggggtgttg cgacaattta tgtcatgggt gtgcttggat gtagtacggc
     3121 atatattcca tacgcttggt ttatctttat tgttcccgtt ttctcaatga tttgcgcaat
     3181 cactggaatt ggtgtttggg ataaggaaga aaagccggtt cgaaaataaa tattatgcaa
     3241 ctttactatc ttttagaaat ggtgtataac tttaatgtaa ataaaaaaag ggagcttgta
     3301 gtacaggctg agaggaaagt atgactttcg acctataacc tgatttggat aatgccaacg
     3361 tagggaaata ctttgtcttt tttagtgtga gtatacctat attggttgct cacttttttt
     3421 agtggcaaga tgggagcacc actttttgtc acgttaaaaa atcatttgga aaaggagaat
     3481 taaaaatgaa cacaaatgaa agtacagtaa gtacaggcgc tacatatacc actcagatgg
     3541 atgctgcgcg taaaggtatt attacgccag aaatgaaaat cgtagcccaa aaagaaaaaa
     3601 tgacagagga agaattaaga gcgttagtgg cttgtggaaa ggttgtaatt ccggctaata
     3661 aaaaccacaa atctttagat cctgaaggcg ttggtagtat gttacgaaca aagatcaatg
     3721 tcaacttagg tgtttctaga gattgtaaag attatgatgt agaaatgaaa aaggttatgg
     3781 ctgccgtaga cttaggtgct gaatctatta tggatctatc aagtcatgga aatacacaac
     3841 cattccgtca aaaattaaca tcagaatgtc cagctatgat tggtacagtt cctgtatatg
     3901 atagtgtgat tcattatcaa agagatttaa atacattaac tgcaaaagac ttcttagatg
     3961 tagttgagtt gcatgccaaa gatggtgttg actttgagac tttgcattgt ggaatcacaa
     4021 gaaaaacgat tgatcaaatt aaaaaacata aacgtaaaat gaatatcgtt tctcgtggag
     4081 gaagtttagt gtttgcgtgg atgtcaatga cgggggaaga aaatccattc tatgaatatt
     4141 atgatgaaat cttggatatt tgcgaaaaat atgatgtaac gatttcttta ggagatgctt
     4201 gtcgtccagg atgtttagca gatgcgacag atgtatgtca aattgaagaa ttggtgcgtt
     4261 tgggagaact aacaaaacgt gcatgggatc ataatgtcca agtgattgtt gaaggacctg
     4321 gacatgttcc tttgaatcaa gtagctgcaa atatgcaggt tcagcaacaa atctgtatgg
     4381 gagctccatt ctatgtgtta ggcccattag ttacagatat tgcgccaggc tatgatcata
     4441 ttacggcggc tatcggtggt gctgttgcag ctatgtcggg tgcagctttc ttgtgttatg
     4501 taacgcctgc agaacatttg gctttaccaa atgtagaaga cacaaaacaa ggtattattg
     4561 cctctaagat tgcagctcat gcagcagata ttgccaaagg tgttcctgga gctagagata
     4621 ttgatgacaa gatggctgat gcaagaaaga atctagattg ggatgctcaa tttgagtgtg
     4681 cgctagatcc agaaactgca aaagcaattc gtcaatctcg tatgccagaa gaagatcaca
     4741 gtgatacttg tagtatgtgt ggaaaattct gtgctgtaag aagcatgaat aaggctttgt
     4801 ctggagaaaa tattgatatt ttgtaacgtt tgtacatttt agtaagggtt tgtaataatt
     4861 aactgaattt agtcaagagt ggatagaata tgatatatta aatatgtagc tttattgtta
     4921 gattcatcaa taacaaacgt taatgaatga cgtatgttaa ttatgattca atagacaaca
     4981 agttagaaag ggtaatttat gagttagttt gtattagatt tttttgaggg gaagaatctt
     5041 gcgacgatcg aaaaaaagaa gcattcatat ttaatgatag atggtcatga tcaggcagat
     5101 atttatatat taaaagatgg aatagtaaaa acgagtatta tattaagtga tggtcgtgaa
     5161 tttaacatta tgtatttaaa aggtttcgat gtgctatcac ttttaaaaga tgaaataaaa
     5221 aaggttactt cagcatcttt tcatattcgt attgaaagtg atacggctgt attctatcgt
     5281 gtgaatcgta aattgttttc aaaattttta agtgaaaaca aagaattaag agattatatg
     5341 gatgcatatt atcgtaagaa gttgtcggaa gccatttatc gccagcaatt aatgactatg
     5401 aatggtaaaa atggtgccgt atgtgctttt atttattatc tggttggatt attcggaaga
     5461 gaagttaaga agggcatctt tattgatctt caagttacaa atgatgatat tgcaggtttt
     5521 tgcggtgtat ctacaagaaa tagtgtaaat cgtatcttac gtggattaag acaacaaggt
     5581 gtcataacaa tggtatatca taaaatccta gttctagatg tggattattt aaagcgctat
     5641 gtatagtaaa aggagcaatt cagctccttt tgttgtttaa taatgtcatg tacatcaata
     5701 attgttttct tgattttaca ccaagtttac tataaatatt acgattgtga tactttaatg
     5761 tattgtccgt aatatttaat gtttctagaa tatccttact tgaatatccc ttcacataga
     5821 gatttaaaat ctctttttct ttacgtgtta aagtcgcaag acattctaag aaacgtttat
     5881 attcatcctc atcaatatca tcgatacgtt ttgtcgttaa ccgctccact tcttttttag
     5941 ctctgtcata ttccttttga atggcttcat gttcattttc aatctgttca agcttcttgc
     6001 gattttcctg catttctagc tcaagcgtca acattttatt ctcaacttca tagtcttttg
     6061 aagataagaa atccaaaaga tcatcaattt cttgtacatc atagcgcata ctgtttttat
     6121 tgtctttacg cagctgttct aatcctttta gaattgggga caggtatctt tgactgaaga
     6181 aggagcatcc taaaaataca aataataata ggacaatggt aaagaagaat aattcaatat
     6241 tactttttgc gatggctcga ttgtagtcag aatccaaaat cataacatat gtcgaatagg
     6301 tttgattctt agaaattttg acagtattct taattccaat ataattacga tttttactta
     6361 tgattctcaa taagttgttg ccaataggct taatggaaag atctccattt ggcatataat
     6421 agaatccgtt ttggcttccc gtactaaagc cttcatttgt gatgattgaa tcggagtctg
     6481 gcactaaaag acacgttaaa tggttgagag ttgagggctg gggatgcgca aatttaaacc
     6541 agctttgtga aatttcaaaa ccacaaagac catagaaggt accatccttt ccaactaaag
     6601 gaacacgtaa tagggcgatt ctttcacctg tgtatggcaa ttgaataata tttgataggg
     6661 aataatcaaa gttccctttt tttaattctt cataatcggg tacaagtgta atatcaaatt
     6721 cctgtctcca tttacgatgt ggcatgatgc catgtttttt tcctatattt gcgatgccac
     6781 gatacaaaat taagtcttcc tttgaatagg acattgtcgt tttttgaaga tacacaccac
     6841 ttcgttgtgt ctgcgcatta tccaaagtgg aattgattgt cgtatctagt tgtacataag
     6901 ctcctgaaca attagatcgt tcaagatatt gtcctaaagg ctgaatcata tcgttttcta
     6961 aagaatagat ggcattggga ttgtcctgca actgttgaaa ggttaatcct tgattcgcta
     7021 gggttgtttc taatatagct tccatattat cagccaatgc gatattcata gaggccaagt
     7081 catcccaata attttccata ttctttgtga agtattcact ttgtgttgtt agatctttat
     7141 gaaaatcttg ttcggtcgtt ccaagtcttc caaataggaa gagtcccatt aataaagtaa
     7201 tactgatggt agtcactaga aagaacatat atacgaaaag ctttcgtttc atggatcttt
     7261 cttttgattt aaaacttaat tgcataggct aacctcaaga aagacttttt aaaatattca
     7321 ttgcttcatc tacaatttca tcaatatctt caccggcatt agctcgtgct tgccattctt
     7381 gttggtggct tcttacttga tcataaaatg cataggcctt tctagaaatc gcatttggaa
     7441 cctctctaga aataacggta tagtcggatt tcatttcggt gagtgtattg tataaagatt
     7501 gtgtttcttc atcttcaaac ttttcatttt ttaagttttt aaatgctttt tttgtgactg
     7561 gcatgtaacc tgcttggatc acaaagtcca tgtttcgttt tggttccaca agccaatgtg
     7621 caaagacact agcggcttct tgtttttgtt ctgtagtctt ataggcacat aatccaacac
     7681 cagcttgtgt catgagggca tggtctttat cggcctttgg ataaggcagg gcaatcgcat
     7741 ttaatggttc cgttgtatta tctggatatg taacgatgtc atttaaatag agataggctg
     7801 cactgctgga tacaccaatg agtgtttctc cggttgtaag ttctgtgctt gaatagaaat
     7861 ctgaagcaga aatatggcct ttgatcaatg gttctaaaaa agttctaaag gcttcttgaa
     7921 attctttatc gtccgtatta taccagccat tctcattata catatgttta gagcctaatg
     7981 cgattgcata agattcaatg gaatttaata gataatcaaa gcataataac ggctttccat
     8041 ttgaccattc ataatatttt tcggctacat caaacgtttg attccaagtg tcaaaatcat
     8101 ttaatgaaac agcggtatcg ttagaaaact tttcaaattg tgtaccattc ataaataaaa
     8161 cgattgttga ttttgataat ggaagatcca caagcttatc ctctagcatt ccagcatcta
     8221 aaaactcatc aacaaaattt gaaatttcct tctctgtaaa tacgtcattc catacaacac
     8281 tcttatctaa gcctacctgt ttggcattat gaatatgtcc tgtataaaga tctggcattg
     8341 gatctgcacc tggttcttta gataaacttt cttcaagctc agaaccaatt ttactcgcat
     8401 tggttatttt tgtgacatta attataatgc ccttgtcctt gccttctgtt tcattgaatt
     8461 cagcaatcaa ttgattcatt ggagaattgg cttgttctcc ataaacatgc cacattgtca
     8521 gggtcacggg gttatcttta tcgagtaaag attttgatga acacccatat agattgcata
     8581 ggcttagacc aaatactaat aaatatttta taactttttt catgattttc ccctttattt
     8641 acagcagttc ccaccctttt taagttttgg ggtggggtaa attttataat actttctata
     8701 atgaaaatgt aagttgatta cataagtttt gaaagtgctt tctaaataca atatagcaca
     8761 agtaaacaag tcaatcaaat gaagaaagga gggaaatgaa atggggttgt tttctaaaaa
     8821 ggaatgtgat gtgtgtaaaa agaagatggc attgatggaa ggttataaat gtgcggatgg
     8881 tacgatctgt aatgattgta agaaaaaaat gtcaccatta tttacagact acaaaaacac
     8941 aacaataaca cagatcaaag gtcagctaac atatcgtgaa gaaaacgcac aaagagtcag
     9001 agtgtttgtg gcagctgaaa cgtttggtga gtatccaaag gtaatggttg atcgaaagca
     9061 caactggttt gtcgtgacaa aggcaacaaa gcttagtgat gaaaatccag atgtgtttga
     9121 cttaagtgac attcaaaatg ttttggtaga aattgaagag aataagcgag aacttcattt
     9181 cacaaataag aaagggaaga catgttccta taaaccagca agatacgaat ttacgtacca
     9241 attcaaggta aatattcaaa tgaatcatcc attcatcaaa atgatttctt tgaagattac
     9301 caaagaggat gtcaagatca tttcagaaat gcaggataaa gaagtaatgg tacagtcaaa
     9361 cgaaaagtat caggaatctt tacaggtggc aaacgcatta aaggatgcgc ttgaatcata
     9421 ataatgcaca gaaaaggagg aattatatat ggcgaataca tgtgcaattt gtggtgcaac
     9481 aattaatgtt ttacaatcac agaaattaat ggatggaaat tatatttgta caaagggttg
     9541 tcgtgcaaaa ggattaaaat attatgatta tgttcattca gatttagata atgttaagga
     9601 tcatattcat cnnnnnnnnn nactgaggtg ggtacaaaga tttggcaaga tttaatggaa
     9661 cctttgaaaa aaacgcgtga taaaaatcaa aagatgcagt ctttccaccc tatttatatt
     9721 gcaccaagtc taggtttgat tgcggttatt gaagctcgtg gaggtttgtt taatacaaaa
     9781 acatatgcgt gtgtatatcg tctagaaaat cttcagattt ataaaaccga aaatatgcca
     9841 gctagaacga gtggttcgga taaagataaa aagtgtgttc acttaggctt tgtacataca
     9901 aaaggtttaa atgatgtcta tattccattt gatgatgaaa ctagatgcca tcaatgtgtg
     9961 gattatctaa ataaattgtt tggcttagat gattcattta gaagtggcat taaaaagtct
    10021 gtaactcaat ttaaggcaac aaagagtatg tgggatttag cgaaagctaa aaagaatgga
    10081 gaaagtttag aagaaaaagc gaaagcgaca gtggatgcgt ttggggcaac gatcattgga
    10141 gatcgtacgc aatttaaaga tgcagccgat caagctcttg ctggatatga cttggattaa
    10201 gctttgttcg gtcttgtttc ttagtttgaa tctaatgttt acaggttgta aatcacaacc
    10261 taatgaagag gtatatattt cagatttcta caatattcat gaagaatata aggatgcatg
    10321 gggaaatgtt ggtaagtacg acatttcctt accttatatt cattgtgatt caaagaacgc
    10381 aaagaaattg aatcagacaa tcaaaaatga atacaaggat ttggtagatt ctttagatga
    10441 ggcaaaagaa gaagggtata cgttgccgta cacaaaggtt agttatgatg tgtatacaca
    10501 ttcaaatcta ttgagtttag ttattaagga agaagtagag acggaatatc ctaggtataa
    10561 ggtgtatcat tttgattcta aaacgaagaa gcgtgtttcg aataaaaaat tgtataagaa
    10621 atataaaatc agtcaaaaag atatgaaggt atgggctgca caggcttttg atcaggaata
    10681 tgccaatgaa tattatgaca atgtaaagga gttaagagct caaacaattg gtttgttgcc
    10741 ttcaaatcta gatgtgtttt accacaaagg aaaattgaat gtattgattc ctatcgccac
    10801 aaattttggt tctggaacaa agatggtttt gtataggcca agtcgtaaga agtcttataa
    10861 gatagaagat tcttccacgt ttgaccagat gactatgaca ttaaagaata ataagctatc
    10921 gctttactat agtggaacgg ataaaacgta taaggtgcat ggttgtttta atgagtataa
    10981 acaagttcat gtgtatgaaa taggtccggg tgtcacttat ggttttgcac taacgacaaa
    11041 tggtttggtg gaagtgattg cgattgagga tggtttaccg attgagcgtg tttgtagtca
    11101 aggaccatta tatgggttag ataaagtaaa aagccttcgt agtgggcagg cactgacaga
    11161 aaatgatcag ttgattgatt tatttgattc attgcaaaat cagccgttta aaaatcttga
    11221 gggtacgtat acaaatgaga gtgtaacact tcaaatcgat gagaatggtt tgtatgcaat
    11281 gcaggatcaa gagaattcgc attctggcct attgtttagt gtaggtatgg aagaagatgg
    11341 atttgtgtat ggatatgatg actataatca acaagtctta tcgtattgta aagtaatgcc
    11401 gttatatgaa aaattagagg tcaaaaattt gtttgatcat gacaaattag aattggagta
    11461 taaatatgaa taaaaaagta ttagtaagtg cattcgcatt aagtatggta tttggattga
    11521 caggatgttc aagtgaaaag ccagaagaaa aaaaggaaga tacaaaggtt gttgaaaaga
    11581 aagaagagaa gaaggaagaa aagaaagaag aaagtacgga tgctgtatta gtaagagaaa
    11641 tttcgagtaa gacatataca gtcacaattc cacagcttac ttcattctat acaccaagta
    11701 gagatggttt agaagttaaa acagaagtaa atgagcaaca aatctcatat acacttgaag
    11761 atgaatttgg tgattttaaa atggctattt atgtaaatcc aatcagtgct gagagtgcag
    11821 aagcctatgc gaatcgtatt aatgaaggat ttaaggatga tgaatcgaaa caatataagc
    11881 ctagcacggt gggtgatttc agtggcttta gaaaagtgac tacaagtgaa aatcctagct
    11941 tcaaatgcaa agataacttg ttgttggatt ttgaaagtgt ggatggttct gctgtagtga
    12001 tgtctgtaac tgtagaacaa ttcaatgatt cggatactgc ggatatggaa gtagcgatga
    12061 aagctatttt gggcaatatg aaagttgagg ttaaatagtc atgaacgcaa aatttgtgaa
    12121 aagcttatta gtaatcggaa tgggcttgag tttagctgct tgttcatcga atgatccaga
    12181 gcctaaaaag gaagatacaa aggttgtcga agctaaaaag gaagaaaaga aagaggaaaa
    12241 gaaggaagag aaaacaacgg attttgtggt gagcaaaaac tacaataaga agtatgagat
    12301 catcatcaca atgcctcaag atttcacaac gatttatacg ccagcaaatg gtgtagaggt
    12361 tacaggagag attgtcaatg attcctttat tcgctatagc tttgaaaata aagaagccga
    12421 tacgaaaaag attgcggcta ttgaagcaaa tgcagatgca acagcggatg aagtggctga
    12481 aaccttcaat aaaggtgagg aagatgagag taagctttat aaggcttatc aggttggaaa
    12541 ttatacaggt gtaaataaga tttcggatta ttcttatgat gattatgtaa cgtacaatat
    12601 gtatttcaat ttggatcgag atggttttaa aggaacaatt aaggttgttg ttgaaaagct
    12661 aaaagcggaa gcggatgatt cggatacaga agctattatt actgcaattt tagataatgt
    12721 taaagttgaa aagaagaaat gtgagtaagc ctatgaatca taagctagct aagatcttgc
    12781 ttgcgctagg tataggctta agcctagttt cttgtgcaaa aaagccagaa gaaccaaggt
    12841 cttcacaagt gaaaaaagaa gagaaggtaa agattggcga aatgactgga gtcgcacaaa
    12901 tgaatggcta tttcattaaa atgacgatgc cggatttatt gtcggtttat acaccaaaac
    12961 aagagggctt agatatatcg ggtaagctag agggtggtcg tatccttaaa tatgatctac
    13021 attcggatga tcgtacgtat attacgcgaa tttcaattga ttgcgattat cgtaaggcgg
    13081 ccaaggaaac ggcggatgtc ttgaatgcgg gttttgaaga tgaggcagat caatataagg
    13141 catttacaat tggtaagtat tcgggttata agaaaacaac gatatatgat tcgtctgata
    13201 aaacctattc taagatgttg atcgattatc cggttggtga agataatgtt gtcttacaaa
    13261 tctatgtaga acaagaaaaa gccaatgatt tggatgaggt aacaagagcg attttggata
    13321 atatcgttgt ggaaatttgt atggaagagt aggtgatcag agtgaaaagg aataagttat
    13381 taactggcct cttggttttg ggtatgggac taagtttagt ggcttgttca tcaaatgagc
    13441 cagaagaaaa ggtagaagaa aaaaaataga agagactaag gcggctgaag aaaaagaaga
    13501 aaaggtaaaa gaagagaaga agaaagaaga aaaggctaag gcagctgagg aagaaaaaga
    13561 aaaggatagt acgattatta gttatgcgaa taaaaaggta agagttactt tacctaaaga
    13621 tttaacttct ttccatacac cagctagaga actttctcga tatagctata tggaagaatc
    13681 gaatgttctt ggttattcat tatatgcgaa agatcgtacg ttcaccattc atttctcaat
    13741 tggaacggat ggctgggatt cagcagaggc tgccagcatt tctcacaaca aggtttgtaa
    13801 ggatgaatca aaaatgtatg agccatgtac tgtaggtgaa tattctggat ataagtatgt
    13861 ggtgtcaagt gataaatcga ttgaatatca ttatgtgctt gattatctag tggatggtca
    13921 agatgtcgta ctaaccattc gtgaagaatt acgtaatgat gacgatactt ccttgttaga
    13981 gccattgttg ctggatgtgg tacataattt aaagattgaa ttaataggag aataatatga
    14041 atgggaataa gttattaacc ggcttcttaa ttttgggtat gggactgagt ttagtggctt
    14101 gttcaaatga aaagccggag acaaaaaaag aagaggctaa ggtagcagaa aagaaaaaag
    14161 aagagaaaca aattgttacg caggatcgaa cagctaaatt gagtattgtt gaagatttat
    14221 ctccttattt tacttcatct agagagggtg taggtaaaat ggagggtatg ttaaaggata
    14281 atggttcgat tgtctataag tgtttaaatg aagagaataa gtatgagttg aatgctgaaa
    14341 ttaaattgtt gtcgtctagc tctgctgaaa agagtgcgga tctacagaat tcaatgtatg
    14401 gtgaaaacga tgaggataaa aagtataagc cttgtacgtt tgagggcttt gatgcgtata
    14461 gtcacaccat caatctgggt acagttgaaa atactagact tcactattat gtagattatc
    14521 cagtcgagga tatcaataag gtcgtttcta ttgagatcaa ccagaagaat gataaagaaa
    14581 aggatacttc tgatttagaa ccttttgcga aggctatcat taagaatatg aagctagaaa
    14641 aggtgaagga agaaaagaaa gaggaagtaa agcaggatgt taaagaagag aaggtggatg
    14701 aaaacagcaa ggaaattgtt ggtgaatcgt ctcgtgcaaa attaacttta ccaagggatt
    14761 tgacttcgtt ctatacgcca aatgaaaagt atgacacgat caagggtgag caagataagg
    14821 gaaacattgg ttatacatta agtcgaaaag atccagatcg ttcaatatac gtaaacatat
    14881 gtacggattc aagtgatacg cttgaagagg ctatcgtatt ccataatgaa catttaagta
    14941 aaggagaaga accattcaca gagtatacat tgggtgaata ttctgggtat cgttctatga
    15001 gctaccacaa ttattggaat agtgccaatg atacgacgta taggtataag ttaaattatc
    15061 cagtaggcga taaaaatgta atattgtcat gttctgtaac acttcgtgat aattcggatg
    15121 acacgacagg attagaagat atattattgg cggctttgca gcaattgaag gtagaaatta
    15181 aacaataata ggagaacata atatgaaaaa taataagtta ttaacaagtc tgtttgtgct
    15241 tggtatgggt ttgagtttag ttgcgtgtgg tgcaagtgat ggtggtacga ctacaaagac
    15301 ggatggtaca aaaacggtag agaagaaaga ggagcagaag tttgcggatc gaaaagagta
    15361 tgaaacggag ggcctagctt gtaattttaa attgccaaac ttggattctt tctatttggt
    15421 gaagggttta gaaaagcctg tttataatac ggaattatcg cttggaaaca caaaatatga
    15481 agtttatgat gattatcttc cggttctttt agtatatgta ggagatgatg cattcgcaga
    15541 atggagtgat ggatatacta ttgaggaact tgcgacaaaa gatttgaatg gggaagctga
    15601 atatacagag acaaaagtcg gtggttatag agctttagtt gcagatttat cggatgagga
    15661 tagtttaaag aaagaatact atattgaaca tccagatctt ccaaatggaa tcgtttgtat
    15721 tgaaatcaat gaatatgatg ataaggatat ttcagatgaa ttggcagaag cgattattaa
    15781 agagttcaaa gttgttgaac ttttagatgt tgaataattc tactttatgt cgaatccgaa
    15841 tgatggatat gccaaaagaa aagagatgag tgtacaagga ttggcttgta cattcactct
    15901 ttcgaatatg gatgcttatt ttcttgtaga ggatgagaag gtttcgtata aaagtaaagt
    15961 tgttgcgtct ggtatgatgt atcgcatttt tggtcagaat atggatattt tagtgtttgt
    16021 ggatactatt ccttatattc gaaacaatgc gatgtcttat gatgaagtgg ttcgtgttga
    16081 gtttgaaaat ctagattata aagatttaga gatacttgct tataagacat gtatatatga
    16141 ggaagaggat ggtaattata taaaacgtac atatcttgtg gactatccag atatatcaag
    16201 tggtgtattg agaatcgaaa ttctgagtaa atcgaatgaa tttgattctt tagtaagagc
    16261 gattgtaaaa gaaatacaag taaaaacaat agaagaatgg taggtaaata atatgggatt
    16321 aattaaagca ggtgctggag ctgttggttc tgtttttggc gatcaatgga aggagtactt
    16381 ttattgtgat gcattagagc aggatgtatt ggttcgtaag ggttctaaaa ggacgggtaa
    16441 aaagtcgagt aataaaggaa gtgacaatat catttcaaat ggtagtgtga ttgcggttgc
    16501 ggatggtcaa tgtatgttga ttgtggacca gggtcgtgtt gttgagcttt gtgcaacgcc
    16561 aggagaattt gtgtatgatt cttcaagtga accttctatc tttgcgggaa gtttagggca
    16621 gagtattaag gattctttca caacaatggc taagcgtttc acttttggtg gagatacggc
    16681 taaggatcaa agagtgtatt atgtgaacac aaaggaaatg ggtgaagttc tatatggaac
    16741 ggcaactcca attccattta gagtggttgt gaatgaagaa ttgggcttca aattgtctgt
    16801 gaaattgcgt tgtaacggta actttacata tcgtgtggcc gatccaatct tattctatac
    16861 gaatgtttgt ggaaatgtgg cagaggattt tgagtctgaa accattgagg gcaagcttaa
    16921 gggtgaattg atgacggctt tacagcctgc cttggcaaaa ttaagtgcgg atcgtgtgat
    16981 gtattatgag attccagctc acacaatgga tgttgcggca gctttaaatg atgagttgag
    17041 tgatatttgg cgtgctaagc gtggcttgga agttgtatct ttcaatatta actctttgag
    17101 tattcctgaa gatcaagaaa agaagatcac agagtgggaa gaaaatgcga tgacgatgaa
    17161 tcaaacggtt gcggcaagtc gtttggttgg tgcccaggct acagctatga aggatgctgc
    17221 aaataatgcg tctggtgcaa tgactggatt tatgggtatg ggtatggcca tgaatcaggg
    17281 tggtattaat gcgcaaaact tatatgcggc gggtgctgct tctcaaccag ctcctgatcc
    17341 aactacaata ccgactcctg caactacgcc tgaggtaaag gcagatggct ggacttgtcc
    17401 aacttgtgga catctagcaa gtggtaaatt ctgtcctgag tgtggaacgc ctaagccaag
    17461 tgttgagggt tgggcttgtc cgacttgtgg tactgtaaat aagggtaaat tctgtacaga
    17521 gtgtggtgca aaaaaaccgg cacatgaacc gttgtatcgt tgtgataagt gtggttggca
    17581 acctgaggat ccgcatcatc caccaaaatt ctgtccggag tgtggtgacc catttgatga
    17641 taatgacata gaaaagtaga gggttttaat tatgagtacg ttacaagagt ttaaatgccc
    17701 ttgttgtaat ggtgccattt cgtttgatac aaaacttcag aagatgaagt gtccttattg
    17761 tgatacagaa ttttctctgg aaacacttca gcaatatgat tctgtaaaga ttgaggaaga
    17821 agatcaattt gagtgggatg tgaagccagg tcaagagtgg gcagaaaatg aaacggatgg
    17881 attattgaca tatgtttgta attcgtgtgg tggtgaaatt gttgcggatg aaaatatggc
    17941 ggctgcatct tgtccgtttt gtggaagtcc gattgtcgta aaggaaaaat tgggtggtat
    18001 cttaaagccg gatttagtga ttccgtttaa attggataaa aaggcggcta aggaaggttt
    18061 gaaacagcac tataagggta agactttatt gcctaaagaa ttcaaatcag aaaatcatat
    18121 tgaagaaatt aaaggtgtat atgttccttt ctggttgttt gatgcggatg ccgatggcga
    18181 ggttcgttat aatgcgacaa aggttcgtat gtggtcggat tcagaatatg attatacgga
    18241 aaccaaatac tatcgtttat atcgtaaggg gcatgtaaga tttgaaaaca ttcctgtgga
    18301 tggttcgagc aagatgccag atgatatgat ggagtctatt gagccttatg acttaaaaga
    18361 tgcggtggat tttcagacgg cttatttagc agggttttta gccgataaat atgatgtgga
    18421 tgccgattca agtattgatc gtgccaatac ccgtgtaaag aagtctacgg aagatgcgtt
    18481 tatgaccaat tgcggtaagt atgacacggt tacttgtgag gatagtgcag ttcagattca
    18541 aaatggtacg gcaaaatatg cgttgtatcc agtgtggatc ttaaatacga agtggagggg
    18601 tgaaaactac agttttgcga tgaatggtca aacgggtaaa tttgtaggga atcttccaga
    18661 aaataagagc ctggtttact tatactatgt agtggttacg cttcttggaa gtgttttaac
    18721 ttatatcgta atattgattt tgagttgggg aaagtaggag gttgtttatg aaaaaactta
    18781 gtattttatg ttcatctctt ctacttgcac ttatgatgtg cttcccggtt tttgcgagtg
    18841 aagatctaag atatgtagag gataatgcag gcttattaac tgtaagtgaa cagcaggaat
    18901 taaatgatac tttaactgaa atcagtgaaa gacaaggtgt agatgttgcc gtaatcactg
    18961 cagatgatat gggtgatcaa tccatagaag actacgcaaa agcaaaatat gatgagcgtg
    19021 gctatggtaa tgatggtgct ttattggttg tcaacatgga agttcgcaag tggtggatgt
    19081 ctacttatgg aaaaggtact actgctattt cgtcagaaga cattagtacg attggtagtg
    19141 agtttgcgcc ttatttaagt agtaaagaat atgcggatgc atttgaaaca tacgcaagac
    19201 tttgcgatga ctatattact agagcaccta atctttccga agagtatgcg caagctttaa
    19261 gtggtcttac ttcattacaa gatggactaa gatttgtgaa tgatgatgcg cgtttattaa
    19321 ctgaggatga aagaaaagag ttaaatgata ctttaaccca aattggtaag cgtcaaggtt
    19381 tagatgttgc gatattgact acaaaagata cgggtggtaa ggcacttctt gattatgcaa
    19441 tgacgatgta tggagcgctc gagtatggtc agggtaaaaa taaagatgga attcttttag
    19501 tggtcaacat ggaaacacgt aagttctgga tggctactca tggatatggt attacagcat
    19561 ttacggatgc cggaattagt tatattagtg aaaagctagg cccttctttt aaaaaagaga
    19621 agtacatgaa ggcttttaca acgtttggta gtctttgcga aaagtttgta gaaaaggcac
    19681 ataatggtaa gccttatgat gttggaaata tgccatttaa atggcttcca tggtactgtg
    19741 ttccaataag tcttttagta ggttttgtct gtgcagtctt tgtggttagt aaaaagcgtg
    19801 aacagcttca ttctgttgcg tttgaagaca aggcacataa ctatatgaag aaaaatacgt
    19861 tgaagatgaa agagtctcat gactacttct tgtatcacac gataactcgt aatcgaatta
    19921 aatctgacga tgatgatagt tcgagcggag gaagtagcac atattctagt agtagtggtg
    19981 acacgtatgg tggtggtggc ggtagcttct aatgccgcaa aatgcaggat caaaccctgc
    20041 attttccact tggttcaata aaaaaaggag gaaacacaaa tgatgaagca atgtatgagt
    20101 gttggtctag ctttaagtat gttgttgtct ttaactccga taacagcaat acatgcagat
    20161 gaaacgcaag aattagatgt acaagataca gaagaaagaa aagaagattt agagttagtg
    20221 ccatcggtaa gtgtggctgg tttaaaggct gtagctactg aaaaagaatc aattacaatc
    20281 acgtttaaaa atgtaggcca acaagtcaat aatgataatg agaataattc gttttgggga
    20341 gaaatatatt ttacagattc tgctgggaag cctatcccaa acttggattt tgaacttcat
    20401 tgcgatgatg tgacaactca atttgtaaaa tcagatgcaa acgggaaata tacatttaaa
    20461 tatgtttata gtaaacctat gtatactcat agaaatcctg ttagagtata tgccatggta
    20521 cgagaaaacg ataagtatgt tcatagttat ggggaacaat attatcataa tccaattaaa
    20581 tggtttcatt ttataacaca tgaagatgaa aagtataaaa ctgccacttc cattacatat
    20641 ccatttaatg tcacaggtgg agaaattgtt gaagaaattg aagttttgaa aacatatgag
    20701 gatggaacga ttgtatttga tgcttggtat gatgatgaag cacgtacgat taagcatgat
    20761 tttactaagc caatcataag caatgtagat atttatgcaa agtggaaaaa gattgaaaaa
    20821 ccagttgttc ctcctgaacc agaagtagaa agagtcaaaa taacatactc tccgggtgaa
    20881 ggaaaagggc ctaatgcagt tatgaagata ccaaaatcgg atgaggatta taaagtgcgt
    20941 gattttgaat attcaccaat aggaaaaaca tgttcacctc cttctgataa gtattacttt
    21001 aaagagtggc ttgtaaaata tgcggatggt accgaaacaa gagtaacacc tggtactaag
    21061 atcaagatta ctcaagatac agcttttata gcgcaatggg caagactata tgaagtaaag
    21121 gttgcggata caataacgaa tggaacggtt gtcttgatga ataatgatag agagaaagga
    21181 caggttgtta cggcaaaaga aggggaaact attcttgcga ctttaacagc tgataagggt
    21241 tactacaata aaaatatcat aattcagcaa gcaaatggta gttatcttcc gtggaaaaga
    21301 aatgaattta atccttatct tgtaactttt aaagtagaga atcatgatat tacatttaat
    21361 gcaaattttg catctttaac ggcagatcct gaaaagccaa taattaaagc tcgtaacttg
    21421 aagtataacg gtaaagaaca agaagtagaa gtctttggtt atgattcagt tatgatgaaa
    21481 attgaaggta ataaggcaaa agatgctggt acatatacag tgaaggtaac atcaaaaact
    21541 ggtaaatggg atgatggttc aacggatgct gtgacaacga aatggaaaat tgaaacagta
    21601 caaagaccag ctcctactgg attaaagggt tttgatactt catatcctgg tgcaaatgat
    21661 ggaaggatta cgggtgtaac ggatgaaatg gagtatattg aagatacgga tacaaagttt
    21721 tatccttgta caggtacaga aattacaggc cttaaaccgg gattgtataa tgtgaaatat
    21781 aaagatgata aaaatcaaac attaaatatc tctgcccaaa ttgaaattaa agagggtaca
    21841 ggaacagttc caacacatat acatacttat ggtaaatgga caagtgatac tacatctcat
    21901 tggcatgaat gtacggatgc ttcttgtagt agtataaaag acaaagcagt tcatgtatat
    21961 gacaatgatg cagatacaac atgtaatata tgtggctatg ttcgtacaat tacacc
//
LOCUS       DBMDWL010000012        21498 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_883_length_21498_cov_2.394588, whole genome shotgun sequence.
ACCESSION   DBMDWL010000012 DBMDWL010000000
VERSION     DBMDWL010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 21498)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21498)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21498
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_883_length_21498_cov_2.394588"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>317)
                     /locus_tag="ACZPDN_01570"
     CDS             complement(<1..>317)
                     /locus_tag="ACZPDN_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013577.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1297345.1"
                     /translation="NASASSIIRIVQKLGFKGYSEFRYFLRNELDRKDRANVINTNKI
                     GSSVVLEDVTSTIRLFEQNKFIEDIYKLMATSNRIYAYATGYGQSLMLKEFSRCLINV
                     GIH"
     assembly_gap    318..327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            730..2313
                     /locus_tag="ACZPDN_01575"
     CDS             730..2313
                     /locus_tag="ACZPDN_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014437.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1297346.1"
                     /translation="MRTFIQKLGKSMMGPLSIIVAAGLLLGITTILTNASLVGEAFAS
                     AAGVQVVVGAVSALVGKVFGLLPVLFAISVATGMVKEDKEIAGFAVVICFVLFHVMLN
                     YMLGLNGITAENTAIDYLTSHGSSTLEAYKINAAYDTQLGIFTYRMGIFGGIVTGLWT
                     AFIHNKFHTQKLPVAFSFFAGNRFVPIMIILTVPFVAIVSYFVWPFFSMLIDGLGTVI
                     SKSGPFGLFLYGFAERLLIPTGLHHVLNQLIRFTPVGGTAMIDGTVVSGALNIFQAEL
                     VAKNMDMNVFHQATRFLTQGTHPFMMFGLPGACYAMYKTAYAKEQPKVRGMLLASALT
                     SFATGITEPIEFSFIFISPILWVFHAFMAGMSFLIMSLLQVGVGNAGGGFVDLFIFGI
                     LQGTYTKWYFCVILGIVYFFAYFFVFKYVIEKKNLKTPGRDVEDSSDDVGQVEASELG
                     TTILKAIGGKENIRDIDNCISRLRLILNDTSVVDEAALKTTGSLGIIRINENNIQVVY
                     GPKVEEATHALKQAAGTYK"
     gene            2374..3744
                     /locus_tag="ACZPDN_01580"
     CDS             2374..3744
                     /locus_tag="ACZPDN_01580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1297347.1"
                     /translation="MEVKYENVKLPECDCTNVPVQLTDETMQERLNKVLDRMNRENFD
                     SLVIYADLEHGNNFEYLTGFLPRFEEALLVLNSNGNHYMVLGNENLNKASKARIKNTS
                     IHCPHFSLPNQPMDVNEGIKDILKRCEFKSGDKVGIVGWKNFTSKYEDNAQLFDVPYY
                     VVDTIKELVGSNVFNATRLFIGEDGARCTNNVNEIVHYEFGAALASDCILKAMNHLDV
                     GVSEMELGNILNAYGQRNSVVTIAASGPRFIKANIYPTTNTVKLKDPISLSIGYKGGF
                     SSRAGYAVHDSSELDEALQDYLDVLVKPYFSSVAVWLEKVHCGMLGGEVYDLIEETMP
                     KAEYNWSLCPGHLTADEEWMSSNIYENSKELVQSGMLYQIDIIPSKSGYAGTSAESTV
                     LLADENLRKQIAQEEPELWNRIQKRRNYIINELNISLNDDVLPMCSTVAYLRPFLLNK
                     EKAMKL"
     gene            3741..3935
                     /locus_tag="ACZPDN_01585"
     CDS             3741..3935
                     /locus_tag="ACZPDN_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297348.1"
                     /translation="MSRKLNSLKSYIINALAFVFSYCMPVVGFFILGYALKKDLPQTR
                     IYASCGVLLAFLTQIVTRLV"
     gene            4234..6132
                     /locus_tag="ACZPDN_01590"
     CDS             4234..6132
                     /locus_tag="ACZPDN_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIR1297349.1"
                     /translation="MIALTLRQRNIIEYLLSRDSYVTNDVIASNFEVSNRTVRKDLEV
                     ISAYLKEQGISLVRKPGKGVWIENKQEVLFDDLSCSYSKNERIVIYVTSLLLNDVNTF
                     MTLASSCGVSKQTVINDLNTIEKFLDGYHLSLIKNQGVGTSLEGDETDIRRLFIDLLF
                     TNDHSKIVYELCINNDGIQRNLDVSFELITVIENEFRFRYVDRDRISIVLSFLLNRLG
                     LMKTKNSTIQSSDMERINSILSTKISDSNDQEYISSFLLGERVEGSLTLDSKVEDEAN
                     EISEHLISSLKKIHYFDSEDMKDIIRSLTVHVRAAIYRCRNNIHIRNELDEQVRFAIG
                     VMYEFTKKELNVYETKYNLNFDQSEISYIALYLASIFETGVQEDLKVVIMIVCTFGLA
                     TSSILKSRMYQIFGDCFIVGPYSIEEAEKYLESHEVDLIISTNYMSVKSTNVIYINPL
                     LKQNDIEMIRNTLFQESFMKMCSLFFNKNENKVVEHDYHCLGEYISKNDVSISKGKIS
                     WEDAIRKAAAPLVAKGDIESKYVNTMIQAVNEFGTYMVITPKVAYVHAGKDDGVNKNC
                     VSLLLLENEIPFGSFNTKPVIAIVVLGICDKEESQLLNIANILDIPENIELLKNSKNI
                     ESILSLHD"
     gene            6146..6583
                     /locus_tag="ACZPDN_01595"
     CDS             6146..6583
                     /locus_tag="ACZPDN_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1297350.1"
                     /translation="MSTGIKLQNIQIGLEAKDWKEAIRIASVPLVKCGSIEEPYVENM
                     ISSVETLGPYIVIMPQFALAHAAPCEYVKKSDISIATFKNDIEFHCDNDPVRIVMCLA
                     CTDKTSHIEMLQSVATTLMDEEIVAKMVACTSEEELYSLFQDK"
     gene            6602..6892
                     /locus_tag="ACZPDN_01600"
     CDS             6602..6892
                     /locus_tag="ACZPDN_01600"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1297351.1"
                     /translation="MKVPKIQTVCGFGCGSSLMLRMNVEKIAKEHNYEIEAFCGDVGS
                     CCANQCDVIFISEELADRIKDRAQVPVVAIKNFMNKKEVEEKTLAYLEENCK"
     gene            6904..8178
                     /locus_tag="ACZPDN_01605"
     CDS             6904..8178
                     /locus_tag="ACZPDN_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIR1297352.1"
                     /translation="MGVLNFIINEIFNQGAIFLALIACIGLILQKKSFSEIVRGTLTT
                     AIGFYILSCGTGLITGNSIDGISAAFNAVTPSAVASKTVDIGAEFGTQIGIVMIIAFA
                     VNVLVARFTKWKSIFLTGHMLYWFPFVFIAAGVDAGLTGGKLIGLAAVFTAVYMIVSP
                     NLMRPLVKSVTGDDSFTIGHPTTCLSLLSAGLATLFGNKEHSTEDLNVPKSLGFLREI
                     SITGSITIAITYIVLYFILVANGKDPGTVWGYAGGTTGIFTYIFTHAIYFGVGITVML
                     QGVRMMIAEIVPAFKGIADKIVPNAIPALDVPVIFPYAPNALLIGFIVAMITSIITLL
                     LTSGMFPTVVIPLISTCFFETGCAAIIGNAKGGVRGCIIGAAACGIVMVFLVGFGAYF
                     FNNTIQQWMLVYGGQDFSLWGIVEGFIARLIA"
     gene            8180..8923
                     /locus_tag="ACZPDN_01610"
     CDS             8180..8923
                     /locus_tag="ACZPDN_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1297353.1"
                     /translation="MEFKYLERIHELMDVIEKDETKSMEECVDLLTTCILNKHSIYTF
                     GASHAGILSEELYYRAGGLMLFNPIFGREIMLDTSPITLTSKMERCVGYGTNLAQSKV
                     NFKKDDVLIVHSVSGRNPVAIEIAAEAKKMGAKVIAITNLSYSKSVTSRHPSKKKLYE
                     FADIILDNHGDVGDACVKIDGLEQKVSPTSTVIGAMMLNSIVAAVAQKLVDSGMKKPP
                     IFYSANLDGGDALNQKLYEEYKESIHYQF"
     gene            complement(9055..11577)
                     /locus_tag="ACZPDN_01615"
     CDS             complement(9055..11577)
                     /locus_tag="ACZPDN_01615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1297354.1"
                     /translation="MVEPTPSKDAFLVEHTDFSLREKIKYPNGGTSNFYDNGKDMVII
                     DKDICAHNSSDPVFSYMLITDKQNNYMHQFNRVFRFLGYSHLEVDKYLSKNEIVMPIN
                     PTRNVGDRSEQSDVSPLELHFEKSFSNAYGMNALKYITREYGICDENGKNYFLDYFVR
                     TKDEKYAVEENGVTYHHPQIIGEEKYKNQLRKQNTCASWGIKLFRFSTEDCAFENRIE
                     DDIKQYFGNDPSNFIDNGLKIDRSVELYEHQSISLQEIQKRRAAGIKAFLIVLPTAAG
                     KSKIVEEDLREFAKNKKDFKGLILVPGINILADWIQRIHNSLPELEDKIDIKTYAYMA
                     RHYTELSSDYYNYLVVDEAHHAVAPILKRVIQYYNTDFTIGLTATDQRPDKKKLETVF
                     GTYSTSLSLKEAMEKGIVAKANVYRIETNIDLSKVRFNGKDYVNADLEKRIRVTSRNK
                     LIVDVLKEYFTTGEASLRQGVIFCVNVSHANEMARLLNKAGIVASSYTGQTKNPESVM
                     KDFKNKKIRFLCTCNMISEGWDYPELGILVMARPTLSKVLYLQQIGRGLRKTDIKKNV
                     IVIDVVDEYGAMIKACNMHTIFANPYYVPFGDITITNYTPGDMLVVDGIEERIERITE
                     VDINTFEDKYGDYLSQEQLAREYFVNTGTVTSWIKKEKIKPSVEYKFGSKSLYLFSPD
                     DVEKYRNELGIKEHNNNTIKQDFFEFLEERDYSLSYKMPFLLAFIKNVNTIGDAKIDD
                     VLNDYIAFYKDRIDRGLQVDRSTCPYNETTLQDRKAICRNMLTNPFEKFERKRFLYYS
                     KDLSIIAMNHALYSKMNKEDWDRVKSQMQEDLEHYYSDMGGV"
     gene            complement(11541..12260)
                     /locus_tag="ACZPDN_01620"
     CDS             complement(11541..12260)
                     /locus_tag="ACZPDN_01620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1297355.1"
                     /translation="MRKKEYYFADFAEWSFEIDLNTTSRYYIHSIRLVFITTLGVFMT
                     SKTLDYYNKNSETFIQSTISADLKDMQNKFLNELNGNKILDFGCGSGRDTKCFIEAGY
                     DVDAIDGSIELCKSASAYTGTQVKHMLFQDLDKENYYDGIWACSSILHLPKSELKDVL
                     SKMNKALKDDGIIYTSFKYGDFEGERNGRFFTDFTLDIFKNFIKDVDNLMIKEYWITT
                     DVRPGREEEKWLNLLLQKMLS"
     gene            12374..12997
                     /locus_tag="ACZPDN_01625"
     CDS             12374..12997
                     /locus_tag="ACZPDN_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025380.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR1297356.1"
                     /translation="MKILLSILKGIKNGLSTAFRKVKSSKRLQGALVLVCIVAVACGA
                     LFAAYKKGTKVNETIQETVVNYKDIEEITFQEATVPEIGVIHKPMKLGKLEFDNPLTT
                     SDCIFTYNITVKAGYDLKDIKDDGGNELTQTITVTLPKATVYEPYVDEDSFTKYWDDG
                     KITANVGLDDIHAKKLEMIEHAKVVITDYIHSLKGYENYNVVFNEEA"
     gene            13002..13160
                     /locus_tag="ACZPDN_01630"
     CDS             13002..13160
                     /locus_tag="ACZPDN_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297357.1"
                     /translation="MNKVMVVKNFALRGFNDVRQNPQKYIFKLKKAKSVAIIACDAGE
                     RIISIMAR"
     gene            13747..14784
                     /locus_tag="ACZPDN_01635"
     CDS             13747..14784
                     /locus_tag="ACZPDN_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HIR1297358.1"
                     /translation="MRSANLLNDFAFKYVFGEDCKEANDALKSLLTVFLERSVNKVVV
                     KNSEMVKDYSKMKSPRLDLLVEFDDQTMVDLEMQLRQTKDNLMNRFPYYSARLHGSQD
                     MEGKSYSQLKETIVMIFFNVNIFENDNICNTFRYKCDGDLPFVKEEKEDRMKLRTIEM
                     PKVDLNKPLEDMNEQEKMIYYFLNCHKGMEDSKIKMMIESDEVIQMLEKRVETISDDR
                     WKKIIEDFQKLHENEERIELQLELEEAQKQIKEAQKAREEARKEKDAARKEKEVARKE
                     KDAARKEKEKANRQVEEANRHVENANKLVEQKNQEMKQIIQGMLSNGMSLEFVSKLLS
                     KSEDEIMELVK"
     gene            14861..15028
                     /locus_tag="ACZPDN_01640"
     CDS             14861..15028
                     /locus_tag="ACZPDN_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297359.1"
                     /translation="MKDQNCAYCMEGELVAAFGIKICELESSKVYLFKEQSHPGRVIV
                     AHKKHVSEITE"
     gene            15044..15310
                     /locus_tag="ACZPDN_01645"
                     /pseudo
     CDS             15044..15310
                     /locus_tag="ACZPDN_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013401.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            complement(15287..15730)
                     /locus_tag="ACZPDN_01650"
     CDS             complement(15287..15730)
                     /locus_tag="ACZPDN_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1297360.1"
                     /translation="MMYYTIGQVSQMFDLPISTLRYYDKEGFFPNLVRKGNIRYFSHN
                     ELEALHVIECLKKSGLGIKEINQFFMWVKQGSETYEKRKELFEARKSAVEAEIQNLQK
                     ALSLLEFKCWYYQKAIEDGTEENIQAMLPDKLPSDIQKLYDASHE"
     gene            15793..16335
                     /locus_tag="ACZPDN_01655"
     CDS             15793..16335
                     /locus_tag="ACZPDN_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1297361.1"
                     /translation="MSMKVLIISTSLRTNSNSDILARKCVRGALDTGHEVEYVSLKGK
                     NIEYCIGCLSCIKSGSCVLKDDVKDIMEKVKQAEVIIFATPIYYYEMCGQMKTLLDRL
                     NPLYASEYAFRDIYMIATAAEDEDSTFDKAYNGLQGWVDCFEKATLKGIVIGAGISEA
                     SDALNHKDVLKKAYELGRNL"
     gene            16545..17471
                     /locus_tag="ACZPDN_01660"
     CDS             16545..17471
                     /locus_tag="ACZPDN_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1297362.1"
                     /translation="MNFNRALSKEQTTCINGIFVMFVFLSHFGQYETMPWNNLLLAIG
                     QLMVAPFLFYSGYGIMEQIQRRGVAYIDGMPRKRILKFYIHFCMALCIYLFLSFLLGK
                     EYSFVRIVLSFTALSSIGNSNWYVFAILAMYSIVYISFKQCKKHSMTSCVLFTILYIV
                     MMDVIKDQAWWYNIILCFPAGMILSKYKDRVCSIIQKPVFFLFMITLALALYLFSFSI
                     LAYEIISIAFCFLIVDVCAFKEIKNDIFHFLGQYVFEIYILQRISMNIFDRYLNDWIY
                     LIVCILVTFVLAYNFKKLETKVDGLHIFKNFS"
     gene            17797..19923
                     /locus_tag="ACZPDN_01665"
     CDS             17797..19923
                     /locus_tag="ACZPDN_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1297363.1"
                     /translation="MKKILQASLSAVLVLSLVGCGSTKEEAVYQDSIDAYVNEIDMDY
                     AYDFTKTLSTDTSLHDNSLGFRTSGSDAEHRTANYLAKEMKAIGLKNVEKIPVNVDKW
                     QYNDASLTIEGTDIDLMPVSYMVNGTDENGITAQIVDCGTGFAKDYEGKDVEGKIALV
                     GVDQFNESWIGGYIYEAYEHGAKALVTYDLDGYGRFSDDDHQIQDVCAEDIMPTTIIT
                     MSEYKQIKKALKQGHDMATLKVDSVMDEGNGISYDVVGYIPGKSHDQQIIFAGHYDMY
                     FTGFQDDCSAIGTIMSMAKTMIDSGYVPENDIVVVAHGAEEWGATGTEFDWTRGAYEL
                     INNVHPEWANKTLALFNFELDAYDDGSDTFMVTCVPEYASLVKSLVDSGALDGAVKEY
                     KNGISTKTYDTTTMEDGVSYRNAGVPYFLNTTDTCSGETKDDGEYTWTQLHYHTESDN
                     TDTYSEKVMKANIAVFGSMAIAIDQLPAMSLNMQATIDDLKESFNENLALEAGVSKKD
                     WDKSLAVFEKEVDALNKEGQDINDRYVKAVNSKSDVTSIQKEGKAYNKKVLELFKYVQ
                     DNFVGIIFSSDIVMKHVGYQSNIEYMDEIIQDLKKDKVKDALDVAYQLNGLCEYNYYL
                     FSPNAAAKIDAHADKAVENNKWWGNDKGYYFTDTKNATTSLLNKEDGDDVSEEIKVYE
                     SARTKQLDSYKEVLENEILAMNKMGQ"
     gene            20076..21152
                     /locus_tag="ACZPDN_01670"
     CDS             20076..21152
                     /locus_tag="ACZPDN_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIR1297364.1"
                     /translation="MSESIPNHKTYKTIDEQIKYLKESKKIIVDKEDEHWFIDVNYIT
                     LINPYKDFLANGKDSNGNHIYPNYVNYKELLKIMNLELQFSDVLYKDIRNFERKFKNV
                     TFSILCKRYVQEQQDIYCLNYVNEIYNFNNQYSIEKNNKSILKYPMFCTRLFNTLTKS
                     GYIEKDYDSQSRVDLLQKIYCLGTGLKIDDCKVDTKNKLITHYVNRNRIAPLWTIPNA
                     LTLGEISMIFSMLDESIQNEIYCVMQNKTFFNKDSKFEYQKLSKYTGKIEYIRNMRNV
                     INHYEPIFPTLVATINNKAKLIKSSQLVSSLELLNDQWVYTVTNNQNLETNLNLNIPI
                     TTYNLPKIRLLELMETYIEKSHGK"
     gene            21303..>21498
                     /locus_tag="ACZPDN_01675"
     CDS             21303..>21498
                     /locus_tag="ACZPDN_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297365.1"
                     /translation="MCEYNYYLFSPNAAAKIDAHADKAVENNKRWDNDKGYYFTDTKN
                     ATTSLLNKEDGDDVSEEIKVY"
BASE COUNT         7316 a         2841 c         3997 g         7334 t
ORIGIN      
        1 aagtgaattc caacattgat taagcatcta gagaattctt ttaacatcaa ggattgtcca
       61 tatccagttg cataagcata gattcgattt gaagttgcca ttagtttata aatatcttca
      121 ataaatttat tttgttcaaa taaacgaatt gtagatgtca catcttctaa tacaacagaa
      181 gaaccaatct tattcgtgtt aataacgttt gcacgatcct ttcgatctag ttcgtttctt
      241 aaaaaatatc tgaattcact atagcctttg aatcctaatt tttgaacaat tcgaataata
      301 ctggatgcac tcgcattnnn nnnnnnncat ttgataactg tgctatttcg gatactgttt
      361 ttccttgaca taattccata ttttggaaaa taaaatttaa tatatataaa tcgttttcat
      421 ttaattgagt atgatgttga tttactaatt cttcaagtgt catatatatc taagtccttt
      481 acttcatata tctatcatat gtcaactttt ataaaaaata aaccttaatt tttttcttta
      541 aatgcatgtt cttacgtatt tatatcgttg gatgaaaatt caagaaatgg atggaaaaac
      601 agactttgat aaacaatttt aaagaccttt gatttattga attgaaattt attttaatgt
      661 agcatatgaa atgtaagagc ttgcaagtga tcattacaca ttttggaatt gtaaaggaga
      721 gaagattaca tgagaacttt tatacaaaag ctaggtaagt ccatgatggg accgttatct
      781 ataattgtag cagctggttt gttgttaggt atcactacta ttttaaccaa tgctagttta
      841 gttggagaag catttgcgag tgcagcaggt gttcaagttg ttgttggtgc agtatctgca
      901 ttagttggaa aggtatttgg attattacca gttttatttg cgatttctgt tgccactggt
      961 atggtaaaag aagataagga aattgcagga tttgcagttg ttatctgttt cgttttattc
     1021 cacgtaatgc ttaactacat gttaggatta aatggaatta cagctgagaa cacagctatt
     1081 gattatttaa caagtcatgg atcaagcaca ctagaggcat ataaaattaa tgcagcttat
     1141 gacacacaat taggaatttt cacttatcgt atgggtatct ttggaggtat cgttactggt
     1201 ttatggacag catttattca caataaattc catacacaaa aattacctgt agcatttagt
     1261 ttctttgctg gtaaccgttt tgttccaatt atgattatct taacagtgcc attcgtagca
     1321 attgtttcat actttgtatg gccattcttc agtatgctta ttgatggctt gggaactgtt
     1381 attagtaaat ctggtccatt tggattattc ttgtatggat ttgcagaacg tttattgatt
     1441 cctactggat tacaccacgt attgaaccaa ttgattcgtt tcactccagt tggtggaact
     1501 gcaatgatcg atggtacagt agtatctggt gcattaaata ttttccaagc agaattagtc
     1561 gcaaaaaata tggatatgaa cgttttccat caagcaactc gtttcttaac tcaaggaact
     1621 cacccattca tgatgtttgg tttaccaggc gcttgttacg caatgtataa gacagcttac
     1681 gcaaaagaac aacctaaagt tagaggtatg ttattagctt ctgcattaac ttcttttgca
     1741 actggaatca ctgaaccaat cgagttctca ttcatcttta tttcaccaat cttatgggta
     1801 ttccacgcat ttatggctgg tatgtctttc ttgattatgt ctttattaca agtcggtgtt
     1861 ggtaacgctg gtggtggttt cgtagacttg tttatcttcg gtatcctaca aggtacatat
     1921 actaaatggt acttctgtgt aatcctagga attgtttact tcttcgctta cttctttgta
     1981 ttcaaatatg taatcgaaaa gaagaactta aaaactcctg gacgtgatgt agaagatagt
     2041 agtgatgatg taggacaagt tgaagctagt gaattgggaa caacaatttt aaaagctatt
     2101 ggtggtaaag aaaacattcg cgacattgat aactgtattt caagattaag actaatttta
     2161 aatgatactt cagtagttga tgaagctgca ttaaaaacaa ctggatcatt aggaattatt
     2221 cgaattaatg aaaataatat tcaggttgta tatggtccaa aagtagaaga agcaacacac
     2281 gctttaaaac aagcagctgg tacgtataaa taataaccaa atatctataa gtgtattctt
     2341 atagatattt gtttagttaa ggagattaga tttatggaag taaaatatga aaatgtaaaa
     2401 ttacctgagt gcgattgcac aaatgttcca gtgcagttaa ctgatgaaac aatgcaggaa
     2461 cgattaaata aagtgctaga tagaatgaat cgtgaaaact ttgattcatt agtgatttat
     2521 gcagatttag aacacggtaa taattttgag tacttgacag gattcttacc tagatttgaa
     2581 gaagcattat tagttttgaa ttcaaatgga aatcattata tggttctagg aaatgaaaat
     2641 ttaaataaag cttcaaaagc gagaattaag aatacatcta ttcactgccc acacttttca
     2701 ttacctaatc aacctatgga tgttaatgaa ggaatcaaag atatcttaaa acgttgtgaa
     2761 tttaaaagcg gtgataaggt aggcattgtt ggatggaaaa acttcactag taagtatgag
     2821 gataatgctc aattattcga tgttccatat tatgttgtag atacaattaa agaacttgtt
     2881 ggaagtaatg tatttaatgc aacaagatta tttattggag aagatggtgc tcgttgcaca
     2941 aacaatgtaa atgaaattgt tcactatgaa tttggagctg cccttgcgag tgactgtatt
     3001 ttgaaggcta tgaatcactt ggatgtaggt gtttctgaaa tggaattggg aaacatctta
     3061 aatgcatatg gacaaagaaa tagtgtagtt acaattgcgg catctggacc tagatttatc
     3121 aaggcaaata tttatccaac aacgaacaca gtgaaattaa aagatccaat cagtttatcg
     3181 attggatata agggtggatt ctcatctaga gctggctatg cagtgcatga tagtagtgaa
     3241 ttggatgaag ctcttcaaga ttatttggat gtattagtaa aaccatattt ctcatccgta
     3301 gcagtatggt tagaaaaagt tcattgtgga atgcttggtg gcgaagttta tgatttgatt
     3361 gaagaaacaa tgccaaaagc tgaatacaat tggagtttgt gtcctgggca tttaacagct
     3421 gatgaagagt ggatgtcatc aaatatttat gagaatagta aagaattggt acaaagtggt
     3481 atgttgtatc aaattgatat tattccatca aaatcaggtt atgcaggtac aagtgcagaa
     3541 agtacagttt tattagcaga tgaaaatcta cgaaaacaga ttgcacaaga agaaccggaa
     3601 ttgtggaatc gaattcaaaa gagaagaaat tatataatta atgaattgaa tatttcatta
     3661 aatgatgatg ttttgccaat gtgttcaaca gtcgcgtact taaggccatt cttattaaat
     3721 aaagaaaagg cgatgaagtt atgagtcgca aattgaattc tttaaagagc tatatcataa
     3781 acgctttggc atttgtattt tcttattgta tgccggttgt aggattcttt attcttggct
     3841 atgcattgaa aaaggattta cctcaaacaa gaatctatgc gtcttgtgga gtccttcttg
     3901 catttttaac acaaatcgta acaagattag tttaaaatct tgttttacct aggaactcaa
     3961 atatgggttc ctttttttaa ttacagtttt ataacaattt acgcttgtgt aattttttgg
     4021 aaatagtgtg gtgtagtagg aaatgtagaa tgcagtgaaa cttaaacttg ccactttaaa
     4081 atttgttcaa taatgcagtt tgtgtgttaa taaaattgtt gtgaaaccgc aatcatttta
     4141 aattaattgt taaaaaaagt gccacattgc tggtttatat tttgtattga ggtaaaattt
     4201 taaaatgatt aaattataga tgaggtaaga aatatgattg cattaacttt aagacagaga
     4261 aacattattg aatatttatt aagccgcgat tcgtacgtga ctaatgatgt gattgcgtct
     4321 aattttgaag tgagcaatcg tacggtgcgt aaagacttag aagtaatttc tgcttactta
     4381 aaagaacaag gaatctcttt ggttcgaaag cctggtaagg gtgtatggat agagaataaa
     4441 caagaagtat tgtttgatga tttaagctgt tcttattcta agaatgagcg aattgttatt
     4501 tatgttacat cattgttatt gaatgatgtg aatacattta tgactcttgc ttctagttgt
     4561 ggtgtaagta aacaaacagt aattaatgat ttaaatacta ttgaaaaatt tcttgatgga
     4621 tatcatcttt cattaataaa aaatcagggt gttggtacaa gccttgaagg agatgagact
     4681 gatattcggc gtttgtttat tgatttgttg tttacaaatg atcattcgaa aattgtttat
     4741 gaactatgca taaataatga tggtattcaa agaaatctag atgtttcatt tgagctgata
     4801 acagtaattg aaaatgaatt tcgatttaga tatgtagata gagatcgaat ttccattgtt
     4861 ttaagctttt tgttgaatcg attgggttta atgaaaacaa aaaattcgac aattcaaagt
     4921 tcagacatgg aaagaatcaa ttctatcttg tctacgaaaa tttcggatag taatgatcaa
     4981 gaatatatta gttcgttctt attaggtgaa cgagtggagg ggtctttaac tttggattca
     5041 aaggttgaag atgaggcaaa tgaaataagt gaacatttga tttcatcttt aaagaagata
     5101 cattactttg attctgaaga tatgaaagat atcatacgca gtttaacagt acatgttcga
     5161 gcagcaattt atcgatgtag gaataacatt catattcgta atgaattgga tgagcaggtg
     5221 cgttttgcga ttggtgttat gtatgaattc acaaaaaaag aattgaatgt gtatgaaact
     5281 aaatacaatt taaattttga tcaaagtgaa atatcgtata ttgcattata tcttgcttct
     5341 atttttgaaa caggagtaca agaagattta aaagtagtta ttatgattgt ttgtactttt
     5401 gggttggcaa caagctctat tttaaaatct cgtatgtacc aaatttttgg agattgtttt
     5461 attgtaggac cttattcgat tgaagaggcg gagaaatatt tagagagcca tgaggtggat
     5521 ttgatcatat caacaaatta tatgagtgtg aaatcaacga atgtgattta tatcaatcca
     5581 ttattaaaac agaatgatat tgaaatgata agaaatacat tgtttcagga atcatttatg
     5641 aaaatgtgtt ctttgttctt taataagaat gagaataaag ttgtagaaca tgattatcat
     5701 tgtttaggtg agtatatttc gaaaaatgat gtttcaattt cgaaaggaaa aatatcttgg
     5761 gaagatgcaa tacgaaaagc agcagcacct ttagttgcta aaggagatat tgaaagtaag
     5821 tatgtaaata cgatgatcca ggcagtaaat gaatttggaa cttatatggt tattactcct
     5881 aaagttgctt atgtgcatgc tggcaaagat gatggagtga ataaaaattg tgtatcatta
     5941 ttgcttttag aaaatgaaat accttttggg tcatttaata caaagccagt tattgcgatt
     6001 gttgttttag gaatttgtga taaggaagaa tcacaattgt taaatattgc caatatattg
     6061 gatataccag aaaacataga gctattaaaa aatagtaaga atatagagtc gattttgagt
     6121 ttacatgatt gaggagaaaa tagagatgag tacaggaata aaattacaaa atattcaaat
     6181 tggtttagaa gcaaaggatt ggaaagaagc aatccgtata gctagtgtgc ctttagttaa
     6241 atgtggttca attgaagaac cttatgttga aaatatgata agtagtgtag aaacattagg
     6301 accatatata gtgattatgc cccaatttgc gttggctcat gctgctccgt gtgagtatgt
     6361 aaaaaaatca gatatttcaa tcgcaacgtt taaaaatgat attgaatttc attgtgataa
     6421 cgatcccgta agaattgtaa tgtgtttggc ttgtacagat aaaacaagtc atatagaaat
     6481 gttacaaagt gttgccacta cactaatgga tgaagagata gtggcaaaga tggttgcctg
     6541 cacaagcgaa gaagaactat attctttgtt tcaggataaa tagagaaagg agtgagaaag
     6601 aatgaaagta ccaaagattc aaactgtttg tggatttgga tgcggctcta gcttgatgct
     6661 tcgtatgaat gtagaaaaaa tcgcaaaaga acataattat gaaattgaag cattttgtgg
     6721 agatgttggt tcatgttgtg caaaccagtg tgatgtaatt tttatttctg aggaattggc
     6781 tgatcgaatc aaagatcgtg cacaggttcc tgttgtggct attaaaaatt tcatgaacaa
     6841 aaaagaagta gaagaaaaaa ctttagctta cttagaagaa aattgtaaat aaggagagta
     6901 tgtatgggag tattaaattt tattattaat gaaatcttca atcaaggcgc tatattcctt
     6961 gccttgattg cttgtattgg tttgattctt cagaaaaaat cattttctga aattgtacgt
     7021 ggaacattaa ctacggcaat cggtttctat attctatctt gtggtactgg tttaattact
     7081 ggtaattcta ttgatggaat ttcggctgct tttaatgcag taacaccaag tgcagttgca
     7141 agtaaaacag tggatattgg agctgaattt ggaacacaga ttggtattgt aatgatcatt
     7201 gcgtttgctg ttaacgtatt agtggctcgt ttcacaaaat ggaaatctat tttcttaact
     7261 ggacatatgt tatattggtt cccatttgta tttattgctg caggtgtaga tgctggatta
     7321 actggtggta agttaattgg attagcagct gtatttactg cagtatatat gattgtttct
     7381 ccaaatttga tgagaccatt ggtaaaatct gttactggtg atgattcatt cacaattgga
     7441 cacccaacaa catgtttatc attattatct gcaggattag caactttatt tggaaataaa
     7501 gaacattcta cagaagactt aaatgtacct aaatcattag gattcttaag agaaatctca
     7561 attactggat caattactat tgcgattact tacattgttt tatattttat cttagtagct
     7621 aacggaaaag atcctggaac tgtttgggga tatgcaggtg gaactactgg tattttcaca
     7681 tatatcttta cacacgctat ttactttggt gttggtatta ctgtaatgct tcaaggtgtt
     7741 cgtatgatga ttgctgaaat cgttcctgca tttaaaggta ttgcggataa gatcgttcca
     7801 aatgctattc cagcattaga tgttccagtt attttccctt atgcaccaaa tgctttatta
     7861 attggattta tcgttgctat gattacatca attattactt tattactaac tagtggaatg
     7921 ttcccaacag ttgtaattcc attgatttca acttgtttct ttgaaactgg atgtgctgct
     7981 attattggta atgccaaagg tggagttcgt ggatgtatta ttggagccgc tgcatgtggt
     8041 atcgtaatgg tattcttagt tggatttgga gcttacttct tcaataacac aattcaacaa
     8101 tggatgttag tttatggtgg tcaggacttc tctttatggg gaattgtaga aggatttatt
     8161 gctcgattaa ttgcttagta tggaattcaa atatttagaa cgcattcatg agttaatgga
     8221 tgtcatcgaa aaagatgaaa caaagtccat ggaagaatgt gttgatttac tgaccacatg
     8281 tatcttaaat aaacattcta tatatacttt tggtgcaagt catgcgggta ttttgagtga
     8341 agaattgtat tatcgtgctg gaggtttgat gttatttaat ccaatctttg gtagagagat
     8401 tatgcttgat acatctccaa ttactttaac aagcaaaatg gaaagatgtg ttggttatgg
     8461 aacaaatcta gcacaaagta aagttaactt taaaaaagac gatgtattaa ttgttcattc
     8521 tgtttctgga agaaatccag tagccattga aattgcagca gaagcgaaaa aaatgggcgc
     8581 caaagtaata gctatcacaa atttatctta ttctaaaagt gtgacttctc gtcatccatc
     8641 aaaaaagaaa ttgtatgaat ttgctgatat tattttggat aatcatggtg atgttggtga
     8701 tgcttgcgta aaaattgatg gtttagaaca aaaggtttct ccaacttcaa ctgtaattgg
     8761 agcaatgatg cttaattcga ttgttgctgc tgttgcacaa aaattagtgg attcaggcat
     8821 gaaaaaaccg ccaatctttt atagcgctaa cttagatggc ggcgatgcat tgaatcaaaa
     8881 attgtatgaa gagtataagg aaagtattca ttatcaattt tgattcagca gaacaattat
     8941 agcataacaa acaggagctg taacggataa ggaagtttca gctcctttgt aaagatatgt
     9001 gccctatttg gcgcttacaa aaaaacggtt tggctgttta tggtcaaacc ttttctatac
     9061 tccacccata tcagaataat agtgttctaa atcttcttgc atttgtgatt taactctatc
     9121 ccaatcttct ttattcatct tactatagag tgcatgattc attgcgataa tgcttaaatc
     9181 ttttgaataa tataagaatc tttttctttc aaatttttca aatggatttg tcagcatgtt
     9241 tctacagatt gcttttctat cttgtagagt ggtttcgtta tatggacaag tgctgcgatc
     9301 tacttgaagt cctctatcaa ttcgatcttt gtagaatgca atataatcat ttaatacatc
     9361 gtctattttt gcatcgccaa tagtattaac atttttaata aaagctaata agaatggcat
     9421 tttataagat aatgaataat ctctttcttc taagaattca aagaagtctt gtttgattgt
     9481 attgttgtta tgttctttga ttccaagttc atttcgatat ttttcaacat catcaggact
     9541 aaataagtat aaggatttgc taccaaattt atattctaca ctaggtttta ttttttcttt
     9601 tttaatccag cttgtaacag ttccggtatt gacaaaatat tctctagcta attgttcctg
     9661 acttagataa tcaccatact tatcttcgaa tgtattaata tctacttcag tgatacgttc
     9721 aatacgttct tcaataccat caacaactaa catgtctcca ggagtgtaat ttgttattgt
     9781 gatatcacca aatggtacat aatatggatt ggcaaatatt gtatgcatat tgcaagcttt
     9841 gatcatagcc ccatattcat caacaacatc aatcacaatt acatttttct taatgtctgt
     9901 ctttcgtaaa cctctaccga tttgttgtaa atacagaact ttagaaagag taggtctagc
     9961 cataactaaa atgccaagtt caggataatc ccatccttca gaaatcatgt tacaagtaca
    10021 aagaaaccga atcttcttgt ttttgaaatc tttcatgaca gattctggat ttttggtctg
    10081 tccagtatag ctagatgcaa caatgccagc tttatttaaa agtcgtgcca tttcatttgc
    10141 gtgagaaaca ttaacacaga atataacacc ttggcgcaaa cttgcttctc cagtagtaaa
    10201 gtattctttt agtacatcta caattaattt gtttcgagaa gttactctga ttcttttttc
    10261 taaatctgca ttcacatagt ctttaccatt aaaacgcact ttagataaat caatatttgt
    10321 ttcaattcga tatacatttg ccttggcaac tatacctttt tccatagctt cttttaaaga
    10381 caatgaagtg gaatatgtac caaatacagt ttctaatttt ttcttatccg gtctttgatc
    10441 agttgctgtc aaaccaattg tgaaatccgt attataatat tgaatgactc tttttagtat
    10501 gggagccacc gcatggtgag cttcatcaac aactaaatag ttataataat cagaagataa
    10561 ttctgtataa tgtcttgcca tatatgcata ggtttttata tcaattttat cttctagttc
    10621 aggtaaagaa ttatgaattc tttggatcca gtctgcaaga atattaattc ctggaactag
    10681 tattaatcct ttaaaatctt ttttattctt ggcaaactca cgcaaatctt cttcaacaat
    10741 ttttgattta ccagccgctg ttggtaatac aattaaaaat gctttaattc cagcagccct
    10801 tcttttttgg atttcttgta aactgataga ttggtgttca tataattcaa cagatctgtc
    10861 tatctttaaa ccattatcaa taaaattaga tggatcattt ccaaaatatt gtttaatatc
    10921 atcttcaata cgattttcaa atgcacaatc ctccgttgaa aaacggaata atttgattcc
    10981 ccatgaagca catgtatttt gttttcttaa ttggttttta tatttttctt cacctataat
    11041 ttgaggatga tgatacgtta caccattttc ttcaactgca tatttttcat cttttgttcg
    11101 cacaaaataa tcaagaaaat aattcttccc attttcatcg caaataccat attctcttgt
    11161 tatatattta agtgcattca tgccatacgc atttgaaaaa ctcttttcga aatgtaattc
    11221 aagtggagaa acatcagatt gctcacttct gtctccaaca tttcttgtag ggtttatagg
    11281 cattacaatt tcattttttg aaagatattt atcaacttct aaatgagagt agcctaaaaa
    11341 acggaagact ctgttgaatt gatgcatata gttattttgt ttatctgtaa taagcatata
    11401 tgaaaataca ggatcacttg aattatgtgc acaaatatct ttatcaataa taaccatgtc
    11461 ttttccgtta tcatagaaat tagatgttcc accatttgga tatttgattt tttctcttaa
    11521 agaaaaatca gtatgttcaa ctaagaaagc atcttttgaa ggagtaggtt caaccatttt
    11581 tcttcctccc ttcctggtct tacatctgtt gtgatccaat attctttaat cattagatta
    11641 tctacatctt taatgaagtt tttaaaaata tccaaagtga aatctgtaaa gaatcttccg
    11701 tttctttctc cttcgaaatc accatattta aatgatgtat aaataattcc atcatctttt
    11761 aaagctttat tcatttttga taagacatct tttagttcag actttggtaa atgtagtata
    11821 gaagaacatg cccaaattcc atcataataa ttttctttat ctagatcttg aaacaacatg
    11881 tgttttacct gagtacctgt atatgcactg gcacttttac ataattctat agatccatca
    11941 atcgcatcca cgtcatagcc agcttcaata aaacattttg tatctctacc agatccacat
    12001 ccaaaatcta gtattttatt tccgtttagt tcatttaaaa atttattttg catgtccttt
    12061 aaatcggcag atattgtact ttgaatgaat gtttctgagt ttttattata ataatccaat
    12121 gttttgcttg tcataaatac ccctaatgta gttataaata ctaatctaat actgtgtata
    12181 taatatcttg aagttgtatt caaatcaatt tcaaatgacc attccgcaaa atctgcgaaa
    12241 taatactctt ttttgcgcac tcttttatct gtagaaaatg cttatatcga attatgataa
    12301 agatgcttaa agatatcaag aagcaagaaa taaataaatt gaaacataga tttcaaaaag
    12361 gagaaaaaag agtatgaaaa tattattaag cattttaaaa ggaattaaaa acggactttc
    12421 tacagcattt agaaaagtta aatcgagcaa gagattgcaa ggagctttag ttctagtttg
    12481 tattgtggca gttgcatgtg gtgctttgtt tgcagcttac aaaaagggta caaaagtaaa
    12541 tgaaacgatt caagaaactg ttgtaaatta taaggatatt gaagaaatca catttcaaga
    12601 agcaacagtt cctgagattg gagttattca taaaccaatg aaattaggaa aacttgaatt
    12661 tgataatcct ctaacaacta gtgattgtat ctttacatat aacattactg ttaaggctgg
    12721 atatgattta aaagatatca aggatgatgg tggtaatgaa ttaactcaga ctattaccgt
    12781 aactttacct aaggctacag tttatgaacc atatgttgat gaagattcat ttactaaata
    12841 ttgggatgat ggaaagatca ctgccaatgt cggattagat gatattcatg ctaagaaact
    12901 cgaaatgatt gaacatgcta aggtagttat tactgattat attcactcgc ttaagggtta
    12961 tgaaaactac aatgtagtat ttaatgagga ggcgtaatac tatgaacaag gtaatggttg
    13021 ttaagaattt tgctttacga ggatttaatg atgtgagaca aaatcctcaa aagtatatat
    13081 tcaagcttaa gaaggctaaa tcagttgcta ttatcgcatg tgatgcgggt gaacgtatta
    13141 ttagcataat ggctcgttga taaattgaat gataaggatt aatgccttgt cattcatcta
    13201 agtataaaag gtttatattt agatgaatga ttctagttga gaaaatagag atctaactaa
    13261 aacctccaac atataaccta tttccatagt tggatttcaa atttcttaac tttgcataga
    13321 gtgcttaagg gcactcaaat aaaaagactt cagaatagta atctggagtc ttttgggtta
    13381 tatgggtaaa aaaaattaga gctggatttg agtgtgtttg tgaaatggta ccagctctag
    13441 aaaatggatg ttgattagtt ggttaatcaa cactcatagt ttaacatgaa ttatttttgt
    13501 tgtatataat tatttttatt aaatggatac gctttcatca cttagttgac ttttgtttgc
    13561 ggcgagaaaa tatttaaaaa tgaaacgatt gcgagaattt tttcatcatc tatcaacgtt
    13621 tttttttttt tgtatttaca tgcaaataag aaaattttgc ttgttttttt ttaacttttc
    13681 taaaaattgt ttaataaatt tcgtatatga gtatgtagga aaacacctac tagaaaagag
    13741 gtgcttatgc gtagcgctaa tttattaaat gatttcgcct tcaagtatgt gtttggcgaa
    13801 gattgtaaag aggccaatga tgccttaaaa tcattactta cggtattttt agaaaggagt
    13861 gtgaataaag ttgttgtaaa gaattcagaa atggtaaaag actatagtaa aatgaaaagt
    13921 ccacgtttgg atctattagt tgaatttgat gatcaaacga tggtagattt agaaatgcag
    13981 ttaagacaaa ccaaagataa tttaatgaat cgctttcctt attattcggc tagacttcat
    14041 ggctcacaag atatggaggg gaaaagctac agtcaactaa aggaaaccat tgttatgatt
    14101 ttctttaatg tgaatatttt tgaaaacgat aacatatgta atacattcag gtacaaatgt
    14161 gatggtgact taccttttgt gaaagaagaa aaagaagatc gtatgaagtt acgtacaatt
    14221 gaaatgccaa aagtagattt aaataagccg ctagaagaca tgaatgaaca ggaaaagatg
    14281 atttattact ttttgaattg ccataaagga atggaagata gtaagattaa gatgatgata
    14341 gaaagtgatg aggtgattca gatgttagag aagcgtgtag aaactatatc ggatgatcgt
    14401 tggaaaaaaa taatagaaga ttttcaaaaa ctgcatgaaa atgaagagcg aatcgaacta
    14461 cagcttgaac ttgaagaggc tcaaaaacaa ataaaagagg cccaaaaagc acgagaagaa
    14521 gcacgtaaag aaaaagatgc agctcgcaaa gaaaaagaag tagctcgtaa agaaaaagat
    14581 gcagctcgta aagaaaaaga aaaagctaat agacaagtag aagaagctaa tagacatgtt
    14641 gagaatgcaa ataaactagt agaacaaaag aatcaagaaa tgaaacagat aattcaaggg
    14701 atgctttcga atggtatgag tttagaattt gtttcaaagt tgttgtctaa gagtgaagat
    14761 gaaattatgg agttagtgaa atagctaaag ataaatgatg atataaagca ggtttttgag
    14821 gcctgctttt ttttaaaaaa aggaagagta ggtagataaa atgaaagatc aaaattgtgc
    14881 ttattgcatg gaaggcgagc ttgttgctgc tttcggaatc aaaatttgtg aattagaaag
    14941 ttcaaaggtg tatttgttta aggaacaaag tcatccgggt cgtgtgattg ttgcccacaa
    15001 aaaacatgtt agtgaaatca cagaatgaac accaaaagaa agagctgctt atatggagga
    15061 tatcaataag gtggcttgtg ctatgcatga aatcttcaat ccagataaag taaattatgg
    15121 agcatatggg gatacggaac atcatcttca tttccattta gtaccaaaat acaaagatgg
    15181 atatgaatgg ggtggtgtat ttgctatgaa tcctggtgtt aagactttgt ctgaagaagc
    15241 ttatcaggaa atggtagaaa aatatcgcga agtattagct gaatatttat tcgtgactgg
    15301 catcgtataa tttttggata tcactaggaa gcttgtctgg aagcatggct tgaatgtttt
    15361 cttcagtccc atcttctata gctttttggt agtaccaaca tttgaattct aaaagggaaa
    15421 gtgctttttg aagattttga atttcagctt ctacagctga ttttctagct tcaaataatt
    15481 ctttcctttt ttcataagtt tcactacctt gtttgaccca cataaagaat tgattgattt
    15541 cttttattcc aagacctgat ttttttaagc attcaatgac gtgtaaggct tctaattcat
    15601 tgtgactaaa gtagcgaata tttcctttgc gcacaagatt aggaaagaat ccttctttat
    15661 cataatatct aagtgtagaa atgggtaaat caaacatttg tgaaacttgt cctattgtgt
    15721 aatacatcat ttgtcctcgc tttcttgacc taaagtcaac ttcaggtagt atagtaatac
    15781 caggaggatt gtatgtcaat gaaggtttta attatatcaa caagtctaag aacaaatagt
    15841 aattcggata ttttagctag aaaatgtgtt agaggtgcat tggatacagg acatgaagta
    15901 gagtatgtgt cattaaaagg aaaaaacatt gagtattgta ttggatgttt atcttgtatc
    15961 aagagtggta gttgtgtatt aaaggatgat gttaaagaca ttatggagaa ggtaaaacag
    16021 gcagaagtca ttatatttgc gactcctata tattattatg aaatgtgtgg tcaaatgaaa
    16081 acattactag atcgtttgaa tcctttatat gcaagtgaat atgcatttag agatatctac
    16141 atgatcgcaa cggctgcaga agatgaggat agtacatttg ataaagctta taatggactt
    16201 caaggctggg ttgattgttt tgaaaaggct actttaaaag ggattgtgat aggtgctggt
    16261 ataagtgaag caagtgatgc attaaatcat aaggatgttt tgaagaaggc ttatgagttg
    16321 ggtaggaact tgtaatgttt tgttaaaatg atgattttac ggatgaaagc tggatatcca
    16381 cctattgata ttaaatttgc agattgaatt gttcattata atgcgtttca tgaaagtcat
    16441 ataaaacata gtcttttggc attggaaaat aggttacaag attaagtgga ctcaaatgtc
    16501 cactttgttt ttgtgttata atactttctt gtagagggca taaaatgaat tttaataggg
    16561 cattaagtaa agaacaaaca acctgcatca atggaatttt tgtgatgttt gtgtttctat
    16621 ctcattttgg acaatatgaa acgatgccat ggaataatct cttgttggca attggacaat
    16681 tgatggtagc accattcttg ttttattcgg gatatggcat tatggaacaa attcaaagaa
    16741 gaggtgttgc gtatattgac ggtatgccta gaaaaagaat attgaagttt tatattcact
    16801 tttgtatggc attatgtatt tacttgtttt tatcattctt attaggaaaa gaatattcct
    16861 ttgttcgtat tgtattatca tttacggctt taagttccat tggaaatagt aattggtatg
    16921 tgtttgcgat attagcaatg tatagtattg tatatatttc gtttaagcaa tgtaagaaac
    16981 atagcatgac ttcatgcgta ctatttacca ttctttatat agttatgatg gatgtaataa
    17041 aggatcaagc ttggtggtac aatatcattc tttgttttcc tgcaggtatg attctatcga
    17101 aatataaaga tcgggtttgt tctatcattc aaaagccagt attcttttta tttatgatta
    17161 ctttagcttt ggctttgtat ttattttctt tttctatact cgcatatgaa ataatttcga
    17221 ttgcgttttg tttccttatt gtagatgtat gtgcatttaa agaaataaag aatgatatat
    17281 tccatttttt aggtcaatat gtatttgaaa tctatattct tcaaagaatt tcaatgaata
    17341 tatttgatcg atatttaaat gattggatct atctaattgt atgtatactt gtcactttcg
    17401 ttcttgccta taattttaaa aagctagaga caaaagtgga tgggttacat atttttaaaa
    17461 attttagttg acaataaata tcattttcct gtatattttt ttgcgtacaa atcgtacaga
    17521 aattgaccgc ctagatatag gctaaggcta cacggacggc cgggtcaaat gccgaagact
    17581 tgttgaagaa gcaagaaccg tatttaggga atacggaagg gtgtttaccc ttattgattg
    17641 agttaaaagc tcagttttgt aaagtacaat ataccttatg aaatggtcaa taagctggaa
    17701 tttctgttac gaaaagtatg cagatagatg atggacgaag ataggtgtat attgtgcgcc
    17761 tatctttttt tgtgtttggt aaaggagaaa aggaatatga aaaagatttt acaagcaagc
    17821 cttagtgcag tattggtgtt gagcctagtg ggttgtggtt cgacaaaaga ggaagcagtt
    17881 tatcaagatt ctattgatgc ctatgtgaat gaaattgata tggactatgc gtatgacttc
    17941 acaaaaacat tgtctacaga tacttcatta catgataatt cacttggatt tagaacgagt
    18001 ggttccgatg cagagcatag aaccgcaaat tatttggcta aggaaatgaa ggccattggt
    18061 ttaaagaatg tagaaaagat acctgtgaat gtggataagt ggcagtataa tgatgcttca
    18121 cttacaattg aaggtacaga cattgatttg atgcctgtat cttacatggt gaatggtaca
    18181 gatgaaaatg ggattacagc acaaatcgta gattgtggta ctggttttgc gaaagactat
    18241 gaaggcaaag atgttgaagg taagattgca ttagttggcg tggatcaatt taatgagtct
    18301 tggattggtg gatatatcta tgaggcgtat gagcatggtg caaaagcatt agtcacatat
    18361 gatttagatg gatatggaag atttagtgat gatgatcatc agattcagga tgtttgtgca
    18421 gaagatatta tgccaacgac aatcatcaca atgagtgagt acaaacaaat taaaaaggct
    18481 ttaaaacaag gccatgatat ggccacttta aaagtagata gtgtgatgga cgaaggcaat
    18541 ggtataagtt atgatgtggt tggctatatt cctggtaagt cacatgatca acaaattatt
    18601 tttgccggac attatgatat gtattttact ggttttcaag atgattgtag tgctattgga
    18661 accattatgt cgatggctaa gacaatgatc gatagtggct atgtgcctga aaatgatatt
    18721 gtggttgttg cgcatggtgc tgaagaatgg ggcgcaacag gcactgaatt tgactggaca
    18781 agaggtgctt atgaattgat caataatgtg catcctgaat gggcaaataa aactttagct
    18841 ttatttaact ttgaattgga tgcctatgat gatggttcag atacattcat ggtgacttgt
    18901 gttcctgaat atgcatcttt agtaaaaagc ttagtggatt ccggtgcatt ggatggtgct
    18961 gtaaaagaat ataagaatgg tatttctaca aaaacatatg atacaacgac aatggaagat
    19021 ggtgtttctt atcgtaatgc aggagttcca tatttcttaa atacaacaga tacttgttct
    19081 ggagaaacaa aggatgatgg tgaatatact tggacacaac ttcattatca tacagaatcc
    19141 gataatacgg atacgtatag tgaaaaggtg atgaaggcta atattgcggt atttggaagt
    19201 atggctatcg ccatcgatca gcttccagct atgtctttaa atatgcaggc taccattgat
    19261 gatttaaagg aaagctttaa tgaaaattta gctttggaag ctggtgtttc taaaaaggat
    19321 tgggacaagt ctttagctgt ttttgaaaaa gaagtggatg cactaaataa agagggtcaa
    19381 gatattaatg atcgttatgt aaaggccgta aactctaaat cggatgttac ttcgattcaa
    19441 aaagaaggaa aagcctacaa taagaaagta ttagaattat ttaaatacgt acaggataat
    19501 tttgtcggaa ttatattctc gagtgatatt gtgatgaaac acgtgggata tcaaagcaac
    19561 attgaatata tggatgaaat cattcaagat ttaaagaaag acaaagtaaa ggatgcccta
    19621 gatgtggcat accaattaaa tggcttgtgt gaatacaatt attacttatt ctcaccaaat
    19681 gcggctgcta aaattgatgc gcatgcagat aaggcagttg aaaacaataa gtggtggggc
    19741 aatgataaag gctactattt cacagacaca aaaaatgcta caacttcttt attaaataaa
    19801 gaagatggag atgatgtaag tgaagaaatc aaagtatatg aaagtgctag aacgaaacag
    19861 ttagattctt ataaagaagt gcttgaaaat gaaatcttgg caatgaacaa gatgggtcaa
    19921 taaatataga cttggcaata taaatgccag gcttttcttg tttataaaag aaggaatagt
    19981 agaactcata accctttaat aacatttatt atgcttagta ttataggtgc tttcatgtta
    20041 aaatagatat aataatataa gaaagtgtga attaaatgag tgaatcaatc ccaaatcaca
    20101 aaacatataa gactattgac gaacagataa aatatttaaa agaaagtaaa aaaattatcg
    20161 ttgataaaga agatgaacat tggtttattg acgtgaatta tataacatta ataaaccctt
    20221 ataaagattt tcttgcaaat ggtaaagatt caaatggaaa tcatatatat ccaaattatg
    20281 taaattataa ggaactttta aagataatga atttagaatt acaattctct gatgttttat
    20341 ataaagatat ccgtaatttt gaaagaaaat ttaagaacgt aacattttca attctatgca
    20401 aaagatatgt gcaagaacaa caagatattt attgtcttaa ttatgttaac gaaatctata
    20461 attttaataa tcaatatagt attgagaaaa ataataaaag tattttaaaa tatcctatgt
    20521 tttgcactcg tttatttaat actttaacaa agtcaggata tattgaaaaa gactatgatt
    20581 ctcaatcgcg tgtggatctg ctgcagaaaa tttattgttt gggtactggt ttaaaaattg
    20641 atgattgtaa ggtagacaca aagaataaat taataacaca ttatgtgaat cgtaatagaa
    20701 tcgcccctct atggacaata cctaatgcgt taacacttgg tgaaatatct atgatttttt
    20761 ctatgcttga tgaatctata caaaatgaga tttattgtgt catgcaaaat aaaactttct
    20821 ttaataaaga ttctaaattc gaatatcaaa aattaagtaa gtatacagga aaaatagaat
    20881 atattcgaaa tatgcgtaat gtaattaatc attatgaacc aatattccca acgttagtag
    20941 ctacaattaa caataaagcc aaattaataa aaagttccca gttagtttca tctttagaac
    21001 tacttaatga tcaatgggtg tatactgtaa caaataatca aaatttagaa accaatttaa
    21061 atttaaatat tccaataacc acgtacaatt taccaaaaat tagattgtta gaattaatgg
    21121 aaacatatat tgagaaaagc catggtaaat agatactatg gcttaaaaca ataaaaaaat
    21181 ccggcctcca tctggttctt gtccaggtag gattccggtt tgcgcatctc gcgccttcgt
    21241 catatgtaat ataccatatt tttttaaata atacaaaaaa atgaatttat aaattaaatg
    21301 gtttgtgtga atacaattat tacttattct caccaaatgc ggctgctaaa attgatgcgc
    21361 atgcagataa ggcagttgaa aacaataagc ggtgggacaa tgataaaggc tactatttca
    21421 cagacacaaa aaatgctaca acttctttat taaataaaga agatggagat gatgtaagtg
    21481 aagaaatcaa agtatatg
//
LOCUS       DBMDWL010000013        21176 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_896_length_21176_cov_2.968493, whole genome shotgun sequence.
ACCESSION   DBMDWL010000013 DBMDWL010000000
VERSION     DBMDWL010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 21176)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21176)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21176
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_896_length_21176_cov_2.968493"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="ACZPDN_01680"
     CDS             <1..291
                     /locus_tag="ACZPDN_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HIR1297366.1"
                     /translation="AFEFYNERNVEDCIQIPWDTIDRVEASVLFNKSISRFVIFINEK
                     TYFTFSTKDNKKTLRAIREYVPADRMLRSLSFWDVFKRGLMYIPNKLLHSNN"
     gene            638..1954
                     /locus_tag="ACZPDN_01685"
     CDS             638..1954
                     /locus_tag="ACZPDN_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297367.1"
                     /translation="MLYRKVERTIEQHLKNGSNKILLVDGARQIGKTYIIRYVGNKLF
                     ENYIEINMIEDSLGNRSFEKVRTVEDFYLQVSMLAGAKMQEKENTLIFIDEIQAYPHL
                     LTLLKFLSQDNRFTYIASGSLLGVTLSQTTSIPMGSIQKIQMYPLDFEEFLYANGMNE
                     FVVSTLRNKFNHLEELDESMHNKMMDLFKKYLLVGGMPDAINTYLSEKNIVSVRNIQA
                     EIHEYYASDASKYSLENKLKISRIYDLIPSNMENKKKRVVVKNIEGKRGKTFNDYQEE
                     FEYLVSAGIALNVQAISNPKFPLIESASKNLMKLYLNDVGILTGILYGNNINAILDDQ
                     KSINLGSVYETIVASELKAHGKKLYYYDNRNKGEVDFLIDDYDSLSVVPIEVKSGKDY
                     TVHSALNNFVNNNDYHIQKGYVVSNERTIRQKGKIIYLPIYYIMFF"
     gene            complement(1971..2822)
                     /locus_tag="ACZPDN_01690"
     CDS             complement(1971..2822)
                     /locus_tag="ACZPDN_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR1297368.1"
                     /translation="MINKIKVLAADVDMTLSSKSSPLPEITIQALQELHNRGVKLGLA
                     TGREITEMEKTKGIFWKLGFEFDFIIGMNGGMIYDRNLDKTFTMDMMSIEEMKEVLTF
                     LMPIIEENKVSINVEGNQLRNVMNISEDLLDMSKRRNIPLIDKTGDIDGFCEKPVYKF
                     LFRTDPETTQLIRDQVEEQYKGKYQTIETFPGTIELMYSGFSKGRGLEMYCERNQIHL
                     ENTIAFGDNENDNSLLLTSGWGVCLKDGNPNTKSLADDITDYDCLEGGVGHYIFDHIL
                     NNKDVEF"
     gene            2959..4119
                     /locus_tag="ACZPDN_01695"
     CDS             2959..4119
                     /locus_tag="ACZPDN_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1297369.1"
                     /translation="MNFLVRRFIKNYQDTKDANVRTNIGKLSGIVGIFSNLFLFVIKF
                     VIGTIVHSVSIQADGVNNLTDAGSNIISILSFHLANKPADKDHPFGHERTETIASLFV
                     GILILVLGFETAKDSISKVIHPGSIDFRIASVIILLISIIVKFWMYAYNKKLSKTYDS
                     SLLEATALDSISDVCGTTAVLVSTLLSPVLHFNLDGYMGIVVSGIILYGAYGLLRDMI
                     NSLIGEAPDPELVHNIVDRIMAHPAILGVHDMMLHNYGPNKIFASAHVEVDSSKDIFE
                     THDHIDNIEREVKENMNIDLVLHMDPVKVNDPETELYRAKVVEAIHQIDPKWGFHDFR
                     IVSGPTHVNLVFDLVIPFEEKYSQEEIEDMLLKHIQSDKKIYLVLTIDHPYA"
     gene            complement(4131..4889)
                     /locus_tag="ACZPDN_01700"
     CDS             complement(4131..4889)
                     /locus_tag="ACZPDN_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1297370.1"
                     /translation="MSKKKDERQFKIRQLLQEHPKIKISDLADYFQTSSETIRKDIIE
                     LEDSKIIKKERGYALLLEEPDEMSISLRNQEYIEEKKQIMKKACTFIKEGMVVYLDAG
                     STCLAGIPFLQSIHNITIVTNSIFVAYKCAKLNMHVILIGGNISNNAYRSYGNFASET
                     IDYIHINVAILGTKGFKDNDGFTTYENEYELKRHILQQSEKIIVVTDKNKFNAQPEYT
                     YCKFKEVDVFISNTLTKENKSQIQVIPEIIEAEV"
     gene            complement(4893..5660)
                     /locus_tag="ACZPDN_01705"
     CDS             complement(4893..5660)
                     /locus_tag="ACZPDN_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1297371.1"
                     /translation="MSKKKEERQLQIRQILEGKEKIKVAELAKLLSVTPETLRKDLDE
                     LQERKILIREHGYAKIQSSSVETFVELKAQKNKEMKKIVALEAFSRIENGMIVYLDSG
                     STILTAISALRNKSDITIVTNSILVAYECANMNLNIIMAGGLLFNIGKRTYGHFATEI
                     IDHLSIDLAIMGTDAFTEKSHGFTTISADELGFKRHVMNQSAKKIMISDASKVNNKAD
                     IAPYAFCKFNEFDEFITNHLTAEQYDVVKTVKKVTQV"
     gene            5817..6074
                     /locus_tag="ACZPDN_01710"
     CDS             5817..6074
                     /locus_tag="ACZPDN_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297372.1"
                     /translation="MVVRLNPVEFAKAMIKKKKQLIPTPIVLDNEIAGIVYGYYEGED
                     FYYLDRLDVDVSKKEELREMNVMELRQEIALKIKIFVANSN"
     gene            6188..6469
                     /locus_tag="ACZPDN_01715"
     CDS             6188..6469
                     /locus_tag="ACZPDN_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297373.1"
                     /translation="MCTVVHLEPEDFARELVHNQKNVYARTYVLDCGLAVIIYMCQDS
                     HFLYYLDRPDCSKEKKDMLKSMDFYELHAEIYRKVNLDNRLRERQNNPS"
     gene            complement(6481..7254)
                     /locus_tag="ACZPDN_01720"
     CDS             complement(6481..7254)
                     /locus_tag="ACZPDN_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR1297374.1"
                     /translation="MSIQKNKIIHINNEHIFGATTLKNIALPEKNNTALHVCIDPEAV
                     MINRKRLAEELNMPLDNWALPWQKHTNNMAHVTSSDKGKGAYDKNTSIMNVDAVYTTE
                     PNILIGVFTADCLGILVVDETTPCLCAIHSGWKGTTLEITKKCLSELIEKDLIHPKSC
                     HVYFSPSIQFDSLEVGMEVVEQMQQISIDTTPFIRYMPNKKAYIDNQGINIEMCKELG
                     VPAQNIHPSVYDTKKELDTCFSYRNDKQTGEHFTFGYIK"
     gene            complement(7235..7735)
                     /locus_tag="ACZPDN_01725"
     CDS             complement(7235..7735)
                     /locus_tag="ACZPDN_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HIR1297375.1"
                     /translation="MFEKVDIHSLNFNPFDKIGKDWLLISATKNGKTNTMTASWGTLG
                     HLWNKDVAIIFIRPQRYTKEFVDESETFTLSFFDDKHKELSYLGTKSGKDEDKISKVN
                     FHIEMVDGNPTFKEAKEVFICKKLYVGKLEKEHFLLPEIYEKNYPLDDLHYVYIGEIT
                     DVYSKK"
     gene            complement(7748..8434)
                     /locus_tag="ACZPDN_01730"
     CDS             complement(7748..8434)
                     /locus_tag="ACZPDN_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR1297376.1"
                     /translation="MWNRQQVKEQAKQIMKRNYWKMFVVTLLTGILTGGKTTIIERVQ
                     DFASNNLSYDAQPIFYSSNFQYIFYSFISVASILGILYTIFIGNVIVVGKNGYFIKNN
                     NENPELSEIFKGFKGNYLNVVKILFLMDLKTLLWLFLFIVPGVIKAYEYSMIPYLLAE
                     NPNLSADEAFSLSKQMTTGQKMDLFVLDLSFLGWIILGAICCGIGLLFVQPYPEATKA
                     EVYLILKHRI"
     gene            8821..9486
                     /locus_tag="ACZPDN_01735"
     CDS             8821..9486
                     /locus_tag="ACZPDN_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1297377.1"
                     /translation="MNSIDIESKKFLGQPKNFVSIFNALLFDGKQVLKPEYLKDENSE
                     LVMNVSSKHVDIIKRYEDGTYLDLFVIESQSYVDPSMVARVMEYESVARMRYIRQNFK
                     KHVSMHTRLPMILTVVLYVGESKWNAAKRLSELEIIHPGFEDMFNEFKLNLIELNTNH
                     KYNTGEKATQDFFDLLRMIYRKQILKEDLDREFNRDALYFVYVVTKNKELLSIYQKMK
                     RVM"
     gene            9489..9722
                     /locus_tag="ACZPDN_01740"
     CDS             9489..9722
                     /locus_tag="ACZPDN_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297378.1"
                     /translation="MCRALDEMFEESTNKGIQMGIKQGIEQGIEQGVKRGVKNTQIKI
                     AINMLVRNNQTLEEISEIVGLDLDALRELKKSI"
     gene            9722..9907
                     /locus_tag="ACZPDN_01745"
     CDS             9722..9907
                     /locus_tag="ACZPDN_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297379.1"
                     /translation="MEWMCGHIHFLQNVEKMFNKGIALNQFEVYQTMLDKGFSVKAIA
                     SIFSVSEELIKKIFIHV"
     gene            10037..11089
                     /gene="hrcA"
                     /locus_tag="ACZPDN_01750"
     CDS             10037..11089
                     /gene="hrcA"
                     /locus_tag="ACZPDN_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1297380.1"
                     /translation="MEITQRQKIIFKTIVEEFTRCAEPVGSKTLMELLDFPVSSATIR
                     NEMATLEKLGLLEKTHTSSGRIPSQKGYRYYVEHLMEADLDVSTKNALQQVFSERHYS
                     LEEVVQKSCDILSHMTNLTSVVLGPDSRHQTLQHVQLIPINNQSAVAIIVTDQGHTEN
                     KVFQFGTDVSMQDIQNCTDLLNDQLVGTPIENVVDKMKEIEPMMAAKLVRHEVLFEAF
                     VSAFMRFATEKVAVSGRSNMLYQPEFSDINKLKQMMRILENGNLFHQWTDQEGNVAIQ
                     IGERNELLQIGDCSVLTTKFHYNSDEEGQLMVVGPNRMQYSKVVALMDYMSNVIEDVF
                     GTDKNKGGANDEQRRK"
     gene            11070..11621
                     /gene="grpE"
                     /locus_tag="ACZPDN_01755"
     CDS             11070..11621
                     /gene="grpE"
                     /locus_tag="ACZPDN_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1297381.1"
                     /translation="MSKEENKEVKEEVVEEVAEETAEDVVEEKKEPDSDEIIEGLKAE
                     LDKAKNDVARAYADTENMKKRLQKDADTARKYRFQQPATEILPILDSMEMALKVQTED
                     EVIKNYVKGFEMIHKQLKGVLEKEGVQEIDVADKPFDHNTMQALMQEKKEGVESGMVI
                     EVLQKGYMLKDRILRPALVKVSE"
     gene            11635..13440
                     /gene="dnaK"
                     /locus_tag="ACZPDN_01760"
     CDS             11635..13440
                     /gene="dnaK"
                     /locus_tag="ACZPDN_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1297382.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGKDCKVIPNPEGNRTTPSVVAFKD
                     GERIIGDAAKRQAVTNKNTISSIKRLMGTNEKVEAEGKSYTPQEISAMILQYLKSYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIINEPTAAALAYGIDKTDK
                     EEKVLIYDLGGGTFDVSILDLADGTFEVLSTNGDTHLGGDDFDNVLVNWMVDTFKKDN
                     AIDLSQDPMAMQRLKEAAEKAKKDLSGVMQTQISLPFISAGPAGPLHFDTTLTRAKFD
                     DMTKHLVERTLIPIENALRDAKLSKSDIDEVLLVGGSTRIPAVQEAVRQALGKDPNHS
                     VNPDEAVAMGAAIQGGVISGDVNDVLLLDVTPLSLGIETMGGVMTVLIPRNTTIPTSK
                     SQIFSTAADNQPAVDIRVLQGERPMANDNKELGNFQLNGIAPARRGVPQIEVKFDIDA
                     NGIVHVSATDKGTGKEQHITIQNSSGLSDEEIDRMVREAEDNKAEDEKRKEEIDLRNK
                     AEGFIAQIDGMLEDNKDKIDPKQAEETKKLRDDLQKSLDENNMEEVKSKLDMLEKAAM
                     QAGQAMYNQPNGDAASQSTQTHSGDDVVDGEFTEK"
     gene            13508..14620
                     /gene="dnaJ"
                     /locus_tag="ACZPDN_01765"
     CDS             13508..14620
                     /gene="dnaJ"
                     /locus_tag="ACZPDN_01765"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR1297383.1"
                     /translation="MAEKRDYYEVLGVSKSATPDEIKKAYRKLAMKYHPDRNHEPGAE
                     DKFKEINEAYEVLSDEKKRATYDQFGHAGMDGAFGQGGGFSGGFTDFGDLGDIFGSFF
                     GGGFGGGGSRRSSNQPRQGQDRYMQMRIDFMDSIFGKTETISLDVDETCKHCNGSGAE
                     SPSDVQTCPTCHGSGYVMSQQRTPFGVIQQQSVCPDCHGTGKKVTHACSHCHGKGYEH
                     RRVKLDIKIPAGIQSGQQIRIPQKGERGSNGGPNGDLYIEIMVAPHPTFKREDNNIFI
                     KVPISSIDATIGCIIQVPTVYGDVELKIPEGTQPNTKFRLKGKGVKSRSGEGDEFVEV
                     QVEIPRKVSRRERELYEELKSGQAESPFERFKRAFK"
     gene            complement(14633..15064)
                     /locus_tag="ACZPDN_01770"
     CDS             complement(14633..15064)
                     /locus_tag="ACZPDN_01770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR1297384.1"
                     /translation="MEENMHALMERLNSSSQFLQSNDMRVTEVRHGFAKVEMIIDEQI
                     LNIHGFVHGGALFSLADTVAGAASFATGRDSVTLTGTINYIKPGRGGKLIGIAQEISS
                     GRTTGVYEVFIFNDENVLLSRATFTMFFLDSDKMKKKKEQK"
     gene            15144..15686
                     /locus_tag="ACZPDN_01775"
     CDS             15144..15686
                     /locus_tag="ACZPDN_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297385.1"
                     /translation="MDSNNIFFKVIVGVVLVGYMAFIFYQSLKGGIAYKKEFENFKNS
                     HKHYETMVDGGYWVWVMVAMAVVAFVLMFMAPKLASNGQTYYYYLAYGCIGLVFLGLS
                     IDSYTHRRVYFTEDGFFFEDTYYRYRMLANFEFKNGVITHNCTVLMSNRDKIVVTKKI
                     GEKLQEKQKAFKKAKKERHK"
     gene            15686..16459
                     /locus_tag="ACZPDN_01780"
     CDS             15686..16459
                     /locus_tag="ACZPDN_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR1297386.1"
                     /translation="MLFLSDLDGTLMNSNGTITEEDVNSIYQWTQKHSFGFVTGRDHA
                     FCMELASKYQLSCEYMITDNGANAYYKDQNVYSSLIDLDDGIQMCKEILKYNLDLFYT
                     DEVGNRYYPVSSYGKERLHSFEKKQPSLGRFSNIDILEFLNSRDLGLAKLSMYVENDL
                     DLLLNQFRDLFKNYEVMATSKDYIEITKKGTNKWEAFCHLPVEDTIFIGDGENDLCIL
                     KNLKNTYVMDHAPESLKVYGVCVKSVAQAIDIESRKENV"
     gene            16452..16778
                     /locus_tag="ACZPDN_01785"
     CDS             16452..16778
                     /locus_tag="ACZPDN_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297387.1"
                     /translation="MSRKRKKGAKATAASMVMDTLKKRGAVDEKSAVDVSAFKNLPYA
                     TSTISYTISNLIEEGVVGKTNDERFYYDDLGFKALETKFVRGYSMIFIVPIVIAILVY
                     VLQKVL"
     gene            16826..17806
                     /locus_tag="ACZPDN_01790"
     CDS             16826..17806
                     /locus_tag="ACZPDN_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1297388.1"
                     /translation="MEKPKSFAYIIFCLTICLAFAIGSNYLINTTTQSNMIYNSTEPR
                     KIGATYMTYNNTFYSVINEEISRILKEHGDQLIALDSAMSLKKQKEQIHYLIEQQVDA
                     IVIAPVDYEGLEDSLKEAYRNHIPVIVVDTEVKHNKNVTYSIVSDNYDAGVQCAKDMM
                     RKLDHANIVLLQHSTTRSGYLRIKGFEDTIQSNENYKVVKRMECDGQLEVAMPKMESF
                     IEEGIEFDVVMCLNDPSAMGAMAALSSNNMLKDKYVYGIDGTPEAKEMVAEGKMAATV
                     AQSPKTFGKKAGEVIYKLLSQETIKNKNEMSPVQIITKENVQDYSTEGWQ"
     gene            17803..>18190
                     /locus_tag="ACZPDN_01795"
     CDS             17803..>18190
                     /locus_tag="ACZPDN_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1297389.1"
                     /translation="MIQNIKKYNVWLVYVCMWVFSFFTVWYTAIVMYYTLVHVTKSGY
                     ASDFIKNISTLSNVPMRSFYISIFMFIALFCFVSIRPKLKFFYHHQMIPIFIELGFSL
                     ILMKNISFSATCILFIVIADCLMYVDK"
     assembly_gap    18191..18200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18201..19128
                     /locus_tag="ACZPDN_01800"
     CDS             <18201..19128
                     /locus_tag="ACZPDN_01800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1297390.1"
                     /translation="KPFDRSICIILVFLAYMFSNYGYLSNYIPMISFQEYLSVYNSRT
                     QGLLMGIEVTLTNLNIVLFIAYIFLYLQRQMNETQKFASLNAELKRLNIQLKGYANLR
                     EKMGETKERNRLAREIHDTLGHTLTGLSVGLEACQVMIDRDVNVTKAQLGILQESAKR
                     GLTDVRRSVDKLKPDALERYSLKEALDMMIMDFQKLTDVTILYLCHLPLVNLNADEEE
                     IVYRVVQEGMTNSVRHGHATKIYVSIAQADNNLIIIIEDNGQGCKDVNPGFGIHHLTE
                     RINLLQGRIRYYGDNGFELIVEIPMRRGENNG"
     gene            19121..19774
                     /locus_tag="ACZPDN_01805"
     CDS             19121..19774
                     /locus_tag="ACZPDN_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1297391.1"
                     /translation="MVKIMIADDQQLIRESLKIILDSDPSFEVVKVVGSGQEVLDSLK
                     TESVDIILMDVRMPNMSGVDCTRIVKKEYPNIKVIILTTFDDDEYIYDALKYGASGYL
                     LKGCSLEELSSAIHSVLSGGACFNPNVAGKVAQFFSQMANSNSEFSLEHEEDFDLSDT
                     ERQIIAQIGHGYSNKEIAQKLGFSEGTIRNYLSVILDKLDLRDRTQLAIWYLSRGNK"
     gene            19774..21090
                     /locus_tag="ACZPDN_01810"
     CDS             19774..21090
                     /locus_tag="ACZPDN_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1297392.1"
                     /translation="MVRKRLRTFLIAILVCCLAATFYYTQKPIVLTIGVFAGSNWNVP
                     SPYSGKIIDNVIERFEKTHPNVQVEYVSGILKEDYSDWVSKEALNGKLPDVFMVLSDD
                     LSTYAKVGMLEPLDTFMQTDADFNKNRYFSTTLNAGNIYDHQYALPYESSPTLMFVNK
                     TLLEENGIAIPNVDWTWDDFYSICEKLTVDTDGDGEIDQFGEYGYTWRNALSSNGQAL
                     YDEESMKCTLKKQDVYSAIEFVRQLNRLNRNQNVTSELFDKGKIAFCPMMFSEYRTYK
                     PYPWSVKKYSSFEWDCLPMPAGPSGNNVSQMDSLLCGISKMSSKKKMAWEFLKLLCYD
                     ETTQESLFKYSQGVSVLKNVSTSKNVTKYIQEDAPLDSNYNLDFFDDMMEQAITIKKL
                     DGNDQLYSMADSEIRRVIDTNEDIELAMQNLNDELNILLEKQSLTL"
BASE COUNT         7181 a         2984 c         4137 g         6864 t
ORIGIN      
        1 gcatttgaat tctacaatga aagaaatgtt gaagattgta ttcaaattcc atgggataca
       61 attgatcgag tagaagcgag tgtcttattt aacaaaagta tttctcgatt tgtgattttc
      121 ataaatgaga aaacatactt tactttctct acaaaagaca acaaaaaaac acttcgtgct
      181 ataagagaat atgtacctgc agatcgaatg cttcgatcat tgagtttttg ggatgtgttt
      241 aaaagaggtt taatgtatat tccaaataaa ttactacatt ctaataacta agtattgtta
      301 acccaatccg caatatgctg attgggtttt ctttctttat gataatccga aaaagtacaa
      361 gatgtataac agccattaaa gaattagact ttggtttaat tgtacaggtt gcatatataa
      421 agattatatc taatgaatta agatcgatgt tcgtaaggtt ccaacagatt cacctgaaaa
      481 cttcgatgaa attcttaaaa aggcaaaatc agaattaaag atggcttagg tcatcttttt
      541 aaattattga aaagtcataa aaagaacact aaaatatatt gaaaagtcat taaaatgtgt
      601 ataaaattat attgaaaagt cattttagag gtgaaatatg ctttatcgaa aggttgaacg
      661 tacaattgaa caacatttaa agaatggttc aaacaaaatt ttgttagtgg atggagctcg
      721 tcaaattggg aaaacatata tcataaggta tgtgggaaat aaactgtttg aaaattatat
      781 cgaaatcaat atgattgaag actctttagg aaatcgttcg tttgaaaagg taaggacagt
      841 agaagatttt tatctgcagg tcagtatgct tgcgggggct aaaatgcaag aaaaagaaaa
      901 cacattgata tttatcgacg aaatacaggc atatcctcat ttattaacat tattaaaatt
      961 tttatcccag gataatcgat ttacatatat tgcgagtggt tctttattag gagttacttt
     1021 atcgcaaacc acatcgattc ctatgggtag tattcaaaag attcaaatgt atcctctgga
     1081 ttttgaagaa ttcttgtatg cgaatggcat gaatgaattt gtagtttcga cattaagaaa
     1141 taaatttaat catttagaag aattagatga gtccatgcat aataaaatga tggatttgtt
     1201 taaaaaatat ttgttggtgg gtggtatgcc tgacgctata aacacgtatt tgtcagagaa
     1261 gaatattgta tctgttcgaa atattcaagc tgaaattcac gaatattatg catcagatgc
     1321 ctctaaatat agtttagaaa ataaacttaa aatcagtcga atttatgatt taattccatc
     1381 taatatggaa aataaaaaaa agcgagttgt tgttaaaaat attgagggaa aaagaggtaa
     1441 aacgtttaat gactatcaag aagaatttga atatttagtt agtgcaggaa tcgctttgaa
     1501 tgtacaagct atctcaaatc caaaatttcc tttgattgaa tctgcaagta agaatttaat
     1561 gaagttatac ttaaatgatg ttgggatttt aacgggcata ttatatggaa ataatattaa
     1621 tgcgatttta gatgatcaaa aaagtattaa tttaggctct gtatatgaaa caattgttgc
     1681 cagtgaatta aaggcacatg gcaaaaaatt gtattactat gataatcgaa ataaaggaga
     1741 agttgatttc ttgattgatg attatgattc actttctgtt gttcccattg aggtgaaatc
     1801 aggaaaagat tatacggtgc atagtgcatt gaataatttt gtgaataata atgattacca
     1861 tattcaaaaa ggctatgtcg tatcgaatga gagaacaata aggcaaaaag gtaaaatcat
     1921 ttacttgcca atctattaca taatgttttt ttaaacaagg atgtggattt ctagaattcc
     1981 acatccttgt tgtttaaaat atgatcaaat atataatgtc caacaccacc ttcaagacaa
     2041 tcataatcgg taatgtcgtc ggccagtgat tttgtgtttg gattgccatc ctttagacag
     2101 acgccccatc cacttgtgag tagtaaagaa ttatcgtttt cattatcacc aaacgcaatt
     2161 gtattttcta agtggatttg attccgttcg caatacattt ctaaacctct acctttagag
     2221 aaaccactat acattaattc gatagttcct gggaatgttt caatagtttg gtatttccct
     2281 ttatattgtt cttctacttg atcacggata agttgcgttg tttctgggtc tgttctaaat
     2341 aagaacttat aaacaggttt ttcacaaaaa ccatcaatat cacctgtttt atcaataagt
     2401 ggaatgtttc ttcgttttga catatctaac aaatcctcgc tgatattcat aacgttgcgt
     2461 agttgatttc cttcaacgtt tatagacact ttattttctt caatgattgg catcaagaaa
     2521 gttaagactt ctttcatttc ttcaatactc atcatgtcca ttgtaaatgt cttatccagg
     2581 tttcgatcat agatcatgcc accattcatt ccgattatga aatcaaattc aaaccctaat
     2641 ttccagaata ttccttttgt tttttccatt tcggtgattt ctcttccggt tgccaaacca
     2701 agcttaacgc ctctattatg caattcttgt aaagcctgta ttgtgatttc aggtaaagga
     2761 ctgcttttcg aacttaaagt catatcgacg tcagcagcca agaccttaat tttattaatc
     2821 ataaaacgtg cctccatcac ataaaatgta tacgatttca tatgaagttg caacttgaaa
     2881 gttatattgt actacctgtt tcgttttgta ttttgtcttt aggtaagcta tactttagag
     2941 acgtgaaggt ggtaaataat gaatttttta gtgcgtagat ttataaagaa ttatcaagat
     3001 acaaaagatg ccaatgtaag aacaaatatt ggtaaattat cagggattgt gggtatattt
     3061 agtaacttat ttttatttgt gattaagttt gtgattggta ccattgtaca ttcggtatct
     3121 attcaagctg acggagtaaa taacttaaca gatgcgggtt cgaatattat ttcgatttta
     3181 tcttttcatc tcgcaaataa gccagcagat aaggatcatc catttggtca tgaaagaacg
     3241 gaaacaattg cgtctttatt tgtaggtata ttgattttag ttcttggttt tgaaaccgca
     3301 aaagatagta tttctaaggt gattcatccg ggaagtattg attttagaat tgcatccgtc
     3361 attattcttt taatttctat tatagttaaa ttttggatgt atgcatacaa taagaaactt
     3421 tctaaaacat atgattcaag tctattagaa gcaacggcat tagattcaat tagtgatgta
     3481 tgtggaacaa cggcagtact tgtatctaca ttattatctc ctgttttaca ttttaatcta
     3541 gatggatata tgggtattgt agtttcagga attatccttt atggagctta tggcttatta
     3601 agagatatga ttaattcttt aattggagaa gcaccagatc ctgaacttgt acacaatatt
     3661 gtagatagga ttatggcgca tcctgcaatt ttaggtgtac atgatatgat gctacataat
     3721 tatggtccaa ataagatttt tgcgagtgcc catgtagaag tggattcttc aaaagatatt
     3781 tttgaaacgc atgatcatat tgataatata gagcgtgaag ttaaagaaaa tatgaatatt
     3841 gatttagttt tacatatgga tccagtaaag gtaaatgatc cagaaacaga gttgtaccgt
     3901 gcaaaagttg tagaagctat tcatcaaatt gatccaaaat ggggatttca tgattttaga
     3961 attgtgtcag gaccaacaca tgtgaatttg gtgtttgatt tggtgattcc gtttgaagaa
     4021 aagtattcac aagaagaaat agaagatatg cttctaaagc atattcaatc ggataaaaag
     4081 atttatttag tactcacaat tgatcatcca tacgcataag gcagaaggtt ttaaacttct
     4141 gcctctatta tttctgggat aacttggatt tgagatttat tctctttagt taatgtattt
     4201 gaaataaata catccacttc tttgaattta cagtatgtgt attctggttg tgcattaaat
     4261 ttatttttgt cagttaccac aatgattttt tcactttgtt gaagaatatg tcgctttaat
     4321 tcatattcat tttcataagt ggtaaatccg tcgttgtctt taaatccttt agttcctaaa
     4381 atcgcaacat taatatgaat ataatctata gtttcactgg cgaagtttcc ataagagcga
     4441 taggcattgt tggaaatgtt tccaccaatt aaaataacgt gcatgtttag tttagcgcat
     4501 ttgtaggcta caaagatgga gtttgtcaca atcgtaatat tatgaatgga ttgaagaaaa
     4561 ggaatgcctg caagacatgt actaccggca tctaaataga caaccattcc ttctttgata
     4621 aaggtgcagg ctttcttcat gatttgtttc ttttcctcaa tatattcttg atttcttaat
     4681 gaaatggaca tttcatcagg ttcttctaaa agcagcgcat agccacgctc tttttttatg
     4741 attttagaat cttcaagttc aataatatct ttacgaatgg tttcacttga agtttgaaag
     4801 taatcagcta aatcggatat cttgattttt ggatgttcct gcagaagttg acgaatcttg
     4861 aattgtcttt catctttctt ttttgacata agctacacct gtgtgacttt cttgactgtt
     4921 tttacaacat catattgttc tgctgttaaa tggtttgtga tgaattcatc aaactcatta
     4981 aatttacaaa aggcataagg cgcaatatct gccttattat taactttact tgcatcacta
     5041 atcatgattt tttttgcgga ttgattcata acatgacgtt taaaaccaag ttcatcggct
     5101 gaaatggtgg taaatccgtg actcttttca gtgaaagcat ctgttcccat gatggctaaa
     5161 tcaatgctta aatggtcgat gatttcggtt gcgaaatgtc cataggttct tttccctata
     5221 ttaaataata aaccaccagc catgatgatg tttaaattca tgttggcaca ttcataagct
     5281 acaagaatag agttggtgac aatggtgatg tcacttttgt ttcgtaaagc gcttatagcc
     5341 gttaaaattg tgcttccaga atctaaatat acgatcatgc cattttcaat tcggctaaat
     5401 gcttctaggg ccacaatttt tttcatttct ttattttttt gtgcttttaa ttcgacaaat
     5461 gtttcgacag aagaactttg aatcttggcg tatccatgtt ctcgaattaa tatttttctt
     5521 tcttgtagtt catctaaatc tttacgtagt gtttctggag taacagaaag taatttggca
     5581 agttctgcga cttttatttt ctcttttcct tcaagaattt gacgaatttg aagttgtctt
     5641 tcttctttct tctttgacat gttttcacct tttttctttt gaatccaatt aaattataaa
     5701 cgaaatattt tgaaaaaaga ataaaaagtg ggaatacaaa taatttgaaa gtgagtaatg
     5761 aattcaatat tgtttctgtg cataatgaga ttgtctaaag aaagtatgtg atagatatgg
     5821 tagttcgatt gaatccggtt gaatttgcga aggcaatgat aaaaaaaaag aaacaattga
     5881 ttcccacacc gattgtttta gataatgaaa ttgccggtat tgtgtatgga tactatgaag
     5941 gtgaagattt ttattatttg gatcgtcttg atgtcgatgt ttctaaaaaa gaagaattaa
     6001 gagaaatgaa tgtaatggaa ttgcggcaag aaatagcatt aaagattaaa atttttgtag
     6061 ccaactcaaa ttgagataaa atcttttgaa tcttaaataa aaacttggaa agctaaataa
     6121 ttaaattaaa ctattgagaa gaatccacaa ttcatacata atgcaggtgt caaagggggt
     6181 ataagaaatg tgtactgtcg tccatcttga accagaagat ttcgcaagag aattagttca
     6241 taatcaaaaa aatgtttatg ccagaacgta tgtcctcgat tgtgggcttg ccgtaattat
     6301 ctatatgtgt caagattcgc atttcctata ctacttagac agaccggatt gttcgaaaga
     6361 aaaaaaggac atgctgaaat cgatggattt ttatgaactg catgccgaaa tttatcgtaa
     6421 ggtcaattta gataatcgtc ttcgtgaaag gcaaaataac ccaagctaga ttgcttgggc
     6481 ttatttaata tatccaaagg taaagtgttc gcctgtttgt ttatcattac gatagctaaa
     6541 acatgtatcg agctcttttt ttgtatcata tacacttgga tgaatgtttt gtgccggaac
     6601 ccctaattcc ttacacattt caatattgat accttgatta tcaatatagg cttttttatt
     6661 tggcatataa cgaatgaatg gtgtcgtatc aatactgatt tgttgcattt gttcaacaac
     6721 ttccatacca acttctaaag aatcaaattg aatacttgga gaaaaatata cgtgacaaga
     6781 tttggggtga atcaaatctt tttcgattaa ttcactcaag catttttttg tgatttctaa
     6841 agtagttcct ttccatccag aatgaatagc acataggcat ggcgttgttt catcaacgac
     6901 aagaatgcct aagcagtcag ccgtaaatac accaattaga atgtttggct ctgtcgtata
     6961 tacggcatct acattcataa tgcttgtatt tttgtcatag gcacccttac ctttatcact
     7021 agaagtaacg tgggccatat tgtttgtgtg cttttgccaa ggaagtgccc agttatctag
     7081 aggcatattt agttcttcag ctaatctttt gcggttgatc ataacagctt ctggatcgat
     7141 acatacatgg agtgcggtat tgttcttttc aggaagagca atatttttta gtgtagtagc
     7201 accaaatatg tgttcgttat tgatatgaat aattttattt ttttgaatag acatctgtaa
     7261 tttctccaat atatacatag tgcaaatcat cgagtggata gtttttttcg taaatttcag
     7321 gcaataagaa atgttctttt tcgagttttc caacatagag cttcttgcaa ataaagactt
     7381 ctttagcttc tttaaatgta ggatttccat ctaccatttc aatatggaag ttcacttttg
     7441 aaatcttatc ttcatcctta cctgattttg tgcctaagta agataattct ttgtgtttat
     7501 catcaaagaa ggatagggta aacgtctcac tttcatcaac gaattctttt gtgtatcttt
     7561 gtggtcgaat aaagataatc gcaacatcct tattccataa gtgaccaagt gtcccccagg
     7621 aagcggtcat agtgttggtt ttaccgtttt ttgtagcact gataagtagc cagtcttttc
     7681 caatcttatc aaaaggattg aagtttagac tatgaatatc aactttttca aacatatata
     7741 attcctccta tattctgtgt tttaaaatca aataaacttc ggcttttgtt gcttctggat
     7801 atggttgtac aaatagaagt ccaattccgc aacaaattgc gcctagaatt atccatccta
     7861 aaaaagataa atctaagaca aatagatcca tcttttgtcc agtcgtcatt tgtttggaca
     7921 aagagaaggc ttcatcagcc gaaagatttg gattttctgc caatagatat ggaatcatag
     7981 aatattcgta agctttaata actccaggta ctatgaataa gaatagccat agtagggttt
     8041 ttaaatccat taaaaatagg atttttacca catttaaata attgccttta aaccctttga
     8101 atatttcact tagttcagga ttctcgttat tattctttat aaagtatcca ttttttccaa
     8161 ctacaataac attaccaata aagattgtat agaggattcc taaaatacta gcaacagaaa
     8221 taaaggaata aaatatatat tggaaattag atgaatagaa tataggttgg gcatcataag
     8281 aaagattgtt tgaagcaaaa tcttgtactc tttcaataat cgttgttttt ccgccagtta
     8341 atattcctgt aagcagtgtt accacaaaca ttttccaata gttgcgtttc atgatttgtt
     8401 tagcttgttc tttaacttgt tgtcgattcc acatagggag caccttcttc cttatatttt
     8461 aattttatca tataagaaaa tgaatgatgt ggaaaaagat atatgattgt atggttcgta
     8521 taaagatttg catgcatata cttaaaaaca aagaaaccat tttaagtgtt ggagtcttaa
     8581 aatgggtcct ttttgtttat tattttttta ggagagagat atgaatattg gtttatttct
     8641 atattcattg ccttatcttt aaggcactta tagaatatca ttctatgttg ttatgaattt
     8701 agtataactt tggtaaatgt tgtgaaatca gattgaatta tgtgaaattg tcaaaatgtg
     8761 cagcttttct aaaatttcct tctttttttt cgtattagag agaagaaagg aggagctatc
     8821 ttgaattcga ttgatattga gtctaagaaa tttttaggac aaccaaaaaa ctttgtctcg
     8881 atctttaatg cacttttatt tgatgggaag caagtgttaa agcctgaata tcttaaggat
     8941 gaaaacagtg aacttgtcat gaatgtttcg agtaagcatg tggatatcat caagcggtat
     9001 gaggatggta catatttaga tttgttcgtg attgaaagcc aaagctatgt agatccgtcc
     9061 atggtcgcaa gagttatgga atatgaaagt gttgctcgta tgcgttatat tcgtcaaaac
     9121 tttaagaagc atgtatctat gcatacaaga cttcccatga ttttaacagt tgtcttgtat
     9181 gtgggtgaat cgaagtggaa tgcggctaaa agattgagtg aactagaaat cattcatcct
     9241 ggatttgaag atatgttcaa tgagtttaaa ctgaatctga ttgagctaaa cacaaatcac
     9301 aagtataata caggtgaaaa agcaacacag gacttttttg atctattgcg tatgatttat
     9361 agaaaacaga ttttaaaaga agatctagat cgagagttta atcgtgatgc attgtatttt
     9421 gtgtatgtag tgacaaagaa caaagagtta ttaagtatct accagaaaat gaaaagggtg
     9481 atgtaaaagt gtgtagagca ttagatgaaa tgtttgaaga atcaacaaac aaaggaatcc
     9541 agatgggtat caagcaaggc attgaacaag gcattgaaca aggtgttaaa cgtggtgtaa
     9601 aaaatacaca aatcaaaata gcaattaata tgcttgtaag aaataatcaa acgcttgaag
     9661 aaatctctga gattgttgga ttagacttag atgcattaag agaattaaaa aagagtattt
     9721 gatggaatgg atgtgcggtc acatccattt tttacaaaat gttgagaaaa tgttcaacaa
     9781 aggcattgca ctaaatcaat ttgaagtata tcaaaccatg ttagataaag gtttcagtgt
     9841 taaagctatc gcgtcaattt tctcagtgag tgaagaattg attaaaaaga tttttattca
     9901 tgtttagtga taggggtacg aatattcgta tccttaattt ttggttgaaa tttagcactc
     9961 aattgttgac ggtgctaaaa attgtgatat actaatagca gacaaggaaa atgagtgcta
    10021 gaaggaggtc ttcacaatgg aaatcacaca acgtcaaaag ataatcttta aaacaatagt
    10081 tgaagagttc actcgttgtg cagaaccagt tggttcgaag actttgatgg aacttctaga
    10141 tttcccggtt tcatctgcaa ccatccgtaa tgagatggcg acacttgaga agttagggtt
    10201 gttagagaag acacatactt caagtggtcg aattccgagt caaaaaggct acaggtacta
    10261 tgttgaacat cttatggaag cagatttgga tgtttctaca aagaatgctt tacagcaagt
    10321 ctttagtgaa agacattatt cgttagaaga agttgttcaa aaaagctgtg acattttgtc
    10381 acatatgaca aatttaacga gtgttgttct aggacctgat agcaggcatc agactttaca
    10441 acatgtacag ttgattccaa tcaacaatca aagtgctgtt gcgatcattg tcacagatca
    10501 aggacataca gaaaataaag tgttccaatt tggtacagat gtatctatgc aggatattca
    10561 aaattgtaca gatttattaa atgaccaatt agtgggaacg ccaattgaaa atgttgttga
    10621 taaaatgaaa gaaattgaac cgatgatggc ggcaaaactt gttcgacatg aagttttgtt
    10681 tgaagcgttc gtttcagcct ttatgcgatt tgcgactgaa aaagttgcgg tttctggacg
    10741 aagcaatatg ttgtatcagc cagaatttag tgacattaac aagttgaaac aaatgatgcg
    10801 tatattggaa aatggaaatt tattccacca atggacggat caagagggaa atgtagcaat
    10861 ccagattggt gaaagaaacg agttgttaca aattggagat tgttcggtat taacaacaaa
    10921 attccattat aacagcgatg aggaaggaca attaatggtt gttggtccaa atcgaatgca
    10981 gtattccaag gtcgtagcct tgatggatta tatgtcaaat gtcatcgaag acgtctttgg
    11041 gacagacaaa aataaaggag gtgccaacga tgagcaaaga agaaaataaa gaagtaaaag
    11101 aagaagttgt tgaagaagta gctgaagaaa cagctgaaga tgtagttgaa gaaaagaaag
    11161 aacctgattc agacgaaatc attgaaggtt tgaaggcaga acttgacaag gcaaaaaatg
    11221 atgtagcacg tgcttatgca gatacagaaa acatgaagaa acgtctacag aaagatgcag
    11281 atacagctcg aaaatatcgc ttccaacagc cagcaacgga aatcttgcca attctagatt
    11341 caatggaaat ggctttgaaa gtacaaaccg aggatgaagt gattaagaat tatgtcaaag
    11401 gctttgagat gattcataaa caattgaaag gtgtgctgga aaaagaaggc gtacaggaaa
    11461 tagatgtagc cgataaacca tttgatcaca atacaatgca ggctttaatg caagagaaaa
    11521 aagaaggcgt tgaaagtgga atggttattg aagtattaca aaaaggatat atgttgaagg
    11581 atcgtatttt acgacctgca ttagtaaaag taagcgaata gggaggtaat tatcatgagt
    11641 aaaattattg gtattgactt aggtacaaca aatagctgtg ttgcggttat ggaaggtaaa
    11701 gattgtaaag taattcctaa cccagaagga aatcgtacaa caccaagtgt tgttgcgttc
    11761 aaagatggag aacgtattat tggtgatgct gcaaaacgtc aggcagtcac aaataaaaac
    11821 acaattagtt caattaagcg tttgatggga acaaatgaaa aagtagaagc agaaggaaaa
    11881 tcatatacac cacaagaaat tagtgcaatg atccttcaat acttgaaatc atatgcagaa
    11941 gactacttag gagaaaaagt aacaaaggct gttatcacag ttcctgcata cttcaacgat
    12001 gcacaacgtc aagcaactaa agatgctggt aaaattgctg gattagaagt agaacgtatt
    12061 atcaacgaac caacagctgc agcattggct tatggtattg ataaaacaga taaagaagaa
    12121 aaagtattga tctatgactt aggtggaggt acttttgatg ttagtatcct tgatttagca
    12181 gatggaacat tcgaagtatt gtcaacaaat ggagacacac acttaggtgg agatgacttt
    12241 gataacgtat tagtaaactg gatggttgat acattcaaga aagataacgc aattgacttg
    12301 tcacaagatc caatggcaat gcaacgtcta aaagaagctg ctgaaaaagc gaaaaaagat
    12361 ttaagtggtg taatgcagac acaaattagc ttaccattta tctcagccgg tcctgctggt
    12421 ccattgcact ttgatacaac attaactcgt gcaaaatttg atgatatgac taaacattta
    12481 gtagaacgta cattgattcc aattgaaaac gcattaagag atgctaaatt gtctaaatct
    12541 gatattgacg aagtattatt agttggtgga tctactcgta ttccagctgt tcaagaagca
    12601 gtacgtcaag cattaggaaa agatcctaac cactcagtaa acccagatga agctgtagct
    12661 atgggtgctg caatccaagg tggagttatc tctggtgatg taaatgacgt attattacta
    12721 gatgttacac cattatcatt aggtattgaa actatgggtg gagttatgac agtattaatt
    12781 cctcgtaaca ctacaattcc aacaagtaaa tcacagattt tctcaacagc tgctgataac
    12841 caacctgctg tagatatccg tgtattacaa ggtgaacgtc caatggctaa cgacaacaaa
    12901 gaattaggta acttccaatt aaatggaatc gcacctgctc gtcgtggcgt tcctcaaatc
    12961 gaagttaagt ttgatattga tgccaacggt attgtacatg taagtgctac agataagggt
    13021 actggtaaag aacaacacat cacaattcaa aactctagtg gattgagtga tgaagaaatc
    13081 gatcgtatgg ttcgtgaagc tgaagataac aaggctgaag atgaaaaacg taaagaggaa
    13141 attgatttac gtaacaaagc tgaaggtttc attgcacaaa tcgatggaat gttagaagac
    13201 aataaagata agattgatcc aaaacaagct gaagaaacta agaaattgcg tgatgatcta
    13261 caaaaatctt tagatgaaaa taacatggaa gaagtaaaat ctaagttaga catgcttgaa
    13321 aaagctgcaa tgcaagcagg acaagcaatg tacaaccaac caaatggtga tgcagcttca
    13381 caatcaacac aaactcattc aggtgatgat gtagttgacg gtgaattcac tgagaagtaa
    13441 aataaataag caacatttga agttctagcc acgctagaac ttcttcgtat cgtatggagg
    13501 gattcacatg gcagaaaaac gtgactatta tgaagtgctt ggtgttagta aaagtgcgac
    13561 accagatgaa attaaaaagg cctatcgaaa attggccatg aaatatcacc ccgatcgaaa
    13621 tcatgaacct ggagctgaag ataagttcaa ggaaatcaat gaagcttatg aagtattaag
    13681 tgatgagaaa aagcgtgcta cctatgatca atttggtcat gcgggcatgg atggagcctt
    13741 tggacaaggt ggtggattct ctggtggatt cacagacttt ggagatttag gagacatctt
    13801 cggttcgttc tttggtggag gatttggcgg tggaggatct cgccgttcaa gcaatcaacc
    13861 tcgacaagga caagaccgtt acatgcagat gcgcattgat ttcatggatt caatttttgg
    13921 caaaacagaa acgatttcat tggatgtcga tgaaacatgt aaacactgta atggatctgg
    13981 agctgaaagc ccaagtgatg tgcagacatg tcctacatgc catggttctg gatatgttat
    14041 gtctcaacaa agaacacctt ttggtgtgat tcagcaacaa agtgtatgtc cggattgtca
    14101 tggtacaggt aaaaaggtta cacatgcatg ctcacattgt catggcaaag gatatgagca
    14161 tcgtcgtgta aaattggata ttaaaattcc agcaggaatt caatctggtc aacagattcg
    14221 tattccacaa aaaggcgaaa gaggatcaaa cggaggacct aatggagatt tatatatcga
    14281 aattatggtt gcaccacatc caactttcaa acgcgaagac aataatatct tcattaaagt
    14341 tccaattagt tcaattgatg caacgattgg ttgtataatt caagttccaa ccgtgtatgg
    14401 tgatgttgaa ttgaaaattc ctgaaggaac acaaccaaac actaaattcc gtctaaaagg
    14461 aaaaggtgta aaatcgcgtt caggagaagg ggatgaattt gtagaagttc aggttgaaat
    14521 tccacgtaaa gtatctcgaa gagaaagaga gttatacgaa gaattaaaat ctgggcaagc
    14581 tgaatctcct tttgaaagat tcaaaagagc gtttaagtaa atgaaaagag cattatttct
    14641 gctctttctt ttttttcatc ttgtctgaat ctaagaaaaa cattgtgaat gtagctcttg
    14701 aaagaagtac attttcatca ttaaagataa atacttcata aactccggtt gttcttccgc
    14761 ttgaaatttc ttgtgcaatc ccaatcaatt tgccaccacg accaggctta atataattga
    14821 ttgtacctgt aagtgtgacg ctatctcttc ctgtcgcaaa gcttgctgct ccagcgaccg
    14881 tatctgccaa agagaatagg gctccaccat ggacaaagcc atgtatattt agaatttgtt
    14941 catcaataat catttctact ttcgcaaaac catgtcgtac ttctgtaaca cgcatatcat
    15001 tactttgaag aaattgactc gaactattta aacgttccat taacgcgtgc atattttctt
    15061 ccataaacta cctccaacaa tgcttttatt atagcatgaa atgatgatat aatgcttatt
    15121 gtggttaaga aggagaatga tttatggatt caaataatat attttttaaa gttattgtgg
    15181 gtgtagtatt agttggatat atggcattca ttttttatca aagcttaaaa ggtggaattg
    15241 cctacaaaaa agaatttgaa aactttaaaa attcacataa acattacgaa acaatggtag
    15301 atggtggata ttgggtatgg gtcatggttg cgatggctgt tgtggcattt gttttaatgt
    15361 ttatggcacc aaaattagct tccaatggac aaacctatta ttactattta gcgtatggat
    15421 gtattggatt ggtattctta ggattgtcta ttgattcata tacacatcgt cgtgtttatt
    15481 ttacagaaga cggcttcttt tttgaagata catattatcg ttatcgcatg cttgcaaatt
    15541 ttgaatttaa aaatggtgtc attacacata attgcacagt gttaatgagc aatcgcgata
    15601 aaattgttgt tactaagaaa attggtgaaa agctacagga aaaacaaaaa gcatttaaaa
    15661 aagcaaagaa agagcgtcat aaataatgtt gtttttatcc gatttagatg gaactttaat
    15721 gaattcaaat ggaactatta cagaagaaga cgtgaattca atatatcaat ggacacaaaa
    15781 acattcattt ggatttgtga caggaagaga tcatgctttt tgtatggaac ttgcttctaa
    15841 atatcaattg tcatgtgaat atatgattac agataatggg gcaaacgcct attataaaga
    15901 tcagaatgtg tattcttctt taattgattt agatgatgga atacaaatgt gtaaagagat
    15961 cctaaaatac aatttggatt tgttttatac agacgaagta ggaaatcgct attatcctgt
    16021 ttcatcttat ggaaaagaaa gacttcatag ttttgaaaag aaacaaccga gtttaggacg
    16081 attttcaaat atagatatat tggaattttt aaattcacga gatttagggt tagccaagtt
    16141 aagtatgtat gttgaaaatg acttagattt gttattgaat caatttagag acttgtttaa
    16201 aaactatgaa gtaatggcta cttcaaaaga ttatattgag atcacaaaaa aaggtacaaa
    16261 caaatgggaa gccttttgtc atttacctgt cgaagatacg atttttattg gagatggaga
    16321 aaatgactta tgcatattaa aaaacttaaa gaatacatat gtcatggatc atgcacctga
    16381 gagtttgaag gtatatggcg tgtgtgtaaa gagtgtagct caagctatag atatagaaag
    16441 taggaaagaa aatgtctaga aaaagaaaaa aaggagcaaa ggcaacagct gcttcaatgg
    16501 taatggatac tttgaaaaaa agaggggcag tagatgaaaa aagtgcggta gatgtgagtg
    16561 cgtttaaaaa tttgccttat gctacttcaa caattagtta tacaatttcg aatctaatag
    16621 aagaaggtgt tgttggtaaa acaaatgatg aaagattcta ttatgatgat ttaggattta
    16681 aagctttaga aacaaaattt gtacgtggat attcaatgat ttttattgta cctattgtta
    16741 tcgcaatcct tgtatatgtt ctacaaaaag tgttataaaa aagtgtcgca gtaatgcgac
    16801 attttctgta aggaaggaga tttgaatgga gaagccaaaa tcatttgcgt atattatctt
    16861 ctgtttaacg atttgtctag cctttgcgat tggcagtaat tacttgatta atacgactac
    16921 gcaatcaaat atgatatata attcgacgga gccacgtaag ataggagcta cttatatgac
    16981 ctacaacaat acgttttatt ccgtaatcaa tgaggaaatt tcaagaatct taaaagaaca
    17041 tggggatcag ctcatcgctt tggattcagc tatgagttta aagaaacaaa aggaacaaat
    17101 tcactatttg attgaacaac aagtcgatgc catagtaatt gcgcctgttg attatgaggg
    17161 gttagaagat tcattaaaag aagcatacag aaatcatatt cctgtgattg tagtggatac
    17221 ggaagtaaaa cataataaga acgttactta tagtattgtc agtgataact atgatgcggg
    17281 tgttcaatgt gcaaaggata tgatgcgcaa gcttgatcat gctaatattg ttcttttaca
    17341 acattccacg actcgttcag gctacttacg tattaaagga tttgaggata ccattcaatc
    17401 caatgagaac tataaagtgg tcaagcgtat ggagtgtgat ggacaattag aggtggccat
    17461 gcccaaaatg gaatctttta ttgaggaagg aatcgaattt gatgttgtaa tgtgtttgaa
    17521 tgatccaagc gcaatggggg caatggctgc actttcatca aataatatgt tgaaagataa
    17581 atatgtttat ggcattgatg gaacgccgga ggcaaaagaa atggttgcag aaggcaaaat
    17641 ggctgcgact gtggcacaat cgccaaagac gtttggaaaa aaggctgggg aggtaattta
    17701 taagttgctt tcgcaagaaa caattaagaa taaaaatgaa atgagtcctg tgcaaatcat
    17761 cacaaaagag aatgttcaag attattctac ggagggctgg caatgataca aaatattaaa
    17821 aaatataatg tatggcttgt ctatgtctgt atgtgggttt ttagtttttt tactgtgtgg
    17881 tacaccgcaa ttgtgatgta ttatacgttg gtacatgtga caaaaagtgg ctatgcatcc
    17941 gattttataa aaaatatatc tacgctttcc aatgttccaa tgcgttcttt ttatatctct
    18001 atatttatgt ttattgcgtt attttgtttt gtgtccattc gtccaaaact caaatttttt
    18061 tatcatcacc aaatgattcc tatctttatt gagttaggat tcagtttgat tttgatgaag
    18121 aatatctcct ttagtgcaac atgtatctta tttatcgtca ttgccgattg tttgatgtat
    18181 gtagataaac nnnnnnnnnn taaacctttt gatcgttcaa tttgcatcat acttgtcttt
    18241 ttggcatata tgttttcaaa ttatggatat ttatccaatt atattcctat gatttcattc
    18301 caggagtatt tgtctgtata taattcaaga acacaaggct tattgatggg tattgaggtt
    18361 actttaacca atttaaatat tgtattattt attgcctata tattcttgta tctacaaagg
    18421 cagatgaatg aaacacaaaa gttcgcttca ttaaatgctg aattgaaacg cttaaatatt
    18481 caactaaagg gttatgcgaa tttgcgtgaa aaaatgggtg aaaccaaaga aagaaatcgt
    18541 cttgcccgtg aaattcatga tacattaggg catactttaa cgggattaag tgttggtctt
    18601 gaggcttgtc aggtgatgat tgatagggat gtcaatgtca caaaagcaca attgggtatt
    18661 ttgcaagaga gcgcaaaacg aggcttaaca gatgtcaggc gttcagtgga taaattgaag
    18721 ccagatgcgt tagaacgcta ttcattaaaa gaagctttag atatgatgat catggacttt
    18781 caaaaattga cggatgtaac gattctttat ttgtgtcatt tgcctttggt gaatttaaat
    18841 gcagatgaag aagaaattgt gtatcgtgta gtgcaagaag ggatgaccaa ttctgtaagg
    18901 catggtcatg cgacaaaaat ttatgtttcg attgcgcaag ccgataataa tttgattata
    18961 attattgaag ataatggtca aggatgtaag gatgtaaatc cgggctttgg tattcatcat
    19021 ttgacggaac gtattaattt attacaggga cgaattcgtt attatggcga taatggattt
    19081 gagttaattg tagaaatacc aatgagaaga ggagagaata atggttaaaa ttatgattgc
    19141 ggatgatcag cagctcattc gagaaagctt aaagattatt ttagattcgg atccaagttt
    19201 tgaagttgta aaagtggttg gtagtgggca agaagtatta gattcattaa aaacagaaag
    19261 tgtagatatt atcttgatgg atgtgcgtat gccaaatatg agtggtgtgg attgtacgcg
    19321 aattgtgaag aaagaatatc caaatataaa agttataata ttaacaacat ttgatgatga
    19381 tgaatatatt tatgatgcct taaaatatgg tgcaagtggt tatcttttaa agggttgttc
    19441 cttagaagaa ttgagttcag ctattcatag tgtattatct gggggagctt gttttaatcc
    19501 gaatgtggca ggaaaagttg cacagttctt ttcgcaaatg gcaaactcga attcggaatt
    19561 tagtcttgag catgaagaag attttgactt gagtgataca gaacgacaaa ttattgcaca
    19621 aattggacat ggctattcga ataaagaaat tgctcaaaaa cttggattta gtgagggtac
    19681 gattcgcaac tatttaagtg tgattttgga taaattggat ttaagagatc gcacacaatt
    19741 ggctatttgg tatttgtcta ggggaaataa ataatggtga gaaaaagatt aagaactttt
    19801 ttgatagcca tattggtgtg ttgtttggca gctacatttt attatacaca aaaacccatt
    19861 gttttaacga taggtgtatt tgcgggtagt aactggaatg tgccaagtcc atacagtggc
    19921 aaaattattg ataatgtgat tgaacgattt gagaaaacac atcccaatgt gcaagtggaa
    19981 tatgtgagtg gaatcttaaa agaggattat tcagattggg tttcaaagga agctttaaat
    20041 ggtaaattgc ccgatgtgtt tatggtgttg agtgatgatc tttctacata tgctaaggtg
    20101 ggaatgttag aaccattaga tacgtttatg cagacggatg cagactttaa taaaaatcgc
    20161 tatttttcaa caacattaaa tgcaggaaat atatatgatc atcaatatgc acttccatat
    20221 gaaagttcac caactttgat gtttgtgaat aaaacgttat tagaagaaaa tgggattgca
    20281 atcccaaatg tggattggac gtgggatgat ttttattcaa tttgtgaaaa attgactgtg
    20341 gatacggatg gtgatggaga aatcgatcaa tttggagagt atggctatac ttggcgaaat
    20401 gccctaagtt cgaatggaca ggcgttgtat gatgaagaaa gtatgaagtg tactttgaag
    20461 aaacaggatg tgtattcagc tattgaattt gtgcgtcaat taaatcgttt aaatcgaaat
    20521 cagaatgtga caagtgaatt gtttgataaa ggcaagattg ccttttgtcc aatgatgttt
    20581 agtgagtata gaacatataa accgtatcct tggagcgtta aaaaatattc aagttttgaa
    20641 tgggattgtc ttccaatgcc tgcaggacct agtggcaata atgtatcgca aatggatagt
    20701 ttactttgtg gaatcagtaa aatgtcttct aagaaaaaaa tggcttggga atttttgaag
    20761 ttactttgtt atgatgagac aacgcaagag agtttgttta aatattcgca gggtgtctcc
    20821 gtgttgaaga atgtgtcgac atccaaaaat gtgactaagt atattcaaga ggatgcgcca
    20881 ttagattcaa attataattt agattttttt gatgatatga tggaacaagc aatcacgata
    20941 aaaaaactgg atggcaatga tcaattgtat tcgatggcag atagtgaaat tcgaagagtc
    21001 attgatacaa atgaggatat tgagttggca atgcaaaatt tgaatgatga attaaatata
    21061 ttattggaga aacagtcctt aactttgtaa aaaatgtata actacaattg aatttttaaa
    21121 tgaatagttg aaatttcctg tatttatgga cgatttcaac ttttttgttg gtggaa
//
LOCUS       DBMDWL010000014        20737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_920_length_20737_cov_3.071574, whole genome shotgun sequence.
ACCESSION   DBMDWL010000014 DBMDWL010000000
VERSION     DBMDWL010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 20737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20737
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_920_length_20737_cov_3.071574"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(146..961)
                     /locus_tag="ACZPDN_01815"
     CDS             complement(146..961)
                     /locus_tag="ACZPDN_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HIR1297393.1"
                     /translation="MKKIYISADIEGIWGNSNPANTAKNGYLYSEYCTNMMNEVNLVI
                     DLLFQNGVEEVVVNDSHGNMDNLLPSKLDPRAAFVAANGAYKEFGMMEGLDDSFDGAM
                     FIGYHSRCNSPGVMAHTIWGTMVRKIVVDGKELGESGINRLLADEFGVPVILASGDSE
                     YGKQVEEELRCVFVETKKTLNNQCALCCSFNELKNRYTKGIQEALKVDEKPTLSSSHT
                     MDITFHHSRNADFVSRMDNVEKMDDCTVRINKETYKDLYALMRFVIKVCNAYA"
     gene            complement(963..2135)
                     /gene="hydF"
                     /locus_tag="ACZPDN_01820"
     CDS             complement(963..2135)
                     /gene="hydF"
                     /locus_tag="ACZPDN_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HIR1297394.1"
                     /translation="MSNLNSTVSANRTHIGFFGLRNAGKSSVVNAITNQSMSLVSNIK
                     GTTTDPVKKSMEILPIGPVVIVDTPGMDDEGTLGELRIKRTLEVLKTIDIAVLVVDAN
                     VGLQEVDLDLIGMFKENKLPYFIAFNKCDLIEEKKELASNEIYVSALKNVNIDGLKHM
                     LASSTKKQEEKTIIKDLIKTGDVVVLAIPIDKSAPKGRLILPQQLVLRDILDHHAIGM
                     TCQVEELEETLKRVTPKLVITDSQAFNKVSQIVDKSIHLTSFSILMARLKGSLAIVLE
                     GANKLDHLQDGDCILISEGCTHHRQCGDIGTDKLPKWILNYTKKNVTFEFTSGHGFKE
                     DLSKYSVVIHCGACMLNEKEVQSRVKECKKQGVPFTNYGIAIAKMNGILDRSVEVF"
     gene            complement(2137..3585)
                     /gene="hydG"
                     /locus_tag="ACZPDN_01825"
     CDS             complement(2137..3585)
                     /gene="hydG"
                     /locus_tag="ACZPDN_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HIR1297395.1"
                     /translation="MSIYNPKSLKAEEFINHEEILETIEYAEKNKRNVELIDSILNKA
                     RPQKTEHGVHCEGLTHREASVLLACDIPEKVEEMYKLANEIKQAFYGNRIVMFAPLYL
                     SNYCVNGCVYCPYHMKNKHIKRIKLTQDQIRDEVIALQDMGHKRLAIEAGEDPVHNSI
                     EYILESIKTIYSIQHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYNKESY
                     LELHPTGPKHDYDYHTEAMDRAMEGGIDDVGLGVLFGLEGYQYEFAGLMMHAEHLEAV
                     HGVGPHTISVPRVKHADDIDPEAFDNSLDDEIFEKIAACIRIAVPYTGMIISTRESQE
                     VRGKMLHLGVSQISGASRTSVGGYTEEDRPHDSEQFDVSDQRTLDEVVNWLMQLGYIP
                     SFCTACYREGRTGDRFMSLCKTGQIQNCCHPNALMTLCEYLVDYASEDTKKIGFELIE
                     KELNKIPNAKAQQIARENVAAIKSSNRRDFRF"
     gene            complement(3602..4645)
                     /gene="hydE"
                     /locus_tag="ACZPDN_01830"
     CDS             complement(3602..4645)
                     /gene="hydE"
                     /locus_tag="ACZPDN_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HIR1297396.1"
                     /translation="MIEAYRLIDKLEREHALSLLEYQTLIEVENEALRLYASNKAKEV
                     ANSIYGNSVYVRGLIEVGNYCRNDCFYCGIRKSNSNCDRYILDEEDILNCCKEGYKLG
                     FRTFVLQGGEGIYKKDFVTNIVQKIRSEYPDCAITLSLGEEDRDTYKAWFDAGADRYL
                     LRHETANKSHYEKLHPKNMSFDHRIQCLKDLKEIGYQVGCGFMVGSPHQTSETLAMDL
                     KFIETFKPDMCGIGPFISQKDTPFKNMESGTLNQTLFLLSLIRLIQPNILLPATTALG
                     TIDPKGREKGILAGANVVMPNLSPVSVRKKYALYDNKICTGEESAECKDCLSKRMNSI
                     GYKIVTDRGDIKK"
     gene            complement(4638..4895)
                     /locus_tag="ACZPDN_01835"
     CDS             complement(4638..4895)
                     /locus_tag="ACZPDN_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HIR1297397.1"
                     /translation="METRVAVMGIIVEDTNAVNLLNEILHEYSEFIIGRMGIPYKQKH
                     ISVISIALDAPQNTIAALAGKLGNIKGISVKTAYSNVISHD"
     gene            5133..6572
                     /locus_tag="ACZPDN_01840"
     CDS             5133..6572
                     /locus_tag="ACZPDN_01840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1297398.1"
                     /translation="MKNKYFEQKNFVKNYIYAITIFIITLVIGISIFFNVVQASVEKN
                     SRETLITNVTQQSEHLNTILNINYSYLNVLAQELSKSEDLFSENNISLIKAFMENTDL
                     NHTAIIDSDGNALYDNNVVKNVAHRRYFKESMQGKQSLSDPLESSVDQQTRVILSVPI
                     YKNNQVIGVVGGSYNVTKLGNMLFDDLFNGQGKSFIVDQDGNLITRDKKYEKKHNIKT
                     IDNLFDICDEKEVKTDFNQQESDLIQIQTKKNKSLYLAYSPLKINDWMICYIVPVHVA
                     QESYTFIKHYETLLATFLGLIVLSLMIYLAHSNSRENKYLIHLSEIDPLTSVFNKETT
                     QKLIDQKLKNHEHFCFLILDVDDFKSVNDNYGHAIGDKVLKNLSDLFKNHFRQTDIVG
                     RIGGDEFIILIEDEHIAESRIQSLLKKVNELKIEELQDFKLSISVGMAFAPSNGTTFM
                     ELYRHADHALYQTKRTGKNNYKIYKNDEN"
     gene            complement(6586..7365)
                     /locus_tag="ACZPDN_01845"
     CDS             complement(6586..7365)
                     /locus_tag="ACZPDN_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297399.1"
                     /translation="MSTEMLMKNRERFIKNVNSNENGAAALSLRLFCYARFMAVKNPY
                     AETHDWVYENELTNAQTNQYVFRLLDESTERPSMDMLIRKLKSVRLVRDSEMNGRKIY
                     VIKFTDIEMEELMNKSIIDKPSDKLLKEKKSSVKKEKEEVVNSSWKEVFLKNSKGENL
                     QILRFWFYIQYNAEKNGKKYISKSQALSEGAIEYVYKGIPKKMDVLKDLFALMKQLKL
                     VKEDVVKNEIVIRPAKLEGIEIKDYIKDKPVDLNSFNYTNV"
     gene            complement(7453..9213)
                     /locus_tag="ACZPDN_01850"
     CDS             complement(7453..9213)
                     /locus_tag="ACZPDN_01850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR1297400.1"
                     /translation="MNSEWSLDVLYKGFDDPKFSEDLISFEKKAKELDEFSKTLSHEN
                     EKEDLLKIVKGLEEFELLEKHLSCFGFLSQSTNTANAKAAAAIDKVATIASTASKAMA
                     IFSRYIADMKDLNSYLDCPELCEYKYFLNDIHESGKYLLSEDVEEALAKMNISGGSAW
                     EKQQSLLTSSLEVEYDGKKLPLASVRNLAYDADKEVRKAAYEAEIKSYAPIADAVSFS
                     LNNIKLQVITEAKMRGYTSPMDMTLHQSHVSQKTLDALLSAMQKYLPVFHKYLKRKAE
                     ILGYENGLPWYELFAPVGKSATTSFTPETTKEYLVNHFRPFCDELADMMEEAFDNNWI
                     DFYPHKGKVGGAFCCNMPFVKQSRVLTNFDGGLGDVVTLAHELGHAYHGLNIESHKPL
                     NWEYSMPVAETASTFNENIIMNAVIDETEDKEVKLGLIENQLQDLCQIICDIYSRYLF
                     EKAVFDRRSDEFLFTDQLNEIMLDAQKQAYGDGLDPTCLHPYMWVCKSHYYSSDLSYY
                     NWPYAFGGLFARGLIVKYQEMGKEEFVPKYKEMLHTTTIASVEDTASVLGIDLTDEAF
                     WISALETAKLRIEEFIELSK"
     gene            complement(9274..10008)
                     /locus_tag="ACZPDN_01855"
     CDS             complement(9274..10008)
                     /locus_tag="ACZPDN_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1297401.1"
                     /translation="MTKELIVVKDLHKNFGDLEVLKGIDQTIHQGEVVSIIGPSGSGK
                     STFLRCLNLLEKPTSGQIIFEGVDIAAENVDIDTHRKKIGMIFQHFNVFPHLTVLENI
                     TITPILELKTPKAEAEKEAIELLKKVGLEDKKDEYPRKLSGGQKQRLAIVRALAMHPD
                     VILCDEPTSALDPEMVKGVLEVIKELANQGMTIVIVTHEMGFARETSDRVLFMDEGII
                     QEQGTPEDIFEHPQNPRTIKFLSQVL"
     gene            complement(10001..11530)
                     /locus_tag="ACZPDN_01860"
     CDS             complement(10001..11530)
                     /locus_tag="ACZPDN_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIR1297402.1"
                     /translation="MKKGFKLVSTFLVSFMIIMSSIMPAFADETDTPTTGDLLDKIQE
                     RGELVVGTSPDFPPNEFIDSTKTGQAQYVGSDIELAKYIAKKMGVKLTIKASSFDTVL
                     ANLQTEEIDLAITGLAYTPARAQQMEMSIGYNMTEDTGGQAVIISKKDEEKYKKLTDL
                     TGLKVAAQQGSIQQQYTEDQIPDAKLQSISSIDDGVALLKNGTVQAVACSEGTADEYI
                     EKNSELAKLDELFNIDQDYAGNRVGAPLGEKRLMKEVNKILKEVNKKGLYEKWYKAAK
                     KQSSEQFTLTATGFFGTCVQIVQYCWPQLLKGLGVTLGLAAITVIFGTIIGGLFALVK
                     LSKNKIVQAIAGVYVELIRGTPLLLQLWLFINIFSYLTNGNMPMIVSVIIALIINSSA
                     YVAEIIRSGIQSVDKGQREAAKSLGMSNVHMMTKIIIPQAIKNILPALGNEFVMMIKE
                     TSLASTFYIGELMTVNSVIKSATYKSIEPLVIVGLIYFIVTYSLSKLIKYMEGKLSVS
                     D"
     misc_binding    complement(11573..11781)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11833..12789)
                     /locus_tag="ACZPDN_01865"
     CDS             complement(11833..12789)
                     /locus_tag="ACZPDN_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HIR1297403.1"
                     /translation="MRSANLLNDFAFKYVFGEDCKEANDALKSLLTVFLERKVHHVVV
                     KNSEMVKDYTKMKSPRLDLLVEFDDKTTVDLEMQLRQTKDNLLNRFNYYLARLHGSQD
                     MEGKSYSQLKETIVMVFFNLIIADDDDICSLYTMRKYSGIPLVKDEKEDRMKLMTIEM
                     PKVDLNKPLEDMNEQEKMIYYFLNCHKGLDDSKIKVMIESDEVIQMLEKRVETISDDR
                     WKKIIEDFQKLHENEERIELQLELEEAQRQMKEARKDKEEARKEKEEAHKEKEEARKA
                     ILEADKKVEQMVRKMHESGMSLEQISAYCSLNVNEIELLIKS"
     gene            complement(12885..13229)
                     /locus_tag="ACZPDN_01870"
     CDS             complement(12885..13229)
                     /locus_tag="ACZPDN_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR1297404.1"
                     /translation="MHELGIVVHISKTLEELAQENNITDIRRVTLQIGKVSGIVPEYL
                     VDCWKYYRKKVPLIENSSLEYEWIEAITICNECEKTYDTIQYGRKCPYCKSEHTVLLQ
                     GNECLIKEIEAE"
     gene            complement(13231..15912)
                     /locus_tag="ACZPDN_01875"
     CDS             complement(13231..15912)
                     /locus_tag="ACZPDN_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1297405.1"
                     /translation="MAYRDNIDKLARRITDRIPAKVKKLSQTDPEYECLNEILNDEQC
                     DLCLCFEVRKPLSFEEVVKRSGWPAGRVNRVLNEVCDIGMIEYNWENADHHKQFVLPQ
                     FVPGAAEFMVMNKDLVEEHPIVADFFEKMTRLPLEKVTPMVPPGGAGIGMHVIPVEKA
                     IHANQEAVSVEKISHWLNKYDKYALSPCSCRRQQRVRGEGTGDVEADVCIAVGDMADY
                     VVQTNKGGHYCTYEEVIETLERCERKGYVHQITNIDGEDKIFAICNCAHGVCNALRTS
                     QLFNTPNMSASAYRAHVDSEKCVACGKCVEICLVGAAKLGQKLCTKDGPVSYPKQELP
                     DDVKWGPEKWNWNFRDTAKVNCYETGTSPCKSACPAHLPVQGYIKMAKEGRYMDALKL
                     IKDFNPFPAVCGAICNRRCEQQCTRGLIDEPLAIDEIKKFIAEQELHEDKRYIPLCEN
                     VEGKFFTNKMAIIGAGPAGMSVAYYLRIMGYPVTIFEKEEKPGGMLVYGIPSFRLEKS
                     VIEAEIEVLKAMGIEFKCGIEVGKDISLDDLRNEGYEAFYVAIGAQGSRKLNIPGEEN
                     EMVESGIDFLRKVNKTEDPNLISGDVVVVGGGNVAIDVARAALRSGAEKVHLYSLEQE
                     AEMPASFEEVQEAKEEGIILHCGWGPKEIVEDSIVFKQCVSVKDETARFNPSYDESVL
                     ETIQCSHVLLSIGQSIIPIQGFDVEVNPNGTIKADGTTLQTSVEDVFVGGDVYTGPKF
                     AIDAISHGKEAAESMHRFVHKGHSLTIGRDLHLFNEFDKNNALIDIDFDHAKRQIPGI
                     KKGLGEKSFRDLRSAFTEEQVKIEANRCLGCGASIVDTNKCIGCGLCTTKCEFDAIHL
                     TRDIKDGARMVKAEDKMKAILPYAAKRQIKIIRNKKK"
     gene            complement(15914..16576)
                     /gene="hypB"
                     /locus_tag="ACZPDN_01880"
     CDS             complement(15914..16576)
                     /gene="hypB"
                     /locus_tag="ACZPDN_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIR1297406.1"
                     /translation="MYKIIEVKQSVFEDNNKDANKLREECKDKGVFLLNVMSSPGAGK
                     TTTLSRTLERLKDRMRIGIMEADIDSDVDAKTISEYGVRTIQLHTGGMCHLDADMTRQ
                     GLHEMGMEDLDLAVLENVGNLVCPAEFDTGSTKNVAILSVPEGDDKPLKYPLMFQVCD
                     VVLINKMDVLPYFDFDVEKCKENILYRNPNAKIFEVCAKTGERIDDWCKWLENEVKAW
                     KE"
     gene            16701..17489
                     /locus_tag="ACZPDN_01885"
     CDS             16701..17489
                     /locus_tag="ACZPDN_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012993.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR1297407.1"
                     /translation="MYQMSVSIDNQQNIAKLKDIPDCDILVLPEMWNCPYHNDALKTA
                     YLRHKESLMALKKLAKSHRVWIVAGSICADKYNRCYILNSDGDIVCSYDKTHLFEFHN
                     KQDYCENEVFVPGNHLQCFDTPWGRCGILLCYDIRFPEVSRILAQNGAQILFCPAAFN
                     EQATKKHWKLFVQTRALENEVFLCGVAPAKYTYKNYTSGGHSMLVDGFGNIVVELDEE
                     EACKVVDIHLKDIEKVRKRMPYWKVRRNDLYELKEIKNEDNSNQ"
     gene            17467..18435
                     /locus_tag="ACZPDN_01890"
     CDS             17467..18435
                     /locus_tag="ACZPDN_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR1297408.1"
                     /translation="MKTIQINEIEGFQIGSAQDTENATGCTVILCKEGATAGVDVRGG
                     GPATRETDLLNPKNMVQKIHAVTLSGGSAFGLESSSGVMQYLSEHEIGFDMKNIYIPI
                     VCEACLFDCGVGNSKAYPNKQMGYDACMEAEKNDPKQGNVGAGTGASVGKFFGPQYAM
                     KAGLGFSALQIGPLKVGAIVAVNACGDIFYPNSDKPIAGIYDRNTNTRLFSEDEILKA
                     AEKMINSCGMNTTIGCIITNADLNKAQMNKIASMAHNGYARCIRPVHTSSDGDTIFAM
                     TSNKVPAEQDLVGIMAVKAMEQAIVNAGTLADSAYGLASYKEITKN"
     gene            complement(18525..19220)
                     /locus_tag="ACZPDN_01895"
     CDS             complement(18525..19220)
                     /locus_tag="ACZPDN_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297409.1"
                     /translation="MLRKIIQIDEDKCTGCGICVDACHEGAIGLVNGKAKLMRDDYCD
                     GLGDCLPTCPTGAISFIEKDTLAYDEEAVKKNMEKRMKESGTMHSGCPGSMMRTLNPV
                     VSSPSIDVSSQLTSWPVQIKLAPINAPFFQHAKLLIAADCTAYAYANFHQEFMKNKVT
                     LIGCPKLDGVDYSEKLCDIIRMNSIESVTIVRMEVPCCSGLENAVKKALQESKKFIPW
                     QVVTISIDGKILD"
     gene            19305..19859
                     /locus_tag="ACZPDN_01900"
     CDS             19305..19859
                     /locus_tag="ACZPDN_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1297410.1"
                     /translation="MDSTFKQYKKNEIIYHMEDIPQAIGIVKEGSVVIETVDFLGNVS
                     LLSHIGPNQMFAESYALTKTPMYVYVRAVEDCTIQFLDVQALEKSPELKDQMIQILSN
                     KNKMLSQHIFHISNKTIRNKVLSYLSFMKLETQNSTFKIPFDRQQMADYLNVERSALS
                     KELSKMKSEGLIDYHKNTFTVYSI"
     gene            complement(19852..20643)
                     /locus_tag="ACZPDN_01905"
     CDS             complement(19852..20643)
                     /locus_tag="ACZPDN_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR1297411.1"
                     /translation="MKQNLHTHSIFCDGKDMIEEMTLEAISKGFDILGFSGHGYLDVD
                     TSSMRDTSGYISEVERIKSKYASSIQIHLGIEEDMLGRISEKAPFEYVIGSKHFLGET
                     AVDYSKPVFDSLFESYSGDYKKMCKDYYSDLSNMYDWQEVDIIGHLDLITKYNEDESY
                     FQFNDKDYMNIVCDCIDRLLINDKIFEVNTGAIARGYRKTPYPSKNILEYLYAKNARI
                     CLNSDCHDKNYLDCAFEESLDLIQSCGFKELQILTKDGFVPVKIK"
BASE COUNT         6562 a         4197 c         2871 g         7107 t
ORIGIN      
        1 aacatagaat gatattctat aagtgcctta aagataaggt aatgaatata gaaataaacc
       61 aatattcata tctctctttt aaaaataata ataaacaaaa aggacccatt ttaagacacc
      121 aacacttaaa atgggtcctt tgtttttaag catatgcatt acaaacttta atcacaaaac
      181 gcatcaatgc atataaatct ttatacgttt ccttatttat acgaactgta caatcatcca
      241 tcttttctac attatccatt cgagatacaa aatccgcatt acgactgtga tggaaagtaa
      301 tatccattgt atgagatgaa gacaatgttg gtttctcatc tacttttaaa gcttcttgaa
      361 tacccttagt atatctattc ttcaactcgt taaagctgca acataaagca cattgattat
      421 tcaatgtctt ctttgtttct acaaagacac aacgaagttc ttcttcgact tgtttaccat
      481 attcactatc accacttgct aagataacag gtacaccaaa ttcatctgct aataatcgat
      541 ttatgccact ttcaccaagt tcttttccat ctacgacaat ttttcgtacc atcgtacccc
      601 aaattgtatg agccattaca ccaggcgagt tacaacgaga atgataaccg ataaacattg
      661 caccatcaaa agaatcatct aacccttcca tcatgccaaa ttctttataa gctccatttg
      721 ccgctacaaa agcagctcta ggatctaatt ttgaaggaag aagattatcc atatttccgt
      781 gactatcatt cactacaact tcttctacac cattttgaaa caataaatca atcacaagat
      841 ttacttcatt catcatattt gtacaatatt cagaatataa atatccattt ttagctgtgt
      901 ttgccggatt actattcccc caaatacctt caatatcggc tgatatatat atttttttca
      961 tcttaaaaca cctctacaga acgatctaaa atcccgttca tttttgcaat cgcaatgcca
     1021 taatttgtga aaggaacacc ctgtttcttg cactctttca ctctagactg tacttctttt
     1081 tcatttaaca tacatgcacc acaatgaatc acaacactat attttgaaag atcttcctta
     1141 aatccatgtc ctgaagtaaa ttcaaaagtt acattcttct ttgtataatt caaaatccat
     1201 tttggaagct tatctgtgcc aatatcacca cactgtctat ggtgcgtaca accttcacta
     1261 atcaatatac aatctccatc ttgtagatga tctagcttgt ttgccccttc aagaactata
     1321 gctaaacttc cttttaaacg tgccataaga atggagaaag aagttaaatg aatcgattta
     1381 tccacaatct gactaacctt attaaaagct tgagaatccg taataactaa ttttggagtc
     1441 actcttttta atgtttcttc aagctcttca acctgacatg tcataccaat cgcatgatga
     1501 tccaagatat ctcttaacac aagttgttgt ggtaaaatca atcttccctt aggtgctgac
     1561 ttatcaatcg gaatcgcaag cacaacaaca tcacctgttt taattaaatc tttaatgatt
     1621 gtcttttctt cctgtttctt tgtacttgat gccaacatat gttttaaccc atctatattt
     1681 acattcttta atgcactcac atagatttca ttagaagcca actctttctt ttcttcaatc
     1741 aaatcacact tattaaaagc tataaagtaa ggtaatttat tttctttaaa catgccaatc
     1801 aaatccaaat ctacttcttg taaacccaca tttgcgtcca caaccaatac agctatatca
     1861 attgttttta aaacttctaa tgttcttttg attcttaact cacccaaagt tccttcatca
     1921 tccatacccg gtgtatccac aattacaaca ggtccaatcg gtaagatttc catagatttc
     1981 tttacaggat ctgtcgtagt tccctttata ttagaaacta atgacatact ctggttagta
     2041 atcgcattga cgacactgga ttttccagca tttcttaaac cgaaaaatcc aatgtgtgtg
     2101 cgattggcac ttactgtact atttaaatta gacatattag aacctaaaat ctctacgatt
     2161 actagatttg attgcagcta cattttcacg agcaatttgt tgagctttcg catttggaat
     2221 cttatttaat tctttttcaa tcaattcaaa accaatcttc ttcgtatctt cactcgcata
     2281 gtcaactaaa tattcacata aagtcatcaa tgcgtttgga tgacaacaat tttggatttg
     2341 tcctgtctta cacaaagaca tgaaacgatc tcctgttctt ccttctctat aacatgcagt
     2401 acagaaagaa ggtatgtagc caagctgcat caaccaattt acaacttcat ccaatgttct
     2461 ttgatccgac acatcaaatt gttctgaatc atgaggacga tcttcttctg tatatccacc
     2521 aacagatgta cgacttgctc cactaatttg agaaacacct aaatgaagca tttttccacg
     2581 aacttcttga gactcacgag ttgaaataat cattcctgta tatggaacgg caatacggat
     2641 acatgccgca attttttcaa aaatttcatc atccaaacta ttatcaaatg cttctggatc
     2701 aatatcatct gcatgtttta cacgaggcac actaattgta tgtggaccta caccatgaac
     2761 agcttctaag tgttctgcat gcatcatcaa cccggcaaat tcatattgat agccctctaa
     2821 accaaacaat acgcctaaac caacatcatc aattccacct tccatggctc tatccatagc
     2881 ttctgtatga taatcataat catgtttagg tcctgttgga tgcaactcta aataactttc
     2941 cttattatat gtttcctgaa ataagatata tgttccgatt ccagcttctt ttaacttacg
     3001 atagttttca accgttgtag ctgcaatatt tacattgaca cggcgaatcg caccattttt
     3061 atgttgaatt gaataaatag ttttaataga ttctaaaata tattcaattg agttatgaac
     3121 tgggtcttcg cctgcttcaa tggccaaacg tttatgtccc atatcctgca aagcaataac
     3181 ttcatcacga atctgatctt gggttaattt aatacgcttg atatgtttat ttttcatatg
     3241 ataaggacaa tatacacatc cattcacaca atagtttgat aaatataatg gggcaaacat
     3301 cacaatacga ttgccataaa atgcttgttt gatttcattg gcaagcttat acatttcctc
     3361 tactttttca ggaatatcac aagctaaaag tacacttgct tcacgatgtg tcaagccttc
     3421 acaatgaacg ccatgctccg tcttttgagg acgagcctta tttaaaattg aatcaatcaa
     3481 ttctacattg cgcttgtttt tttcagcata ttcaatcgtt tcaagaatct cctcatgatt
     3541 aataaattct tctgccttta atgattttgg attataaata ctcatttttg tttctcctct
     3601 tttatttttt tatatctcca cgatctgtta caattttgta accaatcgaa ttcatacgtt
     3661 ttgataaaca atccttgcat tctgcacttt cttcacccgt acaaatttta ttatcataca
     3721 acgcatattt ttttctaaca cttactggtg ataaattggg cataacaaca ttggccccgg
     3781 caagaatccc tttttctctt ccctttggat caattgttcc aagtgctgtt gtggctggaa
     3841 gtaagatatt tggttgaatc aaacgaatta aagataataa gaataacgtt tgattcaaag
     3901 taccactttc catattttta aaaggcgtat ccttttgtga aataaatgga cctattccac
     3961 acatatcagg tttaaatgtt tcaataaact tcaaatccat cgccaaagtt tcacttgtct
     4021 gatggggaga acccaccata aagccacagc ctacttgata gccaatttcc tttaagtctt
     4081 ttaaacactg tatgcgatga tcaaaagaca tattctttgg atgcagcttt tcataatgac
     4141 ttttatttgc ggtttcatgt cttaataaat agcgatccgc tcctgcatca aaccaagcct
     4201 tatatgtatc tctatcttct tcacctagag aaagggtaat tgcacaatct ggatattcac
     4261 tacgaatctt ttgaactata tttgtaacaa aatccttttt ataaatacct tccccacctt
     4321 gaagcacaaa agtgcgaaag ccaagtttat atccttcttt gcagcaattc aatatatctt
     4381 cttcatctaa tatatatcga tcgcaattgg aatttgattt tcgaatacca caatagaaac
     4441 agtcattacg acaatagttt cctacttcaa tcaagccacg tacatagact gaattcccat
     4501 atatagaatt tgcgacttcc ttcgctttgt ttgaagcata taatcgtaaa gcttcatttt
     4561 cgacttcgat taaagtttga tattccaata aacttaaagc gtgttctctt tcaagtttat
     4621 caatcaatcg atatgcctca atcatgacta atcacattgg aataggcagt tttcacacta
     4681 attcccttta tatttcctaa tttaccggcc aaagcagcaa ttgtattttg tggggcatct
     4741 aacgcaatcg aaatgacact aatatgtttc tgtttataag gaatacccat tcttccaatt
     4801 ataaactcac tatattcatg taaaatttca tttaaaagat ttactgcatt tgtatcttct
     4861 acaatgattc ccattacggc tacacgtgtt tccatattca gctccttcca aaagaaaagc
     4921 tatactccac acaaaaagag catagctata gttatactta caaattctca taagcacctt
     4981 tcacgcagaa ttctcttaat gttgggagca aatttcttta tatcttatat aatacactaa
     5041 aatgtgattg atttcacaaa ttatggttag tcttatataa tttgatattc ctttaccatt
     5101 tttcttggta taattagaaa aaggagttcc atatgaaaaa taaatatttc gaacaaaaaa
     5161 attttgttaa aaactatatt tacgcaatca ccatttttat cataactctt gtgattggaa
     5221 tttctatatt ttttaatgtg gtgcaagctt ctgttgaaaa aaattcaaga gaaacattaa
     5281 ttaccaatgt gactcaacaa agcgagcact taaatacaat tttaaatatc aactattcct
     5341 atttaaatgt actagcacaa gaattatcaa agtctgaaga cctattttca gaaaataata
     5401 tatctttaat taaagccttc atggaaaaca cagacttaaa tcataccgca atcatagatt
     5461 cagatggaaa tgcactatat gataataatg tagttaaaaa tgttgcacat cgaagatatt
     5521 ttaaagaatc gatgcaaggc aaacaatctc tttcagaccc cttagaaagt agtgttgatc
     5581 aacaaacacg cgttatttta agtgttccta tttataagaa caatcaagtc ataggtgttg
     5641 tgggcggttc ttataatgtt acaaaactag gaaatatgtt gtttgatgat ttgtttaatg
     5701 gacaaggaaa aagttttatt gttgatcaag atggaaatct aatcactaga gacaagaaat
     5761 acgaaaaaaa gcacaatata aaaacaatag ataatttatt tgatatttgt gatgaaaagg
     5821 aagtgaaaac agacttcaac caacaagaat cagatttaat ccaaatacaa accaaaaaga
     5881 ataaatcgtt atatttagct tattccccat taaaaatcaa tgattggatg atttgttaca
     5941 ttgttccagt tcatgttgct caagaatctt acacatttat taagcattat gaaactttgt
     6001 tggcaacatt tttaggcttg attgtactca gcttgatgat ttatttggct cactcaaatt
     6061 caagagaaaa taaatatttg attcatttat ctgaaattga cccattaact tccgttttca
     6121 acaaagaaac aacgcaaaag ctcattgatc aaaaacttaa aaatcatgag cacttctgtt
     6181 tcttgatttt ggatgtggat gattttaaat cagtgaatga taattatgga catgccatag
     6241 gtgataaagt attaaaaaat ttgagtgacc tatttaaaaa tcattttaga caaacggata
     6301 ttgttggaag aattggtggc gacgaattta tcattctaat cgaagatgaa catattgccg
     6361 aatcaagaat tcaatcttta ctaaaaaaag tcaatgaatt aaagattgaa gaactacaag
     6421 actttaaact ttccatcagt gttggaatgg cattcgcccc tagtaacggc actactttta
     6481 tggaattgta tcgccatgcc gatcatgcct tgtatcaaac aaaacgaaca ggcaaaaata
     6541 attataaaat atataaaaat gacgagaact aatctcgtca ttttcttata cattcgtata
     6601 gttaaaacta tttaaatcta ctggcttatc ctttatataa tccttaattt ctataccctc
     6661 taatttggct ggacgaatca ctatctcatt tttaacgaca tcttctttaa ctagttttaa
     6721 ttgtttcatc aatgcgaata aatcttttag aacatccatc tttttaggga ttcccttata
     6781 cacatattca atagcccctt cacttaatgc ctggcttttt gaaatatact tcttcccatt
     6841 cttttctgca ttatactgta tatagaacca gaatcgaagt atctgtaaat tttcaccttt
     6901 actatttttt aagaatacct ctttccaaga tgaattcacc acttcttctt tttctttctt
     6961 tacagaagac ttcttttctt ttaataactt atcgcttggt ttatcaataa tactcttatt
     7021 catcaattct tccatttcga tatctgtgaa tttaataaca taaatttttc tgccattcat
     7081 ttcactatca cgaaccaaac gaacactttt taatttacga atcaacatat ccatagatgg
     7141 tctttctgta ctttcatcca ataaacggaa tacatattga ttggtttggg catttgtcaa
     7201 ttcgttttca tatacccagt catgcgtttc tgcatacggg ttttttactg ccataaatct
     7261 agcataacaa aataatctta aagaaagtgc agctgcacca ttctcattac tatttacgtt
     7321 tttgataaaa cgttctctat ttttcattaa catttctgta ctcattaaat ctctccttga
     7381 aaaaattcga acgcaataga ttatatcatc atttgtgaac ttttgcagaa aaaattgctt
     7441 gtttccaagc aattatttcg ataattcgat aaattcttca attcttaatt ttgcggtttc
     7501 caatgcagaa atccagaaag cttcatccgt tagatcaata cctaatactg aagctgtatc
     7561 ttctacagaa gcaatggttg ttgtgtgtaa catctcttta tattttggaa caaattcttc
     7621 ttttcccatt tcttgatatt ttacaatcaa tccacgcgca aacaatccac caaaggcata
     7681 tggccagtta taatagctta aatctgaaga ataatagtgt gacttacata cccacatata
     7741 tggatgtaaa caagttggat ccagtccatc gccataagct tgtttttgtg cgtccaacat
     7801 aatttcattc aattgatctg taaataagaa ttcatcgctt ctacgatcaa atacggcttt
     7861 ttcaaataag taacgagaat aaatatcaca gataatctga catagatctt gaagttggtt
     7921 ttcaatcaag cctagtttta cttctttatc ttccgtttca tcaattactg cattcataat
     7981 gatattttca ttaaatgtac ttgccgtttc tgcaactggc atagaatatt cccaattcaa
     8041 cggtttgtga ctttcaatat tcaatccatg atatgcatgt ccaagttcat gagccagcgt
     8101 aacgacatct ccaagtccac catcaaagtt agttaatacg cgtgattgtt tcacaaatgg
     8161 catattacaa cagaaggctc cacccacttt tcctttgtgt ggataaaaat caatccagtt
     8221 attgtcaaaa gcttcttcca tcatatcggc caattcatca cagaatggtc taaaatgatt
     8281 tactaaatat tcttttgtag tttcaggggt aaaacttgta gtagctgatt ttccgactgg
     8341 cgcaaataat tcataccaag gaaggccatt ttcatatcca agtatttcag cttttctttt
     8401 caaatactta tggaatactg gcaagtattt ctgcattgca ctaagaagtg catctaatgt
     8461 tttctgggat acatgagact ggtgtagtgt catatccatt ggacttgtat atccacgcat
     8521 ttttgcttca gtgataacct gtagtttaat attattcaat gagaaactaa cggcatctgc
     8581 aataggtgca taacttttga tttctgcttc ataggcagct ttacgaactt ctttatctgc
     8641 atcatatgca aggttacgga cagatgccaa tggcaacttc ttaccatcat attccacttc
     8701 aagtgagctt gttaaaaggc tttgttgctt ctcccaagct gaaccaccag aaatattcat
     8761 tttagcaagt gcttcctcca cgtcttcact taacaaatat tttccacttt catgaatatc
     8821 atttaagaaa tatttgtatt cgcaaagttc tggacagtct aaataactgt tcaaatcttt
     8881 catatccgca atatagcgag aaaatatagc catagcctta ctcgctgtac ttgcgatggt
     8941 agctacttta tcaatggctg ctgccgcttt cgcatttgcg gtatttgtgg attggctcaa
     9001 aaatccaaag caactcaagt gtttttctaa taattcaaat tcttctaatc ctttaacaat
     9061 tttaagaaga tcttccttct cattttcgtg acttaaagtc tttgaaaatt catccaattc
     9121 ttttgccttt ttttcaaaag aaattagatc ttcactaaat ttaggatcat caaatccttt
     9181 gtacaatacg tctaatgacc attctgaatt catatcaatt tcctccaatt taataaatta
     9241 taccataaga aaagctcgag catatgccca agcttataat acttgtgata aaaatttaat
     9301 agttctagga ttttgtggat gttcaaaaat atcttctgga gtaccttgtt cttgaataat
     9361 accttcatcc ataaataata cgcgatcact tgtttcgcgg gcaaagccca tttcatgagt
     9421 tacaatcacg attgtcatcc cctggtttgc taattctttg attacttcta atacaccttt
     9481 taccatttct ggatctaatg cacttgttgg ttcatcacat agaatcacat ccggatgcat
     9541 tgctaaagca cgaacaatag ctagacgctg tttttgtcca ccagataatt ttcttggata
     9601 ttcatctttc ttatcttcta agccaacctt tttaagcaat tcaatcgctt ctttttcagc
     9661 ttctgccttt ggtgttttaa gttctaagat gggtgtaatc gtaatatttt ctaaaacagt
     9721 taagtgtggg aatacattaa agtgctggaa gatcatccca atctttttac ggtgtgtatc
     9781 aatatccaca ttttcagctg cgatatccac cccttcaaaa atgatttgtc cacttgttgg
     9841 tttttctaat aaatttagac aacgtaagaa agtagatttt cctgaaccac taggtccaat
     9901 aatactaaca acttctcctt gatggattgt ttgatcgatt ccttttaata cttctaaatc
     9961 tccaaaattt ttatgtaagt cttttacaac aattagttcc ttagtcactg acactcaact
    10021 tcccttccat gtatttaatt aattttgata aagaatatgt aacgataaag tagatcaaac
    10081 caacaattac taatggttca atagatttat aagtagcgga tttaattaca ctgttaacag
    10141 tcattaattc accaatatag aatgtacttg ccaaagatgt ttccttgatc atcataacga
    10201 attcattacc taatgcaggc aagatatttt tgatagcttg aggaataatg attttagtca
    10261 tcatatgtac atttgacata ccaagtgatt tagccgcttc tctttgacct ttatctaccg
    10321 actgaatacc tgaacggata atttcagcga catatgcact cgagttaata atcaacgcaa
    10381 tgattacact gacgatcatt ggcatatttc catttgtcaa ataagagaaa atattgatga
    10441 ataaccacaa ttgtagcaac aatggtgttc cacgaatcaa ttctacatat acaccggcaa
    10501 ttgcttgtac aatcttattc tttgataatt ttacaagagc aaataatcca ccaataattg
    10561 taccaaagat aacagttata gctgcaagtc ctaaagtaac tcctagacct tttaataatt
    10621 gtggccaaca atattgtaca atttgaacac atgttccaaa gaatccagtt gcagtcaatg
    10681 tgaattgttc actactttgt ttcttagctg ctttatacca tttttcatac aaaccttttt
    10741 tattaacttc ctttaagatt ttattgactt ccttcatcaa tcttttttcg cctaaaggag
    10801 cacctacacg gttacctgca taatcttgat caatattaaa taattcatct aacttcgcaa
    10861 gttcagagtt cttttcaata tattcatcag ctgtaccttc cgaacatgca acggcttgta
    10921 cagtcccatt ctttaatagg gcaacaccat catcaatact tgaaatcgat tgtagttttg
    10981 catctggaat ttgatcttcg gtatattgtt gctgaatcga tccttgttga gcagctactt
    11041 ttaagcccgt taaatctgtt aattttttat atttctcttc atctttttta gaaataataa
    11101 cagcctgtcc accagtgtct tctgtcatat tgtatccaat agacatttcc atttgttgag
    11161 cacgtgctgg tgtataagcc aatccagtaa tagctaaatc gatttcttca gtttgaaggt
    11221 ttgccaaaac agtatcgaaa cttgatgctt tgattgttag tttaacaccc atcttttttg
    11281 cgatatattt tgccaattca atatcagaac ctacatattg agcttgtcct gtctttgtcg
    11341 aatcaataaa ttcattagga gggaaatctg gagatgttcc cacaactaat tctcctcttt
    11401 cttgaatttt gtctaataaa tcacctgtag ttggtgtgtc tgtttcatcg gcaaaagcag
    11461 gcatgatact agacattata atcataaaac ttactaaaaa tgtcgaaact aatttaaatc
    11521 cttttttcat gttgtcctat tcctttctaa taaaaaaagc cgtccttgaa tatacaagga
    11581 cgactataaa atcgcggtac caccttattt cctgcacaaa agtacaggcc cttaactcat
    11641 aacgggagta tccgaagctt cctcaatctt cgaaagctta gctctaagac acgttcaccc
    11701 tgtgcttact gttccatttc caccatcagg aactcactat cagcagcatc cacgactact
    11761 tcttcttttc attgcctcta caaaggatgg ctttattata aacaaacatt tttttaattg
    11821 caataagaaa ttttatgatt ttatcaacaa ctcaatttca ttcacattta gtgaacaata
    11881 tgcagaaatt tgttcaagac tcattccaga ttcatgcatc ttacgaacca tctgttccac
    11941 tttcttatca gcttctagta tagcctttct tgcttcttct ttctctttat gagcttcttc
    12001 tttttcttta cgagcctctt ctttgtcttt acgagcttct ttcatctgtc tttgagcttc
    12061 ttcaagttca agctgtagtt caattcgttc ttcattttca tgcagctttt gaaaatcttc
    12121 tattattttc ttccaacgat catccgatat cgtttctaca cgcttctcca acatttgaat
    12181 cacctcatca ctttctatca tcaccttaat tttactatca tccaatcctt tatggcaatt
    12241 caaaaagtaa taaatcattt tctcctgttc attcatgtct tcaagtggtt tattcaaatc
    12301 cacttttggc atttctattg tcattaactt catacgatct tctttttcat ctttcactaa
    12361 aggaatgcca ctgtattttc tcattgtgta taaactacaa atgtcatcat catcagcaat
    12421 tatcaaatta aagaatacca tcacaatagt ttcctttaat tgactataac tttttccttc
    12481 catgtcttgt gaaccatgaa gtctagccaa ataataatta aatcgattga gtaaattatc
    12541 tttggtctgt cttaactgca tttctaaatc cacagttgtt ttatcatcaa attcaactaa
    12601 tagatccaac cgaggacttt tcattttagt atagtctttt accatttctg aatttttaac
    12661 aaccacatga tggactttcc tttctaaaaa taccgtaagt aatgatttta aggcatcatt
    12721 agcctcttta caatcttcgc caaacacata cttaaaggcg aaatcgttta ataaatttgc
    12781 gctacgcatg agcacctctt ttctagtagg catttcctac atactcatat acgtaaaaaa
    12841 agacactttt tcgaataaaa atcaaaaaaa gcgcctttcg tcacttattc ggcttcaatc
    12901 tccttaatta gacactcatt accttgaagt aaaactgtat gttcactctt acaatatgga
    12961 cattttcttc catattgaat cgtatcatat gttttttcac attcattaca aatcgtaata
    13021 gcttcaatcc actcatattc tagtgaagag ttttcaatta aaggaacctt cttcctataa
    13081 tatttccaac aatccactaa atactcagga acaattccac ttacttttcc aatctgtaaa
    13141 gtcactctac gaatatctgt aatattattt tcttgtgcaa gttcttctaa tgtttttgaa
    13201 atatgtacaa caatccctaa ttcatgcata ctatttcttc ttattacgaa tgatcttaat
    13261 ttgacgctta gccgcatatg gcaatatagc cttcatctta tcttccgcct taaccattct
    13321 tgcaccatct ttgatatcac gcgttaagtg gatcgcatca aattcacatt ttgtcgtaca
    13381 caagccacaa ccaatacact tatttgtatc gactatactt gcaccacatc ctagacatcg
    13441 attggcttca atcttaactt gttcttctgt aaatgcagaa cgtaagtctc taaatgattt
    13501 ttcacccaag ccctttttaa taccaggtat ctgacgtttg gcatgatcaa aatcaatatc
    13561 aatcaatgca ttatttttat caaattcatt aaacaagtgt aagtctcttc caattgtcaa
    13621 agaatgaccc ttatgaacaa aacgatgcat tgattctgct gcctctttac catggctaat
    13681 cgcatcaatc gcaaattttg gtcctgtata cacatcacca cccacaaata catcttcaac
    13741 agatgtttgt agtgttgttc catccgcttt gattgttcca tttggattga cttctacatc
    13801 gaatccttga attggaataa tactttgtcc aattgaaagt aatacatgac tacattgtat
    13861 agtttctaaa acactttcat catagcttgg attaaatcta gccgtttcat cctttacact
    13921 tacacattgt ttaaatacaa ttgaatcttc tacaatttct tttggacccc atccacaatg
    13981 aagaattata ccctcttctt ttgcctcttg tacttcctca aaacttgccg gcatttcagc
    14041 ttcttgttcc aaagaatata aatgtacctt ttcagctcca cttcttaaag ctgcacgagc
    14101 aacatctatc gcaacatttc cgccaccaac gacaaccaca tcaccagaaa ttagatttgg
    14161 atcttctgtt ttattcacct tacgcaagaa atcaattcca gactcaacca tttcattttc
    14221 ctcacctgga atatttaatt ttctagatcc ttgagcacca atggccacat agaatgcttc
    14281 atatccctca tttcttaaat catccaaaga aatatcttta cctacttcta tgccacactt
    14341 aaactcaatg cccatagctt ttaacacttc aatttcagcc tcaattacag acttttctaa
    14401 tctaaaagaa ggaataccat agactaacat accacctggt ttttcttcct tttcgaatat
    14461 tgtgacagga tagcccatta ttcttaaata ataagccaca ctcataccag caggacctgc
    14521 gccaataatg gccattttgt ttgtgaagaa tttgccttcg acattttcac ataaaggaat
    14581 ataacgctta tcttcatgaa gttcttgttc ggcaataaac ttcttgattt catcaatagc
    14641 caaaggctca tcaatcaatc cacgtgtaca ttgttgttca caccttctgt tacaaatagc
    14701 tccacatacg gctggaaatg gattaaagtc tttaattaac tttaatgcat ccatatatcg
    14761 accttctttg gccattttaa tatagccttg aaccggtaaa tgtgcaggac atgcactttt
    14821 acaaggcgat gttcccgttt cataacaatt gactttagct gtatctctaa agttccaatt
    14881 ccacttttca ggcccccact ttacatcatc tggaagttct tgtttaggat acgatacagg
    14941 gccatctttg gtacaaagct tctgacccaa ttttgccgca cccactagac aaatttctac
    15001 acattttcca cacgcaacac acttttcact atccacatgc gccctataag cacttgccga
    15061 catatttggc gtattgaaca attgacttgt tcttaatgcg ttacagacac catgtgcgca
    15121 gttacatatc gcaaaaattt tatcttctcc atcaatattg gtaatctgat gtacatatcc
    15181 ttttctttca caacgctcta aagtctcaat aacctcttca tacgtacaat aatgtccacc
    15241 cttgtttgtc tgtacaacat agtcagccat atctcctacc gcaatacaaa catcggcttc
    15301 aacatcgcca gttccttcac ccctaacacg ttgttgtctt ctacatgaac atggagaaag
    15361 tgcatattta tcgtatttat tcaaccaatg cgaaatcttt tctacactta ccgcttcttg
    15421 attagcatga atcgcctttt ctacaggaat gacatgcata ccaatgccag ctcctccagg
    15481 aggaaccatt ggcgttacct tttcaagtgg taatcgcgtc atcttttcaa agaaatccgc
    15541 aacaattgga tgttcttcaa caagatcttt attcataacc ataaattcag ctgctccagg
    15601 aacaaactga ggaagcacaa actgtttgtg gtgatccgca ttttcccaat tatattcaat
    15661 cattccaatg tcgcatactt catttaatac acgattcact cttcctgctg gccatccact
    15721 tcttttcact acttcttcaa aagaaagtgg tttacgtact tcaaaacaca aacataaatc
    15781 acattgttca tcatttaaga tttcatttag acactcatat tcaggatccg tttgggataa
    15841 tttttttact ttagccggaa tacgatctgt gatacgcctt gccagtttat caatattatc
    15901 tcgatatgcc atattattcc ttccaggctt ttacttcatt ttcaagccac ttacaccagt
    15961 catcaattct ctcacccgtt tttgcgcaca cctcaaaaat tttcgcattt ggattacggt
    16021 acaaaatatt ctctttacat ttctctacat caaaatcaaa gtatggcaat acatccatct
    16081 tattaattag aaccacatca cacacttgaa acatcaaagg atattttaaa ggtttgtcgt
    16141 ctccttctgg aacacttaaa atagccacat ttttagtgga tcccgtgtca aattcagcag
    16201 gacatactaa atttcctaca ttttcaagta cagccaaatc taaatcttcc atacccattt
    16261 catgcagtcc ttgacgagtc atatctgcgt ctaaatgaca cataccacct gtatgtagtt
    16321 gaatcgtgcg tacgccatat tcactgattg ttttggcatc tacatcacta tcaatatccg
    16381 cttccataat accaattctc attctatctt ttaatctttc taacgttcta gatagagttg
    16441 ttgtcttacc tgctccagga cttgacataa cattcaataa gaacacacct ttatctttac
    16501 attcttctct taatttattc gcatctttat tattatcttc aaatacactt tgttttactt
    16561 ctataatttt atacatgata caaaccctcc taaaatgatt gtaaacgatt tcaaatcagt
    16621 agtaaataaa cacatgttag tctttgcaca ccttgaaaag tagtcacttt tcgcctatca
    16681 taatctatat gaagatctgt atgtaccaaa tgtctgtttc aatcgataat caacaaaaca
    16741 tagctaaact aaaagacatt ccagactgtg atattttagt tctaccagaa atgtggaact
    16801 gtccctatca taatgatgca ttaaaaacag cttatttgcg tcataaagaa agtttgatgg
    16861 ctttaaaaaa actagctaaa tcgcataggg tatggatcgt ggctgggtca atttgtgcgg
    16921 ataaatacaa tcgttgttac atattaaata gcgatggtga cattgtttgt tcgtatgata
    16981 aaacacatct ttttgaattc cacaacaaac aagattattg tgaaaatgaa gtatttgtac
    17041 caggaaatca ccttcaatgt ttcgatacgc catggggaag atgtggcatt ttattatgtt
    17101 acgacatccg atttccagaa gtatccagaa ttctagctca aaatggagca caaattctat
    17161 tttgtccggc tgcttttaat gagcaggcca caaagaagca ttggaagtta tttgtccaaa
    17221 ctcgtgcttt agaaaacgaa gtctttttgt gtggtgttgc cccagctaag tatacatata
    17281 aaaactatac ttcaggaggc cattctatgc ttgtggatgg ttttggcaat attgtagtgg
    17341 agttggatga agaagaagcc tgcaaagttg tagatatcca tttaaaagac attgaaaaag
    17401 taaggaaaag aatgccttat tggaaggtgc gtagaaatga tttatatgaa ttaaaggaga
    17461 tcaaaaatga agacaattca aatcaatgag atcgaaggat ttcaaattgg tagtgcacaa
    17521 gatacagaaa atgcgactgg ctgtacagtc atcttatgta aagaaggagc tacagctggt
    17581 gtagatgtac gtggtggtgg tcctgctact cgtgaaacag atttgttgaa tccaaaaaac
    17641 atggtccaaa aaattcatgc ggtcacatta tcgggtggta gtgcttttgg tttagaatca
    17701 agcagtggtg taatgcagta tttgagtgaa cacgaaattg gttttgacat gaaaaacatc
    17761 tatattccca tcgtatgtga agcttgtcta tttgattgtg gtgtaggtaa ttctaaggca
    17821 tatccaaaca aacaaatggg atatgatgct tgtatggaag cggaaaaaaa tgatccaaaa
    17881 caaggaaatg tcggtgcagg aacaggggct agtgttggta aattctttgg ccctcaatat
    17941 gcgatgaagg ctggacttgg attttcggcc ctacaaattg gcccattaaa agtaggtgct
    18001 attgtagccg taaatgcgtg tggtgatatt ttctatccaa attcagacaa gccaatcgca
    18061 ggtatttatg atcgaaacac aaatacaaga ttgtttagtg aagatgaaat cttaaaggca
    18121 gctgaaaaga tgatcaattc ttgtggtatg aatacgacaa ttggttgtat catcacaaat
    18181 gcagacttaa ataaagccca aatgaataag attgcgagca tggcccataa tggatatgca
    18241 agatgtattc gtccagtcca cacttcaagt gatggagata ctatttttgc gatgacatct
    18301 aataaagttc ctgcagaaca agatttagtt ggaattatgg ccgttaaagc gatggaacaa
    18361 gctattgtca atgcgggtac acttgcagat agtgcttatg gtctagctag ctacaaagaa
    18421 atcactaaaa attaatcatt aaagtatcgc tacggtgata cttttttcat atcaaaaatg
    18481 acaaaagtgc aatatctgta acgataactg cactttttta agatttaatc taagattttt
    18541 ccatctattg aaattgtgac aacttgccaa ggaataaact ttttactttc ctgcaaagct
    18601 ttcttaaccg cattttcaag accactacag caaggtactt ccatacgaac aatggttaca
    18661 ctttcaatcg aattcatacg aataatatca caaagctttt ctgaataatc cacaccatca
    18721 agttttggac atcctattag agtcacttta tttttcataa attcctgatg gaaattggca
    18781 taggcataag ctgtacaatc cgcagctatt aaaagcttgg catgttgaaa gaagggtgcg
    18841 ttgataggtg ctagtttaat ttgaacaggc caagatgtta gttgacttga tacatcaatc
    18901 gatggacttg agacaactgg attcagtgta cgcatcatag atccaggaca tcctgaatgc
    18961 atggtgcccg attctttcat gcgtttttcc atattctttt ttaccgcctc ttcatcatag
    19021 gctaaagtat ctttttcaat gaatgaaata gcacctgtag gacatgtagg taaacaatcc
    19081 cctagtccat cacaataatc atctcgcatt aatttcgctt ttccattcac taagccaatg
    19141 gctccttcat gacaggcatc cacacagata ccgcatcctg tacatttatc ttcatcaatt
    19201 tgtataattt ttcttaacat aaaatcatcc tcatttctta tgaccttatt ttagtgtata
    19261 atacaatcaa tttatgttgt ttaaacaacg aaaggaaaat atttatggat tcaacattca
    19321 aacaatacaa gaaaaatgaa attatttatc atatggaaga tattcctcaa gccattggca
    19381 ttgtcaaaga aggaagtgtt gtcatagaaa ctgtagactt tttaggcaat gtttccctat
    19441 tatcacatat tggtcccaat caaatgtttg cggaaagcta tgccctaacc aaaacaccta
    19501 tgtatgtata tgttagagcg gttgaggatt gtacaattca atttttagat gttcaagcct
    19561 tagaaaaatc tccagaatta aaagatcaaa tgattcaaat actttcaaat aagaacaaaa
    19621 tgttgtcaca acacatattt catatttcca acaaaaccat tcgtaataag gtgttgtctt
    19681 acttatcgtt tatgaaacta gaaacacaaa attctacctt taagattccg tttgatcgtc
    19741 aacagatggc tgactattta aatgtagaac gaagtgcctt atcaaaagag ctttctaaaa
    19801 tgaaaagtga aggtctaatt gattatcaca agaacacctt cactgtttat tctatttaat
    19861 ttttactggt acaaaaccat cttttgtgag tatctgcagt tctttaaatc cacaagattg
    19921 aatcaagtct aaactttctt caaaagcaca atccaaatag ttcttatcat gacaatcact
    19981 gtttagacaa atacgtgcat tctttgcgta taaatattcc aatatattct tagatggata
    20041 tggtgtttta cgatagcctc tagcgatggc tccggtattc acttcaaaaa tcttatcgtt
    20101 aatcaacaat cggtcaatac aatcgcaaac aatattcata tagtctttat cattaaattg
    20161 aaaataagac tcgtcctcat tatatttggt aatcaaatcc aaatgtccaa tgatatccac
    20221 ttcttgccag tcatacatat tcgataaatc tgaatagtag tctttacaca tctttttata
    20281 gtcaccagaa taggattcaa ataaagaatc aaatacaggc tttgaataat ccacagcagt
    20341 ctcacctaaa aaatgtttac ttccaatcac atattcaaat ggagcttttt cagatattct
    20401 tcctaacata tcttcttcaa tgcctaaatg gatttgaata gacgaagcgt atttcgattt
    20461 aattctctca acctctgaaa tatatcctga agtatctcgc atggaacttg tatccacgtc
    20521 caaatatcca tggccagaaa atcctagaat atcaaagcct tttgagatgg cttctaaagt
    20581 catttcttca atcatatctt tgccatcaca aaagatagag tgtgtatgta gattctgttt
    20641 cataagtaaa tttacctcct tcttttactc atatttactt taaacagttg gagataaact
    20701 cctctaagaa ccgtacgtgc agttttcccg catacgg
//
LOCUS       DBMDWL010000015        20223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_946_length_20223_cov_5.028874, whole genome shotgun sequence.
ACCESSION   DBMDWL010000015 DBMDWL010000000
VERSION     DBMDWL010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 20223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20223
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_946_length_20223_cov_5.028874"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="ACZPDN_01910"
     CDS             complement(<1..306)
                     /locus_tag="ACZPDN_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297412.1"
                     /translation="MIPYFEILEFGKVKKRFREALSTISFSNELMTVPEMQITIPNEY
                     YDLISGRKEMRVIMDCGVFYGMITDYKPSVSGLNISLTHVINEWTYRQVPTNYAVKNA
                     "
     gene            complement(315..1031)
                     /locus_tag="ACZPDN_01915"
     CDS             complement(315..1031)
                     /locus_tag="ACZPDN_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297413.1"
                     /translation="MAWQNFHGAYDNTGPYANVVLGGNPGDTADFGFPLAIAHSKGYG
                     KGINFSDDGNYGVTFTLDLVGYGVTDAGQYTGNGKYVQYGGRYNYILIISVSNNNKAS
                     WRQIYNQVIFSHADTWPLAYSSGWEIVAQNSQWSGKLQLPTDTTHVKVELRGEDATFP
                     YENIYSIQQVIPDFRPWAVRKGGIFYSLDRATGWFKKRVKDSWVTIGKYSADKANKEN
                     QGSSRIRKNGKWVGQGKIGS"
     gene            complement(1031..1633)
                     /locus_tag="ACZPDN_01920"
     CDS             complement(1031..1633)
                     /locus_tag="ACZPDN_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297414.1"
                     /translation="MNKDVCSACDSLKATSSNFIQKGVTDTICANLKANQGFENKGHN
                     NCTDMHDMNDCLLGGLLEKIDAMDVCDTKEAIKDLEKNLISIIDVMICSDCGQWEEIE
                     KLWAEIQKLWNAIRDLQGKVGKLEGSVCDMYSAVEKILTNLKNSGAWKQTGDTVFQGK
                     FNDGRSIATGNINIFGGTPDGNSYIRTNNGSSENDLAGGV"
     gene            complement(1647..2492)
                     /locus_tag="ACZPDN_01925"
     CDS             complement(1647..2492)
                     /locus_tag="ACZPDN_01925"
                     /inference="COORDINATES: protein motif:HMM:NF037758.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297415.1"
                     /translation="MANVKSYCTACGKLKDSSAEFIQNGVTDSICTSLGNDTGLNPEN
                     GNNTCTDMENANDCLTKGLYDVLDGFDLCDWKLFMSQYANNDYNMKAAMICWMCGLQD
                     QLYNLQLQNLAIETQYTIQQSTPGLSVAIDRQGNFRFNYSDWIHTSGYTKVADGVITG
                     KVDFCMKPNKDKSATYKFNSVTLKHYSYKMTGVSAGSAPTISIRVPNRSGSLVYQKIT
                     NASFEEDINKTVELSMSGTVKAGETTNWLQFLSIYVDWVEDDEISLHTRFVNDNKVNF
                     VICRD"
     gene            complement(2504..3580)
                     /locus_tag="ACZPDN_01930"
     CDS             complement(2504..3580)
                     /locus_tag="ACZPDN_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297416.1"
                     /translation="MTCYNLNPLKTYVQFNDLVIDSAEEISSASLKQDTKTETQEYSY
                     GHGSYVAFQKNQQFLTEGDLSLTLNFNYEHFHDEDRRFLRDYFNLNLLKPGRLWAIQD
                     NKLIWAWAYVTGFSEDYKKYQGYLSMDIDFKLWEGVWHIADTKKTFLVPYSVCNILDC
                     EDFRDAQECLSCCVTCPPDMETCNSCLCDCGDITEETSLCVMGTKALEDFMNCGNSYK
                     IVYDCIKGEQIFGDDLIKNKICKKDYCVESIAGRFYSGTVLDTDKVKLILDGKFQNPE
                     IEINGNKMMILGEYDGILTLDSSWNLYFTADGCCSSEEVDLDNLVIEDEFGFTVHHGM
                     NRLVVTGSCCKMACVYIDVDELTN"
     gene            complement(3580..8163)
                     /locus_tag="ACZPDN_01935"
     CDS             complement(3580..8163)
                     /locus_tag="ACZPDN_01935"
                     /inference="COORDINATES: protein motif:HMM:NF040129.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HIR1297417.1"
                     /translation="MSVREVGARLVLDIKDAEAKIKQLEKELKDIEKAKLKVDANTNE
                     LEKIKARLEEIKKEKEALERQKLSLKVDLDNLANLKNQLLDVKDDINELKKELLALSN
                     KKLSIDIDLKANANEIHDVINDKTLGENDRSDKLKGLYSAREALKYDMREVGIEIDEV
                     QKKINNLNKEKIKIEANISELNDAQKLVDEIDDSIADLDKEKIKLEADYSKLEDTNKK
                     LDETIEKENDVRSTKADIESQVIGYQDSLSKLNNLQQAAKALKTASKITFDVGNKMSN
                     LGSSMLNIAKNFQNNPIGDIGRFLVQGVGYSSLYRLVSSTQNAIGDALSNGVKRYDTI
                     NVAKRTLSTVVGDVEDSTTKIQKMIDNLDESILGLPTTLDDALSHVTRFTSINHDLDR
                     SQKLFSAINDSILTFGGDSEGVNNAVTQYSQIMGSKMDARTLRSMEDAGMTPALTAIA
                     KKFNMSFAEFREAFTGSNPTISLQQFEDALIELDEKGGGGLNSLATMVKSSVSTITNA
                     FDLIPKRFSKAEEKWLGALDEVSTELTGATIYGNIYKLSQKVEGLGDIGANFIRSHKK
                     EIGEGIDFIKTKFSELWSVLKTFSFKDFVGGFKQGLDDFKGAIDFFKPLVGDLYDFAK
                     DKITEMGDGSFSKGLGRFVSDYIQIGIGLKYAGKLMKLGSGGISLLGDLLNVYSKFKG
                     KSFNIPFLGKLGSKFSSIKNVFKSSDDITAATSTPKTFDVEGFKNKLSSLAIIAGGAG
                     TIILYCKAIKEIEKNVPNDITTLPMRLTNLFSVMGLMMGANTINAGVSKALEMNNALT
                     GLAMMIGQGGALWLFAKAMQELDKTMPDGFDTFNDKLLGLFECIGSMTLITGIQGGAG
                     VLTGGITTLAQVLGMITTTGLAGTLIACAKAMQEVDKNVPSNAKGLKKKIQGIMDVID
                     MFEGGGTLSSWWSQVIKSSESLWKNMETWNITRILKKLVTIGESISKVQGMSIDSSSF
                     NDQFKDIQEVIKNINDFEFPTVSTSSATNIADANSIVKNYTTMASSLSKMSSINGSSI
                     NVENCTSILKNVASVVQEMKKIVFHDVTKSIKSNLNSTNAQEFLDTLKILEQIVPEFG
                     NLQATITNNPLPKAEDIKKTIESISQAIGYISVAGVGTGKDKNMLSYNLRQMPDSKLF
                     NNALKAITTLGDIILKFGTLNVYSTDFDFETLKANIKSIGNAVNEMATNKGLTENLEN
                     MDTVNKTVSKLKKTCESLNSIVGLNLDFVKVGEVTTGIQTFLNNVKGLKVGEATTTIV
                     SEVNSIVTSFHNMATTLSNMKSEFNTSGTDMANGIIEGFKSIDIEGSFGTKIDNAKAS
                     LKKKSFKSVGKKFGKDVVSGFSEGISNMSSSISNQITMMYGYSTRFTDLGQYLGSAFK
                     NAFNNQSGNINTGGTTTPTVNRGNESKGNNMKFAQGGPVYLKRGGQPIVMKPSGTDTV
                     PAMLTPGEYVMKRSAVKKAGQSFMDKVNNMDLKGAFKELSTRYGSQVGSVVNKNVTIN
                     NNDNRVTNNSIAFNEGNERRQAIKVGRCLRGLA"
     gene            complement(8150..8392)
                     /locus_tag="ACZPDN_01940"
     CDS             complement(8150..8392)
                     /locus_tag="ACZPDN_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297418.1"
                     /translation="MAHYVGKILKIRPNEILDHWGVSELVVAFGYYANLQSDKTWNEI
                     NEANKNSKKKIPQIDRYAVHFIQKTDLAKESEDVST"
     gene            complement(8470..9180)
                     /locus_tag="ACZPDN_01945"
     CDS             complement(8470..9180)
                     /locus_tag="ACZPDN_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297419.1"
                     /translation="MEEQKILEPTQLNAMIEKLKVAREDDTPHAVYGNGGEIAVVGDA
                     NKTDVKTIDIEVNFRFTEKEIEEHKIDVPENAKRVGQYVMFDKKFENLTLSPRQDMKM
                     VEALIEVKPLLLDAEQILDPYKEKFQEIEEYYGHKFIEGKDGIVTTDADDEEVNKTMV
                     KIYEAYMKEANEQIFHLYAQSSTNLVDGLYKVVAIFLGLDEFYEDHMMQYSVLTCMIS
                     LIIKYPELFNEVETVFIK"
     gene            complement(9200..10606)
                     /locus_tag="ACZPDN_01950"
     CDS             complement(9200..10606)
                     /locus_tag="ACZPDN_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297420.1"
                     /translation="MSNCNINKIISDKLSVSKLTKTQEIDITIMSDIDSCLKINTRKF
                     EKITGTSSAYTSRTIAPDLINVCESFGCKNTGTLFITSKETDAEGSDGNKIHTSGAVF
                     KALKNALDFAAGVVYYYVNVPQAGTYTITTKISDVLDHEMTNADEYTTTLKADKEGFY
                     PVQIDLSTVPTKVSGTGWEASTSGVRLSIEVALTDKSSDSILIGLSSISFFEEFADLD
                     SNNDIKVSCLSGFDGDDTVDPVDTSCFDDSYDDDSASIERSFTGTQLTSNYLTMNPFI
                     GKGDKSQGFMMRTQEVVIEADKEHPEYGSIHIADHFVEECGFIYAALSDQCNITDSTL
                     NRINTPLLANLDESQYQVLNSKINPSLDIEGSKIYFNKNLVGKTLKISYPMTVDVLQH
                     YVANNDSLKNKRAKVTITRYRSDGTAEVFTYHNAKITSFPMGIPDDGAFEFSLAFKKD
                     TRGNWYEVYVVNKANANL"
     gene            complement(10606..10767)
                     /locus_tag="ACZPDN_01955"
     CDS             complement(10606..10767)
                     /locus_tag="ACZPDN_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297421.1"
                     /translation="MEEKVVKAKKTPEQKVDVQAFVSRKLNALNQLGGAKAERAMERV
                     LKATMGGQK"
     gene            complement(10780..11235)
                     /locus_tag="ACZPDN_01960"
     CDS             complement(10780..11235)
                     /locus_tag="ACZPDN_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297422.1"
                     /translation="MLKGLLEDVIEACEEDFEGLASELEETMREEAPRGSRFYAQEMT
                     SMPWNEYRPGALKDSITKEKVSNTEYLIGVDADKLEKDSRNPSHVDYSPMVQNGTKRV
                     YTLVRKNGRPFVWVDEMGKKHFAHRIKMPPRKANDFVARAVSRFDAKVK"
     gene            complement(11478..12191)
                     /locus_tag="ACZPDN_01965"
     CDS             complement(11478..12191)
                     /locus_tag="ACZPDN_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297423.1"
                     /translation="MYEKIVEQLKNYCSCIKESDLEADKLEKNVGELIDLISTITCWK
                     NHPCETFLSSQREEVFDVGEFKKCGCDSGIVRIPLFYPMIDPTTIEVSVITRERITFT
                     THKLEVDKDFSYNPYENIVYVDLSNIDYKDVCNCGCDELSKIVVSYVAGYETIPECLL
                     PVFCDFLQFVIAMNRCECGCSTCEETDGSDVLISEENSDAQISISMYVREHITKAYSE
                     QLGILSVCNSKDTWVGAVV"
     gene            complement(12191..13453)
                     /locus_tag="ACZPDN_01970"
     CDS             complement(12191..13453)
                     /locus_tag="ACZPDN_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297424.1"
                     /translation="MLDLLFTNPDNTLLEKMAVTPGMVERLSSNIEDLTSFSKAYIDY
                     EKARQNLAASSTKSNAGTVGISTDYSDNSPANPFQNVFPLVSWLMNTPASRKMQGAMN
                     RGAWSVTKKEDGKFYIQLPFTYGTTEPKSTQGECCWVPLDLAKCGSNAPLALLCLKSC
                     EPIMDSLVNETRKIKANDMVCYFQREGETIKEAQKRMDLISMAYFTAINVILGTMATG
                     TATLKPFHGLLEVMEDKAVIKIVGTNVLSAFDSVGLRLAALGDGDYKFACHPLVLEGI
                     KSVIVPGKFNGEYPDGWTRNRETGEIAFKGHGFIADKLVPCDITKGTGDVWVLEGNTV
                     GLVMGTTFQPSEKFQRHTFGATDTPSEGCGTQCDYYYNFGCAFGTDANRLMVIQGIPM
                     SAATLGDTLNGLDLVLKPTTIVPINIGE"
     gene            complement(13464..14534)
                     /locus_tag="ACZPDN_01975"
     CDS             complement(13464..14534)
                     /locus_tag="ACZPDN_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297425.1"
                     /translation="MPQKKRVPTYVASIKDSIDRRKKGKAFYDNAITLSSVDKENHYV
                     SVNLSSGYVENKPTRLIDEGAITYEGGNDIRLYIKKGAVQAFYDSLSSDYVGYINLAH
                     IDIASLPLNLGTWTKDDLTVVDIGDGRKGLDVNVKLNRELHIVQDLLKQEIPLSISAE
                     LRGTLDLESSFKFNAPFYNEIEIAGFSVVANPANVNSTGENLNSKGDSEMNLWEKILK
                     LSSENKEEKKNDALENKEEEKEEKELESKEEGTENEEEAKKGEETLETVEMSKDDMEK
                     INKFMDAFEALSAKVEALETENAELKEKLKNSKKEKTEFEKKAESTLDRLSSLISGQA
                     NDKEKKEEKLSSTSNVSGDMWG"
     gene            complement(14534..14974)
                     /locus_tag="ACZPDN_01980"
     CDS             complement(14534..14974)
                     /locus_tag="ACZPDN_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297426.1"
                     /translation="MQNNVQTINGVTWFDSLEERNAFLKQNGRHEFALEEAAKNAKQY
                     LKLLDVIEEKTQIDVYSKLDSGTLLYGYVVLEPKKKYKIPEDKVLLEALRNKTIQKRY
                     DSTMEEILKGAKIPYEVKKCNSCGGRIQKLFYKPVIVVETETKK"
     gene            complement(14986..15279)
                     /locus_tag="ACZPDN_01985"
     CDS             complement(14986..15279)
                     /locus_tag="ACZPDN_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297427.1"
                     /translation="MSIKRSFTVKITFKEGYGNPITLTGKDATDFNTAWHNKLNDQDG
                     AIGFEWPVITTTGESPNQKTVTTYTSFLFCNVAKVERSEQTETKYTDDQCHDA"
     gene            complement(15421..16008)
                     /locus_tag="ACZPDN_01990"
     CDS             complement(15421..16008)
                     /locus_tag="ACZPDN_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297428.1"
                     /translation="MEMDILKSILSENEVTPLGSLNGTPLYSFEDAQRINKIGLVKEK
                     IQGKEVEFGERPMRPDGLGYLETKSNAIAVPTSFFENRYRKVEVKETIVDEKTKKEKE
                     VVKDVYYEVVTDYRACKEQASGRVYTTTIHVYQIGAKKDSKGNADLFLIGQKNISDTE
                     FINEFKGKLNKESMVKILKLIGNNKTEQVEDTLEF"
     gene            complement(16026..17447)
                     /locus_tag="ACZPDN_01995"
     CDS             complement(16026..17447)
                     /locus_tag="ACZPDN_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297429.1"
                     /translation="MSRRTKKRQKDRVKLASNTYVSPNISQNIHSSNAETEAEKVMEA
                     MLNCNSDCINGFIKTNFKNQFDEIDWMIDNLPTLPYVIGKVIDFIFSNGITTGDENLD
                     KNVLMPFLYRHNVQGVTNYSVLQNAIMQSLLYGKCGIRWLDEDKGIVTENYRNYVSIM
                     REDDEYKGFRVPICYAMSANDKEPISLGTKEIDFDEALFLKTGKLMSKDGTIIVEIPD
                     NFCNLRNGTDNENGLSCLLRDKQRLKLLGAVYERLNYDIQYDGPGRLIFWLKDGFAKG
                     DTIDLSASQVLDESSSSKADRADKARIEAKRLGQEIRNSKSDNVILASSIFEKMDHLP
                     RVTKGTEFLEYLQMKEGSIICQCFGITPELIGLGDVSGNVSMERIIDNAMTNTIVPMR
                     ERFATQISPMLSEKLGVPKVYFDKYELKEQQDKSAKTYKLALSVTQIVGAIVNGAEAL
                     DKSTKNYMMESVTRMMDSIEKTL"
     gene            complement(17459..19102)
                     /locus_tag="ACZPDN_02000"
     CDS             complement(17459..19102)
                     /locus_tag="ACZPDN_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297430.1"
                     /translation="MKKQTLSVKEQYFNDVEDLKEAKAINKSQEEVVRLLKEATPKYK
                     LKKWTRGYIPEHYKRLNISRQEAFRLAVIGAREALTYFQVNLHFTQAMLFGAVVEGYD
                     TIYVITTSQYGKSWTLGMIAIYRAYKGHQVRIAAATGETATIIMSKVIGHLQNADESI
                     QSSVLDSGNKIEKLQTSTSKTKISFKGGGCVEIVTLGGNSVDPKKNNNAIGKGGDYII
                     DEAAQVSEDAYAEIGRREFSSVDGSKELEIAISNPHKRGEFYDCMTNDKYPEGTLVVW
                     MDVRTAYEEDRMKSASQILNSHFYKNRSTCQRYLVCELEEFSDESMFKTMTLDDDKDD
                     SSYKKRFFLGVDSAYTGKDGIDVALCSQNRYGYCKIETIYNLKEGVWVQGVTSEKIIT
                     KIVKIIETLNIKYVCVDVGFGTWLTEGLSKYSDKLGFILEGVNFQGGPTKTRIKARHY
                     SAVYAFNLRAEMYLDFQQLMDSKKLTFTTEVAKRLKPELLATRTVSKNNKKIAIIPKE
                     EIKQRLGHSPDALDSSVLSVRSCLMYNLSGEILAYAEND"
     gene            complement(19083..19592)
                     /locus_tag="ACZPDN_02005"
     CDS             complement(19083..19592)
                     /locus_tag="ACZPDN_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HIR1297431.1"
                     /translation="MPRAKSVSELKREDEAKRFFDEYSKSGNITKSMQKIRPDLSDKS
                     AYNKGYKILNSPLFRNVIHERVKKRDQRSVMTVEQRRQWLSDNIQDEEKDMKDRLGCL
                     KELNRMDGIGKSNILNVGSVNNITVEQKRAIAEERINDILGIKMGSEFLDAEVIEHEE
                     DENSEETDS"
BASE COUNT         5715 a         4136 c         2941 g         7431 t
ORIGIN      
        1 agcgttttta accgcataat tcgttgggac ttgtctatat gtccattcgt taattacgtg
       61 cgttagagat atgtttaaac cacttacaga tggtttgtag tcggtaatca ttccgtagaa
      121 aactccacaa tccataatta ctcgcatttc ttttcttcct gatattaaat cgtagtattc
      181 gttaggaatt gtgatttgca tttcaggtac tgtcatcaac tcatttgaaa aactgattgt
      241 gcttaaagct tctctgaatc ttttcttaac ttttccaaat tctaatattt caaagtaagg
      301 aatcatattt actcctaact accaattttg ccttgtccta cccatttacc attttttctg
      361 attctacttg atccttggtt ttctttattt gctttatcgg cactgtactt gccaatagtt
      421 acccaagagt ctttaactct ctttttaaac catcctgtag ctctatccaa agaatagaat
      481 ataccgcctt ttctgactgc ccatggtctg aaatcaggga taacctgttg aattgaatat
      541 atattctcgt aagggaatgt agcatcttca cctcttaatt caactttaac gtgtgttgta
      601 tccgttggaa gttgtagctt accactccat tgactgtttt gtgccactat ttcccaacct
      661 gatgaataag ctaatggcca tgtatctgca tgagagaata ttacttgatt gtaaatctgt
      721 ctccatgagg ctttgttgtt gttagaaaca ctaatgatca aaatatagtt gtatcttccg
      781 ccatactgta catactttcc atttcctgta tattgaccag catccgttac accatatcca
      841 actaaatcta atgtgaatgt aacaccatag tttccatcat ctgaaaagtt gatacctttt
      901 ccatatcctt tagaatgggc aatagcaagc gggaatccaa agtctgcggt atcacctgga
      961 tttccaccta atactacgtt tgcgtatggc cctgtgttat cgtaagctcc atgaaagttt
     1021 tgccatgcca ttaaacacca ccagccaaat cattctcaga acttccgtta ttagtacgga
     1081 tatatgagtt tccatcagga gtaccaccaa agatattgat attacctgtt gcaatgcttc
     1141 ttccgtcatt gaattttcct tgaaatacag tatctcccgt ttgtttccat gcaccactgt
     1201 ttttaagatt agtaagaatc ttttcaaccg cactgtacat atcacaaaca ctgccttcaa
     1261 gctttccaac tttgccttgt aaatccctga tagcattcca aagtttttgg atttctgccc
     1321 atagtttctc gatttcttcc cattgtccgc aatcagaaca aatcattaca tctatgatac
     1381 tgattaaatt cttttccaaa tctttgatag cttcttttgt atcgcaaaca tccattgcat
     1441 caatcttttc taacaatccg cctaataggc aatcgttcat atcatgcata tctgtacagt
     1501 tattgtggcc cttattttca aagccttggt ttgctttaag atttgcacaa atagtatctg
     1561 ttacaccttt ttgaatgaaa ttactgcttg tagcttttaa agaatcgcaa gcagaacaaa
     1621 catctttatt catttatgtg tacctcctaa tctctacaga taacaaaatt caccttgtta
     1681 tcatttacaa aacgagtgtg tagagatatt tcatcatctt ctacccaatc aacataaata
     1741 gaaaggaatt gcaaccaatt tgttgtttct ccagctttta ctgttccact catgcttaat
     1801 tcaactgttt tgttaatatc ttcttcaaat gaagcatttg tgattttctg atataccaac
     1861 gacccactcc tattaggaac acgaatcgaa atagtaggag ctgaacctgc tgaaactcca
     1921 gtcattttat aagagtagtg tttcaatgta acgctattga atttgtatgt agcactctta
     1981 tctttgtttg gtttcataca gaaatcaact tttcccgtaa taactccgtc cgctactttc
     2041 gtatatccac ttgtatgaat ccaatctgaa taattgaatc tgaaattacc ttgtctgtca
     2101 attgcaacac tcaatccagg tgtagactgt tgaatagtgt attgagtttc gattgccaaa
     2161 ttttgaagtt gaagattata caactggtct tgcaatccac acatccaaca aatcatagct
     2221 gctttcatgt tgtaatcatt gttagcatat tgactcatga ataatttcca atcgcacaaa
     2281 tcaaatccgt ctaggacatc atacaagcct tttgtaaggc aatcgttggc gttttccatg
     2341 tctgtacacg tattattgcc attttcggga tttaagcctg tatcgtttcc taaagacgta
     2401 cagattgaat ctgtaacacc attttggata aactctgcac tgctatcttt taactttcca
     2461 caagcagtgc aataactttt tacatttgcc actgcaagcc tccttaattt gtaagttcat
     2521 caacatctat atatacacaa gccatcttac aacatgagcc tgtgacaact aatctattca
     2581 ttccatgatg tactgtgaat ccaaattcat cttcgatcac tagattatct aaatctactt
     2641 cctctgatga acaacatcca tccgcagtaa agtataagtt ccaacttgaa tcaagtgtta
     2701 aaattccatc atattcacct agaatcatca tcttgtttcc gttgatttca atttcagggt
     2761 tttggaattt accatctaga atcaatttta ctttgtcggt atctaatact gttccactgt
     2821 agaatcttcc agcaattgac tcaacacaat aatctttttt acatattttg ttcttaatca
     2881 aatcatcacc gaaaatctgt tcacctttga tgcaatcgta gacaatcttg tatgaattgc
     2941 cacaattcat aaaatcttcc aatgctttag ttcccattac acataaggat gtttcctctg
     3001 ttatgtctcc acaatcgcat aaacacgaat tgcaagtttc catatcagga gggcaagtaa
     3061 cacaacacga taaacactct tgagcatctc tgaaatcctc acaatcgagg atattacata
     3121 cagagtaagg aactaagaat gttttctttg tgtctgcaat atgccataca ccttcccaaa
     3181 gtttaaaatc aatatccatt gatagataac cttggtattt tttgtaatct tcactaaatc
     3241 ctgtgacata ggcccatgcc caaatcaatt tgttatcttg aatcgcccat aaccttccag
     3301 gtttaagcaa attcaaattg aaatagtcac gtaggaatct tctatcttca tcatgaaaat
     3361 gttcataatt aaaattcaat gttaaggaca agtcaccttc cgtaagaaac tgttgattct
     3421 tttggaaagc aacataacta ccatgcccgt aactatattc ttgcgtttca gtctttgtat
     3481 cttgctttag agaggcagag gaaatctcct ctgcactgtc tattacaaga tcattgaact
     3541 gaacgtatgt ttttaatggg tttaagttat aacaagtcat tatgccaaac ctctcaagca
     3601 tctacctact ttgatagcct gccttctttc gtttccttcg ttgaaagcaa tactgttatt
     3661 cgtaacacga ttatcattat tgttgatagt cacattctta ttaacaacac ttccaacttg
     3721 agaaccatat ctagtagaca attctttgaa cgcacctttc aaatccatgt tatttacttt
     3781 atccatgaag ctttgacctg ctttcttaac tgcactacgt ttcattacat actcaccagg
     3841 agttagcata gcaggcactg tatctgttcc gctaggtttc ataacaatag gttgtcctcc
     3901 tcgttttaag taaactgggc caccttgagc aaacttcata ttgtttcctt tagattcatt
     3961 tcctctgttt actgtaggag ttgttgttcc acctgtatta atgtttcctg actgattgtt
     4021 gaacgcattt ttaaacgcac ttcctaagta ttgtcctaaa tctgtgaatc gtgttgaata
     4081 tccatacatc atagtaatct gattagagat tgaactagac atatttgaga taccttcact
     4141 gaatccactc acaacatctt ttccaaactt cttacctact gatttgaagc ttttcttctt
     4201 caaggaagct ttagcattat caatcttagt tccaaatgag ccttcaatat caatgctttt
     4261 gaaaccttca ataattccat ttgccatgtc tgtaccagag gtattaaatt cagatttcat
     4321 atttgataaa gttgtggcca tattgtggaa tgaagtaacg attgagttta cctctgaaac
     4381 aatagttgta gtagcttctc caacttttaa tcccttaaca ttgtttagga atgtttgaat
     4441 gcctgttgtg acttctccaa ccttaacaaa gtctagattt aatccaacga tagaatttaa
     4501 gctttcgcac gtttttttca acttagaaac agtcttatta actgtgtcca tattctctag
     4561 gttttcagtt aatcctttgt ttgttgccat ttcattaact gcatttccaa tactcttaat
     4621 attggctttc agtgtttcaa agtcgaaatc agttgaatat acgtttaaag taccaaattt
     4681 aaggattata tcacctaaag ttgtaatcgc ctttaatgcg ttattaaata gcttagaatc
     4741 aggcatttgt ctcaagttgt aagacaacat attcttgtct tttcctgttc caacaccagc
     4801 tacagaaata tatccaatcg cttgagaaat actctcaatt gtctttttaa tatcctctgc
     4861 ctttggtaaa ggattgtttg taatcgttgc ttgcaagttt ccaaattcag gaacaatctg
     4921 ttccaaaatc ttcagtgtat ctaggaactc ttgagcattc gtagagttta aattagattt
     4981 aatactcttt gtaacatcat ggaatacaat cttcttcatt tcttgaacaa cactagctac
     5041 attctttaaa atgcttgtac aattctcaac gttaattgaa cttccattta tactagacat
     5101 tttagaaagg ctagaagcca ttgttgtata gttcttaacg atactgtttg catccgcaat
     5161 gtttgttgca cttgatgtac tgactgttgg gaactcaaaa tcattaatat tcttgattac
     5221 ctcttgaata tctttgaatt gatcgttgaa agaactacta tcaatactca ttccttgcac
     5281 ttttgaaatt gattctccaa tagtaacaag tttctttaga atcctagtga tgttccaagt
     5341 ctccatattc ttccataaag actcagaact tttaataact tgactccacc aagaagacaa
     5401 tgttcctccg ccttcaaaca tatcaatgac atccataatt ccttggattt tctttttaag
     5461 tccttttgcg tttgaaggaa cattcttatc gacttcttgc atagctttag cacaagcaat
     5521 caatgtacca gctagtcctg ttgttgttat cattcctagc acttgggcca atgtagtgat
     5581 tccacccgtt aggacaccag caccgccttg aatgcctgta ataagtgtca tagagccaat
     5641 gcactcaaat aagcctaata acttatcgtt gaatgtgtcg aatccatcag gcatggtttt
     5701 atctagctct tgcatagctt ttgcgaatag ccataaagct ccaccttgac caatcatcat
     5761 tgccaatcct gttaaggcat tgttcatctc taatgctttt gaaactcctg cattaatcgt
     5821 gttagctccc atcatcaatc ccattacaga gaatagattt gttaatcgca ttggcaatgt
     5881 agtaatgtca tttggaacat tcttttcaat ttcctttata gctttgcaat aaagtataat
     5941 tgttcctgcc ccaccagcta tgatagctaa tgaagataat ttatttttaa atccttctac
     6001 atcaaaagtt tttggagtgc ttgtagcagc agtaatatca tctgaacttt tgaatacatt
     6061 tttaatagaa ctaaatttac ttcctagttt tcctaggaaa ggaatattga aactctttcc
     6121 tttgaatttt gaataaacgt ttaataaatc tcctaaaaga ctaattccac cgcttcctag
     6181 tttcattaac ttaccagcat actttaatcc aataccaatt tggatgtagt ctgatacgaa
     6241 acgtcctaat cctttagaaa agcttccgtc tcccatttca gtgattttat cttttgcgaa
     6301 atcatataga tcaccaacaa gtggtttgaa gaaatctatt gctcctttga aatcatctaa
     6361 tccttgttta aatccaccaa caaaatcttt gaaactgaat gtttttaaaa cactccataa
     6421 ttcagagaac ttcgttttaa tgaagtctat accttcgcca atctcttttt tatgacttct
     6481 aatgaagttt gctcctatat ctcctaagcc ttcaactttt tgagaaagtt tgtagatatt
     6541 tccgtagatt gtagctcctg tcaattctgt tgaaacctca tctaatgcac ctaaccactt
     6601 ttcttcggct ttactaaatc tctttgggat taagtcgaaa gcgtttgtga ttgtagatac
     6661 agacgattta accatagttg ccaacgaatt taggccacca ccgccttttt catccaactc
     6721 aattaaggca tcctcaaact gttgtaatga aatagttgga tttgaacctg taaatgcttc
     6781 tctaaactct gcaaatgaca tattaaattt ctttgcaata gcagttaagg ctggtgtcat
     6841 acctgcatct tccattgatc tcaatgtacg agcatccatt ttagaaccca tgatttgaga
     6901 atactgagta accgcattgt ttaccccctc agaatcacca ccgaatgtca aaatggaatc
     6961 attaattgcc gagaatagct tttgagacct atctaaatca tgattgattg aagtaaatct
     7021 cgtaacatgg cttagagcat catctaaagt ggttggtagg cccaaaatgc tttcatctag
     7081 gttatcaatc atcttttgga ttttcgttgt agaatcttct acatcaccta ctacagtgga
     7141 taatgttctt ttcgcaacgt tgattgtgtc gtatctttta acaccatttg ataatgcatc
     7201 accaattgcg ttttgtgtac ttgaaaccaa tctatacaaa ctagaatatc caacaccttg
     7261 tactaagaat cgtccaatat ctcctattgg attgttttgg aaattcttgg caatgttcaa
     7321 catactagag cctagatttg acatcttgtt tccaacatca aatgtaatct tactagcagt
     7381 tttcaaagct ttagcagctt gttgaagatt gtttagttta ctcaagctat cttgataacc
     7441 gataacttgt gactcaatat ctgcttttgt gcttcttaca tcattctctt tttcaatggt
     7501 ttcatctagc tttttatttg tatcttctaa cttagaataa tcagcttcta attttatttt
     7561 ttctttgtct aaatctgcga ttgaatcatc aatctcatca accaattttt gagcatcatt
     7621 taattcactg atgttcgctt caatcttaat tttttctttg ttaagattgt taattttctt
     7681 ttgaacttca tcaatttcaa tgccaacctc tcgcatatcg tatttgagag cttcacgtgc
     7741 actatataat cctttaagct tgtcgcttct atcgttttca cctagtgtct tgtcattaat
     7801 gacatcatga atttcattgg catttgcttt taaatcaata tcaatagaaa gttttttatt
     7861 gctcaaggct aatagctctt ttttaagttc attaatatca tctttaacat ccaataattg
     7921 attctttaaa ttagctagat tatccaaatc aacttttaaa gaaagttttt gcctttccaa
     7981 agcttccttt tcttttttga tttcttctaa tcttgcctta attttttcta attcattggt
     8041 gttagcatca actttgagct ttgccttttc aatatctttt aactcttttt caagttgttt
     8101 tatctttgct tctgcatcct taatgtcaag gactaaccta gcaccgactt cacgtactga
     8161 catcttcgga ctccttcgct aaatctgttt tctgaatgaa atgaaccgca tatctgtcaa
     8221 tctgaggtat tttcttttta gaatttttat ttgcctcgtt gatttcattc catgttttat
     8281 cgctttgtag atttgcgtag tacccaaagg ctacaactaa ttcagaaaca ccccaatggt
     8341 ctaatatctc attggggcgt atttttagaa ttttaccgac ataatgagcc atggttgaat
     8401 aaagatttag ttctgcaaca taagactttg ctttttttac tgaatccttt ttatcatccc
     8461 ccttatcaat tatttgataa aaactgtttc tacctcattg aataattcag gatatttgat
     8521 aattaggcta atcatgcaag ttaaaactga atattgcatc atgtgatctt cataaaattc
     8581 atctaatcct aagaaaattg caacaacttt ataaagtcca tcaactaaat ttgtagagga
     8641 ttgagcgtat aaatggaaaa tctgttcatt tgcttctttc atatacgctt cataaatctt
     8701 aaccatagtc ttgttcactt cttcatcatc tgcatctgtt gtaacaattc cgtcttttcc
     8761 ttcaatgaat ttgtgaccgt aatattcctc gatttcttgg aatttttctt tatatgggtc
     8821 taggatttgt tctgcatcca atagcaatgg ttttacttcg attaaagctt ctaccatctt
     8881 catatcttgt ctaggagata atgttagatt ttcaaacttc ttatcgaaca taacgtattg
     8941 ccctactctt ttagcattct caggaacatc aattttatgt tcttcgattt ctttttcagt
     9001 gaatctaaaa ttcacttcaa tatcaattgt tttaacatct gtcttatttg catcaccaac
     9061 aactgcaatt tcaccaccat tgccatagac tgcgtgagga gtatcatcct cacgagctac
     9121 ttttaacttt tcaatcatgg catttaactg tgttggttct aaaatctttt gttcttccat
     9181 ctcatttgcc tctcaatttc tataaattag cgttagcttt gtttactaca tatacttcat
     9241 accagtttcc acgagtatct ttcttgaacg ctaaactaaa ttcaaacgct ccgtcatcag
     9301 ggatacccat tgggaatgaa gtaatttttg cattgtggta agtaaatact tccgcagttc
     9361 catcacttct ataacgagtg attgtaactt tcgctctctt attctttaag ctatcgttgt
     9421 ttgctacata gtgttgcaat acatcaacag tcattggata agaaatcttt aatgttttac
     9481 ctactaagtt tttgttgaag taaatttttg aaccttcaat atctaagctt ggattgattt
     9541 tactgttcaa tacctggtat tgagactcat ctaagttagc caacaatgga gtgttgattc
     9601 gattcaaagt agaatctgtg atattgcatt ggtcactcaa tgctgcatag ataaatccac
     9661 attcttcaac aaagtggtct gcaatatgaa ttgaaccata ttcaggatgt tctttatctg
     9721 cttcaataac tacttcctga gtacgcatca taaagccttg agacttatct cccttgccaa
     9781 tgaatgggtt catagttaag tagttagatg ttaattgagt acctgtaaat gaacgctcaa
     9841 tagaagcaga atcatcatca taagaatcat caaagcaact tgtatctaca gggtctacag
     9901 tgtcgtcacc atcaaatcct gataagcagc ttactttaat gtcattgtta gaatctaagt
     9961 ctgcaaattc ttcaaagaaa gaaattgaag aaagaccaat caagatacta tctgatgatt
    10021 tatctgttaa tgctacttca atacttaaac ggacaccaga tgtacttgct tcccatcctg
    10081 ttcctgatac ttttgtagga actgttgata aatcaatctg tacagggtag aatccttctt
    10141 tatctgcttt taaagtggtt gtatattcat ctgcattagt catttcatga tctaaaacat
    10201 ctgaaatctt tgttgtgatt gtgtaagtac ctgcttgagg aacatttacg tagtagtaaa
    10261 caacacctgc tgcaaagtct aatgcatttt tcaatgcttt aaatactgca ccgcttgtgt
    10321 gtattttgtt tccgtctgaa ccttctgcat ctgtttcttt agaagtgatg aacaatgtac
    10381 ctgtattctt acatccaaat gattcgcaaa cgttgattaa atcaggtgca atagtacgtg
    10441 atgtataagc actagaagta cctgtaatct tttcaaattt acgagtgttg atttttaaac
    10501 aagaatcaat atcactcatg atagtaatat caatttcttg agttttagtt aatttagaga
    10561 cacttaattt gtcactaatg attttgttaa tgttgcagtt agacattatt tttgccctcc
    10621 cattgtagct tttagtacac gctccatagc acgctctgct ttagcaccgc ctaattgatt
    10681 taaagcgttt aatttgcgtg aaacaaatgc ttgaacatct actttctgtt caggagtctt
    10741 tttagctttt acaacttttt cttccatttt taatctcctt tatttaactt ttgcatcaaa
    10801 tctagatacc gctctagcaa caaaatcatt tgcctttcta ggtggcatct taattctgtg
    10861 tgcaaagtgt ttctttccca tttcatctac ccaaacgaat ggtcttccgt ttttacgaac
    10921 taacgtataa actcgtttag ttccattctg taccattggt gagtaatcaa cgtgagaagg
    10981 gtttctagaa tctttttcta gtttgtctgc atctactccg attagatatt cggtattaga
    11041 tactttttct ttcgtgatcg aatcctttaa agcacctggc ctatattcat tccatggcat
    11101 acttgtcatt tcttgagcat agaatctact ccctcttgga gcttcttctc gcatagtttc
    11161 ctctaattca ctagccaatc cttcaaaatc ttcttcacac gcttctataa catcttctaa
    11221 gaggcctttt agcatttact acacctcgat aaatgggtaa taaagtttgc ctccatagac
    11281 gtatttaaat ccttttagga atacaccatc ttcatacgat acttcctcaa ctttgttcat
    11341 aaggaagatt tttactaggc cactaggcaa acacatacgt tttgaatact cgtaagatgt
    11401 gttagatttt gctttcgcac cgcatacagg gcatccgttt ttctttgtgg aacttttcat
    11461 tccaatatat ttaattctca tactactgca ccaacccatg tgtcttttga attacatact
    11521 gacaagatac ctaattgctc tgaatacgct tttgtaatat gctcacgaac atacatacta
    11581 attgaaatct gagcatcaga attttcttct gagataagaa catcactacc atctgtttct
    11641 tcacaagtgc tacaaccaca ttcgcatcta ttcattgcga taacgaattg taggaagtcg
    11701 cagaatacag gcagtagaca ttcaggtatc gtttcatatc cagctacata actgacaacg
    11761 atcttagata attcatcaca tccacaatta caaacatctt tgtagtcgat attagataaa
    11821 tcaacgtaca ctatattttc gtatgggtta taagaaaaat ctttatcaac ttctagtttg
    11881 tgagtagtaa atgtaattct ttctctagtg ataacagata cttcaatcgt tgttgggtca
    11941 atcattggat agaataacgg tatgcgtaca attcctgaat cgcaaccgca tttcttaaat
    12001 tcaccaacat caaagacttc ctctctttga gatgagagga aagtctcaca aggatggttt
    12061 ttccaacaag tgatggtact aattaaatca attagttctc caacattctt ttcaagctta
    12121 tctgcttcta aatcgctttc ctttatgcac gaacaatagt ttttcaattg ttcgacaatt
    12181 ttttcgtaca ttattcacca atgttgattg gtacgatagt tgttggtttt aatacaaggt
    12241 ctaatccgtt taatgtatct cctaatgtag ctgctgacat tggaatacct tggattacca
    12301 ttaatcggtt tgcatctgtt ccaaatgcac atccaaagtt gtagtagtaa tcacattgag
    12361 taccgcatcc ttcagatggt gtatctgtag caccgaatgt atgacgttgg aatttttcag
    12421 atggttggaa agtagttccc attaccaaac ctactgtatt tccttctaat acccatacat
    12481 cacctgtacc ttttgtgatg tcacatggaa ctaatttatc tgcgataaat ccatgtcctt
    12541 taaatgcgat ttctcctgtt tctctattac gagtccatcc atcaggatat tctccgttga
    12601 atttacctgg aacaataaca gatttaatac cttcgagtac taatgggtga caagcaaatt
    12661 tgtaatcgcc atctcctaat gctgctaaac gtaaaccaac tgaatcaaat gcagataata
    12721 cgtttgtacc tacaattttg ataactgctt tatcttccat tacttccaat aatccatgga
    12781 aaggtttcaa tgtagcagta cctgttgcca ttgttcctaa gattacgtta atagcagtaa
    12841 aatatgccat tgaaattaaa tccatacgtt tctgagcttc tttaatagtt tctccttcac
    12901 gttggaagta gcaaaccata tcattagctt tgattttacg tgtttcattt actaagctat
    12961 ccataatagg ttcgcagctc tttaaacaca ataatgctaa tggtgcattg ctaccgcatt
    13021 tagctaaatc taatggaacc cagcaacatt caccttgtgt tgatttaggt tctgttgttc
    13081 cgtatgtgaa tggcaactga atatagaatt tgccatcttc tttttttgta acactccatg
    13141 ctcctcggtt catagcacct tgcatcttac gtgaagctgg tgtgttcatt aaccaagaaa
    13201 ctaatgggaa cacgttttgg aatggattgg ctggtgagtt atctgaataa tcagtactga
    13261 taccaactgt tcctgcattt gatttagtag aacttgctgc taaattctgt cttgcttttt
    13321 cataatcaat ataagctttt gaaaatgatg ttaaatcctc gatattagaa cttagacgtt
    13381 ctaccattcc tggtgtgact gccatttttt ctaataatgt gttatcagga tttgtaaata
    13441 ataaatctaa catggtttac ctcctatccc cacatatctc cgctaacgtt agaagttgaa
    13501 gataattttt cttctttctt ttctttatcg ttagcttgtc ctgagatcaa actagacaat
    13561 ctgtctaatg tgctttctgc tttcttttca aattctgttt tttctttctt agaatttttt
    13621 aatttttctt ttagttcggc attttctgtt tctaatgctt caacttttgc acttaaagct
    13681 tcaaaagcat ccatgaattt gttgattttt tccatgtcat ccttagacat ttcaacagtt
    13741 tccaatgttt cttcgccttt tttagcttct tcttcgtttt ctgttccttc ttctttactt
    13801 tcaagttctt tttcttcttt ttcttcctct ttgttttcta aagcatcatt cttcttttct
    13861 tctttatttt cagaactcaa ctttaaaatc ttttcccata ggttcatttc tgagtctcct
    13921 ttactgttta aattttcgcc tgtactgttt acattggctg gatttgcaac aactgagaaa
    13981 ccagcaatct cgatttcgtt gtagaatggt gcattaaatt tgaatgacga ttccaaatca
    14041 agtgttcctc tcagttctgc actaatactc aatggtattt cttgtttcaa taaatcttgc
    14101 acaatgtgca attccctgtt tagtttgacg tttacatcaa gaccttttct tccatcacca
    14161 atatcgacaa ctgttaaatc atctttagtc catgtaccaa ggttcaaagg gagtgatgca
    14221 atgtcaatgt gagctaagtt gatatatcct acataatcag aactcaagct atcgtagaac
    14281 gcttgtactg cccctttttt aatgtataga cgaatatcat taccaccctc atatgttatt
    14341 gccccctcgt caataagacg tgtaggtttg ttttctacgt accctgagga taggttcaca
    14401 ctgacataat ggttttcttt atctacgctc gataaagtga ttgcattgtc gtaaaatgct
    14461 tttccttttt ttctacgatc aatgctatct ttaatgcttg ctacatatgt tggaactctt
    14521 ttcttttgtg gcattatttc ttagtctccg tttctactac gattacgggc ttatagaata
    14581 atttctgaat ccttccacca catgaattac atttcttgac ttcgtatgga atctttgctc
    14641 cttttaagat ttcttccatt gttgaatcgt atctcttttg aatagttttg tttctaagtg
    14701 cttctaacaa aactttatct tcgggaatct tgtatttctt cttaggttca agaactacat
    14761 atccgtatag caaagtaccg ctatctaatt ttgaataaac gtcaatttgc gttttttctt
    14821 cgataacatc aagaagtttc aaatactgtt ttgcgttctt tgctgcttct tccaatgcga
    14881 actcatgtct accattttgc tttaagaaag catttctttc ttctagggaa tcgaaccaag
    14941 taacaccgtt aatagtttgt acgttgtttt gcatggtctc tccttctaag catcatggca
    15001 ttgatcgtct gtatactttg tttctgtttg ttctgagcgt tctacttttg ctacattgca
    15061 gaataagaat gaagtataag ttgttactgt tttttgatta ggtgattcac ctgttgtagt
    15121 aataactggc cattcaaatc caatagctcc gtcttggtca ttcaatttgt tatgccaagc
    15181 agtgttaaaa tcagttgcat ctttccctgt taaagtgatt gggtttccgt acccttcttt
    15241 aaaagtgatt tttacagtga aactacgttt aattgacatt tatgtatctc ctttcgttat
    15301 cttgcatata aaaaggcaat acctcgaaat atgcaaaaat ctatatagac agtgaaaact
    15361 gtttatacct tttgtttatt tccaaatatt gccttgtttt tttctacttt ttacttctaa
    15421 ttaaaactct aatgtatctt ctacttgttc tgttttgtta ttaccaatca atttaagaat
    15481 cttgaccatt gattctttgt tcaatttgcc tttgaactcg ttgataaatt ctgtatctga
    15541 aatatttttt tggccaatta agaataaatc agcatttcct tttgaatctt tcttagctcc
    15601 aatctgatat acatgaattg tagttgtata tacacgtcca cttgcttgtt ctttacaagc
    15661 tctgtagtct gttacgactt cgtaatatac atctttaaca acttcttttt ctttcttcgt
    15721 tttttcatca acaatcgtct cttttacttc tacttttctg tatctgttct caaagaaaga
    15781 agttggaact gcaattgcat tggattttgt ttccaaatat cctaatccat caggtcgcat
    15841 aggtctttca ccaaattcaa cctctttacc ttggattttc tcttttacca atccaatttt
    15901 gttgattctc tgtgcatctt caaatgaata taacggagtc ccattcaaac ttcctagggg
    15961 tgttacctca ttttcagata agatactttt taaaatatcc atttccattt tattttctcc
    16021 tctcgctata gcgttttctc gatagaatcc atcattctag taactgattc catcatgtaa
    16081 ttcttagtac tcttgtctaa cgcttctgct ccgttgacaa tagcacctac gatttgagta
    16141 actgacaagg ctaatttata tgtctttgca gacttgtctt gttgttcctt caactcgtat
    16201 ttatcaaaat aaacctttgg cacacctaat ttctcgctta acataggaga aatctgagta
    16261 gcgaaccttt ctcgcattgg cacgattgta tttgtcatgg cattatctat gattctttcc
    16321 atagatacgt ttcctgatac atcccctaaa ccaatcaatt caggagtaat gccgaaacac
    16381 tgacaaataa tagaaccttc cttcatttga aggtattcta agaactccgt accttttgta
    16441 acacgaggca agtgatccat cttttcaaaa atggagcttg caaggattac attgtctgat
    16501 tttgaatttc tgatttcctg acctagacgt ttagcttcaa ttcttgcttt gtcggctctg
    16561 tctgctttag aacttgatga ttcgtctaaa acttgggaag ccgataaatc aatcgtatct
    16621 ccctttgcaa atccgtcttt tagccaaaaa atcaaacgtc cagggccatc atactgaata
    16681 tcgtagttca aacgctcgta aaccgcacct aatagcttta gacgttgttt atcacgcaat
    16741 aaacaagata atccgttctc attgtctgtt ccgtttctta aattgcagaa attatcagga
    16801 atctctacaa tgattgttcc gtcttttgac attaatttgc ctgttttaag gaataacgct
    16861 tcgtcaaagt caatttcctt tgttcctaat gagataggtt ctttgtcgtt tgcagacata
    16921 gcataacaga taggaactct aaagccttta tattcatcat cttcacgcat gatagaaaca
    16981 taattacgat aattctctgt aacaattccc ttatcttcgt ctagccaacg aataccgcat
    17041 tttccgtaca ataatgactg cataatagca ttttgaagta cggaatagtt tgttacaccc
    17101 tgtacattgt gtctataaag gaatggcata agaacattct tgtctaaatt ctcatcaccc
    17161 gttgtgattc cgtttgagaa tataaagtca ataaccttac cgataacata tggcagcgtt
    17221 ggtagattgt ctatcatcca atcaatctcg tcaaactgat tcttaaagtt tgtctttata
    17281 aatccgttga tgcagtctga attgcagttt agcatagctt ccattacctt ttcggcttcg
    17341 gtttctgcat tagaactgtg aatattttgc gaaatgttag gtgacacata ggtattggat
    17401 gctagtttaa ctctatcctt ttgtcttttc tttgttcttc gactcaaatt agcacctcct
    17461 aatcgttctc tgcatacgca agtatttcac cgcttagatt atacattaaa caactgcgga
    17521 cagaaagtac cgaggaatct agggcatcag gagagtgtcc taagcgttgt tttatctcct
    17581 ctttaggaat aatagctatc ttcttattgt tcttcgatac agtccttgta gcaagcaatt
    17641 caggtttcaa tcttttggca acttccgttg tgaaagtcaa tttcttactg tccattagct
    17701 gctgaaagtc taaatacatt tccgctctta gattaaatgc ataaaccgca ctgtaatgtc
    17761 ttgccttgat acgtgttttt gttggcccac cttggaaatt gacaccttca aggataaatc
    17821 ctagcttatc cgagtatttt gacaatcctt cggtcaacca agtaccgaaa ccaacgtcaa
    17881 cacaaacata tttgatgttt aatgtctcga taatcttaac aatcttggta ataatcttct
    17941 cagatgtgac tccttgtacc caaacaccct cttttagatt gtaaattgtc tcgattttac
    18001 agtaaccgta tctgttttga gagcataaag caacatctat accatctttt cctgtataag
    18061 ccgagtcaac acctaagaaa aaacgctttt tataggaact atcgtcttta tcgtcgtcta
    18121 aagtcatggt tttaaacata ctttcatctg aaaattcctc taattcgcat actaaataac
    18181 gttggcaagt acttctattc ttgtaaaaat gagaatttag tatctgagat gcacttttca
    18241 tacgatcttc ttcgtatgca gtacgtacat ccatccaaac aactaatgtt ccttcggggt
    18301 atttgtcgtt tgtcatgcaa tcgtaaaatt cacctcgttt gtgggggttg gaaatagcaa
    18361 tttcaagctc ttttgaaccg tcaacgcttg aaaattccct tcgtcctatc tcggcatacg
    18421 catcttcact gacttgggcc gcttcgtcaa taatataatc tccaccctta ccgatagcgt
    18481 tgttgttttt cttcgggtct acactatttc cacctaatgt aacgatttct acacatcctc
    18541 ctcctttaaa tgaaatctta gttttggaag tagaagtctg taatttttca atcttgtttc
    18601 ctgagtctaa tacagaactc tgaatagact cgtctgcatt ttgtagatgt cctataactt
    18661 tggacatgat gatagtcgca gtttctcctg ttgcggccgc aattcgtact tgatgtcctt
    18721 tatacgcacg ataaatagca atcataccta aagtccagct tttcccgtac tgagaagtag
    18781 taattacata gattgtatcg tacccttcta caaccgcacc gaacaacata gcttgcgtaa
    18841 agtgaagatt gacttgaaaa tatgtcaaag cctctcttgc accgataacc gcaagtctaa
    18901 aagcttcttg tctagaaata ttgagtcgtt tgtaatgttc ggggatatat cctctcgtcc
    18961 atttcttcaa tttatacttc ggggtagctt ccttcaacaa cctaacaact tcttcttggc
    19021 tcttattaat agctttagct tcctttaagt cctctacatc attaaaatac tgttccttaa
    19081 cactaagagt ctgtttcttc actattttca tcctcctcgt gttctattac ttcggcatct
    19141 aaaaattcac ttcccatttt gattcctaat atatcgttga ttctttcctc agcaatcgct
    19201 cttttctgct caacagtaat attatttaca cttccaacat ttaaaatatt gctctttcct
    19261 atgccatcca ttctattcaa ttcctttaaa catcctaatc tgtctttcat gtccttttct
    19321 tcgtcttgaa tgttatcact aagccattgt ctacgctgct ctactgtcat aacacttctt
    19381 tgatctctct tttttaccct ctcatgtatg acattcctaa ataaaggact gtttaatatc
    19441 ttatatccct tgttataagc actcttatcg cttaaatcag gacgaatctt ttgcatggac
    19501 ttcgtaatat taccactctt tgaatactcg tcaaagaatc tcttagcttc atcctcacgc
    19561 tttaattctg aaacactctt tgcccttggc atactctcat cctctctttc tctacctccc
    19621 tacattataa gttatttcaa tgcggacgtt ttccccccta taaaaaacac atgaactcat
    19681 ttttttcaaa actctaattt tcgttttcca aaaattttta tctaaaaaag ggggtggttt
    19741 tataattaat attatttttt tatttgatta gcactctgtt gtgtatagtg ctaggtgtaa
    19801 aaaatgtggt ttggtcgaga gggaaggcat ggggtgtgta tagtcgcttt ttcctgttgc
    19861 gattttcaaa tcatgacccc aaacgtatat atatttatgt atgcaatacg tgaacaacat
    19921 tcaaacatga tcatgtaaat agttttagca gcaagaagaa aaagcaagaa gaaaaagcaa
    19981 gaagaaaaag caagaagaaa aaagacaata aaaaagctag ttaaaaactt aatattttaa
    20041 ctagcataat agaattataa taataaatat tataataaat aatataataa taaataacca
    20101 gtattttaat ataaaagcag ctatgagaat taatacaagt atagttgtaa gctgatcaat
    20161 catctatcaa cctctttttt atccccacat agtgccaaat cgtatacact catttaatgg
    20221 cac
//
LOCUS       DBMDWL010000016        20188 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_947_length_20188_cov_3.643650, whole genome shotgun sequence.
ACCESSION   DBMDWL010000016 DBMDWL010000000
VERSION     DBMDWL010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20188)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_947_length_20188_cov_3.643650"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(142..1044)
                     /locus_tag="ACZPDN_02010"
     CDS             complement(142..1044)
                     /locus_tag="ACZPDN_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024748.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR1297432.1"
                     /translation="MDKKNKIVIISLLSILLIALCMFASEMSSTKKKAFTSESEIQDD
                     QSTEVKNEDNSKYIDMALAKEIEAYFQENNIDHESVSYCITDLKHNIKYSMNEKDEFI
                     AGSIYKLPLAMLYYDKVNSGDYTMESTLPYSGYMHEDAGVISSNYTIGSQIPLSELLD
                     AAIVYSDNDAGHILFENLGGWQEYKEAMTKYTSSISDNYYSEDNVSTANTMNDVITYL
                     YAHKDEYQELIDNMKKAEPQEYLDRDIQQGMPQKYGMYDNALNSVGFVECETPYSIVV
                     LTSLGDKGADVMAKINSIAYEHFK"
     gene            complement(1095..2627)
                     /gene="purH"
                     /locus_tag="ACZPDN_02015"
     CDS             complement(1095..2627)
                     /gene="purH"
                     /locus_tag="ACZPDN_02015"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR1297433.1"
                     /translation="MKRALVSVSDKTGLVEFVQGLVDCGYEIISTGGTKKALEAAGIH
                     PIGISDVTGFPEIMDGRVKTLHPKVHGALLCVRSNPDHVRQLQELGIEYIDLVCVNLY
                     PFKETVLKPGVTHEEIIENIDIGGPSMLRSASKNYQSIPVLCDPKDYDKVLEEIRENH
                     ETTLETRERLAAKVFRHTARYDAMIADYLTKKTHEEFPESMTITFDKVQDLRYGENPH
                     QKAAFYKGMNPQYSLANATQLHGKELSYNNIQDGNAAIEILKDFEGQFAAVGVKHMNP
                     CGVGIGENIEAAWDKAYEADSISIFGGIVALNAKVEKGLAEKLSKIFLEIIIAPDFSE
                     EALEILTRKKNIRLMKLDTSLSVSSALKYTNVNDGLLVQEMDQHIINEEDLKCVTNRK
                     PTEEEIKQLLFGWKVVKHVKSNAIVLVKNDMTVGVGAGQMNRVGAAKIAIEQAGEKAK
                     GSVLASDAFFPMPDTVQEAIKAGVTAIIQPGGSIKDQLSIDECNEHGIAMVFTGVRHF
                     KH"
     gene            complement(2624..3208)
                     /gene="purN"
                     /locus_tag="ACZPDN_02020"
     CDS             complement(2624..3208)
                     /gene="purN"
                     /locus_tag="ACZPDN_02020"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1297434.1"
                     /translation="MVNIAVFASGSGTNFETILSHIEDGSLHVNCACLIADKENAYAR
                     VRARNHGVEEFYFNPKGYDGKAGYEAAILEVLKEKKVDLIVLSGYMRFIGHTLLSAYP
                     NRIINLHPAYLPEFPGAHSIADAYEAKVAQTGVTVHFVDEGVDTGPIIRQERVAIDPS
                     WDLETLESHVHAMEYDLFWQVIEQVAKSIEEEKE"
     gene            complement(3202..4233)
                     /gene="purM"
                     /locus_tag="ACZPDN_02025"
     CDS             complement(3202..4233)
                     /gene="purM"
                     /locus_tag="ACZPDN_02025"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR1297435.1"
                     /translation="MAEHYAAAGVSLEAGYKSVELIKEHVKKTMRPEVLGGLGGFSGA
                     FSLSKIKDMEEPVLLSGTDGCGTKVKLAMVMNKHDTIGIDAVAMCVNDIACAGGEPLF
                     FLDYIACGKNVPEKIADIVKGVSEGCLQSEAALIGGETAEHPGLMPEDDYDLAGFAVG
                     VCDKKDMITGKNLNVGDVLIGLPSTGVHSNGFSLVRNVFDITEESLNTYYDELGKTLG
                     EALLAPTKIYVKALKHIKASGITLKACSHITGGGFYENVPRMLKDGMGVEIDTKAFPK
                     PPIFDLIQKNGDISTKEMYNVFNMGLGFVMAVDPSDVEKVQTALNEINEPSYVVGKVT
                     ETGQVDVQW"
     gene            complement(4237..5616)
                     /gene="purF"
                     /locus_tag="ACZPDN_02030"
     CDS             complement(4237..5616)
                     /gene="purF"
                     /locus_tag="ACZPDN_02030"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1297436.1"
                     /translation="MTMQKVVQDSSGIVGLFNVEQAAQNIYLAMHAIQHRGQDGVGVA
                     VSNGKNVVCKKGLGLLSENLKQDTLNSLAGDIAIGQLRMATKNDSQLENVQPIMVRSH
                     QRYFAVVSSGMVTNAVSLRTKLENEGLIFQGTSDSELLAHLIQLNPGSFEEKITKACR
                     MMSGAYTFMVITKDSLYVVRDPHGIRSLYIAKVNDGYCISSETCSFPILGGEFVREVN
                     PGELICFDRTGIKSSQIFEKTETKACALEYVYYSRPDSVHNGLTVHEVRKQCGYYLAK
                     EEDVKADIVVGVPDSALSAAASFARTLNVPYETGLIKNRYIGSTFIRPTEQQRMQGMR
                     VRLNAISSVVKDKSVYLVDDSVVKGFTSRRICQLLKEAGAKEVHLRIASPMLKYPCLY
                     GADSTAQKDLAAFNYSVDEMKQLFQVDSLRFISVEDFKKCVPETSCLACCTGEYPEEL
                     QDYKDEVKE"
     gene            complement(5618..9319)
                     /locus_tag="ACZPDN_02035"
     CDS             complement(5618..9319)
                     /locus_tag="ACZPDN_02035"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1297437.1"
                     /translation="MDTRIFVHKKEQFQVESESLAAELRQSLSLKEDFGLTKYNLYDI
                     FNADENDIELLKKNVCSEVVTDDVYDSVDLEGKSYLAYEFLPGQYDQRADSAMQCLML
                     LNNKQTVRIHSGTLLVFLNPVDEETLNKITHYIVNPVEARVKDLSVLVDDQDVEVKDV
                     PVLEGFTTKTKEELDSLRQTLGLAMSQADIEHVQRYFEKEEKRDCTMTELRVLDTYWS
                     DHCRHTTFETVLDDVHFHLDTLSDELQASYDKYLKLRQEVHGNRKEKTLMDMATIAGK
                     YLRKQGKLDDMEITDEINACSVEITVDVDGKDEQWLLMFKNETHNHPTEIEPFGGAST
                     CIGGAIRDPLSGRSYVYQAMRITGAGDITKPISETLEHKLPQSKISKTAAHGYSSYGN
                     QIGLATTFVDEIYDDGYVAKRMEVGAVVGAAPKSHVKREQPQAGDIIVMIGGATGRDG
                     IGGATGSSKEHNETSLEKCSSEVQKGNALIERKLQRLFRNPACTKLIKKANDFGAGGV
                     SVAVGELADGLDINLDAVPVKYQGLDGTELAISESQERMAVCIEAKDFEKFKEEAYKE
                     NLDAIKVADVTDTNRLVMKWRGETIVDMSRAFLDTNGVRQHQVVDVCENEYDEHPFDA
                     VNSDLNTVLQDANVASQIGLAEMFDASIGKSTVLMPFGGKYQLTPEEGSVQKLPVHGM
                     TNTCSVMTYGYDPKVSKYSPYLGASYSVVEALARITALGADYKKCRLSNQEYFEHLGA
                     DAQKWGQPFEALLGLIDAQLAFETPSIGGKDSMSGTYKDIHVPPTVITFAVTTAKTDN
                     IVSASFKNPGDYVYLVKHTPVKNHVPNYEQLKENFETVHKLMQEKKIVAASTIKFGGL
                     GATIAKMAFGSKVGVAINTEEDVYGFNLGSMIVEATESIQDEHLELIGILNDDSKIVL
                     NEETVEIEDALKAWTKRYSEIYPMIVDEGKETLETPLNDVEVPMSKVVVETPKVVIPI
                     FPGQNCEFDTTAKFERAGAEVKQVVFNNLTVENIQKSIETLAEEISTAQILMIVGGFS
                     SGDEPDGSGKFIANVLRNPKIAQAIDTMRANDGLILGICNGFQALIKSGLLPYGDIEK
                     LDEENPTLFRNNINRHVSHIARTRITSNASPWLAGMKAGNVYSVAVSHGEGKFVASEE
                     VLNKLIENGQVATQYVNEKGVPTMDGKDNLNGSSMAIEGIFSEDGKIFGKMGHSERYE
                     KGLFQNIAGNKDQDIFANGVRYFTHKQVK"
     gene            complement(9330..9818)
                     /gene="purE"
                     /locus_tag="ACZPDN_02040"
     CDS             complement(9330..9818)
                     /gene="purE"
                     /locus_tag="ACZPDN_02040"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1297438.1"
                     /translation="METKRVLIVMGSRSDLPSMEGCMKQLDDFQVGYEVRVLSAHRTP
                     EAVLKLSKTACERGIQVIIAAAGGAAHLAGVLASSTPLPVIGIPIQTSALGGLDSLLS
                     TVQMPQGVPVATVAIGKSGARNAAILACQMIGLNDKSLQDKIVEFKKEQAQKVLEQSI
                     VE"
     gene            complement(10005..11351)
                     /locus_tag="ACZPDN_02045"
     CDS             complement(10005..11351)
                     /locus_tag="ACZPDN_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297439.1"
                     /translation="MAKQDSVQLMTKGSIAKQIIGYAIPIFIGYLFQQLYNTVDALIV
                     GNFLGQDALAAVTSVGSLIFLFVGFFNGFATGASVIIANAIGAQDEDRTKKAVYTTAT
                     FGIILGLIMTVSGYVLTDQMLMWMGSPREVFGLSSTYLKIYFLGGFSLVLYNMLVGII
                     RAGGDAKHPLYYLIVSSILNVILDVVFITVFKMGVAGAAWATIISEFASMFLCAVQLA
                     REESILHISWTHLSLDFENLKQICIYGLPTGLQGCVIDFANVMIQSYINSFGAAAIAG
                     IGAYSKVEGFIFLPEISFSMALTTFVSQNEGAGQKERSRKGILFGLAASLLMIECMGI
                     LIYLFAPTFISFFNQNLEVIAIGVDRAKICAFFYVLLGFSHVVSSVLRGLGRPVMPMV
                     VMLVCWCAVRVVVLMTIGQSMHSLYLTHWLYPITWGLSSIVYIFDLRKYKLFTPRV"
     gene            complement(11443..13101)
                     /locus_tag="ACZPDN_02050"
     CDS             complement(11443..13101)
                     /locus_tag="ACZPDN_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1297440.1"
                     /translation="MYKKLLVCGLALLMCGCSTKAAGNDQVLTVGLSSEMNGTFSPMY
                     YQTTNDSYVVDLVYQGLLKYNKKGELVADLAKSLPKISEDGKTMTFKLKKGIKFSDGS
                     KFTSKDVQTTFTVMADPSYTGRFANNVDFLDGYSEYHDADASSFTGIETPDNYTVVFH
                     LDKPRIDAVSTLGTQKICSSEYLNYKKGKTESIEKKNSKPIGTGAYVLKKFEKTSGAS
                     LVSNSKFKAKKGQYQISKIMIKKTDTSTEVKELENGTVDYIPDVIEANKIKSVQKNKS
                     LSVDSYPSDRESFIIFNSYAGACSDVAIRKAIGYGFNAQEYIDNYYQIDGMAYKPGTF
                     GNPVSLNNGKMIRNEEKVDGVTYYDYDVEKANQILEDAGYTLAEDGYRYKDGEKLTIR
                     FLANKDKDSLDSLIPVLQKCLNAIGIDFKANTVEFNTVINTVQDDAQTDSWDATYLGF
                     SYGSPDDTGINFILRTGATNNFGRLSDEQLDAYLDAGMYTSDNEVSKENYHNALVRQS
                     ELCGYLPVDSTKTYCLRNKNIKGMHTTSIYNWAQSIATAYIVNK"
     gene            complement(13506..15923)
                     /locus_tag="ACZPDN_02055"
     CDS             complement(13506..15923)
                     /locus_tag="ACZPDN_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HIR1297441.1"
                     /translation="MENAEHFGTLTERMKEFREEVLDEKPYIDAQRAILATLAYKENL
                     NQPRVMVRAKMLEKVLDNMSIYIEDKSLLAGNQATKNRNAPIFPEYTMEFVINELDQF
                     EKRDRDVFYITEKTKEQLREIAPFWENNNLRARGEALLPEEVRVFMETGVFGMEGKLN
                     AGDAHLAVNYERILKDGLKGYEKRVKECKASLDLTDPDSIDKYCFYNAVLIVLKAVRN
                     FANRYSVLAKDLAEKELNQKRKIELLEISRICSKVPYEPAETFKEAVQSVWFIQLILQ
                     IESNGHSLSYGRFDQYMYPYYDRDIKNGTIKESEALELLTCLWIKTLTINKVRSQAHT
                     LSSAGSPMYQNVTIAGQTTDKKDAVNDLSFLVLKSVAQTRLTQPNLTVRYHKNINKHF
                     LDECVEVMRLGFGMPALNNDEIIIPSFMDWQVKEEDAYNYSAIGCVETAVPGKWGYRC
                     TGMSYINFPRMLLCTMNNGVDLTSNRRFTKGYGYFTEMESYEELLKAWDKTIREITRY
                     SVIVENVIDKASERDVPDILCSALTDDCIARGKTIKEGGAVYDFISGLQVGIANMADC
                     LAAIKKLVYEEKKITRQELWDAILDDFSSPKNKKIQEMLIREAPKYGNDDDYVDQLIV
                     EAYDSYIEEIEKYPNTRYNRGPIGGIRYAGTSSISANVGQGMSTMATPDGRNAFEPLA
                     EGCSPAHNSDKNGPTAVFKSVSKLRTNKITGGVLLNQKMTPQMLSTEENRQKLELLIK
                     TFFNRLHGYHVQYNIVSKETLIDAQKHPENHKDLIVRVAGYSAFFNVLSKKTQDDIIG
                     RTEQSLM"
     gene            16105..17019
                     /locus_tag="ACZPDN_02060"
     CDS             16105..17019
                     /locus_tag="ACZPDN_02060"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HIR1297442.1"
                     /translation="MTTTNRNITGIIFNIQKFSVNDGPGIRTTVFLKGCPLRCKWCAN
                     PESQLIQTQILWDQKKCINCHHCIETCPKNAMKIIDKSIKINHSLCSGCKKCVYECPK
                     TALSAQGEQKSVEEVLKIVLQDQAFYEESGGGLTLSGGELLLQPEFSRELLLAAKEEG
                     IHTCCETTGFATEETFDHVMEYVDYILFDMKHWNTDKHIEGTGVSNKLPLLNMKHAIA
                     NGKTVLPRIPVIPGFNDSLDDAKEFSKTLHNIGINKCQLLPFHQFGENKYHLLNIKYD
                     YENVPSYHPEDLKEYLNMFKENNINAFF"
     gene            17084..17830
                     /locus_tag="ACZPDN_02065"
     CDS             17084..17830
                     /locus_tag="ACZPDN_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1297443.1"
                     /translation="MKNRTNKILELLTSEKRIEVSQLAEYLGVSQVTIRKDLDALEAK
                     GIIKREHGHAVLCSTDDINGRIAYHYEEKKKIALKATELVHEGDTIMIESGSCCALLA
                     DTLTQLHKNLTIITNSAFIAEYIRGKSNFQIILLGGIYQQDSQVMVGPMVQECVSNFF
                     VDYFFIGTDGYDSRIGFSNRDQMRAQAVRDMSHQAEQIIVLTESDKFSKRGVVPLNLK
                     DKIKTVITDHSINSSYVQELNNQNIQVVTI"
     gene            complement(18312..19994)
                     /locus_tag="ACZPDN_02070"
     CDS             complement(18312..19994)
                     /locus_tag="ACZPDN_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1297444.1"
                     /translation="MNKRSVCALGMAFVLAGCSAGGSGSGKEQTLTVGLFTEMNGDFS
                     PMYYQTVNDHNVVNLVYQGLLAYDKDANLVPELAEDLPTISDDGTTMTFKLKKGVKFS
                     DGSKFTSKDVKATFSVMADPSYTGRFSSNVDFLNGYSEYHDGDAKDVSGIETPDDYTV
                     VFHMAKPKIDAESTLGIQKICSAKTLKYKKGKTSNVEKNNSNPIGTGPYKLKSFSKTE
                     GASLDRNENFEAKDGEYQISKIMIKKTETSTEIKELENGTVDYIPDVVEANKINTVAK
                     DKDKGLTMDSYPSDREDFLFMNTAAGACQDQAVRQALAYGFDREAYIASYYKLDDKDA
                     KLAYVPGLFGNPVSGGNGAYVRGEEKVDGATYYDYDVEKAKQILDDAGWTVGSDGIRE
                     KDGQKLSIKFLATKEKDAMDTMIPMLQKQWGELGVDFKANTMEFNTVIATVSDDSAVG
                     DWDVSYLGNSFTSPEDTSVDYFIQSQGVNNLARLKDDELDSYLAAGAYTADKDASAAA
                     YLKAYVRQAELCAYLPTDGVQTYCLRNKKVKGLKTSSTLIWSESMATAYIDD"
BASE COUNT         6312 a         4289 c         2737 g         6850 t
ORIGIN      
        1 aagatcatgt gcacctcagc agtgctcatc tatattacca aatacttccc ccttcgtcaa
       61 atgttttttt tacttttttt catctttttt actgtatacg cttacatcgc catttttaaa
      121 aaaataaggc ccccttcgac cttatttaaa atgttcatat gcaatgctat taatttttgc
      181 catgacatcg gcacctttat cacctaaact tgttaataca actatgctat atggtgtttc
      241 gcattctaca aagccgacac tattcaatgc attatcatac ataccatatt tttgaggcat
      301 accctgttga atatctcgat ctaaatactc ttgcggctct gcttttttca tattgtctat
      361 caattcttga tactcatcct tatgagcata taaataagta attacatcat tcattgtatt
      421 agcagttgaa acattgtcct ctgaataata attatcacta attgaacttg tatacttcgt
      481 catggcttct ttatattctt gccatccgcc tagattttca aacaatatat gacccgcatc
      541 attatccgaa taaacaattg ctgcatccaa taattcactt aatggtattt gacttccaat
      601 tgtataattt gaactaataa cacccgcatc ctcatgcata taaccactat atggtaaagt
      661 tgattccata gtataatcac cactattcac tttatcataa tataacatgg ccaaaggaag
      721 cttatatata ctacctgcaa taaactcatc cttttcattc atactgtatt ttatattatg
      781 tttcaaatct gtaatacaat acgacacact ctcatgatca atattatttt cttggaaata
      841 tgcttcaatt tcctttgcta aagccatatc tatatattta ctgttatctt cattttttac
      901 ttccgtactt tgatcatctt gaatctcact ctctgaagta aatgccttct tttttgtact
      961 actcatttca gaagcaaaca tacaaagtgc aattaataga atagataata aagaaatgat
     1021 tacaatttta tttttcttat ccataaacag acccttccaa actatgaaaa agagtctatt
     1081 cagactcttt tatattaatg tttaaaatga cgaactcctg taaataccat tgctatacca
     1141 tgttcattac actcatcaat tgaaagttga tctttaatcg atccacctgg ctgaatgatt
     1201 gcagtaacac ccgctttgat agcttcctgc acagtatctg gcattgggaa gaatgcatca
     1261 cttgctaaaa ctgaaccctt tgctttttct ccagcttgtt cgattgcaat ctttgcagca
     1321 ccgacacgat tcatctgacc agcaccaaca ccaactgtca tatcattttt taccaatacg
     1381 atcgcatttg attttacatg tttaacaact ttccatccaa acaacaattg tttgatttct
     1441 tcttcagttg gtttacgatt tgttacacat tttaaatctt cctcgttaat tatatgttga
     1501 tccatttctt gaaccaatag accatcgtta acatttgtat attttagcgc acttgaaaca
     1561 gataagctag tatctaattt cattaatcga atatttttct tacgagtcaa gatttccaat
     1621 gcttcctcag agaaatctgg tgcaatgata atctccaaga aaatcttaga taatttttct
     1681 gccaatccct tttcaacttt tgcgtttaac gcaacaatac caccaaaaat tgaaattgaa
     1741 tctgcttcat aagctttatc ccaagctgct tcaatatttt cgccaatacc aacaccacat
     1801 ggattcatgt gtttaacacc aacagccgca aattgacctt caaaatcctt tagaatttca
     1861 attgccgcat taccatcctg aatattgttg taagataatt ctttaccatg aagctgtgtt
     1921 gcatttgcca aagaatattg aggattcatg cccttataga aagcagcctt ttgatgaggg
     1981 ttttcaccat aacgcaagtc ttgaacctta tcaaaagtaa ttgtcatact ttctgggaat
     2041 tcttcatgtg ttttcttagt taaataatcc gcaatcatcg catcataacg agctgtatga
     2101 cggaatactt ttgcagctaa gcgctcacgc gtttctaatg ttgtttcatg attttctcta
     2161 atttcttcaa gaaccttatc ataatctttt ggatcacaaa gaactggaat actttgatag
     2221 tttttagaag cactacgaag catacttgga ccaccaatat caatattctc aataatttct
     2281 tcgtgtgtaa caccaggttt taacacagtt tctttgaaag gatataaatt cacacaaacc
     2341 aaatcaatat actcaatacc taattcttgt aattgacgaa catgatccgg gtttgaacga
     2401 acacaaagca atgcaccatg aacttttgga tgtaaagttt ttacacgacc atccataatt
     2461 tcaggaaaac cagtgacatc actaattcca attggatgga ttcctgctgc ttctaaagct
     2521 ttttttgttc ctccagtaga aataatttca tatccacaat ctactagccc ctgtacaaat
     2581 tcaactaaac ctgttttatc agatacgctc actaatgctc gcttcattct ttttcctcct
     2641 caatactttt tgcgacttgt tcaatgactt gccagaacaa atcatattcc atcgcatgta
     2701 catgcgattc taatgtttct aaatcccaag atggatcgat tgctactctt tcttgacgaa
     2761 tgataggtcc tgtatccacc ccttcgtcta cgaaatgaac agtaactccg gtttgtgcta
     2821 cttttgcctc atatgcatcc gcaatactat gagctcctgg aaattcaggt aaataagctg
     2881 gatgcaaatt gataatgcga tttggataag cactcaacaa agtgtgtcca atgaaacgca
     2941 tatatccact caatacaatc aaatctacct ttttttcttt taatacttct aaaatagccg
     3001 cttcataacc cgctttacca tcatatccct ttggattaaa gtaaaactct tctactccat
     3061 gattgcgcgc acgaactctt gcatacgcat tctctttatc agctatcaag caagcacaat
     3121 tcacatgtaa agaaccatct tcaatatgag ataagattgt ttcaaagtta gtgcccgaac
     3181 cactcgcaaa tactgcaata tttaccattg gacatctacc tgtcctgttt cagttacttt
     3241 accaacgaca taacttggtt cattgatttc atttaatgca gtttgaacct tttctacatc
     3301 cgacggatct actgccatca caaaaccaag tcccatattg aatacgttat acatttcttt
     3361 tgttgaaata tccccatttt tttgaatcaa atcaaagatt ggaggttttg ggaaagcttt
     3421 cgtatcaatt tcaactccca ttccatcctt taacatacga ggaacgtttt catagaatcc
     3481 accacccgta atgtggctgc aagcttttaa cgtgattcct gaagctttaa tatgctttaa
     3541 tgccttaaca taaatctttg taggtgcaag taacgcctct cctaaagttt tgcctaattc
     3601 atcataatat gtatttaatg attcttctgt aatatcaaat acattacgaa ccaatgagaa
     3661 accattactg tgaactccag ttgaaggaag tccaatcaag acatctccaa catttagatt
     3721 cttaccagta atcatatctt ttttatcaca aacaccaacc gcaaagccag ctagatcata
     3781 atcatcttct ggcatcaaac ctggatgctc tgccgtttct ccaccaatta aagcggcttc
     3841 agattgtaaa caaccctcag aaacaccttt aacaatatct gcaatttttt ctggaacatt
     3901 cttgccacat gcgatgtaat ccaagaagaa caatggttct cctccagcac atgcgatatc
     3961 attcacacac attgcgacag catcaatacc aattgtgtca tgcttattca taaccatcgc
     4021 aagtttaact tttgttccac aaccatccgt accagaaagc aacactggct cttccatatc
     4081 tttaatttta gataatgaaa acgcaccaga gaaaccacca agtcctccta atacttctgg
     4141 acgcattgtt ttcttaacat gctccttaat caattcaaca gatttatatc ctgcctctaa
     4201 actaacgcct gctgctgcat aatgttctgc catactctac tcctttacct catctttata
     4261 atcttgtaac tcttctggat attcacctgt acaacaagct aaacaacttg tttcaggtac
     4321 acatttcttg aaatcctcta cacttataaa acgtaacgaa tccacttgga acaactgttt
     4381 catttcatct accgaataat tgaaagcagc taaatctttt tgtgccgttg aatctgctcc
     4441 atataaacaa ggatatttca acattggcga agcaatacgt aaatgcactt cttttgcccc
     4501 tgcctctttt aatagctgac aaattctacg tgatgtaaat ccttttacaa cactatcatc
     4561 cactagatat acacttttat ctttaacaac tgaactgatc gcatttaaac gcacacgcat
     4621 accttgcatt ctttgttgct cagttggacg aataaatgta gaaccaatat agcggttttt
     4681 gattaatccc gtttcatacg gtacatttaa tgttctagca aagcttgcag ctgcgcttaa
     4741 agccgaatca ggaacaccaa caacaatatc cgccttaaca tcctcttctt tagctaaata
     4801 ataaccacat tgttttctta cttcatgaac cgttaaacca ttatgaacac tgtccggtct
     4861 agagtaatat acatattcta aagcacaagc tttagtttca gttttttcaa aaatttgact
     4921 tgatttaatt cctgtacgat caaaacaaat taattcacct ggatttactt cacgaacaaa
     4981 ttctccacca agaattggga acgaacaagt ttcactactg atacaatatc catcatttac
     5041 tttggcaata tataaagaac gaattccatg tggatctctt actacgtata aactatcttt
     5101 tgtgatgacc ataaatgtgt aggcaccact catcatgcga caagcctttg tgattttttc
     5161 ttcaaaagaa cccggattca attgaatcaa gtgtgctaac aattcagaat cacttgttcc
     5221 ctggaaaatt aagccttcat tctctaactt tgttcttaat gaaactgcat ttgtgaccat
     5281 accacttgat acgaccgcaa aatatctttg gtgactacgc accataatcg gttgaacatt
     5341 ttctaattga gaatcatttt ttgtagccat gcgaagttga ccaatcgcta tatcaccagc
     5401 taaagaattc aatgtatctt gttttaaatt ctcagacaac aatcctaatc cctttttaca
     5461 aacaacgttt ttcccattac tcacagccac accaacacca tcttgtccac gatgctgaat
     5521 ggcatgcatt gctaaataga tgttctgggc ggcttgttca acgttgaaca agcctacgat
     5581 cccagaagaa tcttgaacta ccttttgcat cgtcattcta cttcacctgc ttgtgtgtaa
     5641 agtaacgaac accatttgcg aaaatatcct gatctttatt tcctgcaata ttttggaata
     5701 agcctttttc ataacgctca ctatgaccca tcttaccaaa gatttttcca tcctcagaga
     5761 aaataccttc aattgccata cttgaaccat tcaagttgtc ttttccatcc attgttggaa
     5821 cacccttttc atttacatat tgtgtcgcaa cctgaccatt ttcaatcaat ttatttaaca
     5881 cttcttcact cgcaacaaat ttaccttcac catgactaac agccactgaa tatacatttc
     5941 ctgctttcat gccagcaagc catggagatg cattgcttgt aatgcgtgta cgtgcaatat
     6001 gcgaaacgtg acggttaatg ttatttctga ataatgttgg attttcttca tccaattttt
     6061 caatatcacc ataaggcaac aatccagatt taatcaatgc ttggaatcca ttacagatac
     6121 ctaaaatcaa tccatcatta gcgcgcattg tatcaatagc ttgggcaatc tttggatttc
     6181 ttaatacgtt cgcaataaat ttacctgaac catctggttc atcaccacta ctaaatccac
     6241 ctacaatcat taaaatttga gctgtactga tttcttcagc caatgtttca atactctttt
     6301 gaatattttc aactgttaaa ttattaaata cgacttgttt tacttcagca ccagcacgtt
     6361 caaacttagc tgttgtatca aattcacagt tttgtccagg gaaaatagga ataactacct
     6421 ttggtgtttc cactacaacc ttagacattg gtacttccac atcatttaaa ggagtttcta
     6481 acgtttcttt tccttcatct acaatcattg gataaatttc gctataacgt tttgtccaag
     6541 cctttaatgc atcttcaatt tcgactgttt cttcatttaa tacaatctta gaatcatcat
     6601 ttaaaatacc aattaattct aaatgctcat cttgaatgct ttctgtagct tccacaatca
     6661 ttgagcctaa attaaatccg tatacatctt cttctgtatt gattgcaaca ccgactttag
     6721 atccaaacgc catcttcgct atagttgcac ctaatccacc aaatttaatt gtgcttgccg
     6781 ctacaatttt cttttcttgc atcaatttat gaaccgtttc aaaattttct ttcaattgtt
     6841 catagtttgg aacatgattc ttaactggcg tatgttttac caaatataca tagtctccag
     6901 gatttttaaa tgaagcagat acgatattat ctgttttcgc tgtagttacc gcaaatgtga
     6961 ttactgttgg tggtacatga atatctttat acgttccact catagagtct tttccaccaa
     7021 tactaggcgt ttcaaatgcc aattgtgcat cgatcaaccc taataaagct tcaaatggtt
     7081 gtccccattt ttgtgcatct gcacctaagt gctcaaaata ttcctgattt gataaacgac
     7141 acttcttata gtcagcacct aacgctgtaa tacgagctaa tgcttcaaca actgaataac
     7201 tagcccctaa atatggagaa tatttagata cttttggatc atatccatat gtcataacag
     7261 aacaagtgtt tgtcattcca tgaactggta atttttgtac agaaccttct tctggcgtta
     7321 attgatattt acctccaaaa ggcatcaata ccgtactctt tccgattgaa gcatcaaaca
     7381 tttctgccaa accaatctga cttgcgacat tcgcatcttg taatactgta tttaaatctg
     7441 aatttactgc atcaaatgga tgttcatcat attcattttc gcaaacatca acaacctggt
     7501 gttgacggac accatttgta tccaagaaag cacgtgacat atccacaatc gtttcaccac
     7561 gccatttcat gactaaacga tttgtatctg taacatccgc aactttgata gcatctaaat
     7621 tttccttata tgcttcttct ttgaattttt caaaatcttt agcctcaata catacagcca
     7681 tacgttcttg ggattcacta atagctaatt ctgtaccatc taaaccttga tatttaactg
     7741 gaactgcatc taagttaata tccaatccat ccgcaagttc acctacggca acactaactc
     7801 cgccagctcc aaagtcattg gcctttttaa ttaattttgt acaagcagga tttctaaata
     7861 aacgctgtaa tttacgttca attaaggcat tacccttttg aacttcactt gaacattttt
     7921 ccaagcttgt ttcattatgt tctttactag aacctgtggc tccaccaatt ccatcacgac
     7981 cagttgctcc accaatcata acgatgatat cgcctgcttg tggttgttca cgttttacat
     8041 gtgatttagg agctgcacca acaaccgcac caacttccat acgttttgca acatagccat
     8101 catcataaat ttcatctaca aatgtagttg ccaaaccaat ttggttacca taacttgaat
     8161 aaccatgagc cgctgtcttt gaaatctttg attgaggtaa tttgtgctct aatgtttcac
     8221 taattggttt cgtgatgtct ccagcccctg taatacgcat agcttgatat acataactac
     8281 gtcctgataa tgggtcacga atagctccac caatacatgt acttgctcca ccaaatggtt
     8341 caatttctgt aggatggtta tgtgtttcgt ttttgaacat caacaaccat tgttcatctt
     8401 ttccatctac atccactgta atctctacac tacatgcatt gatttcatcg gtaatctcca
     8461 tatcatcaag cttaccttgt ttacgaagat attttccagc aatagtagcc atatccatca
     8521 atgttttttc tttacgattt ccatgaactt cttgacgtaa ttttaaatat ttatcataag
     8581 aagcctgtaa ttcatcggat aatgtatcca aatggaaatg tacatcatct aatacagttt
     8641 caaaagtagt atgacggcag tgatctgacc aatatgtgtc tagcacacga agttcagtca
     8701 ttgtgcaatc tcttttttct tccttttcaa aataacgttg aacatgctca atgtccgctt
     8761 gagacatggc caaccctaaa gtctgacgta aagaatctaa ttcttctttc gtctttgttg
     8821 taaatccttc taaaacagga acatctttta cttcaacgtc ctgatcatct accaatacag
     8881 aaaggtcttt aacacgtgct tcaacagggt taacaatata atgtgtaatt ttattaagtg
     8941 tttcttcatc tactgggttt aaaaacacta acaaagtacc tgaatgaata cgtactgttt
     9001 gtttgttgtt caaaagcatt aaacactgca ttgcactgtc tgctctttga tcatattgac
     9061 ctggcaaaaa ttcatatgcc aaatatgatt taccttctaa gtctacagaa tcatatacat
     9121 catctgtaac aacttcacta catacatttt tctttaatag ttcaatatca ttttcatcag
     9181 cgttaaaaat atcatataaa ttgtattttg ttagaccgaa atcttctttt aaagataaag
     9241 attgtcttaa ttcagccgct aatgattcac tttcaacctg aaactgttct tttttatgaa
     9301 caaaaatacg agtatccatt tccttctcct tattcaacaa ttgactgttc taatactttt
     9361 tgtgcttgtt cttttttaaa ttcaacaatc ttatcctgta atgacttatc attaagtcca
     9421 atcatctgac aagctaagat agctgcattt cttgcccccg atttaccaat tgctacagtc
     9481 gcaacaggaa ctccttgagg catttgtact gttgataata atgaatccaa tccacctaaa
     9541 gcagatgtct gaatagggat tccaataact ggcaacggtg tagagctagc taaaactcca
     9601 gctaaatgtg ctgctcctcc agctgccgca ataataacct gaattccacg ttcgcacgct
     9661 gtctttgaca attttaaaac agcttctgga gtacggtgag cacttaatac acgaacttca
     9721 tatccgactt gaaaatcgtc taattgtttc atgcagcctt ccatactagg taagtctgaa
     9781 cgagaaccca tcacaattaa aacacgcttt gtttccataa atgaccttcc tttcaaaaca
     9841 aaaaagcgca cctatagtag gcacgcttaa aaaagcaatt cttctctatc aattgtggta
     9901 aatcttctta agaccataag taagacctcc aaaatcagaa aaacacttct gcaaaattat
     9961 aatacctaaa atcaactcaa atgacaaccc atatttcatg aaagttaaac tctaggtgtg
    10021 aataatttat attttcttag atcaaatata tagacaatcg agcttaatcc ccacgtaatt
    10081 ggatatagcc aatgcgttaa atacaaactg tgcatgctct gtccaattgt cattaaaaca
    10141 acaacacgaa cagcacacca acatactagc atcactacca ttggcatcac cggacgtcct
    10201 aggcctctta aaacacttga aactacatgt gaaaagccta aaagaacata gaagaaggcg
    10261 caaattttag cacgatctac tccaatcgca atcacttcca gattctggtt aaagaaagat
    10321 ataaaagttg gcgcaaacaa ataaatcaat atacccatac actcaatcat aagtagtgat
    10381 gctgcaagcc caaacaagat tccttttcta gaacgttcct tttgtccggc tccttcattt
    10441 tgtgagacaa atgtcgttag tgccatcgaa aaagaaattt ctggaaggaa aataaatcct
    10501 tctacttttg aatatgcacc aatacctgca atagctgctg caccaaacga attaatatac
    10561 gactggatca tcacattcgc aaaatcaatg acacaacctt gtagtcctgt tggcaagccg
    10621 taaatacaga tttgtttaag attttcaaaa tccaaagaca aatgtgtcca agaaatatgt
    10681 aaaatacttt cttctcttgc caattgcaca gcacacaaaa acatactcgc aaattcagaa
    10741 atgattgttg cccaggcagc accggcaacc cccatcttaa ataccgtaat gaagacaaca
    10801 tctaaaatca cattcaatat tgaacttaca atcaaataat acagtggatg tttggcatcg
    10861 cctccagcac gaatgattcc cacaagcatg ttatacaaga ctaaagaaaa accgcctaaa
    10921 aaataaattt ttaaatatgt ggaagataag ccaaacactt ctcgaggaga tcccatccac
    10981 atcaacattt gatctgttaa tacatatcca ctcaccgtca taataagtcc taagataatc
    11041 ccgaatgtag cagttgtata taccgctttt tttgttctat cctcatcttg agcacctatc
    11101 gcattggcaa taatgacact cgctcctgtc gcaaaaccat tgaaaaaacc aacaaacaaa
    11161 aaaattaatg agccaacact tgtaacagca gccaacgcat cctgtcctag aaaattcccc
    11221 acaatcaatg catccacagt attgtatagt tgctgaaata gatatccaat aaatataggt
    11281 atcgcatagc caatgatttg tttggctata cttccttttg tcattaactg aactgaatct
    11341 tgttttgcca taaaaccctc cccatacaag aaaagcctgc accaacatta agttagtaca
    11401 ggactatatt ttgccctaat atccattagg atacatcata cgttatttat ttacaatata
    11461 ggctgttgca atggattgag cccagttata aatagatgtt gtatgcattc ctttgatatt
    11521 cttgtttctt aaacaatatg ttttagtaga gtctacaggt aaataaccac acaattcact
    11581 ttgacgaact aaagcattat gataattctc ttttgacact tcattatcgg atgtatacat
    11641 accggcatct aaataagcat cgagttgttc atcactcaaa cgaccaaagt tatttgtagc
    11701 acccgttctt aagataaagt taatacccgt atcatctggt gagccataag aaaatccaag
    11761 atatgttgca tcccagctat ctgtttgtgc atcatcttgc actgtgttaa taaccgtatt
    11821 aaattcaacg gtattggctt taaaatcaat tccaatcgca tttaaacact tctgtaaaac
    11881 cggaatcaaa gaatccaaag aatctttatc cttatttgcc aagaaacgaa tggttaattt
    11941 ttcaccatct ttataacgat atccatcctc tgccaatgta tatcctgcat cctctaagat
    12001 ttggtttgcc ttttcaacat cataatcata ataagtgaca ccatccactt tttcttcatt
    12061 acgaatcatt tttccattat ttaaagaaac aggatttcca aaagtaccag gcttataggc
    12121 catgccatca atttgatagt aattatcaat atattcttgg gcattaaatc catatccgat
    12181 ggcttttctt atggcaacat cactgcaagc tcctgcataa gaattaaaga taataaagct
    12241 ttctcgatca cttggataag aatcaacaga taaactttta tttttttgta cagattttat
    12301 tttatttgcc tcaatcacat ctggaatata atccactgtt ccattttcca attctttaac
    12361 ttctgttgaa gtatctgtct ttttgatcat gatcttagaa atttgatact gtcctttttt
    12421 cgccttaaac tttgaatttg aaactaaact agctccactt gtcttctcaa attttttcaa
    12481 tacataagca cccgttccaa ttggcttact attcttcttt tcaatacttt ctgtttttcc
    12541 ttttttataa tttaaatatt ctgaactaca aatcttctgt gttccaagtg tagaaactgc
    12601 atcgattcta ggtttatcca aatggaaaac aactgtatag ttatctggtg tttcaattcc
    12661 agtaaagcta gacgcatctg catcgtgata ttcgctataa ccatctaaaa aatcaacatt
    12721 gttcgcaaaa cgacctgtat aacttggatc tgccataacg gtaaatgttg tctgcacatc
    12781 ttttgaagta aacttggatc catcactaaa cttaatgcct ttctttaatt taaatgtcat
    12841 tgtctttcca tcttcactaa tttttggtaa ggatttagcc aaatctgcaa ctaattctcc
    12901 ttttttgttg tatttcaata acccttgata cactaaatcc accacataag aatcatttgt
    12961 cgtttgataa tacataggtg aaaatgtgcc attcatttcc gaacttaatc cgactgtcaa
    13021 cacttgatca ttacctgcag cttttgtact gcacccacac atcaataaag ccaatccaca
    13081 tactaataac tttttgtaca tattctctta cctcctaatt atttattttt tgtaccacca
    13141 ccagtcagct aacacttttc atcactctcc ttataaataa aaaatcctgc atcaaattaa
    13201 aaatttgaca caggacagaa tatcaaaata atctgcggta ccacctgaat tggtatagtt
    13261 atacccacct ctagaatgcc atcacattcg atccatttaa cgcatggaat acgtcgcagc
    13321 tactcagaaa atcttttcac cttgcccttg aaagtccatt tgctaagtcg taataatcta
    13381 gcttccacca tgactagaat ctctttgtat ccggttctta gtttaatctc tttcgcatcg
    13441 gttgcctata gaatacacct aataaaattt attttcaacc atttttacaa attatttaca
    13501 ttttcttaca ttaaagattg ttctgttcta ccaataatat catcttgcgt ctttttagaa
    13561 agcacattaa agaaagcact atatccggct acacgaacaa ttaaatcctt atggttttcg
    13621 ggatgcttct gtgcatcaat caaagtttct ttagaaacaa tgttatattg cacgtgatat
    13681 ccatgcaagc gattaaagaa agttttaatc agcaattcta atttttgtcg attttcttca
    13741 gtcgacaaca tttgaggtgt cattttttga tttagcagaa ctccacctgt aattttattt
    13801 gtacgaagct tagacacact tttaaacaca gctgtaggtc catttttatc gctgttatgc
    13861 gctggactgc aaccttctgc caaaggttcg aatgcatttc gtccatccgg cgtagccatc
    13921 gttgacatac cttgtccaac attcgcacta attgaagaag ttcctgcata acgaattccg
    13981 ccaattggtc ctctattata gcgtgtattc ggatattttt cgatttcttc aatataagaa
    14041 tcataagcct ccacaattaa ctgatcgaca taatcatcat catttccata ttttggtgct
    14101 tcacgaatca acatttcctg aatttttttg tttttaggtg atgaaaaatc atctaagatt
    14161 gcatcccaaa gctcttgtct tgtgattttc ttttcttcat acactaactt ctttatagct
    14221 gccaaacaat ctgccatgtt cgcaattcct acttgaagtc ctgaaataaa atcatagact
    14281 gctccacctt ctttgatggt tttgcctctg gcaatacaat catctgtcaa agccgaacaa
    14341 agaatgtctg gcacatctct ttcagaggcc ttatcaatta cattttctac aataacacta
    14401 tatcgagtaa tttcccttat tgttttatcc catgctttta aaagttcttc ataactttcc
    14461 atttccgtaa aatagccata tccttttgta aaacgtctat tacttgtaag atccacaccg
    14521 ttattcatcg tacacaaaag cattcttgga aaattgatat aagacattcc cgtacaacga
    14581 taaccccatt tacctggaac tgctgtttct acacaaccta tggcactata attataggca
    14641 tcctcctctt taacttgcca atccataaag gaaggaatta taatttcgtc attgtttaat
    14701 gctggcattc caaagcctaa acgcataacc tctacacatt catccaaaaa atgtttattg
    14761 atattcttgt gatagcgaac agttaaattt ggttgtgtca atcttgtctg tgcaacagat
    14821 ttcaaaacta aaaaactcaa atcattgact gcatcctttt tatctgttgt ttgacctgca
    14881 atcgttacat tttgatacat aggagatcct gccgaactta gcgtatgtgc ttgagaacga
    14941 actttattga ttgtaagtgt ctttatccac aagcatgtca acaattccaa ggcttcagat
    15001 tctttaatag tgccattttt aatatctcga tcataatatg gatacatata ttgatcgaat
    15061 ctgccataac tcaaagaatg tccattggat tcaatttgta aaatcaattg aataaaccat
    15121 acggattgta ccgcttcttt aaatgtttct gctggttcat atggaacttt tgaacaaatt
    15181 ctactaatct ccaataattc aatttttctt ttttgattca attctttttc tgctaaatcc
    15241 tttgcaagaa cgctgtaacg atttgcaaaa ttacgcaccg ctttcagtac tataagaact
    15301 gcattataga aacaatactt atcaatacta tcaggatccg tcaaatcaag agatgcttta
    15361 cattctttta cacgcttttc gtatcctttt aatccatcct tcaagatgcg ctcatagttc
    15421 actgccaaat gcgcatctcc tgcatttaac ttaccttcca ttccaaatac accggtttcc
    15481 ataaagacac gaacttcttc aggaagaagt gcttctcctc ttgcacgtaa attattgttt
    15541 tcccagaatg gtgcaatttc tctaagttgt tccttagtct tctctgtgat atagaagacg
    15601 tccctatctc ttttttcaaa ctgatccaat tcgttgatca caaattccat agtatactca
    15661 ggaaaaatag gagcattacg gttcttggtt gcctgatttc ctgctaaaag agatttgtct
    15721 tcaatatata tactcatatt atcgagaact ttttctagca ttttagctcg aaccatcact
    15781 cgtggttgat ttaaattttc tttgtaagca agagttgcta gaattgcacg ttgtgcatca
    15841 atataaggtt tttcatctaa aacttcttct cgaaattctt tcattcgttc tgtcaatgtt
    15901 ccaaaatgtt ctgcattttc catatccgtc ctcccattat atttcattcg taactttata
    15961 ttcttttatt cgcaatttaa aaatagcatt acacgcattt catgtcaact ttaatttcat
    16021 ttaaaatatt ttatactttc atttgtaact ataatcattg cttttcttct ttgaaatgtt
    16081 tatactctat atgaggaaat taaaatgaca acaacaaatc gaaatataac aggaattatt
    16141 tttaacattc aaaaattcag tgtaaatgac ggtccaggca ttcgtactac cgtttttcta
    16201 aaaggatgtc ctcttcgttg taagtggtgt gcaaatcctg aaagtcagct tatacaaaca
    16261 caaattcttt gggatcaaaa aaaatgtata aattgtcatc attgcattga aacctgtcca
    16321 aaaaacgcta tgaaaataat tgataaatcc attaaaatca accatagtct gtgtagtgga
    16381 tgtaaaaaat gtgtatatga atgtccaaaa accgcattaa gtgcacaagg cgaacaaaaa
    16441 tctgtggaag aagtgcttaa aattgtttta caagatcaag ccttctatga agaaagtggt
    16501 ggtggtctaa cactttctgg aggtgagctt ttacttcaac ctgagttttc aagagaacta
    16561 ctcttagccg ctaaagaaga aggaattcat acatgttgtg aaacaaccgg ttttgcgaca
    16621 gaggaaacat ttgatcacgt catggaatac gtagattaca ttctttttga tatgaaacac
    16681 tggaacacag ataaacatat agaaggtaca ggtgtttcta ataagttacc attactgaac
    16741 atgaaacatg ccatcgccaa tggcaaaaca gttcttcctc gaatccctgt tatacctggt
    16801 tttaatgatt ctttggatga cgcaaaagaa ttttcaaaaa cacttcacaa cattggtata
    16861 aacaagtgtc aacttctacc gttccatcaa tttggagaaa ataaatatca tcttttaaat
    16921 ataaaatacg attatgaaaa tgttccttca tatcatccag aagatttaaa agaatattta
    16981 aatatgttta aagaaaataa cattaacgct tttttctaaa tttcactttc atttgaaatc
    17041 aaaaacactt attatataaa caaaatgata ggaatcaacg taaatgaaaa atagaacaaa
    17101 taaaatatta gaattattga catctgaaaa gagaatcgag gtttctcaac ttgctgaata
    17161 cctaggtgtt tcccaagtaa ctatccgcaa agatttggat gcattagaag ctaaaggaat
    17221 catcaagcgt gaacatggac atgctgtgtt atgtagcaca gatgatatca acggtagaat
    17281 cgcgtatcat tatgaagaaa agaaaaaaat agctttaaaa gctactgaac ttgttcatga
    17341 aggcgataca attatgattg aaagtggtag ttgttgcgca cttttagcgg atacgttaac
    17401 tcaacttcat aaaaacttaa ccattatcac aaacagtgct tttatcgcag aatatattcg
    17461 tggaaaatca aattttcaaa ttattctttt aggtggaatt tatcaacaag attcacaagt
    17521 tatggtaggt ccaatggttc aagaatgtgt atccaacttt tttgtcgatt attttttcat
    17581 cggcacagat ggatatgatt ctagaattgg cttttcaaat cgtgatcaaa tgcgagcgca
    17641 agccgtacgt gatatgtcgc atcaagccga acaaataatc gtgctaacag aaagcgataa
    17701 attttctaaa cgtggtgttg taccattaaa tttaaaagat aaaataaaaa ctgttataac
    17761 ggatcattct attaattctt cttatgttca agaactaaac aatcaaaata ttcaagtcgt
    17821 gacgatttaa aaaggagcca taacgaataa gtaattttta ttacttatgg agtttagttc
    17881 ctttcttttt atcttattat ttttttaact cctagagcct tagtttaaat tattgttttt
    17941 ctaaaattct agttttcgag tgtgatatct tcttagatta tgaccaacac atacaagtaa
    18001 tatctttagt ttttatcata atgattgtct tctttgatct gttcgaaaat cccttcactt
    18061 cagatacttt gttgtgtcat atatctttta gcctgctcag tcgaaaaatt ttctttggct
    18121 tcattttaat agaattcaag ctttcatatc ctgcaatacc agaatatgga atgatatctg
    18181 atatgcatta caattaacta gctatttttg cacaaacaaa agaaagaccg atattcagat
    18241 ttttttaaat cttgaatatt cggtcctttt ttaaactaaa acgtttttat ctgttatagc
    18301 cccttttact attaatcatc aatataagct gtagccattg attctgacca aataagtgta
    18361 gaagaagtct tcaaaccttt tactttctta ttacgtaaac aatatgtttg tacaccatct
    18421 gttggtaagt atgcacatag ttccgcttga cgaacataag cctttaagta agcagctgct
    18481 gaagcatctt tatcagctgt ataagcacct gcagctaagt atgaatctaa ttcatcatct
    18541 tttaaacgag caaggttatt tacgccttgt gattgaataa agtaatcaac gcttgtatct
    18601 tcagggcttg taaaagagtt tcctaaataa gatacatccc aatcaccaac agctgaatca
    18661 tcacttacag ttgcaataac tgtattaaat tccattgtgt ttgccttgaa gtcgacacca
    18721 agttctcccc attgtttttg taacatagga atcattgtat ccatcgcatc tttttctttt
    18781 gtagccaaga acttaatgct taatttttga ccatcttttt cacgaatacc atcactacca
    18841 acagtccatc ccgcatcatc taagatttgt ttagcttttt ctacatcata atcatagtaa
    18901 gtagcaccat ctactttttc ttcaccacga acatatgcac catttccgcc agatactggg
    18961 ttaccaaaca aacctggtac atatgctaat ttagcatctt tatcatctaa tttataatag
    19021 cttgcgatat aggcttcacg atcaaatcca taagccaaag cttgacgaac agcctgatct
    19081 tggcatgccc ctgctgctgt attcataaac aagaagtctt cacgatcact tggatacgaa
    19141 tccatagtca aacctttatc tttgtctttg gctactgtgt tgattttatt cgcttccact
    19201 acatctggaa tataatcgac agttccattt tccaattctt tgatttcagt cgaagtctct
    19261 gttttcttga tcataatctt agaaatttgg tattctccat ccttagcttc aaaattttca
    19321 tttcgatcca atgaagctcc ctctgtttta gagaatgatt ttaatttata aggacctgtt
    19381 ccgattgggt tactattgtt cttttcaaca tttgaagttt ttcccttctt atacttcaat
    19441 gtttttgcac tacaaatttt ttgtattccc aaagtcgatt ctgcatcaat ttttggcttt
    19501 gccatatgga atactactgt ataatcatcg ggtgtttcaa tacctgagac atctttggca
    19561 tctccatcat gatattcgct atatccattt aagaaatcaa cattacttga aaaacgtcct
    19621 gtataacttg ggtcagccat aacgctaaac gttgccttta catcttttga agtaaattta
    19681 gatccatcac tgaatttaac acctttcttc aatttaaatg tcattgttgt accatcatca
    19741 ctaatagtag gtaaatcctc tgccaattcg ggaactaaat tggcatcctt atcatatgcc
    19801 aacaatcctt gatataccaa gttaactaca ttgtgatcgt taacggtctg ataatacata
    19861 ggagaaaaat ctccattcat ttctgtaaac aatccaactg ttaatgtttg ttctttacca
    19921 gaacccgatc cacctgcaga acatcctgca agtacaaatg ccatacctaa ggcacataca
    19981 cttctcttat tcataactct tcctcttctc tttcatgtaa accattataa tgctttctga
    20041 aacatattca attgtttttt acatttaatt tctgtaacaa cattatttcc ttaactttgc
    20101 agaatttgta taactaaaaa ataaaagaag cattagtagc tgaattcacc aatgcttctg
    20161 tcaatatatt tttcatccat atcaaatt
//
LOCUS       DBMDWL010000017        19960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_961_length_19960_cov_3.247847, whole genome shotgun sequence.
ACCESSION   DBMDWL010000017 DBMDWL010000000
VERSION     DBMDWL010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 19960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19960
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_19960_cov_3.247847"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="ACZPDN_02075"
     CDS             <1..159
                     /locus_tag="ACZPDN_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HIR1297445.1"
                     /translation="VEVDEEGTTALQKLFNEIGPAYADRNGGYTRVVKTRIRRGDAAP
                     MAIVQFVK"
     gene            complement(200..>808)
                     /locus_tag="ACZPDN_02080"
     CDS             complement(200..>808)
                     /locus_tag="ACZPDN_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1297446.1"
                     /translation="KLALSDHRSSHITEDELIRLASRIRTASLIAGKQANLTIHMGDE
                     KQALELVFKALEKADIPISLFQPTHCTRNPHLFEQAIRFTEMGGTIDITCDGNGKTLN
                     YIRKIKNTEKVTISSDAQGSWSTYNEDGSVKEIGITPISNLRNEFIHLKNELGYENAL
                     PFFTKNVANVLGFQHTGQVKEGFDCDLVIWKEDQIQKVIVKK"
     assembly_gap    809..818
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<819..1253)
                     /locus_tag="ACZPDN_02085"
     CDS             complement(<819..1253)
                     /locus_tag="ACZPDN_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1297447.1"
                     /translation="MKLIKNIQIYAPKNLGVKDVLISDSKIEKIDDHIEISYPIETID
                     GTGCILTPGLIDRHVHITGGGGEAGFVSRARPIDVQEILDAGITTVIGLLGTDGYTRS
                     TKELFAKAKELTQKGVHTFILTGSYAYPSNTLTSSIEDDIVFV"
     gene            1300..3042
                     /locus_tag="ACZPDN_02090"
     CDS             1300..3042
                     /locus_tag="ACZPDN_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1297448.1"
                     /translation="MLISTESLKKNINDKCILKDASFTIEDTDKIALIGVNGTGKSTL
                     LKILAGIENYDSGTIIKKNGIKIHYLPQNPEFKTDCVWDEIQLVNSKNEHPVAEFELK
                     SILTKLGITDFQSVMSTMSGGQRKRVALALALCTSCDLLLLDEPTNHLDNDMVEWLEN
                     YLIRSKSAIFMVTHDRYFLDRVCTKMIELDQGDLYIHNGNYEVYLENKETRIEQEKLA
                     AHKHKNLYKKELEWVRAGVQARGTKSNSRLQRFEELRQKRFKQQEESLKINAAMQRLG
                     NKTIECMNLGFDYPEKQLFHDFNYVLLRHDRIGIIGENGCGKSTLLDVIAGLKQPTFG
                     TIEYGTTVKIGYFRQADQGVDLNMRVIDYIEEVSKVIEYDRMTLSASQMLERFLFPRN
                     MHYTTLSRLSGGERRRLYLLRVLMQEPNILLLDEPTNDLDILTLDVLEDYLDNFQGAI
                     ITVSHDRYFLDRVVDKVFVHQEDGTFKQYSGGYSDYLVKSKDENKKVVIQKPIEKKKR
                     SLLSYMEKKELEQLPEKMELLETEIEDLDHQMNECQSDFAKLKELSDLRTQKEEELEI
                     LTNRWMELEEKKEG"
     gene            3057..3620
                     /locus_tag="ACZPDN_02095"
     CDS             3057..3620
                     /locus_tag="ACZPDN_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1297449.1"
                     /translation="MENRVFFKKPSHVFLLAMLCCILWGSAAPFIKWGYQLFNIEGVP
                     SILMFAGIRFALAGLLVILFGSFLQKKVLVAKKENIQGICTLALFQTAGQYFFYYIGL
                     SHTSGVNGSIITGTSAFISLLVASLLFHYEKLTLRKTWGCFIGFLGILFMNMSGASAN
                     FSFIGEGFVFMSQLCGAISAAFIKKFF"
     gene            3639..3977
                     /locus_tag="ACZPDN_02100"
                     /pseudo
     CDS             3639..3977
                     /locus_tag="ACZPDN_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(4016..4792)
                     /locus_tag="ACZPDN_02105"
     CDS             complement(4016..4792)
                     /locus_tag="ACZPDN_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1297450.1"
                     /translation="MIDAHTHLENGDLSIEYVCKFIDAAKEKGITHLQILDHTHRFFE
                     FKDCYKRVCEVSKLQEEWFLKKQKNSIDDYLKLIAALKKETFDIDVRFGLEVCYTPET
                     ESLLSSILEHYQFDFLIGSVHSVNGYLYDMDAFSKELLWDKNDPSTIYKSYYDSIEKL
                     IQSNLFDQVGHPDVIKLYSIDPGYDLHPTYHHIARLANEYNIKMEDNTKAHYSYHHPD
                     IGLNADFRNILKENNVQIVTASDAHYPSDVGRCFELLDPR"
     gene            complement(4802..5950)
                     /locus_tag="ACZPDN_02110"
     CDS             complement(4802..5950)
                     /locus_tag="ACZPDN_02110"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HIR1297451.1"
                     /translation="MPKIKLYNNIAQIGLDQFTPDYEVNENMEDEDAILVRSANLHDI
                     EYNANLKAIARAGAGVNNIDIDTCTKKGIAVFNTPGANSNAVKELVFAGMLLASRDIY
                     HGIEWIQTQKENENIAKDVEKQKKKYAGHELTGKSLGVIGCGAIGIQVANLALRFGMK
                     VYGYDPYMSVDAAWNLSRYVNHVTNIDDLYRQCDFITIHVPLNDGTKSFINKDSFDKM
                     KDGVKLLNFARGGLVDEDALLEALNENKVASYVTDFPSQKVLQSDKVIAIPHLGASTE
                     ESEENCAVKAAQELMDYLTYGNISNSVNLPNAKMDMNSPFRITCIHDNKPKMISQITD
                     ILKDANIENLMNKSKKDIAYTIIDLDSKVDITPIEKINGIIKVSTYKK"
     gene            complement(5943..7028)
                     /gene="serC"
                     /locus_tag="ACZPDN_02115"
     CDS             complement(5943..7028)
                     /gene="serC"
                     /locus_tag="ACZPDN_02115"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR1297452.1"
                     /translation="MTRVYNFSAGPACLPEWVLKKAASEMLDAHNSGMSVMEMSHRSS
                     EFKDIMDTCKARLKRLMNIPDEYEILFLQGGASLQFTMIPLNLEKHNVDLINTGQWTK
                     KALKEHEKLGKVNVVASSAEDNFTYIPKIKQEDLSEDSDYVYICENNTIYGTKYKELP
                     PHAGKLLVADLSSCILSERCDVSKYDLIFAGAQKNMGPAGLTVVIIKKDLIGLADEKT
                     PSMLNYKTYADNDSMFNTPPTYAIYICGLVLEWLETQIGGLENMEKINRQKAKLLYDY
                     IDASKLYSNPVVPEDRSYTNVPFVTGDKDLDTKFVQEAKEAGIVNIKGHRTVGGMRAS
                     IYNAMPLEGVEALIAFMRKFEEENYNA"
     gene            7248..8123
                     /locus_tag="ACZPDN_02120"
     CDS             7248..8123
                     /locus_tag="ACZPDN_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HIR1297453.1"
                     /translation="MNKFLKGAMIVGLSAAMVGCSSNAASNDSDSKGKSLSGTITAAG
                     SSALKPLVDVAAEQFSNENPDVSITVDAGGSGEGLKQVADKTVTLGNSDVAAEEKLDK
                     DQAKDLVDHQVCVVTMAPIVNKDVGVKDLTKQQLIDIFTGKTTNWKDVGGPDEDIVLV
                     TRPTSSGTRATFQKYALDGNEEASNASMETDDSGVLLQNVTDTKGAIGYVALSYLVDN
                     DDVDTVAIDGVEPTLENTYSGDYKVWTFEHMYTNGEPDKATKAFLDYIMGEKFGKKME
                     SLGYGVSSKMEKTDH"
     gene            8185..9078
                     /gene="pstC"
                     /locus_tag="ACZPDN_02125"
     CDS             8185..9078
                     /gene="pstC"
                     /locus_tag="ACZPDN_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1297454.1"
                     /translation="MRSKSMFCKEYFWQILVTIGGLFVIFVTLLIGAFLVYKGSDTFL
                     KFHHTLQEFLGSANFAPMDNANSKGHVGALIFIVGSLCVCGLALLIATPFALGSSIFM
                     TEISPKFGEKFYRPMVEIFAGIPSVVYGWVGLTVLIPFLKKIFNLQVGHSILAASLVL
                     SIMIFPSITSVASDAIRAVSKETRMAAYGLGSTRWQTIYRVVIPAAFPGIISGIVLGL
                     ARAFGEALAVAMVIGQTTALPKSIFSTSKTLTTEIAAQMGNAMEGGEMKAALWTMALL
                     LFLISLLFITIIHKFSKPKGE"
     gene            9081..9959
                     /gene="pstA"
                     /locus_tag="ACZPDN_02130"
     CDS             9081..9959
                     /gene="pstA"
                     /locus_tag="ACZPDN_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1297455.1"
                     /translation="MDSRIKRAKMVDQCMTILFYGISLFFFLLLIGFAGKVIIGGFLG
                     AKPEMFGFSRSGSIGNQVFNTIYLVFISLLVSVPIGVLAGIYLAMYAKPGLVTRFLRT
                     CIETLSSLPSIVVGLFGYLIFLVVLGLSKSLLAGALSISILSLPLITTTTEDAIKGLP
                     EGYYQGSMGLGATKWQTIFHVLLPACLSRIMTGVILAAGRGFGEAAVLLYTTGSGSDL
                     RWNNFSFTSPTSPINVLRPAETLSIQIWNLQTNGQDRALANLSSAVLMILVLAFSIGA
                     NVWSRHISKKQAGFEK"
     gene            9971..10723
                     /gene="pstB"
                     /locus_tag="ACZPDN_02135"
     CDS             9971..10723
                     /gene="pstB"
                     /locus_tag="ACZPDN_02135"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR1297456.1"
                     /translation="MNNKFTVNDLNLYYGDFHALKNINMDIKEKEITAFIGPSGCGKS
                     TFLKTLNRMNDLVESAKITGNICLDGTDIYKSMDAIELRRRVGMVFQQPNPFPKSIYD
                     NIAYGPRIFGIKKKSELDEIVERSLRQAAIWDEVKDRLHKSALGMSGGQQQRLCIARA
                     LAMQPEVLLMDEPTSALDPISTSKIEELTLELKKDYTIVIVTHNMQQAMRISDKTAFF
                     LLGDLIEYSDTETLFSTPKEKKTEDYITGRFG"
     gene            10723..11376
                     /gene="phoU"
                     /locus_tag="ACZPDN_02140"
     CDS             10723..11376
                     /gene="phoU"
                     /locus_tag="ACZPDN_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR1297457.1"
                     /translation="MVLPTKFLKQTRQLELTMTQMSQDCLKGLWFCKHAILDGNKTLV
                     QDVSQMHEEVSEQGREAEQICMKILLLQHPVAKDLRRITVATNTIRDLTRIMDQEKEV
                     AELICALNMEDLIVDDCIQQQFSIAKEMITLAIEAFIQKDEKAANQVITMDDQADAQY
                     EMAKEIYVDLLAKKEHDTGTLVDVLLVGKYLERICDHCVNIGKWVLYQKSGIFEEIE"
     gene            complement(11419..11494)
                     /locus_tag="ACZPDN_02145"
     tRNA            complement(11419..11494)
                     /locus_tag="ACZPDN_02145"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11459..11461),aa:Ala,seq:tgc)
     gene            11559..12713
                     /locus_tag="ACZPDN_02150"
     CDS             11559..12713
                     /locus_tag="ACZPDN_02150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR1297458.1"
                     /translation="MKKQWTWIMILCVFNFTLMGFILTHPTSKNQDAKIVEKEEKKSF
                     TFVGVGDNLYHNTQYMCQEQNEYYNFDSYYEMTNKYTQKADLAYINFETLCIGEEYGL
                     SGYPTFNGPTEILSSVNKAGFDWWSLSSNHSMDRGVEGLLKQIELVRENYPDVITTGS
                     HTSKKDRNRTLVKEINGIKVGFLGYTYGLNGFVLPEDKPWLVDLIDKEQMKKDMEALS
                     KVSDIQLVSMHWGDEYQTYPNDEQQDLANYLNELGAEVVIGSHPHVIQPAKIIKGKKQ
                     DTLVYYSLGNYTSTQDVDERMVGGMASFTVNYDPNTKKTSFTDTKFIPLITWFDAGYS
                     TWKTYSIEDYNDSIASTHRLSGEYDMSKEWVQQYVQSVMQDSEGIEVVLE"
     gene            12710..13168
                     /gene="smpB"
                     /locus_tag="ACZPDN_02155"
     CDS             12710..13168
                     /gene="smpB"
                     /locus_tag="ACZPDN_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR1297459.1"
                     /translation="MSQNQKTIAENRKARHDYELYDRFEAGLVLQGTEIKSIRRGKVQ
                     LKDSYISFQKGEAFIKGMHISPYEFGNRFNHDETRDRKLLMHKQEIQKLEQSARIKGY
                     TVVPVRIYLYKGLAKLEIALAKGKNLHDKRESQKAKDAQREIQKALKNQY"
     gene            13190..13580
                     /gene="ssrA"
                     /locus_tag="ACZPDN_02160"
     tmRNA           13190..13580
                     /gene="ssrA"
                     /locus_tag="ACZPDN_02160"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            13672..14046
                     /locus_tag="ACZPDN_02165"
     CDS             13672..14046
                     /locus_tag="ACZPDN_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR1297460.1"
                     /translation="MRIAVSYKDGEVFQHFGHSSEFKIYDTDHNEIIKSEIITVEGHG
                     HVGVSNALKEQNVNIVLCGGIGQGAKTALAGFGIHSFGGVTGNADEAVKKYLRGKLEF
                     TTDVTCTKHESGKSHSCGGHCH"
     misc_binding    14096..14299
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14340..16088
                     /gene="thrS"
                     /locus_tag="ACZPDN_02170"
     CDS             14340..16088
                     /gene="thrS"
                     /locus_tag="ACZPDN_02170"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1297461.1"
                     /translation="MELVNVLENEHLSMLNHSCAHMMAQAVKRLYPNAKFWVGPVISE
                     GFYYDMDLGDVKLKEEDLAKIEKEMKKIAKDGKRIVREEISKEDALEMFKEDPYKIDL
                     INGLEDGNITIYRQGEFADLCRGPHVETVKMCKNFKLLKHSGAYWKGDANNKMLQRVY
                     GICFDTKEELEAHLEALEEAKKRDHKKLGKEMGLFTISEFAPGMPIFLPDGMILRNIL
                     EQYWYQEHTKEGYQFIKTPIMMSKGLWELSGHWDHYKDNMYTSMVDDREFAIKPMNCP
                     GALLVYNSTLHSYKDLPLRLGELGQVHRHEASGALNGLFRVRTFTQDDAHLFVTPDQL
                     EEEVKKVLQLVDRIYSVFGLPYEIELSTRPESGYIGSEEIWDASEKALAQACVHAGKE
                     YKVNPGDGAFYGPKLDIHIKDSLGRVWQCGTVQLDMNLPERFECKYVDADGTKKTPIM
                     LHRVVYGSVERFIGILIEHFGGHFPLWLAPRQFVVIPVHHEKHVEYANKVCDALTALG
                     MRATVDSRNEKLGYRVREAQLQKVTYQIVVGDGEQENNTVTIRQSGKKDSVTLSLDEA
                     LAKFKAEVDNKTLFGK"
     gene            16235..16325
                     /locus_tag="ACZPDN_02175"
     tRNA            16235..16325
                     /locus_tag="ACZPDN_02175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16269..16271,aa:Ser,seq:gct)
     gene            16346..16421
                     /locus_tag="ACZPDN_02180"
     tRNA            16346..16421
                     /locus_tag="ACZPDN_02180"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16379..16381,aa:Asp,seq:gtc)
     gene            16427..16500
                     /locus_tag="ACZPDN_02185"
     tRNA            16427..16500
                     /locus_tag="ACZPDN_02185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16459..16461,aa:Gly,seq:gcc)
     gene            16629..17366
                     /locus_tag="ACZPDN_02190"
     CDS             16629..17366
                     /locus_tag="ACZPDN_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297462.1"
                     /translation="MAVELDKKIGKYMFAGKIYKDGKCIKRYRKRGFDSKWEAQKAEV
                     EFRKDFFMLPSDMNFDRLYKAFKEYNKKYVKESTLKSDEYLYNVLSKEMKDIDFLDKR
                     QMQKLINKFDDKYSKAYVSRIYFFLNKLYKFGVTSEYIPTNPMTFVKRDLRLNERKEE
                     MTIWQQYDFDLFIEEVDEQMMKCFYSVLFYMGLRKGEAMALQWKDIDFRKQTIDINKT
                     YRYKEKDPNKWLTPPKTNNSYKNYHNA"
     gene            17359..17667
                     /locus_tag="ACZPDN_02195"
     CDS             17359..17667
                     /locus_tag="ACZPDN_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR1297463.1"
                     /translation="MPNTLSKMLREWFLECSKWDDFTKDKFVFGYYKPISPQTVQRRF
                     DDAYNKAKEKDDGLPKIRIHDFDIAKRLGDTVETLHNTYAHWFDTKDKSIVDMMNKLL
                     "
     gene            17720..18160
                     /locus_tag="ACZPDN_02200"
     CDS             17720..18160
                     /locus_tag="ACZPDN_02200"
                     /inference="COORDINATES: protein motif:HMM:NF046260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6978 family protein"
                     /protein_id="HIR1297464.1"
                     /translation="MKEEEIRKLLNGFKRLQEKQIEIPNPAERNEYEAYLIEEFKHKF
                     KIILRRGNRQPDKLTYLALDKEARLPLMRLDVVPDNNAHKNPDGEIIYGTHLHVFTEQ
                     YQDKYAIKFDINNPNLVNYCVEFLKRFKVIELNKNCVVEMPRFC"
     gene            18188..18952
                     /locus_tag="ACZPDN_02205"
     CDS             18188..18952
                     /locus_tag="ACZPDN_02205"
                     /inference="COORDINATES: protein motif:HMM:NF020436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HIR1297465.1"
                     /translation="MENLKDFAKNYLNFLKSNLSVKKIETAHEIVLPFEDHIGDSIVC
                     YVDDKKENGMFLVSDDGYIINNLIDTGINIDKKSSRRKTIEQICMLSGVSLSDDNEMT
                     VLSSEKDLPSKVHQLAMTMLQIDDMYLTNTVRTTSYFLEDVTNFFIKNDIYFSDNVSF
                     VGRSGLTQKFDLCFQRNKNHNERLCKAINNPTRDSLTTTVFAWLDIEKTRNDAKCIVI
                     LNDAQTIKSDIVQGFKEYGIMTVPFSNLEKEKGLFS"
     gene            complement(19688..>19960)
                     /locus_tag="ACZPDN_02210"
     CDS             complement(19688..>19960)
                     /locus_tag="ACZPDN_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HIR1297466.1"
                     /translation="QEDGTSQAAPQCQKSEGTEKGGYRGRQKRKSGNGHNQFDEAFFI
                     SSTEIKKRAYYMGADAIIGMKEELNLDTNGFQHFYMQMYGTAVKFK"
BASE COUNT         6839 a         2975 c         3732 g         6404 t
ORIGIN      
        1 gtagaagtag atgaagaggg aacaacagct cttcaaaaat tattcaatga aattggacca
       61 gcatatgcag atcgtaatgg tggatacact cgtgtagtta aaacacgtat ccgtcgtgga
      121 gatgcagctc caatggctat tgtacaattc gttaagtaat tctattaatg aataaaagac
      181 actgattttt gatcagtgtc tattttttta cgataacttt ttgaatttga tcctctttcc
      241 aaatgacaag atcacaatca aatccctctt taacttgtcc agtatgttga aatcctaata
      301 cattcgctac attctttgtg aagaatggca aagcattttc atatccaagt tcatttttaa
      361 gatggatgaa ttcatttctt aaatttgaaa ttggcgtaat accaatttct ttaacggaac
      421 catcttcatt gtatgtagac caagatcctt gagcatcact agaaatggtt actttctctg
      481 tgtttttgat ctttcgaatg taatttaatg ttttgccatt accatcacaa gtgatgtcaa
      541 ttgttccgcc catctctgta aatcttatag cttgttcaaa taaatgagga tttcttgtac
      601 agtgtgttgg ctggaataaa gaaataggaa tatccgcctt ttctaaggcc ttaaagacaa
      661 gctctaaggc ttgtttttca tcacccatat gaattgtcaa atttgcctgt tttccagcta
      721 taagactcgc tgtacgaatt ctagaagcca aacgaattaa ttcatcttct gtaatatgac
      781 tcgatctatg atcacttaac gcaagtttnn nnnnnnnnaa caaacacaat atcatcttca
      841 attgaacttg tcagtgtatt actaggatag gcatagctac ctgttaagat aaacgtatgt
      901 acacctttct gtgtgagttc tttcgctttc gcaaataatt cttttgtact tcttgtataa
      961 ccatccgttc ccaataatcc aatgacagtt gtgatacctg catctaatat ttcctgtaca
     1021 tcaataggtc tagctctaga aacaaatcca gcttcacctc caccaccagt aatatggaca
     1081 tggcgatcaa tcaaacctgg cgttaaaatg catcctgtgc catcaattgt ttcaattgga
     1141 taagaaattt cgatatgatc atcaattttt tcgattttag aatctgaaat caatacatct
     1201 tttactccta aatttttagg tgcataaatt tgaatatttt taataagttt catgcgtgtc
     1261 acctcaaaaa gtattatata ataaaacgca ggtgatgtta tgttaatctc aacagaaagt
     1321 ttaaaaaaga atataaatga caaatgcatt ttaaaagatg catcttttac aatagaagat
     1381 acggataaaa ttgcgttaat tggggtcaat ggtacaggta aaagtacttt attaaagata
     1441 cttgcaggta ttgaaaatta tgattcagga accattataa aaaagaatgg aattaagatt
     1501 cattatttgc cacaaaatcc agaattcaaa acagattgtg tatgggatga aattcaactt
     1561 gtgaattcaa aaaatgagca ccccgtcgca gaatttgaat tgaaatcgat cctaacgaaa
     1621 cttggtatca ctgactttca aagtgtaatg tcaactatgt caggcggaca aagaaaaaga
     1681 gtggctcttg ccttagcgct atgtacaagt tgtgacttgt tgcttctaga tgaaccaacc
     1741 aatcatttgg ataatgatat ggtcgaatgg ctagaaaatt atttaattcg ttcaaaaagt
     1801 gcaatattta tggtcacaca cgatcgttat tttttagatc gtgtttgtac aaaaatgatt
     1861 gaattagatc aaggcgattt atatattcat aatgggaatt atgaggtgta tctagaaaat
     1921 aaggaaacgc gtatagaaca agaaaaactt gctgcccata aacataagaa tttatataaa
     1981 aaagaattag aatgggtaag agcgggtgta caagcccgtg gaaccaaatc caacagtcga
     2041 cttcaaaggt ttgaagaatt gcgtcagaaa cgctttaaac aacaagaaga gtcattaaaa
     2101 atcaatgcgg ctatgcaacg attgggaaat aaaaccattg aatgtatgaa tttagggttt
     2161 gattatccag aaaagcaatt gtttcatgat tttaactatg tattattacg ccacgaccgt
     2221 attggtatta ttggagaaaa tggatgtggt aaatctacat tacttgatgt tattgcaggt
     2281 ttaaaacaac caacttttgg aactattgaa tatggcacaa ctgttaaaat tggatatttt
     2341 aggcaagccg atcaaggcgt agatttaaat atgcgtgtta ttgattatat tgaagaagtt
     2401 tcaaaagtta ttgaatacga tcgaatgact ctaagtgcat cgcaaatgct tgaaagattc
     2461 ttatttccaa gaaacatgca ctatactaca ctatcaagat tatctggtgg agaaagaaga
     2521 agactttatc tattaagagt tttgatgcaa gaaccaaata ttttattatt ggatgagcca
     2581 accaatgatt tggatatctt aacattagac gtattagaag actacctaga taattttcaa
     2641 ggtgccatta ttactgttag ccacgatcgt tattttttgg atcgagttgt agataaagtg
     2701 tttgttcatc aagaagatgg aacatttaaa caatatagtg gtggatatag tgattattta
     2761 gtaaaatcaa aagatgagaa taaaaaggtg gttatacaaa aaccaattga aaagaaaaag
     2821 cgttctttat tatcttacat ggagaaaaaa gaattagaac aattaccaga gaaaatggaa
     2881 ttgttggaaa ctgaaattga agatttagac catcaaatga atgaatgtca aagtgatttt
     2941 gcgaaattaa aagaattaag tgatttaaga acgcaaaaag aagaagaatt agaaattttg
     3001 acaaatcgct ggatggaatt agaagaaaaa aaggaaggat aaagttttaa ttggatatgg
     3061 agaatagggt attttttaaa aaaccatccc atgtgttttt gttagctatg ctatgttgta
     3121 tactatgggg aagtgcggct ccctttatta aatggggata tcaattattt aatattgaag
     3181 gcgtaccaag catattgatg tttgcgggta ttcgctttgc gttagccgga cttttagtca
     3241 tattatttgg ctctttctta cagaaaaaag tattagtcgc aaaaaaagaa aacattcaag
     3301 gcatatgtac attagctcta tttcaaacgg ctggacaata ctttttctac tatataggac
     3361 tttcacatac atcgggcgta aatggttcaa ttattaccgg taccagtgcc tttatatctt
     3421 tattagtagc cagtttgctc tttcattatg aaaagctgac actaaggaaa acatggggat
     3481 gttttatcgg attcttaggt attttattta tgaatatgag tggtgctagt gcaaactttt
     3541 catttatagg cgaaggcttt gtgttcatgt cacaattatg tggagctata tcagccgctt
     3601 ttattaagaa gtttttttaa aaacaaaatg ccgtactatt aagtggatac caattctttt
     3661 taggtggtgt tttactaatt atcattggat atttgatggg aggacatatt acatttgaat
     3721 tcaatctagg ccttctagta ttgattcatc tagcccttgt atccgctatc gcctatacga
     3781 tttggggact tttattatct gtaaatcctg ttagtaagat tggtgtctat atgtgtgtaa
     3841 caccaatcat tggtgttatt ttatcaagtg ttgtcttaca tgaatccaat caggcattcc
     3901 aattttcaag tttaattgcc ttgattctag ttagcttaag tgtattttta gtcaataaag
     3961 aaacgcagac agattaagtt ctgtcttttt ctttgcctaa aaaaagatcc taggcttatc
     4021 taggatctaa taattcaaag caacgcccta catcacttgg ataatgagca tcacttgcag
     4081 taacaatttg tacattattt tcttttagga tatttctaaa atcagcattt aagccaatat
     4141 caggatgatg atatgaataa tgagctttag tgttgtcttc cattttaatg ttgtattcat
     4201 ttgccagacg agcgatatga tggtaggtag gatgtaaatc gtagcctgga tcaatcgaat
     4261 acaatttaat cacatctgga tgacccactt gatcaaagag attggattgg attagttttt
     4321 caatagagtc ataataggat ttataaatag tagaaggatc atttttgtcc caaagcaatt
     4381 ctttagaaaa ggcatccata tcatataaat agccatttac agaatgtaca ctacctatta
     4441 gaaagtcaaa ttgataatgt tcaaggattg aagataatag actttctgtt tcaggcgtat
     4501 aacatacttc aagaccaaaa cgcacatcaa tgtcaaaagt ttcttttttt aaggctgcaa
     4561 ttaattttaa ataatcatct atggagtttt tttgtttctt taaaaaccac tcttcttgta
     4621 atttagatac ttcacaaact cttttataac agtctttaaa ttcaaagaat ctatgtgtgt
     4681 gatctaaaat ctgaagatga gtaattcctt tttctttggc agcatcaata aacttacaaa
     4741 catattcaat gcttaaatca ccattttcta aatgtgtatg tgcatcaatc atagtaccat
     4801 cctatttctt ataagtagat actttaatta taccattgat tttctcaatt ggtgtaatat
     4861 cgacttttga atccaaatca ataattgtat acgcaatatc ttttttagat ttattcatca
     4921 aattttcaat gttggcatcc tttaaaatat ccgtaatttg agaaatcatt tttggtttat
     4981 tatcatgaat acaagtgatt ctaaatggtg aattcatatc cattttcgca tttggtaaat
     5041 tgacagaatt tgaaatattt ccataagtca aataatccat caattcttga gctgccttta
     5101 cagcacaatt ttcttcactt tcctcggttg aagcacctaa gtgaggaatc gcaatgactt
     5161 tatcgctttg aagtactttt tgactaggaa aatcagttac ataagatgca actttatttt
     5221 catttaacgc ctctaataga gcatcttcat caacaaggcc gccacgcgca aagtttaata
     5281 atttgacgcc atctttcatc ttgtcaaaac tatctttatt gataaagctt tttgttccat
     5341 catttaaagg tacatgaatc gtaataaaat cacattgacg atatagatca tcaatattgg
     5401 tgacatgatt tacatatcga gataaattcc atgcagcatc aacagacata taaggatcat
     5461 atccatatac tttcatcccg aaacgtaaag ctagattggc aacttgaata ccaatagcac
     5521 cacaaccaat gacacctaaa gatttaccag ttaattcatg accagcatat tttttctttt
     5581 gtttttctac atcctttgcg atgttctcat tttctttttg cgtttggatc cattcaatac
     5641 catgataaat atcacgactt gccagtaaca tacccgcaaa cacaagttct tttacggcat
     5701 tgctatttgc accgggagta ttaaatacgg caatcccttt tttagtacat gtatcaatat
     5761 caatgttgtt gacaccagca cctgcacgag cgatggcttt aaggttggca ttatattcaa
     5821 tatcatgaag attggcacta cgaactaaaa tagcatcttc atcttccatg ttttcgttta
     5881 cttcataatc tggcgtaaac tgatctaaac caatttgcgc aatattattg tagagtttta
     5941 ttttaggcat tataattctc ctcttcaaat tttctcataa atgcaattag ggcttctaca
     6001 ccttcaagtg gcatggcatt ataaatactt gcacgcatac caccaaccgt tctgtgacct
     6061 ttaatattca caattcctgc ttctttagct tcttgaacaa atttagtatc taaatccttg
     6121 tcacccgtca caaaaggaac atttgtataa gaacgatctt caggtacaac aggatttgaa
     6181 tagagtttag aagcatcgat atagtcatat aataatttag ctttttgacg attgatcttt
     6241 tccatgtttt ccaatccacc aatttgtgtt tctagccatt caagtaccaa accacaaata
     6301 tagatcgcat aagtaggtgg tgtgttaaac atagaatcat tgtcagcata tgttttgtag
     6361 ttcaacatgc tcggtgtttt ttcgtcggct aaaccaatca agtctttttt tataataacc
     6421 acggtcaatc cggcaggacc catatttttt tgggcaccgg caaaaattaa atcgtattta
     6481 gaaacatcgc atctttcact taaaatacaa cttgataaat cagcaactaa aagcttacct
     6541 gcatgaggtg gaagctcttt atatttagtt ccatagattg tattgttttc acaaatatat
     6601 acataatctg aatcctcact taaatcttcc tgcttaattt taggaatata tgtaaagttg
     6661 tcctctgcac tagaagcaac tacatttact tttcctaatt tttcatgttc ttttaaagct
     6721 ttctttgtcc attggcccgt attgatcaaa tctacgttat gtttttctaa attcaaagga
     6781 atcatagtaa attgtaaaga agcacctcct tgtaagaaca aaatttcata ttcatctgga
     6841 atattcatca atctttttag tcttgcttta caagtatcca taatatcctt aaattcactg
     6901 gaacggtggg acatctccat aacgctcatt ccactattat gagcgtccaa catttcgctt
     6961 gcagcttttt ttaataccca ttctggtaag catgcgggac cggcactaaa gttatatacg
     7021 cgtgtcatat tgttacctcg gtgaaaatat ttccaccttt cctttcttta tgtttcatga
     7081 ttatacattt atttacattt aatttcaaga aatttgttca taaaataaat tcaagtgtat
     7141 ttaaaatcaa aacaaatcat ttaacgaaag tttaacagaa ccattctttt tgtttaacaa
     7201 acaagcacta tgatatgtgt gtcgaaagaa aagaggacaa agaaaatatg aataagtttt
     7261 taaaaggtgc aatgatcgta ggattatctg cagctatggt aggatgtagt tcaaatgctg
     7321 catcaaatga cagtgattca aaaggaaaaa gtttaagtgg tacaattaca gccgcaggtt
     7381 caagtgcatt gaaaccattg gtggatgttg cagctgaaca attctctaat gaaaaccctg
     7441 atgtatctat tacagtagat gctggtggat ctggagaagg cttaaaacag gtggctgata
     7501 aaacagttac acttggaaac tccgatgttg cagctgaaga aaaattagat aaggatcaag
     7561 ctaaagattt agtcgatcac caagtatgtg ttgtaacaat ggctccaatc gtaaataaag
     7621 atgtaggtgt taaagattta acaaaacaac aattaattga cattttcaca ggaaaaacaa
     7681 caaactggaa agatgttggt ggaccggatg aagatattgt attggtgact cgcccaactt
     7741 catcaggaac aagagccaca ttccaaaaat atgcattaga tggaaatgaa gaagcaagca
     7801 atgcctcaat ggaaacggat gattcaggtg tattgttaca aaatgtaaca gatacaaaag
     7861 gtgcaattgg ttatgtagca ttatcttatt tagtagacaa tgatgatgta gatactgtcg
     7921 caattgatgg ggttgaacct acattagaaa atacttatag tggtgactat aaagtttgga
     7981 catttgaaca catgtataca aatggagaac cagacaaagc aacaaaggcg ttcttagatt
     8041 acatcatggg tgagaaattc ggtaagaaaa tggaatcatt aggctatggt gtttctagta
     8101 aaatggaaaa aacagatcac tagtaaagtt ctttaacgga cattgaaata tatgtccgtt
     8161 attcatttag tttggaggaa tttgatgcgt agtaaaagca tgttttgtaa agaatatttc
     8221 tggcagattc ttgtaacaat tggcggtctg tttgtgatat ttgtaactct attaattgga
     8281 gcctttctag tttataaagg ctcagataca tttttaaaat ttcatcatac tctacaagaa
     8341 tttcttggta gtgccaactt tgcacctatg gataatgcca attcaaaagg acatgtagga
     8401 gccttgatct tcattgttgg atcactatgt gtttgtggat tggcactatt gattgccaca
     8461 ccatttgcgt taggatcttc tatttttatg acagaaattt cacctaagtt tggtgagaag
     8521 ttctatcgtc ctatggtaga aatctttgcc ggaattccta gtgttgttta tggatgggtt
     8581 ggattaacgg ttttgattcc atttttaaag aaaatattta atttgcaggt aggacactcc
     8641 atacttgcgg caagtttagt tctatcaatt atgatttttc catcgattac aagtgttgct
     8701 agtgatgcaa ttcgagccgt ttcaaaagaa acacgaatgg cggcttatgg acttggctca
     8761 acacgttggc aaactatata tcgtgttgtg attcctgctg catttccagg cattatttca
     8821 ggtattgttt taggactggc aagagcgttt ggagaagctt tagccgttgc gatggtcatt
     8881 ggtcaaacaa cagctttgcc taagagtatt ttttctactt caaaaaccct aacaacggaa
     8941 attgcagctc aaatgggaaa tgcgatggaa ggcggagaaa tgaaggcagc cctatggaca
     9001 atggctttat tattattctt gatttcgtta ttgtttatca caattattca taagttttct
     9061 aaaccgaaag gagaataaag atggattcta gaattaaaag agcaaaaatg gtagatcaat
     9121 gtatgacaat tctattttat ggtatctcct tatttttctt tctactcctt ataggttttg
     9181 ccggtaaagt gattattggt ggctttttag gggcaaaacc agaaatgttt ggcttttctc
     9241 gatctggcag tattggaaac caagtcttta atacaattta cttagtattt atttcattgc
     9301 ttgtatctgt tcctattggt gtgttagctg gcatatattt ggctatgtac gctaaaccag
     9361 gtcttgtgac acgtttttta aggacttgta ttgaaacatt atcttcttta ccaagtattg
     9421 tagtgggttt atttggatat ttgattttct tggttgtatt aggattatca aaaagtttat
     9481 tagcaggcgc tttatctatt tcgattctaa gtttaccttt gattactacc accacagaag
     9541 atgcgataaa aggtttacca gaaggctatt atcaaggaag tatgggactt ggagcaacaa
     9601 agtggcaaac tatatttcat gtgctattgc ctgcgtgtct atcacgtatc atgaccggtg
     9661 ttattttagc ggctggtcgt ggatttggtg aggctgccgt attactttat acgactggtt
     9721 caggaagtga tttgcgttgg aataacttta gtttcacttc tccaacatca ccaatcaatg
     9781 tgttaagacc ggctgaaaca ttgtccattc aaatttggaa cttacagaca aatggtcaag
     9841 atcgtgcatt ggcgaatcta tcgagtgctg tattaatgat tttagtatta gcgtttagta
     9901 ttggtgcgaa tgtctggtca agacatatct ctaaaaaaca agctggattc gagaaataga
     9961 aaggttaact atgaataata aatttacagt gaatgatttg aatttatatt atggggattt
    10021 tcatgcctta aaaaatataa atatggacat caaagaaaaa gaaattacgg cattcattgg
    10081 tcctagtgga tgtggtaaat cgacattttt aaaaacgttg aatcgtatga atgacttggt
    10141 ggaaagtgcc aaaattacag gcaatatctg tctagatggt acggatatct acaaaagtat
    10201 ggatgcgatt gaattaagac gtagagttgg aatggtgttt caacagccta atccattccc
    10261 aaaaagtatt tatgacaata ttgcgtatgg tccaagaatt tttggaatca aaaagaaaag
    10321 tgaattggat gaaattgttg aaagaagctt aagacaagct gccatttggg atgaagtaaa
    10381 agatcgccta cataaatcgg ctttaggtat gtcaggtggt caacaacaac gtttgtgtat
    10441 cgcaagagcc ttggctatgc agccggaagt tttattgatg gatgaaccta caagtgcttt
    10501 agatccaatt tcaactagta agattgaaga attgacttta gaattgaaaa aagattatac
    10561 aattgttatt gttacccaca atatgcagca agctatgcgt attagtgata aaacagcctt
    10621 cttcttattg ggggatttaa ttgagtatag tgataccgaa acattgttct ctacgccaaa
    10681 agaaaagaaa acagaagact atattacagg gaggtttgga taatggtttt acctacaaag
    10741 tttttaaaac aaacaagaca gttagaactg actatgacac aaatgtctca agactgttta
    10801 aagggcttat ggttttgcaa gcacgctatt ttggatggaa acaaaacttt agttcaagat
    10861 gtttcacaaa tgcatgaaga agtgagtgaa caaggaagag aggctgaaca aatttgcatg
    10921 aagatccttc ttcttcagca tccggttgcg aaagatttaa ggcgtattac ggtagctaca
    10981 aatacaattc gtgatctaac acgcatcatg gatcaggaaa aagaagtcgc agaactaatt
    11041 tgtgcattga atatggaaga tttaattgtg gatgattgta ttcaacaaca gttttcaatt
    11101 gcgaaagaaa tgattactct tgcgattgag gcttttattc aaaaggatga aaaggcagca
    11161 aatcaagtga ttacaatgga tgatcaagcg gatgcacaat atgaaatggc gaaagaaatc
    11221 tatgtggact tattggctaa aaaagaacat gatacaggta cattggtaga tgtcttatta
    11281 gttggtaaat atttagagag aatttgtgat cattgtgtaa acattggaaa atgggttctt
    11341 tatcaaaagt ctggtatttt tgaagaaatt gaataaaaaa aagctgtaag caaatttgct
    11401 tacagcgtaa tttttacttg gtggagcgta tcgggatcga accgatgacc ccctgcgtgc
    11461 aaggcaggtg ctctcccagc tgagctaaca ccccaagtgc cttataatat tatcgaattt
    11521 ttttggaaaa agcaagtatt ttttgataaa attaatatat gaaaaaacag tggacatgga
    11581 tcatgattct atgtgtattc aattttactt tgatgggatt tatcttaacg catccaacat
    11641 caaaaaacca agatgcaaaa attgtggaaa aagaagagaa gaagtctttt acatttgttg
    11701 gtgtcggaga taacttatat cataatacac aatatatgtg tcaagaacaa aatgaatact
    11761 ataattttga ttcttattat gaaatgacga ataaatatac gcaaaaggcc gatttggctt
    11821 atatcaattt tgaaacattg tgtataggtg aagaatatgg attgagtggc tatcctactt
    11881 ttaatgggcc tacggaaatt ttatcttccg taaataaggc tggctttgat tggtggagtt
    11941 taagttcaaa ccattcaatg gatagaggcg ttgagggctt gctgaaacag atcgaattgg
    12001 ttcgtgaaaa ttatccggat gtcattacta cgggttctca tacatctaaa aaagatagaa
    12061 atcgtacttt agttaaagaa atcaatggta ttaaagtagg ctttttaggc tatacttatg
    12121 gtttgaatgg ttttgtcttg ccagaagata agccttggct tgtggatttg attgataagg
    12181 aacaaatgaa aaaggatatg gaagctttaa gtaaagtaag cgatattcaa ttggtttcca
    12241 tgcattgggg cgatgaatat caaacatatc caaatgatga gcagcaagat ttggcaaatt
    12301 atttaaatga gttaggtgct gaagttgtga ttggatcgca tccgcatgtc attcaaccag
    12361 ctaaaataat caaagggaaa aaacaagata cacttgtata ttattcgttg ggaaattata
    12421 cgagtacaca agatgtggat gaaagaatgg ttgggggaat ggctagcttt actgtaaact
    12481 atgatccaaa tactaaaaag acatctttta cagatacaaa gtttattccg ttgattacat
    12541 ggtttgatgc aggatatagt acatggaaaa catattcgat tgaagattat aatgattcca
    12601 ttgcttcaac acatagatta tctggtgaat atgatatgtc taaagaatgg gttcaacaat
    12661 atgttcaatc tgttatgcaa gattcagaag ggattgaggt ggtattagag tgagtcaaaa
    12721 tcaaaaaaca atagccgaaa atcgtaaggc aagacatgat tatgaattgt atgatcgttt
    12781 tgaagctgga ctcgtacttc agggtacaga aatcaaaagt attcgaagag gaaaagtcca
    12841 attaaaagat agttatatta gttttcaaaa gggtgaagct tttattaaag gaatgcatat
    12901 ctctccttat gaatttggaa atcgattcaa tcatgatgaa acaagagatc gtaagctttt
    12961 aatgcataaa caagagattc aaaaattaga acaatcagct cgtataaaag gatatacagt
    13021 tgtaccagta agaatttatt tatataaagg actagctaaa cttgaaatag ctttggcaaa
    13081 aggtaaaaat cttcatgata agcgtgaatc tcaaaaggca aaggatgcgc aaagagagat
    13141 tcaaaaggcc ttgaaaaatc agtattaata gattatactg atattccttg gggatgttca
    13201 ggcattcgat gagtaaatgt ttggattcgg ccacagccac gaaggatacg tgtaataacc
    13261 aaattaaatt aattgctaaa aataaacttg ctaatgtatt tggtatgaac caaagcatgg
    13321 ctttcgctgc ttaattttat tgcctacgtt tggctttgca gcccctttct gaaatttcgc
    13381 tttcttattt cagactgagt gttaaagaaa agaagataga aataagcatt ggctagctgc
    13441 ttattttgaa tcaactatag ctaaaccaaa agaatttttg tttccatccg aaacttttgg
    13501 ataactagat ggaaaaagct gtaaacgctg tctcgtggga ctttgcttag acaggagttc
    13561 gattctcctc atctccacca aagacaatca acgcccttat ttaaagggcg ttttaattta
    13621 tcgtgatgta cttttgatgt ataattgtta cgaaagaagg taaatttaca tatgagaatt
    13681 gcagtgagct ataaagatgg agaagtgttc caacattttg ggcatagttc agaatttaaa
    13741 atctatgaca cagatcataa tgaaattatt aagagtgaaa taattacagt cgaagggcat
    13801 ggacatgttg gtgtttcaaa tgcattgaaa gaacaaaatg taaacattgt actttgtggt
    13861 ggcattggac aaggggctaa aactgcattg gctggttttg ggatccattc ttttggtggg
    13921 gtaaccggaa atgcagatga ggctgttaag aaatatttaa gaggaaagct tgaatttaca
    13981 acggatgtga cttgtacaaa acatgaatct ggcaaaagtc attcttgtgg tggacattgc
    14041 cattaattct tgagtttttc ttttgacacg ccttgtattt cggtctataa tctaggcaac
    14101 aatagtgata aggatatgaa tacattctat tcaaatgtag agagagtgtg attggtgaaa
    14161 acactttgcg aatgtatgta tttactccct ttgaattgcc cttgaaaaag tgtgccgtaa
    14221 ttcttgcgtt aaaaggtttg aggtgtatac agtaattgta taaacaaagg tggtaccgcg
    14281 tgcgacagac gtcctttgat ggctgtcgct ttttatatgg aaaaatggag gaaaataaaa
    14341 tggaattagt gaatgtatta gaaaatgaac atttatctat gttgaaccac tcatgtgctc
    14401 atatgatggc tcaagctgtt aagcgtttat atccaaatgc taaattttgg gtaggacctg
    14461 ttatttctga aggtttctac tacgatatgg atttaggaga tgtaaaatta aaagaagaag
    14521 atttggcaaa aatcgaaaaa gaaatgaaga aaattgccaa agacggaaag agaattgttc
    14581 gtgaagaaat ctctaaagaa gatgctttag aaatgtttaa ggaggatcct tataaaattg
    14641 atttgatcaa tggattagaa gatggaaata tcacaatcta tcgtcaaggt gaatttgcgg
    14701 atctatgtcg tggacctcac gttgagactg ttaaaatgtg taaaaacttt aaattgttga
    14761 aacattctgg tgcttactgg aaaggtgatg caaacaataa aatgcttcaa cgtgtttatg
    14821 gtatttgttt cgatacaaaa gaagaattgg aagctcactt ggaagcttta gaagaagcta
    14881 aaaaacgtga ccacaagaaa ttaggaaaag aaatgggttt attcacaatt agtgaatttg
    14941 caccaggtat gcctatcttc ttgccggatg gaatgatttt acgtaacatc ttggaacaat
    15001 actggtatca agaacacaca aaggaaggat accaattcat taagactcca attatgatgt
    15061 caaaaggatt atgggagtta agtggacact gggatcacta taaagacaat atgtacactt
    15121 caatggttga tgatcgtgaa ttcgcaatca aaccaatgaa ctgtccaggt gcattattag
    15181 tatataactc aactttacac tcatataaag acttaccatt gcgtttagga gaactaggac
    15241 aagtacatcg tcacgaagca agtggtgcat tgaatggatt attccgtgta cgtacattta
    15301 cacaagatga tgcgcactta tttgtaactc cagatcaatt ggaagaagaa gttaaaaaag
    15361 tattacaatt ggtagatcgt atttattctg tatttggttt accttatgag attgaattat
    15421 caactcgtcc agaaagtgga tatattggtt ctgaagaaat ttgggatgct tcagaaaaag
    15481 cgttggcaca agcttgcgtt catgcaggta aagaatataa agtaaatcct ggagatggtg
    15541 cattctatgg acctaaattg gatatccata ttaaagatag cttaggacgt gtatggcaat
    15601 gtggtactgt acaattggat atgaacttgc cagaaagatt tgaatgtaaa tatgtagatg
    15661 cagatggaac taagaaaacg ccaattatgt tacaccgtgt agtttatgga tcagttgaac
    15721 gtttcattgg tatcttgatc gaacactttg gtggacactt cccattgtgg ttagctccac
    15781 gtcaatttgt ggtaattcca gttcaccatg aaaaacatgt cgaatacgca aataaagtat
    15841 gcgatgcatt aacagcatta ggaatgcgtg caacagtaga ttctcgtaat gaaaaattgg
    15901 gttaccgtgt tcgtgaggca cagctacaaa aggttactta ccaaatcgta gtgggtgatg
    15961 gagaacaaga aaacaataca gttacaattc gtcagagtgg taaaaaggat agtgttacat
    16021 tatcattaga tgaagcttta gctaaattta aagcggaagt agacaacaaa acattgtttg
    16081 gaaagtaaaa gtttagaggt caaaaattgg cctctaattt tttttgtctt tttttaaaaa
    16141 attgcttgca ataagttttt atccatgtta ttattaacgg gcgttacgga taacgcaggt
    16201 tgttctaaaa aataagtatt ctgaaaatat tcttggagaa gtactcaagt ggctgaagag
    16261 gtgcccctgc taagggtata ggtcgggaaa ctggcgcgag agttcaaatc tctccttctc
    16321 cgccatctta tttttggaaa attatggtcc agtggtgtag tggttaacat gcctccctgt
    16381 cacggaggag atcgtgggtt cgagtcccat ctggaccgcc attcatgcag atgtagttca
    16441 atggtagaac acagccttgc caaggctgat acgggggttc aattcccctc atctgctcca
    16501 ttgaaattta agcctttatt taaaggcttt tttatttgtc ttggggtata ttggggtata
    16561 atttgatatt aaaatattga attatacccc tttttttcat attatggaca tataagaggg
    16621 cacaaaaaat ggcagtggaa ttagataaga agataggaaa atatatgttt gccggaaaaa
    16681 tatataagga tggtaaatgt ataaagagat atcgtaagcg tggttttgat tctaaatggg
    16741 aagcacaaaa agctgaggtt gaattcagaa aagatttctt tatgcttcca tcagacatga
    16801 attttgatag actatataaa gcttttaaag aatataataa aaagtacgta aaagaatcaa
    16861 ctttaaaatc agatgaatat ttgtacaatg ttctttctaa ggaaatgaaa gatattgatt
    16921 ttctagataa aaggcaaatg caaaaattga tcaacaaatt tgatgacaaa tattcaaagg
    16981 catatgtatc aagaatatat ttctttttaa ataagctata taaatttggt gttacttctg
    17041 aatacattcc aaccaatcca atgacatttg taaaacgaga tctcagattg aatgaaagaa
    17101 aagaagaaat gacaatatgg cagcaatatg attttgattt gtttattgaa gaagtagatg
    17161 aacaaatgat gaaatgcttt tattctgttt tattctatat gggattacga aaaggtgaag
    17221 caatggccct acaatggaag gacattgatt ttaggaagca aactatagat atcaacaaaa
    17281 catatagata caaagaaaaa gaccctaata aatggcttac accgccaaaa acaaacaata
    17341 gctataagaa ctatcacaat gcctaataca ttgtctaaaa tgcttcgaga atggtttcta
    17401 gaatgttcta aatgggatga tttcacaaaa gataaatttg tgtttggata ttataaacca
    17461 atatcacctc agacagtaca aagaagattt gatgatgcat ataataaggc aaaagaaaaa
    17521 gatgatggat tgcctaaaat aagaattcat gattttgaca tagccaaacg cttaggagat
    17581 acagttgaaa cattgcacaa tacatatgca cactggtttg atacaaaaga caagagcatt
    17641 gtagatatga tgaataagtt gttataaata tgacatgaat aatgtcaata taaaaattaa
    17701 tatagaggtg cacgaggaaa tgaaagaaga agaaatcaga aaattattaa atggatttaa
    17761 gagattacag gaaaaacaaa ttgaaattcc aaatccagct gaaagaaatg aatatgaagc
    17821 ttatcttata gaagaattta aacacaaatt caaaattatt ttgcgtagag gtaacagaca
    17881 acctgataaa ctaacttatc ttgccttaga taaagaagca agattgcctt taatgcgatt
    17941 ggatgtagtt cctgataaca acgctcataa aaatccggat ggtgaaatta tttatggaac
    18001 tcatctacat gtatttaccg aacaatacca agataaatat gcaataaagt ttgatatcaa
    18061 taatcctaac ttggtaaact attgtgtaga gtttttaaaa agatttaaag tgattgaatt
    18121 aaacaaaaat tgcgtggtgg aaatgcctag attttgttaa aatgtttttg tagggaggaa
    18181 tgatgttatg gaaaatttaa aagactttgc aaagaattat ttgaattttt taaaatctaa
    18241 tttatctgta aaaaaaatag aaactgcaca tgaaatagtc cttccttttg aagatcacat
    18301 aggagattca atagtttgct atgtggatga taaaaaagaa aacggtatgt ttttggtttc
    18361 cgatgatgga tatattataa ataatcttat tgatacaggc attaatattg ataagaaatc
    18421 cagcagaaga aaaaccattg aacaaatttg catgttgtca ggggtttcct taagtgatga
    18481 taatgaaatg actgttttat catctgagaa ggatttaccg tctaaagtgc atcaattagc
    18541 aatgacaatg cttcaaattg acgatatgta tttaacaaat acagttcgta ctacatcata
    18601 tttcttggaa gatgtaacaa acttttttat taaaaatgat atttattttt cggataacgt
    18661 ttcatttgtt ggaaggtcag gactaactca aaaatttgat ctatgctttc aaagaaataa
    18721 aaatcacaac gagcgcttat gcaaggcaat caacaatcct actagagata gtctgactac
    18781 tacagtattt gcgtggttgg atatagaaaa gactagaaac gatgcgaaat gcattgtaat
    18841 tctaaacgat gctcaaacaa taaaaagcga tatcgtacaa ggttttaaag aatatggaat
    18901 aatgacagtt ccattcagta atcttgaaaa agagaaaggc ttatttagct aatacaaaaa
    18961 gaaaaacaga tagtgtatgc tgctcaacaa cactatctgt ttttcattct ttgctcattc
    19021 gtttttttct ctgctaatct ttttttgagg aaatagaaaa aagcctaaaa tatttatcat
    19081 gtacacatga ataataacat cacattttaa aaagagtaat gaaaaatgca aaatattaat
    19141 aaatatttac aaaacaaaat tagttaataa tataaccaaa agttcattaa aaattctacc
    19201 cccgaaaaaa attcaatact ggcaatgtga tatataacca tttgcacttt ttagtattat
    19261 atttgtacaa tttaatacaa aacaggcatt gacatataaa atttaagact tattatatat
    19321 tataataagg aaaacgtttt tctttttatt catagaaaat gaatggaaag ggatgatctt
    19381 atggaaaggg atttatggat aaaaaaatta attatcctag cagaaaaaat taacgtggat
    19441 gatctgcaaa ttctgtataa tcatgcacaa agactgctga tatcatctaa aaatgaataa
    19501 cactaatacc tataggtatt tacgcgcaaa tttaaaagaa ggatactaaa tggaacaaca
    19561 aaagagaatt tcaattgcag aacgatttga aggctatgaa ggcccaacac agcaagaaga
    19621 aatttggtct gatgaagtag ttggcaaaga agaaatataa gctagggaat ttaccctagc
    19681 tttattatta tttaaattta acggcagttc cgtacatttg catataaaaa tgttgaaatc
    19741 cgttagtatc tagatttagt tcttctttca tgccaataat agcatctgca cccatataat
    19801 atgcacgctt ttttatttcg gtagaagaaa taaaaaaggc ttcatcaaat tggttatggc
    19861 cattaccgga cttccttttc tgcctgcctc gatatccacc tttttctgtt ccttcggatt
    19921 tctgacactg gggagccgcc tgggacgttc cgtcctcctg
//
LOCUS       DBMDWL010000018        18829 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1004_length_18829_cov_3.258223, whole genome shotgun sequence.
ACCESSION   DBMDWL010000018 DBMDWL010000000
VERSION     DBMDWL010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 18829)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18829)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18829
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1004_length_18829_cov_3.258223"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(102..479)
                     /locus_tag="ACZPDN_02215"
     CDS             complement(102..479)
                     /locus_tag="ACZPDN_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297467.1"
                     /translation="MEQKYYHGNAISKYGLENGRVDYATLAKCFDAVLNNDIMQLTYD
                     IGSWEQVSGTIENTDEIEELEREQDDQEVFQWYIVDDWGARLLKDINQIVCYNETLNM
                     YLWGVTHYGTSWDYVLTSIKIDW"
     gene            complement(1807..2010)
                     /locus_tag="ACZPDN_02220"
     CDS             complement(1807..2010)
                     /locus_tag="ACZPDN_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297468.1"
                     /translation="MAKTSEAKMRANRKYEKKHIRQILLKFHKTHEAEIIEKLDSVPS
                     KNNYVRQLILQDLEREKKEANNK"
     gene            complement(2023..2226)
                     /locus_tag="ACZPDN_02225"
     CDS             complement(2023..2226)
                     /locus_tag="ACZPDN_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297469.1"
                     /translation="MTNLKQLQNVCKEVNEKMDVISEEELKEIVGRYYKDDVISCRQW
                     DFLIGYIERKEKISDSFAFMYSE"
     gene            complement(2458..2751)
                     /locus_tag="ACZPDN_02230"
     CDS             complement(2458..2751)
                     /locus_tag="ACZPDN_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297470.1"
                     /translation="MPRRDTEYEHFKETCGGWFNYHGNIGLRAGDIAMATLFDETELV
                     QIVLTKPYTYNRWWCKIVGFNSDGIEYLVDRTMILQILIDKEYNLRRKRRKTS"
     gene            complement(2762..3094)
                     /locus_tag="ACZPDN_02235"
     CDS             complement(2762..3094)
                     /locus_tag="ACZPDN_02235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1297471.1"
                     /translation="MEILNNNIYWCDLPRYSNTVLCKRRPCIVISNDIQNKGSKTVNV
                     IPITSNLKRTDLPCHVMIDTGHEYGMAKAEQILTINKENVKWHIKPLDRREEKEVKCA
                     LLTQMGII"
     gene            complement(3075..3299)
                     /locus_tag="ACZPDN_02240"
     CDS             complement(3075..3299)
                     /locus_tag="ACZPDN_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297472.1"
                     /translation="MKKMIATTQVETSFKNIEKTLKDNGLYEAYDDMELIKQALIERD
                     RKIYGLQQHNRNLEDKLGRIGGYHYGNPKQ"
     gene            complement(3256..3432)
                     /locus_tag="ACZPDN_02245"
     CDS             complement(3256..3432)
                     /locus_tag="ACZPDN_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297473.1"
                     /translation="MVIHVHPIWAICILFMHRIGTRVVRVPIKEDDDDAVDDVYGMDW
                     NEEDDSNNSSRDEF"
     gene            complement(3486..3695)
                     /locus_tag="ACZPDN_02250"
     CDS             complement(3486..3695)
                     /locus_tag="ACZPDN_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297474.1"
                     /translation="MMGLKKRNKPKESTDVLIKLKISVPDVNNSDSCNVVDSLLNEIW
                     DAAWSKEGVEVENLKATYMKEKIAK"
     gene            complement(3670..3873)
                     /locus_tag="ACZPDN_02255"
     CDS             complement(3670..3873)
                     /locus_tag="ACZPDN_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297475.1"
                     /translation="MIYFVAGFFIGGIASMLLYSLIVSERINELELENCRLIDDLNKA
                     EYEVRKYKYQHRRYGYDGFEETK"
     gene            complement(3870..4388)
                     /locus_tag="ACZPDN_02260"
     CDS             complement(3870..4388)
                     /locus_tag="ACZPDN_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297476.1"
                     /translation="MKTKQEYIDALNRIEQVYYNFDGCMSSMNKFKEDINLLAGLVNE
                     HFDEKQDEKKETNLDHYKHEILEACTWDLAVVKGIPRQCNKTNCSDCDFKNILRGCHR
                     KVREWLEQPYKKTAIKLTKFEVDLLQINSIKDFRFKNINVLTLMKEKGHFKDVDKDAT
                     IEDILANCEVEG"
     gene            complement(4388..4591)
                     /locus_tag="ACZPDN_02265"
     CDS             complement(4388..4591)
                     /locus_tag="ACZPDN_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297477.1"
                     /translation="MNAKEMFEALGYEEQFKNSDIYRNKKDGSRIAFDNYEGGTIDVV
                     ELCYIPHELLKAINMRCQELGWI"
     gene            complement(4591..4800)
                     /locus_tag="ACZPDN_02270"
     CDS             complement(4591..4800)
                     /locus_tag="ACZPDN_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297478.1"
                     /translation="MTAKEMFYKLGYVRNKRKGSVLYVRGFGDCILFEDRCVKMSDVH
                     GYDRVCNDCELEKAIIQQMKELGWS"
     gene            complement(4797..5288)
                     /locus_tag="ACZPDN_02275"
     CDS             complement(4797..5288)
                     /locus_tag="ACZPDN_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297479.1"
                     /translation="MNKYKEAIKVVDTVLHHMCGEEREDGYKHTMEEMSKSMDLLTDL
                     ANKADAFNELKYSNHRFTLEEYRESRIGKECANRPLEDILYAVTSIYEDEFMYASKDL
                     ERVKNNLKEWLDADVPKERWNEEYRKMAMSIYEFKRRKDLFEYFLGSIEWIKNQYEVV
                     KRR"
     gene            complement(5301..5648)
                     /locus_tag="ACZPDN_02280"
     CDS             complement(5301..5648)
                     /locus_tag="ACZPDN_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297480.1"
                     /translation="MTSQLIINRLTKAKRKFDVCEHHRHAILYCKKCGVKLGEYDLIY
                     DSHESHALCGNCANEYKKEVPIVLNDNVTVTSDCGNFVKVFYEDTQQYEIKQCYFTRK
                     GRYFNYHGKRLYV"
     gene            complement(5641..6063)
                     /locus_tag="ACZPDN_02285"
     CDS             complement(5641..6063)
                     /locus_tag="ACZPDN_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016343.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HIR1297481.1"
                     /translation="MKMNKYQIALNEISSAVLDVYADGYNQQRTVQECYCSTFETLQE
                     LVDKSDSFEWISVNEKLPEEHDSIFAKLYGTDKWDSKLHRTVSNRVLVTVQCDDGTRF
                     VKESHTRDGEWADEKKCINCKVVAWMPLPSPYKEENND"
     gene            complement(6050..6349)
                     /locus_tag="ACZPDN_02290"
     CDS             complement(6050..6349)
                     /locus_tag="ACZPDN_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297482.1"
                     /translation="MRNKIEWRYNDETETYELYHLTYTRTETLIASIRKGSEKYYCNF
                     KCPNVLIEDDCPLDEDLKSSKLHAQGALIEDALAEIEALKDLIRALGGEIDDENE"
     gene            complement(6339..6653)
                     /locus_tag="ACZPDN_02295"
     CDS             complement(6339..6653)
                     /locus_tag="ACZPDN_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297483.1"
                     /translation="MNEYKKALNTIGDTLTYYMVGEDLASLHSVNEIGDSMATLRELV
                     DKETPKKSIDIEFDPCGDLMLCCPNCHHGVVPIPTYNGNKYYPRCPFCGQKLKEGDED
                     EK"
     gene            complement(6655..6828)
                     /locus_tag="ACZPDN_02300"
     CDS             complement(6655..6828)
                     /locus_tag="ACZPDN_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297484.1"
                     /translation="MNSKHAERWEELRKVNPNLAREYLRIYTTMDKAQKHFNNFKKEC
                     NDWLDNIYKDGVK"
     gene            complement(6821..7081)
                     /locus_tag="ACZPDN_02305"
     CDS             complement(6821..7081)
                     /locus_tag="ACZPDN_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297485.1"
                     /translation="MIRLKNKYAITSSGGSFALVTFVKGKDKEGNEIDVQKPISYHTT
                     LESALQSYSNNRMADLVSEVDMDLKDVKRAINELKEELKAYE"
     gene            complement(7074..7322)
                     /locus_tag="ACZPDN_02310"
     CDS             complement(7074..7322)
                     /locus_tag="ACZPDN_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297486.1"
                     /translation="MLEVKKLKDFMEERNIEDGEEFDQIPLTDWFVFEDCFGYIIGLF
                     DSQELNEDYIVLSDTPKVTFEDVKKKYHFENGEVVEND"
     gene            complement(7334..7747)
                     /locus_tag="ACZPDN_02315"
     CDS             complement(7334..7747)
                     /locus_tag="ACZPDN_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297487.1"
                     /translation="MEIKEGRLGLVEGHAVAFDFDGVIHKYREGWKDGSIYDEANKEA
                     IELMLLMKLSGIPVVIISTREPKQIKEWWDKQGFVLEAKVLGKKKMFHKTCDYVGITN
                     RKIPAQLYIDDRAYKYDGQTAKQVIKDLTFFTELN"
     gene            complement(7747..7917)
                     /locus_tag="ACZPDN_02320"
     CDS             complement(7747..7917)
                     /locus_tag="ACZPDN_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297488.1"
                     /translation="MTPYEFTEINRKYNFQTTFNSKLKAMSKDEYEEMVKGLVLVFKA
                     IYNDDSNEEDDF"
     gene            complement(7920..8210)
                     /locus_tag="ACZPDN_02325"
     CDS             complement(7920..8210)
                     /locus_tag="ACZPDN_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297489.1"
                     /translation="MKLIELLPLIESSTVNVYEKRRYGPPRFIVLINPKKTKGCISDD
                     LLDREIYKISAGYNSKFNIYVCNKKDDEELIKKAASIGLIEDVNNDDLDEDV"
     gene            complement(8207..8443)
                     /locus_tag="ACZPDN_02330"
     CDS             complement(8207..8443)
                     /locus_tag="ACZPDN_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297490.1"
                     /translation="MWIRSQNKRILSKVNEVLISTMDDNKYFIEGFWDRGSDTLGIYS
                     SESKALMVLDKIESYLEYSHNVVFQMPQDDEVGL"
     gene            complement(8445..8942)
                     /locus_tag="ACZPDN_02335"
     CDS             complement(8445..8942)
                     /locus_tag="ACZPDN_02335"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020341.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR1297491.1"
                     /translation="MTSTELIKDMLERQKSYDAEVSKKHNVDYVSKSQLESALFDELG
                     ELMHAQKSDWCWWKFTQEPKDEAKVFEEYIDVVHFALMYEIKFGSGCYQDEDIKWNYN
                     KLKTDLGFGQAYAFSCVISLTRDDNVLAYVIALGLHLGYSIEEIYNEYISKNEINYKR
                     LKEGY"
     gene            complement(8953..9273)
                     /locus_tag="ACZPDN_02340"
     CDS             complement(8953..9273)
                     /locus_tag="ACZPDN_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297492.1"
                     /translation="MENNTVTLDEGITINTVIEETEDNFNVLQVEAGSTGKCGGDSGH
                     GCRTYFRIQDVASTDMTINKIADRFGFVSGFEVILGGDSELDSIIEALKFIVDTLEIA
                     KKNS"
     gene            complement(9284..10174)
                     /locus_tag="ACZPDN_02345"
     CDS             complement(9284..10174)
                     /locus_tag="ACZPDN_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HIR1297493.1"
                     /translation="MTENDLLLFDRIEVIKKTIEKYGEENFYISFSGGKDSTVLHHLI
                     DEAIPDNKIPRVFINTGIEYNEIRRFVEKLTENDCRFVIFNSGVNIKEMLNEKGYPFK
                     SKEHSCKLSMFQKKGTETKSVSKYINEKSFGCPEILKYQFTDEFDLKVSDRCCYELKK
                     HPIQRYEKESGRNISILGLRIGEGGQRANHEGCVVFNNNHELKKFKPLNPCSNEFIEW
                     YIQRNNIKLCKLYYQPYNFKRTGCVGCPYSVDLQDQLEIMDKYFPNERKQCELIWKPV
                     YEEYRRIGYRLKRVEEKRLF"
     gene            complement(10317..10652)
                     /locus_tag="ACZPDN_02350"
     CDS             complement(10317..10652)
                     /locus_tag="ACZPDN_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297494.1"
                     /translation="MLENNVYGRYATFFKDHELKEADEYIKRVFHDAEFYIDYEHALV
                     FERIGEDEEIDLDYHTVINGIAYNAAFTNSHKDLVEFYDEVEIKNDKEKKSKVFTCNG
                     KKYEQETLF"
     gene            complement(10639..10842)
                     /locus_tag="ACZPDN_02355"
     CDS             complement(10639..10842)
                     /locus_tag="ACZPDN_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297495.1"
                     /translation="MIAKRYDDELIYSVMKCEGDNKYKYCTKDGKLAFKKPGNDFLGA
                     TKENIMNLYVIEGSLYIGEYVGK"
     gene            complement(10843..11301)
                     /locus_tag="ACZPDN_02360"
     CDS             complement(10843..11301)
                     /locus_tag="ACZPDN_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1297496.1"
                     /translation="MNRVILSGEIGSDIVLKKTATGQSLCNFSIEVKEKGKDGQEHKS
                     FFDCTAWGENAEHINQYGFRGQHIAVDGKLQKSSYTNKENQKVYKTSVYVMDVELALN
                     NATMPQTKAYQQAYQPQSQQMQKPQTVPFTNQVNYQSYPEHHDLDEGMPF"
     gene            complement(11317..11961)
                     /locus_tag="ACZPDN_02365"
     CDS             complement(11317..11961)
                     /locus_tag="ACZPDN_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297497.1"
                     /translation="MQYYMLDKNDISVVRGIVSSKDIMKELGITNAQFHKMLRNEETY
                     KGCILLTVETDEEERRKVTSQDDEQFQLLGESKTGIRYYITSYLRVVSVDLKGNQKEM
                     KAKKETESIYRVVVNFKEGKRYLNVLFEAYKAFVGEIEKNDSIVWDGEMKIENLRVIK
                     LAQTQGLRNKKKVRIGDTVYSSIAECARKNFISKSHMYQMIEGIRPNSIGVEFV"
     gene            complement(11980..12558)
                     /locus_tag="ACZPDN_02370"
     CDS             complement(11980..12558)
                     /locus_tag="ACZPDN_02370"
                     /inference="COORDINATES: protein motif:HMM:NF022858.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative helicase loader/inhibitor"
                     /protein_id="HIR1297498.1"
                     /translation="MTLEETERILQVLRINYPMSYKNMTQEDTQAYLKLWQVSFKDYE
                     YLVVAKAVNQIIQSDTREFAPNVAQVKTRISKTAIGKTKEAGEAWEIVLRNAKCDPHT
                     SKVNYDKLPRNIQKALGGSYLLRDIAWSNKKDLQYYRDRFLQAYKEICEEEVQLLNSG
                     QISLEMYQQHDQLPAPPKKEEGMKMLGELMNG"
     gene            complement(12555..13289)
                     /locus_tag="ACZPDN_02375"
     CDS             complement(12555..13289)
                     /locus_tag="ACZPDN_02375"
                     /inference="COORDINATES: protein motif:HMM:NF025107.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297499.1"
                     /translation="MSAIRVNNTKGFTVMSNYHFQDKEISLKAKGLLGLMLSLPSNWD
                     YSVNGLVAIVKENKAAVQTALKELEEHKYLKRTRVQDETGRFDYIYDIYEKPYDKLPC
                     TENQCTDIQCTEVQCTENQPQINTNKQNTNKQSTKELNTNEYKEKNIKKESVNSVIAE
                     YTENKDLQDALHDFVDMRTKARKPLTVRAMKLSLNELDKLAVDDVTKIAIVNQSIVHS
                     WLTFYKLQNSNGGQRQLTRKEMGYAF"
     gene            complement(13301..13687)
                     /locus_tag="ACZPDN_02380"
     CDS             complement(13301..13687)
                     /locus_tag="ACZPDN_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024784.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR1297500.1"
                     /translation="MKDALELKDYREKFKRYYGLSFSKDYHIHHIDLDHSNNDIENLM
                     IVPKELHEVYHECLVNVQMLTEGKGTSRLFEFDVKPTGNISGYQNFVFTSLLRFMKVL
                     EEMNNWYDYKLYLEGKMPNYHGITLH"
     gene            complement(13700..14194)
                     /locus_tag="ACZPDN_02385"
     CDS             complement(13700..14194)
                     /locus_tag="ACZPDN_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297501.1"
                     /translation="MILGIDPANEYSAFVVVENDLSAVVDKGKIPNKELQDKISNWKA
                     ENYPIDYVAIEGIQSFGMPVGQTTFETCYFIGRLLEQFEAFDIEPTLIYRSEEKMTLC
                     HSMKATDATIRQALIDLFAKDTPNKGKGTKKEPGYFYGFKADIWSAFSVAITFHTKYI
                     GTEC"
     gene            complement(14196..14675)
                     /locus_tag="ACZPDN_02390"
     CDS             complement(14196..14675)
                     /locus_tag="ACZPDN_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297502.1"
                     /translation="MLSQNELLFKYNPFKIKHWKDEEIEEQLGILVDAYISDAETVME
                     MALNIENLANQMFLIGEMIARLQESSNILKADIENKTNQEIYVARSTWEREHDGKAPS
                     IKYFEALAGQKVSDERTKFAKVDSDLKRFKTAYESIEAKMNSEKKKLDATKFEIGGA"
     gene            complement(14659..14970)
                     /locus_tag="ACZPDN_02395"
     CDS             complement(14659..14970)
                     /locus_tag="ACZPDN_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR1297503.1"
                     /translation="MYKSKRSQATDIPKSVKDTVWERDGRMCIFCGSPFAFPEAHVIP
                     RSQSGLGVEKNIITVCRRCHNLLDQSPKREKMLGIAKRYLERIYGHIDESEVKYNAKS
                     K"
     gene            complement(14963..15691)
                     /gene="bet"
                     /locus_tag="ACZPDN_02400"
     CDS             complement(14963..15691)
                     /gene="bet"
                     /locus_tag="ACZPDN_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HIR1297504.1"
                     /translation="MLQNNIAKKNDNQLVEFSANGEKVKLSPSIVRNYLVNGNGQITD
                     QEVVYFINLCKSQGLNPFIKDCYLIKYGNTTPAQMVVSKDVFLKRAERNSEFDGLDAG
                     IIVINNESGELTYRKGAFYLKDREEVVGGWADVFRKNVSHPTHIEVSVEEYAGRTKDG
                     KLNSQWASKMATMVRKVAITQALRETFPNDFQQMYSEEEMNVDMKLDETPIQQPTQQI
                     EQAPVQPQTYSQPDEPQPEGVSLV"
     gene            complement(15694..16548)
                     /locus_tag="ACZPDN_02405"
     CDS             complement(15694..16548)
                     /locus_tag="ACZPDN_02405"
                     /inference="COORDINATES: protein motif:HMM:NF018751.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HIR1297505.1"
                     /translation="MEFNLEVRAQNGKVYTNASDLLPEIKEGLKHYNYVVDEGNYKKA
                     KTDRAALNNLVKLVSDKRKQVENDVFAQWLQDKKDIMQVEKTIKAASDKLGDGINEVD
                     NAEKELKRNQIKELWTNMTNDKYPFDLVFEDRYLNKSVKPKEIEESLNNKFLKAEEQL
                     SFIEASLPEDELQAEQVIQLFCKTLDLSKATERINEIKEAKAKLQAKVNAQIEQSKQA
                     QMERENVVPKQAPFETPQTQNQTEPRRYCVFRFEGPMSELQAFNPILNQFIKEHDVKV
                     SILEKGEC"
     gene            complement(16568..17239)
                     /locus_tag="ACZPDN_02410"
     CDS             complement(16568..17239)
                     /locus_tag="ACZPDN_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HIR1297506.1"
                     /translation="MNLYQDTEKFSVEKYESHEEWLKKRGRGIGGSDAACFMDLNPWK
                     TLNQLWHDKKFGSQHITNDAIEYGNTAEPCLRTLFQAKHPELDVQYVDNVTLVSKEHE
                     FLRYSPDGLIYNKETGERGILEIKTSKIINSHSLQKWGSKGNETVPDNYYCQTLEGLI
                     VTDFDFVIYCAELRFSDGDARIIERSYRKEEALDSMNDLKQAMIEKWDRYFVNDIEPP
                     ITLSI"
     gene            complement(17217..17456)
                     /locus_tag="ACZPDN_02415"
     CDS             complement(17217..17456)
                     /locus_tag="ACZPDN_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025456.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297507.1"
                     /translation="MTQTERVIKNLKEYGSITTLEAIREYGITRLGARIWDLRDLGYD
                     IETQTETSKNRFGDKTSYAKYVLKGEVKNESLPRH"
     gene            complement(17681..17824)
                     /locus_tag="ACZPDN_02420"
     CDS             complement(17681..17824)
                     /locus_tag="ACZPDN_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297508.1"
                     /translation="MMDVDDLRELDDNRYIDEDEEEEQDEYSYEDYCYDFCKAERDEE
                     AWF"
     gene            complement(18017..18220)
                     /locus_tag="ACZPDN_02425"
     CDS             complement(18017..18220)
                     /locus_tag="ACZPDN_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297509.1"
                     /translation="MAEPSGRLESDRLDSIRLFQDSVHLEGKVLDLLIKNGCSNEDLL
                     NVSSMLQTIYMCGFEVGKRCVKE"
     gene            complement(18274..18459)
                     /locus_tag="ACZPDN_02430"
     CDS             complement(18274..18459)
                     /locus_tag="ACZPDN_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1297510.1"
                     /translation="MELTIKQIRVGLNMTQKEMAKYLGISTVSYQRKESGIKNFYFWE
                     VKKICSKANVSLDIVKI"
     gene            18640..>18829
                     /locus_tag="ACZPDN_02435"
     CDS             18640..>18829
                     /locus_tag="ACZPDN_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1297511.1"
                     /translation="MGNKFESQGQVLKKFRKECGFTNQEVVDRVGKSPMWLSDIENGK
                     SNLFFKDAKKLCHIYGHTL"
BASE COUNT         5282 a         3807 c         2388 g         7352 t
ORIGIN      
        1 atgagaatta atacaagtat agttgtaagc tgatcaatca tctatcaacc tcttttttat
       61 ccccacatag tgccaaatcg tatacactca tttaatggca cctaccaatc aattttgata
      121 ctagtaagaa cataatccca actagtgcca tagtgagtta cgccccaaag atacatattt
      181 agcgtttcgt tataacagac aatttgattg atatctttta ataatctagc accccaatcg
      241 tcaacgatat accattggaa gacctcttga tcgtcttgtt ctctttcaag ctcttctatc
      301 tcgtcggtat tttcaatagt tccgctgact tgttcccacg atcctatatc atatgtcaat
      361 tgcatgatgt cgttatttaa tacagcgtca aaacattttg caagagtagc atagtcaact
      421 cgcccatttt ctaatccgta tttgctgatc gcatttccgt gataatattt ttgttccatt
      481 ttcttttttg ccccatgtgt tataattatg acgtatatat atatttttgg ggcccgcctc
      541 cattttctat tctataatta atatatatat acgttttctt tggatgatcg tttagttgtc
      601 agctggtacg atcatctttt tttatgtctt atttttggac gtcacccctt ttctacaata
      661 ctattatatc acgatatcgc tatataatcc accatttttt caatttttgt tcacgaatcg
      721 tgaatataat agcggaaatt tttataccaa cccgtatttt tgtctacaaa tcgtgaacaa
      781 tttacaaaat acaaaatata caaaatacaa aatttttcgc cgattacaaa atacaaaata
      841 caaaatttat tttttatatt gacaaaattt tctttttgga acacttcaaa tcccctttaa
      901 ataaaggata ttattctttt ttcatgaaca attgttaatt caaacaaaaa aagcaataaa
      961 aaaacccatc aaactacatt agaacttgat aggtaaatga aactagtaat aatagtatat
     1021 atatcttatt cttatagggt ttggaaggct ctgtggagga cgtagctcct caattcttct
     1081 ttcccccgaa gtcaaaaccc ctctttatct cccccaaacc tctttcctat tatatatatg
     1141 ccgagggact tagatatatc agcttttttc aaagatcaat aattttaaaa tatgcagaac
     1201 tgtttataat ataggctctg ttattacaga accttaaacc atacaatcga caagatatag
     1261 aattaataaa gaatttctaa aggagtatga aatgaaatat ctgcctattc attccagtag
     1321 tagtatatga ctacgtgatg acaatttatg aaccaactta aaccaatttt gtgtaaacat
     1381 tagtgataac attatggaac ttatcgtgtt tattacgcat atttgattgt atggacgttt
     1441 ctatatctct gaatttactt ttttttcttt tcccccgacc tctgttgcga tataaggcca
     1501 cgtttcacga aaatcctgtt gcgattgtat ggatgctgcg aatcatgggg ccattgaaga
     1561 atacggggca gtttcattgc acttataggg cgtaattttc tatttcaccc tccccgtcaa
     1621 ttaaatattg cacttacacc gttataatat cctcaggtct ccccgaggca ctcaattctg
     1681 agtaagtaga agtaaaaaat gtctcgttag aaagtggcaa tatttggtct ttttaggtac
     1741 ttcctaacta catatattat accatttttc aatgcggacg tgttaaaaaa atacgataaa
     1801 aaaaggctat ttgttgttag cctctttctt ttctctttct aaatcctgta aaatcagttg
     1861 tcttacataa ttgttcttac taggtacaga atcaagcttt tctatgatct ctgcttcatg
     1921 agttttatga aattttagaa gaatttgtct gatatgtttt ttttcatact tcctatttgc
     1981 tcgcatcttt gcttcgcttg tttttgccat tgtttactcc tcctattcgc tatacataaa
     2041 agcgaaacta tcactaatct tttcttttct ttcaatgtaa ccaatcaaga aatcccattg
     2101 tctgcaagat attacatcat ctttgtaata tctgcctaca atttctttta gttcttcttc
     2161 actgattaca tccatctttt catttacttc tttgcataca ttttgtaatt gttttaaatt
     2221 tgtcatattg tgttctcctt taattacttt tctttacacc catatattag catataacga
     2281 tatctttgta aataattaat ttaataaatt tttaaattct ttttcataat aaaaaaggct
     2341 ataaacttgt ttatcgtttt aaacgtgttt ttagcctttt ctttatttta cctaacaaat
     2401 actcatataa atctattttg ctcgttagaa taacttctag acgtgtttaa attgatttta
     2461 agaagttttt cttctttttc tacgcaagtt gtactcttta tcaatcaaaa tctgtaatat
     2521 cattgttcta tcaactaaat attcaattcc atcactattg aaaccaacga tcttacacca
     2581 ccagcgattg tatgtataag gtttagtcaa tacgatttgc actaattcgg tttcatcaaa
     2641 taaagtagcc attgcgatat cacctgctct taagccaata ttgccatggt aattaaacca
     2701 tcctccgcag gtttctttga aatgctcgta ttctgtatct cttctaggca ttatacaatc
     2761 cttaaataat tcccatttga gttaataatg cacattttac ttctttttct tctcgtctat
     2821 cgagtggttt aatatgccac ttaacatttt ctttgttgat cgttaagatt tgttcggctt
     2881 ttgccatacc gtactcgtgc cccgtatcaa tcataacgtg acatggcaaa tctgtccttt
     2941 ttaaattgct tgtaattgga attacattca ctgttttact tcctttgttc tgaatatcgt
     3001 ttgaaataac gatacaaggt ctccttttgc aaagtactgt attgctatat cttggcaaat
     3061 cacaccaata gatattattg tttaggattt ccataatgat aacctcctat ccttcctaat
     3121 ttatcctcta aatttctgtt atgctgctgc aatccgtata tctttctatc tctctcaatt
     3181 aaagcctgtt taattaattc catatcatca tatgcttcat ataatccgtt gtcttttaga
     3241 gttttttcta tgtttttaaa actcgtctct acttgagttg ttgctatcat cttcttcatt
     3301 ccaatccatc ccgtacacgt cgtctactgc atcatcatca tcttccttga ttggaacacg
     3361 tacaactcta gttccaattc tatgcataaa taaaatacat attgcccaaa taggatgtac
     3421 atgaattacc atgtatgcag taaataccat taacgctata ttatgaattg ctatacttaa
     3481 atacatcatt ttgctatttt ctccttcata tatgtagctt tcaaattttc tacttcgacc
     3541 ccttctttac tccatgcagc atcccaaatt tcatttaata atgaatctac aacattgcat
     3601 gaatcactgt tattaacatc aggaacactg attttcaatt taatcagtac gtctgtactt
     3661 tctttaggct tatttcgttt cttcaaaccc atcataccca tatctcctat gttgatattt
     3721 gtattttcta acttcatatt ctgctttatt aagatcatca atcaacctac aattttcaag
     3781 ttccaattca ttaattcgct ctgaaacaat taatgaataa agaagcattg aagctatgcc
     3841 acctatgaaa aatcctgcaa cgaaataaat catccttcta cctcacaatt tgctaggatg
     3901 tcttcaatcg ttgcatcctt atcaacgtct ttgaaatgcc ctttttcttt catcaaggtt
     3961 aaaacattta tatttttaaa cctgaaatct tttattgaat tgatttgcaa taaatcgact
     4021 tcaaatttag ttagtttaat tgcggttttt ttatagggct gctctagcca ttcccttacc
     4081 tttctatggc atcctcttag aatattttta aagtcacagt cactgcaatt tgttttgtta
     4141 cattgcctag gtattccttt gactaccgct aaatcccacg tgcaagcttc tagaatttca
     4201 tgtttgtaat gatctagatt agtctctttc ttttcatctt gcttttcatc aaaatgttca
     4261 tttactaatc ctgcaagtaa atttatatct tctttgaatt tgttcattga actcatacaa
     4321 ccatcaaaat tgtaatatac ttgttctata cggtttaaag catctatata ttcttgtttt
     4381 gttttcacta aatccacccc aattcttgac atcgcatatt tatcgctttt aataattcat
     4441 gtggtatata acataattca accacatcta tagttccacc ttcataatta tcaaatgcaa
     4501 ttcttgaacc atctttttta tttctgtaaa tatcactatt tttaaattgt tcttcatatc
     4561 ctagtgcttc aaacatttct tttgcattca tcatgaccac cccaattctt tcatttgttg
     4621 aattatggct ttttctaatt cacagtcatt gcaaactcga tcatatccat gtacatcaga
     4681 catttttaca catctgtctt caaagagtat acaatctcca aatcctctaa cgtatagtac
     4741 agatcctttg cgtttatttc tgacgtaacc taatttgtaa aacatttctt tagctgtcat
     4801 cttcttttca ccacctcata ctgattttta atccattcaa tactacctaa aaaatactca
     4861 aataaatctt ttctacgttt aaattcgtat atgctcatgg ccatttttcg atactcttca
     4921 ttccaacgtt cttttggaac gtcagcatct aaccactctt tcaaattatt tttaactctt
     4981 tctaaatcct tactagcata catgaattca tcttcatata tcgatgttac ggcatacaat
     5041 atatcttcta gtgggcgatt ggcacactct ttgcctattc tgctttctct atattcttct
     5101 aacgtaaatc tatgatttga gtatttaagt tcattaaatg cgtctgcttt attagctaaa
     5161 tctgttaaca aatccataga tttagacatt tcttccatag tatgcttata tccatcttct
     5221 ctttcttctc cacacatatg atgcaataca gtatctacga ctttaatagc ttctttatat
     5281 ttattcatta tctttctcct ttagacatac aatcttttac cgtgataatt gaagtaacgt
     5341 ccttttcgag tgaaatagca ttgcttaatt tcatattgct gagtatcttc ataaaacact
     5401 ttaacaaaat taccacaatc acttgtaacc gttacattgt catttaatac aattggaact
     5461 tcttttttat attcatttgc acaattacca cacaacgcat gagactcatg cgaatcataa
     5521 attaaatcat attcgccaag ctttacacca cattttttac aatatagaat tgcatgtcta
     5581 tgatgttcgc atacatcaaa ttttcttttt gcttttgtta atctattaat aataagttgg
     5641 ctagtcattg ttttcctcct tatacggact aggcaatggc atccaggcaa caactttaca
     5701 gtttatgcat tttttctcat cagcccattc accgtcacga gtatgagact ccttgacaaa
     5761 tcttgtgcca tcatcacatt gaacggttac aagcactcta tttgatactg ttctatgtaa
     5821 tttactatcc catttatctg ttccgtacaa tttagcaaag atactatcgt gttcctctgg
     5881 cagcttttca ttgacagaaa tccactcaaa tgaatctgat ttatcaacta actcctgtaa
     5941 tgtttcaaac gtactgcagt aacattcttg tactgttctt tgttgattat atccatctgc
     6001 gtatacatcc aacactgcac ttgaaatttc attgagtgct atttgatatt tattcatttt
     6061 catcgtctat ttcgcctcct aacgcacgaa tcaaatcttt caatgcttca atctcagcca
     6121 atgcatcttc aataagtgct ccttgtgcat gaagttttga actctttaag tcttcgtcta
     6181 gcggacaatc atcctcaatc agtacattag gacatttaaa gttacaataa tacttttcac
     6241 ttccttttcg tattgatgca attaaagttt ctgttctagt atatgttaga tgatacagtt
     6301 catacgtttc tgtttcatcg ttatatctcc attcaatttt atttctcatc ttcatcgcct
     6361 tcttttaact tctgtccaca aaatggacaa cgtggataat atttatttcc attataagtt
     6421 ggaattggca caaccccatg atggcaattt ggacaacata acatcaaatc accacatgga
     6481 tcaaactcaa tatctataga tttcttaggt gtttctttgt caactaattc tctaagtgta
     6541 gccattgaat cacctatttc attgacacta tgtaaagaag ccaaatcttc acctaccata
     6601 taataagtaa gagtatcacc gattgtattt aatgcttttt tgtattcatt cattttattt
     6661 cactccatcc ttataaatgt tatctagcca atcgttacat tcttttttaa aattattaaa
     6721 atgtttttga gctttatcca ttgtggtata aattcttaaa tactcccttg ctaaatttgg
     6781 gtttaccttg cgtaattctt cccatctttc tgcgtgctta ctattcatat gcctttaatt
     6841 cctctttaag ttcatttata gctcgcttaa catcctttaa atccatatca acttcagata
     6901 ctaaatctgc catacgattg ttagaatagc tctgtaaagc cgattctagc gttgtatggt
     6961 atgagatagg tttttgtacg tctatctcat ttccttcttt atccttaccc ttcacgaacg
     7021 ttacaagggc gaatgaacca ccactagaag tgattgcata tttgtttttt aatctaatca
     7081 ttttctacaa cctctccatt ttcaaaatga tatttcttct taacatcttc aaatgtaact
     7141 ttcggtgtat cggacaaaac aatgtagtct tcatttaatt cttgactatc aaataatcca
     7201 ataatatatc cgaagcaatc ttcaaataca aaccaatcag ttaatggaat ttggtcaaac
     7261 tcttctccat cttcaatatt tctttcttcc ataaaatctt tcagtttttt aacttctaac
     7321 atttttcttt ctcctaattt aattctgtga aaaaagttaa atctttaatt acttgttttg
     7381 cagtctgccc atcatattta tacgctctat catcaatata taattgagca ggaattttac
     7441 gattcgtgat acctacataa tcgcaagttt tgtggaacat ttttttcttg cctaatactt
     7501 tggcttctaa tacaaatcct tgtttatccc accactcttt aatttgtttt ggttcacgtg
     7561 ttgaaataat tacaacagga atacctgata atttcataag taacatcaat tcaatagctt
     7621 ctttgttagc ttcatcataa atacttccat ctttccaacc ttcacgatat ttgtgaatta
     7681 ctccatcgaa atcaaacgct actgcatgac cttcgactaa tcctaatctt ccttctttaa
     7741 tttccattaa aaatcatcct cctcatttga atcatcattg taaatagctt taaatactaa
     7801 aactaaaccc tttaccattt cttcatattc atcttttgac atagctttta atttgctatt
     7861 gaatgttgtt tgaaaattgt atttcctgtt tatttctgta aattcgtaag gtgtcattcc
     7921 taaacatcct catctaaatc atcgttgtta acatcttcaa tcaatccaat tgaagcagct
     7981 tttttaatta attcttcatc atctttcttg ttgcaaacgt aaatgttaaa tttactgtta
     8041 taacctgctg atattttata aatttctcta tctaataaat catctgaaat gcatccttta
     8101 gtcttttttg gattaatcaa tacgataaat ctaggtggtc catatcttct tttctcataa
     8161 acgtttactg tagaactttc aatcaacgga agcaattcta ttagtttcat aatccaacct
     8221 catcatcttg tggcatttga aatacaacat tgtgtgaata ttctaagtaa ctctcaatct
     8281 tatccaatac cattaaagct ttagactcac ttgaataaat tcctaatgta tcacttcctc
     8341 tatcccaaaa cccttctata aagtacttat tatcatccat agtgcttatt aatacttcat
     8401 tgacttttga taatattctt ttattctgac ttctaatcca catattaata tccttctttc
     8461 aatcttttat agttgatctc attcttgcta atgtattcgt tatatatttc ttcaatcgaa
     8521 tatcctaaat gcaatcctag tgcgattacg taagctaata cgttatcatc tcgtgttaag
     8581 ctgattacgc agctaaatgc gtatgcttga ccaaaaccta aatccgtttt caacttgtta
     8641 taattccact taatatcctc atcttgataa catcctgaac caaacttgat ttcgtacatc
     8701 aacgcaaagt gaacaacatc aatgtattcc tcaaacactt tagcttcgtc ttttggctct
     8761 tgcgtgaact tccaccaaca ccaatcagat ttttgagcgt gcatcaattc tcctaattca
     8821 tcaaacaatg cactttctaa ttggctttta gaaacgtagt ctacattatg tttcttagat
     8881 acttccgcat catatgattt ctgtctttca agcatatctt taattaattc tgtacttgtc
     8941 atttgtttct ccttaactgt tcttctttgc aatttctaat gtatcgacaa taaattttaa
     9001 cgcttcaata attgagtcta actcgctatc tccaccaaga atcacctcaa aaccactcac
     9061 aaatccaaat ctatccgcaa ttttgtttat cgtcatatct gtgcttgcaa catcttgaat
     9121 tctgaaataa gttctacatc catgtcctga gtcaccacca catttaccag tgctgccagc
     9181 ttcaacttgt aacacattaa aattatcttc ggtttcctct attactgtgt taatcgttat
     9241 tccttcatcc aatgtaactg tattattttc cattttttct cctttagaat aatctctttt
     9301 cttctactct ctttaaccga taaccaatcc ttctatattc ctcgtataca ggcttccaaa
     9361 tcaattcgca ttgcttacgc tcgtttggaa aatatttatc cataatttct aattgatctt
     9421 gtaaatcaac tgagtagggg catccaacgc atcccgtcct cttaaagttg tatggttgat
     9481 aatatagctt gcatagcttt atattgtttc tttgtatgta ccattctatg aactcgtttg
     9541 aacatggatt taaaggcttg aacttcttca actcatgatt gttgttaaac acaacacatc
     9601 cttcgtgatt tgctctttga cctccttcgc caattcttaa tccaagaatg gaaatatttc
     9661 ttcctgattc cttttcatat cgttggattg gatgtttttt taattcgtag caacatctgt
     9721 cgctaacctt taaatcaaat tcatctgtaa attgatattt cagaatttca ggacaaccga
     9781 aactcttttc gttgatatat ttacttaccg attttgtttc cgttcccttc ttttggaaca
     9841 ttgatagctt gcagctatgc tctttagatt taaacggata gcctttttcg tttaacattt
     9901 cttttatgtt taccccagaa ttgaaaataa caaatctaca atcattttct gtcaatttct
     9961 caacaaacct ccgtatttcg ttatattcaa tccctgtatt aatgaatact ctcggaattt
    10021 tgttatcagg tatagcttca tcaattaaat gatgtaaaac tgtactgtct ttccctcccg
    10081 agaaagatat gtagaagttt tcttcaccat atttctcaat cgtctttttt attacttcta
    10141 ttctgtcgaa tagtaataaa tcgttttctg tcaattaatc aaccgataat ctaatttcta
    10201 gactcggtta atttgctact tgattgttaa tccatgtgtt taaattttat gagtgttcac
    10261 ttcatataac actcaaccta gtttcactag gataagatta attttccttt ctgtttttaa
    10321 aacaaagttt cttgttcata ctttttaccg ttgcacgtaa atacttttga tttcttttct
    10381 ttatcatttt taatctctac ttcgtcataa aactcaacta aatctttatg gctatttgta
    10441 aatgctgcgt tgtaagcaat cccgttaatc actgtatgat aatcaagatc aatttcttca
    10501 tcttcgccta ttctttcaaa aactaatgcg tgctcgtaat ctatataaaa ctctgcatca
    10561 tgaaacacac gcttaatgta ttcatccgct tcttttaatt cgtgatcttt gaagaatgta
    10621 gcatatcttc cgtaaacgtt attttccaac atattctcca atgtatagac ttccttcaat
    10681 tacatacaga ttcataatgt tttcctttgt tgcccctaga aaatcatttc caggcttttt
    10741 aaaagctagt tttccatctt ttgtacagta tttgtatttg ttatctcctt cacatttcat
    10801 aacactgtat ataagttcat catcatatct tttcgcaatc atttagaatg gcatcccttc
    10861 gtccaaatca tgatgttcag gatatgattg gtaatttact tgatttgtaa atggaactgt
    10921 ttgaggcttc tgcatctgtt gtgattgagg ttgatatgct tgttgataag ctttcgtttg
    10981 tggcattgtc gcattgttta aagctaattc tacgtccata acgtaaacgc tagtcttata
    11041 caccttctga ttctctttgt tcgtgtatga gcttttttga agctttccgt caactgcgat
    11101 atgttgtcct ctgaatccat attgattaat atgttctgca ttttctcccc aagcggtgca
    11161 atcgaagaaa gatttatgtt cttgtccgtc cttacctttt tctttaactt caattgagaa
    11221 gttacatagg ctttgtcctg tagcagtttt ctttaaaaca atatcgctac cgatttcacc
    11281 tgataaaatc actctgttca tttcttttca actcctttat acaaattcaa cacctattga
    11341 attaggtctg attccttcta tcatctgata catatgtgat ttagaaatga aattctttct
    11401 agcacactcg gcgattgaag aataaactgt atcgcctatt cttactttct tcttgtttct
    11461 taagccttga gtctgagcta gtttgataac tcttagattt tcaattttca tttctccgtc
    11521 ccaaacgata gaatcattct tttctatttc cccaacaaaa gctttgtagg cttcaaataa
    11581 tacgttcaag tatcgtttcc cttctttaaa gttcactaca actctgtaaa ttgattccgt
    11641 ttccttttta gccttcattt ctttttggtt tccttttaag tcaacagaaa caactcttaa
    11701 ataacttgta atgtaatatc tgattcctgt tttactttct ccaagtagtt ggaattgctc
    11761 gtcatcttga cttgttactt ttcttctttc ttcctcatcc gtttcaacag taagaagaat
    11821 acatcctttg tatgtttcct cgtttctcaa catcttatgg aactgagcgt ttgtaatgcc
    11881 caattccttc attatatctt ttgaagatac gattccacgt acaactgata tatcgttttt
    11941 atccaacata taatattgca ctttctaccc ctccctttct tatccgttca tcaattctcc
    12001 caacatcttc ataccttcct ccttttttgg aggtgcaggc aattgatcgt gttgttgata
    12061 catttccaaa ctgatttgac ctgaatttaa taactgtact tcttcttcac aaatttcttt
    12121 gtaggcttgt aaaaatctat ctcggtaata ttgcaagtct tttttattac tccacgcaat
    12181 atctcttaac agatagcttc caccgagtgc tttctgaatg tttctaggca gtttatcgta
    12241 gttgacctta ctagtatgag ggtcacactt agcgttcctt aaaacgattt cccaagcctc
    12301 tccagcttcc ttagtttttc caatagcagt tttactgatt cttgttttga cttgtgccac
    12361 atttggagca aactctcttg tatcgctttg gatgatttga ttcactgctt ttgctactac
    12421 caagtattca taatccttaa aagatacttg ccaaagtttt aaataagctt gcgtatcttc
    12481 ctgagtcatg tttttgtaac tcattgggta attgattctt agcacttgta agattctttc
    12541 agtttcttct aatgtcaaaa tgcatacccc atttcttttc tcgtcaattg tctttgacca
    12601 ccattgctat tctgcaactt atagaatgtt agccagctat gtacaatgct ctgatttaca
    12661 atagcaatct tggtaacatc atctactgcc aatttatcta attcatttaa agacaacttc
    12721 attgctctaa cagtcaaagg ttttcttgct ttagtacgca tatctacaaa gtcatgcaat
    12781 gcatcttgca aatctttgtt ttctgtatac tctgcaataa cagaattaac actttctttt
    12841 tttatatttt tttctttata ttcattagta tttaattctt tagtactttg tttattagta
    12901 ttttgtttat tagtatttat ttgtggttga ttttccgtgc actgaacttc cgtgcattga
    12961 atatccgtgc actgattttc cgtacacggt aatttgtcat acggtttctc gtaaatatcg
    13021 tagatgtaat caaaccttcc tgtttcatct tgaactctag tacgctttaa atatttgtgt
    13081 tcttcaagtt ctttgagtgc ggtttgaact gctgctttat tttccttaac aatcgcaact
    13141 aatccgttta ctgaataatc ccaattgcta ggcaaagaaa gcatcaatcc aagaagtcct
    13201 tttgctttta gtgagatttc tttatcttga aagtgataat tgctcatcac tgtaaatcct
    13261 ttagtgttat ttactctaat cgctgacata ctattctcct ttaatgcaat gtaattccat
    13321 gataattagg catttttcct tctagataaa gtttgtaatc gtaccaatta ttcatttctt
    13381 ctagaacctt cataaatcta agtagacttg tgaaaacaaa gttttgatag cctgaaatat
    13441 ttcctgtagg ttttacatca aattcaaata atcttgatgt accttttcct tccgtcaaca
    13501 tttgcacgtt tactaaacat tcgtgatata cttcatgaag ttcttttgga actatcatca
    13561 agttttctat atcattgttt gaatgatcta agtcaatatg gtgaatatga taatctttgc
    13621 taaaacttaa tccgtaatat cgtttgaatt tttcacgata atcttttagt tccaatgcat
    13681 ctttcactct taacacctcc tagcattctg tacctatgta ctttgtatgg aacgttattg
    13741 cgacactaaa cgcactccag atgtcggctt taaatccgta gaaatatcca ggctcttttt
    13801 ttgttccttt tcctttgttt ggggtatctt tagcaaacaa atcaattaac gcttgtctaa
    13861 tagttgcatc tgtcgctttc atagagtggc atagagtcat tttttcttca cttcggtata
    13921 ttaatgtggg ttcaatatcg aaagcttcaa attgctctaa taaacgccct atgaagtaac
    13981 aagtttcaaa tgttgtttga cctacaggca taccgaaact ttgtattcct tcaatcgcca
    14041 cataatcaat tggataattc tctgctttcc aatttgagat tttatcttgc aattctttgt
    14101 taggaatttt tcctttatct acaactgccg ataaatcatt ctcgactaca acaaatgcac
    14161 tgtattcatt tgctgggtca atgcctaaaa tcatcttacg cacctccgat ttcaaactta
    14221 gtagcatcaa gttttttctt ttctgagttc atctttgctt caatactttc gtaagcagtt
    14281 ttgaaacgtt ttaaatcaga atcgactttt gcaaacttag ttctttcatc agaaactttt
    14341 tgacctgcta aagcttcaaa gtatttgata ctaggagctt tcccatcatg ttcacgttcc
    14401 caagtgctac gtgcaacata aatctcttga tttgttttgt tttcaatgtc tgctttaaga
    14461 atgtttgaac tttcctgtaa tctagctatc atttcaccaa tcagaaacat ttgatttgcg
    14521 aggttttcaa tgttcaatgc catttccatt actgtttctg catctgaaat ataagcatca
    14581 actaggattc ctaattgttc ttctatttct tcgtccttcc aatgtttgat tttgaatgga
    14641 ttgtatttaa acaacagttc attttgactt agcattatat ttcacctctg attcatcaat
    14701 atgtccgtag atacgttcta aatatctttt ggcaatgcct agcattttct ctctctttgg
    14761 actctggtct agtaggttat ggcaccttct acatactgta ataatgtttt tctctactcc
    14821 aagtcctgat tgagatcttg gaattacgtg tgcttcggga aatgcgaagg gagaaccgca
    14881 aaagatacac attctcccat ctctttccca tacagtatct ttaactgatt taggaatatc
    14941 tgtagcttgg ctacgttttg atttatacaa gacttacacc ttcgggttgc ggttcatcag
    15001 gttgtgaata tgtttgtggt tgtacaggtg cttgttcaat ttgttgtgta ggttgttgaa
    15061 ttggagtttc atccaatttc atatccacat tcatttcttc ctctgaatac atctgttgga
    15121 aatcgttagg gaatgtttct ctcaatgctt gagtaattgc aactttacga accattgtgg
    15181 ccatcttaga cgcccattgt gagttaagct ttccgtcttt agttcttcct gcatactctt
    15241 caacagaaac ttcaatatgc gttggatgtg aaacattctt tctaaataca tctgcccatc
    15301 cacctacaac ttcttcacga tcttttaagt agaaagcacc ttttctgtat gtcaattcac
    15361 cactttcgtt attaattacg ataattcctg catctaaacc atcaaattct gaatttcttt
    15421 cggcacgttt taagaaaaca tctttagaaa ctaccatttg agctggtgta gtgtttccgt
    15481 acttgattaa gtagcaatct ttaatgaatg gattcaatcc ttgtgattta cacaaattaa
    15541 tgaaatacac aacttcttgg tctgtaattt gaccattccc atttactaga taatttctta
    15601 cgatagatgg agataattta actttttctc cgttggcaga aaattctacc aattgattgt
    15661 cattcttttt tgcaatattg ttttgtaaca taattaacat tctccttttt ctaaaattga
    15721 tactttcaca tcatgttctt taataaattg attcaaaatc ggattaaaag cttgtaattc
    15781 gctcataggg ccttcaaaac ggaatacgca gtatcttctt ggctctgtct gattttgagt
    15841 ctgaggcgtt tcaaatggag cttgtttagg aactacattt tctctttcca tttgagcttg
    15901 tttagattgc tcaatctgag cattcacttt agcttgaagt tttgctttgg cttccttgat
    15961 ttcattgatt cgttccgtag ctttgcttaa atccaaagtc ttacagaaca attggataac
    16021 ttgctctgcc tgtagttcat cttcgggaag tgaagcctcg ataaaagata attgttcttc
    16081 ggctttcaag aacttgttat ttaagctttc ttcaatttct ttaggtttaa cagatttgtt
    16141 caaatatcta tcttcaaaca ctaaatcaaa tgggtactta tcatttgtca tgtttgtcca
    16201 tagttctttg atttgattac gcttcaattc tttttctgcg ttatcaactt cattgattcc
    16261 atcacccaat ttatcggatg cagctttgat agtcttttcg acttgcataa tgtctttctt
    16321 gtcttgcaac cactgagcaa atacatcatt ttcaacttgc ttacgcttat cagatacaag
    16381 ctttaccaaa ttgtttaaag cagctctatc tgttttagcc ttcttgtagt ttccttcatc
    16441 tactacatag ttgtagtgct ttaaaccttc tttgatttca ggtaataaat cacttgcgtt
    16501 tgtgtacact tttccgtttt gtgcacgtac ctctaaatta aattccatat ttccatctcc
    16561 tctttttcta tatagacaat gtgataggtg gttctatgtc attaacaaag tacctatccc
    16621 atttttctat cattgcttgt tttagatcgt tcatactatc tagtgcttct tctttacgat
    16681 atgaacgctc aatgattcgt gcatcaccat ctgaaaatct aagctctgca caataaataa
    16741 caaagtcaaa atccgtaaca atcaatcctt ctaaagtttg gcaatagtaa ttatcaggca
    16801 ctgtttcgtt tcctttactg ccccattttt gcaaactatg agaattgatt atcttagaag
    16861 ttttaatttc taagattcct ctttctcccg tttccttgtt gtaaatcaat ccatcaggac
    16921 tgtatcttag aaactcatgc tccttagaaa ccaatgttac gttgtccacg tactgtacat
    16981 ccaactcagg atgtttcgcc tgaaacaatg ttcttaaaca aggctctgct gtatttccat
    17041 attcaatcgc atcatttgta atatgttgtg acccaaattt tttgtcgtgc cacaactgat
    17101 taagtgtttt ccatggattc aaatccatga aacaagctgc atccgaacca ccaattccac
    17161 gtccacgctt tttcaaccat tcttcatgac tttcatactt ttcaacacta aacttctcag
    17221 tgtcttggta aagattcatt tttcacctct ccttttaata cgtacttggc atatgatgtt
    17281 ttatctccaa accgattttt agaagtttca gtttgagttt ctatgtcata tcccaaatct
    17341 cttaaatccc aaattcttgc acctaaacga gtgattccat attctctgat agcttctaaa
    17401 gtagtgatag aaccatattc ctttaggttc tttatgactc tttctgtttg agtcatttta
    17461 caaacctctt atgtaccaat tagctatcac aatgaaagct agagaaacta agaaacaaat
    17521 aagagaacaa atgtaattga acttagcagc tcgattaacc atatgagtct gcttttgact
    17581 tctaactagc attgaatact gagtttcgta ctcgttatta gcaaaagaag gaagcgtgat
    17641 acaatcacct aactcaactg ctttcttctt tgcggttgat ttagaaccat gcttcttcgt
    17701 ctctttctgc tttgcagaag tcgtagcagt agtcttcgta actgtactca tcttgttctt
    17761 cctcctcatc ttcatcaatg tatctgttgt catccaactc tcttaaatca tctacatcca
    17821 tcatgttgtt cacacctttc tttgaactca ggaaacatcc tgataaataa ctttgttgga
    17881 actttctttg tatctatcac tttggataga tttgattttt tataagcctc agattcacat
    17941 ataagattca acatcttgta tgcagtcttt ttagaaacac caagttccat aatgtctcta
    18001 tatccaagca acactttcat tcctttacac atcttttccc aacttcaaat ccacacatat
    18061 aaattgtttg aagcattgaa gatacgttta ataaatcttc attagaacat ccgttcttga
    18121 ttagaagatc taatactttt ccttccaaat gcactgagtc ttggaataat ctaattgaat
    18181 ctaatctgtc actttctaat cttccacttg gttctgccat tttattcacc ccttctataa
    18241 ttacggtatt accgtagtta aaacttaaaa aaattaaatt ttaactatat ctaatgatac
    18301 gtttgcttta ctacaaattt ttttaacttc ccaaaagtag aaatttttaa ttccactttc
    18361 ttttctttgg tagctaacag tagatatacc aagatattta gccatctctt tttgagtcat
    18421 atttaatccg actcggattt gtttgattgt taattccata ttaagcacct cctgactacg
    18481 gtgtcacctt attacactta taatatacgg cagtaccgta gttatgtcaa tcattttttt
    18541 atttttttac gcttatacct tagtttttta tttattttat gctatattta ttatagtaga
    18601 ttaaatttgt tattaattta agaaatcgag gtgacatata tgggtaacaa atttgaaagc
    18661 caagggcaag tattaaaaaa atttagaaag gagtgtggct ttacaaatca agaagttgta
    18721 gatagagtag gcaaatcgcc tatgtggcta tctgatattg aaaatggaaa atcgaatctt
    18781 tttttcaaag atgctaaaaa gttatgccat atatacggcc atactttag
//
LOCUS       DBMDWL010000019        18113 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1048_length_18113_cov_2.686487, whole genome shotgun sequence.
ACCESSION   DBMDWL010000019 DBMDWL010000000
VERSION     DBMDWL010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 18113)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18113)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18113
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_18113_cov_2.686487"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="ACZPDN_02440"
     CDS             <1..621
                     /locus_tag="ACZPDN_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297512.1"
                     /translation="DMLEEASYMNFKNELDACSVILANDESTQDEINDSLARLNAYFE
                     NKDNFVYKVMTLENNVVVDPQKQMLDTPQLVVQEQVNTVQSVQTPSPKATTSAADNFI
                     KTYLTSASGNVFTTANYLNYQKILSAMPSWLKLSSPDQKAVNTELKNKVGKTYQKLLQ
                     EAQNLKMNSGTSINTATNTDSTLYTWLCVMSLGMLTFVTKRLRKQD"
     gene            687..2195
                     /locus_tag="ACZPDN_02445"
     CDS             687..2195
                     /locus_tag="ACZPDN_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1297513.1"
                     /translation="MKQQKYNVKDLTLAYFQKKSKLYRAGGYKYATPLKRSLSDYQDH
                     LFAFLMDINICLLPVYIWVIEFLLIMCGLIPPHFFDLLFYIMFALLFVSSVLLLAFFT
                     ARTNGQSFGYAMLDLKLVRKKDKKEAMPLNLILRQALGFGVPLMIFGFFFQVLGVILW
                     WIINGIFVLVMPHQQTLFDLIFGLVPVREPDQEIRFETKPEPVKEELHVTPIDLHIRS
                     NYSDDGYYDVEELFKQAKDNGLEVISITDHNCARANAAAMRFSSLYNIQYIPGVEIDA
                     QYKRMRVRILGYYIDWTNEVFEVLEQNSLKREKELSIERVEKFENFSGIRIDVDSLMS
                     NSRFQTITPTEITKMVFHNERTRSFPFVKKYLDNCGSHSAAMSRFKTDVFGKNGPCYV
                     KADYPDAKAVIDAIHNAGGIAILSSWHLDYISDEVLEEIVDLGMDGVECFSNDIHEQT
                     IAAALKIVQKRKLFVSCGSDYHGPTKPKYHMGVSNCPEKALALVRILTKAAK"
     misc_binding    2236..2433
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2473..4398
                     /gene="metG"
                     /locus_tag="ACZPDN_02450"
     CDS             2473..4398
                     /gene="metG"
                     /locus_tag="ACZPDN_02450"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR1297514.1"
                     /translation="MTQKKEPFYLTTAIAYTSGRPHIGNTYEIILSDAIARFKRAQGY
                     DVYFQTGTDEHGVKIEEKAKAAGVTPQEFVDGVAAQIKSNWDLMNTSYDYFVRTTDDY
                     HVKEVQSIFKRLYDQGDIYKGTYEGWYCTPCESFWTESQLVDGCCPDCGRPVKKAKEE
                     AYFFNMQKYADRLIKYIEDHPDFIQPESRKNEMLNNFLRPGLQDLCVSRTSFSWGVPV
                     DFDPKHVVYVWIDALSNYITTLGYHANGESDEKFKKYWPATHIIGKDILRFHTIYWPI
                     ILMALDLPLPKKVFGHPWLLTGSDKMSKSKGNVIYAEDLVEHFGVDAVRYYCLHEMPF
                     AQDGTITWDLVIERINSDLANILGNLVSRTIAMSNKYFSGLVTNPNVCEAVDEELKAC
                     ALETKKKVEAKMEDLRVGDALDEVFTLLRRTNKYIDETMPWVLAKDESKQDRLATVLY
                     NLLESIRISAVLLHSFLPETAEKMFAYLNTKVTDLDSCDSFGNLETDIHVVEKCEPLF
                     ARIDEKKFMEEFNKKKEETKKEEEKVEEVTIDDFAKLQFKVGTIVKCEPHPKADRLLV
                     EQVDLGGEVRQIVSGIAKHYKPEELIGKQVVVVTNLKPVKLRGVESYGMILCATDDKD
                     LSFVTVAKEMPNGVTVR"
     gene            4398..4580
                     /locus_tag="ACZPDN_02455"
     CDS             4398..4580
                     /locus_tag="ACZPDN_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297515.1"
                     /translation="MNDKQKVVFKQLQLAGLGVVVFVFGLLYQEKAISMVGIGILIFG
                     LLRTYYIKKVIDKLDE"
     gene            complement(4638..5798)
                     /locus_tag="ACZPDN_02460"
     CDS             complement(4638..5798)
                     /locus_tag="ACZPDN_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1297516.1"
                     /translation="MKVFNHKETEFIMINIPEILVANGTGAFLVLFLLLYRIRISQTN
                     QFDEKAYNFMLIVTFIATINETLSFIIDARPGFIFHILQYISNTISSASSGIIGYCWC
                     LFVEYHIHRNFKRIKKKSRILAVPLIIATILIFINLLGTGIIFDISKENVYTRGPMNF
                     ILYIFVFVYYIESIYTVQKAKNDSILVEFFPIYFFIIPCMIGTMIQGFFFGISTIWLC
                     VAIAFIIVYIEIQISISFIDDLSGLYNRKYMNHYLDKLQNDKPKRVYGFLMDINDFKT
                     INDIYGHLKGDQAIIQFGKILQHSIDKDSVAIRMGGDEFVIFAILKSNEEALALKKHI
                     EQNVRQFNTNSTEPFHLSFSIGIAKYSGNIETFLSSMDDSMYEAKNMHRLMH"
     gene            5980..6156
                     /locus_tag="ACZPDN_02465"
     CDS             5980..6156
                     /locus_tag="ACZPDN_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297517.1"
                     /translation="MTMIKNSVVELFILLLLVCTVFIYKLPSTMNTWLLNSIVIVVMG
                     VCILRIALKMMNHK"
     gene            6188..6484
                     /locus_tag="ACZPDN_02470"
     CDS             6188..6484
                     /locus_tag="ACZPDN_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297518.1"
                     /translation="MWYIIGMKKFLKDIAQGVYDGLQENEGTHNQYSFQDIYDVIQEF
                     VSYIFVGMILSVGAIFAYPYYKAFISLGAVLLLVWAFILYYFLNSTDFKKIKKK"
     gene            6494..7270
                     /locus_tag="ACZPDN_02475"
     CDS             6494..7270
                     /locus_tag="ACZPDN_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1297519.1"
                     /translation="MQIVLVLLVAFVAGIEGILDEFEFHQPIVACTLIGLVTNHLAEG
                     LLLGGSLQLIALGWANIGAAVAPDVCLASVISSILMIHGLESAIEARFVIAFSIALAI
                     PLSLVGLSLTKVCRNLAIQLVHKMDEAKKVSYYQWIGICMQGIRVLIPTIVLLLIPSS
                     MICSILEMIPVSIYKGLLIGSGVVCVVGFAIVANVLGSKFTYPFFMIGFGLACMTNLS
                     LISLCFIGGGLVWLYMLLNAKEKSNKTSDDPLGDILNDYE"
     gene            7280..8044
                     /locus_tag="ACZPDN_02480"
     CDS             7280..8044
                     /locus_tag="ACZPDN_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1297520.1"
                     /translation="MKKSTRLKVCLNHQYLQGSWNFERMQNGGWCYGLLPALKELYPN
                     KEDFDKAIDRHLKFYNTHPYVSAPIVGIVLAMEEEKAKGADISDTSIQDVKIGLMGPL
                     AGVGDPVFWFTMRPILAAVGASLALSQNVLGPIFFFVAWNVLRFGFLWITQELGYKKG
                     TKLITDLSDTFLKNVTLAASMLGMFVVGILVQRFVSIDVVKIQSDLDMVLPGVLSLCL
                     FFGCTKILKKGFSPVLLIVILFILCVILEMAGILVI"
     gene            8173..8703
                     /locus_tag="ACZPDN_02485"
     CDS             8173..8703
                     /locus_tag="ACZPDN_02485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1297521.1"
                     /translation="MSRQILKKEHIITDRLTLGPYRKEDRERLKEMMQNPKITATFMV
                     PDYCEEVQFYALADKLIEFSQINDTIHLEYGIYLDGYMIGFVNDCGYDDEAIELGYVI
                     DPSFQGRGFATEAVNAVINELREMGFKKVVADFFEENIGSRKVMEKCGMHLNGNSDYA
                     EYRGKKFKCYECEMEL"
     misc_feature    8866..8936
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            8966..9268
                     /gene="rplU"
                     /locus_tag="ACZPDN_02490"
     CDS             8966..9268
                     /gene="rplU"
                     /locus_tag="ACZPDN_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1297522.1"
                     /translation="MYAIIETGGKQIRVEKDAVIFVEKLEANEGDTVTFDKVLYADGK
                     FGKPFVEGAKVTGVVEKQGKGKKITIVKYKAKKSSTRRKQGHRQPYTRVKIEAIEA"
     gene            9271..9585
                     /locus_tag="ACZPDN_02495"
     CDS             9271..9585
                     /locus_tag="ACZPDN_02495"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016233.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR1297523.1"
                     /translation="MIKIKIARDDNGISLISMHGHAEYDQYGKDIVCSAASTIIIGGI
                     NAIAQLGYLSFISYDVDAGDVELKINNTECAELQIILNTLLVQFQSIEESYPKNIQVQ
                     EV"
     gene            9589..9873
                     /gene="rpmA"
                     /locus_tag="ACZPDN_02500"
     CDS             9589..9873
                     /gene="rpmA"
                     /locus_tag="ACZPDN_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1297524.1"
                     /translation="MLLKLDLQLFASKKGVGSTKNGRDSHSKRLGAKLADGQSCHAGS
                     IIYRQRGTKIHPGTNVGRGADDTLFALVNGVVKYERLGKDKKKVSVYPQA"
     gene            9938..11218
                     /gene="obgE"
                     /locus_tag="ACZPDN_02505"
     CDS             9938..11218
                     /gene="obgE"
                     /locus_tag="ACZPDN_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR1297525.1"
                     /translation="MFVDQVKVHIKAGKGGDGLVSFRHEKYVAYGGPFGGDGGNGGDV
                     IFEADPGMTTLLDLRYHRKIIATPGEKGKNKKMHGANGEHKVVKVPLGTIVKRSDNNQ
                     VLADLTKPHQRQIVAHGGRGGRGNWHFRSSHNTAPKYAEQGVLGEEFDCVVELRVLAD
                     VGLVGFPSVGKSTFLDAVSKARPEIGDYPFTTITPNVGVVQTGDGRSFVLADLPGLIE
                     GASDGKGLGHQFLRHIERCRVIIHVIDMGAEDGRDPLKDYEVINNELKSYQIRLLERP
                     QIVVANKMDMENAQENVRRFKEKYPDIPVYETTTIIHEGLDAVLRKAADLLATTPAFP
                     ITEDTGETGVMYTFEEKKKDILIEKEDDGVWNVSGPRLERVFDINKLNTEEDFYLFAN
                     KMRYLGIDTALREAGCQDGDTVRLLGFEFEFIEN"
     gene            11279..12028
                     /locus_tag="ACZPDN_02510"
     CDS             11279..12028
                     /locus_tag="ACZPDN_02510"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1297526.1"
                     /translation="MKCISWNVNGIRAVLKKDFVEILYALDADIFCIQETKAQPDQIE
                     VDCELYPYQYINSAQKKGYSGTMIFCKEEPLSIRYGMNMEEHDTEGRVITLEYPEYYV
                     VTVYTPNSGDGLKRLDYRMEWDKAFSAYLKSLDKPVMACGDFNVANEEIDIKNPKTNH
                     KNAGFTDEERDSFKENLLAYFVDTYRSLYPEKVEYSWWSYRFKAREKNAGWRIDYWLV
                     SPELKDKIVDAKILTDIYGSDHCPVQLDIEV"
     gene            12030..12608
                     /gene="ruvA"
                     /locus_tag="ACZPDN_02515"
     CDS             12030..12608
                     /gene="ruvA"
                     /locus_tag="ACZPDN_02515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1297527.1"
                     /translation="MIAFVNGIVKIIRNDRVVLDVHGVGYEVYLANALSQKMNEELFL
                     YTYQHVREDAILLFGFIKEEDYEVFMRLINVKGIGPKTAQTMLSVCSGKEMIEAIEND
                     DIKRLKSLPGIGAKTAGQIVLDLKGKFVSLETSDSPVSNPVWTQVQDALLSLGYKTNQ
                     LTKIKKEFENTELGEDEMLRQALILLAKRNGV"
     gene            12614..13624
                     /gene="ruvB"
                     /locus_tag="ACZPDN_02520"
     CDS             12614..13624
                     /gene="ruvB"
                     /locus_tag="ACZPDN_02520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1297528.1"
                     /translation="MPREVDGNLQECDNFEKTLRPQSLNEYIGQSELKENLNVFIHAA
                     RSRNEALDHVLFYGPPGLGKTTLAYILANEMHGNLKTASGPSIEKSGDLAAILTTLEP
                     GDVLFIDEIHRLPKQVEEVLYPAMEDYCLDIVVGKDSATVHNIRINLPPFTLVGATTR
                     AGDLSAPLRDRFGIISQLEYYNLQDLEKIVYRTSCVMNTEIDKNAVAEIALRSRGTPR
                     IANRLFRRVRDFAQVYNEGVISSEIAKTALDRLRVDHLGLDAVDHKYLKGIIERFKGG
                     PVGLEAIASSIGEEPMTLEDVYEPYLLQIGFINRTPRGRIVTPKAYEHLQIPYQKELF
                     TD"
     gene            13665..15218
                     /locus_tag="ACZPDN_02525"
     CDS             13665..15218
                     /locus_tag="ACZPDN_02525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1297529.1"
                     /translation="MEQMTELTKKVLEFLEPDSSKWAYWTSKQLLNPVSESKCMESFK
                     ERILKAVSNREKVIVAGDYDCDGISATTIMVSGLRKLGLECGFYIPDRIKEGYGLSEA
                     TVMLAHKKGYSLILTVDNGIKSKPALALSKELGMDVIVTDHHTMDEEVNCDIVVHPDL
                     MEPCFETLCGAGIAYECMRKLGVEDDYLLQLAGLASISDMMVVKGQTRALIQNALRSI
                     NETHEKHIFSLATDRDLNETSIGFQVVPKLNAIGRLSNLANVNNVVRYFLAHDDETIY
                     TLGSQITQMNTIRKQMSDQMQKTALLKCKSNEDIYIIEDTSFHEGIIGLVAGALCSRF
                     NKPCIVLAKNEQGYKASMRSPEGFNCMEFLGPYKHFVVFGGHENAAGFSLNLNEFDLF
                     EVFVHARIKEYTYSVKKKETLIITDDELSLETIQSLDVLRPFGPGFVFPSFEIIHPQI
                     KSLYDFQNHKHRKYTLESGLQCMRFNQSEVEYKKSVNAIHSFIGTAQINQYQGRKQVN
                     FVIDEIVYE"
     gene            15250..15771
                     /locus_tag="ACZPDN_02530"
     CDS             15250..15771
                     /locus_tag="ACZPDN_02530"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR1297530.1"
                     /translation="MDLKDYIESIPGFPKEGIIFRDVTPILQNASAMRYCVDQLNDFV
                     KSVGADVVIGPEARGFLFGVPVALENNVGFVPVRKPGKLPRETVSETYDLEYGQDELC
                     VHADAILPGQKVVIIDDLLATGGTVEAIIKMVERMQASVTGVAFVIELDDLKGREKFK
                     DIPVCALTHYEGE"
     gene            15806..18010
                     /locus_tag="ACZPDN_02535"
     CDS             15806..18010
                     /locus_tag="ACZPDN_02535"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1297531.1"
                     /translation="MSQKKQVTFEECMENVQTYIKRPENIELIQKAYDFAYEHHKGQF
                     RKSGEPYVIHVIQVANTLALMHCGPKTIAAGLLHDTVEDCQGVTTDTITELFGQEIAT
                     LVDAVTKIGAIKFKDEEEYLASNHRKLFIAMAKDIRVILIKLADRLHNMRTLQYMRPE
                     KQKKIARETLSVYAPIAHRLGISEIKNELEDLSFKYLDYKKYEEIKDLVKQRESDRNE
                     QVHEMISDIESIMQEYNIQYRIFGRSKHFYSIYKKMVTKNKRFEEILDLLAIRIVTDS
                     VVHCYEILGYIHAKYRPIPGRFKDYIAMPKANMYQSLHTTIVEPEHGNIFEIQIRTEE
                     MDAIAERGVAAHWKYKENRNYTPEYEQKEIEDKLSWFRDFSMMTDEESEDPLEYMNVL
                     QKDIFEANVYCLTPRGKVIALPSGSCPIDFAYRIHTEVGNKTVGAKVNGAIVPLNTPL
                     KTGDVVDILTNNNSVGPSADWIKIVKSGHARNKIRTFLQKQEQQSRKESIKLGQSMLE
                     DEFRRLKLDPKSMDQKRLESILTSLSFKSVDDLYVGIAMKRVSLQSIVDRLVKNRSNM
                     LEDQEIMKIYNKQPAHVTKHSSCGVIVPGVDTIAVSLANCCRPIPGDSIIGYISKGQG
                     VKVHRADCPNIVNEKKRLIPVQWEEGLDEKQYEVNLIIHSDDRNYLLSDIVTTLQQCN
                     VYLKHVDSAVDDGNLEATTKLTVSVKNAAHLQNLISNLKKVRSVNEVIRTIQ"
BASE COUNT         6091 a         2480 c         3801 g         5741 t
ORIGIN      
        1 gatatgcttg aagaagcatc ttatatgaat tttaaaaatg aattggatgc ttgtagtgtt
       61 attcttgcaa acgatgaatc cacacaagat gaaatcaatg attcattagc acgattgaat
      121 gcttattttg aaaataaaga taattttgta tataaggtga tgacactaga aaataatgtg
      181 gttgtagatc ctcaaaaaca aatgttggat acacctcaat tggttgtgca agaacaagtg
      241 aatacagttc aatctgtaca aacaccttcg cctaaggcaa caacaagtgc tgcagataat
      301 ttcattaaga cttatttaac gagcgcgagt gggaatgtct ttactaccgc aaattattta
      361 aactatcaaa agattctttc ggcaatgcct agctggttaa agttatcgtc accggatcaa
      421 aaagcagtca atacagaatt aaaaaataag gttggtaaaa cttaccaaaa attattacag
      481 gaagcacaga atttaaaaat gaattctggt acaagtatca acactgctac aaatacagat
      541 tctacattat atacatggtt atgtgtaatg tctttgggta tgttaacatt tgtaacaaaa
      601 cgtctaagaa aacaagatta agggccgggg cccttttttt catgtttaaa aattgttata
      661 atagtgcgag aaaggcttat gattttatga aacaacaaaa atacaatgtt aaggatctaa
      721 ctctagcata ttttcaaaaa aaatcgaaat tgtatcgtgc aggtggatat aaatatgcga
      781 cgcctttaaa acgtagtcta tccgattatc aagatcattt atttgcgttt ttaatggata
      841 tcaatatttg tttacttcct gtttatatat gggttattga gtttttatta attatgtgtg
      901 gattgattcc accacacttt tttgatttat tattctatat tatgttcgca ctattatttg
      961 tgtcgagcgt cttattgctt gcatttttta cagctcgaac aaatggtcaa tcttttggct
     1021 atgccatgct ggatttgaag ttagtcagaa aaaaagataa aaaagaggca atgccattaa
     1081 acttgatttt aagacaagca ttaggctttg gtgttccact tatgattttc ggcttcttct
     1141 ttcaagtttt aggtgttatt ttatggtgga taatcaatgg aatctttgta ttggttatgc
     1201 cacaccaaca aactttattt gatttgatat tcggcttagt tccagttcgt gaaccagatc
     1261 aagagattcg ttttgaaaca aagccagaac cggttaaaga agaattacat gttacaccaa
     1321 tcgatttaca cattcgatca aattatagtg atgatggata ttatgatgtt gaagaattgt
     1381 tcaagcaggc aaaggataat ggtttagaag tgatttcgat tacggaccat aactgtgcaa
     1441 gagcaaatgc ggcggctatg cgtttttcta gcctgtataa tattcaatat attccaggcg
     1501 ttgaaattga tgcacaatat aaaagaatgc gagttcgtat tctaggctat tatattgatt
     1561 ggacaaatga agtgtttgaa gtactcgaac aaaattcttt aaagcgtgaa aaagaattat
     1621 cgattgaacg tgtggaaaaa tttgaaaatt tctcaggaat tcgtattgat gtggattctt
     1681 tgatgtcaaa ttctcgtttt caaacaatta cgccaacgga aatcacaaaa atggttttcc
     1741 ataatgaaag aacgcgttca tttccatttg tgaagaaata tttggataat tgtggaagcc
     1801 atagtgctgc aatgtcaaga tttaaaacag atgtatttgg caagaatgga ccttgttatg
     1861 taaaggcaga ttatccagat gctaaagcag tcattgatgc gattcataat gctggtggaa
     1921 ttgcgatttt atcaagctgg catttagatt atatttccga tgaagtgtta gaagaaattg
     1981 tcgatttagg tatggatgga gtggaatgct tctcaaatga tattcatgaa caaacgattg
     2041 cggcagcttt aaaaattgta cagaaacgta aattgtttgt ttcttgtggt agtgattatc
     2101 atggtccgac taagccaaaa tatcatatgg gtgtttcaaa ttgcccagaa aaggcattgg
     2161 cacttgtaag aattttgaca aaagccgcaa aataaatttg ctcaaaatat gttttatagc
     2221 gtataataaa gcagtcaata agcaaagaaa agaagagtat ttatattgca tactagagag
     2281 agcagatgtt tggtgaaaat ctgtgtataa gatataaaga atatggtctt ggagcttctt
     2341 tatcgatatg tagataaaga cgtatgctgg cgttaacagc ttgaggtgtg caaacacaaa
     2401 ctaaggtggt accacgaagc tttcgtcctt ggatgaaggc tttttttatt tgtaaaatag
     2461 gaggaaatag aaatgacaca aaagaaagaa ccgttttatt taacaactgc cattgcgtat
     2521 acatcaggta gacctcatat tggaaataca tatgaaatta tcttatcgga tgcgattgct
     2581 cgttttaaaa gagcacaagg atatgatgta tatttccaaa caggtacaga tgagcatggt
     2641 gtaaaaattg aggaaaaggc aaaagctgca ggagttactc cacaagaatt tgtcgatggt
     2701 gtagctgctc aaatcaaatc caactgggat ttaatgaata caagctatga ttattttgta
     2761 cgtactacag atgattatca tgttaaagaa gtacaaagta tttttaaacg tttatatgac
     2821 caaggagata tttataaagg aacatatgaa ggatggtatt gtacgccttg tgaatctttc
     2881 tggacagaat ctcaacttgt tgatggatgt tgcccagact gtggtcgtcc agtaaaaaag
     2941 gcaaaagaag aagcttactt cttcaatatg caaaaatatg cagatcgttt gattaaatat
     3001 attgaagatc atcctgattt tattcaacca gaatctcgta aaaatgaaat gttgaacaac
     3061 ttcttacgtc caggattaca agatctatgt gtatctcgta cttcatttag ttggggagtt
     3121 cctgttgatt ttgatccaaa acacgttgta tatgtttgga tcgatgcatt aagtaactat
     3181 attacgactc ttggatatca tgccaatggt gaaagtgatg aaaaatttaa aaagtactgg
     3241 cctgcaactc atattattgg taaagatatt ttgcgtttcc acacaatcta ttggccaatt
     3301 atcttaatgg ctttagattt gcctttgcca aagaaagtat ttggacaccc atggctactt
     3361 actggtagtg ataaaatgtc taaatcaaaa ggaaatgtaa tttatgcaga agatttagtg
     3421 gaacactttg gtgtagatgc cgtacgttat tattgtttac atgaaatgcc atttgcccaa
     3481 gatggaacaa ttacttggga tctagtgatt gaaagaatca attcggactt ggcaaacatt
     3541 ttaggtaact tagtatctcg tacaattgca atgtcaaaca aatatttctc tggtttagtc
     3601 acaaatccaa atgtatgtga ggcagtcgat gaagaattga aggcttgtgc tttagagact
     3661 aagaaaaaag tagaagctaa aatggaagat cttcgtgttg gtgatgcatt agatgaagta
     3721 ttcacattgc ttcgtcgtac aaataagtac attgatgaaa caatgccttg ggtacttgct
     3781 aaagatgaat cgaaacaaga tcgtttggca acagttcttt ataacttgct tgaatcaatt
     3841 cgtatttcag ctgtattatt acactcattc ttgcctgaaa cagctgaaaa gatgtttgcg
     3901 tacttaaata caaaagtaac cgatttagat tcttgtgatt cttttggaaa cttagaaaca
     3961 gatattcatg tcgttgaaaa gtgtgaacca ttgtttgctc gtattgacga aaagaaattt
     4021 atggaagaat tcaataagaa aaaagaagaa acgaaaaagg aagaagaaaa agtagaagaa
     4081 gttacaattg atgattttgc gaaactacaa tttaaagtag gtacgattgt aaaatgtgaa
     4141 cctcatccaa aggctgatcg tttgttggta gaacaagttg atttgggtgg tgaagttcgt
     4201 caaattgtga gtggtatcgc aaaacactac aaaccagaag agttgattgg aaaacaggtt
     4261 gtcgtagtga caaacttaaa accagttaaa ttacgtggtg tagaatctta tggtatgatt
     4321 ctttgtgcta cagacgataa agacttatca tttgtcacag tcgcaaaaga aatgcctaat
     4381 ggtgtaactg ttcgctaatg aatgataaac aaaaggttgt ttttaaacaa ttacaacttg
     4441 ctggacttgg cgttgtcgtc tttgtgtttg gtttgttgta tcaagaaaaa gccatttcta
     4501 tggttggaat cggtatcttg atttttggtt tattaagaac ctactacatt aaaaaagtaa
     4561 ttgataaact agatgagtaa tcaataaatt gctcatcttt ttttgtgcaa attaaaaagg
     4621 cgatgaactc gccttgatta gtgcattaaa cgatgcatgt ttttagcttc atacatggaa
     4681 tcatccatcg atgataagaa tgtctcaata ttaccgctgt atttagcaat tccaattgag
     4741 aaagataagt ggaatggttc tgttgaattc gtgttaaatt gtcgtacgtt ttgttcaata
     4801 tgtttcttta gggctaaagc ttcttcattg gattttaata ttgcgaagat cacaaattca
     4861 tctccaccca tacgaatggc aactgaatct ttatcgattg aatgttgtag aatttttcca
     4921 aactgaatga tggcttgatc cccttttaaa tgtccatata tatcgttgat cgttttaaaa
     4981 tcattaatat ccatcaaaaa tccatataca cgttttggtt tatcattttg tagcttatcc
     5041 agataatggt tcatgtattt tcgattgtaa agtccggata aatcatcaat aaaggatata
     5101 gatatttgaa tttcaatgta aacgatgata aatgcgattg cgacacaaag ccaaatcgta
     5161 gaaattccaa agaagaaacc ttgaatcata gtgccaatca tgcaagggat gataaagaag
     5221 taaatgggaa agaattcgac taaaatggaa tcgtttttag ctttttgaac agtatatata
     5281 ctttcaatat aataaacgaa gacgaaaata tataaaataa aattcatggg cccacgagta
     5341 tatacatttt cctttgaaat atcaaagatg atacccgttc ccaataaatt gataaagatc
     5401 aagattgtgg caatgatcaa tggaacggct aaaattcttg atttcttttt aatgcgttta
     5461 aaatttctat gaatatgata ttctacgaat aaacaccaac aatatccgat gatgcctgag
     5521 ctagctgaag aaattgtatt tgagatatat tgtaaaatat gaaatataaa acctgggcgt
     5581 gcatcaatga taaaactaag cgtttcattg atcgtggcaa taaaggtaac tataagcata
     5641 aagttatatg ctttttcatc aaactgattt gtttgggaaa ttcgtattcg atataacaat
     5701 aagaagagca ctaaaaatgc cccagtcccg tttgccacca gtatctcggg tatgttgatc
     5761 ataataaact ccgtttcttt atggttaaac actttcataa taatcctctt tataagaact
     5821 tgcaaattta aaaaggggaa tttgtagatc gtaatgtaaa ttaatcttta caaagattaa
     5881 gcataaatta agtaaatgct aaatgttagt tcaatcgtat cgagtcatat tgtactttgt
     5941 ttaaagcaat ctatactaaa agtataagga ggtacagata tgacgatgat taaaaattca
     6001 gtggtagaac tctttatttt acttctcttg gtatgtaccg tttttattta taaactgccg
     6061 agtacaatga acacttggtt attgaattca attgtgattg tggtaatggg cgtttgtatt
     6121 ttaagaatcg cacttaaaat gatgaatcat aaataatgtt ggcttttcct aaggggaaag
     6181 ccttttcttg tggtatatta taggtatgaa aaagttttta aaagatattg cacaaggcgt
     6241 atatgatgga ctacaagaaa atgaaggaac acataatcaa tatagttttc aagatattta
     6301 tgatgtgatt caagaatttg tttcctatat ctttgttggc atgattctaa gtgtaggagc
     6361 tatttttgcc tatccttact acaaagcatt tatatcatta ggtgctgtac tccttcttgt
     6421 atgggcattt atcttgtatt attttttaaa ttcaacggat tttaaaaaaa tcaaaaagaa
     6481 atgaggaaga gttatgcaga ttgttttagt gttgcttgtg gcatttgtcg caggtattga
     6541 aggcatttta gatgagtttg aatttcatca acccattgtt gcctgtactt taattggttt
     6601 agtgacaaac catttagcag aaggattatt attaggtgga tctttacaat tgattgcctt
     6661 aggatgggcg aatattggcg ctgctgttgc tccggatgta tgtttggcaa gtgtgatttc
     6721 atctattctg atgattcatg gccttgaaag tgcaattgaa gctagattcg taatcgcatt
     6781 tagtattgcc ctagcgatac cattatcttt agtgggttta tctttgacaa aggtgtgtcg
     6841 taatttagca atccaacttg tacataagat ggatgaggca aagaaagttt cttattatca
     6901 atggattggt atttgtatgc agggaattcg tgttttgatt cccacaatcg ttttattatt
     6961 gattccatca tccatgattt gttcaatctt ggaaatgatt cctgtttcaa tttataaggg
     7021 actattgatt ggttcgggtg tagtttgtgt ggttggcttt gcgattgtag cgaatgtatt
     7081 aggatctaaa tttacatatc cattttttat gattggattt ggacttgcat gtatgactaa
     7141 cttatcttta atatctttat gctttattgg tggtggctta gtttggttat atatgttgtt
     7201 gaatgcgaaa gaaaaatcga ataagacaag tgatgatccg ttaggggaca tcttaaatga
     7261 ttatgaatag gtggtttata tgaagaaaag tacaagatta aaggtttgtt taaaccatca
     7321 atatttacaa ggctcatgga actttgagcg tatgcaaaat ggtggatggt gctatggttt
     7381 acttccagca ttgaaagaat tatatccaaa caaagaagat tttgataagg ctatagatcg
     7441 tcatttaaaa ttctataata cacatccata tgtttctgca ccaattgttg gtatcgtttt
     7501 ggctatggaa gaagaaaagg caaaaggtgc tgatatttca gatacatcta ttcaagacgt
     7561 aaaaattggc ttaatggggc ctttagcagg cgtaggagat cctgtatttt ggtttacaat
     7621 gcgtccgatt cttgctgctg ttggtgcatc gcttgcgtta tcacaaaatg ttctaggacc
     7681 tattttcttc tttgtggcat ggaacgtact tcgttttggt tttctttgga ttacacaaga
     7741 gcttggttat aagaaaggta ctaagttgat tacggactta tcggatacat tcttaaagaa
     7801 tgtaacactc gctgcaagta tgttagggat gtttgtggtt ggaattttag tgcagagatt
     7861 tgtttcaatc gatgtagtaa agattcaatc ggatttagat atggtattgc caggagttct
     7921 ttctttatgt ttattttttg gttgtacaaa gatactaaaa aaaggctttt cacccgtatt
     7981 attaattgtc attttattta ttctttgtgt cattttagag atggcaggaa tcttagtgat
     8041 ttaagtctaa aaatatgatt tgtatacttc gtacaataat attatttaaa aagaaaaaga
     8101 taaatcagaa ttagtcacaa taaaaagcgt aattatcaat attgtatatc agcaagaagg
     8161 ggaaacagag tggtgagcag acaaatatta aaaaaagaac atatcattac agatagattg
     8221 acgttagggc catatcgtaa agaagataga gagcgtctca aagaaatgat gcaaaatcca
     8281 aagattacgg caacgtttat ggtgcctgat tattgtgaag aagtacagtt ttatgcgtta
     8341 gctgacaaat tgatcgaatt cagtcagatt aatgatacaa ttcatctaga atatggcatt
     8401 tatctggacg gttatatgat tgggtttgtc aatgattgtg gttatgatga tgaagctatt
     8461 gaattgggat atgtcattga cccatctttt caagggcgag gatttgcaac ggaagcagtg
     8521 aatgcagtaa taaacgaatt acgtgagatg ggctttaaaa aggtcgtggc ggactttttt
     8581 gaagaaaata taggcagtag aaaagtgatg gaaaagtgtg gcatgcatct aaacggaaac
     8641 tcagattatg cggaataccg cggaaagaaa tttaagtgct atgagtgtga aatggagctc
     8701 taaagaatta gcgagttcct aagcacattt ggtaatatat gttggtaaga agcaaagtaa
     8761 gataaattct agtttatcga tataaaacat agagctaaaa aatagaattt tatcttgacg
     8821 tattgcctta catcctttaa aatatcaatg ttgcaaactc cgtgtatgga cttgcaaccg
     8881 cccagaaaag gtttaagttg aaaattcaca ccttgatggc gagtatcaag ctaaatgtga
     8941 aatagaatga ataggaggtg cacacatgta cgcaattatc gaaactggtg gaaaacagat
     9001 ccgtgttgaa aaggatgctg ttatcttcgt tgaaaaatta gaagctaacg aaggagacac
     9061 tgttacattt gacaaagtat tatacgctga tggaaaattt ggtaaaccat tcgtagaagg
     9121 tgcaaaggta acaggtgttg ttgaaaaaca aggcaaagga aagaaaatca ctatcgtgaa
     9181 atacaaagct aaaaaatctt caacacgtcg taaacaagga caccgtcaac cttacactcg
     9241 tgtaaaaatt gaagctatcg aagcttaatt atgattaaaa ttaaaattgc tagagatgat
     9301 aatgggattt cattgatctc tatgcatgga catgctgaat atgatcagta tggaaaagat
     9361 atcgtctgtt ctgcagccag taccattatt attggtggta tcaacgcaat cgcacagctt
     9421 ggctatttaa gtttcatttc ttatgatgta gatgcagggg atgttgaatt gaaaataaac
     9481 aatacagaat gtgctgaact acaaatcata ctaaatacgc tattagttca gtttcaaagt
     9541 attgaagaaa gttatcctaa aaatattcaa gtacaggagg tgtaaaaaat gttattaaaa
     9601 ttagacttac agttattcgc ttctaaaaag ggagttggtt ctacaaagaa cggtcgtgat
     9661 tctcactcaa aacgtttagg agctaaatta gctgatggac aatcttgtca cgctggaagc
     9721 attatttatc gtcaacgtgg aacaaaaatt catccaggaa ctaacgtagg acgtggtgct
     9781 gatgatacat tgttcgcatt agttaacggt gttgttaaat acgaacgtct tggtaaagat
     9841 aaaaagaaag tttctgttta tccacaagct taacaagtgt gaaatttaag atcccgttgc
     9901 tgggatcttt tttaatgaaa tggaaaggag ggtatatatg tttgtagatc aagtcaaagt
     9961 tcatattaaa gcaggtaaag gcggagatgg tttagtaagt tttcgccacg aaaaatatgt
    10021 tgcctatggt ggacctttcg gaggcgatgg aggtaacgga ggagatgtca tttttgaagc
    10081 agatccaggt atgacaactc tattagattt gcgttatcat cgtaaaatta tcgcaactcc
    10141 gggtgaaaaa ggtaaaaata aaaaaatgca cggagccaat ggggaacata aagttgttaa
    10201 agttccatta ggaaccattg taaagagaag tgataataat caagtacttg ccgatttgac
    10261 aaaaccacac caacgccaaa ttgttgctca tggaggtcgt ggaggtcgtg gaaactggca
    10321 ctttagatca agtcacaaca cagcacctaa atacgcagag caaggtgttt tgggtgaaga
    10381 atttgattgt gttgtagaat taagagtact tgccgatgtt ggattggtag gatttccaag
    10441 tgtaggaaaa tcaacattcc tagatgccgt aagtaaagct cgtcctgaaa ttggagacta
    10501 tccatttacg acgattacac caaatgttgg ggttgttcaa acgggtgatg gaagaagttt
    10561 tgttttagcc gatttaccag gacttattga aggagcaagt gatggtaaag gtttaggtca
    10621 tcaattcttg cgccatattg aaagatgtcg tgttatcatc catgtgattg atatgggtgc
    10681 agaggatggc agagatccat taaaagatta tgaagttatt aataatgaat tgaaatctta
    10741 ccagattcgt ttattggaaa gacctcaaat tgttgttgcc aacaagatgg atatggaaaa
    10801 tgcccaagaa aatgttcgtc gttttaaaga aaagtatcca gatattccag tctatgaaac
    10861 aacaacaatt attcatgaag gtttagatgc agtacttaga aaagcggcag atcttcttgc
    10921 gacaacacct gcattcccaa ttacggaaga tacgggtgaa acaggtgtta tgtatacatt
    10981 tgaagagaag aagaaagaca tccttattga aaaagaagat gatggtgtat ggaatgtttc
    11041 aggtcctaga cttgaaagag tattcgacat caataaattg aatacggaag aagacttcta
    11101 tctatttgcc aacaaaatgc gttatcttgg aattgacaca gctttaagag aagcaggatg
    11161 tcaagatgga gacacagtac gtttattagg ctttgaattt gaatttattg aaaactaagg
    11221 ttacacattg tgtagccttt tcttttcgtg tggtataatg cttctgaggt gtttttttat
    11281 gaaatgtatt tcgtggaatg taaacgggat tcgtgccgtt ttaaagaagg attttgtgga
    11341 aattctttat gcgttggatg cagacatttt ttgtattcaa gaaactaagg cacaacccga
    11401 tcaaattgaa gtggattgtg aattatatcc ttatcaatat ataaatagtg ctcaaaagaa
    11461 agggtatagt ggaacaatga ttttctgcaa agaagaacca ttatccattc gttatgggat
    11521 gaatatggaa gaacatgata cagaaggaag agtgataact cttgagtatc ctgaatatta
    11581 tgttgtgact gtttatacac caaattcagg ggatggacta aaaagattag actatcgtat
    11641 ggaatgggat aaagctttta gtgcctatct aaaatcattg gataaaccgg ttatggcttg
    11701 tggcgatttt aatgtggcca atgaagagat tgatattaaa aatccaaaga caaatcataa
    11761 aaatgctgga tttacagatg aagaaagaga tagttttaaa gaaaatctat tagcttattt
    11821 tgtggataca tatcgtagtt tatatccgga aaaagtagaa tattcgtggt ggagctatcg
    11881 ttttaaggcg cgtgaaaaga atgcaggatg gcgaatcgat tattggctag tatcgccgga
    11941 attaaaagat aaaattgtgg atgcgaagat cttaacggat atatatggtt cagatcattg
    12001 tccagtccaa cttgatattg aggtgtaaaa tgattgcgtt tgtgaatgga attgtaaaga
    12061 ttattcgaaa tgatcgtgtt gtacttgatg tacatggcgt tggctatgaa gtgtatttgg
    12121 ctaatgcgtt aagtcaaaaa atgaatgaag aattatttct ttatacatat caacacgttc
    12181 gtgaagatgc gatcttattg tttggtttta tcaaagaaga agattatgaa gtctttatgc
    12241 gtttgatcaa cgtaaaagga attggtccaa aaacagccca gaccatgtta tcggtatgtt
    12301 ctggaaaaga aatgattgaa gccattgaaa atgatgatat taaaagattg aagtcattac
    12361 ctggtattgg tgctaaaacg gcaggacaaa tcgttttgga tctaaaaggt aagtttgtat
    12421 ctttagaaac gagtgatagt cctgtgtcaa atccagtatg gactcaagtt caggatgctc
    12481 ttttatcact tgggtataag accaatcaat taacaaaaat taagaaggaa tttgaaaata
    12541 cagaattagg agaagatgaa atgttacgac aagcattgat tctactcgca aaaagaaatg
    12601 gagtataaga taaatgccta gagaagtaga tggaaattta caagagtgtg ataactttga
    12661 aaagacgtta cgtccgcaaa gtttaaatga atatattggg caatctgaat taaaagaaaa
    12721 tctaaatgtg tttattcatg cggcaagatc tcgaaatgaa gctttagatc atgttctatt
    12781 ctatggccct cctggtcttg gaaaaacaac tttagcctat attcttgcca atgaaatgca
    12841 tgggaattta aagacggcaa gtggtccttc cattgaaaaa agtggagatc ttgcagctat
    12901 acttacaaca cttgaaccag gagatgtctt atttattgat gagatccatc gtttgccaaa
    12961 acaagtggaa gaagtattgt atccggctat ggaagattat tgtctagata ttgtggtggg
    13021 taaagattca gcaaccgtac acaatattcg tattaattta ccacctttta cgcttgtggg
    13081 agccacaact cgtgcaggag atttaagtgc gcctttgcga gatcgctttg gaattatttc
    13141 acagctagag tactataatt tacaagattt agaaaaaatc gtttatcgta caagttgtgt
    13201 gatgaataca gaaattgata aaaatgccgt tgcggagatt gctttaagat cgcgtggtac
    13261 accacgaatc gcaaaccgtt tgttcaggcg tgtacgtgat tttgcccaag tctataatga
    13321 gggtgtgatt tcaagtgaaa tcgcaaagac agctttagac aggcttcgtg tagaccattt
    13381 aggtttagat gcggttgacc ataaatattt aaaaggaatc attgaaagat ttaaaggcgg
    13441 gcctgtagga cttgaagcga ttgcttcaag tattggagaa gagccaatga ctttagaaga
    13501 tgtatatgag ccatatttat tacagattgg ttttatcaat cgtacgccta gaggaagaat
    13561 agtaacgccc aaagcgtatg aacatcttca aataccatat caaaaagaat tgttcacaga
    13621 ttaatagtct gtgaacattt ttattatatg gtataataat ttgcatggaa caaatgacag
    13681 aattaacaaa aaaagtatta gaatttttag aaccagattc atcgaaatgg gcgtattgga
    13741 caagtaaaca gcttttaaat ccagtttctg aatcgaaatg tatggaaagc tttaaagaac
    13801 gaatattaaa ggctgtttca aatcgtgaaa aagtaattgt agcaggagac tatgattgtg
    13861 atggtatcag tgcaacaaca atcatggtgt caggcttaag aaaattaggc cttgaatgtg
    13921 gcttttatat tccggaccgt attaaagagg ggtatgggct tagtgaagca acagtgatgt
    13981 tggcacataa gaaaggatat tctttgattc ttacggtgga taatggcata aaaagtaagc
    14041 cggctttagc actttctaaa gaattgggta tggatgtaat tgttacggat catcatacaa
    14101 tggatgaaga agtgaattgt gatattgttg tacatccaga tttaatggag ccatgctttg
    14161 aaaccttatg tggagctgga atagcctatg aatgtatgcg aaaacttggt gtagaggatg
    14221 attatctttt acagcttgca ggtcttgcga gtatttcgga tatgatggtt gtcaaagggc
    14281 aaacgcgagc tttgattcag aatgcattaa gatcaatcaa tgaaacgcat gaaaaacata
    14341 ttttttcttt agctacagat cgtgacttaa atgaaacaag tattggtttt caggtggttc
    14401 ctaaattaaa tgcgattgga agattgagta atcttgccaa cgtcaacaat gtggtccgat
    14461 attttttggc gcatgatgat gaaacaattt atactttggg gtcgcaaatt acacaaatga
    14521 atacgattcg taaacaaatg tcggatcaga tgcaaaaaac ggccttatta aagtgtaaat
    14581 cgaatgagga tatctatatc attgaagata caagttttca tgaaggaatc ataggtttag
    14641 tggcgggggc actttgttca agatttaata agccttgtat tgttctcgct aaaaatgaac
    14701 agggatataa ggcgagtatg cgtagtcctg aaggctttaa ctgcatggaa tttcttggcc
    14761 catacaaaca ttttgtcgta tttggtggac atgaaaatgc agcaggcttt tcattgaatc
    14821 taaatgagtt tgatttgttt gaagttttcg ttcatgcgcg tatcaaggaa tatacgtata
    14881 gtgtgaaaaa gaaggaaaca ttaattataa cggatgatga attgagttta gaaaccattc
    14941 aatccttaga tgtacttaga ccttttggtc cgggctttgt ttttcctagt tttgaaatta
    15001 ttcatcctca aattaagtcg ttgtatgatt ttcaaaatca taaacatcgt aaatatacat
    15061 tagaatctgg cttacagtgt atgcgtttta atcaaagtga agtagagtat aaaaaaagtg
    15121 tgaatgcgat tcattctttt ataggtacgg ctcaaatcaa tcaatatcag gggcgtaagc
    15181 aggtaaattt tgtgattgat gagattgtat atgagtagta aaatggaatc aattagaaag
    15241 aggtttagta tggatttaaa agattatata gaaagtattc cgggatttcc aaaagaagga
    15301 attattttta gagatgtgac acccattttg cagaatgcga gtgcaatgcg ttattgcgtg
    15361 gatcaattaa atgactttgt gaaaagtgtt ggtgcagatg ttgtgattgg tcctgaagca
    15421 cgtggctttt tatttggtgt gccagtggct ttagaaaata atgttggctt tgtacctgtt
    15481 agaaaacctg gaaagcttcc aagagaaacc gtaagtgaaa catatgatct agagtatggg
    15541 caagatgaac tttgtgttca tgcggatgcc attttaccag gacaaaaagt agttattatt
    15601 gatgatttgt tagctacagg cggtacagta gaagcgatta ttaaaatggt tgaacgtatg
    15661 caggctagtg ttaccggtgt ggcttttgtg attgaattgg atgatttaaa aggtagagaa
    15721 aagtttaaag atatccctgt ttgtgcatta acacattatg aaggggaata aatagtataa
    15781 ttttaagaaa gggggcgcat gaattatgag tcagaaaaag caagtaactt ttgaagagtg
    15841 tatggaaaat gtccaaacat atataaaacg cccagagaat attgagttga ttcaaaaggc
    15901 atatgatttt gcctatgaac atcacaaggg acaatttcgt aaaagcggag aaccttatgt
    15961 gattcacgtt attcaagtag caaatacgtt agcgttgatg cattgtggtc caaaaacgat
    16021 cgcggcaggt cttttgcatg atactgtaga agattgccaa ggcgtaacga cagatacaat
    16081 tacggaatta tttggtcagg aaatcgcaac tttagttgat gcggttacga agattggtgc
    16141 tattaaattt aaagatgaag aagaatattt agcgagtaat catcgtaaat tatttattgc
    16201 gatggcaaaa gatattcgtg ttattttaat taaactagca gaccgtttgc ataatatgcg
    16261 aactttgcag tatatgcgtc cggaaaaaca aaagaaaatt gcccgtgaaa cattaagtgt
    16321 ttatgctcct attgcgcatc gtttgggtat tagtgaaatc aaaaatgagt tggaagatct
    16381 ttcttttaag tatttagatt ataaaaaata tgaagaaatc aaagatttag taaaacaacg
    16441 tgaaagtgat cgtaacgaac aagttcatga aatgatttca gatattgaat cgattatgca
    16501 ggagtataat attcaatatc gtatttttgg tcgatcaaaa catttttata gcatctataa
    16561 aaagatggtg actaaaaata agcgttttga agaaattttg gatttattgg ccattcgaat
    16621 cgttacggat tccgttgtgc attgttatga aatactaggt tatatccatg ctaaatatag
    16681 accgattcca ggtcgcttta aagattatat tgcgatgcca aaagcaaata tgtatcagtc
    16741 tttgcataca acgatcgtag agcctgagca tggtaatatt tttgaaattc aaattcgtac
    16801 agaagaaatg gacgcgattg ccgagcgtgg tgttgcagcg cattggaagt ataaagaaaa
    16861 tcgtaattat actccagagt atgaacaaaa agagattgag gataaacttt cttggtttag
    16921 agatttttcg atgatgaccg atgaagaaag tgaagatcca cttgaatata tgaatgtact
    16981 tcaaaaagat atttttgaag cgaatgtata ttgtttaaca ccaagaggta aagtaattgc
    17041 cttgccttcg ggttcttgtc cgatcgattt tgcgtataga atccatacgg aggttggaaa
    17101 taaaacagta ggcgctaaag tcaacggggc tattgttcct ttaaatacgc ctttaaaaac
    17161 gggggatgtt gttgatatct taacaaacaa caattcggta ggtccaagtg cggattggat
    17221 caaaattgtt aagagtgggc atgcacgtaa taagattcgt actttccttc aaaaacaaga
    17281 acagcaatcg cgtaaggaga gtatcaaact aggacagtcg atgcttgagg atgagtttag
    17341 gcgtttaaaa ttagatccga aatcaatgga tcaaaaacga ttagaatcca ttttaacatc
    17401 cttatccttt aagagtgtgg atgatctata tgtaggtatt gccatgaagc gtgtttcgtt
    17461 gcagtcgatt gtggatcgtt tggttaaaaa ccgctcgaat atgcttgaag atcaagaaat
    17521 tatgaaaatt tacaacaagc agcctgcaca cgttacaaaa cattcaagtt gtggtgttat
    17581 tgtgcctgga gttgatacga ttgcggtatc tttggcaaat tgttgtagac cgattcctgg
    17641 agattcgatt attggatata tttcaaaagg gcaaggggtc aaggtgcata gagcggattg
    17701 tccaaatatt gtcaatgaaa agaagcgctt aattcctgta caatgggaag agggtctaga
    17761 tgaaaaacaa tatgaagtga atttgattat tcatagtgat gatcgaaatt acttgttgag
    17821 tgatattgtg acgactttac aacaatgcaa tgtctattta aaacatgtag attctgcggt
    17881 ggatgatggt aatttagagg caactactaa gttgacggtt tcagtgaaga atgcagcgca
    17941 tctacaaaac ttaatttcaa acttaaaaaa agtacgaagt gttaatgaag tcattcgtac
    18001 aattcaatag gaaggtgttt tttaaactgg ttcaaaagtc aaggacaaat atctgacagt
    18061 tcttgttaaa tgagtgtcac aaatagtgat gctcattttt ttatgcttgt aat
//
LOCUS       DBMDWL010000020        17693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1078_length_17693_cov_2.770521, whole genome shotgun sequence.
ACCESSION   DBMDWL010000020 DBMDWL010000000
VERSION     DBMDWL010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 17693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17693
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_17693_cov_2.770521"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="ACZPDN_02540"
     CDS             complement(<1..595)
                     /locus_tag="ACZPDN_02540"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR1297532.1"
                     /translation="MKICFVTGGTGGHIYPALALADKMKELDSSTEILFIGNDDRMEK
                     DLIPQNGYAFKALHTSGLVGNAFKKCMAVCQMFKAEGKAKKYLKEFKPDVVIGFGGYV
                     SAPVIMAAHSLGIHTVIHEQNSIVGKANQLVMKKVDAIITCYEKCNEVFPKEKIHMLG
                     NPRATIAKEAKFDEDYFKSLGLDLDKKTILIVMGSLGS"
     gene            complement(625..1374)
                     /gene="srtB"
                     /locus_tag="ACZPDN_02545"
     CDS             complement(625..1374)
                     /gene="srtB"
                     /locus_tag="ACZPDN_02545"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1297533.1"
                     /translation="MKKWIYRILLVVCLCVFGYSAYNLWDIYSQKQQVENETKQLEKS
                     VIKKKDKEKKQVLDPDWSKLKAENQDIVAWIYVPGCDISFPVVQTKDNQFYLNHTVSK
                     EYNDRGSIFLDANANGQFQDDNSIIYGHSVEGGGMFTLLKKYCDEDFFKSHPVFYLLT
                     PDANYKCHVFTFAKTTEDSVFYTTSFGDYRQETLDFMQQMSTYFNELDDIDANYVSLS
                     TCDLEYGFNSNHRFVLTGLMVKTSGDIVLKD"
     gene            complement(1444..2163)
                     /gene="nagB"
                     /locus_tag="ACZPDN_02550"
     CDS             complement(1444..2163)
                     /gene="nagB"
                     /locus_tag="ACZPDN_02550"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1297534.1"
                     /translation="MKVIVCENYDEMSAKAFGVMKELLDAKKDAVLGLATGSSPVGLY
                     KEMIQYHKDGYSYKDIKSYNLDEYVGIDRNDPQSYYTFMYENLFKDIDIDLNNTHVPY
                     GNTEADCKAYEDALSEVSIDLQVLGIGQNGHIGFNEPGTPFEELTHIVDLTENTREAN
                     ARFFDNDINKVPTQAISMGIGTIMKSKKVLLVANGENKADAVKAMIEGPIDTACAASA
                     LQNHADVVVVLDKAAASKLSK"
     gene            2353..3678
                     /locus_tag="ACZPDN_02555"
     CDS             2353..3678
                     /locus_tag="ACZPDN_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HIR1297535.1"
                     /translation="MPKQNPLLNIVKKQKEGKAVAIYSCCSSNAFVIEAAMETAKKQD
                     SCVLIESTANQVDQNGGYSGMTPQDFFNFCHEKAKEAGLSSDRIFLGGDHLGPLTVSN
                     KPETEAMEYAKTLVHDYVRAGFTKIHIDTSMKVADDDPNTRLSDETIARRGATLAKVC
                     EEAYQELLQENPEAIHPVYIVGSEVPIPGGAQEENAGMQVTKPEDFKSTVATFEKAFN
                     DMGIANAWNHVIAAVVQPGVEEKDAGCEEYDRERAKDLMASIKDFDKLVFEGHSTDYQ
                     TKFKLRELVEDGVGILKVGPGLTYAAREGIFSLCMIEEELAAVYGFETSHFREELDKA
                     MLANPGKWAPYYHGTENEIAFKRKYSFSDRCRYYLPQENVKNALAKLLANLKEHPVPL
                     PLLSQYMPFQYTLVREGKLNNTPEDLIKARVMYTIEEYTYASHQEELVR"
     gene            complement(3747..4295)
                     /locus_tag="ACZPDN_02560"
     CDS             complement(3747..4295)
                     /locus_tag="ACZPDN_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297536.1"
                     /translation="MAQGRKTRQKQQTDLKGNVSGWHVYKDDRGRAVYYDVFSKTGYI
                     LANNEERYKQFSMRFVMGLIGFILILMFNMPIWLCVLAGVGVYAFLEFRFRKFLATLP
                     RIENFKCNEKMKSEASIKTLETNKVILKMVLLIALSVLIVLNALNERYTGWMLYLNYV
                     ISALALIFALCHAKELISRKSK"
     gene            complement(4305..4886)
                     /locus_tag="ACZPDN_02565"
     CDS             complement(4305..4886)
                     /locus_tag="ACZPDN_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297537.1"
                     /translation="MADKKYPVLYATSVKGTIFRHCGIYNTIYFNIYNNKELEDKPYY
                     LEYMEETREEVYKAIQNKFTMSQPLKVTNDHKVFIIFRGNIDMRDVKTFCKMMLQELE
                     YFTEGVHKADYAELETMFMEIGRAPTFMKASKVGEKLTQTDILDKIMVRMDGHDQPQD
                     NGCLTPYTDYVDFKEEEKRQNLKKEELEEVVEW"
     gene            complement(5030..5905)
                     /locus_tag="ACZPDN_02570"
     CDS             complement(5030..5905)
                     /locus_tag="ACZPDN_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1297538.1"
                     /translation="MESNAMTSYKDTTPAARLDKQTLNKMVWRSCQLQAAFNYERMQS
                     AGWLWAMLPGLEKIHTNKDDLAASMTHNMDFLNTHPFFVTFVMGIVLSMEQQKTDIQT
                     IRSVRISTAAPLGGIGDALFWYTLIPITAGLTANMAIDGSIMGPILYFVIAFGVEMVL
                     RYALMYWSYNLGLTAVKMMTANAKAFTHAASVLGVFVVGALISNYGGGTKLGISIANG
                     EGDPIVIQNYLNMILPCLIAFAFTMMCYVLIKKKNWTPVKCIALFLVIGIVGAAFGIW
                     AGGYTPLVPVPWTVI"
     gene            complement(5895..6686)
                     /locus_tag="ACZPDN_02575"
     CDS             complement(5895..6686)
                     /locus_tag="ACZPDN_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIR1297539.1"
                     /translation="MNIVEIVLLAIVTFFFAIDQFSLTEIVYRPIIACPIIGAILGDV
                     NTGLVVGGTYELMMVGNMPVGGAQPPNAVLGSVVAMIFAVKAKMDINAALGACMIFAT
                     FGQYVVTLTFTIMSGLMAKADKAANEANPKGITAVLNTSMAILGTLFAVIAILAYVGG
                     TAASGALQSLSENASWFMGGLSAAGGMMRFVGFAVLLKIMLSNDLWGWLLAGFACALL
                     FGNVEATASACLILVAFIGVAIALLDFKITSIETKAAQGGVSDGI"
     gene            complement(6710..7186)
                     /gene="agaB"
                     /locus_tag="ACZPDN_02580"
     CDS             complement(6710..7186)
                     /gene="agaB"
                     /locus_tag="ACZPDN_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HIR1297540.1"
                     /translation="MPNILLTRIDNRLIHGQVATQWSGVLGANLLLVANDAVAKDEFR
                     QGLMNMAAPAYAQTRYFSIQKTIDIIGKASPAQKIFIICETPQDVLRLVEGGVPIKKC
                     NIGNMHMSEGKRQVATTVAVNDDDVAAFKKLQELGVELEIQRVPDIAPEDINKLWS"
     gene            complement(7425..8591)
                     /locus_tag="ACZPDN_02585"
     CDS             complement(7425..8591)
                     /locus_tag="ACZPDN_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1297541.1"
                     /translation="MDYKGKIEASIQAHLDAYMDIVKTLYDNPETGFKEYETQKVLVK
                     YLKDAGFDVKSGVVVDTDFVAEYKSGKEGPTIAYMCEYDALPEVGHGCGHNLIAGIGI
                     AAGEALKSVIDEIGGTVRVVGTPGEENFGGKVKMSEAGVFDDVDAALMVHPSTENGVG
                     SRSLAILPIKYEFFGKNAHGCRPQEGHSALDAAISTYTQISMLRQFVTPGSYIHGIIR
                     DGGLAANVIPAYASMEYYFRAPTMAYCKEIAARAEDCVKGSCIASQTTYKTSMYECPY
                     EDTKINYTLADMLTEKYKELGVEQINPVDEVPCGSTDVGSVSYVCPTIQGTIKIADCT
                     VAGHSKEMAAATISEDGKAGLVTAATAIALIGLDLIVKPERLEKAKKEFKEGTC"
     gene            complement(8689..9120)
                     /gene="agaF"
                     /locus_tag="ACZPDN_02590"
     CDS             complement(8689..9120)
                     /gene="agaF"
                     /locus_tag="ACZPDN_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HIR1297542.1"
                     /translation="MIGLIVTGHGHFASGITTSLNLIAGDAKDYVAVDFEATDSTDDL
                     AKKLTDAMDSLKDCEGIIVLSDLAGGSPFKTAVEIGFPRGNVEVIAGTNLGMLVEINL
                     TRTFSEDVHALAESACNVGKDQVMRYVFQHIQQNDDLSDGI"
     gene            complement(9157..10335)
                     /locus_tag="ACZPDN_02595"
     CDS             complement(9157..10335)
                     /locus_tag="ACZPDN_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1297543.1"
                     /translation="MFGKEKSTWEELSGVFTAQEIYQQPATWAKTIAQIKNEKEALKK
                     FMEPVLSDPDCDIVFTGAGTSEYVGNALYSYVNRYTGYHAKSYASTDLVETPENYLSR
                     NKKTLLVNFGRSGNSPESVGSVQVADEVCKENVYHLFITCNKDGALAKAASTRDNAYD
                     INLTPETHDQSFAMTSSFSNMMLAALLCFRLDDIDEAAKEMESVIAQGQKFVDEGYKV
                     CEDLVSDFAEFNRIVYLGANTLKGIAQESQLKICELTAGTVTTTFDSPMGFRHGPKSV
                     INDECLTVVYLSDDEYQRQYEYDIIKEMSGQRKKNRLVAVSSHADAEIEGLVDTFINF
                     DLDAPKDNIFLGFDYILVAQVIALFKSLSYKITPDNPCPTGEVNRVVKGVTIYPYTAK
                     "
     gene            complement(10475..11326)
                     /locus_tag="ACZPDN_02600"
     CDS             complement(10475..11326)
                     /locus_tag="ACZPDN_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1297544.1"
                     /translation="MAYILENDVLKLECTEKGGEMLHLVKKSTGRETLYQGDQGWSGR
                     NPSLFPMVGNTYTKDYEIDGKKYAMKNHGLIRYATLKGESKEDKLVFSLDANEETLAQ
                     YPFNFHFEIGYKLDGNKVLINYHIKNKDEKDMPFGFGLHPGFKLDDEFSTYTLDFEKE
                     ENAKQLLFDPSYEKNVEYQDVAFKEWKLSRKDVKKYATIIYKDLKSSFVTLKHGDDEV
                     VRVSIEGYPYLALWTHDSESDFLCIEPWYSHGDFEKETPDFYHREGTMILKPNEEWSC
                     SYWIEVL"
     gene            complement(11350..>11785)
                     /locus_tag="ACZPDN_02605"
     CDS             complement(11350..>11785)
                     /locus_tag="ACZPDN_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR1297545.1"
                     /translation="RNGNYFGTPYSKYAHDIYFHLYLNSPIFKPYENDNVLNLLVFCE
                     GKDKEQFRPLIKDLGIVEFFDCFEIRANQIDKATAVKKIMDFHKFDTTYCFGDGTNDV
                     DMLKMADVGVAMGNACDALKEVSDVVIGRVDEDGIYNFLMRK"
     assembly_gap    11786..11795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11796..12110)
                     /locus_tag="ACZPDN_02610"
     CDS             complement(<11796..12110)
                     /locus_tag="ACZPDN_02610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1297546.1"
                     /translation="MNGYFFDMDGTLYNNKFHEVSEKTFKMLNELQRQGHFVALCTSR
                     CQTELKNLPSCMRNFQFDCMISDGGALILDRHKEVLEMNCISKATMKTIDSFCKEHGL
                     VYR"
     gene            complement(12324..13169)
                     /locus_tag="ACZPDN_02615"
     CDS             complement(12324..13169)
                     /locus_tag="ACZPDN_02615"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1297547.1"
                     /translation="MSRVGIVAEGGGTKAAYSAGVLKCLLEHDIVLPYCVGISAGTEI
                     LLSYVSKQVDRLEVTGIDAPCQKGVVGLRPFLKEGSIFGIEATYDFIESRVPLDLEAF
                     QNSETQMEVGLYNLKTHKVEYFDKSHIDKDETLIKASCALLLLAKPYEFKGEMWMDAG
                     LVDMISIEQSLRAGNDKHIVISTKEEGYVRNPAPKWQLWLSNLVYKDKQITDDLRNRH
                     IRYKEQWDKIAELEKQGKALVLRPHKDLGVTRYTTDPEKLGPWFQLGYDETLERMDQI
                     KEFIK"
     gene            complement(13162..14325)
                     /locus_tag="ACZPDN_02620"
     CDS             complement(13162..14325)
                     /locus_tag="ACZPDN_02620"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR1297548.1"
                     /translation="MDYAKEALRLHAKWNGKLETVPKMKIENKDDLSIAYTPGVAAPC
                     LEIEKDKKNSYVYTGRSHTVAVISDGSAVLGLGNIGPEAGMPVMEGKCVLFKTLGNVD
                     AVPLCLNTQDTDELVQIISSLEPSFGGINLEDIAAPRCFEVEKRLQEKMNIPVFHDDQ
                     HGTAIVVCAALVNALKLAEKQNPTIVINGAGSAGVAIATLILKIGLGNVILVDKQGIL
                     TADMDLTSGQRGLIDKLNKEGKTGSLKDALVGADVFIGVSAGHIVSKEMIASMNEKAI
                     VFPMANPVPEIEPDDAKEAGAYIVGTGRSDHPNQINNVLAFPGIFKGALKAGATCIND
                     AMKEAAVYAISSMVTEDMLDSEHIIVSALDPRVADVVAQAVADAAKESGVVRE"
     gene            complement(14335..15531)
                     /gene="pepT"
                     /locus_tag="ACZPDN_02625"
     CDS             complement(14335..15531)
                     /gene="pepT"
                     /locus_tag="ACZPDN_02625"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1297549.1"
                     /translation="MAVEKRLMHYASFDTQSSEESTSAPSTEKQLVLARELKKECESL
                     GFDSVELTDTGIVYAYLNANTDKKMDRIGFIAHMDTASEITGANVKCRVISKYDGNTI
                     QLNEEYSMCKEEFPALANCIGDDLIVTDGTTLLGADDKAGIAIILEAMEQLIQSEKEH
                     GFIAVAFTPDEEVGRGVENFELDKFAVDYAFTIDGDRIDSVDYETFNAAQAVVTFDGT
                     SIHPGDAKDRMVNASLLAMEYASSLPKKQTPSHTQGRQGFYHLVGMEGICEEAKLTYI
                     LRNHSKQSFIAQKQKMEAVKDKMNEKYGNRVHVTIRDQYENMRQYMHGDMRSVNKAKY
                     ALRQCDVTPVSTPIRGGTDGAMLTARGLICPNLGTGSFNHHGRFEFASIQKMEKMVEI
                     VLKIVE"
     gene            complement(15546..>16101)
                     /locus_tag="ACZPDN_02630"
     CDS             complement(15546..>16101)
                     /locus_tag="ACZPDN_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA2 domain-containing protein"
                     /protein_id="HIR1297550.1"
                     /translation="SCTIVGLMYSEYGVKMPETIAKLIAYGMISDTMNFNSPTCTSID
                     HTLAKRLSSEYDLDFDAMAKELFENTATIRGKEFKNILYNDVKEYMLSGYHIAVSQVF
                     TFDLSIVDEIKEDFLKYMEEQNKVHEYDLMLMVITNVEGRGSRFLYVGKLSYFVRDVV
                     EEFKDQGFVSRKKQIVPRLAQELA"
     assembly_gap    16102..16111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16112..17127)
                     /locus_tag="ACZPDN_02635"
     CDS             complement(<16112..17127)
                     /locus_tag="ACZPDN_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1297551.1"
                     /translation="MSLPIYVIGHKNPDTDSICAALALAELKRQTGINATAARLGTLN
                     PETKFILDKLNVNMPVLLTTARSTLEEIEIDEAVTIEEGQTIRRAWDLCLENHVKTLY
                     VVDDKNEYKGMVTLGDVSKVQMQDLNITSELLKETPLENLVASVKGSFILKGNLERSG
                     FVRIADKRLMERDLKGAIMVLNDHEDSMIKSMAKGCAVIVVAENFVPNSYIIEMAKQM
                     GVTLISTPYNIMKIIQMIYRSIPVELIMTPANKVIRFNKSEYVEDVEREMLKTRHSSY
                     PVVFQNKILGSVARYNLLKAEKKRFILVDHNEKKQSISDIETADIVEIVDHHRIGDIE
                     TDRP"
     gene            complement(17207..17491)
                     /locus_tag="ACZPDN_02640"
     CDS             complement(17207..17491)
                     /locus_tag="ACZPDN_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1297552.1"
                     /translation="MSNVINKKDLAEKLAEEFGCSKKDATAYVQFVFDTVADTLKDNG
                     TVDVFGFGKFSISERAARTGINPATKEKIEIAASKSVKFKASKALKDAVK"
BASE COUNT         5763 a         3706 c         2317 g         5887 t
ORIGIN      
        1 tagaacctaa agagcccata acaataagaa tggtcttttt atctaaatct aagccgagtg
       61 atttaaaata gtcttcatca aattttgctt ctttagcaat tgtagccctt ggatttccaa
      121 gcatgtgaat cttttccttt ggaaaaacct cattgcactt ttcataacaa gttataatcg
      181 catccacttt tttcataaca agctgatttg cttttccaac aatagaattt tgttcatgga
      241 ttacagtatg gatacctaaa gaatgagctg ccataataac aggtgcactc acatagcctc
      301 caaagccaat aactacatct ggtttgaatt ctttcaaata tttttttgcc ttaccttctg
      361 ctttaaacat ctgacaaaca gccatacatt ttttaaatgc atttcctacc aatccagaag
      421 tgtgtaaagc cttaaacgca tatccatttt gaggaatcaa atccttttcc atgcgatcat
      481 catttccaat aaataatatt tccgttgaag aatcaagttc tttcatttta tccgccaaag
      541 ctaaagccgg ataaatatgt ccacccgttc ctcctgtcac aaaacaaatt ttcatattaa
      601 aaacctcacc aagtatatca tatattaatc ttttaaaaca atatcgccac ttgtctttac
      661 cataaggcca gttaagacaa aacgatgatt cgaattgaat ccatattcta aatcacaagt
      721 agataaagat acataattcg catctatatc atctaattca ttaaaataag tcgacatttg
      781 ttgcataaaa tccaacgtct cttgtctata atcaccaaat gacgttgtat agaatacact
      841 atcttctgtc gttttcgcaa atgtaaaaac atgacactta taattcgcat ctggtgttaa
      901 taaataaaat actggatgcg acttaaaaaa atcttcatca caatactttt ttaataaggt
      961 aaacatacct ccaccctcta cagaatgtcc atatataatt gaattatcat cttgaaattg
     1021 tccattcgca ttcgcatcca aaaaaatcga acctctatca ttatattctt tactcacagt
     1081 gtgattcaaa taaaattgat tatcttttgt ttgcacaacc ggaaaagaaa tatcacaccc
     1141 aggaacatag atccatgcga caatatcctg attttctgct ttaagtttgc tccaatccgg
     1201 atctaatact tgcttttttt ctttgtcctt ttttttgatt acactttttt ctaattgttt
     1261 agtttcattt tctacttgtt gtttttgtga ataaatatcc cataaattat atgcactata
     1321 tccaaataca caaagacaaa caactaaaag aattctatat atccattttt tcatacatgt
     1381 tcattttaac acaaataaaa aggatagaga aatgaattcc ctatccaaca aagacaaata
     1441 taattatttg cttaatttgc tagcagccgc tttgtctaat acaacaacta catctgcgtg
     1501 gttttgcaat gcacttgcag cacatgctgt atcgattggt ccttcaatca tagctttcac
     1561 tgcatctgct ttattttcgc catttgccac taataatact tttttagatt tcataattgt
     1621 tccaattccc attgaaatag cttgtgttgg aactttattg atatcattat caaagaaacg
     1681 agcatttgct tcacgagtat tttctgtcaa atcaacaatg tgtgttaatt cttcaaatgg
     1741 agtaccaggt tcattgaatc caatgtgtcc attttgtcca attcctaata cctgtaaatc
     1801 aatagatact tcacttaaag catcttcata agctttacaa tctgcttctg tatttccata
     1861 aggaacatgt gtgttattca aatcgatatc gatatctttg aataagtttt catacataaa
     1921 tgtatagtaa gattgaggat cattacgatc gattcctaca tattcatcta agttataaga
     1981 tttaatatct ttatagctat atccatcttt gtggtattgg atcatttctt tatataatcc
     2041 aaccggactc gatcctgtag ccaatcctaa tacagcatct ttttttgcat ccaacaattc
     2101 tttcatgaca ccaaacgcct tagctgacat ttcatcataa ttttcacata cgataacttt
     2161 catttgtatc tcctccatat atctctttta ctattatagc atagcgcttt caaaataaaa
     2221 taaacatttt agtaatgctt ttgtcatttt taacaaatta gtaatgtttg cactttttaa
     2281 ttgactttat gttaaaaagg agaaaaaaag ttaaacatac tgtataataa aatcagttaa
     2341 ggaggacata taatgcctaa acaaaatcca ttattaaaca ttgtaaaaaa gcaaaaagaa
     2401 ggtaaagccg tagcaatcta ttcatgctgc tcaagtaatg cattcgtaat tgaagctgcg
     2461 atggaaaccg caaaaaaaca agattcatgt gttttaattg aatcaacagc aaaccaagta
     2521 gaccaaaatg gtggttactc tggaatgaca cctcaagatt tcttcaactt ctgccatgaa
     2581 aaagcaaaag aagctggatt atctagtgat cgtattttct taggtggtga tcacttagga
     2641 ccattaacag tttcaaacaa accagaaact gaagcgatgg aatacgcaaa aacattagtt
     2701 cacgactatg tacgtgctgg atttactaaa attcatattg atacaagtat gaaggttgca
     2761 gatgatgatc caaacactcg tttaagtgat gaaactatcg ctcgtcgtgg agctacttta
     2821 gctaaagtat gtgaagaagc ttatcaagaa ttacttcaag aaaatccaga agcaattcac
     2881 ccagtataca ttgtaggtag tgaagttcca attccaggtg gagcacaaga agaaaatgcg
     2941 ggtatgcaag tcacaaaacc tgaagatttc aagagtacag tagctacatt tgaaaaagca
     3001 tttaatgaca tgggaatcgc aaatgcatgg aaccatgtaa ttgcagctgt agttcaacct
     3061 ggtgttgaag aaaaagatgc tggatgtgaa gaatatgata gagaacgagc aaaagattta
     3121 atggcaagca ttaaagattt tgacaaatta gtatttgaag gacactcaac agactatcaa
     3181 actaaattca aattacgtga attagtagaa gatggtgtag gaattttaaa agttggtcca
     3241 ggacttactt atgccgctcg tgaaggaatc ttctcattat gcatgattga agaagaatta
     3301 gctgctgttt acggatttga aacaagccac ttccgtgaag aattggacaa agctatgttg
     3361 gcaaacccag gtaaatgggc accatattat catggaacag aaaatgaaat cgcatttaaa
     3421 cgtaaatatt cattctctga tcgttgccgt tattatttac ctcaagaaaa tgtcaaaaat
     3481 gctttagcta aattattggc aaacttaaaa gaacacccag taccattacc attgctaagt
     3541 caatatatgc ctttccaata tacattggta cgtgaaggta aattgaacaa cacacctgaa
     3601 gatttaatta aagctcgcgt tatgtataca attgaagaat acacttacgc tagtcaccaa
     3661 gaagaattag ttcgttaata tcaatgtgcg catcttaaag atgcgcattt ttttatgcaa
     3721 caaaaaaaag gttcaaccga acccttttac ttactctttc tagaaattaa ttcttttgca
     3781 tgacataacg caaagatcaa tgctaatgca ctaatcacat aattcaaata taacatccaa
     3841 cctgtgtagc gttcatttag tgcatttagt acaatcaata cagataaagc gattaataaa
     3901 accattttta aaatcacttt atttgtttct aatgttttaa ttgaagcttc actcttcatt
     3961 ttttcattac acttaaagtt ttcaatacga ggtaaagttg ctaaaaactt tctaaatcta
     4021 aattctaaaa aagcatatac accaacacct gctaaaacac ataaccagat aggcatatta
     4081 aacatcaata tcaagataaa tccaattaat cccatcacaa agcgcatact aaattgttta
     4141 tatctttctt cattgtttgc caagatatat cctgtttttg agaatacatc ataataaaca
     4201 gctcttccac gatcatcttt ataaacgtgc caaccagata catttccttt taaatctgtt
     4261 tgttgttttt gtcttgtctt tcttccttgt gccataatat ctccttacca ctcaactact
     4321 tcttcgagtt cttctttttt aagattttgt cgtttttctt cctctttaaa atcaacataa
     4381 tccgtatatg gtgtcaaaca accattatcc tgaggttgat catgaccatc catgcgcacc
     4441 attattttgt caagaatatc tgtttgtgtc aacttttctc caacttttga agctttcata
     4501 aaagtaggag cacgcccaat ctccataaac atcgtttcta attctgcata atccgcttta
     4561 tgcacacctt ccgtaaaata ttccaactct tgcagcatca ttttacagaa agttttcaca
     4621 tcacgcatat caatattgcc tctaaaaata atgaaaacct tatgatcatt tgtcactttt
     4681 aatggttgag acattgtaaa tttattctgg attgccttat aaacttcttc tcttgtctct
     4741 tccatatatt ctaaataata aggtttatcc tctaattcct tattattata tatattaaaa
     4801 taaatagtat tataaattcc acaatgtcta aaaattgttc ctttaacact tgtggcataa
     4861 agaactggat attttttatc agccataatt tgtacctcct taatttcatt tttaaaccat
     4921 taaattataa tatttaatga cctaaaaata aaattaaagg ttgtaaggat ataatccgtt
     4981 acaaccttat atttgagaga aaatattcta acttcgttag attagcctac tagataactg
     5041 tccaaggaac tggtactaga ggtgtgtatc caccagccca gataccgaat gcagcaccaa
     5101 caataccgat tactaagaat aatgcgatac attttactgg agtccagttt ttcttcttaa
     5161 tcaatacata gcacatcata gtgaatgcga atgcgattaa gcaaggtaaa atcatattta
     5221 agtagttttg gataacaatt ggatcacctt caccgttagc aatagagata cctaattttg
     5281 ttcctccacc atagttagaa atcaaagcac caacaacgaa tacacctaat acactagcag
     5341 cgtgagtgaa tgctttagcg ttagcagtca tcatcttaac agcagttaaa cctaagttgt
     5401 atgaccagta cattaaagca taacgtaata ccatttcaac accgaatgcg ataacgaagt
     5461 ataagattgg tcccatgata cttccatcaa tagccatgtt tgcagttaaa ccagcagtga
     5521 taggaattaa tgtataccag aacaatgcat caccaatacc acctaatgga gcagcagttg
     5581 agatacgtac agaacgaatt gtctgaatat ctgttttttg ttgttccatt gataatacga
     5641 tacccataac aaatgttacg aagaatgggt gagtattaag gaaatccatg ttgtgagtca
     5701 tagatgcagc taagtcatct ttattagtgt ggattttttc caaacctggt aacatagccc
     5761 ataaccaacc agcagattgc atacgttcgt agttgaacgc agcctgtaac tggcaagaac
     5821 gccataccat tttatttaaa gtttgtttat ctaatcttgc agcaggagta gtatccttgt
     5881 aagaagtcat tgcattagat tccatcactt acacctccct gcgcagcttt tgtttcaata
     5941 gaagtaatct tgaagtctaa taatgcgatt gcaacaccga tgaatgcaac taagatcaag
     6001 caagcagaag ctgtagcttc aacgttacca aatagtaatg cgcaagcaaa accagctagt
     6061 aaccatcccc acaagtcatt agaaagcata atctttaaca atacagcgaa acctacgaaa
     6121 cgcatcattc caccggcagc acttaaacca cccatgaacc agcttgcgtt ttcacttaaa
     6181 ctttgtaaag caccagaagc agcagttcca ccaacatatg ctaaaatagc gattacagca
     6241 aataaagtac ctaagatagc cattgaagta tttaatacag cagtgattcc tttagggtta
     6301 gcttcattag cagctttatc agctttagcc attaaaccag acatgattgt aaatgttaat
     6361 gttacaacgt attgtccgaa tgtagcgaaa atcatacaag ctcctaaagc agcattaata
     6421 tccatttttg ctttaacagc gaaaatcata gcaacaactg atcctaatac agcgttagga
     6481 ggctgtgctc caccaactgg catgttacca accatcatta actcatatgt accaccaaca
     6541 actaagccag tgtttacatc acctaagatt gcaccaataa ttggacaagc aatgatagga
     6601 cgataaacga tttcagttaa tgagaattgg tcgatggcaa agaagaatgt tacaattgcc
     6661 aataacacaa tttctacaat attcattttt cattttctcc ttttttggat taactccaaa
     6721 gtttattaat atcttcaggt gcgatgtcag gtacacgttg aatttctaat tcaactccta
     6781 attcttgcaa tttcttgaat gcagcaacat catcgtcatt tacagctact gtagtagcga
     6841 cttgacgttt accttcagac atgtgcatgt ttccaatatt acatttttta attggaacac
     6901 caccctcaac tagacgtaat acatcttgag gtgtttcaca aatgatgaag atcttttgag
     6961 cagggcttgc ttttccaata atgtcgattg tcttctgaat tgagaagtaa cgagtctgag
     7021 cataagctgg agcagccatg ttcatcaaac cttgacggaa ttcatctttt gcgacagcgt
     7081 catttgcaac taataataag ttagctccta gaactccact ccattgagtt gcaacttgac
     7141 cgtgaatcaa acgattgtcg attcttgtta ataagatatt tggcatattc tctctccttc
     7201 ctgatagcgc tttcttcttt aaagaataac tatcttgact atattataca ttttctattc
     7261 ataatttctt tacacatttt aagaaattgt ttgcactttt tagtaatttt ttatttttta
     7321 tcactaacaa ttataaaaaa tggcaaaaaa ataacataat tatcacaaaa tcggtttaaa
     7381 aatccatttt gaaaaccttt tcacaaaata aaagaacatg ctaattagca tgtcccttct
     7441 ttaaactctt tttttgcttt ttctaacctt tctggtttaa caatcaaatc taaaccaatt
     7501 aacgcaatcg ctgtagccgc tgttactaat ccagcttttc catcctcact aattgttgca
     7561 gctgccattt ctttagaatg accagccaca gtacaatcgg caattttaat cgttccctga
     7621 attgtagggc aaacataact tacacttcct acatctgtgg aaccacatgg aacttcatct
     7681 actggattaa tctgttctac acctagttct ttatattttt ctgtcaacat atccgctaaa
     7741 gtatagttga tctttgtatc ttcataagga cactcataca tacttgtttt atatgttgtt
     7801 tgacttgcaa tacaagaacc ctttacacaa tcttcagcac gagccgcaat ttctttacaa
     7861 taagccattg taggagctct aaaataatat tccatacttg cataagcagg aattacattg
     7921 gcagctaaac caccatcacg aataattcca tgaatatagg atccaggcgt tacaaattga
     7981 cgtaacattg aaatctgtgt atatgtgcta atcgctgcat ctaatgcact atgaccctct
     8041 tgaggacgac atccatgcgc attcttacca aagaattcat atttgatagg taaaatagcc
     8101 aatgatcttg atccaactcc attttcagta cttggatgta ccattaaagc ggcatcaaca
     8161 tcatcaaaga caccagcttc actcattttg actttaccac caaaattctc ttcaccaggc
     8221 gttcccacaa cacgaacggt tccaccaatt tcatcaataa cactcttcaa ggcttcacca
     8281 gccgcaatac caataccagc aattaagtta tggccacatc catgtccaac ttctggtagt
     8341 gcatcatatt cacacatata ggcaatcgtt ggtccttctt tacctgattt atattcagcc
     8401 acaaaatctg tatcaaccac tacgccagac tttacatcaa agcctgcatc tttcaaatat
     8461 ttgaccaata ccttttgtgt ttcatattcc ttaaaacctg tttctggatt atcgtatagt
     8521 gtttttacaa tatccatata tgcatctaaa tgtgcttgaa tagatgcctc aatttttcct
     8581 ttataatcca tataagatac ctccatttgt gaaaatatca taccgcattt tcaaacattt
     8641 gaaaagtttt tacaaaagaa aaagccaggc aaaagcctag cttaattatt agataccatc
     8701 tgataaatcg tcgttttgtt gaatgtgttg gaatacataa cgcataactt ggtcttttcc
     8761 aacattgcat gcagattcag ctaaagcatg tacatcttct gagaaagtac gagttaagtt
     8821 gatttcaact aacataccta agtttgtacc tgcaataact tctacatttc ctcttgggaa
     8881 accaatttca acagccgtct taaatggaga accaccagct aaatcactta atacaatgat
     8941 tccttcgcag tcttttaatg aatccatagc gtctgttaat ttttttgcta aatcatctgt
     9001 tgaatctgta gcctcaaaat ctacagcaac ataatctttt gcatctccag caatcaagtt
     9061 taaacttgta gttataccag atgcaaagtg gccatgacca gtaacaatta atcctatcat
     9121 cttctttttt ctcctctagt tttctcctct aaattcttac tttgcagtat atgggtaaat
     9181 agttacaccc ttaacaacac ggtttacttc tcctgttgga catggattgt ctggtgtaat
     9241 cttataactc aatgatttaa ataatgcgat aacttgagca actaaaatat agtcaaatcc
     9301 taagaaaata ttatcttttg gtgcatctaa atcaaagtta atgaatgtat ctaccaatcc
     9361 ttcgatttca gcatctgcat gactagaaac agcaaccaaa cggttctttt tacgttgtcc
     9421 actcatttct ttaataatgt catattcata ttgtctttga tattcatcat ctgataaata
     9481 aacaactgtt aaacattcat cattgataac tgatttaggt ccatgacgga atcccattgg
     9541 actatcaaat gtagttgtaa cagtacccgc agtcaattca caaatcttta attgactttc
     9601 ttgagcaata cccttcaatg tatttgcacc taagtaaaca atacgattga attcagcaaa
     9661 gtcagatact aaatcttcac atactttgta tccttcatca acaaattttt gaccttgagc
     9721 aattacactt tccatttctt ttgcagcttc atcaatatca tctaaacgga aacataataa
     9781 tgcagccaac atcatgttag agaaacttga agtcatcgca aatgattggt catgagtttc
     9841 aggtgtcaaa ttaatatcat aagcattgtc acgagtagaa gccgcttttg ctaaagctcc
     9901 atccttgtta catgtaatga ataaatgata cacattctcc ttacatactt catcagcaac
     9961 ttgtactgaa ccaacacttt caggagagtt tcctgaacga ccaaagttca ctaataaagt
    10021 ctttttattt cttgataaat agttttctgg agtttctact aaatctgtag aagcatatga
    10081 ttttgcatgg taaccagtat aacggttaac atatgaatat aaagcattac ctacatattc
    10141 actcgttcca gcacctgtaa atacgatatc acaatctgga tcacttaata caggttccat
    10201 gaatttttta agagcttcct tttcattttt aatttgagca attgttttag cccaagtagc
    10261 aggttgttgg taaatttcct gtgcagtaaa aacaccactt aattcttccc aagttgattt
    10321 ttcttttccg aacatttttc tttctccttt tgaactaatt tcattataac tatttttaac
    10381 taaaataaaa gattttggat aaaagtaaac taaaaagtgc aaatttgttc tttaaaaaga
    10441 caaatttaca ctaaaaaaga aggatttctc cttcttaaag cacctcaatc caatatgaac
    10501 aagaccactc ttcatttggc tttaaaatca tagttccttc tctatgataa aaatccggtg
    10561 tttccttttc aaaatcacca tgcgaatacc atggttcaat gcataagaaa tccgattcac
    10621 tatcatgtgt ccataaagct aaatatggat atccttcaat cgatacacga acaacttcat
    10681 catctccatg ttttaaagtc acaaaacttg attttaaatc tttataaata attgtcgcat
    10741 acttctttac atcttttcta gacaatttcc attctttaaa tgcgacatcc tgatattcta
    10801 cattcttttc gtagctagga tcaaacaaca attgttttgc gttttcctct ttttcaaaat
    10861 ctaaagtata tgttgaaaat tcatcatcca acttaaatcc aggatgcaaa ccaaatccaa
    10921 aaggcatatc cttttcatcc ttgttcttaa tatgataatt gatcaatact ttgtttccat
    10981 ccaatttata cccaatctca aaatggaaat taaagggata ttgagctaac gtttcctcgt
    11041 tcgcatccaa gctaaataca agcttatctt ctttactttc accctttaaa gtcgcataac
    11101 gaatcaagcc atgatttttc atcgcatatt ttttaccatc aatctcataa tcctttgtat
    11161 acgtatttcc taccattgga aatagagaag gatttcttcc agaccaccct tgatctcctt
    11221 gatataaagt ttcacgccct gtactcttct ttactaagtg tagcatttcg ccaccctttt
    11281 ctgtacactc caacttcaat acatcattct ctaaaatata agccattcta ttctcctcct
    11341 attcagcttt tattttctca ttaagaaatt gtagatgcca tcctcatcca ctcttccaat
    11401 cacaacatca ctaacttctt ttaatgcatc acacgcattc cccatcgcaa caccaacatc
    11461 cgccattttc aacatatcca catcatttgt tccatcccca aaacaatatg ttgtatcaaa
    11521 cttatgaaaa tccataattt ttttaacagc cgttgcctta tcaatttgat tagcacgaat
    11581 ctcaaaacaa tcaaaaaatt ctacaatacc caaatctttg atcaaaggcc taaattgttc
    11641 tttatctttt ccttcgcaaa agacaagaag atttagtaca ttatcattct catatggttt
    11701 aaatattgga ctatttaaat acaaatggaa ataaatatcg tgtgcatact tcgaatacgg
    11761 cgtaccaaaa taatttccat tccttnnnnn nnnnnacgat acactaaacc atgttcctta
    11821 caaaaagaat ctatagtttt cattgtggct ttagaaatac aattcatttc aagaacttct
    11881 ttatgtctat ccaaaatcaa agccccgcca tcactgatca tacaatcaaa ttgaaaattt
    11941 cgcatacaag aaggaagatt ctttaattct gtttgacaac gacttgtaca taaagctaca
    12001 aagtgccctt gtctttgtag ttcatttaac atcttaaatg ttttttcact cacttcatgg
    12061 aacttgttat tgtataaagt tccatccata tcaaaaaaat aaccgttcat aacttaataa
    12121 tatcctcttt ctaatttttt taaaagaatt ttcgattttt aataataaat tcacattgac
    12181 tctttacaat tataaatgtg actacattct tcctttctag taggtaactc aattggcact
    12241 ttctcaagag tagtcactat cgccacaaca aacctcctta aatgattgtt gtggcaaaag
    12301 aaaaacctgg aaatccaggt tttttattta ataaattcct taatttgatc catacgctct
    12361 aaagtttcat catatcctaa ttgaaaccaa ggacccaatt tttctggatc tgtcgtataa
    12421 cgagtgacac ctaaatcttt atggggacgc aaaactaaag cttttccttg tttttctaat
    12481 tctgcaatct tatcccactg ttccttataa cgaatatgac gatttcttaa atcatccgta
    12541 atttgtttat ccttatatac caaattagat aaccacaatt gccattttgg tgctggatta
    12601 cgaacatagc cttcttcctt tgtagaaatc acaatatgct tatcatttcc agcacgaaga
    12661 ctttgttcaa tactaatcat atccacaaga cctgcatcca tccacatttc ccctttaaat
    12721 tcataaggtt tagctagaag taataatgca caacttgcct tgattaacgt ttcatcttta
    12781 tcaatatgac tcttatcaaa atattcaact ttatgcgtct ttaaattata tagacccact
    12841 tccatctgtg tttcgctatt ttgaaaagct tctaaatcta acggaactct agattcaata
    12901 aaatcataag ttgcttcaat accaaatata gagccttctt ttaaaaaggg tcttaaacca
    12961 actacacctt tttgacaagg cgcatcaatt ccagtcacct ctagacgatc cacttgttta
    13021 gacacataac tcaacaaaat ttctgtacct gcactaatac ctacacaata aggaagtaca
    13081 atatcatgct caagtaaaca cttcaatact cctgcagaat aagcagcctt agttcctcca
    13141 ccttctgcaa caatacctac tctactcacg cactacacca ctttcttttg ctgcatctgc
    13201 cactgcttgt gctacaacat ctgcaactct aggatctaga gctgaaacaa taatatgttc
    13261 actatctaac atatcttctg taaccattga actaatcgca taaacagccg cttctttcat
    13321 cgcgtcattg atacatgtag caccggcttt taatgctcct ttaaaaatac cagggaaagc
    13381 caatacatta ttgatttggt ttggatgatc actacgacct gtacccacga tataagctcc
    13441 agcttccttc gcatcatccg gttcaatttc aggaacagga tttgccatag ggaatacaat
    13501 tgctttttca ttcatagaag caatcatttc tttagatacg atgtgacctg cactaacacc
    13561 aataaataca tctgcaccaa ctaatgcatc ctttaaagaa cctgtttttc cttctttatt
    13621 taacttatca atcaatcctc tttgaccact tgttaaatcc atatccgccg ttaaaatacc
    13681 ttgtttatcg actaaaatca cattgcctaa tccaatcttt aaaattaaag ttgcaatcgc
    13741 aacaccagca ctacccgcac cattgataac gattgttgga ttttgttttt cagccaattt
    13801 taaagcattc actaaagccg cacaaaccac aatagctgta ccatgctgat catcatggaa
    13861 aacaggaata ttcatttttt cctgcaatct tttttcaact tcaaaacaac gaggagccgc
    13921 aatatcttct aagttgattc caccaaagct aggctctaaa cttgaaataa tttgaaccaa
    13981 ttcatctgta tcttgtgtat tcaaacataa tggaacggca tccacatttc ccaatgtctt
    14041 aaacaacaca catttacctt ccataacagg catacctgct tcaggaccaa tatttcctag
    14101 tcccaaaaca gcactaccat cactaataac ggcaaccgta tgagatcttc ctgtatatac
    14161 gtaactattc tttttatctt tttcaatttc taaacacggc gcagcaacac ctggtgtata
    14221 ggcaatagat aaatcatctt tgttttcaat cttcatttta ggaacagttt ccaatttacc
    14281 attccacttc gcatgtaatc ttaaagcttc ctttgcgtaa tccatgataa cctcctactc
    14341 aacaattttt aatacaattt caaccatctt ttccatcttt tgaatgcttg cgaattcaaa
    14401 acgaccatga tgattaaaag aacctgtacc taagttagga caaataagtc ctctagccgt
    14461 caacatagcc ccatcggttc caccacggat tggcgtacta acaggagtaa catcacattg
    14521 acgcaaagca tattttgcct tattcacact acgcatatct ccatgcatat attgacgcat
    14581 gttttcatat tgatcacgaa tcgttacatg aacacgatta ccgtactttt cattcatctt
    14641 atctttaaca gcttccatct tctgtttttg tgcaataaaa ctctgtttac tatgattacg
    14701 caaaatataa gttaattttg cctcttcaca aattccttcc ataccaacta aatgatagaa
    14761 accttgacga ccttgtgtat gtgaaggtgt ttgtttcttt ggaagacttg aagcatattc
    14821 catagctaat aaactcgcat tgaccatacg atcttttgca tctccaggat gaatactcgt
    14881 tccatcaaat gtcacaactg cttgtgctgc attaaatgtt tcataatcaa cagaatcaat
    14941 acgatctcca tcaattgtga acgcataatc cacggcaaac ttatctaatt caaagttttc
    15001 gacaccacga ccaacttctt catctggcgt aaaggcaacc gcaataaatc catgttcctt
    15061 ttcagattga atcaactgtt ccatagcctc taaaataatc gcaattcctg ctttatcatc
    15121 agcaccaagt aaagtcgtcc catctgtaac aatcaaatca tcaccaatac agtttgccaa
    15181 agctggaaat tcttctttac acattgaata ctcttcattt aattgaattg tatttccatc
    15241 atactttgaa atcacacgac acttcacatt cgcacccgta atttcactag ccgtatccat
    15301 atgcgcaata aagccaatac gatccatctt tttatctgta ttcgcattta agtaagcata
    15361 tacgattcct gtatccgtta attcaacaga atcaaaacct aatgattcac attctttttt
    15421 taattcacga gctaacacca actgtttttc agtactaggt gcacttgtac tttcttcact
    15481 tgactgtgtg tcaaatgatg cataatgcat taatctcttt tctactgcca tattcaatcc
    15541 cccttttatg ccagttcttg tgctaaacga ggcacaattt gttttttacg agaaacaaat
    15601 ccttgatctt taaattcttc cacaacatca cgaacaaaat aagataattt tcctacatat
    15661 aagaagcgtg atccacgacc ttccacattt gtgataacca tcaacattaa gtcatattca
    15721 tgaactttat tttgttcctc catatacttc aagaaatcct ctttgatttc atccacaata
    15781 ctcaaatcaa aagtgaaaac ttgacttacc gcaatgtgat atccactcaa catatattct
    15841 ttaacatcat tataaagaat gtttttgaat tcttttccac gaatcgtagc cgtattttca
    15901 aataattctt tagccatcgc atcaaaatca agatcatatt cacttgataa acgtttagct
    15961 aaagtatgat caatcgaagt acatgttggt gaattgaaat tcattgtatc actaatcatt
    16021 ccatacgcaa ttaattttgc gatagtttca ggcattttta caccatattc actatacatt
    16081 aatccaacaa tcgtgcatga annnnnnnnn nattggacga tctgtttcaa tatctccaat
    16141 tctatgatga tcaacaatct ctacaatgtc tgccgtttca atatcactga tactctgttt
    16201 cttttcatta tgatccacaa gaataaaacg tttcttttcc gccttcaata agttatagcg
    16261 agctactgaa cccaaaattt tattttggaa aacaaccgga taagaagagt gacgcgtttt
    16321 taacatttca cgctctacat cttccacata ctcactctta ttaaagcgaa tcactttatt
    16381 ggcaggagtc ataatcaatt caacaggaat ggaacgataa atcatttgaa taatcttcat
    16441 aatattataa ggtgtactaa tcaaagtaac tcccatctgt tttgccattt caataatata
    16501 actatttgga acaaagtttt ccgcaaccac aataaccgca cagccctttg ccatactctt
    16561 gatcatgcta tcttcatgat cattcaagac cataatagct ccttttaaat ctcgttccat
    16621 caagcgtttg tccgcaatac gaacaaatcc acttctttca agatttccct ttaaaataaa
    16681 agaccccttt acactggcca ctaaattttc taaaggcgtt tctttcaaca attcacttgt
    16741 aatatttaaa tcctgcattt gaaccttgga aacatctccc aaagtaacca tacctttata
    16801 ttcattttta tcatctacaa catataaagt cttaacatga ttttctaagc atagatccca
    16861 tgcacgacga attgtttgcc cctcttcaat tgtcaccgcc tcatcgattt caatttcctc
    16921 taacgttgat cgtgctgttg ttaataagac aggcatattc acatttaatt tgtctaatat
    16981 aaatttagtt tctggattca atgtgcccaa acgagcagct gttgcattaa ttcctgtctg
    17041 cctttttaat tcagcaagag ccaaagctgc acatatcgaa tctgtatctg gatttttgtg
    17101 tccaatcaca taaattggta agctcattaa aatctcctct caataatctt aattttgtac
    17161 tcaatattat atcacgaaaa aaaagaagat tcattatcga atcttcttac ttaacagcat
    17221 ctttcaaagc cttgctagct ttaaatttaa cagacttgct tgcagcaatt tcaatctttt
    17281 ctttagtagc tggattgata cctgtacgag cggctctttc actaattgag aatttaccaa
    17341 atccgaatac atctacagta ccattatctt ttaaagtatc agctactgta tcgaatacga
    17401 attgaacata agctgttgcg tctttttttg aacaaccaaa ttcttcagcc aacttttcag
    17461 ctaaatcttt tttattaata acattagaca tttatatgtt cctcctctaa attattacca
    17521 cgctatttta tcatttctct tactagagtg caacgtcttt catcatcgtt tcaacgacat
    17581 atttaggtaa accccgtctt tagaggtttg caccatgaat gtttagaggc tattctgaga
    17641 aaaagttgag attctccagt atttatcgtt gttttttgtg ctttaaattt att
//
LOCUS       DBMDWL010000021        17641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1084_length_17641_cov_2.574515, whole genome shotgun sequence.
ACCESSION   DBMDWL010000021 DBMDWL010000000
VERSION     DBMDWL010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 17641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17641
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1084_length_17641_cov_2.574515"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(773..862)
                     /locus_tag="ACZPDN_02645"
     tRNA            complement(773..862)
                     /locus_tag="ACZPDN_02645"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(823..825),aa:Tyr,seq:gta)
     gene            complement(1225..1296)
                     /locus_tag="ACZPDN_02650"
     tRNA            complement(1225..1296)
                     /locus_tag="ACZPDN_02650"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1261..1263),aa:Gln,seq:ttg)
     gene            complement(1302..1472)
                     /locus_tag="ACZPDN_02655"
     CDS             complement(1302..1472)
                     /locus_tag="ACZPDN_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297553.1"
                     /translation="MGNQDKHCYQCKHRHKLYCEKPCNACNGNPNVVKGKDNFTELET
                     ANKNAVLFETKE"
     gene            complement(1472..1888)
                     /locus_tag="ACZPDN_02660"
     CDS             complement(1472..1888)
                     /locus_tag="ACZPDN_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015758.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HIR1297554.1"
                     /translation="MQGNEYQKLAMRTNDKMAHHRLSTELTGKLPLSPLAESNAKCSN
                     INDIAGLLNGVLGLTGEAGEVSDLVKKGIFHEKGIDLEHLKKECGDVMWYVAMICEAC
                     GFSLDDVMQTNIDKLIARYPNGFDSYRANHRQAGDK"
     gene            complement(2407..3069)
                     /locus_tag="ACZPDN_02665"
     CDS             complement(2407..3069)
                     /locus_tag="ACZPDN_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297555.1"
                     /translation="MEKLQETPDTPEIFQNDIELYLTKFCEEHNIEDMTKEPQSRWNA
                     ALMYINKYVFSDKSILKLNKNINKNNTNCIMDSNFYMYDLDKLEYILYIYYYMCSMYD
                     KECSILGFSLLTGIHKDTFMDWGANERKLSTKGFELVQKLRDFREESLSNKLATGNKN
                     PVGILAILNRHFAWNLPGVSRESSNKTALTAAEIRQQLSQNNAQLTDKQQINAVNNSD
                     TI"
     gene            complement(3238..3723)
                     /locus_tag="ACZPDN_02670"
     CDS             complement(3238..3723)
                     /locus_tag="ACZPDN_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297556.1"
                     /translation="MEMTKEIREGKEIYAQRKNYENAELAVLNGATEEQAQAIARLCG
                     DRHYIHRNGSSVFHAESGDAETIGELLSNCSTGESINDYLSKVGLPRIEYTYSFDDDT
                     SNDYLYELEEMTYEEAEEKTEEVMNQFDEDIIKYIQDFDKKYNTHFTPTLAGRMKGYK
                     F"
     gene            complement(3746..4018)
                     /locus_tag="ACZPDN_02675"
     CDS             complement(3746..4018)
                     /locus_tag="ACZPDN_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297557.1"
                     /translation="MTINDKRFIIITEIKKGQPKGWKVKRMKTIELLNKAVELGFSRE
                     KALADIDASLDEIIGAENRKPITEEEVSEELASDILFGFECEKENN"
     gene            complement(4042..4248)
                     /locus_tag="ACZPDN_02680"
     CDS             complement(4042..4248)
                     /locus_tag="ACZPDN_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297558.1"
                     /translation="MLKYKIDVLETLKESGYNSTRILKENLISQSAVQKIRKNEMVGI
                     KTIEKLCELLDMQPGNIIKYVEKK"
     gene            4490..4870
                     /locus_tag="ACZPDN_02685"
     CDS             4490..4870
                     /locus_tag="ACZPDN_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297559.1"
                     /translation="MRFEVKDDTITSETLEKTDIYKIVEKIPFGFFVWNIGENMGSDE
                     YVPLCQYLSPGIKDDYSINPDTLRAIKLPKEDVELLREAAGWGVNSLETARKALKSRR
                     HSYTAEKKRENARKTIDIFERITE"
     gene            4883..5053
                     /locus_tag="ACZPDN_02690"
     CDS             4883..5053
                     /locus_tag="ACZPDN_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297560.1"
                     /translation="MNWQVIETQYFSKFESPKEKIVAQFDTLVLAEDFVNLVIPKDTR
                     DRFKIEHINKEV"
     gene            5057..5314
                     /locus_tag="ACZPDN_02695"
     CDS             5057..5314
                     /locus_tag="ACZPDN_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297561.1"
                     /translation="MKILLEKIKKLEQLEKAADEAESRYTEEPENAEFESAFDKAYKA
                     EFAAYIDVARYIEHMTGGAIDFMKAKELIQTKRAELLQLLA"
     gene            5492..6214
                     /locus_tag="ACZPDN_02700"
     CDS             5492..6214
                     /locus_tag="ACZPDN_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297562.1"
                     /translation="MAGYYKNQMSNNAVWAYSQGEKPMYKWTKTTILEEIDNIFWHAD
                     KKTEIDFKKMTLKELKENFLVWSSWHHTGKIYNETDFYCIEENAVLNFTVKEFDEIIS
                     NRKKRTYTRRTAAELEQIKAEKEKDILLTERSKEIYRKLYIIYIYKSDLKTFKGLINR
                     FLNDKINIEKDFAESVEIARQKEEHRIKCWQGDVNDWHNREGIVDLYYKDISTYVLKM
                     RGVENYQIDKKLLKQIKNKIVN"
     gene            complement(6211..6456)
                     /locus_tag="ACZPDN_02705"
     CDS             complement(6211..6456)
                     /locus_tag="ACZPDN_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297563.1"
                     /translation="MPLQVVEAVASFGGFSRTHAAQLGTLVHLKSLIYKHSIIVLIFQ
                     GTQRKAYKYIRLVLAAGIEPAKPEQAKKGADYTPLKS"
     gene            complement(6908..7606)
                     /locus_tag="ACZPDN_02710"
     CDS             complement(6908..7606)
                     /locus_tag="ACZPDN_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297564.1"
                     /translation="MRVIRLKMYQEMARFNNPSAPRGADCYPLPPFSTVNGFIHSMCQ
                     WKRYHKLDYFVTGKGVYNTKTQKEWHGGKRFNKVSDEMLKRWDVITDYADGSHTGWVS
                     TVKYHLMLVDLYTTIYIKADDSDIDDIYNALLNPPVYPSLGEYGDLCKIEAVDIIELK
                     ELGEPVSVPLDMQSYIPVNKGNFAGTIYRINNKYEIVKGLRRFQKVSCYLVDKGQEVV
                     SNLFDDDKPIIFID"
     gene            complement(7738..7884)
                     /locus_tag="ACZPDN_02715"
     CDS             complement(7738..7884)
                     /locus_tag="ACZPDN_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297565.1"
                     /translation="MSIDELLKTVQKTNPTMTKELLIYELSQCQYSSKALIYTEKCCI
                     DSNA"
     gene            complement(7884..8204)
                     /locus_tag="ACZPDN_02720"
     CDS             complement(7884..8204)
                     /locus_tag="ACZPDN_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR1297566.1"
                     /translation="MIFNINGTMWQVQYKNSNSSELKRSDGTISLGVTDRNTHTIYLS
                     NALRGFMQRKVLIHEVCHAICMSYDVYLPIEQEEILCDFVATYGDEVFDIVDMVLGAV
                     RRVG"
     gene            complement(8264..8530)
                     /locus_tag="ACZPDN_02725"
     CDS             complement(8264..8530)
                     /locus_tag="ACZPDN_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297567.1"
                     /translation="MRDIDRDYGKMYYTEPMSESNYDRAKRNYTETKEIHKNNTPEDK
                     EHKMKALDGYIKELGGDITQLIGDMTAEERNLMRTKLSTLVSKL"
     gene            complement(8527..8898)
                     /locus_tag="ACZPDN_02730"
     CDS             complement(8527..8898)
                     /locus_tag="ACZPDN_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297568.1"
                     /translation="MHIERMHKMQECLTEKAVSEFEKGIENVDTSEMGEVVDMIKDLA
                     EAEYYSIISKAMKKADEEEEEYDKELLRSLKAEYGEKVVEDITTTTAMLMADSPQKVK
                     EHTAGDMKSRHITICTQKQSI"
     gene            complement(8910..9329)
                     /locus_tag="ACZPDN_02735"
     CDS             complement(8910..9329)
                     /locus_tag="ACZPDN_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297569.1"
                     /translation="MEITGIALQTVAAGEDVAFTETPVCGSKCIVHRQGSGIIKLRGI
                     TNQCKARFLVSYSGNIQIPTGGTVGAISLAIAVDGEPLQSTRMIVTPAAVQNLFNVSA
                     QAYVDVPCGCCSTVAVQNTSTQAIEVQNSNLIAVREA"
     gene            complement(9469..10239)
                     /locus_tag="ACZPDN_02740"
     CDS             complement(9469..10239)
                     /locus_tag="ACZPDN_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297570.1"
                     /translation="MFNSNGVSLADIAAVTGNNRNNDGMWGDGAWWIVILLIFGWGNN
                     GWGGFGGNGNGAGYTDSAIQRGFDNQAVISKLDGISNGLCDGFYAMNNSMLTGFNGIN
                     TNIMQTGYGIQQAINADTVANMQNINALQAQLANCCCETREAIQGVNYNMATNTCALQ
                     NTMNNNTRDIIDSQNAGTRAILDYLCQDKIATLTAENNDLRRAASQDRQSALLTTQMA
                     AQTQQIINAVNPAPIPAYTVPNPNAYAYGCGCNTGCGC"
     gene            complement(10311..10538)
                     /locus_tag="ACZPDN_02745"
     CDS             complement(10311..10538)
                     /locus_tag="ACZPDN_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297571.1"
                     /translation="MNQQLIQTINQLKSIRNPQQMAMNCLQQSAQRGNPMAKNLLNQI
                     NSGNTQGAEQILSNFMNTQGINLNDIKGMMN"
     gene            complement(10535..11065)
                     /locus_tag="ACZPDN_02750"
     CDS             complement(10535..11065)
                     /locus_tag="ACZPDN_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297572.1"
                     /translation="MNNPYQAVPMMNNSYMQSQNPYMDRMNFLQNYQQSLQQQPMQMN
                     QQPMPQQIAGINGRIVQTVENINANEVPMDGSMAFFPKQDMSEIYVKGWNADGTIKTV
                     VYKPYTAPKDNQTVNSMANTENAKFTLSDESTQLFLNKFEELSEKIGQLENRFDKSLG
                     TQRKTSRTQSKGGDEE"
     gene            complement(11028..11183)
                     /locus_tag="ACZPDN_02755"
     CDS             complement(11028..11183)
                     /locus_tag="ACZPDN_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297573.1"
                     /translation="MFSDEVREKILSKEELQKLDLVTLSLVIHAIEEVLEEADNEQSL
                     SSSAYDE"
     gene            complement(11561..12748)
                     /locus_tag="ACZPDN_02760"
     CDS             complement(11561..12748)
                     /locus_tag="ACZPDN_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1297574.1"
                     /translation="MGKDLKGKELGVGLSQRKDGVYQGRYKDRFNKIKYIYGTKLSEV
                     KKELAVAIAENIQFTSIRDDIKLDDWFNRWIEVYKKKSVRPNTLREYTHIYNKNISPF
                     LGNRNINSFVKSDIQTLIDKIADDNYKYERQNKIKVILNDMFSRAIEDDLMIKNPAKG
                     VKLRADKELKAFTLTAKQQIEFLEASKGTFYDNLYNVAVNTGLRPGELFALTPNDIHL
                     DEGYIDVNKTLVYQKYLDDKCKTFHIEPPKTKQSYRQVPINSECIKYLEKQFELKDIV
                     KCKRPKEQNNYLFVTSYNTPLNSQIYSDSIKAIVKQINLARSFDNEFPVFSGHTLRHT
                     FATRCFEAGVQAKVVQSYLGHATLKMTMDLYTHVTEERAAVDIERIVKDKDNIVDFKK
                     SAV"
     gene            complement(12831..13229)
                     /locus_tag="ACZPDN_02765"
     CDS             complement(12831..13229)
                     /locus_tag="ACZPDN_02765"
                     /inference="COORDINATES: protein motif:HMM:NF020602.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HIR1297575.1"
                     /translation="MKFSGLTKPELDEIIENANFTDEELRIFKLLVGNMSLEQVSQRL
                     MLSKATISRRVKDIKIKIERTDDMVKTIPIWEKVTLTVEEASEYSNIGINRISSMLNE
                     ISCPFVLRVGNKRLVKRKEFEHYIEKSNEI"
     gene            complement(13242..13520)
                     /locus_tag="ACZPDN_02770"
     CDS             complement(13242..13520)
                     /locus_tag="ACZPDN_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297576.1"
                     /translation="MSKAEQKKFKEQMLRVQMNRISNEQQKKNFESALILIMWVLHDK
                     FGFGQQRLTKVQRELKALIDNYNDGLFTAEELVNQLYEETGIEHIKFK"
     gene            complement(13525..14169)
                     /locus_tag="ACZPDN_02775"
     CDS             complement(13525..14169)
                     /locus_tag="ACZPDN_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297577.1"
                     /translation="METTPISPQKKLYNYRRENGLCPKCGKPLDRKGFYCEECRKKQT
                     TYSRETRELCRQFKICPECRKNKLVGDEKICPECLANKAEYRANHPLSDDKRRKNNEA
                     FKQYSKNLYAERRKAGICVRCGKAKAVEGKAKCFICQSKDNAIHRKRTENRQNIKEYR
                     KENHLCYRCGEPIDRPQGQLCQKCWQTDYERGKSLKNDNSKHYWRYDNQFLRKK"
     gene            complement(14176..14838)
                     /locus_tag="ACZPDN_02780"
     CDS             complement(14176..14838)
                     /locus_tag="ACZPDN_02780"
                     /inference="COORDINATES: protein motif:HMM:NF022858.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative helicase loader/inhibitor"
                     /protein_id="HIR1297578.1"
                     /translation="MTREQVGKLLMTIQAYYPNYNPPDKEITLNAWHIMLAEYPEELV
                     LQALRACITTNTSGFAPDVGQIMSKIQTISQPQELDGMAAWGLVSKALRNGTYGAVEE
                     FNKLPPLVKQAVGMPDNLKNWATSDYQTIETVIQSNFLRTYETVVKRVNEINRMPDNI
                     KSLIKKANANSYKAQIEQKFQRDINTLQIKENALISKNTNAEEYIEAPKEIQDRIDRM
                     RG"
     gene            complement(14835..15620)
                     /locus_tag="ACZPDN_02785"
     CDS             complement(14835..15620)
                     /locus_tag="ACZPDN_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297579.1"
                     /translation="MAELEEVIMAGNFIKIDRKILKWEWWSDINTFRLFMYMLISAYW
                     KDGNYKGKIIERGSFPSSISELSKETNLSVMEIRTSLKHLQLTGEITSKATNKFTIFT
                     VVNYNLYQTDNKQDNKQITSNLTNNQQTDNILLTNSILKESKNERTEEIKEDKNIEKD
                     ITNVISKKKSYYPDDELLDEAFNEYVTMRKRIKKPICTDKALHRAMNTLEKLSGGDND
                     LAIKILNQSVDHCWQGLFELKEDNSNKQQGKKNVFDEWMEAMK"
     gene            complement(15625..15816)
                     /locus_tag="ACZPDN_02790"
     CDS             complement(15625..15816)
                     /locus_tag="ACZPDN_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297580.1"
                     /translation="MKDETKQEIQILLDLLKGSLTRNGVSMAADNSGNLMFFDTTAYI
                     KSKGKEFDGFRVNINDLVK"
     gene            complement(15816..16532)
                     /locus_tag="ACZPDN_02795"
     CDS             complement(15816..16532)
                     /locus_tag="ACZPDN_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297581.1"
                     /translation="MIVHCLFEQSGTFKNAFKKYGIEAYDYDIQNEFNETDYVTDLFK
                     EIEGGYQGEPSLFDKISPDDLILAFFPCTYFSDQSPRHLCCTAYQYKNYTIEQKCEVS
                     MKRHRQLSLFYEVLNKFVIVCQRKHLRLIIENPLSTSGMHYLTHFWCLKPNVIDKDRT
                     LNGDYYKKPTQYWFIGLQPKNNFIFEPLEAVDVMKQRYVTSDNPLGVDRKTARSMIHP
                     QYADRFIRQYILDEEIWRGK"
     gene            complement(16604..16777)
                     /locus_tag="ACZPDN_02800"
     CDS             complement(16604..16777)
                     /locus_tag="ACZPDN_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297582.1"
                     /translation="MSNDANIVISQALMMRIKDYAERALDRKDVTVDIAMSEIRYTVD
                     AYDEYFQTGRKPQ"
     gene            complement(16774..17373)
                     /locus_tag="ACZPDN_02805"
     CDS             complement(16774..17373)
                     /locus_tag="ACZPDN_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR1297583.1"
                     /translation="MNYQNIARVKAIEQENKKRLLKLNPKLNDRSGIYFLLREDENGF
                     KYAYIGQAVHTLSRLASHLVGYEQHIDLSLRKHKLYDKEKNPYGWRVEFLNFPESQLD
                     EKEKYYIKLYADKGYQLRNVSLGGQGENRASGSIGERKAPKGYMQGVQQGKKVLAREL
                     SSIAEKHLKIEIRDDKKHNKVSQKQYEKFMDLLKVGDSE"
     gene            complement(17370..17552)
                     /locus_tag="ACZPDN_02810"
     CDS             complement(17370..17552)
                     /locus_tag="ACZPDN_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297584.1"
                     /translation="MRLIDADKLIEDIHKRNYIDKALSEIFETIINEQPTAFSMAAKP
                     IDNFVNPFEVKAGDNS"
BASE COUNT         5058 a         3528 c         2746 g         6309 t
ORIGIN      
        1 cttgtgctta atctgtgaat aacaaaaaca acaaacaggc agttcacagg tctgcttttc
       61 gcttcgtcaa gctagtttgt tgtaatcgga tagacagggc ttgaacctgt gactacttga
      121 ataaatcaag cgttactccc atctgaacta ctatccgttg tgcagtttct tgtgttggaa
      181 agtatttatg gcacttcatt acgctatctg ccatcctgtt cgcaaatcaa ccaacacaag
      241 cattttaatt attcagcagg gattactgca acgcctgctt attcgggagc tacccgacta
      301 cttgatgtgg tgtggatttg aaccacacat aaacaagcac tcctgtcctt tcaagcccct
      361 agcaatcagg tattcccctg tggttatgct atggtagatt cgaaccacta gctcattcta
      421 tctgctatta gcgtttaccc attccgccac acatcaactt actcacacct cttaacctag
      481 gataagtctg caaacaacat tacgcacgca gacctaagaa gtgctttcaa aacgccgata
      541 tcgtgaatcg aacacgaaca acatttctgt tggatagctt agcaagctac tggaatacct
      601 ttatcccata tcggcaagcg gtggtttttt acttggttat caccacccaa ggatttttta
      661 gtcagccgca agcggctcaa tccagttccc tgtactaagt ttaaccggta tattgattag
      721 cacctgcatt tctctaataa acgcactagg ggtgtactgg caacatcgcc aatggggaag
      781 agaggaatcg aacctctatt gtttaccact tgggaacaga tttacagtct gccgcaacac
      841 cgccaatcgt tgccgcttct ccatatcgtt ttaaaagact agcattgtga aaatgtttcg
      901 attaaggtgg atagttgata ctgaaaaaca atgctagtct taatagcagt ataggctatg
      961 acacctataa caggtcgtgg caaagcttgg atgtcattct acccgtgcag ttgggcttaa
     1021 agaaagtagc ttcgctcgct gtctatccat acagataact gctgcgctat aggtataact
     1081 taattttatt tgcgtattta tatacgcaaa acctcacgga ctatctgaca gtccttaaca
     1141 gctctcgcta tgaggtgaaa ggaggactta atgctagtaa accaataagt cctgtaaagg
     1201 cacaagtgta attaaacact tgaactaccc ctgtgggatt tgaacccacg atacaggaat
     1261 caaaatcctg tgccttgacc acttggctaa ggggcaatat gctattcttt tgtttcaaag
     1321 agtactgcat ttttatttgc tgtttcaagc tctgtgaagt tatccttgcc ttttacaaca
     1381 tttggattgc cattacaggc attacaaggc ttttcacaat ataacttatg tctatgtttg
     1441 cactggtaac agtgcttatc ctgattaccc attatttatc acctgcctgt ctgtgattag
     1501 ctctgtaaga atcaaagcca ttcggataac gtgctataag cttatctatg tttgtctgca
     1561 ttacatcatc aagactgaat ccgcaagctt cgcaaatcat agcaacgtac cacattacat
     1621 cgccgcactc tttcttgagg tgctctaagt ctatgccttt ttcgtggaat atgccctttt
     1681 taacgaggtc tgatacttcg ccagcttcac cagttaaacc taagacgcca ttaagaagtc
     1741 ctgctatgtc atttatgttg ctacacttag cattgctttc tgccagagga ctaagtggaa
     1801 gcttaccagt taattcggta cttaatctat gatgagccat tttatcgtta gtacgcatag
     1861 ccaatttttg gtattcattg ccctgcattt ataactccta actcttttct gttttttaaa
     1921 attttttgga atttattcag ccgactagct gattctctga tgtgtttatt gaatatcttg
     1981 tgattaatta atatgtgtct attatacacc taattagctt aaatgtatag atgttaattg
     2041 gattattttt aattaaatat ataagtgatt tattagtatt aattatatga ttaatggtta
     2101 ggtgctgttt atatataatt atataatatg tgtattatgt gggtaataat aatataaata
     2161 tatattaata tataaagcct ttttcttatc gtggaaaaat gagtgactta gttggggcgt
     2221 gttccgagga caaataaacc ccctccgccc ttgtccgtgt aattgtgtct attttacgcc
     2281 atattctcaa acaattaaca caattaacac catatccata ctataacgcc gataaacctt
     2341 aatttatcag cgttatataa atacttatta ctcacaaacc cagtatttaa gcggtttgca
     2401 agctgtttaa attgtgtctg aattgtttac ggcgtttatc tgctgtttat ctgttaattg
     2461 cgcattgttt tggctcaatt gttgacgtat ttccgcagct gttagagctg ttttgttgct
     2521 gctttcacga ctgacaccgg gcaagttcca agcaaaatgt ctgttgagta ttgcaagaat
     2581 tccaaccggg tttttattgc cggttgcaag cttattagat aaactttctt ctctaaaatc
     2641 gcgcagtttt tgaaccaatt cgaagccctt tgtacttagc tttctttcat tcgctcccca
     2701 atccataaaa gtatctttat gtattccagt taataagcta aagcctaata tactacattc
     2761 tttatcatac atagaacaca tataataata tatatataat atatactcta atttatctaa
     2821 atcatacata taaaaattac tatccattat gcaattggta ttatttttat taatattctt
     2881 atttaacttt aatatacttt tatcactaaa aacatattta tttatataca ttaatgcagc
     2941 attccatctg ctctgtggtt ctttggtcat atcttcgatg ttgtgctctt cacaaaattt
     3001 tgtcagatat aattctatgt cattctggaa tatctccggt gtgtctggtg tttcctgtag
     3061 tttctccata tattcccctt tccgctggac ctgctccagc taattaatta ttatacattt
     3121 aataacataa aaataaccca ataatatttt taatattatc gggtgtaaat cttatatatt
     3181 taattattaa aataatatag cataaatata ttataaagtc aatcttcttc tatcgtctta
     3241 aaatttgtat cccttcattc ttcccgctaa agtaggggta aaatgtgtat tatatttttt
     3301 gtcaaaatct tgaatatatt ttattatatc ctcgtcaaat tggttcataa cttcttcggt
     3361 tttttcctcg gcttcctcgt atgtcatttc ctctaactcg taaagataat cgtttgatgt
     3421 atcatcatca aaactgtaag tgtattctat cctcggcagt cctaccttgc tcaaatagtc
     3481 attaatgctt tctcctgttg agcaattact tagcaactcc ccaatcgtct cggcatcacc
     3541 ggactcagcg tgaaaaacac tgcttccgtt cctgtggata tagtgtctat ctccgcacaa
     3601 tcgcgctatt gcttgtgcct gttcttctgt agcaccatta agcacagcta attcagcatt
     3661 ttcatagttc tttcgctgcg cgtaaatctc tttgccttcg cgtatttctt ttgtcatttc
     3721 catattattc accttttaac ctttcttaat tgttttcttt ttcgcattca aacccgaata
     3781 aaatatcgct tgccagctct tcgctgactt cctcttctgt aattggcttt ctgttttctg
     3841 ctccgattat ttcatcaaga cttgcgtcta tgtctgcaag cgccttttct ctgctaaatc
     3901 caagctcaac agccttgttt aataattcga ttgttttcat ccttttcacc ttccagcctt
     3961 tcggctgtcc tttcttaatt tctgtaatta taataaacct tttatcgttt attgtcaata
     4021 cttttttaaa tcttttttag tttatttctt ctctacatat ttaatgatgt ttcccggttg
     4081 catatccagc aactcacata atttttcaat cgtcttaatc cctaccattt catttttgcg
     4141 gattttttgc accgctgatt ggctaattaa gttttctttt agtattcgcg tagaattgta
     4201 gccgctctct tttagtgtct ctaaaacatc tattttatac ttaagcattg tttgttttac
     4261 ctccttttat ttttattaca ttatataaaa tacattctca aaagtcaatc aaaaaataat
     4321 ctaaaaaaag tttattttat ctattgactt taaactgttt taggtttatt ataataactg
     4381 tcaagaggac atacaaaaag gcggtcactc ctaccaagaa cgaaccgcca ccaatcaaaa
     4441 aaagaaaggt aagccgatta tatcacagtc ggcgaaatgg tacaagatta tgagatttga
     4501 agttaaggat gacacaatta caagtgaaac attagagaaa acagatattt ataagatagt
     4561 cgaaaagatt ccattcggtt tctttgtatg gaatatcggc gagaatatgg ggagcgatga
     4621 atatgtccca ctttgtcaat atttgtctcc aggaattaaa gatgattact ctatcaatcc
     4681 cgacacttta agagccatta aactgccaaa agaagatgtt gagttattaa gagaagccgc
     4741 aggctgggga gttaatagct tagagacagc aagaaaggca ttaaagagcc gccgccatag
     4801 ttatacggct gaaaagaaga gagaaaacgc acgcaaaaca atagatatat ttgaaagaat
     4861 tacagaataa ggaggattaa atatgaattg gcaagttata gagacacaat atttcagtaa
     4921 atttgaaagt cctaaagaaa aaattgtggc acaatttgat accttagttt tagcggagga
     4981 ttttgttaat ttagttattc caaaagatac acgcgacaga ttcaagattg aacatattaa
     5041 caaggaggtg taaattatga agattttact tgaaaagatt aagaagttgg aacagttgga
     5101 gaaagccgca gacgaagcag aaagcagata tacagaagag ccagaaaacg cagaatttga
     5161 aagtgccttt gataaagcat ataaagcaga atttgccgca tatatcgacg tggcaagata
     5221 tattgaacat atgacaggcg gtgcgattga ttttatgaaa gcaaaggaat taatacagac
     5281 taaacgcgcg gagcttttgc agctcttagc ataatttgca aggttgacac ttccagggtt
     5341 cgattccccg gcttgctaaa ataaaagaga ggaagtacaa tatacttcct ctcttaccat
     5401 tcaaaatagc acttgtccta aaaatataca acaacaaatg tctttgttat aatagcatat
     5461 aaaatataaa aagtaaagga gatttaaaaa gatggcagga tattataaaa atcaaatgag
     5521 taacaacgcc gtttgggcgt attcgcaagg tgaaaaacct atgtataagt ggactaaaac
     5581 cactatttta gaagagatag ataacatttt ttggcacgct gataaaaaaa cagaaataga
     5641 ttttaaaaaa atgacattaa aagaattgaa agagaatttt ctggtgtggt cttcctggca
     5701 tcatacggga aaaatttaca atgaaacaga tttttattgt atagaagaaa atgcagtatt
     5761 aaactttacg gttaaagaat tcgatgaaat tatatctaac agaaaaaaga gaacgtatac
     5821 aagaagaact gcggcagagt tagaacagat taaagcagaa aaagaaaaag atatattgct
     5881 taccgagaga agcaaagaaa tttatagaaa attatatata atttatatat ataaatcaga
     5941 tttaaaaacc tttaagggtt taataaacag gtttttgaat gataaaataa atatagaaaa
     6001 agattttgcc gaaagtgtag aaatcgcaag gcaaaaagaa gagcacagaa taaaatgctg
     6061 gcaaggagat gtaaacgact ggcacaacag agaaggaatt gttgatttgt attataaaga
     6121 cataagtacc tatgttttaa aaatgcgagg agtggaaaat tatcaaattg ataaaaagct
     6181 tttaaaacaa ataaaaaaca aaatagtgaa ttagcttttt aagggcgtat aatctgcgcc
     6241 ctttttggct tgctctggtt tggctggttc gattccagct gcaagcacta agcgtatata
     6301 tttatatgct tttctttgcg taccttgaaa aattaataca ataatgctat gcttatatat
     6361 aaggcttttt aagtgtacaa gtgtacctag ttgggcggcg tgcgttctgg aaaatccgcc
     6421 aaaactggcg acagcttcca caacttgtaa gggcatatta tacccatttt atacaacgct
     6481 gttaaagacg ttttaaggcg tgcaggtgtt aacccttgca attatgcact tgtaaaataa
     6541 aaccgccata caagttaaac cacaaagcca cgacgccaaa ataaacacga accgcaaccg
     6601 gtcaagtcta tatataatgc tttaccatac taaagttttt catcaatttt tcaaggcaaa
     6661 tctgaacaaa attgaggtca aatttgggga aaagttttca accgattttt ggatttcaaa
     6721 atcacatatg acgggggttt caaaaatttc acattatatt ttatgagaaa attttttaaa
     6781 tttttaaagt ataatttgaa cgaaatctga accaaatttt gagatttttt aaaattgaaa
     6841 ttgtgaatac aaaaagtcaa cccacggagg tagcaaaaaa gttgcattat attccgtggg
     6901 gtttaaatta atctataaaa ataatcggct tatcatcatc aaaaagatta ctaacaactt
     6961 cctgtccttt atccactaag taacaagaaa ctttctggaa tcgcctaagc cctttgacaa
     7021 tttcatattt gttattaatt ctatatatag ttcctgcaaa attgccttta ttaacaggaa
     7081 tataagattg catatcaagt ggaactgata caggttcacc aagttcctta agctctataa
     7141 tgtctactgc ttcaatctta cataaatcac catactcacc taatgatgga tatactggcg
     7201 ggtttagtaa cgcattgtat atatcatcta tgtcactgtc atcagctttg atgtatatag
     7261 ttgtatataa atcaactagc attagatgat atttaactgt gctaacccat ccggtgtggc
     7321 ttccgtctgc ataatctgtt ataacatccc aacgcttaag catttcatcg ctaactttgt
     7381 taaaacgctt gccaccgtgc cattctttct gcgttttggt attgtaaaca cccttgccag
     7441 taacaaaata atctaattta tgataccttt tccattgaca cattgaatga ataaatccat
     7501 taactgtgct gaacggcggc aaaggatagc aatccgcacc tcttggcgct gatggattgt
     7561 taaatctagc catttcttga tacattttta accttataac tctcataata aaacctccaa
     7621 aataaaataa gttgcaccta tacaaaaatg tatcaatgca actttccact atggttctat
     7681 taaggtaaaa tgatataata gttatctatt gtttacatct attaaataat agcattttta
     7741 agcattactg tcaatacaac atttttctgt ataaatcaat gctttacttg aatactggca
     7801 ttggctgagc tcatatatca ataattcttt tgtcatagtc ggattagtct tttgaactgt
     7861 ctttaataac tcatcaatac tcattatccc actctcctaa ctgcccctaa aaccatatca
     7921 acaatatcaa atacttcatc cccatatgtt gccacaaaat cacacaatat ttcttcctgt
     7981 tcgataggca aatacacatc ataggacata cagattgcgt ggcatacttc gtgtatcagc
     8041 actttgcgtt gcataaatcc acgcaaggca tttgacagat aaattgtatg tgtatttcta
     8101 tcagttacac ctaagctgat tgtgccgtct gaccgcttca attcacttga atttgaattt
     8161 ttatattgca cttgccacat tgtgccatta atattaaaaa tcatctgtat gctcctttct
     8221 gaataaaaca ggctatgaat attgctactc atagccctta aatttacagc ttagaaacaa
     8281 gtgtactaag tttggtacgc ataagattgc gttcctctgc cgtcatatcg ccgataagct
     8341 gtgtaatatc gccgccaagc tccttaatat atccgtcaag tgctttcatc ttatgttcct
     8401 tgtcctctgg tgtgttattc ttgtgtattt ccttagtttc tgtgtagttt ctctttgctc
     8461 tgtcgtaatt actttcagac attggctctg tatagtacat cttgccataa tctctatcaa
     8521 tatccctcat atgctctgct tctgggtaca tatggtaata tggcggctct tcatatcccc
     8581 tgcggtatgt tcctttacct tttggggcga atctgccatt agcatagcgg tagtggtcgt
     8641 aatatcttct accactttct cgccatattc tgccttaaga cttcttagga gttctttgtc
     8701 gtactcttct tcctcttcat cagccttttt catagctttg gaaattattg aataatactc
     8761 agcttctgca aggtctttta tcatatccac gacctcaccc atttcagaag tgtcaacatt
     8821 ttcaatgccc ttttcaaact cgcttacagc tttctctgta aggcactctt gcattttgtg
     8881 cattctttca atgtgcatac tctcaccccc tacgcttcac gaacagcaat taagttacta
     8941 ttctgtactt cgatagcctg tgtcgatgta ttctgcaccg ctacagtact gcaacagcca
     9001 caaggtacat ccacgtatgc ttgagctgaa acgttaaata aattttgtac tgctgccgga
     9061 gtaactatca ttcgtgttga ctgtaaaggc tctccgtcta ctgcaatggc aagtgatata
     9121 gctccaactg tcccgcctgt aggtatctga atgtttccac tataagatac taaaaatcta
     9181 gccttgcact gatttgtaat acctcttaac ttgataattc cgcttccttg tctgtggact
     9241 atacatttgc taccgcatac cggtgtttct gtaaatgcaa catcttctcc ggcggcaact
     9301 gtttgtaaag caattcctgt tatttccatt atctttacct ctctttcata aaaataaggg
     9361 caaacattat agtctgccct ttggttataa gtaatactgc ttagcagaca taatcgagtt
     9421 aaactcaatt aagatactca attattcatt tttgcgtagc tgctactttt agcagccaca
     9481 tcctgtattg caaccacatc cataagcata agcattagga ttaggcactg tgtatgccgg
     9541 gattggtgcc gggtttacag cattgataat ctgctgtgtc tgagctgcca tctgagttgt
     9601 aagtaatgca ctctgacgat cctgcgaagc ggctctgcgt aaatcattgt tctctgctgt
     9661 aagtgttgct atcttatcct ggcataagta gtcaagaatt gctcttgttc ctgcattctg
     9721 actgtcgata atatctcttg tattattgtt cattgtgttt tgtaaagcgc aagtgttagt
     9781 tgccatgttg tagtttacac cttggatagc ttcacgagtt tcacagcagc agttagcaag
     9841 ctgtgcctgt aatgcgttta tattctgcat attagcgact gtatcagcgt taatagcctg
     9901 ctggatgcca tagcctgtct gcataatatt tgtgttaatg ccattgaaac ctgtgagcat
     9961 actgttgttc atagcataga aaccatcgca aagtccgttg gaaatgccat ctaacttgct
    10021 gataactgcc tgattgtcaa aacctctttg tatagctgaa tcagtgtagc ctgcgccgtt
    10081 gccatttcca ccgaaaccgc cccagccgtt attgccccag ccaaagatta agagaattac
    10141 aatccaccat gcaccatcgc cccacatacc atcgttatta cgattattgc ctgttactgc
    10201 ggcaatatct gcgagactaa ctccgtttga attaaacatc ttgtttacct ccatttattt
    10261 tattaacaaa tgggataacc ggtcattatg tgcgcacaac ccaaaatgtc ctaattcatc
    10321 atacccttaa tatcattaag gtttattcct tgtgtattca taaaattact taaaatttgc
    10381 tctgcgcctt gcgtgtttcc actgtttatc tgattaagca agttttttgc cataggattt
    10441 ccacgctgtg ccgactgttg taaacaattc attgccattt gctgtggatt ccgaattgac
    10501 ttaagttgat ttatagtttg aattaactgc tgattcattc ttcatcaccg cccttacttt
    10561 gagttcttga tgtttttctc tgtgttccta aagatttatc aaatctattt tccaactgcc
    10621 ctattttctc cgataattcc tcaaacttat tcagaaatag ctgtgtgctt tcgtctgata
    10681 gggtaaattt agcgttttct gtattagcca tagaatttac tgtctgatta tctttagggg
    10741 ctgtataagg cttatacaca accgtcttaa ttgttccgtc agcattccaa cccttaacat
    10801 atatttccga catatcctgt tttgggaaaa atgccattga gccatccata ggcacttcat
    10861 ttgcattaat attttcaact gtctgtacta ttcttccgtt aatgcctgct atctgctgtg
    10921 gcataggctg ctgattcatc tgcataggct gttgttgcaa gctctgctga taattttgca
    10981 aaaagttcat tctatccata tatggatttt gagattgcat ataagaatta ttcatcatag
    11041 gcactgcttg ataaggattg ttcattgtct gcctcctcta aaacttcctc gattgcgtgg
    11101 ataacaagag ataatgtcac taagtcaagc ttttgtaatt cttctttact caagattttt
    11161 tctctaactt catctgaaaa cattcgcact acctctcttt ctgattatat ttttgcataa
    11221 aaaaagagaa gaacattatc aagttcttct catatttatg tcatacatca aggctttatt
    11281 tagttttaat ttactacaca cttttaatct tgttactaca cacttactac acacttttgc
    11341 tattgaaata tatagaaata catagaaata tgtggaaatt gataattaat ctaatgccgc
    11401 ttaaaatccc ttaaataccg catttattgt gctttttgtt aagttcataa ggggtagtct
    11461 gatatacata ataagttttc caagttaaat atattataca tcttaaaccc ccttattttg
    11521 tggttttttc agttttaatg tgtgtagtac tacacactta ctacacagca ctttttttaa
    11581 aatcaacaat gttgtccttg tctttcacaa ttctttcaat atctactgcc gctctttctt
    11641 ctgttacgtg tgtatataaa tccattgtca ttttaagagt tgcatgacct aaatatgatt
    11701 gaacaacttt tgcctgcaca cctgcttcaa aacatcttgt agcaaaagta tgtcttaaag
    11761 tatgtccgct aaacacagga aattcgttat caaaacttct tgcaagattt atctgcttaa
    11821 caatagcttt aattgaatct gaataaatct gcgaattgag aggcgtgtta taacttgtca
    11881 caaacaaata attgttctgc tctttaggtc ttttgcactt tacaatatcc tttaattcga
    11941 actgcttttc aagatattta atgcattcgc tgttaatagg tacttgtcta taactctgtt
    12001 tggttttagg cggctcaatg tgaaaagttt tgcacttatc atcaaggtat ttttgataca
    12061 caagtgtctt attaacatca atatacccct catctaagtg tatatcatta ggtgtaagtg
    12121 caaacagttc tcctgggcgc aagcctgtat taactgccac attatacaaa ttatcgtaaa
    12181 atgtcccttt acttgcttct aaaaactcta tctgttgttt tgctgttagt gtgaaagctt
    12241 taagttcttt atcagcccta agctttacac cttttgctgg atttttaatc attaagtcat
    12301 cttctattgc tctactgaac atatcattaa gtataacctt aatcttgttc tgccgttcat
    12361 atttataatt gtcatcagct attttatcaa ttagtgtttg aatatctgat ttaacaaagg
    12421 aatttatgtt gcgatttcct aaaaaaggtg atatattttt attgtatatg tgagtgtatt
    12481 ccctaagggt attagggcgt acacttttct ttttgtacac ttctatccaa cgattaaacc
    12541 aatcgtccag cttaatatca tctctaatgc ttgtaaattg aatattttct gctattgcaa
    12601 cagccaattc ttttttaact tctgataact ttgtgccata aatatattta atcttattaa
    12661 atctatcttt atatctccct tgatacacac cgtcctttcg ctgcgacaat cctacaccta
    12721 gttctttacc ttttaaatct tttcccattc aaaagctcct ttcttttgaa aaaagccttg
    12781 atatagacaa ccacatatta ctacatcaag gcatatattt caatatatct ctatatttcg
    12841 ttactttttt ctatatagtg ctcaaactcc ttacgcttaa caagcctctt attcccaact
    12901 cttaaaacaa atggacagct aatttcatta agcatactgc tgattctatt aattccgata
    12961 ttgctatatt cggacgcttc ttcaactgtt aatgtaactt tttcccatat aggaattgtt
    13021 ttaaccatgt catcagtcct ttctatcttg atttttatat ccttaactct tcttgaaatt
    13081 gttgctttgg ataacataag tctttgacta acctgttcta agctcatatt acccacaagc
    13141 aacttaaaaa tcctcaattc ctcatcagta aaattggcat tttcaattat ttcatcaagc
    13201 tccggcttag tcagtcccga aaacttcata agcctatctc cttatttaaa cttaatatgt
    13261 tctattcctg tttcttcgta taactgatta acaagctctt ccgctgtgaa taatccgtca
    13321 ttatagttat ctataagtgc tttaagttct ctctgtactt ttgttaatct ctgttgtccg
    13381 aaaccgaact tatcgtgtag cacccacata attaatatta atgctgattc aaaatttttc
    13441 ttctgctgtt cattgctaat tctattcatc tgaacacgta acatttgctc cttaaacttt
    13501 ttctgttctg ctttactcat attttcactt ctttcttaga aattgattgt cgtatcgcca
    13561 gtagtgcttg ctattgtcat tcttaagact tttacctctt tcgtagtctg tctgccagca
    13621 tttctgacat aattgtcctt gtggtctgtc aataggttct ccacaacgat agcacaagtg
    13681 attttccttg cgatattctt ttatattctg cctattttca gttctttttc tgtggatagc
    13741 attatcttta ctctggcata taaaacactt tgctttgccc tcaacagctt tagccttacc
    13801 acatctaaca catatgccag cttttctacg ttcagcgtat aagttttttg aatactgttt
    13861 aaatgcttca ttgttttttc ttcgcttatc atcacttaat gggtgattag ctctatattc
    13921 agctttgtta gccaaacatt ccggacatat cttttcatca cccacaagtt tatttttgcg
    13981 acattccggg caaattttaa actgcctgca aagttctcta gtttctctac tgtaagtcgt
    14041 ttgcttcttc ctacattctt cacaataaaa gccttttcta tcaagcggct tgccgcattt
    14101 agggcacaat ccattctctc ggcggtaatt atataatttc ttctgcggac taattggcgt
    14161 tgtttccact aaaaatcaac ctctcattct gtcaattcta tcttgtatct ctttgggtgc
    14221 ttcaatatac tcttctgcgt ttgtattttt actgataagg gcattttctt taatttgtaa
    14281 tgtatttata tctctttgga atttttgctc gatttgagcc ttatacgaat ttgcattcgc
    14341 ctttttgata agtgacttaa tgttatccgg catacgattt atttcattca cacgcttaac
    14401 aactgtttca taggttctca gaaaatttga ttgtattact gtttctatcg tctgataatc
    14461 tgatgtcgcc cagtttttaa ggttgtctgg cataccaacc gcctgtttta caagtggcgg
    14521 tagcttgtta aattcttcaa ccgccccata tgtgccgttc cttaatgctt tactgactaa
    14581 tccccaagct gccattccgt caagttcctg cggctgtgat attgtctgta tcttactcat
    14641 tatctgccct acatctggtg caaaaccgct agtattagtt gtaatgcaag cccttaacgc
    14701 ctgtaaaact aattcttctg gatattcagc aagcattata tgccaagcat taagagtaat
    14761 ctctttatct ggcggattgt agttaggata ataagcttgt atcgtcatta gaagttttcc
    14821 gacctgttcc cttgtcattt cattgcctcc atccattcat caaatacatt tttcttgcct
    14881 tgctgtttat tagaattatc ttcttttagc tcaaacagtc cttgccagca atggtctact
    14941 gactgattaa gaattttaat cgctaagtca ttatctccgc cagacagctt ttcgagagta
    15001 ttcatagccc tatgtaatgc cttgtcggtg catataggtt ttttaattct cttacgcatt
    15061 gtcacatact cgttaaatgc ttcatcaagt aattcatcat ctggataata acttttcttt
    15121 ttggatatta cgttagtaat atctttttct atattcttat cttctttaat ttcttctgtt
    15181 ctttcattct tactttcttt taatatagag tttgttaata gaatgttatc tgtttgttga
    15241 ttgtttgtta agttgcttgt tatttgtttg ttatcttgct tgttatccgt ttgatacaaa
    15301 ttgtagttaa ccacagtaaa tatcgtgaat ttgtttgttg ctttgcttgt tatttcgcct
    15361 gttaattgta agtgttttag cgaggtacga atttccatta cagacaaatt agtttctttt
    15421 gataattcag atattgaaga ggggaaagac cctctttcaa ttatcttgcc tttgtaattt
    15481 ccgtctttcc aataggcact tatcaacata tacataaaaa gtctgaatgt attaatgtca
    15541 ctccaccatt cccactttaa aatttttctg tcaattttaa taaaattgcc tgccataatt
    15601 acctcttcaa gttctgccac attgttactt cactaaatcg ttaatattaa ctctgaatcc
    15661 gtcaaattcc tttcctttac tcttgatata agctgttgta tcaaagaaca tcaagttacc
    15721 actattatca gctgccatac ttacgccatt tcttgtaaga ctgcctttga gtaggtcaag
    15781 taaaatctgt atttcctgct ttgtttcgtc tttcattatt tgcctctcca tatttcttca
    15841 tcaagaatat attgcctgat aaatctatct gcgtactgtg ggtgtatcat tgaccttgct
    15901 gtttttctgt ccactcccaa tggattatcg cttgtaacat atctttgctt cataacatca
    15961 actgcctcta atggttcaaa aataaaatta tttttaggtt gtaaaccaat gaaccaatat
    16021 tgtgtaggct ttttatagta atctccattc aaagtcctgt ctttgtctat gacattaggc
    16081 tttaggcacc aaaaatgtgt taaataatgc attccgctag tgcttaatgg attttctata
    16141 atcagcctta gatgttttct ttgacagaca ataacaaatt tgttaagtac ctcataaaac
    16201 aaacttaact gcctatgtct tttcattgac acctcacatt tttgctcaat agtgtaattc
    16261 ttatattgat aagctgtgca gcataaatgc ctagggcttt ggtctgaaaa ataagtgcaa
    16321 gggaaaaatg caagtatcaa atcatcaggg cttatcttat caaacaaact cggctcgcct
    16381 tgataccccc cctctatctc tttaaaaagg tcagtaacat agtcagtttc gttaaattca
    16441 ttctgaatat catagtcgta ggcttcaatt ccatacttct tgaaagcgtt tttgaatgtt
    16501 cctgactgtt caaataaaca atgtactatc attctaaatc caccaaaagg aaacctcggt
    16561 tttatgtgcg cacaacctat tcctttcttt gatttttagt tagttactgt ggttttctgc
    16621 ccgtctgaaa atattcgtca taagcgtcaa ccgtatagcg tatttcactc atagctatat
    16681 caactgttac atcttttcta tccaatgctc tttctgcata atctttaatt ctcatcatta
    16741 aagcctgtga tattactata tttgcatcgt tactcattct gaatcgccca ctttcaataa
    16801 atccataaac ttctcatact gcttctgcga taccttgttg tgcttcttat cgtctctaat
    16861 ttcgatttta aggtgttttt ctgcgataga ggataattcc ctcgctaaca cctttttgcc
    16921 ctgttgtaca ccctgcatat agcctttagg tgcttttctc tcgcctattg aaccactggc
    16981 acgattttct ccttgaccgc ctaaactgac atttctaagc tgataacctt tatcagcata
    17041 tagcttgatg taatacttct ccttttcgtc aagctggctt tcggggaaat tcagaaattc
    17101 aactcgccag ccataaggat ttttctcttt gtcgtacagc ttgtgtttgc gtaaactaag
    17161 gtctatgtgc tgttcatagc ctacaaggtg gcttgctaat ctgctaagtg tatgtactgc
    17221 ctgtccgata tacgcatact taaatccgtt ttcatcttct ctaagcaaga agtaaatccc
    17281 actcctgtca ttcagctttg gattaagctt caatagtcgc tttttatttt cctgttctat
    17341 tgccttgact cttgctatgt tctgataatt caagaattat cacctgcctt tacttcaaaa
    17401 ggattcacaa aattatcaat aggtttagct gccatactaa aagccgttgg ctgttcatta
    17461 atgatagttt caaatatttc agataaagcc ttatcgatat aatttctttt gtgaatatcc
    17521 tcaattagtt tatctgcgtc aatcagtctc atatattgtc tcctgccttt actatctcta
    17581 ttgccttttc aagaggaata agataattat tgctgttgcc gcttccatac aatcttacag
    17641 a
//
LOCUS       DBMDWL010000022        17620 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1086_length_17620_cov_3.467721, whole genome shotgun sequence.
ACCESSION   DBMDWL010000022 DBMDWL010000000
VERSION     DBMDWL010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 17620)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17620)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17620
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1086_length_17620_cov_3.467721"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="ACZPDN_02815"
     CDS             complement(<1..598)
                     /locus_tag="ACZPDN_02815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1297585.1"
                     /translation="MRIDLLCSGSKGNSCLIRHKDTQLLIDCGSTKKYLMESFQKVGA
                     CVDDTNGVLISHAHSDHVSQLKHFKHLDVYSYCDLKDALDKHDVLPNQKMIINDLSIQ
                     FIGLSHDSPHTIGFVVESETEKLVYVTDTGYVPNQIKPYIANADHYIFESNHDVNRLM
                     QTKRPMFLKQRILSDSGHLNNEDSSLNLTSLIKKKKKNI"
     gene            complement(588..1934)
                     /gene="dnaB"
                     /locus_tag="ACZPDN_02820"
     CDS             complement(588..1934)
                     /gene="dnaB"
                     /locus_tag="ACZPDN_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1297586.1"
                     /translation="MSREMPNAVSLEQSVLGSLMVYPRVIQDCQELDLHAEEFYLPSH
                     QQIYQAICTIHENGQPIDMNTVVTRLQETDQLESSQGTDYIIRLADSAISSATSRSYI
                     ETIKNKAQLRRLIYVADKISEEGYSSGEDIDTVLDDAERLVMDVTRHRRGSEFESSEE
                     IVTRVINELKELRTMKGVTGIETGYVLLDRMTNGFQRGDLIILAARPAMGKSALALNF
                     AAQVAKRNEGAVAIFSLEMPSDSMMKRLMSSESQVYSDKLRSGKLTNEEMSRLYEAGS
                     HLSERKIYIDDTSSIKVSQIFSKCRKLKSENGSISLVVIDYLQLITGTRADSRQQEVS
                     DISRNLKILAKEMECPVIALSQLSRKVEERTDHEPQLSDLRESGSIEQDADIVMFLYR
                     SNYYDKDKESQEETEVVDLSLAKHRNGGIGKIQLAFEKNISRFTNLEASGDDIYAH"
     gene            complement(1936..2385)
                     /gene="rplI"
                     /locus_tag="ACZPDN_02825"
     CDS             complement(1936..2385)
                     /gene="rplI"
                     /locus_tag="ACZPDN_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1297587.1"
                     /translation="MKVILLKDIRKLGKKDDVVEVSDGYARNFLFKNKSAIAYTKGSA
                     KVLDKQMEERAEHEAELKKEAEALSETLKGITLEFRLSTGKNGATFGSVSSKQIVEAL
                     AKKGVRVDKRKLQLDQSIASLGVTRVPVDLYHGQVIGEIVVHVSEKA"
     gene            complement(2366..4336)
                     /locus_tag="ACZPDN_02830"
     CDS             complement(2366..4336)
                     /locus_tag="ACZPDN_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1297588.1"
                     /translation="MKQFEKWRSLCVTGLVLGGILSLLLFLKKEYIFLVAALVIGIIL
                     MCAFFGFWKSLKKHGMKTEVDISRVLGKDAKDALNFGDVGIVTYNEEYIVTWASPFFK
                     EKGINLTNNKLTSWISNIRTLFDDDVDMVIGKSEGRIYEITRKRDAQILYVKDITDYY
                     NMRQQYLDNEIVIGLLQLDNYMEYQSYENEEIMAQINTHLRASLVTWAKENKMFVRRL
                     RSDRFLVILNKEILAQVRKQNFTILQLIKDEANKLDVSITLSMAFAYGSNDFTVLDDM
                     VNELIELAQSRGGDQAAIRASGGIVQFVGGNSETSSTRSKVRVRIMAQSIQEAIMESN
                     RVYIAGHVMSDFDCMGACLALSSWVHALGKEVYIVLKDVPRDEQLQEVMNSFISVIQE
                     RHTLITPDEAMASMDFNSDLLIMADHGIPAISSVKEFVNQCNRILVIDHHRRSDAFVK
                     NTLLTYVESSASSACELIVELLQNIPNHIPIYEMEATIMYLGILVDTNRFKMHTDART
                     FEAAAALQNWGANTKRAEKALCEDYLYFSMKNALIQQAKPYYNHFMIAAIEDETLEKT
                     MMAQVSQALLLIKGCIASFTIAKVKNNSNAVAVSARSDGSYNVQKIMEKLNGGGHFSA
                     AAVERADVSVQQMKDMILKCIEEEQNNESNIA"
     gene            complement(4348..5202)
                     /locus_tag="ACZPDN_02835"
     CDS             complement(4348..5202)
                     /locus_tag="ACZPDN_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HIR1297589.1"
                     /translation="MIKTKQITKGAMVCAIYGALLFLNQQSALMIESSASWIFAFPVL
                     IYTAMSGVKMGVLVTVCMGLMTMFFGGFTTWFYSWTSLLIGFLYGVGVSKHVKNMTNF
                     IFCFILSVLANLCIILLWSTLFGIDFYEDFRLISHYIPFISLKVFTYLMIGMLGLLQA
                     LCIHLVALMECMRMHMDIVPLQPLSKVQSPRWVGVCSIVVWIAFFFIQNVLKCPTGIQ
                     DLAQILFFVDCALLLYFGVVYFMSLCVKYQKKKWSFLSIFGAFIPGLNLLWMFMGELD
                     CLLQLRKE"
     gene            complement(5252..5482)
                     /gene="rpsR"
                     /locus_tag="ACZPDN_02840"
     CDS             complement(5252..5482)
                     /gene="rpsR"
                     /locus_tag="ACZPDN_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1297590.1"
                     /translation="MAFKKQRMGRKKVCYFTKNKIKEIDYKDVELLKRFINANGKIIP
                     RRVTGTRAKYQRMLATAIKRARQMALLPYVVD"
     gene            complement(5500..6018)
                     /gene="ssb"
                     /locus_tag="ACZPDN_02845"
     CDS             complement(5500..6018)
                     /gene="ssb"
                     /locus_tag="ACZPDN_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1297591.1"
                     /translation="MINRVVLVGRLTRDPELRKTQSGTSVCSFTMAVGRRVSTQGQPD
                     ADFINCVAWNKTADLMTQYLHKGSLIGLEGRIQTRSYDNQQGQRVYVTEVVAESVQFL
                     EPKNAQSNYSAGSQDYGMNQGFAQNTYTAPAQNGYNQNSYSNPVQNTNNAFDSTFNAN
                     DSLDIASDDLPF"
     gene            complement(6035..6328)
                     /gene="rpsF"
                     /locus_tag="ACZPDN_02850"
     CDS             complement(6035..6328)
                     /gene="rpsF"
                     /locus_tag="ACZPDN_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR1297592.1"
                     /translation="MKKYEIMYIVNASLDDAAFKTVSDRLHATITENGGSIDKVDDWG
                     VKEFAYEIDHMKKGHYVVINVTANNAGVFEFQRLSRISNNVIRIMVVKTESEK"
     gene            complement(6436..7659)
                     /locus_tag="ACZPDN_02855"
     CDS             complement(6436..7659)
                     /locus_tag="ACZPDN_02855"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1297593.1"
                     /translation="MMEFVYAYAYFVLYAFLGWVCEDLYCGIPAKKFINRGFFYGPYC
                     PIYGVGALLVLYPLLFVKDYPILVFILGVIITSTLEYITSWVMEILFKTRWWDYSERF
                     MNINGRVCLLNSTLFGIMSIVVVYIIHPVIQDVVLDIPLTALMSFLSAFTIGFGIDCV
                     FTVLALLRRKKVFQKVQTQMEAFKKDFEAESQLRVQEAQEAFQEWMLSRPDLSQRIEE
                     FQSSLDNFSLEAKKHISKAFPERRISTEIRNIVADGKLALERRRLNANKQVEYCELTN
                     MVMVQDEKGNVLVQNSKNPNGYTLPTGHIELGESFVQSAIRDVKKKTGLDVDQLELCG
                     TKQFITSDNCRHIVFLYKTNVFSGKINEESYWIPLSNIQDYKLVSGLSDVLEVFAGTK
                     FNELYYNAQYQLKKY"
     gene            complement(7656..8912)
                     /locus_tag="ACZPDN_02860"
     CDS             complement(7656..8912)
                     /locus_tag="ACZPDN_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR1297594.1"
                     /translation="MSLEYTLFNSDMKKDYTILVPSMLPIHFRLLTPVFAKSGYKLEV
                     LTNEGEQVREEGLAHVHNDTCYPALLVIGQMIDALKSGKYDMNHVACAITQTAGGCRA
                     SNYLSLLRKALAMEGWQHIPCLSANANGLEKGSSVEFTMPLILRILYGALYGDALMWL
                     GNQVKPYELEKGATDKAVNQFIDELIPMFEHGSYKQSKRIYKKMLEMFKSIPQDRSQT
                     KIRIGIVGEIYMKYSPLGNQHLEDYLIEEGFEPVLSGVMDFALYCVENSIIDYEYYHM
                     HEKNHYIYNIVKDVIMRMQKTFRDMVKKEGTFIAPDDFSEVIDNGKAFIDPGVKMGEG
                     WLLTGEVVSLIKSGVTNVISAQPFGCLPNHIVAKGMVRKIKDEYPKANIVAIDYDPSA
                     SKVNQENRIRLMLANAKLSEEMKASV"
     gene            complement(8915..11833)
                     /locus_tag="ACZPDN_02865"
     CDS             complement(8915..11833)
                     /locus_tag="ACZPDN_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR1297595.1"
                     /translation="MGYRIGFDIGSTTIKAVVLDENNQICYKSYERHKAQVRQKALDK
                     LVELKKYTKEPFKFAISGSGALGLCEQGELPFVQEVFASATGVSKLYPETDCAIELGG
                     EDAKILFFQGSVEQRMNSTCAGGTGAFIDQMSTLLNMSLEEMNEASLNYHRLYPIASR
                     CGVFAKTDIQPLINQGVDKCDLCASIFQAVVDQTITSLAQGRKIEGNILFLGGPLYFM
                     SGLRKRFVETLKLSDVQVNCPETAINFVALGTAICADQEYTYDELYKALEDLVHAPAK
                     LTESKPLFESEEDYQKFIGRHKSHDAKYAKLKDYAGKAYLGIDSGSTTTKLVLLDEND
                     AILYDSYTSNKGNPLDVVLEDLKKIYETNPNIKIYGAYATGYGEELMRHAFRLDGGIV
                     ETMAHYTAARSFNPEVDYILDIGGQDIKCFKIRDGHIDDIVLNEACSSGCGSFIETFA
                     KSLGYDAKEFATLGLKSKHPVDLGTRCTVFMNSGVKQAQKNGASIEDISAGLCKSVVK
                     NALYKVIRAKNKDDIGKHIVVQGGTFQNDSVLRCFEQELGLEVIRPSIAPLMGAYGAA
                     LYAKKLHRTRSSILNYEQLVKFTHKSQSVACQGCNNHCSLTINTFNDGTRLVAGNKCE
                     KMVHNLDAKKEELPDLYKIKTDMLDAQKKKFKHDKKIGMPLVLNNYDLLPFWTRFFDS
                     LGYEIVWSTPSTKEMYHSGQQSIPSDTACFPAKVVHGHIQQLIDKDVDVIFYPCMTYN
                     VDEHQSDNHYNCPLVAYYPEVIAANMDFGKTKIVYPFISFDDNATFAKAIRMHFEKVG
                     IHFSEKDIIIAKNAATNEYEAFHEQLVKEHRKAIAYARENNHPIIVLCGRPYHLDPLI
                     NHQMNQLLTQLGFVVVSEESVPRQIGHKVNVLNQWTYHARLYQAAHYVVDNKDMNLIQ
                     LVSFGCGIDAITSDEVRDILKDGNKFYTQIKIDEIDNLGAAKIRLRSLKEAIAQKKGV
                     "
     gene            12046..13281
                     /locus_tag="ACZPDN_02870"
     CDS             12046..13281
                     /locus_tag="ACZPDN_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1297596.1"
                     /translation="MKNKLTKLEFKWICYDMGNSAFILLVATILPIYFNYLSSSQGVA
                     EYNYLSYWSYAASISTLIVALAGPILGTLADYKDHKKKIFLTCAMLGALALACFWIPS
                     SWFAFLVLFIAAKVAYSLSLIVYDSMLTDITTEDRMDAVSSKGYAWGYIGSVVPFIIS
                     LVFVLMYDKMGMTLKAAMMIAFVLNAVWWIAFTLPLVKSYKQKFYIEPEAHPVLDTFA
                     RLKDTLIKAKEQKKVFLFLFAFFFYIDGVYTIIDMATAYGTSLGLNTTGLLLALLLTQ
                     IVAFPASLTFAVLSKTKDTASLIKVAIIAYFLIALFAVQLDKQWEFWVLAVAVGCFQG
                     GIQALSRSYFAKIIPENASGEYFGLFDICGKGASFLGTMLIGVVTQITGKQNLGVAAL
                     SIMFVIGYLIFNKVSKMDD"
     gene            complement(13314..14270)
                     /locus_tag="ACZPDN_02875"
     CDS             complement(13314..14270)
                     /locus_tag="ACZPDN_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297597.1"
                     /translation="MILADKIIEERKKNGWTQEDLAQKLGVSRQSVSKWESAGAIPDL
                     KKIIQLADLFGVSTDYLLKDEVEKENTETICDTDCELHRVTMEEANAYMNEKKKAAPM
                     LANAATLCILSPISLFLVSTFNDSLAIGIACAILLGMVALAVFIFIKTGIKLSPLQYL
                     EQEPFETEYGVTGLVKEKNQSYQEHYSFSLACGVVLCILSVIPLIIAGCMEASDYICT
                     LCLAVLLILVSVGVNILIRVSIIKSSYDTLLQEGDYTKKEKLVKKKTETFTGVYWCLV
                     TAVYLGWSLWTKQWDVTYMIWPVAAVLYAAMYGIVKMIMKVD"
     gene            complement(14394..17468)
                     /locus_tag="ACZPDN_02880"
     CDS             complement(14394..17468)
                     /locus_tag="ACZPDN_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297598.1"
                     /translation="MQNKKKNRFSKIMRILMTALTSLLIVLILIIILMVSRIQGTARV
                     VNYAGLVRGKTQRIVKLEDARMPQDDMIADVKGYIKGLRFGSEELDLVSLDDKAFQAK
                     MEELDAYFDTLKQEIDLVRQVGYENTSIIEKSEIFFNLCDVATGLAESYSQRIATRLK
                     QFETLTVIDIVILVFMILYELLKALRYAKANRELKSKIYLDEATGLPNKNKCEEILTL
                     EAEQNMAICVFDLNNLRIINNQQGHERGDLYIRSFAKSLRKGVDENQFVGRCGGDEFI
                     AFYKNVTKEDVKRNLENIKKECAKCSEIPLSYAAGFAYSNDFSKLTMRELFCQADKNM
                     YIDKNQAKIKEAKQKRNLILNVIQQLKEKGFNFSDCIYCDAKTDAYFTLRASHSFFLA
                     EDGNYSGAIEQILNELFNEDKRMDYRNVLNVNSLNKYVTKDNPIFEIPYYHQMNNAEL
                     KGKIMVVYLDSDEYGNLHHFVLGFKMYSDTIEMDEKKQLMRYYDQLKQSILENANYIE
                     ALMNMAQSIFSVNLTQNQIDGIYDKYMVENIRPALPCSYEAYFKQWKSNVLEDCLGSF
                     GIVESCQNLLDRYKAGDKYVTIEYQIKMPDDRVIWVQEMILMSEEIIYDIDIQSERNI
                     VRAILLFRNTSAFHEKEDREKEKLQLAYQKVDLESKAKTDFMNRMSHDIRTPINGILG
                     MMQIIRKNWGDMDKLDDSLNKMEVLTKHLNELVEDILNMSKLESDHMEIVNEPFDLNY
                     MVEELNSLIDAQVSLQNITYHKHMENVVHTHLIGDALQLRRILINLLTNAIKYNKPNG
                     TIDMYVREIKSDDSKVTFEFEIKDTGIGMSKDYIEDHLFTPFSQAKQDARTRYEGTGL
                     GMSIVKGLVDKLGGNIEVKSEVGVGTQIKVVLTYQLDTSKNGKQDSSKLDLKDKRILL
                     VEDNEINMEVAEFYLNAVHANVDKAWNGLEAVEKVKEQPNQYSLILMDIMMPKMNGDE
                     AAKCIRKINPSIPIVAMSAQSEYSIDQTIMNDSISKPIDEQKLNHILSKYVA"
BASE COUNT         5637 a         3600 c         2402 g         5981 t
ORIGIN      
        1 caatattttt tttttttttt ttaatcaatg aagttaaatt taaacttgaa tcttcattat
       61 tcaaatgtcc actatcactt aaaattcgct gcttcaaaaa cataggacgc tttgtctgca
      121 tcaaacgatt gacatcatga ttactttcaa aaatataatg atcggcattg gcaatatatg
      181 gcttgatttg attaggcaca taccctgtat ccgttacata tactagcttt tccgtttctg
      241 attcaaccac aaaaccaatg gtgtgtggtg aatcatgtga taaaccaata aattgaatac
      301 ttaaatcgtt aataatcatt ttttgatttg gtaatacatc atgcttatct aaagcatcct
      361 ttaaatcaca ataggaatat acatccaaat gtttaaaatg ttttaattgc gaaacatgat
      421 ctgaatgcgc atgactaatt aaaacgccat ttgtatcatc cacacaagcc ccaacctttt
      481 gaaagctttc cattaaatac tttttggttg aaccacaatc aatcaacaat tgcgtatctt
      541 tatgtcgaat caaacagcta ttgccttttg aaccactaca caacaaatca atgcgcataa
      601 atatcatctc cggatgcttc taaatttgta aagcgagata tgtttttttc aaaagccaat
      661 tgaatctttc cgataccacc attacgatgt tttgccaaag acaaatctac cacttccgtt
      721 tcttcttgtg attccttatc tttatcataa taattactac gatataaaaa catgacaata
      781 tcggcgtcct gctcaatcga tccagactca cgtaaatcgg acaactgcgg ttcatgatcc
      841 gttctttctt caaccttacg agaaagctga gaaagcgcaa taacgggaca ttccatttct
      901 tttgccaaaa tctttagatt acgcgaaata tcactaacct cttgttgacg agaatcggca
      961 cgtgttccgg taatcagctg caaatagtca attacaacta aagaaataga gccgttttca
     1021 ctctttaatt tacgacactt tgaaaatatc tgagatacct tgatactact tgtatcatca
     1081 atgtaaattt ttctttcact caaatgcgaa cctgcctcat acaaacgaga catttcttca
     1141 tttgtcaatt taccactacg aagcttatcc gaatatacct gagattcaga actcattaaa
     1201 cgtttcatca tcgaatcact cggcatttcc agtgagaata tcgcaacagc tccttcattt
     1261 cgttttgcaa cctgagctgc aaaattcaag gccaaggcac ttttccccat ggccggacga
     1321 gctgcaagaa taattaaatc acctcgttga aagccatttg tcatacgatc caacaagaca
     1381 taacctgttt cgattccagt tacacctttc atcgtacgaa gctcttttaa ttcattaatc
     1441 acacgagtaa cgatttcttc actcgattca aattcgcttc ccctacgatg tcgagttaca
     1501 tccataacta aacgttccgc atcatctaaa actgtgtcaa tatcttcacc agatgagtat
     1561 ccttcttcac tgattttatc cgcaacataa atcaatcttc ttaattgtgc cttattcttg
     1621 attgtttcaa tataagatct tgatgttgca cttgaaatag ctgaatccgc caaacgaata
     1681 atataatccg ttccttgact agactctaac tgatctgttt cctgtaaacg cgtaaccact
     1741 gtattcatgt caataggctg accattttca tgaattgtac agatagcctg ataaatctgc
     1801 tgatgagaag gcaaataaaa ttcttctgca tgtaaatcaa gttcctgaca atcttggatg
     1861 actcttggat ataccattaa agaaccaaga acagattgtt ctaaggaaac cgcattaggc
     1921 atttcacgag acatattatg ccttttcact tacatggaca acaatttcac caatcacttg
     1981 cccatgatat aagtcaactg gaacacgagt tacacccaaa gaagcaattg attgatccaa
     2041 ttgaagtttt cttttatcaa cacgaactcc ttttttagcc aatgcctcaa caatttgttt
     2101 acttgaaacg gaaccaaaag tagctccatt ttttcctgta ctcaatctaa actctaaagt
     2161 aatacccttt aaagtttcac ttaaagcctc agcttctttt ttcaattctg cttcatgctc
     2221 tgctctttct tccatttgtt tatctaaaac tttggcagag ccttttgtat aggcaatggc
     2281 agacttattt ttgaataaga agtttcttgc atatccatca cttacttcta ctacatcgtc
     2341 tttctttcct aatttacgaa tatctttaag caatattact ttcattattt tgttcctcct
     2401 ctatacattt taagatcata tccttcatct gttgtacgga tacatctgcc ctttcaacag
     2461 cagccgcact aaagtgacca ccaccattta atttttccat aatcttttga acattgtatg
     2521 aaccatcact acgagcactt accgcaaccg catttgaatt gttcttaact ttcgcaattg
     2581 taaaacttgc gatacacccc ttgatcaaaa gcaacgcttg tgatacttga gccatcatcg
     2641 ttttttctaa tgtttcatct tcaatagccg caatcataaa atgattataa taaggcttag
     2701 cttgctgaat caatgcattc ttcattgaga aatacaaata atcttcgcac aatgcttttt
     2761 cggcacgctt tgtgttagca ccccaatttt gtaaagcagc tgcagcttca aatgtacgcg
     2821 catccgtatg cattttaaaa cgatttgtat ccactaaaat acccaaatac ataatcgttg
     2881 cttccatttc ataaatagga atatgatttg gaatattttg aagcaattca acaatcaact
     2941 cacaagctga acttgcactg ctttcaacat atgtcaacaa agtattctta acaaacgcat
     3001 cactacgtct atgatgatca atcacaagaa tgcgattgca ctgattcaca aattctttaa
     3061 cacttgaaat agctggaata ccatgatctg ccataatcaa tagatctgaa ttgaaatcca
     3121 tagaagccat tgcctcatcc ggcgtaatca aagtatgtct ttcttgaata acagaaataa
     3181 atgaattcat tacttcttga agttgctcat ctctaggtac atcttttaaa acaatatata
     3241 cttctttacc taacgcatgc acccagcttg ataaggcaag acaggcaccc atacagtcaa
     3301 aatcactcat cacatgtcct gcaatataaa cacgattgga ttccatgata gcttcttgaa
     3361 ttgattgtgc cataatacga acacgcactt tagaacgagt cgaacttgtc tctgagtttc
     3421 cacccacaaa ctgcacgatt cccccactag cacgaatggc cgcctgatct ccaccacgac
     3481 tttgagcaag ttcaatcaat tcattgacca tatcatctaa aaccgtgaaa tcattggatc
     3541 catacgcaaa agccatactt aaagtaatcg aaacatccaa tttattcgct tcatctttga
     3601 tcaactgtag aattgtaaaa ttctgcttgc gtacttgggc caatatctct ttatttaaaa
     3661 taactaagaa acgatctgaa cgcaaacgtc tgacaaacat tttattttct tttgcccaag
     3721 taaccaaaga tgctcttaaa tgcgtattga tttgcgccat gatttcttca ttttcataag
     3781 attgatattc catataatta tcgagttgca acaaaccaat cacaatttca ttatctagat
     3841 actgctggcg catattataa taatctgtaa tatcttttac atacaaaatt tgtgcatctc
     3901 ttttacgcgt gatttcatag atacgacctt cacttttacc aatgaccata tctacatcat
     3961 catcaaataa agtacgaata ttactgatcc aactcgttaa tttattattt gttagattga
     4021 ttcctttttc tttaaagaaa ggagaagccc acgttacaat atattcttca ttgtatgtta
     4081 caatacccac atctccaaaa tttagtgcat ccttggcatc tttacctaac actcttgata
     4141 tatcgacttc tgttttcata ccatgcttct ttaacgactt ccaaaagcca aaaaaagcac
     4201 acattaaaat aatccctata actaaggctg caaccaagaa tatgtattct tttttcaaaa
     4261 ataacagaag cgatagtatg ccacctaaaa caaggcctgt aacgcatagg ctacgccact
     4321 tttcaaattg tttcatatag aaaacctcta ttcttttctc aactgtaaaa gacagtctaa
     4381 ttctcccata aacatccaca ataaattaag tccaggtata aaggcaccaa aaatagatag
     4441 aaatgaccat ttcttttttt gatactttac acataaactc ataaaataaa caacaccaaa
     4501 atataaaagt aaagcacaat ccacaaagaa taaaatttgt gctaaatctt gaatccccgt
     4561 tggacatttt aacacatttt gaataaaaaa gaacgcaatc cataccacaa tagagcatac
     4621 tccaacccat ctaggacttt gtactttaga taatggttgt aacggaacaa tatccatatg
     4681 catacgcatg cattccatca atgcgactaa atgaatacat aaagcttgca aaagtcctaa
     4741 cattcctatc atcaaatatg taaatacttt taaactgata aatggaatgt aatgtgaaat
     4801 caagcggaaa tcttcataga aatcaatacc gaacaatgta ctccataata gaataataca
     4861 taaattagcc aaaacagata gaataaaaca gaaaataaaa tttgtcatat tcttaacatg
     4921 tttagacaca cccacaccat ataaaaagcc aatcagtaat gatgtccaac tataaaacca
     4981 agttgtgaaa ccaccaaaaa acatggtcat caaacccata caaacggtta ccaaaactcc
     5041 cattttaacg ccgctcatgg ctgtataaat gagtacaggg aaagcaaaaa tccaggaagc
     5101 actggattca atcattaatg cactttgttg atttaaaaat aataaagctc cataaatagc
     5161 gcaaaccata gcgccttttg tgatttgttt tgtttttatc atacgactat tatataaaaa
     5221 taaaaaaaag acttcaatat atgaagtcta attagtcaac cacgtaaggt aacaaagcca
     5281 tttgacgagc acgtttaata gctgttgcta acattctttg gtattttgca cgagttccag
     5341 tcacacgtct aggaataatt tttccgttag cgttgatgaa acgttttaat aattctacgt
     5401 ctttgtagtc gatttcctta attttgttct tagtgaagta acaaactttt ttacgaccca
     5461 tacgttgttt tttaaatgcc ataagtaagt ccttcctttc tagaatggta aatcatcact
     5521 agcaatatct agtgaatcat tcgcattgaa tgttgaatca aatgcgttgt ttgtattctg
     5581 cacaggattt gagtatgaat tttgattata tccattctgc gcaggagcag tatatgtatt
     5641 ttgagcaaaa ccttgattca ttccataatc ttggcttcca gctgagtaat ttgattgtgc
     5701 attttttggt tctaagaatt gaacactttc tgccacaacc tctgtaacat ataccctttg
     5761 tccttgttga ttatcataag aacgagtttg gatacgtccc tctaaaccaa tcaatgaacc
     5821 tttgtgtaaa tactgtgtca tcaaatcagc tgtcttattc caagctacac agttaataaa
     5881 atctgcatct ggctgtcctt gcgttgaaac acgacggcca actgccattg tgaatgagca
     5941 aacacttgtt ccactttgcg ttttacgtaa ttcaggatca cgtgttaaac gcccaactaa
     6001 tactactcga ttaatcatat ttatttaacc tcgattattt ttcgctttca gtttttacaa
     6061 ccataatacg aataacattg ttactgatac gagacaaacg ttggaattcg aaaactcccg
     6121 cattgtttgc agttacgttg ataactacat agtgtccctt cttcatgtga tcgatttcgt
     6181 aagcgaattc tttcacaccc cagtcatcta ctttgtcaat tgaaccaccg ttttcagtga
     6241 ttgttgcatg taaacgatct gaaactgttt tgaacgctgc atcatctaaa gatgcattca
     6301 caatgtacat aatttcatat tttttcatgt gtacacctcc ctttggtctt ttttggcttc
     6361 tttcgaagca gagaacaggc ctaaacctgc tttgttatta taacaaaaaa agactttgct
     6421 tgtaaaccac aaagtctaat atttttttaa ttgatattgc gcattatagt aaagctcatt
     6481 aaatttagtt cccgcaaaca cttcaagcac atcgcttaag cctgaaacca acttataatc
     6541 ctgaatattc gataatggaa tccaatagga ttcttcattt atctttccac taaacacatt
     6601 cgttttatat aagaatacaa tatgtctaca attatcactt gtgataaatt gtttggttcc
     6661 acaaagctct aattgatcaa catccaaacc tgttttcttt tttacatcac gaattgccga
     6721 ttgtacaaag gattcaccta gttcaatatg acctgttggt aatgtgtatc catttggatt
     6781 tttgctattt tgaactaaca catttccttt ttcatcttga accatgacca tatttgttaa
     6841 ttcgcaatat tcaacttgct tattcgcatt caatcgtctt ctttcaagtg ctaatttacc
     6901 atctgctaca atattacgaa tctctgtaga aatacgacgt tctgggaaag cttttgaaat
     6961 atgttttttt gcttctaaag agaagttatc taaactgctc tggaattctt caatacgctg
     7021 ggataaatca ggacgagaca acatccattc ttggaaagct tcttgcgctt cttgaacacg
     7081 caattgactt tcggcttcga aatccttttt gaaagcttcc atttgtgttt gcaccttttg
     7141 gaatactttc ttgcgacgaa gtaaggcaag cacagtaaat acacagtcaa taccaaaacc
     7201 aatcgtaaag gctgacaaga aactcatcaa tgccgtaagt ggaatatcta acacgacatc
     7261 ttgaatcact ggatgaatga tataaaccac cacaatagac ataatgccaa ataaagtgga
     7321 gttcaacaaa catacgcgcc cattaatatt cataaatctt tcagagtaat cccaccatct
     7381 tgttttaaat aaaatttcca ttacccaact cgtaatatat tccaaagtac ttgtgataat
     7441 gacacccaaa ataaatacaa gtattggata atctttcaca aataacaaag gatataaaac
     7501 cagtaaagca cctactccat agattggaca atatgggcca taaaagaagc ctcggttaat
     7561 aaacttcttt gcaggaatcc cacaatataa gtcttcacac acccatccta aaaaggcata
     7621 caaaacaaag taggcatagg catatacaaa ttccatcata cactagcctt catttcttca
     7681 cttaatttag cgttggccaa catcaatcgt atacgatttt cctgatttac tttagatgca
     7741 cttggatcat aatcaatcgc aacaatattc gcttttggat actcatcttt gatcttacga
     7801 accatacctt tggcaacaat atgatttggc agacaaccaa atggctgtgc actaatcaca
     7861 ttagtaacac cagatttaat aagtgaaaca acttcacccg ttaaaagcca accttcaccc
     7921 atttttacac ctggatcaat aaatgctttt ccgttatcaa tcacttcact aaaatcatct
     7981 ggtgcaataa aagtaccctc ttttttcacc atatctctaa atgttttctg catgcgcata
     8041 attacatctt ttactatatt atatatatag tgattttttt cgtgcatatg ataatattca
     8101 taatcaataa ttgaattttc tacacaatat aacgcaaaat ccatcacacc ggatagaacg
     8161 ggttcaaaac cttcttcaat taaatagtct tctaaatgtt gatttcctaa tggagaatat
     8221 ttcatataga tttctccaac aattccaata cgaatctttg tctgactacg atcttgtgga
     8281 atggacttaa acatttctag cattttttta taaatacgct tagattgttt ataagaacca
     8341 tgttcaaaca ttggaatcaa ttcatcaata aattgattga cagccttatc tgtagctcct
     8401 ttttcaagtt cataaggctt cacctgattg cctagccaca ttaaggcatc tccatataaa
     8461 gctccataaa gaatgcgaag aatcaaaggc attgtaaatt caacactgct acctttttct
     8521 agaccattcg cattggcaga taaacaagga atgtgttgcc atccttccat agccaaagct
     8581 tttctcaata aagaaagata attgctagca cgacatcctc ctgctgtttg tgtaatggca
     8641 caagccacat gattcatatc atacttacca ctttttaatg cgtcaatcat ttgaccaatt
     8701 actaataatg ctggataaca agtatcatta tgcacatgag ctaagccttc ttcacgtact
     8761 tgctcccctt catttgtcaa aacttctaat ttatacccac ttttcgcaaa gacaggtgtc
     8821 aataagcgaa agtgaatagg aagcatagaa ggaactaaaa ttgtataatc cttcttcata
     8881 tccgaattaa ataatgtata ttctaaactc aattttagac tcctttcttt tgggcaatcg
     8941 cctcttttaa agaacgcaaa cgaattttag cagcacccaa attatcaatt tcatcaattt
     9001 tgatttgtgt ataaaattta tttccatcct ttaatatatc tcgaacctca tcacttgtaa
     9061 tggcatcaat accacatcca aagctaacaa gttgaattaa attcatatct ttattgtcaa
     9121 ccacatagtg tgctgcttga tataatcttg catgataagt ccattgattt aagacattca
     9181 ctttatgtcc aatttggcga ggaacactct cttcactgac aaccacaaaa ccaagttgtg
     9241 tcaacaattg attcatctgg tgattgatga gaggatctaa gtgatatgga cgtccacaaa
     9301 gcacaataat tggatgattg ttttcacgtg catacgcaat ggcttttcga tgttccttta
     9361 ctaattgttc atgaaaagct tcatattcat ttgtagctgc atttttcgca ataataatat
     9421 ccttttcact aaaatgaata cccacctttt caaagtgcat gcgtatagct ttcgcaaaag
     9481 ttgcattgtc atcaaaacta ataaatggat atacaatttt tgtcttacca aaatccatgt
     9541 tggccgcaat cacttctgga tagtaggcaa ctagtggaca attataatga ttatccgatt
     9601 gatgttcatc tacattatat gtcatacatg gatagaatat tacatctaca tctttatcaa
     9661 tcaattgttg aatatgtcca tgcacaacct tggctggaaa acatgccgta tcacttggaa
     9721 tactttgttg tccagaatga tacatttctt tagtactcgg tgttgaccac acaatttcat
     9781 agcctaaact atcaaaaaaa cgcgtccaga aaggtaataa gtcataatta tttaagacaa
     9841 gtggcatacc aatcttttta tcatgtttga atttcttttt ttgtgcatcc aacatatctg
     9901 ttttgatctt atataaatct ggaagttctt ccttctttgc atctaaatta tgcaccatct
     9961 tttcacattt gtttcctgca actaaacgcg ttccatcatt gaatgtatta attgtcaatg
    10021 aacaatggtt gttgcaacct tggcaagcaa cagattgtga tttatgcgta aatttaacaa
    10081 gttgttcata attcaaaata ctacttcgag tacggtgtaa ttttttcgca tataaagccg
    10141 ctccgtaagc acccattaaa ggtgcaatgc ttggtcgaat gacttcaaga cctagctctt
    10201 gttcaaaaca acgaagtaca gaatcatttt ggaaggttcc accttgtaca acgatatgtt
    10261 ttccaatatc atctttattt tttgcacgaa ttactttata tagtgcattc tttacaacag
    10321 atttgcaaag tccagctgaa atatcttcaa tactagcccc attcttttgg gcttgtttaa
    10381 cccctgaatt cataaataca gtacaacgtg ttcctaaatc gactgggtgt tttgacttta
    10441 atccaagtgt cgcaaattct ttggcatcat aacccaatga ttttgcgaac gtttcaataa
    10501 atgaaccaca tccactcgaa catgcttcat tcagtacaat atcatcaatg tgtccatcac
    10561 gaattttaaa acacttaata tcctgcccac caatatctag aatgtagtct acttctggat
    10621 tgaaagatcg agcagctgta tagtgtgcca ttgtttcgac aattccacca tctaaacgga
    10681 aggcatgacg catcaattct tcaccatatc ctgtggcata ggctccataa atcttaatat
    10741 ttggatttgt ttcataaatc ttctttaaat cctctaacac aacatctaat ggatttccct
    10801 tattcgatgt ataagaatca tatagaatcg catcgttttc atcgagtaaa acgagttttg
    10861 tggtcgtcga accggaatcg attcctaaat atgctttacc agcataatcc tttaactttg
    10921 catattttgc atcatgtgat ttatgacgtc caataaattt ttgatagtct tcttcacttt
    10981 caaacaaagg ttttgattct gttaatttag ctggtgcatg cactaaatct tctaaagcct
    11041 tatataattc atcatatgta tattcttgat ctgcacaaat tgcagttcct aaagccacaa
    11101 agtttatggc tgtttcagga caattcactt gtacatcact taatttcaaa gtctcaacaa
    11161 atcgttttct aagtccactc ataaaataaa gtggtcctcc taaaaacaat atatttcctt
    11221 caatctttct tccttgtgct agcgaagtaa tggtttgatc tacaacagct tgaaaaatac
    11281 tcgcacacaa atcgcattta tccacacctt gattgatcaa tggctgaata tctgttttcg
    11341 caaacacacc acagcgagat gcgatggggt ataaacgatg atagtttaaa ctcgcttcat
    11401 tcatttcttc caaagacata tttaataagg tagacatttg gtcaataaat gcgcctgttc
    11461 caccagcaca agttgaattc attctttgtt ctacacttcc ttgaaagaaa agaattttcg
    11521 catcttctcc accaagttca attgcacaat cggtttctgg atataattta ctgactccag
    11581 tcgcacttgc gaatacttct tgaacaaaag gcaactcacc ttgttcacat aatcctaaag
    11641 cacccgatcc actgatcgca aatttaaacg gttcttttgt atatttcttt aattctacta
    11701 atttatctaa agctttttga cgcacttgcg ccttgtgtct ttcataactt ttataacaga
    11761 tttggttatt ttcatctaaa acaaccgctt tgattgttgt acttccaatg tcgaatccta
    11821 tacgataacc cataatctcc tccattttct ttttttatat cagtattatt atactcattt
    11881 ttcatttttt ttcatgtttt cagttcaaaa ttaagacaac gcaataaata tgttcacttt
    11941 gttgtagatt attcgcgaca aaacatatat cattaaatta acaaatagtt aacaagttca
    12001 atacacggga ttgttataat tatttttgga aagttggtaa atcaaatgaa aaacaaatta
    12061 acaaaacttg aattcaagtg gatttgttac gacatgggaa actctgcctt tattttatta
    12121 gtcgcaacaa ttcttcctat ctattttaat tatctttcaa gctcacaagg tgtagccgaa
    12181 tacaactacc taagttattg gagctatgca gcaagtattt cgacattaat tgttgcactt
    12241 gcaggtccaa ttctaggaac acttgctgac tataaagatc acaaaaagaa aatcttctta
    12301 acttgtgcaa tgctaggcgc attagcatta gcatgtttct ggattccaag ttcttggttt
    12361 gcatttctag tcctatttat tgctgccaaa gttgcctatt ctttatcttt gatcgtatat
    12421 gactccatgc ttacagacat tactactgaa gatagaatgg atgccgtaag ttcaaaaggc
    12481 tatgcttggg ggtatatcgg tagtgttgtt ccatttatta ttagtttagt ctttgtatta
    12541 atgtatgaca aaatgggcat gacattaaag gcggccatga tgattgcctt tgttttaaat
    12601 gctgtatggt ggattgcatt tacacttcct ttagtaaaaa gctacaaaca gaaattctat
    12661 attgaaccag aagcacatcc tgttttagat acattcgctc gtttgaaaga cactttaatc
    12721 aaggcaaaag aacaaaagaa agtcttctta ttcttatttg cgttcttctt ctatattgat
    12781 ggtgtttata caattatcga tatggctaca gcctacggaa caagtttagg actaaataca
    12841 actggccttc tattagcttt attattgacg caaattgttg ccttccctgc aagtttaaca
    12901 ttcgctgtat tatccaaaac aaaagatacg gcttcattga ttaaggttgc gattatcgca
    12961 tacttcttaa ttgccttatt tgcggtacaa ttggataaac aatgggaatt ctgggtatta
    13021 gcagttgcgg ttggatgttt ccaaggtggt attcaagctt tatctagatc atactttgca
    13081 aaaatcattc cagaaaatgc cagtggtgaa tacttcggac tatttgatat ctgcggtaaa
    13141 ggtgctagct ttttaggaac tatgttgatt ggtgttgtaa cacagattac gggtaaacaa
    13201 aacttaggtg ttgccgctct ctccatcatg tttgtgattg gatacctaat ttttaataaa
    13261 gtatctaaaa tggacgacta aaaaagattg tacaagtaac gtacaatctt tttttaatcc
    13321 actttcatta tcattttaac aattccatac atggccgcat ataagacggc agcaacaggc
    13381 caaatcatat aggttacatc ccattgtttt gtccataagc tccaaccaag ataaacagct
    13441 gtcacaagac accaatatac acctgtgaat gtttccgttt tcttctttac aagcttttct
    13501 tttttcgtat aatcaccttc ttgcaacaat gtatcatagc tactttttat tatggaaaca
    13561 cgaatcagaa tattcacacc aacggaaacc aaaatgagta atacagctaa acataatgta
    13621 catatataat cagatgcttc catgcaacct gcaataatca acggaattac agataaaatg
    13681 catagaacaa caccacaagc taacgaaaag gagtaatgtt cttgatagct ttgattcttt
    13741 tctttgacaa gtcctgttac accatattct gtttcaaagg gttcttgttc taaatattgt
    13801 aaaggactca atttaattcc tgttttaata aatataaaca cggcaagtgc aaccatacct
    13861 aataatatgg cacacgcaat accaatggct aagctatcat tgaaagttga aactagaaac
    13921 aaagaaatcg ggcttaatat acacaaagtt gccgcattcg caagcatagg tgctgctttt
    13981 ttcttttcat tcatatatgc atttgcttct tccattgtga cacgatgtaa ttcacaatct
    14041 gtatcgcata ttgtctctgt attttctttt tcaacttcat cttttaacaa gtaatctgta
    14101 ctcactccaa ataaatcagc taactgaata atctttttta aatccggaat cgctcctgca
    14161 ctttcccatt ttgaaaccga ctgtctagat acgcctagtt tttgtgcaag atcttcttgt
    14221 gtccacccat tcttttttct ttcttcaatt attttatcag ctagaatcat tgttcttcct
    14281 ccttagccaa agtttactaa aatttgcggt tgcattctac caattagact tgtcaattta
    14341 tcaatttaca gttgcataga ctataaaaga cttactttaa cgcaagtctt tatctatgct
    14401 acatatttgc ttaatatatg attaagcttt tgttcatcaa tcggttttga gattgaatca
    14461 ttcataattg tttgatcaat actatattct gattgtgcac tcatggcgac aattggtata
    14521 gatggattga ttttacgaat gcatttagcc gcttcatccc cattcatttt tggcatcatg
    14581 atatccatta aaattaaaga gtattgattg ggttgttcct taactttttc aactgcttct
    14641 aatccattcc atgccttatc tacattagca tgtacggcat tcaagtaaaa ctcagccact
    14701 tccatattga tttcattatc ttctactaaa agaattcttt tatccttcaa atctaatttt
    14761 gaactatctt gttttccatt tttagatgta tccaattggt aagtaagaac gaccttgatt
    14821 tgtgtaccta cacccacttc acttttaact tcaatattgc caccaagctt atccactaaa
    14881 cccttcacaa tcgacatacc taagccagtt ccttcatatc ttgttctggc atcttgttta
    14941 gcttgtgaga aaggcgtaaa caagtgatct tcaatataat ctttactcat gccaatacct
    15001 gtgtctttga tttcaaattc aaatgtaact tttgaatcat cactctttat ttctctaaca
    15061 tacatatcaa tcgtgccatt tggtttgttg tacttaatgg cattcgttaa taggttaatg
    15121 agaattcttc taagttgtaa cgcatcccca attaaatgcg tatggactac attctccata
    15181 tgcttgtgat atgtaatatt ttgaagggat acttgggcat caatcaaact attcaattct
    15241 tcaaccatat aatttaaatc aaatggttca ttcacaatct ccatatgatc tgattctaat
    15301 ttagacatat tcaaaatatc ttcaaccaat tcatttaagt gttttgtcaa aacttccatc
    15361 ttatttaaag aatcatccaa cttatccata tcaccccagt ttttacgtat aatctgcatc
    15421 atacctaaaa taccattgat tggcgtacga atatcatggc tcatgcgatt cataaaatct
    15481 gttttcgctt tactttctaa atctactttt tgatacgcta attgtagctt ttccttttct
    15541 ctatcttctt tctcatgaaa tgccgatgta tttctaaata atagaatagc acgaactata
    15601 tttcgttcac tttgaatatc tatatcataa attatctctt cactcatcaa tatcatttct
    15661 tgaacccaaa tcactcgatc atctggcatt ttaatttgat attcaatagt aacatattta
    15721 tccccagcct tatagcgatc tagtaaattt tgacaagatt ccacaatccc gaaacttccc
    15781 aaacaatctt ctaatacatt ggatttccat tgtttaaagt atgcctcata tgaacaaggt
    15841 aaagcaggtc ttatattctc taccatatat ttatcataaa taccatcgat ttgattctgc
    15901 gttaaattca cagaaaaaat agattgcgcc atattcatta aggcttcaat ataattcgca
    15961 ttctctaaga tggattgttt caactgatca taataacgca tcaattgttt cttttcatcc
    16021 atctcaatcg tatcactata cattttaaag cccagcacaa aatggtgaag attaccatac
    16081 tcatcgctat ccaaatagac aaccatgatt ttacctttca attctgcatt attcatttga
    16141 tgataatatg gtatttcaaa tataggatta tcctttgtga catacttatt caaactattc
    16201 acatttaaaa catttcgata atccattctt ttatcttcat taaacaactc atttaaaatt
    16261 tgctcgattg ctcccgaata atttccatct tcagctaaaa agaaagaatg acttgcacgc
    16321 aaagtaaaat atgcgtctgt cttcgcatcg caataaatgc aatctgaaaa attgaatcct
    16381 ttttctttta attgttgaat cacatttaat attaaatttc gtttttgttt tgcttctttg
    16441 atttttgcct ggtttttatc aatatacata tttttatccg cctgacaaaa aagttcacgc
    16501 attgttaatt ttgaaaaatc atttgaatac gcaaaaccag ctgcataact taatggaatt
    16561 tctgaacatt tagcacattc ttttttgata ttttctaaat ttctttttac atcttccttt
    16621 gtcacatttt tataaaaagc aatgaattca tcaccgccac agcgacctac aaattgattt
    16681 tcatccacac ctttacgaag acttttcgca aacgaacgga tgtataaatc acctctttca
    16741 tgtccttgtt gattattgat gattctaaga ttattcaaat caaatacgca aattgccata
    16801 ttttgttcag cttctaaagt taaaatctct tcacacttat ttttatttgg caatcccgta
    16861 gcttcatcta aatatatttt gctttttaat tctcgattcg ccttcgcata acgcaaagct
    16921 ttaagtagtt catacaaaat cataaatact aagataacaa tatcaataac cgtcaaagtt
    16981 tcaaattgtt taagtcttgt cgcaattctt tgtgaatacg attccgcaag acctgtcgca
    17041 acatcacata agttaaaaaa tatttcactc ttttcaatta tacttgtatt ctcataacca
    17101 acttgtcgta caagatcaat ctcttgtttt aaagtatcaa aatacgcatc taattcttcc
    17161 atctttgctt gaaaagcctt atcatctaaa gatactaaat ctagttcttc actaccaaaa
    17221 cgaaggcctt ttatatagcc tttgacatcc gcaatcatat catcttgagg cattctggca
    17281 tcttctaatt taacaatacg ctgtgttttt ccacgtacta atcccgcata attgactaca
    17341 cgtgcggttc cttgaatacg tgaaaccata agtatgataa ttaaaatgag cacaattaaa
    17401 agactggtta aagccgtcat taaaatacgc attatttttg agaaacgatt ctttttctta
    17461 ttctgcatac atttattgta ttaagaaaat gaaattatca caagaaaata ataaaaggac
    17521 aaatcctaat aaaatggacc ctgtaaaacg gacacaataa aaaaagtaca ttagtaccta
    17581 gtcctgtttt acagggcttt tattttgtgt attatgataa
//
LOCUS       DBMDWL010000023        17134 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1131_length_17134_cov_3.280632, whole genome shotgun sequence.
ACCESSION   DBMDWL010000023 DBMDWL010000000
VERSION     DBMDWL010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 17134)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17134)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17134
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1131_length_17134_cov_3.280632"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            23..1117
                     /locus_tag="ACZPDN_02885"
     CDS             23..1117
                     /locus_tag="ACZPDN_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR1297599.1"
                     /translation="MTIKLYEQEGYQTTFHAHVISCVKKNKLYDIILDQTLFFPEEGG
                     QTCDKGTLNGIEVVDVQIINDEIHHYTHSALNGDVQGIIDWTHRYTNMQHHTGEHILS
                     GLIHKEFHVDNVGFHLGYNEITADYAMDLNNDQIKRIEALVNSVIFSNKPVKTYYLED
                     YSNIEYRAKLDLAKPRLVEIEDVDLCACCAPHVKSTIEVGLFKIIKSMKIKKGTRLFF
                     LCGKLAYEDYVLKHDQSIQISNQLSSNIEALSSNVQRILDENYALKQRIKQLNKEMVD
                     HLIIEHKENQIILCDELDRDTQLYYYNKLVSFSNNYVLLVSKQKDNYRFMTNSKDIFL
                     QLKERHQVRGGGKNDFFQGSLDTVNEQLFD"
     gene            complement(1122..1949)
                     /locus_tag="ACZPDN_02890"
     CDS             complement(1122..1949)
                     /locus_tag="ACZPDN_02890"
                     /inference="COORDINATES: protein motif:HMM:NF025497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HIR1297600.1"
                     /translation="MSVNIRIMQKGFFRRKKFIIDDLVKMSHLSFGVMDENCQLIPNQ
                     IGDHTILFDRKYLQRGIEIYIQNHDICLNLSLPTSMDEIQLFYYLVKVYCEYMDTNEF
                     VKDDWLMDIKDIDLQMVYDKRTSADALMDLKSKLSDHKYFEIFGILHPISIGENELND
                     FGTDLDLFGQYLHNQQALDAYYATPRIYNAHGRRIGMYALGANILTILPVEPYVVLNQ
                     IEGIEEWYVFMNDSLVKYRDLMSFIKKFDYYDANHRMVCLSKEEISEALNTLAIQKI"
     gene            complement(2003..2752)
                     /locus_tag="ACZPDN_02895"
     CDS             complement(2003..2752)
                     /locus_tag="ACZPDN_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013694.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR1297601.1"
                     /translation="MKIEVINEKRLNHIHYCRVHKENQQLSGSFWVPSKSKSKNKKMF
                     SFELNDSDFLVCDPEHIFKQKISGNKPSECILNLMNILIQEDMEFLQKLEMKLERIED
                     QLMSHTGSHYESQIFEMRKTISAFDSYYDQMIEVVQNLQEFYNDTHFETLEKRLTRLS
                     NVTDRLAEYSIQLREMHQTQVEMRQNQIMQFLTIVTTIFMPLTLITGWYGMNFRAMPE
                     LDSSYGYFIVIGVCILIIILEVIYFKKKKWF"
     gene            complement(2802..3197)
                     /locus_tag="ACZPDN_02900"
     CDS             complement(2802..3197)
                     /locus_tag="ACZPDN_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1297602.1"
                     /translation="MKYYPMFDLFDDFFTGNTTSDVMKTDIVEKDGNYEMSMELPGVK
                     KEDIQMELKDGYLKVSATHGSNTEDKDEKGRVVRKERVSGSYSRSFYVGEGIHQEDVK
                     ASFDNGELKIVVPKEAPKQVEENKFIPIE"
     gene            complement(3773..4978)
                     /locus_tag="ACZPDN_02905"
     CDS             complement(3773..4978)
                     /locus_tag="ACZPDN_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297603.1"
                     /translation="MIVERKEYLEKLIAWKDKKLIKIITGVRRCGKSMLLEIYQNYLK
                     QHNVEKEQIIAINFENLDYEELTDYKKLYNYLKEHLVTNKMTYIFLDEIQNVPDFPKV
                     LDSLYIKNNVDIYVTGSNAYMLSSEIATMISGRYVQMEMLPLSFREYMESTGSMNDRG
                     IKYAEYLQNSSFPYTLELKNQPNEIREYLEGIYNTIVVKDIVNRKKITDTMMLKSVLQ
                     FIFDNIGNPLSSKKIADTMTSAGRKIDTRTIEKYLEAFRESYIIYQTKRYDIKGKQYL
                     KTLEKYYIVDIGMRYMLLGSRQADAGHILENVVYLELLRRGYDVYVGKIGTFEVDFVA
                     KNSRGTIYYQVALTTHDESTLQRELRPLQVIRDHYPKIILTMDEEPEEQYDGIRRINA
                     RDWLLGLTE"
     gene            complement(5307..5486)
                     /locus_tag="ACZPDN_02910"
     CDS             complement(5307..5486)
                     /locus_tag="ACZPDN_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297604.1"
                     /translation="MKRIKAACITQTLCFFNHDQETTEYAKRAIVQEYEKYKSQLERS
                     GTKYKILSETTNVNG"
     gene            complement(5657..8866)
                     /gene="carB"
                     /locus_tag="ACZPDN_02915"
     CDS             complement(5657..8866)
                     /gene="carB"
                     /locus_tag="ACZPDN_02915"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1297605.1"
                     /translation="MPKRTDIKKILVIGSGPIIIGQAAEFDYAGSQACQSLREEGYEV
                     ILINSNPATIMTDTAIADRVYIEPLTVDFAKRVIYKERPDAILGSLGGQTGLNLVVEL
                     HEDGILDEFGVEILGTDLHAINRAEDRELFRSLMQEINEPVPESMIVHTVEEAVEFAK
                     REGYPLVVRPAYTLGGTGGGFADDEAELREICDSGLKASPVHQCLIEQSIAGYKEIEY
                     EVMRDAKDNAIVVCNMENIDPVGVHTGDSTVVAPVQTLTDRENQMLRNASLKIIRALK
                     ICGGCNVQLALNPTSFKYYVIEVNPRVSRSSALASKATGYPIAKISAKLAVGLGLDEI
                     INPITKTSYACFEPALDYIVTKFARFPFDKFPNADRHLGTQMKATGEIMSIGRNFEES
                     FLKAVRSLEMKVDHIYKKELAGLTKEELLEKIKKCDDERIFAVTQWLRDGYDIETIHN
                     VTKMDDFFLCHLSRIISLEKLMMENPKDVEVLRKLKKYGFADSYIARNWNMKEFDLYN
                     LRKENGIMPVYKMVDTCAGEFKSATPYFYSSYEHKNESEVTDRKKIVVLGSGPIRIGQ
                     GVEFDYATVHCVQTLREEGYEAIVINNNPETVSTDFSISDKLYFEPLTTEDVMHVIDL
                     EKPLGVIVQFGGQTAINLADSLVEHGVKILGTSLHSIDLAEDRHEFEAMLHKLDIPQP
                     KGETAVTVEESLVIANKIGYPVLVRPSYVLGGRAMEIVHNDDELRVYMATAVKEISHD
                     APILVDKYIVGKELEIDAIADGKNVYIPGVMEHIERAGIHSGDSISVYPTQIINEKVK
                     ETIIDYGTRIGEGFEFVGLYNIQFIVDKENNVYVLEVNPRSSRTVPFLSKITGVPMSQ
                     IATKCVLGESIEDQGLTPGYHPEDKERVFVKAPVFSFAKLRSVDTVLGPEMKSTGEAL
                     GSDVNLEKALYKALVASGINIPLHGSVLLTIDDDNKQDALEIAKRFYNIGYGIYATKG
                     TAKFLEDNGLFVHVAAKVNEDNGNETVLDIIRHGHVNFVINTMSNKDNSTSKDGFLIR
                     RVSAENNISCMTSLDTAHALIKVLESLSFSMISMNEMGK"
     gene            complement(8859..9938)
                     /gene="carA"
                     /locus_tag="ACZPDN_02920"
     CDS             complement(8859..9938)
                     /gene="carA"
                     /locus_tag="ACZPDN_02920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR1297606.1"
                     /translation="MMERLLILEDGSVYRGTAFGSDNFQIGELVFNTSMTGYQEILTD
                     NSYCGQIVMMTYPLIGNYGINRDDNESMIPCVFGFVVKDYCEYPSNFRCSETLDDFLK
                     RENIPGIYDIDTREITMKIRNVGTMKAVMANVDADVDVVVAGLKAADFLHDQVARVST
                     NKVFPIPNRGKKVVLMDFGAKLGIVRELSKRGCDLVIVPYDTPASTILSYRPDGVMLS
                     NGPGDPTDVKVAIETIKELIPQTVVFGICLGHQLISLACGAKTYKLKFGHRGANHPVV
                     NLSTGKVEITSQNHGFAVDIDSLKDTRLEMTHQALNDKSCEGVRHKDYPCFAVQYHPE
                     ASAGPEDSRYLFDQFIELMEENKNA"
     gene            complement(10202..10900)
                     /gene="pyrF"
                     /locus_tag="ACZPDN_02925"
     CDS             complement(10202..10900)
                     /gene="pyrF"
                     /locus_tag="ACZPDN_02925"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1297607.1"
                     /translation="MSKTIVALDFSSKEEVVDFLKQFDEPVYVKIGMELTYACGLEMV
                     KTVKEMGHHIFLDLKLHDIPNTVKGGMKNLANLGVDIVNVHCAGGIAMMKAAIDGLNE
                     GAKDGKRPMCIGVTQLTSTSKEAMNNELGIPGEVIDCVIKYAKNAKEAGLDGVVCSVH
                     EAKAIHEACGDDFLTVTPGIRLASDSKDDQKRVATPAFAKEQGCDYIVVGRSITKSAN
                     PVETYKEIEATMSK"
     gene            complement(10900..11808)
                     /locus_tag="ACZPDN_02930"
     CDS             complement(10900..11808)
                     /locus_tag="ACZPDN_02930"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009587563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1297608.1"
                     /translation="MDISVKLPGLNLKNPIIPASGCFGFGKEYAELYDLSVLGGIAIK
                     SATPQERFGNPTPRIAETPMGMLNAIGLQNKGVDSIIKNELPFLAQYDTEIMANVAGA
                     CEEDYVEVIKKLNDQPVIKAYELNISCPNVKHGGIGLGTKPELAAHVTKICKESATKP
                     VYVKLSPNVTDIVEIAKAVEEAGADGIVLINTLMGMRINLKTGKPLLANVTGGLSGPA
                     IKPVALRMVYQVAQAVNIPIIGVGGITCAEDVLEFLNAGASAVEVGAQNFVDPYVCPK
                     IIEDLPKVLEKYGYKNIEEAVGRSFK"
     gene            complement(11808..12551)
                     /locus_tag="ACZPDN_02935"
     CDS             complement(11808..12551)
                     /locus_tag="ACZPDN_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1297609.1"
                     /translation="MKWGNSMQEFAKILSNVEIAKDIWKMELKTDLAKTAKPGQFIEI
                     TVPGFYLCRPISISEIKEDVLVIIYKVLGEGTLKMTELEGKLDIFGPLGNGFPIEDKE
                     EVLLIGGGVGVPPLYETAKQYLSKNARVTVVLGANDADSVFYVDEFKALGCDVYVATM
                     DGSLGTKGTALDAIKENNVTCNFVLSCGPLPMLKAINEQYSEGYISLEARMACGLGAC
                     MGCVVKDVEGHSLRVCKDGPVFEMGKVAL"
     gene            complement(12583..12846)
                     /locus_tag="ACZPDN_02940"
     CDS             complement(12583..12846)
                     /locus_tag="ACZPDN_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014225.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297610.1"
                     /translation="MEKQYGKDKTYLFEAEGLHVHRASKIETEDGKNVLDIIRNGRVN
                     FVINTVSNKKNISADGFLIRRVSAENNISCMTSLDTANALAKV"
     gene            complement(12821..14086)
                     /locus_tag="ACZPDN_02945"
     CDS             complement(12821..14086)
                     /locus_tag="ACZPDN_02945"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1297611.1"
                     /translation="MRLIKNGQVLIDNQVEKKDILMDEGKIIQIDDSILKDCEVIDAA
                     GLTVIPGLVDVHVHFREPGHTEKETIHTGSLAAAHGGFTSVFAMPNVIPFPDDVDTMK
                     DYQQLIEKESVVHTYPYACITLEEASKEVVDMKAIKELGIRWFSDDGVGVATSEIMKE
                     AMEKAKENDCMIVAHTEDMSYRRPGACVHDSEVVTSKGWLGIPSACESAQLIRDLEIT
                     KEVGNAYHACHISAKESVEALKDAKESGSNVSAEVTVHHLLLEDKDVVGPNWKMNPPL
                     RSHEDRMSLIEGLENGTLDCIANDHAPHTYEEKKRSMDKAPFGIVSLESAFALLYTEF
                     VHRQKRWTLAQLVNWMSAAPAKRFGLENVGQIKEGYNADLVLVDLEHESLIDIDTFES
                     MGKNTPFNGWSVNAQIKETIVDGKTVWKG"
     gene            complement(14089..14991)
                     /locus_tag="ACZPDN_02950"
     CDS             complement(14089..14991)
                     /locus_tag="ACZPDN_02950"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR1297612.1"
                     /translation="MNKNSLLSMRDLSVEDIYSILDDARLFNSSFKDWQLPMQKALVA
                     NLFFEASTRTHFSFESAQHQLGCQVADFMAGASSVTKGETLYDTVKTFESIGYDMVVI
                     RHPQDEYFKELENINIPILNAGDGAGNHPTQCLLDLLTMQNEFGTLEGLNVVICGDIL
                     HSRVAASDKEALERLGSTVRFAGPKEWERPGYPTIDFDEAIEWADVVMMLRIQKERGS
                     GMAGMTDSEYLEKYGLTKERYARMKDKAIVMHPAPVNRGVEIDTDLVEAEKSRIFEQM
                     HNGMLVRKAVIKRGFGFAPFKEEK"
     gene            complement(15149..15526)
                     /locus_tag="ACZPDN_02955"
     CDS             complement(15149..15526)
                     /locus_tag="ACZPDN_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1297613.1"
                     /translation="MKQQISSTKAPKALGPYSAGVLTEGLVFLSGQLGVDTNGNLVSE
                     NVVEQTVKAFENIGVLLETSGLIYDDIVKTTVLLDDMNDFAKVNEEYAKYFTEPFPAR
                     SCFEVAKLPKNAKVEIECIAIKK"
     gene            complement(15537..16160)
                     /locus_tag="ACZPDN_02960"
     CDS             complement(15537..16160)
                     /locus_tag="ACZPDN_02960"
                     /inference="COORDINATES: protein motif:HMM:NF027790.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5052 family protein"
                     /protein_id="HIR1297614.1"
                     /translation="MNKNIKRVSVVCLCLLLSGCSMFQSALNDIKGNLVGNGYTIYTY
                     DNYGSEVMETSGDKISITGNAVESTSYDENGSVVRGYELSSVITITVDGKEIESCGDT
                     CIFEQKGLTPDVDFTQADIDSNTHGQISDNTSVARFINKYKNMFGKGRIVVIKSQLGQ
                     PIAAYSGDKVYWKIPDNLPKMTKLMIDGKALYIHRANFQIIDKELLD"
     gene            complement(16187..16396)
                     /locus_tag="ACZPDN_02965"
     CDS             complement(16187..16396)
                     /locus_tag="ACZPDN_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297615.1"
                     /translation="MECSKQAFLACKEICCLIQDMEWLDLKELEEKEKIEMYQKMMDK
                     EFGIKAIAFIFSVSEESIKKLLMKA"
     gene            complement(16478..>17134)
                     /locus_tag="ACZPDN_02970"
     CDS             complement(16478..>17134)
                     /locus_tag="ACZPDN_02970"
                     /inference="COORDINATES: protein motif:HMM:NF016629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1297616.1"
                     /translation="SMVVRAATYEYVAYDRMLKKLKKNKAKEKLPMVHILVFYTGEKP
                     WNAANKLSQLVEVDERFESYFHDYKMNLIEITGNTSYNFNEEDVYNLFYICRSIYDQS
                     IYEGKSNSFGLVKSSVLKVVKTLTDVEWLDLEELEEKEEIEMCEAEKKWLEVKSKEWE
                     AEGIRKGIEQGIEQGIEQGVELGQVLLYKTMIKNGMSVNEISKVCSISVENLKQVLSN
                     "
BASE COUNT         5264 a         3632 c         2268 g         5970 t
ORIGIN      
        1 atgattaatt ccggtgatat atatgacaat taaactatac gaacaagaag gctaccaaac
       61 aacatttcat gcccatgtta tttcatgtgt taaaaagaat aaattatatg acataatctt
      121 ggatcaaacc cttttctttc ctgaagaagg tggacaaaca tgtgacaaag gaacgttgaa
      181 tggtattgaa gtagtggatg tgcaaatcat aaatgatgaa atccatcatt atacgcactc
      241 tgcattaaat ggtgatgtac aaggtattat tgactggact catcgctaca ccaatatgca
      301 acatcataca ggagaacata ttctttctgg attgatacac aaagaatttc atgtggataa
      361 tgtcggattt catttaggat acaatgaaat tacagctgat tatgcgatgg acttaaataa
      421 tgatcaaatt aaaagaattg aagctttagt gaattctgtc atcttttcaa ataaacctgt
      481 taaaacctat tatttagagg attattccaa cattgaatat cgtgcaaaac ttgacttagc
      541 caaaccacgc cttgtcgaaa ttgaagatgt agatttatgc gcatgttgcg caccccatgt
      601 aaaaagtacg attgaagtgg gattgtttaa aatcattaaa tcgatgaaaa tcaaaaaagg
      661 cacacgccta ttcttcttat gcggaaaact tgcctatgaa gactatgtct taaaacatga
      721 tcagtctatt caaatctcaa atcaattgtc ttcaaacatt gaagctttat catccaatgt
      781 acaacgcata ttagatgaga attatgcttt gaaacaaaga atcaaacaat taaataaaga
      841 gatggttgat cacttaatca ttgaacataa agaaaaccaa atcattttat gtgatgaatt
      901 ggatcgagat acacaattgt attactataa taagcttgta tcttttagca acaactatgt
      961 tttacttgtt tctaaacaaa aagataatta tcggttcatg acaaattcta aagacatatt
     1021 cttacaactc aaagaaagac atcaagttcg tggtggagga aaaaatgatt tctttcaagg
     1081 atctctagat acagttaatg agcagctatt tgattagctg ctcatatttt ttgtatagcg
     1141 agtgtattta aagcctctga aatctcttct ttcgataaac ataccattct atgattggca
     1201 tcataataat caaacttttt aataaaactc attagatcac gatacttcac aaggctgtca
     1261 ttcataaata cataccactc ttctattccc tcaatttgat tcaatacaac atatggctct
     1321 actggaagaa tcgttagtat attcgctcct aatgcataca taccaattct tctaccatgt
     1381 gcattataga ttcttggcgt ggcataatat gcatccaaag cttgttgatt atgtagatat
     1441 tgaccaaaca aatctaaatc tgtaccaaaa tcattcaatt cattttcccc aattgaaatc
     1501 ggatgaagaa tgccaaagat ttcaaaatac ttatgatccg ataatttact cttcaagtcc
     1561 ataagtgcat cggcgctcgt tcttttatca tacaccatct gtaagtcaat atcctttatg
     1621 tccattagcc aatcgtcttt cacaaattca ttggtatcca tatattcaca atatactttt
     1681 actaaataat aaaacaactg aatttcatcc atacttgtag gaagacttaa attcaaacaa
     1741 atatcatgat tttgaatata gatttcaatc cctctttgaa gatatttacg atcaaacaag
     1801 atcgtatgat ctccaatttg atttgggatc aattgacagt tttcatccat aaccccaaaa
     1861 gataagtgag acattttaac taaatcatcg attataaact tttttctacg aaagaaaccc
     1921 ttctgcataa tacgaatatt cacagacata caaacacccg ctctctacca aaatcataca
     1981 ctgaaacaag cgttttttat agctaaaacc attttttctt tttaaagtaa atcacttcta
     2041 aaataatgat caatatacaa actccaatca caatgaaata tccataagaa ctatctaatt
     2101 ctggcatagc cctaaaattc ataccgtacc atccagtaat taaagtgagt ggcatgaata
     2161 tcgttgtcac aatcgttaaa aactgcatga tttgattctg acgcatctcc acttgagttt
     2221 gatgcatttc acgaagctgt atagaatatt ctgccaagcg atccgtaaca ttcgataaac
     2281 gtgttagtct tttctctagt gtttcaaaat gtgtatcatt ataaaattcc tgcaagtttt
     2341 gtaccacttc aatcatctga tcataatacg aatcaaaagc acttatcgtt ttcctcattt
     2401 caaagatttg cgactcataa tgagatcccg tatgcgacat taactgatct tctattcttt
     2461 caagtttcat ttctaatttt tgaagaaact ccatatcctc ttgaatcaat atattcatca
     2521 aatttagaat acattcacta ggtttattcc ctgaaatctt ttgtttaaaa atatgttctg
     2581 gatcgcaaac caaaaaatca ctatcattca attcaaaact aaacatcttc ttattcttag
     2641 attttgattt tgaaggaacc caaaaagaac ccgatagttg ctgattctct ttatgtaccc
     2701 tacaataatg aatatgattt aaacgctttt catttattac ctctatcttc ataatttccc
     2761 cctttaaaaa aagaatacac catataggtg cattcttgta gttattcaat tggaataaat
     2821 ttattttctt cgacttgttt aggtgcttcc ttaggaacaa cgatcttcaa ttcaccattg
     2881 tcaaaggaag ctttgacatc ttcctgatga ataccttcac caacatagaa gcttcttgaa
     2941 taagatccac taacacgttc tttacgaaca acacgacctt tttcatcctt gtcttctgtg
     3001 ttcgaaccat gtgtagcact gacctttaaa tatccatcct tcaattccat ctggatatct
     3061 tcctttttaa ctccaggaag ttccatactc atttcatagt ttccatcttt ttccacaata
     3121 tctgtcttca tgacatcact ggttgtgttt cctgtaaaga aatcatcaaa taaatcaaac
     3181 attggatagt acttcataaa tatcgcctcc tgatttctag attatgaaat ctatcttcaa
     3241 gttacgggat atcttttgac ttatctctaa cttcaattct tattatagtc atctttttag
     3301 cactgtcaac aattaagtgc taattttttt acaaaaatta gagtgcgatt tctcacacct
     3361 taactttgca gaatttgtac aactaaaaaa taaaagaagc attaatagtt aaatttacca
     3421 atgcttctgt caacatattt ttcatccata tcaaatcgtt ttaatatcaa tttctatttt
     3481 gctgttaatg catattttct tctgtattta ccaacactgt ttcttgcaca ttcgatattt
     3541 tcaagctctg atatcattcc tagaagcgta tatgctttac ggttcttttt ttcttagtac
     3601 ttctgctttc tggaacagct cattccttac gatcaaccat ttattcaaaa attcaattgt
     3661 agttatacat gttttacaaa gttaaaaagt gttcttagac tgcattataa attctcgagt
     3721 gtttgactta tcatgataaa ttgctacatt gttttcctgt tatttcctat acttactccg
     3781 ttaaccccaa cagccagtct cttgcattga ttcgcctaat tccatcgtat tgttcttccg
     3841 gctcctcatc catcgtcagg attatctttg gataatgatc tctaattacc tgcaatggac
     3901 gaagttctct ttgaagtgta ctttcgtcat gtgtcgtcaa tgccacctga taatatattg
     3961 tccctctgct attttttgct acaaaatcca cttcaaaagt accgattttt ccaacataca
     4021 catcgtatcc tcgccgcaac aattctaaat aaacaacatt ttccaaaata tgtccagcat
     4081 ctgcctgcct tgaccctaaa agcatatagc gcattccaat atcaacaata taatactttt
     4141 caagtgtttt cagatattgt tttcctttga tatcatacct tttcgtctga tagatgatat
     4201 aactttcgcg gaaagcctcc aaatattttt ctatcgttct tgtatcaatc tttcttcctg
     4261 ctgatgtcat cgtatctgca attttttttg atgacaatgg gtttcctata ttatcaaaaa
     4321 taaactgtag aacacttttt agcatcatag tatctgttat ttttttccta tttacaatat
     4381 cttttacaac aatcgtgtta tagattcctt ccagatattc cctaatttca tttggttgat
     4441 tttttaattc caatgtatag ggaaaagaac tattttgcag atattccgca tattttattc
     4501 ctctgtcatt catacttcct gtactttcca tatattcacg aaatgataat ggaagcatct
     4561 ccatctgtac atatcttcca gaaatcatcg ttgcaatctc actggatagc atataagcat
     4621 tcgaacctgt aacataaata tctacattgt tctttatata taaactgtct aatacttttg
     4681 gaaaatctgg cacgttctgt atttcatcca gaaaaatata tgtcatttta tttgtcacca
     4741 aatgttcttt caaataatta tatagttttt tataatccgt aagttcctca taatcaagat
     4801 tttcaaaatt aatcgcaata atctgttctt tttctacatt atgttgcttc agataatttt
     4861 gataaatttc caataacatt gatttaccac atcgccttac tcctgtaata attttaatta
     4921 attttttatc tttccaggca attaattttt caagatattc ttttctttca acaatcacac
     4981 gatcacccca cattcggttt atatattata atccaaaaaa gtaataggac acaatagttt
     5041 tttacatcac aatgcaataa ctatttattt ttcatgtttt tttgcattca ttatctcttc
     5101 acgaaaaaag gagagttccg aactctctgc ctgatattct gggaaaactt ttgtttccgt
     5161 catcattcca tatctatttg acatttaatt tgatgtattg ctgactatat ctataatttc
     5221 ccaacaggta tatcctataa tcacacgcca tcttctattg tttctgccat ctgctcaccg
     5281 aatttaaaat tgagattcac aattagttaa ccatttacat ttgtagtttc ggataaaatt
     5341 ttatattttg tgccacttct ttccaactgt gatttatact tctcatactc ctgtacaatc
     5401 gctctttttg catactctgt cgtttcttga tcatgattga agaaacacaa tgtttgagtg
     5461 atacaagctg ctttaattct tttcattctc gctacctctc cttcatttta cagatacatt
     5521 acattaaaag ggatcatccc actcaataaa tttgaatgaa atgatccctg attctcatgt
     5581 aaattgtact tggtttttac acaattatta tacttaattt ttaaatgtgt aaaaataaag
     5641 tgtgatttct cacactctat ttacccattt cattcataga aatcattgag aaactcaaag
     5701 attccaatac tttaattaag gcatgtgccg tatctaaact tgtcatacaa gaaatgttat
     5761 tttctgcaga cacacgacga attaagaatc catctttact tgtagaatta tctttatttg
     5821 acattgtgtt gatgacaaag tttacatgtc catgacgaat aatatccaat accgtttcat
     5881 ttccattatc ctcattgact ttggctgcta catgtacaaa taagccatta tcttctaaga
     5941 atttagctgt acccttcgtc gcataaattc catatccaat gttatagaaa cgttttgcga
     6001 tttctaaagc atcctgttta ttatcatcat caattgttag taatacactt ccatgtaaag
     6061 gaatattgat tccagaagct actaaagctt tgtacaatgc tttttctaag ttgacatcac
     6121 tacccaaagc ctcacctgta gatttcattt caggtcctaa aactgtatct acactacgta
     6181 atttcgcaaa tgagaatact ggtgctttga caaagactct ttctttatct tcaggatgat
     6241 atcctggtgt taagccttga tcttcaatac tttcacctaa tacacactta gtcgcaattt
     6301 gagacatagg aacaccagta atcttagata agaatggtac ggtacgagag gaacgtgggt
     6361 ttacctctaa tacatataca ttgttttctt tatctacaat gaactgaatg ttgtataaac
     6421 caacaaattc aaagccttca ccaattcttg ttccataatc aatgattgtt tctttcacct
     6481 tctcattgat gatctgtgta ggatatacac taattgagtc accagagtga ataccagcac
     6541 gttcgatatg ttccattaca cctggaatat atacattctt tccatccgca atcgcatcga
     6601 tttcaagttc tttaccaaca atatatttat caactaaaat tggagcatca tgactgattt
     6661 cctttacggc tgttgccata tagactctta attcatcatc attatgtacg atttccattg
     6721 cacgaccacc caacacataa cttggacgta ctagtactgg ataaccaatc ttattagcga
     6781 ttactagtga ttcttcaaca gtgactgcag tttcaccttt tggttgagga atatccaatt
     6841 tgtgtaacat tgcctcaaat tcatgacggt cttccgctaa atcaatagag tgtagtgaag
     6901 tacctagaat cttaactcca tgttctacca atgaatcggc taagttgatt gcggtttgtc
     6961 caccaaattg aacaataaca cccaatggtt tttctaaatc aattacgtgc attacatctt
     7021 ctgtagttag tggttcaaaa tataatttgt cactaattga gaaatcggtt gatacagttt
     7081 cagggttgtt gttgatgacg atagcttcat aaccttcttc acgcaaagtc tgtacacaat
     7141 gtactgtcgc atagtcaaac tcaacacctt gtccaatacg aattggtcct gaacctaata
     7201 caacgatttt tttacggtct gttacctcag attcgttctt atgttcataa cttgaataga
     7261 agtatggagt tgcactctta aactcacctg cacaagtatc taccatctta tatactggca
     7321 tgattccatt ttctttacgc aagttatata aatcaaattc tttcatattc cagttacgtg
     7381 caatgtagct atccgcaaaa ccatatttct ttaatttacg taaaacttca acatctttgg
     7441 gattttccat cattagcttt tctaagctga taattcttga caagtgacac aagaagaaat
     7501 catccatttt cgtcacatta tgaatggttt ctatatcata tccatcgcga agccattgtg
     7561 taaccgcaaa aattctttca tcatcacact tcttgatttt ttcaagtaat tcttctttcg
     7621 ttaatccagc caattctttt ttgtatatat gatccacttt catctctaaa gaacgaactg
     7681 cttttaagaa agattcttca aagttacgtc caatggacat aatttcacca gtcgccttca
     7741 tttgtgttcc taaatgacga tccgcattag ggaatttatc aaatgggaaa cgtgcaaatt
     7801 tcgttacaat atagtctaat gcaggttcaa aacatgcata acttgtttta gtaattgggt
     7861 taataatttc atctaatcct aaaccaaccg ctaatttagc actaatcttc gcaattggat
     7921 aacctgttgc cttactagct aatgcagaag aacgagatac acgagggtta acttcgataa
     7981 cataatattt aaaacttgtt ggattcaatg ccaactgtac gttacatcct ccacaaattt
     8041 ttaatgcacg aataattttc aaagatgcat tacgaagcat ttggttttct cggtctgtca
     8101 aagtttgaac tggcgcaaca accgtactat ctccagtatg cacacctact ggatcaatat
     8161 tttccatatt acatacgacg atcgcattgt ctttggcatc acgcattact tcatattcaa
     8221 tttctttata tccggcaata ctttgttcaa tcaaacattg atgaactgga ctagccttta
     8281 atccagaatc acaaatttca cgaagttcag cctcatcatc ggcgaaacct ccacccgttc
     8341 caccaagtgt ataagctgga cgaacaacta atggataccc ttcgcgtttt gcgaattcaa
     8401 cagcttcttc tacggtatga acaatcatac tttctggtac tggctcattg atttcctgca
     8461 ttaaagaacg gaacaattca cgatcttccg cacgattgat cgcatgtaag tctgtaccta
     8521 aaatttctac accaaactca tctaagattc catcttcatg taattctaca actaagttta
     8581 atcctgtttg accacctaat gatcctaaaa tggcatcggg tctttcttta taaataactc
     8641 gttttgcgaa atcaacagtc aaaggctcaa tataaacacg gtctgcgatg gctgtatctg
     8701 tcatgattgt ggcaggattt gagttaatta agatgacttc atatccttct tcacgaaggg
     8761 attgacatgc ctgacttccg gcataatcaa attcagctgc ctgaccaatg ataattggac
     8821 cagaaccgat cactaagatt tttttgatat ctgtacgttt aggcattttt attctcctcc
     8881 attaattcaa taaattgatc aaacaagtaa cgagaatctt caggtcctgc acttgcttct
     8941 ggatgatatt gcaccgcaaa gcaaggatag tctttatggc gaacaccttc acaactctta
     9001 tcatttaatg cttggtgtgt catttccaag cgcgtatcct ttaagctatc aatatctacc
     9061 gcaaaaccat ggttttgaga agtaatttct actttacctg ttgaaagatt aaccactgga
     9121 tggttagctc cacggtgtcc aaatttcaat ttatatgttt ttgcaccaca agccaaagaa
     9181 atcaactggt gacctaaaca aattccaaat acaaccgttt gaggaatcaa ttctttaatc
     9241 gtttcaatag cgacttttac atccgtagga tctccaggac cattcgacaa cataacgcca
     9301 tctggacgat aagataggat tgtgcttgct ggtgtatcat atggaacaat gactaaatcg
     9361 catccacgtt ttgatagttc acgaacaata cctaattttg ccccaaaatc catcaataca
     9421 actttcttgc cacgatttgg gattgggaat actttatttg ttgaaacacg agccacttga
     9481 tcatgtaaga aatcagcggc ttttaaacca gcaactacca catctacatc ggcatccaca
     9541 ttagccataa cagccttcat ggtacctaca ttacgaatct tcattgtgat ttcacgtgta
     9601 tcaatatcat aaatacctgg aatattttcg cgttttaaga aatcatctaa tgtttctgaa
     9661 catctgaaat tgcttggata ttcacaatag tctttaacta caaatccaaa tacacatgga
     9721 atcatacttt cattatcatc acgattgatt ccatagttac caatcaatgg atatgtcatc
     9781 attacaattt gtccacaata tgaattatct gtcaaaattt cttgataacc cgtcattgac
     9841 gtattaaata ctaattcacc aatttggaag ttgtcactac caaacgctgt tccgcgatat
     9901 acacttccat cttctaaaat caatagtctt tccataattt gtcctttctg ttatttactc
     9961 aaaaaaaaga ttagcaaaca atgcattgcc aatctttaaa tggaactatt aaaaagtata
    10021 gcactacgaa gaatactatt catctgaatc actgatgttt tgttcacaaa taaagctaga
    10081 tttttgatca tacgtatcct ccttttgaaa aaaatactat atttttcctg cttagtatac
    10141 taccacaaat cataaaaatt tcaataaaaa agtgtaaaaa aaagatgtga tttctcacac
    10201 cttattttga catagttgct tcaatttcct tatatgtttc aactgggtta gcactcttcg
    10261 taattgaacg accaactacg atataatcac agccttgttc tttcgcaaat gcaggagtcg
    10321 caacacgctt ttgatcatcc ttagaatcac ttgccaaacg aatacctgga gttactgtta
    10381 agaagtcatc tccacaagct tcatgaattg ccttagcttc atgtacggaa catacaacac
    10441 cgtctaagcc tgcttctttc gcattcttcg catatttaat aacacaatca ataacttcac
    10501 caggaattcc caattcgtta ttcatagctt ctttagaagt gcttgtaagc tgagttacac
    10561 caatacacat tgggcgcttt ccatctttag ccccttcatt caagccatca atggcggcct
    10621 tcatcatcgc aataccacca gcacagtgta cattgacaat atctacacct aagttcgcta
    10681 agtttttcat tccaccctta actgtgtttg gaatatcatg caatttcaag tctaagaaaa
    10741 tgtggtgacc catttccttc actgttttta ccatttctaa accacacgca tatgttaatt
    10801 ccataccaat ctttacatat acgggttcat caaattgttt taagaaatct acaacttctt
    10861 ctttactcga aaaatctaat gctacaattg ttttactcat tatttaaaac tccttccaac
    10921 tgcttcttca atatttttat atccatattt ctctaatact tttggaagat cctcaatgat
    10981 ttttggacat acataaggat ccacaaagtt ttgggcaccc acttcaacag cacttgcccc
    11041 cgcgtttaag aattccaata catcttctgc acaagtaatt ccaccaacac caatgattgg
    11101 aatatttaca gcttgtgcta cttgatatac cattcttaag gcaaccggct taatggcagg
    11161 accagaaaga ccacctgtta cattggcaag caatggtttt cccgtcttca aattgatacg
    11221 catacccatt aaagtattga tcaatacaat accatctgca ccggcttctt caacggcttt
    11281 ggcaatttct acaatatccg ttacattagg tgataacttc acatatacag gttttgtagc
    11341 ggattcctta caaatcttag tcacatgtgc tgcaagttca ggtttcgttc ccaaaccaat
    11401 accaccatgt tttacattag gacaagaaat atttaattca taagccttaa ttactggctg
    11461 atcatttaat ttcttgataa cctctacata atcttcttca catgcacccg ccacattggc
    11521 catgatttct gtatcatact gtgcaaggaa tggaagttca tttttgataa tagaatctac
    11581 acctttattt tgtagaccaa tcgcattcaa cattcccatt ggagtttcgg caattcttgg
    11641 tgtaggattt ccaaaacgct cttgtggtgt agccgatttg atggcaatac cacctaatac
    11701 gctcaaatca tatagttcgg catattcttt accaaatcca aaacatccac tagctggaat
    11761 aattggattc ttcaaattca atccgggtag ttttactgaa atatccatta taaagccacc
    11821 tttcccattt caaatacagg accatcttta catacacgca atgaatgtcc ttctacatct
    11881 ttcacaacac atcccataca agcaccaagg ccacatgcca tacgagcttc taatgaaata
    11941 tagccttcac tatattgttc gttgattgcc ttcaacatag gtaaaggtcc acaactaagt
    12001 acaaagttac atgtcacatt gttttctttg atcgcatcca atgctgtacc ctttgttcct
    12061 aatgaaccat ccattgttgc gacatataca tcacacccaa gcgctttgaa ttcatccaca
    12121 tagaatacgc tatctgcatc atttgcacct aatactaccg ttacacgtgc atttttactt
    12181 aaatattgtt ttgcggtttc atatagtggt ggtacaccaa cacctccacc aatcaacaac
    12241 acttcttctt tatcttcaat cggaaaacca tttccaagtg gaccaaagat atctaatttt
    12301 ccttcaagtt cagtcatttt taaagtacct tcaccaagca ctttgtagat aatgactaaa
    12361 acatcttcct taatttcact aatcgagata ggtctacaca aatagaaacc tggaactgtg
    12421 atttcaatga attgaccagg cttagctgtt ttagctaagt ctgttttcaa ttccattttc
    12481 caaatatctt ttgcgatttc gacattcgat agaatcttcg caaattcctg catactattt
    12541 ccccatttca ttcatagaaa ccattgagaa actcaatggc tcttaaactt ttgctaatgc
    12601 attcgctgta tctaaacttg tcatacaaga aatgttattt tctgcagaca cacgacgaat
    12661 caagaatcca tcagcactta tgttcttctt attagacact gtattaatga caaagtttac
    12721 acgtccatta cgaataatat ctagaacgtt tttaccatct tctgtttcaa tcttactggc
    12781 acgatgaaca tgcaatcctt cagcttcaaa taagtatgtt ttatcctttc catactgttt
    12841 ttccatctac aatggtttcc tttatttgcg catttacgct ccaaccatta aatggcgtat
    12901 ttttacccat agattcaaat gtatctatgt cgatcaaaga ttcatgttct aaatctacta
    12961 agactaaatc ggcattatat ccttctttaa tttgtcctac attttctaag ccaaaacgct
    13021 tagcaggtgc agccgacatc caattcacta attgagctaa tgtccaacgt ttttgtctat
    13081 gcacaaattc agtgtatagt aacgcaaacg cactttctaa tgaaacaata ccaaatggcg
    13141 ctttatccat cgaacgtttc ttttcttcat aagtatgtgg agcatggtca ttggcaatgc
    13201 aatccaatgt tccattttct aagccttcaa ttaaagacat acgatcttca tgacttctta
    13261 atggtggatt cattttccag tttggtccga caacatcttt atcttctaat aataaatgat
    13321 gtactgtaac ttctgcactt acattcgaac ctgattcttt tgcgtctttt aatgcttcta
    13381 cactttcctt agccgaaata tggcaggcat gatacgcatt tcctacttct tttgtgattt
    13441 ctaaatcacg aatcaactgt gcgctttcac aagcacttgg aattccaagc cagcccttag
    13501 atgtcacaac ttcactgtca tgtacacaag ccccaggtct acgatagctc atatcttctg
    13561 tgtgggctac gatcatacag tcattttctt tcgccttttc catagcttcc ttcatgattt
    13621 cagatgtggc tacccctaca ccatcatcac taaaccaacg aatacctaat tctttaatag
    13681 ctttcatatc cactacttct ttactcgctt cttctaaagt gatgcaagca tatggatatg
    13741 tatgtacaac agattctttt tcaatcaatt gttgatagtc tttcattgta tctacatcat
    13801 ctggaaaagg gatgacgttt ggcatcgcaa acacagaagt aaatcctcca tgtgctgctg
    13861 ctaaactacc tgtatgaata gtttcttttt ctgtgtgtcc tggttctcta aaatgtacgt
    13921 gtacatccac taaccctgga atgactgtaa gacctgcagc atcaataact tcacagtctt
    13981 tcaaaataga atcatcaatc tgaatgatct ttccttcatc cattaaaata tctttctttt
    14041 ctacttgatt gtcaattaaa acttgaccat tttttattaa acgcatactt atttttcctc
    14101 tttgaatgga gcaaaaccaa atccacgttt aataacggcc tttctaacca acataccatt
    14161 gtgcatttgt tcaaaaattc ttgatttttc agcttccact aaatccgtgt caatttcaac
    14221 tccacgatta acaggagctg gatgcattac gatagctttg tctttcattc ttgcataacg
    14281 ctcttttgta agaccatatt tttctagata ttcactatct gtcatacctg ccattccaga
    14341 acctctttct ttctggatac gaagcatcat gacaacgtct gcccattcga tggcttcatc
    14401 aaaatcaatt gttggatagc ctggtctttc ccattcttta ggtccggcaa agcgaacagt
    14461 cgaccctaat ctttctaagg cttctttatc actggcagcc actcttgaat gtaatatatc
    14521 tccacagatt actacattta agccttctag tgtaccaaat tcattttgca tagttagtaa
    14581 gtctaacaag cattgtgttg gatggtttcc tgcaccatct ccagcattta aaattggaat
    14641 attgatattt tccaattcct tgaaatattc atcttgagga tgacggatca ccaccatgtc
    14701 atacccaata gattcaaatg tctttactgt atcataaaga gtttctccct ttgtgacact
    14761 agatgctcct gccataaaat cggctacttg gcatcctaat tggtgctgtg cactttcgaa
    14821 tgagaaatgt gttcgagttg aagcctcaaa aaaaagatta gcaaccaatg ctttctgcat
    14881 tggtaattgc caatctttaa aggaactatt aaaaagtcta gcatcatcaa gaatgctata
    14941 aatgtcttca actgacagat ctctcatgga taataacgaa tttttgttca tataatcctc
    15001 cttttgactt taaaaaaatg gatttaaaaa gtcccttttc aaatccaatt tctctttcat
    15061 atatactacc ataaatcatg gatttttcaa caataaaatt cttcaataca tcaaagaacg
    15121 aactctattt acgacatatg tttttaaact attttttgat tgcgatacac tctatttcaa
    15181 ccttagcatt cttaggtaat ttagccactt caaaacaact tcttgcaggg aacggttcag
    15241 taaaatattt cgcatattct tcatttactt tcgcaaagtc gttcatatca tccaataata
    15301 cagttgtctt gacaatatca tcataaatta agcctgaagt ttcgagtaat accccaatat
    15361 tttcaaaggc ctttacagtt tgttctacga cattttcact gactaaattc ccattggtat
    15421 ctacgcctaa ttgtccagat aagaatacta atccttctgt taaaactcct gctgaataag
    15481 gacctaatgc ctttggtgct tttgtactac taatttgttg tttcattttg tatctcctaa
    15541 tctaataatt ctttatcaat gatttgaaaa ttcgctctat gaatatacaa agccttccca
    15601 tcaatcataa gcttcgtcat ttttggtaaa ttatctggaa tcttccaata caccttatct
    15661 ccagagtagg cggcaattgg ttgtcccaat tgtgatttaa tgactacaat tcttccttta
    15721 ccaaacatat ttttatactt gttgataaaa cgagcaacag atgtattgtc cgatatttgt
    15781 ccatgtgtat ttgaatcaat atctgcttgt gtaaaatcca catctggtgt taagcccttt
    15841 tgttcaaaaa tacaagtatc tccacatgat tctatttctt ttccgtctac cgttatcgta
    15901 attacacttg ataattcata tccacgaaca acactaccat tttcatcata agaagtagac
    15961 tctaccgcat tgcctgtaat actaatttta tctccacttg tctccatgac ttcactgcca
    16021 taattatcat acgtatatat cgtatatccg ttacctacta aattaccctt gatatcatta
    16081 agagcagatt gaaacatgct acaaccactc aacaataaac ataaacatac tacacttact
    16141 ctttttatat tcttattcat atttcccccg ttttataatg cattttctaa gctttcatca
    16201 atagtttttt aattgactct tcacttactg agaagataaa tgcgatagct ttaattccga
    16261 attccttgtc catcattttt tgatacattt caatcttctc cttttcctct aattccttta
    16321 aatccaacca ttccatatcc tgtatcaaac aacaaatctc tttacaagct aggaaagctt
    16381 gtttgctgca ttccaagacc tttctcctgt ataaaacaca agaatgtgca ccatgggtag
    16441 tacatataag aaaagaagag atttttttct cttcatatca attacttaat acttgtttta
    16501 aattttcaac agatatcgaa caaaccttag aaatttcatt gacactcatt ccatttttta
    16561 tcattgtctt gtacaaaaga acctgtccta gttctacacc ctgttcgatt ccttgttcaa
    16621 tgccttgctc gattcctttt cttatccctt cagcttccca ttcctttgac tttacttcaa
    16681 gccatttctt ttcagcctca cacatttcaa tctcctcctt ttcctctaat tcttctaaat
    16741 ccaaccattc tacatccgtt aatgtcttga ctacttttaa cacactgctc ttcacaagtc
    16801 caaagctatt gctctttcct tcataaatag actgatcata aatacttcga catatgtaga
    16861 atagattata cacgtcttct tcattaaaat tatatgaagt atttcctgtg atttcaatca
    16921 gattcatttt gtagtcatgg aaataggatt caaacctttc atccacctcg actagctggg
    16981 aaagcttatt tgcagcattc caaggctttt ctcctgtata aaatactaga atgtgcacca
    17041 tgggtagttt ttctttagcc ttattcttct ttaacttctt taacattctg tcatacgcta
    17101 catactcata tgtagccgct cttacaacca tcga
//
LOCUS       DBMDWL010000024        16549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1170_length_16549_cov_3.026204, whole genome shotgun sequence.
ACCESSION   DBMDWL010000024 DBMDWL010000000
VERSION     DBMDWL010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 16549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16549
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1170_length_16549_cov_3.026204"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(64..939)
                     /locus_tag="ACZPDN_02975"
     CDS             complement(64..939)
                     /locus_tag="ACZPDN_02975"
                     /inference="COORDINATES: protein motif:HMM:NF037639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIR1297617.1"
                     /translation="MKKIVLFIMMFLCSIMPIHANSLASITIELQDSIDDLSKENVDF
                     KLVQVAKLEDGYFVLNDAFQDLDVDFNVEFNAEQIENLCDEIQKKDVEGLHVKTDSKG
                     IAKVEDIEQGLYFIDPVDICGYENMRPMLVSVPQWQEDELVYSVVVYPKHSPFEKLIL
                     KKVDQETKQEILDEIEFTSYKDKDCKEKIASYKGSGTISILMRNQEMYLKETKAPKGY
                     DKSDRVLCVKVVDGSLYVDSKKLDSNVFEFENRKIHVPTGIEYHGNIYVTLGLVALVI
                     LLHLINKKLRRSCNG"
     gene            complement(936..1691)
                     /locus_tag="ACZPDN_02980"
     CDS             complement(936..1691)
                     /locus_tag="ACZPDN_02980"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HIR1297618.1"
                     /translation="MYVKRKGKNNLLFFLGFLLCVLPILLNLVFQSRMENVISSFYLE
                     EDTTQEKLVEDAYLNAELYNQQLFLNQERENYDTVLNFTSTQIMATIQIPVIDVDLAI
                     YHGTSDEVLNRGIGHFEFSSLPVGGKNSHCILTGHRGLPSAKLFTRLDELKKKDQIYI
                     HVCKKTLVYEVVDSFVVEPEDILDMTIEKDQDLLSLVTCTPYGINTKRLVVKAKRIFP
                     KKKNQPRKSYSMREFLFLIIPVVFLIVFKVVRR"
     gene            complement(1663..3234)
                     /locus_tag="ACZPDN_02985"
     CDS             complement(1663..3234)
                     /locus_tag="ACZPDN_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HIR1297619.1"
                     /translation="MKKRNLIYTGLLMLSLVTTPVYAQDKRVEVTNGICMLEEGSASI
                     VIQAHDKQPMNGKTFRMYKLFDAENSSDNASTSYTLNPVYAYKIKQIVSTKLNRSVDD
                     QDVLDYMRSLNNSNNQSEYRQFIEQVQNAIMNLDSYIIHVESTDVNGNIVIDKLAYGF
                     YLIDEVTNVINTHGASSLTMVNTASPKVVMQIKSDYPSVEKKVYEDDNSIGWNDMGDF
                     EINQDIPFKYVSKVPDMSGYKTYFFEFEDEMDPCLSLRADSVRLKIKDQFVVQNKYTY
                     TATDRGFKITFTDLKQIPNINKGDSIVLSYTAYLNESAVPKISKRGFENKVRVNFSND
                     PQNESKGQSPWDCVVCYTYQLNGLKSNEKNVKLKGATFKMYRDAAMRDQVKMKKTDSG
                     YVVTDGQSEDIVSNENGEFEIYGLDQGTYYLKETKAPAGYQLLQKPIELTISPKFVEN
                     RNNYRSNGDGLLKLEASSFNTPLNVSQSDAIIGLKVINKTGTKLPVTGSFGTILCVVT
                     GAGIMVYVCKKKREE"
     gene            complement(3231..5444)
                     /locus_tag="ACZPDN_02990"
     CDS             complement(3231..5444)
                     /locus_tag="ACZPDN_02990"
                     /inference="COORDINATES: protein motif:HMM:NF042849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIR1297620.1"
                     /translation="MKKKIYICMSILMVLFLSLTMVHANESTTYRLSEKEMEMMDEYS
                     KQNVNTPEFIQLRKEKAETYGLVDYCDENYILEEDFYDEYADAYLMQNGLMILDRYSA
                     GFESSSSFMMARSQSVTNVFHHRYTDRNGHYLANCLYQLSNSHYAFCAQGLNASPSSG
                     SVTSDPYLVSNANLKKCLYYGYNGPGDILTSRYGESAAIVLTDELVSNAYSSNCISKA
                     LSNGYHWDNVVSALWNEVTSKAEPSGYDVYFVDVAGQAYNWQGVMTSIQKLAYGVNSP
                     KGSVQIKKSSRLSDVSTNNVMYTLKDAKYGLYSDSSCTKKMGEFLLDEKGNSNVISNL
                     SVAKYYVREISAPKGYAKNDTVYPVDVKSNQVVEVAVSDVPYTNLVNLMLVKKDKDDQ
                     SKPLKDAQYEFKFYDQESSNKSPLRTWLMKTDADGKILMDDAHKISGDAFYKDVDGMV
                     CLPLGTITVQEVVPPKGYLLDPTIFVQKLEEKNVMSTHFNSFKYFNAVDKANSLKLVK
                     VQEGTDIPLSNVTFKHFVNGSFYIGQDTTNEKGEIEFVALSSGKHTLQEFKTIDGYVL
                     DASPIEFEVLENGSITGVNSVIKVENKVKPFSLKIQKKNDRNELLNGAVFGLFKDEKC
                     LECLEEKTTQDGVVVFDGLKNKETYYVKEVKAPAGYRMNKQVYCLNTDFVPVNNQFDV
                     YINQEKVNGLYENGTINMEFINQRMVKLPHTGSAKVLFMSVMGMFLMAKKLKEKE"
     gene            5636..5711
                     /locus_tag="ACZPDN_02995"
     tRNA            5636..5711
                     /locus_tag="ACZPDN_02995"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5668..5670,aa:Gln,seq:ctg)
     assembly_gap    5737..5746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5787..6893)
                     /gene="ftsZ"
                     /locus_tag="ACZPDN_03000"
     CDS             complement(5787..6893)
                     /gene="ftsZ"
                     /locus_tag="ACZPDN_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1297621.1"
                     /translation="MTEFNQVANIKVFGVGGGGSNAVNRMVADGVKGVDFYICNTDVQ
                     VMKNSPCDNKIVLGKETTKGLGAGGNPEYGRKAAEESEAEIRESVKGSDMVFITAGMG
                     GGTGTGAAPLIAKISKEEGALTVAVVTRPFTFEGRRRANNAKDGIEELKKYVDSLIIV
                     SNDNLLDVIGRKPIEEAFQAADNVLRQGVQTISDLIAVPALVNLDFADVRSVMQNQGR
                     ALIGIGMAEGEDKAISAAEKAIQSPLLEAQISGAKSAIVNITGGDKVSLFDAQNAVSV
                     IQDAAGGDVDCIFGIAINEQLGDAIIVTVIATGFEEPKEKQRKRRTEPVIKQVANDAL
                     FTEPKVQAPVNSGVVTSVENNSNDEQIPNFFFNR"
     gene            complement(6913..8172)
                     /locus_tag="ACZPDN_03005"
     CDS             complement(6913..8172)
                     /locus_tag="ACZPDN_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297622.1"
                     /translation="MSKKRVYAAIEIADQEIRLIVLEIFEARYNVLRTERVACSGVDK
                     NQKIVDESAIVTAIRQAITNAQAALGYRIERVLLAVPSVNVMRSSQKVRVQIEDGTKN
                     IRLFHIQQGYKNAIQKRLNEDVEFVNANKVTYEVNGTVSQKLPLNQECEDFMMDVDLL
                     YADKETIYSYARCVEQANLEILDLCLDSFAIGQETAALAQSTERVTIQIDLEQNHSTL
                     ALFSTGRLMTCTTLDFGYLWFIEDIQKKYKLSDEVCYRLLQNIFSGQEDENSDVIIYI
                     EQREDMRIEITANELAKACLPRIRQWIAAVNDACLPIVRQGKSRYVITGQGSNIPVLK
                     DLDKSFNASAMIYQEQAIGARDGAFVCGLGMAYAWQDINRIHHDDRISANNNELEASI
                     DSINQKASSVEGGFTKKLKNAILAEEE"
     gene            complement(8299..8847)
                     /locus_tag="ACZPDN_03010"
     CDS             complement(8299..8847)
                     /locus_tag="ACZPDN_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297623.1"
                     /translation="MSREVVVQRIEKSDLIDAKKELMIEIVQNKKEDDFDKKIDRLYR
                     QFSKSSSFKKHAEKMVCRDDEKLKICNMMLVMSVTLTLFFLKAVLFSSYVVNFSVDAI
                     VGCLAFVFSFINVRTKYRTLRRYNLVRDFIYLDGLSLLLCILFKMFFPVQVDFSLIIL
                     LVDYYLCKKRFKEKAFKMLEAN"
     gene            complement(8832..10154)
                     /locus_tag="ACZPDN_03015"
     CDS             complement(8832..10154)
                     /locus_tag="ACZPDN_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HIR1297624.1"
                     /translation="MNTIEISNELIQFIESSPSMFHSIKTIRTYLEEDGFTYLPESCA
                     WDVKEGGKYYTIRNHSSLIAFKVGKDLDSYHFQMCASHSDSPTYKVKNVPELEGPKEY
                     LRLDVEAYGGMIDNTWFDRPLSVAGRVLVRNGSKIESKLLYIDKDILMIPNVAIHFNR
                     EVNNGYKYNRQIDLCPMFSCGALKKGAFDKMVADELNVKAEDILGKDLFLVNRQKGLV
                     WGLENEFVSSPKLDDLQCAFVSLKAFMEAENEKSVNVYCCFDNEEVGSNTKQGAMSTF
                     LKDVLHRINNGLGKSEEEYYQAIAKTFLVSCDNAHALHPNHPEKTDAVNYVTMNGGIV
                     IKESANQKYTTDAFSRALFTGVCQNVNVPVQSFANRSDSVGGSTLGNLSNTQVSIHAV
                     DFGLAQLAMHSCFETAGVKDTEYAIIALKEFYETNVQIDEAESVCLEK"
     gene            complement(10213..11631)
                     /locus_tag="ACZPDN_03020"
     CDS             complement(10213..11631)
                     /locus_tag="ACZPDN_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1297625.1"
                     /translation="MNDSQKMKWYTLAFMCFSTLWGFGNVLNGFMYFNGIQVIFSWIL
                     MFALYFVPYALMVGELGSAFKNSGGGVSSWIHETTGPKAAYYAGWTYWACHITYIASK
                     GSGGLKALSWMIFQNSETYASFNTKAVQFATLAVLLLFSYVASRGLNPLKKMATIAGS
                     SMFVMSILYIVMMFAAPAINPNGGYLAMDFSFKNLIPTFDWKYFSSLSILVFAVGGCE
                     KISPYVNKVEDPSRGFPKSMIFAAIMVIVCAIFGTIAMGMMFDPAVIDANFDSYNANG
                     SYWAFQRLGEYYGMGNVLMIVYAACNCIGQFSTLVISIDAPLRMLLDNEDARQYIPTK
                     LLHKNDAGAYSNGIIMVDILAGAIILAQALVPNADTVLRQLTQLNSVTMPMRYLWVFF
                     AYVMLRKKTDVFHRDYKFVKNNTVAIVFGAWCFVLTAACCLLGMYKAGDMYTTFLNII
                     TPVVLTALGLILPAIKKKEAKN"
     gene            complement(11741..13897)
                     /locus_tag="ACZPDN_03025"
     CDS             complement(11741..13897)
                     /locus_tag="ACZPDN_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIR1297626.1"
                     /translation="MILRMAFIGTLVVANVLFTMVTHRHLWSQKDVLDKRIASSIVEK
                     TVTAKRGTIYDRNHTVIAQQSKAYTIVAYLDSGVVDANGNPNYVKNIESTAKKLKSVL
                     GDEVDEKTLIKIMRSAKKAGKSQTELGAGTKRIKKSVMKEIKKLKIDGIGFIDAVSRY
                     YPATPYSSNLVGFAAFDEDTQSIEGKMGLELSLNELLKGKNGSEQYQQTVDGSKLPGT
                     TKVIEQAKNGNDVVLTLDSSLQSTVESQLQATMENENAKSAWCIVMEVETGKVLAWAS
                     YPTFDQNEHKEIPSYQDAISNSTYEPGSVMKPFTYAIAMDTNVYPYNQTFSSYQFWYN
                     YDPNTAKISRVAIGTKTPYPYIADALDEDFGTITFDQGLAFSSNVGICELLANYVNYS
                     QYCDYLDKFGFFQKVNTPYVAQSLGVKNVGLPTDYLSTGFGQASSITVLQLCQAYTSI
                     FNDGTMMRPYVIDSIVDSDTKQTVKKYKKKAVGTPISSQSAKEVQELMSHVTDEGASG
                     HRFKMDGIDLLMKTGTAQIYNENLGKYDPAYHTSSVMAAAPANDPKVMVYYGLVSTNI
                     TSYSAEPFKKIMRDTLQTYGVSSTPTTQTQDTYEKWESYSMPSLVNHTIDYAHEKMKD
                     KKVHFEVIGDGTSVVGQYPDANITINSNDRIFVLTNGSKITMPNMTGWTRKDITVFWQ
                     LTGIGIKTSGYGKVTSQNVEEGTTISTDTKIEVTLE"
     gene            complement(13952..14227)
                     /locus_tag="ACZPDN_03030"
     CDS             complement(13952..14227)
                     /locus_tag="ACZPDN_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297627.1"
                     /translation="MQRKFNLTGALVLTLFVLSLVFYFASKTYVSSMNLSLSVQESEL
                     TSEIAQKEQTVEELQNEVNTLQEKGRLLGMLDNEVSDNENNIYVMND"
     gene            complement(14233..15159)
                     /gene="rsmH"
                     /locus_tag="ACZPDN_03035"
     CDS             complement(14233..15159)
                     /gene="rsmH"
                     /locus_tag="ACZPDN_03035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1297628.1"
                     /translation="MEHISVLLNESIDMLQIKEDGIYVDCTLGRGGHSSEILKRLTSG
                     HLYAFDCDQNAIDESMPRLKAIGDQFTLIHSPFENLKSELAKRGVNFVDGIFMDLGVS
                     SPQFDDAQRGFSYRWDARLDMRMDQSQELDAYVVVNTYDKEDLIRILKRYGEEPFAVK
                     IADKICKARETAPIETTFELVDVIKSALPAAVLRKKGHPAKQTFQAIRIEVNHELEQL
                     ETVLHDGLTMLNPHGRMAVITFHSLEDRIVKEIFKEVAVAKKVNKRLPQVGTENLEYQ
                     LVNRKPVLASSEELEMNNRAHSAKLRGIEKKG"
     gene            complement(15166..15597)
                     /gene="mraZ"
                     /locus_tag="ACZPDN_03040"
     CDS             complement(15166..15597)
                     /gene="mraZ"
                     /locus_tag="ACZPDN_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1297629.1"
                     /translation="MFMGEYAHNIDRKGRLIMPAKFREELGEHVVVNRGLDGCLYVYT
                     VEQWQQVYQKLSTLPSTNKDARMYQRMMLSKAAECEMDSQGRILIPSSLIALASLEKE
                     CLIIGVANHLEIWSKQRWEALEEEQSASFEEAAEHLSEIMG"
     gene            15845..>16549
                     /locus_tag="ACZPDN_03045"
     CDS             15845..>16549
                     /locus_tag="ACZPDN_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014683.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR1297630.1"
                     /translation="MSFLYFILGLCPILWLIFALTVLGWPTYKAAFGSLIISALLSIA
                     IWQQSFLNTISACGEGFLMALWPIIVVIIAAVFTYNLSLRTRGMEIIKQMITSVSSDK
                     RILVLLVAWCFGGFMEGMAGFGTAIAIPASMLVALGFEPLFSCLVCLIANGVPTPFGS
                     IGIPTVTLANLVGLENTQLAFTQTLQLAPFMLLCPFLIVMATGKGVKAFKGVTAVTLV
                     SGLSFLIPQMIVAKFVG"
BASE COUNT         5141 a         3638 c         2238 g         5522 t
ORIGIN      
        1 agaccgatat tcagattttt aaatcttgaa tattcggtcc ttttttatta agctgaaact
       61 tctttatccg ttacagctcc ttcttagttt tttattgatt aaatgtaaaa gaatcacaag
      121 tgcaactaat cctaaagtca catagatatt tccatgatat tcaatccctg ttggaacatg
      181 aatctttcta ttttcaaact caaatacatt cgaatccaat ttctttgaat ccacatacaa
      241 tgaaccatca accactttta cacaaagcac tcgatccgat ttatcatacc cctttggagc
      301 ctttgtctct tttaaataca tttcttgatt gcgcatcaaa atcgaaattg taccacttcc
      361 cttatacgaa gcaatctttt ctttacaatc cttgtctttg tagcttgtaa attcaatctc
      421 gtctaaaatt tcctgttttg tctcctgatc tacctttttt agaatcaact tttcaaacgg
      481 actatgcttc ggataaacta ccacactata aacaagttca tcctcttgcc attgcggaac
      541 actaactaac attggacgca tattttcata tccacaaata tctacaggat ctataaaata
      601 caagccttgc tcaatatctt ctactttggc aatcccttta gaatctgttt taacatgtaa
      661 accctccaca tctttctttt ggatctcatc acacaaattt tcaatttgtt cagcattgaa
      721 ttccacatta aaatccacat ctaaatcttg aaatgcatca ttcaacacaa aatatccatc
      781 ttcaagtttt gcgacttgca caagcttaaa atccacattt tccttcgata aatcatcaat
      841 cgaatcttgt agttcaattg tgattgaagc aagactattc gcatggattg gcataatact
      901 acataaaaac atcatgataa atagaactat ttttttcatc ttctcaccac cttaaataca
      961 atcaagaata caactggaat tattaggaac aaaaattccc gcatcgaata cgattttctt
     1021 ggctgattct tctttttagg aaatatacgc tttgccttca caaccaacct tttcgtattg
     1081 ataccatatg gcgtacatgt aactaaactt aaaagatctt gatccttttc aattgtcata
     1141 tccaaaatat cttctggttc taccacaaaa ctatccacaa cttcataaac caaagttttc
     1201 ttacaaacat gaatatagat ttgatccttt ttctttaatt catctaaacg cgtaaaaagc
     1261 ttcgcactag gcaaaccacg atgccccgtc aatatacaat gactattttt acctcctaca
     1321 ggtaaacttg agaattcaaa atgcccaatg ccacgattta atacttcatc actcgtacca
     1381 tgataaatgg caagatcaac atcaatgact ggaatctgga tggtggccat gatttgtgtg
     1441 gaagtaaaat tcaaaaccgt gtcataattt tctctttctt gattcaaaaa taattgttga
     1501 ttgtatagtt cagcatttaa atacgcatcc tctacaagct tttcttgcgt tgtatcctct
     1561 tcaagataaa aacttgaaat tacattttcc atgcggcttt gaaataccaa atttaaaagg
     1621 attggcaata cacaaagtaa aaagccaaga aagaataata aattattctt cccttttctt
     1681 tttacataca taaaccataa tgcctgcacc cgtaactaca cacaaaattg tgccaaaaga
     1741 tcctgtgaca ggaagttttg tacctgtttt attaatgact tttaaaccaa tgatcgcatc
     1801 cgactgactt acattcaatg gtgtattaaa acttgaagcc tctaatttca acaagccatc
     1861 tccatttgaa cgatagttat ttcgattttc aacaaactta ggcgaaatcg taagctcaat
     1921 tggcttttgc agcaattgat atccagccgg tgcttttgtt tcttttaaat aatacgtacc
     1981 ctgatctaaa ccatagattt caaattcacc attctcattt gagacaatat cttcactttg
     2041 accatccgta acaacatagc cactatccgt tttcttcatt ttcacttgat cacgcatagc
     2101 tgcatcacga tacattttaa aagtagctcc ttttaacttt acattcttct cattactctt
     2161 taacccattc aattgatatg tatagcacac aacacaatcc caaggcgatt gtcccttcga
     2221 ttcattttgt ggatcatttg agaaatttac acgtacctta ttttcaaatc ctcttttaga
     2281 aattttaggt accgcactct cattcaaata agccgtgtac gataaaacta tagaatctcc
     2341 tttattgatg ttaggaattt gttttaaatc tgtaaacgta attttaaagc cacgatccgt
     2401 tgcagtatat gtatacttat tttgaacaac gaattgatct ttgattttta atctcacact
     2461 atccgctctt aaagataaac atggatccat ttcatcctca aactcaaaga aatatgtttt
     2521 atatccactc atatctggca ctttcgatac atatttaaat ggaatatctt gattgatctc
     2581 aaaatccccc atatcattcc aaccaatcga attgtcatct tcatatacct ttttttcaac
     2641 acttggataa tccgacttga tctgcatgac gacttttgga cttgccgtat tcaccatcgt
     2701 caatgaactt gcaccatgtg tattgatgac attggtaact tcatcaatca aataaaatcc
     2761 atatgctaat ttatcaatca caatattccc attgacatct gtactttcta catgaatgat
     2821 atacgaatct aaattcataa ttgcattttg aacttgctcg ataaactgac gatactcact
     2881 ttgattgttt gaattattta atgagcgcat ataatcaagc acatcttgat catctacact
     2941 ccgattcaat ttagtactta caatttgttt gattttataa gcatacacag gatttaatgt
     3001 ataactagta gaggcattat cacttgaatt ttctgcatca aacaacttat acatacgaaa
     3061 agtttttcca ttcatcggct gcttatcatg agcctgaatg acaatacttg cacttccctc
     3121 ttcaagcata caaataccat ttgtgacttc cactctttta tcttgtgcat atacaggtgt
     3181 tgtcactaaa gacaacatca acaatcctgt ataaatcaaa tttctttttt tcattccttc
     3241 tccttcaatt tcttagccat caaaaacata cccatcacac tcataaataa aacttttgca
     3301 ctacctgtat gcggaagctt taccattctt tgattgataa actccatatt tattgttcca
     3361 ttttcatata aaccatttac tttttcttgg ttgatatata catcaaattg gttatttacg
     3421 ggcacaaaat ctgtatttaa acaatacact tgtttattca ttcgataacc tgcaggagcc
     3481 tttacttcct ttacataata ggtttcttta ttttttaaac catcaaatac caccacacca
     3541 tcttgcgttg tcttttcttc taaacactct agacatttct catctttaaa taagccaaat
     3601 acggcaccat ttaaaagttc atttcgatca ttcttctttt gaatctttaa tgagaaaggt
     3661 ttcactttat tttccacttt aattacagaa ttgacgcctg taatacttcc attttctaga
     3721 acctcaaatt caattggact tgcatccaac acatacccat cgattgtttt aaattcttgt
     3781 agcgtatgtt taccacttga aagagccaca aattcaattt cacccttttc atttgtggta
     3841 tcttgcccaa tatagaatga tccattcaca aaatgcttaa aggtcacatt cgacaacgga
     3901 atatctgtac cttcttgcac ctttactagt tttaaggagt tcgctttgtc cactgcatta
     3961 aaatatttaa aactattaaa atgtgtcgac attacattct tttcttcaag cttttgaaca
     4021 aagattgttg gatctaataa atagccttta ggaggcacaa cctcttgtac tgtgatcgtt
     4081 cctagtggta aacaaaccat tccatccaca tctttataga aggcatctcc actaatttta
     4141 tgtgcatcat ccattaagat ctttccatcg gcatctgttt tcattagcca cgtacgcaac
     4201 ggtgacttat tacttgattc ttgatcataa aacttgaatt catattgcgc atcttttaaa
     4261 ggtttagatt gatcatcctt atcttttttg actaacatca aattcacaag atttgtataa
     4321 ggaacatccg atacagccac ttctacaact tgattgctct ttacgtccac aggatatacc
     4381 gtatcatttt tggcatatcc ctttggtgca ctgatttcac gaacataata tttcgcaaca
     4441 cttaaattcg aaatgacatt ggaatttcct ttttcatcaa gaagaaattc acccatcttc
     4501 ttagtacaac tagaatcact gtataaacca tactttgcat cttttaatgt atacattaca
     4561 ttgtttgtgc ttacatcact taatcgactg ctctttttaa tttgaacact tcccttagga
     4621 ctatttacac catatgccaa tttttgaatg cttgtcataa caccttgcca gttataagct
     4681 tgtcctgcaa catcgacaaa atacacatca taaccacttg gttccgcttt tgaagtgact
     4741 tcattccata aggcactgac cacattatcc cagtgatatc cattggaaag tgccttactg
     4801 atacaatttg acgaataagc attcgaaacc aattcatccg ttaatacaat ggcagcactt
     4861 tcaccataac gactcgtcaa tatatcacct ggcccgttat agccataata taaacatttt
     4921 ttcaaattcg cattacttac taaataagga tcggatgtaa cacttccact cgatgggctg
     4981 gcattcaacc cttgtgcaca aaaggcatag tgtgaattcg ataattgata tagacaattg
     5041 gcaagataat gtccattacg atctgtatag cgatgatgga aaacatttgt gacactttga
     5101 gatctagcca tcataaaact agaactcgat tcaaatccag cactatatcg atcgagtatc
     5161 atcaatccat tttgcatcaa atatgcatcg gcatactcat catagaaatc ctcttctaaa
     5221 atatagtttt catcacaata gtcgactaat ccataggttt ctgccttttc ttttcgcaac
     5281 tgaataaatt caggcgtatt tacattttgc ttactatatt catccatcat ctccatttct
     5341 ttttcactca aacgatatgt tgttgattca ttggcatgca ccattgtcaa agaaagaaac
     5401 aacaccataa gaatactcat acatatataa atcttctttt tcatttcttc acccccttgt
     5461 ttatatactc ttttcttctc acctataatt gtgacaactc aatgtgaaaa gaacgtgaat
     5521 ttccatcaat ttcaaacgat tgcacctatc tgatctaaaa cgcaaaaaaa caagtgcttt
     5581 acatgaaaaa acacttgtaa aaaaataacc aatagtgtat tataaatagg cacattgggg
     5641 tatagccaag cggtaaggca tcagactctg aatctgacat ttccttggtt cgaatccaag
     5701 taccccagcc atgcctagta cagagaagag attctcnnnn nnnnnnaata ctaaaaaaga
     5761 gagcctaggc tctctaaatg tgtgaattaa cgattgaaga agaaattagg aatttgttca
     5821 tcattcgagt tgttttcaac acttgttaca acacctgaat ttacaggagc ttgaactttt
     5881 ggttctgtaa ataatgcatc attcgcaact tgtttgatga ctggttcagt acgtctttta
     5941 cgttgttttt cttttggttc ttcaaatcct gtcgcaataa cagtaacgat aatcgcatca
     6001 cctaattgtt cattgattgc gataccgaag atgcagtcaa catctccacc agccgcatcc
     6061 tggattacag atacagcatt ttgagcatca aataagctaa ctttgtcacc accagtgata
     6121 tttacgatag ctgatttagc accagaaatt tgtgcttcca ataatggaga ttggattgcc
     6181 ttttcagcag cagaaattgc tttgtcttct ccttctgcca taccaatacc gatcaatgca
     6241 cgtccttggt tttgcataac gctacgaaca tccgcaaagt ctaagttaac caatgcagga
     6301 accgcaatca aatcagagat tgtttgaaca ccttgacgta atacattatc tgcagcttgg
     6361 aatgcttctt cgattggttt acgtccaatc acatctaata agttgtcatt tgatacaatg
     6421 atcaatgagt caacatattt tttcaattct tcaataccat ctttggcatt gtttgcacga
     6481 cgacgacctt caaaagtgaa tggacgagtc acaactgcaa ctgttaaagc accttcttct
     6541 ttagaaatct ttgcgatcaa tggagcagca ccagtacctg ttccaccacc cataccagca
     6601 gtgataaata ccatgtcgct tccttttact gattcgcgaa tttcagcttc actttcttca
     6661 gctgctttac gtccatattc tggattacca ccagcaccta gaccttttgt agtttctttt
     6721 cctaaaacga ttttattatc gcaaggagaa tttttcatta cttgtacatc tgtgttgcag
     6781 atatagaaat caacaccttt aacaccatca gcaaccatgc ggttaacggc attggatcca
     6841 ccaccaccaa caccaaatac tttaatattt gctacttgat taaattcagt catataatat
     6901 tttcctctct ttttattctt cttccgctaa aatagcattc tttaatttct ttgtaaatcc
     6961 accttcaaca cttgatgcct tttgattgat tgagtcaata cttgcttcca attcattatt
     7021 gttagcactg attcgatcat catggtgaat acgattgata tcttgccaag catatgccat
     7081 acctaagcca catacaaacg caccatctct tgcaccaatt gcttgttctt gatagatcat
     7141 tgcactcgca ttgaaagatt tatctaaatc cttcaacaca ggaatattac ttccttgtcc
     7201 ggtaatgaca tatctagatt ttccttggcg aacaattggt aaacatgcat cattgacagc
     7261 tgcaatccat tgacgaatac gaggtaaaca agctttcgca agttcatttg ccgtaatctc
     7321 aatacgcata tcttctcttt gttcaatata aatgattaca tcactatttt catcttcttg
     7381 acctgagaaa atattttgca acaatctgta acatacttca tcactcaact tatatttctt
     7441 ttgaatatct tcaataaacc ataaatatcc aaaatccaat gttgtacaag tcatcaaacg
     7501 acctgtgcta aacaaagcta aagtagaatg attctgttcc aaatcaattt gaattgtaac
     7561 tctttctgta ctctgtgcta aagcagctgt ttcctgacca atcgcaaatg aatctaaaca
     7621 tagatctaag atttcaagat ttgcctgctc aacacaacga gcatatgaat aaatcgtttc
     7681 cttatccgca tataataaat ctacatccat catgaaatct tcacattcct gattcaatgg
     7741 aagcttttga cttactgtac catttacttc ataagtaacc ttattcgcat ttacaaattc
     7801 tacatcttca ttcaatcgtt tttgaatcgc gtttttatat ccttgctgaa tatggaataa
     7861 acgaatgttc ttcgttccat cttcaatttg tacacgtacc ttttgtgagc tacgcattac
     7921 atttacacta ggaacagcca ataatacacg ttcaattcgg tatcctaaag ctgcctgtgc
     7981 attcgttata gcttgacgaa tggctgtaac aatagcactt tcatcgacaa tcttttgatt
     8041 tttatctaca cctgaacaag caactctttc tgttcttaaa acgttgtaac gagcttcaaa
     8101 tatctctaaa acgataagac gaatctcttg atcagcaatt tcgatagccg cataaactct
     8161 tttcttactc attcgacgtc tcctttttac atactactta acaataatac cataattgaa
     8221 ttaaaaatag aaggctagtc tttaaaatca aatgagaaaa aaacaggagt atccaagcct
     8281 aggatacccc ctatattgtt aatttgcctc caacatttta aaagcttttt ccttaaatct
     8341 tttcttacac aaataataat ccaccaataa aataatcaat gaaaaatcca cctgcaccgg
     8401 aaagaacatt ttaaacaaaa tacaaagaag taaagataaa ccatccaaat agataaagtc
     8461 acgaactaga ttatatcttc ttaacgtacg atactttgta cgaacattga taaatgagaa
     8521 tacgaatgct agacaaccaa caatcgcatc cacactaaaa tttaccacat aactcgaaaa
     8581 caagacagcc tttaaaaaga acaaagtcaa agtcacactc atcacaagca tcatgttaca
     8641 aatctttaac ttttcatcat cccgacaaac catcttttcc gcatgttttt taaaagaaga
     8701 actcttagaa aattgacgat acaaacgatc gatcttctta tcaaaatcat cctctttttt
     8761 attttggaca atttcaatca tcaattcctt tttcgcatcg atcaaatccg acttctctat
     8821 tctttgaaca actacttctc tagacatacc gattcagctt catcgatctg cacatttgtt
     8881 tcatagaatt ccttcaatgc gataatcgca tattccgtat ccttaacacc ggctgtttca
     8941 aaacatgagt gcatcgcaag ctgcgctaaa ccaaaatcaa ccgcatgaat acttacctgc
     9001 gtatttgata agttaccaag tgttgaacct ccaacactat cactacggtt cgcaaaactt
     9061 tgtactggca catttacatt ttgacaaacc cctgtaaata acgcacgact aaacgcatcc
     9121 gttgtatatt tttggtttgc actttcctta atgacaatac caccattcat tgtgacatag
     9181 ttaacggcat ctgtcttttc aggatgattt ggatgaagtg catgtgcatt gtcacaactt
     9241 actaagaaag tttttgcgat agcctgataa tactcttctt cactcttacc taaaccatta
     9301 ttgatacggt gcaatacatc ttttaagaaa gtagacatag ctccttgttt tgtatttgag
     9361 cccacttctt cattgtcgaa gcaacaatat acattcacac tcttctcatt ttcagcctcc
     9421 ataaaagcct tcaatgaaac gaatgcacat tgtaagtcat ccaatttagg acttgaaaca
     9481 aattcatttt ccaatcccca aactaaacct ttttgacgat tgactaagaa taagtcttta
     9541 cctaaaatat cctctgcctt aacatttaat tcatccgcaa ccatcttgtc aaaagcaccc
     9601 tttttaagtg caccacaaga gaacattgga cataaatcga tttgacgatt atatttataa
     9661 ccattattca cttcacgatt aaagtgaata gccacatttg gaatcatcaa tatatcttta
     9721 tcgatatata ataatttaga ttcaatcttc gaaccattac gaacaagcac acgacctgca
     9781 acacttaaag gacgatcaaa ccaagtatta tcgatcatac caccataagc ctctacatct
     9841 agacgcaaat attcttttgg tccttcaagt tctggcacat tctttacttt ataagttgga
     9901 gaatccgagt gacttgcaca catttggaaa tgataagaat ctagatcctt acccacctta
     9961 aacgcaatca aacttgagtg gttacgaatt gtataatatt ttccaccctc tttaacatcc
    10021 caagcacaag attcaggaag atatgtaaat ccatcttctt ctaaataagt acgaatcgtt
    10081 ttgattgaat gaaacatact tggacttgat tcaataaatt gaattaattc atttgaaatt
    10141 tctattgtat tcattataaa aacatctcct caactatata taattatcca caaatatgaa
    10201 gaaaaggcaa aattaatttt ttgcctcttt tttcttgatt gctggtaaaa tcaaacctaa
    10261 agctgttaat acaactggag tgataatatt taagaatgtt gtatacatgt ctcctgcttt
    10321 atacatacca agtaagcagc aagcagccgt taatacaaag caccatgcac caaatacaat
    10381 cgcaactgtg ttattcttca caaatttata gtcgcggtgg aaaacatctg ttttcttacg
    10441 caacataaca taagcgaaga atacccataa ataacgcatt ggcattgtta ctgagtttaa
    10501 ctgcgtcaac tgacgaagta ctgtatctgc atttggaact aaagcctgag ccaagatgat
    10561 tgcacctgca agaatatcaa ccatgatgat accatttgaa taagcacctg catcattttt
    10621 gtgtaacaac ttagttggaa tatattgacg agcatcttcg ttatccaata acattctcag
    10681 tggagcatca atactaatga ctaatgtaga gaattgtcca atacagttac aagctgcata
    10741 cacaatcatc aacacattac ccataccata gtattcacct aatctttgga atgcccaata
    10801 agaaccattc gcattgtatg agtcaaagtt cgcatcgatt acagctggat caaacatcat
    10861 acccattgcg attgtaccaa aaatagcaca aacgataacc atgatagccg caaaaatcat
    10921 actcttagga aatcctcttg aaggatcttc aactttattt acataaggtg aaatcttttc
    10981 acatccacca accgcaaaga ctaagattga caatgaactg aaatatttcc agtcaaatgt
    11041 tggaattaag tttttgaaac tgaaatccat agctaaatat ccaccatttg gattgattgc
    11101 tggtgccgca aacatcatta cgatataaag aatactcata acgaacatac tagaaccagc
    11161 gattgttgcc attttcttca atggatttaa accacgactt gctacataac tgaatagtaa
    11221 taatacagcc aacgtcgcaa actgaaccgc ttttgtgtta aaagatgcat aagtttctga
    11281 gttttggaag atcatccagc ttaatgcttt taaaccacca gaaccctttg aagcaatgta
    11341 cgtaatgtga catgcccaat aagtccaacc tgcatagtat gcagccttag gacctgttgt
    11401 ttcgtgaatc caagaactta cacctccacc tgaatttttg aatgctgaac ctaactcacc
    11461 taccattaaa gcataaggta cgaagtaaag cgcaaacatc aaaatccaag aaaagataac
    11521 ttgaattccg ttgaagtaca taaatccgtt tagtacgttt ccaaatcccc aaagtgtaga
    11581 aaagcacata aacgcaagtg tgtaccactt cattttttgt gaatcattca ttttactctc
    11641 tcctctttca ctgtatatat aattttcata tataaaaatg gttttgtcaa ctctcattta
    11701 tatgacgcat caaaaaaagc gcctataata gacgcttcac ttattctaaa gttacctcaa
    11761 tctttgtatc cgtggaaatc gttgtaccct cttcaacatt ttgacttgtt actttaccat
    11821 aaccactcgt ttttatacca atccccgtca actgccagaa caccgtaata tccttacgcg
    11881 tccaacccgt catatttggc attgtgatct tactgccatt tgtcaacaca aagatgcgat
    11941 catttgaatt gattgtgata ttcgcatctg gatattgacc cactacactt gtaccatccc
    12001 caattacttc aaaatgtact tttttatctt tcatcttctc atgcgcataa tcaattgtat
    12061 gattcactaa acttggcatc gaataactct cccacttctc atacgtatct tgtgtttgtg
    12121 ttgtaggtgt tgaagaaaca ccatatgtct gcagcgtatc acgcatgatc tttttaaatg
    12181 gctctgccga atacgaagta atatttgtgg aaaccaagcc ataatatacc atgacctttg
    12241 gatcatttgc aggcgctgct gccatgacac ttgaagtatg ataagccgga tcatatttac
    12301 ctaagttttc attatagatc tgtgccgttc ccgttttcat caatagatcg ataccatcca
    12361 ttttaaaacg atgaccactc gctccttcat ccgttacatg cgacatcaat tcctgcactt
    12421 cttttgcgct ttgagatgat atcggtgtac ccacagcctt tttcttatac ttctttacag
    12481 tctgttttgt atcactatct acaattgaat caatgacata aggacgcatc attgttccat
    12541 cattaaaaat ggaagtataa gcctgacaaa gctgcaaaac tgtaattgaa cttgcctgac
    12601 caaatccagt cgacaaataa tcggttggaa gtcccacatt ctttactcct aaagactgtg
    12661 ctacataagg cgtatttacc ttttgaaaga atccaaactt atctaaataa tcacaatact
    12721 ggctataatt gacataattt gccaacaact cacaaatccc cacattactt gagaaagcca
    12781 agccttgatc aaatgtgatc gtaccaaaat cttcatctaa ggcatcggca atatatggat
    12841 atggcgtctt agtaccaatc gcaacacgag agatctttgc ggtatttgga tcatagttat
    12901 accaaaattg atacgatgaa aaagtttgat tataaggata cacatttgta tccatcgcaa
    12961 tcgcatatgt aaatggcttc attaccgaac caggctcata tgtactatta ctaatcgcat
    13021 cttgatagct aggaatttct ttatgttcat tctgatcaaa tgtaggatag cttgcccaag
    13081 ccaaaacttt acctgtttcc acttccatca caatacacca tgctgatttc gcattttcat
    13141 tttccatcgt agcctgcaat tgactttcaa ccgtcgactg caaacttgaa tctaatgtta
    13201 aaacaacatc atttccattt ttagcttgtt caatcacttt cgtcgtaccc ggaagcttag
    13261 aaccatccac tgtctgttga tattgttccg aaccattttt ccccttcaac aattcattta
    13321 acgataattc aagtcccatt ttgccttcaa tagattgcgt atcttcatca aaagctgcaa
    13381 atccaacaag attggatgaa taaggagtag caggataata acgagatacc gcatcaataa
    13441 agccaatacc atcaatcttt aattttttga tttccttcat gacacttttt tttatacgct
    13501 tcgtacccgc acccaattcc gtttgcgatt ttcccgcctt ttttgccgac ctcataattt
    13561 taataagcgt cttctcatcc acttcatcac ctaatacact cttcaatttt ttggccgtag
    13621 actcaatatt ctttacataa ttcggattcc cattggcatc tacaactccc gaatctaaat
    13681 aggcaacgat tgtgtaggct ttactctgtt gtgcaatgac agtatgatta cgatcataaa
    13741 tcgttcctct ttttgccgta actgtctttt ccacaatact tgatgcgata cgtttatcca
    13801 atacatcttt ttgcgaccat aaatgcctat gtgtgaccat agtgaaaaga acattagcta
    13861 ccactaatgt tccaataaat gccatccgta aaatcatttt aaagagttca ttattacttt
    13921 ttcgaaccga cattatacat caccactcca tctagtcatt cattacatat atattatttt
    13981 cattatcaga gacttcattg tcaagcattc ctaacaatct gcccttttcc tgtaacgtat
    14041 tgacttcgtt ttggagctct tctacagttt gttccttttg agcaatctca ctagtcaatt
    14101 cactttcttg aactgaaagc gaaagattca tcgaacttac atatgttttt gatgcgaagt
    14161 agaagaccaa tgacaacaca aataaagtta aaacaagtgc acctgttaaa ttaaactttc
    14221 tttgcatatg catcatcctt tcttctctat ccctcttagt tttgcgctat gcgccctatt
    14281 attcatttct aattcttcac tacttgctaa aaccggtttt cgatttacca gctgatattc
    14341 taaattttct gtccccactt gtggcagcct cttattgacc tttttggcaa cggccacctc
    14401 tttaaaaatc tcttttacaa ttcgatcctc taaagaatgg aatgtaataa ccgccattct
    14461 tccatgtgga ttcaacatcg ttaacccatc atgcaatacc gtctctaatt gttctaattc
    14521 atgatttact tcaatacgaa ttgcctgaaa cgtttgtttt gctggatgcc ccttctttct
    14581 taaaacagcc gctggaagcg ccgatttaat cacatccacc aattcaaatg tcgtttcgat
    14641 tggtgcagtt tctctagcct tacaaatctt gtcagctatt tttaccgcaa aaggctcttc
    14701 gccataacgc tttaaaatac gaatcaaatc ttctttatca tacgtattca ctacaacata
    14761 ggcatctaat tcttgtgatt gatccatacg catatctaag cgtgcatccc aacgatagct
    14821 aaatcccctt tgtgcatcat cgaattgcgg gcttgataca cccaaatcca taaatatacc
    14881 atccacaaaa tttacaccac gcttcgctaa ttcgctcttt aaattttcaa aaggagaatg
    14941 aatcaaagta aattgatctc caattgcctt taaacgcggc atgctttcat caatcgcatt
    15001 ttgatcacaa tcaaacgcat ataaatgtcc acttgttaaa cgtttcaaga tttcactact
    15061 atgtccccct cgacctagtg tgcaatctac atagatccca tcttctttta tttgtagcat
    15121 gtcaatcgat tcattcaata atacactaat atgttccatt cattactacc ccataatttc
    15181 tgataagtgt tcggccgctt cttcaaaaga tgcactctgc tcttcttcta aagcttccca
    15241 gcgttgttta gaccaaattt ccaaatggtt cgcaacacct ataatcaaac attctttttc
    15301 aagagaagcc aaggcgatta aagaggatgg aattaaaatt cgaccctgac tatccatttc
    15361 acattctgct gcttttgaca acatcatacg ctgatacata cgtgcgtctt tatttgtcga
    15421 aggcaaagtt gatagctttt gatacacttg ctgccactgc tctaccgtat atacatacag
    15481 gcatccatca agtcctcggt ttacgacaac atgttcacct agttcctcac gaaactttgc
    15541 tggcataatc aatcgcccct tacgatctat gttatgcgcg tattccccca tgaacatgtg
    15601 tcttcacccc cactctatga cacaatatgc tactttgtac cactataata catcattcta
    15661 tctaaaaacg ccacaagatt atcaaaaatt gtactaattt ttttatttca tagttagtca
    15721 aataataacc tcatatttaa ccctcatttg ttgtttgatt gcgcttacac gcctattttt
    15781 agcgattttc ttttgacaaa catatacact ctgatataat tcgaacgtac aagggagaga
    15841 tgatatgagt tttctatatt ttattttggg actttgtccc attttgtggc ttatatttgc
    15901 gctaaccgta cttggatggc ccacatataa agctgccttt ggttctttaa ttatttctgc
    15961 actattatct atagcgattt ggcaacaaag ttttctaaat acgattagtg cttgcgggga
    16021 aggtttctta atggctttat ggcctattat tgttgtgatt atcgcagctg tatttacata
    16081 taatttgtca ttacgtacac gcggcatgga aatcattaaa caaatgatta ccagcgtttc
    16141 aagtgataaa cgaatcttag tcttattagt tgcatggtgt tttggaggat ttatggaagg
    16201 tatggccggt tttggaactg ccattgccat tcctgcaagt atgcttgtag ctttaggttt
    16261 tgaaccttta ttttcatgct tagtttgctt gattgccaat ggtgttccta ctccatttgg
    16321 atctattggt attccaaccg ttactttggc aaatttagtt ggacttgaaa atacccaatt
    16381 ggcatttacc cagacacttc aattagctcc atttatgttg ctatgtccat tcttaattgt
    16441 catggcaaca ggaaaaggtg ttaaggcatt caaaggtgtt acggccgtaa ctttggtatc
    16501 aggacttagt ttcttgattc cacaaatgat tgtcgctaaa tttgtagga
//
LOCUS       DBMDWL010000025        16366 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1189_length_16366_cov_2.901445, whole genome shotgun sequence.
ACCESSION   DBMDWL010000025 DBMDWL010000000
VERSION     DBMDWL010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 16366)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16366)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16366
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_16366_cov_2.901445"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(69..464)
                     /locus_tag="ACZPDN_03050"
     CDS             complement(69..464)
                     /locus_tag="ACZPDN_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024094.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1297631.1"
                     /translation="MKKIISLFMALGMVLSLAACSTNQSPSESTPEEQPPTDEAGKTL
                     VVYYSATGNTENVANYIATATDGDLFELEPAEPYSDADLNWTDDNSRVVREHDNPDER
                     DIALVKSTVENWDEYDTIFIGYPKMEYSL"
     gene            complement(486..1655)
                     /locus_tag="ACZPDN_03055"
     CDS             complement(486..1655)
                     /locus_tag="ACZPDN_03055"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1297632.1"
                     /translation="MNNFIFENSTKVFFGKGCVKEYLCCLTKGYGDTVMLCYGGGSIK
                     KNGIYDEVTNILEAAGKTVVEFSGIMANPTYAKVLEGAKLAKKNDVSLLLGIGGGSVM
                     DCCKAISIAARYDGDVWEDFWKRPGVFDFEPLPLGVIVTVAGTGSECNGGAVITNEEL
                     KIKTGRDYPQCNPKFALLDPTYTYSVPKKQMVSGSFDILSHIMEIYFSEPNEDNVSDD
                     ISEALMRSVIHNLRAAIQNPQDYSARSNLMWAATMAENRVIKLGKRMDFECHQMEHQL
                     GAYTNCNHGEGLAVLHPVYYRHIYKYGLPKFKRFAIEVMGVSAEAKTDEEIALAGIEA
                     LEDFIVEIGLPVSLQDLGVNENTDLKEIADSCALMPGSYKKMTHEEILAIFKECY"
     gene            complement(1697..2716)
                     /locus_tag="ACZPDN_03060"
     CDS             complement(1697..2716)
                     /locus_tag="ACZPDN_03060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1297633.1"
                     /translation="MTILRANVFVAALICIAVFLAGCSANMNSTESDTDFKGVETIVQ
                     TNQNDTEQYTQDTKISDVINDTVFGDYGRLIFPVNSSYYSGDTLGELGLTWYNNIAPD
                     KTVEIANYMKSHAENGDVIFYDIYTDEEKATDPAKEDTGLFFFKGNPGERFAVCNAGG
                     GFAYVGAMHDSFPHALELSKMGYNAFALIYRPGAQTACEDLARAISFIFENADELEVN
                     TDCYSLWGGSAGGRMAAWLGSYGTAAFGKKDLPQAWAVIMQYTGHNEYSESDPPTYAC
                     VGENDGIANWRTMEARLNRLNELGIPTEFHHYEGLRHGFGLGTGTVAEGWINDAVNFW
                     ENQMQ"
     gene            complement(2809..3711)
                     /locus_tag="ACZPDN_03065"
     CDS             complement(2809..3711)
                     /locus_tag="ACZPDN_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1297634.1"
                     /translation="MYNPQLETFIRVADAGSFNKAAEESFITPTAVIKQINLLEDSLG
                     VKLFDRTHRGLHLTKAGHSLYQDSKYIIQYCRDSITRAKNAMQEDSNIIRIGSSPITP
                     AQLLMELWSRVQALHPNIKFQIVPFENTPENAREILANLGKNIDIVGGIFDDTMLEVR
                     ACAGLELNRGPFHCAVSIHHRLATKDKLQLSDLYGENLLLMHRGWSRYVDQLRDDLWQ
                     NHPQINIVDFDFYGMDIFNRCENTNDVLLAIPGWANVHPLLKVIPVEWNHCIPYGILH
                     SPTPTPIVQQFLDAAEKVSKELYH"
     gene            complement(3977..4105)
                     /locus_tag="ACZPDN_03070"
     CDS             complement(3977..4105)
                     /locus_tag="ACZPDN_03070"
                     /inference="COORDINATES: protein motif:HMM:NF025561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIR1297635.1"
                     /translation="MAKSEGVNEQLKVSNAMLWVQKMNNIRNKASEIINAEVIFPD"
     gene            4099..4530
                     /locus_tag="ACZPDN_03075"
     CDS             4099..4530
                     /locus_tag="ACZPDN_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297636.1"
                     /translation="MPFDLRAKKHFFSLIVYKGSDYIVRGQTEDGNNRLISGTSIGSI
                     QYQPNQDANGNYYNIGQYDENDEQDKRYDGQDWYYNEGTVDNPKWGVYYKHKYTETDE
                     GIFPLKVWYKQDVGYSDQKYNTINRSCFFMSYYEYFRLVLD"
     gene            complement(4713..5426)
                     /locus_tag="ACZPDN_03080"
     CDS             complement(4713..5426)
                     /locus_tag="ACZPDN_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1297637.1"
                     /translation="MKKIDAHAHLGYIGGWANVKMDADELISLMDTYEIETTMICVLD
                     NEEAYKAMQKYPGRIEGCVYVNPLEPNCLDLIDKYVKLGFKAIKLQPLRHAYCADSEI
                     VDPVLDKAEEYGIPVCIHSGHPPYSLPWQIGLLAERHPNCKVLMIHMGHGHGVYIDAA
                     LKMARRYPNIYLEMSGMPMHTKIKEAYDTVGHDRIMFGTDTPFHHPTVEMQKVLMCGV
                     DEQGLEDIFYNNAKKFFDV"
     gene            complement(5423..5938)
                     /locus_tag="ACZPDN_03085"
     CDS             complement(5423..5938)
                     /locus_tag="ACZPDN_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1297638.1"
                     /translation="MTNETTRRIAKTALMVALIFVCTFTIKLPNPATGGYTHMGDCMA
                     FLAVLYLGKKDGALAAGLGAALSDLLTGAMIWVLPTFIFKFIMGYIMGVILEKGNNEK
                     MDWIVGASLGGLFQCFAYTLFKIPLFTAAVAFASVPRLLAQTIMGLVIYAIIANVLAK
                     SKVLSLQGATA"
     gene            6071..6724
                     /locus_tag="ACZPDN_03090"
     CDS             6071..6724
                     /locus_tag="ACZPDN_03090"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1297639.1"
                     /translation="MKKLLMSFLLIFCIGCTFTFLNETNINISAKSTKETIMIDAGHG
                     GYDVGAESYYGDYEKDINLDIALLVGKQLKSYGYNVVYTHTSDSVSWPSNNKKDLQAR
                     CDLAKKKNADFFVSIHLNSSEYEANGYEIYCDFNNKNTLKVSNSILKQLDQFDYSTNR
                     GLLDTNETPLYVVKNNKVDAILIEAGFISDDSDLYYLKNHTKNIATAIAAGIKKSLN"
     gene            complement(6733..8496)
                     /locus_tag="ACZPDN_03095"
     CDS             complement(6733..8496)
                     /locus_tag="ACZPDN_03095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297640.1"
                     /translation="MIMKETKKMKFSECLSHLFVFVKPYRFKLLFGILMVITAQVTFA
                     LNPTVEGMITTQLAHDISSHQPIQIDKVVHILCILFTIYIVKTISQFLMAIFMTDAIQ
                     KTMFDVRNAIEDKIHHLPVSYFDQEKTGELLSRITNDVDTLSNALQQTLSRVISAVCT
                     FTFVLFMMFRINVLMTCIILVALPVIALLSKFVVKKSQPLFDDQQNTLADLNGTINEL
                     YDGYNEILSYNQQEHALKRFEKDNERMRVSSFKAQFVSSLINPLCSLITYLSIGCASL
                     VGCLQVLNGTIALGQLQAFIRYIWQINDPISQISQLSSAVQSAFSAMSRLFTFLNQPI
                     EEDVVSKCQIDEVRTIEFDHVQFGYDDNLLMKDVNIDVHQGQTIAIVGPTGAGKTTLT
                     NLLLRFYDVKGGSIKINGIDIRELSYHDLRKLFALVLQDTWLFEGSIEQNIAYGNEKA
                     LKNEIVEAAKQANVHHFIRTLTDGYDTLINEEGSNVSQGEKQLLTIARALLKNPEVLI
                     LDEATSSVDTRLERRLQGAMDRVMENRTCFVIAHRLSTIINADKILVLEHGDIVEQGS
                     HEELLNMNGVYARLYNAQFAK"
     gene            complement(8477..10216)
                     /locus_tag="ACZPDN_03100"
     CDS             complement(8477..10216)
                     /locus_tag="ACZPDN_03100"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297641.1"
                     /translation="MKLIWKYLMKYKKWFLLDFISVFGFALVELGIPTIISDMIDHGI
                     ETHDTAYLYSRFGLMAFISVIGVSGVVLLGYCCSKISTNITRDIRNDVFKHSQSFSAA
                     EMEKFGVSSMITRTNNDAYQIMLFLNVILKSALLTPVMMCVSVMLILQISLQLSYVVL
                     ATIPVVILGVIIVAKVSEPLSENQQKSIDHINQILRENITGIRVIRSFNKEDYEKKRF
                     SNENDFYRDQSAKLFKLMSCTEPSFFFLMNIATVIVYYLSCTLLNTNAITLGNVVAFV
                     EYLFHVMMSVLIFCMVFMMYPRANVSAKRIMEVLDTKPSIVNDKDCILLNSIETLSFK
                     DVSFSYPNGEEAVLKNIDFSCHKGQKIAVIGSTGSGKSTLVKLMNRFYDVSRGSIKIN
                     GVDIRKYDLESLHAQIGYVAQKAHMFKGSIQSNICFGKPEASDEEMVHAATVAQASDF
                     ISKRENGYLDEIAEDGTNISGGQKQRLSIARVILKKPSLYIYDDSFSALDFKTDATLR
                     KALKPEVKDAIFFVVAQRISTIMDSDMIIVLDEGQIIGLGTHLELLKNCSVYQEIAHS
                     QLTQKELDDYERN"
     gene            complement(10266..11969)
                     /gene="ptsP"
                     /locus_tag="ACZPDN_03105"
     CDS             complement(10266..11969)
                     /gene="ptsP"
                     /locus_tag="ACZPDN_03105"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1297642.1"
                     /translation="MLKGIAASAGVSVAKVYKLETPKVVIEKKAGVAEEEVKKFEDAL
                     EQTKKDIEGVKERAAKRLSDEELAVFDAHLMMAGDPELAGQIKAMIENDGVNAEYATE
                     QVANQMVEMFESMDNDYFRERAADIKDVTFRLKCNLLGLTIPDLTSIDEDSIIVAHDL
                     TPSDTAQLNEFVKGFATEIGGKTSHSAIMANSLEIPAVVGCPGVCDACKTGDTLALDA
                     LDGVVEINPDEETIAKYEAKAAAYLKEKEELKVLKNEKSVTTDGHEVELAGNIGGFKD
                     VEGVLTNGGEGVGLFRTEFLYMDSDHFPTEDEQFEAYKQVLEGMQGKKVVVRTLDIGG
                     DKHLSYYEFPQEMNPFLGYRAIRLCLKETDIFKTQLRALCRASVYGRLCIMFPMIATV
                     KEFRDAKAIFEEVKAELVAEGVAVSDSIEVGMMVEIPAAAVLADQFAKYADFFSIGTN
                     DLIQYSMAADRMSEHVSYLYQPYNPSVLRLVANTIKGAHEHGKWVGMCGAMAGEPHAV
                     PILLGLGLDEFSMSATQILKARKVVKSLSYEEMQGLAQECLNKDTADEVLETVKTKLG
                     E"
     gene            complement(12072..13808)
                     /locus_tag="ACZPDN_03110"
     CDS             complement(12072..13808)
                     /locus_tag="ACZPDN_03110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297643.1"
                     /translation="MSTFKRFLQYYKPYKGIFFMDLFCALIMCIVDLSFPLILSYCTG
                     KFFLKSSNEILKGVLFLGIGLLIMYVIRALCRYYVSCQGHVMGANMESDMRQDLFNQY
                     QRLSFSYYDKHNTGVMMSRVVSDLFDICEFAHHGPENLFISLFKIVGSFIILATIYWP
                     LALILLAVTIVMMVFSYSQNKKMRRTFMENRKKIGSVNASLQDSLGGIRVVQSFANED
                     IEIGKFNHSNMEFLESKKDNYRVMGTFQGGNNFFQGLLYVTIIVFGGLFIANGDLNPI
                     VLATFALYINVFVSPIEVLVEFTEMFQKGFSGFRRFEEVMNEIPEIQDSKNAKDLKNV
                     SGNIDFDHVSFQYNENEKVIDDVNLHIKAGEKIALVGPSGSGKTTLCSLLSRFYDVTN
                     GSISIDGQNIKDVTLKSLRSNIGLVQQDVYLFTGTIEQNIAYGRPNASHEEIVKAAKK
                     ANIHDFIMSLPEGYDTFCGERGTRLSGGQKQRISIARVFLKNPKILILDEATSALDNE
                     SERIIQKSLEDLSKDRTCITIAHRLSTIRNADEIVVIDATGMHERGTHDELMKLNGTY
                     AKYYKLQFEGLN"
     gene            13896..14666
                     /locus_tag="ACZPDN_03115"
     CDS             13896..14666
                     /locus_tag="ACZPDN_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1297644.1"
                     /translation="MIKILFFDIDGTLLELGQKEMYQELIIALNEVKKKGIKIFLATG
                     RPPFVVPKFHGIEFDGVLSFNGSYCFTPNELIYKNPMDKKDIQIFVDNAKKLNKAVTL
                     AGINKMGCNFDDEILEEYFMIANQHVHVLDEFNEFMEEEIYQMMIATTEDQDELILEN
                     TTTLKVARWWDKACDIIPCNGGKDIGIQKILEYFNFNKEEAMAFGDGGNDISMLKYVG
                     TGVAMDNAKDDVKAIADYITDSVQEDGIVSALKHFDIL"
     gene            complement(14706..>16366)
                     /locus_tag="ACZPDN_03120"
     CDS             complement(14706..>16366)
                     /locus_tag="ACZPDN_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1297645.1"
                     /translation="EHTSFDASYQALREEFGKSVIPFEVPIVEDGKIVGSVNIIKNKA
                     WYYSPELADKPCPVPESMQEEVALYKDQIAEAVAMGDDELMEKFFSGEEFSDAELKRG
                     VRIGVRNGEICPVYSGSAVNSIGIQRLMDLIVSYFPTYSERGMYTATTPNGVKVELLT
                     SETEVLTAQVFKTIVDPFVGRISYVKVLSGVLAADSTVVNANNGKPEKISQIYIVKGK
                     HQTAAGKLFTGDIGALVKLQNTRTNDTLCDKGKLLMADPITFDTPMYGQAVLPKTKND
                     EDRLSNGLARLQEEDPTFKVVNNPETKETVIYGVGDQHLDVIVNKLNSKFKAEVVLSD
                     PKITYRETITKTAVGEGRHKKQSGGHGQFGHVFVEFSPNPDEEEMVFAEKVFGGAVPK
                     QYFPAVETGLRECCQKGPLAGYKMVNVKTTLLDGKYHDVDSSEMAFKMAAHLAFKDAM
                     TKVRPILLEPIMNVKIRVPDEFTGAVIGDLNKRRGSIMGMTPVDSDQEIDAQVPMANM
                     SRYCVELRSMTTGLGTYTMEMDRYDPVPEPYASRVIEESKKEKE"
BASE COUNT         5150 a         3368 c         2610 g         5238 t
ORIGIN      
        1 ttccacatta tctgtgtata ctgttatttt caaccccata attataactt ggtacacgct
       61 atacactact ataaactgta ctccatttta gggtagccga taaaaatcgt gtcgtattcg
      121 tcccaatttt ctacggtgct ttttacaaga gcaatatcac gctcatcggg gttatcgtgt
      181 tcacgcacaa cacggctgtt atcgtccgtc cagttaaggt cagcgtcgct gtacggctcg
      241 gcaggctcta gctcaaataa atcgccatct gttgcggtag caatgtaatt cgccacattc
      301 tcggtattgc cggttgcgga gtagtaaaca accaatgttt ttcctgcttc atcggtaggc
      361 ggctgttctt caggtgttga ttcgctgggg gattgatttg tcgagcaggc cgccagcgac
      421 agcaccatac caagtgccat aaaaagcgaa ataatctttt tcatcgttct tcttcctttc
      481 tgtttttaat aacattcttt aaagattgca aggatttctt catgggtcat ttttttgtaa
      541 ctgccgggca taagagcaca ggaatctgcg atttctttca agtcggtatt ttcgttcacg
      601 cccaagtcct gcaaagatac aggcagtccg atttcgacaa tgaaatcttc caaagcctca
      661 atacctgcaa gggcgatttc ttcgtccgtt ttggcctctg ccgaaactcc cataacttcg
      721 attgcaaacc gtttgaattt tggcaagccg tatttgtaaa tatggcggta ataaacgggg
      781 tgcagaacgg caagcccctc gccatggttg cagtttgtgt atgcgccgag ttggtgttcc
      841 atttggtggc actcaaaatc cattcgcttg cccagtttga taacccggtt ttccgccatt
      901 gtcgccgccc acatcaaatt gcttcgtgcg ctgtagtcct gcggattctg aatagcagca
      961 cgcaggttgt ggattacact tcgcataagc gcctcggaaa tatcgtccga tacattgtcc
     1021 tcattcggct cgctgaaata aatctccata atgtgcgaca gaatatcaaa gctgccggaa
     1081 accatttgct ttttgggaac gctgtaagta taggtggggt caagcaaggc aaatttaggg
     1141 ttacattgcg gataatctct gcccgtcttg attttcaatt cctcattggt aataactgcg
     1201 ccgccgttgc actcgctgcc cgtacctgcc acagtaacaa tcacgcctag aggtaacggc
     1261 tcgaagtcaa aaacacccgg tcttttccaa aaatcttccc atacatcacc gtcataacgt
     1321 gcggcaatcg aaatcgcctt gcagcagtcc ataaccgaac cgccgccaat tcccaaaagc
     1381 agactgacat catttttctt tgccagcttc gcaccctcca ataccttcgc ataggtggga
     1441 ttcgccataa taccgctaaa ctccacaacg gtttttcctg cggcttccaa aatgtttgtt
     1501 acctcgtcat agattccgtt tttcttgatt gagccgccgc cataacatag cataacggta
     1561 tcgccgtagc ctttcgtcag gcagcaaagg tattccttta cacagccttt cccaaaaaac
     1621 acctttgtag aattttcaaa aataaagtta ttcattgtca tagtcctttc tctgatttat
     1681 tgataatttc agctaatcat tgcatttgat tttcccaaaa attcacagcg tcgtttatcc
     1741 aaccctccgc aaccgttccc gttccaagac cgaatccgtg gcgaagtccc tcgtaatgat
     1801 gaaactctgt tgggattcca agctcgttta agcgattcag ccgagcctcc attgtacgcc
     1861 agtttgcaat accgtcattt tcgcccacac aagcataggt cggtggatcg ctttcgctgt
     1921 attcgttatg acctgtgtac tgcataatca cagcccaggc ctgcggtaaa tctttttttc
     1981 cgaacgcagc cgtgccgtaa gagccaagcc aagccgccat tcttcctcct gccgagccgc
     2041 cccaaaggga atagcaatcg gtgtttactt ctaattcgtc ggcattttca aatataaagc
     2101 tgattgctct tgctaaatcc tcacaagcgg tttgtgcgcc ggggcggtag atcaaggcaa
     2161 aggcattgta gcccattttg gataattcca aagcgtgggg aaaactgtcg tgcattgcac
     2221 ctacataagc aaatccaccg cctgcgttgc ataccgcaaa ccgttcaccg ggatttcctt
     2281 tgaagaagaa caagcctgta tcttcttttg cgggatcagt tgctttttct tcatcggtat
     2341 aaatatcgta aaagattaca tcaccgtttt cagcatggct tttcatatag tttgcaatct
     2401 ctaccgtttt gtcgggggca atattgttgt accaagtgag ccccagttcg cctaatgtat
     2461 cgccgctgta atagctgctg ttcacaggga atatcagcct gccgtaatcg ccaaagacag
     2521 tgtcatttat cacatcagag attttcgtat cttgcgtata ttgttctgta tcattttgat
     2581 ttgtttgcac aatagtttct actcctttga aatctgtgtc cgattcagtg ctgttcatat
     2641 ttgcgctgca ccctgccaag aaaacggcta tgcaaatcag tgccgcaaca aaaacatttg
     2701 ctctcaaaat cgtcatctac ctttctgaat gcattataaa aaaaacgagg cctcataaga
     2761 agtctcgttt cgttcatcag tttataccca aaggttataa caatctgttt agtggtacaa
     2821 ttccttgctc accttttcgg cagcatccaa aaactgctgt acgataggcg ttggagtggg
     2881 agaatgcagg attccatagg gaatacaatg gttccactcc acaggtatta ctttcagtag
     2941 agggtgaaca ttcgcccatc cgggaatagc cagcaataca tcatttgtat tctcacaacg
     3001 attaaatata tccattccgt aaaagtcaaa atccacaata tttatttgtg ggtgattctg
     3061 ccaaaggtca tctcggagct gatcgacata gcggctccag ccacggtgca taagcagcag
     3121 attttcgccg tagaggtcag aaagttgcaa tttatcttta gttgccaatc ggtggtgaat
     3181 agaaacagca caatgaaacg gacctctatt caattccaag cccgcacaag cacgaacttc
     3241 taacattgta tcatcgaaga taccgccgac tatatcaata tttttgccta aattggctaa
     3301 aatctcccgt gcgttttcgg gggtgttctc aaagggtaca atttgaaatt taatatttgg
     3361 gtgcaaagct tgaactcgcg accaaagttc cataagaagc tgtgcagggg tgataggcga
     3421 tgaaccgata cgaataatat tgctatcttc ctgcattgca tttttggctc ttgtaatgga
     3481 atctctacaa tattgtataa tgtattttga atcctgatat agagagtgtc cggcctttgt
     3541 taagtgtagc cctctgtgtg tccgatcaaa tagtttaacg cccaacgaat cctccagcaa
     3601 attgatttgt ttgataacag ctgtaggtgt aataaaggat tcctccgccg ctttgttaaa
     3661 gctgcctgcg tccgctactc ttataaatgt ttcaagttgc gggttataca taacatttca
     3721 cctgccttat ttccaagaac gcctgaattt ttgtattccg tctattctgc tttcttcaca
     3781 tagtttttga acacgccgtt cagagatttt ctatttttgc gccacttctc tgactgatat
     3841 gtaatccatt tctatcttcc aaaaagtacg cataaccgaa taatcttaca attatattta
     3901 attcggatat gcgtattttg tcaagaagca aacctatgta tcaagctgcc tgcatttctg
     3961 caagcggctt gtgctttcag tcgggaaata taacttctgc atttataatt tcagatgctt
     4021 tattgcgaat attattcatt ttttgtaccc acagcattgc atttgacact ttaagttgtt
     4081 cattcacacc ttcacttttt gccatttgat ttacgagcca aaaaacattt cttcagcttg
     4141 atcgtttata aaggctccga ttatatagtt cggggtcaaa ctgaagacgg aaataataga
     4201 ttaatttcgg gaacaagtat tggaagtatt caatatcagc caaatcaaga tgcaaacggt
     4261 aactattata atataggtca atatgatgaa aatgatgaac aagataaacg ctatgatggc
     4321 caagactggt attataatga aggtactgtt gataatccta aatggggtgt atattacaaa
     4381 cataaatata cagaaacaga tgaaggaatt tttccactaa aagtttggta taaacaagat
     4441 gttggatata gtgaccaaaa atataatact attaacagat cctgtttttt tatgtcttat
     4501 tatgaatatt ttagactggt tttagactga gttacccagt cttagtttaa cgatttgagc
     4561 gcttttttga ggctggattt ctaaatgcac attatagttt cacataagaa aaccattcaa
     4621 tgcgaaaagc agataaaaga gcacgcctaa gtttcataaa agaaacgaaa aaaaagggaa
     4681 agccgttaag ctttacccgt tactagtttt gtctatacat caaaaaattt ctttgcgttg
     4741 ttatagaaaa tatcttctaa accctgttca tcaacgccac acattaatac tttttgcatc
     4801 tcaactgtag gatgatggaa aggagtatcc gttccaaaca ttatacgatc atgtccaacc
     4861 gtatcatacg cttctttaat ttttgtatgc ataggcatac ccgacatttc taagtaaata
     4921 tttggatatc gtctagccat ttttaatgct gcatcaatat aaactccatg accatgtccc
     4981 atatgaatca ttagtacttt acaatttgga tgtctttcag caagtaatcc aatttgccaa
     5041 ggaagtgaat aaggaggatg ccccgaatgt atacaaacag gaataccata ttcctcggct
     5101 ttatctaata caggatctac aatttcacta tccgcacaat acgcatgacg taatggctgt
     5161 agcttaatgg ctttaaaacc aagttttaca tatttatcaa tcaaatctaa acaatttggc
     5221 tctaaaggat tcacatagac acatccttca atgcgtcctg gatatttctg cattgcctta
     5281 tatgcttctt cattatccaa aacgcaaatc attgtcgttt caatttcata tgtatccatt
     5341 aacgaaatca attcgtcggc atccattttt acattggccc atccgccaat atagcctaaa
     5401 tgtgcatgtg catcgatctt tttcatgcgg tagctccttg taaagatagc actttagatt
     5461 tagccaaaac attcgcaata atcgcataaa taactagacc cataatcgtt tgagctaaaa
     5521 gtcttggtac agacgcaaag gcgactgctg ccgtaaataa aggaatctta aataatgtat
     5581 atgcgaaaca ttgaaacaaa ccaccaagac tagctccaac aatccaatcc attttttcat
     5641 tgtttccctt ttcaagtata acacccatga tatatcccat gatgaattta aagataaaag
     5701 ttggaagtac ccaaatcata gctcctgtta ataaatcact taaagcagca cctaaacctg
     5761 cagctaaagc accatccttt ttacctaaat aaagtacagc aagaaatgcc atacaatctc
     5821 ccatatgtgt atatcctccg gttgccggat ttggaagctt aattgtgaat gtacatacaa
     5881 agatcaatgc gaccattaaa gctgtttttg cgattcttct tgtcgtttca tttgtcataa
     5941 tcctgtctcc tttttaaaac ttgtccttat attagcattt tactgtattc tttaaaatat
     6001 acagttttaa cttaatttta agggacagta atttaagaaa catttaagga ttacatgttt
     6061 taataagccc atgaaaaaat tattaatgtc attcttattg attttttgta taggatgcac
     6121 ctttacattt ctcaatgaaa caaatataaa catttctgcc aaaagcacaa aagaaaccat
     6181 tatgatcgat gctggtcatg gaggatatga tgtaggagct gaaagttact atggtgatta
     6241 tgaaaaagac atcaatttag atattgcttt acttgttgga aaacaactca agtcctatgg
     6301 ctacaacgtt gtatatacac atacaagtga ttctgtttct tggccaagca ataataagaa
     6361 ggatttacaa gcacgttgtg atttagcaaa gaaaaagaat gccgatttct ttgtatcgat
     6421 acatttaaat tcaagtgaat atgaagccaa tggatatgaa atttattgtg atttcaacaa
     6481 caagaacact ttgaaagtgt ccaactccat tcttaagcaa cttgatcaat tcgattattc
     6541 aaccaatcga ggcttattag atacaaatga aacacctttg tatgttgtca aaaacaacaa
     6601 agtggatgcc atattaattg aagctggctt tatatcggat gattcggatt tatattatct
     6661 taaaaatcat acaaaaaata ttgcgacagc cattgccgca ggcatcaaaa aaagtctgaa
     6721 ctgatcagac ttttactttg cgaactgagc attatacaac ctagcataaa ctccattcat
     6781 attcaataat tcttcatgac taccttgttc aacaatatct ccatgttcaa gcacaagaat
     6841 cttatctgca ttgataattg tagataaacg gtgtgcaatc acaaaacaag tacgattttc
     6901 catcactcga tccatggcac cttgaagacg cctttcaagt cttgtatcta cactactcgt
     6961 agcttcatct aaaattaaga cttccggatt ctttaataga gcacgtgcaa ttgtcaataa
     7021 ttgtttttca ccttgactca cattacttcc ctcttcattg atcaaagtat catagccatc
     7081 cgttaaagta cgtataaagt gatggacatt agcttgtttt gcggcttcta caatttcatt
     7141 ctttaatgcc ttctcatttc catacgcaat attttgttca atactaccct caaataacca
     7201 tgtatcttgt agtactaacg caaaaagttt tcttaaatca tgatatgaca attcacgaat
     7261 atcgattcca ttgattttaa tacttccacc cttcacatca tagaaacgca acaataaatt
     7321 tgtcaatgtc gttttaccgg ctcctgtagg acctacgatg gcgatggttt gaccttgatg
     7381 tacatcaata tttacatcct tcattaatag attgtcatca tatccaaatt gtacatgatc
     7441 aaattcaata gtacgaactt catcaatttg acatttagat acaacatcct cttcaatggg
     7501 ttgatttaaa aaagtaaata aacgtgacat cgcactaaag gccgattgta cagcactcga
     7561 taattgcgat atttgtgaga ttggatcatt gatttgccaa atatatcgaa taaaagcttg
     7621 tagttgacct aatgcgattg ttccatttaa cacttgaaga catccaacaa gactagcaca
     7681 tccaatactt aaataggtaa tcaaagaaca taatggatta atcaaacttg atacaaattg
     7741 tgctttaaaa ctagatacac gcattctctc attatctttt tcaaagcgtt ttagagcatg
     7801 ttcttgttga ttataagaaa gaatctcatt atatccgtca tacaattcat tgattgtacc
     7861 atttaaatca gccaatgtat tttgttgatc atcaaataga ggctgtgatt ttttcactac
     7921 aaattttgaa agaagtgcga taacaggcaa tgccactaaa ataatgcatg tcataagcac
     7981 attgattcta aacatcataa ataatacaaa agtaaatgta catacagctg aaattactcg
     8041 tgataaagtt tgttgcagcg cattcgataa tgtatccaca tcatttgtga tacgcgataa
     8101 aagttcacct gttttttctt gatcaaaata agatacaggt aaatgatgaa tcttatcttc
     8161 aatcgcatta cgtacatcaa acatcgtttt ttgaatcgca tcggtcataa agatggccat
     8221 taaaaattgg gatatcgtct tcacaatgta gattgtaaat aagatacata aaatatgtac
     8281 aactttatct atctgaattg gctgatgtga agaaatatca tgagctaatt gggttgtgat
     8341 cataccttca acagttggat ttaatgcgaa tgtcacctgg gctgtgatga ccattaaaat
     8401 tccaaacaac aacttaaaac gataaggttt aacaaacaca aaaagatggg ataaacattc
     8461 actaaacttc attttcttag tttctttcat aatcatccaa ctccttttgt gtcaattgcg
     8521 aatgagcaat ttcttgatac acactacaat tctttaaaag ttctaaatgc gtacctaatc
     8581 caataatctg tccttcatct aagacaatga tcatatccga atccatgatt gttgaaattc
     8641 tttgggcaac cacaaaaaag attgcatcct tcacttctgg ttttaaggct tttcttagtg
     8701 tagcatccgt tttaaaatct aaagcactaa aactatcatc ataaatataa agggaaggct
     8761 tttttaagat gacacgagca atcgaaagcc tttgtttctg tccacccgaa atatttgttc
     8821 catcctctgc aatttcatcc aagtacccat tttctctttt agaaataaag tcacttgcct
     8881 gcgcaacagt tgcggcatga accatttctt catcacttgc ctctggtttt ccaaaacaaa
     8941 tattcgattg aatacttccc ttaaacatat gtgccttttg agcaacatac ccaatttggg
     9001 cgtgcaaact ctctaaatca tatttacgaa tatctacacc gttgatttta atcgatccac
     9061 gacttacatc ataaaatcga ttcattaatt ttactaacgt actcttacca gaacctgtac
     9121 ttccaatcac cgcaattttt tgtcctttat gacatgaaaa gtcaatattc tttaaaacag
     9181 cttcttcacc atttggataa gaaaaactaa catctttaaa acttaacgtt tcaattgaat
     9241 tcaataaaat acaatcttta tcattgacaa tacttggttt tgtatccaac acttccatga
     9301 tacgcttagc cgatacattg gctctaggat acatcataaa taccatacag aatatcaata
     9361 cagacatcat aacgtggaat aaatattcaa caaaggcaac tacatttcct agagtaatgg
     9421 catttgtgtt taataaagtg caacttaaat aatatacaat gactgtagcg atattcatca
     9481 aaaagaaaaa agatggttct gtacaagaca ttaatttaaa taactttgca ctttgatctc
     9541 gatagaaatc attttcatta gagaaacgct tcttttcata atcctcttta ttaaaagaac
     9601 gaatcacacg aatacctgta atattttcac gcaaaatttg attaatgtgg tcaatcgact
     9661 tttgttgatt ctcactcaat ggttcactca ctttggcaac aatgatcaca cctaaaataa
     9721 caacaggaat tgtagctaag accacataag aaagttgtaa agatatttgt aaaatcagca
     9781 taacggaaac acacatcata acaggtgtta ataatgcgga ttttaagatg acatttaaaa
     9841 ataacataat ttgataggca tcgttattgg tacgtgtaat catactggac acaccaaact
     9901 tttccatttc agctgcagaa aaagattgcg aatgcttaaa aacatcatta cgaatatcac
     9961 gcgtaatatt tgtggatatt tttgagcagc aatatccaag tagtacaact ccacttacac
    10021 cgattacact aatgaaagcc atcaagccaa aacgactata taaataagct gtatcatgtg
    10081 tttcaatacc atgatcaatc atatctgaaa tgatggttgg aattcctagt tctacaagtg
    10141 caaacccgaa tacacttata aaatccaaca aaaaccattt cttatatttc attaaatatt
    10201 tccatataag cttcatatat tccccctaca aaataaaagg gggttcaata gaaccccgaa
    10261 tctaattatt cacccaattt agttttaact gtttctaaaa cttcatcagc agtgtcttta
    10321 tttaagcatt cttgagctaa tccttgcatt tcttcatagc ttaaagattt aacaacctta
    10381 cgagccttta agatttgagt agcactcatt gagaattcat caagacctaa acctaaaaga
    10441 ataggaactg catgtggttc accagccatg gctccacaca taccaaccca tttaccgtgt
    10501 tcgtgagcac ctttaattgt attagcaact aaacgtaata cacttgggtt gtatggttga
    10561 tacaagtaag aaacgtgttc agacatacgg tcagcagcca ttgagtattg gatcaagtca
    10621 tttgtaccaa ttgagaagaa atctgcatat ttagcgaatt gatcagcaag aaccgcagca
    10681 gctggaattt caaccatcat accaacttca atgctgtcac taactgcaac accttcagca
    10741 actaattcag ccttaacttc ttcaaagata gcttttgcgt cacggaattc tttaacagtt
    10801 gcgatcattg ggaacatgat gcaaagtctt ccataaacag atgcacggca taatgcacgt
    10861 aattgagttt tgaagatatc tgtttctttt aagcaaagac gaattgcacg atatcctaag
    10921 aatgggttca tttcttgagg gaactcatag taagataagt gtttatctcc accgatgtct
    10981 aatgtacgaa caacaacttt tttaccttgc ataccttcta atacttgttt gtatgcttcg
    11041 aattgttcat cttctgttgg gaagtgatct gaatccatgt ataagaattc tgtacggaat
    11101 aatccgacac cttcaccacc attagttaat acaccttcaa catctttgaa tccaccgatg
    11161 ttaccagcaa gttcaacttc gtgtccatct gtagttacag atttttcgtt cttcaatact
    11221 tttaattctt ctttttcttt taagtaagca gctgctttag cttcatattt agcaattgtt
    11281 tcttcatccg gattgatttc aacaacacca tctaatgcat ctaatgccaa agtatcaccc
    11341 gttttacatg catcacatac acctgggcat cctacaactg cagggatttc caatgagtta
    11401 gccatgatag ctgagtgaga agttttacca ccgatttcag tagcgaaacc cttaacaaat
    11461 tcatttaact gagctgtatc acttggagtc aaatcgtgag caacgataat tgaatcttca
    11521 tcaatacttg ttaaatcagg aattgtcaat cctaataagt tacattttaa acggaaagta
    11581 acatctttaa tatcagctgc tctttcacgg aaataatcat tatccatgct ttcgaacatt
    11641 tcaaccattt gattagctac ttgttcagtc gcatattcag catttacgcc atcattttca
    11701 atcatggctt taatttgacc agctaattca gggtcaccag ccatcattaa gtgagcatca
    11761 aatacagcta attcttcgtc acttaaacgt ttagctgctc tttcttttac tccttcgata
    11821 tcttttttag tttgttctag agcatcctcg aattttttta cttcttcttc agctactcct
    11881 gctttttttt caataacaac ttttggtgtt tctaatttgt aaactttagc tacagataca
    11941 ccagcacttg ctgctatccc ttttaacatg ataaattcct cctaaaacaa gcgaaaaacg
    12001 ctttcatcta cagataaaat tttaacactt ttttttcaaa gatacaagaa aagcagacat
    12061 ttgcatgcct gctaatttaa gccctcaaat tgaagcttat aatatttcgc ataagtacca
    12121 tttaatttca tcaattcatc atgtgttccg cgctcatgca tacctgtcgc atcgattacg
    12181 acaatctcat ccgcattacg aatcgtagat aaacgatggg caattgtaat acaagtgcga
    12241 tccttactta aatcttctag actcttttga ataattcgct cactctcatt atccaaagca
    12301 cttgttgctt catccaaaat caaaatctta ggattcttca agaaaacacg tgcaatcgaa
    12361 atacgttgtt tttgtccacc tgataaacgc gtacctcttt ctccacagaa agtatcgtac
    12421 ccttcaggta aagacataat aaaatcatgg atattcgctt ttttagctgc ctttacgatt
    12481 tcttcatggc ttgcgtttgg tcttccataa gcgatatttt gttcaatcgt tccggtaaat
    12541 aaatatacat cttgttgaac taaaccaata tttgaacgaa gagattttaa agtcacatct
    12601 ttaatatttt gaccatcaat tgagatggaa ccgtttgtga catcataaaa acgagataac
    12661 aatgaacaaa gtgtagtctt accagagcca ctaggtccaa ccaaggcaat tttttctccg
    12721 gctttgatat gtagatttac atcgtcaatc accttttcat tctcattata ttggaaagac
    12781 acatgatcaa aatcaatatt tccagaaaca ttttttaaat ccttggcatt tttagaatct
    12841 tgaatttcag gaatttcatt cataacttct tcaaaacgtc taaatccaga gaaccccttt
    12901 tggaacattt ctgtaaattc aactaatact tcaatcggcg atacaaaaac attaatatat
    12961 agggcaaacg tcgcaagtac gattggattt aaatccccat ttgcgataaa cagcccacca
    13021 aatacaatga ttgtgacata taaaagtcct tggaagaaat tatttccacc ttgaaaagta
    13081 cccataaccc gataattgtc ttttttagat tccaaaaatt ccatattcga atggttaaat
    13141 ttaccaattt cgatatcctc attcgcaaaa gattgtacaa cacgaatacc acccaaagaa
    13201 tcttgaagac ttgcatttac actaccaatc ttctttctgt tttccataaa ggttcttcgc
    13261 atcttcttat tttgtgaata actaaacacc atcatcacaa tcgtcacagc taaaagaatc
    13321 aatgccaatg gccaatagat tgtagctaaa ataatgaaag atccgacaat tttaaataat
    13381 gaaataaata agttttcagg tccatgatgc gcaaactcac aaatatcaaa taaatctgaa
    13441 acgacacgag acatcataac ccctgtattg tgcttatcat aataagagaa agataaacgt
    13501 tggtattgat tgaataaatc ttgacgcata tcactttcca tgttggctcc catcacatgg
    13561 ccttgacaac tcacataata acgacacaat gctcgtatta catacataat aagtaaacct
    13621 atacctaaaa aaagaactcc ttttaaaatt tcattcgatg atttcaaaaa gaattttccg
    13681 gtacaataac ttagaatcaa aggaaacgat agatcaacaa tacacatgat aagtgcacaa
    13741 aatagatcca taaagaaaat ccctttatat ggtttatagt attgtaaaaa acgcttaaat
    13801 gtactcatat attccctccc tgatttctgt attatacaag aattttcatg atttttccaa
    13861 aataatgcta aaatatgaaa gaatggagaa tgattatgat taaaatatta ttttttgata
    13921 ttgatggaac gcttttagaa cttggtcaaa aagaaatgta ccaagaattg attattgcgc
    13981 tgaatgaagt aaaaaagaaa gggattaaaa tctttttagc cacaggaagg ccaccatttg
    14041 tcgtacctaa atttcatggc atcgaatttg atggagtact ttcttttaat ggctcctatt
    14101 gtttcacacc taacgaattg atttacaaga atcccatgga taagaaggat attcaaatat
    14161 ttgtagataa tgccaagaaa ttaaataaag ctgttactct agctggaatc aataagatgg
    14221 gatgtaattt tgatgatgaa atcttagaag aatatttcat gattgccaac caacatgttc
    14281 atgtacttga tgaatttaat gaatttatgg aagaagaaat ttatcaaatg atgattgcga
    14341 ctacagaaga tcaagatgaa ttgattctag aaaacacaac cacattaaaa gtggctcgtt
    14401 ggtgggataa agcttgtgac attattccat gtaatggtgg aaaagatata ggtattcaaa
    14461 agatattgga atacttcaat ttcaacaaag aggaagccat ggcctttggt gatggcggga
    14521 atgatatctc tatgttgaaa tatgttggta ctggtgttgc gatggataat gccaaagatg
    14581 acgttaaagc tatagctgac tacataacag attctgtaca agaagatgga atagtcagtg
    14641 cactaaaaca ttttgacatc ctatagaaaa agacagccgt tttctgctgt ctttttacat
    14701 atgtcctatt ctttttcttt cttgctctct tcaatcactc ttgaagcata aggttctggt
    14761 actggatcat aacggtccat ttccattgta tatgttccta aaccagttgt catacttcta
    14821 agttcaacac aatatctaga catgtttgcc attggaactt gtgcatcaat ttcttgatct
    14881 gaatccactg gagtcatacc catgattgaa cctctacgtt tattcaaatc accaatgaca
    14941 gctcctgtaa attcatcagg aacacgaatc ttaacgttca taattggttc taataagatt
    15001 ggacgaactt tagtcatcgc atctttaaaa gctaagtgag ctgccatctt aaatgccatt
    15061 tcacttgagt caacatcatg atatttacca tctaacaatg tagtttttac atttaccatc
    15121 ttatatcctg ctaaaggacc cttttggcaa cattcacgaa gacctgtttc aacagctggg
    15181 aaatattgtt taggaacagc tccaccaaat accttttctg caaataccat ttcttcttca
    15241 tctggattag gactaaattc tacaaataca tgtccaaatt gtccgtgtcc acccgattgt
    15301 tttttatgac gaccttcacc aaccgctgtc tttgtgatag tttcacgata agtaatcttt
    15361 ggatcactta agacaacttc ggctttaaat ttagaattta atttattcac aataacatct
    15421 aaatgttgat cgccaacacc ataaatcact gtttcttttg tttccggatt attcaccact
    15481 ttaaatgttg gatcttcttc ttgtaaacga gcaagaccat ttgataaacg atcttcatca
    15541 tttttagtct taggtaaaac agcctgacca tacattggcg tatcgaatgt gattggatct
    15601 gccatcaaca atttaccttt atcacacaat gtatcgtttg ttctagtatt ttgtagtttt
    15661 actaaagcac caatatctcc tgtaaataat ttaccagctg ctgtctggtg tttaccttta
    15721 acgatataga tttgactgat tttttctggt ttaccattat tggcattcac tacagtacta
    15781 tcagctgcaa gtacaccact caatactttt acataactaa ttcttccaac aaatgggtcc
    15841 acaattgttt tgaaaacttg tgctgttaaa acttccgttt cacttgtcaa caattccact
    15901 ttaacaccat ttggagttgt agctgtatac atacctcttt ctgaatatgt tgggaaataa
    15961 ctaacgatta aatccatcaa acgttgaata ccaattgaat taactgcact accagaatat
    16021 actggacaaa tttctccatt acgcacacca attcgtacac cacgttttaa ttctgcatca
    16081 ctaaattctt caccagagaa gaatttttcc attaattcat cgtcacccat ggctactgct
    16141 tctgcaatct ggtctttata aagagctact tcttcttgca tagattctgg aactgggcat
    16201 ggtttatccg ctaattctgg actgtaatac caagctttat ttttaatgat atttacagat
    16261 ccaacgattt taccatcttc aacgatagga acttcaaatg gaataacgct cttaccaaat
    16321 tcttcacgca acgcttgata actagcatca aagcttgtgt gttctt
//
LOCUS       DBMDWL010000026        16297 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1195_length_16297_cov_2.416214, whole genome shotgun sequence.
ACCESSION   DBMDWL010000026 DBMDWL010000000
VERSION     DBMDWL010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 16297)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16297)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16297
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1195_length_16297_cov_2.416214"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /gene="parC"
                     /locus_tag="ACZPDN_03125"
     CDS             complement(<1..1583)
                     /gene="parC"
                     /locus_tag="ACZPDN_03125"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR1297646.1"
                     /translation="MADRFARYSKYIIQERALPDARDGLKPVQRRILYAMYEDGNTYN
                     KPYHKSAKTVGNVIGNYHPHGDSSVYDAMVRMSQNWKITNPLIDMQGNNGSIDDDPAA
                     AMRYTEARLARISDFLLQDIDKDTVEWAPNFSDEKMEPTVLPARFPNLLVNGITGIAA
                     GYATNIPPHNLNEVIQASIYRISHPDCSLEELMQFVKGPDFPTGGIVMGLDGIKQAFQ
                     TGRGRVLIRSKVEVVPLRTIKQIIIHEIPYEVIKSNLVKKIDEIRLNKKIDGILDVRD
                     ESDRTGLRIVVDLKKDSNDEQILNYLYKNTDLQISYNYNVIAIVNKAPVQMSLAMMLD
                     AFISHREDVVIRRSKFDLKTKKERAHILEGLIKAVSVMDEIIHIIRKSKDKSHAKKNL
                     IERFDFTEKQAEAIVVMRLYRLTNTDVKELKAEFKQLQSDIAELEKIIRDKSELHKVM
                     VEELEKVNAEFNTPRLSKIVHEVKEIVIDEKAMISKEECMITVTRDGYIKRVSMRSYN
                     ASSDSMTQRKDTDSLVCYGA"
     gene            complement(1634..3559)
                     /gene="parE"
                     /locus_tag="ACZPDN_03130"
     CDS             complement(1634..3559)
                     /gene="parE"
                     /locus_tag="ACZPDN_03130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR1297647.1"
                     /translation="MPKQQYDESSIVILEGLDAVRKRPGMYIGSTDSKGLHHLVWEIV
                     DNAIDEALNGYGNEIAITLEKDGSVTVEDHGRGMPVGKHKSGVSTLQVIFTILHAGGK
                     FTSHGGYTSAGGLHGVGASVVNALCTWCKVTVHDGKDIWSMTFEDGGSKIGKLEKVGK
                     TNRTGSTVTFKPDPTIFKTVHFSYSTICERAQEDAFLLKGLKMIVRDKRKEEKEVIYQ
                     YEDGLKAFIDEVNQDHTPMHDSISFRGESNQIVVEGCFQYTDEYQENIFSFTNMVRTR
                     DGGSHETGAKQAFTKVFNEYARKNGFLKEKDKGFDGNDVREGLTLVINLTIPEDYLQF
                     EGQTKEKLGSPEAKPATETVVSENLRYFLEENKEIANALVRKIIKAAQARNAARKARA
                     DIRNGKGKNRSERVLSGKLASAQSKDARRKELYLVEGDSAGGSAKQGRDSKYQAILPL
                     RGKVLNTEKASLEAIEKNEELNTIIHALGAGVGANFTAEDSNYYKVIIMTDADDDGAH
                     IQNLLLTFFYRYMRELITHEMLYIALPPLYRIAKGGKEYYLYTNEELDEKKAELKNGY
                     TITRYKGLGEMNATQLWDTTMNPESRTLLCVTIENAMMAEKRVTELMGDKAELRRNWI
                     EDNVVFTLEDDYREVSA"
     gene            3887..4501
                     /gene="plsY"
                     /locus_tag="ACZPDN_03135"
     CDS             3887..4501
                     /gene="plsY"
                     /locus_tag="ACZPDN_03135"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR1297648.1"
                     /translation="MKTILCILLGYLLGSLPFALIIGKVFYHTDIRQYGSGNLGGTNA
                     GRVLGKKAGISVIVFDVLKVVLAIAIATQVSKVAGIWTGLACCIGHCYPIFAKFHGGK
                     AVSTMFGFLFSTSIFLFQDASYLLVPFGMFIIILYLFKYVSLASMCAAISSSLYITAL
                     MLGFNVESISVIIASWLMTVLVIYRHRTNIEKIKKGIENTISWL"
     gene            4567..4980
                     /locus_tag="ACZPDN_03140"
     CDS             4567..4980
                     /locus_tag="ACZPDN_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HIR1297649.1"
                     /translation="MNYLYYEHKSSQVCMVKEDERMYHACLSTHNYLNEMCLMNGSSL
                     KGRQEAFIYQMKTKKFIPVVVNISKHEVYFPTKSKKAHDCIWINYANIQNVMYYHSYC
                     RISFKDGTFLDCDHPKRIRNSMHLIFRFLNKNTPF"
     gene            complement(4989..5417)
                     /locus_tag="ACZPDN_03145"
     CDS             complement(4989..5417)
                     /locus_tag="ACZPDN_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1297650.1"
                     /translation="MNIFLKSAGKQEGTPCVSNMVQLGDFFYLSGQTGLGETIQEQTI
                     TAINKVIDVLSNYGLQLHHVVKFTVYLKNIEDKDAFLNTFKNFVDEQFPACTIVEVSN
                     LADNATVCIEGLGVNTLRHEEQMQQSSCSHDCSSCGGGCH"
     gene            complement(5442..7787)
                     /locus_tag="ACZPDN_03150"
     CDS             complement(5442..7787)
                     /locus_tag="ACZPDN_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1297651.1"
                     /translation="MAKARKKTTRKKSTKKKTQTSWLHSNLFRLCFLICMICISIIAV
                     LEFGIVGAFLKHFFEFLFGALTNGLFILNATLCAVLIFHKEQPVIRKRYMFGILFLAL
                     GLSLISSIMEFKPHPGSYGLEYVMKNAGNIIMGHLKTYSGLAGALLLCLFQSLFDYSG
                     TYVMVLLFFVLAILMFGYEKIVGYERKPKKVLKSEPVKETPVSIDRKNFIFEDEDTNE
                     SSFQIINADPVKPVQPDPVVEETKDDEPLIIGDLEPRKPSFMVDVDDEPKEKVVEKNE
                     VPVVNEGVVEKTEPKHYIRDENSDFKNYKLPKLNVLEDMERKSRSNANTITAKEKGEK
                     LIEILHEFGVEANLVQIHIGPSVTKFEIKPELGVRVNKISNLQNDIKMGLAAKDIRIE
                     APIPGKASVGIEIPNVEKTSVQMKDLMRTIPDSMKDKKLLFCLGKDLMGNNVYGELNR
                     MPHLLIAGATGSGKSVCVNAIISSILMRTKPDEVKLVLIDPKKVEFTPYNDVPHLLSP
                     VITDGDLANKALKVIVEMMDRRYDLFGELGVRNITAYNEYVLTHNDEHLKVLPRIVII
                     IDELADLMLVAAKEVEASIQRITQLARAAGIHLIVATQRPSVDVITGVIKANIPSRIA
                     FAVSQAVDSRTILDQAGAERLLGNGDMLYLPNGETSPRRIQGVFIKDEEVNNICAFVK
                     SQAMPKYDDAFIQLKDLQNQGNEAQNVTADPLYEEVKRFVIASRKASTSLIQRKFSVG
                     YSRAARLMDVLEANGIIGPSRGSKPREILVQNTLEDDIDSI"
     gene            complement(7802..9493)
                     /locus_tag="ACZPDN_03155"
     CDS             complement(7802..9493)
                     /locus_tag="ACZPDN_03155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR1297652.1"
                     /translation="MKEEKIPSLTEKIRIFALGGLDEDGKNLFCVEVDQSIYVIEAGI
                     KFPDNKESLGIEFIIQDFTYLVENKNRVAGIFITHGHDDVMGALPYLLKQVPCDVYTA
                     PLAVKEVQKMIRQEHITGVRVHGVKRNEKKRIGKRRVVFFPITHAYPGTFGLAIGTEQ
                     GYIVYSGEFIEDYDDLEDAYRADFTTISELGNEGVLVLLQESKGAERSGHTSPSHRIS
                     PKLLQVLETHENKRVFVSVYTQSVYRIQEIIDCCMANHRKMVFYTKELRDLVDRLKEI
                     GYEVDPSLVIDPADFDNSMKDVVVIISGQGKSLFKTINNIANNELESIVFDQDDVIVV
                     ASPVVPGVENEFKSMENDMYKKEGEIIVLDKNVLSMHPSKEDLKMMIFLTKPKYYIPI
                     KGEYRLLHMNSQIAVEMGYDPSRIIILDNGQVATFENRKLVSCAMELELHETMIDGKE
                     NWDMAGVVLKDRETLSTDGVMILAIGINAKTKKIINGPDVQSRGLIYLRDAEYITGDV
                     AKIMEDTINEAVENKTYDNLETRNLIRDKVSKYLYKQTAKRPMILPVILEINLNK"
     gene            complement(9533..10966)
                     /gene="miaB"
                     /locus_tag="ACZPDN_03160"
     CDS             complement(9533..10966)
                     /gene="miaB"
                     /locus_tag="ACZPDN_03160"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1297653.1"
                     /translation="MKQKPGWVLPDLKEAQKRTTNTIDRIDTFTIPEVARMMGRGKTY
                     FISTYGCQANERDSETLAGILDELGFTPNETAEGSDVIIINTCAIRQNAEEKVLGEIG
                     NFKRLYRENKDLIIGVCGCMAQEEGLVETLLTKYPQVRLLFGTHNIQELPSMLYSCMF
                     EGKKVVKIYSKEGEVYENLPVHRFGTFKAWVNIMYGCDKFCTYCIVPYTRGKQRSRKM
                     SEILKEVQELKDEGYKEITLLGQNVNAYGKDMDNEQDFATLLEEVAKIGIPRVRFTTS
                     HPWDFSEQMIDIIAKYDNIMPFIHLPLQSGDDDVLKLMGRRYTKESYLALYDKIIHTI
                     PNVAVSTDIIVGFPNETDEQFEHTLDVVRYCKYDNAFTFIFSARPGTPASRMHDSIDM
                     ETKRKRLATLNKTWNDLALEKNKAYIGRTVTVLVDGPSKKDENVYSGYTDTQKLVNFK
                     GENLQAGDFVEVKILDAKTFSLDGVAV"
     gene            complement(11026..11199)
                     /locus_tag="ACZPDN_03165"
     CDS             complement(11026..11199)
                     /locus_tag="ACZPDN_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1297654.1"
                     /translation="MAVNIDKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCG
                     SCVGTCPVGAINQ"
     gene            complement(11342..12895)
                     /gene="rny"
                     /locus_tag="ACZPDN_03170"
     CDS             complement(11342..12895)
                     /gene="rny"
                     /locus_tag="ACZPDN_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR1297655.1"
                     /translation="MDVLWIVLSGVAGLAIGSGCMVGISKAGLNKNQQKAEQILKEAQ
                     NEADSRVKQAVLDGKTQAHDLRVEAEKEIKERKQESMELENKLLRREDNLNFRDEALN
                     QKERQINEKMKSVADKLTSLDEKEKKLQEEIDKQVVVLESVAKMSSQAAREELFAIVE
                     KKMEHETIAYIKDKEDEAKATANEKAQNIIALAIERMSQQETQEKTVSVVSLPGEEMK
                     GRIIGREGRNIRCFEQATGVELNIDDTPDLITITCFNPVRREVARLALEHLIRDGRIQ
                     PGRIEDVVKKYQNEIDQEIMKAGEDAIFKLGIGRIDREIVKLIGTLKYRYSYGQNALQ
                     HSMEVAYIAGCMAAELGLNQQIAKRAGLLHDIGKALNIEQQDGSHVELGYKFCKKHGE
                     KEIVLNAIHSHHGDVEPKFLTSHLVIAADTISAARPGARKESAQAYINRLENLEKIAN
                     GFEGVANAFAIQAGREIRVLVNPEHVDDLTCVKLARDIRDKIESELTYPGQIKVNVIR
                     ETRAQEYAK"
     gene            complement(13015..13848)
                     /locus_tag="ACZPDN_03175"
     CDS             complement(13015..13848)
                     /locus_tag="ACZPDN_03175"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1297656.1"
                     /translation="MIEILKSILYGIVEGITEWLPVSSTGHMILLEEIMPMNVSSSFW
                     SMFLVVIQLGAILAVVLLYWNKIFPFRKNNKGKYVPVKSIWILWSKILVATIPAAVIG
                     LLLDDWIDAHLYNGFVVAIMLILVGVAFIYVENRNKDMRPSVDTLAALSYKDALIIGL
                     FQVVAAVLPGTSRSGATIIGGLMIGVSRAVAAEFTFFLAIPVMFGASLLKLVKFGFSF
                     SLLEFFILAFGMIVAFVVSIFVIRFLMSYIKKHDFKVFGWYRIALGIIVLVFFMVHAI
                     F"
     gene            complement(13897..14067)
                     /gene="rpmF"
                     /locus_tag="ACZPDN_03180"
     CDS             complement(13897..14067)
                     /gene="rpmF"
                     /locus_tag="ACZPDN_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1297657.1"
                     /translation="MAVQQRRNSKTRKAKRRTHYKLVKPALTRCPNCGEYKLAHKMCS
                     CGEYNGKQIVEK"
     gene            complement(14079..14588)
                     /locus_tag="ACZPDN_03185"
     CDS             complement(14079..14588)
                     /locus_tag="ACZPDN_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014657.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1297658.1"
                     /translation="MKFNRKDFLNANNHIIEIDEWIAIEENDLLHHTSVKSVPDVHVT
                     GTLEFDGMNFVSSDLSMDGTMVVLDSITNEDIDVPFETESQQTYSFVPVEDAEDQEII
                     VVKKDTIDINPEIFQAILYEAPMSITRLPRDQYPKGDGWQLLSDQDREEPKIDPRWEK
                     LNDFKLDDD"
     gene            14676..>15215
                     /locus_tag="ACZPDN_03190"
     CDS             14676..>15215
                     /locus_tag="ACZPDN_03190"
                     /inference="COORDINATES: protein motif:HMM:NF017452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1297659.1"
                     /translation="MNMKTCGIITEYNPFHQGHIYHIKKTKELTQCNCLIAIVSNHFT
                     QRGLPSLLSMEDKTKLALEAGCNLVIELPACYAAQSADYFAKYAIESLHALNIDQICF
                     GSETNNIVLLREYAKQMESTKVDPSLSMNRNLYSKDIQPNDILGIQYIKQCDKYNILP
                     QSISRKDTFKSATQTRKEYF"
     assembly_gap    15216..15225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15226..15670
                     /locus_tag="ACZPDN_03195"
     CDS             <15226..15670
                     /locus_tag="ACZPDN_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1297660.1"
                     /translation="YFNPNQTWGTYYPYLRKFLILTDTNILSTYFLVNEGIEYRLKEN
                     AKKYLKWEDFLLASISKTYTKARIQRTCMFILLQITKAQMQENNRFNQVIVAGFDSIG
                     QAFLKDKQVVTKFKELPKFLQEIELKCQYLSFDTFQARKVIYYDR"
     gene            15660..16199
                     /locus_tag="ACZPDN_03200"
     CDS             15660..16199
                     /locus_tag="ACZPDN_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1297661.1"
                     /translation="MIASLCILILACIYIYFFPFIPKRKKHYTYALLLGCPAHDDGTM
                     SSSQIKRCDLAIKAYQQNLYDTLIISGSNVKNEFIEAKVMHAYIQNKMDIPTILETNA
                     RNTFENFKFSKDIIQKQDVLILTSQTHAKRACAIARQFFSDYGCMWFKDLKPKHITRE
                     IVSRILYIKIEFLKKIGRY"
BASE COUNT         5123 a         3429 c         2250 g         5485 t
ORIGIN      
        1 gccccataac aaaccaaaga atctgtatct tttctttgtg tcattgaatc acttgatgca
       61 ttatagctac gcatagaaac acgtttaata tatccatctc gagttacagt gatcatacat
      121 tcttcttttg agatcattgc cttttcatca atcacgatct ccttaacttc atgaacgatt
      181 ttagaaagtc taggcgtatt aaattctgca ttcaccttct caagttcttc aaccatcact
      241 ttatgtaatt cagatttatc acgaataatc ttttctaatt cggcaatatc actttgaagt
      301 tgtttaaatt ctgcctttaa ttcttttaca tctgtattcg tcaaacgata tagacgcata
      361 acaacaatag cttctgcttg tttttcagta aaatcaaaac gctcaatcaa attctttttg
      421 gcatgtgact tatctttcga tttacgaatg atgtgaataa tttcatccat tacacttacc
      481 gctttgatca aaccttctaa aatatgagca cgctctttct ttgttttcaa atcaaattta
      541 cttcttcgaa tcaccacatc ttcacgatga ctgataaagg catctaacat catagctaaa
      601 gacatttgta caggagcttt attcacaatg gcgatgacgt tgtagttata tgaaatttgt
      661 aaatctgtat ttttatatag ataatttaaa atctgttcat cattactatc ttttttaaga
      721 tccaccacaa tacgaagtcc agtacgatca ctttcatcac gaacatctaa aataccatct
      781 atttttttat ttaaacgaat ctcatcaatt ttctttacaa gattcgattt gatcacttca
      841 taaggaatct catgaataat aatttgttta attgttcgca aaggtactac ttctactttt
      901 gaacgaatca aaacacgtcc acgacctgtc tgaaaagctt gtttgatacc atcaagcccc
      961 atcacaattc cgccagttgg aaagtctgga cctttcacaa actgcatcaa ttcttctaat
     1021 gaacaatctg gatgcgaaat acgataaata cttgcttgaa tgacttcatt caaattgtgt
     1081 ggaggaatat ttgtcgcata tcctgccgca ataccagtaa ttccattgac taataaattt
     1141 ggaaaacgtg caggaagtac ggttggttcc attttttcat ccgaaaagtt gggtgcccat
     1201 tccactgtat ctttatcaat atcttgtagt aaaaaatcac taattctagc taagcgtgcc
     1261 tctgtataac gcatcgcagc agctggatca tcatcgatag atccattatt tccttgcata
     1321 tcaattaaag gattggtaat tttccaattt tgtgacatac gaaccatcgc atcataaaca
     1381 gaactatctc catgagggtg ataattacca attacattac ctactgtttt tgcggattta
     1441 tgatatggct tattgtatgt atttccatct tcatacatcg catataaaat acgtctttga
     1501 actggcttta gtccatccct agcatcaggt aaagcacgtt cttgaattat atattttgaa
     1561 tatctggcaa aacgatccgc catgatatct tctaaagaat tctttaaaac attatgtttt
     1621 acttcttttt ctttcacgca cttacctccc tataatcatc ttctaatgtg aatactacat
     1681 tgtcttcgat ccaattacga cgaagttctg ccttatctcc cattagttca gtcactcttt
     1741 tttcggccat catcgcattt tcaatggtca cacaaagaag agttctagat tctggattca
     1801 ttgtcgtatc ccaaagctga gtagcattca tttcaccaag acctttatat cgtgtaatgg
     1861 tatagccatt tttcaattcc gcttttttct catccaactc ttcatttgta tataaataat
     1921 attcttttcc acccttggca atacgatata gtggtggaag cgcaatatat aacatttcat
     1981 gcgtgatcaa ttcacgcata tagcgataga agaatgtcaa taataagttt tgaatatgtg
     2041 ctccatcatc atccgcatcg gtcataataa taactttata gtagtttgaa tcttcagccg
     2101 taaaatttgc acctacacct gcccctaagg catgaatgat cgtattcaat tcttcatttt
     2161 tttcaatggc ttcaagactt gccttttctg tatttaaaac cttaccgcgc aaaggtaaaa
     2221 ttgcttgata ttttgaatca cgcccctgtt ttgcagaacc acccgcagaa tcaccttcga
     2281 ctagatataa ttcttttctt cttgcatctt tactttgagc cgatgccaac tttccagata
     2341 aaactctttc cgaacgattt ttcccttttc cattacgaat atctgcacgc gcttttcttg
     2401 cagcatttcg agcttgtgca gccttgatga tcttacgtac tagagcattg gcaatctctt
     2461 tattttcttc taagaaataa cgaagatttt ctgatacaac cgtttcggtt gcaggtttag
     2521 cttctggact ccccaatttc tctttggtct gtccttcaaa ttgtaaataa tcttctggaa
     2581 tggttaaatt aatgactaat gttaatcctt ccctaacatc atttccatca aagcctttat
     2641 ctttttcttt taagaaacca tttttacgtg catattcatt aaatactttc gtaaaagctt
     2701 gtttagcacc cgtttcatga gagcccccat cacgagtacg aaccatattc gtaaatgaga
     2761 aaatgttttc ttgatactca tctgtatatt ggaaacatcc ttcaacgacg atttgattac
     2821 tttcacctct aaagctaatt gaatcatgca ttggtgtatg atcttgattt acttcgtcaa
     2881 taaatgcttt taatccatct tcatattgat agataacttc tttttcttct ttgcgcttat
     2941 cacgaacaat catttttaat ccttttaata aaaaggcatc ttcttgagct ctttcacaaa
     3001 tggtagaata tgaaaaatga accgtcttaa agatagtcgg atccggttta aaggttaccg
     3061 tacttccagt acgattggtt tttcctactt tttcaagttt tccaatctta cttcccccat
     3121 cttcaaatgt catactccaa atatcttttc catcatgtac tgtaacctta caccatgtac
     3181 atagagcatt tacaacactt gcacctacac catgtaatcc tccagcactt gtatatcctc
     3241 catgacttgt aaatttacca cctgcatgta gaatagtaaa aataacttga agtgtactta
     3301 ctcctgattt atgttttcca acaggcattc ctcgcccgtg atcttcaact gttacactac
     3361 catctttttc tagggtaatc gcaatttcat ttccatagcc atttaaagcc tcgtcaatcg
     3421 cattatcaac aatttcccat acaagatgat gcaaaccttt tgaatccgta gatccaatat
     3481 acatacctgg acgctttctt acggcatcca agccttctaa aataacgata ctactttcat
     3541 catattgttg ttttggcata ctatccctac tttccaacat aatctttatt atatcaacaa
     3601 atcaaaaaaa gtgcacataa attccgtcaa ttcgatttga ttatacacgc tatatataca
     3661 ttattcatgt caaattaaaa aagtgctttc ccaccctatt cttaaactta tatttacctt
     3721 taccatgtcc actttttcgg caccaatgtc cacttttttg tgttcaatgt ccactttttt
     3781 gatatacatt taataactac atcatttgtg tcaaataatt atttttttca tccaattctc
     3841 gactaagtag atcaaaactg ctaaaataag tttcgaggtg gaagaaatga aaactattct
     3901 atgtatttta ttaggttact tattaggatc tttacccttc gctttgatta ttggtaaagt
     3961 tttctatcat acagacatac gccaatatgg ctcaggcaat ttaggcggaa caaacgccgg
     4021 aagagttcta ggtaagaaag ccggcatttc tgttattgta tttgatgttt taaaagtagt
     4081 acttgcgatt gcgattgcga cacaagtatc taaggttgca gggatctgga caggacttgc
     4141 atgctgcatc ggacattgtt atcctatttt cgcaaaattc catggaggca aagccgtcag
     4201 tacaatgttt ggcttcttat tttctacaag cattttctta tttcaagatg caagctactt
     4261 acttgtacca tttggcatgt ttataattat cctatactta tttaaatatg tatctctagc
     4321 aagtatgtgt gcagccattt caagcagttt atacattaca gccttaatgc taggattcaa
     4381 tgttgaaagc atttctgtta ttatcgcaag ctggcttatg actgtactcg ttatttatag
     4441 acatcgtaca aatattgaaa aaatcaaaaa gggtatagaa aatacaatct cgtggttata
     4501 gaaagttttt taataaactt tctttttttt atgtcttttt cacattggac ccgtattata
     4561 gataatatga actatttata ctatgaacat aaatcaagcc aagtctgcat ggtaaaagaa
     4621 gatgaaagaa tgtatcatgc ttgtttaagc acacataatt acttaaatga aatgtgtctt
     4681 atgaatggat ctagcctaaa aggaaggcaa gaagccttta tctatcaaat gaagaccaaa
     4741 aaattcattc ccgttgttgt gaatatttca aaacatgaag tatactttcc aaccaaatca
     4801 aaaaaagcac atgactgtat atggatcaat tatgccaata tccaaaatgt catgtactat
     4861 cattcctatt gtagaatttc ttttaaggat ggtaccttct tagattgtga ccatcccaaa
     4921 agaattcgca attcaatgca ccttatattt cgctttttaa ataaaaatac accgttttaa
     4981 ccggtgtatt agtgacatcc tccaccacat gatgaacaat catgtgagca acttgattgt
     5041 tgcatttgtt cttcatgacg aagtgtattt acgccaagtc cctcaatgca tactgtggca
     5101 ttatctgcta aattcgatac ttctacaatt gtacatgcag ggaattgttc atctacaaag
     5161 tttttaaatg tattcaagaa tgcatcttta tcctcgatat tctttaaata cactgtaaac
     5221 ttcacaacat gatgaagctg taaaccatag tttgataaaa catcgattac tttattaata
     5281 gctgtaattg tctgttcctg aattgtttct ccaaggcctg tttgaccact taaataaaag
     5341 aagtctccca attgaaccat atttgaaaca catggtgttc cttcttgttt tcctgctgat
     5401 tttaaaaata tattcatagt taaaattcct ttcttcttct attatataga atctatatca
     5461 tcttctaaag tattttgcac taaaatttct ctaggcttac tacctcttga aggcccaatg
     5521 ataccatttg cctctaaaac atccatcaat cttgcagctc tagagtatcc tacagagaac
     5581 tttctttgaa tcaaagacgt gctcgccttc cttgatgcga tcacaaaacg tttcacttcc
     5641 tcatataaag gatctgccgt aacattttgt gcctcattac cttgattctg taaatctttt
     5701 aattgaataa aggcatcatc atattttggc attgcctgcg atttcacaaa tgcacaaata
     5761 ttattaactt cttcatcctt aataaagaca ccctgaatac gtctaggact cgtttctcca
     5821 tttggcaaat acaacatatc accatttcca agtaaccgct ctgctccagc ttggtccaaa
     5881 atcgttcttg aatccacagc ttgtgatacc gcaaaggcaa tacgacttgg aatatttgcc
     5941 ttaattacac ccgtaataac atccacactt ggtctttgtg tggcaacaat caaatgaatt
     6001 cctgcagcac gtgcaagctg tgtaattctt tgaatgcttg cttccacctc tttagctgcc
     6061 accaacatca aatcagcaag ctcatcaata ataatgacaa tacgaggtaa aacctttaag
     6121 tgttcatcat tatgcgttaa aacatattca ttatatgccg taatattacg aacacctaat
     6181 tcaccaaata aatcataacg acgatccatc atttctacaa tgaccttcaa cgccttattg
     6241 gcaagatctc catctgtaat tactggagat aataaatgtg gtacatcatt atagggtgta
     6301 aactctactt ttttaggatc aatcaaaacc aatttcactt catccggttt tgtacgcatt
     6361 aatatcgaag aaataatcgc gtttacacat accgatttac cactacctgt agctcctgca
     6421 atcaataagt gcggcatacg atttaattca ccgtagacat tatttcccat taaatctttt
     6481 cctaaacaga aaagtaactt tttatctttc atcgaatccg gaattgtacg catcaaatcc
     6541 ttcatctgaa cacttgtctt ttcaacattt ggaatctcaa ttccaacact tgccttacca
     6601 ggaattggag cttcaatacg aatatcttta gctgcaagac ccatcttgat gtcattttgt
     6661 aagttactga ttttattaac acgaacacca agttctggtt tgatttcaaa ctttgttaca
     6721 cttggcccaa tatgaatctg aacaagattg gcttcgacac caaattcatg taggatttca
     6781 atcagttttt cccctttttc cttagccgta attgtattcg cattggatct tgattttctt
     6841 tccatatcct ctaaaacatt caattttgga agcttatagt tcttaaaatc cgaattttca
     6901 tcccgaatat aatgcttagg ctccgttttt tctacaaccc cttcattaac gacaggcact
     6961 tcattttttt caacaacttt ttcttttggc tcatcatcca catccaccat aaagctaggt
     7021 tttctaggct caagatctcc aataatcaaa ggttcatcat cctttgtttc ttcaacgaca
     7081 ggatctggtt gtacaggttt tactggatcg gcattgatga tttgaaaact cgattcattt
     7141 gtatcttcgt cttcaaatat gaaattttta cgatcaatcg ataccggagt ttccttgact
     7201 ggttcacttt tcaaaacctt tttaggcttt ctttcatatc ccacaatctt ttcataacca
     7261 aacatcaata tcgcaagcac aaaaaataat agtaccatga cataggttcc agaataatcg
     7321 aataatgact gaaaaaggca tagcaacaaa gcgcctgcta atccagaata tgttttcaaa
     7381 tgacccataa taatattacc tgcatttttc ataacatatt ctaagccata actacctgga
     7441 tgtggtttaa actccataat cgaagaaatc aaacttaagc caagtgccaa aaataagatt
     7501 ccaaacatat agcgcttacg aatcacaggc tgttccttat ggaaaataag aactgcacac
     7561 aaggtagcat tcaaaataaa taatccattt gtcaaagctc caaataagaa ttcaaagaaa
     7621 tgttttaaaa aagcgcctac aatgccaaat tctaaaacgg caataatact aatacatatc
     7681 atacaaataa ggaaacacaa acgaaataag tttgagtgca accaagacgt ttgtgttttc
     7741 ttcttcgtag atttttttcg tgttgttttt tttctggctt ttgccatact taccccactt
     7801 tctacttatt taagttaatt tctaaaataa ccggtaaaat catcggtctt tttgcggttt
     7861 gtttataaag atatttagaa actttatcac gaatcaaatt tcgtgtctct aaattatcat
     7921 acgttttatt ttcaaccgcc tcattgatcg tgtcttccat aattttggca acatccccag
     7981 tgatatattc ggcatcgcgc aaatagatca atccacggct ctgtacatct ggaccattga
     8041 tgatcttttt cgttttcgca ttaataccaa tcgccaaaat cataacacca tccgtactta
     8101 atgtttcacg atcttttaag acaacaccag ccatatccca gttttctttt ccatcgatca
     8161 ttgtttcatg aagttctaat tccatagcac aagaaaccaa tttacgattt tcaaaagtag
     8221 caacctgccc attatctaaa ataatgatac gacttggatc atatcccatt tcaaccgcaa
     8281 tctgtgaatt catatgcaac agacgatatt cccctttgat tggaatatag tattttggtt
     8341 ttgtaaggaa gatcatcatc ttcaaatctt ctttactagg gtgcatactc aaaacattct
     8401 tgtctagaac aatgatttca ccctcttttt tatacatatc gttttccatg ctcttgaatt
     8461 cattttcaac accaggaaca actggacttg cgaccacgat cacatcatcc tgatcaaata
     8521 caatcgactc taattcatta ttggcaatat tgttgattgt tttaaacaaa gacttacctt
     8581 gtccagaaat gatgactaca acatctttca tggaattatc aaaatcagct ggatcaataa
     8641 ctaaagatgg atctacttca tagccaattt cctttaaacg atccactaaa tcacgtaatt
     8701 ccttcgtata gaaaaccatc ttacgatgat ttgccataca acaatcaatg atttcctgaa
     8761 tgcgatacac actttgtgta tatacactga caaaaacacg tttattttca tgtgtttcta
     8821 aaacctgtaa aagctttggc gaaatacgat gacttggaga agtatggcca cttctttcag
     8881 ctcccttact ttcctgcaac aataccaata caccttcatt accaagttcc gatattgttg
     8941 taaaatcagc tctataagca tcttctaaat catcataatc ctcaatgaac tcacctgaat
     9001 atacgatata tccttgttca gtaccaatcg caagaccaaa cgttccagga tatgcatggg
     9061 taattgggaa gaagacaact cttctttttc caatgcgttt cttttcatta cgcttaacac
     9121 catgaacacg cacacccgta atgtgttcct gtcgaatcat tttttgtact tcttttacag
     9181 ctagaggcgc cgtatacaca tcacaaggga cttgttttaa taaatatggt aaagcgccca
     9241 taacatcatc gtgtccatgc gtaataaaaa tacctgcgac acgattttta ttttccacta
     9301 aatatgtaaa atcttgaata ataaattcaa tacctaacga ttctttgttg tctgggaatt
     9361 tgattccagc ttcaattacg tagatggatt gatccacctc cacacagaat aagttctttc
     9421 catcctcatc taaaccacca agggcgaaga tacgaatttt ttctgtcaat gaaggtattt
     9481 tttcttcttt catattatct ccttttaaat agtattattt ttctaaatta tatcacacag
     9541 ctacgccatc taaagagaaa gttttcgcat ctaagatctt aacttcaaca aaatctcctg
     9601 cttgaagatt ttctccttta aagttcacta gtttttgcgt atctgtatat cctgaataca
     9661 cattttcatc tttcttacta ggaccatcca caagtactgt cactgtacga ccaatataag
     9721 ctttattctt ttctaaagcc aaatcattcc atgttttatt taatgtagcc aaacgttttc
     9781 tctttgtctc catatcaatc gaatcatgca tacgactggc tggtgtacca ggtcttgcag
     9841 agaaaataaa cgtaaaggca ttatcatatt tacaataacg aacaacatct aatgtatgtt
     9901 caaattgttc atctgtttca tttggaaatc caacaatgat atctgttgaa acagcaacat
     9961 ttggaattgt atggatgatc ttatcatata aagccaaata agattctttt gtgtaacgac
    10021 gacccattaa tttaagtaca tcatcatctc cagattgtaa aggcaagtga ataaacggca
    10081 tgatattatc atactttgcg ataatatcaa tcatttgttc agagaaatcc caaggatgac
    10141 tcgttgtaaa acgaacacga ggaattccaa tcttagccac ttcctctaat aaagtcgcaa
    10201 aatcttgctc attatccata tctttaccat atgcatttac attttgccca agcaatgtaa
    10261 tttctttata tccctcatct ttcaattctt gaacttcttt taagatttca gacatctttc
    10321 tagaacgctg ttttccacga gtataaggaa caatacaata tgtacagaat ttgtcacaac
    10381 catacataat attgacccaa gccttaaatg tgccaaaacg atgtactggt agattttcgt
    10441 aaacctctcc ttctttcgaa tagattttta cgaccttttt accttcaaac atacaactat
    10501 aaagcatact tggaagttct tgaatattat gtgttccaaa caacaagcgt acttgaggat
    10561 attttgtaag caaagtttca accaaacctt cttcttgcgc catacaacca catacgccaa
    10621 tgatcaaatc tttattttcg cgatataaac gcttaaagtt accaatctca cctaagactt
    10681 tctcttcggc attttgacga atcgcacacg tattgatgat aatgacatca ctgccttcag
    10741 ccgtttcatt tggtgtaaat cctagctcat ctaaaattcc agccaatgtt tcgctatcac
    10801 gttcattagc ttgacaacca tatgttgaaa taaaatatgt ttttcctctt cccatcatac
    10861 gagctacttc aggaatcgtg aaagtatcaa tacgatcaat tgtatttgta gttcgttttt
    10921 gtgcttcttt taaatcgggt aaaacccatc ctggtttttg tttcatatgt tctcctttcg
    10981 aaaaaaaatg ccaagcatta agcttggcaa aagtttaaat ttgtattact ggttgattgc
    11041 tccaactggg caagttccta cgcatgaacc gcagtcgatg caagttcctt cgtcacaaac
    11101 tgattttcct tcatcatcta atgaaagagc tccaacagga catcctccta cacaagcacc
    11161 acatccgatg caagcatctt tatcgatatt tactgccatt gtcatttcct ccttgtttaa
    11221 taatttatac tcaaattata ctatatccta gaagtgaatc aaggcagttt agtaagtgca
    11281 ggcttaccat aattcattta ccaaactaaa aaattgaagt cgggactaaa gcccgacatt
    11341 tttattttgc gtattcctgg gcacgtgtct cacgaataac atttactttg atttgacctg
    11401 gatatgtcaa ttcagattca atcttatctc gaatatcacg agctaatttt acacaagtca
    11461 agtcatcaac gtgttctgga tttacaagca cacgaatttc acgacctgcc tgaatcgcaa
    11521 atgcattcgc aacaccttca aatccattcg caatcttttc taagttttca agacggttga
    11581 tataagcttg agcactttct ttacgagctc caggtcttgc agcactaatt gtatcagccg
    11641 caataaccaa atgacttgtt aagaattttg gctctacatc accatggtgt gagtgaatcg
    11701 cattcaatac aatttctttt tcaccatgct ttttacagaa tttgtatccc aattcaacat
    11761 gagatccatc ttgttgctca atattcaatg ccttaccaat atcatgcaat agacctgcac
    11821 gtttggcaat ttgttgattc aatccaagtt cagctgccat gcatcccgca atataggcaa
    11881 cttccataga atgctgtaat gcattttgtc cataagaata acgatacttc aatgtaccaa
    11941 ttaattttac gatttcacga tcaattcgac caatacccaa tttgaagata gcatcttctc
    12001 cagccttcat gatttcttga tcaatttcat tctggtattt ctttacaaca tcctcaatac
    12061 gtcctggctg aattcttcca tcacgaatca agtgttctag cgcaagtcga gccacttcac
    12121 gacgcaccgg attaaaacat gtgatcgtaa tcaaatctgg tgtatcatca atattcaatt
    12181 caacaccagt agcttgttca aagcaacgaa tattacgacc ttcacggcca ataatacgtc
    12241 ccttcatttc ttcaccaggt aaagacacaa cactgactgt tttttcctgt gtttcttgtt
    12301 gagacatacg ctcaatagct aaggcgatga tattttgcgc tttttcattg gcagttgcct
    12361 ttgcctcatc ctctttatct ttgatatatg cgattgtttc atgttccatt ttcttctcta
    12421 caatcgcaaa caattcttct ctggctgcct ggcttgacat tttggcaaca ctttctaaaa
    12481 caacaacttg tttatcaatt tcttcttgta atttcttttc tttttcgtct aatgatgtca
    12541 gtttgtcagc aacactcttc atcttttcat taatctgacg ctctttttga tttaacgctt
    12601 catcacgaaa attcaagtta tcttctctac gcaacaactt gttctctaat tccattgatt
    12661 cttgtttacg ttctttaatt tctttttcag cttcaacacg caaatcatga gcctgtgtct
    12721 ttccgtctag cacagcttgt ttcacgcgag agtctgcttc attttgagct tcttttaaaa
    12781 tttgttctgc tttttgctga ttcttattta aacctgcttt ggaaattccg accatacagc
    12841 cagatccaat cgcaagacct gcgacaccag acaaaacgat ccatagtaca tccatactat
    12901 ttcctccttt tcgtcctttg ttctaggtaa aaccaaaaat attatacacg aaaacaggca
    12961 taaagtctat cgtgtcaaat cctaaaataa aagatgtttg tctagacaaa caccttagaa
    13021 aatggcatga accataaaga aaactaaaac aattatacca agcgcaatac gataccatcc
    13081 aaacactttg aaatcatgct ttttaatata agacattaag aaacgaatca caaaaataga
    13141 aacgacaaat gccacaatca ttccaaacgc aagaataaag aattccaaca aactaaatga
    13201 gaatccaaat ttcactaatt ttaatagact cgcaccaaac ataactggaa tcgctaaaaa
    13261 gaaagtaaat tcagccgcta cagcacgact tacaccaatc attaatccac caatgatcgt
    13321 tgctccagat cttgaagttc caggtaaaac agctgccaca acttgaaata agccaataat
    13381 gagcgcatct ttataagata aggcagcaag tgtatctaca cttggacgca tatctttatt
    13441 gcgattttct acatagataa acgcaacacc cactagaatc aacataatcg caaccacaaa
    13501 tccattatat aaatgtgcat cgatccaatc atctaataat aaaccaataa cagctgctgg
    13561 aattgtagcc accaaaattt ttgaccataa gatccaaatg gatttaacag gaacatattt
    13621 tcctttatta ttttttctaa aaggaaatat tttattccaa tacaataaaa caaccgctaa
    13681 gattgcaccc aattgaatca cgacaagaaa catactccag aatgaacttg atacattcat
    13741 cggcataatt tcttccaata aaatcatatg acctgttgag ctaacaggaa gccattccgt
    13801 aattccttca acaatgccat acaatataga ttttaaaatc tcaatcatat tttcctccat
    13861 actacagaaa aagccaggat ttacctggct aatttactac ttttcaacga tttgtttgcc
    13921 gttgtattcg ccacaagagc acatcttgtg agcaagttta tattcaccac aatttggaca
    13981 acgagtcaac gctggtttca ccaatttgta atgtgttcta cgttttgctt tacgtgtttt
    14041 tgaatttctt ctctgttgaa ctgccatgct tgcacctcct agtcatcatc cagtttgaaa
    14101 tcatttaatt tttcccatcg tggatcaatt tttggctctt ctctatcttg atccgataaa
    14161 agctgccaac catctccctt tggatattga tcacgaggta aacgagtgat actcattgga
    14221 gcctcatata atatcgcttg gaaaatctct gggttaatat cgattgtatc ttttttcacc
    14281 acgataattt cctgatcctc tgcatcctct acaggaacaa aagaatacgt ttgttgcgac
    14341 tccgtctcaa atggaacatc aatatcttca tttgtgatgg aatctagaac aaccattgtt
    14401 ccgtccatac tcaaatcact cgatacgaaa ttcattccgt caaactctaa tgtacccgta
    14461 acatgtacat ctggaacaga cttgacagaa gtatgatgaa gcagatcgtt ttcttcaatc
    14521 gcaatccatt catcgatctc aataatatgg ttatttgcat ttaagaagtc ttttctatta
    14581 aatttcacga tcttcacctc atgtcgcatt tgatattata ataaaagtaa ttcataaagt
    14641 caatatttcc acaagaaaaa gattctgtta tcataatgaa tatgaaaaca tgcggaatta
    14701 ttacagaata taatcctttt caccaaggac atatttatca tatcaaaaaa acgaaagaat
    14761 taacacaatg taattgctta attgcgattg tgtccaatca ttttacccaa agaggcttac
    14821 ctagcctttt aagtatggaa gataagacta aacttgctct agaagccggt tgcaaccttg
    14881 tgattgaact tcctgcttgt tatgcagcac aaagtgccga ttactttgcc aaatatgcaa
    14941 ttgaatcttt gcatgcgtta aatatcgatc aaatttgttt tggctcagaa acaaacaaca
    15001 ttgtcttgct aagagagtat gccaaacaaa tggaatcgac aaaagtagat ccatccttaa
    15061 gtatgaatcg aaatttatat tcaaaagaca tacagccaaa tgacatctta ggcattcaat
    15121 atatcaaaca atgtgacaag tataatattc ttccacaatc catttctcga aaggatactt
    15181 tcaaaagtgc cacacaaaca cgtaaagaat actttnnnnn nnnnnctact tcaatcccaa
    15241 tcaaacttgg ggcacttact atccatatct tagaaaattc ttgattttaa ctgacacaaa
    15301 tattttatcc acctattttc tagtcaatga aggtattgaa tatcgcttaa aagaaaatgc
    15361 caagaaatat ttgaagtggg aagattttct gttggcatcc atttctaaaa catacacaaa
    15421 agctcgaatt caaagaactt gtatgtttat tcttctacaa atcacaaaag cacagatgca
    15481 agaaaacaat cgttttaatc aagtgatcgt agcaggattt gattcgattg gacaagcatt
    15541 tcttaaagac aaacaagtcg tcacaaaatt taaagagctt cctaaattcc ttcaagaaat
    15601 tgaactcaaa tgtcaatatt tatcttttga cacatttcaa gctagaaagg tgatttatta
    15661 tgatcgctag tttatgcatt ttaatccttg catgtatata catttatttc ttccccttta
    15721 tcccaaaaag aaagaaacac tatacatatg cattgctact tggatgtcct gctcatgatg
    15781 atggaacgat gtcttcaagt caaattaaaa gatgtgattt agccataaaa gcatatcaac
    15841 aaaaccttta cgacacattg attatttccg gttccaatgt aaaaaatgaa tttatcgaag
    15901 ccaaggttat gcatgcttat attcaaaata aaatggatat cccaaccatt ctagaaacaa
    15961 acgcaagaaa tacatttgag aatttcaaat tttcaaaaga tattattcaa aaacaagatg
    16021 tattgatttt aacatcacaa actcacgcca aaagagcttg tgccattgcc agacaattct
    16081 tttcagatta tggctgtatg tggtttaaag atttaaaacc taaacacatc acaagagaaa
    16141 tcgtttctcg tattctttat attaaaattg aatttttaaa aaaaatagga aggtactaaa
    16201 taaaatggta cccataagga ggacaacaac gtgacagttg tgatactcac ttgtgggtat
    16261 cttttttcgt tgttttaaaa taaatatgtc aagagcc
//
LOCUS       DBMDWL010000027        15979 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1227_length_15979_cov_3.815531, whole genome shotgun sequence.
ACCESSION   DBMDWL010000027 DBMDWL010000000
VERSION     DBMDWL010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15979)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1227_length_15979_cov_3.815531"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            367..1626
                     /locus_tag="ACZPDN_03205"
     CDS             367..1626
                     /locus_tag="ACZPDN_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1297662.1"
                     /translation="MNKRFLTIAAALSMGVALTACSGGGDEGKTGGSSVANADKPLVW
                     YNRQPSNSSTGEIDMDALNFNKDTYYVGFDANQGAELQGQMIKDYIEKNIDTLDRNGD
                     GVIGYVLAIGDVGHNDSIARTRGVRKALGTAVEKDGEIVSDPAGINNDGKSKSVQDGS
                     LEINGKTYTVRELASQEMKNSSGATWDAATAGNTIGTWTASFGDEVDVVASNNDGMGM
                     SMFNAWSKENKVPTFGYDANSDAVAAIAEGYGGTISQHADVQAYLTLRVLRNALDGVD
                     IDTGIGTEDDAGNVLSSDVFTYNKDQRSYYALNVAVTADNYKDFLDSTVTYAPVSNQL
                     DEKDHAKKSVWLNIYNASDNFLSSTYQPLLEKYDDLLNLDVEYIGGDGQTESNITNRL
                     GNPDKYDAFAINMVKTDNAASYTGILK"
     gene            1721..3229
                     /locus_tag="ACZPDN_03210"
     CDS             1721..3229
                     /locus_tag="ACZPDN_03210"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1297663.1"
                     /translation="MADKNEELVLSIRGMSKSFGRNRVLDHINLDLKPGTVMGLMGEN
                     GAGKSTMMKCLFGTYQKDEGTIFLEGKEINFSGPKEALENGIAMVHQELNQCLERNVK
                     DNLFLGRYPTNAFGVVDEDRMKKKSSELFRKLGMTVNLDTPMRNMSVSQRQMCEIARA
                     ISYNSKVIVLDEPTSSLMTQEVNKLFDMMRMLKKQGISLIYISHKMDEIFEICDEVSV
                     LRDGKMVMTKPTSETNMNELIAAMVGRSLDNRFPPVDNVPGEPMLSIQHVSTKYEPYL
                     KDITFDVKKGEIFGLYGLVGAGRTELLETIFGIRTRASGRVYFDNKLMNFNSAKEAIE
                     HGFALITEERKANGLFLKCDLTFNTTIANLPSYMSKVALSDNKMVQATNKEIKTMRTK
                     CMGPNDAITSLSGGNQQKVIFGKWLERSPNVFMMDEPTRGIDVGAKYEIYELIIKMAK
                     QGKTIIVVSSEMPEILGITNRIGVMSNGRLAGIVNTKDTNQEELLRLSAKYL"
     gene            3241..4899
                     /locus_tag="ACZPDN_03215"
     CDS             3241..4899
                     /locus_tag="ACZPDN_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1297664.1"
                     /translation="MTNTVTNILSRDEELALRKPIEDYVGKIQAEIDELREEGTNKVL
                     EYSEEIEVLKRDKIYTKSEKEARISELNGKLQKAKEIEAKNKQPINDKIAVAEKYLKD
                     HYQTDYYNKVVESNKYEVQKAKADYDKKLKELEKEHKDILSGLSDKEEIKEENFIYKN
                     RLFDAKLEYTKNMQEAKDRKHDAFTFEYHLIDLLKMSNFSFAEKQAQKIENYKYSFNT
                     RDFLLKNGLYIAIILVFIFLCIITPIKKNGLQLFTVNNILSIMQQASPRMFLALGVSG
                     LIMLAGTDLSVGRMVGMGMTAATIIMHNGPNTGSVFGHVFDFSTMPVGGRILLALVVC
                     VVLCTMFTTIAGFFAAKFKMHPFISTMANMLVIFGLVTYSTKGVSFGGIEQSIPGKII
                     PKVGGFPTIILWAVAAVVIVWFIWNKTKFGKNLYAVGGNPEAASVSGISVFKVTLGAF
                     IMAGILYGFGSWLECIRMIGSGSAAYGQGWEMDAIAACVVGGVSFTGGIGKISGVVVG
                     VLIFTALTYSLTILGIDTNLQFVFSGIIILIAVTLDCLKYVRKN"
     gene            complement(4916..5827)
                     /gene="pfkB"
                     /locus_tag="ACZPDN_03220"
     CDS             complement(4916..5827)
                     /gene="pfkB"
                     /locus_tag="ACZPDN_03220"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR1297665.1"
                     /translation="MIYTITFNPSLDYVIQVDHFKPGIINKTKFEEILPGGKGINVSI
                     VLSNLGHESTALGFMAGFTGKEIEHKLESYGAKTDFIHVKKGLSRINVKMKSDEETEI
                     NGMGPDIQPEDIQELFNQLDELTEKDVLVISGSIPSTLPDTMYEQIIERVQEKKIKVV
                     VDATNNLLLKVLKYKPFLIKPNNHELGEIFNVELNTRDEVVPYAKKLQEMGAQNVLIS
                     MAGQGAVLVSDNNEVIQSNAPKGTVVNSVGAGDSMVAGFLAGYLETGNYEKAFINGLC
                     CGSASAFQVGLATKEDVENIKKTLSEN"
     gene            5986..7248
                     /locus_tag="ACZPDN_03225"
     CDS             5986..7248
                     /locus_tag="ACZPDN_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR1297666.1"
                     /translation="MKAKAVRLHGANDLRLDEFELPEIKEDEILVKVVSDSVCMSTYK
                     CAILGVNHKRVHEDVADHPAIMGHEMAGDIVKVGAKHQDKFKPGMKFTLQPALNYKGT
                     MWSPGYSYEFFGGDTTYCIIPAEVMELGCLLEYKGKAYYEASLAEPMSCSIGAFNAAY
                     HTQMGVYKHEMGIKKDGKLAILAGAGPMGLGALTYALHRDIRPKMVVVADVNQERLDR
                     AAHLFPVEEAAADGIELHFVNTGKMEDPAAGLKEITGGTGFDDVFCYAPVEAVVELSS
                     AVLGRDGCLNFFAGPTDKQFSARMNFYDVHYNSTHVMGTTGGNTDDMIESLRLTAENR
                     IDPAVMVTHIGGLNAAAETTLNLPKIPGGKKLIYTHLNMPLVALADLREMGEKDDRYT
                     ALADIVDAHKGLWCPEAEEYLLAHFEQE"
     gene            7307..8068
                     /locus_tag="ACZPDN_03230"
     CDS             7307..8068
                     /locus_tag="ACZPDN_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1297667.1"
                     /translation="MEVMDQRRNEIVEFVNENQTVTFTQLKEKFPSVSEMTLRTDLKY
                     LDQAKRIVRIHGGAKSMDVVSGNDDVLKLRYGRNVNEKKEIAQKALSFVEPAKTIFVD
                     SGSTTTMLAHVLNNQNNIFYTSGLTCAIEMAKLSKSKVFVTGGNLNMQSFSVNGMDGL
                     RCLEKVNFDVAFIGVTRYAKDTGFTCESLEDCELKRKAIERSKKVIVLMDSSKVERKG
                     TYTICDLDQVDVLISDSLLSDSFKQECEKRGLSVY"
     gene            8092..9945
                     /locus_tag="ACZPDN_03235"
     CDS             8092..9945
                     /locus_tag="ACZPDN_03235"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022872 -
                     protein-N(PI)-phosphohistidine-mannitol phosphotransferase
                     system transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HIR1297668.1"
                     /translation="MKTTLQKLGKSLSAMVMPNIGAFIAWGFITALFIADGWLPNEQL
                     ASIQPYMLTYLLPVLIAATGGRMVGGDRGLVMGSIAIIGCIAGVGGTQGQPMLMAAMV
                     MGPFSGWVIKKFDQMMDGHMPAGFEMLINNFSVGILGMLIAILGYYIIGPFMTGVLTV
                     LTYGVDVLVNKGLIPLVAIFIEPAKVLFLNNAINHGIFTPIGAEQAAQTGKSIMYMLE
                     ANPGPGLGVLLAYWLFAKDKATKDSAPGAIIIHFLGGIHEIYFPYILMNPVVIVAPIL
                     GNICAIAFYSIFNIGLKGPSSPGSIIAFLSMAEKGSVFMTALGVLIAAGVSFLVASPI
                     VKLAGEKNLDEASKKMQSMKAESKGRSEALLDVSLVRKIVFACDAGMGSSAMGATKFR
                     NRLKGVRPDLTVINTSVDNIPVDCDVAVVQETLVERAKKSAPFAKVVTISNFLADPAL
                     DNLFFQLSTGDSIMTEAVKEEDEIKPVHEDVIQLDGIKLNLPSVSKEEAITEAGKLLR
                     ELGYVDGAYIPAMLEREELVTTYIGMGLAIPHGTTHGDDVIHKTGIVLLQYPDVIQFG
                     DEKAQLVIGIAGKGGEHMEVLSKICLALEDEAVLNKMKTTNDKEWILKQLS"
     gene            complement(9965..10501)
                     /locus_tag="ACZPDN_03240"
     CDS             complement(9965..10501)
                     /locus_tag="ACZPDN_03240"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR1297669.1"
                     /translation="MNITVYLGSTYGNDPDFKTKIIELGTWIAKNNHTLVFGGSKTGL
                     MGELANSTLNAGGKVIGVEAQMFVDEGVHMDNLTKLYIEKDIADRRTRMIELGDAFIA
                     FPGATGTLEEISEILSKLSLYQLECPCIFYNVNGYYDHLKAFLQHMIDCELSTPKQQQ
                     NIYFLDSIDEIADKLKDE"
     gene            complement(10791..12119)
                     /locus_tag="ACZPDN_03245"
     CDS             complement(10791..12119)
                     /locus_tag="ACZPDN_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family accessory protein
                     TnpB-related protein"
                     /protein_id="HIR1297670.1"
                     /translation="MKVFSMSQRIYYKDLKPDAELIIKKDLELYNCMLHKAFKICFDR
                     AYKDVTYSETDQRMIKSFYGTSDYFPLSAIYEAKALVKSLKCREKEDSDLIKTRIKKI
                     DKKIKKKEKQLKKALKEKEKLINRSKKKKYTEEDYLYEVRVLDPNIKRLKSIIRNLKF
                     RKNRNEFKLKRKMPSVCFGGKKNLKNGDLETFRFRRQRRMLITGRRQGKYSNNLFKLN
                     VDNDMLTYRSTQKDIVFKVQFHKYKEELYARVNEKHNSPDKAVAYELMDYGEYFIVKA
                     IFEKHMNLPKTDTLYGAIGIDINVDHIALCETNMDGNIVLIKKYPIHKENTKNKRNEE
                     LYQLAIEIMEQCKSKKKSLVVEDLNFKQLKTRMLYRPKKQNKTLSSFAYKKILEKLER
                     KCLMNEVDVIKVDPKNTSKIGKEKYTKIKGLSVHYCAAYVINRRGMGFVD"
     gene            complement(12397..13966)
                     /locus_tag="ACZPDN_03250"
                     /pseudo
     CDS             complement(12397..13966)
                     /locus_tag="ACZPDN_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024842.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            14277..14429
                     /locus_tag="ACZPDN_03255"
     CDS             14277..14429
                     /locus_tag="ACZPDN_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297671.1"
                     /translation="MRDGVNLPPRKMMGINSEGMLISAVHEEDGHECLNLLMVDDNIP
                     AGAKLY"
     gene            15093..15416
                     /locus_tag="ACZPDN_03260"
     CDS             15093..15416
                     /locus_tag="ACZPDN_03260"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1297672.1"
                     /translation="MKQLHLKGTEKMLDLACGFGRHSLEFAKRGYDVTGIDITPAYID
                     YANEQAKKENLNAKFICQDIRTITFDKEFDVVLNMADGAIGYFEDDGENHKIFSVIAK
                     ALKNG"
     gene            15426..15752
                     /locus_tag="ACZPDN_03265"
     CDS             15426..15752
                     /locus_tag="ACZPDN_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297673.1"
                     /translation="MDIMNGSYAQTHFPCKLWDAGEKGLTLSAFEWEKDRKTLIYGQV
                     DYMYGEALYKPEMKEGNPIRLYSLDEITEIFCKLGLRICNSFADFSGKPSSDNDIQLM
                     VYSIRE"
     gene            15840..>15979
                     /locus_tag="ACZPDN_03270"
     CDS             15840..>15979
                     /locus_tag="ACZPDN_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1297674.1"
                     /translation="MLQTQNLCKYFRKQKAVNNVSLNIEKGQIYGLLGPNGAGKSTTL
                     KML"
BASE COUNT         5125 a         2482 c         3023 g         5349 t
ORIGIN      
        1 tcataataat agtgtacacc aatacactat taaagtatat aaaaaaataa ataatcttca
       61 aacatgaaaa attgaagatt atttcatgcg taatcactaa aaacagttga aagcgcttgc
      121 aaacatatgt tagaatgcag tagtaaaaag acgcatctca ttgaggagga tacatagctg
      181 actttacaag catagaatag attatataaa ttgattcacg acaaatggct ttgtttcaaa
      241 tacagcaagc aaataacaaa ttaaaaatta ctttttggaa cacccggctg ccaatcatat
      301 tataatcaag cgaagtatct atatgggaaa gtgtcttaat gtaaaaatct acattaggag
      361 gataatatga acaaaagatt tttaacaatt gctgcagctt tatctatggg tgttgcatta
      421 actgcatgta gcggtggagg agatgaagga aaaactggtg gttctagtgt tgcaaatgct
      481 gacaaaccat tagtatggta caaccgtcaa ccttctaaca gttctactgg tgaaatagac
      541 atggatgcat taaacttcaa caaagatact tattatgttg gtttcgatgc aaatcaaggt
      601 gctgaacttc aaggacaaat gattaaagat tatatcgaaa agaatatcga tacattagat
      661 cgtaacggag atggcgttat cggttacgta ttagctattg gtgatgttgg tcacaatgac
      721 tctattgctc gtacaagagg tgttcgtaaa gctttaggta cagctgttga aaaagacggt
      781 gaaatcgtaa gtgatccagc tggtattaat aatgatggta aatctaagag tgttcaagat
      841 ggctcgttag aaattaatgg aaaaacttat acagtaagag aacttgcttc tcaagaaatg
      901 aaaaactctt caggtgctac ttgggatgct gctactgctg gtaacacaat cggtacatgg
      961 actgcatcat ttggtgatga agttgacgtt gtagcttcta acaatgatgg tatgggtatg
     1021 tcaatgttca acgcatggtc aaaagaaaac aaagttccta cattcggata cgatgctaac
     1081 tcagatgcag ttgctgcaat cgctgaagga tatggtggaa caatttctca acatgctgac
     1141 gttcaagctt atttaacatt aagagtatta cgtaatgctt tagatggtgt tgatatcgat
     1201 acaggtatcg gtacagaaga tgatgctgga aacgtattaa gctcagacgt attcacatac
     1261 aataaggatc aacgttctta ttatgcatta aacgttgctg ttactgctga caactataaa
     1321 gatttcttag attcaacagt tacttatgct ccagtttcta accaattaga tgaaaaagat
     1381 cacgctaaga agagtgtttg gttaaatatc tacaacgcat ctgataactt cttaagctca
     1441 acttaccaac cattattaga aaaatatgat gacttattga atttagatgt tgaatatatc
     1501 ggtggtgatg gacaaacaga atctaacatt acaaaccgtt taggaaaccc agacaaatat
     1561 gatgcattcg caattaacat ggttaagact gacaatgcag cttcttacac aggaatcctt
     1621 aaataaataa ttcaataaaa cttaggatta agcttattag ggagaaatta tttctcccta
     1681 atgttattta gatagtagta aacaaaatag gagagatttt atggcagata agaatgaaga
     1741 actagtctta tctatacgcg gaatgagtaa gtcttttgga cgtaaccgtg ttttggatca
     1801 cattaatttg gatttaaaac caggtacagt tatgggactt atgggagaaa atggtgctgg
     1861 taaatcaaca atgatgaaat gtctattcgg gacatatcaa aaagatgaag gtacaatttt
     1921 cttagaagga aaagagatta acttttctgg accgaaagaa gcacttgaaa atggtattgc
     1981 catggtacac caggaattga atcaatgtct tgaaagaaat gttaaagata acttattctt
     2041 gggacgttat cctacaaatg cttttggcgt agtagatgaa gatcgaatga aaaagaaatc
     2101 atctgagctt tttagaaagc ttggtatgac agtgaattta gatacaccaa tgcgtaatat
     2161 gtctgtttca caaagacaga tgtgtgaaat tgccagagca atttcatata attcaaaggt
     2221 tatcgtactt gatgagccta catcttcttt gatgacacaa gaagttaata aattgtttga
     2281 tatgatgcgt atgcttaaaa aacaaggcat ttcgctgatt tatatttcgc ataaaatgga
     2341 tgaaattttc gaaatttgcg atgaagtatc ggttctaaga gatggaaaaa tggttatgac
     2401 aaaacctact tcagaaacca acatgaatga gttgattgca gccatggtag gtcgttcatt
     2461 ggataatcga ttcccacctg ttgataatgt tcctggagaa ccaatgttat ctattcaaca
     2521 tgtatcaaca aaatatgagc catacttaaa agatattaca tttgacgtta aaaaaggtga
     2581 gattttcggt ctatacggtt tagtaggagc tggacgtaca gagttgttag aaacgatttt
     2641 cggtatccgt actagagcct caggtcgtgt ttattttgac aataagttaa tgaactttaa
     2701 tagtgctaaa gaagcaattg aacatggttt tgccctgatt actgaagagc gtaaagcaaa
     2761 tggattgttc ttaaagtgtg atttaacatt caatacaacg attgcaaatt taccttctta
     2821 tatgagcaag gtagcacttt ctgataataa aatggttcaa gctacgaata aagaaatcaa
     2881 aaccatgaga actaagtgta tgggaccaaa tgatgcgatt acaagtctat ctggtggtaa
     2941 ccaacaaaaa gttatcttcg gtaaatggtt agagagatca ccaaacgtat tcatgatgga
     3001 tgaacctaca cgtggtattg acgtaggtgc taaatatgaa atttacgaat tgatcatcaa
     3061 aatggctaaa caaggaaaga ctattattgt agtttcaagt gaaatgccag aaatcttagg
     3121 tatcacaaac cgtattggcg ttatgtcaaa tggacgctta gccggtattg taaatacaaa
     3181 agatactaac caggaagagt tgttaagact cagtgcaaaa tacctataaa ggagataatt
     3241 atgacaaata cagttacaaa tattcttagt cgtgatgaag agcttgcact acgtaaaccg
     3301 attgaggatt atgtaggaaa gatccaagct gaaattgacg aactaagaga agaaggaaca
     3361 aataaagtat tggaatattc tgaagaaatc gaggttctaa agagagataa aatttatact
     3421 aaaagcgaaa aagaagctcg tatttcagaa ttaaatggta aattacaaaa agccaaagaa
     3481 atcgaggcta aaaacaaaca gccaatcaat gacaaaattg cagtagctga aaaatattta
     3541 aaggatcact atcaaacaga ttactacaac aaagttgttg aaagtaataa gtatgaggtt
     3601 caaaaagcta aggcagacta tgataaaaaa ttaaaagaat tagaaaaaga acacaaagat
     3661 attttatcag gtcttagtga taaagaagaa attaaagaag agaactttat ttataagaac
     3721 cgtttgttcg atgcaaaact tgaatatact aaaaatatgc aagaggcaaa agatcgtaaa
     3781 catgatgcgt ttacatttga atatcactta attgacttat tgaagatgtc aaatttctcg
     3841 tttgcagaaa aacaagctca aaaaattgaa aactacaagt actcatttaa tactcgtgat
     3901 tttttattga aaaatggttt gtatattgcg attattttag tattcatttt cttatgtatc
     3961 attacaccaa ttaagaagaa tggtttacaa ctttttactg ttaataacat tttaagtatt
     4021 atgcaacaag catctccaag aatgttcttg gcattaggtg tatctggttt aattatgtta
     4081 gctggtactg acttgtccgt tggacgtatg gttggtatgg gtatgacagc cgctacaatt
     4141 attatgcaca atggacctaa cacgggttca gtattcggac atgtatttga tttctcaact
     4201 atgccagttg gaggaagaat tcttttagca ttagttgttt gtgttgtgtt atgtacaatg
     4261 tttacaacga ttgccggttt ctttgcggct aaatttaaaa tgcacccatt tatttcaaca
     4321 atggcgaaca tgttagttat ctttggtttg gtaacttatt caacaaaagg tgtgtctttt
     4381 ggtggtattg agcaatcaat cccaggcaag attattccta aagttggtgg cttcccaaca
     4441 attatcttgt gggcagtagc tgcagttgtg attgtttggt tcatttggaa taaaacaaaa
     4501 tttggtaaaa acttatatgc cgtaggtgga aacccagaag ctgcttctgt atctggtatt
     4561 tcagtgttta aggttactct tggagccttt attatggcag gaatcttata cggatttggt
     4621 tcttggttag aatgtattcg tatgattggt tctggttcag ctgcgtatgg acaaggatgg
     4681 gaaatggatg caatcgctgc ctgtgtagta ggtggtgtat catttactgg tggtatcggt
     4741 aaaatttctg gcgtagtagt tggtgtattg atctttactg cattaactta ttcattgaca
     4801 attcttggta tcgatactaa cttacagttc gtattctcag gaattatcat tcttatcgcc
     4861 gtaactcttg actgcttgaa atacgtacgt aaaaactaat taattaaaaa aactcttagt
     4921 tttcgctaag agttttcttt atgttttcta catcttcttt agtcgcaaga cctacttgga
     4981 aggcacttgc actaccacaa cacaaaccgt taataaaggc tttttcgtag ttacctgttt
     5041 ctaaatatcc tgcaagaaaa cctgcaacca tagaatcacc agcaccaact gaatttacta
     5101 cggttccttt tggtgcatta ctttgaatta cttcattatt atcacttaca agtacggcac
     5161 cttgtcctgc catagaaatc aatacgtttt gagcacccat ttcctgtagt ttctttgcgt
     5221 aaggaacgac ttcatctcga gtatttaatt cgacattaaa gatttcacct aattcatgat
     5281 tgtttggttt aattaagaaa ggtttatatt ttaatacttt taataatagg ttgtttgtcg
     5341 catcgacaac aacttttatt ttcttttcct gaactcgttc tataatctgt tcgtacattg
     5401 tatcaggcaa agtgcttgga atacttcctg aaataacgag aacatctttt tctgttaatt
     5461 catctaattg attgaataat tcttgaatat cttccggttg aatatcaggt cccataccat
     5521 tgatctctgt ttcttcatcc gatttcatct taacattgat tctagataat ccttttttta
     5581 catgaatgaa atcggtttta gctccatagc tttctaattt atgctcaatt tcttttcctg
     5641 taaaacctgc cataaaacca agggctgtac tttcgtgtcc gagattggat aggacaattg
     5701 aaacgttaat gccttttcct ccgggaagga tttcttcgaa ttttgtttta ttaatgatac
     5761 caggcttaaa atgatcgact tgaatgacgt agtctaaaga ggggttaaaa gtaattgtat
     5821 aaatcataat gatctccttt tctttatctg attataggat actcctttta ttgttgtttc
     5881 gcaagtgaaa tatttttgaa ataacagttg acaattaaaa taattggttt attatgtaga
     5941 tgtagctttt gaaacaacaa tttacagaga gaggagactt ttgaaatgaa agcaaaggca
     6001 gtgcgattac atggagctaa tgatttaaga ttagacgaat ttgaattacc agaaattaag
     6061 gaagatgaga ttttagtaaa ggttgtctca gacagtgtct gtatgtcaac ttacaaatgt
     6121 gccatcttag gagtgaatca taaacgtgtt catgaagatg ttgcggatca tccagctatt
     6181 atgggacatg aaatggcggg tgatattgtt aaggttggtg ctaagcatca agacaaattt
     6241 aaaccgggaa tgaagtttac tttacaacca gcattaaact ataaaggaac aatgtggtca
     6301 cctggttatt cttatgaatt ttttggtgga gatacaactt actgcattat tccagctgaa
     6361 gtaatggagc taggatgctt actggaatat aaaggtaaag cttattatga agcaagttta
     6421 gctgaaccaa tgtcatgttc gattggagcg tttaatgcag cttatcatac acagatgggt
     6481 gtgtataaac atgaaatggg aattaagaaa gatggaaaac ttgcgattct tgcaggagca
     6541 ggtcctatgg gacttggtgc actaacatat gcgttgcata gagatattcg ccctaaaatg
     6601 gtagtagttg cggatgtaaa tcaagaaaga ttagatcgag cagctcattt attccctgtt
     6661 gaagaagcgg cagcagatgg aattgaattg catttcgtga atacaggaaa gatggaagat
     6721 cctgcagccg gcttaaaaga aattacaggt ggaactggtt ttgatgatgt attctgttat
     6781 gcaccggttg aagcggttgt ggaattgtca agcgctgtat taggtcgtga tggatgttta
     6841 aatttctttg cgggacctac ggacaaacag tttagtgcaa gaatgaattt ctatgatgtt
     6901 cactataatt cgacacatgt aatgggaact acaggtggaa atacagatga tatgattgaa
     6961 tctttacgct taacagcaga aaaccgtatt gatccagctg ttatggttac gcatattggt
     7021 ggtttgaatg cagcggccga aacaacatta aatttgccta agattccagg cggaaagaag
     7081 ttgatttata cgcatttaaa tatgccattg gttgcattgg cagatttaag agaaatggga
     7141 gaaaaagatg atcgatatac tgcgttggca gatatcgtag atgctcataa gggattgtgg
     7201 tgtcctgaag ccgaagaata tttattggct cattttgaac aagaatagat tttattaaac
     7261 caagaatctt gtgtatacta aatacagtaa atacaggagg tattgtatgg aagtaatgga
     7321 tcaaagaaga aacgaaattg tagagtttgt gaatgaaaat caaacggtaa cttttacaca
     7381 attaaaagaa aaatttcctt ctgtgtctga aatgacattg cgtacagatt taaaatattt
     7441 agatcaagct aaaagaattg ttcgaattca tgggggagcc aaatctatgg atgtggtttc
     7501 tggaaatgat gatgtattaa aacttcgtta cggacggaat gtaaacgaaa agaaagagat
     7561 agcgcaaaag gcattatcct ttgtagaacc agctaagaca atttttgtgg attcaggcag
     7621 tacgactaca atgttggcac atgttttaaa taatcaaaat aatatttttt atacaagtgg
     7681 cttaacgtgt gctatagaga tggcaaaact ttctaaatcg aaagtgtttg tgacaggtgg
     7741 aaatctaaat atgcaaagtt ttagtgtgaa tggaatggat ggacttcgct gtttagagaa
     7801 ggtcaacttt gatgttgcgt ttattggtgt aactcgttat gcgaaagata cggggtttac
     7861 atgcgaatct ttagaggatt gtgagttaaa gcgaaaggca attgaaagat ctaaaaaagt
     7921 aattgtgtta atggattcta gtaaagtgga aagaaagggt acatatacaa tttgtgattt
     7981 agatcaagtg gacgtgttga tcagtgattc gttactttca gatagtttta aacaagaatg
     8041 tgaaaaacga ggactatctg tatattaaag aagtcctaag aaaggacaat tatgaaaaca
     8101 actcttcaaa agcttggtaa aagcttatct gcgatggtta tgcctaatat tggtgctttt
     8161 atcgcttggg gatttataac agctttattt attgcggacg gatggttacc aaatgaacaa
     8221 ttggcatcga ttcaaccata tatgttgact tatttattac cggtattaat tgcggcaacc
     8281 ggaggaagaa tggttggcgg tgatcgtgga cttgtgatgg gctcgattgc gattattgga
     8341 tgtattgccg gcgttggtgg aacccaagga caacctatgt tgatggcagc catggttatg
     8401 ggaccatttt ctggctgggt aattaaaaaa tttgatcaaa tgatggatgg acatatgcct
     8461 gctggctttg aaatgttgat caataatttc tctgtcggta ttttaggtat gttgattgcg
     8521 atattaggat actatattat tggccctttt atgacaggtg ttttgactgt gttaacatat
     8581 ggagttgatg tacttgtaaa taagggatta attcctttgg tagcaatctt tattgaaccg
     8641 gctaaggttt tattcttaaa caatgcgatc aaccatggaa tatttacacc aattggagct
     8701 gaacaagctg cacaaacagg caaatccata atgtatatgt tggaggctaa tccaggtcct
     8761 ggtttaggtg tactattagc ttattggtta tttgcgaaag ataaagcaac gaaagattct
     8821 gcgcctggag ctattattat ccatttctta ggtggaattc atgaaattta ttttccatat
     8881 attttaatga atccagttgt gatcgttgct ccaattttag ggaatatttg tgcaattgca
     8941 ttttactcta ttttcaatat cggattgaaa ggtccatcaa gtccaggttc aattattgcc
     9001 ttcttgtcta tggcagaaaa aggctctgtg ttcatgacag cattaggtgt tttgattgcg
     9061 gctggcgttt cattcttggt ggcaagccca attgttaagc ttgctggaga aaagaatttg
     9121 gatgaagcta gtaaaaagat gcagtctatg aaagctgaaa gtaaaggaag aagtgaagct
     9181 ttattagatg tttctttggt tcgtaagatt gtattcgcgt gtgatgcagg tatgggatct
     9241 agtgcaatgg gagcgactaa atttagaaac cgtttaaaag gtgtgcgacc agacttaact
     9301 gtaattaata cttctgtgga taatattcca gtggattgtg atgttgcggt cgtacaagaa
     9361 actttggtag agcgtgcaaa aaaatctgca ccttttgcga aagtagtcac aatttcaaac
     9421 ttcttagccg atccagcttt agataatttg ttcttccagt tgtcgacagg agattcgatt
     9481 atgacagaag cagttaagga agaggacgaa ataaaaccag ttcacgaaga tgtgattcaa
     9541 ttggatggaa ttaaattaaa ccttccttct gtgtccaaag aagaagcgat tacagaagca
     9601 ggaaagttat taagagaatt aggctatgtg gatggcgctt atataccggc tatgttggaa
     9661 cgtgaagaat tagtaactac ttatattggt atgggattag ctattcctca tggcactaca
     9721 catggtgatg atgtgattca taaaacaggt atcgtattgc ttcaatatcc agatgtaatt
     9781 cagtttgggg atgaaaaagc acagcttgtg attggtattg caggtaaggg tggagaacat
     9841 atggaagtgt tatctaaaat atgtttggcc cttgaagatg aggctgtact caataaaatg
     9901 aagacaacaa atgataaaga gtggatttta aaacaattat catgataaag aaggacgaga
     9961 aaaattactc gtcctttaat ttgtctgcaa tttcatcgat tgaatctaag aaatagatgt
    10021 tttgttgctg ctttggtgtg gataattcac aatcaatcat gtgctgtagg aatgctttta
    10081 aatgatcata atatccattt acattataga aaatacatgg gcattcaagt tggtacagtg
    10141 aaagttttga taagatttca ctgatctctt ctaatgttcc agttgcacca ggaaaggcaa
    10201 tgaaagcatc accaagttca atcattcttg ttcttcgatc ggcaatatct ttttcaatgt
    10261 ataacttggt aagattatcc atgtggacac cttcatccac aaacatttgt gcttctacac
    10321 caattacttt gccacctgca tttaatgtgc tattggctag ttctcccatt aatccggttt
    10381 ttgatccacc aaatactaat gtatgattgt ttttggcaat ccatgtacct aattctatta
    10441 tttttgtttt aaagtctgga tcatttccat aggttgatcc taaatatact gttatgttca
    10501 taaactacct cctgtattta gtttacatta aaattgtgca caagaaagac tttttctacg
    10561 aaaaagtcac acgtctaaat ggcgtgcccg gttcgtttcc agtcgtccgg taacatttca
    10621 atatccactc catctttctg aatcattcgg tatcccgatt cattcagtct tttgcgatgg
    10681 agtttttatt gtatgcttta tttataaaca cttcagaatt tcatccatga tctatgtacc
    10741 tatcatccag taagatatca taaacttctg gttctgatta tttatctcat ttaatccaca
    10801 aatcccatgc ctcttcgatt gatgacatag gctgcacaat aatgtacact caatcctttg
    10861 atctttgtat acttttcttt tccaatctta ctcgtattct ttggatctac tttgattaca
    10921 tccacttcat tcatcaaaca ttttctttct agtttttcta atatcttttt atacgcaaaa
    10981 ctcgataatg ttttattttg ttttttaggt cgatagagca ttcttgtttt caactgcttg
    11041 aaattcaagt cttcaactac aagacttttc ttcttggatt tacattgttc catgatttca
    11101 atggccagct gatacagttc ttcatttcgt ttgttctttg tattctcttt atgaattgga
    11161 tacttcttga tcaatacgat atttccatcc atattggttt cacatagtgc tatatggtcc
    11221 acattgatat cgattcctat tgctccatac aacgtatcag tctttggaag attcatatgt
    11281 ttttcaaata tagctttcac aataaagtat tcaccataat ccatcaactc ataggctact
    11341 gccttatcag gtgagttgtg cttttcattg actcttgcat acagttcttc cttgtattta
    11401 tgaaactgca ctttgaatac gatatccttt tgtgtgcttc gatatgtcag catatcatta
    11461 tcaacattta atttgaaaag gttgtttgaa tacttgccct gtcttctgcc tgtaataagc
    11521 attcttcttt gtcttctaaa tctaaaagtt tctaaatcac catttttaag attcttttta
    11581 ccaccaaaac atacagaagg catctttctt ttaagcttga actcatttcg attttttctg
    11641 aatttaagat tgcgaataat actcttcaat cttttgatgt ttggatccaa cacccgcacc
    11701 tcatacaagt aatcttcttc tgtatatttc tttttcttgg atctgttgat cagtttctct
    11761 ttttccttta aagccttctt taattgcttc tccttctttt tgattttttt gtctatcttt
    11821 ttgatacggg tcttgatcaa atcactatct tccttttctc tacatttaag actcttcacc
    11881 aaagccttgg cctcatatat ggcagacaaa ggaaaataat cacttgtacc atagaaggac
    11941 ttgatcattc tttgatctgt ttctgaataa gtaacatcct tataggcacg atcaaaacag
    12001 attttgaatg ccttatgaag catacaatta tacagttcaa gatccttttt aatgatcaat
    12061 tctgcatctg gtttcaaatc tttataatag attctttgcg acattgaaaa caccttcata
    12121 agacaagtcc tcctgttcta tctgactcta ttatactata ttcaagacag acttaataga
    12181 tagatttgag actttctaat ttattttgtt tgtatcatta tatcaccaat gtgaatatgt
    12241 gatatttgtt gatattctca catgttcaca ttaaattcac attgacattg cattttattc
    12301 aattcagaat aatttcattc tatactacat ttattatcat tcaaacattc cagttaaaac
    12361 cttattacta ttagattatg atattttctt gttttatcac atatgatatt ttcctgaaac
    12421 cataatctta atatacaaaa aaagctcaga ttgttgacaa aagcctatac cttttcgtaa
    12481 ggacataggc tttttattgt atataggcaa ttttcgaaat gaaattgtct ttttttgtat
    12541 attttgaagg atatattgaa ttttttgatg gctttcttct atattttcct ctccaaaggg
    12601 acattttctt cagattatgg cacgcaaaaa tcaaccaagc attctgttgg tttttcttca
    12661 agcctttcaa ccttgtgaaa cgtagtccat tgttttcttt acagtccgca aatactcgtt
    12721 caatggtttc ttttctctgt ggataaattt ctttccattc catagtatga cggatctcat
    12781 cggcatattc tctaaactct tcccataggt gacgttcgat aacttttgtc atgttcttac
    12841 tctttgtaca tttgtttcta agaggacaat ccttgcagtc ttttggatcc gatttatatt
    12901 gtctgtatcc atttcttgta gtagttgtat acattaggat cttatcattt ggacatagat
    12961 aacagtcgta ttcttcatca tagacatatt cgtatttttt aaagaagcct tttttggtca
    13021 ttggtctttt atatggcata taaggaatct gatcattttg tacaatggct ctgcatattg
    13081 cgggtgtcat gtatccggca tctaacgaaa cattcttaat caggtatcca tattttccat
    13141 tgatcaattc ttcataggca tcaaaaaaac taacactatc atgtacattt ccaggaactg
    13201 ttgtaactgc cagtacgaac gaattcttat cgcagaaagt ttgatgcgag tacgcaaaac
    13261 atttctcctt ctcaccttta tggaagcagc cactttctgg atctatagtg ctttctgtaa
    13321 ttgttttagt tgaagttttt tttacttctt cacccgtttc ttcatcatgt acatattctg
    13381 tatgtaccat tgaatcaaag gcctctttac ctatacattc tctatcctcg ttgatttcaa
    13441 ccaacagatc atcttcgtat ttctttttga ggatctctac ttcttttttg acactttttc
    13501 ttttattgac gtttgccttc tgatgcgtac tgtctccaaa taccgttgtg aaatcgacaa
    13561 agccataatc aacggcctgt tctaatattt ccataaagat tttttggaat atagttgaat
    13621 ctttatatct acgaatatag ttctgagacc atgtagaaaa gtttggaatc ttctcatcaa
    13681 tacttaatcc aagaaaccag cgatatgcca agttgacttc gatctcctga catgtcttgc
    13741 gcatagaatt gattccaaac acgatattta tgataagcat tttaaaaaga accacaggat
    13801 cgatactcgg tctgccgtga cgactgtaaa gatgctgaac tttcggataa ataaaccgaa
    13861 aatcaagagc atcctccatc ttacgaacca aatgatcctg tggtactaat tcatctaatg
    13921 tatagtaaat catgcaatct aattttgcat tgtttctgtt agtcatagcc attgtgatat
    13981 cctccataaa ccctctataa ttatatcatt tttggcataa taaatggcag aaacgtcgac
    14041 tatttttggg tttatgaagt acgtttctgc cacagatatt ctatcagatt ttaaaaagac
    14101 tgtcgacagt cactttgtca acagtctgaa ggaatcaact gtatagttga ttcctttttt
    14161 ttattcggct tttattgttt aatgacgggt tcttttattt gcttcatcaa agtatttata
    14221 aatgacagcg caacaataat ttttatgatt ttgaagatga tttataaatt tgaaacattc
    14281 gcgatggtgt aaacttacca ccaagaaaga tgatgggtat taattctgaa ggtatgttga
    14341 tttcagctgt tcatgaagaa gatggtcatg aatgcttgaa cttattgatg gttgatgaca
    14401 atattcctgc aggagctaag ctttactagg ataaaaaaat tagtaatttt tggttataaa
    14461 tttacgcatt tacgccaaac ttacgcccaa cttacgccaa aaaattaaga agaaaaagat
    14521 ggttacacta gtaaagtgaa gcattttgta ttgaattaaa caaagagcaa ctctctgaaa
    14581 agtgagttgc tcatttttgt tattaaattt tgagaaaaat aatatagcaa ctaatttaat
    14641 acaacacaga taatatgaca ccctttttaa tgttgattgg ttactgatat cattgaactt
    14701 tcaacgtttg attttaaatg acaccttttt taatgtgtct ttcaaaatat aagtgaaaaa
    14761 tacgctatac atgtgttttg aatatagtaa ttgtgtgtta ttgaggttaa acgcagtaaa
    14821 atataacata ttggtattat aaaagttgct gtttccaaaa aatagcgtcg aaaaatagac
    14881 ggttgtcatt aaaaaaagtt gtcacgttga cactaatgac attaaaaaag ttgcgatatc
    14941 gataaaaagg cagcttaact agcaattagt attataaaag ttgtcattat gcctaaatag
    15001 acattaataa agttgctata ttcgctataa atagttctaa atgcgattgg ttatcatggg
    15061 aagaagacac caacagacta gtagattttc taatcaagca gttacattta aaaggaacgg
    15121 aaaaaatgct tgatcttgca tgtggatttg gtcggcactc actggagttt gccaaacgtg
    15181 ggtatgatgt taccggtatt gatattacac cggcgtacat agattatgca aacgagcagg
    15241 caaaaaaaga aaatttgaat gcaaagttca tttgtcagga cattcgcacg atcacatttg
    15301 ataaggaatt tgatgttgtg ctgaatatgg cggatggagc aataggatat tttgaagacg
    15361 atggggaaaa tcataagatt ttttctgtca ttgcgaaagc attaaaaaat ggttgaaagc
    15421 actttatgga tatcatgaat ggaagctatg cccaaacaca tttcccttgc aagttatggg
    15481 atgcaggaga aaagggactt acattatctg cttttgaatg ggagaaggat agaaaaacgc
    15541 tgatatatgg gcaggtagac tatatgtatg gagaagcact ttataagcct gaaatgaaag
    15601 aaggaaatcc tataaggtta tatagtttgg atgaaatcac ggagatattc tgtaaattag
    15661 gacttcgtat ttgtaacagc tttgctgatt tcagtggaaa gccgagttct gataatgata
    15721 ttcaattgat ggtttattcc atacgggaat aaatcttcat caaatcttta tttttttctg
    15781 ttaggttata tttcagtaag aaatgaaata tagaaggaag gatagtaaag atggaaatga
    15841 tgttacagac acaaaatcta tgtaaatatt ttagaaaaca gaaagcggta aataatgttt
    15901 cacttaatat cgaaaaggga cagatttatg gactgcttgg accgaatggc gctggtaaat
    15961 ctaccacatt gaaaatgct
//
LOCUS       DBMDWL010000028        15850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1250_length_15850_cov_2.092196, whole genome shotgun sequence.
ACCESSION   DBMDWL010000028 DBMDWL010000000
VERSION     DBMDWL010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 15850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15850
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1250_length_15850_cov_2.092196"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="ACZPDN_03275"
     CDS             <1..238
                     /locus_tag="ACZPDN_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016177.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="motility associated factor glycosyltransferase
                     family protein"
                     /protein_id="HIR1297675.1"
                     /translation="QKISVMNVGEYEIDNTYRHISFYPILEGMLSLSGFLYPLQSRRI
                     HIEEIYTTSNSLVEDKGIVRIEEGRFLCVQSNWR"
     gene            242..1510
                     /locus_tag="ACZPDN_03280"
     CDS             242..1510
                     /locus_tag="ACZPDN_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HIR1297676.1"
                     /translation="MFYDVAMNFVIGTILMVAGAIMLCIPNRVFTWILVVSILFLFAN
                     GVTLFIRFIKNKKNKDFFFSILSFAFMFFLMNFRYIPQWILRVNFGGYCILCGSACFI
                     QLVIDKINHFKGKFFNFVFTLIYILFGFYLLLNPNFHTDLLMQAFGIYFMILGLRYLG
                     DGYVGINPLTKYKWKRKVRITLPAFLCALVPDAALASINRYLEEGKPEDLNTYKMEDP
                     VRLKVIVHVGPKGFQKVGHICFSYDNIVYSYGNYDSDSFRLNQTIGDGIFFTVPLEKY
                     IPNMISAENNSIFEYGILTTPTQNAFIEKEIEKIRLNGYRWYTKIEKEDGYDHFSQYE
                     MDYPSRLHYRTGAKLYKVKSGKFHIYWALGDNCASFTDLVLGTLGADVLSIRGIISPG
                     TYLDWLQKEYLKKNSPIVSRCIYTKETVEQ"
     gene            1511..2590
                     /locus_tag="ACZPDN_03285"
     CDS             1511..2590
                     /locus_tag="ACZPDN_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1297677.1"
                     /translation="MNGYFYVLTTIDIFVLCFMCVLTRLSESLNKKQKRGFFFAFILI
                     AFISVLEVVTLKVDGLPVRYRWINIISNYLGFGLTPSVCICLVYVFDKKTRLRWEFKL
                     AILCEIIYLLVLAISIPSGLVFSVSQENIYSRGPYFFIYIFAYFISIFYLSLSTILVS
                     KQFQNRSKALIYPLIIFLAAETIIQILLPNLHVSWLCVTLLSVLYFIYCSEMWNQLDS
                     LTGLLNQNSFLNRSHEMNYTNGMLIVFDVNDFKHVNDVYGHVKGDLCLSIIANCIKKA
                     YAKYGYCYRIGGDEFCVLLKNCRNEESCSIQFDYLMKSKQRKYKYLPSVSYGSALLLT
                     GDIVSVKDLADQNMYLNKKRNKEQQ"
     gene            complement(2610..2795)
                     /locus_tag="ACZPDN_03290"
     CDS             complement(2610..2795)
                     /locus_tag="ACZPDN_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297678.1"
                     /translation="MNKQDKDELLNQGLTQKEIEDLTQKGFSKEEIIQSHQYDNEIKK
                     GSTFIAWIVDLITWFLP"
     gene            complement(2808..3644)
                     /locus_tag="ACZPDN_03295"
     CDS             complement(2808..3644)
                     /locus_tag="ACZPDN_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1297679.1"
                     /translation="MSTQRFQIDQVDFNKARPKLLYITESKFDHEWNSTLHTHLYTEI
                     FYVTRGHGLLSLDNKNYPVKEDDLVIVNPLVSHTERGIENVNFEYIVMGIEGVTYFSK
                     EEDEEQGCSIANYCEYKHEVLFYLKTILFEVKTKDTEYQSLCQNLLEVLLINLMRRAN
                     INLKSSPINKANKDCVLIEKYIDAHFKEDITLDILSELTFLNKYHIVHAFKQYKGISP
                     INYMIQKRLEEAKLLLSTTNLQISEIAYIVGFTSQSYFAQAFKKATHTSPLQYRKDNS
                     HS"
     gene            3827..5155
                     /locus_tag="ACZPDN_03300"
     CDS             3827..5155
                     /locus_tag="ACZPDN_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1297680.1"
                     /translation="MKKVITAGLSFAMAASMLAGCSSKPAENSDSGDKRVLKFDSFSG
                     GNGEKVFTKLAKAFEKANPDVDVQLRFEKELPDVLNKENAKGEYSDVVYYNLGQPSGY
                     TETQLNTHEVLDISDVVKEVGMDENYANSSIVQYYGDGKSYLMPLKTTPAGFFYNTEL
                     VGEGKKYDLPTTWDEFWALGDQAKQDGISLFTYPTNGYFDNTLNALLKETGGEELLSN
                     VLNYDKDAWNTDGAKQTIDILTKLVSPDYLYSDTVSNANAKDGFTMNQQAVIDGKALF
                     MPNGDWVVNEMADTTPEDYHWGLLPLPALEKDGERFVGSMTEQVWIPAQAKNVDDAKA
                     FLKFIYSEEGVKIMAEGGNIVPTDTMSETIAAMEDGYTKEFFSVYDNASAALGAFAPY
                     NTDNLPDFDRAATVFGSIDSLATGELSAKDYQTKLSDAWAKLAKNKAVAE"
     gene            5231..>5889
                     /locus_tag="ACZPDN_03305"
     CDS             5231..>5889
                     /locus_tag="ACZPDN_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1297681.1"
                     /translation="MNKKKAQNRFVFACLAPAVILVVIFMVIPTIDVFKTAFFKKSNF
                     VSDATFAGLFNFKVLFNDMRFIEAMQNTILYVVLITIITLVLAVLFATLLTREEFVGK
                     NFFRVIFYIPNILSIVVIAGIFSAIYQPTNGILNSFLGMIGLESLQRQWMGNPAIINY
                     SIIGALVWQAIGYYMVMYMSSMTSIPENLYEAASLEGASKVKQFFMITLPLIWDNIRT
                     T"
     assembly_gap    5890..5899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5900..6114
                     /locus_tag="ACZPDN_03310"
     CDS             <5900..6114
                     /locus_tag="ACZPDN_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR1297682.1"
                     /translation="VISTINLSFMFVQLTSEGNLGSEVALNYMYNNAFAGKYGFGMAI
                     GVCIFVFSFALSAIINKVTERDVLQY"
     gene            6126..6986
                     /locus_tag="ACZPDN_03315"
     CDS             6126..6986
                     /locus_tag="ACZPDN_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1297683.1"
                     /translation="MKKRNFPLYKIFVYVALITLAISIIVPIAWVFLASLKSQLELVS
                     GNPFDFPKQMLWSNFADAFVKARMGDYLLNTIIVTALSLVILLVVALPASYVLARFDF
                     VGKKFFNGLFMAGLFVNVNYIVLPIFLMLFNADIKLGVDFFLNSKFWLSVVYAATALP
                     FTIYLLTGYFKTLPKAYEEAARIDGCGYFKTMTKIMIPMARPSIITVILFQFLAFWNE
                     YIIAFTFMDKDNATLAVGVKYLMADAKSQANFGVMYAGLVIVMIPTLILYICVQKKIT
                     QGMSIGGMKE"
     gene            6999..7172
                     /locus_tag="ACZPDN_03320"
     CDS             6999..7172
                     /locus_tag="ACZPDN_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6903 family protein"
                     /protein_id="HIR1297684.1"
                     /translation="MRDDNKVMNIVMIILFFVFLGLIIWAQKTVSVRNLMIEIVGLTG
                     LLTLLFLYNKKHK"
     gene            7184..8275
                     /locus_tag="ACZPDN_03325"
     CDS             7184..8275
                     /locus_tag="ACZPDN_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297685.1"
                     /translation="MAELSLQHVDKIYDNNVQAVFDFNLDVKDKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISAGDFYIDGKRMNDVEPKDRDIAMVFQSYALYPHMSVYENMAFGLK
                     LRKFSKEEIDKRVHEAARILEIEPYLDRKPKALSGGQRQRVALGRAIVRNAKVFLMDE
                     PLSNLDAKLRVQMRSEIIQLHERIGATTIYVTHDQTEAMTMADRIVVMKGGYIQQIGT
                     PKEIYNNPANLFVAGFLGAPATNFIKGVYKNGFFYIDDMKIDIPLMYREKLSSYEEKE
                     IIMGIRPEDLHGEGIVADTYPSANFDFEIEVAELLGHEYILHGSLNGQKMCAKVNSRL
                     EPEAHTTIKLTMDLSKVHFFDLETENRID"
     gene            8293..10437
                     /gene="gnpA"
                     /locus_tag="ACZPDN_03330"
     CDS             8293..10437
                     /gene="gnpA"
                     /locus_tag="ACZPDN_03330"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HIR1297686.1"
                     /translation="MIETGRVTVPTDVDVIDATIEIIHRWKADAIRDCDGTDMPEELR
                     KMDIKQYATYYTTRKDNAWAKANPDEIQQMYLMSDFVTAKDTELKIQIMQHFYSVQLK
                     PNTKDNHRWWEVIDRTTDEVVTNWDYDEKTMEVIIHDTIPYHAYTVSFLAFVIWDPVH
                     MYNALTNDWKDEEHQMTYDVRQPKTQKYVLDKFRKFCEERDDVDVVRFTTFFHQFTLQ
                     FDEFAREKFVDWFGYSASVSPYILEQFEKEVGYPFRAEYIINKGFNNSTFCVPTKEYR
                     DFMDFQQREVSKLAKKFVDICHEYGKEAMMFLGDHWIGTEPFGKYFESIGLDAVVGSV
                     GSGTTLRLISDIKGVKYTEGRFLPYFFPDVFHENGDPLKEAKVNWVTARRAILRSPVD
                     RIGYGGYLKLALDFPEFIDYIEKVCDEFRTLYSHVNQQVPHNLLKVGVINSWGELRRW
                     GTHLVHHAIWYKQIYSYTGVMEALSGFPFDVRFISFDDIRKDPSILEDMDVVMNIGSA
                     YTSFSGGAEFDETIITALRRYVDQGGGFIGVGEPTAINRNGKYFTLYDVLGVDKELGF
                     TMHTDKYNWEVHPNHFITEQLEHEDWGECINNVYALPGTEILAEKDLHVNLAVNEYGK
                     GRGVYMNGLPFSFENVRLLYRAIFFAAHKEDEMKHWYSENYNVDVNVYPSTNSFCVVN
                     NTYEPQTTTIYKGDGSSFEVELAECEIQWFEI"
     gene            10541..10891
                     /locus_tag="ACZPDN_03335"
     CDS             10541..10891
                     /locus_tag="ACZPDN_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297687.1"
                     /translation="MDSLIFDEKKKEFTVLDGSLGKYSFYDVVSSQIVYEDAKYKDKS
                     PMFSHKVLVSTLNLSIFIEMKKIYVGIEIQFLNGNKVYVYISKSPVIQHNFQFKQDLK
                     IAKCLNEKLKEFYK"
     gene            complement(10963..11154)
                     /gene="rpmB"
                     /locus_tag="ACZPDN_03340"
     CDS             complement(10963..11154)
                     /gene="rpmB"
                     /locus_tag="ACZPDN_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR1297688.1"
                     /translation="MSRVCAVSGKGPLSGNKRSHSMRASRRKWNVNLQKVRVVVDGKP
                     QTIRVSARTLKTLKKQNAI"
     gene            11301..12281
                     /gene="mraY"
                     /locus_tag="ACZPDN_03345"
     CDS             11301..12281
                     /gene="mraY"
                     /locus_tag="ACZPDN_03345"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1297689.1"
                     /translation="MLNSNYIWIIGMCLITLVIMLVIMPGLIDYLHKIKFGQTEREEG
                     LASHKKKNGTPTMGGLAFIIVPTLVYVIGSFFTPFKLDMNTMIILLAFVGYGVVGFID
                     DYIIVVQKNNEGLKPAHKFLLQSVLAVVFFFLYRTNSTTDIWIPIFDVTINLSWFYFI
                     LVFLMFTAETNAVNLTDGLDGLCAGQMVIALVPFALFAFIQGLNNVACLICMVIFALL
                     GYLKFNKHPAKIFMGDTGSLALGGFIAAIAMVLKQEILLVVIGFVFVAEVLSVIIQVT
                     YYKKTHKRIFKMAPLHHHFEMCGWSEEKVVTRFRLVGVILAILGLVLGVM"
     gene            12283..13605
                     /gene="murD"
                     /locus_tag="ACZPDN_03350"
     CDS             12283..13605
                     /gene="murD"
                     /locus_tag="ACZPDN_03350"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1297690.1"
                     /translation="METVLVIGAARSGIAVSKLLLKNGYHVILTDSNKVNEKSELESL
                     GIEVYDEGHPDCLKEKKYAFIVKNPGIPYRVPFVHYFVEQNAKIYTEIEVAYRYAKNF
                     DYAAVTGTDGKTTVTTLLFEMLNRQKKSLVAGNIGTPLCELALEYTDTNYNVALELSN
                     FQLLGIETFRPHVSTVTNLAPDHLDYMDSLEAYYESKMRIYENTNADDYFIRNVDDEN
                     VVKYAQNIPCRVIDFSLKRKDVDLYKDDQYAYYKDTQLFKLSDLKIVGEFNCGNAMMA
                     SCMAYLMGVSLFNIQEVIREFSGVEHRIEYVDTIDNVRFYNDSKATNTHAACAGLSAF
                     EKNVILLCGGKDKHISFDDLKQYDAVVKKCISFGQTKDKFKDIFSNQVSVETMKDAFE
                     KAVLFAKPGDIVLLCPACSSFDQFKNYEIRGEIFKEMVHDYASKRNEE"
     gene            13602..14417
                     /locus_tag="ACZPDN_03355"
     CDS             13602..14417
                     /locus_tag="ACZPDN_03355"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1297691.1"
                     /translation="MIDIIKTIFLGILQGITEWLPISSTGHLILFSSIWPLEPAKFFE
                     VFKVVIQFGSILAVLVLYEQKLNPFDSRKSAKKKKQTLQLWSKVIVGVIPASIIGLLL
                     DNIVEDYLSKNIVIAITLILYGMIFIFVEKHPKKEKVQTIERLDYKSALKIGCFQCLA
                     LIPGTSRSGATILGGLYCGCSRTVASEFSFFLAIPVMFGASLLKVIKYLMKVGLFSFG
                     QLFLLLLGTFVAFVVSLFAIKALLNYVKNHDFTVFGWYRIILGIFVILFFYIL"
     gene            14427..15320
                     /locus_tag="ACZPDN_03360"
     CDS             14427..15320
                     /locus_tag="ACZPDN_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1297692.1"
                     /translation="MNRFDLHMHSCYSSDGQFTPKELIEIAKDKGLKTIALSDHDCIS
                     GVKEMIRLGNEVGIEVIPAIECSTSIGYTDVHLLGYGIDLDNDYFQNLLEKTQVKSRN
                     AFSTRCDRLRAKYGVEIDEEAILKEANGKNPWFVMCTHMFNDPRYQDIPDFKDYIPGG
                     KRSDPAPVNFFWDKCQYGSDLFVYVPMPDFVESVQKIHEAGGLAIVAHPFTTFYKNDE
                     MLKILLDSGVDGIEAYSNYHTPEQNVYYENLAKEHHLLITCGSDFHGEKKPSIEMGEY
                     GLEKDGSVWLERIKEKIKAAR"
     gene            complement(15330..>15850)
                     /locus_tag="ACZPDN_03365"
     CDS             complement(15330..>15850)
                     /locus_tag="ACZPDN_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1297693.1"
                     /translation="LDTNIAYDYFETISDLIDTKEVQDLKKCSQHLATSRFQHSLNVS
                     YYSFLICRKFGLDAYSAARAGLLHDLYYYDWKTNDKRPLEGNHAKIHPIIALENAKNI
                     TVTNPTIDDAILHHMWPLKTSHPNTSVGWIIQAVDKFCAITEMGHQSLFRASRSNNLL
                     SYCILFTFLLTM"
BASE COUNT         4980 a         2066 c         3205 g         5589 t
ORIGIN      
        1 tcaaaagata tctgttatga atgtagggga gtatgaaatt gataatacgt atcggcatat
       61 ttcattctat ccaattttag aaggtatgct ttctttatct ggatttttat atccactaca
      121 atctagacgt attcatattg aggaaatata tacaacaagt aattctttgg ttgaagataa
      181 aggcattgtt cgtatagaag aggggcgctt tctttgtgta caatccaatt ggaggtagtc
      241 tatgttttat gatgtagcta tgaactttgt gattggaacc attttaatgg tggctggagc
      301 tatcatgctg tgtattccaa atcgcgtttt cacatggatc ttagttgttt ccattctgtt
      361 tttatttgcg aacggagtta ctttatttat tcgttttatt aaaaataaaa agaacaaaga
      421 ctttttcttt tcaatcttat cgtttgcgtt tatgtttttc ttgatgaact ttcgttatat
      481 tccgcaatgg atcttgcgag tgaattttgg tgggtattgt attctttgtg gttctgcttg
      541 ttttattcaa cttgtgattg ataaaatcaa tcattttaaa gggaagtttt ttaactttgt
      601 gtttacactt atttatattt tatttggctt ttatcttctt ttgaatccga attttcatac
      661 ggatctttta atgcaagcat ttggaatcta ttttatgatt ctaggcttac gttatttagg
      721 cgatggatat gtgggaatca atcctcttac aaaatataag tggaaacgta aagtaagaat
      781 cacgcttcct gcttttttgt gtgctttagt gcccgatgct gcattggcga gtattaaccg
      841 ttatttagaa gaaggcaaac cagaagattt aaatacgtat aaaatggagg atcctgttcg
      901 tttaaaagtg attgtacatg tggggcctaa aggttttcaa aaggtagggc acatttgttt
      961 ttcatatgac aatattgtat atagttatgg caattatgat agtgactcct ttcgtttgaa
     1021 tcaaacaatt ggagatggta tcttttttac ggtgccttta gagaaatata ttccgaatat
     1081 gatttcggct gaaaataatt cgatttttga gtatggtatt ttgacaacgc ctacacaaaa
     1141 tgcattcata gaaaaggaaa ttgaaaagat tcgtttaaat gggtatcgtt ggtatacaaa
     1201 aatagaaaaa gaagatggct atgatcattt tagtcagtat gaaatggatt atccaagtcg
     1261 acttcattat cgtacgggag ctaaattata taaggtaaag agtggtaaat ttcatattta
     1321 ttgggcttta ggtgataatt gtgcttcttt tacagattta gttttaggaa cacttggtgc
     1381 agatgtacta agtatacgtg ggatcattag tccaggaacg tatttggatt ggcttcaaaa
     1441 agaatattta aagaaaaata gtccaattgt atctaggtgt atatatacaa aagaaacggt
     1501 ggaacaatag atgaacggat atttttatgt attaacgaca attgatattt tcgttctttg
     1561 tttcatgtgc gtgttgacgc gattaagtga atcattaaat aaaaaacaaa agcgaggttt
     1621 tttctttgcg tttatattga ttgcgtttat ttctgtattg gaagttgtta cattaaaagt
     1681 ggatggttta ccagttcgtt atcgttggat caatattata tcgaattatc ttggctttgg
     1741 acttacacca tctgtttgta tctgtttggt gtatgtattt gataaaaaga cgaggcttag
     1801 gtgggaattt aaattggcaa ttttatgcga aattatctat cttttagttc ttgcgatatc
     1861 gattccgtct ggacttgtat tttcagtaag tcaagaaaat atatattctc gtgggcctta
     1921 tttctttatt tacatctttg cgtattttat atctatattc tatttgtctt tatctacgat
     1981 cttagtttcg aagcagtttc aaaatcgcag taaagcattg atttatccat tgattatctt
     2041 cttggctgca gaaaccatca ttcaaatcct acttcctaat cttcatgtga gctggctttg
     2101 tgtcacttta ctttctgtac tttattttat ttattgtagt gaaatgtgga atcaattgga
     2161 tagtttaaca ggattgttga atcagaatag tttcttgaat cgtagtcatg aaatgaatta
     2221 tacaaatgga atgttgatag tgtttgatgt gaatgatttt aaacatgtca atgatgtgta
     2281 tggacatgtc aaaggagatc tttgtctaag tatcattgcc aactgtatta agaaagccta
     2341 tgccaaatat ggatattgtt accgaattgg tggcgatgag ttttgtgtat tattaaaaaa
     2401 ttgtcgtaat gaagaatcgt gttctataca atttgattat ttgatgaaat ccaaacaaag
     2461 aaaatataaa tatttgcctt cggtatctta tggatcggct ttgttgctga cgggagatat
     2521 tgtgagtgtt aaggatttgg cagatcaaaa tatgtatcta aataagaaga ggaataaaga
     2581 acagcagtga tttgataatc gctgctgttt taaggtagaa accaagtaat caaatccacg
     2641 atccatgcga tgaaagtaga tcctttctta atttcgttgt catattgatg ggattgtatt
     2701 atttcttctt ttgaaaaacc cttttgtgtt aaatcttcta tttctttttg agttaggcct
     2761 tgattcagta attcatcctt atcttgtttg ttcattttct ttacctctta tgaatggcta
     2821 ttatctttac gatattgcaa tggagaagta tgagtggcct ttttaaaggc ttgtgcaaaa
     2881 taagattgac ttgtaaaacc aacaatatat gcgatttctg aaatttgtag gttggttgta
     2941 gacaacaaaa gtttagcttc ttccaaacgt ttttgaatca tatagttaat gggagaaatg
     3001 cctttatatt gtttaaaggc atgtacaata tggtatttgt ttaagaatgt gagttcactt
     3061 aatatatcga gtgtgatgtc ttctttgaag tgtgcatcaa tatatttttc aattaagacg
     3121 caatctttgt tggctttatt gattggtgaa gattttaagt tgatgtttgc gcgtcgcatg
     3181 aggttgatca ataaaacttc aagtaggttt tgacaaagag attggtattc tgtatctttt
     3241 gtctttactt caaataggat tgttttcaaa taaaacaaaa cttcatgttt atattcacaa
     3301 taattggcaa tactacaacc ttgttcttca tcttcttctt ttgaaaagta agtgacacct
     3361 tcaattccca tgacgatata ttcgaagttt acattttcaa taccacgttc tgtatgagaa
     3421 actaaagggt tgacaataac tagatcatct tctttgacag gatagttttt attatctaga
     3481 cttagtagtc catgacctct tgtcacatag aatatttctg tatataaatg tgtatgcaga
     3541 gtggaattcc attcatgatc aaatttagat tctgttatat ataaaagctt tgggcgtgct
     3601 ttattaaagt ctacttgatc gatttgaaag cgttgtgtac tcatataaac acctcttttt
     3661 gtataattat ataatgtttt catttattta tcaattttgt tcataaattc atcgaatcgc
     3721 aatatatcta aaatatcgag ctatattttt atgataaagc gcttacaaat aagctatact
     3781 ttgattgtca aaagaggttc atacgaattt tatgaggagg taaagtatga aaaaagttat
     3841 aactgctggg ttatcatttg cgatggctgc ttcaatgtta gcaggatgtt caagcaaacc
     3901 agctgagaat tcggattctg gagataaacg agtattaaaa tttgattcgt tctctggtgg
     3961 aaatggtgaa aaagtattta caaagttagc aaaggctttc gaaaaagcaa atccagatgt
     4021 agatgtacaa ttgcgctttg aaaaagaatt gccagatgta ttaaataaag aaaatgcgaa
     4081 gggtgaatat tcagatgttg tttattataa tttaggacag ccttctggat atacggaaac
     4141 tcaattaaat acacacgaag tattagatat ctctgatgtt gttaaagaag taggtatgga
     4201 tgaaaactat gcgaatagta gtatcgtaca atattatggg gatggaaaat cttatttaat
     4261 gccacttaaa acaacacctg ctggattctt ctataataca gaattagttg gagaaggaaa
     4321 gaaatatgat cttccaacaa cttgggatga attctgggct ttaggtgatc aagctaaaca
     4381 agatggtatt tcattattta catatccaac aaacggttac tttgataata cattaaatgc
     4441 attgttaaaa gaaacaggtg gagaagaatt attatctaac gtattaaatt atgataagga
     4501 tgcttggaat acagatggtg ctaaacaaac tatcgatatt ttaactaaat tagtaagtcc
     4561 tgattattta tattcggata cagtatctaa tgccaatgcg aaagatggat tcacaatgaa
     4621 ccaacaggca gttattgatg gaaaagcatt attcatgcct aatggtgact gggtagtgaa
     4681 tgaaatggca gatacaacgc ctgaagatta tcactgggga ttacttccat tgcctgcatt
     4741 agaaaaagat ggtgaacgtt tcgttggatc tatgactgaa caagtgtgga ttcctgctca
     4801 agctaaaaac gtagatgatg cgaaagcatt cttgaagttt atctattctg aagaaggcgt
     4861 taagatcatg gctgaaggtg gaaacattgt tccaacagat actatgtctg aaacaattgc
     4921 agctatggaa gatggatata caaaagaatt cttctcagta tatgataatg cttctgcagc
     4981 tttaggtgca tttgctcctt ataacacaga taacttacca gatttcgatc gtgctgctac
     5041 agtatttggt tcaatcgact ctttagcaac aggtgaatta agtgcaaaag attatcaaac
     5101 taaattatca gatgcttggg ctaaattagc aaaaaataaa gccgtagctg aataattaaa
     5161 aaagtatatt ttaacataaa gcgatgagca tattattgct cgtcgctttt taaaagatgg
     5221 gaggtaactc atgaataaaa agaaagcaca aaatcgtttc gtatttgcgt gtcttgcacc
     5281 agccgttatt ttagttgtca tttttatggt cattccaacg attgatgtat ttaagacggc
     5341 attctttaaa aagagcaatt tcgtaagtga tgccactttt gcaggtttat tcaactttaa
     5401 ggttcttttt aacgatatgc gttttattga ggctatgcaa aatacaattt tatatgttgt
     5461 attgatcaca atcatcactt tggtgttggc agtcttattt gcgaccttat taactcgtga
     5521 agaatttgta ggaaaaaatt tcttccgcgt gattttctat attccaaata ttttgagtat
     5581 tgtagttatc gcaggtattt tctcagccat ttatcaacca accaatggta ttttgaattc
     5641 attcttaggt atgattggtc tagaatcttt acaaagacaa tggatgggaa atccagctat
     5701 tattaactat tctattattg gtgcattagt ttggcaggcc attggatatt atatggttat
     5761 gtatatgtcg agtatgacaa gtattccaga aaatttatat gaagcagcaa gtttagaagg
     5821 ggctagtaaa gtaaaacaat tttttatgat tactttacca ttgatttggg ataacattcg
     5881 tacaactttn nnnnnnnnnt cgttatttct acaattaact tatccttcat gtttgtgcag
     5941 ttgacaagtg aaggaaactt aggaagtgaa gtggcattga actatatgta taacaatgcg
     6001 tttgcgggta aatatggatt tggtatggca atcggagttt gtatcttcgt attctcattt
     6061 gcgttatccg ctattataaa taaagtcaca gaacgtgatg tattgcagta ttaggaggat
     6121 gaaatatgaa gaagcgtaat tttcctttat ataaaatctt tgtttatgta gcattgatta
     6181 cgttagctat ttcgattatt gtaccgatcg catgggtttt ccttgcatct ttaaaatcgc
     6241 aactcgaatt ggtaagtggc aatccatttg attttccaaa acaaatgtta tggtcaaact
     6301 ttgcggatgc ctttgtgaag gcaagaatgg gtgattattt gttgaatacg atcatcgtaa
     6361 ccgcattatc tttagttatc ttgttggtgg ttgctcttcc tgcatcgtat gtattagctc
     6421 gctttgattt tgttggaaag aaattcttta acggattgtt tatggccgga ctatttgtca
     6481 acgtgaatta catcgtatta cctattttct tgatgttatt taatgccgat atcaagcttg
     6541 gcgtcgactt tttcttgaat agtaaattct ggctatctgt cgtttatgca gcaacagcct
     6601 tgccatttac gatttatttg ttgacaggtt atttcaaaac gttgccaaaa gcttatgaag
     6661 aagcagctcg cattgatggt tgtggctatt tcaagactat gactaaaatt atgattccaa
     6721 tggctagacc tagtattatt actgttattt tatttcagtt tttagcattc tggaacgaat
     6781 atattatcgc atttacattt atggataaag ataatgcgac tttggcagtt ggtgttaaat
     6841 atttgatggc cgatgcaaaa tcacaagcga attttggtgt tatgtatgct ggattggtaa
     6901 ttgttatgat tccaacactg attctttata tttgtgttca aaagaagatt acacaaggta
     6961 tgagcattgg tggaatgaaa gagtaagagg tgtctagtat gagagatgat aataaagtaa
     7021 tgaatatagt tatgattatt ttattctttg tgtttttagg actaatcatt tgggcacaaa
     7081 agacagtgag tgttcgtaat ttaatgattg aaattgttgg attgacgggt ttgcttacat
     7141 tgttgttttt atacaacaag aaacataaat aggaggatga agtatggcag aattatcatt
     7201 gcagcatgtt gataaaatat atgacaacaa tgttcaggct gtatttgact ttaatttaga
     7261 tgtaaaagat aaagaattta ttgtgtttgt aggacctagt ggatgtggta agtctacaac
     7321 acttcgtatg attgctggtt tggaagagat tagtgcaggt gatttctata ttgatggaaa
     7381 aagaatgaat gatgtggaac caaaagatcg tgatattgcg atggtattcc aatcctatgc
     7441 tttatatcca catatgagtg tgtatgagaa tatggctttt ggtttaaaac ttcgtaagtt
     7501 cagcaaggaa gaaatagata aacgtgtaca tgaagcggca cgtattttgg aaatcgaacc
     7561 ttatttggat cgtaaaccaa aggcattatc gggtggacaa aggcaacgtg ttgcattagg
     7621 acgtgcgatt gtacgtaatg cgaaagtatt cttgatggat gaaccacttt caaacctaga
     7681 tgctaaattg cgtgttcaaa tgcgttctga aatcattcag ctacatgaaa gaattggtgc
     7741 tactacaatt tatgttacac atgatcaaac agaagctatg acaatggcgg atcgtattgt
     7801 tgttatgaaa ggtggatata ttcaacagat tggtacgcct aaagaaattt ataataatcc
     7861 cgcaaatcta tttgtggcag gattcttggg ggctccagca acaaacttta ttaagggtgt
     7921 ttacaaaaat ggattctttt atatagatga tatgaagatt gatattcctt tgatgtatcg
     7981 cgaaaaactt tctagctatg aagaaaaaga aatcattatg ggtattcgtc cagaagatct
     8041 acatggggaa ggcattgttg ccgatacgta tccaagtgct aactttgatt ttgaaattga
     8101 agtggctgaa ttattaggac atgaatatat tttacatggt tctttgaatg ggcaaaaaat
     8161 gtgtgcaaaa gtaaattctc gtttagaacc agaagcacat acaacaatta aattgactat
     8221 ggatttatca aaagtgcatt tctttgattt agaaacagaa aatagaattg actagtttag
     8281 gagaaaataa aaatgattga aacaggcaga gtaactgtac caacagatgt tgatgtgatt
     8341 gatgcgacaa ttgaaatcat tcatcgatgg aaagccgatg cgattcgtga ctgtgatgga
     8401 acggatatgc cggaagaatt gcgtaaaatg gacatcaaac aatatgcgac ttattataca
     8461 acaagaaaag ataatgcgtg ggctaaggca aatccagatg aaattcaaca aatgtatttg
     8521 atgagtgatt ttgtgacagc taaggatacg gaattaaaga ttcaaatcat gcagcatttc
     8581 tattcagttc aattaaagcc aaacaccaaa gacaatcata gatggtggga agtgattgat
     8641 cgtactacag atgaagttgt cacaaattgg gattatgatg aaaaaacgat ggaagtcatc
     8701 attcatgata ccattccata tcatgcgtat actgtaagct ttttagcttt tgtgatctgg
     8761 gacccagtac atatgtataa tgctctaaca aacgattgga aagatgaaga gcatcagatg
     8821 acttatgatg ttagacagcc taaaacacaa aagtatgttt tagataaatt cagaaaattc
     8881 tgtgaagaaa gagatgatgt ggatgttgtt cgttttacaa cattcttcca ccagtttact
     8941 ttacaatttg atgagtttgc gcgtgaaaaa tttgtagatt ggtttggata tagtgcatct
     9001 gtttctccat atatcttgga acagtttgaa aaagaagtag gttatccatt tagagcggaa
     9061 tatattatta ataagggatt taacaattcc actttctgtg ttcctacaaa ggaatatcgt
     9121 gactttatgg atttccaaca gcgtgaagtg agtaagctag ctaaaaaatt tgtcgatatt
     9181 tgtcatgaat atggaaaaga agccatgatg tttttagggg atcactggat tggtacagaa
     9241 ccatttggta aatattttga atcgattgga ttagatgccg ttgtaggttc ggttggttcg
     9301 ggcactacac ttcgtttgat ttcagatatc aagggtgtta aatatacaga aggaagattc
     9361 ttaccttatt tcttcccaga tgttttccat gaaaatggag atccattaaa agaagctaaa
     9421 gtgaattggg ttacagcaag aagagccatc ttgcgaagtc cagtggatcg aattgggtat
     9481 ggtggatatt taaaacttgc gttggatttc ccagaattta ttgattatat tgaaaaggtt
     9541 tgtgatgaat tccgtacttt atattcacat gtaaatcaac aagttccaca taacttatta
     9601 aaagtgggtg ttatcaatag ttggggtgaa cttcgtcgtt ggggaacgca tttagttcat
     9661 catgcgattt ggtataaaca aatttattca tacacaggtg ttatggaagc attgagtgga
     9721 tttccgtttg atgttcgctt tattagtttt gatgatattc gcaaagatcc atccatttta
     9781 gaggatatgg atgttgtgat gaatattggt tctgcctata caagttttag tggtggtgca
     9841 gaatttgacg aaacaatcat tacagcactt cgtcgctatg ttgatcaagg tggtggattc
     9901 atcggtgttg gtgagccaac agctataaat cgtaatggta aatattttac attatatgat
     9961 gtattaggtg tcgataaaga attaggtttc actatgcata cagacaagta taattgggaa
    10021 gtgcatccaa atcattttat tacggaacaa ttagagcatg aagactgggg agaatgcatc
    10081 aataatgtat atgcactacc tggtacagaa atcttggctg aaaaagattt gcatgtaaat
    10141 ctagctgtga atgaatatgg aaaagggcgc ggtgtgtata tgaatggtct tccttttagt
    10201 tttgaaaatg tacgactctt atatcgtgcc atcttctttg cggcacataa ggaagatgag
    10261 atgaaacatt ggtattcaga gaactataat gtagatgtca atgtatatcc ttctacaaat
    10321 agtttctgtg ttgtaaataa tacatacgaa cctcaaacta caacaattta taagggtgat
    10381 ggttctagtt ttgaagttga attagcagaa tgcgaaattc aatggtttga aatataataa
    10441 tatcctgtaa aagtaaatag agccaagcta aaagtgcaga tttctctgca cttttagctt
    10501 attgtggatg gtaaaataga aatatagggt ggtgattttt atggatagtc ttatttttga
    10561 cgagaaaaag aaagaattta ctgtattgga tggaagttta ggtaagtatt cgttttatga
    10621 tgttgtttca agtcaaattg tatatgaaga cgcaaaatat aaagacaaat cacctatgtt
    10681 ttctcataag gtgttagtaa gtactttgaa tttgagtatt ttcattgaaa tgaaaaaaat
    10741 atatgtgggt attgaaattc aattcttaaa tggaaataag gtatatgtat atatttcgaa
    10801 atctccagtc attcaacata attttcaatt caaacaagac ttgaagattg ccaaatgttt
    10861 gaatgaaaaa ttaaaagaat tctataaata gaaaaaaaga agagtttatc tcttcttttt
    10921 tatcttatgc ataatttttt taaggatcct aattatctaa aattaaatag cgttttgttt
    10981 cttcaatgtt tttaatgttc tagctgaaac acggatagtt tgaggttttc cgtcaactac
    11041 aacacgaact ttttgaagat tcacgttcca tttacggcga gaagcacgca ttgagtgaga
    11101 acgtttattt ccagataaag gaccttttcc agatactgca catactcttg acatcttaat
    11161 gtcctccttt ttctttcatt tacgcaatcg cttaactata ataccattaa caagcctgta
    11221 tttcaagtcc tgaattaaaa tttgttagga attcaataga atgttataat gatagtgttt
    11281 tgaaaagagg agtagataga atgttgaatt cgaattatat atggattatt ggaatgtgtt
    11341 taatcacact cgttattatg ttagtcatca tgcctggatt gattgattat ttacataaaa
    11401 tcaaatttgg acagacggaa agagaagagg gcttggcaag ccataaaaag aaaaatggta
    11461 cgccgactat gggtggctta gcctttatta ttgtgcctac acttgtgtat gtgattggtt
    11521 ctttctttac accatttaaa ttagatatga acacaatgat tattttattg gcctttgtag
    11581 gatatggtgt tgttggattt attgatgatt atattattgt agttcaaaag aacaatgagg
    11641 gattaaaacc agcgcataag tttttacttc aatctgtttt agccgttgta tttttcttct
    11701 tgtaccgaac aaattcaacg acagatattt ggattcctat ttttgatgtc acaatcaatt
    11761 tatcatggtt ctactttatt ttagttttct taatgtttac agcagaaacc aatgccgtta
    11821 acttaacgga tggtttggat ggattatgtg caggtcaaat ggtgatcgca ttagttcctt
    11881 ttgcgctttt cgcattcatt caaggcttaa ataatgtggc atgtttgatt tgtatggtca
    11941 tctttgcgtt actaggctat ttaaaattca ataagcatcc agctaagatc tttatgggcg
    12001 atactggatc tttggcatta ggtggcttta tagcggccat cgcaatggtt ttaaaacaag
    12061 aaatcctttt ggttgtgatt ggatttgtgt ttgtggcaga ggttttaagt gtaattatac
    12121 aggttacgta ttataaaaaa acacataaga gaatctttaa gatggcgcct ttacatcacc
    12181 attttgaaat gtgtggctgg tctgaagaaa aggttgtaac tcgctttaga cttgtaggtg
    12241 tgatcttagc tattcttggg cttgttttgg gggtaatgta gaatggaaac tgtattggtt
    12301 attggcgcgg ctcgtagtgg tattgcggta agtaaattat tattaaagaa tgggtatcat
    12361 gtgattttaa cagattctaa taaagtgaat gaaaagagtg aattagaatc attgggtatt
    12421 gaagtatatg atgaaggtca tccagattgt ttaaaagaaa aaaaatatgc gtttattgta
    12481 aagaatcctg gaatccctta tcgtgttcct ttcgttcatt attttgtgga acaaaacgca
    12541 aaaatctata cggaaattga agtggcatat cgttatgcga aaaactttga ttatgcggct
    12601 gttacaggta cggatggaaa gacaacggta acaactttat tatttgagat gttgaatcgt
    12661 caaaagaaga gtttagttgc cggaaacatc ggtacgcctt tgtgtgaatt ggctttagaa
    12721 tatacggata caaattataa tgtcgcctta gaattgagta attttcaatt gttaggaatt
    12781 gaaacattta gaccacatgt atcgactgtc acaaatctag ccccagatca cttagattat
    12841 atggatagtc tagaggctta ttatgaaagt aagatgcgta tttatgaaaa cacaaatgcg
    12901 gatgattatt ttattcgtaa tgtggatgat gaaaatgttg tgaagtatgc gcaaaatatt
    12961 ccttgtcgcg tgattgattt ttctttaaaa agaaaagatg tggatttata taaagatgat
    13021 caatatgcct attataaaga tacacaattg tttaaattat ccgatttaaa aattgttgga
    13081 gaatttaatt gtggtaatgc gatgatggca agttgtatgg cttacttaat gggtgtttcc
    13141 ctatttaata ttcaagaagt aattcgtgaa tttagtggtg tagaacatcg tattgaatat
    13201 gtggatacaa ttgataatgt gcgtttctat aatgattcaa aggctacaaa tacgcatgcg
    13261 gcttgtgctg gattatccgc ttttgaaaag aatgtgattt tactttgtgg tggtaaagat
    13321 aaacatattt cttttgatga tttgaaacaa tatgatgcgg ttgtgaagaa atgtatctct
    13381 tttggtcaga caaaagataa atttaaagat attttttcga atcaagtgag tgttgaaact
    13441 atgaaggatg cgtttgaaaa ggctgtcttg tttgcgaaac ctggtgatat tgtcttgttg
    13501 tgtcctgcat gttctagttt tgatcaattt aaaaattatg agattcgtgg agaaatcttt
    13561 aaagagatgg ttcatgatta tgcatcaaaa aggaatgaag aatgatagat attattaaaa
    13621 caatcttttt aggtatcctt caaggaatta ccgaatggtt acctatttct tcaacaggac
    13681 atttgatttt attttctagc atttggcctt tagagccggc taaatttttt gaagtgttca
    13741 aagttgtcat tcaatttggt agtattttgg ctgttttagt tttgtatgag cagaaattga
    13801 atccatttga ttcaagaaaa tcagctaaga agaagaaaca gacattacaa ctgtggtcaa
    13861 aggtgattgt aggagtgatt cctgcttcca tcattggttt attattggat aatatcgttg
    13921 aggattattt atcaaagaat attgtgattg cgatcacttt gattctatat ggaatgatct
    13981 ttattttcgt agaaaaacat ccaaagaaag aaaaggttca aaccattgag cgtttggatt
    14041 acaaatcggc tttaaagatt ggttgttttc aatgtttggc tttaattcca ggtacatcaa
    14101 gatctggagc cacgattttg ggcggtttat attgtggttg ttcaagaaca gtcgcaagtg
    14161 aattttcctt ctttttggca attcctgtta tgtttggtgc gagtctatta aaagtcatca
    14221 aatatttgat gaaggttgga ttgtttagtt ttggacaatt attcttattg ttgttaggta
    14281 cttttgtagc ttttgtggtc agcttatttg cgataaaagc tttattaaat tatgtaaaga
    14341 atcatgattt tactgttttt ggatggtatc gcattattct tggtattttt gtgattttat
    14401 ttttctatat attataagag gtaagtatga atagatttga tttgcatatg cattcttgtt
    14461 attcgagcga tggtcaattc acgccaaaag aattgattga aatcgctaaa gataaagggt
    14521 taaagaccat tgcgcttagt gatcatgatt gtattagtgg tgtaaaagaa atgattcgct
    14581 taggaaatga agttggaatt gaagtgattc cagctattga gtgttcaaca agcattggat
    14641 atacagatgt gcatttactt gggtatggta ttgatttaga taatgattat tttcaaaatt
    14701 tattagaaaa aacgcaagta aagtctagaa atgcattttc aactcgttgt gatcgattgc
    14761 gtgcaaaata tggggttgaa attgatgaag aggcgatatt aaaagaggcg aatggtaaaa
    14821 atccatggtt tgtgatgtgt acacatatgt ttaatgatcc tcgttatcaa gatattccag
    14881 attttaagga ttatattcct ggcggtaaaa gaagtgatcc ggctcctgtg aatttctttt
    14941 gggataaatg tcaatatgga tcggacttgt ttgtatatgt acctatgcct gattttgtcg
    15001 aatcggttca aaagattcat gaggctggtg gactcgctat tgttgcacat ccttttacaa
    15061 cgttttataa aaacgatgag atgttgaaaa ttttattgga ttcgggagtg gatggtattg
    15121 aagcttatag taattatcat acgccagaac agaatgtata ttatgaaaat cttgccaaag
    15181 aacatcattt attgattact tgtggaagtg actttcatgg ggagaaaaag cctagtattg
    15241 aaatgggtga gtatggttta gaaaaagatg gttcggtttg gctagaacga attaaggaaa
    15301 aaataaaggc agctagataa tctagctgcc tacattgtta ataagaatgt aaatagaatg
    15361 caataactta atagattgtt tgaacgactt gctctaaata gggattggtg tcccatttct
    15421 gtaatggcac aaaatttatc tacagcttga ataatccatc caacacttgt gtttggatga
    15481 cttgtcttta gtggccacat gtgatgtaaa atggcatcat caatggttgg attggttact
    15541 gtaatatttt ttgcgttttc tagagcgatg attggatgaa tcttggcgtg atttccttct
    15601 aaaggacgtt tgtcattggt tttccaatca tagtaatata ggtcatgtaa aagaccagca
    15661 cgggctgcac tataggcgtc gagtccgaat tttctacaaa tcaaaaatga ataataggaa
    15721 acattcaatg aatgttgaaa gcgggatgta gctaagtgtt ggctgcattt ttttaaatct
    15781 tgaacttctt ttgtatcaat taaatctgaa attgtttcaa aataatcata tgcgatattt
    15841 gtatctaatg
//
LOCUS       DBMDWL010000029        15834 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1252_length_15834_cov_2.639738, whole genome shotgun sequence.
ACCESSION   DBMDWL010000029 DBMDWL010000000
VERSION     DBMDWL010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 15834)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15834)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15834
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_15834_cov_2.639738"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="ACZPDN_03370"
     CDS             <1..1621
                     /locus_tag="ACZPDN_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIR1297694.1"
                     /translation="LFFEDCSIYDLSELLGLSDSVIRKEINKVIQFLLKNYNVELFKL
                     KKDECVILLEESEIRELLFKIVKDTALENPDALMDILYMLLSDSWVMKYYKSTKEDVI
                     KIMNGEQVYLSETDLSIFAACILICRVRNLNGYNLEDNQISSPDDLYTRVITKLCMQS
                     SYLEQADIPILYNLLHTFKMESTDEVPKITDFTVLVFDEFCNEVFDKYSINLKESEAL
                     SSKMLLHIEFMNRRVIGGYELKNPIVDDVKTKFPFAFEISMMIVPILFKYKRVYVTED
                     EISYLTVYVAQFLENENVKLKTIVVTSQRHSVKQLLTQWLEMYFKNQIAIVDIINKEA
                     LKKMDLTSIDLVITLDSFLILKDVEVFSMDKLPEIKDIERLNSMIHMIRMNKRVSKIL
                     DCYIQKEHVKVYPDTKELPELLQEMSQKLHESGFISDTKGFYEDVLLREKNYPTNLGS
                     QMMVPHALFTFADKTGIEVALLKKPFEHHGNQVQLVFLLALEKKRNDEMNLLFQFFNQ
                     IVSHKKYMHELLQSEDSDAFIKNLYSFKLLE"
     gene            1693..3021
                     /locus_tag="ACZPDN_03375"
     CDS             1693..3021
                     /locus_tag="ACZPDN_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HIR1297695.1"
                     /translation="MKKFSIVIAGGGSTYTPEIILMLLDNLDRLPLRSIKLYDNDEER
                     QNHVAKACEILIKEKDPTIEYVATTDPEVAYTDVDFVLAHIRVGKLGMRSLDEKIPLK
                     YGVVGQETCGPGGMAYGLRSIPGVLENIEYMEKYSPNAWMLNYSNPASIVAEACRRFK
                     PNSRVINICDMPIGMEHTIARILGFKSRKEMCIRYYGLNHFGWWTSIKDKDGKEYIQD
                     LIKHQLKYGNCLADDDASNYTDSSWFDTAKKVKDIIAIDPTMCPNSYFQYYLFGDDMV
                     AHTDPNYTRADQVVDTREKRVFGECARIEKVGTAKDTTLQIGIHAEFIVDLATALAFN
                     THERMLLIVPNNGAISNFENDAMVEIPCIVGKDGYEPLTVGEIPHFQKGLMEQQVNCE
                     KLVVDAYEQHSYLKMLQALTLNKCVPNANVARKILDDFIEANKAYWPELK"
     gene            3037..3330
                     /locus_tag="ACZPDN_03380"
     CDS             3037..3330
                     /locus_tag="ACZPDN_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1297696.1"
                     /translation="MKILLCCNAGMSSSILVKKIRDVAEKTGEDIEVSAVANASIKNE
                     VGKWDVCLVGPQLVFAVDSIHQQLNIPVASIEPRVYAMADGAAAIEMAKKLLG"
     gene            3332..3652
                     /locus_tag="ACZPDN_03385"
     CDS             3332..3652
                     /locus_tag="ACZPDN_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIR1297697.1"
                     /translation="MANVEQISQVAMQVITYAGMAKSSYLEALKYYRENDQTAYEQSL
                     SNGDESFTQAHEAHLQLLQQEMNTQEPQITMLMAHAEDQLMSAETIKTLIQEIILLIE
                     SRGK"
     gene            3654..4250
                     /locus_tag="ACZPDN_03390"
     CDS             3654..4250
                     /locus_tag="ACZPDN_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297698.1"
                     /translation="MTIWADIQTKNGFASDEVQSSLQKYIRRAKVEEACQAAYELYTS
                     GPVFLDKMWSRLETIAVEDVGFGDLNVPVLIHALDQMRKNFPYNDGDQPMYFIHAIRV
                     LCACTKDRSSDYLKNIIIKESAMGKIMEVPDIALDKHTKRGQEMGRGSKHFFEEATKV
                     IPQLEIDNDYRERYGKILETYDPDHVVENAFKYSSEQY"
     gene            4273..4875
                     /locus_tag="ACZPDN_03395"
     CDS             4273..4875
                     /locus_tag="ACZPDN_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1297699.1"
                     /translation="MSYNIWAAAKTRNGLAADEVISTLQKSIRRNKVEEACQAAYELY
                     ITGPLFLDKLWRRLLTISVEDIGFGNLQAAVQVNTLNEVRKSYAYDDGDQPMYFIHAI
                     RLLCASTKDRSSDYLKNIIIKEAAMGKIMEVPDIALDKHTKRGQEMGRGSKHFFEEGT
                     KVIPQLKIDNDYRERYGKILEKYDPNHVVPNAFKYNPGQY"
     gene            4922..5638
                     /gene="deoD"
                     /locus_tag="ACZPDN_03400"
     CDS             4922..5638
                     /gene="deoD"
                     /locus_tag="ACZPDN_03400"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1297700.1"
                     /translation="MSTAHNKASLEDVAKVVLMPGDPLRAKLIAENFLENPVLFNDVR
                     GMLGYTGTYKGKRVSVMGSGMGMASMGIYSYELFKFYDVDAIIRVGSTGSYSERLPMK
                     SVALVKGSYSESTYALAQSGDTDTMQYPDEDLNKLIESVSQEVSIPVVKTNTHSSDVF
                     YYEGDLEQPEKMVEEQDALCVEMESFALFHNAKVLNKKAACLLTVSDSLCTNEHLSAD
                     ERQTSFNEMITLALESAIRL"
     gene            5635..6819
                     /locus_tag="ACZPDN_03405"
     CDS             5635..6819
                     /locus_tag="ACZPDN_03405"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1297701.1"
                     /translation="MKYKKIVCVVIDSFGIGQATDAKEFDDVGADTFGHILEYRPDLK
                     IDNLYKLGLGNLHPNGKNLQSKGYACKMHEASCSKDTMTGHWEMMGIHTTKPFKTFTE
                     NGFPDELVQELERLTGHVFIGNKSASGTEILDELAMEEIQSDGKKLILYTSADSVLQI
                     CGHEEVTGLDELYRVCQIARELTLKPEYKVGRVIARPYVGDSIGHFTRTSNRHDYALS
                     PSSETVLDVLKSNALDVIGIGKIRDIFNGQGITKSIHSTSSHEGMLQTIEEMKKDFTG
                     LLFTNLVDFDAKWGHRRNVSGYADELEDFDVLLGQLLSVLDSDTLLIITADHGNDPTY
                     KGTDHTRECVPCLMYSPSMKEHGILDDSSSFGVLGASVLDNFGLSKPTDLIGESILDL
                     TK"
     gene            6906..8225
                     /locus_tag="ACZPDN_03410"
     CDS             6906..8225
                     /locus_tag="ACZPDN_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297702.1"
                     /translation="MNKMEHMEIKQLVVSMSLPIIISMVIQALYNIVDSIFVAKVSSD
                     ALTAVSLCYPVQTIMVAIACGTAVGFNTLLARYLGEKKFDYANNTMMHGILLGFVNGL
                     VFLILGLLFSNVFLSWFTSDSNVLSLANTYIHICTMFSFSVFVQIIFERIMQATGNAI
                     YNMVMQGAGAIINIILDPILIFGWFGLPEMGVAGAAIATVIGQFCAMLIGYVITKVKI
                     KELDIHIHNFSFSIPILCKIYKVGVPAILMQSVLSFMTVFMNMILAPISAMAITVFSV
                     YYKLQNFLNMAVLGITNALIPIVAYNNGANRKDRAIEAIQFSMILSIFIMAVGTIVFQ
                     LFPKQLLAMFSANEEMYGLGVPALKIISLSFVFAGISMVLCAAFQALNKANTSLVITL
                     ARQLVILIPLTYGLMKCFGIHVGWYAFVVTEFICTAYSLFEWRKMKR"
     gene            complement(8232..8966)
                     /locus_tag="ACZPDN_03415"
     CDS             complement(8232..8966)
                     /locus_tag="ACZPDN_03415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1297703.1"
                     /translation="MIQSINNKKIKEYAKLIQKKERDKTNLFLVEGEHMVKEAFHANA
                     LQELFILEEIECPIQFNYETVTQQVLNKLSNQNSNSKMIGVCNKLNLKPEKEENILLL
                     DHVQDPGNVGTILRTAHSFGIDCIYMSKGCADIYNPKTIQSSQGALFYIPVIQTNLVE
                     EIKKLQERNIEVYATALHDKHKDLQDIKPSEKYAILVGNEGQGVRKELIDISDHIVKI
                     EMETFESLNVAIATSICMYTFKYAKR"
     gene            9269..10303
                     /gene="pheS"
                     /locus_tag="ACZPDN_03420"
     CDS             9269..10303
                     /gene="pheS"
                     /locus_tag="ACZPDN_03420"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1297704.1"
                     /translation="MKTIEELLQEATQKIQDVKDSSSLNDLRVLYLGKKGPVQELMKG
                     MKDLSKEEKPIFGQKVNQLKQDLSKMIEEKKEQLAAFEMQKKIESEKIDITLPGRKPE
                     LGNAHPLNLVRQELEDLFIGLGYQVIEGDDIVDDEYCFERANIPKDHPARDMQDSLYI
                     DPNRLLRTHTTAIQMVVLEESAPNLPIKVVCPGKVYRRDDDDATHSHQFMQMEGLVIG
                     EKVTLADLKGTLEFMARKLFGQDRQIRFRPSYFPFTEPSVEVDVSCHICNGKGCPTCK
                     GTGWIEILGAGEVHPNVLKMAGYDPEKCSGFAFGVGIERVAMLKYGIDDIRHFYTNDK
                     RFNSTFKRYE"
     gene            10316..12703
                     /gene="pheT"
                     /locus_tag="ACZPDN_03425"
     CDS             10316..12703
                     /gene="pheT"
                     /locus_tag="ACZPDN_03425"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1297705.1"
                     /translation="MRISRKWFNQFMDVQDLSIEEMAKRITDAGFEVEGIEHLSQGTN
                     LVIGHVETCTEHPDSEHLHCTTVNVGNEVVNIVCGAPNVAAGQNVIVALPGAVLPGGE
                     IKNGVIRGVESNGMICSLLELGVDPSSLNDDQKNGIEVLPADAPVGNTDPLGYLGLDD
                     EVLDIGLTPNRNDCLAAFNMAKEAGAILNREVKLPEYNGASDVGSKTHLHVESTTSKC
                     PLFYGKVINHLTIKESPKWMKELLAASGVKSINNVVDISNIVMLETGQPMHFYDAKSL
                     PSQSIVVADGFDEEYTALDGVTYKLQPEDIVITNQGKPIGIAGVMGGDDSKILDTTDS
                     IIIECAIFDHVSIRNTARRLNLSTDASVRYQKGIEPLASKKALDRAVQLLIEYADATG
                     IEETVQYGQVPYEPKSISCTLKAINNRLGTDFKLDEVIDVFTRLDFEPEVADDLITCH
                     IPSSRTDMEGMADLSEEVIRMLGYDRLPSTLPVMPMTEGKLTYKQQLTRQARQFLCAQ
                     GLQDCLTYTLISTEKKENAIFNTDEAIELASPMSEERRYIRTSILPSLLDVVSYNRAR
                     NIKDVNVFELSDVAGVSGAKMHLAIAMSGNLQQTRWTNDVTVSDFYTLKGLVEAFLEQ
                     IGIAAQRISFVENDLDTTHFHPYRSAKIMLGKDCVGLLGDIHPQYGKNIVMAELDFDA
                     LIDVKKSKIKFSPVSKYPSVSRDLAFVLDRAMPVQKVVDTINKQGRLNKEMIIRDVEV
                     FDVYEGEHVSKDEKSVALSITFQSDSHTLKDEEINQVFEAILEHIQKDCNATLRS"
     gene            12797..14278
                     /gene="glpK"
                     /locus_tag="ACZPDN_03430"
     CDS             12797..14278
                     /gene="glpK"
                     /locus_tag="ACZPDN_03430"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR1297706.1"
                     /translation="MQKYILAIDHGTTSTRAVLFDKVANVVEIAQKETTISYPHPGWV
                     EQDANEIWLACLAVMSEVILKSKISPEQVAAIGISNQRETSVLWDASTGLPVSPAIVW
                     QSKQTKSICDGWKKKGYEDIVKAKTGLRIDPYFSASKIRFIFDQNPDVYEKAKKGEVL
                     FGTMDSWIVWKLSGSLTHITDVSNASRTLLFNIHTLSWDDELLEMFDIPKCILPQVKP
                     TSYAYCKTAAYHFFGQEVPICCVVGDQQAALFGQGCFGAGGIKNTYGTGGFMLMNTGK
                     RIVESKNGLLSTVAWQIGDQVNYALEGSIFVSGSLMKWLRDQMQLFENTKDTQAMAES
                     VENTNGVYIVPAFVGLGAPYWDDACQASILGLTFGANKNHIVRAALESMAYQSKDVLE
                     AMRQDSQIDITSMKVDGGACANDFLLQFQSDLLQMPVQRFKTNELTALGAAYLAGLSC
                     GYWTQDELGVELSKEFVPEKSKEEMDSLYSNWKKAVKTCQSYK"
     gene            14295..15008
                     /locus_tag="ACZPDN_03435"
     CDS             14295..15008
                     /locus_tag="ACZPDN_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014571.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HIR1297707.1"
                     /translation="MEIYTKEDFKQIFIDLGIQKGSKVLLEADLNRYSGLVGNVQMLI
                     ETLQELIGDTGLLCMPCFSYSCLDPACLENYYPYEKWKEVRENLPGFHVIFTPSEIYK
                     DCTNLFLRYKDVYRSNHPVYSFAYWGNFDEDVLKVDVNDALSFHGSLSFLNEKNACNL
                     LVGVLPEKSLMVQALSNEYQLGKTIVQRAFLRSKRQLTKSFIVQVVDEEISRDLLDGL
                     TVLESESSLEDVYVLRKGE"
     gene            complement(14992..15438)
                     /locus_tag="ACZPDN_03440"
     CDS             complement(14992..15438)
                     /locus_tag="ACZPDN_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR1297708.1"
                     /translation="MNEILIEQCKYLVDETLPNIANISNLIALLFHEMENINWLGFYI
                     CDEKNQECILGPFQGKVACTRIPYGKGVVGTCAKTQETQRIEDVHKFAGHIACDCASN
                     SEICIPIKKDDKLVAILDIDSFEFNNFSIEDQAILEKICEFFIPLF"
BASE COUNT         5306 a         2237 c         3231 g         5060 t
ORIGIN      
        1 actattcttt gaggattgca gtatatatga tttatctgaa ttattaggtt tatccgattc
       61 tgtaatccgc aaagaaatta ataaagtgat tcagtttttg ttgaaaaact acaatgtgga
      121 attgttcaaa ctaaaaaaag atgagtgtgt cattttatta gaagaaagtg aaattcgtga
      181 acttcttttt aaaatcgtaa aagatacggc actcgaaaat ccagatgcat taatggacat
      241 tctttatatg cttctaagtg attcgtgggt tatgaaatat tataaatcca caaaggaaga
      301 tgtaatcaaa atcatgaatg gagaacaagt ctatttatca gaaacggact tatccatttt
      361 tgcagcatgc atcttgatat gtcgagtacg aaatctaaat ggatacaatc tcgaagataa
      421 tcaaataagt tctccggacg atctttatac tcgtgtcatt actaaattat gtatgcagtc
      481 gtcttattta gagcaggcgg atattccaat tctatataat cttttacata catttaaaat
      541 ggaatcaacg gatgaagtac ccaaaatcac ggattttaca gtgcttgtat ttgatgaatt
      601 ctgtaatgaa gtatttgata aatattcaat caacttaaag gaatcggaag ctttatctag
      661 taaaatgctt ttgcacattg aatttatgaa tcgacgcgtt attggtggat atgaattaaa
      721 aaatcccatt gtggatgatg ttaagacaaa gtttccattt gcgtttgaaa tcagcatgat
      781 gattgttccc attcttttca aatacaaacg tgtttatgtt acagaagatg aaatttcata
      841 tttaactgta tatgtagcac aatttttaga aaatgaaaat gtaaagctaa aaacaatcgt
      901 tgtcacaagt cagcgtcaca gcgttaaaca attgttgaca caatggcttg aaatgtattt
      961 taaaaaccag attgcgattg tagatatcat aaataaggaa gccttgaaaa agatggatct
     1021 tacatccatt gatttagtca ttacgttgga ttcatttctg atcttaaaag atgttgaagt
     1081 attcagtatg gataagttgc cagaaatcaa agatattgaa cgattgaatt caatgatcca
     1141 catgattcgt atgaataaac gtgtttctaa gatattagat tgttatattc aaaaggaaca
     1201 tgtaaaggta tatccagaca caaaagagct tccagaactt ttacaagaga tgtctcaaaa
     1261 gttacatgaa tcaggattca tatccgatac aaaaggattc tatgaagatg ttttgttgag
     1321 agaaaagaat tatccaacta atttaggatc acaaatgatg gttccacatg ccttgtttac
     1381 atttgccgat aaaacaggta tagaagtggc cttgttgaaa aaaccatttg aacatcatgg
     1441 aaatcaagtg cagctcgtat tcttattagc actcgaaaag aaacgaaacg atgaaatgaa
     1501 cttgttgttt caattcttta accaaattgt ttcacacaaa aaatatatgc atgaacttct
     1561 tcaaagtgaa gatagtgatg ctttcattaa aaacttatat agttttaaat tgttagagta
     1621 aaaaaaacaa taaaatcaaa cataatatat gtatcaattt attgtcttat gaaaagaaag
     1681 tgagatagta aaatgaagaa attttcaatt gttattgcag gtggaggaag tacatatact
     1741 ccagaaatta ttttaatgtt gttagacaac ttagatcgtt taccattaag aagtattaaa
     1801 ttatatgata acgatgaaga acgtcaaaat catgtcgcaa aggcatgcga aatcttgatc
     1861 aaagaaaaag atccaacgat tgaatatgtc gcaacaacag atcctgaagt agcttataca
     1921 gatgttgatt ttgtattagc acacatccgt gtaggtaaac ttggaatgcg atctttagat
     1981 gaaaagattc cattgaaata tggtgttgtt ggacaagaaa catgtggtcc tggtggaatg
     2041 gcttatggat tacgaagcat tccgggagta ttggaaaaca ttgaatatat ggaaaaatat
     2101 tcaccaaatg cgtggatgtt aaattattcc aatccggcta gtattgtagc agaggcatgt
     2161 cgtcgtttta aaccaaactc aagagttatc aatatttgcg atatgccaat tggtatggag
     2221 catacaattg cacgtattct aggtttcaaa agccgtaaag aaatgtgcat ccgttattat
     2281 ggattgaatc atttcggatg gtggacatcc atcaaggata aagatggaaa agaatatatt
     2341 caagacttga ttaaacacca gttaaaatat ggaaactgtt tagcagatga tgatgcatca
     2401 aattatacag atagttcatg gtttgataca gctaaaaagg taaaagatat tattgcgatt
     2461 gatccaacga tgtgtccaaa ttcatatttc caatactatt tatttggaga tgatatggtg
     2521 gcacatacag atccaaatta tactcgtgct gatcaagttg tggatacaag agaaaaacgt
     2581 gtatttggtg aatgtgctcg tattgaaaaa gtaggtactg caaaggatac aacactacaa
     2641 atcggtatcc atgctgaatt tatcgtagat ctagcaacag ccttagcatt taatacacat
     2701 gaaagaatgt tgttgattgt tccaaataac ggagcgattt caaactttga aaacgatgct
     2761 atggttgaaa ttccttgtat tgttggaaaa gatggatatg aaccattgac tgtaggtgaa
     2821 attccacatt tccaaaaggg attgatggaa caacaagtga attgtgaaaa gcttgttgtc
     2881 gatgcgtatg agcagcacag ttatttaaaa atgttacagg cattaacttt aaataaatgt
     2941 gttccaaatg ccaatgtggc aagaaaaatt ttagatgatt ttattgaggc aaataaagca
     3001 tattggccag aattaaaata gaaagaggaa atcaaaatga aaatattgtt atgctgtaat
     3061 gcagggatgt caagttcaat tcttgtgaaa aaaattagag atgttgccga aaaaacaggt
     3121 gaagatattg aagtaagtgc agtcgcaaat gcttctatta aaaatgaagt gggtaaatgg
     3181 gatgtatgct tagtaggtcc tcaattggtt tttgcggtgg attctattca tcaacaatta
     3241 aatattccag ttgcaagtat tgaacctaga gtatacgcaa tggcagatgg agctgctgct
     3301 attgaaatgg caaagaaatt attagggtaa tatggctaac gttgaacaaa tatcacaagt
     3361 tgctatgcag gtcatcacat atgctggtat ggcaaaatca agttatttag aagctcttaa
     3421 atattatcgt gaaaacgatc aaacagctta tgaacaatca ttgtcaaatg gagatgaaag
     3481 ttttacccaa gcccacgaag cccatcttca attgttacag caggaaatga atacgcaaga
     3541 accacaaatc acaatgttga tggcgcatgc ggaagatcag cttatgtcag cagaaacaat
     3601 aaagactttg attcaagaaa taattttatt aatagaaagt agaggcaaat aaaatgacaa
     3661 tttgggcaga cattcagaca aagaatggct ttgcatctga cgaagtacaa tcttcattac
     3721 agaagtatat tcgtcgtgca aaagttgaag aagcttgtca agcagcctat gaattatata
     3781 cttcagggcc agtcttctta gacaaaatgt ggtcgcgtct agaaacgatt gcagttgaag
     3841 atgttggatt tggagattta aatgttccag ttttaattca tgcattagat caaatgcgta
     3901 aaaacttccc atacaatgat ggggatcaac caatgtattt tatccatgcg attcgcgtac
     3961 tttgtgcatg tacaaaggat cgttcaagtg attatttgaa aaatatcatt attaaggaat
     4021 ctgctatggg aaaaatcatg gaagtaccag atatcgcctt ggataaacac acgaaacgtg
     4081 gacaagaaat gggacgtgga tcgaaacact tctttgaaga agccacaaag gtgattccac
     4141 aattagaaat tgataatgat tatcgtgaac gctatggaaa gattcttgaa acatatgatc
     4201 cagatcatgt tgttgagaat gcattcaaat atagttcaga acaatattaa tggaaagtga
     4261 gtgtaagaga atatgtcata taatatttgg gctgccgcaa aaacaagaaa tggcttagcc
     4321 gcagatgaag tgatttctac acttcaaaaa agcattcgac gcaataaagt agaagaagca
     4381 tgtcaagctg cttatgaatt atatatcaca ggacctttgt ttttagataa gctatggcgt
     4441 cgattactaa caatttcagt tgaagatatt ggttttggaa atttacaagc ggctgtgcaa
     4501 gtcaacacat taaatgaagt acgaaaatcg tatgcgtatg atgatggaga tcagccaatg
     4561 tattttatcc atgcgattcg tttactttgt gcaagtacaa aggatcgttc aagtgattat
     4621 ttaaagaata ttattattaa agaagctgcc atgggaaaaa tcatggaagt tccagatatt
     4681 gctttggata aacacactaa acgtggacaa gaaatgggac gaggatcaaa acacttcttt
     4741 gaagagggaa caaaggtcat tcctcaatta aaaatcgata atgattatcg tgaaagatat
     4801 ggtaaaattt tagagaagta tgatccaaat catgttgtac ctaacgcatt taagtataac
     4861 ccaggacaat attaagattt aaaagaccag ctttgactgg tctttatttt aggaggaaat
     4921 tatgtcaaca gcacacaata aagcaagttt agaagatgtc gcaaaagtcg ttttaatgcc
     4981 aggtgatcca ttaagagcta aattgattgc cgagaatttt ttagaaaatc ctgttttgtt
     5041 taatgacgta cgaggcatgc taggatatac agggacttat aaaggaaaaa gagtttctgt
     5101 catgggaagc ggaatgggaa tggcaagtat gggaatttat tcgtatgaac tttttaaatt
     5161 ctatgatgtg gatgccatta tacgagttgg tagtacagga agttattctg aaagattacc
     5221 gatgaagtct gtagctttag tcaaaggaag ttattcagaa tcaacctatg ccttagcaca
     5281 aagtggcgat acagatacaa tgcaatatcc agatgaagat ttaaataagt tgattgaaag
     5341 tgtaagtcaa gaagtttcaa ttcctgttgt aaagacaaat acacattcta gtgatgtgtt
     5401 ctactatgaa ggcgatttag aacaaccaga aaaaatggtc gaagaacagg atgcgctttg
     5461 tgtggaaatg gaaagttttg ccctattcca caatgccaag gtattgaata aaaaggcggc
     5521 ttgtttatta actgtatcgg attctttatg tacaaatgaa catttaagtg cggatgaaag
     5581 acaaacatcc tttaatgaaa tgattaccct tgccttagaa agtgcaattc gcttatgaaa
     5641 tacaaaaaaa tagtttgtgt cgttattgat tcttttggga ttggacaagc tacagatgcc
     5701 aaagaatttg atgatgtagg tgccgatact tttggacata tcttagaata tcgcccggat
     5761 ttaaagatag acaatttata taaattaggt ttaggaaatc ttcatcctaa tggtaaaaat
     5821 ttacaatcaa aaggctatgc ttgtaagatg catgaggcaa gttgttccaa agatacaatg
     5881 acaggtcatt gggaaatgat gggaattcat acgacaaaac catttaaaac atttactgaa
     5941 aatggttttc ctgatgaatt ggttcaggaa ttagaaagat taacaggcca tgtctttatt
     6001 ggaaataaaa gtgcaagtgg aacggaaatt ttagatgagt tggcgatgga agagattcaa
     6061 tcggatggaa agaaattgat actttataca tcggccgata gtgttctaca aatttgtgga
     6121 catgaagaag tgacaggact tgatgaattg tatcgtgttt gtcaaattgc tcgagaactc
     6181 actttaaaac cagaatataa agtaggaaga gttattgcac gtccttatgt tggagattca
     6241 attgggcact ttacacgcac tagcaatcgt catgattatg cattgtctcc atcgagtgaa
     6301 accgttttag atgttttaaa atcaaatgca ttggatgtga ttggtattgg taagattcgt
     6361 gatatcttta atgggcaggg aatcacaaaa agtattcatt cgacttcaag tcatgagggt
     6421 atgcttcaaa cgattgaaga aatgaaaaaa gattttacag gtttattatt tacgaacctt
     6481 gtggattttg atgccaaatg gggccatcga agaaatgttt ctggatatgc cgatgagcta
     6541 gaagattttg atgtcttgct agggcaatta ctttcggttc ttgattcaga tacactattg
     6601 attataacgg ccgatcatgg aaatgatcca acctataaag gaacggatca tactcgagag
     6661 tgtgtaccat gtttaatgta ttcaccaagt atgaaagaac atggaatatt agacgattca
     6721 agttcgtttg gtgttcttgg tgcaagtgtt ttagataact ttggattgtc aaaaccaacg
     6781 gatttgattg gtgagtctat tcttgatttg acaaaataga aaaaatatat taatttattt
     6841 tagcgtttta ttttcgtttt ggtatcccct atgaaactgc caaaacatgg gggaggtata
     6901 atttaatgaa taaaatggaa catatggaaa tcaaacaatt ggtggtttct atgagtttgc
     6961 ccatcatcat ctcgatggtg attcaagctt tatataatat tgtcgatagt atctttgtcg
     7021 caaaggtatc gagtgatgca ctaaccgctg tctctttatg ttatcctgtg caaaccatta
     7081 tggttgcgat tgcatgtggt acagctgttg gctttaatac actactcgct cgttatttag
     7141 gtgaaaagaa atttgattat gcgaataata ctatgatgca tggaatcttg ttaggatttg
     7201 tgaatggact tgtctttttg attttaggtt tactattttc taatgtcttt ttatcttggt
     7261 ttacatccga ttcaaatgtg ctttcactag ccaatacata tattcatata tgtacgatgt
     7321 tttcatttag tgtctttgta caaattattt ttgaaagaat catgcaggca acaggaaatg
     7381 ccatttacaa catggttatg caaggagcag gagccattat taacattatt ctagatccaa
     7441 tcttgatctt tggatggttt ggtctacctg aaatgggggt tgccggagct gccattgcga
     7501 ccgttattgg acagttttgt gccatgctta ttggatatgt tattacgaag gtaaaaataa
     7561 aagaattgga tattcatata cataattttt ctttttcgat acctattcta tgtaaaatct
     7621 ataaagtagg tgtacctgcc atattaatgc agtccgtatt aagctttatg actgttttta
     7681 tgaatatgat tctagcacca atctcagcta tggcgattac tgtctttagt gtttactata
     7741 aattacagaa tttcttgaat atggccgtgc ttggaatcac caacgcctta attccaattg
     7801 ttgcctataa taatggggcc aacagaaaag atcgagcaat tgaagctata caattttcta
     7861 tgattttatc gattttcatt atggctgtag gaacaattgt atttcaatta ttccctaaac
     7921 aattattagc catgtttagt gcgaatgaag aaatgtatgg attaggtgtt ccagcattaa
     7981 aaatcattag cttaagcttt gtgtttgccg gtatcagtat ggtactatgt gctgcctttc
     8041 aggctttaaa taaggcaaat acttcgttag tgatcacttt ggctagacaa ctagttatct
     8101 taattccatt gacatatgga ttgatgaaat gtttcggcat tcatgtaggt tggtatgctt
     8161 ttgtggttac ggaatttata tgtaccgcgt attcattatt tgaatggaga aaaatgaaaa
     8221 ggtagcattc gctacctttt tgcgtattta aatgtataca tacaaatgga tgtggcaatg
     8281 gccacgttta aactttcaaa agtttccatt tcaattttga caatgtgatc gcttatatca
     8341 attaattctt tacgaacacc ttgaccttcg ttgccgacta gaattgcgta tttttcactt
     8401 ggtttaatat cttgaagatc tttatgtttg tcatgtaaag ctgtggcata cacttcaata
     8461 tttctttctt gaagcttttt aatttcttct actaaattcg tttgaatcac aggaatatag
     8521 aaaagggctc cttgactgga ctgaatcgtt tttggattat agatatccgc gcatcctttt
     8581 gacatataaa tgcagtcgat accaaaagag tgggcggttc tcaaaatcgt tcctacgttg
     8641 cctggatctt gtacatgatc tagtaataga atattttctt ctttctctgg ctttaagttt
     8701 agtttgttac atacaccaat catctttgaa tttgaattct gatttgacaa tttatttaat
     8761 acttgctgcg ttaccgtttc atagttaaat tgaatgggac actcaatttc ttcaagaata
     8821 aataattctt gtagagcatt tgcgtgaaat gcttccttaa ccatatgttc gccttcaact
     8881 agaaataaat ttgtcttatc tctttctttt ttttgaatca gtttggcata ttcttttatt
     8941 ttcttattgt ttattgattg aatcataaaa aaatattacc atacttcggc ttgaaaaaaa
     9001 acatggactt cgatataatt tgaactgtta aagcaatgag aagacatagt agctatttag
     9061 cgcttagata agagagtaaa ccaattggtg aaaggtttat atgagtaggg atagtgaatt
     9121 cacctttgag taagagttaa aacctcttcc gtttcatacg gttatcatgg tcaattaagt
     9181 gtgtctataa agacgaagca agatggtaac gcggcaaaaa gtcgttcttg cgtcgtcttt
     9241 ttttgttttt agggaaagga agaacaacat gaaaacaatc gaagagcttt tgcaggaagc
     9301 tacacaaaaa attcaagatg ttaaagattc atcttcattg aacgatttac gtgtcttgta
     9361 tttaggtaaa aaaggacccg ttcaagagtt gatgaagggt atgaaggatt tgtcgaaaga
     9421 agaaaaacct atctttggac aaaaggtcaa ccaattgaaa caagatcttt ctaagatgat
     9481 tgaagaaaag aaagaacaat tggcggcttt tgaaatgcag aaaaaaatcg aatctgaaaa
     9541 aatcgatatt acattacctg gtcgtaaacc agaattaggg aatgcgcacc cattaaattt
     9601 ggtacgccaa gaattggaag atttattcat tggtttaggt taccaagtta ttgaaggtga
     9661 tgatattgtg gatgatgaat attgttttga aagagccaat attcctaagg atcacccagc
     9721 acgtgatatg caggattctt tatatattga tccaaatcgt ttattgagaa cacatactac
     9781 ggccattcag atggttgtat tagaagaaag tgcaccaaat ttaccaatca aagttgtatg
     9841 tccaggtaag gtatatcgcc gtgatgatga tgatgcaacg cactctcacc aattcatgca
     9901 gatggaaggt ttagttattg gtgaaaaggt cactttagcg gacttaaagg gaactttaga
     9961 atttatggcc agaaagttat ttggtcaaga tcgccagatt cgttttagac ctagttattt
    10021 cccattcact gaaccaagtg tagaagtgga tgtttcttgt cacatttgta acggaaaagg
    10081 atgccctaca tgtaaaggga ctggatggat cgaaattcta ggtgctggag aagtacatcc
    10141 taacgtatta aaaatggctg gttatgatcc agaaaaatgt agtggttttg cgttcggtgt
    10201 aggtattgag cgtgttgcga tgttgaaata tggtattgat gatattcgtc atttctatac
    10261 aaatgataaa cgattcaatt ctacattcaa acgttatgag taggaggatt taaatatgcg
    10321 tatttcaaga aaatggttta atcaatttat ggatgtacaa gatctttcta ttgaagaaat
    10381 ggcaaaacgt attacagatg ctggttttga agtagaaggt attgaacatt tatcgcaagg
    10441 aacaaattta gtgattggtc acgttgaaac ttgtacggaa cacccagaca gtgaacattt
    10501 acactgtaca accgttaatg ttggaaatga agttgtcaat attgtgtgtg gtgcaccaaa
    10561 tgtggctgca ggacaaaatg taattgtcgc attaccagga gctgttttgc ctggtggaga
    10621 aatcaagaat ggtgtgattc gtggtgttga aagtaatggt atgatttgtt cattactaga
    10681 acttggggta gatccatcaa gtttaaatga tgatcaaaaa aatggtattg aagtattacc
    10741 agctgatgca ccagtaggaa atacggatcc attaggttac ttgggcttag atgatgaagt
    10801 attagatatt ggattaacac caaatcgtaa tgattgtcta gctgcattca atatggcaaa
    10861 agaagcagga gctattttaa atcgtgaagt taaacttcct gaatataatg gggctagtga
    10921 tgtgggtagc aaaacgcatc ttcatgttga aagcacaaca tcaaaatgtc cactattcta
    10981 tggcaaagtg atcaatcatt taacaatcaa agaaagtcca aaatggatga aagaattatt
    11041 ggcagctagt ggtgtaaaat caatcaataa tgttgttgat atttcaaata ttgttatgtt
    11101 agaaacaggg cagccaatgc acttctatga tgcaaagtca ttgcctagtc aaagtattgt
    11161 tgtagcagat ggatttgatg aagaatatac agcattagat ggtgtgactt ataaattaca
    11221 accagaagat attgtcatca caaaccaagg aaaaccaatc ggtattgccg gcgtcatggg
    11281 tggagatgat tctaaaattt tagatacgac agattcaatt attattgaat gtgcaatttt
    11341 cgatcatgta tcgattcgta atacagctcg tcgattaaat ttatcaacgg atgcgagtgt
    11401 tcgttaccaa aaaggtattg aaccattagc tagtaagaaa gcattggatc gtgctgttca
    11461 attgttgatt gaatatgccg atgcgacagg tattgaagaa acggttcaat atggacaagt
    11521 tccttatgaa ccaaaatcaa tttcttgtac tttaaaagct atcaacaatc gtttaggtac
    11581 agattttaaa ttagatgaag ttatcgatgt atttacacgc ttagactttg aacctgaagt
    11641 ggctgatgat ttgattacat gccacattcc aagttcaaga accgatatgg aaggaatggc
    11701 ggatttaagt gaagaagtca ttcgtatgtt aggatatgat cgtttgccta gtacacttcc
    11761 agttatgcct atgacagaag gtaaattaac gtataaacaa caattgactc gtcaggcacg
    11821 tcaattctta tgtgctcaag gtttacaaga ttgtttaaca tatactttaa ttagtacaga
    11881 aaagaaagag aatgcgattt tcaacacgga tgaagcgatt gaacttgcaa gtcctatgag
    11941 tgaagagcgt cgctatattc gtacaagtat tttgccaagt ttattagatg tagtttcata
    12001 taaccgtgct cgtaatatca aagatgtcaa tgtatttgaa ttgagtgatg tagctggtgt
    12061 aagtggtgct aaaatgcatt tagctatcgc catgagtggt aacttgcaac aaacgcgttg
    12121 gacaaatgat gtaactgtta gtgattttta tacattaaaa ggtttggttg aagctttctt
    12181 agaacagatt ggtattgccg ctcaaagaat ttcatttgta gaaaatgatt tggatacaac
    12241 acattttcat ccttatcgca gtgcaaaaat catgcttgga aaagattgtg ttggattatt
    12301 aggtgatatt catcctcaat atggtaagaa tattgtgatg gctgaacttg actttgatgc
    12361 gttgattgat gtgaaaaaat caaaaatcaa atttagtcca gtatcaaaat atccaagtgt
    12421 atctcgtgac ttggcattcg tattagatcg tgcaatgcca gtacaaaagg tagtggatac
    12481 aattaataag caaggtcgtt taaataaaga aatgattatc cgtgatgtag aagtatttga
    12541 tgtgtatgaa ggtgaacacg taagtaaaga tgaaaaatca gtagcgttat cgatcacatt
    12601 ccagtcggat tctcatacat taaaagatga agaaatcaac caagtgtttg aggcaatctt
    12661 agaacatatt caaaaagatt gtaatgcgac acttcgtagc taagagatgt ttttgacatc
    12721 tcttttcttt tgtctaacaa atcttgtaaa gggcttacaa aaagattaaa ataagacata
    12781 taaggagatt ttttctatgc aaaaatatat tttagcaatt gaccacggaa caacaagcac
    12841 acgtgccgta ttatttgata aggtagctaa cgtggtagaa attgcccaaa aagaaacgac
    12901 aatatcctac ccgcatccag gttgggtaga acaagatgcc aatgagattt ggcttgcgtg
    12961 tttagccgtg atgagcgagg tcattttaaa aagtaagatc agtccagaac aagtagctgc
    13021 cattggaata agtaatcagc gtgaaacaag tgttttatgg gatgcatcaa caggccttcc
    13081 tgtaagtccg gctattgtat ggcagtctaa acaaacaaag agtatttgcg atggttggaa
    13141 gaaaaagggt tatgaagata ttgtaaaggc gaaaacgggt cttcgtattg atccttattt
    13201 ttctgcaagt aagattcgtt ttatttttga tcagaatcca gatgtttatg aaaaggcgaa
    13261 aaaaggggaa gtcttatttg gcacgatgga tagttggatc gtatggaagt tgtctggttc
    13321 attgacacat attaccgatg tatcaaatgc gagtcgtacc ttgcttttta atattcatac
    13381 tttgtcttgg gatgatgagt tgcttgaaat gtttgatatt ccaaaatgta ttttacctca
    13441 agtaaaacct acatcctatg catattgtaa gacggctgcg tatcatttct ttggtcaaga
    13501 agtgccaatt tgttgtgttg taggagatca acaggcggct ttgtttggtc aaggttgctt
    13561 tggcgcaggt ggcattaaga atacatatgg tacaggtggc tttatgttga tgaatacggg
    13621 caagagaatt gtcgaatcta aaaatggttt attatcaact gtggcttggc aaattggtga
    13681 tcaggtaaat tatgctcttg aaggaagtat ttttgtttct ggtagtctaa tgaagtggct
    13741 tcgtgatcag atgcagttat ttgaaaatac aaaggatacg caggccatgg ctgaaagtgt
    13801 agagaataca aacggagtct atattgtgcc agcctttgtg ggtcttggag caccttattg
    13861 ggatgatgca tgtcaggcta gtattttagg cttaactttt ggagctaata aaaatcatat
    13921 tgtacgtgca gctttagaat ctatggcata tcaatcaaag gatgtattag aggctatgag
    13981 acaggatagt caaattgata ttacttctat gaaggttgat ggtggggctt gtgccaatga
    14041 cttcttgttg cagtttcaaa gtgatttact tcaaatgcct gtacaacgct ttaagacaaa
    14101 tgaactaacg gctttaggtg ctgcttatct tgcaggacta tcttgtggtt attggacaca
    14161 agatgagctt ggtgtagaac tttctaaaga gtttgtgcct gaaaagagta aagaagaaat
    14221 ggatagcttg tattcaaact ggaaaaaggc ggtaaaaact tgccagagtt acaaataaaa
    14281 gttagaatat tggcatggaa atttatacaa aagaagattt taaacaaatt tttatagatt
    14341 taggcattca aaaagggagc aaggttttat tggaagcaga tttgaatcgt tatagtggct
    14401 tggttggaaa tgttcaaatg ttgattgaaa ctttgcaaga attgattggg gatactggct
    14461 tgttgtgcat gccgtgtttc tcttattctt gtttggatcc tgcttgtctt gaaaactatt
    14521 atccttatga aaagtggaaa gaggttcgcg agaatttacc tggttttcat gtaatattta
    14581 cgccaagtga aatatataag gattgcacaa atttattttt acgttataaa gatgtttata
    14641 ggtcaaatca tcctgtatat agttttgcgt attggggtaa ttttgatgaa gatgttttaa
    14701 aggtggatgt caatgatgca ttaagttttc atggaagttt atctttttta aatgaaaaaa
    14761 atgcctgtaa ccttcttgtt ggtgtattac cagaaaagag tttaatggtg caggcacttt
    14821 caaatgagta tcaattggga aagacgattg tacaaagagc gtttcttcgt tcaaaaagac
    14881 aattgacaaa atcttttatt gttcaagttg tagatgaaga aatatctaga gatttactag
    14941 atggtttgac agttcttgaa agtgaatcga gtttagaaga tgtttatgta cttagaaaag
    15001 gggaataaag aattcgcaaa tcttttctaa gatagcttga tcttcaattg agaaattgtt
    15061 gaattcaaaa gaatcaatat ctaagatggc aacgagtttg tcatcttttt ttataggaat
    15121 acagatttcc gaattggaag cacaatcgca tgcaatgtga ccagcaaatt tatgtacgtc
    15181 ttcaattctt tgcgtttctt gcgttttagc acaagtacct acaacccctt ttccgtaagg
    15241 tatgcgtgta caggcaactt ttccttggaa aggacctaaa atgcattctt gatttttttc
    15301 atcacaaatg tagaagccta gccaattgat attttccatt tcatggaata aaagggcaat
    15361 caaattggat atgtttgcga tgtttgggag tgtttcatcc actaaatatt tgcattgttc
    15421 gattaatatt tcattcattt ttatcaccgt acttatttta ccaaaaaaac aatgaaatta
    15481 gtgacttttg tcattgaaaa aaaacttttt tttaagtact atataagtgt aaataaagct
    15541 ttttttaagg cttagggaat ttgggattga tactaatact tctgaaggaa taacagacag
    15601 taaaattgat agcgcgaatc atttttagtg tcaaaaacaa attttaacgg agagaagatt
    15661 ctagtaggtt tgacggagct gaatgaatct tcttttcttt ttttaaattt ttaaaaaaaa
    15721 ggggtttaca agaagggaag tatttggtaa tatagatgag cactgctgag gtgcacatga
    15781 tctttgaaaa ctgaacagga aataaaatac aaactaaaac gtcaatttcg aaaa
//
LOCUS       DBMDWL010000030        15614 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1278_length_15614_cov_2.782505, whole genome shotgun sequence.
ACCESSION   DBMDWL010000030 DBMDWL010000000
VERSION     DBMDWL010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 15614)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15614)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15614
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1278_length_15614_cov_2.782505"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="ACZPDN_03445"
     CDS             <1..457
                     /locus_tag="ACZPDN_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297709.1"
                     /translation="IEITGNTSYNFNEEDVYNLFYICRSIYDQSIYEEKSNSFGLVKS
                     SVLKVVKTLTDVEWLDLEELEEKEEIEMCEAEKRWLEVKSKEWEAEGIRKGIEQGIEQ
                     GIEQGIERGSEKKELEMYQKMMDKGFEIKAIASIFSVSEESIEKLLMKA"
     gene            601..1518
                     /locus_tag="ACZPDN_03450"
     CDS             601..1518
                     /locus_tag="ACZPDN_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR1297710.1"
                     /translation="MVFLLLVLGMVLLMKGADLFVDGSASVARKLKVPSVIIGLTIVA
                     LGTSLPEASVSITAGLMGSNEIAISNIVGSNIFNTLVVVGTSAVITPFLMDQDILNRD
                     LKINILVSILLSVFVLNGMLNRFEGIIFLVGLVLFILNLIRSAKKNRVEDEKVETLSG
                     IKCLIYIVIGVACIIWGGDITVDSAKQIAAMLGMSDTLIGLTVVSIGTSLPELVTSVV
                     AARKGESGLSLGNAIGSNIMNILFILGASSTIHPIAATSQNIIDTFVLIGVSLLIYGI
                     AKKGDTMTRKKGIFMILVYVIYMIYIIVR"
     gene            complement(1526..2134)
                     /locus_tag="ACZPDN_03455"
     CDS             complement(1526..2134)
                     /locus_tag="ACZPDN_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1297711.1"
                     /translation="MKSKIIENKKIKESNLLQAAFNLFTKEDITNVSVNDIVKSAGVA
                     KGTFYLYFKDKYQIRDILIQKEAHRLFEEAHKQLEKNDIRNFEDSVIFLINQVLIRLE
                     NNPLILKFVERNLSWGVFHAQLNTAIDNDSFNLIKEFNEIATANGYEFENSEVILYLI
                     VELTGSTCYNSILHSQPLPIEEYKPILFDSIRAILSQGKKKS"
     gene            2321..4402
                     /locus_tag="ACZPDN_03460"
     CDS             2321..4402
                     /locus_tag="ACZPDN_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1297712.1"
                     /translation="MDKMSKWIVKYRNLILAVAIILLIPSAIGYFNTNINYDLLSYLP
                     STSESMKTQKILGDDFNLSSVDFLVVNNKSDQEAAKIKEEINKIDGVEKCIWRDDVLD
                     ISVPKQAIPESIQDMLYSGNSTMMIVTFKEPTSSMRTMNAISKIKKYTKDDCYLGGIS
                     AISEDTKDQTEHDTPIYAGIAVVLCMVVLFLGLESTIAPVVFMLGMIFPIVYNFGTNV
                     FLGEISYITKALAVVLQLAVTLDYSIFLLHRYQEEKQKLPNEEAMAKAIKATFTSITS
                     SSITTIAGFVALCVMQLTLGRDIGIVMAKGVVLGVLSTIFILPSLLMFFDKTIEKYKH
                     KTILNELHRVPRFVIHHYKKILVAFVLIFIPFGYGQSHTNIYYDLISGMPGDFASIIG
                     TNQLKDKFDMTTTHFVLVDDKLTTNEIQNMCSEIKDLKGIHNVLAYEEFVGPGLASNF
                     TPSVVKDIFQNGGKKLIVVNSDYKAATDELNTQLDKMDTIIHKYDKKGMIGGEGSMTR
                     DLITTTNTDFAMVNVLSVIMIFIIVALTFKSFALPVILVLTIEFAITINMGIPFFTKT
                     TLPFIASMVIGTIQLGATVDYAILMTTRFKEELSHGKKVKEAALIAMEKSSVSIMTSG
                     FSFFAACIGVSFVAKMDLIKSLVILLARGALISVVSILLVLPSLLIFCHKFIEKTTKN
                     WPESNMEAKGE"
     gene            4405..6903
                     /locus_tag="ACZPDN_03465"
     CDS             4405..6903
                     /locus_tag="ACZPDN_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297713.1"
                     /translation="MNLSHTVKVVGSVVLSAVMAGSMMPVTVFAQNNDENPTEKTETV
                     YSVLNSDGSISDTIVSSWLHDEDGINNIKETLNLTDVKNIKSNEKPSKDGNTYTWNAK
                     GNDVYYEGTGTKQLPVSVKLRYELDGQEMSAKDMEGKSGHLKLTISFTNNYSEVKNIN
                     GKSIVIHPSYLAGGMLNMSTGNFTNVKCESGKIVNDGTNEMLAFANIPGLNETLRSAG
                     LDKVNNQLGISDDVTVEADVNNFDLGSIMVGMTNEIDLADELNGIGSVSELTDGIDQL
                     IEADDQLIDGSKQLYDGTTQLKEQAAPLTGSSDQVRQLSSGAIQLNDGVKALQTGLIA
                     YTNGVDTLAAGSQQLYGIPQGVSQIQTGVSGNLGQGKTNLLDGATQLNEGLKQLKAQV
                     NAITPGQLETMQNQVSTSMKTLEGMKTLLGNDVQTLTTLQTTLGEAVKTLDTLANSKT
                     GELTQKIGAVMKDVATLKAQIDNDKTIIDSHNQDITKKVKDINDQIDTINSQINTAVN
                     TANGNIDTAYSNANSTIESAAQKAEAEGNTDLANQIRSCKLQAAQKIDAPSVENGMQL
                     SHVTLGELKSFDEIKTNDFALHIQDLNDEISKIEKTLAGMNKQLNDAKILILGENLDG
                     TAGLAGDVQNAINTLGQMNSMLDTYTADDSTMNFKKLVTDLQAAAKELSAGSEGILGG
                     VQQVNAGLTLLQKKSEAGITQVAEGSKTLSSNSATLNGGASALSDATGTLAGQSGTFN
                     EMADGLDTLGKAFETLNDGAKQLYEGNEQFKSEGLDQLKEKVDLGVGELETLQSVMNE
                     IKAMNKEYASYSGAPEGATVTSRYVFRTKEESSK"
     gene            6997..7149
                     /gene="scfA"
                     /locus_tag="ACZPDN_03470"
     CDS             6997..7149
                     /gene="scfA"
                     /locus_tag="ACZPDN_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HIR1297714.1"
                     /translation="MKHINTVDTRNLCESAKNGGCGECQTSCQSACKTSCGIANQACE
                     NNKESK"
     gene            7230..8624
                     /gene="scfB"
                     /locus_tag="ACZPDN_03475"
     CDS             7230..8624
                     /gene="scfB"
                     /locus_tag="ACZPDN_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1297715.1"
                     /translation="MIHQYKMFDQNIVLDICSGSIHVVDEIAYDMIAEFLDKDKNTLV
                     LETKEKYPEVETSELEECYDQILELKEQGRLFSEDTYEPMAGQLKAKTSGVIKALCLH
                     IAHTCNLNCSYCFASQGKYHGDRALMSFETGKRALDFLVENSGTRRNLEVDFFGGEPL
                     MNFDVVKQLVAYARSIEKEAGKNFRFTLTTNGMLIDDDVIEFANKEMSNVVLSLDGRK
                     EVHDRYRVDYSGKGSFDTIVPKFQKLVKAREGKNYYMRGTFTHANPDFLKDVQQMLDL
                     GFRELSMEPVVAKSDDPAALTKADREIVMKQYEDLAKLMLEYDDKKDPFTFYHYMIDL
                     KGGPCIYKRISGCGSGTEYMAVTPWGDLYPCHQFVGEEAFKLGDIYTGVTNKKIQDEF
                     ASCNVYARKECADCWARLYCSGGCAANAYHATGSVKGVYKEGCELFCKRLECALAVAI
                     IRDMKENSHEDADC"
     gene            8605..9933
                     /locus_tag="ACZPDN_03480"
     CDS             8605..9933
                     /locus_tag="ACZPDN_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013444.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1297716.1"
                     /translation="MKTPIVSFLKSYQEKSPVRMHMPGHKGAGILGFEGMDLTEIYGA
                     DELFAAEGIIKKSEQNASSLFGCPTYYSTQGSTLCIQTMCTILCQDAKSKGKKPKILA
                     GRNAHRSFIHAAALLDFDIAWLYGNSDYLSCTISIETLEKEILENNPTAVYLTNPDYL
                     GNLLDIKSLASICKKHDVLLAIDNAHGAYLRFLEPSLHPIDLGADLCCDSAHKTLPVL
                     TGGAYLHLSDSLNQVWKNDVKHFMEYFSSTSPSYLIMASLDATNEVLETTFKKSLSEC
                     IQRVDVLKNSLIQHGYTILSGEPMKITISTKDFGYTGNEIANLLMECDIYPEFYDSDY
                     IVLMPSPYNTKDDLKRLETCLCKIERKSVVFNSFPKLERARKVMNVRQALFAQSTMIE
                     VSKSLGQVCSSVSVSCPPAILPVIPGEVISEFSIEVMKYYGIETVRVVKE"
     gene            10022..10945
                     /locus_tag="ACZPDN_03485"
     CDS             10022..10945
                     /locus_tag="ACZPDN_03485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR1297717.1"
                     /translation="MFDKLFEPIQIRGMELRNRVVMSAMGTHESAESEDGKSVTDKLI
                     AYHVARAKGGNGLNTIEVTSVDAPSAPFGFLSIAEDKYIPGFKKLNDAVHEAGGKTCI
                     QLWQGGLAVASDQTAQILVPNDLPLSPQYTVPAITNERLLDIIDKFGQAARRAVEAGF
                     DCLEFHCAHNYLPHSMLSGGINHRSDEWGGSFENRKKFPLECIKSIRANMPEDMPLFM
                     RVDCHDDMLEGGLTVEEVIKFCRDAKEFGVDVLNISRGNILTAATLYEVAPVDIENGF
                     NVEDAARIRKETGMLTMPCGRINTPEFAKRF"
     gene            10972..11952
                     /locus_tag="ACZPDN_03490"
     CDS             10972..11952
                     /locus_tag="ACZPDN_03490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1297718.1"
                     /translation="MARAQLADANFCNKAKDGKVNQIRYCIGCNQGCYDSFCASLYNP
                     AIKHITCMRNPGLLEEETLGLKPGSASKKVVILGGGIAGMEAALDLKETGNTPILFEK
                     SESLGGQFLLAGAISAKKDFKNAVDKMILDVQESGIEVHLNTVVTKEMLEEIKPDALI
                     VAIGSSPFVLPIEGSEKAIVAHDVLSEKVEVPGENVVIIGGGLVGFETAEFLAARQKK
                     VTVLEMKEKALQDLGPLRQITAQFAMAQMPITIETSATVERIEDHAVVASGKEYPYDS
                     VIMAVGSKANDTSMYTDLGIPTYIIGDCKKAGVALDAFKDAYHAVLEINK"
     gene            complement(11957..12697)
                     /locus_tag="ACZPDN_03495"
     CDS             complement(11957..12697)
                     /locus_tag="ACZPDN_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1297719.1"
                     /translation="MMNPIIQSLFDRKSCRIFKDKEIFQEERKLILEACMQAPSAGNQ
                     QMYTIIDVQDKKLQETLSKTCDNQLFINQAKMVLIFCADFQKWYDLFNHENCNPRKPG
                     VGDLLLATTDATIAAQNAVVAAESLGIGSCYIGDILEQHDLHKELLNLSEYVVPVCML
                     VLGYPAEDHFKRQKPQRCKLEDIICVDKYHRKNKLELKNMFEHKAGNKALNEWATAFC
                     NRKYNSDFSKEMTNSVQKYIDQFKSEAD"
     gene            12765..14420
                     /locus_tag="ACZPDN_03500"
     CDS             12765..14420
                     /locus_tag="ACZPDN_03500"
                     /inference="COORDINATES: protein motif:HMM:NF017927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR1297720.1"
                     /translation="MKKNAHKNVKTHYATYLVLCLAAVLMGSEFSSTLSLLKQDNAKS
                     VSGLLMHSSFVKSMTSLLPEGVFGTTNGVFAHVVNGIASGSFVKTLFLGLSTIVHSKD
                     IASICFVVIGLCISVFYRVYIVNVLPVINRRLFLEGRVYAKLPLDRLVYLMRIRKQMH
                     VAFVKLVKSIILTIMNFTVVGGVYFYYVYFLVDYILAENPTISLKDALSLSRNMMKGH
                     KFECFKWQFSMAGWYILDVCTFGISAIFYSNMYRMSVMCEFYTLRRKEFQSAILNDTY
                     LFEKPSSALMRNTYSDVIAALNAKNPMENAYTGIKKFLCDNFGIIFHLSSKEKQYERY
                     TYNKMEAETMITEVYLLCYPVRLSPIEEAYKKSNLRVLHPNRNYTVTSILVCFFFMCF
                     VGWIWEVSLSMISYGRFVNRGVLHGPWIPIYGFGCVLILLLLKRFRMRPKVEFSMAVL
                     LCGCIEYFTGFFLELTHNGQKWWDYTGYFLNLHGRICAEGLLVFGVGGMAFVYVIAPL
                     IDNWVKEHLNKRLSTACLVLLLLFGADVVYSHFEPNVGEGVTG"
     gene            14417..15127
                     /locus_tag="ACZPDN_03505"
     CDS             14417..15127
                     /locus_tag="ACZPDN_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1297721.1"
                     /translation="MIKKLNGNQIKMIAIIAMFMDHFATTLYSNYEKFWWLLLIHLIG
                     RVAAPIMWFMVVEGYTHTHSLKKYLERLLIFSIIAHFAYNFCFGISLIPFKESFLNQT
                     SIIWPLFLSVIGLWVEDQEWKDAKKIGCILVLCLLAFPSDWSCFPILCTNHIYKNQGN
                     LKKQVLGMVLYISMYVFVYCLCIDVVYGLLQFGIVLVYPIMSLYDGTRGKSTFMKWFF
                     YWFYVGHLILCGLIRVMM"
     gene            15155..>15614
                     /locus_tag="ACZPDN_03510"
     CDS             15155..>15614
                     /locus_tag="ACZPDN_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297722.1"
                     /translation="MHEISRKILNHYQIRKTKKQKEEFRKFLVDELKQYGYCPIVQNK
                     GINNNIVIGNMDTARIFCTAHYDTQAVLPFPNLIVPNNLFGLILSQFAIIICVLILIF
                     FMNIVLIKLFVWTGLFKNIELNIASTISWLIIMIWMLFGKANKHTANDNTS"
BASE COUNT         5197 a         2144 c         3145 g         5128 t
ORIGIN      
        1 gattgaaatc acaggaaata cttcatataa ttttaatgaa gaagacgtgt ataatctatt
       61 ctacatatgt cgaagtattt atgatcagtc tatttatgaa gaaaagagta atagttttgg
      121 acttgtgaag agcagtgtgt taaaagtagt caagacatta acggatgtag agtggttgga
      181 tttagaagaa ttagaggaaa aggaggagat tgaaatgtgt gaagctgaaa agagatggct
      241 tgaagtaaag tcaaaagaat gggaagctga aggaataaga aaaggaatcg aacaaggaat
      301 tgaacaagga atcgaacaag gaattgaacg aggtagtgag aaaaaagagc ttgaaatgta
      361 tcaaaaaatg atggacaagg gatttgaaat taaagctatc gcatctatct tctcagtaag
      421 tgaagagtcg attgaaaagc tgttgatgaa agcttaggcc aagatgaaaa gagatggctt
      481 cgaaaaagaa gatacgttct tttgttcaat gtttgtaaca atggatagga ggagatgaaa
      541 aatctcttct tttctttttc gtctgtgata taataaggtg actttgaaag gattgaaaaa
      601 atggtatttc ttttattagt attaggtatg gttcttttaa tgaagggtgc agacttgttt
      661 gtggatggca gtgcaagtgt ggctcgtaaa ttaaaagtgc cttcagttat tattggtttg
      721 acaattgttg cgctaggaac aagcttacca gaagcaagtg taagtattac ggctggttta
      781 atgggcagta atgaaattgc gatttcaaat attgtaggtt ctaatatttt caacacgttg
      841 gttgtggttg gaacaagtgc agtcattact ccgtttttaa tggatcagga tattttgaat
      901 cgtgatttaa aaatcaatat tttagtgagt attttacttt ctgtttttgt attaaatgga
      961 atgttgaatc gctttgaagg tattatcttc ttagtcggtt tagtattgtt tattttaaat
     1021 ttgattcgta gtgctaaaaa gaatcgtgtt gaggatgaga aagttgaaac tttatcggga
     1081 atcaaatgcc tgatttatat cgtgattggt gttgcgtgta ttatttgggg tggagatatc
     1141 actgtagata gtgcaaaaca gattgcagct atgcttggta tgagtgatac tttgattggt
     1201 ttaactgtcg tatctattgg aacttcttta cctgagttag taacaagtgt tgttgcggca
     1261 agaaaagggg agagtggatt atctttagga aatgcgattg gaagtaacat tatgaatatt
     1321 ttatttattt tgggtgcttc aagtacaatt catccaattg cggctacttc acagaatatt
     1381 attgatactt ttgtcttgat tggtgtttct ctattaattt atggtattgc caaaaaggga
     1441 gatacgatga caagaaaaaa ggggatcttt atgatcctcg tatatgtcat ttatatgatt
     1501 tatattattg tacgctaggc ttgtcctagc tttttttctt tccttgggaa aggatagctc
     1561 gaatagaatc gaaaagaatg ggtttatatt cttcaatcgg caggggttga ctatgaagaa
     1621 tactgttata gcaagtactt cctgtaagtt caactattaa gtagagtatg acttctgaat
     1681 tttcaaattc atatccatta gcggttgcga tttcgttaaa ttcttttata agattgaaac
     1741 tatcattatc aatagccgta tttaactgag catggaacac gccccaagat aggttccgtt
     1801 caacgaattt taaaatcaat ggattgtttt ctaatcgaat caatacttga ttgatcaaga
     1861 agataacaga gtcttcaaaa ttacgaatat cgtttttttc taattgtttg tgggcttctt
     1921 caaaaagacg atgtgcttct ttttgaatta gaatatcgcg aatttggtat ttatctttga
     1981 aataaagata gaaagttcct tttgcgacgc ctgcactctt tacaatatca tttacagaga
     2041 cattcgtaat gtcttctttt gtgaatagat taaaggcagc ctgtaagagg ttgctttctt
     2101 ttattttctt attttcgatt atcttacttt tcatgcatat caccatccat attataatca
     2161 ataatttata aaatacaaat aaaaatgacc gattgaacat attaaaacaa ttgtatattg
     2221 ggaattttaa aaaaataaaa caaaattatt gactagtggt cagtttggta ctatactctt
     2281 cacgtagtaa aggaaaggat agaaaaaaag gtgattcgat atggataaga tgtcaaaatg
     2341 gatagttaaa tatcgtaatt tgattttggc cgtagccatc atactattaa ttccaagtgc
     2401 aattggttat ttcaatacaa atattaatta cgatttatta tcttatttac cttcgacttc
     2461 agaatctatg aaaacacaaa agattcttgg agatgatttt aacttatcga gtgtagactt
     2521 cttggttgtc aacaacaaat cagatcaaga ggccgcaaaa attaaggaag agatcaataa
     2581 aattgatgga gttgaaaaat gtatttggag agatgatgta ttagatattt cagttccaaa
     2641 acaggcaatt ccagaaagca ttcaagatat gctttattct ggcaacagta caatgatgat
     2701 tgttacattt aaagagccaa catcgtcaat gcgtacaatg aatgcgatta gtaaaataaa
     2761 aaaatataca aaagatgatt gttacttagg tggtatttca gccatcagtg aagatactaa
     2821 agatcaaaca gaacatgata caccaattta tgcaggcatt gccgttgtat tatgtatggt
     2881 cgtattgttc ttaggattag agagtacaat tgctccagtt gtatttatgt taggaatgat
     2941 tttcccaatc gtttataact ttggtacaaa cgttttctta ggtgaaatta gttatatcac
     3001 aaaggctttg gctgttgtat tacagttagc tgtaactttg gattattcca tcttcttatt
     3061 acatagatat caagaagaaa aacaaaaact tccaaatgaa gaagctatgg caaaggctat
     3121 caaagctacg tttacttcaa ttacttcaag ttcaatcaca acaatcgcag gttttgttgc
     3181 cttgtgtgta atgcagttaa cactaggtag agatattggt attgtcatgg caaaaggtgt
     3241 tgttcttggt gtattatcaa ccatctttat tttgccaagc ttgttgatgt tctttgataa
     3301 aacgattgaa aagtacaaac ataaaacaat tttgaatgag ttacatagag tacctcgttt
     3361 tgtgattcat cactataaaa agattcttgt tgccttcgtg ttgattttca ttccatttgg
     3421 atatggacag tcacatacaa atatctatta tgatttgatt tctggtatgc caggtgattt
     3481 tgcgagtatc attggtacaa atcagttaaa agataaattt gatatgacaa caacacattt
     3541 cgttttagtg gatgacaaat taacgacaaa cgaaattcag aatatgtgtt ctgaaatcaa
     3601 agatttaaaa ggaattcata acgtactagc ttatgaagaa tttgtgggtc caggacttgc
     3661 atccaacttt actccaagtg tagtaaagga tattttccaa aatggtggaa agaaattaat
     3721 tgttgtgaac agtgattata aagctgcaac cgatgaatta aatacacagc tagataaaat
     3781 ggataccatc attcataaat atgataagaa gggtatgatt ggtggagaag gaagtatgac
     3841 acgtgatttg atcacaacaa caaatacaga ctttgcgatg gttaacgtat tatccgttat
     3901 tatgatcttt atcatcgttg ccttaacatt taaatcattt gcacttccag tcattttagt
     3961 actaacaatt gaatttgcga ttacgatcaa tatgggtatt ccattcttta caaaaactac
     4021 acttccattt atcgcaagta tggttattgg aaccattcaa ttgggagcta ccgttgacta
     4081 cgctatccta atgacaacgc gttttaaaga agaattatcg catggtaaga aggttaagga
     4141 agcggcttta attgcgatgg aaaaatcgag tgtgtccatt atgacaagtg gattctcatt
     4201 ctttgccgca tgtatcggtg taagttttgt agctaaaatg gatttaatta aatcattagt
     4261 cattttatta gcacgtggtg cattaatttc agtcgtaagt attttattag tattaccttc
     4321 attgttgatc ttctgccaca agtttattga aaaaacgaca aagaattggc cagaatcaaa
     4381 catggaagcg aaaggagaat aaatatgaat ttaagtcata cagtaaaagt agttggtagt
     4441 gtagtgttga gtgcagttat ggcaggaagt atgatgcctg tgactgtatt tgcgcaaaat
     4501 aatgatgaaa atcccacaga aaaaacagaa actgtttatt ctgtattaaa ctcagatggt
     4561 tccatcagcg atacaatcgt atctagctgg ttacatgatg aggatggaat taataatatt
     4621 aaagaaactt taaacttaac agatgttaag aacattaaat caaatgaaaa accttcaaaa
     4681 gatggaaata catatacatg gaatgcgaaa ggaaatgatg tgtattatga aggaacagga
     4741 acaaaacaac ttcctgtgtc tgttaagctt cgttatgaat tagatggtca ggaaatgtct
     4801 gcaaaagata tggaaggtaa gtctggtcat ttgaaattaa ctatttcttt cacaaataac
     4861 tattcagaag ttaaaaatat caatggcaaa tccattgtga ttcaccctag ttatttagct
     4921 ggtggtatgt tgaatatgag tactggtaac tttacaaatg tgaagtgtga atctggtaag
     4981 attgtaaatg atggaacaaa tgaaatgttg gcatttgcga atattccagg cttgaatgaa
     5041 acattaagat ctgctggttt agataaagta aacaatcaat taggaatcag tgatgatgta
     5101 acagtagaag cggatgtcaa taattttgat ttaggttcta tcatggttgg tatgacaaat
     5161 gaaattgatt tagcagatga attaaatggt attggttctg tatctgaatt aacagatgga
     5221 attgatcaat taattgaagc cgatgatcaa ttgatcgatg gatctaaaca attgtatgat
     5281 ggaacaacac aattaaaaga acaagctgct ccattaacag gctcatctga tcaagtaaga
     5341 caattatcta gtggtgctat ccaattaaat gatggtgtta aagccttaca aacaggatta
     5401 atagcttata caaatggtgt agatactttg gcagcaggaa gccaacaatt atatggaatt
     5461 cctcaaggtg tatctcaaat tcaaactggc gtttcaggta acttaggtca aggaaaaaca
     5521 aatctattgg atggtgcaac ccaattaaat gaaggcttaa aacaattaaa agcacaagta
     5581 aatgcgatta ctccaggtca attagagact atgcaaaatc aagtttcgac atctatgaaa
     5641 acattagaag gtatgaagac tctgttaggt aatgatgtac agacattgac aactttacaa
     5701 actactttag gcgaggctgt aaagacatta gatactttag ctaattcaaa aacaggtgaa
     5761 ttaacacaaa aaattggcgc tgtcatgaag gatgttgcta ctttaaaagc acaaatcgat
     5821 aatgataaaa caatcattga ctcacataat caagatatca caaaaaaagt aaaagatatt
     5881 aatgatcaaa ttgatacaat caattcacaa atcaatacag ctgtaaatac ggcaaatggt
     5941 aatattgata ctgcctattc aaacgcaaat agtacaattg agagtgcagc acaaaaagcg
     6001 gaagcagagg gaaatacgga tttagctaat caaattcgct catgtaaatt acaagctgct
     6061 caaaagattg atgcaccaag tgttgaaaac ggaatgcagt taagtcacgt tactttaggc
     6121 gaattaaagt catttgatga aattaagaca aatgattttg cattacacat tcaagatttg
     6181 aatgatgaaa ttagtaaaat tgaaaaaact ttagctggaa tgaataaaca gttaaatgac
     6241 gcaaagatac ttatcttggg tgaaaactta gatggaaccg ctggattagc tggagatgtt
     6301 caaaatgcaa ttaatacatt aggtcaaatg aattctatgt tggatacata tacagcagat
     6361 gattcaacaa tgaatttcaa aaagttagta actgatttac aagctgctgc taaggaattg
     6421 tctgcaggtt ctgaaggtat tctaggtggt gttcaacaag tcaatgctgg attgacacta
     6481 cttcaaaaga aatctgaagc aggtattact caagtagctg agggaagtaa aacattgtct
     6541 tcaaatagtg ctacattaaa tggtggtgct agtgcattat cagatgcaac tggaacactt
     6601 gctggacaaa gtggcacatt caatgaaatg gctgatggat tagatacatt aggtaaggca
     6661 tttgaaacat tgaatgatgg tgctaaacaa ttgtatgagg gtaatgaaca attcaagtct
     6721 gaaggattgg atcaattaaa agaaaaggtt gatttaggtg ttggagaact tgaaacatta
     6781 caaagcgtaa tgaatgaaat caaagctatg aacaaagaat atgcttctta ctctggtgct
     6841 ccagaaggtg ctacagtaac gagtcgttac gtattccgta ctaaggaaga aagttctaaa
     6901 tagtttataa aggtattctt cggaatgcct ttttaatttg atattatttg tttggcgtgt
     6961 taaaataaaa gagaatttat atgggggtat ataatcatga aacatattaa tacagtagat
     7021 actcgtaact tgtgtgaaag cgcaaaaaat ggtggatgtg gagaatgcca aacttcttgc
     7081 caatcagctt gtaaaactag ctgcggaatt gccaaccaag cttgcgaaaa taataaagaa
     7141 agtaagtaat tcagaaatgc cgaagtgatt cggcattttt aatgccgcct ttaaggcagc
     7201 attttttaaa tatgagggag gaaaaataca tgattcatca atataaaatg tttgatcaga
     7261 atattgtatt ggatatttgt tcaggaagca ttcatgtggt agatgaaatt gcctatgata
     7321 tgattgctga atttttagat aaagataaaa atactttggt tctagaaacg aaagaaaaat
     7381 atcctgaagt agaaacatct gaattagaag aatgctacga tcaaatctta gaattaaaag
     7441 aacaaggccg tttatttagt gaagatacat atgagccaat ggcaggccaa ttaaaagcta
     7501 agacaagtgg cgttattaaa gctttatgtc tacatattgc ccatacttgt aacttgaatt
     7561 gttcttattg ttttgcaagc caaggaaaat atcatggaga tcgcgcatta atgagttttg
     7621 aaacaggaaa acgcgctctt gatttcttag tggaaaattc aggaacacgc agaaacttag
     7681 aagtggattt ctttggtgga gaaccactaa tgaattttga tgtagttaaa caattagttg
     7741 cctatgctcg tagcattgaa aaagaagctg gaaagaattt ccgttttaca ttaacgacaa
     7801 atggtatgtt gattgatgat gacgtaattg aatttgcgaa caaagaaatg agcaatgttg
     7861 tattaagctt agatggacgt aaagaagtgc atgaccgtta tcgtgtggat tattctggta
     7921 agggaagttt tgatacgatt gtccctaagt tccaaaagct agtaaaggct cgtgaaggta
     7981 aaaattacta tatgcgtggt acttttacac atgccaatcc agacttctta aaagatgtgc
     8041 aacaaatgtt agatttagga tttagagaac taagtatgga gcctgtggtt gctaaatcgg
     8101 atgatcctgc agcactaaca aaggcagatc gtgaaattgt tatgaaacaa tatgaagatt
     8161 tagcgaaatt gatgttggaa tatgacgata agaaagatcc atttactttc tatcactata
     8221 tgattgattt aaagggcgga ccatgtattt ataaacgtat ttcaggatgt ggttcaggca
     8281 ctgaatatat ggccgttaca ccatggggag atttgtaccc ttgtcaccag tttgtaggag
     8341 aagaagcctt taaattagga gacatttata cgggtgtcac aaacaaaaag attcaggatg
     8401 aatttgcatc atgtaacgta tatgcacgta aagaatgtgc agattgctgg gcaagacttt
     8461 attgttctgg aggatgtgca gccaatgcat atcatgcgac aggatctgtc aaaggtgtat
     8521 ataaagaagg ttgtgaatta ttctgtaagc gattagaatg cgcacttgcc gtagccatta
     8581 ttcgtgatat gaaagaaaat agtcatgaag acgccgattg ttagttttct aaaatcgtat
     8641 caagaaaaat cacctgtaag aatgcatatg ccaggccata aaggtgctgg cattcttggt
     8701 tttgaaggaa tggatcttac agaaatttat ggagccgatg agttgtttgc ggcagagggt
     8761 atcattaaga aaagtgaaca aaatgcttcc tctttatttg gctgtccaac ctattattca
     8821 acgcaaggct ctactttatg catccaaact atgtgcacga tattatgcca agatgctaaa
     8881 agtaaaggca agaagccgaa gattcttgca ggaagaaatg cacatcgaag ctttattcac
     8941 gctgctgcac ttttagattt tgacattgca tggctatatg gaaatagtga ttatttatct
     9001 tgtacaatta gtatagaaac tctggaaaag gaaattcttg aaaacaatcc aacggctgta
     9061 tatctgacaa atccagacta tttaggaaac cttttggata ttaaatcttt agcttcgata
     9121 tgtaaaaagc atgatgtatt acttgccata gacaatgccc atggtgcata tcttcgtttt
     9181 ttagaaccat ctttacatcc tattgatttg ggagctgatc tatgttgtga ttcggctcat
     9241 aaaacactac ctgtacttac cggtggagct tatcttcact taagtgattc tttgaatcaa
     9301 gtatggaaaa atgacgttaa acattttatg gaatattttt cttctacaag tccatcctat
     9361 ttaattatgg catctttaga tgcgacaaat gaggtgttgg agactacgtt taaaaaatcc
     9421 ttgtctgaat gtattcaaag agttgatgta ttaaagaatt ctttgattca acatggatat
     9481 acgattttat ctggtgaacc tatgaagatt acgatttcaa cgaaagattt tggctatact
     9541 ggaaatgaaa tcgcaaatct tttaatggaa tgtgatatct atcctgaatt ctatgattca
     9601 gactatattg tgttaatgcc atctccatat aatacgaagg atgacttaaa acgattagag
     9661 acttgtttat gtaaaattga acggaaatct gttgtattca attcatttcc taagctagaa
     9721 cgtgcaagaa aagtaatgaa tgtacgccaa gctttatttg cgcaaagtac aatgatagaa
     9781 gtttctaaat ctttaggaca agtatgctca agtgtttctg tatcttgtcc tccagcgatt
     9841 cttccagtga ttccaggaga agtgattagt gagttttcta ttgaagtcat gaaatattat
     9901 ggtattgaaa ctgtacgagt tgtcaaagaa tgacagctct ttttcttttg tatatgtgaa
     9961 accgctaaca attgaagtat aataaaataa gaagtaaaca tgcgaaaagg aggagaatca
    10021 tatgtttgac aagttgtttg aacccataca gattcgtggt atggaattaa gaaatcgtgt
    10081 tgtcatgtct gcgatgggaa cgcatgaaag tgcggaaagt gaagatggaa agagtgttac
    10141 agataagttg atcgcgtatc atgtagctcg tgcaaaaggt ggtaacggat taaatacaat
    10201 tgaagttaca agtgtggatg ccccgagtgc accttttgga ttcttgtcga ttgccgaaga
    10261 caagtacatt ccgggattta aaaaattaaa tgatgccgtg catgaagccg gaggaaaaac
    10321 ttgcattcaa ttatggcaag gaggattagc agtagcttca gatcaaacag ctcagatttt
    10381 agtacccaat gacttaccat taagtccaca atatactgtg ccagctatca caaatgaaag
    10441 attactcgat ataatagata agtttggaca agctgctaga cgtgcagtag aagctggatt
    10501 tgattgttta gaattccatt gtgcacataa ctatttacca cactctatgc tttcaggagg
    10561 aattaaccat cgaagtgatg agtggggtgg atcttttgaa aatcgtaaaa aattcccgtt
    10621 ggaatgtatt aaatcgattc gtgccaatat gccagaggac atgccactat tcatgcgtgt
    10681 ggattgtcat gacgacatgt tagaaggtgg attaacggtt gaagaagtga ttaaattttg
    10741 tagggatgcc aaagaattcg gtgtggatgt tttaaatatt agtcgtggaa atattttaac
    10801 ggcagctaca ctatatgaag tggctccagt ggatattgaa aatggattta acgtagaaga
    10861 tgctgcacgt attcgaaaag aaacaggtat gttgacaatg ccatgtggac gtatcaatac
    10921 gcctgaattt gcgaaaagat tttagaagat gacaaggccg atttagttgt catggcacgt
    10981 gctcaacttg ccgatgccaa cttctgtaat aaggctaaag atggtaaagt caatcaaatt
    11041 cgctattgta ttggatgtaa ccaaggctgc tatgactcat tttgtgcatc tttatataat
    11101 ccagctatta aacacattac ttgtatgcga aatcctggct tattagaaga ggaaacatta
    11161 ggtttaaaac ctggatctgc ttctaaaaaa gttgtcatct taggtggtgg aattgcaggt
    11221 atggaagcag ctctagattt aaaagaaaca ggtaatacac caattttatt tgaaaagagt
    11281 gaaagtcttg gtggacaatt tttattggcc ggagcgattt cagctaaaaa agactttaag
    11341 aatgcggtag ataaaatgat tttggatgtt caagaatctg gaattgaggt gcacttaaat
    11401 actgttgtta ctaaggaaat gctagaagaa attaagccag atgcactcat tgttgcgatt
    11461 ggatctagtc catttgtact tccaattgaa ggtagtgaaa aagccattgt tgcacatgat
    11521 gtattgagtg aaaaagtaga agtgccaggt gaaaacgtag tcattattgg tgggggtctt
    11581 gttggttttg aaacagctga atttttggct gcaagacaaa agaaagttac tgttttagaa
    11641 atgaaagaaa aggctttaca agatttaggt ccattgcgtc aaattacagc gcagtttgcg
    11701 atggcacaaa tgccaatcac aattgaaaca agtgctactg tagaacgaat tgaggatcat
    11761 gcagttgttg ccagtggcaa agaatatcca tatgatagtg tcattatggc tgttggatca
    11821 aaggcaaacg atacaagtat gtatacagat ttaggtattc ctacatacat cattggtgat
    11881 tgtaaaaaag caggcgttgc tttagatgca tttaaagatg cttatcatgc cgtattagaa
    11941 attaataagt gaagtcttaa tcggcttcac ttttaaattg gtcaatatat ttttgtacag
    12001 aatttgtcat ttcttttgaa aaatccgaat tgtattttcg attacagaaa gctgttgccc
    12061 attcatttaa tgctttattt ccagctttat gttcaaacat attttttaat tccagtttgt
    12121 tttttctatg atatttatca acacaaatta tatcctctaa tttacagcgc tgtggttttt
    12181 gtcttttaaa atgatcctct gcaggatatc caagtactaa catgcataca ggcactacat
    12241 attcacttag atttaataat tctttatgta gatcatgctg ctctaaaata tcccctatgt
    12301 aacaagatcc aatccctaaa ctttcggcag ctacaactgc attttgtgcg gcaatggtcg
    12361 catccgttgt ggccaataat aaatcgccta cgcctggttt tcttgggttg cagttttcat
    12421 ggttaaataa atcataccat ttttgaaagt cagcacagaa aattaaaacc attttagctt
    12481 gattgatgaa gagttggttg tcacaagtct tagatagtgt ttcttgcagt ttcttatctt
    12541 gtacatcaat aatagtatac atttgttgat ttcctgcact cggtgcctgc atgcaagctt
    12601 ctaatatcaa ttttctttct tcttggaaaa tttctttatc tttaaaaata cgacaagatt
    12661 tgcggtcaaa taaactttga ataattggat tcatcataaa tcccctttct tttgaaaata
    12721 ttttactata gatatggtat tatttaagta taaaacttaa agaaattaag aaaaatgcac
    12781 ataaaaatgt aaaaacacat tatgcgacct atttagtgtt atgtcttgcg gcagtactta
    12841 tgggaagtga atttagttcg actttatccc ttttaaaaca agataatgcc aagagtgttt
    12901 ccggcctatt aatgcactct tcttttgtga agtcgatgac atctttactt cctgaaggcg
    12961 tctttggtac gaccaacggt gtatttgcac atgtcgtaaa tggaattgct tcaggatcct
    13021 ttgtgaagac actattttta ggcttatcca ccattgttca ttcaaaagat attgcgagta
    13081 tttgttttgt cgtaataggt ttatgcatat ctgtgttcta tagagtatat attgtgaatg
    13141 tattgccggt tattaaccgc agactctttt tggaaggaag agtttacgca aaacttccac
    13201 ttgatcgatt ggtttattta atgcgaattc gaaagcagat gcatgttgcg tttgtgaaac
    13261 ttgttaaatc tattatcttg acaattatga actttactgt tgtaggaggc gtttactttt
    13321 attatgtata tttcttagtg gattatatct tagccgaaaa tccgacgata tctttaaaag
    13381 atgcactttc tttatctcga aatatgatga agggacataa gtttgaatgt tttaaatggc
    13441 aattttcaat ggctggatgg tacattttgg atgtttgtac atttgggatt agtgctatat
    13501 tttattcaaa tatgtatcgt atgtcagtaa tgtgtgaatt ttatacatta agaagaaaag
    13561 aatttcaaag tgctatctta aatgatacgt atttatttga aaaaccaagt agtgctttga
    13621 tgcgtaatac gtattcggat gtaattgcgg cattgaatgc gaaaaatcca atggagaatg
    13681 cgtatacggg tattaaaaag ttcttgtgtg ataattttgg gattattttt catttgagtt
    13741 ctaaagaaaa acaatatgaa agatatacct ataataaaat ggaagctgaa actatgatta
    13801 ccgaagtgta tttattatgt tatccagtgc gtttatctcc aattgaagag gcgtataaga
    13861 agtcaaatct tcgtgtttta catccaaatc gaaattatac agtaacatct attttagtct
    13921 gtttcttctt tatgtgtttt gttggatgga tctgggaagt gagcctatct atgattagct
    13981 atggacgttt tgtgaatcgc ggagttttac atggtccatg gattcctatt tatggttttg
    14041 gctgtgtttt gattttgctt ttattaaaac gatttagaat gcgccccaaa gttgaattta
    14101 gtatggccgt acttctttgc ggatgtattg aatactttac aggtttcttt ttagagctta
    14161 cgcataatgg tcaaaaatgg tgggattata caggctactt tttgaatctt catggaagaa
    14221 tttgtgcgga aggtttattg gtgtttggcg ttggtggtat ggcttttgtc tatgtgattg
    14281 caccattaat tgataactgg gttaaagaac atttaaataa aagattatct actgcctgtt
    14341 tagttttact tcttttattt ggtgcagatg ttgtttattc acactttgaa cctaatgtag
    14401 gagaaggtgt tacaggatga taaaaaagtt aaatggaaat caaatcaaga tgattgcgat
    14461 tattgcgatg tttatggatc atttcgcaac gacgttgtat tcaaattatg aaaaattctg
    14521 gtggctttta ttgatacatt taattggacg agtggcagca cctattatgt ggtttatggt
    14581 tgtggaaggc tatacgcata cgcatagttt aaagaaatat ttagaaagat tattgatttt
    14641 ttcaatcatt gctcactttg cgtataactt ctgttttggc atatctttga ttccatttaa
    14701 agaatccttc ttaaatcaaa caagtattat atggccatta ttcttatctg tgattggttt
    14761 atgggtggaa gatcaagaat ggaaagatgc aaagaaaata ggttgtattc ttgttctttg
    14821 tttattggca tttccaagtg attggtcatg ttttccgatt ctttgcacaa atcatattta
    14881 taaaaatcaa ggcaatctaa aaaaacaagt actaggtatg gttctctata tttctatgta
    14941 tgtttttgtt tattgtctat gcatagatgt agtttatggt ttattacagt ttgggattgt
    15001 tttagtatat ccaattatgt ctttatatga tggaacaaga ggaaagagta cttttatgaa
    15061 atggttcttt tattggtttt atgtgggaca tttaattttg tgcggattga ttcgtgtaat
    15121 gatgtagagg tgaagaacgg agggtaaaaa aagaatgcat gaaataagtc gtaaaattct
    15181 taatcactat cagattcgaa aaacaaagaa acaaaaagaa gagtttcgta aatttttagt
    15241 agacgaatta aaacaatatg ggtattgtcc cattgttcaa aataaaggga taaacaataa
    15301 tattgttatc ggcaacatgg atacagctag gatattttgt acggctcatt atgatacaca
    15361 agcagtctta ccatttccaa atttaattgt gcctaataat ttgttcgggc tgattttgtc
    15421 acaatttgcg attattattt gtgtattgat tcttatattc tttatgaaca ttgtccttat
    15481 taaattattt gtttggacag gtctattcaa gaatattgaa cttaatattg cttctactat
    15541 ttcatggtta attattatga tatggatgtt gtttggaaaa gcaaataaac atactgcaaa
    15601 cgataatacg agtg
//
LOCUS       DBMDWL010000031        15258 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1319_length_15258_cov_2.861912, whole genome shotgun sequence.
ACCESSION   DBMDWL010000031 DBMDWL010000000
VERSION     DBMDWL010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 15258)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15258)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15258
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1319_length_15258_cov_2.861912"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="ACZPDN_03515"
     CDS             <1..1206
                     /locus_tag="ACZPDN_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR1297723.1"
                     /translation="KQVDEFFLFINNYFLNANTNAEKLGLDFKDEEFDELFKDNAFEW
                     IFIEGKMYLKDNFFENLIKVRKAYKDRLIEKDGAASTLLDDVMHKMKEEKDVYCKIHV
                     KTSLKVDPAFEKPGKTIRVWLPIPKEYAQVEDFKLLNTSHEGLVNDNSVDQRCVYIEK
                     PYEKGEEFSVEYEFINHMHYEELDPSIVTDKHPDTCLEEYAPHVVFTDYLKDLVKEII
                     GEETNPLLKAKLIYNYITTHVTYSYVRAYATLPCIPEYITTGLKGDCGLQALTFITLC
                     RIAGIPATWQAGLYTTPETIGNHDWARFYVAPYGWLYADCSFGGGSYRAKNFERQDFY
                     FGHLDPFRTPCSSKFQGDFVPAKNFLPNDPFDNQNGEIEYVDEAIPGKYIIKETEKIE
                     ISLLEDQNA"
     gene            1199..1909
                     /locus_tag="ACZPDN_03520"
     CDS             1199..1909
                     /locus_tag="ACZPDN_03520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR1297724.1"
                     /translation="MHRPIIGLTCNELDKENLPKQFINEAYINSVIRAGGCPIILPIT
                     NDYDTIQAQVNLLDGLIVTGGIDVNPMIYNENPEPLQGNSSLDRDYYEMRVLKYANEK
                     QIPIFGICRGIQMLNVYFKGSLYQDLSYCKRSVIKHAQQEKRENPSHKIHIEKDSFLY
                     PSLSDEAYVNSFHHQAIKDLAPHFKVAAKSSDGIIEAIEHESLPIYAVQFHPEALSHK
                     YGEMQDIFNVFIKKCEVK"
     gene            1909..3309
                     /locus_tag="ACZPDN_03525"
     CDS             1909..3309
                     /locus_tag="ACZPDN_03525"
                     /EC_number="3.4.16.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HIR1297725.1"
                     /translation="MRIQENLEKSKYEADGSLQIQGTTIPYHTICEDNFFVDEENNPV
                     ASIFSYAYFRSDIQDNSKRPILFIYNGGPGCASLWLHMGLFGPRIVKLDDELNLPTVP
                     PFELEDNPHCLLDLCDLVFIDPVGTGLGRLIQEKARKEFYETHGDVRSVSKFIEQFLA
                     RYNRRNSPVLLAGESYGTTRSALLAGELMGAGPEKADTMGISVSGIFLLGSYFIEKLP
                     VEASATDLITMAATNYFHNPKDDISQKDFIEEAFTFASTEYVTALFLGDACPNKEEIA
                     DKLAYYSGIDQTYWLRHHLHMDMQKGFAHKLLEDKGLALGFYDGRYTWNDDPEIMEAN
                     VIADDPAMGQYTPAFQTAYGLMRQELNITSDRISKGLVFDVNMTWNREFKTSPAQSLA
                     GCMRRNKQMKVFFASGLYDLCTTAGNARYLATHSNLDQNRVTIGEYPSGHMAYLGKES
                     FDLLAKDMRAFFESCI"
     gene            complement(3342..4787)
                     /locus_tag="ACZPDN_03530"
     CDS             complement(3342..4787)
                     /locus_tag="ACZPDN_03530"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1297726.1"
                     /translation="MKDKRNLSLVMDFYELTMSQCYFNDNKDKEVVFDLFYRKNPDDG
                     GYCVFAGLEQVVDYVSNLHFEKEDIDYLRSLNQFSEEFLEYLSTVRFTGDIYAIPEGT
                     PVFPHEPLVRVKAPIIEAQLVETALLLAINHQTLIATKSRRIVQASEGRAVMEFGARR
                     AHNFDAAILGARAAYIAGCAGSATTYSGKEFGVPVLGTMAHSFIQSFDTEYDAFMYYA
                     KTYPNACTLLIDTYSTLKSGIVNAIKVAKDYLEPNGYRLKGVRIDSGDMAYLSKKVRK
                     MLDEAGMEDCKIVVSNSLDEYVIESLIHQQGAKIDSFGVGENLICSKNSPVFGGVYKM
                     VAIEKDGQVIPKIKISDNVEKVTNPGMKNLNRIMDKETGMAIADLLTLEDEVIDTTQD
                     LTIYHPMSRWKYKVIPANTYTVRDMLVPIFKDGKLVYDLPSLEEIRAYSEKELATIWD
                     EIKRFVYPQEYYVDLSEKLLNLKLEMLKKYK"
     gene            complement(4798..5409)
                     /locus_tag="ACZPDN_03535"
     CDS             complement(4798..5409)
                     /locus_tag="ACZPDN_03535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR1297727.1"
                     /translation="MARLENKNYKSIQKSELNNPVVFVVDMIKGFVNIGPLHDEAIGN
                     VEKNIENLLINLECNNVFVCDSHPPKTREFNSFPAHCVIGSEESEVVDSLKPFVKHVI
                     KKNSTNTFMAPEFEEFLNSDLKYYRDIIVTGCCTDLCVLHFVLSLNTWFNEHNMNEYR
                     IIVVENCTETYHIPEVHEATFFNDVAFRMMEMNGIQVVSEVRE"
     gene            complement(5455..6471)
                     /gene="recA"
                     /locus_tag="ACZPDN_03540"
     CDS             complement(5455..6471)
                     /gene="recA"
                     /locus_tag="ACZPDN_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1297728.1"
                     /translation="MPKEKVETNKDKLLSDVLNEIEREYGKGSVMKLGDRAAVDIEPI
                     PSGSLLLDDALGIGGYPKGRIIEIFGPESSGKTTLALHAVAEVQKKGGRAAFIDAENA
                     IDPVYAKNLGVNIDELILSQPDSGEQGLDIMLMLVESGAVDLVVVDSVAALVPQAELD
                     GEMGDNQVGLQARMMSKAMRKLAGAMNQNDCTAIFINQLREKVGVIYGNPETTPGGRA
                     LKFYSSVRVDIRKADAIKNGTDIVGNKVKVKIVKNKVAPPFKSAVLEIMYGEGISYIS
                     ELLDLCVDANIVKKAGAWFSYEGEKIGQGKEAAKAYIKANPEFKSMLEEKLKEFRTNK
                     DVEE"
     gene            complement(6453..6947)
                     /locus_tag="ACZPDN_03545"
     CDS             complement(6453..6947)
                     /locus_tag="ACZPDN_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR1297729.1"
                     /translation="MNDARQLVSLCTENHLTISSCESLTAGLFTSTIASIPGASAILK
                     GGLVTYFTPMKSVLAHVDVDLIQKYGVISEECAYAMAKNTRQIMDVDYCISFTGNAGP
                     DAWEEKPAGRVYCAIASKKAIQVYGFQCTGLSRNDVREHVVMQMIDILIQTIQKENIC
                     QKKK"
     gene            complement(6947..7534)
                     /gene="pgsA"
                     /locus_tag="ACZPDN_03550"
     CDS             complement(6947..7534)
                     /gene="pgsA"
                     /locus_tag="ACZPDN_03550"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1297730.1"
                     /translation="MTKMNIPNRLTIIRILLVPVVVAVYLCMDPTLCVIDETSGLALR
                     DVIAFVIFAVASITDFFDGMLARKNNWITSFGKFADPIADKMLVNTVLILMVYFHQAN
                     VIAVLLMIARDLIVDGLRMSAANSGEVVSAGIFGKLKTVLQMFALVFLLLKDWPFVYM
                     GICMDQILLWAATVASLYSGLIYFNQLKKYVLETM"
     gene            complement(7494..8552)
                     /locus_tag="ACZPDN_03555"
     CDS             complement(7494..8552)
                     /locus_tag="ACZPDN_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024805.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297731.1"
                     /translation="MAYYHILKERRTNLKLSIQDVSNQTRLAPQYIQAIEEHNLDIFS
                     DDFSFVRYFVHAYCDAIGVNWQAIADEVDMDINEVAHQKDMALTMAQRRMVEQMASVQ
                     KKKKKSSTKKKKSWFQKHVSRTSSSLSTNQSQLIKVLVLIGIAGLCVLSVINFGLRAI
                     SNQKLQTQEALRQEQLSKKEQETNKLANQRQKAKEAAALVVEKISDETNAYQLSNIQS
                     DVKKLDLSITLPTKSKITVTKDGETINDDTKVYTGTFTQSLELSENCTFEISIETYSD
                     NSITIDGKEISFDKTDWQEGQPAVITLQVGKGNQKETEEYSQYDSDYDYGYSDEYYGQ
                     EGDYTYDQNEYTEQTNDY"
     gene            8640..9386
                     /locus_tag="ACZPDN_03560"
     CDS             8640..9386
                     /locus_tag="ACZPDN_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297732.1"
                     /translation="MSQLNFSIGTFSQCMSFSPQNIQIDNFEDCTLYCIDCENVQQFD
                     INTLNATSPSVFFFFGNFEKIGQKAWQQIFAIAKDFPNSYCFNTHQSQKNFADIYCAT
                     LLGQVVANVIPKKILLVTNDQGFHGIKQALMFQGKDIDIEILQTRDTFLLPLKEEQPK
                     QVQKAKKQPPQTNQKPNEASDEEVLEALKKYPKINTRYASIDDLSKYLMPILANKQKK
                     CKLSKWIESDYAKKVGRYKKDSKGKYYKLK"
     gene            9383..9877
                     /gene="msrA"
                     /locus_tag="ACZPDN_03565"
     CDS             9383..9877
                     /gene="msrA"
                     /locus_tag="ACZPDN_03565"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR1297733.1"
                     /translation="MIKTIYLAGGCFWGVQHYFDQFDGILKTIVGYANGKTQNPEYKD
                     VKARLTGHAETLEIQYDDKMISLSKILDLYFDIIDPVSVNQQGEDIGISYRTGIYYVE
                     KADLETIQSKYNEIQKKYDSPLAVEVEPMKNFFNAEEYHQKYLDKNPGAYCHIPASKF
                     KKGL"
     gene            complement(9874..11097)
                     /locus_tag="ACZPDN_03570"
     CDS             complement(9874..11097)
                     /locus_tag="ACZPDN_03570"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015451.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR1297734.1"
                     /translation="MLKVCVIGGGPSGLMAAYTASLEGHQVELFEKNKQLGKKLQLTG
                     HGRCNVTNNCSKDEFFKHVVHNEKFLYSSFSQFSNLDMIKFLKSNGLPTIEEDHGRMF
                     PGSNSSQDVVLLFVDLLRKNNVILHMDEACTNVVVEEDRVVGIKTDKGTYSFDVVIVA
                     TGGKSFVSTGSSGDGYQWAQNLDIKVSDLYPGLVALRTKEDFDLAGLSLSNVGIQIKK
                     GKKVLYKECGDILFTHEGLSGPAILAMSSYVINSKPERIILDLICDKSIDEVDMLLLE
                     RMNHSSNKKLSHILETMIPKRLVQYVMNRLHIDDSLKANETTKAQRRSISELLKCLSF
                     EIANFAGFDQAMITVGGIKTNQIQPQTMEAKKIKGLKFVGEVLDLDAQTGGYNLQIAW
                     TTGYVAGSTIKEGAN"
     gene            complement(11091..11900)
                     /locus_tag="ACZPDN_03575"
     CDS             complement(11091..11900)
                     /locus_tag="ACZPDN_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1297735.1"
                     /translation="MTTKLVICDIDATLVNEKKELTPRTKKDLIQLHEKGIYFGIASG
                     RPADELKRNKEYWDLPFDFDFVVGMNGAELWDGVHQKFYSYFMMKKEWLKETMEIMKV
                     VKCNPMIYWDGKIRCVHIDDWMIHSASTSHKELEVVKLEDFYAHENAKIMFRMKEEDM
                     PKAETWVKEHPNPYYHGFKTQSTLLEFCDKRINKGYGLKKICELNDLDIKDVMAFGDT
                     SNDNDMLKVAGCGVCMINGTDDTKACANAITKQDNDHDGLAIYLEESGILC"
     gene            complement(11897..12772)
                     /locus_tag="ACZPDN_03580"
     CDS             complement(11897..12772)
                     /locus_tag="ACZPDN_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1297736.1"
                     /translation="MKKTNYVKDVLVVLLGNFMVAVSVAFFVLPNNTLTGGVAGVAVA
                     LKPLLPFIPSVWLINILMIGLYIVGAVFLGKAFALKSLISTFSYSFFVSLLTYVTTLF
                     PKDTFVMQPMLASIYCGLISGIGLGLCFRVNASTGGMDIPALLIHKFTHISSGNSVMI
                     IDALTVLLGMSTYGVEPALIGVLSVFTSGFAIDKTILIGSSTAINCMVISDQWVEIKN
                     HVLDNMKRGVTVLEGKGGYTNESKPVLMIVIPQKMYSNLEHEIMKVDPRAFIIVNSVH
                     EVDGEGFTYELEDLK"
     gene            complement(12858..13280)
                     /locus_tag="ACZPDN_03585"
     CDS             complement(12858..13280)
                     /locus_tag="ACZPDN_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1297737.1"
                     /translation="MKKNEQVLEAWLQMTTVINNERITPDLPYNESMICRYLYQNQDL
                     NVTATDLCNYMRMQKSQMNRTLTSMEKKQLITRIRSEDDNRKIYVSLNDSMIKIYKKQ
                     HEKILKIVDELFEKIGYERQPEVIELFDLITQTAKEML"
     gene            complement(13285..14346)
                     /locus_tag="ACZPDN_03590"
     CDS             complement(13285..14346)
                     /locus_tag="ACZPDN_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR1297738.1"
                     /translation="MKVLKKIGITIICLLLICGIGIVCLFHKEYSSLKSLKKVDAYPM
                     YTMDYSADYGLDEFLEKGASNDKELVEFVVNHVMKGLPLSINIPDLGCSTFIAQNKDS
                     GYLFGRNFDMDYSPSVLVKTKPKNGYASVSMVNLGFVGYNEKHLPDTLKDSLVTLAAP
                     YAPLDGMNEKGLAVGVLLIDTKPTNQNTKKVDITTTTAIRLMLDKAKNVDEALELLSS
                     YDMHSSANSCYHFQICDASGKSVVVEYVDDEMKVVYPDKNYQCATNFLLTQPDAKFNF
                     GQDRYQIIDEKLSSTNGKLSKHEAMQLLSDCSQEAHKNKQGKISKTQWSCVYDLKNKK
                     VTICVNENYDAKYTISVLE"
     gene            complement(14431..15102)
                     /locus_tag="ACZPDN_03595"
     CDS             complement(14431..15102)
                     /locus_tag="ACZPDN_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR1297739.1"
                     /translation="MYTNNYSNSVSTNSLYKVYSWMMVGLGITAITSALLYASGIFML
                     IIQIPMISLLLFIVQISLTISMGRQLSRNTPASTMKTMFIVYSLTMGINMTSLAYVYS
                     AGQIAAAFGISAIYFACLAIIGKTTKMDLSKVGNICLIGLIAMIFSQFFFMLFRVGMD
                     VRLWSVIGLLLFTGLTAWDIQKMNYMLNGYEDEKISIYMALQLYLDFLNIFLYILRLL
                     GRRDD"
BASE COUNT         5049 a         2935 c         2210 g         5064 t
ORIGIN      
        1 aaacaagttg atgaattttt tttgtttatt aataactatt ttttaaatgc taatacaaat
       61 gcagaaaaac tcggtttaga ttttaaagat gaagaatttg atgaattatt caaagacaat
      121 gcgtttgaat ggattttcat tgagggaaaa atgtatttaa aagacaattt ctttgaaaac
      181 ctaattaaag tgcgtaaagc ttataaagat cgtttaattg aaaaagatgg tgctgcaagt
      241 actttattag atgatgtaat gcataagatg aaggaagaaa aggatgttta ctgcaagatt
      301 catgtcaaga caagcttgaa ggtagatcct gcatttgaaa agccaggaaa aacaattcgt
      361 gtatggcttc ctattccgaa agaatatgcg caggttgaag attttaaatt attaaataca
      421 tctcatgaag gcctagtaaa tgacaatagc gtagatcaaa gatgtgtata tattgaaaag
      481 ccatatgaaa aaggtgaaga attcagtgta gaatatgaat ttatcaatca tatgcactat
      541 gaagaattag acccaagtat tgtaacagat aaacatccag atacttgtct tgaagaatat
      601 gcgccacatg ttgtatttac agattactta aaggatctag ttaaagaaat cattggtgaa
      661 gaaacaaatc cattattaaa agcgaaattg atttataact atatcacaac acatgtgacc
      721 tattcatatg tacgtgctta tgcgacttta ccttgtattc cagaatatat cacaacagga
      781 ttaaagggag attgtggttt acaagcattg acatttatta ctctatgtcg tattgcgggc
      841 attcctgcta cttggcaagc tggactttat actacacctg aaaccattgg taatcatgac
      901 tgggcacgat tctatgtagc tccatatgga tggttatatg cggattgttc atttggtggt
      961 ggatcctatc gtgctaaaaa ctttgaaaga caagatttct attttggaca tttagatcca
     1021 tttagaacac catgctcatc taaattccaa ggtgattttg taccagctaa aaacttcttg
     1081 ccaaatgatc catttgataa tcaaaatggt gaaatagaat atgtggatga agccattcca
     1141 ggtaaatata tcataaaaga aaccgaaaaa attgaaatct ctcttttaga ggatcaaaat
     1201 gcataggcct attattggtc taacttgtaa tgaattggat aaagaaaatc ttccaaaaca
     1261 gtttatcaat gaagcctata ttaatagtgt cattcgtgcc ggtggctgtc ccatcattct
     1321 tccaatcaca aatgactatg atacaattca agctcaagtt aatctattag atggattgat
     1381 tgtgacaggt ggaatcgatg taaatcctat gatttataat gaaaatccag aacctttaca
     1441 aggtaattca agcttagatc gtgattatta tgaaatgcga gtattaaaat atgccaacga
     1501 aaaacagatt cctatttttg gaatctgtcg tggcatacag atgttgaatg tctattttaa
     1561 aggatctcta tatcaagatt tgtcgtattg taaaagaagt gtaatcaaac atgcgcaaca
     1621 agaaaaaaga gaaaatccaa gtcataaaat ccatattgaa aaggatagtt ttttatatcc
     1681 tagcttaagt gatgaagctt atgtgaatag ttttcatcat caagccatca aggatttagc
     1741 accacacttt aaagtagcag ctaaatcgag tgatggaatt attgaagcca ttgaacatga
     1801 gtctctacct atttatgccg tacagtttca tccagaagcc ttgtcccata aatatggtga
     1861 gatgcaagat atcttcaatg tctttattaa aaagtgtgag gtaaagtaat gcgtattcaa
     1921 gaaaacttag aaaaatcaaa atatgaggca gatggaagcc tacaaattca aggaacaacc
     1981 attccttatc atacaatttg tgaagacaat ttcttcgtgg atgaagaaaa caatccagtc
     2041 gcatccatat ttagttatgc ctattttaga agtgatattc aagacaattc aaaacgtccc
     2101 atcttattta tttataatgg cggtccagga tgtgcaagtc tttggcttca catgggattg
     2161 tttggtccta gaattgtaaa attagatgat gaactaaacc ttcctacagt tccacctttt
     2221 gaattagaag acaatccaca ttgtttatta gacttatgtg atttagtctt tattgatcca
     2281 gtaggaacag gtttaggtcg tttgattcag gaaaaggcaa gaaaagaatt ttatgaaaca
     2341 catggcgatg ttcgtagtgt ttctaagttc attgaacaat tcttagctcg ctataatcgt
     2401 agaaattccc cagtcttact agctggtgaa agctatggta ccacaagaag tgccttactt
     2461 gcaggagaac ttatgggtgc aggtcctgaa aaagccgata cgatgggcat tagtgtgagt
     2521 ggtatcttct tacttggaag ttactttatt gaaaagcttc ctgttgaagc aagtgctaca
     2581 gacttaatca caatggctgc cacaaattat ttccataatc ctaaagatga tatttcacaa
     2641 aaagacttta ttgaagaagc ctttacattc gcatccactg aatatgttac agccttattt
     2701 ttaggagatg cctgcccgaa taaagaagaa atcgcagata aattagccta ttattcgggt
     2761 attgatcaaa cctattggtt aagacaccat ttacatatgg atatgcagaa aggctttgcg
     2821 cataaactat tagaagataa aggtttagct ttgggattct atgacgggcg ttatacatgg
     2881 aatgatgatc cagaaatcat ggaagccaat gtcattgcgg atgacccagc tatgggacaa
     2941 tatacacctg ctttccagac tgcctatgga ttgatgcgtc aagaattaaa tatcacaagt
     3001 gatcgtatct caaaaggctt agtctttgat gtaaatatga catggaatcg tgaattcaaa
     3061 acaagtcctg cccaatcctt agctggatgc atgcgaagaa acaaacagat gaaagtcttc
     3121 tttgcctcag gtctatatga tttatgtact actgctggaa atgccagata tttggcaacc
     3181 cattccaatc tagatcaaaa tcgagttaca attggtgaat atccaagtgg acacatggct
     3241 tatcttggta aagaaagctt tgatctatta gccaaggata tgcgtgcatt ctttgaaagt
     3301 tgtatttaac acaaaaaccg tcaagcgacg gtttcttttt tttatttata tttcttcaac
     3361 atttctagct taagattcaa taacttttca cttaagtcaa cataatattc ttgtggatat
     3421 acaaaacgtt tgatttcatc ccaaattgta gctagttctt tttcactata tgcacgaatt
     3481 tcttctaggc ttggaagatc atatactagc ttaccatctt taaagattgg cactaacata
     3541 tcacgtactg tatatgtatt agctggaatc actttgtatt tccaacgtga cattggatga
     3601 tagattgtta aatcttgcgt tgtatcaatg acttcatctt ctaatgtcaa taaatcggca
     3661 atagccatac ctgtttcttt atccataata cgatttaaat tcttcatacc tggatttgtc
     3721 actttttcaa cattatcact gattttgatt tttgggatta cttgaccatc tttttcaatc
     3781 gccaccattt tataaacacc accaaatact ggtgaatttt ttgaacaaat caagttttca
     3841 ccaacaccaa acgaatcaat tttagcacct tgttgatgaa tcaaagattc aatgacatat
     3901 tcatctaatg aattggatac aacgatctta caatcttcca tacccgcttc atctaacatc
     3961 ttacgaactt ttttagaaag atatgccata tctccactat caatacgaac accttttaaa
     4021 cggtatccat ttggctctaa ataatcctta gctactttaa tggcattcac aatacctgat
     4081 tttaaagtag aatatgtatc aatcaataaa gtacatgcgt ttggatatgt tttggcataa
     4141 tacataaacg catcatattc ggtatcgaaa ctttgaataa atgagtgcgc cattgttcct
     4201 agtactggta caccaaattc tttaccagaa tatgttgtag cacttcctgc acatcctgca
     4261 atataagctg ctctagctcc tagaatagct gcatcaaagt tatgagcacg tcgtgcacca
     4321 aattccataa ccgctcttcc ttcactagct tgtacaatac gacgtgattt tgtcgcaatc
     4381 aaagtttggt gattaatagc caatagtaac gccgtttcta caagttgtgc ttcaataatt
     4441 ggtgctttta cacgaaccaa tggttcatga ggaaataccg gtgttccttc tggaatcgca
     4501 taaatatcac ctgtaaaacg tacggttgat aaatattcta aaaattcttc actaaattga
     4561 tttaatgagc gtaaataatc aatatcttct ttttcaaaat gcaaatttga aacataatcc
     4621 acaacctgtt ctaaacctgc aaatacacaa tatcccccgt catccggatt tttacgataa
     4681 aataagtcaa atacgacttc tttgtcttta ttatcattaa aataacattg actcattgtt
     4741 agttcataaa agtccatgac taaacttaaa tttctcttat ctttcatttt ttaaatctta
     4801 ctcccttact tcagaaacaa cttgaatacc attcatttcc atcattctaa atgcgacatc
     4861 attaaagaat gtagcttcgt gtacttctgg tatatgatag gtttctgtac agttttcaac
     4921 tacgataata cgatattcat tcatattgtg ttcattgaac caagtattta aacttaacac
     4981 aaaatgaagc acacaaagat ctgtacaaca acctgttaca attatatcac gataatattt
     5041 taaatctgaa tttaaaaact cttcaaattc aggtgccata aacgtattcg tactgttctt
     5101 cttgatgaca tgtttcacaa acggctttaa agaatcaact acttcagatt cttcacttcc
     5161 aattacacaa tgtgctggaa acgaattgaa ttcacgcgtc tttggaggat gtgaatcaca
     5221 cacaaaaaca ttgttgcatt ctaaatttat caaaagattt tcaatattct tttccacatt
     5281 gccaatggct tcatcatgaa gtgggcctat attcacaaac ccctttatca tgtccaccac
     5341 aaatacaacc ggattattta attcactttt ttgaatggat ttataattct tattttctaa
     5401 acgtgccata taatatatac ctctaaatga aaataagagt tctacgcaga actcttattc
     5461 ttcaacatct ttgtttgttc taaactcctt taatttctct tcaagcattg atttgaattc
     5521 tggatttgcc ttgatgtaag ctttggcagc ctcttttccc tgtccaattt tttctccttc
     5581 atagctgaac catgcccctg ccttttttac gatattggca tcaacacata aatctaatag
     5641 ttcactaata taagagattc cttctccgta cataatttca agtacagccg atttaaatgg
     5701 aggagctacc ttatttttaa caatttttac tttgacttta ttccctacaa tgtctgtacc
     5761 atttttaatg gcatctgcct tacgaatatc cacacgaaca gatgagtaga atttcaaagc
     5821 tcgcccaccc ggtgttgttt ctggatttcc ataaataacg ccgacttttt cacgaagctg
     5881 gttgataaaa atagctgtac aatcattttg gttcattgct ccggcaagct tacgcatagc
     5941 cttagacatc atacgagctt gtaatccaac ttgattatca cccatttccc catctaattc
     6001 ggcttgtgga actaaagccg ccacactatc tacaacaacc aaatcaacag ctccactttc
     6061 tacaagcatc aacataatat ctaaaccttg ttcacctgaa tctggttgag acaaaattaa
     6121 ttcatcaata ttaacaccta gattttttgc gtatacagga tcgatcgcat tttccgcatc
     6181 aataaaggct gctcgaccac cctttttttg aacttcagca acggcatgta aagcaagcgt
     6241 tgttttacca cttgattccg gaccgaagat ttcaataatt cttccctttg gatatccgcc
     6301 aatacccaag gcatcatcta aaagtaaact tcctgaagga ataggttcaa tatcaacagc
     6361 cgctcgatcc ccaagtttca tgacagaacc tttaccatat tccctttcga tttcatttaa
     6421 aacatcactt aaaagcttat ctttatttgt ttctactttt tcttttggca tatattctcc
     6481 ttttgaattg tttgaatcaa tatatcaatc atctgcataa caacatgttc tctaacatca
     6541 tttctagaca aacctgtaca ttgaaaacca tacacttgaa tagctttttt tgatgcgatt
     6601 gcacaatata ctcttcccgc tggtttttct tcccaagcat ccggtccagc atttcctgta
     6661 aaagaaatac aataatccac atccataatt tgtcttgtat ttttagccat cgcatacgca
     6721 cactcttcac tgattacacc atatttttga attaagtcca catccacatg agccaataca
     6781 cttttcattg gtgtaaaata agtcactaag ccacctttta atatggcact tgccccaggt
     6841 atggaggcaa tcgtactcgt aaataatcca gccgttaaac tttcacagct agaaatggtt
     6901 aaatgatttt ctgtacataa agaaactagt tgtcttgcat cattcattac atagtctcca
     6961 aaacatattt ttttaattga ttgaaataaa tcaatccact atataaagaa gccactgttg
     7021 ctgcccacaa caaaatttga tccatacaaa tacccatata gacaaatggc caatctttca
     7081 acaataagaa cacaagcgca aacatttgta gtacagtttt taattttcca aaaattcccg
     7141 cactcacaac ttcaccagaa tttgcagcac tcattcttaa cccatctaca atcaagtctc
     7201 ttgcgatcat caataatacg gcaatcacat tggcctgatg aaaataaacc atcaagatca
     7261 atactgtatt gactaacatc ttatctgcga ttggatcggc aaattttcca aatgaagtaa
     7321 tccaattgtt ctttcttgcc aacattccat caaagaaatc ggtaatactc gcaaccgcaa
     7381 agatcacaaa cgcaattaca tctcgaaggg ctaagccact tgtttcatca attacgcaaa
     7441 gagtaggatc catacataaa tatacagcta cgactaccgg tactaataaa attctaataa
     7501 tcgttagtct gttcggtata ttcattttgg tcatatgtat aatccccctc ttgtccataa
     7561 tattcatctg agtaaccata atcatagtca ctatcatatt gtgaatattc ttcagtctct
     7621 ttttgatttc cttttccaac ctgtaaagtt ataacagctg gttggccttc ttgccaatct
     7681 gttttatcaa aagaaatctc ttttccatca atagtaatcg agttatccga atatgtttca
     7741 atactaattt caaaagtaca attctcggat aattctagag actgcgtaaa tgtacccgta
     7801 tatacttttg tatcatcatt gatcgtttca ccatcttttg tcacagtgat ctttgactta
     7861 gtcggtaaag taatacttaa atctaatttt tttacatccg attgtatatt tgaaagctga
     7921 tatgcattgg tttcatcact tattttttcg acaactaaag cggctgcttc ctttgccttt
     7981 tgacgttgat ttgccaattt attggtttcc tgttcctttt ttgaaagctg ttcttgacgc
     8041 aaagcctctt gcgtttgaag tttctgattt gaaatggcac gaagcccaaa attaatgaca
     8101 gataaaacgc ataaacctgc aattccaatc aggacaagca ctttaatcaa ttgcgattga
     8161 tttgtactca aagaagaact tgttcgactt acatgttttt gaaaccaaga ttttttcttt
     8221 tttgtcgatg acttcttttt ctttttttga acacttgcca tctgctctac cattcgtctt
     8281 tgtgccattg tcaaagccat atctttttga tgagccactt cattaatatc catgtccact
     8341 tcatcagcga ttgcttgcca atttacacca atggcatcac aatatgcatg tacaaaataa
     8401 cgaacaaaag aaaaatcatc cgaaaaaata tctaagttat gttcttcgat agcttgtata
     8461 tattgaggcg ctaaacgcgt ctgatttgaa acatcctgaa tggaaagttt tagatttgta
     8521 cgacgttctt ttaagatgtg ataataagcc actaaaactc cccctttgaa gtttattata
     8581 acaaataaaa ggaaagaata ccatgttgtg ctataattat ttatgaaagg atgaatgaaa
     8641 tgtcacaact taatttttca attggaacat tttctcaatg catgagtttt tcacctcaaa
     8701 atatacaaat cgataatttt gaagactgta cactctattg tattgattgc gaaaatgtcc
     8761 aacaattcga tattaataca ctgaacgcca caagtcctag cgttttcttt ttctttggaa
     8821 actttgaaaa aataggacaa aaagcatggc aacaaatctt tgcgattgcc aaagattttc
     8881 ctaattccta ttgtttcaat acacaccaaa gtcaaaagaa ttttgcggac atctattgtg
     8941 ctacacttct tggtcaagtt gtcgccaatg ttatacctaa aaaaattctt cttgtcacaa
     9001 atgatcaagg atttcatggc attaaacaag cattaatgtt tcaaggtaaa gacatcgaca
     9061 tcgaaatttt acaaacaaga gatacattct tattgccatt aaaagaagaa cagccaaaac
     9121 aagttcaaaa agcaaaaaaa caaccgccac aaacaaatca aaagccaaat gaggcaagtg
     9181 atgaagaggt tttagaagct ttaaaaaaat atcctaaaat caatacacgc tatgcatcga
     9241 ttgatgattt aagcaaatat ctaatgccta tcttagcgaa taaacaaaag aagtgtaaac
     9301 tttcaaaatg gatcgaaagt gattacgcaa aaaaagtagg tcgatataaa aaggattcga
     9361 aaggaaaata ttataaatta aaatgataaa aacaatatat ttagcaggtg gctgcttctg
     9421 gggggtacaa cactattttg atcaatttga tggcatctta aaaacaattg ttggatatgc
     9481 gaatggtaaa acgcaaaatc ccgaatataa agatgttaaa gcaaggctta caggtcatgc
     9541 agaaacatta gaaattcaat atgatgacaa aatgatttca ttatctaaaa tattagactt
     9601 atactttgat atcattgatc cggtatccgt caatcaacaa ggtgaagata tcggtatttc
     9661 ctatcgtaca ggtatttatt atgtggaaaa agcagattta gaaaccattc aatctaaata
     9721 caacgaaatc caaaagaaat atgattcacc tctagctgtt gaagtagagc ctatgaaaaa
     9781 cttttttaat gccgaagaat atcatcaaaa atatctagac aaaaatccag gtgcttattg
     9841 tcatattcct gcatcaaaat ttaagaaggg actttaattg gccccttctt ttattgtgct
     9901 tcccgcaaca tatcctgttg tccatgcgat ttgtagattg tatccacctg tttgtgcatc
     9961 caaatctaaa acttctccaa caaattttaa acctttaatt ttcttagctt ccattgtctg
    10021 aggttgaatt tgattggtct tgattccacc cacagtaatc atagcttgat caaaacctgc
    10081 aaaatttgcg atttcaaaac ttaagcactt taataattca ctaatacttc ttctttgtgc
    10141 ttttgtggtt tcattggctt ttaaagaatc atcaatatgt aatcgattca taacatattg
    10201 aaccaatcgc ttaggaatca ttgtttctaa aatatggctc aattttttat tcgaagaatg
    10261 attcattcgt tctaacaaaa gcatgtctac ttcatcaatt gatttatcac aaattaaatc
    10321 taaaataatt cgttcgggtt ttgaattaat gacatagctt gacatggcca aaattgcagg
    10381 tccacttaaa ccttcatggg taaataaaat atcaccacat tctttataaa gaactttttt
    10441 acctttcttt atttgaattc caacattaga caatgaaagt ccagccaaat caaagtcttc
    10501 cttagtgcgt aaagcaacta atccaggata caaatcactt acttttatat ccaaattttg
    10561 agcccattga tatccatctc cactacttcc tgtggataca aatgattttc ctcccgttgc
    10621 gacaatgact acatcaaatg aatatgttcc tttatccgtt ttgattccaa caactcgatc
    10681 ctcttcaaca actacatttg tacaagcctc atccatatgt aggatgacat tatttttgcg
    10741 taataggtct acaaacagta aaacaacgtc ttgacttgaa tttgaaccag gaaacatacg
    10801 tccatgatct tcttcaatag taggtaaacc attggatttt aaaaatttga tcatatccaa
    10861 attcgaaaat tgtgaaaaag aactatacaa gaatttttca ttgtgtacaa catgcttaaa
    10921 aaattcatct tttgaacaat tgtttgtcac attacaacgc ccatgacctg tgagttgtaa
    10981 tttcttaccc aattgtttat tcttctcaaa caattctact tgatgtcctt ctaaacttgc
    11041 cgtataggca gccatcaatc cggatggacc ccctccaatt acgcatactt ttagcataga
    11101 atcccacttt cttctagata aatagctaag ccatcatgat cattatcctg ttttgtgatg
    11161 gcattggcac aagcttttgt atcatccgta ccattgatca tacaaacacc gcaacccgct
    11221 acttttaaca tgtcattatc attggatgta tctccaaagg ccatgacatc ctttatatct
    11281 aaatcattga gttcacatat ttttttaagt ccatacccct tattgattct tttatcacaa
    11341 aattcaagta gtgtggattg tgttttaaaa ccatgataat aaggatttgg atgttctttt
    11401 acccaagttt ctgccttagg catatcctct tctttcatac gaaacatgat ttttgcattt
    11461 tcatgtgcat agaaatcttc taatttcaca acttccaatt ctttatgcga tgtcgaagcc
    11521 gaatgaatca tccaatcatc aatatgaaca caacgaatct ttccatccca atatatcatt
    11581 ggattacatt tgacaacttt catgatttcc atggtttctt ttagccattc ctttttcatc
    11641 atgaaatagc tgtagaattt ttgatgcact ccatcccaaa gttctgcgcc attcatccct
    11701 accacaaaat caaagtcaaa aggtaaatcc caatactctt tattgcgttt taattcatct
    11761 gccggacgac cagatgcaat gccaaaataa atcccttttt catgaagttg aattaaatct
    11821 ttcttcgttc taggtgtcaa ttctttcttt tcattcacta atgtcgcatc aatgtcacaa
    11881 atcactaatt ttgtcgtcac tttaaatcct ccaattcata agtaaatcct tcgccatcga
    11941 cttcatgaac actattcacg ataataaacg ctcttggatc caccttcatg atttcatgtt
    12001 ctaaattcga atacattttt tgtggaataa caatcatcaa tacaggtttg gattcgtttg
    12061 tatatccacc cttaccttct aaaactgtaa ctccacgttt catgttatct aacacatgat
    12121 ttttaatttc aacccattgg tccgaaatta ccatacagtt aatagctgta cttgaaccaa
    12181 tcaaaatcgt tttatcaatc gcaaaaccag atgtgaatac agataaaact ccaatcaaag
    12241 ctggttctac accatatgta gacattccca ataatacagt taaagcatca atgatcatta
    12301 cggaatttcc agaagaaata tgcgtaaatt tatgaatcaa taatgcagga atatccattc
    12361 ctcctgtact tgcgtttact ctaaaacaga gtcctaagcc aatacctgaa atcaacccac
    12421 aataaatact tgccaacatg ggttgcatca caaatgtatc ttttggaaat aaagttgtca
    12481 cataagtcaa caaagaaacg aaaaacgaat aactaaatgt tgaaattaat gatttcaatg
    12541 cgaatgcctt tcctaaaaaa acagctccca caatataaag tccaatcatc aatatgttga
    12601 tcaaccatac cgatggaata aatggcaaca acggttttaa tgccactgcc acaccggcta
    12661 cgcctccagt taatgtatta tttggtaaaa caaaaaaagc aacggatact gccaccataa
    12721 aatttcccaa taatacaacc aatacatctt ttacataatt tgtttttttc atataaacct
    12781 ccccaaacgc catctttatt ctatcaaaaa agaaggcttt atgaaacaaa agaaaaagct
    12841 gggtatccag cttatcttta cagcatttct tttgcggttt gtgtaatcaa atcaaacagt
    12901 tcaatcactt caggttgtct ttcataacca atcttttcaa acaattcatc tacaattttt
    12961 aaaatctttt catgttgttt cttatagatt ttaatcatcg aatcatttaa tgaaacatag
    13021 atttttcgat tgtcatcctc actacgaata cgtgtaatca attgtttttt ttccatactt
    13081 gtaagcgtac gattcatctg cgatttttgc atgcgcatat agttgcataa atccgttgcg
    13141 gttacattta aatcttgatt ttgatataaa taacgacaaa tcatcgactc attgtaagga
    13201 agatctggag tgattctttc attattgata acagttgtca tttggagcca tgcttctaat
    13261 acttgttcat tctttttcat aattttattc tagcacagaa attgtgtatt tcgcatcata
    13321 attttcattc acacaaatcg ttaccttttt attctttaaa tcatatacac aagaccattg
    13381 tgtctttgat atttttccct gtttgttttt atgcgcttct tgcgaacaat cggataaaag
    13441 ttgcatagcc tcatgtttgg atagttttcc atttgtggaa cttaattttt catcaataat
    13501 ttggtagcgg tcttggccaa aattaaattt tgcatcgggt tgagtcaata agaaatttgt
    13561 agcacattga taattcttgt ctggatatac aaccttcatt tcatcatcca catattctac
    13621 aacaacactc tttccagaag catcacaaat ttggaagtga taacaactat tcgcacttga
    13681 atgcatgtca taagatgata ataattctaa tgcttcatcc acatttttgg ccttatcaag
    13741 cattaaacga atcgctgttg tcgttgtgat atccaccttt tttgtattct gatttgttgg
    13801 ttttgtatca atcaacaata caccaaccgc taatcctttt tcattcattc catctagtgg
    13861 tgcataaggg gctgccaaag ttactaatga atcctttaat gtatctggta aatgtttttc
    13921 attataccca acaaaaccta aattcaccat ggatacagag gcatacccat ttttaggttt
    13981 cgtcttcact aatacacttg gcgaataatc catatcaaag ttgcgcccaa acagatatcc
    14041 tgaatcttta ttttgtgcaa taaatgtaga acaacctaaa tctggaatat ttatgcttaa
    14101 tggtaatccc ttcattacgt gattcaccac aaattcgaca agctctttat cattgcttgc
    14161 acctttttct aaaaactcat ccaatccata atccgcacta taatccattg tatacattgg
    14221 ataagcatct acttttttta atgattttaa tgagctatat tccttgtgaa acagacaaac
    14281 aattcctatt ccacaaatca ataataaaca tattatagta attcctattt tctttaatac
    14341 tttcatacat actcctttag ttcattttgt gaactattaa agtatacaat attagtttac
    14401 attgtcaacc ataaaaaaat agagatgaaa ctaatcatct ctacgtccta ataatcgcaa
    14461 aatgtataag aagatattca agaaatctaa atatagctgt aacgccatat aaatcgatat
    14521 tttttcatct tcatagccat tcaacatata attcattttt tgtatgtccc aagctgtaag
    14581 tccagtaaat aataagagtc caatcacgct ccataagcgt acatccatgc caactctaaa
    14641 tagcatgaag aaaaattgac taaagatcat cgcaatcaaa ccaatcaaac aaatgtttcc
    14701 cactttacta agatccattt tagtcgtctt tccaataata gctaaacatg caaagtaaat
    14761 tgcgctaatg ccaaaggcag ctgcaatctg tccagcacta tatacataag ccaatgatgt
    14821 catattgatt cccattgtaa gtgaatacac aataaacatc gtcttcattg tggaagcggg
    14881 tgtattacgt gataattgac gtcccattga aatcgttaac gaaatttgta ctatgaataa
    14941 taatagtgaa atcatgggaa tctgaataat aagcatgaat atgcctgaag catacaacaa
    15001 ggccgaagtg attgccgtaa ttccaaggcc taccatcatc caactgtata ctttatataa
    15061 tgaatttgtg ctaactgagt ttgaataatt gtttgtatac ataaataaat ttacctccta
    15121 tttttagagt attttatgtg ttttacatat acattacaat gtacaactta gacaaaatga
    15181 ctattcaatt aacaaagtct tcaaaattta acctttaaaa gtagcttaaa ttactaagtt
    15241 tttcagtttt aaccttta
//
LOCUS       DBMDWL010000032        15123 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1329_length_15123_cov_2.680069, whole genome shotgun sequence.
ACCESSION   DBMDWL010000032 DBMDWL010000000
VERSION     DBMDWL010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 15123)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15123)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15123
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_15123_cov_2.680069"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1056..1132)
                     /locus_tag="ACZPDN_03600"
     tRNA            complement(1056..1132)
                     /locus_tag="ACZPDN_03600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1096..1098),aa:Arg,seq:tct)
     gene            complement(1186..3009)
                     /locus_tag="ACZPDN_03605"
     CDS             complement(1186..3009)
                     /locus_tag="ACZPDN_03605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297740.1"
                     /translation="MNNRSTNSLKRVIKETITSYKIESFIVLVLIIVSSIANVYGSMF
                     LKSLIDDYIVPLLSQTVPNYVPLLNALMKLVMIYGVGILATYGFNRIMVTISLGTLKH
                     IRDELFEHMQTLPLRFFDTHAHGDIMSVYTNDTDTLRQLISQSIPQVIVSLMTIISVT
                     ISMFILNVPLAVLTIACGLLMVYTSKKISAKSGKYFIAQQKQLGIENGFIEEMMEGSK
                     VIKVFTHEEQSIKDFDQVNDALFEASANANTFANVLMPVVMNIGYISYVLIAVMGSIF
                     ALNHIAGLTLGGIAAFLQLNRSFTNPIGQISMQLNAVIMADAGAKRIYEIIDTESEVD
                     DGVVTLEQIDHDHWAWHHPRKNGQDELVPLRGDVRFENVNFSYNPDKQILFDVSLFAK
                     PGQKIAFVGATGAGKTTITNLINRFYDIQSGSITYDGIDVKLIKKADLRKSLGIVLQD
                     TNLFTGTIMDNIRYGCLDASDEECIAASKLSNAHEFIKHLEHGYDTMIHAGGESLSQG
                     QRQLLSIARAAVANPPVLILDEATSSIDTHTERIVQEGMDKLMEGRTVFVIAHRLSTI
                     QNSDAIMVLEQGRIIERGNHEDLLKQKGKYYQLYTGAFELD"
     gene            complement(2999..4729)
                     /locus_tag="ACZPDN_03610"
     CDS             complement(2999..4729)
                     /locus_tag="ACZPDN_03610"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1297741.1"
                     /translation="MIKTLLKEVKEYKKDTLLSPVYVSIEAILEVLIPFLMAILVDNG
                     IEKGDMKSIWFYGFLMLVAAFVSLWAGAKSGKHAAIASSGFAKNLRKAEYRKIQEFSF
                     SNVDAYSTSGLITRMMTDVTNIQNAYQMMIRVCVRAPLMLIASLVMAFIINKELAMIF
                     VAAILVLGVVLFGIIFIVSPIFSRLFSRYDFLNASIQENILGIRVVKSFVRKDHEIEK
                     FRKESETIFSIQGKAEKILVFNSPVMQFCMYGTILLISWLGAHQIVASNMTTGELMSL
                     FTYTASILMSLMMMSMVFVMVTMSIASGKRIAEVLNQESDLTSPENGIQTVENGTIDF
                     EDVSFHYGNDEDILSHINLHIPSGSTLGILGATGSSKSSLVQLIPRLYDVTQGSVKVA
                     GMDVRDYDLKVLRDNVAMVLQKNVLFSGTIKENMRWGNPNATDDEIMEACKLAQADEF
                     IQLMPNKYDTYIEQGGSNVSGGQRQRLCIARAILKSPKILILDDSTSAVDTKTDALIR
                     QAFLEKIPHTTRIIISQRVSSIQDADQILVIDDGKISGLGTHEELLKSNVMYKETFEA
                     QNNGGDFDEQ"
     gene            complement(4740..5165)
                     /locus_tag="ACZPDN_03615"
     CDS             complement(4740..5165)
                     /locus_tag="ACZPDN_03615"
                     /inference="COORDINATES: protein motif:HMM:NF047172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1297742.1"
                     /translation="MRDNDEIGFYFSLIKKKMDKHMNEGLKKYDLTKSQRDVLGYLHF
                     TDKDPVIQKDIEEHFHISNPTVTGILNRLEQKGFIERKHNPKDKRVRTIVLTQKEQDL
                     HEDIEKQIFIMESKFENALGIEKRKQLLEILKELAQNLE"
     gene            5276..>5967
                     /locus_tag="ACZPDN_03620"
     CDS             5276..>5967
                     /locus_tag="ACZPDN_03620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014047.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR1297743.1"
                     /translation="MKKNEEYIVTCIDDTDLNSGVVKIDDMVVFVPNLLIGEKAKIKI
                     VKVNKKYAFGIIIELLKPSINRQELTCEVAKTCGGCQLQHMSYAYQLDYKYKHVKKLF
                     EDIEVKPILGMDNPWYYRNKAQFPVKIKDGKVQMGFYRQHSNDVVTCKECKIQDHVIN
                     KVFSFIQKKITLKMAQDLRHVFIRHSSTDEIQVVFIGKNEKNIISLSDQLKNTFANIT
                     SIVFNYNTRNDNV"
     assembly_gap    5968..5977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5978..6566
                     /gene="rlmD"
                     /locus_tag="ACZPDN_03625"
     CDS             <5978..6566
                     /gene="rlmD"
                     /locus_tag="ACZPDN_03625"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1297744.1"
                     /translation="YKVLYGNDSIIEECLGNKVKLHFKAFFQVNPKQMEVLYSQAIKA
                     ANLSGNETCIDMYAGTGTIGMAVSQHAKSVIGVEIVKEAVDNANDNTKMNHLDHCHYV
                     CQDATDFAHDHKEDKIDVIFIDPPRKGMTENGIKDTITMQPDKIVYVSCNPKTLSRDL
                     KIFKEYGYQCEYVQPVDMFCQTIGLECVAKLTKIV"
     gene            6581..6666
                     /locus_tag="ACZPDN_03630"
     tRNA            6581..6666
                     /locus_tag="ACZPDN_03630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6615..6617,aa:Leu,seq:gag)
     gene            complement(6731..7024)
                     /locus_tag="ACZPDN_03635"
     CDS             complement(6731..7024)
                     /locus_tag="ACZPDN_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR1297745.1"
                     /translation="MKGRRKKIQHVFVYFVMLGMSAFLIMGGMRQLAMTHELRTSINQ
                     KQKEKKALSSKKKELEKAKQNLTNPDYVEYIARGKYLVTKEGEQVFKFPSKDN"
     gene            complement(7078..8925)
                     /gene="mnmG"
                     /locus_tag="ACZPDN_03640"
     CDS             complement(7078..8925)
                     /gene="mnmG"
                     /locus_tag="ACZPDN_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1297746.1"
                     /translation="MYDVIVIGAGHAGIEACLASSRMNKKTAIVTLSKDMIGSMPCNP
                     SVGGPAKGIVVREIDALGGMMPIAADKTALQFKMLNTTKGPGVWSLRVQSDKIAYKHF
                     MKEALEKQDHLDIIEAACKSVVIKDGKAIGVELEDGTFIESKAVVLTAGTYMTSNVLR
                     GHTSTVSGPEEQRTVNTLSKSLRDAGIRTFRLKTGTPARVKMDTIDFSKGEPQPGTNQ
                     FLRFSETTNPEDVLPFEKQEICHLIYTQPKTHEIIHTHLHDSAMYSGLVKGVGPRYCP
                     SIEDKLVRFADKERHQLFLEPESKELDTIYIQGFSTSMPIDVQEEMVHSLPGLEHCVI
                     EKYAYAIEYDAIDPLQMKPNMESKIVENLFTAGQVNGTSGYEEAAGQGLMAGANAALK
                     VDGKEPFVLRRDEAYIGVMLDDLCTKGTKEPYRLLTSRAEYRLLLRHDNADQRLLEKG
                     YEIGLVSQERYDAFKKKMEAIEVAREELSNAHIKPNSDVDEYLKSLGFEPLAHGCSAL
                     DLIKRPKITVKGLAKYTGLDYEDQINEQIEIQTKYAGYIAKAKRDAKHLQQMEKMKLA
                     HDLDYENMDNLSLEARQKLTEIRPLTLGQASRISGINPSDIAILAMRVK"
     gene            complement(8927..10534)
                     /locus_tag="ACZPDN_03645"
     CDS             complement(8927..10534)
                     /locus_tag="ACZPDN_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1297747.1"
                     /translation="MSKSSAAKKSIIVGGLIGTGGLFVSKLIGLAYTIPFSYILQSEA
                     YQSVYAQSYNIYAYLLTIFQSGVPFAVATLVARYIALEDAKSVILVKKIAFAILGLTG
                     FVGMILLFTLSNVIAPAMVAKNADIMANCLKILSLAIFLVPVLSAFRGYYQGLKEMEE
                     YAFSQAFEQVFRVAFLLSAACLVVYAFGWDKKYALYAAVMSTSVATIAAIAQFAYFDR
                     KHQYVVDKMASKQTKACHSAKKIFREILILAVPYLVVAILGNIDQVFNGFLLPTGLKM
                     HYNAKDTTTVISASNYVAGKLNAIPMILGPGFATAIIPHISEALSKKNYKLVKKNVLD
                     SINVVLYVAIPVSFCIFAYAGPLNYTLYYSENLELCTFVVQWMALEGFLSTFAPLVTN
                     LMVSLELRKNVLKNLAIGVCIKGVLLIPFVWIMGIAGAVISSLLGTGYILYKNLKEIH
                     DVYHISYRKTMIISVRILVGLLALWITSILLSKLGLYGVDGSKLVCLFKMCLNGLLSV
                     IVYVVVTLYLKVPQSIFHFQVRKKSGV"
     gene            10636..12132
                     /locus_tag="ACZPDN_03650"
     CDS             10636..12132
                     /locus_tag="ACZPDN_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR1297748.1"
                     /translation="MYPAYIHTQATARNIDHAAIETYQIPSLILMEHAAIESTKIIEK
                     HITIDKSICILCGPGNNGADGLAIARLLQTKYSKLFVVVPEVKKMSEEEKIQFQMIQN
                     LNIEYDYNLPNEAYEVYIDCIFGNGLSRDITGFYKAMIQNVNATHSTIISIDMPSGID
                     ATMGNILGCAIHADYTISLDCYKQGQWLNDGKSHCGKLYLVDIGIPQILHQKCMDIIK
                     VLNKEDIHIPNRPLTAHKSTFGKALMIGGSQNMHGAIHMASLSAYKSGIGTLTLMVPE
                     CISNILAIKSDFYMLLQCSDRNGTFSSKAIEVLKQNMNNYSILSIGNGMQKNNVTISL
                     VEQVLKSDKKVVLDADALWAAQKNMDLLKRNETTILTPHIKEMSDLTGINITTILSNP
                     FEIARDFSKEYPSCVLILKSSQTYIAHQGNVYVLDAQNAGLAKGGSGDILCGIVVAML
                     GQCKDPMQAALCATYLHSQSAKLDIDLASFMPEDIINNIPNTLKRLRS"
     gene            complement(12129..12914)
                     /locus_tag="ACZPDN_03655"
     CDS             complement(12129..12914)
                     /locus_tag="ACZPDN_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HIR1297749.1"
                     /translation="MIDYTEGSNKQYHTQLSVNDVGDYVILTGDPKRVKDIASYLEDS
                     YFVADNREYVTYSGYMNGILCSVVSTGIGGASTAIAIEELVRLGCHTFLRVGTCGGIR
                     LDVQGGDIVIASGAIRQEGTTREYAPIEFPAVPSFDVLSAQVESAKKLCLPSHVGVVQ
                     SKDSFFGQHAPETMPVYPELQNKWNAYCKLDCLASEMESSTLFIVSQTRHVRSGAMFL
                     CLANQEREKAGLSNIQNHDLKPLMECAVQTIKILIEKDEAENH"
     gene            complement(12923..13222)
                     /locus_tag="ACZPDN_03660"
     CDS             complement(12923..13222)
                     /locus_tag="ACZPDN_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297750.1"
                     /translation="MKIRLHVVVDKEDDAIVELVQNALNEICSKMSYSPSRLQPSLAG
                     CMEFYATSELEENEIDDLLSKLNNDWDGEKDDCQAYSFNTTMFHPNVYYLQFQSF"
     gene            complement(13222..13509)
                     /locus_tag="ACZPDN_03665"
     CDS             complement(13222..13509)
                     /locus_tag="ACZPDN_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297751.1"
                     /translation="MTKSIPSSGAGAVRIILKNKDAFHFDLREKKEDNGKQSYLFDVY
                     YENATGTLNVLMDNGEPVIAALNLSLGKVITLSNDTNLKKLCKYVIDQVNA"
     gene            complement(13519..14982)
                     /locus_tag="ACZPDN_03670"
     CDS             complement(13519..14982)
                     /locus_tag="ACZPDN_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1297752.1"
                     /translation="MNKIGFNNQKYLEMQSKHIMERIAQFGDKLYLEFGGKLFDDYHA
                     SRVLPGFSPDSKLQMLMQLKDDAEIVIVISANDIEQNKTRSDLQITYQEDVLRLINEF
                     TKRGLYVGSVVVTQYNRQKAVDLFKARLKRRKIDVYFHYYIEGYPTDTKKIISDSGFG
                     KNDYVKTTRPLVVVTAPGPGSGKMATCLSQLYHEHKHGIKAGYAKYETFPIWNLPLNH
                     PVNLAYEAATADLADVNMIDPFHLQAYNEVTVNYNRDIEIFPVLKNIFEEIYGSSPYQ
                     SPTDMGVNMAGLCISDDDACCNASNQEIIRRYFVSKTRYAHELCSHEEVQKQEVVMNK
                     AGITELDRPCVMAARKKEEDSHSFSGAIELDDGTIITGKTTNLMGACSSVLMNVLKYL
                     AGIDKNKVLISPEAIVPIQNLKTAYLGSVNPRLHSNEILIALSVSALHSEDAKKALAM
                     LPKLKGMQAHVTCDVADVDLSIYSNLGIMLTYDANKK"
BASE COUNT         5153 a         2937 c         2123 g         4900 t
ORIGIN      
        1 tccttttttt aattactttt attaaaacca cataatttcc gatacaattt attttgcaaa
       61 taaatttttt tgtttttcca tcgtctaaaa ataccaggta tctgactact gataaattaa
      121 aatagaatcc ccttcattaa tatgttcaaa ccccttttta gatctgtatt cttagttaat
      181 cattcatcta aaattgcata taataacaaa tcatgatttc atccgaatgt gacacaatag
      241 cttatttact aataaaactg ttgtacatta tatctcttgc ctccgtgata ttatggtaag
      301 taaatcttac acacaagaaa aaaaatagta aataaaaccg gtataagtcg acaaaatctt
      361 gtagaagagg gatattttga tatctaaaac ttaaaaaatt atttgttgat tttcggatta
      421 tcaggttatg ctcaatttct tttataatct atgcacaagc ataaataaaa agctcagcat
      481 ttaagccaag caatgttgtt gatgaagcta tccaaaaggt gcatttaagg tgcagcttcc
      541 actgtttttt tagcttctgc ttttttgtta gaatccccta agtgcttcat gtgagggaaa
      601 acaagaacca cttcattcta aaaatataaa tataatgtca tttgcagtga caaactaata
      661 ttccaattat tccatttatc atctctaaga aaactaaaat ctgatttact taaaagtgac
      721 aaatctagga tctaaaaagt gctcactcat atgcgcctat attaaaggag cacaatataa
      781 aaaagatgaa attcattcat caaatcaaga tacgctaagc atgaaaccaa ctagcaaagc
      841 tttttccaat tctaacatac tttaaacact ttttaagacg catcattatt gaattagtat
      901 aatttatagc atcaactata attggccgca taacagcatt taagctgtta ttttcgcaat
      961 tgtttttacc acttttttta aaggttttca aaaaggtttt caaaactatc tataaataga
     1021 aaaaaagccc ttatataagg gctttattca caagatggcg tcgacggagg gattcgaacc
     1081 cccgaccgtg cgcttagaag gcgcatgctc tatccggctg agctacgtcg acaacgcata
     1141 tattttataa cataatcaaa atatatgcaa gcttaaattt aaaatttagt ctaactcaaa
     1201 agcacctgta tacaattgat agtatttacc cttttgtttc aacaagtcct catgattacc
     1261 acgctcaata atacgacctt gttctaatac catgatcgca tcactatttt gaatcgttga
     1321 aagtctatgt gcaatcacaa ataccgttct tccttccatc aacttatcca taccttcctg
     1381 aacaatacgc tccgtatgtg tatcaatgga acttgttgct tcatccaaaa tcaatacagg
     1441 aggattggca accgctgcac gagcaataga taaaagttgt ctttgtcctt gcgataaaga
     1501 ttctccacct gcatgaatca ttgtgtcata gccatgttct aagtgtttaa taaattcatg
     1561 cgcattcgaa agtttacttg ccgcaataca ctcttcatca cttgcatcta aacaaccata
     1621 acgaatatta tccataatgg taccggtaaa taagtttgta tcctgcaata caatacctaa
     1681 tgattttcta agatctgctt tcttgattaa ttttacatca ataccatcat atgtaataga
     1741 tccagattga atatcataaa aacgatttat tagattcgta atggttgttt taccagcacc
     1801 tgtagctcct acaaaggcaa ttttttgacc tggtttcgca aatagagaaa catcaaataa
     1861 aatctgttta tctggattat atgagaagtt tacattttca aaacgaacat ctccacgaag
     1921 tggaaccaat tcatcttgtc cattttttct tggatgatgc caagcccaat gatcatgatc
     1981 aatttgttct aaagtaacca caccatcatc cacttcactt tctgtatcga tgatttcata
     2041 aatacgctta gcaccagcat ccgccatgat taccgcattt aactgcatac taatttgtcc
     2101 aattggattt gtaaaactac gattcaactg taagaatgcc gcaataccac ctaatgttaa
     2161 gccagcaata tgatttagcg caaaaataga acccataact gctataagca cataagaaat
     2221 atatccaatg ttcattacga ctggcattaa aacgttcgca aacgtattcg cattcgcact
     2281 tgcttcaaac aacgcatcat ttacttgatc aaaatctttg atgctttgtt cttcatgcgt
     2341 aaaaacttta atgactttac taccttccat catttcttca ataaaaccat tttctatacc
     2401 taattgtttt tgttgagcaa taaagtattt acctgacttt gctgaaatct tttttgaagt
     2461 atacaccatc aataaaccac atgcgattgt taaaacagcc aatggaacat ttaagataaa
     2521 catagaaatc gtaactgaga taattgtcat taaagataca ataacctgag gaatactttg
     2581 agaaattagc tgtcttaaag tatccgtatc atttgtatac actgacatga tatcaccatg
     2641 tgcatgtgta tcaaaaaaac gtaatggcaa cgtctgcata tgttcaaaca attcatcacg
     2701 aatatgtttt aaagtaccca atgaaattgt taccattata cgattaaatc catacgtagc
     2761 taagatacct acaccataaa tcatcaccaa tttcattaat gcatttaata aaggcacata
     2821 attgggaact gtttgtgata ataatggaac aatataatca tcaatcaagc tctttaaaaa
     2881 catacttcca tatacattgg caatacttga cactataatt aatacaagca caataaaact
     2941 ttcaatttta taagaagtga ttgtttcttt aattacacgc tttaaactat tcgtacttct
     3001 attgttcatc aaaatcacct ccattgtttt gtgcttcaaa agtttcttta tacatcacat
     3061 tcgactttaa taattcttca tgcgtaccta aaccagaaat ttttccatcg tcaatcacaa
     3121 gaatctgatc tgcatcttga atacttgaaa cacgctgtga aataatgatt cgagttgtat
     3181 gtggaatctt ctctaagaat gcttgtcgaa tcaaagcatc tgtttttgta tccacggcac
     3241 ttgtagaatc atctaaaatc aaaattttcg gactcttcaa tatagctctt gcgatacaca
     3301 aacgctgtct ttgtccaccc gaaacattcg atccaccttg ctcaatatac gtatcatatt
     3361 tatttggcat caattgaata aattcatctg cctgcgctaa tttacaagcc tccatgatct
     3421 catcatcagt cgcatttgga tttccccaac gcatattctc tttgattgtt cccgaaaata
     3481 aaacattctt ttgtagaacc attgccacat tatcacgcaa tacttttaaa tcataatcac
     3541 gaacatccat tcccgctact ttaacactcc cttgtgttac atcatataat cgtggaatca
     3601 attgtactaa actcgattta ctcgatcctg ttgctccaag aattcctaga gttgatcctg
     3661 aaggaatgtg taagttgata tgacttaaaa tgtcttcatc atttccataa tggaatgaaa
     3721 catcctcaaa atcaatggtt ccattctcta ccgtttgaat tccattttca ggacttgtta
     3781 aatcagattc ttgatttaat acttcagcaa ttcgttttcc gctcgcaatc gacattgtca
     3841 ccatgacaaa aaccatactc atcatcatta gagacatcaa aatacttgcg gtatatgtaa
     3901 ataaagacat caattctcca gttgtcatat ttgaagctac gatttgatga gcccctaacc
     3961 atgaaatcaa aagaattgtg ccatacatac aaaactgcat aacaggagaa ttaaacacca
     4021 agattttttc agctttccct tgaatactaa aaatagtctc actttcttta cggaattttt
     4081 caatttcatg atccttacga acaaatgact tcacaacacg aatccctaaa atgttttctt
     4141 gaatactcgc atttaaaaaa tcataacgag agaaaagtcg actaaagatg ggacttacga
     4201 taaaaataat tccaaataaa acaacgccta aaacaagtat ggcagccaca aaaatcatag
     4261 ccaattcttt attaatgata aaagccatga ccaaagatgc gataagcatc aatggggcac
     4321 gaacacaaac acgaatcatc atctgatacg cattttgaat atttgtcaca tccgtcatca
     4381 tacgagtaat caatccgctt gttgaataag catcaacatt tgaaaatgaa aactcttgaa
     4441 ttttgcgata ttccgctttt cttaaattct tcgcaaaacc actcgatgcg attgccgcat
     4501 gctttccact tttagcacct gcccacaaag atacaaaggc tgccaccaac atcaaaaagc
     4561 cataaaacca aattgatttc atatcccctt tttcaatgcc attatcaact aggattgcca
     4621 ttaaaaaagg aatcaagact tcaagtatag cttcaattga aacataaaca ggacttaaaa
     4681 gtgtatcctt tttatattcc tttacttcct tcaataaagt tttaatcata aaaaccccct
     4741 tattctaaat tttgagcaag ttcctttaaa atctctaata attgttttcg tttctctatg
     4801 cctaaagcat tttcaaactt agattccatg ataaaaatct gcttttcaat atcctcatga
     4861 agatcctgct ctttttgtgt taatacaatt gtacggactc gtttatcttt aggattatgt
     4921 tttctttcaa taaacccctt ttgttctaaa cgatttagaa ttcctgtaac cgttggattt
     4981 gaaatatgaa aatgctcttc aatatctttc tgaatcactg gatccttatc tgtaaaatgc
     5041 aaatatccta aaacgtcgcg ctgggatttt gtcaaatcat acttctttaa tccttcattc
     5101 atatgtttgt ccatcttttt cttgatcaaa ctaaaataaa aaccaatctc atcattatca
     5161 cgcacagtat tcgcctcatt tccatagcat gctatatatt tatatagtac gctatatatt
     5221 ttactcaaac aattccattt gtcaattttt aattttttag ttatcatact tatgtatgaa
     5281 aaaaaatgaa gaatatatag taacatgtat cgatgataca gatttaaata gtggtgttgt
     5341 taaaattgac gatatggtcg tctttgttcc aaacttatta attggtgaaa aggcaaaaat
     5401 taaaattgtt aaggtcaaca aaaaatacgc ttttggtatt attattgaac ttttaaagcc
     5461 aagtataaac cgtcaagaac taacttgtga agttgccaaa acatgtggtg gatgccaact
     5521 acaacatatg agttatgctt atcaattaga ctataaatat aaacatgtta aaaaactttt
     5581 tgaagacata gaagtaaagc caattctagg aatggataat ccttggtact atcgtaacaa
     5641 agcacaattt cctgttaaaa ttaaggatgg aaaagtacaa atgggatttt atcgtcaaca
     5701 ttcaaacgac gttgtgacat gcaaagaatg taagattcaa gaccatgtga tcaataaagt
     5761 atttagcttt attcaaaaaa agatcacact taaaatggct caagatttaa gacacgtatt
     5821 tattcgccac tcaagtacag atgaaattca agtcgtgttt atcggtaaaa acgaaaaaaa
     5881 tattatatct ttatctgacc aattaaaaaa cacattcgca aatatcacaa gtattgtatt
     5941 taactacaac acacgaaatg acaatgtnnn nnnnnnnata taaagtgtta tatggtaatg
     6001 actccattat tgaagagtgt ttaggtaata aagtaaaatt acactttaaa gcattcttcc
     6061 aagtcaatcc taaacaaatg gaagttctat atagccaagc cattaaagcc gctaatcttt
     6121 ctggaaatga aacatgcatt gacatgtatg ctggaactgg aactattgga atggctgtaa
     6181 gtcaacatgc caaaagtgtg attggcgttg aaatcgttaa agaagctgta gataatgcca
     6241 atgacaatac caaaatgaat catcttgatc attgtcacta cgtttgccag gatgccacag
     6301 attttgcaca tgatcataaa gaggataaaa ttgatgttat ttttattgat ccaccaagaa
     6361 aaggcatgac tgaaaatggt attaaagaca caataacaat gcaacctgat aaaatcgtat
     6421 atgtttcttg taatcctaaa acattatcaa gagatttaaa aatatttaaa gaatatggat
     6481 atcaatgcga atacgtacaa cccgttgaca tgttttgtca aactattgga cttgaatgtg
     6541 ttgccaagct gacaaaaata gtgtaatatt ttaacactat gaggccgtgg tggaattggt
     6601 agacacgcta ctttgagggg gtagtgagta atgctcgtgt gcgttcaaat cgcatcggcc
     6661 tcaccatata gatatgaaaa gtgcttcggc acttttttat tttgcacaaa aaaaactaga
     6721 ccgcaatggc ctagttgtct ttacttggaa atttaaatac ttgctccccc tcttttgtca
     6781 ctaagtattt tccgcgcgca atatattcaa cataatctgg atttgtcaaa ttttgttttg
     6841 ccttctccaa ttccttcttt tttgaagaca aagctttttt ctctttctgc ttttgattta
     6901 tactagtcct taactcatgt gtcatggcca attgacgcat tccacccatg attaaaaaag
     6961 cagacattcc aagcattacg aaatatacaa atacatgttg aatctttttc cgtcttcctt
     7021 tcataaataa atcccctttt taatatagca catttttgaa agcgaattca atgaaaatta
     7081 tttcacacgc atagctaaaa tcgctatatc actcggatta atacccgaaa tacgactagc
     7141 ctgtcccaat gtcaaaggtc gaatttccgt taatttctgt cttgcctcta aagaaagatt
     7201 atccatattc tcataatcca aatcatgagc aagcttcatt ttttccattt gttgaagatg
     7261 ctttgcatca cgctttgcct ttgcgatata tccagcatac tttgtttgaa tctcaatttg
     7321 ttcattgatt tgatcttcat aatctaatcc tgtatattta gccaatccct ttaccgtgat
     7381 ttttggacgt ttaatcaaat ccaatgcact acatccatgt gctaatggtt caaatcctaa
     7441 actctttaga tattcatcta catcactatt tggtttaata tgcgcatttg aaagttcttc
     7501 tctagccact tcaatagctt ccattttctt tttaaacgca tcatatcttt cttgtgaaac
     7561 caaaccaatt tcataacctt tttctagtaa tcgttgatcc gcattatcat gacgcaacaa
     7621 caatcgatat tcagcacgtg aagtcaataa acgatatggt tctttcgtac cttttgtaca
     7681 taaatcatct aacataacac caatatacgc ttcatcacga cgaaggacaa atggctcttt
     7741 tccatccact ttcaaagctg catttgcacc agccattaaa ccttgtccag ctgcttcttc
     7801 ataaccactt gtaccattga cttgtcctgc cgtaaataag ttttcaacaa ttttactttc
     7861 catgtttggc ttcatttgaa gtggatcaat cgcatcatac tcaatcgcat acgcatattt
     7921 ttcaatcaca caatgctcta agccaggtaa tgaatgaacc atttcttctt gtacatcaat
     7981 tggcatagat gttgaaaaac cttgaatata aatcgtatcc aattcttttg attccggttc
     8041 taagaaaagc tggtgtcttt ccttatccgc aaaacgaact aatttatcct caatactcgg
     8101 acaataacga ggccctacac ctttaaccaa tcctgaatac atggcagaat catgtaaatg
     8161 agtatgaata atttcatgcg tttttggttg tgtataaatc aaatgacaaa tctcttgttt
     8221 ttcaaaaggc aatacgtctt ctgggtttgt tgtctcacta aaacgtaaaa actgatttgt
     8281 ccccggctga ggttcgccct ttgaaaaatc aatggtatcc atttttacac gtgctggagt
     8341 ccccgttttt aaacggaatg tacgaattcc tgcatcacgc aaagactttg ataaagtatt
     8401 cactgttctt tgttcttctg gaccagaaac agttgacgta tgtcctctta agacattact
     8461 tgtcatatat gtacccgctg tcaagacaac ggctttactt tctataaaag ttccatcttc
     8521 taattcaaca ccaatggctt taccatcttt aataaccaca cttttacatg cagcctcaat
     8581 aatatctaaa tgatcttgtt tttctaaagc ctctttcata aaatgtttat acgcgatctt
     8641 atcactttga acacgcaaac tccatactcc tgggcctttt gtcgtattca acattttaaa
     8701 ttgtaaagcg gttttatccg ccgcaatagg catcatacca cccaacgcat caatttcacg
     8761 tacaacaatt ccttttgccg gtccaccaac acttggatta catggcatag aaccaatcat
     8821 atccttactt agagtaacaa ttgctgtttt tttattcatt cgactcgatg ctaaacatgc
     8881 ctcaattcca gcatgtccag ctccaattac tataacatca tacattttat acacccgatt
     8941 tctttcttac ttgaaagtga aaaatacttt gaggaacttt taaatataaa gttacaacta
     9001 catatacaat tacacttaat aaaccattta aacacatttt gaataaacat actagttttg
     9061 aaccatcaac tccatataat cctaatttgg ataatagtat agaagtaatc cacaatgcga
     9121 gtaagccaac taaaatacgt actgaaataa tcattgtttt acgataagaa atatgataca
     9181 catcatgaat ttcttttaaa tttttataaa gaatataacc cgtacctaat aagcttgaaa
     9241 taacagcacc cgcaataccc atgatccata caaatggaat caataataca cctttgatgc
     9301 atacaccaat ggctagattc tttaagacat tttttcttaa ttccaatgac accattaaat
     9361 ttgtcaccaa aggagcaaat gtagataaga atccttctaa agccatccat tgcacaacaa
     9421 atgtacaaag ttctaaattc tctgaataat acaatgtata attcaatggt cctgcatacg
     9481 caaagataca gaaagatact ggaattgcga cataaagtac gacattaata ctatccaata
     9541 cattcttctt aacaagttta taatttttct tagataaagc ttctgaaata tgtggaataa
     9601 tagctgtcgc aaaaccagga cccaaaatca ttggaatcgc atttaatttt ccagcaacat
     9661 agttcgatgc agaaataaca gttgtggtat ctttcgcatt gtaatgcatc tttaacccag
     9721 ttggtagtaa aaatccatta aacacctggt caatatttcc tagaatcgca acaaccaaat
     9781 atggaacggc taaaataaga atttctctaa agatcttttt agctgaatga caagcctttg
     9841 tctgtttact tgccatttta tcaaccacat attgatgttt acgatcaaaa tacgcaaatt
     9901 gtgcaattgc cgcaattgtt gccacagaag ttgacattac agctgcatat aatgcatatt
     9961 ttttatccca tccaaacgca tatacaacta aacaagccgc acttaataaa aaagctacac
    10021 ggaatacttg ttcaaaagct tgagaaaatg catattcctc catttctttc aatccttgat
    10081 aatatcctct aaaagcggat aaaacgggaa caagaaaaat agctaaagat aatattttta
    10141 agcaatttgc catgatatcc gcattcttgg ctaccatagc tggcgcaatg acattggata
    10201 aagtaaataa tagaatcatt cctacaaatc ctgttaatcc aagtatcgca aatgcaattt
    10261 ttttaactaa aataacactt ttagcatctt ccaatgcgat atagcgtgct actaaagttg
    10321 caaccgcaaa aggaacaccc gattgaaata tagtcaataa ataagcatat atattataag
    10381 attgtgcata tacagattga tatgcttctg attgcagtat atatgaaaat ggaattgtat
    10441 atgccaatcc aattaatttt gaaacaaaca gaccacccgt accaatcagg ccgccaacaa
    10501 taatcgactt tttagccgct gaagattttg acataaaata aaatacctcc aatttcataa
    10561 ttataacata catctataaa agtattatca ttttttaatc attaagagta tagtaaatat
    10621 gaagaaggtg aaattatgta tcctgcctat atccatacac aagctacggc tcgaaacatc
    10681 gatcatgccg ctatagagac atatcaaatc ccaagtctga ttcttatgga acatgcagct
    10741 atagaatcga ctaaaatcat tgaaaaacat ataactattg ataaatcaat ttgtattctt
    10801 tgtggacctg gaaacaatgg tgcggatgga ttagctatcg caagactttt acaaactaaa
    10861 tattcaaaat tatttgtagt tgttccagaa gtcaaaaaga tgtctgagga agaaaaaatt
    10921 caatttcaaa tgattcaaaa tctcaatatt gaatatgact ataacctacc aaatgaagca
    10981 tatgaagttt atattgactg catctttggc aacggattat caagagacat cacaggtttt
    11041 tataaagcga tgattcaaaa tgtaaacgca acacattcaa ctatcatcag tattgatatg
    11101 cctagtggca ttgatgccac gatgggcaat attctaggtt gtgctattca tgcagattac
    11161 acaatcagtt tagattgcta taaacaagga caatggttaa acgatggtaa aagtcattgt
    11221 ggaaaattat atcttgtaga tattggcatt ccgcaaattc tacatcaaaa atgcatggac
    11281 ataataaaag tcttgaacaa agaggatatc catatcccaa atcgtccatt aacggctcat
    11341 aagtcaactt ttggcaaagc cttgatgatt ggaggaagcc aaaacatgca tggagccata
    11401 catatggcaa gcttgagtgc ttataaaagt ggtattggaa cactcacatt aatggttcca
    11461 gaatgtattt cgaatattct tgcgatcaaa tctgattttt atatgcttct acaatgtagt
    11521 gatcgtaatg gaacctttag ttcgaaagct atcgaagttt tgaaacaaaa tatgaataac
    11581 tattccatac tttcaatcgg caatggtatg caaaaaaata atgttacaat atctttagtg
    11641 gaacaagtat taaagagtga taaaaaagtt gttcttgatg cggatgcctt atgggctgcg
    11701 caaaaaaata tggatttgtt aaaacgaaac gaaacaacaa tcttaactcc gcatattaaa
    11761 gaaatgtcgg acttaacagg tatcaacata acaacaatct tatcaaaccc atttgaaatt
    11821 gcacgtgact tttctaaaga atatccaagc tgtgtactga tactaaaatc aagtcaaact
    11881 tatattgccc atcaaggaaa tgtatatgtt ttagatgcac aaaacgctgg tcttgccaaa
    11941 ggtggcagcg gagatatatt gtgcggtata gttgtggcaa tgctaggtca atgcaaagat
    12001 ccaatgcaag cggctctatg tgctacctat cttcattcgc aaagtgctaa actcgatata
    12061 gacttagcga gtttcatgcc agaagatatt atcaacaaca ttccaaacac attaaaaagg
    12121 ctgagatcct agtgattctc agcctcatct ttttctatta aaatttttat agtttgaact
    12181 gcacattcca tcaatggttt taaatcatga ttttgaatgt ttgataatcc cgctttttct
    12241 ctttcctgat tagccaaaca taaaaacata gctccgctac gaacatgtcg agtttgactt
    12301 acaataaaga gagtgctcga ttccatttcg gatgctaaac aatctagctt acaataagca
    12361 ttccatttgt tttgcaattc cggataaact ggcatcgttt caggcgcatg ctgaccaaag
    12421 aaagaatcct tactttgaac aacacctaca tgagaaggaa gacaaagttt ttttgcactt
    12481 tcaacttgtg cacttaatac atcaaaactt ggaacagctg gaaattcgat gggtgcatat
    12541 tcacgagttg taccttcttg gcgaatagca ccactcgcaa tcacgatatc gccaccttga
    12601 acatcaagtc gtataccacc acaagtacct acccgtaaga atgtatgaca tccaagtcgt
    12661 acaagttctt caattgcaat tgcagtacta gctccaccaa taccagttga aacaacactg
    12721 cataatatcc cattcatata tcctgaatat gtaacatatt cacggttatc cgcaacaaaa
    12781 taagaatctt ctaaatacga tgcgatatct tttactcttt ttggatcacc cgtcaaaatg
    12841 acataatcac caacatcatt tacagataat tgagtatgat attgtttatt tgaaccttct
    12901 gtgtaatcaa tcataaaata tcctagaaag attggaattg taagtagtag acatttggat
    12961 gaaacattgt cgtattaaag ctataagctt gacaatcatc tttttctcca tcccaatcat
    13021 tgttaagttt agataaaaga tcatcaattt cattttcttc taattcactt gtcgcataga
    13081 attccataca acctgctaaa cttggctgta atcgacttgg agaataagac atttttgaac
    13141 aaatttcatt caatgcattt tgaacaagtt caacaatcgc atcatcttct ttatctacaa
    13201 caacatgtaa tcttatcttc attaagcatt cacctgatca ataacatatt tacataattt
    13261 ttttaagttt gtatcgttag ataaagtaat aactttaccc aaacttaaat tcaatgctgc
    13321 aataacgggt tccccattat ccatcaatac atttaaagta cctgtggcgt tttcataata
    13381 tacatcaaac aaataacttt gttttccgtt atcttctttc ttttctctta aatcaaaatg
    13441 aaaagcatcc ttattcttta aaataatacg aacagctcca gctccagagc tagggattga
    13501 ttttgtcata aaagcctcct actttttatt agcatcataa gtaagcataa tgcctaaatt
    13561 cgaataaatg cttaaatcta catccgcaac atcacaagtc acatgagcct gcataccttt
    13621 caatttaggt aacatagcta atgctttctt tgcatcttca ctatgtaaag ctgaaacgga
    13681 taatgcgatt aaaatttcat ttgaatgtaa tctagggttg actgaaccta aatatgctgt
    13741 ttttagattt tgaataggca caatggcttc aggagaaatc aacactttat ttttatcaat
    13801 acccgctaga tattttaata cattcattaa tacagacgaa catgcaccca ttaggtttgt
    13861 cgttttacct gtaataattg ttccatcatc taattcaatt gcacctgaaa aagaatgaga
    13921 atcttcttct tttttacgtg cagccataac acacggacga tctagttctg taattccagc
    13981 tttattcatt acgacttctt gtttttgaac ttcttcatgc gaacaaagtt catgtgcata
    14041 acgagtctta cttacaaaat aacgacgaat aatttcttga ttactagcat tacaacaagc
    14101 atcatcatca ctaatacaaa gtccagccat attaacaccc atatcggttg gactttgata
    14161 aggacttgaa ccataaattt cttcaaaaat gttttttaat actgggaaaa tttcaatgtc
    14221 acgattgtag tttactgtaa cttcattgta agcctgtaaa tgaaatgggt caatcatatt
    14281 tacatccgca agatctgcag tagctgcttc ataagcaaga tttacaggat gattcaaagg
    14341 aagattccaa attgggaatg tttcatattt tgcatatcct gctttaatac catgtttatg
    14401 ttcgtgatat aactgtgata aacatgtagc catttttcca ctaccaggac caggagctgt
    14461 tacaacaact aatggacgag tcgttttaac atagtcattt tttccaaatc cagaatcaga
    14521 aataattttt ttcgtatctg taggatatcc ttcaatataa taatggaaat atacatcgat
    14581 ttttctacgt tttaaacgcg ctttaaataa atctaccgct ttttgacgat tgtattgtgt
    14641 cacaacaaca ctaccaacat acaatccacg cttagtaaat tcattgatca aacgtaaaac
    14701 atcttcttga tatgtaattt gaagatctga acgcgtttta ttttgttcga tatcatttgc
    14761 gctaataaca atgacaattt ctgcatcatc ctttaactgc atcaacatct gtagttttga
    14821 atctggtgaa aatccaggta aaacacgaga tgcgtgataa tcatcaaata atttaccacc
    14881 aaattccaaa tataatttat ctccgaattg agcaatacgc tccataatat gcttagattg
    14941 catttctaaa tatttttgat tattaaatcc aattttgttc atattttttt actctccata
    15001 caacgcatta ttgtacaata aaatataaaa aaaggcaaaa aaaaaggacc cggcagctag
    15061 ctatcttcgc aggggcaagc ccaactattg tcgccgtgaa agtgcttaac ttctgagttc
    15121 ggg
//
LOCUS       DBMDWL010000033        14743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1380_length_14743_cov_2.882393, whole genome shotgun sequence.
ACCESSION   DBMDWL010000033 DBMDWL010000000
VERSION     DBMDWL010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_14743_cov_2.882393"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="ACZPDN_03675"
     CDS             <1..317
                     /locus_tag="ACZPDN_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR1297753.1"
                     /translation="MQDTLFQLCKGHYCDFGGSTFKDCMFDINDLTGSGFVQCDFKKT
                     LFDKCILNSTEWFNTKLKELDFSSCEIENIAVSSDKLTGVVVNSSQALEFVKLLGIVV
                     KD"
     gene            complement(307..837)
                     /locus_tag="ACZPDN_03680"
     CDS             complement(307..837)
                     /locus_tag="ACZPDN_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1297754.1"
                     /translation="MNDTKLTIANALLILLKTQPLNKITVTQITKEANLTRQTFYRNF
                     EDKEDLVNWYFEKLCLDSFKEMADQTTLKKALIKKFTFIQSQNTFFKEAFKEDDYNSL
                     TNYDYRCIYDFYKKKIETKTTIDSQLDFLLQMYCHGSIEMTKSWVEKNMYLDIETLVN
                     MLIESMPERLKQYINL"
     gene            919..1290
                     /locus_tag="ACZPDN_03685"
     CDS             919..1290
                     /locus_tag="ACZPDN_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR1297755.1"
                     /translation="MQLGESMEDYLEAILVLSKCLDHVRSVDVASYLGFSKPSVSHAV
                     KLLSEQGLIVLDVSKFLTLSEEGKRIAEETYARHTFFSEMLQNIGVPKNIATEDACRI
                     EHVISQETFEAIKKYYQNNED"
     gene            1335..3500
                     /locus_tag="ACZPDN_03690"
     CDS             1335..3500
                     /locus_tag="ACZPDN_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013316.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1297756.1"
                     /translation="MRPEILAPVGSMEALNAALAAGCDAVYFGLPSFGARAFANNFDL
                     QTTKEVIERCHLCGVKVYITMNTILYEDEMEDAYNMAKMVHLFGVDALIIQDLGFIHL
                     LHHRLPNLVLHASTQLSVSNPYQIEQLKKLGVQRVVLARECTKEEVQACVDTGIEVEV
                     FVHGALCICYSGQCYFSSVHYGRSGNRGMCAQPCRMKYSLYEDGNKVDTQGDYVLSPK
                     DLSLIDEVNTLADMGVCSLKIEGRMKSAPYVYESVSQVKKVLENMERSHLDEQNLRVT
                     FNREYTLGHMYGASGKELMNSKTCNHQGIEIGRVVKVNKNKICIQLSQDLHQNDGIRF
                     ERENLGCHVNFMYDKKQKLISFMPKNNTVLIEGPVGVHVGSIVRKTMDSELNKTIEEK
                     IRTSNRQSIVNAVVSCSAVGKPMVMEVYNDSTRVSVSTDINSVKALKRATDEETLNKQ
                     FSKTKDSWASFGRIEYHLGKDIYFPISVMNQLRRDALEKMKKACLKQEAIVEKEYSYV
                     PTESKTAFDLFEINSMNQKVDDSSSYVSEMLNNENVINKSNITGVDGFVNAHLGKGKI
                     VDSLNISNSYGVAAILEMGYESCVLSDECDKYQVEALMKAFLNRYHFDAPVYKTLYQK
                     RRLMTMKHCPVNTALKDGKRVGCGLCHSHRYELEGLDGKRVFLLGDKDCHMRLYDVNI
                     MDEIENRKEYESYGIKHFRFVFTDENQEEVKTAFKAYNR"
     gene            3563..4891
                     /gene="eno"
                     /locus_tag="ACZPDN_03695"
     CDS             3563..4891
                     /gene="eno"
                     /locus_tag="ACZPDN_03695"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1297757.1"
                     /translation="MPIIVDVYAREVLDSRGNPTVEVEVTTESGAYGRAMVPSGASTG
                     VREALELRDGDKARYLGKGTTKAVENVNTKIAPKIIGMDCRDQVLIDKTMLELDGDPF
                     KKNLGANATLGVSMACALAAADFYGEPLYKYFGGFNGKTLPVPMCNVINGGSHADSTV
                     DFQEFMIMPVGAKSEKEAVRMAAETFHALKSVLKKKGYNTNVGDEGGFAPSCKDGNEE
                     PLELLVEAMEAAGYVPGKDMKIAMDVAASEFHNTETGLYELKKSGQGTKTSDEMIDMY
                     EEWIAKYPIISIEDGLGESDWDGWKKLTDRLGDKVQLVGDDLFVTNPSILYEGIEKGI
                     ANAILIKVNQIGTLTETFDAIEMAKKHGYTCIVSHRSGETEDTTIADIAVGLNAGQIK
                     TGSLSRTDRIAKYNRLMRIEDELNQRGTVNVAEYLGDKTFYNLPAVEFKK"
     gene            4987..5793
                     /locus_tag="ACZPDN_03700"
     CDS             4987..5793
                     /locus_tag="ACZPDN_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1297758.1"
                     /translation="MVRTIVCDLDGSLMNPSSGIYVKEAVKEALIEVEKRDVLVVLNS
                     ARCFQGVYPLSKQILMQDFGGYVISCNGAHCFDVKMKKTIFEYVISNNDVSLIWQYAQ
                     KHNLGVGYSQPDYFVANKMTYGFELDMHNCDVDYILTNHMEKYLKNSVWKVSISDSKE
                     RIDSIYDEMKSRIESNCNVRVVHSTDTMIDIIHKDCEKLNSVNRLLEKINISWQHVSS
                     IGDGSSDAPVLEASGLGVTLENGCEACKKVANKIVPSCYEDGCIEWLKEL"
     gene            5796..6788
                     /locus_tag="ACZPDN_03705"
     CDS             5796..6788
                     /locus_tag="ACZPDN_03705"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR1297759.1"
                     /translation="MRVVDMHCDTILELLSRDLKGKEASLYSNDLSIDLEKMKKGNYL
                     LQNFALFTDQKELAIPEQQTMRLYDEYCTQMDKYADYIAPVYTFSDIEKNDKEGKLSS
                     LLTLEDGGVCFSDLAMLRNYYRMGVRMIALTWNYENGIGYPNLSMKDMDGNRPNYKRS
                     VDTERGLTEFGISYVKEMERLGMIIDVSHLNDAGFYDVLKYTTKPFVASHSNARSVCN
                     VARNMSDDMILQLAKRGGVMGLNFCGSFLADRDDHKSCVDDMVKHILYIKDLAGIDVI
                     GLGTDFDGISCPLEIENASMMNLLEEALYKAGLTQEEVEKIFYKNVLRVYKSVL"
     gene            6887..7558
                     /locus_tag="ACZPDN_03710"
     CDS             6887..7558
                     /locus_tag="ACZPDN_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HIR1297760.1"
                     /translation="MELLKLLGILIVVIGFVLKLDSILIIMIAAIVTALVAGMDPVTF
                     LETLGSSFVANRSMCVFVMVMVVTGTLERNGLKQAAANLMMKFKNASAGVVLAIYGVF
                     RLVFAAFNVSFGGVAGFVRPIVLPMASAAAKKNGKKISEKYEENLKGMASGMENIAWF
                     FGQVLFVGTSGMILVQSTLADLGYNVELVDLMKVQIPVAIIAVGVAIVYYYLKDKQLS
                     RKETK"
     gene            7558..8511
                     /locus_tag="ACZPDN_03715"
     CDS             7558..8511
                     /locus_tag="ACZPDN_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HIR1297761.1"
                     /translation="MNPIQFYTSVDVTLSDKLLEVMYCIIGLISMYVAFKNLKDKENK
                     NSVGSFVFWFDLGVMFVLGKWLPALVDGILLIILVLPPILKKVSPGNEPEPTLEEMEQ
                     NNKKIGAKVFVPAVCIGLFALLAAFFTKISPLVGMSVGVFVAMIILRIYSKANTPSVF
                     LKDCRRMMDVVGPLSMLPMLLAALGAVFTAAGVGDVISGLVSNIIPAGNVTIGIIVYA
                     IGMAVFTMIMGNAFAAITVMTVGIGAPFVLKYGADPVVIGSLALTCGYCGTLCTPMAA
                     NFNIVPVAILEMKDKNGVIKKQVLVAVVMLVVQIVMMIMMS"
     gene            8525..9172
                     /gene="pcp"
                     /locus_tag="ACZPDN_03720"
     CDS             8525..9172
                     /gene="pcp"
                     /locus_tag="ACZPDN_03720"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIR1297762.1"
                     /translation="MKKVLITGFDPFGGEKINPAYEAVKLLPDEISGAKIIKKEIPTV
                     FQKGPEAVYEEIQSSKPDYVLCIGQAGGRSQVTPEWVGINFRNARIADNEGNQPLQTS
                     VVENGPEAYFTMLPVFRMVEKMKENGIPASVSYTAGTYVCNDVMYSVLHYCHTEFKDV
                     KGGFMHVPFATEQTVNQPAGTPGMNLKDIAKAIELSVEAMLESDTDIKVVSGETH"
     gene            9253..11031
                     /gene="pepF"
                     /locus_tag="ACZPDN_03725"
     CDS             9253..11031
                     /gene="pepF"
                     /locus_tag="ACZPDN_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIR1297763.1"
                     /translation="MKRNEQLVENTWKIEDIYPNLDVFYKELEEAKMQSKELLKYKDH
                     LTNASSLYAYLKGYEELNEKLYTLDCYTSLLSDQDGSNAFYQDLKGKANIVITEIHSQ
                     QAFFEVELLKLSDVETYFEKEPGLERYRRFIQDIYRLKPYTLSMEKEELLAGCQDIFS
                     SSERAFYILNDVDTRFEDVKDAKGNVLPLTSGSYVDYLESADETLRKSAFENMYAAYG
                     KVQNTMASLLNSQCKMLKYESDLRGYPTSLEASTNKNGVPSSVYKNLIKQVHKDIAPL
                     HKYMKVRKKALKKDKLNMYDLYVPLVKEFDKKITFETAKKEALASVALLGQEYVEAYE
                     KGLNSRWIDVYENEGKRSGAYSCGVPCHPFVLLNHTDTLRSEFTLAHEMGHAMHSYMS
                     FKHQPFINSDYKIFVAEVASTCNEALLMHYLLERTTDKKERAYLINYLLEQYRGTMYR
                     QAMLAEFEMLINEEVAKNNMLSAELLNKINHDLNAFYYGEDVIVDAPIDLEWAKIPHL
                     YMNFYLYQYATSFCASMAISQKLLKGNPEDIENYLNFLKGGCSKSPINLLKMAGVDMT
                     SPQPIHDAICQFDKLVDEFDELINCK"
     gene            11109..12803
                     /locus_tag="ACZPDN_03730"
     CDS             11109..12803
                     /locus_tag="ACZPDN_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1297764.1"
                     /translation="MNNKIMKSGLAAMMAASLVGCSSSSSDNSNNVLTVGITAQMNGV
                     FSPLYYESAYDAYAINLIYQSMLQYDENEELQPVLAKELPTISDDGKTVTFKLNKGVK
                     FSDGSKLTSSDVKYTFTVLADPSYTGRFSSDVENIEGYKEYHDGDATELSGIETPDDY
                     TVVFHMTQPRIDAVTTFGTRAICSDTQYKYKKGHISKIEKDTSNPIGSGAYKLNKYSK
                     ATGASFVRNENFKAKKGEYSIDKVIIKVCSTSTELNELQKGNVDYLPENADPTKIGQV
                     SLDDNLTFNTYPRATEGYVYFNCAQGPTADPAVRKALAYATDRQGFVDSFFSWEKASK
                     EAKKEKLAFVPAVYANPASANLGTYIRGEEALDGLETYEFNEDKANQILDEAGWEMAD
                     DGYRYKDGQKLRVRFMIAEGSSSLETLIPMIEKTYKDIGVDLKQTILEFNSLLSKTSD
                     DSALGEWEMSCLAMSFTGVANTDLNSMLKTGDVNNYARLSDSELDSLLDEAMYTTDEA
                     KSTELYKQIMIKENDLLPYLALYGNQNFNLYNKRVKNMKTGPIHNWSQAMDTATLD"
     regulatory      13086..13252
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            13335..14165
                     /locus_tag="ACZPDN_03735"
     CDS             13335..14165
                     /locus_tag="ACZPDN_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1297765.1"
                     /translation="MNFKKLMASAMVCAFALTGCGSNTATTSEDKDTFVVGMECGYAP
                     FNWQTSEKTDTSVKLGKAGYADGYDVQVAKKIAKKLGKKLVIKKTAWDGLIPALENDE
                     IDAIIAGMTKDKDREEGADFTTPYYDSKGMIMIVRKDSEEAGYTDIQQFTGKNIVGQK
                     ATNYDDVIDQIKGVNHVTPKATYPEMILALKQNEVDGITAEMAVAKGVVEANPDLTVV
                     QFADGHGFDCDTTVSIALKEGSRDSKFFKKVQKALDSISDEEREEMMEYAVEHQPTED
                     "
     gene            14168..>14743
                     /locus_tag="ACZPDN_03740"
     CDS             14168..>14743
                     /locus_tag="ACZPDN_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1297766.1"
                     /translation="MHIDIAKTWNIFVRNIPLFQYGIEMTLLFAFVGTFAGFVVGLLV
                     GGIRAIEINEYDSTAIKILKNIGRIITGLYIWVFRGTPMMVQAMFIYYAGKPVIGWTP
                     ITAGLIIISINTGAYMAEIIRSGIQSVDKGQNEAAMSLGMTTMQTLMYVVFPQAIKNS
                     FPSIGNQLIVNIKDSSMLNVITVTELYFQTTS"
BASE COUNT         4978 a         1863 c         3151 g         4751 t
ORIGIN      
        1 taatgcaaga tacattattt caattatgca aaggccatta ttgtgacttt ggtggcagta
       61 catttaaaga ttgtatgttt gacatcaatg atttgacagg ttcaggattt gttcaatgtg
      121 attttaaaaa gactttattt gataaatgta tattaaattc aacggaatgg tttaacacaa
      181 aattaaaaga gcttgatttc tcaagctgtg aaattgaaaa tattgcggta tctagtgata
      241 aattaacagg tgttgtcgtt aattcatctc aagcacttga atttgtgaaa ttactaggta
      301 ttgtagttaa agattgatat attgtttcaa tctttcgggc atggattcaa ttaacatatt
      361 gacgagtgtt tcaatatcta aatacatatt tttttctacc catgatttag tcatttcgat
      421 agatccatga caatacattt gaagtagaaa atcaagttgt gaatcaattg ttgtctttgt
      481 ttcaattttc tttttgtaga aatcatagat acaacgatag tcatagtttg tcaaagaatt
      541 gtagtcatct tctttaaagg cttctttaaa gaatgtgttt tgactttgaa taaaagtgaa
      601 tttcttaatt aaagcttttt ttaaagtggt ttgatccgcc atttctttaa aagagtctag
      661 gcatagtttt tcaaaatacc aattcactag atcttcttta tcttcaaaat tacgataaaa
      721 tgtttgtctt gtcagattcg cttcttttgt gatctgtgtg actgtaatct tgtttaatgg
      781 ttgtgtcttt aatagaatta atagcgcatt tgcgatcgtt agttttgtat cgttcataca
      841 actattatat gtcatgacaa ttgactttaa aaggggacta gcttaaaatt aaagaaaaga
      901 gttagaggag taatgaatat gcagttaggc gaatcgatgg aagattattt agaagccatt
      961 ttagttttat caaaatgttt agatcatgtt cgtagcgttg atgtcgcaag ttatttgggt
     1021 ttttctaagc ctagcgtctc acatgccgta aagttattgt ctgaacaagg attaattgtt
     1081 ttagatgtta gtaaattttt aactttgagt gaagaaggaa agagaatcgc agaagaaaca
     1141 tatgcgcgtc atactttctt ttcagaaatg ctacaaaata ttggagtacc taaaaatatc
     1201 gcgacagaag atgcttgtcg tattgaacat gtgatttcac aagagacttt tgaagcaatt
     1261 aaaaagtatt atcaaaataa tgaagactag ctatagattg tatctgtagc ttttattttg
     1321 aaaggagtaa gaaaatgaga ccagaaatat tagcacccgt aggaagtatg gaagcattaa
     1381 atgcagcatt agctgcagga tgtgatgctg tttattttgg attgccaagt tttggagcta
     1441 gggcattcgc gaataatttt gatttacaaa ccacaaaaga agttattgaa agatgtcatt
     1501 tgtgtggcgt aaaagtatat attacgatga atacaatatt atatgaggat gaaatggaag
     1561 atgcctacaa tatggcaaag atggttcatt tgtttggtgt agatgctttg attattcaag
     1621 atttaggctt tatccatcta ttacatcata gactaccaaa tttggtgcta catgcttcta
     1681 cacagctttc agtttctaat ccttatcaaa ttgaacaatt aaaaaaatta ggggttcaac
     1741 gtgttgtttt ggctagagaa tgtacaaagg aagaagttca agcctgtgtg gatacaggta
     1801 ttgaagtgga agtctttgtg catggagctt tatgtatttg ttattcaggg caatgttatt
     1861 tttcaagtgt acattatggg cgaagcggaa atcgtggcat gtgtgcgcaa ccatgtcgaa
     1921 tgaaatatag tctttatgaa gatggaaata aagtagatac acagggggat tatgtattaa
     1981 gtcctaaaga tctatctttg attgatgaag tgaatacact agcggatatg ggggtttgtt
     2041 cattaaaaat tgaaggacga atgaaaagtg ctccttatgt atatgagagt gtttcacaag
     2101 tcaaaaaggt attagagaat atggaacgta gtcatttgga tgaacaaaat ttgcgtgtta
     2161 cattcaatcg tgaatatact ttaggccata tgtatggggc tagtggcaaa gaattaatga
     2221 atagtaaaac ttgtaatcat cagggaattg aaattggtag agttgttaaa gtaaataaaa
     2281 ataagatttg tattcaatta agtcaagatc tgcatcaaaa tgatggaatt cgttttgaaa
     2341 gagagaactt gggttgtcat gtgaatttta tgtatgataa gaaacaaaag ttgatttctt
     2401 ttatgcctaa aaataataca gttctaatcg aagggcctgt aggggttcat gtaggtagta
     2461 ttgttcgtaa aacgatggat tctgaattga ataaaacaat tgaggaaaaa attcgtacat
     2521 cgaatcgtca aagcatagtc aatgcggttg tctcttgtag tgcagttgga aagccaatgg
     2581 tgatggaagt atataatgat tcgacaagag taagtgtttc tacggatatt aatagtgtta
     2641 aagctttaaa gcgtgcaacc gatgaagaaa cattgaataa acagttttct aagactaaag
     2701 atagttgggc aagttttggt cgaattgaat atcatttggg taaagacatt tattttccga
     2761 tttcagttat gaatcaactt cgtagggatg ctcttgagaa gatgaaaaag gcttgtttaa
     2821 aacaagaagc aattgttgaa aaagaatata gttatgttcc aactgaaagt aaaactgcat
     2881 ttgatttatt tgaaatcaat tcaatgaatc aaaaggttga tgattcttct agttatgtga
     2941 gtgaaatgtt aaataatgag aatgtaataa ataagtcgaa tattacaggc gttgatggat
     3001 ttgtgaatgc gcatctaggt aaaggaaaaa ttgtggattc attaaatatt tctaattctt
     3061 atggtgtggc tgctatttta gagatgggat atgaaagttg tgttttaagt gatgagtgcg
     3121 acaagtatca agttgaagca ttgatgaagg catttttgaa tcgttatcac tttgacgctc
     3181 cagtctataa gactttgtat cagaaacgtc gtttgatgac gatgaaacat tgtcctgtaa
     3241 atacggcttt aaaagatgga aaaagagttg gctgcggatt atgtcatagc catcgttatg
     3301 agcttgaagg tttagatgga aagcgtgtct ttttacttgg agataaagat tgtcatatgc
     3361 gattgtatga tgtgaatatt atggatgaaa ttgaaaatcg aaaagaatat gaatcatatg
     3421 gaatcaaaca tttccgtttt gtgtttactg atgagaacca agaggaagtt aaaaccgctt
     3481 tcaaggccta taatcgttga ttttagatgg cttgcgtgct attattttat tagcttaagg
     3541 cttagaggag gatattttaa ttatgcctat tattgttgat gtatatgctc gtgaagttct
     3601 agattcacgt ggaaatccaa ctgttgaagt tgaagtaact acagaatctg gtgcttacgg
     3661 acgtgctatg gtaccaagtg gtgcaagtac tggtgttcgt gaagctttag aattacgtga
     3721 tggagacaaa gctcgttact taggaaaagg aacaactaaa gctgttgaaa acgtaaacac
     3781 taaaattgct ccaaaaatta tcggtatgga ttgccgtgac caggtattaa ttgacaaaac
     3841 aatgttagaa ttggatggag acccattcaa gaaaaattta ggtgctaacg ctactttagg
     3901 tgtttctatg gcttgtgctt tagcagctgc tgatttctac ggagaacctt tatacaaata
     3961 cttcggtgga ttcaacggaa aaactttgcc agttcctatg tgcaacgtta tcaacggtgg
     4021 atcacacgct gactctacag tagatttcca agaattcatg attatgccag ttggtgctaa
     4081 gagtgaaaaa gaagctgttc gtatggctgc tgaaactttc cacgctttaa aatctgtatt
     4141 aaagaaaaaa ggatacaaca ctaacgttgg tgatgaaggt ggatttgctc caagctgtaa
     4201 agatggaaac gaagaacctc ttgaattatt agttgaagct atggaagctg ctggatatgt
     4261 tccaggaaaa gacatgaaga tcgcaatgga cgttgctgct agtgaattcc acaacactga
     4321 aactggatta tatgaattga aaaaatctgg tcaaggaact aagactagtg atgaaatgat
     4381 cgacatgtat gaagaatgga tcgctaaata cccaatcatc tctatcgaag atggattagg
     4441 agaaagcgac tgggacggat ggaaaaaatt aactgaccgt ctaggagaca aagtacaatt
     4501 agttggtgat gacttattcg taactaaccc ttcaatcctt tacgaaggaa tcgaaaaagg
     4561 aatcgctaat gcgatcttaa tcaaagttaa ccaaatcggt actttaactg aaacattcga
     4621 cgctatcgaa atggctaaga aacacggata cacttgcatc gtatctcacc gttcaggaga
     4681 aactgaagac actacaatcg ctgacatcgc tgttggattg aacgcaggac aaatcaagac
     4741 tggttcatta agccgtacag accgtattgc taaatacaac cgtttaatgc gtattgaaga
     4801 cgaattgaac caacgtggaa ctgttaacgt tgctgaatac cttggagaca agactttcta
     4861 caacttacca gctgttgaat tcaaaaagta gtatttcact aagaacttta gctaaataag
     4921 acccttcggg gtcttttttt ttgacacgtt tcgtattact attaaacaag taaaaggaag
     4981 tgctatatgg ttaggacaat tgtatgtgat ttagatggat ctttgatgaa tccaagtagt
     5041 ggaatctatg ttaaggaagc tgttaaagaa gcattgattg aagttgaaaa aagagatgtc
     5101 ttagtcgtat taaattcagc tcgatgcttt caaggtgtgt atccattgtc taaacagatt
     5161 ttgatgcaag attttggcgg atatgtcatt agttgtaatg gagcccattg ttttgatgtg
     5221 aaaatgaaaa agacgatatt tgagtatgta atctcaaata acgatgtttc acttatttgg
     5281 caatatgctc aaaagcataa tttaggtgtt ggatatagtc aacccgatta ttttgtggcc
     5341 aataaaatga catatgggtt tgaattggat atgcacaatt gtgatgtgga ttacatttta
     5401 acgaatcaca tggaaaaata tttaaagaat tctgtgtgga aagtgtctat ttcggattcg
     5461 aaggaaagga ttgattcgat ttatgatgaa atgaaatcaa ggattgaatc aaattgtaat
     5521 gtaagagttg tgcatagtac agatacgatg attgatatta ttcataagga ttgtgaaaag
     5581 ttgaatagtg tgaatcgttt attagaaaaa ataaacattt cttggcaaca tgttagttca
     5641 attggggatg gttctagcga tgcacctgtc ttagaagcat ctggcttagg tgtaacactt
     5701 gaaaatggtt gtgaagcctg taaaaaagtc gcaaataaaa ttgttccatc atgttatgaa
     5761 gatggctgta tagaatggtt aaaggagtta taaaaatgag agtagtcgat atgcattgtg
     5821 atacgattct agaattattg agtcgtgatt taaaagggaa agaggcaagt ttatattcga
     5881 atgatttgtc aattgattta gaaaaaatga aaaagggtaa ttatttatta cagaattttg
     5941 cactttttac agatcaaaaa gaactagcta ttcctgaaca acaaacaatg cgtttgtatg
     6001 atgaatattg tacacaaatg gataagtatg cagattatat tgcccctgta tatacgttta
     6061 gtgatattga gaagaatgat aaagaaggaa aattatcaag tttattaact ttagaagatg
     6121 gtggtgtttg ttttagtgat ttagctatgt taagaaacta ctatcgcatg ggtgtaagaa
     6181 tgattgcctt aacatggaat tatgagaacg gaattggtta tccaaacctt tctatgaaag
     6241 atatggatgg aaatcgacct aactataagc gtagtgtaga tacagaaaga ggattgacag
     6301 agtttggtat ttcatatgtg aaagaaatgg aaagacttgg tatgatcatt gatgtttctc
     6361 acttaaatga tgctggtttc tatgatgtat tgaaatatac gacaaaacca tttgtggcgt
     6421 cacattctaa cgcaagaagt gtatgtaatg tagcacgtaa tatgagtgat gatatgattc
     6481 ttcaattggc aaaacgtggt ggtgtcatgg gacttaattt ctgtggcagt ttcttggcag
     6541 atcgtgatga tcacaagagt tgtgtagacg atatggtcaa acatattcta tatatcaaag
     6601 atcttgctgg aatcgatgtg attggattag gtactgactt tgatggtatt tcatgtcctt
     6661 tagaaattga aaatgcttct atgatgaatt tattagaaga agctttatat aaggcgggtc
     6721 ttacacaaga agaagtggaa aaaatcttct ataagaatgt attaagagtc tataaatcag
     6781 tattgtaaat ggatcgtttg atccattttt ttgtttttgt tgaggaatat gatataattt
     6841 tagatgtaaa tttctgtaag aaatatgaaa gagagaatga aagattatgg aattattgaa
     6901 attattaggt attctaattg tcgtaatcgg atttgtatta aaattggatt cgattttgat
     6961 tattatgatt gccgccattg tgactgcact tgttgcaggt atggatccag ttactttctt
     7021 ggaaacactt ggatcaagtt ttgtcgctaa ccgcagtatg tgtgtgtttg tgatggtcat
     7081 ggtagttacg ggaacattag aaagaaatgg tttaaaacag gcagctgcta atttgatgat
     7141 gaaatttaaa aatgcttcag ctggtgttgt tcttgcgatt tatggtgtgt tccgtttggt
     7201 ttttgcggca tttaacgtaa gttttggtgg cgttgccgga tttgttcgtc ctattgtctt
     7261 gcctatggcg agtgctgcag ctaagaaaaa tggtaaaaag atcagtgaaa agtatgaaga
     7321 aaacttaaaa ggaatggcaa gtggtatgga aaatatcgca tggttcttcg gacaagtttt
     7381 gtttgtagga acaagtggaa tgatcttagt gcaatctaca ttagctgact taggatacaa
     7441 tgttgaattg gtcgatttaa tgaaagtgca gattccggtc gctattattg cggttggagt
     7501 tgcgattgtg tattactacc ttaaagataa acaattatct agaaaggaga ctaaataatg
     7561 aatccaattc aattttatac gagtgtggat gttacacttt cagataaact tttagaagtc
     7621 atgtattgta ttattggatt gatttctatg tatgttgcct ttaaaaatct aaaagataaa
     7681 gaaaataaaa acagtgttgg ttcatttgtt ttctggtttg atctaggagt tatgtttgtg
     7741 ctaggtaaat ggttacctgc cttggttgat ggtatcttgt tgattatctt ggttttacct
     7801 ccaattctaa agaaagtttc acctggtaat gagcctgaac ctacgcttga agagatggaa
     7861 caaaacaaca agaagattgg cgcaaaagta tttgttccag ctgtatgtat tggtttgttt
     7921 gcattacttg cagctttctt tactaagatt tcaccactag ttggaatgag tgtaggtgtt
     7981 tttgtggcca tgattatttt gcgtatctat tcaaaagcaa atacaccaag tgtcttctta
     8041 aaggattgtc gtagaatgat ggatgtggtt ggaccattaa gcatgcttcc aatgttactt
     8101 gctgctttag gtgctgtatt tactgcagcc ggtgttggag atgtgatttc tggcttagta
     8161 tcgaatatca ttcctgcagg caatgttaca attggaatta ttgtctatgc gattggtatg
     8221 gccgtgttca caatgattat gggaaatgct tttgcggcaa ttaccgtaat gacagttggg
     8281 attggtgctc cttttgtgct taaatatgga gcagatccag tagtgattgg atctttagct
     8341 ttaacttgtg gttattgtgg tacactatgt acgccaatgg cagctaactt taatatagtg
     8401 ccagtcgcaa ttctagaaat gaaagacaaa aatggtgtca ttaaaaaaca agtgcttgtt
     8461 gccgtggtta tgttagttgt acaaattgtc atgatgatca tgatgtcata ggagaaaaaa
     8521 tgttatgaaa aaagtattaa ttacaggatt tgatcctttt ggaggagaaa aaattaatcc
     8581 agcttatgaa gccgttaaat tattgccgga tgaaattagt ggggcaaaaa ttattaagaa
     8641 agaaattcct acggtatttc aaaaagggcc agaggcagtt tatgaagaaa ttcaatcttc
     8701 taaaccagat tatgtattgt gtattggtca agcaggagga agaagtcaag tcacaccaga
     8761 atgggtgggt attaacttta gaaatgcaag aattgccgat aatgaaggca atcaaccgtt
     8821 gcaaacaagt gttgtagaaa atgggccaga agcttatttt acaatgcttc ctgtgtttag
     8881 aatggttgaa aaaatgaagg aaaatggaat tcctgcaagt gttagctata cagcaggtac
     8941 ttatgtttgt aacgacgtga tgtattctgt attacattat tgtcatacag aatttaaaga
     9001 tgtaaaaggt ggctttatgc atgtaccttt tgcgacggaa caaacagtga atcagccagc
     9061 aggaacacca ggtatgaatt taaaagatat tgccaaagcc attgaattgt ctgtagaagc
     9121 tatgttagag agtgatacgg atattaaagt ggtttctgga gaaacgcatt aagagaaaag
     9181 atttattctt ttcttttttt gacttttaga atgtaagaga ttacactaaa tgtaaaaaaa
     9241 agaggtgtgt atatgaaaag aaatgaacaa ttagttgaaa atacatggaa aatagaagat
     9301 atctatccaa atctagatgt gttttataaa gaattagagg aagcaaagat gcaatcaaaa
     9361 gaattattga aatataaaga tcatctaaca aatgcatcta gtttatatgc gtatttaaaa
     9421 gggtatgaag aattaaatga aaaactatat acattggatt gttatacatc tttattaagt
     9481 gatcaagatg gttcaaatgc tttttatcaa gatttaaaag gaaaggcaaa tatagttatt
     9541 acagaaattc attctcagca agcttttttt gaagtagagc ttttaaaatt gtctgatgta
     9601 gaaacatatt ttgaaaagga acctggatta gagagatata gacgatttat tcaagatatt
     9661 tatcgattaa agccatatac tttgtctatg gaaaaagaag aattgttggc agggtgtcag
     9721 gatatttttt ctagtagtga aagagcattc tatattttga atgatgtgga tacaagattt
     9781 gaagatgtta aagatgcgaa ggggaatgtt ctaccattga catcaggaag ttatgtggat
     9841 tatttagaaa gtgcagatga gaccttaaga aagtcggctt tcgaaaatat gtatgcagct
     9901 tatggtaagg ttcaaaatac gatggcttct ttattgaatt cacaatgtaa gatgttgaaa
     9961 tatgaatcgg atttaagagg atatccaaca tcattggagg caagtacaaa taaaaatgga
    10021 gttccatcaa gtgtgtataa gaatttgatt aagcaggtac ataaggatat tgcacctttg
    10081 cataaatata tgaaggttcg taaaaaggct ttaaagaaag ataaattaaa tatgtatgat
    10141 ttatatgttc ctttagttaa ggaatttgat aagaaaatta cctttgaaac ggcaaaaaaa
    10201 gaagcgttag cttccgtggc tttattagga caagagtacg tagaggctta tgaaaaaggt
    10261 ttaaattcac gatggatcga cgtatatgaa aatgaaggaa agcgttcagg cgcctattct
    10321 tgtggtgtgc catgtcatcc ttttgtctta ttaaatcata cagatacatt aagaagtgaa
    10381 tttactttgg cacatgaaat gggtcatgcg atgcattctt atatgtcttt taaacatcag
    10441 ccttttatta atagcgatta taagattttt gtggctgaag tcgctagtac atgtaatgaa
    10501 gctttgttga tgcattattt attggaaaga actacggata aaaaagagcg tgcttatttg
    10561 attaattatc tattggaaca atatcgtgga acaatgtacc gccaggctat gcttgcagaa
    10621 tttgaaatgt tgatcaatga ggaagtggct aaaaataata tgttgagtgc tgaattattg
    10681 aataagatca atcatgattt aaatgcattt tattatggtg aagatgtgat tgtagatgcg
    10741 ccaattgatt tagagtgggc aaaaatccca cacttatata tgaatttcta tttgtatcaa
    10801 tatgcgacaa gtttttgtgc ttccatggcc atttcgcaaa agctattaaa aggcaatcca
    10861 gaagatattg agaattattt gaatttctta aagggtggat gttctaagtc tccaattaat
    10921 ttattaaaaa tggcaggcgt cgatatgaca agtccacaac caattcatga tgcgatttgc
    10981 cagtttgata agcttgtgga tgagtttgat gaattgatca attgtaaata aatgaaattt
    11041 ttctgaaaat aacttgcaaa gaaaatgaaa ggaatgtatc atatttttaa attaagaggg
    11101 tgatgtgaat gaacaataag attatgaagt caggattagc agcaatgatg gctgcaagct
    11161 tagtgggatg ttcatcttcg tcaagtgata attcgaataa tgttttgacc gttggaatta
    11221 ctgcacagat gaatggtgta ttttcaccac tgtattatga aagtgcatac gatgcatatg
    11281 cgatcaattt gatatatcaa tcaatgcttc agtatgatga aaatgaagaa ttacagccag
    11341 ttttggctaa ggagcttcct acaattagtg atgatggaaa aacggttact tttaagttga
    11401 ataaaggtgt taagttttcc gatggatcta agcttacttc tagtgatgta aaatatacat
    11461 ttacagtgtt ggcagatcct agctatacag gaagattttc gagtgatgta gaaaatattg
    11521 aaggttataa ggaatatcat gatggggatg ctacagaatt aagtggaatt gagacaccag
    11581 atgattatac ggttgttttc catatgacac agccaagaat tgatgcggta actacttttg
    11641 gtactcgtgc tatttgctcg gatacacaat ataagtacaa gaagggacac atatcaaaga
    11701 ttgaaaaaga tacttccaat ccgattggta gtggggctta taaattgaat aaatattcaa
    11761 aggcaaccgg tgctagcttt gtaagaaacg agaactttaa agctaaaaaa ggagaatatt
    11821 caattgataa ggttattatc aaagtttgca gtacttcaac tgagttaaat gaacttcaaa
    11881 aaggaaatgt ggattattta cctgagaatg cagatccaac taaaattgga caagttagtc
    11941 tagatgacaa tttgacattt aatacatacc cacgtgcgac agaaggctat gtttacttta
    12001 actgtgcaca aggtcctaca gccgatccag ccgtacgtaa agccttagct tatgcgacgg
    12061 atcgtcaagg ctttgtcgat agtttcttct cttgggaaaa agcaagtaag gaagcaaaaa
    12121 aagaaaagct agcctttgta ccagctgtat atgcgaatcc agcaagcgcc aatttaggca
    12181 cttatattcg tggtgaagag gctttggatg gtcttgaaac ttatgaattt aatgaagata
    12241 aggcaaatca aatcttagat gaagctggat gggaaatggc agatgatggt tatcgttata
    12301 aagatggtca aaaattaaga gttcgtttca tgattgcgga aggttctagt tcattggaaa
    12361 ctttgattcc tatgattgaa aagacataca aagatattgg tgttgattta aaacagacaa
    12421 ttctagaatt caattcatta ttgtcaaaaa caagtgatga tagtgcattg ggtgaatggg
    12481 aaatgagttg tcttgcgatg agctttacag gtgtagccaa tacagattta aattctatgt
    12541 tgaaaacagg ggatgtgaat aactatgcaa gactatcaga tagtgaatta gattcattat
    12601 tggatgaagc aatgtataca acagatgaag caaaatcaac agagctttat aaacagatta
    12661 tgattaagga aaatgattta ttgccatatt tagcattata tggaaaccag aatttcaact
    12721 tatataacaa gcgtgttaag aatatgaaga caggtccaat tcataactgg tcacaagcaa
    12781 tggatacagc tactttagat taagagtgta catatgtaca ttcttttttc ttgttaaaaa
    12841 aaaggggtga gtctattatt gtgactcacc aaagaaatag gaatatatga aataaacagc
    12901 tttttattag gaaagggtta tatgtattgg gttgattact gtttatttct gtatatagaa
    12961 tagttttaaa aacttaaaag aagcttaaaa aaaccgtgaa aatatttaca aatttatgaa
    13021 aaaatgtaat ttctttcttg acactatgta tatgttgagt ataataagca ctataaagta
    13081 aatcgagtaa agatagaggc agcattgcat cagagtaaaa catggagttg gcagactgtg
    13141 aagtgtttga aaggtaacca atgccgaaag aagtaaaatg gcaattttat ttcttggggt
    13201 tataaggaat acttataaca ctccttcgtc aggaagaggc gttatcttat agttaatgag
    13261 attgtttttt agctatgtgg tatgcccgca tagctttttc tattctttgc cgattagaga
    13321 aggagaaaag aaatatgaac tttaaaaaat taatggcatc tgccatggta tgtgcttttg
    13381 cactaacagg atgtggatca aacacagcta caacatcaga agataaggac acatttgtcg
    13441 taggaatgga atgtggatat gctccattta actggcaaac atctgaaaaa acagatactt
    13501 ctgtaaaatt gggtaaagct ggttatgcgg atggatatga tgtgcaagtc gctaaaaaga
    13561 ttgcgaaaaa attaggaaag aaattagtca ttaagaaaac tgcatgggat ggtttgattc
    13621 cagctttaga gaatgatgaa attgatgcga ttatcgctgg tatgactaaa gataaagatc
    13681 gtgaagaagg agctgacttt acaactccat attatgattc aaagggaatg attatgattg
    13741 ttcgtaaaga tagtgaagaa gctggatata cagatattca acaatttaca ggtaaaaaca
    13801 tcgttggtca aaaagcaaca aactatgatg atgtcattga tcaaattaaa ggtgtaaatc
    13861 acgtaacacc aaaggctact tatcctgaaa tgattttggc tttaaaacaa aatgaagtgg
    13921 atggaattac tgctgaaatg gctgttgcca aaggtgtggt agaagctaac ccagacttaa
    13981 cagtcgttca atttgcagat ggacatggat ttgattgtga tacaactgtt tcaatcgcat
    14041 taaaagaagg ttctcgtgat tcgaaattct tcaaaaaagt tcaaaaagca ttggatagta
    14101 ttagtgatga agaacgtgaa gaaatgatgg aatatgcagt tgaacaccaa ccaacagagg
    14161 attaattatg catattgata ttgcgaaaac ttggaacata tttgttcgta acatacctct
    14221 tttccagtat ggaattgaga tgactttgtt gttcgcattt gttggtacgt ttgcaggatt
    14281 tgtcgtcggc ttattagtcg gcggcattcg tgcgattgaa atcaatgaat atgattcaac
    14341 agctattaaa attttaaaga atattggaag aattattact ggcttgtata tctgggtatt
    14401 tagaggtaca ccaatgatgg ttcaagctat gtttatttat tatgcaggaa aaccagtcat
    14461 tggatggact ccgattacag caggtttaat tattatttca atcaatacag gtgcttatat
    14521 ggctgaaatt atccgttctg gaattcagtc tgtggataaa ggacaaaatg aagccgcaat
    14581 gtctttaggt atgacaacga tgcaaacttt gatgtatgtt gtatttcctc aggccattaa
    14641 aaattcattt ccttctatag gaaatcaatt gattgtaaat atcaaggata gttcaatgtt
    14701 gaatgttatt acggtaacag aattatattt ccagacaact tca
//
LOCUS       DBMDWL010000034        14296 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1435_length_14296_cov_3.919408, whole genome shotgun sequence.
ACCESSION   DBMDWL010000034 DBMDWL010000000
VERSION     DBMDWL010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14296)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_14296_cov_3.919408"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="ACZPDN_03745"
     CDS             <1..683
                     /locus_tag="ACZPDN_03745"
                     /inference="COORDINATES: protein motif:HMM:NF046806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1297767.1"
                     /translation="CTPNYLHAKHIQMALNNHKHVVCEKPMCASSKELNACFDYAKKQ
                     NCFLMEAHKTVFTPLNQKLFEIISNKQIGQVKSIDAQYATRLTETISGWHFNKPGSGC
                     MFDIGVYPICYANRMANSSILKVSRFQDEALIEYENGCVAHIATSWDVDMENTAHIYG
                     TKGSVTCKNFWKNTELVMNDKKIVVEQKSDFTGEIEHACTCIESRMIESPVMSRNASL
                     EILKVIGV"
     gene            680..1468
                     /locus_tag="ACZPDN_03750"
     CDS             680..1468
                     /locus_tag="ACZPDN_03750"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1297768.1"
                     /translation="MRKLKISRILILFVALILVVTASIYCIRTFFRQDEIIEEKSYYD
                     LDLFSMENGWMTYKDSSYKSLTGIDVSSHNQYIDWNSVKQDGIDFAMIRVGYRGAQEG
                     ILHEDAYFNTNMQAAIQNKIKVGAYFFSSAITEDEIDEEVNMVLNEIRNYKIDMPIVF
                     DMEEFEKGGRIDNLTQEQRTNLALRFCGKIKKAGYDPMIYGNMTWLYQNYDFEKISEY
                     PIWLASYSSDCPMEDKFEMWQYSNIGQVNGIEGDVDINIYLQKK"
     gene            1470..1904
                     /locus_tag="ACZPDN_03755"
     CDS             1470..1904
                     /locus_tag="ACZPDN_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297769.1"
                     /translation="MIDFEKFEKQCDEIREINAKYIEEFVDELSKKGFVDKTIKRHYE
                     NVDFYLNTYLLREDALTMENGCKDEYLSDFFGYFFIRKCMWSTPDTVKSTMASLKKFY
                     RFMYESNRLSKEDYEEFMDTIRVDKEYWIDCCSKYNDGISEW"
     gene            1910..2773
                     /gene="pepI"
                     /locus_tag="ACZPDN_03760"
     CDS             1910..2773
                     /gene="pepI"
                     /locus_tag="ACZPDN_03760"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HIR1297770.1"
                     /translation="MKIIDGYMPFKGFKTYYRIVDGGDKTPLICLHGGPGSTHNYFEV
                     LDCIAKTGRKVIMYDQIGCGKSYVEGHDELWNQETWMEELVALMEYLNIESCHLLGQS
                     WGGMLAIAYAIEKKNAKLKSLILSSTLSSASLWAKEQHRMIGFMSDDEQQAILSEDYD
                     SIAYQAANEHYMQLHCAGPFDKDTPECVTREKKAGQRSYLIGWGPNEYTPLGTLKDFE
                     YTNRLHEIDVPALIISGTNDLCTPLVAKTMYDGIKNSKWHLMPACSHMCFVDDHDTYC
                     QNLKDWLASVE"
     gene            3107..3418
                     /gene="rpsJ"
                     /locus_tag="ACZPDN_03765"
     CDS             3107..3418
                     /gene="rpsJ"
                     /locus_tag="ACZPDN_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR1297771.1"
                     /translation="MAKNTMRIRLKAYEHRILDDSAKKIVEAATKHGAKKVVGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIEIVAPNKETIDSLQRLELPSGVDIEIK
                     L"
     gene            3433..4125
                     /gene="rplC"
                     /locus_tag="ACZPDN_03770"
     CDS             3433..4125
                     /gene="rplC"
                     /locus_tag="ACZPDN_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR1297772.1"
                     /translation="MKGLLGRKLGMTQVFTEDGVLIPVTVVEVTPNVVLQKKTVETDG
                     YNAVQLGFEEIKEKRTTKANIGHCKKANTSPKRFIKEVRDCDMDVNVGDLVNVDIFAA
                     GETVDVIGTSKGKGYNGTIVRNNAHQGPKTHGGSKNIRHIGSLATNGITSRQGRIMKG
                     TIMAGQEGGYTTTNQNLTVIKVDTENNYMLIKGNVPGPRKGCVMVRTAKTSKGDKEPV
                     TLVDYTANKEVQ"
     gene            4125..4754
                     /gene="rplD"
                     /locus_tag="ACZPDN_03775"
     CDS             4125..4754
                     /gene="rplD"
                     /locus_tag="ACZPDN_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR1297773.1"
                     /translation="MATIDVINQTGAVVGSIELNDAVFGIEPHQQAMYDALVMQMASR
                     RQGTADTKGRSEVSGGGRKPYRQKGTGRARQGSIRATQFRGGGTVFGATPRNYAYRIN
                     KKVRRLAVKSALSEKVQETAFSVLDKFEIAEPKTKTFVSIIEAIQAPKKTLFVVADSE
                     EATNVYLSARNIAGATVMAASAINTYDLVNANKVVMTSAAVKQVEEALA"
     gene            4754..5047
                     /gene="rplW"
                     /locus_tag="ACZPDN_03780"
     CDS             4754..5047
                     /gene="rplW"
                     /locus_tag="ACZPDN_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR1297774.1"
                     /translation="MKDYRDVIIRPIITEATMKMMAEDNKVTFEVVKGVSKIEIAKAV
                     EAAFNVEVVKVNTMNCKPKTKRVGRYVGKTSAVHKAIVKIKEGQTIELFGSEE"
     gene            5106..5939
                     /gene="rplB"
                     /locus_tag="ACZPDN_03785"
     CDS             5106..5939
                     /gene="rplB"
                     /locus_tag="ACZPDN_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR1297775.1"
                     /translation="MPIKKYKPMTPGRRGMTTLAKDEITTSKPEKSLLAPLKSKGGRN
                     NTGRITTRHQGSGHKRTYRIIDFKRNKDNVPARVATIEYDPNRSANIALLNYADGEKR
                     YIIAPKDLKVGMTVISGTETDIKIGNCCEMRNMPEGTFIHNVELKPGKGGQMARSAGC
                     SAQILGIEEKYVTLRLSSGEVRKVLANCRATVGTVGNEDYSLVNYGKAGRNRWRGIRP
                     TVRGSVMNPNDHPHGGGEGRTPIGRKSPMTPWGKKALGVKTRRNKKASTKLIVRRRNG
                     K"
     gene            5958..6233
                     /gene="rpsS"
                     /locus_tag="ACZPDN_03790"
     CDS             5958..6233
                     /gene="rpsS"
                     /locus_tag="ACZPDN_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR1297776.1"
                     /translation="MSRSLKKGPFCDDHLMKKVVALNEAGKKEVIKTWSRRSTIFPQF
                     VEHTFAVYNGKEHVPVYVTEDMVGHKLGEFVPTRKYTGHGSDDKKSR"
     gene            6248..6583
                     /gene="rplV"
                     /locus_tag="ACZPDN_03795"
     CDS             6248..6583
                     /gene="rplV"
                     /locus_tag="ACZPDN_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR1297777.1"
                     /translation="MEVKATAKTLRIQPRKARLVLDLIRGKDVEEAAAILKFLPNKGG
                     YLVGKVLHSAVANAVNNNDLDESKLYVKACWADEGITMKRFMPRAKGSASAIHKRTSH
                     ITVVVAEKE"
     gene            6594..7406
                     /gene="rpsC"
                     /locus_tag="ACZPDN_03800"
     CDS             6594..7406
                     /gene="rpsC"
                     /locus_tag="ACZPDN_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1297778.1"
                     /translation="MGQKVSPIGMRVGVIRDWQSRWYADDKEFGTLLMEDVKIRELVE
                     AYYAKLSNEAVDKRKADPQISRIEIERTKGRMTVIIRTAHPGVVIGQDGKSIEGLKKK
                     VAKIASAKNVQINVVEVANPNLDARLVARWIANELEGRKSFRATQKKAIQNTMRAGAK
                     GIKTAVSGRLGGADMARTEGYSEGVVPLHTLRSDIDYAWEEAATQYGRLGVKVWICRG
                     EVLPGEMVKEPEAPKQRPMRKGKGRRRPDARSNRAPKDAAKTAAPAEKGGNE"
     gene            7406..7822
                     /gene="rplP"
                     /locus_tag="ACZPDN_03805"
     CDS             7406..7822
                     /gene="rplP"
                     /locus_tag="ACZPDN_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR1297779.1"
                     /translation="MLMPKRTKYRRPHRLSYEGRSKAGRTIDFGEYGLVAESGNYVSN
                     RQIEAARIAMTRYMKRGGNVWIRIFPHMARTKKPLEVRMGSGKGAPEGWVAVVQPGRV
                     MFEVGGVSEEVAREALRLAAHKLPVKTRFARKGEVK"
     gene            7822..8022
                     /gene="rpmC"
                     /locus_tag="ACZPDN_03810"
     CDS             7822..8022
                     /gene="rpmC"
                     /locus_tag="ACZPDN_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR1297780.1"
                     /translation="MLAKEIREKTNEELLQEIDTLKDELFNLRFQQATGQLENTARLK
                     TVKKTIARIKTVLTERENAGQE"
     gene            8037..8294
                     /gene="rpsQ"
                     /locus_tag="ACZPDN_03815"
     CDS             8037..8294
                     /gene="rpsQ"
                     /locus_tag="ACZPDN_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR1297781.1"
                     /translation="MERNARKVYRGTVVSDKMDKTITVMVETKKTHPLYGKRVKYSKK
                     FKAHDENNEARIGDTVVIMETRPLSATKRFRLVEIVEKARG"
     gene            8313..8681
                     /gene="rplN"
                     /locus_tag="ACZPDN_03820"
     CDS             8313..8681
                     /gene="rplN"
                     /locus_tag="ACZPDN_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR1297782.1"
                     /translation="MIQNESRMRVADNTGAKEVLVIRCLGGSVRKFANIGDVVVCAVK
                     QATPGGSVKKGDVVKAVIVRTRRGVRRENGTYIKFDDNACVIIKDDKTPKGTRIFGPV
                     ARELRDKDFMKIVSLAPEVL"
     gene            8695..9015
                     /gene="rplX"
                     /locus_tag="ACZPDN_03825"
     CDS             8695..9015
                     /gene="rplX"
                     /locus_tag="ACZPDN_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR1297783.1"
                     /translation="MNIKKGDKVKVITGAYKGTIGEVKAVFPREDRVIVEGVNMVKKH
                     LKPTQANPEGGIVEKEAKIHVSNVMLYDEKAKVASRITTKVEVVDGKKVKTRIFKKSG
                     NEVK"
     gene            9027..9566
                     /gene="rplE"
                     /locus_tag="ACZPDN_03830"
     CDS             9027..9566
                     /gene="rplE"
                     /locus_tag="ACZPDN_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR1297784.1"
                     /translation="MNRLQEKYKNVVVPQMMKDFEYKSVMEVPHVEKVVINIGVGDAI
                     TNAKLLDEAVEELTAITGQAPVVTKAKKSIANFKLREGMPIGCKVTLRKEKMYEFLDK
                     LFNISLPRVRDFRGVSDTSFDGRGNYTLGIKEQIIFPEIDFDKVDRTRGMDIVIVTSA
                     KTNEEAKALLTQMGMPFKK"
     gene            9580..9765
                     /locus_tag="ACZPDN_03835"
     CDS             9580..9765
                     /locus_tag="ACZPDN_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR1297785.1"
                     /translation="MAKKSMINKQQRPQKFKVREYTRCERCGRPHSVLRKYKLCRICF
                     RELAYKGQIPGVKKASW"
     gene            9781..10176
                     /gene="rpsH"
                     /locus_tag="ACZPDN_03840"
     CDS             9781..10176
                     /gene="rpsH"
                     /locus_tag="ACZPDN_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR1297786.1"
                     /translation="MIMTDPIADMLTRIRNALQQKHASVSMPASNEKKAIAQILKNEG
                     FITDFNIEGDVKKTLNIELKYGPNNEKVISGLRRISKPGLRVYAKVENLPSVLNGLGI
                     AIISTSNGMMTDKEARKNHLGGEVIAYVW"
     gene            10202..10747
                     /gene="rplF"
                     /locus_tag="ACZPDN_03845"
     CDS             10202..10747
                     /gene="rplF"
                     /locus_tag="ACZPDN_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR1297787.1"
                     /translation="MSRIGNKLITVPAGVTVEVAAGNEVTVKGPKGSLTRTFSSLMEI
                     HVEENVVTVKRPNDDKHTKQLHGTTRALLHNMVIGVSEGFTKDLELVGIGFRTAMKGN
                     VLTLNVGYSHQVEMPVKEGLEVTCPTATTIKVAGIDKQEVGEFAANVRAVRKPEPYKG
                     KGIRYVGENIRRKEGKTAGKK"
     gene            10769..11125
                     /gene="rplR"
                     /locus_tag="ACZPDN_03850"
     CDS             10769..11125
                     /gene="rplR"
                     /locus_tag="ACZPDN_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR1297788.1"
                     /translation="MFTKVSRNETRVRRHKRVRTKISGTPECPRLCVFRSNAHIHVQI
                     IDDVNQNTLVSASSVDMKLENGGNVEAARLVGTEIAKRALEKNIKEVVFDRGGYVYAG
                     RVQALAEAAREAGLEF"
     gene            11143..11646
                     /gene="rpsE"
                     /locus_tag="ACZPDN_03855"
     CDS             11143..11646
                     /gene="rpsE"
                     /locus_tag="ACZPDN_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR1297789.1"
                     /translation="MDRKPRRDQKEFEEQVISINRVTKVVKGGRRFRFAALVVVGDRK
                     GRVGVGTGKANEVPDAIKKAVEDAKKNIFTVPVIGTTIPHEITGRYGAGSVYMRPAIE
                     GTGVIAGGATRAILELAGISDILTKCLGSRTKINVVRATVDGLQNLRTVESVAALRGK
                     KPEEIRG"
     gene            11662..12099
                     /gene="rplO"
                     /locus_tag="ACZPDN_03860"
     CDS             11662..12099
                     /gene="rplO"
                     /locus_tag="ACZPDN_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR1297790.1"
                     /translation="MKLHTMNLAETRTERNRVGRGPGSGNGKTSGRGQKGQKSRSGGG
                     VRLGFEGGQTPLARRLPKRGFTNFNRKEYAIVNVETLNRFEDGATVNAAALIEAGVIN
                     KELSGLKVLGNGELTKKLTVEAVKFSKSAKEAIEKAGGKVEVL"
     gene            12101..13396
                     /gene="secY"
                     /locus_tag="ACZPDN_03865"
     CDS             12101..13396
                     /gene="secY"
                     /locus_tag="ACZPDN_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR1297791.1"
                     /translation="MAGKIKAMWTNKDIRNRILFTLFAFLVYRLGSAITVPDVNTADL
                     VNGIGENSLFAMLNLLGGGGLEQFSVFALGVTPYITASIIIQLLSMDVIPHLTELSKA
                     GANGRKTLDKYTRYLAVVFAFVQSFSLVYGFSKAYTGLIVGGVTISKILYIATIFAAG
                     SMFLVWVGDQISMKGIGNGISMVIMAGIVGRMPQQFSSAWSSLITGESAPTYGAWLFA
                     TYILVYLLIIIFVTLINTAERRIPIQYTSSSISLSKPSEANYLPLKVNSASVIPVIFA
                     SSLMMAPIQIASFFPSNDFIKEMQKWLGLQTWYSLVIYVLLILFFTFFYTKMQINPEK
                     VAENLGKSGSYIPSVRPGNETKEYINRVLSRITVLGSVALAIIAVMPHIMPLVWPDLP
                     NSMALGGTGMIIVVGVAIETVRQVQGLITQKSYKKYYED"
     gene            13416..14066
                     /locus_tag="ACZPDN_03870"
     CDS             13416..14066
                     /locus_tag="ACZPDN_03870"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR1297792.1"
                     /translation="MNIFIMGAPGSGKGTFSTKIKEEFNLNHISTGDIFRANIAGGTE
                     LGLQAKAYSEKGLLVPDEITNKMVKDYLNTLEDKKNGYLLDGYPRTLAQAKAFEEMTE
                     GTDLAVEKIVYMDVPFDELTRRITGRRTCKKCGEIYNIYSKPSKVEGVCDVCGGELTQ
                     RKDDNEESLKVRLDEYAKNTEPVIAYYEEKNMVSRIDASKSMEEIWPELKEALKNK"
     gene            14200..>14296
                     /gene="rpmJ"
                     /locus_tag="ACZPDN_03875"
     CDS             14200..>14296
                     /gene="rpmJ"
                     /locus_tag="ACZPDN_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR1297793.1"
                     /translation="MKVRPSVKPICDKCRVIRRKGRVMVICENPKH"
BASE COUNT         4982 a         2186 c         3107 g         4021 t
ORIGIN      
        1 tttgtacacc gaattatttg catgcgaaac atattcaaat ggctttaaat aatcataagc
       61 atgtagtgtg tgaaaaacca atgtgcgcat catcaaaaga attaaatgca tgttttgatt
      121 acgcaaagaa acaaaattgt tttttgatgg aagctcataa aacggtattt acgccattaa
      181 atcaaaaact attcgaaatc atctcaaata aacagattgg acaagttaaa tcaatagatg
      241 cacaatatgc gacaagatta acagaaacca tttcaggttg gcattttaat aagccaggat
      301 ctggatgtat gtttgatatt ggggtttatc ctatttgtta cgcaaatcgg atggcaaata
      361 gctcaatatt gaaagtgtct cgatttcaag atgaggcttt gatcgaatat gaaaacgggt
      421 gtgtggcaca tatcgcaaca agctgggatg tagatatgga aaatacagct catatctatg
      481 gtacaaaagg aagtgttact tgtaagaatt tttggaagaa cacagaactt gtaatgaatg
      541 ataaaaagat tgtggtagaa caaaaatcgg attttactgg tgaaatcgaa catgcctgta
      601 cttgtataga gagtagaatg atagaaagtc ctgttatgtc tagaaatgca agcttagaga
      661 ttttaaaggt tattggcgta tgagaaaact aaaaataagt agaattttaa ttctttttgt
      721 tgccttaatt cttgtggtga cagctagtat atattgtatt cgaacatttt ttcgacaaga
      781 tgagataata gaagaaaaaa gttattacga tttagattta ttttctatgg aaaatggttg
      841 gatgacctat aaagattctt cttataaatc tttaacagga attgatgttt caagccataa
      901 tcagtatatt gactggaatt ctgtaaaaca ggatggaatt gattttgcga tgatcagagt
      961 tggctatcgt ggtgctcaag aaggaatctt acatgaagat gcatatttta atacgaatat
     1021 gcaagcagca attcagaata aaatcaaagt tggagcgtat tttttctcga gcgctattac
     1081 ggaagatgaa attgatgaag aagtgaatat ggtattaaat gagattcgaa attataaaat
     1141 tgatatgccc attgtctttg atatggaaga atttgaaaaa ggtggaagaa tagataatct
     1201 gacacaagag cagcgaacaa atttggcact tcgattttgt ggtaaaatta aaaaggctgg
     1261 atatgatcct atgatttatg ggaatatgac ttggctttat caaaattacg attttgaaaa
     1321 gatatccgag tatccaatat ggttagcgtc ttattcttca gattgtccaa tggaggataa
     1381 gtttgaaatg tggcaatatt caaatattgg ccaagtgaat gggatagaag gagatgtaga
     1441 tataaatata tatctacaaa agaaatgata tgatagattt tgagaaattc gaaaagcaat
     1501 gcgatgaaat tcgtgaaatt aacgcaaagt atattgaaga gtttgtagat gaactttcaa
     1561 aaaagggatt tgttgataaa acgataaagc gtcactatga gaatgtagat ttctatttaa
     1621 acacatattt gttaagagaa gatgctttga caatggaaaa tggttgtaag gatgaatact
     1681 taagtgattt ctttggatac tttttcatac gtaagtgtat gtggagtaca ccggatacag
     1741 ttaagtcaac aatggctagt cttaaaaagt tttatcgatt catgtatgaa tcgaatcgat
     1801 tatcaaaaga agactatgaa gaattcatgg atacaattcg tgttgataag gaatattgga
     1861 tagattgttg cagcaaatat aatgatggaa taagtgaatg gtgatttgaa tgaaaataat
     1921 tgatggatat atgcctttta aagggtttaa aacatattat cgaattgtgg atggtggaga
     1981 taagactcca ttgatttgtt tacatggagg tccaggatca acgcataatt attttgaagt
     2041 attggattgt attgccaaga caggaagaaa agtaattatg tatgatcaaa taggatgtgg
     2101 taaatcatat gtggaaggtc atgatgaatt atggaaccag gaaacttgga tggaagagct
     2161 tgttgcgttg atggaatatt taaatattga atcctgtcat ttgcttggac agtcttgggg
     2221 tgggatgtta gcgattgcgt atgcgattga aaagaaaaat gccaaactta aatctttgat
     2281 tctttcaagt acattgtcct cagcttcatt atgggcaaaa gaacaacatc gtatgattgg
     2341 gtttatgtcg gatgatgagc agcaagctat tttaagtgag gactatgatt ctatagcgta
     2401 tcaagcggcg aatgagcatt atatgcagct tcattgcgct ggtccatttg ataaagatac
     2461 accagagtgt gtcactagag aaaagaaagc aggacaaaga tcttatttga ttggttgggg
     2521 tcctaatgaa tatacgccat taggcacttt aaaggatttt gagtatacaa atcgtttaca
     2581 tgaaattgat gtgccagcgt taataattag tggaacgaat gatttatgta cgccattagt
     2641 ggctaaaacg atgtatgatg gcattaaaaa ttcaaaatgg catttaatgc ctgcatgtag
     2701 tcatatgtgc tttgtggatg atcatgatac atattgtcaa aatttgaagg attggttagc
     2761 atctgttgaa taaagcagat gcttttttac atatttcaaa aaaacaggca tgatcttatt
     2821 gtgaaaatca acatttacga cccactgaca tcagttgtca caacataatt tttttaaaat
     2881 ccattttttt ggaaattaaa aagtgtttaa acacgataaa taaaggcttt aaaagaaata
     2941 ttaatttttt ttaatttagg ggttgaacac atgaaatata tgtgttagaa tacaggagct
     3001 tgcgatgaag tgcgaggttg ctgacccact gagaaccgtt gtcatgggtg tggccaggta
     3061 attcgcatgg agcatgttta tctagaataa acgaaaggag ttaaacatgg caaaaaatac
     3121 tatgagaatt cgcttaaagg cgtatgagca cagaatctta gatgatagtg caaagaaaat
     3181 tgtcgaagct gcgacaaaac acggagctaa aaaggttgta ggacctgttc cattacctac
     3241 tgaaaagcag gtagtaacaa ttcttcgtgc ggttcacaaa tacaaagatt cacgtgaaca
     3301 atttgaaatt cgtacgcaca agcgtttgat tgagattgtt gcaccaaaca aggaaacgat
     3361 cgattcttta caaagacttg aacttcctag cggtgtcgac atcgaaatta aactttaatg
     3421 gaggtgtcaa tgatgaaagg actattagga agaaaattag gaatgacaca agtcttcact
     3481 gaagatggtg tattaattcc agtaactgta gttgaagtaa cacctaacgt tgttttacaa
     3541 aagaaaacag ttgaaacaga tggttacaac gcagttcaat taggatttga agaaatcaaa
     3601 gaaaaacgca caactaaagc aaacatcgga cactgtaaaa aagcaaatac aagtccaaag
     3661 cgtttcatta aggaagtgcg cgattgcgac atggatgtaa atgttggcga tttagttaat
     3721 gttgatatct ttgctgcagg agaaacagta gatgtaatcg gtacaagtaa aggtaaaggt
     3781 tacaatggta caatcgttcg taacaatgct caccaaggtc caaagactca cggtggatct
     3841 aagaacattc gtcacatcgg ttcattagct acaaacggta ttacttcacg tcaaggacgt
     3901 attatgaagg gtactatcat ggctggtcaa gaaggtggat atactactac aaatcaaaac
     3961 ttgacagtta ttaaagttga tacagaaaac aactatatgt taattaaagg taacgttcct
     4021 ggacctagaa aaggttgcgt aatggtacgt actgctaaaa ctagcaaggg agataaagaa
     4081 cctgttactt tagttgacta tactgcaaat aaggaggttc aataatggct actatcgacg
     4141 taattaacca aactggtgca gttgttggtt caatcgaatt gaacgacgct gttttcggaa
     4201 ttgaaccaca ccaacaggct atgtatgatg cattggtaat gcaaatggct agccgtcgtc
     4261 aaggaactgc tgacactaaa ggtcgtagcg aagtttctgg tggtggacgc aagccttacc
     4321 gtcaaaaagg tacaggacgt gctcgtcaag gttctatccg tgctactcaa tttagaggtg
     4381 gtggaacagt atttggagct actccacgta actatgcata ccgcattaat aaaaaagttc
     4441 gccgtttagc agttaaatct gcattgagcg aaaaagttca agaaacagca ttcagcgttt
     4501 tggataaatt tgaaattgct gaaccaaaaa caaaaacatt tgtttcaatt attgaagcta
     4561 tccaagcgcc aaagaaaaca ttgtttgttg ttgctgatag cgaagaagca acaaacgttt
     4621 acttatcagc acgtaacatt gctggagcta cagtaatggc tgcttctgca atcaatactt
     4681 atgatttagt aaatgcaaac aaagttgtta tgacaagcgc tgccgtaaaa caagttgagg
     4741 aggcattggc ataatgaaag attacagaga cgtaattatc cgcccaatca tcactgaagc
     4801 aactatgaag atgatggctg aagataacaa agtaactttt gaagtagtta agggtgttag
     4861 caaaattgaa attgctaaag ccgttgaagc tgcattcaat gtagaagttg taaaagtaaa
     4921 tacaatgaac tgcaaaccaa aaacaaaacg tgtaggtcgt tatgttggaa aaacaagcgc
     4981 tgtacacaaa gctattgtaa aaattaaaga aggacaaaca atcgaattgt tcggttctga
     5041 agaataatca gggagaagac gctatttcct gaataaatat tgcgataagg aggaaaatta
     5101 agtttatgcc aattaagaag tataaaccaa tgacacctgg acgtcgtgga atgacaactt
     5161 tggctaaaga tgaaatcaca acatctaaac cagaaaaaag tttgttagca ccattgaaat
     5221 ctaaaggtgg aagaaacaac acaggacgta ttacaacacg tcatcaaggt tctggacaca
     5281 aaagaactta ccgtatcatc gatttcaaac gtaacaaaga caacgtgcct gcacgtgttg
     5341 ctacaatcga atacgatcca aaccgttcag caaacattgc attgttaaac tatgctgatg
     5401 gtgaaaaacg ttatatcatc gctccaaaag atttgaaagt tggtatgaca gttatctctg
     5461 gtacagaaac agatatcaaa attggtaact gctgtgaaat gagaaacatg cctgaaggta
     5521 catttattca caacgttgaa ttaaaacctg gcaaaggtgg acagatggct cgtagtgctg
     5581 gatgttcagc tcagatctta ggtattgaag aaaaatatgt aactttacgt ttatcttctg
     5641 gagaagttcg taaagtattg gctaactgcc gtgctactgt aggtacagtt ggtaatgaag
     5701 attacagctt agttaactat ggtaaagcag gtcgtaaccg ttggagaggt attcgcccaa
     5761 ctgttcgtgg ttctgtaatg aatcctaatg atcaccctca tggtggtggt gaaggacgta
     5821 cacctatcgg acgtaaatca ccaatgactc cttggggtaa aaaagcattg ggtgttaaaa
     5881 ctcgtcgtaa taagaaggct agtacaaaat taattgtacg tcgtcgtaat ggtaagtagg
     5941 caaggaggta agatacaatg agtcgtagtt tgaaaaaagg acctttctgt gatgaccatt
     6001 tgatgaaaaa ggttgttgca ttaaatgaag ccggaaaaaa agaagttatt aaaacttgga
     6061 gccgtcgttc tacaatcttc cctcaatttg ttgaacacac atttgccgtt tataacggta
     6121 aggaacatgt tcctgtttat gtaacagaag acatggttgg acacaaatta ggtgaattcg
     6181 ttccaacacg taaatacact ggtcatggat cagatgataa gaaatctaga tagtaggagg
     6241 agaagaaatg gaagtaaaag ctaccgcaaa aactctacgt attcaacctc gtaaagcaag
     6301 actagtctta gacttgatcc gtggaaaaga cgtagaagaa gcagctgcta ttttgaaatt
     6361 cttacctaac aaaggtggat accttgttgg taaagtatta cactcagctg ttgctaacgc
     6421 agttaacaat aacgatttag atgaaagcaa gttatatgtg aaggcttgct gggctgatga
     6481 aggaatcaca atgaaacgct tcatgcctcg tgctaaaggt agtgcgagtg ctattcacaa
     6541 acgtacaagc cacattacag ttgtagtggc tgaaaaagag taggaggtaa gctatgggac
     6601 agaaagtaag tccaattgga atgcgtgtcg gagtcattcg tgactggcaa tcaagatggt
     6661 acgctgacga taaagaattc ggaacattat taatggaaga tgttaagatt cgtgaattgg
     6721 ttgaagcata ctacgctaaa ttatcaaacg aagctgtcga caaacgtaag gcagaccctc
     6781 aaatctctcg tatcgaaatc gaacgtacaa agggtcgtat gacagttatc atccgtactg
     6841 ctcaccctgg tgtagtaatc ggtcaagatg gaaaatcaat cgaaggattg aaaaagaaag
     6901 tagctaaaat tgcttctgca aagaacgttc aaattaacgt tgtagaagtt gctaatccta
     6961 acttagatgc tcgtttagta gctcgttgga ttgcaaatga attggaagga cgtaaatctt
     7021 tccgtgcaac tcagaaaaaa gctattcaaa acactatgcg tgctggtgct aaaggtatca
     7081 aaactgcagt atctggacgt cttggtggag ctgatatggc acgtactgaa ggatattcag
     7141 aaggagttgt tcctttacat acacttcgta gtgatattga ctatgcttgg gaagaagcag
     7201 cgactcaata tggtcgttta ggtgtaaaag tttggatctg ccgtggtgaa gtattacctg
     7261 gtgaaatggt taaagaacca gaagctccaa aacaacgccc aatgcgtaaa ggaaaaggac
     7321 gtcgtcgtcc agatgctcgt tcaaaccgtg cccctaaaga tgctgctaaa actgcagcac
     7381 ctgcagaaaa aggaggtaac gaataatgtt aatgcctaaa cgtacaaagt atcgtcgtcc
     7441 tcaccgtttg agctacgaag gtcgttcaaa agcaggtcgt acaattgact ttggtgaata
     7501 tggattggtt gcagaatcag gtaactatgt aagcaaccgt caaattgaag cagcccgtat
     7561 tgctatgaca cgttatatga aacgtggagg aaatgtttgg attcgtattt tccctcacat
     7621 ggcacgtact aaaaaacctc tagaggttcg tatgggttct ggtaaaggtg ctcctgaagg
     7681 atgggtagcc gtagttcaac caggacgtgt aatgttcgaa gttggtggag taagtgaaga
     7741 agtagctcgt gaagctttac gtcttgctgc tcacaaatta ccagttaaaa ctcgttttgc
     7801 tagaaaagga gaagtgaagt aatgttagct aaagaaatcc gcgaaaaaac aaatgaagag
     7861 cttcttcagg aaatcgatac gcttaaagat gaacttttta accttcgttt ccagcaagcc
     7921 acaggacagt tagaaaatac agctcgttta aaaactgtta agaagacaat tgctcgtatt
     7981 aaaactgtat tgacagagcg tgaaaacgct ggtcaagagt agggaggacg tatctaatgg
     8041 aaagaaacgc tcgtaaagta taccgtggta cagttgtgtc tgataaaatg gacaaaacta
     8101 tcacagttat ggtagaaact aaaaaaacac acccattata tggtaaacgt gttaaatatt
     8161 ctaagaaatt taaagctcac gatgaaaaca acgaagctcg tatcggagat acagtagtaa
     8221 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttagtagaa atcgttgaaa
     8281 aggctcgtgg ttaatactag gaggagaaag caatgattca aaacgaaagt cgtatgcgcg
     8341 ttgctgacaa cactggagct aaagaagttt tagttatccg ttgtttaggt ggtagcgtta
     8401 gaaaatttgc aaacatcggt gatgtcgttg tatgtgctgt taaacaggct actcctggtg
     8461 gatccgtaaa aaaaggtgat gtagttaaag ccgtaatcgt acgtacacgt cgtggtgttc
     8521 gtcgtgaaaa cggaacttac atcaagttcg atgataacgc ttgtgtaatc atcaaagatg
     8581 ataagacgcc aaagggaact cgtatcttcg gtccagttgc acgtgaatta cgtgacaaag
     8641 acttcatgaa gatcgtttct cttgcgccag aagtattata ggaggtgtgt ctaagtgaac
     8701 attaaaaaag gtgataaagt aaaagttatt acaggtgctt acaaaggcac aatcggtgaa
     8761 gtaaaagctg tattccctag ggaagataga gttatcgttg aaggtgttaa catggttaaa
     8821 aaacacttga agccaactca agctaatcct gaaggtggaa tcgttgaaaa agaagctaaa
     8881 attcacgttt caaatgtaat gctttacgat gaaaaagcaa aagttgcaag ccgtattaca
     8941 acaaaagttg aagttgtaga tggtaaaaaa gttaaaacac gtatctttaa aaagagcggt
     9001 aacgaagtga agtaggaggg tagaagatga atcgtttaca agaaaaatat aaaaacgtag
     9061 ttgtccctca aatgatgaaa gatttcgaat acaaatctgt catggaagta cctcacgttg
     9121 aaaaagtagt aatcaacatt ggtgtagggg atgccatcac taatgcaaaa ttattagatg
     9181 aagctgtaga agaattgaca gctatcactg gtcaagctcc agttgtaaca aaagctaaaa
     9241 aatcaattgc gaacttcaaa ttgcgtgaag gtatgccaat tggatgtaaa gttactttac
     9301 gtaaagaaaa aatgtatgaa ttcttagata agttattcaa catttcatta cctcgtgtac
     9361 gtgacttccg tggagttagc gatactagct ttgatggtcg tggaaactat acattaggta
     9421 ttaaagaaca aatcattttc cctgaaattg atttcgataa agtagaccgt actcgtggta
     9481 tggacatcgt aatcgttaca tcagcgaaaa caaacgaaga agcaaaggca ttattaactc
     9541 aaatgggtat gcctttcaaa aagtaggagg agcttttaaa tggcaaagaa atcaatgatt
     9601 aacaaacaac agcgtcctca aaaatttaaa gttcgtgagt acactcgttg tgaacgttgt
     9661 ggtcgtccac actctgtatt aagaaaatac aaattatgtc gtatttgctt ccgtgaattg
     9721 gcttacaaag gtcaaattcc tggagttaaa aaggctagtt ggtaaggagg tatattgcat
     9781 atgattatga ctgatcctat tgcggatatg ctgactagaa tccgcaatgc tttgcaacaa
     9841 aagcatgcct ctgtcagtat gccagcgagc aatgaaaaga aagcaattgc tcaaattttg
     9901 aaaaatgaag gtttcataac tgacttcaac attgaaggtg atgttaagaa aactttgaac
     9961 attgaattga aatacggacc taacaatgaa aaagtaatca gcggattacg tcgtatttct
    10021 aaacctggtc ttcgtgttta cgctaaagta gaaaacttac ctagcgtatt gaacggatta
    10081 ggaatcgcaa tcatttcaac aagcaatgga atgatgactg acaaagaagc aagaaaaaac
    10141 cacttaggtg gagaagttat tgcttacgtt tggtaataaa tatataggag gataaaacgt
    10201 aatgtcacgt atcgggaata aactgattac cgttcctgca ggcgttactg ttgaagtagc
    10261 tgctgggaat gaggtgactg ttaaaggacc taagggttct ttaacacgta cattctcttc
    10321 tttgatggaa atccacgtag aagaaaatgt tgtcactgta aaacgtccaa atgacgataa
    10381 acacacaaaa caattacacg gaacaactcg tgcattgtta cacaacatgg taattggtgt
    10441 atctgaagga ttcactaaag acttagaatt agttggtatc ggattccgta ctgcaatgaa
    10501 aggaaacgta ttaactttaa acgttggata ttctcaccaa gttgagatgc cagttaaaga
    10561 aggcttagaa gtaacttgtc caactgctac aactatcaaa gttgcaggta tcgataaaca
    10621 agaagttgga gaatttgcag caaatgttcg tgctgttcgt aaaccagaac cttacaaagg
    10681 aaaaggtatt cgctatgttg gtgaaaacat ccgtcgtaag gaaggtaaaa cagcaggtaa
    10741 gaaatagtag tgggaaagga gatcaaaaat gtttactaaa gtatcacgta atgaaactcg
    10801 tgtacgtcgt cacaaacgtg tacgtacaaa aattagcgga actcctgaat gcccacgttt
    10861 atgcgtattc agaagtaacg cacacatcca tgtacaaatc attgatgatg ttaaccaaaa
    10921 cacattggtc agtgcttcaa gtgttgacat gaaattagaa aatggtggaa acgtggaagc
    10981 agctcgtttg gtaggaactg aaattgctaa gcgtgcacta gaaaagaata ttaaagaggt
    11041 tgtatttgac cgtggtggat atgtttacgc tggtcgtgta caagctttag cagaagcagc
    11101 tcgtgaagct ggcttggaat tttaggaggt cgcacaatgg caatggatag aaaaccaaga
    11161 agagaccaaa aagaatttga agaacaagta atctcaatca accgtgtaac taaagtagtt
    11221 aaaggtggac gtcgtttccg ttttgctgca ttagttgtag ttggagatcg taaaggccgt
    11281 gtaggtgttg gtacaggaaa agctaatgaa gttcctgatg caatcaaaaa agcggttgaa
    11341 gatgctaaga aaaacatctt cacagtacca gttatcggta caacaatccc tcacgaaatc
    11401 actggtagat atggtgcagg atctgtatac atgcgtccag ctatcgaagg tactggtgtt
    11461 atcgctggtg gtgcaactcg tgcaatcctt gaacttgcag gtatctctga tatcttaact
    11521 aaatgtttag gatcacgtac aaaaatcaac gttgttcgtg caacagttga tggattacag
    11581 aacttaagaa ctgtagaaag cgttgctgcg cttcgtggta agaagccaga agaaattcgc
    11641 gggtaatagg aggtaaatca gatgaaatta catacaatga atttagccga aactcgcaca
    11701 gaacgtaacc gtgttggtcg tggacctgga agcggaaacg gaaaaacaag cggtcgtgga
    11761 caaaaaggtc aaaaatcaag atcaggtgga ggagttcgct taggttttga aggtggtcaa
    11821 acaccattag caagacgttt accaaaacgt ggattcacaa acttcaaccg taaagagtat
    11881 gctatcgtta acgttgaaac attaaatcgt ttcgaagatg gagcaacagt taatgcagct
    11941 gctttaattg aagctggtgt tatcaataaa gaactttctg gacttaaagt attaggtaac
    12001 ggtgagttaa ctaaaaaatt aactgttgaa gcagttaaat tctctaagtc agctaaagaa
    12061 gctattgaaa aagcaggcgg aaaagtagag gttctataat atggcaggca agataaaggc
    12121 aatgtggacg aataaggata tccgcaatag gattctattc accctatttg cctttcttgt
    12181 ctatcgtttg ggatcagcca ttacagttcc tgatgtaaat acagcagatc ttgtaaatgg
    12241 tattggagaa aactcattgt tcgcaatgtt aaatctattg ggaggtggag gacttgaaca
    12301 gttctctgtc ttcgctttag gtgtgactcc atatattact gcttctatca ttatccaatt
    12361 attaagtatg gatgtaattc cacacctaac tgaattgagt aaagctggag caaatggtcg
    12421 taaaacatta gacaagtaca ctagatatct tgcggtcgtt tttgcctttg tacaatcatt
    12481 ttcattagta tacggttttt caaaagcata tacaggattg attgtcggcg gtgttacaat
    12541 cagtaagatt ttatatattg caacaatttt tgctgcagga agtatgtttt tagtgtgggt
    12601 tggagatcaa atttcaatga aaggtattgg aaatggtatt tcaatggtaa ttatggcagg
    12661 tattgtaggg cgtatgcctc aacaattttc tagtgcctgg tcaagcttaa ttacgggtga
    12721 gtctgcgcca acctatggag cttggttatt tgccacatat atattagttt atttattaat
    12781 tattatattt gttacactaa ttaatacagc tgaaagaaga attccaattc aatatacatc
    12841 aagttcaatt tcattaagta aaccaagtga agcaaactac ttgccattga aagtcaattc
    12901 agcaagtgtg attccagtca tctttgcaag ttcattgatg atggcaccaa ttcaaattgc
    12961 ttcattcttc ccttcaaacg atttcattaa agaaatgcaa aaatggttag gattacaaac
    13021 ttggtattca cttgtaatct atgtattatt gattttattc tttacattct tctatacaaa
    13081 gatgcaaatc aatccagaaa aagttgctga aaatttaggt aaatcaggtt catatattcc
    13141 tagtgttcgt ccaggtaatg aaacaaaaga atatatcaat cgcgtattat caagaattac
    13201 agtgcttgga tctgtagctt tagcaattat tgctgttatg ccacatatca tgcctcttgt
    13261 atggcctgat cttccaaatt caatggcttt aggtggaaca ggaatgatta tcgttgttgg
    13321 tgttgctatt gaaactgtac gccaagttca aggattgatt actcaaaaat cttacaaaaa
    13381 atattacgaa gactaatatc ttcaggagaa tagatatgaa tatatttatt atgggtgctc
    13441 ctggatctgg taaaggtaca ttctctacaa aaattaaaga agaatttaac ttaaaccaca
    13501 tttcaacagg agatatcttc cgtgcaaata ttgctggagg aacagaatta ggtctacaag
    13561 caaaagctta ttctgaaaaa ggattgttag ttcctgatga aatcacaaac aaaatggtta
    13621 aggattactt aaacactttg gaagataaaa agaatggata tttattagat ggatatccaa
    13681 gaactttagc tcaggcaaaa gcttttgaag aaatgactga aggaacagat ttagcagttg
    13741 aaaaaattgt ttacatggat gttccatttg acgaattaac tcgtcgtatt acaggtcgtc
    13801 gtacttgtaa aaaatgtgga gaaatctaca acatctattc aaaaccatca aaagttgaag
    13861 gtgtttgtga tgtttgcggt ggagaattga ctcaacgtaa agatgacaat gaagaaagct
    13921 taaaggttcg tttagatgaa tatgcaaaga atacagaacc tgtaattgca tattatgaag
    13981 aaaaaaatat ggtttcaaga atcgatgcgt caaaatctat ggaagaaatt tggccagaat
    14041 taaaagaagc cttgaaaaac aagtaagttg cgcttataat atataagcgc aactttgctt
    14101 gcgattatac attcacgttt ctgttaaaat ccgcatgtac acatacgcat tttagcaggg
    14161 ccattaaaat acgtgaatca ttacgtagga ggaagaaaga tgaaagtaag accatctgtt
    14221 aaaccaatct gcgacaagtg tcgagtaatt cgacgcaaag gacgtgtgat ggtaatttgt
    14281 gaaaacccaa aacaca
//
LOCUS       DBMDWL010000035        14153 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1452_length_14153_cov_2.883575, whole genome shotgun sequence.
ACCESSION   DBMDWL010000035 DBMDWL010000000
VERSION     DBMDWL010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14153)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_14153_cov_2.883575"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="ACZPDN_03880"
     CDS             <1..570
                     /locus_tag="ACZPDN_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB
                     C-terminal domain-containing protein"
                     /protein_id="HIR1297794.1"
                     /translation="IQNDKGDSFFPVFSSMDQLKKWDQDSEIHSLVMTYNQYMPFVKM
                     ALNQIHGIVIDPFGANVLLDCNLLVELDKSRGSQVSENPIHKGDSLKLRDPISSVTEL
                     QRAICEFGDSHPDILSIYLKERMVKDQPSHWFLVVDMINDDKQTIQDLGNFCTPVSYG
                     KGFEFMFGSMELAKKIMADTIPTYVKVTK"
     gene            612..1916
                     /locus_tag="ACZPDN_03885"
     CDS             612..1916
                     /locus_tag="ACZPDN_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR1297795.1"
                     /translation="MKLPEYILEIIQKINDIGYEAYVVGGCVRDYLLDRPIHDYDICT
                     SAKPEVILNLFDRSIGTGLKHGTVTVLSNSPVEITTFRLESEYKDHRHPDSVKYVSTI
                     EEDLSRRDFTVNAMAYHPSRGLIDPYGGQFDLKAKVIRCVGNPDRRFNEDALRMLRAY
                     RFCAKLGFSMDETVKKSIDTNASLIQYVSIERIVHELKEIMETNPQVLADMTLLLKPV
                     FNELDLALRCSQNSIYHYADVLHHTLDAMCYLKPYDETLAFALLFHDLGKVQVKTTDS
                     NGKDHFKHHAKVGSELSKELCRRFKLTNEQRKWIPFYVLHHDDKFTCDLDCIFKYRVT
                     YHLDEEHLLNLLQIRYCDAMAHSELGQKSAKDVELFYVYYIQNRNRCMSISQLALNGK
                     DIIEATNLRGKQIQEALNMCLKYAFYHPEKNRKEELLNIIKK"
     gene            complement(1918..3297)
                     /locus_tag="ACZPDN_03890"
     CDS             complement(1918..3297)
                     /locus_tag="ACZPDN_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297796.1"
                     /translation="MKFTTASEVDLGHEKVGKLLFMLAVPAILSQIVNLLYNVVDRMY
                     IGHIQNVGATALTGVGVCLPIIMIVSAFACLVGMGGAPRASIYMGKQDNPTAEKIMGN
                     CFIALIVISLTLTVCLLAFQTPLLQLFQASSKTIVYAKQYMGIYALGTIFVQMTLGMN
                     AFISCQGFSKTSMMTVLIGAISNIILDPIFIFGFNMGVSGAALATIISQAISAIWVIR
                     FLSGKQTVLKLKKENFKIIPSLLFPSLALGIAPFVMQSTESLISLCFNMQLSKFGGDV
                     AIGSMTILTSVMQFAMMPIQGLTQGGQPIISYNFGARNGQRVKKGFLLQTLCCFTYAS
                     IIWILVELFPQVFISIFTNDPDLMAMASWAIRIYMACVLLMGLQISCQQTFIAFGNAK
                     ISVFLAIFRKIIVLIPLIFILPNILSDHIFAVFVAEPIADFIAVSTTVTLFIKNFKKT
                     LAQMSYEKS"
     gene            complement(3299..3730)
                     /locus_tag="ACZPDN_03895"
     CDS             complement(3299..3730)
                     /locus_tag="ACZPDN_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1297797.1"
                     /translation="MLTIEEFLRLSNRLREYYGKQIKDRFSEYTFSPNEISILILLQN
                     NTSITTSTQLRVVLGVSKALVSRSVTSLQQKGLITMKKVSENRRISYIELTEEAIPVL
                     EKISIEIDKINQVLFKDIPTKDIQCMIDTMNKMNEKIEGAN"
     gene            3987..4829
                     /gene="licT"
                     /locus_tag="ACZPDN_03900"
     CDS             3987..4829
                     /gene="licT"
                     /locus_tag="ACZPDN_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HIR1297798.1"
                     /translation="MRLKKAFNNNALLALDENEEEVIVMGKGIAFGKKSGDRIDESLI
                     QKKFVFDKSELNEKFSQLFDEIPQQYVELTVSIVEMAQKELGVRFDSSIYLALSDHIT
                     YAVRRYKKNENLKNALLFEVKKFYPNEFKAALKSLDMIQYETGCIMTEDEAGYIAMHF
                     VNAQQNGEEMSQTVKVTKMVEDILHIVEYHYQIKLDENSLNYTRFVTHIRYFARRLFA
                     KELNQDTDDILYQQIRERYPDAYRCVLKVKKYIKENCDIELTNEEMTYFMLHINRVCS
                     RIKS"
     gene            5017..6873
                     /locus_tag="ACZPDN_03905"
     CDS             5017..6873
                     /locus_tag="ACZPDN_03905"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIR1297799.1"
                     /translation="MNKYETIANEVVKNIGGTENIESVTNCMTRLRFTLKNVSKVDVE
                     SLEKVKGVQGVVNKNGQFQVVIGSDVSHVCDEIKKMGSFSETVNANSEENVSLVNRVL
                     GAITAIFQPVIPAICGAGMIKAVLALLTAFHVVEATSQTYVLLAMVADSAFYFLPIFL
                     AVSSAKRFGCNTFIAAVLGAMLIHPTYAGIVSAGEAFKFIGLPIKLVSYGSSVVPAIL
                     IVWAQSYVEKFAKKISPNAVKVFLVPLIVFLIMTPLAFVVIGPLGSFVGDILCNIFDF
                     LNNQARWVIPVLMGAFCPLFVMTGMHYSFMPVQLAQYATLGYGTLLGPGMLASNISQA
                     GACFAVALRTKDKEMKSTALSSATTALFGITEPALYGVTMKLKKPLWTVMISGGIAGL
                     WGGLTNMRTYASATAGVTALPVYICDDLSNVRNAFICIVIALVSSFVLTLLFGVPKNE
                     DTSSDNEVFEVDNTKLNKYTEIVSCMNGKVVPLENVEDDVFSKKIMGPGVAILPSEGK
                     VYAPFDGVVNTVFPTKHAYGLTSVDGIETIIHLGLDTVNLEGKYFDAKVKSGQSVKKG
                     DLLAEFDLEALQNEGYNMVTPVIITNSDQFIDVIETQKSDVKVGDELVKVFR"
     gene            6897..8306
                     /locus_tag="ACZPDN_03910"
     CDS             6897..8306
                     /locus_tag="ACZPDN_03910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR1297800.1"
                     /translation="MKFPKEFLWGGATAANQCEGAWNEDGKGASIQDYMPHGIMQGPS
                     EVIDDHNLKLKGIDLYHHYKEDIALFAEMGFKVYRLSIAWSRIFPNGDDEKPNEKGLE
                     FYDNVFDECKKYGIEPLVTLSHYETPYYLAKKYDGWKSRDLIGFYEKYVRTVFTRYKG
                     KVHYWITFNEINSIWHFPLMGAGILTPKNLLNAQDLYQAAHHELVASALATKIGHEID
                     SENKIGCMVLGLTSYPRTCNPDDVIATMEESKRGYFFTDIHMRGYYPSYALKMMEKEG
                     VVLDATDEDLEILKNTCDFLSFSYYMSKCIASNPEQYEKGKGNLTTGVKNPYLQESQW
                     GWQIDPKGLRYLLNTYYDRYQKPLFIVENGLGAKDTLLSEEKDGYWVEDDYRIQYMND
                     HLTQVSKAINDDGVEVMGYTSWGCIDLISASTAEMKKRYGFIYVDRNNDGTGSFKRYK
                     KKSFYWYKKVIETNGQIIS"
     gene            8423..10759
                     /gene="feoB"
                     /locus_tag="ACZPDN_03915"
     CDS             8423..10759
                     /gene="feoB"
                     /locus_tag="ACZPDN_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1297801.1"
                     /translation="MTLKELKIGQSALIKAVGGTGALRQHFLDMGVIPGAEVTVVKFA
                     PMGDPMELQIHGYELSLRLDEAEKILVELIDERTRKHESAENINNTVHPGLGEDGKYH
                     VKADEHPLPEGTCLTYALVGNQNCGKTTLFNQLTGSNQHVGNFPGVTVDRKDGPIKGY
                     PNTMVTDLPGIYSMSPYTSEEIVSRNFVLNDKPKAIINIVDATNIERNLYLTMQLLEM
                     NIPMVVALNMMDEVANNQGSIDINGMEAMLGVPVIPISAAKNQGVDELIEHAIHIAKY
                     QERPGRLDFCGEDDFGGAVHRCIHSICHLIEDHAKKTDVPLRFAASKIIEGDNLILDR
                     LDLDENEKEMIEHIVLQMEKERGLDHSAAIADMRFSFIEKVCEQTVVKPKESKERVRS
                     EKIDRVLTGKYTAIPMFIGIMLLVFYLTFNVVGAWLQGLLEFGIDWITQVVDAWMTSA
                     HVSYAVHSLVIDGIFAGVGSVLSFLPIIVTLFFFLSMMEDSGYIARVAFFMDKLLRKI
                     GLSGRSIVPLLIGFGCTVPAVMSTRTLPSERDRKMTILLTPFMSCTAKLPIYAFFVNA
                     FFRKQGGLVMTGLYVLGIAVGILIAFLFKSTLFKGEAVPFVMELPNYRLPGLKNVSQL
                     LWEKAKDFLQKAFTVIFVATVLVWFLQSFNLQFDMVKDSSQSILAMIAGWIAPVFAPL
                     GLQDWRIVTSLISGFMAKESVVSTLQVLFVGGVHSALTTLSAASLLVFCLIYSPCVAA
                     IASIKRELGARWAIGVVAWQCVLAWILAFIVHVIGSVL"
     gene            10835..11653
                     /locus_tag="ACZPDN_03920"
     CDS             10835..11653
                     /locus_tag="ACZPDN_03920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1297802.1"
                     /translation="MYKLIACDLDETLIKPDRTIDSRDIEAIKKLKDLGVKFVLATGR
                     GYNTVQGTLKELDLFDETNQYVISYNGGAITENKDNRLLHFEGISFEKAEELYKRGLN
                     YDVVIHVYTRDAVYAYNLTEDEKRYVQNRQEMIEVFDQNLDFLKGQEIVKVLYTNTDF
                     EYLQSIDRDLKDITYDMDVSYSSNRYIEFNHKGVNKGAGLKKLADLLHIDIQDTIAIG
                     DNYNDLSMIKDAGLGVGVQNAAPGIKPDCDYITTATCEEGAVCEVIEKFVLKGE"
     gene            11655..12203
                     /locus_tag="ACZPDN_03925"
     CDS             11655..12203
                     /locus_tag="ACZPDN_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1297803.1"
                     /translation="MKKLIQLYVSWFKMGLFTFGGGYAMLPMIQKEVIEKYHWATEDE
                     VMDYYAIGQCTPGVIAVNTSTFIGYYQAGVPGAIAATLGVVTPSIIIISIIAGLITNF
                     SSLAIVQHALNGIRVAVCMLMINAVLKLGKGNIKNKIGFLIFAVALIFAMFTKVSTVL
                     LVIGAGIAGVVFSRIGWLKKND"
     gene            12196..12744
                     /locus_tag="ACZPDN_03930"
     CDS             12196..12744
                     /locus_tag="ACZPDN_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1297804.1"
                     /translation="MTKLLLMFLEFFKTGLFAVGGGLATIPFLREIGVRYGWYTMSDL
                     STMIAVSESTPGPMGVNMASYVGFSQYGVLGSIVVTLGLVLPSLIIICIIANFLEKFK
                     EAKLVQQIFAGLKPAVVAFIAVATIDILVTTLFGDYTFSTFHWKQFICLFILLALSKY
                     KPKLHPIWFIVASAVVGIVFAF"
     gene            13036..13986
                     /gene="bsh"
                     /locus_tag="ACZPDN_03935"
     CDS             13036..13986
                     /gene="bsh"
                     /locus_tag="ACZPDN_03935"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIR1297805.1"
                     /translation="MCTALSFNQFFGRNLDYEFSYGEQVAVTPRNYDFHFRHLDQHES
                     HYALIGMAHVFEEYPLYYDAMNEKGLGMAGLNFVGNAKFYEVKEDKNNVAAFEFIPWI
                     LSQCASVKEARVVLENTNVCATPFNEHFPVAELHYMISDENESIIVECMEDGMHVYDN
                     PTHVLTNNPPFPMQLFRLNDYMYLSNKQPKNNFGLDLTAYSRGFGAMGLPGDLSSGSR
                     FVRVAYTRANATSDKNDLNQYFHIIGSVTQQKGLCEVEPGKYEYTIYTSCCDLRNGIY
                     YYTTYNNHQISGVNMFNENLDSEVLISYPIFEEEMIHMQN"
BASE COUNT         4631 a         1897 c         2882 g         4743 t
ORIGIN      
        1 attcaaaatg ataagggaga tagtttcttt cccgtcttct caagtatgga tcaattgaaa
       61 aaatgggatc aagatagtga aatccattcc ttggttatga catataatca atatatgcca
      121 tttgtgaaga tggcattaaa ccagattcat ggcattgtga tcgatccatt tggagcgaat
      181 gttttattag actgcaactt attggttgaa cttgataaaa gtcgtggttc acaagtgagt
      241 gaaaatccaa ttcataaagg agatagttta aagcttcgtg atcctatttc aagtgtaaca
      301 gaacttcaaa gagctatctg cgaatttggt gactctcatc cagacattct ttctatttat
      361 ttgaaagaaa gaatggtcaa agatcagcct agccattggt tcttagttgt ggatatgatt
      421 aatgatgata aacaaacaat tcaggattta ggaaacttct gtacgcctgt aagttatgga
      481 aaaggttttg aatttatgtt tggaagtatg gaacttgcca aaaaaatcat ggcggatacc
      541 attccaacat atgtaaaggt tacaaagtaa ttaaggtgca attcagcacc tttttcttat
      601 ataatagaag tatgaaatta cctgaatata tattagaaat cattcaaaaa ataaatgata
      661 ttggatatga agcgtatgtg gtaggtggat gtgtacgtga ttatttacta gatcgtccaa
      721 ttcatgatta tgatatttgt acaagtgcca aacccgaagt gatattaaat ctatttgatc
      781 gaagtatcgg aacaggactc aagcatggaa cggtaacggt tttatcgaat tcacccgttg
      841 aaatcacaac ttttcgttta gagagtgaat acaaggatca tcgtcatcca gacagtgtga
      901 aatatgtttc tacaattgaa gaagatttat caagacgtga ttttactgtg aatgcgatgg
      961 cttatcatcc aagtcgtggc ttgattgatc catatggagg acaatttgat cttaaagcga
     1021 aagtcattcg atgtgtgggt aatccagatc gaagatttaa tgaagatgct ctaagaatgt
     1081 tgcgtgctta tcgtttttgt gcaaaacttg gcttttctat ggatgaaaca gtaaaaaaat
     1141 ccattgatac aaatgcatct ttgattcaat atgtatcgat agaaagaatt gtgcatgaat
     1201 taaaagaaat catggaaaca aatccacaag tattggccga tatgacactt cttttaaaac
     1261 ccgtatttaa tgagttggat ttggcattga ggtgtagtca aaactcaatc tatcactatg
     1321 cggatgtttt gcatcatact ttagatgcga tgtgctattt aaaaccatat gatgaaactt
     1381 tggcctttgc gctattattc cacgatttag gcaaagtaca agtaaagaca accgattcaa
     1441 atggaaagga tcattttaaa catcatgcga aggtaggaag tgaattatcg aaagagcttt
     1501 gtcgacgttt taaactcaca aacgaacaaa gaaagtggat tccattctat gtcttgcatc
     1561 atgatgataa attcacttgt gatttagact gtatttttaa gtatcgcgtt acatatcatt
     1621 tggatgaaga acacttattg aacttacttc aaatccgcta ttgtgatgcg atggcccatt
     1681 ctgaattagg gcaaaaatca gctaaagatg tagaactttt ctatgtgtat tacatccaaa
     1741 atagaaatcg ctgtatgagt atatctcaac ttgcattgaa tggaaaagat ataatagaag
     1801 ctacaaattt aaggggaaaa cagattcaag aagctttgaa tatgtgttta aaatacgctt
     1861 tttatcatcc ggaaaagaat aggaaagaag aattattgaa tattataaaa aaatgagtta
     1921 tgatttttcg taactcattt gtgctaatgt ctttttaaaa tttttaataa ataacgttac
     1981 tgttgtgctt accgcaataa agtctgcgat cggttctgca acaaataccg caaagatatg
     2041 atcggataag atgtttggta agataaaaat caatggaatt aagacgataa tctttctaaa
     2101 gatagctaaa aatactgaaa tcttggcgtt tccaaacgca atgaaggttt gttggcaaga
     2161 aatctgaagt cccatcaata atacgcatgc catatagata cgaatggccc agcttgccat
     2221 tgccattaag tctggatcgt ttgtgaaaat agaaataaat acttgaggga ataattccac
     2281 tagaatccag atgatacttg cgtatgtgaa acaacatagt gtttgaagta aaaagccttt
     2341 tttaacgcgc tggccatttc tggcaccaaa gttataactg atgatgggtt gtccaccttg
     2401 cgtcaagcct tgaattggca tcatcgcaaa ctgcataaca ctcgttagaa tggtcatact
     2461 tccaatcgca acgtccccac caaatttaga gagttgcata ttgaaacata aagaaatcaa
     2521 gctttctgtt gactgcatca caaaaggtgc gatacctaaa gctaaactag gaaataataa
     2581 gcttggtatg attttaaagt tttctttttt taacttaaga accgtctgtt taccactcaa
     2641 aaaacgaatg acccagattg cactgatagc ttgcgaaatg atggtagcta gtgcagcacc
     2701 acttactccc atattgaatc caaagataaa gatggggtct aaaataatgt ttgagatagc
     2761 tccaattaaa acggtcatca tacttgtttt agagaatccc tgacaagaaa taaaggcatt
     2821 catcccaagg gtcatttgaa caaaaatggt tcctaaggca taaataccca tatattgttt
     2881 ggcataaaca attgtttttg aacttgcctg gaataattgt agtagtggtg tttgaaaagc
     2941 taataaacat acagttagag ttaaagaaat cacaatcaat gcgataaaac aatttcccat
     3001 gattttttca gccgttggat tatcttgttt acccatatat atactagctc ttggagcacc
     3061 tcccataccg actaaacacg caaaggcgga tacaatcatt ataattggaa gacaaacgcc
     3121 tacgcctgtt agagcagtag ctcctacatt ttgaatgtga ccaatataca tgcggtcaac
     3181 tacattatag agcaagttaa caatttgact taagatggcg ggaaccgcca acataaagag
     3241 taacttacca actttttcgt gtcctaaatc cacttcggac gctgttgtaa atttcatttt
     3301 agtttgctcc ttctattttt tcattcattt tattcattgt atcaatcatg cattgaatat
     3361 cttttgtggg aatatccttg aataaaactt gattgatttt atcaatctca atagatattt
     3421 tttctaatac aggaatcgct tcttccgtta attcaatata agaaattctt ctattttctg
     3481 aaactttctt cattgtgatc aatccctttt gttgcaaact cgttacgctt cgggaaacca
     3541 aagcttttga aacaccgagt acaacacgaa gctgtgtact tgtcgtaatg gatgtgttgt
     3601 tttgtagtaa aattagaatc gaaatttcat ttggggaaaa agtgtattct gaaaatcgat
     3661 ctttgatttg tttgccataa tattcacgca agcgattgga taaacgtaaa aattcttcta
     3721 tagtcaacat aggccctcct aacgtttact tgtgaacagt tcaattgtaa acaagtgaat
     3781 gaatgatgtc aatgatttgt atttaaagta attatatttt ttattgacat aacaaaaaca
     3841 tcgtgttacc atgtaaacgc attcagtgag gattgtaact gttaagcagg caacaccttg
     3901 atgatattaa tgaataaata tgtgaggata tttgtttttt gatatgattg aggtgttttt
     3961 ttatttatgg gtaagcaggt gagagaatga gattaaaaaa agcatttaat aacaatgctc
     4021 ttctagcatt ggatgaaaat gaagaagaag tcattgtgat ggggaaggga atcgcttttg
     4081 gaaaaaagag tggagatagg attgatgagt cgttgattca aaaaaagttt gtttttgata
     4141 aaagtgaatt aaatgaaaag ttttctcaat tgtttgatga aattccacag caatatgtcg
     4201 aattaactgt atcaatagtt gaaatggcgc aaaaagaatt gggtgtaaga tttgattcaa
     4261 gtatatattt agctttatct gatcatatta catatgctgt tcgtcgttat aaaaagaatg
     4321 aaaatttaaa aaatgcatta ttgtttgagg tgaaaaagtt ttatccaaat gaatttaaag
     4381 cggctttaaa atccttagat atgattcaat atgaaacggg atgtattatg actgaagatg
     4441 aggctggata tatagcaatg cattttgtga atgcacaaca aaatggagag gaaatgtcac
     4501 aaactgttaa agtgacaaaa atggtagaag atattctgca tatagtggaa taccattatc
     4561 aaataaaatt ggatgaaaac tccttaaatt atactcgttt tgttacgcat attcgatatt
     4621 ttgcgcgtag attgtttgcg aaagaattga atcaagatac ggatgacatt ctttatcaac
     4681 aaattagaga acgttatcca gatgcttatc gttgtgtttt aaaagtaaag aagtatatca
     4741 aagagaattg tgacatagaa ttaacgaatg aagaaatgac ttattttatg ttacatatta
     4801 atcgtgtttg ttctagaatt aaaagttagc tttcaggctt gtaactgtta ggcaggcaat
     4861 aacctgagct tatgaatgat ttcgaaggga ttcgttgtct ttttcatatg ctcaggtttt
     4921 tatttttggt agtaactgtg caatcagtct acatatatta gaaattgaaa gattatggta
     4981 ccaatcttaa aatttatcaa aaatcaggag gaaattatga ataagtatga aactatagcc
     5041 aatgaagttg taaaaaatat tggcggaaca gaaaatatag aatctgttac aaattgtatg
     5101 actcgtttaa gatttacatt aaaaaatgtg tctaaggtag acgtagaatc tttagaaaaa
     5161 gtaaaaggtg tacaaggtgt tgtaaataaa aatggccaat tccaagtagt tattggatcg
     5221 gatgtatcac atgtatgtga tgaaataaaa aaaatgggta gcttttctga aactgtaaat
     5281 gcaaacagtg aagaaaacgt atctttagta aatcgcgtat taggtgcaat cacagctatt
     5341 ttccaaccag taattcctgc tatttgtggt gcaggtatga tcaaagctgt attagcgtta
     5401 ttgacagcat ttcatgttgt ggaagctact tcacaaactt atgttttatt agctatggta
     5461 gctgattcag cattctattt cttacctatt ttcttggctg tttcaagtgc taaaagattt
     5521 ggatgtaata catttattgc tgctgtctta ggcgctatgt tgattcaccc aacttatgct
     5581 ggaattgttt ctgctggaga agcttttaaa tttattggtt taccaattaa acttgtttcc
     5641 tatggaagta gtgtagtgcc agcaattcta attgtttggg ctcaatctta tgtagaaaaa
     5701 tttgcaaaga aaattagccc taatgcagtt aaagtattct tagttccttt aattgtattc
     5761 ttgattatga cgccattggc atttgttgta attggtccat taggttcatt tgttggtgat
     5821 attttatgta atatttttga tttcttaaat aatcaagctc gttgggtaat tccggtatta
     5881 atgggtgctt tctgtccatt gtttgttatg acaggtatgc attatagttt tatgccagtg
     5941 caattggcac aatatgcaac attaggatat ggaacgttgt taggtcctgg tatgttagca
     6001 agtaatattt cgcaagcagg tgcatgtttt gcggtggcat taagaacaaa agacaaagaa
     6061 atgaaatcaa cagcattatc tagtgcaaca acagctttat ttggaattac tgaaccagcc
     6121 ctttatggtg ttactatgaa attgaaaaag cctctttgga cagttatgat cagtggtggt
     6181 attgctggtc tttggggtgg actaacaaat atgcgtacat atgcaagtgc tacagcaggt
     6241 gtaacggctt taccagtata catttgtgat gatttatcta atgttagaaa tgcatttatt
     6301 tgtattgtga tcgcattagt aagttctttt gtattaactt tattgtttgg cgtgccaaaa
     6361 aatgaagata cttcttctga taacgaagtt tttgaggtgg ataacacaaa attgaataaa
     6421 tatactgaaa ttgtttcttg catgaatggt aaggttgttc ctttagaaaa tgtagaagat
     6481 gatgtttttt ctaaaaaaat catgggtcca ggtgtagcaa ttcttccaag tgaaggaaaa
     6541 gtatatgctc cttttgatgg agttgtaaat actgttttcc ctacaaaaca tgcatatggt
     6601 ttaactagtg tagatggaat tgaaacgatt attcatttag gattagatac tgtgaattta
     6661 gaaggtaaat attttgatgc taaagtgaag agtggtcaaa gcgtaaaaaa aggcgatttg
     6721 ttagctgaat ttgatttaga ggcattacaa aatgaaggct ataatatggt aacgcctgtt
     6781 attatcacaa acagtgatca atttattgat gtaattgaaa cacaaaaatc agatgtaaaa
     6841 gtaggcgatg aattagtgaa agtatttcgt taaaaaatag aaagtcaggt tttaaaatga
     6901 aatttcctaa ggagttttta tggggtggcg caacggcagc caatcaatgt gaaggtgcat
     6961 ggaacgaaga tggcaaaggt gctagtattc aagattatat gccacatgga attatgcaag
     7021 gacctagtga agtcattgat gatcataatt taaaattaaa aggaattgat ttatatcatc
     7081 attataaaga agatattgct ttatttgctg aaatgggatt taaagtatat cgattgagta
     7141 tagcatggtc tagaattttc ccaaatggcg acgatgaaaa acctaatgaa aaaggattag
     7201 aattttacga caatgtattt gatgaatgta aaaagtacgg aattgaacca ctagttactt
     7261 tatctcatta tgaaacacct tattatttgg caaaaaaata tgacggctgg aaaagtagag
     7321 atttaatagg attttatgaa aaatatgttc gtacagtatt tactcgttat aaaggaaagg
     7381 ttcattattg gattacattt aatgaaatta atagtatttg gcatttccct ttgatgggtg
     7441 ctggcatttt aacaccaaaa aatttattga atgcacaaga tttatatcaa gcagcgcatc
     7501 atgaattagt agctagtgca ttagcaacta aaattggtca tgaaattgat tctgaaaata
     7561 aaattgggtg tatggtttta ggactaacaa gttatccaag aacatgtaat cctgatgatg
     7621 ttatcgctac tatggaagaa agtaaacgtg gatatttttt tacagatatt catatgcgtg
     7681 gatattatcc ttcttatgct ttaaaaatga tggaaaaaga aggcgtggta cttgatgcta
     7741 cggatgaaga tttagaaata ttaaagaata catgtgactt tttatcgttt agttactata
     7801 tgtcgaaatg tattgcttca aatcctgaac aatatgaaaa aggtaaagga aatttaacta
     7861 caggggtaaa aaatccttat ctacaggaaa gtcaatgggg atggcaaatt gacccaaaag
     7921 ggttaagata tttgttgaat acatattatg atcgttatca aaagccattg tttattgtag
     7981 aaaatggctt gggagcaaag gatacattgc tttcagaaga aaaagatggt tattgggtag
     8041 aagacgatta tagaattcag tatatgaatg atcatttaac acaagtttca aaggcaatca
     8101 acgatgatgg tgtagaagtt atgggatata catcatgggg atgtattgat ttaataagtg
     8161 catcaactgc tgaaatgaaa aagcgatatg gttttattta tgtggatcga aataatgatg
     8221 gtactggctc atttaagcga tataaaaaga aaagctttta ttggtataaa aaagttatag
     8281 aaacgaatgg acaaattatt agctaagtga atgaattttc atttagcttt ttttaattta
     8341 ataattgata aaaatatgct gtgacgtaat tgactatttg aataatgtgc tataatttta
     8401 actaattggg ggagggatat gcatgacgtt aaaagaacta aaaataggac agagtgcatt
     8461 gataaaggcc gttggaggaa cgggtgcatt aagacaacac tttctagata tgggagtcat
     8521 acctggagct gaggttacag ttgtaaagtt tgcgcctatg ggcgatccaa tggaactgca
     8581 aattcatggt tatgaattgt cgcttcgttt agatgaagct gaaaaaatat tggttgaatt
     8641 aattgatgag agaacacgaa agcatgagag tgcagaaaat ataaataata cagttcaccc
     8701 tggacttggt gaagatggta aatatcatgt aaaggcagat gaacatcctt tgccagaagg
     8761 aacatgtttg acatatgcct tggtaggaaa tcaaaactgt ggaaaaacca ctttgtttaa
     8821 ccaactcacg ggatcgaatc agcatgtagg aaattttccg ggtgtaaccg tcgatcgaaa
     8881 ggatggtcca atcaaagggt atccaaatac aatggtgaca gatttaccgg gtatttattc
     8941 gatgtcacct tatacgagtg aagaaattgt atcgcgtaat tttgtgttaa atgataagcc
     9001 taaggctatc attaatattg tagatgctac aaatattgag cgaaacctgt atttgactat
     9061 gcaactttta gaaatgaata ttccgatggt tgttgcactt aatatgatgg atgaagtggc
     9121 caacaatcaa ggttcgattg atatcaatgg tatggaggct atgcttggtg taccagttat
     9181 acctatttct gcagctaaaa accaaggtgt tgatgaattg attgaacatg cgattcatat
     9241 tgcgaaatat caggaaagac caggccgttt agatttttgt ggggaagatg attttggtgg
     9301 agccgttcac cgttgtatac attccatctg tcatttgatt gaagatcatg ccaaaaagac
     9361 agatgttcct ttgcgttttg cggctagtaa aattattgaa ggcgataatt tgattcttga
     9421 tcgtttggac ttagatgaaa atgaaaaaga aatgattgaa cacattgtgc ttcaaatgga
     9481 aaaagagcgt ggactcgatc atagtgcggc cattgccgat atgcgttttt cttttattga
     9541 aaaagtttgt gaacaaaccg ttgtaaaacc taaagaaagt aaagagcgtg ttcgaagtga
     9601 aaaaattgac cgcgttttaa cgggtaaata tactgcaatt cctatgttta ttggaattat
     9661 gttacttgta ttctatctaa catttaatgt agttggagca tggcttcaag gattgttaga
     9721 attcggtatt gattggatca cacaagtagt ggatgcatgg atgacaagtg ctcacgtaag
     9781 ttatgctgta catagtttag tgattgatgg catctttgcg ggtgtaggtt ctgtcttatc
     9841 tttcttacca atcattgtga cactattctt ctttttatct atgatggaag atagtggata
     9901 tattgcgcgt gtagccttct ttatggataa actattaaga aagattgggt tgtcaggaag
     9961 aagtatcgtt cctttattga ttggatttgg atgtacagta ccagccgtta tgtcaacacg
    10021 tactttgcct agtgaaagag atcgtaagat gacgatttta ttgacgccat ttatgtcttg
    10081 tactgcaaaa ttaccaattt atgctttctt tgtgaatgca ttctttcgaa aacaaggtgg
    10141 tctagttatg acaggacttt atgttttggg cattgccgta ggtatattga ttgcgttctt
    10201 atttaaaagt actttattta aaggggaagc tgttccattt gtgatggaac ttccaaacta
    10261 tcgtttgcct ggattaaaga atgtgagtca attgttgtgg gaaaaggcaa aggatttctt
    10321 acaaaaagcc tttacggtta tttttgtggc aactgtactt gtgtggttcc tacaaagctt
    10381 taatttacaa ttcgatatgg tcaaagattc gagtcaaagt attttggcaa tgattgccgg
    10441 atggattgct cctgtatttg cgccattagg acttcaagat tggcgtattg taacgtcctt
    10501 aatcagtgga tttatggcta aggaaagtgt tgtttctaca ctacaagtat tatttgtagg
    10561 tggtgtacat agtgcattaa ctacactatc agctgcatcg ttacttgtat tctgcttgat
    10621 ttattcacca tgtgttgcgg ctatcgcatc tattaaacga gaactcggtg caagatgggc
    10681 aattggtgta gttgcatggc aatgtgtact tgcatggatt ttagccttta ttgttcatgt
    10741 gattggttct gtattataag tagctttaat gctacttttt ttttgaaaga agatgtttta
    10801 taattttttc atatctaagg gaggatgttt acttatgtat aagttaattg cgtgtgattt
    10861 agatgaaact ttgattaaac ccgatcgaac aattgattct agagatattg aggccattaa
    10921 gaagttgaaa gatttaggtg ttaaatttgt actggctaca ggaagaggtt ataacacagt
    10981 tcagggtaca ttaaaggaat tggacctttt cgatgaaacg aaccaatatg tgatttcata
    11041 taatgggggt gctattactg aaaataagga caatcgactt ttacattttg aaggtatttc
    11101 ttttgaaaaa gctgaagaat tatataagcg tggattgaat tatgatgtag tcattcatgt
    11161 atatacgcgt gatgcggtat atgcatataa cttaacggaa gatgaaaaaa gatatgtgca
    11221 gaatcgtcag gaaatgattg aggtatttga tcagaatctt gattttttaa agggtcaaga
    11281 aattgtaaaa gtattatata caaatacaga ttttgagtat cttcaaagta ttgatcgtga
    11341 tctaaaggat attacgtatg atatggatgt ttcttattca agtaatcgtt atattgaatt
    11401 taatcataag ggtgtaaata agggggctgg attaaaaaaa ctagccgatc tattacatat
    11461 tgatattcag gatacgattg cgattgggga taactataac gatttatcta tgatcaagga
    11521 tgctggttta ggtgttggag tacaaaatgc tgcacctggt attaaaccag attgtgacta
    11581 tatcacaacg gctacttgtg aagaaggtgc tgtttgtgaa gttattgaaa aatttgtatt
    11641 aaaaggagag taaaatgaag aaacttattc aattatatgt ttcttggttt aagatgggtc
    11701 tatttacttt tggtggtgga tatgccatgt tgccaatgat tcaaaaggaa gtgattgaaa
    11761 agtatcattg ggctacagaa gatgaagtca tggactatta tgcgattggt caatgtacac
    11821 ctggcgttat tgcggtcaat acatcgacat ttattggata ttatcaagct ggagttccag
    11881 gtgcgattgc agctactcta ggtgtagtta ctccttctat tatcattatt agtataattg
    11941 caggtttgat cacaaacttt tcaagtttag ctatcgtgca acatgctcta aatggaattc
    12001 gtgtagctgt atgtatgttg atgattaatg cggtattgaa actaggtaag ggtaatatta
    12061 aaaataagat tggtttcttg atttttgcgg tggcccttat ttttgcgatg tttactaaag
    12121 tatctactgt actacttgtg attggtgctg gtattgcggg tgttgttttc tctagaatag
    12181 gatggttaaa gaaaaatgac taaattgtta ctcatgtttt tagaattttt caagactgga
    12241 ctatttgcgg taggaggcgg tcttgcgacc attcctttct taagagagat tggagttcgt
    12301 tatggctggt atactatgag tgatctatct acaatgattg cggttagtga atctacacca
    12361 ggtcctatgg gtgtcaatat ggctagttac gtaggcttta gtcaatatgg tgtattaggt
    12421 tcgattgtgg taacacttgg cttggtttta ccaagtctga ttattatttg tatcattgcc
    12481 aatttcttag agaaatttaa agaggcgaag cttgttcaac aaatctttgc cggattaaaa
    12541 cctgctgttg ttgcctttat tgccgtggct acaattgata ttttggtgac aactttattt
    12601 ggtgattata ctttctcaac atttcactgg aaacaattta tttgcctatt catattattg
    12661 gcgttgagta agtataaacc taaattacat ccaatctggt ttattgtagc tagtgcggtt
    12721 gtaggaattg tatttgcgtt ttaagtcata gggaaactta tggctttttt cttttaggta
    12781 aaataaaaag caatgtctgg gcttgacatt gctttaggga gaggaaaatt attaagataa
    12841 aaatgtatct tgagtttata gggttgaata attttccaaa gggttaatca taagataagg
    12901 catttcttat gacacttaaa atatatattt ttttagtact taacgcaatg aacacatatt
    12961 ttgatttgta tattattatg tgtgtaatct tgttgtagtt tgtgttaagc tactaagtag
    13021 aaggagatga ttttaatgtg taccgcatta agctttaatc agttttttgg aaggaattta
    13081 gattatgaat tttcatatgg agaacaagta gctgttacgc cacgcaatta tgattttcat
    13141 tttagacatt tagatcaaca tgaaagtcac tatgctttga ttggtatggc ccatgtattt
    13201 gaagagtatc cattatatta tgatgccatg aatgagaaag gcttgggaat ggctggatta
    13261 aactttgttg ggaatgcaaa gttttatgaa gtcaaagaag acaagaacaa tgtggctgcc
    13321 tttgaattta ttccttggat cttatcacaa tgtgcaagtg tcaaagaggc aagagtcgta
    13381 ttagaaaata caaatgtttg tgccacacca tttaatgaac actttccagt agctgaatta
    13441 cactatatga ttagtgatga aaatgaaagc attatcgttg agtgtatgga agatggtatg
    13501 catgtatatg ataatcctac gcatgttttg acaaataatc caccatttcc tatgcagtta
    13561 tttcgtttga atgattatat gtatttatca aataaacaac ctaaaaataa ttttggcttg
    13621 gacttaactg cctattcacg tgggtttgga gctatgggac ttcctggaga cttatcgagt
    13681 ggatctagat ttgtgcgtgt tgcctataca agagcaaatg cgacaagtga caagaatgat
    13741 ttaaatcaat atttccacat tattggtagt gttacgcaac aaaaaggtct ttgtgaagta
    13801 gaaccaggta agtatgaata tacaatttat acgtcttgtt gcgacttaag aaatggaatt
    13861 tactattata cgacatacaa taatcatcag attagtggtg taaatatgtt taatgaaaat
    13921 ttggatagtg aagtacttat ttcgtatcca atatttgaag aagaaatgat tcatatgcag
    13981 aattaatttc ttgatgacat gaacttttat aggttatgtt ttgtgtaaaa gagaagaaag
    14041 aaagcctact ggaagtggtt ccatgtaggc ttttctaata gttcataagc tgtttttgcg
    14101 tttctgatat tggtgttcga gaatttatca aataatttat aattgtttaa tct
//
LOCUS       DBMDWL010000036        14122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1457_length_14122_cov_3.009985, whole genome shotgun sequence.
ACCESSION   DBMDWL010000036 DBMDWL010000000
VERSION     DBMDWL010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 14122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14122
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_14122_cov_3.009985"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(35..310)
                     /locus_tag="ACZPDN_03940"
     CDS             complement(35..310)
                     /locus_tag="ACZPDN_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297806.1"
                     /translation="MDQKDRVFTPTFIANLFLIAGTVFLFLKGKINAYVGYDGAMHDG
                     NALMLIGLALVCIGIGIWIIVWINKRMNRYKVAPVSRTINKKGLKIK"
     gene            437..661
                     /locus_tag="ACZPDN_03945"
     CDS             437..661
                     /locus_tag="ACZPDN_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HIR1297807.1"
                     /translation="MQNIDDLVELHECPLCEGGSILEEEGNGFYAQCMDCGCYTVHVD
                     YKNEEDRLEAAKRAAELFNTGKVLKSNPGE"
     gene            complement(692..2059)
                     /locus_tag="ACZPDN_03950"
     CDS             complement(692..2059)
                     /locus_tag="ACZPDN_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297808.1"
                     /translation="MNMMEITAQMRENKKIEFKDQLKIVILLSVPAILEQLVGTAMSY
                     IDTAMVGSLGYKATAAIGVVASTTWLFGGICTAAVLGFSVQVAQYLGAGKNKLARQVV
                     CQSILFNIIFGLCMALIVVGASFYLPTLLGADPSICKDATLYLAIVGAFIPFNMMAML
                     HSGMLRCSGNAVLPSLMNISMCVFDVLFNYFFIYILNLKVAGAALGTGMAQLVVGFIL
                     MYIVVKKESSLRLLGDESWKFDKDILKNVVNLSIPAGLERVTLSLAQVVMTGVVSSMG
                     AVSVAINYVAVSAEGLCYLPAYGIGGAATTLVGQSIGAKRKDMAKRFAYLTTLLSFVL
                     VIVMSVILFAFAPFLASTLTNSQEVIDGASECLRIVSFSEPLFAISIVVMGALRGAGD
                     SKRPFVLNALSMWGMRVLPIIIFTKRYGVIGVWVTMTFELVFRGILFFIRMVRGKWLN
                     QNSIE"
     gene            complement(2103..3371)
                     /locus_tag="ACZPDN_03955"
     CDS             complement(2103..3371)
                     /locus_tag="ACZPDN_03955"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1297809.1"
                     /translation="MEKVIKIEGGHHLNGTVRISGSKNATVALIPACVLGNEPVTIYG
                     VPNISDVQSLIVLLNELGVCVEKRDEETLYIDPTHMENIPMVSKAVSKLRASYYFMGA
                     LLGKFGHAEIKMPGGCYLGPRPIDLHLKGFEALGADIQYENGCYILDAKELKGTNIFL
                     DISSVGATINIMMAAVYAKGRTVIENAAREPEIIDIATLLNKMGARIHGMGTSTLVID
                     GVDYLKGCIHEIIPDRIEAATYVIMAAAMANDMRIENIIPQHLEAIVMKLQEVGINME
                     VGSDFIHVFKSDKPLKRTEILTKPYPGFATDVQQPFTVLLTQCEGQSVVTETIYTERF
                     KHCAELNKMGANIDVHTPSAFINGKTKLHGSKVTATDLRCGAGLVIAALMADGVTEIH
                     DIYHIDRGYDNLDGKLAHLGAKMWREEVED"
     gene            complement(3462..5315)
                     /locus_tag="ACZPDN_03960"
     CDS             complement(3462..5315)
                     /locus_tag="ACZPDN_03960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR1297810.1"
                     /translation="MKSIEINDLLSYHFYGNLKVKDGVKVFVDGVAAEKDNAYDYTLK
                     CVKDDKVYDLTSYGKESGFYIENENSILFCGNRKNIEKSTSLYRLSLNGGEAKEIARF
                     DKPGMSVLGYLNEKTLVLLTKEKLVEEESDYEVFDEIPFYMNGQGVTNKHRSHLYTYN
                     LDTKELVCVTEGTFDVSNAKIHDGTLYYTGQNWTRKQSLYENLYKYDGEVSTVVEAKE
                     RSIQSFDFVDDKLVLIASTHEKYGLNENPKFFDQDFNLIVDWQECVGNSVGTDCALVA
                     GNATLVKNQALYFVSTIYDHNNLYKLENGKIELVFEWPGTIQSFAFEDDTLYFIGADV
                     ASLQQLYKVQDGKVIQLSDFNKMEDTYVAYPVEVSFGENLTGWVLLPKDYDETKKYPA
                     ILDIHGGPKTVYGKVFYHEMQVWASKGYFVFFCNIHGSDGRGNEFMDIRGKYGTIDYD
                     ELMQFTDKVLETYPQIDTSKVGVTGGSYGGFMTNWIIGHTDRFACAASQRSIANWISF
                     SQTSDIGPYFAQDQQLATYYENPEKLWWHSPLKYARNVKTPTLFIHSDEDYRCPLCEG
                     LQMLNALLDQNIEARMCLFHGENHDLSRTGLPQHRLRRLNEITNWMEQHLK"
     gene            complement(5377..6246)
                     /gene="fba"
                     /locus_tag="ACZPDN_03965"
     CDS             complement(5377..6246)
                     /gene="fba"
                     /locus_tag="ACZPDN_03965"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1297811.1"
                     /translation="MLVSATEMINKAHEGHYAIGAFNINNLEWTKAILAAAEEAKSPV
                     MLGVSEGAAKYMCGFKTVAAMVKEIHDAMGITVPVALHLDHGSYEGAKAAIEAGFTSV
                     MFDGSHYAFEENLEKSKEMIALAHSKGLSIECEVGGIGGEEDGVISAGELADPKECKT
                     IADLGVDFLAAGIGNIHGKYPANWAGLNFDRLEEIQNVTNGKPLVLHGGSGIPREQVQ
                     KAITLGVSKVNVNTECQLVFAEATRKYIEAGKDQEGKGYDPRKLLKPGADAITALCKE
                     MMENFFGSAGKAE"
     gene            complement(6325..7227)
                     /locus_tag="ACZPDN_03970"
     CDS             complement(6325..7227)
                     /locus_tag="ACZPDN_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297812.1"
                     /translation="MNLNIKLSEEQMLERQRNIARLKENELIQLFLNQNHLDASFVEE
                     NSGALLQWLKSVSACRNCKGLKYCAQKIRGKVSKLKIDDSGFLNEYYASCQYEQNRDK
                     QLAHQSKFRFSHMSLNDYLIDLNDASFLSAAKEKEYGLAYTQSVSSIPLNQGVYLYGN
                     PGTGKSYLMKGICNYYAKSNKSVSFVQVPLLIQELKQFMTDNEYRQRVMNHLRYSDVL
                     VLDDIGAESITQWTRDEILLPILDERMNKGMKTYFTSNYSMEELEQQYRLANKPNNTI
                     ASLRILERIRTLSKPVELSGKSRR"
     gene            complement(7227..8360)
                     /locus_tag="ACZPDN_03975"
     CDS             complement(7227..8360)
                     /locus_tag="ACZPDN_03975"
                     /inference="COORDINATES: protein motif:HMM:NF018914.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR1297813.1"
                     /translation="MAIEVRCIEHLSSEQRQSLNLLYGPLMGKNSICLYEFLGSIQNL
                     VELEDVYLLLNMNASQFDIARNRLEQYHLIETYVHEGDMLILLYAPLLPDSFLCHETY
                     SRLYLASVGAKCFDKVKAMLYKDKTVSSSYTKVESPLDVSILDSWNESKEIAFEKVKP
                     TIKQKYDFDFATLFKGMDRIFPVRLRTSENLDRIAEMAKIYGIDAKDMRKYVQRSTNP
                     STHVFDLEKLKDMVMRNRKVMEVSKDPYKMSPVKFLQNKQNGIPVVKSDQALIERLCK
                     EFQFPIEVVNTLIEYTLQQTNQQFSRNYVEKVAASWVRLGVDSRKKALDIINQSPLEN
                     KRDKKAEKVVLDDKFYTQKEEKSDAQLHKEMLELQKMLKEGKL"
     gene            complement(8350..8955)
                     /gene="coaE"
                     /locus_tag="ACZPDN_03980"
     CDS             complement(8350..8955)
                     /gene="coaE"
                     /locus_tag="ACZPDN_03980"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1297814.1"
                     /translation="MSKMSKKKVIGITGSMGSGKSEISRYLRKKYPVLDCDQVNADLL
                     KKGNLGYQKLNDLHIVELDSNGEIIKESLASYMFSNEEHRKQVEAILHPLIFDEMHKW
                     IHEQESLTVFVEIPILFEISAQEHFDSIWCVVADLDVALSRLQTYRNFTREQALARLV
                     SQMNPEEKMAKSDIVLRNNSTVEQLHMQIEDALKEEKLNGD"
     gene            complement(8928..9749)
                     /gene="mutM"
                     /locus_tag="ACZPDN_03985"
     CDS             complement(8928..9749)
                     /gene="mutM"
                     /locus_tag="ACZPDN_03985"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HIR1297815.1"
                     /translation="MPEAPEVQTVLSTLETQIKNISIEDVLIYYPKIVDNVEVETFKH
                     RLIGQSFRTFNRLGKYLIFGLDDYDLVVHLRMEGKFYLYDKICEDKHIHVVFKLNNGI
                     CMSYHDTRKFGRMYLYSKNDDIHSYACFKNVGYDYMDPRVNGMYFYHCIHTLKRNLKS
                     CLLDQSIMAGIGNIYADEICFALGLDPRSRACKLSKKDCEKIVLETRRILQGATHYGG
                     TTIRSYTSSLGVTGRFQLHLKVHGQAQCKVCHHTIKKLTVSQRGTYLCPKCQKRK"
     gene            complement(9749..12325)
                     /gene="polA"
                     /locus_tag="ACZPDN_03990"
     CDS             complement(9749..12325)
                     /gene="polA"
                     /locus_tag="ACZPDN_03990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR1297816.1"
                     /translation="MKKLLLLDGNSMLFRAYYATLYTHRMTTSNGIPTNAVYGFVMML
                     NKAIDIIEPDKILVAWDAGKPTFRHKQFAAYKGTRKPLDEELIVQFPIVREYLDAAGI
                     KRYEQEGYEADDIIGSMAKCCNDIQTTILTSDRDLLQLIDPSTHVLLMKKGLSEMELM
                     DEQNLFDTYGITPSQVIDMKGLMGDTADNIPGVQGVGEKTALRLLNQYSTVENVYAHI
                     DEVKGKLKEKLEKDKGNAFMSLELATIYTKMELPFSLNDCEFTGIQEDVNGFYEKYEM
                     RSLVNRTKQAKNEKWPLKEVEHFEMFDVEDLMVLPVCTQQPYLDQQLYGFMIPKDKVI
                     YYISVENALEDTNFKKILETKEISTWDTKEMMHLLDRYGFKWNTFNDDLHIAGFLLNS
                     TATDSDAVLEALHITLPESFHDVSKKTKEEPAFSLNREQALCHSLTQQLYEKKGEILQ
                     SLKEENVLSLYREIEMPLTRVLFSMEQEGISIQESFLDEYGALLNEKLEILAQQIYSH
                     AGMIFNINSPKQLANVLFDELNLSSGGKKRSTSADVLEKLRGKHPIVDDILEYRKISK
                     VSSTYIDGLKKHVRSDQKIHTSFNQTMTQTGRLSSSEPNLQNISIRDELGKEIRKAFV
                     AQEGYHLLSADYSQIELRMLAHMANEDHMIKAFNEGLDIHTKTATLIFNCSPEEVNEH
                     KRRIAKTVNFGIVYGQTEFGLSSQLNITRKEAGEFMKMYFESYPKIHEYMNQLIDFCK
                     ENGYVETLFHRRREIPEINDKNFMTREFGKRAAMNAPIQGSAADLIKIAMLKMDQALK
                     DANVNSKMLLQIHDELIFLVPDEELDLMQKLVKETMESAMELKVPLKASISVGKSWYE
                     AK"
     gene            complement(12385..12951)
                     /locus_tag="ACZPDN_03995"
     CDS             complement(12385..12951)
                     /locus_tag="ACZPDN_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297817.1"
                     /translation="MINKERCSFIFLLILAILDQMNASLFGVDFTYSSLSFVSHLCFC
                     GCILVVSHEKSLNRLLYACLCGLCVGLFFTSDWLVKMVLFGFFGWLSGLFKNAMDQDH
                     WVQFALVFLMMFINDLICFLGAKLFGYLTMSFVKWFIHVEALTLIFNGAWIFVLLYII
                     TVFNRYATIKNIRFKRMEKLHMQNIRRK"
     gene            complement(12948..13784)
                     /gene="mreC"
                     /locus_tag="ACZPDN_04000"
     CDS             complement(12948..13784)
                     /gene="mreC"
                     /locus_tag="ACZPDN_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1297818.1"
                     /translation="MRKFTRIQKRLLTIIVVLCILACAMFGLRQNSITNIGYSAWTYL
                     KYGLIQYPLSSIGNAVNDFSNLWHAYDDNEYLTKQLAQQKNYQTAYEDERNKNQQLEA
                     LLEMKGGLGDAKTISVSVLERSGDSWLQTVTISAGKRQGVEKNMLVATSDGAIGLVDS
                     VQTSTSTVQLLTSQHLNNDIAIKMSLEDGSSVEGVLQSYDTEKKAYRVSLFDNDAIVT
                     KGQKVATSGKGGNYPSGILIGEVKDVSLNDDSIISTVYVSPVSNMKSFDYCLVIGKEE
                     SK"
     gene            complement(13832..>14122)
                     /locus_tag="ACZPDN_04005"
     CDS             complement(13832..>14122)
                     /locus_tag="ACZPDN_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015935.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR1297819.1"
                     /translation="ASLNTSRGCTTLLFKGTLNESVVHPREVFKEAFLQNANSVLIAH
                     NHPSGDCTPSKADFEVTYKMVHVAMTMGVCLVDHIIVCKNQYYSFKEHKYLD"
BASE COUNT         4500 a         2925 c         1870 g         4827 t
ORIGIN      
        1 tccggtattt gacacctaga ggatatattt atttttattt aattttaaga ccttttttat
       61 ttattgtcct tgacacaggg gccactttat aacgattcat cctcttatta atccatacta
      121 tgatccaaat gccaatgcca atacatacta aagccaagcc aatcaacatt aaagcattac
      181 catcatgcat cgcaccatca tatcctacat atgcgtttat tttccccttt aaaaacaaga
      241 aaacagttcc tgcaattaaa aataaatttg cgataaatgt aggcgtaaaa acacgatcct
      301 tttgatccat aataacatcc ccttattgtt accgatgtat tatatcattt cattccttta
      361 ttaaacctta aatttctctt aactttctag acatttgatt gtacaataga taagaataaa
      421 agaaggaagg attcttatgc aaaacattga tgatttagtt gaattacacg aatgccctct
      481 atgcgaaggt ggttcgattc tagaagaaga aggaaacgga ttctatgcac aatgcatgga
      541 ttgtggctgc tatacagttc acgttgatta taaaaacgaa gaagatcgtt tagaagcggc
      601 aaaaagagct gctgaactct ttaatacagg taaagtatta aaatcaaatc ctggtgaata
      661 agaaaatgag tggaattcca ctcatttttt tctactcaat tgaattttga tttaaccact
      721 tacctcgaac catacgaata aagaatagaa tacctctaaa taccaattcg aaagtcattg
      781 taacccatac accgataaca ccatatcgtt ttgtgaagat gataattggt aaaacacgca
      841 tgccccacat actcaaagca tttaagacaa aaggacgttt actatctcca gccccacgta
      901 atgctcccat gactacaata cttatcgcaa ataatggttc tgaaaaagaa acaattctta
      961 agcattcact agctccatct atgacttctt gactatttgt cagagttgaa gctaagaatg
     1021 gcgcaaacgc aaatagaatg acactcataa ctatgacaag gacaaatgat aataaagtcg
     1081 ttaaatacgc aaagcgtttc gccatatctt tgcgctttgc accaatactt tgtccaacca
     1141 atgtagttgc agcaccacca attccatagg ctggcaaata acataaccct tctgcagata
     1201 cagctacata attgatggcc acactcacag ctcccatact tgaaacaaca cctgtcatta
     1261 caacttgagc taaagagagc gtgactcttt caagccccgc aggaatcgaa agattaacta
     1321 cattctttaa gatatcctta tcaaacttcc aagattcatc tcctaaaaga cgtaacgaac
     1381 tttccttttt gactactata tacattaaaa taaaacctac aaccaactgc gccattcccg
     1441 taccaagtgc agctcctgca acttttaaat tcaatatata aataaagaaa tagttaaaaa
     1501 gaacatcaaa tacacacatt gaaatattca taagacttgg aagcacggca tttcctgaac
     1561 atcttaacat tcctgaatgt aacatagcca tcatattaaa tggtataaaa gcgcctacaa
     1621 tagctaaata taaagtggca tctttacata ttgatggatc agctcctaat aaagttggca
     1681 aataaaaact ggctcctact acaattaaag ccatacataa gccaaatata atattaaaaa
     1741 gtatcgactg acaaacaact tgcctagcca gtttattttt acccgcccct aaatattggg
     1801 ctacttgcac tgaaaaacct aatacggctg ctgtacaaat accaccaaat aaccatgtag
     1861 tactggcaac tacaccaata gctgccgtag ccttatagcc taaagatcca accatcgccg
     1921 tatcaatgta actcatggct gttccaacta attgttctaa tatggcaggt acagataata
     1981 aaatgacaat ttttaattga tctttaaatt ctattttttt attttcgcgc atctgcgccg
     2041 ttatttccat catattcata aattgattat acatagaaaa aagatgtatg tcaccataca
     2101 ccttaatctt ctacctcttc acgccacatt ttagcaccta aatgagctaa ctttccatct
     2161 aagttatcat atccacgatc aatatgataa atatcatgaa tttctgtaac accatctgcc
     2221 atcaaggctg cgatcacaag tcctgcacca caacgaagat ctgttgcggt tactttcgaa
     2281 ccatgtaact ttgtttttcc attgataaat gcacttggtg tgtgtacatc aatattcgca
     2341 cccatcttat ttaattccgc acaatgctta aatcgctctg tataaatcgt ttctgtgaca
     2401 acagactgtc cttcacattg cgttaataat acagtaaacg gttgctgcac atcagtcgca
     2461 aatcctgggt atggtttagt caatatttct gtgcgtttta atggcttatc actcttaaat
     2521 acatgaataa aatctgaacc cacttccata ttgattccca cttcttgaag tttcataaca
     2581 atggcttcaa gatgttgagg aataatattc tcaattcgca tatcattagc catagctgct
     2641 gccataatca cataagtagc tgcttcaata cgatccggaa taatttcatg aatacatccc
     2701 tttaagtaat caacaccatc aatgactaat gtacttgtac ccataccatg aattcttgcg
     2761 cccattttat tcaataaagt cgcaatatca ataatttccg gttcacgtgc cgcattttca
     2821 atgacagttc ttccttttgc gtaaacagct gccatcatta tattgattgt agctcctaca
     2881 cttgagatat ctaagaaaat atttgttcct tttaattctt tcgcatccaa tatataacat
     2941 ccattttcat attgaatatc tgcacccaaa gcttcaaagc ccttcaagtg taaatcaata
     3001 ggtctaggac ccaaataaca gcctcctggc attttaattt ctgcatgtcc aaattttcct
     3061 aacaaagctc ccataaaata gtagcttgct cttaattttg aaacagcttt agataccata
     3121 ggaatatttt ccatatgagt aggatctata tataatgttt cttcatctcg tttttcaaca
     3181 caaacaccaa gttcattcaa taaaacgatt aaagactgta catctgaaat attaggtaca
     3241 ccataaattg tcactggctc atttcctaaa acacatgctg gtattaaagc cacagtcgca
     3301 tttttagaac cactaatacg aaccgttcca tttaaatgat gtccaccttc aattttaatg
     3361 actttttcca ttcattttcc cccatttcaa ggtaatgcct atctattata tcctattcaa
     3421 tccggataat ctagaaaaat attgtaaatt ccaaaagcca tctattttaa gtgttgttcc
     3481 atccaatttg tgatttcatt taaacgacgc aaacgatgtt gtggaagtcc cgtacgtgat
     3541 aaatcatgat tttcaccatg gaataagcac atgcgagcct caatattttg atctaataat
     3601 gcattcaaca tttgtagacc ttcacaaagc ggacaacgat aatcctcatc cgaatgaata
     3661 aacaatgttg gtgtctttac attacgcgcg tatttcaatg gtgaatgcca ccataatttt
     3721 tctggattct cataatatgt agctaattgt tgatcttgtg caaaatatgg cccaatatca
     3781 cttgtctgag agaatgaaat ccagtttgcg atacttcttt gactagctgc acacgcaaaa
     3841 cgatctgtat gaccaataat ccagtttgtc atgaaaccac cataacttcc tccagtcacg
     3901 ccgactttac tagtatcgat ttgaggatat gtttccaaca ctttatctgt aaactgcatt
     3961 aactcatcat aatcaatcgt tccatatttt ccacggatat ccataaattc attgccacgt
     4021 ccatctgatc catgaatatt acagaagaac acaaaatagc ctttacttgc ccatacttgc
     4081 atctcatgat agaacacttt tccatataca gttttaggac caccatgaat atctaaaata
     4141 gccggatatt tttttgtttc atcataatct tttggcaaca atacccatcc tgttagattt
     4201 tcaccaaaac ttacttctac tggatatgcc acatacgtat cttccatctt attaaagtca
     4261 gataattgaa tcacttttcc atcttgaacc ttatataatt gttgaagact cgcaacatct
     4321 gctccaataa aatacaatgt gtcatcttca aatgcgaaag attgaattgt tcctggccat
     4381 tcaaatacaa gttcaatctt tccattttct aatttataaa gattattatg atcataaatg
     4441 gttgatacaa agtataaagc ttgatttttt actaaagtcg catttcctgc aactaaagca
     4501 caatctgtac ccacgctatt accaacacat tcttgccaat ccacgattaa attaaagtct
     4561 tgatcaaaga attttggatt ctcattcaaa ccatattttt catgtgtact tgcgatcaaa
     4621 actaatttat catccacaaa atcaaaactt tgaatacttc tttccttcgc ctctacaact
     4681 gtacttactt caccatcata cttataaaga ttttcatata aagattgttt tcttgtccaa
     4741 ttttgacctg tataatacaa agtgccatca tgaatttttg cgttggatac atcaaaagtg
     4801 ccttctgtaa cacaaacaag ttcttttgta tccaaattat atgtatataa atgactacgg
     4861 tgtttatttg tgactccctg accattcatg tagaaaggaa tttcatcaaa cacttcataa
     4921 tcactttctt cttccactag tttttctttt gtcaataata caagtgtttt ctcgtttaaa
     4981 tatcccaata cactcattcc tggtttatcg aagcgagcaa tttctttggc ttctccacca
     5041 tttaaagata aacgatataa agaagtggat ttttcaatat ttttacgatt tccacagaat
     5101 agaatcgaat tctcattttc gatataaaaa ccagattctt ttccataaga agttaaatca
     5161 tatactttat cgtcctttac gcattttaaa gtataatcat aagcattatc tttttctgct
     5221 gctactccat caacaaagac ttttactcca tctttcactt ttaaatttcc ataaaagtga
     5281 taagacaata aatcattaat ttcaattgac ttcatgtttt cccctccaaa ttatacaaaa
     5341 agcaggctta aagcctgctt tatttaaaaa ctttaattat tcagcttttc cagcagaacc
     5401 aaagaagttt tccatcattt ctttacataa tgctgtgatt gcatcagcac ctggttttaa
     5461 taatttacgt ggatcgtatc cttttccttc ttggtctttt ccagcttcga tgtatttacg
     5521 agttgcttca gcaaatacta actggcattc agtattaacg ttaactttag aaacacctaa
     5581 tgtgatagct ttttgaactt gttcacgagg gattcctgaa ccaccgtgta atactaatgg
     5641 ttttccgttt gttacgtttt ggatttcttc taaacgatca aagttcaatc cagcccagtt
     5701 tgcaggatat ttaccgtgga tatttccgat accagctgct aagaagtcta cacctaagtc
     5761 agcgatagtt ttacattctt tagggtcagc taattcacca gcagatataa caccatcttc
     5821 ttctccaccg attccaccaa cttcacattc aattgataat ccttttgagt gtgctaaggc
     5881 gatcatttct tttgattttt ctaagttttc ttcgaatgca tagtgagaac catcgaacat
     5941 tacagaagta aatcctgctt cgatagcagc ttttgcacct tcatatgaac cgtggtccaa
     6001 gtgtaaagct acaggaactg taattcccat tgcatcgtgg atttctttta ccatagcagc
     6061 aactgttttg aaaccacaca tatatttagc agcaccttca gatactccta acataactgg
     6121 tgatttagct tcttcagcag cagctaagat agcttttgtc cattctaagt tattgatgtt
     6181 gaaagcacca atcgcatagt gcccttcgtg agctttgttg atcatttcag tagcagatac
     6241 taacattact ctattcctcc tttaaacatt tctgtcataa ctattctact ccataacgcc
     6301 taatttgaaa agtgaaatta ttaactaacg acgtgatttc cccgataatt cgacaggttt
     6361 cgataaagtt cgaattcttt caagaattct tagactcgca attgtgttat ttggcttatt
     6421 cgcaagacga tattgttgtt ctaattcttc catggaataa ttcgatgtaa aataagtttt
     6481 cattccttta ttcatacgct catctaaaat cggaagtaaa atttcatcgc gcgtccattg
     6541 tgtaatactt tcagctccaa tatcatccaa aaccaaaaca tcactataac gcaaatgatt
     6601 catcactctt tggcgatatt cattatccgt cataaattgt ttcaattctt gaattaataa
     6661 aggaacttgc acaaaagaaa cactcttatt acttttagca taataattac agattccctt
     6721 cattaaatac gatttacccg ttccaggatt tccatataaa tacacgccct gatttaatgg
     6781 aatactactt acactttgcg tatacgcaag gccatattcc ttttctttgg cagcacttaa
     6841 aaaggaagca tcatttaaat caatcaaata atcatttaaa gacatgtgag aaaagcgaaa
     6901 cttactttga tgtgccaact gtttatcacg attctgttca tattgacacg aagcataata
     6961 ttcatttaaa aaacctgaat catcgatttt taattttgaa accttaccac gaattttttg
     7021 agcacaatac tttaatcctt tgcagtttct acaagcactc acacttttta gccactgcaa
     7081 caaagcacca ctgttttctt ctacaaaaga tgcatctaaa tggttttggt ttaaaaacaa
     7141 ctgaattaat tcattctctt ttaaacgtgc gatatttcgt tgtctttcta acatttgttc
     7201 ttcggaaagt ttaatattta aattcactac aatttacctt ctttcaacat cttctgcaac
     7261 tccaacattt ccttatgtag ctgtgcatct gatttctctt ctttttgcgt atagaattta
     7321 tcatctaaca caaccttttc tgccttttta tcccttttat tttcaagagg actttgattg
     7381 atgatatcca aagccttctt tctagaatcc acgcctaatc gaacccagct tgccgctact
     7441 ttttctacat aatttctaga aaattgttga ttcgtttgtt gcaaagtata ttcaattaat
     7501 gtatttacga cttctatagg aaattggaat tctttacaca aacgctcaat caacgcttga
     7561 tccgatttca caactggaat tccattttgt ttattctgca aaaatttaac tggagacatt
     7621 ttatatggat ctttactcac ttccataacc ttacgattac gcataaccat atcttttagt
     7681 ttttctaaat cgaatacatg cgtagaagga ttcgtacttc tttgtacata tttacgcata
     7741 tctttggcat caattccata tattttagcc atttctgcga tacgatccaa attttcgctt
     7801 gtacgtaaac gcactggaaa aattcgatcc atccctttaa agagtgtcgc aaaatcaaaa
     7861 tcatatttct gctttatcgt tggtttcacc ttttcaaatg cgatttcttt tgattcattc
     7921 caagaatcta aaatagaaac gtccaaagga ctttctactt ttgtatatga agaagaaact
     7981 gttttatctt tataaagcat cgccttcact ttgtcaaaac actttgcgcc aacacttgcc
     8041 aaatacaatc ttgaatatgt ctcatgacat aaaaacgaat ctggcaataa tggagcatat
     8101 aataaaatca acatatcccc ttcatgaacg tatgtttcaa tcaaatgata ttgttccaaa
     8161 cgatttcttg cgatgtcaaa ttgcgaagca ttcatattca ataaaagata tacatcttct
     8221 aattcaacca aattttgaat ggaacccaaa aattcatata gacaaattga gtttttcccc
     8281 attaaaggac catacaataa atttaaagat tgtctttgtt cgctagataa atgttctatg
     8341 catcgtactt caatcgccat ttaacttctc ctcttttaat gcatcctcaa tctgcatatg
     8401 caactgttct accgtactat tattacgtaa tacaatatca ctttttgcca ttttctcttc
     8461 tggattcatc tgtgatacaa gtcttgccaa agcttgttcc ctggtaaaat tacgatacgt
     8521 ttgtagacgt gataatgcca catctaaatc agccacaacg caccaaatag aatcaaaatg
     8581 ttcttgtgct gaaatctcaa ataaaattgg aatctccaca aagacagtta agctttcttg
     8641 ttcatggatc catttgtgca tttcatcaaa gattaaagga tgtaaaatcg cttctacttg
     8701 ttttcgatgc tcttcatttg aaaacatata ggaagctaga ctctccttaa taatttctcc
     8761 attcgaatcc aattcaacaa tgtgtaaatc atttaatttt tgataaccca aatttccctt
     8821 ttttaataaa tccgcattga cctgatcaca atctaaaaca ggatattttt ttcttagata
     8881 tcgagaaatt tcgctctttc ctgagcccat tgatcccgtg attccaatta ctttcttttt
     8941 tgacattttg gacataaata agtccccctt tgtgatacag ttaatttttt tattgtatga
     9001 tgacacactt tacattgcgc ctgaccatgt acttttaaat gaagttgaaa acgccccgtt
     9061 acacctaaac ttgatgtata ggaacgaatg gtcgttccac catagtgtgt ggctccttgc
     9121 aagatccgtc ttgtttctaa aacaattttt tcacaatctt tttttgataa cttgcaagca
     9181 cgacttcttg gatctaagcc tagcgcaaaa catatctcat cggcatagat atttccaata
     9241 cctgccataa tcgattgatc caaaagacaa gattttaaat ttcgttttaa tgtatgaata
     9301 caatgataga aatacattcc atttactctt ggatccatat aatcatatcc aacattttta
     9361 aaacaagcat acgaatgaat atcatcattt ttagaatata aatacattct tccaaacttt
     9421 cgagtatcat gatacgacat acagattcca ttatttaatt tgaatacgac atgaatatgt
     9481 ttgtcctcgc aaattttatc ataaagataa aacttgcctt ccattcttag atgaacaact
     9541 aaatcataat catctaagcc aaaaatcaaa tattttccaa gccgattaaa tgtacgaaag
     9601 gattgcccaa tcaatcgatg tttaaaagtt tctacttcta cattatcaac aatcttagga
     9661 taatatatca atacatcttc aattgaaata tttttaattt gtgtttccaa cgtacttaat
     9721 acggtttgta cttccggtgc ttccggcatt attttgcctc ataccaagac ttgccaacac
     9781 taatgcttgc ctttaatgga acctttaatt ccattgcact ttccattgtt tctttgacaa
     9841 gtttttgcat caagtccaac tcttcatctg gcaccaagaa aatcaattca tcatggatct
     9901 gaagaagcat ttttgagttt acgttggcat cctttaaagc ttgatccatt ttcaacattg
     9961 caatcttaat caagtctgct gcactcccct ggattggcgc attcatcgca gcacgcttac
    10021 caaattctcg tgtcataaaa ttcttgtcat tgatttccgg aatttctctt cttcgatgga
    10081 ataatgtttc tacatatcca ttttctttac agaaatcaat caattgattc atatactcat
    10141 gaatttttgg ataactttca aaatacatct tcataaactc acccgcctct ttgcgtgtga
    10201 tatttaattg tgatgataag ccaaattccg tttgaccata tacaattcca aagtttactg
    10261 tctttgcgat tcttcttttg tgttcattca cttcttctgg tgaacaatta aaaatcaacg
    10321 ttgccgtttt tgtatgaata tccaatcctt cattaaacgc tttaatcata tgatcttcat
    10381 ttgccatatg tgctaacatt ctcaattcaa tttgcgaata atccgctgac aataagtgat
    10441 aaccttcttg agcaacaaaa gctttacgaa tttcttttcc caactcatca cgaatactaa
    10501 tattttgaag atttggttca ctacttgaaa gtcttcctgt ttgtgtcatg gtttgattaa
    10561 aagaagtatg aatcttttga tctgaacgaa catgtttttt caaaccatca atatatgtgc
    10621 tagatacttt tgatatttta cgatattcca agatatcatc cacgattgga tgttttccac
    10681 gaagcttttc gagtacatca gcagaagtcg aacgcttctt accgcctgag cttaaattta
    10741 attcatcaaa taaaacattc gctaattgtt ttggagaatt gatgttaaaa atcatgccgg
    10801 catgagaata aatttgttga gccaaaatct ctagcttttc atttaataaa gccccatatt
    10861 catccaagaa gctttcttga atcgaaatcc cttcttgttc catagaaaat aatactcgtg
    10921 taagtggcat ttcaatttct ctatataaag aaagcacatt ttcttctttt aaagattgaa
    10981 ggatttcacc tttcttttca tataactgtt gtgtcaaact atgacaaagt gcttgttctc
    11041 tattcaaact aaaagcaggt tcttctttcg tctttttgga tacatcatga aaagattctg
    11101 gcaaagtaat atgaagagct tctagtacgg catcactatc tgttgcagta gaattcaata
    11161 agaatcctgc aatatgaaga tcatcattaa atgtattcca tttaaatcca tatcgatcta
    11221 atagatgcat catttctttg gtatcccatg tactaatttc ttttgtttct aatatttttt
    11281 taaagtttgt atcttccaat gcattttcta cggaaatata gtaaattacc ttatcttttg
    11341 gaatcataaa accatataat tgctgatcta agtatggttg ttgtgtacat actggtagta
    11401 ccattaaatc ctctacatca aacatttcaa aatgttccac ttcttttaat ggccattttt
    11461 cgttcttagc ttgctttgtt cgattcacta aagatcgcat ttcatatttt tcatagaatc
    11521 catttacatc ttcttgaatt cctgtaaact cacaatcatt taaactaaac ggaagttcca
    11581 tctttgtata aatggttgcc aattctaaag acataaacgc attgccttta tctttttcca
    11641 gcttttcctt taattttccc tttacctcat caatatgggc ataaacattt tctacagttg
    11701 aatattgatt caataaacgc aaagccgttt tttcaccaac accttgcaca cctggaatat
    11761 tgtcggctgt atctcccatt agtcctttca tatcaatgac ttggcttggt gtaattccat
    11821 atgtatcgaa tagattttgt tcgtccatca attccatttc acttaagcct tttttcatta
    11881 aaagaacatg tgtacttgga tcaatcaatt gaagtaagtc acgatcactt gttagaatgg
    11941 ttgtttgaat atcattacaa catttagcca tacttccaat aatatcatcc gcttcatagc
    12001 cttcttgttc atagcgtttg attccagctg catctaaata ttctcttaca attggaaatt
    12061 gtacgatcaa ttcctcatct aatggcttac gtgttccttt ataggctgca aattgtttgt
    12121 gtctaaaagt cggttttcct gcatcccaag caactagaat cttatcgggc tcaataatat
    12181 caattgcctt atttaacatc atcacaaagc cataaactgc attggttgga atcccattgg
    12241 atgttgtcat tctgtgtgta tataaagttg cataatatgc acgaaatagc atggagtttc
    12301 catctaataa taatagtttt ttcatctgaa tacctctttt cttctatttt aacacatatg
    12361 aaaagaagga aacgaatcca cgactcattt ccttcgaata ttttgcatgt gcaacttttc
    12421 cattcgttta aaacgaatat tttttatagt tgcatatcga ttgaataccg taataatata
    12481 gagcaataca aatatccaag ccccattaaa tatcaatgta agtgcttcaa cgtgaataaa
    12541 ccatttcaca aagctcattg taagatatcc aaataatttt gcccccaaaa aacaaattaa
    12601 atcatttata aacatcatta aaaagacaag ggcaaattgt acccagtgat cttgatccat
    12661 tgcattttta aatagtcccg atagccatcc aaaaaaacca aatagaacca ttttaacaag
    12721 ccaatccgaa gtaaagaaca atcctacaca aaggccgcat aggcatgcat ataaaagtcg
    12781 atttaagctt ttttcatgac tcacaacaag tatgcatcca caaaaacata aatgactgac
    12841 aaaagatagg gaagaatatg taaaatccac accgaataaa gatgcattca tttgatcaag
    12901 tattgctaaa attaataaga aaataaatga gcaacgttct ttatttatca tttactctcc
    12961 tcttttccaa tcacaagaca atagtcaaaa cttttcatat tcgatactgg cgaaacatag
    13021 accgttgaaa taatcgaatc atcgtttaaa gacacatctt ttacttctcc aattaagatt
    13081 ccacttggat aatttccacc cttaccactt gtagctactt tttgtccttt tgtgacaatc
    13141 gcatcattat caaataatga aacacgatag gccttctttt cagtatcata agactgtagt
    13201 acaccctcta cacttgaacc gtcttctaat gacatcttga tcgcaatatc attatttaaa
    13261 tgttgacttg tcaacaactg aactgtactc gtagaggttt gtacactatc cactaatcct
    13321 atagcgccat cgcttgttgc cacaagcata tttttttcga caccttgtct ttttcctgct
    13381 gaaatcgtta ccgtctgaag ccatgaatcg cctgaacgtt ctaatacaga tacactgatt
    13441 gtcttagcat ctccaagacc acctttcatt tccaacaagg cttcaagttg ttggttttta
    13501 tttctttcat cttcatatgc cgtttgatag tttttttgtt gagctaattg cttcgtcaaa
    13561 tattcattat cgtcataggc atgccataaa tttgaaaaat catttaccgc atttccaatc
    13621 gatgatagtg gatattgaat caaaccatat ttcaaatatg tccaagcact gtaaccaata
    13681 tttgtgatgg agttttgtct taagccaaac atagcacacg ctaatataca taaaacgaca
    13741 ataatcgtaa gtaatctttt ttgaattcga gtaaatttac gcataatatc ccccaaacta
    13801 acttatatta tagcgataaa ccctccttct ttcaatcgag atatttgtgc tctttaaatg
    13861 aataatattg atttttacat acaataatat gatccactaa acaaaccccc attgtcatcg
    13921 ctacatgaac cattttgtaa gttacttcaa aatctgcttt tgaaggcgta caatcccccg
    13981 aaggatgatt atgtgcaatc aatactgaat ttgcgttttg taaaaaagcc tccttaaata
    14041 cttcacgtgg atgtaccacg ctttcattta atgttccctt aaataaaagc gtggtacatc
    14101 cacgtgaagt atttaaggag gc
//
LOCUS       DBMDWL010000037        14050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1465_length_14050_cov_2.787798, whole genome shotgun sequence.
ACCESSION   DBMDWL010000037 DBMDWL010000000
VERSION     DBMDWL010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_14050_cov_2.787798"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            76..414
                     /locus_tag="ACZPDN_04010"
     CDS             76..414
                     /locus_tag="ACZPDN_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297820.1"
                     /translation="MKKRTMYILCLSAVVTVCTFKAFAASYHEPIQVEGPTINFDVES
                     EVQAQQYVDFKFKGLKNSQHIILVKGGGYLLNEDGSDVDKSDLQNGDRIITVKQAKEI
                     EKASYFEDED"
     gene            432..752
                     /locus_tag="ACZPDN_04015"
     CDS             432..752
                     /locus_tag="ACZPDN_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297821.1"
                     /translation="MKKIRLLIPLFIGLVAVTAYPAFARSSEEPVNVEEVNEENEDVG
                     YFPGCDDDEKVIPLGDGFLGGEAYLQNGDEEIYLNSETDSSAITVKEAKEILKSRQAE
                     SDNQ"
     gene            1058..1927
                     /locus_tag="ACZPDN_04020"
     CDS             1058..1927
                     /locus_tag="ACZPDN_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HIR1297822.1"
                     /translation="MKKLVQAFVSLIIATSTFGGVTNVAAKENTPAYKIEYQDADSMK
                     SVFKKELHKKYANVEFKKKTKNVSVYESKNYKVKVKDLDIDAIGKQTISIEGENKQTS
                     EKHSVEVNVKVQDTTAPEITCEDVLTVEQNEVFDINSHVSLNEEGTIQLTDNINTADV
                     GTFTTTIKAKDTAGNVSEKNITVHVEKSFYQRIADAALAQIGVYQDCTMLVTNSLAVV
                     GIHFHGAPTAYLSLGTLTNDPVPGDICVYQGHVALYVGNGQAVHGGWLGNQTVLTSVA
                     CGQPFIGYVHVNR"
     gene            complement(1999..3162)
                     /gene="fucO"
                     /locus_tag="ACZPDN_04025"
     CDS             complement(1999..3162)
                     /gene="fucO"
                     /locus_tag="ACZPDN_04025"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HIR1297823.1"
                     /translation="MANRFILNETSYHGAGAIKEVVTEIKARGLKKGLVVTDKDLIKF
                     GVATKVTDLLDEAGLPYEIYDEVKANPTVEQVKNGVQAAKNAGADYLIAIGGGSPQDT
                     CKGIGIILANPEFEDVVSLEGVAPTKNKSIPVIAIATTAGTAAETTINYVITDTEKKR
                     KFVCVDAHDIPVVAIIDPEMMSSMPKGLTASTGLDALTHAIEGYTTLAAWELADTLNL
                     KAIEIISRSLRAACANDPKGREDMALGQYMTGMAFSNVGLGVDHGMAHPLSAFYGIPH
                     GVACATLLPYVMEFNKDYTGEKFREIARVMGVEGVDEMSQEEYRQAAIDAVIQLSKDC
                     GIAQSLKEIGVKEEDLPTLTDSAMADVCTGGNPRPCTKELIFDLYKYAFNGRD"
     gene            3320..4255
                     /locus_tag="ACZPDN_04030"
     CDS             3320..4255
                     /locus_tag="ACZPDN_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1297824.1"
                     /translation="MENLLFSLNATIPIFLLMVVGYFLNKMNILEDALASKLNSFVFK
                     VSLPLLLFVDVSKSNFKDLWDTKFVVFCVVSTLISILLSILFSLRLKDKTIQGEFIQA
                     CYRSSAALLGLALTSNIYPDVGVAPLMLMSVVFVYNVVSVVILSFFKAEQSKVNYKKT
                     FVGILKNPLILGIVAGILVSLLPFQLPQVINKTISMIASTATPLGLMAMGATFDFDQA
                     KGELKPSLFCTFMKLIGFCALFLPIAALLGFRNQALVAILIMCGSSTTVSGFVMAKSM
                     GHKGVLTSSVVMLTTLLSAFSITFWLTILKSFGLI"
     gene            4314..4970
                     /locus_tag="ACZPDN_04035"
     CDS             4314..4970
                     /locus_tag="ACZPDN_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1297825.1"
                     /translation="MELFLESFPKIFISGIIMTLPLALVSFIIAMLLAIVCAMIQYAK
                     VPIAKNIVQFYIWIFRGTPMLVQLFIMYFGMQSIGVRISAITAAIIVFSLNTGAYCAE
                     TIRGSIESVPQGQMEAGYCVGMSYMQIMMHIILPQAFRSAFPSLFNNLISLVKDTSLA
                     ANITVTEMFMQTERIAGRTYQFMQLYIEVAFVYLIFCTVLTWLQAWGEKKLNYYQAGR
                     "
     gene            4974..5714
                     /locus_tag="ACZPDN_04040"
     CDS             4974..5714
                     /locus_tag="ACZPDN_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1297826.1"
                     /translation="MALLEIKDIHKSFGNLHVLKGIDLTVNKGDVIAILGPSGSGKTT
                     LLRCMNYLETADAGTLIFKDQSYDLSNTSKKEIADLRKHTGFVFQNYNLFANKTALQN
                     VTLGLTVARNVSKEEANVIGMQMLEKVGMQDRSNHYPSQLSGGQQQRVAIARALATSP
                     DIIYFDEPTSALDPELIQEVLSVMKTLANEGMTMVVVTHEMAFAKNVSSHVILMEEGK
                     IIEEGSSKEFFENPKQERTKEFLRKEEM"
     gene            5716..6498
                     /locus_tag="ACZPDN_04045"
     CDS             5716..6498
                     /locus_tag="ACZPDN_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1297827.1"
                     /translation="MKKLLAGLLSTAMVFTLVGCGSSKDHLATIQDKGEITIGLEGDW
                     QPFSYHDKNDKLVGVDVEVAKNIAKKLGVKANIVEGKWDGLLTGLSTGTYDLVINGVD
                     ITKEREKKFDFSTPYAYDHTVLVVRNDNTTITSFDDLKGKTTANSIGSTYMELGEDYG
                     ATVKGVDDLTKCMDLVKSGGVDATINAATSINDYLQTTKDTSFKIVDQSKESTPYAIP
                     MVKGDDNTSLKKAVNKALKELKEDGTLSKISIKYFGEDLTKE"
     gene            6625..7830
                     /locus_tag="ACZPDN_04050"
     CDS             6625..7830
                     /locus_tag="ACZPDN_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1297828.1"
                     /translation="MSELKNQISQFIDTNAKIYQDISLAIHAKPEVSDFEYFASQTLS
                     EQLKKEGFEIELPAAGHRTGFAATYKSNKPGPTVVFLAEYDALAGLGHGCGHNVFGAT
                     SSLAGAALKSIVDQIGGEVRVYGTPGEEGGQNGSAKGSFVKKGYLKDVDFALCVHPGS
                     GPEDGLSTRNYACAPVDIEFWGKPAHAAGCPQDGINALDAQILTYAAIGVLRQQLTDR
                     IRIHGVIVEGGTAPNVIPEYTKAKYYIRAADIDTLHELYEKVENIVKGSALQTGCTSS
                     MKLYQNLVENMVLTPSLDAIYEKYITELGNTVKHVEDVVMPGSSDVGNISQVVPTIQP
                     HISITDVQIAGHSQDMVNASCSQKAMDAIVKGAKALAFTALELFENPEELVKVKEDHA
                     WHVEHQKEN"
     gene            7928..8848
                     /locus_tag="ACZPDN_04055"
     CDS             7928..8848
                     /locus_tag="ACZPDN_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1297829.1"
                     /translation="MTLQQLLYVLTVSDEKTMNKAAEKLFVSQPTLTSAIQSLEKELN
                     IQIFNRTSHGVTVTQQGQEFLTYARQLYQQYELLKNRYEDVSLRRIKFRVSCQHYSFA
                     TKAFVDTVKKYGTSKYDFAISETKTMHVIEDVGNSLSEIGILYLSNYNRHFMMKQFDE
                     WNLEFHKLADCDAFVYLYKGHPLAKKKSITYEELLPYPNMTFDQGDNPSMYTAEEILV
                     ENEFPRKIQVNDRATMLNLMRGLNGYTLCSGIISKDLNGDDYVVVPYQANVENPNSMM
                     EIGYITRKYTVLSEIGNTYIQTMKDYFSNK"
     gene            9073..10119
                     /locus_tag="ACZPDN_04060"
     CDS             9073..10119
                     /locus_tag="ACZPDN_04060"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIR1297830.1"
                     /translation="MINIEHLDKTYHLKNADVHAVDDVSLHIESGQIYGVIGYSGAGK
                     STLVRCLNFLEIPDSGSIEIEGFGKVNANQGSLDISQKKLRELRRSIGMIFQHFNLLD
                     RSTVFDNVAYPLKYTGLSKKEIETRVDELLDLVDLADKKYVYPSQLSGGQKQRVAIAR
                     ALSNNPKVLLSDEATSALDPDATESILKLLKDLNKRLGITIILITHEMSVIKSIANRV
                     AVMENGKVVEEGDVYDIFANPKEEITKKFINSSSSLSNITKLIENKTIIPRDSKLVKL
                     TFTRDSVGSATISKISREYNVDVNIMLANVDVVNADALGGIIASIEGNKEDIQDAINY
                     LHASNVKVEVIHHA"
     gene            10112..10810
                     /locus_tag="ACZPDN_04065"
     CDS             10112..10810
                     /locus_tag="ACZPDN_04065"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIR1297831.1"
                     /translation="MPKFIADFFPNVANYWDIFLTALNATYQMFFIAGLITLLIGGAF
                     GILLIVTKTDGIKPNRMIHFILEVITNVFRAVPFIILLIFLIPFTRSIAGTSIGVKGA
                     IVPLVFGAVPFYTRQVETALADVNPGKIEAARSMGSSTLGIIFRVYLHEALPDLIRVT
                     TITAISLIGLTTSAGAVGAGGIGSFAINYGQNLHYQDVVNLCVILLLIIVSLIQLLGN
                     TLSSATTNKELFHK"
     gene            10828..11745
                     /locus_tag="ACZPDN_04070"
     CDS             10828..11745
                     /locus_tag="ACZPDN_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015159.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1297832.1"
                     /translation="MKTNKLFKTFLTAGLVATTLLTGCSSKSSEPVKIGIPSDATNGG
                     RGLLLLEKAGLIEVDDKAGWTPELKDVTKYKYNIEIVPTQANTLVSTLDDFGAATING
                     TYAIPAGLSPKKDGLITEVQEVGSDNPFINVIVARTADKDNVDYQKVVKAYQSQLVAE
                     YILEKNKGASVPAFEYDEDYTVDKNFVSDIEGYQSSSDGKKVIKIGTCGSADTFRAVQ
                     KVLDDENSGIYLDVVVFDAYNLPNEALNNGEIDLNSFQHKAYLENECKAQGYDLTAIG
                     DTSSAPLTLYSKKVDSLKALKELAGKKED"
     gene            complement(11772..12077)
                     /locus_tag="ACZPDN_04075"
     CDS             complement(11772..12077)
                     /locus_tag="ACZPDN_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HIR1297833.1"
                     /translation="MEKDWKLFRKKLGGWQESYMDKLVEEYKSILNSDALSSEKYWAL
                     RKKIYEDYKSPGVLARDVSRSNMLWILLGLLRDGAITMDDLDAFSDELKEDIKQFIK"
     gene            complement(12087..12461)
                     /locus_tag="ACZPDN_04080"
     CDS             complement(12087..12461)
                     /locus_tag="ACZPDN_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297834.1"
                     /translation="METKLWSALIGLSKTVDSNPKTENTDTIIINCLQHLRNHTVTQD
                     LIDLVHKEKDKISPSCKTCTHPCGNTSDYDMSLINDKKKELMHQILNLRDIHYIYRGL
                     CYLGFDIDDSYIDELIKECKDK"
     gene            complement(12451..13065)
                     /locus_tag="ACZPDN_04085"
     CDS             complement(12451..13065)
                     /locus_tag="ACZPDN_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1297835.1"
                     /translation="MKLLNIQKEIPDILLDIRYCTTYNFIGEIIDGYIDPVALGTKEL
                     VKQLKVINSKARQMGYRLKIWDAYRPQSALDHFMTWKDTEDDKMKSIFYPKYSKKYLF
                     ENDFIIEKSAHTRGSTVDLTLVDEKTGKELDMGSYFDYFGDISSPLHKDGLTTIQIQN
                     RMTLRNLMLDNGFIPLESEWWHYTLKDEPYPDTYFNVEVKSDGN"
     gene            13145..13747
                     /locus_tag="ACZPDN_04090"
     CDS             13145..13747
                     /locus_tag="ACZPDN_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297836.1"
                     /translation="MKFSDIDFSAISRMMDNMSDEEKNKLNDMAQNMMNNMKQNEEPE
                     EETDFYEALNINEEDYAEFPGSVLDQIEAGSDLEVYYEDVKDADFSASALFYAKATLN
                     MLRKYIYPVFKNFFDGFNNPSTTTIYSYLYPLMNEDNIHKLFDEEFGTPEGWIELKNA
                     LQQIYVILNRAEYDFVSYEDLQLLKDILFNQEILLKIKNL"
     gene            13744..>14050
                     /locus_tag="ACZPDN_04095"
     CDS             13744..>14050
                     /locus_tag="ACZPDN_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013003.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR1297837.1"
                     /translation="MKKLKINTSLEPMDEWTYESYVSDYIFENVCIEQNLDNVTIDGC
                     KFSKCQFKDCSFSFMECMDIIFDHCEFENVHFNQCSLSRVHFISCRISGMEFSQSLMQ
                     "
BASE COUNT         4717 a         2027 c         2722 g         4584 t
ORIGIN      
        1 cttgcaaaaa tacgcaatcg gaatataatg aagataggaa aacgttttcg caaaaaaaga
       61 aggaggaaaa gacttatgaa aaagagaacg atgtatattt tgtgtttatc tgctgttgta
      121 acagtttgta cctttaaagc atttgcagca agttatcatg agcctatcca agtagaaggt
      181 cctactataa attttgatgt tgaatcagag gtacaggctc aacaatatgt ggattttaaa
      241 tttaaaggat tgaagaatag tcagcatatt attctagtaa aaggtggtgg ctatttactg
      301 aatgaagatg gctcagatgt ggataaatct gatttacaga atggggatcg aattatcaca
      361 gtgaagcagg caaaagaaat tgaaaaagca tcatattttg aggatgaaga ttaatggatg
      421 gaggtgaaac aatgaaaaaa attcgactat tgattccttt gtttattggt ttagttgctg
      481 ttacagctta tccagctttt gcaagatcga gtgaagaacc agtcaatgtt gaagaagtga
      541 atgaagaaaa tgaggatgta ggctattttc ctggatgtga tgatgatgaa aaagtgattc
      601 cattaggaga tggctttcta ggtggagaag cttacttaca aaacggggat gaagagatct
      661 acttgaatag tgaaacggat tcgagtgcaa tcactgttaa agaagcaaag gaaatcttaa
      721 aaagcagaca ggcagaatct gacaatcaat aatttgtact agcattataa attttggtgg
      781 agttaggtgg attttatcta caataattta aagtgcggta taagtttcaa aacgtattgt
      841 agctcgtatt ggacttggac aacattaact tgttcgggtc ctttttgtag ttcttttttc
      901 tactaaaaag tgcaaaaaaa tgggacagac ttgttaataa tggtaaatta aaacaaatta
      961 atatgttaaa ttgtgggaaa tacgggtatt cttttgacac tttggactaa atagtgtagc
     1021 ctctttaatt gtgtgagcaa ggaggaggcc cataactatg aaaaaattag tccaagcatt
     1081 cgtaagtttg attattgcta catctacatt tggaggcgtt acaaatgttg cagcaaaaga
     1141 aaacacacca gcttataaaa tcgaatatca agatgcagat tccatgaaat ctgtatttaa
     1201 aaaagaatta cataaaaaat atgcgaatgt agaatttaag aagaaaacaa aaaatgtttc
     1261 tgtatatgaa tcaaagaatt ataaggtaaa agttaaagat ttagatattg atgcaattgg
     1321 aaaacaaact atttctattg agggagaaaa taaacaaaca agtgaaaaac atagtgttga
     1381 agtaaatgta aaagtacaag atactacagc tcctgaaatt acttgtgaag atgttcttac
     1441 agttgaacaa aatgaagttt tcgatatcaa tagccatgtt tctttaaatg aagaaggaac
     1501 gattcaatta acggacaata tcaatacggc tgatgtagga acattcacaa caacgattaa
     1561 agccaaagat acagccggta atgtaagtga aaaaaatatt acagttcatg ttgaaaagag
     1621 tttttatcaa agaattgcgg atgctgcatt agctcagatt ggtgtatatc aagattgtac
     1681 aatgttagtc acaaattctt tagctgtcgt tggaattcat ttccacgggg cacccacagc
     1741 ttatttaagt ttaggtacat tgacaaatga tccagttcca ggtgatattt gtgtatatca
     1801 aggacatgtt gcattatatg ttggtaatgg tcaagctgtt catggtggat ggctaggtaa
     1861 ccaaactgtt ttaacaagtg tagcatgtgg gcaaccattt attggctatg ttcatgtaaa
     1921 tcgttaagat gagatgattc tcatcttttt tttcttgcac aaaaaaagaa aggcaaacat
     1981 ttgcctttca gcatataatt agtctcttcc gttaaatgcg tatttatata agtcgaagat
     2041 taattctttt gtacatggac gagggttacc acctgtacaa acgtctgcca ttgctgaatc
     2101 cgttaatgtt ggtaagtctt cttccttaac accgatttct tttaagcttt gagcaattcc
     2161 gcaatcttta cttaattgaa taacggcatc gatagctgct tgacgatatt cttcttgaga
     2221 catttcatca acaccttcaa ctcccattac acgagcgatt tctctaaatt tttcacctgt
     2281 gtaatctttg ttgaattcca taacatatgg aagtaatgta gcacaagcta caccatgagg
     2341 gattccatag aatgcgctta atgggtgagc cataccatgg tcaacaccta aaccaacgtt
     2401 agagaatgcc atacctgtca tatattgtcc taaagccatg tcttcacgtc cttttggatc
     2461 gttagcacat gctgcacgta agcttcttga aatgatttca atagctttta agtttaatgt
     2521 atcagcaagt tcccaagccg ctaaagttgt atatccttca atggcgtgag ttaatgcgtc
     2581 aagccccgta cttgcggtta aaccttttgg catacttgac atcatttctg gatcgataat
     2641 cgcaacaaca ggaatatcgt gagcatctac acaaacaaat tttcttttct tttcagtatc
     2701 tgtaattaca tagttgattg tagtttcggc tgctgtacct gctgtagtag cgattgcgat
     2761 aactggaatt gatttgtttt tagttggagc aacaccttcc aagctaacaa catcttcaaa
     2821 ttctgggttt gccaagataa ttccaatacc tttacaagta tcttgtgggc ttccaccacc
     2881 aatagcgatt aaatagtcag cacctgcatt tttggcagct tgtacaccat ttttaacttg
     2941 ttcaacagtt gggtttgcct taacttcatc ataaatttca taaggaagtc cagcttcatc
     3001 caataagtct gtaactttag tagctacacc aaatttgatc aagtctttat ctgtaacaac
     3061 taaacctttc tttaatccac gtgctttaat ttctgtaacc acttctttga tggctccagc
     3121 accatgataa cttgtttcat ttaaaataaa acgatttgcc ataagtatta tttcctcctt
     3181 gtatacttgt taatttcatt ttacgagtta aaccgcttac atacaagtta cagattgata
     3241 ataatgtcac atataagtgt tttagtgcat attatctaat atttgtgcta aaattttaag
     3301 aaagttttgg agggattttg tggaaaattt attatttagc ttaaatgcaa cgattcccat
     3361 ctttctattg atggttgtcg gttatttttt aaataaaatg aatattttag aagatgcttt
     3421 ggcatccaaa ctaaattcat ttgtctttaa agtgtcattg ccactattgt tgtttgtcga
     3481 tgtgtctaaa tctaatttta aagatctttg ggatacaaag tttgtcgtat tttgtgttgt
     3541 atctactcta attagtattc tattgtctat attattttca ttacgtttaa aagataaaac
     3601 gattcaaggt gaatttattc aagcttgtta cagaagtagt gctgccttat taggtcttgc
     3661 attaacatcg aatatttatc cagacgtagg tgttgcccca ttgatgttga tgagtgttgt
     3721 atttgtgtat aacgtagtat ctgttgtgat tctatctttc tttaaggctg aacaatcaaa
     3781 ggttaactat aagaagacgt ttgttggaat tttaaagaat cctctgattc taggtattgt
     3841 tgcaggtata cttgttagtt tattaccatt tcaattgcct caagttatca ataaaacgat
     3901 ttcaatgatc gcaagtacgg caacaccact aggcttaatg gcgatggggg ccacttttga
     3961 ttttgatcag gcaaaaggag aattaaaacc atctttattc tgtacattta tgaagttaat
     4021 tggtttttgt gctctctttt taccaattgc tgctctattg ggatttagaa atcaagcttt
     4081 agttgccatt ttgattatgt gcggttcaag tacaaccgta tctggttttg tgatggctaa
     4141 aagtatgggg cataaaggtg tattaacatc aagtgttgtg atgttaacaa cattgcttag
     4201 tgcttttagt attacattct ggttaacgat attaaaatcg tttggattga tttagttgac
     4261 ctaaagtttg ctttagggtg taaactaata aaaagatttt agggagggat agaatggaat
     4321 tgtttttaga atcattccca aaaatattta tttcgggaat tataatgaca ttaccacttg
     4381 cactagtatc atttattatc gcaatgcttt tagctattgt atgtgccatg attcaatatg
     4441 cgaaagtacc aatcgcaaag aatattgttc aattctatat atggatattt agaggaacgc
     4501 ctatgcttgt acaattgttt attatgtatt ttggtatgca gtcaattggc gtaagaatat
     4561 cggctattac tgccgctatt atcgtattct cattaaatac gggcgcttat tgtgctgaaa
     4621 cgattcgtgg ctcgattgaa agtgtaccac aaggacaaat ggaagccgga tactgtgtag
     4681 gaatgagcta tatgcagatt atgatgcata ttattttacc acaagctttt cgaagtgctt
     4741 ttccatcttt gttcaataac ttgatttcac ttgtaaaaga tacatcttta gcagcgaata
     4801 ttactgttac agaaatgttt atgcagacag aaagaattgc cggaagaacg tatcagttta
     4861 tgcaattata tatagaagtc gcattcgtct atttgatttt ctgtacagtt ttaacttggt
     4921 tgcaagcttg gggtgaaaag aagctgaatt attatcaggc agggagatag agaatggctt
     4981 tattagaaat taaagatatt cataaatcat ttggaaattt acatgtttta aaaggtattg
     5041 atcttacagt caataaagga gatgtcattg cgatattagg tccttcggga tcaggtaaga
     5101 caactttact tcgttgtatg aattatttag agacagcgga tgcaggaacg ctgattttta
     5161 aagatcaatc ttatgattta tcaaatacat caaagaagga aattgcagat cttagaaagc
     5221 atacgggatt tgtatttcaa aattacaatt tatttgcgaa taaaacagcc ctacaaaatg
     5281 taacattagg tcttactgta gctagaaatg tttccaaaga agaggctaat gtgattggta
     5341 tgcagatgct tgaaaaagta ggaatgcaag atcgttccaa tcattatcct tcacaattat
     5401 cgggtggaca acaacagcgt gtcgcaatcg caagagcatt agctacaagc cctgacatta
     5461 tttattttga tgaaccaact tcagcactgg atccagaatt gattcaagaa gtattaagtg
     5521 taatgaaaac actggctaat gaaggaatga ctatggttgt ggtcacgcat gagatggctt
     5581 ttgcgaaaaa tgtttctagt catgtaattt tgatggaaga aggaaaaata attgaagagg
     5641 gaagttcaaa ggaattcttt gaaaatccga aacaagaacg aacaaaagaa tttttaagga
     5701 aagaggaaat gtaagatgaa aaaattatta gcaggtttat taagtacggc tatggttttt
     5761 actttagtag gatgtggatc tagtaaggat catttagcta cgattcaaga taaaggtgaa
     5821 atcacaattg gtttagaagg cgattggcaa ccattttctt atcatgataa gaatgataaa
     5881 ttggtgggtg ttgatgtgga agttgctaag aatattgcca agaaattagg tgtaaaagcc
     5941 aatattgtgg aaggtaaatg ggatggatta ttaactggac tatctactgg tacatatgat
     6001 ttagtcatta atggtgtcga tatcacaaag gaaagagaaa agaagtttga tttttctaca
     6061 ccttatgcat atgaccatac agtcttagtt gttcgtaatg acaatacgac cattacttcg
     6121 tttgatgatt taaaaggaaa gacaacggcc aattcgattg gatctaccta tatggaattg
     6181 ggtgaagatt atggggcaac cgtaaaaggt gttgatgatt taacgaaatg tatggatctt
     6241 gtaaaaagtg gtggtgtgga tgcgacaatc aatgcagcta cttcgatcaa tgattatctt
     6301 caaacgacaa aggatacttc atttaaaatt gtcgatcaat ccaaagaatc tacaccctat
     6361 gcgattccaa tggtaaaagg ggatgataat acctctttaa agaaagctgt aaataaggct
     6421 ttaaaagaac tcaaagaaga tggaacttta tctaagattt ctatcaagta ttttggtgag
     6481 gatctaacga aagaataagc ggctatgccg tttttctttt tacttaaata taaaaataat
     6541 ttatagccag atataaataa atagaatttg aatatagcac ttgttctttg tacaatagat
     6601 tcaactttta ggaggaacta ttttatgtca gaattaaaga atcaaatttc tcaatttata
     6661 gatactaacg caaaaatata tcaagatatt tctttggcaa tccatgccaa gccagaagtc
     6721 agtgactttg aatattttgc ttcacaaaca ttgagtgaac aattaaaaaa agaaggattt
     6781 gagattgaat taccagcggc aggacatcgt acaggctttg cggcaaccta caaaagcaat
     6841 aaaccaggtc ctacggttgt ttttcttgca gaatatgatg cgcttgcagg tttaggccat
     6901 ggttgtggtc ataatgtatt tggcgctact tcttcacttg ccggtgctgc tttaaaaagt
     6961 atagtggatc aaattggtgg ggaagtacgt gtatatggaa cacctggaga agagggtgga
     7021 caaaatggaa gtgccaaggg gtcttttgtg aagaagggct atttaaaaga tgtggatttt
     7081 gccttatgtg ttcatccagg ctcaggtcct gaagatggat taagtacgcg taattatgca
     7141 tgtgcacctg ttgatatcga attttggggt aaacccgctc atgcagcagg ctgccctcaa
     7201 gatggaatca atgcgttgga tgcgcaaatc ttaacatatg cagccattgg tgttttacgt
     7261 caacaactaa ccgatcgcat tcgtattcat ggtgtgattg ttgagggagg aacagcgcct
     7321 aatgtcattc cagaatacac aaaggctaaa tattatattc gtgctgctga tattgataca
     7381 ttacatgagt tatacgaaaa agtagagaat attgtgaagg gttctgcttt acagacgggt
     7441 tgtacatcga gtatgaagtt gtatcaaaac cttgtagaaa atatggtgtt aacaccttct
     7501 ttagatgcga tttatgaaaa atatattaca gaacttggca atactgtaaa gcatgtagaa
     7561 gatgtggtca tgcctggaag tagtgatgta ggtaatatta gtcaggttgt acctacgatt
     7621 caaccgcata ttagtattac agatgtacag attgcaggac atagtcaaga tatggtcaat
     7681 gcttcatgca gtcaaaaggc aatggatgcg attgtcaaag gggcaaaagc attggctttt
     7741 acggcacttg aattatttga aaatccagaa gaacttgtga aagtgaaaga ggatcatgct
     7801 tggcatgttg aacatcaaaa ggaaaattaa tctttttata tttagagagc ttgtcaagct
     7861 cttttatttt ggaatcttga aaatcaacaa atattcatta aaataatatg aataaaggag
     7921 actgaaaatg acgcttcaac aattacttta tgtattaacg gtatctgatg agaaaactat
     7981 gaataaggcg gctgaaaaac tttttgtgtc acaaccaact ttaacgtctg ccattcaatc
     8041 gcttgaaaaa gaactaaata ttcaaatctt taatcgtaca agtcatggag tcactgtgac
     8101 gcaacaagga caagagtttt taacatacgc aaggcagctc tatcaacaat atgaattatt
     8161 gaagaatcga tatgaagatg tgagtttaag aagaattaag tttcgcgtat cttgtcaaca
     8221 ctattcgttt gcgaccaaag catttgtgga tacagtaaag aagtatggga cttctaaata
     8281 tgattttgcg atcagtgaaa caaagacaat gcatgtcatt gaagatgtgg gtaattcttt
     8341 aagtgagata ggaatcttat atttatcgaa ctacaatcgt cattttatga tgaaacaatt
     8401 tgatgaatgg aatttagaat ttcataaatt agccgattgc gatgcctttg tgtatttgta
     8461 taagggacat cctcttgcta agaaaaaatc aattacgtat gaagaattat taccatatcc
     8521 taatatgaca tttgatcaag gggataatcc ttcgatgtat acggcagagg agatattggt
     8581 tgaaaatgaa tttccaagaa agattcaagt gaatgatcgt gctacgatgc tgaatttaat
     8641 gagaggactg aatggatata ctttatgttc tgggatcatt agtaaagatt tgaatgggga
     8701 tgactatgta gttgttccgt accaggctaa tgtagaaaat ccaaattcca tgatggaaat
     8761 aggatatatc actcgaaaat atacggtttt aagtgagatt gggaatacgt atattcaaac
     8821 aatgaaagat tattttagta ataaataaaa tttattactg cttcaaaaat ttaacaatta
     8881 gacaatagct aaaccttctt gtagaataca aacatgcaag gaggaaacga aaatgaaaac
     8941 aatgatgatg tcaatgatgc aaatgtatat gttaaaaaat gtgacccggt tttcaattcg
     9001 tttgtcgctt atttaagtgt gcgattgccg gggaaatacc tcggcttttt ttgttagatt
     9061 ggagtgttat aaatgattaa tatagagcat ttagataaaa cttatcattt aaagaatgca
     9121 gatgtacatg ccgtagatga tgtttcattg catattgaaa gtggacaaat ctatggagtg
     9181 attggatatt ctggtgcagg taaaagtact ttggtgcgat gtttgaattt tttagaaatt
     9241 ccggattctg gaagtattga aattgaaggg tttggaaagg taaacgcaaa tcaaggaagt
     9301 ttagatataa gtcaaaagaa attaagagag ttaagacgat caatcggaat gatctttcaa
     9361 cattttaact tattagatcg aagtacagta tttgataatg tggcctaccc attaaaatat
     9421 acaggtttat caaaaaaaga aattgaaact cgtgtcgatg aattattaga tcttgtagat
     9481 ttggcagata aaaaatatgt ctatccatca caattatcgg gtggacaaaa acagcgtgta
     9541 gccatcgcaa gagccttatc caacaatcca aaggtgttac ttagtgatga agcaacttct
     9601 gctctagatc cagatgcgac agaatcgatt ttaaaattat tgaaggattt aaataaaaga
     9661 ttaggaataa caattatctt gattacacat gaaatgagtg tgatcaaatc tatcgcaaat
     9721 cgtgtggcag ttatggaaaa tggtaaggtt gttgaagaag gagatgtata tgatatcttt
     9781 gccaacccaa aagaagaaat cacaaagaaa tttattaatt cctcatcttc gttatcaaat
     9841 ataacaaagt tgattgagaa caagacaatt attccaagag atagtaaatt agttaaactt
     9901 acattcacta gagatagtgt tggtagtgca accatttcaa aaatatctcg tgaatacaat
     9961 gtggatgtaa atatcatgtt agctaatgtg gatgttgtca atgcggatgc cttaggaggt
    10021 atcatagctt ctattgaagg aaacaaagaa gatatacaag atgcgatcaa ttatttgcat
    10081 gcatcaaatg taaaagtgga ggtgattcat catgcctaaa tttatagcgg atttctttcc
    10141 aaacgttgcc aattactggg atatattttt aacagcacta aacgcaactt atcaaatgtt
    10201 tttcatcgca ggacttatca cattattgat aggaggtgct tttggcattt tgttgattgt
    10261 aacaaagaca gatggtatca aaccaaatcg aatgattcac tttattttag aagtcattac
    10321 gaacgtattt agagccgtac catttatcat tttattgatt ttcttgattc cattcacacg
    10381 aagtattgca ggaacttcca ttggcgttaa aggagcaatc gttccattag tatttggagc
    10441 ggttccattc tatacaagac aagttgaaac ggcattagca gatgtaaatc caggaaagat
    10501 agaggccgca agaagcatgg gaagttcaac acttggaatt atctttcgtg tttatctaca
    10561 tgaggcgtta ccggatttga ttcgtgtaac aaccattaca gctatttcct tgattggact
    10621 tacaacgagt gcaggtgcag ttggagctgg tggaattggt tcgttcgcaa tcaattatgg
    10681 acaaaactta cattatcaag atgttgtgaa tttatgtgtt atcttactac tgattattgt
    10741 ttcgttgatt caattgttgg gaaatacatt atctagtgca accacaaata aagaattatt
    10801 tcataaataa aaaaggagaa aaggattatg aagacaaaca aattatttaa aacattttta
    10861 acagccggct tagtagctac aacattactt acaggatgtt catcaaaatc aagtgaacct
    10921 gtaaaaattg gtattcctag tgatgccaca aatggtggaa gaggtttatt gcttttagaa
    10981 aaagctggct tgattgaagt ggacgacaag gctggatgga cgccagaatt gaaggatgtt
    11041 acgaagtata aatacaatat tgaaattgtt cctacacaag ccaatacatt agtttcgact
    11101 ttggatgatt ttggagcagc cacaatcaat ggaacatatg caattccagc tggattaagc
    11161 cctaaaaaag atggcttgat tacagaagta caagaagtag gaagtgataa tccatttatt
    11221 aatgtgatcg ttgcacgtac ggctgataaa gataatgtgg attatcaaaa agtagtaaag
    11281 gcataccaaa gtcaattggt ggctgaatat attttagaaa agaataaagg tgcaagtgtt
    11341 cctgcatttg aatatgatga agattataca gtggataaaa actttgtgag tgatattgaa
    11401 ggctatcaat caagttcaga tggaaagaaa gtgattaaga ttggtacatg tggttctgcc
    11461 gatacattta gagcagtcca aaaagtcttg gacgatgaaa attctgggat ttatttggat
    11521 gtagttgtat ttgatgcgta taacttacca aatgaagctt taaataatgg cgaaattgat
    11581 ttgaactcat tccaacacaa agcgtattta gaaaatgaat gtaaagcaca aggatatgat
    11641 ttaacagcga ttggggatac tagttctgct ccattgactt tatattcaaa gaaagtggat
    11701 tctttaaaag cattaaaaga attagctggt aaaaaagaag actaaatgaa aaagccgatt
    11761 tttcggcttt tttactttat aaattgttta atatcttcct ttagctcatc actaaacgca
    11821 tctagatcat ccatagtgat tgctccatcg cgcaataatc ctaacaagat ccaaagcata
    11881 tttgaacgag atacatctct tgccaaaaca ccaggacttt tataatcctc ataaattttc
    11941 tttcgtagag cccagtattt ttcagaagat aatgcatctg aatttaaaat ggatttgtat
    12001 tcctcaacga gcttatccat ataggattct tgccagccac ctagtttctt tctaaataat
    12061 ttccaatctt tttccatgtt gacctcctat ttgtctttac attcttttat taattcatca
    12121 atataagaat catcaatgtc aaaacctaag taacataaac cacgatagat ataatgaata
    12181 tcccttaaat tcagaatttg atgcatcaat tcctttttct tgtcattgat caaagacatg
    12241 tcataatcac ttgtattgcc acaaggatgt gtgcacgttt tacaacttgg cgagatcttg
    12301 tctttttctt tgtgtactaa atcaattaaa tcttgtgtaa ctgtatgatt tcttagatgt
    12361 tgaagacaat ttatgataat tgtgtctgtg ttttctgttt ttggatttga atccacagtt
    12421 tttgaaagtc caattaaagc ggaccataat ttagtttcca tcagatttta cctccacatt
    12481 aaaataagta tctggatagg gttcatcctt taaagtatag tgccaccact cggattctaa
    12541 agggataaat ccattatcta acattaaatt tcttaatgtc attcgatttt gaatctgtat
    12601 ggttgttaat ccatccttgt gtaaaggact agaaatatcg ccaaaataat caaaataaga
    12661 tcccatatct aattcttttc ctgttttttc atcaactaag gttaaatcaa cagtactacc
    12721 cctggtgtgt gctgattttt cgatgataaa gtcattctca aaaagatatt ttttggaata
    12781 ttttggatag aaaatggatt tcattttatc atcctcagta tctttccaag tcatgaaatg
    12841 atcaagggca ctttgaggtc tgtaggcatc ccatatcttt aatcgatatc ccatttgtct
    12901 tgcctttgaa ttgattactt ttaattgctt caccaattct tttgttccta acgctaccgg
    12961 atctatatag ccatcaatga tttcaccaat gaaattgtag gttgtgcaat agcgaatatc
    13021 caaaagaata tcggggattt ctttttgtat atttaataat ttcatgtata tattctaata
    13081 tatttgttta tgttaatcca cttatgtttt gtgtataata cttgttagaa atgaggtaat
    13141 aaacatgaaa tttagtgata ttgatttttc agctatttca agaatgatgg acaatatgag
    13201 tgatgaagaa aagaataagt taaatgatat ggcccaaaat atgatgaata atatgaaaca
    13261 aaatgaagag cctgaagaag aaacggactt ttatgaagca ttaaatatca atgaagaaga
    13321 ttatgcagaa tttccaggaa gtgtcttaga tcaaattgaa gcgggtagtg atttggaagt
    13381 gtattatgaa gatgtaaagg atgcggattt tagtgccagt gccttatttt atgcgaaagc
    13441 cactttaaat atgttgcgaa aatatatcta tccagtattt aaaaattttt ttgatggatt
    13501 taataatcct tcaaccacaa ccatctattc ctatttgtat cctttgatga atgaggataa
    13561 cattcataag ttgtttgatg aagagtttgg tacaccagaa ggatggatcg aacttaaaaa
    13621 tgcgcttcaa caaatttatg ttattttaaa tcgtgcagag tatgattttg tgagttatga
    13681 agatttacaa ttattaaaag atatcttgtt taatcaagaa atattgttga aaattaagaa
    13741 tttatgaaaa agctaaaaat caatacgagt cttgaaccaa tggatgaatg gacatatgaa
    13801 tcgtatgtga gtgattatat atttgagaat gtttgtattg aacaaaactt agataatgtc
    13861 acaatcgatg ggtgtaaatt tagtaagtgt caatttaaag attgtagctt tagctttatg
    13921 gaatgtatgg atattatttt tgatcattgt gaatttgaaa atgtacattt taatcaatgt
    13981 agtttaagcc gagttcattt catttcttgc cgaatcagtg gaatggagtt ttcacaaagc
    14041 ttaatgcaag
//
LOCUS       DBMDWL010000038        14016 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1469_length_14016_cov_3.355731, whole genome shotgun sequence.
ACCESSION   DBMDWL010000038 DBMDWL010000000
VERSION     DBMDWL010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 14016)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14016)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14016
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1469_length_14016_cov_3.355731"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            271..1515
                     /gene="pepT"
                     /locus_tag="ACZPDN_04100"
     CDS             271..1515
                     /gene="pepT"
                     /locus_tag="ACZPDN_04100"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1297838.1"
                     /translation="MTEFYEQVKERLMRYARINTESDPHSTSVPTTSCQHDLASIIYE
                     ELQKIGATNVYYDKDTCVVYGSLEANVKTGRAIGFVTHMDTAPDASGKDVKPWVLENY
                     DGKDIVLNAEKDIVMSPSVFPNLKQYIGQDLILTDGTTLLGGDDKASIAAVMTLLEYL
                     VKHPEIKHNFISVAFTPDEEVSGLAKDLDLERFKSPIAYTLDGDHLGYYMDETFNASL
                     STIEIHGISVHTATAKGIMKNAVDIGNAFLNKLPALEKPQYTQGREGFYHVVSFNGDC
                     EYAKIEINVRDHDSLQFDIRNNTLKMIVDMLNEEYGQGTVNITQRIQYRNLKEVIDQV
                     PFMIPYLKQAIESVGLTPQTEPFRGGTDGSALSHRGLPCPNLSAGYENAHGRFEYVPI
                     QSMEKNVEILLNLIDIYAKCDC"
     gene            1573..1658
                     /locus_tag="ACZPDN_04105"
     tRNA            1573..1658
                     /locus_tag="ACZPDN_04105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1606..1608,aa:Leu,seq:cag)
     gene            1669..1744
                     /locus_tag="ACZPDN_04110"
     tRNA            1669..1744
                     /locus_tag="ACZPDN_04110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Thr,seq:tgt)
     gene            1840..2559
                     /locus_tag="ACZPDN_04115"
     CDS             1840..2559
                     /locus_tag="ACZPDN_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HIR1297839.1"
                     /translation="MKEVFEVGLDIIKKIQETQSEKLEQTGHLVAKAFMDGHKFFVSG
                     SGHSHTMAEEMYARAGGLAFVVPILTSELTMTEHPTKSSHIERLSGYAKILAELYGIS
                     CGDVVLIASNSGRNAYPIELALEAKNRGAHVVAITSMKHTTAQSSRHSCGKNLYQIAD
                     IVIDNCGEVGDCALSMDGLDAKLCPTSSMANSFIAQCINVACAKYLIEHNVEVPIFSS
                     LNCDGTEERNDKLFKKYTRMY"
     gene            2580..3686
                     /locus_tag="ACZPDN_04120"
     CDS             2580..3686
                     /locus_tag="ACZPDN_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1297840.1"
                     /translation="MKKRNLWIYIFGITIIIIGLIKLDTLISMVSLLWNACRVLVFGA
                     MMAFVINLVMAKIEKRLDFMKKGKRATSLLLSLILIFLFVYFLITLVVPSLSEAIGVI
                     VQVLPKYFENAQKFLVDLFENNPQLADSIGNLDIDWKNLIQSGINVLSTGVTNVVGTT
                     FNVVTVIVNSVFNFVLMFIFAIYVLLEKERFVQLYHRLTKLYLSETMKRRMDKCLHII
                     NQTFSAFIGGQCIEATILGTLCTLGMIILRMPYPAMIGTLVGVINIIPMIGAYIGGAI
                     GMFMVFTVSPIMSLWFLLYLCILQQIESNVIYPHVVGNSVGLPGIYVMMTVVIFGSLA
                     GIPGMFLGIPTMASVYKIGVLYLSRKERLKEMNG"
     gene            3748..5127
                     /gene="purF"
                     /locus_tag="ACZPDN_04125"
     CDS             3748..5127
                     /gene="purF"
                     /locus_tag="ACZPDN_04125"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1297841.1"
                     /translation="MFDEIHEECGVFGAYRVDNAASITYYGLHSLQHRGQEASGIAVS
                     DGENITLQKGKGLTVDVFQKEKLDSMVGRLAVGHVRYSTAGGQENENIQPIVSKGHNG
                     SLAIVHNGQIVNEKELRIELENEGAIFQGTSDSEIILHLIQKQKGTLKERVMKTANRI
                     EGAFSFLVMNEDTIYAVRDRHGLRPLSYAKSKDGYVISSETCAFEVMGIYESVDLKPG
                     EIVEFHKGIVKHEFYSTDTDHHMCAMEYIYFARPDSVVEGINVHAFRKTTGSILARED
                     KDLKADIVIGVPDSSLSAAIGYAEEAGIPFETGLVKNRYVGRTFIQPTQAMRDRSVRL
                     KLSPVSSVVKGKSIVMIDDSIVRGTTSRRIVQLLKDAGATQVHVRIASPVITSPCFYG
                     VDTSTKDQLIGARMSVEELREYICADSLRFMTEEEMKEATHGVGLCLACFNGEYCTKL
                     FSYQEELDK"
     gene            5129..6013
                     /locus_tag="ACZPDN_04130"
     CDS             5129..6013
                     /locus_tag="ACZPDN_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1297842.1"
                     /translation="MTIKCLAFDLDGTLLLDDSKRMDPRTVDSIQRAMEQGVHIVIAS
                     GRDKNGCKFVYEPLRLEDGNHFLALVNGQVVYDFENKEYDLDDVLTPEDGIKIQKVCK
                     EFGVEGIFQCGYDFYSYISSLNRVKKKVKNAILGEPDDYGLKDGRENRNFIDLPFEEI
                     RLTQDINKVIMVHTPKFFEKNFDGIKAALSDYDVLLVGPDWLEIMPKHVSKASALWKI
                     CDKLGISMNEVMAFGDAQNDLKMLQQVGIGVAMGNAMDEAKYAATVVTDTNMNNGIGK
                     AIDALLAGKEMDLRKGLL"
     gene            6042..6350
                     /gene="trxA"
                     /locus_tag="ACZPDN_04135"
     CDS             6042..6350
                     /gene="trxA"
                     /locus_tag="ACZPDN_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1297843.1"
                     /translation="MKIVNTEEFNALMNEDAVLVDFFATWCGPCKMLTPVLESVANKL
                     DGKVKIVKVDVDESPDLAQRFGIMSVPTMILFKKGQQTAAFSGYMPEAMLMQNIESKL
                     "
     gene            6386..6796
                     /locus_tag="ACZPDN_04140"
     CDS             6386..6796
                     /locus_tag="ACZPDN_04140"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF017868.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR1297844.1"
                     /translation="MYYIAYGSNLNKEEMKQRCKDAKFVSLARLENYTLTYRGNPGRA
                     YLTVDKKKGDFVRVAVWDVSENDKKSLDEYEDYPYLYDCFDEKVLLENNETITGFIYQ
                     MYDRFPICKPSDSYMERCKQGYRDMGWDASILDF"
     gene            6850..7434
                     /locus_tag="ACZPDN_04145"
     CDS             6850..7434
                     /locus_tag="ACZPDN_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1297845.1"
                     /translation="MKNWRYLILSLVLSLFIHYYVTAVLMEIFGMGWYPIQFVVWCIE
                     LVVIYDALMFFTKNWNVKHRAMFFFAYYLFLIIALLIRYDQGITIIQLNPFACLREIY
                     YGSWTEWIVFAFNIGYFMFMPWVNGLFISSNKENFIVSVFIGFLCEFMQLIFHRGVFD
                     LGDISLYVVGILLGIYVKEKYHVHLKEKSEVHDN"
     gene            7424..8323
                     /locus_tag="ACZPDN_04150"
     CDS             7424..8323
                     /locus_tag="ACZPDN_04150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1297846.1"
                     /translation="MTIKTGAIFGAGAVGSYCIYGLSKCDIQLSVIAKDERNERLKKN
                     GCLINNIIYHPEVLTPEEAHGVDLLIVCLKYNALPDALEDIKQIVDEHTLVMSLMNGV
                     DSEQIIGNQIGMQHMVYSLIKVASHKEGLGYVFDPETTIGIVLEKNEEIEELLSQSDL
                     HYRTTSYIQEEIWSKFRLNVTKNLPQAILGTGVGCYSDSDHVKAIQSGLKEELEAIAN
                     AKGIDMSKADPSATRGSAVPKTARYSTLQDLDAKRHTEIDMFSGAIMKMGKELNIPTP
                     YNEFVYHVIKAMEEKNDGLFEYK"
     gene            complement(8338..8619)
                     /locus_tag="ACZPDN_04155"
     CDS             complement(8338..8619)
                     /locus_tag="ACZPDN_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297847.1"
                     /translation="MTTENTFKIILPNLNYQKKSIKIREDIVVYDCPENKILEFAKTH
                     TQFTRKDIQDTFNLSQTTCGRILNRMKEEGQISQHGKGKNTFYILNEKK"
     gene            complement(8616..9254)
                     /locus_tag="ACZPDN_04160"
     CDS             complement(8616..9254)
                     /locus_tag="ACZPDN_04160"
                     /inference="COORDINATES: protein motif:HMM:NF025124.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297848.1"
                     /translation="MIFQEDFEQMRSLEQDLTFLSAKAEFDESNIPFKEKNLGIQNQD
                     SIYTNLGLLLSDQCKHTIKVAVFNGETQNVFQDRNEFAGSLFKQMHEVYSYIDFRNQK
                     HSNFSGLKRIDKRDYPEVAIREALLNLIVHREYSYSASSFIRMYTDRIEFTSIGGLLK
                     GITLEDIQLGISVCRNPKLANIFYRLELIEAYGTGIQKILDSYSESESSLNL"
     gene            9455..9703
                     /locus_tag="ACZPDN_04165"
     CDS             9455..9703
                     /locus_tag="ACZPDN_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297849.1"
                     /translation="METIKDYVAHLDNKKRITLRGATYHYYNVKEYGNGCIILEPREL
                     SVPKSISARTLADMDRAVSNFKCGDVSTAIDLSDFSKE"
     gene            9707..10075
                     /locus_tag="ACZPDN_04170"
     CDS             9707..10075
                     /locus_tag="ACZPDN_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297850.1"
                     /translation="MNFNIRMGIPEMQELWQDLQQKYRSGKIKKKEEQLYKKWGKALK
                     LLSADPFYPSLQTHEIEPLSRRYGMKVWQSYLENKTSGAMRMYWVYGPDQKDITIIGL
                     EPHPEDKKNGAYDRISLSDL"
     gene            complement(10215..11711)
                     /locus_tag="ACZPDN_04175"
     CDS             complement(10215..11711)
                     /locus_tag="ACZPDN_04175"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR1297851.1"
                     /translation="MLKFEPFYQEYERTLDAFSLAFSTIYFEQMTIAPKKGIPYSNEM
                     LSRLSKQAFIIENDPETIKKIKEYAKTLPEGSLEKKEVDYRLEALAQSENIPSDVYAN
                     YVKVKADSELAWHEAKEADNYEHFKPHLQAIMKETLHLLEYDPKFNGNNAYDVLLDRY
                     EKGMTQEKYDAFFNNVKEKLVPLIHEIQNKPQINDDLLHETYDLDRQEKFMEVICDFM
                     KVDFAKVYLSTTEHPFTDFFSSNDARITTHYYPDQFLSAILSTVHEYGHALYGLQMDP
                     AFEGTMLTQAVGSAAHESQSRFLENHVGRSHAFWQANYNQLVNLFPEFKDVSVDELVN
                     MINKTECALIRTEADELTYPLHIMVRYEIEKEIAKGNVDYDKLPELWADKYEEYLGVR
                     PTNYKEGVLQDMHWSTGYLGYFPTYALGSAYAAQLYATMEKQFDVKEALLTNHFEKIT
                     DWLKENVHHYAASKSMAEIVEEVSGEPFNPDYYTDYLIKKYKKLYNLD"
     gene            11819..12319
                     /locus_tag="ACZPDN_04180"
     CDS             11819..12319
                     /locus_tag="ACZPDN_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1297852.1"
                     /translation="MDAIFYRTSVRKYKKESLKKEEIDLILKAAFSAPSARNSQPWQF
                     IVVENKDTLKDLSQMTPYASFLKDAAMGMIVCADKNKNDSLDYCQQDLAAATENMLVE
                     AKSLGIGSCWLGVYPNEDRYLALNQYFKLPKGIVPMWMISFGYIDQEEVVKDKFDSSK
                     VHYEKY"
     gene            12306..12815
                     /locus_tag="ACZPDN_04185"
     CDS             12306..12815
                     /locus_tag="ACZPDN_04185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1297853.1"
                     /translation="MKNINIVECKNEKDLKEVSILAEKIWHEFFPGIISEAQIDYMVE
                     QFQSFDAMQDQVKHQQYHYHKVYEGDELVGYIGLQNQPDRLFLSKLYLKDLARGKHYA
                     SKMFEYVKKQAEKYCYSSIYLTCNKYNKHSLAVYEKFGFQWIDSVQTDIGNGFIMDDY
                     ILEYQLNRI"
     gene            12883..13908
                     /locus_tag="ACZPDN_04190"
     CDS             12883..13908
                     /locus_tag="ACZPDN_04190"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1297854.1"
                     /translation="MKKIQELPTPEDLKKEFPLPYKVQKIKKQRDQEIEDIFTGKDDR
                     LILIIGPCSADREDAVLDYMNRLAVLQEKVKDKIFMIPRVYTNKPRTKGVGYKGMLHQ
                     PDPHSKEDMLKGIIAIRKLHTEVVTQTGFTCAEEMLYPENHAYLSDLLGYVAIGARSV
                     ENQQHRIVASGVGIPAGMKNPTGGDLTVMMNSIVAAQADQRFVYRGWEVQTDGNPLAH
                     AILRGYVDKFNHAHANYHYEDLMELIHLYEKSDLKNPACIVDCNHSNSNKQYLEQIRI
                     AKDVMHSRSKSADIKKLVKGLMIESYLEDGNQPTDGNVYGKSITDPCLGWDKTEQLIL
                     DLYELCD"
BASE COUNT         4591 a         1887 c         2936 g         4602 t
ORIGIN      
        1 tagtatacaa ggttgttcaa ctaaactaca agatgtgatc aaagttgttt ttgaatcaca
       61 agaacaatgt gttgagtttg ataaactgtt caaagaattt tcatatattc aaacttattc
      121 attcgaagaa aatggacatt tcaatggtct tattatggat aagaaataga aaagtataga
      181 cttaaaatgg tgcctacatc ctcgtaggta ctttttttct tttcatctgc attgaaaaag
      241 aataaaatat acaaaaaaag gagaatcctt atgacagaat tttatgaaca agtaaaagag
      301 agattgatgc gctacgcaag aatcaataca gaatccgatc cacatagtac aagtgttcct
      361 actacatctt gtcagcatga tttagctagt attatttatg aagagcttca aaaaattggt
      421 gctacaaatg tatattatga taaagataca tgtgtggtat atggatcttt agaagccaat
      481 gtgaaaacgg gtagagctat tggttttgtg acacatatgg atacagctcc agatgcgagt
      541 ggtaaagatg taaagccttg ggtgttagaa aattatgatg gaaaggacat tgttttaaat
      601 gctgaaaaag atattgtgat gtcaccgtct gtatttccaa atttgaaaca atatattggg
      661 caggatttga ttttaaccga tggtacaaca ctacttggag gggatgataa ggcaagtatt
      721 gcagctgtta tgacactatt ggaatattta gtgaaacatc cagaaattaa gcataatttt
      781 atttctgtcg catttacacc agatgaagaa gtaagtggac ttgctaaaga tttagattta
      841 gaacgcttta aatcacccat cgcatatacg ctagatggag atcatttggg ctattatatg
      901 gacgaaacat ttaatgcgag tctttcaacg attgaaatcc atggtatttc agtacataca
      961 gctaccgcta agggcattat gaaaaatgcg gtggatattg gaaatgcatt cttaaataaa
     1021 ttaccggcat tagaaaaacc acaatatacc caaggaagag aaggctttta tcatgtggtg
     1081 agttttaatg gagattgtga atatgccaag attgaaatca atgttcgtga tcatgacagt
     1141 ttacagtttg atattcgaaa caacacatta aaaatgattg tggatatgtt aaatgaagag
     1201 tatggacaag gtactgtaaa tattacacaa agaattcaat atcgcaattt aaaagaagta
     1261 atcgatcaag tgccattcat gattccatat ttgaaacaag ccattgaaag tgtaggatta
     1321 acccctcaaa cagaaccatt tagaggagga accgatggtt cagccttatc tcatagagga
     1381 cttccttgtc ctaacttatc agcgggatat gagaatgcgc atggacgttt tgaatatgtt
     1441 cccattcagt cgatggaaaa gaatgtggaa atcttattga atctaattga tatatatgcg
     1501 aaatgtgact gttaaaaagg tcacattttt tggttgcgta cagtttgaat ttttagtata
     1561 atacactcag tcgcagagat ggtgaaatgg cagacacgct gtcttcaggc ggcagtgcga
     1621 gagatcgcgt gagggttcaa atccctttct ctgcaccata taaattatgc cgacttagct
     1681 caattggtag agcaactgac ttgtaatcag taggttgtgg gttcaattcc tatagtcggc
     1741 accacttaaa caatatacag ccttaatggc tgttttttat ttttcttctt gttgtataat
     1801 gaatataaag aaatatgcat atcaaggaga aataataata tgaaagaagt atttgaagtg
     1861 ggtcttgata tcattaaaaa gattcaagaa acacaatctg aaaaattaga acaaaccggt
     1921 catttagtgg ccaaggcatt tatggatggc cataagtttt ttgtcagtgg aagtggacac
     1981 tctcatacaa tggctgaaga gatgtatgct cgtgcaggtg gcttagcttt tgtggttccc
     2041 attttgacaa gtgaacttac gatgacggag catccaacaa agagttcaca tattgaaaga
     2101 cttagtggtt atgccaaaat cttagcagag ttatatggta tatcatgtgg tgatgtcgtt
     2161 ttgatcgcat ccaattctgg gcgtaatgcc tatccaatcg aacttgcact tgaagctaaa
     2221 aatcgtggtg cacatgtagt tgccatcaca agtatgaaac atacaactgc tcaatcttct
     2281 cgtcattctt gtggaaagaa tttgtaccaa attgcagata ttgtcattga taattgtgga
     2341 gaagttggag attgtgcttt atctatggat ggtttggatg ccaagctttg tccaacatca
     2401 agtatggcca attctttcat tgcacaatgc atcaatgtgg catgtgcaaa gtatttgatc
     2461 gaacacaatg tagaagtacc tattttttca tctttaaact gtgatggtac ggaagaaaga
     2521 aatgacaaat tatttaaaaa gtatacgcgt atgtattaga ataaggagat gaggtgctta
     2581 tgaagaaaag aaatttatgg atatatatat ttggaatcac aatcattatt attggattga
     2641 ttaaattgga tacattgatt tctatggtat ctctattatg gaatgcatgt agagttttag
     2701 tgtttggagc tatgatggca tttgtgatca acttagttat ggccaaaatt gaaaagcgtt
     2761 tggattttat gaagaaagga aagcgcgcaa cgagtttact tctttctttg attttaattt
     2821 ttctttttgt gtatttctta attacactcg ttgttccatc attaagcgaa gccattggtg
     2881 taattgtaca agtattgcca aagtattttg aaaatgccca gaagttttta gtggatttgt
     2941 ttgagaacaa tccacagctg gcagattcta ttggtaattt ggatattgat tggaaaaatt
     3001 tgattcaatc cggtattaat gttctttcaa ccggtgttac caatgttgtg ggtacaactt
     3061 ttaatgtggt aaccgttatt gtgaatagtg tatttaactt tgtgttgatg tttatcttcg
     3121 caatttatgt tttattagaa aaagaaagat ttgttcaatt gtatcatcgt ttaacgaagc
     3181 tatatctaag tgaaactatg aagcgtagaa tggataaatg cttgcatatt attaatcaaa
     3241 catttagtgc ttttattggt ggacaatgta ttgaggctac cattttagga acactatgta
     3301 cattgggtat gattatattg cgtatgccat atccggcaat gattggaaca ttagttggtg
     3361 tcattaatat tattccgatg attggagctt atattggtgg ggcgattggt atgtttatgg
     3421 tatttactgt aagtcctatt atgtcacttt ggttcttatt atatttatgt attcttcaac
     3481 aaatcgaaag taatgtgatt tatccacatg ttgttggaaa ttcagtagga ttaccaggaa
     3541 tctatgtcat gatgactgtt gtgatttttg gatctttagc cggtattcca ggtatgtttc
     3601 taggaattcc aacaatggca agtgtgtata aaataggtgt tttatatcta agtcgaaaag
     3661 aacgtttaaa agaaatgaat ggatgatgaa aagtcgtcca ttttttctta tatgcatgat
     3721 ataatgttgt ggcaaaggag atcagttatg tttgatgaaa ttcatgaaga gtgtggtgtg
     3781 tttggtgcat atcgcgtaga taatgcagca agtattacgt attatggttt gcattccttg
     3841 caacatagag ggcaggaagc aagtggtatt gccgtttcgg atggtgagaa tattacttta
     3901 caaaaaggta aagggttgac ggtagatgtt ttccaaaaag aaaaattaga ttctatggtc
     3961 ggaagattgg ctgttggaca tgttcgttat agtacagctg gtggtcagga aaatgaaaat
     4021 attcagccaa ttgtatcaaa gggacataat ggaagtttag ctatcgttca taatgggcag
     4081 attgtaaatg aaaaagagct tcgtattgaa cttgaaaatg aaggcgcgat ttttcaagga
     4141 acaagtgata gtgaaattat tcttcatttg attcaaaaac aaaagggtac tttgaaagag
     4201 cgtgttatga aaaccgcaaa tcgtattgaa ggggcatttt ctttcttagt tatgaatgag
     4261 gatactattt atgcggttcg tgatcgtcat ggattaagac ctttgagtta tgcaaaaagt
     4321 aaagatggct atgtgatcag tagtgaaaca tgtgcttttg aagttatggg aatctatgaa
     4381 agtgtggatt tgaagccagg agaaatcgtt gaattccata agggcattgt aaaacatgaa
     4441 ttttattcaa cagacacaga tcatcacatg tgtgctatgg aatatattta cttcgcaaga
     4501 ccagatagtg tggtagaagg tattaacgta catgctttcc gtaagaccac aggatctatt
     4561 ttggctagag aagataaaga tttaaaggct gacatcgtta ttggtgtacc agactcatct
     4621 ttatcggctg ccattggtta tgcagaagag gcgggtattc catttgaaac aggtttagta
     4681 aaaaatagat atgtagggcg tacatttatt cagcctacac aggcaatgcg ggatcgtagt
     4741 gttcgattaa aactaagtcc ggtatctagt gtagttaaag gaaaatcaat tgttatgatt
     4801 gatgattcga ttgtgcgtgg tacgacttca agaagaattg ttcaattatt aaaagatgca
     4861 ggggctaccc aagttcatgt tcgtatcgca agtcctgtaa tcacaagtcc atgtttctat
     4921 ggtgtagata cttctacaaa agatcaattg attggtgctc gtatgagtgt agaagaactt
     4981 cgtgaatata tttgtgcgga tagtttgcgc tttatgacgg aagaagaaat gaaagaagcc
     5041 acgcatggtg ttggattatg tttggcatgc tttaatggag aatattgtac aaaattgttc
     5101 tcatatcaag aagaattgga taagtaatat gactataaaa tgtttggcgt ttgatttaga
     5161 tggaactcta cttttagatg attcaaaacg aatggatcca agaacggtgg attcgattca
     5221 aagagcaatg gaacaaggcg tgcacattgt gattgcgtcg ggtcgagata aaaatggatg
     5281 taaatttgta tatgaaccat tgagattaga agatggaaat cattttttgg cattagtgaa
     5341 tggacaagtc gtatatgatt ttgaaaataa agaatatgat ttggatgatg ttttaacacc
     5401 agaagatgga attaaaatac agaaagtatg taaggaattt ggcgttgagg gaatttttca
     5461 atgtggatat gacttttata gttacatatc ttcattgaat cgtgtaaaga aaaaagttaa
     5521 gaatgccatt ttgggtgagc ctgatgatta tggcttgaag gatggtagag aaaatcgtaa
     5581 ttttattgat ttaccttttg aagaaattcg cttaacacaa gatatcaata aagtgattat
     5641 ggtgcatacg ccaaaattct ttgaaaagaa ttttgatgga atcaaagcgg cacttagtga
     5701 ttatgatgtg ttgttggttg gtccggattg gcttgaaatt atgccaaaac acgtaagtaa
     5761 ggcaagtgca ttgtggaaga tttgtgataa attaggtatt agtatgaatg aggtcatggc
     5821 ttttggtgat gcacaaaacg atttgaagat gcttcaacaa gtaggtattg gtgtagctat
     5881 ggggaatgcg atggatgaag ctaagtatgc tgccactgtc gttacggaca caaatatgaa
     5941 taatggcatt ggaaaagcaa tcgatgccct gcttgcgggc aaagaaatgg acttacgtaa
     6001 aggcttgtta tagaatggaa ttagataagg aggaaccttg tatgaaaatt gtaaatacag
     6061 aagaatttaa cgcattaatg aatgaagatg cagttttagt cgacttcttt gcaacttggt
     6121 gtggaccatg taaaatgttg acacctgtac ttgaatcagt cgcaaataaa ttagatggaa
     6181 aagtgaaaat tgtaaaggtg gatgtggatg aaagtccaga tttagctcaa agatttggta
     6241 ttatgtctgt tccaacaatg attctattca aaaaaggaca acaaactgca gcattctcag
     6301 gatatatgcc tgaggcaatg ttgatgcaaa atattgaaag taaactatag gatgattcgt
     6361 catcctattt tctttagggg gaaatatgta ttatatcgca tatggaagta atttaaacaa
     6421 agaagaaatg aagcaacgat gtaaagatgc gaagtttgta tctttggcaa gacttgaaaa
     6481 ctatacactt acatatcgtg gaaatcctgg acgagcttat ctaacggtag ataagaaaaa
     6541 aggagatttt gtacgagttg ccgtatggga tgtgagcgaa aatgataaga agagtttaga
     6601 tgaatatgaa gactatccat acttatatga ttgttttgat gaaaaggtat tattagaaaa
     6661 caatgaaaca atcacgggct ttatctacca aatgtatgat cgttttccaa tatgtaaacc
     6721 aagcgattca tatatggaac ggtgcaaaca aggctatcgt gatatgggtt gggatgcttc
     6781 aattttagat ttttaatgaa ttttaaagtt tagtgtgtca taataagagg aaaataaggg
     6841 gactttaaaa tgaaaaattg gagatacttg attttaagtt tagttttatc cttgttcatt
     6901 cattactacg tcacagctgt tttaatggaa atttttggca tgggatggta tccaattcaa
     6961 tttgttgtat ggtgtattga actggtcgtt atatatgatg cgttgatgtt ttttacaaaa
     7021 aattggaatg taaaacatcg agcgatgttt ttctttgcgt attatttatt tttaattatt
     7081 gccctattga ttcgctatga tcaaggtatc actattattc agttgaatcc atttgcgtgt
     7141 cttagagaaa tctattatgg ttcttggaca gaatggatcg tattcgcatt taatattgga
     7201 tactttatgt ttatgccatg ggtgaatgga ctatttattt catcaaacaa agaaaatttc
     7261 attgtttctg tattcatcgg ttttctgtgt gaatttatgc agttgatctt tcatagaggc
     7321 gtatttgatt taggggatat ttctctatat gtagttggaa tcttgttggg gatttatgta
     7381 aaagaaaagt accatgttca tttaaaagaa aagagtgaag tacatgacaa ttaaaacagg
     7441 tgcgatattc ggtgcgggag ctgttggctc ctattgtatt tatggtttat caaagtgcga
     7501 tatacaatta agtgtcatcg caaaagatga gcgaaatgag agattaaaaa agaatggttg
     7561 tttaatcaac aatatcattt atcatcctga agtattaacg cctgaagaag cacatggagt
     7621 agatcttctc attgtatgtt taaaatacaa tgcgttgcca gatgctttag aagatattaa
     7681 acaaattgta gatgaacata ctttagtcat gtctttaatg aatggtgtgg atagtgaaca
     7741 aattattgga aatcaaattg gtatgcagca tatggtgtat tcattgatta aagttgcaag
     7801 tcataaagaa ggcttaggat atgtatttga tccagaaaca acgattggaa ttgtgcttga
     7861 aaaaaatgag gaaatcgagg aattgttgag tcaaagtgat ttacattatc gaacaacttc
     7921 atatattcag gaagaaatat ggagtaagtt tcgtttaaat gtaacgaaaa acctacccca
     7981 agccattctt ggaactggtg ttggatgtta ctcggatagt gatcatgtga aagctataca
     8041 gtctggatta aaagaagaat tagaagccat agccaatgcg aaaggcatag atatgtcaaa
     8101 ggctgatcca agtgcaacac gtggtagtgc agtgcctaaa acagccaggt attctacact
     8161 acaggatttg gatgccaaaa gacatacgga aattgatatg ttttcgggtg caataatgaa
     8221 gatgggaaaa gaattaaata ttccaacgcc ttataatgaa tttgtgtatc atgtcatcaa
     8281 ggctatggaa gaaaagaatg atggtttgtt tgagtataaa taaagagaag tgaggaatca
     8341 cttcttttca tttagtatat agaatgtatt tttaccttta ccatgttgag agatttgacc
     8401 ttcttctttc attctgttta aaatgcgccc acaagttgtt tgagaaagat tgaaggtatc
     8461 ttgaatatct ttacgagtga attgggtatg tgttttcgca aactctaata ttttgttttc
     8521 aggacaatca tatacaacga tatcttccct aattttaata ctcttttttt gataatttag
     8581 atttggaaga ataattttaa aggtattttc tgttgtcaca aattgaggct tgattcagat
     8641 tctgaatacg aatcaagaat cttttgaatg ccagtaccat aagcttcaat gagttctaac
     8701 cgataaaaga tatttgcgag ttttgggttt cgacaaacag aaataccaag ctgaatgtct
     8761 tctaaagtga ttccttttaa taagcctcca atggaagtga attcaatccg gtctgtatac
     8821 atacgaatga aggaacttgc actatatgaa tattcacgat gaacaattag gttcaacaaa
     8881 gcttctctta tagctacttc tggataatct cttttatcaa ttcttttcaa tcctgaaaag
     8941 ttggaatgtt tttgatttct aaaatcaata taagaatata cttcgtgcat ttgtttgaat
     9001 aatgaaccgg caaattcatt tcgatcttga aaaacattct gcgtttctcc gttaaaaaca
     9061 gctactttaa ttgtgtgctt acattgatcc gataaaagta gtcccaaatt cgtgtatata
     9121 ctatcttgat tttgaatgcc taagttcttt tctttaaatg gaatgtttga ttcatcaaat
     9181 tcagccttgg cagaaagaaa ggtcaagtct tgttctagag aacgcatttg ttcaaaatct
     9241 tcttgaaata tcataaataa agctcctttt tacccatata ctaaccaatc taaccattct
     9301 aatcaataaa gaatttgttt ttcttgttgt gcatatcccc cttcctgatg atagtgaaca
     9361 agaaaaatta gattgacaaa gaataaagaa aaaattatta taatgatagg gagtgggaat
     9421 tcccacgttt agatttgtat tggaggtgtt gataatggaa acaataaagg attatgttgc
     9481 acatttggat aacaaaaaaa gaattactct tagaggagct acttatcatt actacaatgt
     9541 gaaagaatat ggaaatggtt gtataattct tgaacctcgg gaattatcag tgccaaagag
     9601 catttcagct agaacattgg cagatatgga tcgtgcggta agtaatttta agtgtggaga
     9661 tgtttccacg gctattgatt tgtcggattt ttcaaaggaa taataaatga attttaatat
     9721 acgaatggga attcctgaaa tgcaggaact ttggcaagat ttgcaacaaa aatatcgttc
     9781 tggtaagatc aaaaagaagg aagagcaact ctataaaaaa tgggggaaag ctttaaagtt
     9841 gttgtcggcg gatccatttt atccgagtct tcaaacccat gaaatagagc ctttatctag
     9901 acgttacgga atgaaagtgt ggcaatctta tttggaaaat aaaacaagtg gtgcaatgag
     9961 aatgtattgg gtttacggac cggatcagaa agatatcacg attattggat tggagccaca
    10021 tccggaagat aagaaaaatg gcgcatacga tagaatttca ctgtcggatt tgtagaataa
    10081 atttgaataa ggtactaaat atgagtagtt agtctgtgaa aataggttag ctgctttttt
    10141 tcttaaaaat tcagaatgat tcatttctaa ccattcttgt caataagaaa agaagtgatg
    10201 ttactcactt cttcttagtc tagattgtat aatttcttgt atttcttaat taaataatct
    10261 gtgtaatagt ctggattgaa tggttcacca cttacttctt ctacgatttc agccatcgat
    10321 ttacttgcag cataatggtg tacattttct tttaaccagt ctgtaatctt ttcaaaatga
    10381 tttgttagta gggcttcttt tacatcaaat tgtttttcca ttgttgcata caattgtgca
    10441 gcatacgcac tacccaatgc gtatgttggg aagtatccta agtatcctgt agaccagtgc
    10501 atatcttgta agacaccttc tttatagttt gtaggacgaa cacctaaata ttcttcgtat
    10561 ttatcggccc atagttctgg taacttatca taatctacat tgcctttcgc aatttctttt
    10621 tcgatttcat aacgaaccat gatgtgtaat ggatatgtca attcatcggc ttctgtacga
    10681 atcaaagcac attctgtttt attgatcatg ttgactaact cgtcaacaga cacatcttta
    10741 aattctggga ataaatttac taattgatta tagtttgctt gccagaaagc gtgacttcta
    10801 ccaacgtggt tttctaagaa acgagattgc gattcgtgtg cagccgaacc aacagcttgt
    10861 gtaagcatgg ttccttcaaa tgcaggatcc atttgtagtc cataaagagc atgtccatat
    10921 tcgtgtacag ttgaaaggat agcgctcaaa aattggtctg gataatagtg tgttgtgata
    10981 cgagcgtcgt tgcttgagaa gaaatctgtg aatgggtgtt ctgtggttga aaggtatact
    11041 ttagcaaaat ctactttcat gaaatcacag attacttcca taaatttttc ttggcgatcc
    11101 agatcataag tttcatgaag taagtcatca ttgatttgtg gtttgttttg aatttcatga
    11161 atcaatggaa ctaatttttc tttcacatta ttaaagaatg catcatattt ttcttgtgtc
    11221 attccttttt cgtaacggtc taacaataca tcatacgcat tatttccatt gaatttagga
    11281 tcatattcta aaaggtgtaa agtctctttc atgatggctt gtaaatgcgg tttgaaatgt
    11341 tcatagttat ctgcttcttt agcttcatgc caagcaagtt cactatccgc tttgactttg
    11401 acatagttcg catatacatc actaggaatg ttttcagatt gagctaatgc ttcaagacga
    11461 taatcgactt ctttcttttc taaacttcct tctggcaatg tctttgcgta ttccttgatc
    11521 tttttgattg tttctggatc attttcaatg atgaatgctt gtttagatag tctagacaac
    11581 atttcatttg aataaggaat tcctttcttt ggtgcaattg tcatttgttc aaaataaatg
    11641 gttgagaatg ccaatgagaa ggcatctaat gttctttcgt attcttgata gaatggttca
    11701 aattttaaca tggttaaatt cctcctgctt gtattgtaac acaaagaaaa gaatagtcat
    11761 tatataatga aagcgaattt tcaaaatggt gatataatac agaaaaggag tatagagtat
    11821 ggatgcaatt ttttatcgta caagtgtaag aaaatataaa aaagaatctt taaaaaaaga
    11881 agagattgat ttaattttaa aggcagcatt tagtgcacct agtgctagaa atagtcagcc
    11941 ttggcagttt attgtcgttg agaataaaga tacattaaag gatttaagtc aaatgacacc
    12001 ctacgcatct tttctaaaag atgcagctat gggaatgatt gtttgtgcgg ataaaaataa
    12061 gaatgatagt ttagattatt gccaacaaga cttggcagcg gctacagaga atatgttggt
    12121 ggaagcgaaa agtttgggta ttggttcatg ctggctgggt gtgtatccga atgaagatcg
    12181 ttatttggct ttaaaccagt attttaaatt accaaaagga attgttccca tgtggatgat
    12241 ttcatttgga tatatagatc aagaagaggt tgtaaaagat aaatttgata gttcgaaggt
    12301 gcattatgaa aaatattaat attgtagagt gtaagaatga aaaggattta aaagaagtat
    12361 ctattcttgc cgaaaagatt tggcatgaat tttttcctgg tatcatttca gaagcacaga
    12421 ttgattatat ggttgaacag tttcaaagtt ttgacgccat gcaagaccaa gtcaaacatc
    12481 agcagtatca ttatcataag gtatatgaag gggatgagct agttggatat attggtttgc
    12541 agaatcagcc ggatcgttta ttcttgtcta aactgtattt aaaggatttg gctagaggaa
    12601 agcactatgc atctaaaatg tttgaatatg taaagaaaca ggcagaaaag tattgttact
    12661 catctattta tttgacttgc aacaaatata ataagcatag tctagctgta tatgagaagt
    12721 ttggttttca atggattgat tctgtacaga cagatatagg aaatggcttc atcatggatg
    12781 attacatctt ggaatatcaa ttaaatcgta tctgattttg aaaaacatgc gagaatatga
    12841 tattgtcaaa gggtaattta aagagggatg ttgaatatgg atatgaagaa gatacaggag
    12901 ttaccaactc cagaagattt gaaaaaggaa tttcctttgc cttataaggt gcaaaaaatt
    12961 aagaaacaaa gagatcagga aattgaagat atttttactg gaaaagatga tcgtttaatt
    13021 ttaattattg gtccttgtag tgcggatcgt gaagatgcgg ttttagacta tatgaatcgt
    13081 ttggctgtat tacaagaaaa agttaaagat aagattttta tgattccacg tgtctacaca
    13141 aataaacctc gtacaaaggg tgttggatat aaggggatgt tgcatcagcc agatcctcat
    13201 tcgaaagagg atatgttgaa gggaatcatt gcgattcgta agctgcatac agaggttgta
    13261 acacaaactg gttttacttg tgcagaagag atgttgtatc cagaaaatca tgcgtattta
    13321 tcggatttat taggatatgt tgccatcggt gcaagaagtg ttgaaaatca acaacatcgt
    13381 attgtagcga gtggtgtagg aattcctgcg ggaatgaaaa atccgacagg tggagatctt
    13441 acagttatga tgaattctat tgtggcggct caagccgatc aaagatttgt atatcgtggt
    13501 tgggaagtac agacagatgg gaatccacta gctcatgcga ttttaagagg atatgtggat
    13561 aagtttaatc atgcacatgc gaattatcac tatgaagatt taatggaatt gattcactta
    13621 tatgaaaagt cagatttaaa gaatccggca tgtattgtgg attgtaatca ctcgaattca
    13681 aataaacaat atttagagca gattcgtatt gccaaggatg taatgcattc tagaagtaaa
    13741 tcggcagata tcaaaaagct agtgaagggc ttgatgattg agagttattt agaggatgga
    13801 aatcagccaa cagatggtaa tgtgtatgga aagtcaatca cagatccttg tcttggttgg
    13861 gataagacag aacaattgat tttagattta tatgagttgt gcgattagga agcgtgataa
    13921 aatggtaccc ataaggagga caacaacgtg acagttgtga tactcacttg tgggtatctt
    13981 ttttcgttgt tttaaaataa atatgtcaag agccaa
//
LOCUS       DBMDWL010000039        13780 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1506_length_13780_cov_3.153081, whole genome shotgun sequence.
ACCESSION   DBMDWL010000039 DBMDWL010000000
VERSION     DBMDWL010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13780)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_13780_cov_3.153081"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            226..1428
                     /locus_tag="ACZPDN_04195"
     CDS             226..1428
                     /locus_tag="ACZPDN_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1297855.1"
                     /translation="MNIFKKIYCRIFQNCFKLVIPVLPYRTPEILKSTSLISHICMDN
                     QISKVMVITDATVRQLGILDRMCEHLDHHSIEYVIYDKTVANPTSDNVEEAKNLYLSS
                     NCQALIGFGGGSSMDCAKAVGARLARPSRPLSKMEGILKIWKKLPLLICVPTTAGTGS
                     ETTLAAVIVDSKTRHKYAINDFYLIPDYAVLDPKPTLSLPPFITACTGMDALTHAIEA
                     YIGNSTTKKTREYALKATKLIFENIYKVYEDGNDLKARSNMLDAAYYAGHAFTVSYVG
                     YVHAIAHSLGGKYNIAHGLANATLLDVVLKQYGDSVHKKLYEMALYCGICNAKDSYKV
                     GAERFIEQITLMKKYFGISSTFSELKKSDIHELAVYADHEANPLYPVPVLWNVKELEK
                     VYDLVVED"
     gene            1431..2777
                     /locus_tag="ACZPDN_04200"
     CDS             1431..2777
                     /locus_tag="ACZPDN_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR1297856.1"
                     /translation="MDSIVLKQRAFYNQHKTLDIHFRINALKTLKKAILAYEDRINEA
                     IKMDVGKSNFESYMCEVGLALSELTYMEKHVHKFSKEKTVYTPLSQFLSRSFEKPSPY
                     GVVLVISPWNYPFLLSIDPLIDALAAGNTVVLKPSEFSPYTSQVIKDMIDEYFDPEYV
                     ACVLGDSEVSSKLLDSKFDYIFYTGSKRVGKIVMNKASQYLTPVTLELGGKSPCIVDK
                     DVDVKLAARRIVFGKYLNCGQTCVAPDYMLVHESIKDRFIECVISEIKRQYANLDDYG
                     HIINEKHYKRILGLIDMDKVVYGGKHHNLQIEPTVVDRVDFSDPIMQEEIFGPVMPIL
                     TYSNLDVVIDKINSMPSPLALYIFTNNKQVSKKVTSEVSFGGGCINDVVIHLATSNMG
                     FGGVGESGMGSYHGKRGFDTFSHTKSIVDKKRIIDLPMRYQPYTKLNEKLIRFFLR"
     gene            2794..3279
                     /locus_tag="ACZPDN_04205"
     CDS             2794..3279
                     /locus_tag="ACZPDN_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297857.1"
                     /translation="MEKKEKFEYDIDLGAIMDYVENRFMLVIKDEDWTQEEIEMLNSG
                     IDLHFCYTNDIAIFVLEGGDIDNSDFYFNVQECDWKDHLFASDCLDVDIILLNKANEI
                     CFKKSKTLTKEQSQIIKDCLKQQNEVSFMPSEYDVNVQGIQSAYEPYELVRFEKCAIK
                     L"
     gene            3291..3560
                     /locus_tag="ACZPDN_04210"
     CDS             3291..3560
                     /locus_tag="ACZPDN_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1297858.1"
                     /translation="MKAVISVIGKDKVGILAMIANECANYNLNVLDVTQTIVDSMFTM
                     TMMVAIDEMSIPLNEFAERMENLGKEKNLVIRCMHQDIFNSMHKI"
     gene            3573..4928
                     /locus_tag="ACZPDN_04215"
     CDS             3573..4928
                     /locus_tag="ACZPDN_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1297859.1"
                     /translation="MQTNEILETIKMIDEEYLDIRTITMGISLLDCMDPDIHVSCQKV
                     EEKICRLAKDLVKVGNDFSKTYGIPVVNKRISVTPIAMLVACSGGNPVEYALTLERCA
                     QKLGVDFIGGYSALVQKGYAAGDNALIESIPEALAKTQHVCASVNVGSTKAGINMDAV
                     AQMGRIVKCCAEYTKDDNCLGAAKLVVFCNAPEDNPFMAGAFHGVGEADCVINVGVSG
                     PGVVRAVLSKADKSLRMDQIADLIKRTAFKITRMGQLVGTVASEKLGVPFGIVDLSLA
                     PTPAIGDSVAYILEEMGLETCGAYGTTACLAMLNDAVKKGGVMATSNVGGLSGAFIPV
                     SEDAGMIHAARTGVLKLEKLEAMTAVCSVGLDMIVIPGETSASVISGIIADEAAIGMV
                     NTKTTAVRVIPAIGKDVGEELDFGGLLGAGPVMPISQISNDVMINRGGRIPAPLQALK
                     N"
     gene            5059..5409
                     /locus_tag="ACZPDN_04220"
     CDS             5059..5409
                     /locus_tag="ACZPDN_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR1297860.1"
                     /translation="MTIEECYIKMGANYQDVIKRLPSVSMIEKFALKFREDTSFQELE
                     KALNDKQVEIAFRAAHTLKGVCMNLGFDHLYKPSFEITESLRAGNLELALQQFDAVKE
                     QYTKTIDALNEFAA"
     misc_binding    5505..5712
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5816..6577
                     /locus_tag="ACZPDN_04225"
     CDS             5816..6577
                     /locus_tag="ACZPDN_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1297861.1"
                     /translation="MKKIIKLGMAALTALTMAGCSSESEDVKTITVGISPDYAPYESE
                     NKKGDIVGFDPDMMKCFEEYLSEEEGVTYKLEMKKMDFDNIITQLQGDQIDVGISGFT
                     YDSKRKVEWSDPYLGSSQVAVVVKDSDIASTSDLEGKSLVAQTGATGETAAKEVKDAK
                     VTGLKNVQDIMNALSANQYDAAIVDLGVAKNYVKSGEFKMLDESLMDEQNYIIAKKGN
                     TDVIDKMNTCIKKFLASEDYAKLCKKYGLSPLETK"
     gene            6598..7281
                     /locus_tag="ACZPDN_04230"
     CDS             6598..7281
                     /locus_tag="ACZPDN_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1297862.1"
                     /translation="MFVAIDQVFTLENILYLAKGALLSVAIAALSLLIGLVLGILGAS
                     GRRSKHKVPRAISFVYVTIIRGTPLLLQILIIFSVIPSIYTAFTGHVLRINPIVIGMI
                     ALSINSGAYQTELLRSGINGVDKGQWEACETLGLSNWKTMKLVILPQAFKRVVPPLIS
                     EFITLIKDSSLISCIGAVELLKGAQVIGAEYYDVMSPYMLAAIFYLIMTCIISCIGNK
                     VEKRLAVSD"
     gene            7274..8005
                     /locus_tag="ACZPDN_04235"
     CDS             7274..8005
                     /locus_tag="ACZPDN_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1297863.1"
                     /translation="MIRVENVSKQFNKTRALKNVSLEVNKGDIISLIGPSGSGKSTLL
                     RCIHGLEHVDSGKIYMDDEWMNPDDEKKFRVQRNRMGFVFQHFNLFPNMSVLQNCKLA
                     QVEVLNKTDEEAEKTALEYLDKVGLLEKKDAYPNNLSGGQKQRVAIARALCMNPDIML
                     FDEPTSALDPEMIKEVLEVMKDLGKQGMTMVVVTHEMGFARKVGTRVVFLDQGEIIEE
                     NTSEEFFANPQSDRAKDFLSKVFYE"
     gene            8002..9093
                     /locus_tag="ACZPDN_04240"
     CDS             8002..9093
                     /locus_tag="ACZPDN_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1297864.1"
                     /translation="MRLPDFKVEQWMNDYEKDAIYNMTDTCVKALTLKELLDCEDFDF
                     NELTLDYGQITGNVELKKEILSLYASGTIDNITTAQGCLQANELVMNTLLEKGDEVIC
                     VVPGYQQFVDIPKSIGCKVRLIELDEYDWQVGVKKFRKMLNASTKMIILNNPSNPTGT
                     EYSIDFLNELIEVCKPYGTYILCDEVYRGLNQEVSISDIYENGISTSSLSKVFSLAGL
                     RLGWIKANEYVIHQINVRRDYSMISTGPLVDKLGWIALKHKDELILRAKKIISSNKSI
                     VCEWLKENPRFECVLPSGGTVCFLKYYFDLKSETFSKLLLAEKGVFFVPGSCFDKEYY
                     FRLGLAQDSKLFKSGLDELSNFVDEHLIS"
     gene            9123..9635
                     /locus_tag="ACZPDN_04245"
     CDS             9123..9635
                     /locus_tag="ACZPDN_04245"
                     /inference="COORDINATES: protein motif:HMM:NF018676.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HIR1297865.1"
                     /translation="MRIQYLLCISLLLCGCTKVSDLTHQKSKSTEKAPVTVKKTNKVK
                     VIDCKNDSSQQVTFEAKGDKIQKMTQKFTMSFSDLGITEDMNSEQIQSKINSSLDDKY
                     NSIEGVHVSTQLKDKQVEVTLEMDFKTANIDMLIENNLLDKGEAQSDYVSLKKTQNAF
                     KKEGFACITK"
     gene            9679..10299
                     /locus_tag="ACZPDN_04250"
     CDS             9679..10299
                     /locus_tag="ACZPDN_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1297866.1"
                     /translation="MRYYIADTHFFHRSLNEKMDQRGFDDIDQMNEYMIDQWNSKVRK
                     NDEVVVLGDFSWGNAKETTEILDELKGKIYLIRGNHDRFLDDKNFDTSRFVWIKDYAE
                     LNENKRKVVLSHYPIACYDGQYRRDEFGNPKTFMLHGHIHATQDQQFLDAYQDYVRQQ
                     KHPRISDGQLENVPCQLINCFCMYSDYVPMTLDEWIEIDKQRRSVL"
     gene            10332..11054
                     /locus_tag="ACZPDN_04255"
     CDS             10332..11054
                     /locus_tag="ACZPDN_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1297867.1"
                     /translation="MHKLDTTPGHLPYYKQIKEFYKKDIQEGVLAVGDRVDSEMEIQE
                     MYDVSRITARQAILELEQEGMVKRGRGKGTFVTYRPKIKEELSHIRSFTDEMTALGRT
                     PGTKSFHITKEIPDQATRELFGLDEEVMYCVRRVRTADDVLLVYFVSYFPLDLNIPLN
                     MEEQTQSIYEVIGAPTRIDEEFSAINPSEEICLALKIRKDQPVLARKRVSYDKKKKKM
                     EYTMCYYRGDLYSYTISTSQKL"
     gene            11051..11887
                     /locus_tag="ACZPDN_04260"
     CDS             11051..11887
                     /locus_tag="ACZPDN_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1297868.1"
                     /translation="MILTIDIGGTNIKYGLCDKSGNIQQKGEYPTLDTADKIVQSICD
                     LPFEYTGIAISMPGIVNEDHSKAFITGKLSFLSNHPLKKRLEEIKGCKVTIENDGRCA
                     TWAELGYGNLINSKNALVVVLGTYIGMGIVIDHKVYEGSHLLAGEYSDAHMDTEKTFK
                     NTMAAYFGKDGLERATGLTGKELFDHLDDQKVHEGLNQYCQNLAWMLSNIQLLLDVDT
                     ILIGGGISAQPRLIECIKESMNNVKLWFCTLKMPEIQACKFNNDANLLGALYYYNNTE
                     KH"
     gene            11924..13288
                     /locus_tag="ACZPDN_04265"
     CDS             11924..13288
                     /locus_tag="ACZPDN_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297869.1"
                     /translation="MKSLIGDKKFYRMVLIVCIPIMIQNGFTNLASLLDNIMIGQLGT
                     LSMSGVSISNQLFQVFNVSVFGAMSGAGIFLAQFYGRGNRDGVHNCFRIKMLLCILIS
                     ILAICIFKLLGPSLISLYLNDDPTDSMTTLSFGKQYMDVMLIGLIPFAITQVYSSSLR
                     ETGNTVLPMKASVIAVIVNFCINYILIFGHFGFPKLGVIGAAIGTVVSRIVEMGINIA
                     AGLNNNYLRDAMRLYKISGDIFKNVVKRGMPLLCNEILWSISIALISQCYSTRGLFAV
                     AAINVTTTVTNFFMIICYAMGNSISIIVGQRLGAGEIEYAKDCDLKMVFMNFMMCLCI
                     GVALFGCSSFIPQIYNVSIEVKSLATSLLRVSACMLPIISLYYSSYFTMRAGGKTLLT
                     FFFDSGYTFIFTFMVALCLTRFTNLPIFNVYLLVQCVDIPKAVLGITLVKKGIWVHNI
                     VNEL"
     gene            complement(13359..13434)
                     /locus_tag="ACZPDN_04270"
     tRNA            complement(13359..13434)
                     /locus_tag="ACZPDN_04270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13399..13401),aa:Glu,seq:ttc)
BASE COUNT         4648 a         1699 c         2982 g         4451 t
ORIGIN      
        1 gaacaaggta gtgagaaaaa agagcttgaa atgtatcaaa aaatgatgga caagggattt
       61 gaaattaaag ctatcgcatc tatcttctca gtaagtgaag aatcaattcg aaaactattg
      121 atgaaagcat aaaggtatgg acatgtaggt gtccatattt tttatagttg acaaagtgaa
      181 caaatgaata taaagtatac aaagttaact aaacggaggt gctttatgaa tatattcaaa
      241 aaaatatatt gtcgaatttt tcagaattgt tttaaacttg tgattcctgt tttaccctat
      301 cgaactccag aaatattaaa aagtacatct ttaatttctc atatttgcat ggacaatcaa
      361 atttctaaag tcatggttat tacagatgct acagtacgtc aattaggaat attagatcgt
      421 atgtgtgaac acttagatca tcattctatt gaatatgtga tttatgataa gacggtcgca
      481 aatcctacaa gtgataatgt agaagaggca aaaaacttat atttatcttc gaattgtcag
      541 gcgttaattg gttttggtgg aggatctagc atggattgtg ctaaggccgt tggtgctaga
      601 cttgctcgcc caagcagacc attatctaag atggagggaa ttttaaagat atggaagaaa
      661 ctgccactac ttatttgtgt acctaccaca gctggaacag gaagtgaaac aactcttgcg
      721 gctgtcattg tagattcaaa aacacgtcat aaatatgcga tcaatgattt ttatttgatt
      781 cctgattatg ccgttttaga tcctaagcct acattatctt tgcctccatt tattacggca
      841 tgcacaggga tggatgcatt aacccatgcg attgaggctt atattggtaa ttcaaccaca
      901 aagaagacgc gtgaatatgc gttaaaggct acaaaactaa tttttgagaa tatatataag
      961 gtgtatgaag atggaaatga tttaaaagct agaagcaata tgctcgatgc tgcttactat
     1021 gcaggacatg cctttactgt atcctatgta ggatatgtgc atgcgattgc tcattcatta
     1081 ggtggtaaat ataatattgc gcatggacta gctaatgcga cattacttga tgttgtgttg
     1141 aaacaatatg gagacagtgt gcataagaaa ctctatgaaa tggctttgta ttgtggtatc
     1201 tgtaatgcga aggattctta taaggttggt gctgagcgtt ttatagaaca aatcacttta
     1261 atgaaaaagt attttggcat ctcatctaca tttagtgaat taaagaaatc ggatatacac
     1321 gaattggcag tgtatgcaga tcatgaagcc aatccattat atccagttcc agttttgtgg
     1381 aatgtaaaag agttagaaaa agtgtatgat ttagtagtgg aggattgagt atggattcaa
     1441 tcgttttaaa acaaagagcg ttttataatc aacataaaac gctcgatatt cattttagaa
     1501 ttaatgcctt aaaaacttta aaaaaggcaa ttttagctta tgaagacaga attaatgaag
     1561 ctattaaaat ggatgtagga aaatcaaatt ttgagtctta tatgtgtgaa gttgggttag
     1621 ctttaagcga attaacttac atggaaaaac atgttcataa attttcaaaa gaaaaaacag
     1681 tttatacgcc attatctcaa tttttatcac gtagctttga aaagcctagt ccttatggtg
     1741 tggtgttagt cattagtcct tggaattatc cttttttact ttctattgac ccattgattg
     1801 atgcacttgc agctggaaat acggtggtat taaaacctag tgaatttagt ccatacacat
     1861 cacaagtgat caaagatatg attgatgaat atttcgatcc agaatatgta gcttgtgtac
     1921 taggagatag tgaagtgagt tctaaattac tggattcaaa gtttgactac attttctata
     1981 cagggtcaaa acgtgtagga aaaattgtga tgaataaagc tagccaatat ttaacacctg
     2041 tcactctaga attgggagga aaaagtcctt gtatcgttga taaggatgtt gatgttaaat
     2101 tggcagcacg aagaattgtg tttggaaaat atttgaattg cggacaaact tgtgtggcac
     2161 cagactacat gttagtacat gaatcgataa aagacagatt tatcgaatgt gtgatatccg
     2221 aaattaagcg tcaatatgcg aacttagatg attatggtca tattattaat gaaaagcact
     2281 ataagaggat tttaggattg attgatatgg ataaggttgt ttatggtgga aaacatcata
     2341 atctacaaat tgaaccaacg gtagtggatc gtgtggattt ttcagatcca attatgcagg
     2401 aagagatatt tggccccgtt atgccaatat taacatattc aaatttagat gtagttatag
     2461 ataaaatcaa ttccatgcct tctcctttag ctttatatat tttcacaaat aataaacaag
     2521 tatcaaagaa agtaacgagt gaagtttcat ttggtggagg ttgcatcaat gatgtggtga
     2581 ttcatttagc tacaagcaat atgggctttg gtggtgttgg tgaaagtggt atgggttcat
     2641 accatggaaa gcgtggtttt gatacgttta gtcatacaaa gagtattgtg gataaaaaaa
     2701 gaatcattga tttacctatg cgttatcagc cttatacaaa attgaatgag aaattgattc
     2761 gctttttctt aagataatct ttataatgaa tgtatggaaa aaaaagaaaa atttgagtat
     2821 gacatcgatt taggtgccat catggactat gtagaaaacc gttttatgtt agtaataaaa
     2881 gatgaggatt ggacacagga ggaaattgaa atgttgaata gtggtattga tcttcatttt
     2941 tgttacacaa atgatattgc gatatttgtg cttgagggtg gagatattga caatagtgac
     3001 ttttatttca atgttcaaga atgtgattgg aaagatcatt tgtttgcgag tgactgctta
     3061 gatgtcgata ttattttact aaataaggca aatgagattt gttttaaaaa gtcaaaaact
     3121 ttgacaaaag aacaaagtca aattattaag gattgtctaa aacaacaaaa tgaggtatcc
     3181 tttatgccaa gtgaatatga tgtgaatgtg caaggaatcc agagtgcata tgaaccatat
     3241 gagctagtgc gttttgaaaa atgtgcaata aaactataag gagaatgatg atgaaggctg
     3301 ttattagtgt aattggaaaa gacaaagtag gaattttggc tatgattgct aatgaatgtg
     3361 caaactacaa tttaaatgta ttggatgtaa cacaaacaat tgtagattca atgtttacca
     3421 tgactatgat ggtggcaatt gatgaaatga gtattccatt aaatgaattt gcagaaagaa
     3481 tggagaactt gggaaaagaa aagaacttag taattcgttg tatgcatcag gatattttca
     3541 attctatgca caaaatttaa tgaggtgttc ctatgcagac aaatgaaatt ttagaaacaa
     3601 taaagatgat tgacgaagaa tatttggata ttcgtacaat cacaatgggt attagtttat
     3661 tggattgtat ggatccagac attcatgttt catgtcaaaa ggttgaagaa aagatttgcc
     3721 gcttagctaa agacttagtt aaagtaggga atgatttcag taagacttat ggtattccag
     3781 ttgttaataa gagaattagt gtaacaccaa ttgcgatgtt agtggcttgt tctggtggaa
     3841 atccagttga atatgcatta actttagagc gttgtgctca aaaattaggt gtagatttta
     3901 ttggtggata ttctgcactc gtacaaaaag gatatgcggc aggagacaat gcgttgattg
     3961 aatccattcc tgaagccttg gcgaaaacac aacatgtttg tgcaagtgtt aatgtaggta
     4021 gtacaaaagc tggaatcaat atggatgccg ttgcacaaat ggggcgtatt gtgaagtgtt
     4081 gcgcggaata tacaaaggat gataattgct tgggtgctgc aaaattagtt gtattctgta
     4141 atgcacctga agataatcca tttatggcag gagctttcca tggtgtagga gaagctgatt
     4201 gtgtaatcaa cgttggtgta tccggacctg gtgttgtgcg tgctgtatta agtaaagccg
     4261 ataaatcatt gcgtatggat cagattgcag atctaatcaa gcgtacagct tttaaaatta
     4321 cgcgtatggg acaattagtt ggaactgttg cgagtgaaaa attgggtgtt ccatttggaa
     4381 ttgtggactt gtctttggca ccaacaccag caattggtga tagtgttgcc tacattttag
     4441 aagaaatggg attggaaaca tgtggtgctt atggaacaac tgcctgtctt gcgatgttga
     4501 atgatgctgt taaaaaaggt ggcgttatgg caacaagtaa tgttggtggt ctatctggtg
     4561 ctttcattcc tgtaagtgaa gatgcaggta tgattcatgc ggcaagaaca ggtgtattaa
     4621 aattagaaaa attagaagca atgaccgctg tatgtagtgt tggattggat atgatcgtaa
     4681 ttccaggaga aacaagtgca agtgttattt caggaattat tgcggatgaa gcggcaatcg
     4741 gtatggttaa cacaaaaacg acagctgttc gtgtgattcc agctattgga aaagatgttg
     4801 gagaagaatt agattttggt ggtttattag gtgcaggtcc tgttatgcca atttctcaaa
     4861 tttcaaatga cgttatgatc aatcgtggcg gaagaattcc agctccttta caagcgttaa
     4921 aaaactaatg aggaagtgtt ttacttcctt tttatattgc tttttctttg gttagattat
     4981 agaatatatg tgttttaaaa aagctttaat taaaaatgtt aatttaatat tcagttatat
     5041 tatagagagg aaaatagaat gacaattgaa gaatgttata taaaaatggg tgctaattat
     5101 caagatgtta ttaaacgttt accaagtgtt agtatgattg agaaatttgc actaaaattt
     5161 agagaagaca cgagttttca agaacttgaa aaagctttaa atgataaaca agtggaaata
     5221 gcttttcgtg cagctcatac attgaaaggg gtatgcatga atttaggctt tgatcatttg
     5281 tataaaccaa gttttgaaat cacagaaagt ttacgtgcag gaaacttgga attggcttta
     5341 caacaatttg atgcagtcaa agagcaatat actaaaacaa ttgatgcttt aaatgaattt
     5401 gcagcataga tctctaaaag aggtcttttt ttattgcttg taaataaatg taaaataatt
     5461 tcatttttat cttgcaagtt ttcagaaaat gaatataata atttacataa gcaaagaaga
     5521 gaagaagtac aaaagtaatt tctctaaaga gagataacgg aaggtgtaag ttattgtgaa
     5581 atcttttgga acgtgtcttg gagttatttg gtcgaagatt tgaggctgaa tcgtgaattc
     5641 gcgttaagaa tgaggtgtca tctgtatagg cagatggaat tttggtggta ccgcgtttat
     5701 tacgtccaat atatatttgg gcgttttttt attggaaagg gtgtgtgtga atggaaagtg
     5761 atcgacgata tgaaataatt tttgatttac acacgaagaa aggtaggaat aaaacatgaa
     5821 aaaaattatt aaattaggta tggcggcatt aaccgcatta acaatggcag ggtgttcaag
     5881 tgagtcagag gatgtaaaaa caattactgt ggggatttct ccagattatg ctccttatga
     5941 atctgaaaat aaaaaagggg atatcgtggg atttgatcca gatatgatga agtgttttga
     6001 agaatattta agtgaagaag aaggagttac ttataaatta gaaatgaaga agatggattt
     6061 tgataatatc attactcagc ttcaaggaga tcaaattgat gtaggcattt ctggatttac
     6121 atatgattca aaacgtaaag tggaatggtc agatccatat ttaggatcaa gtcaggtggc
     6181 tgttgttgtt aaagattcag atatcgcatc gacttctgat ttggaaggta aatctttagt
     6241 tgcccaaaca ggagctacag gtgagacggc tgcaaaagaa gttaaggatg ccaaagtaac
     6301 tggattaaag aatgtgcaag acattatgaa tgcattaagt gcaaatcaat atgatgcagc
     6361 aatcgttgat ttgggtgtag caaaaaacta tgtgaagagt ggcgaattta aaatgttaga
     6421 tgaatcttta atggatgaac aaaactatat tattgcgaaa aaaggaaata cagatgtgat
     6481 tgacaagatg aatacatgta ttaaaaaatt cttggctagt gaggattatg ctaagttgtg
     6541 caaaaaatat ggtttatctc cattagaaac aaagtaaagg aaaagtgagg aaagtgtatg
     6601 tttgttgcga ttgatcaggt atttacatta gagaatatac tctatttggc aaagggtgcg
     6661 ctgttgtcgg ttgcgattgc agcgctttcc cttttaattg gtttagtgct tggcatttta
     6721 ggagcaagtg gaagaagaag caaacataag gtgcctaggg ccatatcatt tgtgtatgtc
     6781 acaatcattc gtggaacacc attgcttcta cagattttaa ttatcttctc agttattcca
     6841 tctatctata cagcttttac aggtcatgta ttaagaatca atccaattgt gattggtatg
     6901 attgcgttaa gtattaactc cggcgcttat caaactgaac ttttgcgttc aggcattaat
     6961 ggagtagata aaggtcaatg ggaagcttgt gaaacattag gattatcgaa ttggaaaaca
     7021 atgaagttag taattttacc ccaagctttc aaacgtgttg taccaccatt gatcagtgaa
     7081 tttattactt tgattaaaga tagttctttg attagttgta ttggagcggt tgaactatta
     7141 aaaggagctc aagttattgg ggctgaatat tatgatgtaa tgtcgccata tatgcttgcg
     7201 gcaatattct atttgattat gacatgtatt atctcttgta ttggaaataa agtggaaaag
     7261 aggttggctg taagtgatta gagtagagaa tgtttcaaaa caatttaata aaacacgtgc
     7321 cctaaaaaat gtaagcttag aagtcaataa gggggatatt atctcgttga ttggtccaag
     7381 tggttcaggt aaaagtacat tattacgttg tattcatggc ctggagcatg tggattcagg
     7441 aaagatttat atggatgatg aatggatgaa tccggatgat gaaaagaaat ttcgggtgca
     7501 aagaaatcgt atgggatttg tgtttcaaca cttcaattta tttccgaata tgtcggtctt
     7561 acaaaattgt aagttagcac aagttgaggt gttgaataaa acggatgaag aagctgaaaa
     7621 aacagcttta gagtatttag ataaagttgg attattagaa aagaaagatg cgtatccaaa
     7681 taatttgtct ggtgggcaaa aacagcgtgt tgcgattgca agagcactat gtatgaatcc
     7741 agatattatg ttgtttgatg aaccaacaag tgctttggat cctgaaatga tcaaggaagt
     7801 acttgaagta atgaaagatc ttggcaagca aggtatgacg atggttgttg ttacacatga
     7861 aatgggtttt gccagaaagg tagggactcg agttgttttc ttggatcaag gagaaattat
     7921 agaggaaaac acaagcgaag agttttttgc gaatcctcag tcggatcgtg caaaagattt
     7981 tttaagtaag gtattctacg aatgagattg ccggatttta aagtcgaaca atggatgaat
     8041 gattatgaaa aggatgccat ctataatatg acggatacgt gtgtaaaagc gttaacttta
     8101 aaggaactat tagattgtga ggattttgat tttaatgaat tgactttgga ttatggtcaa
     8161 atcacaggga atgttgaatt aaaaaaagaa atcttatctt tatatgcgag tggaacgatt
     8221 gataatatca caacggcaca aggttgcctg caggccaatg aattagtgat gaatacattg
     8281 ttggaaaaag gggatgaagt gatttgtgtt gttccagggt atcagcaatt tgtagatatt
     8341 ccaaaatcaa ttggatgcaa ggtgcgttta attgagcttg atgaatatga ttggcaggtt
     8401 ggtgtaaaaa agtttagaaa gatgctgaat gcctcaacaa aaatgattat tttaaataat
     8461 ccatcgaatc caacgggaac agaatattct atcgattttc taaatgaatt gattgaagta
     8521 tgcaaaccat atggtactta tattctttgt gatgaggtat atcgtggttt aaatcaggaa
     8581 gtctcaatta gtgatatata tgaaaatgga atttcgacat cgagcttaag taaagtgttt
     8641 tcgcttgctg gattacgcct tggctggatc aaagccaatg agtatgtgat tcatcaaatt
     8701 aatgtgagaa gagattattc tatgatttct acaggtcctt tagtggataa attgggatgg
     8761 attgccctta agcataagga tgaattgatt ttaagggcca agaaaattat ttcatctaat
     8821 aaaagtattg tatgtgaatg gcttaaagaa aatcctcgct ttgaatgtgt attgcctagt
     8881 ggtggaacag tgtgtttttt aaagtattat tttgatttaa agagtgagac attttcgaaa
     8941 ttattattgg cagaaaaagg tgtatttttt gtgccaggct cgtgttttga taaagaatat
     9001 tattttcgtt taggcttggc tcaagatagt aagctattta agtctggctt ggatgaactt
     9061 tcgaactttg tggatgaaca tttgatttca tgataaaata atttgcgtaa gggggaatac
     9121 ttatgcgaat tcaatatttg ttgtgtataa gcctattact ttgtgggtgt acaaaagtga
     9181 gtgatcttac gcatcaaaaa agtaagtcaa cagaaaaggc gcctgtaaca gtaaaaaaga
     9241 caaataaagt aaaggtgatt gattgtaaaa atgattcttc acagcaggtt acttttgaag
     9301 caaaagggga taaaattcaa aagatgacac aaaaatttac aatgagtttt tcggatttgg
     9361 ggattacgga agatatgaat tctgaacaga ttcaatctaa aatcaactct tcattagatg
     9421 ataaatacaa ttcgattgaa ggggtacatg tttcgacgca attaaaggac aaacaggttg
     9481 aagtgacgtt ggaaatggat tttaaaacgg caaatataga tatgttgatt gaaaataatt
     9541 tgttggataa aggggaagcc caatcggatt atgtaagttt aaagaaaacg caaaatgctt
     9601 ttaaaaaaga agggtttgcg tgtataacaa aatagcggaa tttccgctat tttctagttt
     9661 gaaaggatgc gtaaaaagat gcgttattat attgcggaca cacatttttt tcatcgttca
     9721 ttaaatgaaa aaatggatca aagaggcttt gacgatattg atcaaatgaa tgaatatatg
     9781 attgatcagt ggaatagtaa agtgcgtaaa aatgatgagg ttgttgtgct tggcgatttt
     9841 tcatggggaa atgcaaagga aacaacagaa attttggatg aattaaaggg taaaatttat
     9901 ttgattcgcg gaaatcatga tcgttttcta gatgacaaaa attttgatac ttcaagattt
     9961 gtttggatca aagattatgc ggaattaaat gagaataaac gaaaagtcgt attatctcat
    10021 tatccaattg cttgttatga tgggcaatat agaagggatg agtttgggaa tcctaaaact
    10081 tttatgttgc atggacatat tcatgcgacg caagatcagc agtttttgga tgcttatcaa
    10141 gattatgttc ggcaacaaaa acatccaaga atttcagatg gccaattaga aaatgtgcct
    10201 tgccaactta ttaattgttt ttgtatgtat agcgattacg tgccaatgac attagatgag
    10261 tggattgaaa ttgacaagca aagaagaagt gtcttataat tgttataaca aatatataag
    10321 gagatcatgt tatgcacaaa ttggatacaa cgccaggtca tcttccttac tataaacaaa
    10381 ttaaggaatt ctacaaaaaa gatattcaag aaggagtatt ggctgtagga gatcgcgttg
    10441 atagtgaaat ggaaattcaa gagatgtacg atgtttcaag aattacggca agacaagcta
    10501 ttcttgaatt agaacaggaa ggtatggtaa agcgtggccg tggcaagggt acgtttgtga
    10561 catatagacc taaaatcaaa gaagaattgt cgcatattcg tagttttaca gatgaaatga
    10621 cagctttagg aagaacacct ggaactaagt cttttcacat cacaaaagaa atcccggatc
    10681 aagccacaag agaattgttt ggcttggacg aagaagtgat gtattgtgtt cgaagagttc
    10741 gtactgcaga tgatgtttta ttggtatatt ttgtttcgta ttttccatta gacttaaata
    10801 ttcctttaaa tatggaagaa caaacacagt ctatttatga agtgattggc gcaccgacaa
    10861 gaatagatga agagttttcg gcaattaatc cgagtgaaga aatatgcttg gcattaaaaa
    10921 tacgtaaaga tcagccggtt ttagctcgta aacgtgtttc ttatgataaa aagaaaaaaa
    10981 agatggagta tacaatgtgt tactatcgtg gcgatttata tagttacaca atttcaacgt
    11041 cgcaaaaatt atgattttaa cgatcgacat tggtggtacc aatattaaat atggattatg
    11101 cgataagtca gggaatatcc aacaaaaagg agaatatcct actttggata cggctgataa
    11161 aattgtgcaa tccatttgtg atttaccatt tgaatatact ggaattgcga ttagtatgcc
    11221 cggaattgtg aatgaagatc attcaaaagc atttattacg ggtaagcttt cgtttctgtc
    11281 gaaccaccct ttaaaaaagc gattggaaga aattaaaggt tgtaaagtga caattgaaaa
    11341 tgatggtcgt tgtgcgacgt gggctgaact tggatatggt aatttaataa attcaaaaaa
    11401 tgcacttgtt gtggttctag gtacatatat tggaatgggg attgtcattg atcataaggt
    11461 atatgaaggt tcacatttat tggcaggtga atattcggat gcgcatatgg atacggaaaa
    11521 gacttttaag aatacgatgg cggcctactt tggaaaagat ggtcttgaaa gagcaacggg
    11581 tttaacgggc aaagaacttt ttgatcattt agatgatcaa aaagtgcatg aaggattgaa
    11641 tcagtattgc caaaatttag cttggatgct aagtaatatc cagctacttc tagatgtaga
    11701 taccattctg attggcggag gtatatcggc acaaccaaga ttaattgagt gtatcaaaga
    11761 aagtatgaat aatgtcaaat tgtggttttg tacattaaaa atgcctgaga tacaggcatg
    11821 taaatttaac aatgacgcaa atttattggg tgcgttatat tattataata ataccgagaa
    11881 acattaaacg ttctcggtat ttcttacatt gagggggaag gacatgaaat ctttaattgg
    11941 tgataagaag ttttatcgaa tggttttgat tgtatgtata ccgattatga tacagaatgg
    12001 ttttaccaat cttgccagtt tattagataa cattatgatt ggacaattag gaacactatc
    12061 tatgagtggt gtatctattt caaatcagtt atttcaagta tttaatgtga gtgtttttgg
    12121 cgcaatgtca ggggctggaa tatttttggc acaattttat ggacgtggaa acagggatgg
    12181 tgtacacaat tgtttccgaa ttaaaatgtt gctttgtatc ttgatttcaa tattggccat
    12241 atgcattttt aaattgttgg ggccttcctt aatttcttta tatttaaatg atgatccgac
    12301 agatagtatg acaacattga gctttggcaa gcaatatatg gatgttatgt tgattggact
    12361 cattcctttt gcgattacgc aagtatattc ttcttcatta agagaaacag gaaatacagt
    12421 tttaccaatg aaggctagtg tcatagcggt tattgtgaat ttttgcatca actatatttt
    12481 gatttttggc cactttggct ttcctaaact tggcgttatt ggtgcggcca ttggtacggt
    12541 tgtttcaaga attgtagaga tggggataaa tatagcggca ggcttaaata acaattattt
    12601 aagggatgcg atgcgtttat ataagatatc gggtgatata tttaagaatg ttgttaaaag
    12661 aggaatgcct ctattatgta atgaaatatt gtggtcgata tccattgcgc ttatttctca
    12721 atgttattca acaagaggac tttttgcagt tgcagccatt aatgttacaa caacagtaac
    12781 caatttcttt atgattattt gttatgcaat gggaaattcg atttcgatta ttgtaggtca
    12841 aagactgggt gctggtgaaa tagagtatgc taaagattgt gatttgaaga tggtgtttat
    12901 gaattttatg atgtgtttat gcattggtgt ggcattgttt ggatgttcat cttttattcc
    12961 tcaaatctat aatgtgtcta tagaagtaaa gtcattagca acatctttat taagagtttc
    13021 ggcttgtatg cttccaatca tttctttgta ctatagcagt tactttacaa tgcgtgcagg
    13081 agggaaaaca ttactcactt tcttcttcga tagtggatat acatttatat ttacatttat
    13141 ggttgcgcta tgtttaactc gttttacgaa tttaccaata ttcaatgtgt acttattggt
    13201 gcaatgtgtg gatattccta aggcagtatt aggaatcaca ttggttaaaa aaggaatatg
    13261 ggtacataat attgtcaatg aactttagcc ttctttttag aaggcttttt cttttagaag
    13321 gcaaaaaaaa gaagtccttt cggactttgt ttgaaaaatg gtgacgcgta cgggattcga
    13381 acccgtgagt gcatgcgtga aaggcatgtg agttaaccgt ttctccaacg cgccatgtat
    13441 attaaacttg tcttccttga caagtgctag ttaatgatac tataagttta tattaattgc
    13501 aagcaaaaaa ataaaaaaat caaaaatatg gtcaaaaata ttttttgcat ttcttaaatg
    13561 agtcataata ataagatgaa attatttgtt aacatagtga ttataaagct tttaaatagg
    13621 cagtggtagt aacgatttgt gcatatgatt ggtagatatt atcaaagtta cagagtgatt
    13681 ttgtagacaa taatacatac gaatttgata ggaatatagt tatattttat acatgataat
    13741 aatatttgaa tctgaataaa aatagtgatt atacaaataa
//
LOCUS       DBMDWL010000040        13550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1540_length_13550_cov_3.409845, whole genome shotgun sequence.
ACCESSION   DBMDWL010000040 DBMDWL010000000
VERSION     DBMDWL010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1540_length_13550_cov_3.409845"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="ACZPDN_04275"
     CDS             <1..581
                     /locus_tag="ACZPDN_04275"
                     /inference="COORDINATES: protein motif:HMM:NF028352.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HIR1297870.1"
                     /translation="LFHSDYLFSGGSGLLEHLFQFHNNEHVFSAKIATKTIVLCGSCS
                     KATKSQIETWINKGKTSYAINPNSNIEELYSYIQESESEILFYSDAVFRDLTNTSRDS
                     TFYSNAKKIESILSKLGICAYKNNFDRLIVAGGETSGAITSALNFTSFYIGKEIAPGV
                     PVLIPTNQPNFHLILKSGNFGNKEFFLEALED"
     gene            585..1151
                     /locus_tag="ACZPDN_04280"
     CDS             585..1151
                     /locus_tag="ACZPDN_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1297871.1"
                     /translation="MDDVLKSKYEEAIWIAKTLFQHGIITGTTGNISFLHNEKMYITK
                     SGSCLGKLDKDGFAITDLNGNILFNKPSKEYIMHLALYNSNETYKAIIHTHSFYTTLL
                     SYNNHSYIDTPYMKLKNEKLKYVDYFPPGSEELFSAVENSLEENNDTYILEKHGIICA
                     KDTLLSAFGTIEEIEYSAKLSFLLTQEN"
     gene            complement(1276..2016)
                     /locus_tag="ACZPDN_04285"
                     /pseudo
     CDS             complement(1276..2016)
                     /locus_tag="ACZPDN_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            2353..4185
                     /locus_tag="ACZPDN_04290"
     CDS             2353..4185
                     /locus_tag="ACZPDN_04290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HIR1297872.1"
                     /translation="MNYQQLGNDIIQSIGGIDNIKQLTHCATRLRFEFYDMNKVNTKK
                     VESLNGVISVVEKGGQYQVVIGNEVQSVFRAIQSQMKDVPSTSHEESDEEKGSIVNRI
                     ISVISTTFTPVIPALIGGGMIKAVLSILVLFNLVNEASSTYEIINFISDAPFYFMPVL
                     LAYGASQKFKTSPIMAMTMACALLHPTWSGLVAAGSPLTFFGAPVVLVDYASSVIPII
                     LSVWIMSYVEKFAEKYSPSIIKFFLKPLLVMFISVPIALIIVGPLGNLLNDLVQSGAN
                     YLNAHVSWLIPMLMGAFQPFLTLTGTAWAMTPIATSQISTLGYEIVNGPGMLASNIAQ
                     GGATLAVALKAKDENLKSLASSSGFTAVMGITEPCLYGVLLKLKRPFVASMIGGAAGG
                     IYAGITGLVRYAFVSPGLTAIPAFIGKNPMNVVHAIVTMIISFVVGFVASWILGFKED
                     APQEEVVATVSKDEIVAPCDGTVKPLSEVNDDVFSQEIMGKGIAIEPSTGNLYAPCDG
                     TITVCFPTGHAIGLKSKNGTEILMHVGIDTVNLQGKYFSPQIQVNEEVKKGQLLLTFD
                     LAKIKEEGYDTTTPIIITNSKNFSSLQFENDTTIQHGNKLIFLA"
     gene            4196..5608
                     /locus_tag="ACZPDN_04295"
     CDS             4196..5608
                     /locus_tag="ACZPDN_04295"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR1297873.1"
                     /translation="MHYSKLKDFPQNFLWGASTSAYQVEGAIDEDGKSPSIIDMYEHP
                     SDVADFSVASDHYHRYKEDIALFAEMGLKAYRFSIAWTRILPNGTGKVNEKGIEFYNN
                     VIDECLKYNIEPVVTMYHFDLPYCLEEKGGWLNRDTIEAFVEYAKVLFIHYGSKVKYW
                     LTINEQNTMILHPGAIGLPKGGKLPSKKDLYQQNHHMMLAQAKVMKLFHNMVPEGKIG
                     PALNLTAMYPATCNPNDAIAAHNWEVLRCWNFVDVCAFGNYHPLAWSYLKDRNIAPEI
                     QDGDFETLKGANPDFIAMNYYSTATIAASKGDASDVAARAGDQQIMLGEQGVYRPSEN
                     PYVEKTQYGWVVDPVGFRYTLRKVYERYQLPILITENGIGANDTLGPDGCIHDQYRID
                     FIQKHLHQMRLAITDGVKMLGYCPWAAIDVVSTHQGYKKRYGFIYVDRDEFDLKELKR
                     YKKDSFYWYKQVIEENGKNI"
     gene            5627..6463
                     /locus_tag="ACZPDN_04300"
     CDS             5627..6463
                     /locus_tag="ACZPDN_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIR1297874.1"
                     /translation="MIIEKFLNNNMLLVNDNHEEMIVMGKGLRFHYKVGDEIRDEDIE
                     KTYVLQDSTNKNGYLQVLENAPDIYLEIVHHIIEMAQLNISSPFNKQIFVTLLDHLLY
                     AVERYEKGIVLQNRMLWEIQKFYPNEFMLGLQAVDYINETLHIQLPETEAGNIAFHFV
                     NAQIKSNDLSTENGMLMVKMLKDIFQIIQLHYGYTIDENSLNYSRFVVHMQFFLQRLL
                     ENKMLENENDDIYYTYIIKNSSALDCVEQIEKYIDRLLHKQITRDEKIYLIVHITRIT
                     TH"
     gene            6584..8455
                     /gene="ftsH"
                     /locus_tag="ACZPDN_04305"
     CDS             6584..8455
                     /gene="ftsH"
                     /locus_tag="ACZPDN_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1297875.1"
                     /translation="MNKQKKPLYRYYTIILIVIMALNFIIFPMFQQSKLETTNYSDFL
                     NKIEEKKIDTVQIEDHNIYYTLKKDSTDKTYKTGVMNDSDLVNRLDKSGAKFGQVYQE
                     QMNPILSSLISFFLPLIIFWAFGSWMFKRMQKKMGGDDQMSFSQGSGFGLGNLGKSNA
                     KVYVGEQTGKTFNDVAGQEEAKENLQEIVDFLNNPAKYKEIGAKMPKGALLVGPPGTG
                     KTLLAKAVAGEAGVPFFSISGSEFVEMFVGRGAAKVRDLFKQAREKAPCIVFIDEIDT
                     IGKKRDGAGMNGNDEREQTLNQLLAEMDGFDGSKGVVLLAATNRPDSLDPALTRPGRF
                     DRRIPVELPDLAGREAILKLHAKDIKCSSNIDFNVIARASAGASGAELANIINEGALL
                     AVRDHRKTVVQKDLEEAIEIVIAGEQKKGAVISNRERMIVSYHEIGHALVAAKCKNTA
                     PVHKITIIPRTKGALGYTMQVEENEQNLLSKEEAFQKIMVYTGGRCAEELIFNSITSG
                     ASNDIEQATKLARAMITRLGMSDEFGMTALETVNNQYLGGDTSLACSNETATKIDEAT
                     IALIKKAHDEAYQILEDNKMKLHELAKFLYERETITGEEFMYILNKPAEIPAQTNKD"
     gene            8604..9905
                     /locus_tag="ACZPDN_04310"
     CDS             8604..9905
                     /locus_tag="ACZPDN_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297876.1"
                     /translation="MEIQRNLYLQKLIACQHDGQIKVITGLRRCGKSFLLFHLFKNYL
                     LDNGIDNQQIITFELDQMKDIQYRNPIELSKKIHSIVDNTTQQYYLFIDEVQMSDEVN
                     NPYNPKGKKISFYDMLNDLKELSNLDIYVTGSNSKMLSNDILTEFRGRSDEIKIHPFS
                     FSEYYSFVGGDKEEAFDNYSFYGGMPFLLTKQDETSKIEYLENLFKEVYIRDIVERKH
                     IEREDILSSIIDLLCSSVGSLTNPSKITKAINSKQQRSGKDIVYLPTINKYIDFLEDS
                     FLFKEALRFDVKGKSYLDFPKKYYCEDIGLRNARTGFRQIEMPHIMENILYNELIIRG
                     FKIDVGVVYENIKTEKGNYRKVAREIDFIIQKGMKKYYIQSAYTMENEEKVYSESRSL
                     NITGDSFPKIIVRRDIRKRFYDENGILNIGLIDFLLSDNVL"
     gene            complement(10165..13368)
                     /locus_tag="ACZPDN_04315"
     CDS             complement(10165..13368)
                     /locus_tag="ACZPDN_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1297877.1"
                     /translation="MNQLLQEKRDAENFLRLIAPKYAAVYILDRSTDTFRDILAPEVF
                     RAFAKDTKGSYSDAMQLYRDHYVACDYQEVIDRVLDYDYVYNILASGNQVNVTYRKKN
                     GTLIRLKIDRYSDNDENTSIWVYTNEDSEDALYGELGEARYRVLFDDHERPVEFIGSE
                     SLSEMLYGFKGETHISFSMLWNHMHPQDIDVVKEEFKNIHDTRDSTVTYEVEYRFQNK
                     DKQYRWYRAIGKPVLNERGKIVSVCGFIIDIHKHKEKNLLQQRFISGLSHEYVTVWVI
                     NHDLTVELYQQTKKGDHIAQEAIEKFAKENNYQKSMEKYASEYVCEEDREDFLRNVNY
                     RVVRERIKTEKVYTVVFQRKFNGKIDYFQTCFAQISNETDDFVLGFKLVNDIIQQEKE
                     RNDRLEEEKQILESLSSDFTAIYYLEVNSGKFEPVHIQDETNASELIRSYSGYKDFYE
                     YAYQYAMRYIPKEEQPEFLWWFSRDNLKSLLKTQVSLTQNYRCYPNAQNQQYFETKVV
                     KVSEDEESCHALLGFRYIDDIIKKEKATQKRLQNALEEIKRSNETISTIAKSYSSIYK
                     VDFETDTYERISGSDIIPKTGCASEKMFDVCEQEVAPEYRNIIHQFANIETLTSRFEK
                     EEDVLAEYRMVDGNWHKLRIIVSKRNANGKAIQAIATIRSISETKRKELNLFFEAEQA
                     KREAKIKTRFLQSMSHDMRTPLNGIAGMLHMADQNPKDLELQKITRNKIHRSLNYLIS
                     LVNDVLDMNDLESDHFTEEEVDFDVTKLLESINVDGQQLAQEKNIQYILDWYEGQLSH
                     SRFRGYPIYLSRILLIVVQNAVKFTPENGEIHVWMNEKCLDESTSLLEFRCKDTGIGM
                     SQDFIKHAFDLFAQEDSSSRSTYQGTGLGLPIAKKLVEYMHGNIQLESEKGVGTTVSI
                     QIPFKLGKPIENKNNRHQQISLEGLCVLVAEDNDLNMEIVEYMLERNGIRVVCAKDGQ
                     EVIDLFEKSEENKFDFILMDIMMPKINGLDATRKIRALKRLDAKTIPIIAMSANAFVE
                     DIMNSKIAGMNMHLAKPLDETKLINALKQCKKDERVN"
BASE COUNT         4586 a         2441 c         2140 g         4383 t
ORIGIN      
        1 agttgttcca tagcgattat ctttttagcg gtggaagtgg tttactcgaa cacttattcc
       61 aatttcacaa taatgaacat gttttttctg caaaaattgc tacaaaaaca attgtattat
      121 gtggaagttg ttctaaagct acaaaatcac aaattgaaac atggataaat aaaggcaaaa
      181 cttcctacgc tatcaaccca aattctaata ttgaagagct ttattcttac attcaagaat
      241 cggaaagtga aattctattt tatagtgatg ctgtctttag agatttaacc aacacaagca
      301 gagactctac attctactcg aacgcaaaaa aaatagaatc cattctctca aaattaggta
      361 tctgtgctta taaaaataac tttgatcgac ttatcgttgc aggcggagag acatccggag
      421 ctattacatc tgcactaaat ttcacttcat tttacattgg aaaagaaatt gcacctggcg
      481 ttcctgtatt aattccaaca aatcaaccga attttcattt aattttgaaa tcaggaaatt
      541 ttggaaataa agaattcttt ttagaggcct tggaggatta aaatatggac gatgtattaa
      601 aatcaaaata tgaagaagct atttggattg caaaaacatt atttcagcat ggaatcatta
      661 ccggtactac aggtaatatt agctttcttc ataatgaaaa aatgtatatc acaaaaagtg
      721 gtagttgtct tggcaaatta gataaggatg gcttcgccat aactgattta aatggaaata
      781 tattatttaa taaacctagc aaagaatata ttatgcattt agctttatat aattcaaatg
      841 aaacatataa agcaatcatt catacacata gtttttatac tactttatta agttataata
      901 atcattcata tattgacaca ccttatatga agctcaagaa tgaaaaatta aaatatgtag
      961 attattttcc acctggatca gaagaattat tctcagccgt tgaaaactcc ctagaagaaa
     1021 acaacgatac atatatttta gaaaaacatg gaatcatttg tgctaaagat actttattat
     1081 ctgcctttgg aaccatcgaa gaaatagaat atagtgctaa actatctttt ctattaactc
     1141 aagaaaatta aaacaggtcg ccttataggt tagacctgtt tttactttat ctagataaat
     1201 ttgtattctt agaattcatg attttaaata catcttcttg tgttaccaca ttaaaatcat
     1261 gttgaatcat aagctttaat acactcgcac tgattgcgaa atcgactgtt ttctgtggat
     1321 caaaccatgc acaaatgcag caccataggc atctcctgct cctacagtat gcatattttt
     1381 taatacactt gtcacactct ttacatttgg aaattgacga gtaatttcta acataccttc
     1441 tttataagta tcaatctgat caatacctgt agataagtct ccatcaaaag catggatacc
     1501 tagtgttgct tcaaaatctt catcattggc aatacataca tctacatact gcattaaatc
     1561 acgcatgacg acttgcgctt gttctttaga ccacattttt ccacgatagt tcaaatcaca
     1621 aatcacttga atatcatttt ccttacaata tttcaaagca cttcttactg tatcctcaac
     1681 atatttagat acagctggag ttacacctga aaaatagaaa acatctcctg gctctaagat
     1741 attttcccaa tgatattcac ttggctgaga tagtgaaaaa gcactaaatg cacggtcata
     1801 tacaacgttt gtattacgaa tatcacttcc tttttcaaag aagtaaattc ctaaacgatc
     1861 gcctccacga agcatatact ctgtatttac accataatga cgaacttcac tcaacgcaga
     1921 cattcctact ggattgtttg gtacttttga tacaaaagca catttatctt catacatcgc
     1981 taaacttaca gctacattga cctccgcgcc accataactc gcctcaaaac catcgcttta
     2041 cataatacgc aagttttcag gtgtttttaa acgaagcatg atttctccaa aagttactac
     2101 cttcataata tatatcctcc ataaacgctt taattatacc attttttaat gtctactcat
     2161 ccttattttt tgtcttttca tgaaagggat ttcaaaaaat gcttgtattt tatttttagc
     2221 tatgctatca tcctacatgt aaataacggc tgttactggt aatgcaggca agaccaagat
     2281 atttaagttg catttttaat gctcctttta tcttggtctt ttttattgcc atcagcctag
     2341 aaagaggaga atatgaatta tcaacaatta ggaaatgata tcatccaaag cattggtggt
     2401 atcgacaaca tcaaacaatt aacacattgt gcaactcgtt taagattcga attttatgac
     2461 atgaataaag tcaacaccaa aaaagtggaa tctttaaacg gcgttatcag cgttgtagaa
     2521 aaaggtggtc aataccaggt cgtaattggc aatgaagtac aatccgtttt tagagccatt
     2581 caatcccaaa tgaaagatgt tccttctact tctcacgaag aatccgatga agaaaaaggc
     2641 agtattgtca atcgtattat cagtgtaatt tccactacat ttactcctgt gattccagcc
     2701 ctaatcggcg gtggtatgat caaagccgta ttatctattc ttgttttatt taacttagtt
     2761 aatgaagcaa gttccactta tgaaatcatc aactttattt ccgatgctcc attctatttc
     2821 atgcctgtcc ttttagcgta tggagctagc caaaaattca aaacaagtcc aatcatggca
     2881 atgactatgg catgtgctct tttacaccca acatggtcag gcttagtcgc tgcaggaagt
     2941 ccattaacat tctttggagc tcctgttgta ttagttgact atgcatcaag tgtaattcct
     3001 attattttaa gcgtgtggat catgtcttac gttgaaaaat ttgccgaaaa atactcacca
     3061 tctattatca agtttttctt aaaaccactt ttagtgatgt ttatctctgt accaatcgca
     3121 ttaatcattg ttggtccatt aggaaactta ttaaacgatt tagtacaatc tggcgctaac
     3181 tatttaaatg cacatgtaag ttggttgatt ccaatgttga tgggagcctt ccaaccattc
     3241 ttaacactaa caggtactgc atgggccatg actccaattg cgacaagtca aatttctaca
     3301 cttggatatg aaatcgtaaa tggacctggt atgttagcaa gcaatattgc acaaggtggt
     3361 gctactttgg cggttgctct aaaggcaaaa gatgaaaact taaaatctct tgcatcttca
     3421 tctggattta cagcagttat gggtattact gaaccatgtt tatatggtgt cctattaaaa
     3481 ttaaaacgcc catttgttgc ttctatgatt ggtggagctg ccggaggtat ctatgcagga
     3541 attaccggcc tagttcgtta tgcctttgtt tcaccaggat taactgctat tccagccttc
     3601 atcggtaaaa accctatgaa cgtagttcat gccattgtca caatgattat ttctttcgtt
     3661 gtaggttttg tcgcatcatg gattttagga tttaaagaag atgcaccaca agaagaggta
     3721 gtagctactg tttcaaaaga tgaaattgtc gcaccttgtg atggaactgt aaaaccacta
     3781 tccgaagtaa atgacgatgt cttctcacaa gaaatcatgg gaaaaggtat cgcaatcgaa
     3841 ccaagcacag gaaatttata tgctccttgt gatggaacaa ttactgtatg tttcccaacg
     3901 ggtcatgcaa ttggattaaa atcaaagaat ggaacagaaa ttttaatgca cgtaggtatt
     3961 gatactgtta atctacaagg aaaatatttc agtcctcaaa ttcaagtcaa tgaagaagtt
     4021 aaaaagggac agttactatt aacttttgat ttagccaaaa tcaaagaaga aggttatgac
     4081 acaaccactc caatcatcat cacaaattca aagaatttca gttcccttca attcgaaaat
     4141 gatacaacca ttcaacatgg aaacaaatta atttttttag cttaggaggt aaaccatgca
     4201 ctattctaaa ttaaaagact tccctcaaaa ctttttgtgg ggagcatcta cctcagcata
     4261 ccaagttgaa ggtgccattg atgaagatgg taaaagccca tctatcattg acatgtatga
     4321 acacccaagt gatgttgctg atttctctgt agcttctgat cattatcata gatataaaga
     4381 agatattgcc ctatttgcag aaatgggatt aaaagcttat cgtttctcta tcgcatggac
     4441 tcgtatctta ccaaatggaa cgggtaaagt gaatgaaaaa ggcatcgaat tctacaacaa
     4501 tgtcattgat gaatgtctaa aatacaacat cgaacctgtt gttacaatgt atcactttga
     4561 tttaccatat tgcctagaag aaaagggtgg ttggttaaac cgtgatacga ttgaagcctt
     4621 tgttgaatat gccaaagtat tatttatcca ctatggatca aaagtaaagt actggttaac
     4681 gatcaacgaa caaaatacga tgattctaca tcctggtgca attggattac ctaaaggcgg
     4741 aaaattacca tctaaaaaag atttgtatca acaaaaccac catatgatgc ttgctcaggc
     4801 aaaagtcatg aaactcttcc ataatatggt tccagaagga aaaatcggac ctgcactaaa
     4861 tttaacagct atgtatccag ctacttgtaa tccaaatgat gcgattgctg ctcacaactg
     4921 ggaagtactt cgctgttgga actttgtcga tgtttgtgca tttggaaact accacccttt
     4981 ggcatggtct tacctaaaag atcgtaatat cgctccagaa attcaagatg gagactttga
     5041 aacattaaaa ggcgcaaacc cagactttat cgccatgaat tattattcaa ccgcaactat
     5101 agctgcaagt aaaggagatg cttcagatgt agcggcaaga gccggtgacc aacagattat
     5161 gctaggagaa caaggcgtat atcgacctag tgagaaccca tacgtagaaa aaacacagta
     5221 tggatgggtt gttgatcctg ttggattccg atatacttta agaaaggtat atgaacgtta
     5281 ccaacttcct attctgatta cagaaaatgg aattggtgcc aatgacacat taggaccaga
     5341 tggatgtatt catgaccaat atcgtattga ttttattcaa aaacaccttc accaaatgcg
     5401 cttagccatt acagatggtg tcaaaatgct tggctattgt ccttgggctg ccattgatgt
     5461 tgttagtaca catcaaggct ataagaaaag atacggattc atttatgtcg atcgtgatga
     5521 atttgattta aaagaattaa aacgatataa aaaagacagt ttctactggt acaaacaagt
     5581 aattgaggaa aacggaaaga atatctaagt agatgaggtg cttcacatga ttatcgaaaa
     5641 atttttaaac aacaatatgc ttctagtgaa tgataatcat gaggaaatga ttgtgatggg
     5701 aaaggggctg cgttttcact ataaagtagg agacgaaatt agagatgaag atatagaaaa
     5761 aacatatgtt cttcaagata gtacaaataa aaatggatat cttcaagttt tagaaaacgc
     5821 acccgacatc tatttagaaa ttgtgcacca catcattgaa atggcacaac ttaacatttc
     5881 ttctccattc aacaaacaaa tctttgtgac cttgttagat cacttgttat atgcggtaga
     5941 aagatatgaa aaaggcattg ttctacaaaa tagaatgttg tgggaaatcc aaaaatttta
     6001 tccaaatgaa tttatgcttg gtttacaagc tgtagactac attaatgaaa cacttcatat
     6061 tcaattgcct gaaacagaag ccggaaatat tgcctttcac tttgtgaatg cacaaatcaa
     6121 atcgaacgat ctttcaacag aaaatggtat gttgatggtc aaaatgttaa aagatatttt
     6181 tcaaatcatt caattacatt atggctatac aattgatgag aattcgctaa actattcaag
     6241 atttgttgtt cacatgcaat ttttcttaca acgactttta gaaaataaaa tgcttgaaaa
     6301 tgaaaatgat gatatatatt atacatatat tatcaagaat tcttctgcat tagactgcgt
     6361 agaacaaatc gaaaaatata tcgatagatt actacataaa caaattacgc gtgatgaaaa
     6421 aatatattta attgtgcata ttacgcgtat cacaactcat taagtatcgc aatggcggta
     6481 ctttttcttt taaattatgt ggaattatgg aacgtgtagc actccacttg cgaaagtgct
     6541 aataaggtgt ataatgtaat caatttctta aggaggaaca ctcatgaaca aacaaaagaa
     6601 gcctttatac agatattaca caattatatt aattgttatt atggctttaa actttatcat
     6661 attccctatg tttcaacaat ctaagcttga aacaaccaat tactccgatt tcttaaataa
     6721 gattgaagaa aagaagattg ataccgttca aattgaagat cacaatattt attacacttt
     6781 aaagaaagac tcaacagata aaacctataa aacaggtgtt atgaatgatt cagatttagt
     6841 caatcgtttg gataaatcag gagctaaatt tggacaagtc tatcaagaac aaatgaatcc
     6901 tattctatcg agtctaattt cctttttctt accattaatc attttctggg catttggatc
     6961 atggatgttt aagcgtatgc aaaagaaaat gggcggagat gatcaaatgt cattttcaca
     7021 aggctctggt tttggcctag gcaatctagg aaagagcaat gccaaagtct atgtcggtga
     7081 acaaacggga aaaacattca atgatgttgc aggacaagaa gaagcgaagg aaaatctaca
     7141 agaaattgta gacttcttaa acaatccagc taaatataaa gagattggtg ccaaaatgcc
     7201 aaaaggagcc ttgttagtag gtcctcctgg aactggtaaa acattacttg ccaaagccgt
     7261 tgcgggtgag gctggcgttc cattcttctc gatttcaggt agtgaattcg tagaaatgtt
     7321 tgtcggtaga ggagctgcca aagtgcgtga cctattcaaa caagctcgtg aaaaagctcc
     7381 atgtattgta tttattgatg aaattgatac cattggtaaa aaacgtgatg gtgcaggcat
     7441 gaatggtaat gatgagcgtg aacaaacatt aaaccaattg cttgccgaaa tggatggatt
     7501 tgatggttcc aaaggcgttg tattgcttgc cgctacgaac cgtcctgatt cactagatcc
     7561 tgctttaacg cgtcctggtc gatttgacag aagaattcca gttgaacttc ctgatcttgc
     7621 cggtcgtgaa gccattctta aattacatgc caaagacatc aaatgctcaa gcaacattga
     7681 ctttaacgtg atcgctagag ctagtgcagg agctagtggt gccgaacttg ccaacatcat
     7741 taatgaagga gctttattag cggttcgtga ccatcgtaaa accgtggttc aaaaagactt
     7801 agaagaagcc attgaaatcg ttattgccgg tgaacaaaag aagggtgcag tgatttcaaa
     7861 tcgagaacga atgattgttt cttatcatga aataggacat gcactagtgg ctgctaaatg
     7921 taaaaacacg gctcccgttc ataaaatcac aattatccct cgtacaaaag gggctttggg
     7981 atatactatg caagtagaag aaaacgaaca aaacttatta tcaaaagaag aagctttcca
     8041 aaaaatcatg gtgtatacag gtggtcgttg tgctgaagag ttaattttta attccatcac
     8101 aagtggtgcg agcaacgata ttgaacaagc cacgaaactt gccagagcca tgattacacg
     8161 actcggaatg tcggatgaat ttggtatgac agctttagaa acagtaaaca accaatattt
     8221 gggtggtgat acatctttag cttgttcaaa tgaaacagca actaagattg atgaggcaac
     8281 catcgcatta attaaaaaag cgcatgatga ggcttatcaa attctagaag acaacaagat
     8341 gaagttacat gaacttgcca aattcttata tgaaagagaa acaattacag gtgaagaatt
     8401 tatgtatatc ttaaataagc cagctgaaat cccagcacaa acaaataaag attaagatgt
     8461 gtgtaatgca catctttttt aaatataatg tattgcgaat ttttgcatta cattatattt
     8521 cgtttgataa aagttttatc aaattgtata attttaatat catgtattgc gagttttgat
     8581 attactttat actaggagga tttatggaaa ttcaaagaaa tctatattta caaaaattga
     8641 ttgcttgtca acatgacgga caaatcaaag tcatcacagg cctgagaaga tgcggaaaat
     8701 cgtttctact tttccatctt tttaaaaatt atctattaga taacggcatt gataaccaac
     8761 aaattatcac ttttgaattg gatcagatga aagacattca atatcgaaac cccattgaac
     8821 tttcaaagaa aatccatagt attgttgaca atacgacaca acaatattat ctattcattg
     8881 atgaagtaca aatgtccgat gaagtaaata atccttataa tccaaaagga aaaaaaattt
     8941 ctttctatga tatgttaaac gacttaaaag aactatcgaa tttggatatt tatgtaactg
     9001 gcagcaattc taaaatgctt tctaatgata tcttaacgga gtttagaggt cgtagtgatg
     9061 aaataaaaat tcatcctttc tccttttcgg aatattattc atttgttggt ggagataaag
     9121 aggaggcatt tgataattat tcgttttatg gtggaatgcc ttttctactc acaaaacaag
     9181 acgaaacctc caagattgaa tatctggaaa acttatttaa agaagtgtat attcgagata
     9241 ttgtagaacg caaacatatt gaaagagaag atattttaag ttctattatt gatttattat
     9301 gctcgtctgt aggttcttta acaaatccat ctaaaatcac taaagctatc aattccaaac
     9361 aacaacgcag cggaaaagat attgtttatc ttcccacaat taataaatat attgattttt
     9421 tagaagattc ttttcttttt aaagaggctt tgcggtttga tgtaaaagga aaatcttatt
     9481 tagatttccc aaaaaaatac tattgcgaag atataggatt gcggaatgca agaaccggct
     9541 ttcgacaaat tgaaatgccg cacattatgg aaaatatctt atacaatgaa ttaatcattc
     9601 gtggatttaa aatcgacgtg ggcgtggtct acgaaaacat taaaacggaa aaaggaaact
     9661 atcgaaaagt agctagagaa atcgacttta ttattcaaaa aggaatgaaa aaatattata
     9721 ttcaatccgc ttatacaatg gaaaatgaag aaaaagtata ttctgaatca cgttcactaa
     9781 acattactgg agatagtttt ccaaaaatta ttgtcagaag agatattcgt aaacgttttt
     9841 atgatgaaaa tggaatttta aacattggcc taattgattt tttattatca gacaatgttc
     9901 tttaatataa tgtattgcaa gttttgatat tatgttatag caaatgcagg gttgaaagcc
     9961 ctgcattttt aatattcttc ttttaaaaga aaatctatta atctacaatt gtgtggttga
    10021 taaagtgaaa atagtatatc cgttttatga atctgttata attaaaaagc caaagaaagt
    10081 taatgtctaa gcagtcattg ggaactttgt ttggcacatc ctcaccgtat gcctactaga
    10141 catgttgagg atgtttttta atgtctaatt cactctttca tcctttttac attgttttaa
    10201 agcattgatt agttttgttt catccaaagg cttagccaaa tgcatattca tacctgctat
    10261 cttgctattc attatgtctt ctacaaatgc attcgcactc atcgcaatga ttggaattgt
    10321 ctttgcatcc aatcgtttta atgcacgaat ctttcttgtc gcatctaaac catttatttt
    10381 tggcatcatg atatccataa gaataaaatc aaatttattt tcttcactct tttcaaataa
    10441 atctattact tcttgaccat cttttgcaca tacaacccga ataccattac gttctaacat
    10501 atattcaacg atttccatat tcaaatcgtt atcttctgcc acaagcacac ataaaccttc
    10561 taacgatatt tgttgatgcc tattattttt gttttcaatt ggctttccca acttaaaagg
    10621 aatctgaatc gaaaccgtag ttccaacacc cttttcactt tccaattgaa tatttccatg
    10681 catatactcc acaagctttt tggcaatagg caaaccaaga cctgtacctt gatatgtgga
    10741 tctactagaa ctatcttctt gcgcaaataa atcaaaggca tgtttaataa aatcttgact
    10801 cattccgata ccagtatctt tacatctaaa ttctaaaagc gatgtcgact catctaaaca
    10861 tttctcgttc atccatacat ggatttctcc attttccggt gtaaacttaa cggcattttg
    10921 tacaactatc aatagtattc gtgaaagata tattggatat cctctaaacc ttgaatgact
    10981 caattgacct tcataccaat ccaaaatata ttgaatattc ttttcttgtg ccaattgttg
    11041 tccatcaaca ttaatgcttt ccaataattt agtaacatca aaatcaactt cttcctctgt
    11101 aaaatgatca ctttctaaat cattcatatc caaaacatca ttgaccaaag aaatcaaata
    11161 atttaacgac cgatgaatct tatttcttgt gattttctgc agctctaaat cctttggatt
    11221 ttgatctgcc atatgaagca tacccgcaat accatttaat ggcgtacgca tatcatgaga
    11281 catactctgt aagaatcgcg tttttatttt tgcctctcgc tttgcctgtt ctgcttcaaa
    11341 gaataaattt aattctttac gctttgtctc actaattgaa cgaattgtcg caatggcttg
    11401 aatcgctttt ccattcgcat ttcttttgga aacaataata cgcaatttat gccagttgcc
    11461 atccaccatt cgatattccg ctaaaacatc ctcttctttt tcaaatcgtg aagttaatgt
    11521 ttctatattc gcaaattgat ggataatatt tcgatattcc ggtgcaacct cttgttcaca
    11581 tacgtcaaac attttttcag atgcacaccc cgttttagga attatgtcac ttcctgaaat
    11641 tctttcatat gtatctgttt caaaatcaac tttataaatc gaactataac ttttcgcaat
    11701 tgtagaaatc gtttcattac tcctctttat ttcctctaaa gcattctgca accttttctg
    11761 cgtcgctttt tcttttttga taatatcatc aatataacga aaacccaata atgcgtgaca
    11821 cgactcttca tcttcactta ccttaacaac tttcgtttca aaatattgtt gattctgcgc
    11881 atttggataa caacgataat tctgcgtaag actcacttgt gttttcaaca aagattttaa
    11941 attatcacga gaaaaccacc ataaaaactc aggctgctcc tccttaggaa tataacgcat
    12001 cgcatattga taagcatatt cataaaaatc tttataccct gaataacttc ttattaattc
    12061 actcgcatta gtttcatcct gaatatgaac aggttcaaat ttaccactgt taacttccaa
    12121 ataataaata gccgtaaagt cagaacttag actttctaga atttgttttt cttcttctaa
    12181 ccggtcattt cgttcttttt cctgttggat aatatcattc actagtttaa agcccaaaac
    12241 aaaatcatct gtctcatttg aaatctgcgc aaaacaagtc tggaaataat ctatttttcc
    12301 attaaatttt ctttgaaaaa caaccgtata tactttttct gtcttgattc gttcacgaac
    12361 gacacgataa ttcacatttc ttaaaaaatc ttctcgatct tcttcacaaa catattctga
    12421 agcatatttc tccatgctct tttgataatt attttctttg gcaaactttt caattgcttc
    12481 ttgagcaata tgatcccctt tttttgtttg ttgatacaat tcaaccgtta aatcatgatt
    12541 aattacccat accgtgacat attcatgact caaaccagaa ataaaacgct gttgaagaag
    12601 gtttttttcc ttatgcttat gaatatcaat aataaatcca caaacactta caatctttcc
    12661 tctttcattt aaaacaggct ttccaatggc acgataccaa cgatattgtt tgtctttatt
    12721 ctgaaaacga tactctacct catatgttac ggttgaatcc ctcgtatcat gaatgttttt
    12781 aaactcttct ttaacaacat caatatcttg aggatgcata tgattccaaa gcatagaaaa
    12841 cgaaatatgc gtctctcctt taaatccata aagcatctca gatagactct cactacctat
    12901 aaactctact ggcctttcat gatcatcaaa caaaacacga taacgagcct cacctaattc
    12961 tccatatagc gcatcttcac tatcttcgtt tgtatatacc catatagaag tattctcatc
    13021 attatctgaa taccgatcaa ttttcagacg aatcaaggta ccattctttt tacgatacgt
    13081 aacattcacc tgattaccag aagctaaaat attatataca taatcatagt ccaagactcg
    13141 atcaatcact tcttgataat cacaagcaac ataatgatca cgatacaatt gcatcgcatc
    13201 tgaataggaa ccttttgtgt ctttggcaaa agctcgaaat acttctggag ctaaaatatc
    13261 acgaaaagta tctgtacttc gatctaaaat ataaaccgct gcatatttag gtgctattaa
    13321 tcgcaaaaag ttttcggcat ctcttttctc ttgaagaagt tgattcatag tatgacctcc
    13381 tcatattaac attttcatta taaatgacta tttaattttt tcctattttt aaaataaaaa
    13441 ttctaacaaa aaaggaagct tcataaactg gttcaaaagt caaggacaaa tatctgacag
    13501 ttcttgttaa atgagtgtca caaatagtga tgctcatttt tttatgcttg
//
LOCUS       DBMDWL010000041        13333 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1576_length_13333_cov_2.530381, whole genome shotgun sequence.
ACCESSION   DBMDWL010000041 DBMDWL010000000
VERSION     DBMDWL010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13333)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1576_length_13333_cov_2.530381"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="ACZPDN_04320"
     CDS             <1..609
                     /locus_tag="ACZPDN_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297878.1"
                     /translation="SNKHKICVICEGNEEYEYFKRLIFLGVWNNKYAISLVNAGGNGN
                     IPARYQDRYQNGSYELVLVFCDTEKKPYEQYEDIKRQINEFHGVDNASDEVVIFGNPC
                     TMQIISKHWTSKILKTPAKAVNAGLIKEYTKVDNYKGRADQINRIMEYITKENYLEMR
                     KRVNELLLHDSIVGSTNFKRFIDFLEGEDVRWIENINNKLEM"
     gene            814..966
                     /locus_tag="ACZPDN_04325"
     CDS             814..966
                     /locus_tag="ACZPDN_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297879.1"
                     /translation="MIDDYESEIVSYGVLKRETQIWDKIKDITPTFVDAIEKAITYKT
                     FIGLDF"
     gene            complement(1050..1313)
                     /gene="rpsT"
                     /locus_tag="ACZPDN_04330"
     CDS             complement(1050..1313)
                     /gene="rpsT"
                     /locus_tag="ACZPDN_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1297880.1"
                     /translation="MPQIKSQKKRVKTNNKAHKLVVSKKSALKSSIKAVLAAVDAKDK
                     EAAVAAYNDCSSKLDKAVAKGIHHKNYASRQKARLAKAINEIA"
     gene            1426..2355
                     /gene="fmt"
                     /locus_tag="ACZPDN_04335"
     CDS             1426..2355
                     /gene="fmt"
                     /locus_tag="ACZPDN_04335"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1297881.1"
                     /translation="MENRRIIFMGTPKIASVVLKTMLENDIHVGLVVTQPDKKKGRKQ
                     QLVYSEVKEVALEYDIPVFQPVKIRNDYQEILDFAPDLIVTCAYGQIVPKEVLDLPRY
                     GCVNLHGSLLPKYRGGAPIQRAIWNGDTVSGMSLMKMAPKMDAGPVLAQKEIEILPTD
                     NSTTLFDKMGVCASELLLSHFEEICSGHAVYVEQDESRVVFSPVLSKEEEHIDLNQDD
                     EHILNQIRAFANAPGAYVLLKGKKFKILKASYVNEHNEFGLLVKIGKNKLGIGLHLGT
                     LVIETCQMEGKPVMDCASFFNGQGRNLVGNIVE"
     gene            2366..4168
                     /gene="lepA"
                     /locus_tag="ACZPDN_04340"
     CDS             2366..4168
                     /gene="lepA"
                     /locus_tag="ACZPDN_04340"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1297882.1"
                     /translation="MDQKNIRNFCIIAHIDHGKSTLADRILELTDTVEKREMKDQILD
                     SMDLERERGITIKLNAVQLVYHAKDGQDYLFHLIDTPGHVDFTYEVSRSLAACDGAIL
                     VVDAAQGVQAQTLANVYLAIENDLEILPVLNKVDLPSAQPEVVKKEIEDLIGLDCSEA
                     VEISAKSGLNVDKVLEEIVHKLPSPKGDKDAPTQALVFDSFYDSYRGIIAFVSVKNGT
                     IKPKDKIRFMATGAEYEVTEVGVRTPKEVVEDKLNCGDVGWVSAAIKNIEDVRVGDTI
                     TVASNPASEPLSGYREMHPMVYCGLYPVDNARYDDLKEALSKLKLNDAALHFEPETSQ
                     ALGFGFRCGFLGLLHMDVVEERLEREYNLSLIATSPSVIYHVYKTDGIMEEIDNPSAL
                     PEPQKIDHIEEPYVKCDIMVPKEYVGPMMDLCQKKRGEYVDLQVLDDVRMMMVYQMPL
                     SEIIYDFFDKMKSCTKGYASFDYEFSGYRTSSLVKMDILLNGQIVDALSTIVFRDFAY
                     NRGNAMAVKLKDLIPKQQFEIPVQAAIGGKIIARTNIKALRKNVLAKCYGGDISRKKK
                     LLEKQKEGKKRMKMVGSVEIPQEAFMAVLSMDDD"
     gene            4168..5247
                     /gene="hemW"
                     /locus_tag="ACZPDN_04345"
     CDS             4168..5247
                     /gene="hemW"
                     /locus_tag="ACZPDN_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1297883.1"
                     /translation="MVPSAYVHIPFCDSICAYCDFVRIQKNESFFSSWKEKLIQEIQE
                     YKISSLHTLYFGGGTPSLLSVDDFASIRNCFPTDIEEFTVECNPESLDEDKLNAYVAL
                     GVNRISLGVQSFKDDLLKICHRTHTASQAISVIENIKKSSISTFSIDLIFGLPNQTME
                     DVEKDISTFLSLDIPHLSIYSLQIEENSIFGKTGVEPCDSDLEADMFEYITKTLEAAG
                     YIHYEISSFCKPGHYSKHNLAYWQDKDFYGFGCGASGRVDGIRYDNTTSLKEYCLKGS
                     CSTYISETLAERGMDAIMMALRTKFGLNIKEWNVKYQSDFKEHYAQVLDKYREYFCFE
                     GECIYLKDAGLEILNTILVDFMLIE"
     gene            5337..5603
                     /gene="rpsO"
                     /locus_tag="ACZPDN_04350"
     CDS             5337..5603
                     /gene="rpsO"
                     /locus_tag="ACZPDN_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1297884.1"
                     /translation="MLLKSEKQAIMQEYAVHEGDTGSPEVQIAVLTEEINRLTEHFKE
                     HKHDYHSQRGLMKKVGRRKKLLAYLKGKDLDRYKALIAKLGLRK"
     gene            5671..7812
                     /gene="pnp"
                     /locus_tag="ACZPDN_04355"
     CDS             5671..7812
                     /gene="pnp"
                     /locus_tag="ACZPDN_04355"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1297885.1"
                     /translation="MAKQEFHLDFCGRGITVETGEIAKQADGAVIIRYGDTVTLSTAC
                     ASNQAKEGIDFFPLTVSFEEKLYSVGKIPGGFLRREGRPSEHATLTARMIDRPIRPLF
                     AEGFRNEVQVVNTVLSVDQDASPEMAAMFGASLALCISDIPFNGPIAGCKVGRINGEL
                     VANPTVAQMEESDIDLTVAGTAQALNMVEAGAKEVSEEDMLAALMFGHEQIKKLCAFQ
                     EEIVAACGKEKREIELYAVDETIDREVRSNFEAQIRAAVSIKEKLERYGKIDDLTDEA
                     AEMIANKECASEKDQNNAVKQAREICRGIEADEVRRLIIEDKVRPDGRQIDEIRPLNS
                     QVDLLPRVHGSALFTRGETQVLSTTTLGALNDNQIIDDLTVVDSKRFMHHYNFPPYCV
                     GETGRMGNPGRREIGHGALGERALAQVLPSVDEFPYTIRTVADVMESNGSSSQASICA
                     GTMSLMAAGVPIKAPVAGIAMGLIMDDDSGKYTVLTDIQGMEDHFGDMDFKVAGTKNG
                     ITALQMDIKVTGITKNIFEEALAQAHKARLEILDNMLACISEPRTQLSPYAPKIAMMN
                     IDPDKIKDVIGPGGKMINEIIAQCDNVKIDIDDDGKVVIYHNDYDTIEKAKQMISDIV
                     RVAKVGDVYAAKVVRLEKFGAFVNLFGNTDGLLHISKISHHRVEKVEDVLKLGDIIDV
                     KVTEIDNKGRINVSAKALLPKPKTEEEKTEA"
     gene            7887..9275
                     /gene="pepV"
                     /locus_tag="ACZPDN_04360"
     CDS             7887..9275
                     /gene="pepV"
                     /locus_tag="ACZPDN_04360"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HIR1297886.1"
                     /translation="MDFNTEVLKYKDDFLKDLNTLVSYESVRDESTKAENAPFGKNCR
                     DVLDAMLDMAKRDGFEAKDVDGYAGVVEYGKGEETFGVLGHLDIVPLGEGWTKDPLKV
                     TLENGYIFGRGVMDDKGPALAGYYALKMIRDLNIPLKKRVMLITGCDEESGMECMNYY
                     VDHAEVPQMGFVPDANFPVIYGEKGGLHVGLVSRDETVIKKLHAGSRPNIVIGKADVT
                     VDVMSYQQEDLFKFYCATQGVKGSIKRSEEGVTLHIDGAFAHAAMPYNGVNAAVLLLN
                     FIGEAFDDQLSKDLYFLLKDWMGKPVGIEKDGLYMSFLTMNTGIVNMENNETDILIDI
                     RYPNDTNADEIMAKFDAACASLTSNIKAENRGDSKPLFVDPDSRLVKDLMSVYQKYTG
                     DTFSCPITIGGGTYARKFENFVSYGPELPNEVIETDAFVGGCHQRDEGIKLDNLLQAI
                     CIYADAIVTLCS"
     gene            9283..9918
                     /gene="trmB"
                     /locus_tag="ACZPDN_04365"
     CDS             9283..9918
                     /gene="trmB"
                     /locus_tag="ACZPDN_04365"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR1297887.1"
                     /translation="MRKVKWATDYLPTANCLVKEPSKQAGNWKKLMGTDTLHIEIGCG
                     KGNYSLDMAKMYKDTGFIAIEKNESAAGIAAKKYDEDTEDKHLKLIQDDASSIGEWFG
                     PREVDVIHLNFSDPWPKKRYAKRRLSSASFLDQYKRILSRNGEIQMKTDNSALFEYSL
                     IEFQNAGFKMVEVSVDYRREKHDEDAITEYEQKFISLGQPIYRCVWRYMHD"
     gene            9911..10249
                     /locus_tag="ACZPDN_04370"
     CDS             9911..10249
                     /locus_tag="ACZPDN_04370"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR1297888.1"
                     /translation="MTDIKNKDEFLKALNNTGIQVMMFTAFWCPDCMYIKPFLPEIEE
                     EFSNLQFYSLNRDHCMDIALEYEVMGIPSFICFKDGKEISRFVSTLRKTKEEIEAFLT
                     QTIESEDKRC"
     gene            10243..10911
                     /locus_tag="ACZPDN_04375"
     CDS             10243..10911
                     /locus_tag="ACZPDN_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297889.1"
                     /translation="MLKDLMHAIFNEDIEEDDEEQEVVEEPTKEEEITPVPEVVEQPV
                     VQEEVKPQPVFTQAASSVEDNPENFMDSPIFEKAIIQPKPKKSTIFKGLDVDSIAQEE
                     TKPKQKVYKYDRHKSVKVRRVAEDLDYQPVISPIFGNVEDDKKEFDKVHDAISLQKPE
                     DEDFSQILSPMYGTYLPSMQPAKSIPEYKVEDKKANMNLSEMLEKPKKETNKQESLFD
                     KEGE"
     gene            10913..12220
                     /locus_tag="ACZPDN_04380"
     CDS             10913..12220
                     /locus_tag="ACZPDN_04380"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1297890.1"
                     /translation="MAYHFIGIKGTGMAALACILHDEHKEVTGSDIDKYIFTEDGLKA
                     RNIPIYSFSPENIKDNDTVIIGLSFDQTHPEVKAAIDNPTVKTYYYNEYLGILLESYS
                     SICVAGTHGKSTTTGILGQVLSLQDECAYLIGDGHGYMPEHANHFVLESCEFQRHFLA
                     YHPDYAIITNIELDHIDYYKDMDDYVSAFQSFANQVKKHIVVFGDNEHLKNLHYNVPV
                     TTYGMKDGNDYQAVNIEQGPFGMHFDCKHNGEILAHVELNEVGDPFMQDALGCFALAH
                     VLGMPADLIVQGLKEYKGIARRFVIDEVKDSVLVDDYAHHPTAIRLMIDAVRKKYPEK
                     KIVALYKPDRYSRLQYFLDDFAKSLNTADQVCLLDFPKNAVREDETITVTIQDLMDRC
                     PNAILLDVDDASAEKLKELAPAAFVFMSSKDIYLLRDKLEKIL"
     gene            12337..12687
                     /locus_tag="ACZPDN_04385"
     CDS             12337..12687
                     /locus_tag="ACZPDN_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297891.1"
                     /translation="MSLDIFMSTLARAFSYVLPIVGVIALIYLILFLKQLIETLKQVS
                     KTLDTANDQLKKLDAPLKTAENISTTVDEVNQSAKDAFVSVMSSLSENVQNAKDWFQN
                     KKNAKESIETKEDE"
     gene            12689..13222
                     /locus_tag="ACZPDN_04390"
     CDS             12689..13222
                     /locus_tag="ACZPDN_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297892.1"
                     /translation="MDEKQLIDDLEKESMLELEVMHESENTDNERIIEDAKSKIEKVF
                     DDLRDLLKDASDPEKVKEALSQAKKDAKDILSSTKDKVVEVSESEQFKQTVSAGKEFL
                     QGTAGLVVDGFKYGKETLRKNDSLRKVIDRADEKVEEIRTNDSVIRAVDKAEEVTGKA
                     TEALFSGLKKIFNKGDE"
     gene            13225..>13333
                     /locus_tag="ACZPDN_04395"
     CDS             13225..>13333
                     /locus_tag="ACZPDN_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1297893.1"
                     /translation="MKRITIYDVAKEADVSLATVSRVINDSNVVREDTRM"
BASE COUNT         4613 a         1826 c         2833 g         4061 t
ORIGIN      
        1 tcaaacaaac ataagatatg tgtgatttgt gaagggaatg aagagtatga atactttaaa
       61 cgcctaattt tcttgggcgt ttggaataat aaatatgcaa tttcactggt gaatgctggt
      121 ggtaatggta atattccagc cagatatcaa gatagatatc aaaatggatc ttatgagtta
      181 gtgttagtgt tttgtgatac agagaaaaaa ccgtatgaac aatatgaaga cattaagcgt
      241 caaatcaatg aatttcatgg tgtggataat gcatctgatg aagtcgttat atttgggaat
      301 ccttgtacta tgcagattat ttctaaacat tggacgagca aaattttaaa gacaccggcc
      361 aaggctgtaa atgcaggttt gattaaagag tatactaaag tagataatta caagggtaga
      421 gcggatcaaa ttaatcgtat tatggaatat atcacgaaag aaaattattt agagatgaga
      481 aaaagagtaa atgaattact cttacatgat tcgattgtcg gaagtactaa ttttaagcgt
      541 tttattgatt ttcttgaagg tgaagatgtt agatggattg agaatatcaa taataaatta
      601 gaaatgtaaa tgtaatgttt ttaaattaag ttaggtggta taaaaatgtt tcaaaaatat
      661 gtttcagttc caaagaattc aaaaggtatt tactaatttg atacgggtgt tcaggggagt
      721 gaaaatctct tattttataa tttaccgaag gtagaatttg attatttggt agataataaa
      781 atatttgaat atctgaatga aaaaggaaat ttaatgatcg atgattatga aagtgaaatt
      841 gtttcatatg gcgtattaaa acgagaaacg caaatatggg ataaaataaa agatataaca
      901 ccaacatttg ttgatgcgat tgaaaaagcc ataacttata aaacgtttat tggtttagat
      961 ttttaatgag ggtcactcta gattgagtgg ccttttatga tgaatcaaag aataaaaaaa
     1021 aagaagtcat atgacttctt aattcttcat tacgcgattt cgttaatagc ttttgctaaa
     1081 cgtgcttttt gacggctagc gtagtttttg tggtgaatac ctttagctac ggctttgtct
     1141 aatttagaac tgcagtcgtt gtaagctgca acagcagctt ctttatcttt agcatcaaca
     1201 gctgctaata cagctttaat acttgatttc aatgctgatt ttttagatac gactaatttg
     1261 tgagccttgt tatttgtttt aacacgtttc ttttgagatt taatctgtgg catgaagtgc
     1321 acctcctgac ataaatttgc aaatggatta tagcaaattt ctagatttaa tgcaacacaa
     1381 cttcaaaaaa atacgctata atactctagt attggagtga tatatatgga aaatagacgc
     1441 attattttta tgggaacacc caaaattgcg agtgtcgtac taaaaacgat gttagaaaat
     1501 gatatacatg taggcttagt ggtaacgcag ccggataaga aaaagggtag aaaacaacag
     1561 cttgtttatt cggaagtcaa agaagtggct ttagaatatg atattcctgt atttcagcct
     1621 gttaagattc gaaatgatta tcaagaaata cttgattttg cgcccgattt aattgtgact
     1681 tgcgcatatg gacaaatcgt gcctaaggaa gtattggact tacctcggta tggttgtgtc
     1741 aatcttcatg gttctttgct tcctaaatat agagggggag cacctattca aagagccatt
     1801 tggaatggag atacagtgag tggtatgtca ttaatgaaaa tggcaccaaa aatggatgca
     1861 ggtccagtac tagctcaaaa agaaattgaa atcttaccaa cagataattc aacaacctta
     1921 tttgataaga tgggcgtttg tgctagtgaa ttattacttt ctcactttga agaaatctgt
     1981 tcaggccatg cggtatatgt agaacaagat gaaagtcgag ttgtcttctc accggtttta
     2041 tctaaggaag aagagcatat tgatttgaat caagatgatg aacacatttt aaatcagatt
     2101 cgtgcctttg ccaatgcacc aggtgcttat gtgttattaa aaggaaagaa atttaaaatc
     2161 ttgaaggcaa gttatgtaaa tgaacacaat gagtttggac ttcttgttaa gattggaaag
     2221 aacaaacttg gtattggcct tcatttaggt acacttgtga tcgaaacttg tcaaatggaa
     2281 ggaaaaccag tcatggattg tgcaagcttc tttaatggac aaggaagaaa tctagtagga
     2341 aatattgtgg agtaggggga agtctatgga tcaaaagaat attcgaaatt tttgtattat
     2401 tgcacatatt gaccacggaa aaagtacatt agccgatcgt atcttagaat taacggatac
     2461 ggttgaaaag cgtgaaatga aagatcagat tctagattca atggatctag aaagagagcg
     2521 tggaatcaca attaaattaa atgccgtaca gcttgtatat catgccaaag atggacaaga
     2581 ttacttgttc catcttattg atacaccagg ccatgtcgac tttacatatg aagtgtctag
     2641 atctcttgct gcatgtgatg gggctatttt agttgtcgat gcagctcagg gtgttcaggc
     2701 acaaactttg gccaatgtat atttggctat cgaaaatgat ttggaaatct tacccgtatt
     2761 aaataaggtg gatcttccaa gtgcacaacc tgaagttgta aaaaaagaaa ttgaagactt
     2821 gattggattg gattgtagtg aagccgttga aattagtgcg aaatctggat tgaatgtcga
     2881 taaagtgctc gaagaaattg tacataaact accaagtcca aaaggggata aagatgcacc
     2941 tacacaagct ttagtctttg actctttcta tgattcctat cgtggaatca ttgcgtttgt
     3001 ttctgttaaa aatggaacca ttaaaccaaa agataagatt cgctttatgg caacaggcgc
     3061 tgaatatgaa gtgacggaag ttggtgttcg tacaccaaaa gaagtggtag aagataaatt
     3121 gaattgtgga gatgtaggat gggttagtgc agccattaaa aatattgaag atgttcgtgt
     3181 cggtgatacg atcacagtgg catctaatcc tgcaagtgaa ccactatcgg gatatcgtga
     3241 aatgcatcca atggtatatt gtggtttata tccagtggat aatgcgcgtt atgatgattt
     3301 aaaggaagct ttatcaaaat taaaattaaa tgatgcagct ttacattttg aacccgaaac
     3361 ttcacaagcc ttagggtttg gttttcgttg tggattttta ggcttattac acatggacgt
     3421 tgttgaagag cgtttagagc gtgaatataa tctaagtctt atcgcaacaa gtccttccgt
     3481 aatttatcat gtctataaaa cagatggaat tatggaagaa atcgataacc catcagcttt
     3541 accagaacct caaaaaatcg atcacattga agaaccttat gtgaagtgtg acattatggt
     3601 acccaaagaa tatgtaggtc cgatgatgga tctttgtcag aaaaaacgtg gcgaatatgt
     3661 ggatcttcaa gttttagatg atgttcgtat gatgatggtc taccaaatgc cattaagtga
     3721 aatcatctat gatttctttg ataagatgaa atcttgtaca aaaggctatg ctagttttga
     3781 ctatgaattt agtggatatc gcacaagttc attggtgaag atggatattt tattaaatgg
     3841 acaaattgtc gatgccctat ctacaattgt ctttagagat tttgcgtata accgcggtaa
     3901 tgcgatggcc gtaaaattaa aagacttgat tcctaagcag caatttgaaa ttcctgtgca
     3961 ggctgccatt ggtggaaaga tcattgcacg tacaaacatt aaggcattgc gtaaaaacgt
     4021 attggcgaaa tgttatggtg gagatatttc gcgtaaaaag aaattattgg agaaacaaaa
     4081 agaaggaaag aaacgtatga agatggtagg atcggttgag attccgcaag aagccttcat
     4141 ggccgtttta agtatggatg acgactaatg gtaccatcag cctatgtgca cattcctttc
     4201 tgtgattcca tatgtgcgta ttgtgatttt gtccggattc aaaaaaatga atcgttcttt
     4261 tctagttgga aagaaaagtt aattcaagaa attcaagagt ataagatttc aagtcttcat
     4321 actctttatt ttggtggggg aacacctagc ttattaagtg tggatgattt tgcatctata
     4381 agaaattgtt ttcctacaga tattgaggaa tttaccgtag aatgcaatcc tgaatcttta
     4441 gatgaagata aattaaatgc ctatgtagct cttggtgtga accgaattag tttaggtgta
     4501 caaagcttta aggatgatct attaaagatt tgtcatagaa cacacacagc aagtcaggca
     4561 atttcggtta ttgaaaatat caagaaaagt tcgatatcga ctttttccat tgatttgatt
     4621 tttgggcttc caaatcaaac aatggaggat gtggaaaaag atatttccac atttttaagc
     4681 ttagatatac cacacttatc tatctattct ttgcagattg aagaaaattc tatctttgga
     4741 aagacagggg tagaaccttg tgatagtgat ctagaagccg atatgtttga atatatcacg
     4801 aaaacacttg aagcagccgg atatattcac tatgagattt ctagcttttg taagccaggt
     4861 cattattcca agcacaatct agcatattgg caagacaaag acttttatgg ctttggctgt
     4921 ggtgcaagtg gaagagttga tgggattcgt tatgataata caacaagttt aaaggagtat
     4981 tgtttgaagg gatcttgttc tacttatatt tctgaaacac ttgctgaacg aggtatggat
     5041 gcgatcatga tggctttacg tacaaagttt ggattgaata taaaagaatg gaatgtaaag
     5101 tatcaaagtg attttaaaga acattatgcg caagtattag ataagtatcg tgagtatttt
     5161 tgttttgaag gggaatgtat ctatttaaag gatgctggtc ttgaaatatt aaatacgatt
     5221 ctagttgatt ttatgttgat tgaatgatat aatttaatag cttttgtact gagagtctgg
     5281 aaacttccaa cctttcgctc ggtagaaaag gactaatata catggaggaa gaaaaaatgt
     5341 tattaaaatc tgaaaaacaa gcaattatgc aagagtatgc agtacacgaa ggtgacacag
     5401 gttctcctga agttcaaatc gctgtattga cagaagaaat caaccgttta actgaacact
     5461 tcaaagaaca caaacacgat taccactcac aacgtggatt gatgaaaaaa gttggtcgtc
     5521 gtaaaaaatt gttagcttac ttgaagggta aagatttaga tcgttacaaa gcattgatcg
     5581 ctaaattagg tttaagaaag taatcgaaaa gattactttt tttttaagca tttatttgat
     5641 agaatataaa agtatgattg aggtgaaaaa atggcgaaac aagagtttca tttagatttt
     5701 tgtggtcggg ggatcacagt agaaactggg gaaatcgcaa aacaggcaga tggtgctgtt
     5761 attattcgtt atggagatac agttacatta tcaactgcat gtgcatcgaa ccaggcaaaa
     5821 gaagggattg actttttccc tttaacagta agttttgaag aaaagttata ttctgttgga
     5881 aagattccag gaggattttt acgtcgtgaa ggaagaccaa gcgaacatgc gacattaaca
     5941 gctcgtatga tcgaccgtcc aatccgtcct ttatttgcgg aaggattccg taatgaagta
     6001 caagttgtca acacagtatt aagtgtggat caagatgcaa gtccagaaat ggctgccatg
     6061 tttggtgctt ctttggcatt atgtatttct gatattccat tcaatggacc tattgctggt
     6121 tgtaaagttg gacgtatcaa tggagaattg gttgccaacc ctacagttgc tcaaatggaa
     6181 gaaagtgaca ttgatttaac agttgcaggt actgcacaag ctttaaacat ggttgaagca
     6241 ggtgctaaag aagtgagcga agaagatatg cttgcggcat tgatgtttgg acatgaacaa
     6301 atcaaaaaat tatgtgcatt ccaagaagaa attgttgcgg cttgtggtaa agaaaaacgt
     6361 gaaatcgaat tgtacgcagt tgatgaaaca attgatcgtg aagttcgttc taactttgaa
     6421 gcacaaattc gtgcggctgt ttcaattaaa gaaaaattgg aacgttatgg taagattgat
     6481 gatttaacag atgaagcggc tgaaatgatc gcaaacaaag aatgtgcatc tgaaaaagat
     6541 caaaacaatg cggttaaaca agctcgtgaa atttgtcgtg gtattgaggc ggatgaagta
     6601 cgtcgtttga ttattgaaga taaagttcgt ccagatggtc gtcaaattga tgaaattcgg
     6661 ccattgaact cacaagtgga tttacttcct cgtgttcatg gttcagcatt atttactcgt
     6721 ggtgaaacac aggtattatc aacaactaca ttgggtgcat taaatgataa ccaaatcatt
     6781 gatgacttaa ctgttgtaga ttcaaaacgt ttcatgcacc actataactt cccaccatat
     6841 tgcgttggtg aaacaggacg tatgggaaat ccaggacgtc gtgaaattgg tcatggtgct
     6901 ttaggtgaaa gagctttagc tcaagtatta ccaagcgtag atgaattccc atatacaatt
     6961 cgtactgtgg cagatgttat ggaaagtaat ggatctagtt ctcaggcaag tatttgtgca
     7021 ggaactatgt ctttaatggc agctggtgtt ccaatcaaag ctccagtagc tggtattgcg
     7081 atgggattga tcatggatga tgattcaggt aaatatactg tattaacaga tattcaaggt
     7141 atggaagacc actttggaga tatggacttt aaggttgctg gaactaaaaa tggtatcaca
     7201 gctttacaga tggatatcaa agtaacaggt atcactaaaa atatctttga agaagcttta
     7261 gcacaagctc ataaagctcg tctagaaatc ttagacaata tgctagcatg tatttctgag
     7321 ccaagaacac aattgagtcc atatgcacct aagattgcga tgatgaacat tgatccagat
     7381 aagatcaaag atgtgatcgg acctggtgga aaaatgatca atgaaatcat tgcacaatgt
     7441 gacaatgtaa aaattgatat tgatgatgat ggaaaagttg tgatttatca taatgattat
     7501 gatacaatcg aaaaagcaaa acaaatgatc agtgacattg ttcgtgtagc taaagttggc
     7561 gatgtatatg ctgctaaagt tgttcgttta gaaaaatttg gtgcttttgt aaacttattt
     7621 ggtaacacag acggtttact acacatctct aaaatttcac atcaccgtgt agaaaaagta
     7681 gaagatgtat taaaacttgg tgatattatc gatgttaagg ttacagaaat cgataataaa
     7741 ggccgtatca atgtaagcgc aaaagcttta cttccaaagc caaaaacaga agaagaaaaa
     7801 acggaagcgt aaaagtaagg aactcgttaa aaacgagttc ttttttcttg actatgtggt
     7861 aaaatagttg ggtagaggtg aaaattatgg attttaatac agaagtactt aaatataaag
     7921 atgatttctt aaaagactta aatacacttg tatcgtatga aagtgttcgt gatgaatcga
     7981 caaaagctga aaatgcacct tttggaaaaa attgtcgtga cgtattagat gcaatgttag
     8041 atatggctaa acgtgatggt tttgaagcca aagacgttga tggatatgca ggtgttgtgg
     8101 aatatggaaa gggtgaagaa acatttggtg ttttagggca cttggacatt gtgccactag
     8161 gagaaggatg gacgaaagat cctttaaaag taacattgga aaatggatat atctttggac
     8221 gtggtgtcat ggatgataaa ggtcctgcac tagctggtta ttatgcatta aaaatgattc
     8281 gtgatctaaa catccctttg aaaaaaagag taatgttgat tacaggatgc gacgaagaat
     8341 caggtatgga atgtatgaac tactatgtag atcatgccga agttcctcaa atgggatttg
     8401 taccagatgc caacttccca gtaatttatg gtgaaaaagg tggcttacat gtaggattag
     8461 taagtcgtga tgaaacggta attaaaaaac ttcatgcggg aagccgtcca aacattgtca
     8521 ttggtaaagc ggatgtgact gtggatgtga tgagttatca acaagaagat ttatttaaat
     8581 tctattgtgc tacgcaaggt gttaagggtt caatcaaaag aagtgaagaa ggagttacac
     8641 tacatattga tggtgccttt gcacatgcag ctatgccata taatggtgtg aatgccgctg
     8701 ttctattatt gaactttatt ggtgaagcct ttgatgatca actatcaaaa gatttatatt
     8761 tcttattaaa agactggatg ggaaaacctg ttggcattga aaaagatggt ttatatatgt
     8821 ctttcttgac aatgaataca ggtattgtga atatggaaaa taacgaaaca gatatcttaa
     8881 ttgatattcg ttatccaaat gatacaaatg cagatgagat catggctaaa tttgatgcag
     8941 cttgtgcaag cttaacttca aatatcaaag ccgaaaatcg tggagattca aaaccattgt
     9001 ttgtggatcc agattctcgt ttagtaaaag atttaatgag tgtctatcaa aaatatacag
     9061 gggatacatt ctcttgtcct atcacaattg gtggtggaac atatgcacgt aaatttgaaa
     9121 actttgtatc atatggtcca gaacttccaa atgaagtgat tgaaacagat gcattcgtag
     9181 gaggatgtca ccaacgtgat gaaggtatta aattagataa cttgttgcag gctatctgta
     9241 tttatgccga tgcgatcgtg acattgtgta gctaatatga gaatgcgtaa agtaaagtgg
     9301 gcaacggatt atttgcctac cgcaaattgt ttagtaaaag aaccatctaa acaggcagga
     9361 aattggaaaa agttgatggg tacagatact ttgcatattg agattggttg tggaaaagga
     9421 aattattctt tagacatggc taaaatgtat aaggatactg gattcattgc gattgaaaag
     9481 aatgaaagtg ctgcgggtat tgccgctaaa aaatatgatg aggatacaga ggataaacat
     9541 ttgaaattga tccaagatga tgcgagtagc ataggagaat ggtttggacc tcgtgaagtc
     9601 gatgtgatcc acttgaattt ctcagatcca tggcctaaaa agcgttatgc gaaaagacgt
     9661 ttatccagtg cttccttttt agatcaatat aagcgcatct tatcaagaaa tggcgaaatt
     9721 caaatgaaaa cggacaacag tgctttgttt gaatatagct tgattgaatt tcagaatgcg
     9781 ggatttaaga tggttgaagt cagtgtggat tatcgtcgtg aaaaacatga tgaagatgcg
     9841 attacagaat atgaacaaaa attcatttct ttaggacaac caatctatcg ttgtgtttgg
     9901 agatatatgc atgactgata ttaaaaataa agacgaattt ttaaaagcac taaacaatac
     9961 aggcattcaa gtgatgatgt ttacagcgtt ttggtgtcca gactgtatgt acatcaaacc
    10021 tttcttgcct gaaattgaag aagaatttag caatttacaa ttctattcct taaatcgtga
    10081 tcattgtatg gatattgcgc ttgaatatga agtgatgggt atccctagct ttatatgttt
    10141 taaggacgga aaagaaattt caagatttgt atcgactctt cgaaagacaa aagaagaaat
    10201 agaggcattt cttactcaga ctatagaaag tgaggataaa agatgttaaa ggacttaatg
    10261 catgcgattt tcaatgaaga tattgaagaa gatgatgaag aacaagaagt tgtagaagaa
    10321 cctacaaaag aagaagaaat tacacctgta cctgaagttg tagaacaacc tgttgtacaa
    10381 gaagaggtaa aaccacagcc tgtatttaca caagctgcaa gttcagttga agataatcct
    10441 gaaaacttta tggattctcc aatttttgaa aaggcgatta ttcaacctaa accaaagaaa
    10501 tctacgattt ttaaaggctt agatgtggat tcaattgccc aagaagaaac aaaaccgaaa
    10561 caaaaggtat ataaatatga tcgtcataaa tcagtaaagg tacgtcgtgt ggcggaagat
    10621 ttagattatc aacctgttat ttctccaatt tttggaaatg ttgaggatga taaaaaagaa
    10681 tttgataaag tacatgatgc gatttcttta caaaaaccag aagatgaaga tttctcacag
    10741 attttatcgc caatgtatgg aacatacctt cctagtatgc aacctgcaaa atcgattcct
    10801 gaatataagg tggaagataa aaaggcgaat atgaatcttt ctgaaatgtt agaaaagcca
    10861 aaaaaggaaa caaataaaca agaatcatta ttcgataaag aaggtgaata gaatggctta
    10921 tcattttatt ggtattaaag gaacaggtat ggctgcctta gcatgtatct tacatgatga
    10981 acataaagaa gtaacgggta gtgatatcga taaatatatt tttacagaag atggattaaa
    11041 agctcgtaat attcctattt attcatttag tcctgaaaat attaaggaca atgacactgt
    11101 aattattggt ttatcttttg atcaaacaca tcctgaagtg aaagcggcca tagataatcc
    11161 aactgtaaaa acatattatt ataatgaata tttaggtatt ttattagagt catatagctc
    11221 aatttgtgtg gccggtacac atggtaaatc aactacgact ggtattttag gccaagttct
    11281 ttctttacaa gatgaatgtg cctatttaat tggggatggc catggctata tgccagaaca
    11341 tgccaatcat tttgtgctag agtcttgtga atttcaaaga catttcttag cctatcatcc
    11401 agattatgcg attattacaa atattgaatt agatcacatc gactattata aagatatgga
    11461 tgattatgta tcggcattcc aaagttttgc caaccaagtt aaaaaacaca ttgtggtatt
    11521 tggggataac gaacacttga agaatttgca ttacaatgta cctgtaacaa cgtatggtat
    11581 gaaggatgga aatgattatc aagccgttaa tattgaacaa ggtccatttg gcatgcattt
    11641 tgattgtaag cacaatggtg aaattttagc tcatgtagaa ttaaatgaag taggagatcc
    11701 atttatgcag gatgccttag gatgttttgc gctagcccat gttttaggaa tgccggctga
    11761 tttgattgta caaggcttaa aagaatataa aggaattgca cgtcgatttg tgattgatga
    11821 agtcaaggat agtgtattag tggatgacta tgcacaccat ccaaccgcta ttcgtttaat
    11881 gattgatgcc gttcgtaaaa aatatccgga aaagaaaatt gttgcgttgt ataaaccaga
    11941 tcgttattca cgtctacaat atttcttgga tgactttgcg aaaagcttaa atacggcaga
    12001 tcaagtatgt ttacttgatt ttcctaaaaa tgccgtaaga gaagatgaaa caatcacggt
    12061 cacaattcaa gatttaatgg atcgttgtcc aaacgcaatc ttattagatg tagatgatgc
    12121 ttcagctgaa aagttaaaag aattagctcc tgcagcattc gtctttatgt caagtaaaga
    12181 catctatcta ttaagagata aattagagaa gattttataa gagtatcggt agatactctt
    12241 tttttaatat tgtagcgttt acagggttgt gaaaaaagtt tcaaaaggaa tagattagtt
    12301 agaaagctta tgttataatc tttctgaggt ggtattgtga gtctagatat ctttatgagt
    12361 acattagctc gtgcattttc atatgtttta ccaatcgttg gtgttatcgc attaatttat
    12421 ttgattttgt ttttaaaaca gttgattgaa actttaaaac aggtgtcaaa aacattggat
    12481 acagccaatg atcaattaaa aaaattagat gcaccattaa aaacagcaga aaatatttct
    12541 accacagtgg atgaagtgaa ccagagcgca aaagacgcgt ttgtgagtgt gatgagttca
    12601 cttagcgaaa atgtacaaaa tgccaaagac tggtttcaaa ataagaaaaa tgcaaaagaa
    12661 agtattgaaa caaaggagga tgaatgacat ggacgaaaaa caattaattg atgatttaga
    12721 aaaagaatcg atgctagaac ttgaagtgat gcatgaatcc gaaaatacag ataatgaaag
    12781 aattattgag gatgcaaaga gtaaaattga aaaagtgttc gatgatttgc gtgatttatt
    12841 aaaagatgct tctgatcctg aaaaagtaaa ggaagcttta tcgcaagcta agaaggatgc
    12901 aaaagatatt ctttcttcaa caaaagataa agtagttgaa gtcagtgaga gtgaacaatt
    12961 taaacaaact gtttcggctg gtaaagaatt tttacagggc acagctggat tggtagtaga
    13021 tggctttaaa tatggaaaag aaacattaag aaaaaatgat tctttacgta aagtgattga
    13081 tagagcggat gaaaaagtcg aagaaattcg tacaaatgat tctgttattc gtgccgttga
    13141 taaagcagaa gaagtaactg gtaaagctac agaagcattg tttagtggat tgaagaaaat
    13201 atttaacaag ggggacgaat aggaatgaaa agaattacca tttatgatgt tgccaaagag
    13261 gcagatgtct cattagcaac tgtatcacgt gttattaatg atagtaatgt agtacgtgaa
    13321 gatacacgta tgc
//
LOCUS       DBMDWL010000042        13320 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1580_length_13320_cov_2.810500, whole genome shotgun sequence.
ACCESSION   DBMDWL010000042 DBMDWL010000000
VERSION     DBMDWL010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1580_length_13320_cov_2.810500"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(97..519)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_04400"
     CDS             complement(97..519)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIR1297894.1"
                     /translation="MKNENYHRASHCKYLVQYHIIWCPKFRCDGMDETLKKILSEVCD
                     AYGYIIKALEVMPDHIHIFVDCPVTTAPCDVVRTLKSMSAIQLFKAYPELKRFYARCG
                     KLWSNGYFISSVGHISEAAIKKYIEEQKNDTTRIESLS"
     gene            complement(509..697)
                     /locus_tag="ACZPDN_04405"
     CDS             complement(509..697)
                     /locus_tag="ACZPDN_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297895.1"
                     /translation="MPRPRTNNVQINIAIPGEWKKELENLARIYSVEEGKTITFLDLM
                     RRGIQEKYQLKDVNAHEE"
     gene            923..2764
                     /locus_tag="ACZPDN_04410"
     CDS             923..2764
                     /locus_tag="ACZPDN_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006061369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR1297896.1"
                     /translation="MKLYYDKRLKDPTYYIQQGFRNGKKTTTKNIKRLGKHSELLLIT
                     DNPLEYAKNEVKKMNEEYRSGRSEFIVTMDFNERIPSTDSPCSNSTSLNIGYLYLKDI
                     YAKLNLSDFFKSVSSDRKITYDCNKICQFLTYARILDPASKYGTYDKLDTYYEKPQVE
                     YQHMIRFLDILDRNSDKYLKHLFDNSENIVKRDTSVMYYDCTNYFFETEKPDEEIVDE
                     VTGEIILGLRQFGISKENKTSPIVEMGLIMDSRGIPISMCIHPGNTNEQLTAVPLEKE
                     VIKMTGSKKFIYCADAGLGSYNIRKFNDMGGRAYIVTQSVKKLGQEIKDIVFNDSNYR
                     LLSNDDAITLKEMRTFNKKDANNLSLYNDFAYKVIPANTAMDTGLYEEKVYKNGRTKK
                     VKAKGTLHQYIIVTFSRKMMEYQRTIRERQLERAKKLLRLKDPEKIKKGPNDIRRFLK
                     NTSSDTANYVLDMDKIHEEEKYDGFYAVATNLDDSAKDILAVAQNRYKIEDCFRIMKT
                     NFDARPVFLRKPERIRAHFLICYTALLIYRLMECKLDDNLTHVTTSNLIKTLRNMNVV
                     NMDDMYYKSIYSGSQALDALERCFELQLNRKYYRPSDLNKIVKKFSK"
     gene            2901..3686
                     /gene="rhuM"
                     /locus_tag="ACZPDN_04415"
     CDS             2901..3686
                     /gene="rhuM"
                     /locus_tag="ACZPDN_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014691.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HIR1297897.1"
                     /translation="MAFNSTASYGVESHLNITREIDYYNLDMIISVGYRVKSQNGVIF
                     RKWATSILHDFMIKGYAVNQKRLDVLNKTIAIQSKMLASALNIEEKEVLNVIEAYSNA
                     LSLLDDYDHGTIPKPDGIASIYQLTYEECRELIDSMKYGNFSDVFGVEKEAGKLNGII
                     AAVYQNVFKTELYPSIEEKAANLLYFLIKDHPFVDGCKRIGASIFLEFLNKNKHLIIN
                     GKQIISDSALVAITLMIAESRPEEKETMVNLVMNFLKCEFCVN"
     gene            3827..4108
                     /locus_tag="ACZPDN_04420"
     CDS             3827..4108
                     /locus_tag="ACZPDN_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016211.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIR1297898.1"
                     /translation="MKKILYIMIGCISLGLGIIGVILPILPTVPFVLLAAFCFARSSE
                     RLDGWFKNTKLYRENNIKNGMTKQAKIRIMCSVTLLMSIGFIMMGLKGL"
     gene            4179..4505
                     /locus_tag="ACZPDN_04425"
     CDS             4179..4505
                     /locus_tag="ACZPDN_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297899.1"
                     /translation="MKRVLNYSLAISFILTIMVPITGIIVHKLASTLFLVLALIHVFI
                     YRKRLDLKYILIIVLTIVSFVLGILGLVLVEYPIILTLHRTVSIVLIILLAIHIYKFH
                     RRLAIA"
     gene            4748..5389
                     /locus_tag="ACZPDN_04430"
     CDS             4748..5389
                     /locus_tag="ACZPDN_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014373.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR1297900.1"
                     /translation="MYSELEYLNWIFRIVLATLCGCAIGYERTSRNKSAGIRTHAIIA
                     LGSSMFMIISKYAFFDVSTADGARVAAQIVSGIGFLGAGVIFVKDDLIQGLTTAAGMW
                     TTSGIAACIGAGLIFIGVFATLFLVVLQKIFHYPFFNQIDHPNVLIQTNIVSKNDEIY
                     KIFEKYGIEVNEIKIDRNLDLMTFDTNSRWIDHKNELFQDLMSIDTVTKVSIL"
     gene            5404..6252
                     /locus_tag="ACZPDN_04435"
     CDS             5404..6252
                     /locus_tag="ACZPDN_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1297901.1"
                     /translation="MADYFDDVNHQMEKLLRGLKIMKIKDVIEILKQEGTWVRWNRCT
                     RDRILFGNENQEVTKIGVCWVATNKVIEQALEKGINFIISHENIFYTTGTHLETKLVE
                     SIEYKKELLSKGNICVYRCHDVWDSIPKYGVSDVWAKKLGFDFRNRVINSYYQSANIP
                     KQTVSQLATHVANVLKDDGEDGVYVFGDVNKEVSHLAIGTGAGTDIFEMLEFNPDVVI
                     VADDGITNYKDAQYAIDHDLPMIVVNHAGCEIGGLKNMVNYFNDKLPELDVEYLDEGF
                     KISYFK"
     gene            6274..6666
                     /locus_tag="ACZPDN_04440"
     CDS             6274..6666
                     /locus_tag="ACZPDN_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297902.1"
                     /translation="MSDEKLEWRWALVGNIVEKHEYGENHEIRYGARKFRGGTRVVLA
                     PPCWGDGYEQVNVIGLSRYSRKYKKSVIRRNLITNFRVKKIYSPTILKIMEETENNDS
                     HCWWWDNTDEAYQDIVRLANSLNSKEAD"
     gene            complement(6689..7207)
                     /locus_tag="ACZPDN_04445"
     CDS             complement(6689..7207)
                     /locus_tag="ACZPDN_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297903.1"
                     /translation="MSEQNKKEFQTPYEEYRVKAGYTRESASEELNGISPDKIYRIEK
                     GKQTAAPDIVLQLADLYHAPELCNYHCTHKCEIGQKYIPQVDVQDLPSIILSTVATLN
                     DIQPLVNRLIQITMDGKISDDEIPDFALISTKLDEISTASDVLRLWIEKTIQVNKLNG
                     TLFYEEVDKLKK"
     gene            7348..7479
                     /locus_tag="ACZPDN_04450"
     CDS             7348..7479
                     /locus_tag="ACZPDN_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297904.1"
                     /translation="MKTVDNRLRMIRLIEKIERNRKFADQMHVKDVSEFKKQSNANK"
     gene            7492..8436
                     /locus_tag="ACZPDN_04455"
     CDS             7492..8436
                     /locus_tag="ACZPDN_04455"
                     /inference="COORDINATES: protein motif:HMM:NF019976.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HIR1297905.1"
                     /translation="MMNRESLLSSLKGLSKNVYSREELLPEMDLLEEEMAQIVFEDGL
                     DIPSEYVHMSYIMKRLHIMNNEAGNVADQELKKLSRLASIFSNHVRAQQSGITGEDRV
                     EQYLDYMKSNHVVLRNLQLSDGVYKTELDFLVLKQGVATIVEVKNSKRDVYIDEAGDM
                     YKVGRYENFDSHLGAKMDFRAEIIQQLLKDAGYENMRIEKVVVFTNNRIQIKKDYHGF
                     RVCFLSRLPHLIDDFYGTGVIQFTKHLQQMADYIQSKDLNEYYPFEMDVQEFKEAFVD
                     AYLKIKGYDQPVEDGLFTRFKTVIHSLFNMPTTQHVSY"
     gene            8455..9087
                     /locus_tag="ACZPDN_04460"
     CDS             8455..9087
                     /locus_tag="ACZPDN_04460"
                     /inference="COORDINATES: protein motif:HMM:NF025380.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR1297906.1"
                     /translation="MNYLDDIDYEDVSFIKKHPEIKGVFITIAVLVISFLSIGIYKLA
                     NPPKEEVNTINVEIEKKGKLIVAETEAHDTDVATSQAMIGPLSLWYDNSVEYDCYAKS
                     TVTYDLDQTKATANTLTKVVTVKAPKQEVETHLMLDTYKSINENQTVFNPVDDKDLNK
                     SQRKLQKRLNDAAMKSELPQEAETSFEEHMTKWITDQDAYKDYTVKFKYA"
     gene            9289..9765
                     /locus_tag="ACZPDN_04465"
     CDS             9289..9765
                     /locus_tag="ACZPDN_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1297907.1"
                     /translation="MLKNLFKVQKKIQIYSPVNGKCVDLKDVNDQMFAEKIMGDGIAF
                     KYNENTIYSPCKGKIVAVASTKHALGIETEDGIEILIHVGLETVEFNGKGFELLVNQN
                     DKVTTGQPLLKIDRDFFETHHTDLTTPVVVTSKGLNIEFTNKEEVTIKDVVMMINR"
     gene            9772..10608
                     /locus_tag="ACZPDN_04470"
     CDS             9772..10608
                     /locus_tag="ACZPDN_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIR1297908.1"
                     /translation="MKVVKCINNNVAICLDDDNNELVAFGKGIGFKKPPFEIDVAVIQ
                     KTYYGIDENYVHMINEVPEEILLLSEEIIKYAENELDYIFSPNIIFTLADHINFAIVR
                     CKEKLNITLPIVQDIKFLYENEYAIGKYALSLIEKRLNIKLPLEEASFISLHIINAEA
                     RKAGEIDGYAENKVIISEITKLIENKFDISINKDGANYSRFVSHIQYLLKRIGSKEEI
                     KSENKELYMTMKNSYPDTSDCVDSISEYLFELFKWRLSIEEKLYLILHINRLCSREDC
                     YQ"
     gene            10824..12227
                     /locus_tag="ACZPDN_04475"
     CDS             10824..12227
                     /locus_tag="ACZPDN_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014437.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1297909.1"
                     /translation="MSNYSKNAEDIIKYIGEGKNIEEVKHCFTRLRFKVKDNSKVNVE
                     MLEKIPCVVRVMKTMGQIQVVVGNEIDEVYKAVEKYLEGTSIDVSKLEEPVETKEEKG
                     PKGIKYYGSQILNAISQTISPILMGMVVAGLFKAILTILTLTNIVSTDSQTYTLLNLM
                     GDSFFYFMPVLVAYSASKYFKCNTVLSLMLAGILLHPDFINLVNNGESISLFGLPVHA
                     ISYSSSLLPVLFTVWVQSKLEKLVSKTFFTKLGMLSLFPTFLIMAPLILVVTGPFGSI
                     VGELIANAMLVIYNKYAVLGVFAICFLMPLFIWTGSHWVFMPVALSNMTNLGFDPFLW
                     LGFTAWNFSQLAVSTAIMLKTKDKDLKQIAGSAAFSIATAGISEPCAYGLTLKYKRPI
                     IPSFIGCAVGGLFFGIFKVKVFQMINVSLLSLPQFLDPAGGNNFMLAIIRIVLVFTVT
                     FVLTWFFGFDDKKDLGK"
     gene            12236..12937
                     /locus_tag="ACZPDN_04480"
     CDS             12236..12937
                     /locus_tag="ACZPDN_04480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1297910.1"
                     /translation="MKINDRIYWISGGPWGYLGNAYAIKHSNGYVLVDTGSPEALDVI
                     VKNLKYWNIDENKITHILITHAHDDHTGCAHYFQKCGAKIVMGKEDAKMLEDGHLGEY
                     SPCTNHVMPACKADILIDKDEILQIGDLEFEILCLPGHTDGTLLYFVRIDDENVMFSG
                     DMFYTDGKWGEFTRTGWKGDLSYNSKKLGESFEKLYKLKEEPDLALAGHGIPRFGKEA
                     EVIRLAYKYYLKFDR"
     gene            12927..>13320
                     /locus_tag="ACZPDN_04485"
     CDS             12927..>13320
                     /locus_tag="ACZPDN_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR1297911.1"
                     /translation="MIDRIYNGVLDLNLKDIMIKAIIFDFNGTMIFDQEILEKSWTLF
                     LKNLIHRKPTDEEFSNYIHGRNIEESIHYFLGDDVENINTLKLEKEKIYRDFCKADKE
                     NYKLVDGLPEFLDDLKMKNYVCAIATASG"
BASE COUNT         4599 a         1694 c         2564 g         4463 t
ORIGIN      
        1 ccagctttac gaaccaaatc ggcataatga aaaatctgag acttttctac ataagtcaga
       61 ttcgtttcca atactaatac atgtctatct gaacatctac gataggcttt caattcgggt
      121 tgtatcattt ttctgctcct cgatatattt cttgattgca gcttcactga tatgccctac
      181 agaagatata aaatatccat ttgaccataa ttttccacat ctcgcataga atctcttcaa
      241 ttccggatac gccttgaaca attggattgc actcatagat ttcaatgttc gaacaacatc
      301 acaaggtgca gtcgttacag gacagtctac aaaaatatgt atatggtcag gcatgacctc
      361 taaagctttt ataatatatc catacgcatc acaaacttca gaaagaatct tctttaatgt
      421 ttcatccata ccatcacatc tgaactttgg acaccatata atatgatact gaactaaata
      481 cttacaatga gaagcacgat gataattctc attcttcatg agcattcaca tcttttaact
      541 gatacttttc ctgaatacct ctacgcatca aatccagaaa agtgatcgtt ttaccttctt
      601 caaccgaata gattctggca aggttttcca gttctttttt ccattcacca ggaatggcaa
      661 tattgatctg aacattgttc gttctaggtc taggcatgat atcaactccg agctatttat
      721 acatatatta tatactttta atacgcatta tacaatacaa aaagtggctt tcaactccac
      781 agcaagcggg agtttaacac ttgctcataa ccgaaatagc caaatgtcgt ttatgtacgg
      841 cttttttttc gttaaatttt tattttttac agttgtatgt gttgtaatta tgatataata
      901 gttgtaaagg atggctgctt ctatgaaact gtattatgat aaacgactta aagatcctac
      961 ttactatatt cagcagggat ttagaaatgg taaaaaaacg acaaccaaaa atatcaaaag
     1021 attaggtaaa cattctgagc ttcttcttat tacggataat cccttggaat acgctaaaaa
     1081 cgaagtaaaa aaaatgaatg aggaatatcg ttctggcaga tctgaattca ttgttactat
     1141 ggactttaat gaaagaatcc cttctacaga ttctccttgt tcgaattcta ctagtttgaa
     1201 cattggatat ctgtatctta aggatatcta tgccaaattg aatctgtctg atttttttaa
     1261 atcagtttct tctgaccgaa agatcaccta cgactgtaat aaaatctgtc agtttcttac
     1321 ctatgcaagg attctggatc ctgccagtaa atatggaaca tacgataaac ttgacacata
     1381 ctatgagaaa cctcaggttg aataccaaca tatgattcgt ttcttggata ttctagacag
     1441 aaacagtgac aaatatttaa aacatctttt tgacaattcc gaaaacatcg ttaaacgaga
     1501 tacttcagtt atgtactatg actgtacaaa ctatttcttt gaaacagaaa agcctgatga
     1561 ggagatcgtt gacgaagtta ctggagaaat tatccttgga cttcgtcagt tcggaatcag
     1621 taaggaaaac aagacatctc ctattgttga aatggggctt atcatggaca gccgtggaat
     1681 ccctatttct atgtgtattc atccaggaaa cacaaatgaa cagcttactg ccgttccact
     1741 tgaaaaagaa gttataaaaa tgaccggaag caaaaagttc atatattgtg cggatgcagg
     1801 acttggatcc tacaatatcc gtaaattcaa tgatatgggt ggtagagctt atatcgttac
     1861 acagtcagta aaaaaacttg gacaagagat caaagatatc gtattcaacg attcaaacta
     1921 ccgtctattg tcaaatgacg atgcgatcac attgaaagaa atgcgaacat ttaataaaaa
     1981 agacgcaaac aatctaagtc tatacaatga ttttgcctat aaagtgattc ccgccaacac
     2041 ggccatggat acaggacttt atgaagaaaa agtatacaag aacggtcgaa caaaaaaagt
     2101 aaaagccaaa ggaacattac atcaatatat cattgtcact ttctctagaa agatgatgga
     2161 ataccaaagg actatccgcg aaagacagct tgaacgtgct aaaaagttat taagacttaa
     2221 agatcctgaa aagataaaaa aaggacccaa cgatatacgt cgattcctta agaacacatc
     2281 atctgataca gctaattatg tattggatat ggataaaatc catgaagaag aaaaatacga
     2341 tggattctat gccgtcgcaa caaatcttga cgattcagcc aaagacatcc tagcagtagc
     2401 tcaaaataga tacaaaatcg aagactgttt tagaatcatg aagactaatt tcgatgcaag
     2461 accagtattc ttgagaaagc cagaaagaat acgtgcacat ttcttgatct gctacactgc
     2521 actacttata tacaggctta tggagtgcaa gttggatgac aacttaactc atgtaacaac
     2581 atcaaaccta ataaaaacat tacgaaatat gaatgtagta aatatggatg acatgtacta
     2641 caaatccatc tatagtggaa gtcaggcatt agatgcatta gaaagatgtt tcgagctaca
     2701 actaaacaga aagtactata gaccaagtga tttaaacaaa atagtaaaaa aattttcaaa
     2761 atagaaatca catacaacat ttcaaaaaaa tgaaagtgct agattaaacc ttgaataaag
     2821 gctttctagc actttttact ttttcaactg tcaaactcgg gatattatat acttttaata
     2881 cgcattatac aatacaaaaa gtggctttca actccacagc aagctatgga gttgaaagcc
     2941 acttaaatat aacaagagaa atagattatt acaacttaga tatgattatt tccgttggct
     3001 atcgagtaaa atcacaaaat ggtgtaattt tccgaaaatg ggcgacatct attcttcatg
     3061 atttcatgat caaaggttat gcagtaaacc aaaaacgttt agatgtttta aataaaacaa
     3121 tcgcgattca atccaagatg cttgcttctg ctttaaacat tgaagaaaaa gaagtgttga
     3181 atgtgattga ggcttattca aatgcattat cattactgga tgattatgat catggaacga
     3241 ttcctaaacc ggatggtata gcttcaattt atcagttaac ctatgaagaa tgtcgtgaat
     3301 tgattgattc tatgaagtat ggtaactttt cagatgtatt tggtgttgaa aaagaagcag
     3361 gtaaattaaa tggaataatt gcagcggtat atcaaaatgt ttttaaaact gaattgtatc
     3421 caagtattga agaaaaagct gcaaaccttc tttacttttt aattaaggat catccttttg
     3481 tggatggttg taaacgtata ggtgcatcta tattcttaga gtttcttaat aagaataaac
     3541 atcttattat caatggaaaa cagattattt cagatagtgc acttgtagcc attacattga
     3601 tgattgcaga gtctagaccg gaagaaaagg aaacgatggt caatcttgta atgaattttt
     3661 tgaaatgtga attttgtgta aattaggatc tattgatata gtgaggattg ttcaatattg
     3721 acaacaattc ataaaatcag tagaataatc atcagttagt catctctaac aagagatgac
     3781 ttcttttata gaaaacaagt tagatatatc taactgtgga ggcataatga aaaagattct
     3841 atatattatg attggatgca tatcattagg attaggtatt attggtgtca tattaccgat
     3901 tcttccaacg gttccttttg tgttacttgc tgcattttgt tttgcaagat ctagtgaaag
     3961 attggatggt tggtttaaaa atacgaagtt gtatagagag aataatatta agaatggtat
     4021 gacgaaacaa gctaagattc gtattatgtg ttcagtaact ttattgatga gcattggatt
     4081 tattatgatg ggattaaagg gattgtgatt ggtaatattg ttttactcat tgtttggatt
     4141 tttcatatgg cttattttac agttggagtg aagactgtat gaaaagggtt ttgaattatt
     4201 cattggcaat tagctttatt cttacaatca tggttccaat cacgggaatc attgttcata
     4261 aattggcttc tacattattc ttggtattgg ctttgattca tgtcttcata tatagaaaga
     4321 gattggattt gaagtatatt cttattattg tattaaccat agtatctttt gtattaggaa
     4381 tactaggatt agtattggtt gagtatccaa taattctaac tcttcataga accgtgtcga
     4441 ttgtattaat aatcttatta gctattcata tatataaatt tcatagaagg cttgccattg
     4501 cataatgtga cggcttttat tatattcaaa gtaatggcaa atatgatgac ggatgagttt
     4561 gaaaaagaga ttggtgtgaa gggataagaa ctctgtcgta aaatattgaa tgattcagga
     4621 tggtattata attctaagat gttttcaatt tgaattgaca aatttttggg gtaggagtat
     4681 gttctgactg aagagaaaat aagtaaacta aaacaaatgt attacagttg aatttggaga
     4741 tgatattttg tattcagaac tagaatattt aaattggata tttcgtatcg tgttggctac
     4801 tttatgtggt tgtgccattg gatatgaaag gactagtcga aataaatcgg ctgggattcg
     4861 aacgcatgcg attattgcac taggatcttc aatgtttatg attatttcta aatatgcgtt
     4921 ttttgatgtg tcgacggcgg atggagctag agtggcagca caaattgtta gtggaattgg
     4981 atttcttggt gctggcgtaa tctttgtgaa agatgatttg attcaagggc ttactacagc
     5041 agctggaatg tggactactt ctggcatagc tgcatgtatt ggtgctggat tgatttttat
     5101 tggtgttttt gcgacattat ttttagtcgt tcttcaaaag atatttcatt atcctttttt
     5161 caatcaaatt gatcatccta atgtattgat tcagactaat attgtttcta agaatgatga
     5221 aatatataaa atatttgaaa agtatggtat agaggtcaat gaaataaaaa ttgataggaa
     5281 tctagattta atgacttttg atacaaatag taggtggatt gatcataaaa acgaattgtt
     5341 tcaggatttg atgtcaattg acacagtaac gaaagtatct attctctaat tgataaaggg
     5401 actatggcgg attattttga tgatgtgaat catcagatgg aaaaattatt gagaggattg
     5461 aaaatcatga agattaaaga tgtaattgaa attctaaaac aagagggtac ttgggttcgt
     5521 tggaatcgtt gtactagaga tcgtattttg tttggaaatg aaaatcaaga agtaacaaag
     5581 attggtgttt gttgggtggc tacaaataag gtgattgaac aggcattaga aaaggggatt
     5641 aattttatta tttctcatga gaatattttc tatactacgg gtacgcattt ggaaactaaa
     5701 ttagtagagt ctatagaata taagaaggaa ttattaagta agggaaatat ttgtgtatat
     5761 cgttgtcatg atgtttggga ttctattcct aaatatggtg taagtgatgt gtgggctaaa
     5821 aagcttggtt ttgattttag aaatagagtt attaattcgt attatcaatc tgcaaatatt
     5881 ccaaaacaaa ccgtttcaca attagcaacg catgtggcaa atgtattaaa agatgatggt
     5941 gaagatggtg tttatgtttt tggagatgta aataaagaag tatctcattt agctattggt
     6001 actggtgctg gaacagatat ctttgaaatg ttagaattca atccagacgt agtcattgtg
     6061 gcagatgatg gaatcacaaa ttataaggat gctcaatatg ccattgatca tgatttgcca
     6121 atgattgtag taaatcatgc gggttgtgag attggtggtt taaagaatat ggttaattat
     6181 tttaatgata aacttcctga attagatgtt gagtatttgg atgaggggtt taaaatttct
     6241 tattttaaat aagcaattgg ttaggttgaa atgatgagtg atgaaaagtt agagtggcgt
     6301 tgggctttgg ttggtaatat tgttgaaaaa catgagtatg gtgagaatca tgaaattcgg
     6361 tatggtgcta gaaaatttcg tggtggtact cgagttgttt tagctcctcc ttgttggggc
     6421 gatggatacg aacaggtaaa tgtgattggc ttgtctagat attctaggaa gtataaaaag
     6481 tccgtgattc gtagaaactt aattactaat tttagagtta agaagatata tagtcctacg
     6541 atactaaaaa taatggaaga aacagagaat aatgatagtc attgttggtg gtgggataat
     6601 acggatgagg cttatcaaga tattgttagg ttagcgaata gcttaaattc aaaagaggca
     6661 gattagtctg cctttttgtg tggaaaattt atttttttaa cttatctact tcttcataga
     6721 ataatgttcc atttagtttg tttacttgga ttgtcttttc tatccataat cttaatacgt
     6781 cagatgctgt tgagatttcg tctagtttag tactgattag agcgaagtct ggaatttcat
     6841 catcggatat tttaccgtcc attgtgattt gaatcaatcg atttactaat ggttgaatgt
     6901 catttagtgt tgcgactgta cttaatataa tgcttggtag atcttgtacg tctacttgtg
     6961 gaatgtattt ttgtccaatt tcacatttgt gtgtacagtg atagttacat agttctggtg
     7021 catggtataa atcggctaat tgtagaacga tgtctggtgc agctgtttgt ttgccttttt
     7081 caattcggta gattttgtct ggtgaaatgc catttagttc ttcactggca ctttctctgg
     7141 tatatccagc tttaactcga tattcttcgt agggagtttg aaactccttt ttgttttgct
     7201 cactcattat tatgccctca tttcgtgttt tcgcaaaaag tgcgaaaaag tgtgtttttt
     7261 tgcgcattct tttgtctgta gaaaatgctc taacaaaatt atgatataga tgcttgaaga
     7321 tatcaagaag aaagattggt gttagttatg aaaactgttg ataatcgttt acgcatgatt
     7381 cgtttaatag agaagattga aagaaataga aagtttgcgg accagatgca tgtaaaggat
     7441 gtatcggagt ttaaaaagca gagtaatgct aataagtagg aggaaaagaa aatgatgaat
     7501 agagaaagtt tattaagtag tttgaaaggt ttaagtaaga atgtttattc tagagaagaa
     7561 cttcttccgg agatggatct tttagaagaa gagatggctc agattgtgtt tgaagatggt
     7621 ttggatattc ctagtgaata tgttcatatg tcatatatta tgaaacgttt gcacatcatg
     7681 aataatgagg ctggaaatgt agcggatcaa gaattgaaga agttgtctag attggcttct
     7741 atattttcaa atcatgttcg tgctcaacag tctgggatta ctggtgaaga tagggttgaa
     7801 caatatttgg attatatgaa atccaatcat gttgttctaa gaaatcttca gttgtcagat
     7861 ggtgtttaca agacagagct tgattttctt gtattgaagc agggtgtggc aacaattgtt
     7921 gaggttaaga attctaagcg agatgtttat attgatgagg ctggtgacat gtacaaggtt
     7981 ggtagatatg aaaattttga tagtcacttg ggtgctaaga tggattttag agcggaaatt
     8041 attcaacaac ttcttaagga tgctggatat gagaatatga gaattgaaaa ggttgttgta
     8101 ttcacaaaca atcgtattca aatcaagaag gattatcatg gatttagagt ttgtttctta
     8161 tctagactcc ctcatttgat tgatgatttc tatggtactg gagttattca gtttactaag
     8221 catttacagc agatggctga ttatatccag agcaaagatt taaatgagta ctatcctttt
     8281 gaaatggatg tacaagagtt taaagaagct tttgtggatg catacctaaa aatcaaaggt
     8341 tatgatcaac cagttgaaga tggtttattt actcgtttta aaacagtaat tcacagttta
     8401 ttcaacatgc ctactacaca gcatgtttca tattaaaaat aggaggaaaa caaaatgaat
     8461 tatttagatg acatagatta tgaggatgtt tcttttatta aaaagcatcc agagattaag
     8521 ggagttttta ttacgatagc tgtgttggtt atttcttttt tgagcattgg aatatataaa
     8581 cttgcgaatc caccaaagga agaggttaat actattaatg ttgagattga gaaaaagggt
     8641 aagttgattg ttgctgaaac agaagctcat gatacagatg ttgctacatc acaagctatg
     8701 attggacctc tttcattgtg gtatgacaat tctgttgagt atgattgtta tgcgaaatct
     8761 actgttactt atgatttgga ccagactaag gctactgcta atacattaac taaggtggta
     8821 actgttaagg ctccaaagca agaggtagaa actcatttga tgcttgatac ttataagagt
     8881 attaatgaga atcaaactgt tttcaatcca gtagatgata aggatttaaa caaatctcaa
     8941 aggaaacttc agaaaagatt gaatgatgcg gctatgaagt ctgagttgcc tcaagaagct
     9001 gaaacttcat ttgaggaaca catgactaaa tggattacgg atcaggatgc ttacaaagat
     9061 tacacagtta agtttaaata tgcatagtca attggccata catattttaa tcaacagtat
     9121 ttggtaagga tggttagaaa aggaaaaaag tattgacatt atttattatc aagattataa
     9181 tgtatgcgaa tacaggattg ttactctttg aggctttacc tacatgtgtt tttggattgc
     9241 atgtttgcga agcttcgaag agttttttgt ttataaggag ttgctgttat gttaaaaaat
     9301 ctattcaaag tacaaaaaaa gattcaaata tattctccgg ttaatgggaa gtgtgtagat
     9361 ttaaaagatg taaatgatca aatgtttgcc gaaaaaatta tgggagatgg tattgcattt
     9421 aaatacaatg aaaatactat atattctccg tgtaaaggta aaattgttgc agtggcaagt
     9481 actaagcacg cattaggcat agaaacagaa gatggtattg aaattttgat tcatgttggg
     9541 ttagaaaccg ttgaatttaa tggaaaggga tttgaattgt tggttaatca aaatgataaa
     9601 gtgacaacag gacagccatt gttaaaaatt gatagagatt tttttgaaac acatcatact
     9661 gatttgacaa cacctgttgt tgtgacaagt aaaggattga atatagaatt cacaaataaa
     9721 gaggaagtaa ctataaaaga tgttgtaatg atgataaaca ggtgataaaa gatgaaagtt
     9781 gttaaatgca ttaataataa cgtagctata tgccttgatg atgataataa tgaattggtt
     9841 gcttttggaa aagggatagg gttcaaaaag cctccttttg aaattgatgt agccgttatt
     9901 caaaagacat attatggtat tgatgaaaac tatgttcata tgataaatga agttcctgaa
     9961 gaaattttgt tactctctga agagattata aaatacgcag aaaatgaact ggattatatc
    10021 tttagtccta atattatctt tacattagct gatcatatca attttgctat agtaaggtgt
    10081 aaggaaaaat taaatataac tcttccgatt gttcaagata taaaattctt gtatgaaaat
    10141 gaatatgcta taggaaaata tgcgttgtct ttgatagaaa aaagattaaa tataaaatta
    10201 ccattagaag aggctagttt tattagttta catattataa atgcagaagc cagaaaagca
    10261 ggagaaatag atggctatgc agaaaataaa gtgattatta gtgaaattac aaaactgata
    10321 gaaaataaat tcgatataag tattaacaaa gatggagcga attattcaag atttgtttca
    10381 catattcaat acttattaaa acgaattgga agtaaggagg aaataaaaag tgaaaataaa
    10441 gagttgtata tgacaatgaa aaactcatat ccagatactt cagattgcgt agattcaatt
    10501 tctgagtatt tatttgaact ttttaaatgg cgattgtcaa ttgaagaaaa actatacttg
    10561 attttacata taaatcgatt atgttcacga gaggattgtt accagtaggg cttaacctag
    10621 aacacaaatc aaattgtttt ttgattatgt tttaggtttt ttttgttaaa tgaaattaaa
    10681 ataggcgcct tttaatggca tttaacacat atttatagga ggatgatact agagaaaaga
    10741 tattgttgat aacttatttt atgaatcgaa tttaatcgta tacgacatta atgaattttt
    10801 aaattgtgaa aggagaaaaa taaatgtcaa attattctaa aaatgcagaa gatattatta
    10861 aatatatagg tgaaggtaaa aatattgaag aagttaagca ttgttttacc agactgagat
    10921 ttaaagtaaa agataactca aaagtaaatg ttgaaatgct agaaaaaata ccttgcgtag
    10981 ttcgagttat gaaaacaatg ggtcagattc aagttgttgt aggaaatgaa atagatgaag
    11041 tttataaagc tgtagaaaaa tacttggaag gaacaagtat agatgtttcc aaattagaag
    11101 aaccagtaga aacaaaagaa gaaaaaggac ctaaaggaat taaatattat ggtagtcaaa
    11161 tattaaatgc aatttcacaa actatcagcc ctattttgat gggaatggta gttgctggat
    11221 tatttaaagc aatattaact attttaactt tgacaaatat tgtaagtaca gacagtcaaa
    11281 cttatacttt gctaaattta atgggtgatt cgttcttcta ttttatgcct gtattagttg
    11341 catatagtgc atcaaaatat ttcaaatgta atacggtatt aagtttaatg ctagctggta
    11401 ttttacttca tcctgatttc attaatttag taaataatgg agaaagtatt agtttgtttg
    11461 gtctacctgt tcatgctatc tcttatagtt cttcattatt gcctgtattg tttactgttt
    11521 gggttcagtc aaagcttgaa aaactagtaa gtaagacatt tttcactaaa ttaggaatgc
    11581 tatctttatt cccaacgttt ttaattatgg ctccactaat actggttgta acaggtccat
    11641 ttggttcaat tgtcggagaa ttgattgcaa atgcaatgtt agtaatttat aacaaatatg
    11701 cagtattggg tgtatttgca atttgtttct tgatgccatt atttatttgg acaggaagtc
    11761 attgggtttt tatgccagta gcattatcaa atatgacaaa tttaggattt gatccattct
    11821 tatggcttgg atttactgca tggaactttt cacagttagc tgtatcaact gcaattatgt
    11881 tgaaaacaaa agataaagat ttaaaacaaa tcgctggttc tgctgcattt tctattgcaa
    11941 ccgcaggtat ttctgaacct tgtgcatatg gtttaacatt aaaatacaaa agaccaatta
    12001 tcccttcttt tattggatgc gctgtaggcg gattattctt tggaatcttt aaagttaaag
    12061 tattccaaat gattaatgtt tctcttttaa gtttgcctca gttcttggat ccagcaggtg
    12121 gaaacaactt tatgcttgct ataattagaa tagtattagt atttactgta acgtttgtat
    12181 taacttggtt ttttggtttt gatgataaga aagatttagg caaataggtg attatatgaa
    12241 aattaatgat agaatttatt ggatttctgg aggaccttgg ggatatttag gtaatgcata
    12301 cgctattaaa cattcaaatg gttatgtgtt agttgataca ggaagcccag aagcactaga
    12361 tgtaattgtt aaaaatttaa aatattggaa tattgatgag aacaaaatta cacatatatt
    12421 aataactcat gcccacgacg atcatactgg atgtgcacat tattttcaga aatgtggtgc
    12481 aaaaattgta atgggaaaag aagatgctaa aatgctagaa gatggtcatt taggagaata
    12541 tagtccttgt acaaatcatg ttatgccagc ttgtaaagct gatattttga ttgataaaga
    12601 tgaaatatta cagattggtg acttagaatt tgagatatta tgcttgccag gacatacaga
    12661 tggaacatta ttatactttg ttcgaattga tgatgaaaat gttatgttta gtggagatat
    12721 gttttataca gatggaaaat ggggagaatt tacaagaacc ggatggaaag gagacttgtc
    12781 ttataactct aaaaaattag gcgaatcctt tgaaaaatta tataaattaa aagaagaacc
    12841 ggatcttgca ttggctggcc acggaattcc tcgatttggt aaagaggcag aagtgattcg
    12901 tttagcttat aaatactatt tgaaatttga tagatagaat ttataatgga gtgctggatt
    12961 taaatttaaa agatattatg ataaaagcaa ttatatttga ttttaatggt actatgattt
    13021 ttgatcaaga aattttagaa aaatcatgga cattattttt aaaaaattta atacatagaa
    13081 aaccaactga tgaagaattc tccaactaca ttcatggaag aaatatagaa gagagtatac
    13141 attatttttt aggtgatgat gtagaaaata tcaatacatt aaagctagag aaagaaaaaa
    13201 tatacagaga tttttgcaag gcagacaaag aaaattataa gttggttgac ggcttgccag
    13261 aatttttaga tgatttaaaa atgaaaaatt atgtatgtgc tattgcaaca gcatcgggac
//
LOCUS       DBMDWL010000043        13287 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1587_length_13287_cov_4.100258, whole genome shotgun sequence.
ACCESSION   DBMDWL010000043 DBMDWL010000000
VERSION     DBMDWL010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13287)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13287
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1587_length_13287_cov_4.100258"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="ACZPDN_04490"
     CDS             complement(<1..500)
                     /locus_tag="ACZPDN_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024502.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase central domain-containing
                     protein"
                     /protein_id="HIR1297912.1"
                     /translation="MAVKKDEKTGTWYFYGSYKMKNGKYRQYKKRGFPKKKDAVKAEI
                     IFKENVKDPYKNITLEELFNVYAAYTEKRIKESTYKVQNRLLERWIDILGDVNIKSIT
                     THDIECAMELMINNVGYETAKNYLSRINKMLRFAVRKGYLESNPCSPVELAKNPNEKK
                     TEMQYWT"
     gene            complement(1459..3150)
                     /locus_tag="ACZPDN_04495"
     CDS             complement(1459..3150)
                     /locus_tag="ACZPDN_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1297913.1"
                     /translation="MNINYVQSGEPYGGQCVSLIQGLMAWGGKPCVPRGHARDWWFNR
                     ASNGVLSYFDVVTGAPQNGDVGVSVGGDARYGHIFIYWEGRALSQNVLGNPKAMLWPL
                     NYQGAIWGYLRPKFYTNASTYDASQLIKENGMATFENDTAIVIHRDTPTGASYGTFVK
                     GEKQVYTEKWIGNGHRWISWIHTNGVRCFSAVSGSESYGVEPWATISAPETKDIELTQ
                     EDGIAEFIVDGVHKHYDNPSGEIFGQCNSGDKIRYYWKCVTNGHRYVVGKEGDRKFFV
                     AVSATEDRSQMWAKFSAHDTNTKEDTKEPSKPSTEPSKPITTDYTKNVKGYGIDVSEH
                     NSSDIDLSKYDFVILRASYGEYTDKKFEYFADKCEQLKIPYGVYVYDYALDDSQAREE
                     AEYAYNLIKDKNVQLGVWFDMEDADNYKKKAGVLTKERCSFSCKVFCDYMSAKGYYTG
                     VYTSTSWLGTFVETTYPIWIANWGTNDGNIQSDQSGVGVIHQYAANPIDKDIIFHDID
                     FYKSNPNKDESTDDKKDEPNTDSKDDNGNKINVTGINKLIELLLKIVEKIANLFK"
     gene            complement(3229..3630)
                     /locus_tag="ACZPDN_04500"
     CDS             complement(3229..3630)
                     /locus_tag="ACZPDN_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297914.1"
                     /translation="MGKVITIDLEYVLWLLGFIASAWGVLKIVKEVKKPNDDLKETVR
                     RHEEWLVRDNDRIKSIESLVITQEGIKKELNEHSRRLGEHEERLEEDKQRGNLTLKAN
                     IAIINNMLSENDKDKLQETRDEIQNFLLDKN"
     gene            complement(3630..4010)
                     /locus_tag="ACZPDN_04505"
     CDS             complement(3630..4010)
                     /locus_tag="ACZPDN_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297915.1"
                     /translation="MNVQDFLSLLQTAATLVCGGLALYFKFSTKAKTKAKEVQEVIAK
                     ITAQAVVYIKEAEDNYKDTTNAGGKKFEEVVGKLYYLVPDALHGIITKEMISEIVQST
                     FDEIEEYVKIQLDNGIDKINFKGE"
     gene            complement(4228..4371)
                     /locus_tag="ACZPDN_04510"
     CDS             complement(4228..4371)
                     /locus_tag="ACZPDN_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297916.1"
                     /translation="MYRILNNQKSRVIDGKYSKDNYIFLVEQAYKKKKITKAEYQELI
                     DFE"
     gene            complement(4352..4549)
                     /locus_tag="ACZPDN_04515"
     CDS             complement(4352..4549)
                     /locus_tag="ACZPDN_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297917.1"
                     /translation="MIIKQLDVSIEKQAQEEYQASQVQSTKDELANQKFLTEYVACMA
                     GIELPVDEEETEGMNHVQDFE"
     gene            complement(4546..5589)
                     /locus_tag="ACZPDN_04520"
     CDS             complement(4546..5589)
                     /locus_tag="ACZPDN_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HIR1297918.1"
                     /translation="MKIKEYITDYDQLFDSMSKCKKNVSWKPSVKSFVLNGVENCLKM
                     EEQLQNDTWINRKPKPIVVTYPKRRECLSIPFRDRVYQRSINDNSLYPQTTKHFVYTN
                     IACQKFKGTKKAMDVMRQYLHRYYINNKTNVGYVVWIDIHGYYQNMRHKDVNECFYKM
                     CDSDTASMSKDVLDTQYSGDIGYNPGSQMVQIAGISLLNELDHFIKEKLHCKSFIRYM
                     DDSYLITNDKEKAKQWKKIVCDKLIELGFEPNPKKAKVLRIDKGFMFLGFKATLSNTG
                     KVYYNLSSENIKHERRKLKKQVIKAKKGEMTKEEIDASLHSWKSHAELGNTYKLLQRI
                     DAYYANLWKEIKV"
     gene            complement(5944..6387)
                     /locus_tag="ACZPDN_04525"
     CDS             complement(5944..6387)
                     /locus_tag="ACZPDN_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297919.1"
                     /translation="MATNVNERNVPDTPTNKMLDCLWEARNLSLYTVKICSNKNNFPP
                     EYYQTMTGDMIKKAKDIYRLGKRANAIYVKGKTGHERWEERSRYQREAIFLCVDLLSD
                     IDVAKTLFNIRGKRVKYWTKQVVTVKRMYIAWHNADKERYAKYIN"
     gene            complement(6442..7626)
                     /locus_tag="ACZPDN_04530"
     CDS             complement(6442..7626)
                     /locus_tag="ACZPDN_04530"
                     /inference="COORDINATES: protein motif:HMM:NF040368.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HIR1297920.1"
                     /translation="MAKHKILTDETGEKIVKALNIIAQNGISHQSMDWQKVRTLIANG
                     VGESAFAIGTQLIEKWTDTADSKEYDMPWQVNHFEDITLEDGEVVPGMWLQTHYTLPF
                     DIQFSHQRAFLACPDGLSAGTYNFDFAKAWGNNVKPGINYQFTLTKPVERGGRLAGCY
                     GAPDQAPSNWKVYSYGKDGITLNETVNVTVGSDGTNLGTIQHDSRSGNLNSVQEMAYG
                     WNRWKTSALRQYLNSSKPKGQWWTPQDQWDICPDQLASKDGFLCGMPEEMLNCLKKVK
                     VVTYANTVNDEGAEDITYDYVTLPSLSQMFIKPQTSGEGDVHTYWKRRSGRTTPCEWW
                     TDYPNMVEYSVANKTSPQYVRLRSANQGYACYAWSVVTSGGVNGSYASSAYAFAPLVC
                     IA"
     gene            complement(7626..7832)
                     /locus_tag="ACZPDN_04535"
     CDS             complement(7626..7832)
                     /locus_tag="ACZPDN_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297921.1"
                     /translation="MGVRIAELPSSEGISKTDLIIVQDNESTKQGTIQQLDDSLGVSR
                     LKEEFEALGLSVDEEGYIVQEVQE"
     gene            complement(7849..9339)
                     /locus_tag="ACZPDN_04540"
     CDS             complement(7849..9339)
                     /locus_tag="ACZPDN_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1297922.1"
                     /translation="MAYVGYNWALLANHQIWSYSGRCNMYFQVYAWSEQDVINNRSTV
                     HTRTRILVENKNPSYSGYYVEQDWSAGVTGAPNYSAHATFSDGGAGTSKEYILQNGSF
                     TVNHDSNGNASSKIHYWFNGTYTGAIGSPTNTNVVDISLPKIDRTADKAKISNVGSTY
                     KTMYCTISVPFYSDENQWSRDGKTWTDWNKVIKADTPFVDTWTGLKPNTKYTGYYRFK
                     RKYNGVWSEAVDFTATTKYPNAPSKGSVSLSSVTSNSAKVSWSGFSLGEMATDYSYQT
                     SNDENNWTDQGKATSLTLSDLKPNTNYKFYVRMVDNYGQPSLAASTSFTTLNPEKPNV
                     GGIECTRLTPFGGMFSWYGFSVNEGATVDHYEYSLDNSNWINVGTDTQINLDNLSPET
                     SYTLYVRIVDNFGSKSDSATFSFKTLVDQLKIAYNTNVYQTEILTKDGVDILAKNGDN
                     LIVDTIGKERLMTAKVFYNENGVIKKVKSAYYNKKGNIQHYKNYGN"
     gene            complement(9343..10005)
                     /locus_tag="ACZPDN_04545"
     CDS             complement(9343..10005)
                     /locus_tag="ACZPDN_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297923.1"
                     /translation="MQPVVCNKDMAVVFPLKDGDCQFWLEIVDSVDDITNPSRDHAYV
                     DSKGLFYIYNGKEIQVINDHANLKIKWGNMIGDISNQLDLIEILNQFVKTISVNGTNI
                     AKDNEKNIAIQVPITTIKLDGNTINPVDYIVNLDLASVYAKKTEIPKNVSELENDAGY
                     IKQEVVDQLVPIKTIKVNDVTIQPDENHAVNIESIRYKVGTADPNTTNCPNGYFYFQI
                     GD"
     gene            complement(10007..10474)
                     /locus_tag="ACZPDN_04550"
     CDS             complement(10007..10474)
                     /locus_tag="ACZPDN_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297924.1"
                     /translation="MENKYSTSGIGEDIIRSFTQIASAELHAKTLLEKRISEVENGLI
                     SEEEIPDNLEKIEALKDEIDDYANIRRSQMLYLYNSFGGKGDREQWCLVKHLSMSMYT
                     AFEAYQASDRDPELLNIALEINKKFIEACTKFLGVEITSCASCFADIMKAGGK"
     gene            complement(10475..10654)
                     /locus_tag="ACZPDN_04555"
     CDS             complement(10475..10654)
                     /locus_tag="ACZPDN_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297925.1"
                     /translation="MNRYEQRIENLSNHVKQNPRDWQSAISLLKLNNQQIDFKRKQKQ
                     QSARLSIKAYKKEVV"
     gene            complement(10664..11158)
                     /locus_tag="ACZPDN_04560"
     CDS             complement(10664..11158)
                     /locus_tag="ACZPDN_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297926.1"
                     /translation="MDISKAINILADSVYDLKEKGRYNSIQRRNHTVDFYGYEFPRWG
                     CSSSKPAVIGMSISQDLIYYERFEFKLVIDNSTATNFNIEIEGIDMTPYFKQQFNGAW
                     ITGNGLWPGQYSNFDVLKACGYLSETDRNRILDPGYKTIKVTGNGNFDCTLVNYLKYS
                     HVNR"
     gene            complement(11161..>13287)
                     /locus_tag="ACZPDN_04565"
     CDS             complement(11161..>13287)
                     /locus_tag="ACZPDN_04565"
                     /inference="COORDINATES: protein motif:HMM:NF037025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HIR1297927.1"
                     /translation="VIMDCGVFYGMITDYKPSVSGLNISLTHVINEWIYRQVPTNYAV
                     KNALIKNVYESEDMYYSTQWKMNFETEIDNEKIDYVYSRQSKLDALTKTCELTPSVYW
                     RVPFTNDKQVEVGYFGKKQPVMLSNKPTLGRNYRIIGEPTMETDFSDVINLATVYANK
                     SDSGMSSLSLREVYNDKSLQNPKFPVVILRSNINNERDYEYVDFPKLAPNNQLEYSII
                     DTESVGYESGVFIEGTFAFDDLSPFSLEDMTKDSKDYKWVIPKEQRYLNDTEEINNAK
                     ALWHSLKDIWSKSAIAALCGSCHVESTLNPNLYQMGDVPDSQKGFGLVQWTPYTRITN
                     WLGSHGYTSYTMYGKGEVAKLVEEWSTNATNGPWIPTSSYNITFQQWSHMEADMNYMV
                     MAFMADYERGDTSIDLQYQKRIEFAQRIYGLIHEWEQDDNGTTTDTDKTQSRPWNAQN
                     FINTWNGQSIDMDGVPIEQPYQCVDAWKKALQTLNYPDPTRAIGGDGYADYIWYNRDE
                     LGYSQYFDYVSTPQFGDWCIFGRSGDTPASHVAMYVSDAGNGRANFFGQNQPYPYCNT
                     TTISTSNIIGIFRVKSVYVQQSIDPESTNGTTIITDNDRIYAAKVVYDCACRKLINAR
                     RKFAINTSCEALPKEVNVGDRIRFVYDLNLLQLGSCNRYMKRILKQDDWFYITSLQRE
                     IDKTGIEIDTLTLEKFLRTDRYGKSE"
BASE COUNT         3865 a         2621 c         1880 g         4921 t
ORIGIN      
        1 gtccaatact gcatttctgt tttcttttcg tttgggtttt tagccaattc aacaggcgaa
       61 caaggatttg attctaaata tcctttacga accgcaaatc tcaacatctt attgattcta
      121 gataaatagt tctttgcagt ttcatatcct acgttattaa tcattaattc cattgcacat
      181 tctatatcgt gtgttgtaat ggattttatg ttcacatcac ctaaaatatc aatccatctt
      241 tcaagcaatc tattttgaac tttataggta ctttctttta ttctcttttc tgtatatgct
      301 gcataaacat taaataattc ctcaagtgta atattcttgt atgggtcttt cacattctct
      361 ttgaatatga tctctgcttt tactgcatcc ttcttttttg gaaagccacg tttcttatat
      421 tgtctatact ttccgttctt cattttgtac gaaccataga aataccacgt acctgttttt
      481 tcatcttttt ttactgccat gtaattttcc ctctttcttt agataacact taaattttat
      541 aaaaaactag tgaaaatagg tgaaaaataa gtctatttta tgccaatatc atgccaataa
      601 tatgtatcat gctttatata aagcaattat ctttttgttg aatgatattt atatgtttct
      661 aggtctttat ttttctttat tttttattca tctagtttat tttatgttct catattttct
      721 cattttttac tacacttttt ttaattgaac ataaaaaaat attccatacg ttatgccacg
      781 cactttatgc cattatgcca aaaccatata taaacaaaaa agcctcccgc ttggtaagga
      841 gactcttttg cataaagtta tcttagaaag ggttgtgtcc atccatgaag aacacatcaa
      901 taatatatca cataaatttt aagatttgtt aaaaaacaaa aaccatacct ggatgtgtta
      961 ggtatggaat cgtttttgtg acagtttata aaagggggag cttttagcaa ttgtcacatt
     1021 ttgtagttgt ttgctagtgc cgccatgaga aaattgaaga tacatcacac gtctgcaatg
     1081 ttatctacac aaaagagaga ttcggccttt atcactcttc tttttactag caacatgatt
     1141 atatcatgaa cacaaatatt tttatgcata aaaaaagaag aatacgattg tattccccta
     1201 agcatagata ttataccata ttatggtatt ggtcgtattg tgcactctac taatctatgt
     1261 gcatatattt taacataaaa agcaaagacc gtacaaacat catacggtct aagaaactcc
     1321 ttctacacta gtagacgaga tattttcgtt cacgatagtt cacactacaa tgaatgattt
     1381 acaacatata aacctctttt tttgcagatt ttgtgcaaaa acaagcattt catgtcgcat
     1441 ttcgtacatt atgtaaaatt atttaaacaa attagcgatt ttttcaacga tctttagcaa
     1501 cagttcaatc aatttattga ttcctgtaac attgattttg tttccattat cgtctttaga
     1561 atctgtattt ggttcatctt ttttatcgtc tgttgattca tctttatttg gatttgactt
     1621 ataaaaatca atatcatgga agattatatc tttgtcgatt gggttagctg catactgatg
     1681 aataacacct acaccagatt ggtctgattg aatattacca tcattcgtac cccaatttgc
     1741 aatccaaata ggatatgttg tttctacaaa tgttcctagc caactagtac tagtataaac
     1801 acctgtataa tatcccttag cactcatata gtcgcagaat actttacaag agaaagaaca
     1861 tctttctttt gttaagacac cagctttctt cttgtaattg tcggcatctt ccatatcgaa
     1921 ccatacacct aattgaacat ttttgtcctt gattagattg tatgcgtatt ctgcttcttc
     1981 cctagcttga ctatcatcta atgcataatc atatacataa acaccataag gaatcttcaa
     2041 ctgttcgcac ttgtctgcga agtattcaaa tttcttatca gtgtattctc cgtaggaagc
     2101 acgcaaaatc acaaaatcat atttagataa atcaatatct gaactgttgt gttctgaaac
     2161 atcaattcca tatcccttaa cattcttagt gtaatctgtt gtaattggtt tagaaggctc
     2221 tgtagaaggc ttagaaggct ctttcgtatc ttccttagta tttgtatcat gagcactgaa
     2281 tttcgcccac atttgacttc tatcctctgt agcagacacc gcaacaaaga actttctgtc
     2341 tccttcttta cctacaacat atctatgtcc atttgttacg cacttccaat agtagcgaat
     2401 cttatctcct gaattacatt gtccaaaaat ttctccactt ggattatcat agtgtttatg
     2461 cacaccatca acaataaatt cagcaattcc atcttcctga gttaattcaa tatcttttgt
     2521 ttcaggagca ctgattgtgg cccatggttc aacaccatat gattcactac cactaactgc
     2581 cgaaaaacat ctaactccat tagtatgaat ccatgaaatc catctatgcc catttccaat
     2641 ccatttttct gtatagactt gtttttcgcc ctttacaaat gttccataag aagcacctgt
     2701 tggtgtatct ctatgaataa cgatagcggt atcattttca aatgtggcca ttccattctc
     2761 tttaattaat tgagaagcat catatgtaga agcatttgta tagaatttag gtcttaaata
     2821 tccccaaata gcaccttgat agtttaatgg ccataacata gctttagggt ttcctagaac
     2881 attctgagag agtgctctac cttcccaata gataaatata tgtccgtatc ttgcatcacc
     2941 gcctacagat actccaacat caccattttg gggagcacct gtaacaacat caaaataact
     3001 taacacacca ttgcttgctc tgttaaacca ccaatcacga gcgtgtccac gaggaacaca
     3061 tggctttcct ccccatgcca ttaagccttg aattaatgaa acgcattgtc cgccatatgg
     3121 ttcaccactt tgaacataat taatgttcat tatttgccct ctgttgttaa aaactttatt
     3181 gagagcatag ttataaaaat cttgtggagt tcccattatt taacctcctt aatttttatc
     3241 tagaagaaag ttttgaatct catctctagt ttcttggagt ttatctttgt cgttttcaga
     3301 aagcatattg ttgataattg cgatatttgc ttttaatgtc aaattaccgc gttgcttatc
     3361 ttcttctaat ctttcttcat gttctccaag ccttcgagaa tgctcattca attctttctt
     3421 gataccttct tgcgtgataa ctaaactttc aattgatttt attctatcat tgtctcttac
     3481 taaccattct tcgtgtcttc taacagtttc ttttaaatcg tcattaggtt tcttcacttc
     3541 tttaacaatc tttaatactc cccaagcgga agcaatgaaa cctagaagcc ataaaacata
     3601 ttctaaatca atagtgataa cttttcccat tattcacctt tgaagttgat tttatcaatt
     3661 ccattatcta attgaatctt aacgtattct tcaatttcat caaaagtact ttgaacaatt
     3721 tcactaatca tttcttttgt tataattcca tgcaatgcat caggtactag ataataaagc
     3781 ttaccgacaa cttcttcaaa cttcttgcca cctgcattag ttgtatcttt gtagttgtcc
     3841 tctgcttctt taatgtatac aactgcttgg gcagtaattt tagcaatcac ttcttgaact
     3901 tcttttgctt tagttttagc tttcgtactg aatttaaaat ataaagctaa tccaccacaa
     3961 actaaagtag cagcagtctg taataaagat aaaaaatctt gtacattcat aaatttacac
     4021 ctccaaaaat atttcatctc tctcatattt tctgaggtac tgttttatgg catatcaatt
     4081 atataacgaa aagaaaagga cgtacattat caccctttct ctttgtccaa aacattaatt
     4141 ccattcatat atagaatctc attctgttct ttgatgattt tattttgctt atcaataata
     4201 tctaataaat attgaatata ctcgaaatta ctcaaaatca atcaactctt gatattctgc
     4261 tttagtgatt ttctttttct tataagcttg ttcaactaag aaaatataat tatctttgct
     4321 atactttccg tcaatcactc tgcttttctg attattcaaa atcctgtaca tgattcattc
     4381 cctccgtttc ttcttcgtca acaggtaatt cgatacctgc catacaagca acgtattccg
     4441 ttagaaactt ttgatttgcc aattcgtctt ttgtagattg aacttgtgaa gcttgatatt
     4501 cttcttgagc ttgtttttcg atagaaacat ctaattgttt gataatcata cttttatctc
     4561 cttccataga ttagcgtaat acgcatctat tctttgcaat aacttgtacg tattccctaa
     4621 ttctgcgtgt gatttccaac tatgaaggct tgcatcaatt tcttcttttg tcatttcacc
     4681 tttcttggct ttaatgactt gtttctttaa ttttcgcctt tcatgtttta tattttctga
     4741 acttaggttg taatatacct tgcccgtatt tgataatgta gctttaaatc caagaaacat
     4801 aaatccttta tctattctta gaaccttggc tttctttggg ttaggttcaa accctaattc
     4861 gattaactta tcacaaacta ttttcttcca ttgtttcgct ttttctttgt catttgtaat
     4921 caaataggaa tcatccatat atcttatgaa acttttgcaa tgtagttttt ctttgatgaa
     4981 atggtctaat tcattcaaca agcttatgcc agcaatctga accatttgag aacctggatt
     5041 atatccaata tctcctgaat attgtgtatc taacacatct ttagacatac tagcggtatc
     5101 tgaatcacac atcttataaa aacattcatt tacatcttta tgtctcatat tttgataata
     5161 tccatgtata tctatccata caacatatcc tacattcgtt ttgttgttga tgtaatatct
     5221 atgaagatat tgtctcatta catccatagc tttcttagtt cctttaaact tttgacaagc
     5281 tatatttgta tagacaaagt gctttgttgt ttgaggatat aatgaattat cgttaatact
     5341 acgttgataa actctatccc taaaaggaat gcttaaacac tcccttcttt ttggatatgt
     5401 aacaacaatc ggtttaggct ttctgtttat ccatgtatca ttttgcaatt gttcttccat
     5461 cttcaaacaa ttttctacac catttaacac aaatgattta acactaggtt tccaagatac
     5521 atttttctta catttcgaca ttgaatcaaa caattgatcg tagtctgtaa tatattcttt
     5581 tattttcaaa ataatagttt ccttccgtca tagttagcta ccatcttttt ttcagaggct
     5641 ctaacatcgg tggcaaaata cactgattta tgtatattct gcggtattgt tcgctctaat
     5701 gagcagggat gaggctcctt gtttacacat atcaaatcgt atcaacatct aaggttgacc
     5761 atatagttta aattgataaa caatcggggc gaacgcattc gcattagaag cgttgttgtt
     5821 gttgacatta ccgctagtgt tcacattcca cgtataacaa gcattgcctt ggttggccga
     5881 acgcaaacgg acgttctgag aatcaaccta catcccgtat gtattagtaa ttattaataa
     5941 atactaattg atatattttg catatcgttc tttatctgca ttgtgccatg cgatatacat
     6001 tctctttacc gttactactt gcttagtcca atatttaact cgttttccac gaatgttaaa
     6061 taatgttttt gccacatcta tatcagataa taaatctacg caaaggaaaa tggcttcacg
     6121 ttggtatcta ctgcgttctt cccatctttc atgtcctgtc ttacctttaa catagattgc
     6181 atttgctctt tttcctagcc tgtatatatc ttttgccttc ttaatcatgt cacccgtcat
     6241 tgtctgataa tactcaggcg gaaaattgtt tttgtttgaa caaatcttta cagtatacaa
     6301 agacaagttt cttgcttccc ataaacaatc caacatttta tttgtcggtg tgtcaggtac
     6361 atttctttca tttacattag ttgccattca ataattcctt tccttgtatg gggcagtacg
     6421 ttgtctgccc cgattttaga tttatgcgat acagacaagc ggggcgaacg catacgcact
     6481 agaagcgtag ctgccgttga caccaccgct agtgaccaca ctccacgcat aacaagcata
     6541 gccttggttg gccgaacgca aacggacgta ctgaggtgat gttttatttg caacggaata
     6601 ctcaaccata tttggataat ctgtccacca ttcgcaaggt gttgtacgtc cgcttcttct
     6661 tttccaatag gtgtgaacat caccttcacc actagtttgt ggcttaatga acatctgaga
     6721 tagtgaaggt aacgtaacat aatcatatgt aatatcctca gcaccttcat cattaacagt
     6781 attagcataa gtaacgactt tcactttttt taaacaattt agcatttcct caggcatacc
     6841 acaaaggaat ccgtctttag aagctaattg gtcaggacaa atatcccatt gatcttgtgg
     6901 agtccaccac tgtccttttg gtttacttga gttcaaatac tgtcttaaag cagatgtttt
     6961 ccatctgttc catccatatg ccatttcttg tacagagttt aaatttccgc ttctactatc
     7021 atgttggatt gttcctagat ttgttccatc actaccaaca gtaacattga ctgtttcatt
     7081 taatgtaatt ccatcttttc cgtatgaata aactttccaa ttagatggtg cttgatcagg
     7141 tgcaccatag catccagcta atctaccacc tctttctaca ggtttagtta atgtgaattg
     7201 gtaattaatt cctggtttaa cattatttcc ccatgcttta gcaaaatcga aattataagt
     7261 accagcacta agcccatcag ggcacgctag aaacgctctc tgatgcgaaa attgaatgtc
     7321 aaaaggcaaa gtatagtgcg tttgtaacca cattccaggg actacttctc cgtcctctaa
     7381 agtaatatct tcaaaatggt ttacttgcca tggcatatcg tactcttttg aatcagcggt
     7441 atctgtccat ttttcaatta actgcgtacc aattgcaaac gcactttcac caactccatt
     7501 tgcaattaac gttcttacct tctgccaatc cattgattga tgcgaaattc cgttttgagc
     7561 aatgatattc aatgctttta caattttttc tcctgtttca tctgttaaaa ttttgtgttt
     7621 cgccattatt cttgtacctc ctgaacaata tatccttctt cgtctacaga taatcccaac
     7681 gcttcaaatt cttctttaag cctacttaca cctaaagaat catctaattg ttgaattgtg
     7741 ccttgcttgg tagattcatt atcttggacg ataattaaat ctgtttttga aatgccttcg
     7801 cttgaaggca attctgcaat tctaactccc attatatgta cctcctattt aatttccata
     7861 gtttttatag tgttgaatat tacctttttt gttgtagtaa gctgatttta ctttctttat
     7921 tactccattt tcattgtaga aaaccttggc agtcattaat cgctcttttc caattgtatc
     7981 aacaatcaaa ttatctccat tcttagctaa gatgtctact ccgtctttag ttagaatttc
     8041 tgtttgatac acatttgtat tgtacgcaat cttcaattgg tcaaccaatg ttttaaaact
     8101 gaatgtagca ctatctgatt tagagccgaa gttatcaaca atacgaacgt ataatgtata
     8161 acttgtttca gggcttaaat tatctaaatt aatttgcgta tcagttccca cattaatcca
     8221 atttgaattg tctagtgaat attcataatg atctactgtt gctccttcat ttacagagaa
     8281 tccataccaa gaaaacatac ctccaaacgg tgttagtcgt gtgcattcaa taccacctac
     8341 atttggtttt tcagggttca atgttgtaaa tgatgtacta gctgctaacg aaggttgacc
     8401 atagttatcg accattctta catagaattt atagtttgta ttaggtttca aatcactaag
     8461 agttaagctt gttgctttac cttggtctgt ccaattattt tcatcattag atgtttgata
     8521 agaataatca gtggccattt ctcctaatga gaatccgctc caacttactt ttgcagaatt
     8581 ggacgttacc gaacttaaag aaacacttcc ttttgaagga gcattaggat atttagttgt
     8641 cgcagtaaaa tcaactgctt cactccaaac tccattatat tttcttttga aacgataata
     8701 tccagtgtat tttgtgttcg gctttaaccc tgtccatgta tctacaaaag gagtatcggc
     8761 ttttattact ttattccaat ctgtccatgt tttcccgtct ctactccatt ggttttcatc
     8821 agaataaaac ggaacagaaa ttgtacagta cattgttttg tatgtacttc caacattact
     8881 tatctttgcc ttatctgcgg ttctatcaat tttaggaagt gagatgtcta ctacatttgt
     8941 gtttgtaggg cttcctatag ctcctgtata tgttccatta aaccaataat gtattttgct
     9001 tgatgcattt ccattagaat catggttaac tgtaaatgaa ccattttgta gaatatattc
     9061 cttgcttgta ccagctccgc catctgaaaa tgttgcatga gcactataat ttggtgctcc
     9121 tgtaactcca gcagaccaat cttgttcaac ataataacct gaatagcttg ggtttttatt
     9181 ttcaactaaa atcctagttc ttgtatggac tgtagaccta ttatttataa catcttgttc
     9241 gctccatgcg tatacttgga aatacatatt gcatctgcca ctgtatgacc aaatttgatg
     9301 atttgctagt aatgcccagt tataacctac ataagccatg gattagtctc ctatctgaaa
     9361 gtagaaatat ccattagggc aattcgttgt gtttgggtcg gcagtcccga ctttataacg
     9421 aattgattca atattaactg catggttttc atcaggttgt atcgttacgt cattaacctt
     9481 gattgtttta ataggaacta attgatctac aacttcttgt ttaatatatc cagcatcatt
     9541 ttcaagctca gatacatttt taggaatttc agttttcttt gcataaacac tagctaaatc
     9601 taaattgaca atataatcaa caggattaat cgtgtttcca tctaatttaa tagttgtgat
     9661 aggcacttga atagcaatgt ttttttcgtt atctttggca atatttgttc cgtttacaga
     9721 aattgtcttt acgaattgat ttagaatttc aattaaatcc aattgattag aaatatcacc
     9781 aatcatattt ccccatttga ttttcaaatt ggcatggtca ttgattactt gaatttcttt
     9841 tccattgtag atatagaaca atccttttga atcaacatac gcatggtctc tacttggatt
     9901 tgtaatatca tctacagaat caacgatttc tagccaaaat tgacaatcac cgtcttttaa
     9961 aggaaatact accgccatat ctttattaca tactacaggt tgcatattat tttcctccag
    10021 ctttcataat gtctgcgaaa caagatgcac aagaagtaat ttctacacct aagaattttg
    10081 tacaagcttc aataaacttc ttgttaatct ccaaagcaat attcaataat tcggggtctc
    10141 tatccgaagc ttgatatgct tcaaatgcag tgtacataga catacttaaa tgtttaacta
    10201 aacaccactg ttctctatcc cctttgccac cgaaagaatt gtatagataa agcatttgag
    10261 aacgtctgat gtttgcataa tcatcaattt catcctttag tgcttcaatc ttttctaaat
    10321 tatcaggaat ttcttcttca ctaattaatc cattttcaac ctcagaaata cgtttttcta
    10381 ataatgtttt agcgtgtagt tctgcacttg caatttgtgt aaaactacgg ataatatctt
    10441 ctccaattcc cgaagtgcta tatttgtttt ccatctacac aacctccttt ttgtatgctt
    10501 tgatagataa tctagcagac tgttgttttt gttttctttt aaagtcaatt tgttgattgt
    10561 tcaatttcaa tagcgatatg gcagactgcc aatctctagg gttttgtttt acatgattcg
    10621 ataggttttc aatcctttgt tcatatctat tcatagatac ctcttatctg tttacatgac
    10681 tatatttaag gtaattaact aacgtacaat caaaattacc gtttcccgtt actttgattg
    10741 ttttatatcc tgggtctaat attctgtttc tatcggtttc agaaagatac ccacaagctt
    10801 taagaacatc aaagtttgag tattgtccag gccatagtcc atttcccgta atccacgctc
    10861 cgttgaattg ctgcttgaaa tatggtgtca tgtcgattcc ttcaatctcg atattaaagt
    10921 ttgtagcagt agaattatct attactagtt taaactcaaa acgctcataa taaatcaaat
    10981 cctgtgaaat tgacattcct attaccgctg gtttagaact tgaacatccc catctaggga
    11041 actcataccc ataaaaatca actgtatggt ttctacgttg aatagaattg tatcttcctt
    11101 tttctttcaa atcatagaca ctatcagcta atatatttat cgccttacta atatccataa
    11161 ctactcactc tttccgtatc tatctgttct aaggaatttc tctagtgtca atgtatctat
    11221 ttcaattcct gttttatcta tttctctttg taggcttgtg atatagaacc aatcatcttg
    11281 ttttaaaata cgtttcatgt atctattaca acttcctaat tgcaataaat tgagatcata
    11341 aacaaatctg attctatcac ctacgtttac ttctttaggt aatgcttcac aagaagtatt
    11401 gatagcaaac tttcttcttg cgttaattag ttttctacag gcacaatcat atacaacctt
    11461 tgccgcatat attctatcgt tatcagtaat gatagtcgtt ccgtttgtag attcagggtc
    11521 aatgctctgt tgtacataaa cgctctttac tctgaaaata ccaatgatat ttgatgtact
    11581 gattgttgtt gtattgcaat atggataagg ttggttttgg ccaaagaaat ttgctctacc
    11641 attgccagca tctgaaacgt acattgcaac gtgtgatgca ggtgtgtcac cgcttctacc
    11701 gaatatgcac caatcaccaa attgaggtgt gctaacgtaa tcaaaatact gagaataacc
    11761 taattcatct ctgttatacc aaatgtaatc tgcatatcca tcaccgccaa tagctcttgt
    11821 aggatcggga taattcaatg tctgtaatgc tttcttccaa gcatctacac attgatatgg
    11881 ttgttcaata ggaacaccat ccatgtcgat agattgacca ttccatgtgt taataaaatt
    11941 ctgagcgttc caaggacgag attgtgtttt atctgtatct gttgtagttc cgttatcatc
    12001 ttgctcccac tcatgaatca aaccataaat acgttgagca aattcaatac gtttttgata
    12061 ctgtaaatca atagatgtat caccacgttc ataatccgcc ataaaagcca ttaccatgta
    12121 attcatatcg gcttccatgt gtgaccattg ttgaaatgtg atgttataag aagaagttgg
    12181 aatccaaggc ccatttgtag cgtttgttga ccattcttca accaacttag ctacttcccc
    12241 ttttccgtac attgtgtagc ttgtgtaccc atgagaacca agccaattgg taattcgtgt
    12301 gtatggagtc cactgaacta gtccaaatcc tttttgagaa tcaggaacat cacccatttg
    12361 atacaagtta gggtttaatg ttgattccac atgacatgag ccacataaag cagcaatagc
    12421 agatttactc caaatatctt ttaaagagtg ccataaggct ttagcattgt ttatttcctc
    12481 tgtatcattc aaatatcttt gttctttagg gatgacccat ttataatcct tagagtcttt
    12541 tgtcatgtcc tctaggctaa atggagataa atcatcaaag gcaaatgttc cttcaatgaa
    12601 tacaccactt tcatatccaa ccgattccgt atcaataatg gaatactcca attgattgtt
    12661 aggagctaat ttaggaaagt ctacatattc ataatcacgt tcgttattta tgtttgatct
    12721 taaaataact acaggaaact tagggttctg taaactttta tcattatata cttctctcag
    12781 agataaagag gacataccac tatcagactt attagcataa actgtagcaa ggttgataac
    12841 atctgaaaaa tcggtttcca ttgttggctc tccaatgatt ctgtagtttc ttcctaacgt
    12901 tggcttatta gaaagcataa caggttgttt ctttccaaaa tatccaactt caacttgctt
    12961 atcatttgta aaaggaactc tccaatatac agatggtgtc aattcacaag ttttagtaag
    13021 tgcatctaat ttagattgtc tagaataaac atagtcaatc ttttcattat caatctctgt
    13081 ttcaaaattc atcttccact gagtcgaata atacatatct tcactttcgt atacgttctt
    13141 tataagagcg tttttaaccg cataattcgt tgggacttgt ctatatatcc attcgttaat
    13201 tacgtgcgtt agagatatgt ttaaaccact tacagatggt ttgtagtcgg taatcattcc
    13261 gtagaaaact ccacaatcca taattac
//
LOCUS       DBMDWL010000044        13102 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1609_length_13102_cov_2.091455, whole genome shotgun sequence.
ACCESSION   DBMDWL010000044 DBMDWL010000000
VERSION     DBMDWL010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13102)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1609_length_13102_cov_2.091455"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /locus_tag="ACZPDN_04570"
     CDS             <1..2049
                     /locus_tag="ACZPDN_04570"
                     /inference="COORDINATES: protein motif:HMM:NF018897.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HIR1297928.1"
                     /translation="GQSQEKIMKKEDSRNHAPGNRAADCSGFCMPMKQYNDVINLSYK
                     FAQENNMEVVPIDLQLSEDKQERTKQFNQVYSFLQEHSYPCFVGIVSKNIKTEYVYLP
                     KQNKLPIYTEENKAIDFTKNSDHYLSSSYKDKSRYATIDFIDQDYHSEYNNVHRIKTF
                     ANYLEDWNGNTLFPLYFVTNSKQDLLKDLEDTKILSELKVDGDITTSDFSTGVIDYSF
                     LKRILMYLAIAVLLCLLSDALTSRKEVFIRKMQGATSWFIYKRLYGKFYLLSILMFVL
                     TQALLYLLVVQNYRPVTHELIQYLLQSALLFVLCLIVVGFIIYVLLSQIQSVVILKKS
                     QFRWISMTLFILKCVFLISIFIPLLTSFKYTSQVVQDGYYLNKYKETYNNYLRLEGVN
                     INMFSLEFQDAMSQLNEKIREYPYIYEDFSTKINRDEDEITYPYIIVNEQYLKEYDLY
                     KDGKPVKTFKPGTLYAPKSVNVDESYCLATECEYDQLDDGYVMLSHSLREDNWHVKNP
                     IIYVVDENYDMSQFGIFIKDKDKTVKKSINDFLKSKGLYQNMNYAYENYDYGKKTMVI
                     NKLIIDSAILLFVYAIVLFLFQYQSIYLYFLENRKMISLNCLLGKNIWQKYRDFIIVN
                     LFVYLVLLLWMVLVVKNISTIGLYLWIFTILIDFMIALIFRTYFERKKVLLSLKGDLE
                     "
     gene            2046..2675
                     /locus_tag="ACZPDN_04575"
     CDS             2046..2675
                     /locus_tag="ACZPDN_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bacteriocin export ABC transporter"
                     /protein_id="HIR1297929.1"
                     /translation="MKVVVVDKIHKQYHDHVIFDDFSMEIEQGSFVAIQGASGSGKST
                     LLNMIGLLDSPDKGNIVIFDKKNVKPFSRQANKLLKAKIGYLFQNFALIDNKSVYYNL
                     YLSIDHFSFLDKKERILKALEDVGLKGFENKKICECSGGEQQRVALARLLIKPCQLIL
                     ADEPTGSLDSANKEVVFKILKKMQSQGKTIVIVSHDEELVERADRIINI"
     gene            complement(3124..4047)
                     /locus_tag="ACZPDN_04580"
     CDS             complement(3124..4047)
                     /locus_tag="ACZPDN_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIR1297930.1"
                     /translation="MNIYKQKLSVTDQVKRLEDKGISFNVFCKERAEDYLTSNNYYYK
                     LTSFRKNFRKHPDGVNKGKYVNLDFAYLVDLAIIDNYLRGIILEIALDIEHYSKLKLL
                     HRLENDEAEDGYSIVNDFMISLGQNRQVLEKRILEKVNSSYVGDIIQHYNGNYPAWTL
                     IEIVSFGDYLRFYKFCADRWNDKDLLNDFYLMKDVKELRNAAAHNNCILNDVTIKESK
                     HQTNHAVKISLKSIKRQRNDKYLAKEKIRQIVTLLYASIHMITSDGVKRKINNQLKFL
                     KNRIYRDYDYSFNQKLKATFDYLFDVIDIYY"
     gene            complement(4079..4423)
                     /locus_tag="ACZPDN_04585"
     CDS             complement(4079..4423)
                     /locus_tag="ACZPDN_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297931.1"
                     /translation="MNREIKFASTVEKYMRMNGLTMKELGEKVGRGESTVSMWIANKS
                     TPPMGIVQKLADLFGVTTDIMIYGDDSSDIALTDHERKHLEIYRLLDDKGQHTVDTVT
                     QMEYERVKKDNK"
     gene            4515..4700
                     /locus_tag="ACZPDN_04590"
     CDS             4515..4700
                     /locus_tag="ACZPDN_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1297932.1"
                     /translation="MQERISIEEAIREAGLKRKYVANKLGVSDTYINEYLKKPGSISV
                     KNASIICKLININYIYV"
     gene            4847..5896
                     /locus_tag="ACZPDN_04595"
     CDS             4847..5896
                     /locus_tag="ACZPDN_04595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1297933.1"
                     /translation="MGAYDNCIILIPALQPDERLIQMIHGLHERGFHKIAVTDDGSSD
                     DRQKYFQEVEIMGVKVIHHSKKMGKGVALRSALRLADEKKGESDFYITADCDGQYSPE
                     DIEKVAKELLENPDCFVLGVRTYERKKGKKVRFWLNSCQKLFFRITNHGKECPDPRSG
                     LRGFPASLKKLALCTEGKSYDYELNFLDAAIQKTPVVVVPISSETHNINETSHFRPVV
                     DLLGMYLKFWKYLFTSNVATVFDLVFFIIFESLFSKLGTISIFVATICARAISGSVGY
                     ILNRYISFQSKLNPGREMVRYSIVFVCQMAASGILVSLLSVIPIPTVIVKAIIDTILF
                     FVVYNIQKNWVFTEK"
     gene            complement(5929..6792)
                     /locus_tag="ACZPDN_04600"
     CDS             complement(5929..6792)
                     /locus_tag="ACZPDN_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1297934.1"
                     /translation="MKTNTIKSFLTITLAMFIISVAIYFFLIPSGVITGSVTGLAMVL
                     AKITSLSISTLTFVINALLLILGIILIGKEFGAKTIYASLLLPLFLAVFERIYPNNIS
                     LTQNAVFDMATYILILAFGQTILFRVNASSGGTDIIAKIVNKYTHMDLGKACTLVGLL
                     ICTTSLIVYDIGALVVGALATLANGQAVDYFIDGFNKKKKICILSKEYEVIQDYIMNT
                     LKRGVTLYSAKGGYDKTEHTELVTIMATNEYKKLLYYLQESDIEAFVTVSTVNEVIGT
                     WNTKKRVEKLR"
     gene            complement(6845..7699)
                     /locus_tag="ACZPDN_04605"
     CDS             complement(6845..7699)
                     /locus_tag="ACZPDN_04605"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR1297935.1"
                     /translation="MTNIAIIGLGLMGASFAKKLTHLNYNVYGIDVSKQTIDKAKELK
                     IIVDGSTNPLDVISKCDTFIFCLYPTKILPWIEVYQNDIPKHSLLMDISGVKTNIVKP
                     IQDVLREDLELLSIHPMCGRESRGIEFSDETIFKGSNFILVPTQKNKNETVEAVKELA
                     NKMEFGRICTLSIEEHDKMIGFLSQLTHVIAVSLMNTHDNAHLVEYTGDSFRDLTRIA
                     KINEDLWTELFIMNKDILLDEINQFIDSISHFRDSLEQEDTQEMKRLFIQSTKRRDKF
                     NKTDAKKR"
     gene            complement(7696..8958)
                     /gene="aroA"
                     /locus_tag="ACZPDN_04610"
     CDS             complement(7696..8958)
                     /gene="aroA"
                     /locus_tag="ACZPDN_04610"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1297936.1"
                     /translation="MKVKVTPSKVHGNVKIPASKSMAHRALICASLSDGTSIVSNVTN
                     SKDIEATVGCMKALGAKIKQIDETTYEVTGTNLFKQEGNITCSANESGSTLRFLIPLA
                     ACTNAKVKFLGQGRLLQRPMDVYENEFKEQNIEFNQSDKEIIVHGNLQAKDYVVQGDI
                     SSQFITGFMFVLPLLNQDSTLTITKPYESKSYVNLTIQMLAKFGIEIIETSSNSYLIK
                     GNQHYKAQDVSVEGDFSQLAFFAVLGTLNNTLSCSNMDMSSIQGDKAILDCIPSFTSN
                     KDTITFAKKQPAPQTIDLSNCPDLGPILCVLASFTNGETNIVNAARLRMKESDRILAM
                     ETELKKWGVDITSTFDSIQIHGKEHYKSDSTVEIFGHNDHRIVMAMTVFGLCAESECI
                     IDDAQAITKSYPTFFEDILSLGGKVEIL"
     gene            complement(8955..10064)
                     /gene="aroC"
                     /locus_tag="ACZPDN_04615"
     CDS             complement(8955..10064)
                     /gene="aroC"
                     /locus_tag="ACZPDN_04615"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1297937.1"
                     /translation="MKNNFGTNISWTLFGESHGPAIGITLDGLPAGFKVDMEQIKSDM
                     DKRKATGKISTQRHEADEVHIISGMYNGYTTGTALTIIIENQNTKSKDYSAFHGRLRP
                     GHADFTAFEKYNGYQDYRGGGHFSGRLTACIVAAGSICRQILKSQGIVIGSHIESLYT
                     LSDIPFSKDYTVLEKQIQEVNDKNFAVLDNQVKNQMIQEIEKAAAEGDSLGGVLETAV
                     IGLPAGLGEPFFDSVESILSHLIFSVPAVKGVSFGEGFGFAKLKGSQANDAFRFNDKI
                     ETKTNHNAGINGGITNGMPILIHTCIKPTPSIFKTQESVDYLSKENVDLKIQGRHDPC
                     ILHRARIVVDSVVAFGLLDLYMSRLAIKPLQGDFE"
     gene            complement(10054..11067)
                     /gene="aroB"
                     /locus_tag="ACZPDN_04620"
     CDS             complement(10054..11067)
                     /gene="aroB"
                     /locus_tag="ACZPDN_04620"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR1297938.1"
                     /translation="MKLNVDLENHSYPIYIERNAIQKVHEYFPVSRKIAIITDTGVPE
                     KWVNIVKEQCPDSFICTIPQGEPSKCFDQYKKLLEEMIQHNMSRKDAIIAVGGGVVGD
                     LAGFVAASYMRGIDFYNIPTTVLSQVDSSVGGKVAIDMGSYKNIVGAFWQPKTVIIDP
                     NVLSTLSFRQQHNGLCEALKMGLILDDHLVSLFEQETLDIDAIITRSIELKRDVVQQD
                     ERESNLRKILNFGHTIGHAIESAYGLNTYLHGECVAMGMLFFIEDEALKQRVLNIYKK
                     LDLPQVPDYDTATLLEYVTHDKKSSHNTVSTILVKQSGSYIIKELSFEDIQNVLERGP
                     YEK"
     gene            11150..11311
                     /locus_tag="ACZPDN_04625"
     CDS             11150..11311
                     /locus_tag="ACZPDN_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014069.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1297939.1"
                     /translation="MEYLVKAQDYLKLKIKDTYRVTNVGKEIAKKEGLNVEDLMIACL
                     LHDVSDFEC"
     gene            complement(11338..11865)
                     /locus_tag="ACZPDN_04630"
     CDS             complement(11338..11865)
                     /locus_tag="ACZPDN_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297940.1"
                     /translation="MKLAILFPGIGYHTDKPLLYYSKKILKNMDYEIKEIHFENLDFD
                     LNKSKDEAYKQAKDQIHKFDLKAYDSILFISKSIGTYCAAKLAHEYKLTANIYFTPLD
                     FTLEYLQQKDLVYSGTQDQWANFDKIEHYCIHHLIEFHSIVNGNHSLETGNIQTDIEN
                     LKQIMYRVETFIHSL"
     gene            complement(11881..12324)
                     /locus_tag="ACZPDN_04635"
     CDS             complement(11881..12324)
                     /locus_tag="ACZPDN_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019191.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HIR1297941.1"
                     /translation="MKKGLKVFLTAAIVLSAVACSTTSDEETKNKDTKEKVVETKKDT
                     SVPKEYRNALHKAENYSKMMHMSKARIYDQLTSENGEKFTAEQAQYAIDNIKGDYLEA
                     ALKSAKNYQETMNMSKDAIYDQLTSEYGEKFTAEEAQYAIDNLDE"
     gene            12824..13051
                     /locus_tag="ACZPDN_04640"
                     /pseudo
     CDS             12824..13051
                     /locus_tag="ACZPDN_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014368.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
BASE COUNT         4289 a         2018 c         2183 g         4612 t
ORIGIN      
        1 ggacaatcgc aagaaaaaat aatgaaaaag gaggacagcc ggaatcatgc ccccggaaac
       61 cgggcggcag attgttccgg cttttgtatg cctatgaaac aatataatga tgtgataaat
      121 ctgagttaca agtttgctca agaaaacaat atggaagttg ttccaataga tttacaattg
      181 agtgaagata agcaagaaag aacaaaacaa tttaatcaag tatacagttt tttgcaagaa
      241 cattcgtatc cttgttttgt tggcatagtt tctaaaaata taaaaacgga atatgtatat
      301 cttccaaaac aaaataaact accaatttat acagaagaaa ataaagcgat tgattttact
      361 aaaaatagcg atcattattt gtcttcttct tataaggata aaagtaggta tgcaacgatt
      421 gattttattg atcaagatta tcatagtgaa tacaataatg tgcatagaat aaagactttt
      481 gcgaattatc tagaagattg gaatggaaat acactatttc cgttgtattt tgttacaaat
      541 tctaagcagg atttgttgaa ggatttagag gatacgaaaa tattatctga attgaaagtg
      601 gatggtgata tcacaacatc cgatttttct acaggagtga ttgactattc gtttttaaag
      661 agaattttga tgtatttagc aattgcggtt ttattatgtc ttttatccga tgcattgaca
      721 tctagaaaag aagtttttat tcgtaagatg caaggtgcta cttcatggtt tatatataag
      781 aggctatatg gaaaatttta tttgttaagc atattgatgt ttgtgttaac acaagcttta
      841 ttatatcttc ttgtagttca aaattataga cctgttactc atgaactcat tcaatattta
      901 ctgcagtctg ctcttctttt tgtactttgt ctgattgtag ttggatttat aatatatgtc
      961 ttattaagtc aaattcaatc ggttgttata ttgaaaaaat ctcagtttag atggatttca
     1021 atgactctgt ttatattgaa atgtgtcttt ttgatttcaa tatttatacc attattgact
     1081 tcgttcaagt atacatctca agtggttcag gatgggtatt acttaaataa atacaaagaa
     1141 acctataata attatcttcg tttagagggt gttaatataa atatgttttc tttggagttc
     1201 caagatgcga tgagccagtt gaatgaaaaa attcgagaat atccatatat atatgaagat
     1261 ttctcaacca aaataaatcg tgatgaggat gaaataacat atccttatat cattgtaaat
     1321 gagcagtatt taaaggaata tgatttatat aaggatggta agccagttaa aacatttaaa
     1381 ccgggtactc tttatgctcc aaaatcagtg aatgtagatg aaagttattg tttggctacg
     1441 gagtgtgagt atgatcaact tgatgatggt tatgtcatgc tttcgcatag tttaagagag
     1501 gataattggc atgtaaaaaa tccaattatt tatgttgtgg atgaaaacta tgatatgagt
     1561 caatttggta tttttattaa ggataaagat aaaaccgtaa agaagtccat taatgatttc
     1621 ttgaaatcta agggattgta tcaaaatatg aattatgcgt atgaaaatta tgattatgga
     1681 aaaaagacca tggtaatcaa taaattgatt atagattcag ctatattact ttttgtctat
     1741 gcgattgttt tattcttgtt ccagtatcaa tctatatatt tatatttttt agaaaatcga
     1801 aagatgattt ctttaaattg tttgttggga aaaaacatct ggcaaaaata tcgtgatttt
     1861 attattgtga atctgtttgt ttatcttgtc ttgctgttgt ggatggttct agttgtaaaa
     1921 aatatttcga caataggctt gtatttgtgg atattcacaa ttctaatcga ttttatgata
     1981 gctctaattt ttagaacgta ttttgaaaga aagaaagtgt tattatcctt gaaaggagat
     2041 ttggaatgaa ggtagtagtt gttgataaga ttcataaaca atatcatgat catgtgattt
     2101 ttgatgattt ctcaatggaa atagaacaag gaagttttgt cgcaatacaa ggtgcttcag
     2161 gatcagggaa atctacattg ttaaatatga ttggtctttt ggattcccca gataaaggaa
     2221 acattgtgat ttttgataag aaaaatgtga aaccattttc tagacaagct aataaattgt
     2281 taaaggcaaa aataggatat ttattccaga attttgcgtt gattgataat aagagcgtgt
     2341 attacaactt gtatttgtct attgatcact tttcatttct agataagaag gaaagaatat
     2401 taaaagcact tgaagatgtg ggtttaaaag gtttcgaaaa taaaaagata tgtgaatgct
     2461 ctggtggaga gcaacaaaga gttgccttag ctcgtctttt aatcaaacca tgtcaattga
     2521 ttttggcaga tgaacctaca ggaagtttgg attcagcaaa caaagaagtt gtgtttaaaa
     2581 tcctaaagaa aatgcagtca cagggaaaaa caattgtgat agtcagtcat gatgaagagc
     2641 ttgtagaaag agcagatcgt ataataaata tctaaaatac actttaagca cgcgtataag
     2701 acgcaaaaat aactgaatat gctttaaata aagaaacatt cagttctcta aacgttagta
     2761 ttttacttac taaaagaaag caaaagtctc tatataaagg attttttctt ttgcctaaat
     2821 actatgaatt cgtatgtgta ggtcacaaat aggtcacaca actttttaaa ctaaaaacct
     2881 cgaagaatat ctaaaatggt agtcaccttt ttatgtgaga gtattggtag tactcttaat
     2941 cgtcttggat gttcgaagta tccaggatat ttttttataa aagaaaaagc cttaatcaaa
     3001 acttgatctc tgataacgag agttacgatt aaggagtagt taataaaaat aggaagcagt
     3061 gtcgtttcca agactgcttc ctcaaaacta atgtttttcc ttgcaaacgt agtttaacac
     3121 agattagtaa tagatgtcaa ttacatcaaa taagtaatca aatgttgcct taagtttctg
     3181 attgaagcta tagtcgtaat ctcggtatat tctattcttt aaaaatttca attgattatt
     3241 gattttgcgc ttaaccccat cgcttgtaat catgtgaatt gatgcgtaaa gtagtgtaac
     3301 gatttgtctg attttttctt tagcaagata tttatcattt ctttgacgct ttattgattt
     3361 tagactgatt tttactgcat gattagtttg gtgcttcgat tctttgattg taacatcgtt
     3421 taatatacaa ttattatgag cagctgcatt tcttaattct tttacatctt tcataagata
     3481 aaagtcattc agtaagtctt tatcgttcca tctatctgca caaaatttat agaatcttaa
     3541 ataatcgcca aaagatacaa tttctatcaa tgtccatgct ggataatttc cattgtaatg
     3601 ttgaatgata tcaccaacat aagaactatt gactttttca agtattcttt tctctaaaac
     3661 ttgtctattt tggcctagac ttatcataaa atcatttact attgaataac catcttcagc
     3721 ttcgtcattt tccaaacgat gtaataattt aagtttggaa taatgctcta tatctagagc
     3781 aatttcaagg ataatacctc gcaaataatt atcgataata gctagatcaa ctagatatgc
     3841 aaaatcaaga tttacatatt tacctttgtt tactccatca ggatgttttc taaagttttt
     3901 tcggaaagat gttaacttat aataataatt gttgcttgtc aaataatctt cagcacgttc
     3961 cttgcaaaaa acgttaaagg aaattccttt atcttctaaa cgtttcacct gatctgtaac
     4021 gcttaatttt tgtttgtata tattcaccct atccctcctt tatataatca tacctaaatt
     4081 acttattatc cttcttaact ctttcgtatt ccatttgtgt gactgtatcc actgtgtgct
     4141 ggcctttgtc atctagtaat ctgtaaattt caaggtgttt gcgttcgtga tcagttaatg
     4201 caatatcgga tgagtcatca ccatatatca ttatatctgt agtaacgccg aatagatcag
     4261 ctagtttttg tactataccc attggaggtg tagatttgtt agcaatccac attgatacag
     4321 tactttcacc acgacctact ttttctccta gctctttcat tgttaaacca ttcattcgca
     4381 tgtacttctc aaccgtgcta gcaaacttaa tttctctatt cattttttca cctctttcta
     4441 tttaattata aaatatattc aataatattc aaaaaaattg aaatacatta tgaatgtgca
     4501 aggaggtgat atagatgcaa gaaagaatca gtattgaaga agcaattagg gaagcaggtc
     4561 ttaaaaggaa atatgtagcc aataaattag gtgtttcaga tacatatatt aatgaatatt
     4621 tgaaaaagcc tggctctatc tcggttaaaa atgcatcaat catttgtaaa ttaataaaca
     4681 ttaattatat ttacgtataa gagatttcaa aatcatggat ttattccatg gtcttttttg
     4741 tatattcaac cgaattgaat atttttcttt cattgtatat atctttcgat tcaaaagcca
     4801 tatatgataa aggtatagta tacatatttt gtttagggag aagaagatgg gagcatatga
     4861 taattgtatt atcctgattc cagcattaca gccagatgag cgcttgattc aaatgattca
     4921 tggattacat gaacgtggtt ttcataaaat tgctgttacg gatgatggaa gtagtgatga
     4981 tcgacagaaa tactttcaag aagtagaaat aatgggcgtt aaagtgattc atcattctaa
     5041 aaaaatggga aaaggggtag ctttaagaag tgcattacga ctggcggatg aaaaaaaagg
     5101 agaatctgat ttctatataa cggccgattg tgatggtcaa tactcgccgg aagatattga
     5161 aaaggtcgca aaagaattat tagaaaatcc agattgtttt gtgctcggag tacgtacata
     5221 cgaaagaaaa aaaggtaaga aagtaagatt ttggttgaat tcttgccaga aattattttt
     5281 ccgtattacc aatcatggaa aagaatgtcc ggatcctaga agtggacttc gtggttttcc
     5341 agcttcatta aaaaaactag ctctttgtac agagggaaag tcttatgatt atgaattaaa
     5401 ctttttagat gcagctatac aaaaaacacc agttgtggtt gtacctataa gttcagaaac
     5461 acataacatc aatgaaacaa gtcattttcg tccggttgta gatttactag gaatgtattt
     5521 aaaattctgg aaatatctat ttacatcaaa tgttgcgact gtatttgatt tagttttctt
     5581 tataatattt gaaagtttgt tttctaaact cggtacgatt tcaatttttg tggcaactat
     5641 atgcgcgaga gctattagtg gtagtgtggg atatatatta aatcgatata ttagctttca
     5701 gagtaaatta aatccaggaa gagaaatggt tcgctattcc attgtgtttg tatgccagat
     5761 ggctgccagt ggtattttgg tatccttact aagtgttatt ccaattccta ctgttattgt
     5821 aaaagcaatc attgatacaa ttttattttt tgtcgtctac aatattcaaa aaaattgggt
     5881 atttaccgag aaataaaaaa gacatcctgc tttgggatgt ctttaaattt atctaagctt
     5941 ttcaactctt ttcttagtgt tccaagtacc gatgacttca ttaactgtag atacagtgac
     6001 gaatgcttca atatcacttt cttgaagata atatagtaat tttttatatt catttgtagc
     6061 catgattgtg acaagctctg tatgttctgt cttatcataa ccaccttttg ccgaatataa
     6121 agtaacacca cgctttaatg tgttcataat ataatcttga atcacttcat attctttgga
     6181 taagatacaa atcttctttt tcttattaaa accatcaata aaataatcta cagcctgacc
     6241 attggctagg gttgccaatg caccaaccac taaagcacca atatcatata caattagact
     6301 tgttgtacaa atgagtagac cgactaatgt acaagctttg cctagatcca tatgtgtata
     6361 tttattgacg attttcgcaa taatatctgt tccaccactt gatgcattta cacgaaatag
     6421 tatggtttgt ccaaaagcta aaattaatat gtatgtagcc atgtcaaaaa cagcattttg
     6481 tgttaaagaa atattgtttg gatagattct ttcaaataca gctagaaata aaggtaataa
     6541 taaagaggca taaatcgttt ttgctccaaa ttccttacca attagaatga tacctagaat
     6601 caacaataag gcgttgatca caaaggtaag tgtagaaata gataggcttg tgattttagc
     6661 taaaaccata gctaaaccag taacacttcc ggtaataaca ccactaggaa ttaagaagaa
     6721 atatatggcg acactgatga taaacatggc aagtgtaata gttaaaaaac tttttattgt
     6781 attcgttttc atttgtaaaa cctccgcttt ttattatgcc aaaaaaagta taaaaaaaga
     6841 agagttatct cttcttcgca tcggttttat taaatttatc tctacgcttt gtagattgaa
     6901 tgaatagacg cttcatttct tgtgtatctt cttgttctaa agaatctcta aagtgagaga
     6961 ttgaatcgat aaactggttg atttcatcta ataagatatc tttattcata atgaagagtt
     7021 ccgtccatag atcttcattg attttcgcaa tacgcgttaa atctctaaaa ctatctcctg
     7081 tgtattctac aagatgtgca ttatcatgtg tattcattaa agataccgca atgacatggg
     7141 tcaattggga taaaaaacca atcattttat catgctcttc aatagataat gtacaaattc
     7201 tgccaaattc catcttgtta gctaattcct ttacggcttc aactgtttca tttttattct
     7261 tttgagtagg aacaagaata aaatttgaac ccttaaaaat cgtttcatca ctaaattcaa
     7321 taccacgaga ttctctacca cacatggggt gaatacttaa taattcaaga tcttcgcgta
     7381 atacatcttg aataggtttg acaatgtttg tcttaacacc actgatatcc atcaatagtg
     7441 aatgtttagg aatatcattt tgatagactt cgatccaagg aagaatttta gttggatata
     7501 gacagaagat aaatgtatca cactttgaaa tgacatctaa aggatttgtg ctaccatcta
     7561 cgatgatttt taattctttg gctttatcaa tcgtttgttt agaaacatca ataccataca
     7621 cgttgtagtt taagtgggtt aatttcttcg caaaacttgc acccattaat ccaagtccaa
     7681 taatcgctat gtttgtcata gaatttcgac ctttccacct agacttaaaa tatcttcaaa
     7741 gaaagtagga taactctttg tgatggcttg ggcatcatca ataatgcatt cagattctgc
     7801 gcataaccca aaaaccgtca ttgccataac tatacggtga tcattatgtc caaagatttc
     7861 tactgtacta tctgatttat agtgttcttt accgtgaatt tgaatggaat caaatgtaga
     7921 agtaatatcc acaccccatt ttttaagttc ggtttccata gcaagaatac gatcactttc
     7981 tttcatacgt aaacgagctg cattcacaat atttgtttca ccatttgtga aactggctaa
     8041 gacacataag attggtccta aatctggaca attagataag tcgatagttt gtggtgcagg
     8101 ttgttttttt gcgaatgtga ttgtatcctt gttacttgta aaactaggaa tacaatctag
     8161 tattgcctta tctccttgaa tactagacat gtccatgttg gagcacgaaa gagtgttgtt
     8221 taaagtgcct aataccgcaa agaaggctag ttgagaaaag tctccttcaa cagaaacgtc
     8281 ttgtgcttta tagtgttggt ttcctttgat taaataggaa tttgaacttg tttctataat
     8341 ttcaatacca aatttcgcta acatttgaat cgtaagattt acatagcttt ttgattcata
     8401 aggcttagta attgttagtg tactatcttg atttaataat ggaaggacaa acatgaatcc
     8461 tgtaataaat tggctggaaa tatctccttg tacaacatag tcttttgctt gtagatttcc
     8521 atgtacaatg atttctttat ccgattgatt aaattcaatg ttttgttctt taaattcatt
     8581 ttcatataca tccatgggtc tttgaagcaa tcttccttga cctaaaaatt taactttggc
     8641 atttgtacat gcagcaagtg gaatcaagaa acgtaaagta gaaccggatt cattcgcact
     8701 acatgtaata tttccttctt gtttgaaaag attagtacct gttacttcat atgtagtttc
     8761 atcgatttgt ttaattttag cacccaaagc cttcatgcag ccaacagttg cttcaatatc
     8821 ctttgagttt gtgacattag aaacaatact tgtaccatca cttaatgatg cgcaaattaa
     8881 agctctatgc gccatagatt tggatgcagg tatttttaca tttccatgta ctttacttgg
     8941 tgttactttt actttcattc aaaatccccc tgaagtggct taatggcaag cctagacata
     9001 tataaatcta ataaaccaaa cgcaactaca ctatccacaa caattcttgc tcggtgcaag
     9061 atgcatgggt cgtgtcttcc ttgaatttta agatccacgt tttcttttga aagataatcc
     9121 acagattctt gtgttttaaa aatcgatggc gttggcttaa tgcaagtatg gatcaaaatc
     9181 ggcataccat ttgtgatacc accattgatt ccggcattgt gatttgtttt agtttcaatt
     9241 ttatcattaa aacggaaggc atcattggct tgtgagcctt ttaatttcgc aaagccaaat
     9301 ccttcgccaa aggaaacacc tttgacggct ggtacagaga aaattaagtg ggataaaatg
     9361 ctttctacag aatcaaagaa aggttctcct agtcctgcag gaaggcctat aacggcggtt
     9421 tctaaaacac cacctaatga atcaccttca gcagctgctt tttcgatttc ttgaatcatt
     9481 tgatttttga cttgattatc taaaaccgca aaatttttat cattgacttc ttgaatttgt
     9541 ttttctagaa cagtgtagtc tttagagaaa ggaatatccg atagagtata taaactttca
     9601 atgtgtgatc caattactat gccctgtgat ttcagaattt gtcgacatat acttccagca
     9661 gctacaatgc atgcggtaag tcttccggaa aagtgtccgc caccacgata gtcttgatat
     9721 ccattgtatt tttcaaaggc agtaaaatca gcatgtccag gacgcaatct tccatgaaaa
     9781 gcagaatagt cttttgattt agtgttttga ttctcaatga taattgtaag tgcagttcct
     9841 gttgtatacc cgttatacat tcctgaaata atatgcactt catccgcttc atgtctttgg
     9901 gttgaaattt tacctgttgc cttacgctta tccatgtccg atttgatttg ttccatatcg
     9961 accttaaagc ctgcaggaag accatctaat gtaataccaa tggcaggacc gtggctttca
    10021 ccaaataatg tccaagatat atttgtaccg aaattatttt tcataaggtc ctctttctaa
    10081 tacgttttgt atgtcttcaa aagaaagttc tttgatgata taagaaccag attgttttac
    10141 tagaattgta gagactgtgt tatgactgct tttcttatca tgtgttacat attctaataa
    10201 tgtggcagta tcatagtctg gtacttgagg caaatctagc ttcttatata tatttaatac
    10261 gcgttgtttt aatgcttcat cttcaataaa gaataacata cccatcgcca cgcattctcc
    10321 atgaagatat gtatttaatc catatgccga ttcaatggcg tgtccaatgg tatgtccaaa
    10381 gtttagaatc tttctaagat tactttctct ttcatcttgt tgaactacat ctctctttaa
    10441 ttcaatggaa cgcgtaataa tcgcatcgat atctaatgtt tcttgttcaa ataaagagac
    10501 taaatgatca tctaaaatca atcccatttt taaagcttca cataatccat tatgttgttg
    10561 gcgaaaagaa agcgtagata atacgtttgg atcgataata acagttttag gttgccagaa
    10621 agcaccgaca atatttttgt aggaacccat atcaatagca actttaccac ctacacttga
    10681 atcaacctgg cttaatacag tcgttgggat gttgtagaaa tcaatgccgc gcatgtagct
    10741 tgcagcaaca aaacctgcaa gatctccgac aaccccaccg cctaccgcaa taatggcatc
    10801 ttttctagac atgttgtgtt gaatcatttc ttctaaaagc tttttatatt gatcaaaaca
    10861 tttggatggt tcaccttgcg gtatggtgca tataaaagaa tccgggcatt gttctttgac
    10921 aatattgacc catttttcag gcacacctgt atctgtgata atcgcaatct ttcttgatac
    10981 aggaaaatat tcatgtactt tttgaattgc gtttctttca atatatattg gatatgaatg
    11041 attttctaaa tcgacattta atttcataat aaaaaactcc tttcgtatat cataccatga
    11101 acataagctt ttttcagaaa ttttctgaaa gaagtagttc aatgaataaa tggagtactt
    11161 agttaaggct caagactatt tgaaattgaa aataaaggac acatatcgtg tcacaaatgt
    11221 tggaaaggaa atcgcaaaaa aagaaggatt gaatgtagaa gatttaatga ttgcgtgttt
    11281 gttgcatgat gtgagtgatt ttgagtgtta aaagatcaat taaaaatatc cacagcctta
    11341 taagctgtgg ataaatgttt ctacacgata catgatttgt ttcaaattct caatatctgt
    11401 ttgaatattt cccgtttcta aagaatgatt cccattaaca atactatgaa attcaataag
    11461 atgatgaata caataatgtt caatcttatc gaagttagcc cattggtctt gagttccaga
    11521 atacactaaa tctttttgtt gaagatattc caaagtaaaa tctaatggcg tgaaatagat
    11581 attggcagtt aacttatatt catgggcaag tttagctgcg caataagtac ctatactttt
    11641 tgagataaag agaatggaat cataagcttt taaatcgaat ttatgaattt ggtctttggc
    11701 ttgtttataa gcctcatctt tagatttatt taaatcaaag tctaggtttt caaaatgaat
    11761 ttctttgatt tcataatcca tattctttaa tatcttcttt gaatagtata gtaatggttt
    11821 atccgtgtga tatccaattc caggaaataa tatagcgagt ttcatgcttt tagtatagga
    11881 ttattcatct aaattatcaa tcgcatattg tgcttcttca gctgtgaatt tttctccata
    11941 ttccgaagtt aattgatcat agatggcatc ttttgacatg ttcatagttt cttgatagtt
    12001 ctttgcggac tttaaagctg cttctaaata gtctcccttg atgttatcaa tggcatattg
    12061 tgcttgctcg gcagtaaatt tttctccgtt ttcagaagtt aattgatcat agattcgagc
    12121 ttttgacata tgcatcattt tactgtaatt ttcagcttta tgcagtgcat tacgatactc
    12181 ttttggaaca ctagtatctt tctttgtttc tacgaccttt tcttttgtgt ctttattctt
    12241 tgtttcttca tcactcgttg ttgaacatgc gacagccgat aagacaatag cggctgttaa
    12301 aaatactttt aaaccctttt tcatatatac tccttatttc tttttcttta gtataagaaa
    12361 aatataaaaa ggtttggtat gccatgagca tacatttaaa ttagggaatc aatccacata
    12421 aaaaaccata cgtctaaaca tacgaatata aagagaatga tggaataaag tataggccaa
    12481 gggactttag acatgtgttt atggaataaa ttcttgttgt agtaaaggaa taaaccaaaa
    12541 tatataaaca aaaaacaact tagtttatta ataaaactat ttttaatggt ttcccctggc
    12601 tccatatcga ccacaattat aagtgtaaat acaatccaag ctaaactgat catcttagat
    12661 ttgaatgtgt cattattaag tgggggaata acaactagtt tttgttgaat ggctagattc
    12721 atctctttca cagatttgta gcggttctta taatcgagtt gtgttgcctt taaaataatt
    12781 tgttttaaat caaaggaaat actgttttga tgttaaatta aatgttgaaa aagtggctaa
    12841 aaagatggga cttgatgtac aagaaattca tcactgtgca gtgagtgaag gaaagtctgc
    12901 tgctggaaat tatgatatcg tattgtgttc aaaaaacttc gttcctatgt ttgaagactc
    12961 aaagaaacgt ggaactaaag ttgttggact taagaatgta atgtctgcaa aagaaatcga
    13021 agaaggctta aaagcagcac ttagtgaata ataaaaaatg aaaagctgcg tttatgcagc
    13081 ttttcttata cccaaaatta cc
//
LOCUS       DBMDWL010000045        12687 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1674_length_12687_cov_3.209836, whole genome shotgun sequence.
ACCESSION   DBMDWL010000045 DBMDWL010000000
VERSION     DBMDWL010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12687)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1674_length_12687_cov_3.209836"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2293
                     /locus_tag="ACZPDN_04645"
     CDS             <1..2293
                     /locus_tag="ACZPDN_04645"
                     /inference="COORDINATES: protein motif:HMM:NF052597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FILTED domain-containing surface protein"
                     /protein_id="HIR1297942.1"
                     /translation="GYDVTITEGSKYTTDDFTFNGTAEAKGTEAGTYPMNLAADQFTN
                     TNDNYTQVTFIVNDGTLTINPKSITPDGPDTPKEEKTGITVTDPINSTYDGNEHINGL
                     TVTDSKLNTTLAEGTDYTLTYSGDLINVGTVTITIKGIGNYTGEFTKTYQILPREYTV
                     TTNTDSKVYDGNPLTAGGTVNNLVDGETVGFTMTGSQTNVGTSNNTYELNWTGSAKES
                     NYKHGKDSIGTLTVTKQSIAPDPEHPETYKEVTITSPSDEVYDGKEHKWIPTVTDKEE
                     NELVAKTDYEVTYSTTDFTNVTGTITVTITGIGNYTGKATRTYSITPKTYTVTTESDS
                     KVYDGTALTAGGKVSGIVKGETVDFTITGSQTSVGTSDNTYELDWTGSAKESNYKHGK
                     DSIGTLTVKAKSIVPDGPDTPDEKKTGITVSDPSDSKYDGKEHREVLTVQDTKKNDEL
                     VAEKDYSVTYSDDLVNAGTVTITIEGIGNYTGSFTKTYEITKRSVTLTSATASKTYDG
                     QALTSTSITVSGDGFVEGEGATYNVTGTQTEVGNSANSFEYKLNENTLASNYDITKVV
                     GTLTVTRDTTPVTPAPTPTTPSVPPVVQRVVPTPTPVPEEKVEEEQTPKAEPKEEEKV
                     EEDYTPKATQEYYWALINLICAIVTVLFGLLLLISKRHKDEDDEEEDDETKQQATTNE
                     DEEQEQEKKRGLFTRVLAVLIAIVSVVFFLVTEDMSLAWTWTDQWTIWMVVIGLVQIV
                     VFFVGRKWKNVDNDDDDEEAQQA"
     gene            complement(2328..3725)
                     /locus_tag="ACZPDN_04650"
     CDS             complement(2328..3725)
                     /locus_tag="ACZPDN_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1297943.1"
                     /translation="MKYGENNNIEVKKEVPAEHKKYLKSVVAFANGFGGKIIFGIEEK
                     KDSFEICGLSGDVFKIKDSITNSIFSNIEPHIIPSIDLDTIEDKTILIVNVPSGWDRP
                     YHLKAEGEFDGVYVRVSGTTRKADRNMIKELMFEGSNRYFDKMICPELEISENSIQNL
                     CDTLYHKALENSKSQFEKDAVTPITKQDLLSWGILTQSNNHIVPTNAYALLTGHPALP
                     NRIQCALFKGTDTTEVIDMKDYSGPIYEQIDFAYKFVLRNIRLHASFESLERKETYEI
                     PVYAIRELIVNSIAHRSYIDRNSIKIAIFDNRLEVTSPGKLVMGQTIEKMKQGNSNIR
                     NEATAYALRYLKYIENWGRGIPRIIKSIEECGLKPPVFIGGETELKVIIYRNNVFYKP
                     NKKIGFKVNEPNGPNEPDIIQLIKINPKITQIELANQLNLSRSTIKRILNRLQQQEIV
                     IRKGSNRNGYWAVKE"
     gene            complement(3801..4889)
                     /locus_tag="ACZPDN_04655"
     CDS             complement(3801..4889)
                     /locus_tag="ACZPDN_04655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1297944.1"
                     /translation="MPTIIENRRNLHQIPEIELTTQKTAQYIENVLDLYDCTIDHPIP
                     NSVTAFFDNKKEKTIVFRSDMDALPMEENTDLEFKSTNGCMHACGHDGHMAMVLEIAK
                     YTKESNYNVLLLFQPGEEKPGGAKPIIESGFFKQFNIAAIYGTHIYPELEKGKVYTRP
                     NEMMAGGCEFEILVKGKASHAAQPQNGINALTACVEILQKSLEQEEKAFDNNTFHLLH
                     FGTFHSGQAFNIIADQAHVRGSIRYYDPKVFDQIVNIIEQNTNNAIKKYKVQVNFKVN
                     HHYPPVLNNEELFHTLYNNNLVSKLDSPVLISEDFGYYRNVAPSIFFFLGTGDTIPLH
                     SNKFTFDEDILKKGVELYRKLLTTKIPF"
     gene            5028..6641
                     /locus_tag="ACZPDN_04660"
     CDS             5028..6641
                     /locus_tag="ACZPDN_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1297945.1"
                     /translation="MTTTNILSLLGGLALFLYGMQMMGNGLEAAAGDRLKQILEKLTS
                     NRFLGVIVGALVTAVIQSSSATTVMVVGFVNAQMMTLQQAVWIIMGANVGTTITGQLI
                     ALDVSAIAPLVAFMGVALIMFTKSAKATDIGQIIAGLGILFIGMNMMSTAMMPLRESK
                     EFVELVTHFSNPLVGILVGAGFTALIQSSSASVGILQALAASGVIGLDGAVYVLFGQN
                     IGTCITAVLASIGTSREAKQATAIHLSFNIIGTVIFTLVCMFTPLVSLVIHWTPYNAT
                     AQIANMHTLFNICTTLLLLPFGKYLAEFAQHILPVENKETEDDRLLFLQPLPKSKKMI
                     GGAAISVKQINDETMHMLELAYENVNQAFGQLLQFSDKIDEQIYKREDTVNYLDKVIS
                     EYISTALATPNLNVNISQALSSYYLMLVDIERISDYAVNMNHQATKRLQGDMTISEIE
                     IVEHMKKLCVDMKNDLSDVEEAERKDDVIDSLVSSWREAQIQALKQQKCSSEVSMMLS
                     RIFTDFERINDHALNVSQEFDKINIEIPE"
     gene            6689..7438
                     /locus_tag="ACZPDN_04665"
     CDS             6689..7438
                     /locus_tag="ACZPDN_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1297946.1"
                     /translation="MNIEKLLNTIIEAGKMLVESGAEVNRVEETMVRMCRCFEGIEYA
                     DSYVTLTGIMFSLTYENKTITRICRVHTGEVDLNRIDQINTLSRSICTNPISVDELAN
                     ELEKIKKISRYTFKETMFFGAVGAAGFGIFFNGTLLEIVMSFFIGILIRCISCFLSKH
                     KLNSFLNNAISAGSAALSAQLIHQVLPQTNVDIMVISSFMLLVPGLAITNAIRDTIAG
                     DYVSGVARAADAFLCAVAIAVGAGFVMYFWI"
     gene            7451..7906
                     /locus_tag="ACZPDN_04670"
     CDS             7451..7906
                     /locus_tag="ACZPDN_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1297947.1"
                     /translation="MVYVILKSGISSFLASLGFGFLFNIKGRNLWIAGVVGAIGGACY
                     KVSLYLGCSEILSNFYGALGLALASEILARKCKTPVTTFLVCALIPLVPGGGMYRTML
                     AAIRGDAQQTLNMGLQTLSIAGVLALGILLVSTIMRVYYQEKRKYLSKK"
     gene            7964..8040
                     /locus_tag="ACZPDN_04675"
     tRNA            7964..8040
                     /locus_tag="ACZPDN_04675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7998..8000,aa:Ile,seq:gat)
     gene            8066..8141
                     /locus_tag="ACZPDN_04680"
     tRNA            8066..8141
                     /locus_tag="ACZPDN_04680"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8099..8101,aa:Ala,seq:tgc)
     gene            8241..9389
                     /locus_tag="ACZPDN_04685"
     CDS             8241..9389
                     /locus_tag="ACZPDN_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HIR1297948.1"
                     /translation="MEKEFYEEYEDMEAGIDQKQALVEESKKVDEIEDFSEAVRFVND
                     LKKRWRKTGFGESLAEEKLREEFDANIEKVYEKQKALAQKVVDVKEALIKEAEKTSLS
                     DDFKKATEKMTSLMDQWKVSGNAGKKTDDELWERFNAARQKFYDRKHANWENRTVQFE
                     NAKKVKEDLIEKAKSLQDSEEWQKTSAKYKELMDAWKAAGNAGREFDDDLWNAFNEAR
                     QKFYAKRNEFYEKLHAEHDEKYAEKQALVKEAKSIAGLQEYTREQTAMMKELSNKWKS
                     IGFCGKDKEDEIWNEFRGVMDEYFAGLKSASEKRRANWRDHMAEIISRKEEQIANQKR
                     QIKRLQDDMTGLVSEATVADLQDQVEDKEDFIKQLEQEIEDIEKKLAE"
     gene            9423..9617
                     /locus_tag="ACZPDN_04690"
     CDS             9423..9617
                     /locus_tag="ACZPDN_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297949.1"
                     /translation="MNFHECMKLFYPETKIILYNRKNEVVLEGQVNDIRKECEKYHNV
                     EVTFVSGRADTTGLKIWLDM"
     gene            complement(9654..11612)
                     /locus_tag="ACZPDN_04695"
     CDS             complement(9654..11612)
                     /locus_tag="ACZPDN_04695"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1297950.1"
                     /translation="MSKNNIKLGIAGLTAAATGAALFTAKKVSDKKKEEKKVTSDYRN
                     TERGKYEKNSKGIYYTNGNYEAFARPEKPANVDNKNAYIVGSGLAALAAACFLVRDGQ
                     MPGKNIHILEAMDIAGGACDGIYDPTRGYVMRGGREMENHFECLWDLFRSIPSLEIEG
                     ASVLDEYYWLNKHDPNYSLCRATINQGKDAHTDGKFDLSTKGAMEIMKLFMTPDEDLY
                     DKTIEDVFDEEVFDSTFWMYWRSMFAFENWHSALEMKLYFQRFIHHISGLPDFSALKF
                     TRYNQYESLILPMQKYLEAHGVDFQFNTEVTNVEFEFVGDKKVAKTIECKVNGTETGI
                     VLTENDLVFVTNGSCTEGTIYGDQDHAPNGDAEVRTSGCWSLWKNIAKQDPSFGHPEK
                     FCSDISKTNWESATVTTLDDKIIPYITNICKRDPRSGKTVTGGIVTCRDSSWLMSWTI
                     NRQGQFKNQDPNKVCVWVYGLFTDVDGDYIKKPMRECTGKEITEEWLYHLGVPTDQIE
                     ELATNSARCVPTMMPYITAFFMPRTKGDRPDVIPDGCVNFAFLGQFADTPRDTVFTTE
                     YSVRTAMEAVYGLLGVDRGVPEVWGSVYDVRELLDSSVKLMDGKSPLEIDLGPLNVFK
                     KPILKTIKGTVIEKLLKDHDIIKSDMKY"
     gene            complement(11704..12267)
                     /locus_tag="ACZPDN_04700"
     CDS             complement(11704..12267)
                     /locus_tag="ACZPDN_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1297951.1"
                     /translation="MSNLTKLALEQSLKRFLLKKPLDKITIKDLTDDIGISRMSFYYH
                     FKDIYDLVEWSCIYDATNALKGNKTYDTWQEGLTSIFEAVLQNKPFILNVYHTTTQDS
                     IERFLFTTVHDLILGVVREKAQGTNITEEQIQFIADFYKYSFVGIMLNWISKGMSEDY
                     HEIVHNMANTLHGSIANSISNFTNEAK"
     gene            complement(12446..>12687)
                     /locus_tag="ACZPDN_04705"
     CDS             complement(12446..>12687)
                     /locus_tag="ACZPDN_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297952.1"
                     /translation="NDKQVKCIFILCQNRLFDDTYMNMNTLTELLEKSDTTTRKIIKS
                     LEPKNLLVKSRNKNQYLYSANLDSLSSQNFTKCFD"
BASE COUNT         4062 a         1984 c         2451 g         4190 t
ORIGIN      
        1 tggatatgat gtaacaatca ctgaaggaag taagtataca acagatgatt tcacattcaa
       61 tggaactgca gaagctaaag gtactgaagc tggtacatat cctatgaatc ttgcagctga
      121 tcaatttacg aacacaaatg acaactatac tcaagtaaca tttatcgtaa atgatggaac
      181 attaacaatc aatcctaagt caattactcc agatggacca gatactccaa aagaagaaaa
      241 gactggtatt acagtaacag atccaattaa ttcaacttat gatggaaatg aacatatcaa
      301 tggattaaca gtaactgatt ctaagttaaa tacaacatta gcggaaggta cagattacac
      361 attaacttac agtggagatc taatcaatgt tggaacagta acgatcacta ttaaaggtat
      421 tggaaactat actggtgaat ttactaagac atatcagatc ctaccaagag aatatacagt
      481 aacaacaaat acggattcta aggtttatga tggaaatcca ctaacagctg gtggaacagt
      541 taataacttg gtagatggtg aaacagtagg atttacaatg actggtagcc aaacaaatgt
      601 tggtacttca aataacactt atgaattgaa ttggacagga agtgcaaaag aatcgaacta
      661 caaacatggt aaggattcaa ttggaacatt gacagtaact aaacagtcaa tcgcaccaga
      721 tcctgaacat cctgaaactt ataaagaagt tacaattacg agtccaagtg atgaagtata
      781 tgatggtaag gaacacaagt ggattcctac agttacagat aaagaagaaa atgaattagt
      841 tgccaagaca gattacgaag tgacttattc aactacagat ttcacaaatg taactggaac
      901 aattacagtg acaattacag gtattggaaa ctacactgga aaagcaactc gaacttattc
      961 aattacacca aagacttata cagtaacaac tgaatctgat tctaaggttt atgatggaac
     1021 tgcattaaca gctggtggta aagtaagtgg aatcgttaag ggtgaaacag tagactttac
     1081 aattactggt agtcaaacta gcgttggtac ttcagataac acttatgaat tggattggac
     1141 aggaagtgca aaagaatcga actacaaaca tggtaaagat tcaattggaa cattaacagt
     1201 aaaagctaag tcaatcgttc cggatggacc agatactcca gatgaaaaga agacgggtat
     1261 tacagtaagt gatcctagtg attctaagta tgatggtaaa gagcatagag aagtattaac
     1321 agttcaggat actaagaaaa atgatgaatt agtagctgaa aaagattact ctgtaactta
     1381 tagtgatgat ttagtaaatg ctggtacagt aacaattact attgaaggta ttggaaatta
     1441 tactggatca ttcacaaaga cttatgaaat cacaaaacgt tcagtaacat taacaagtgc
     1501 tactgcaagc aagacttatg atggtcaagc attaacaagc acatcaatca cagtaagtgg
     1561 tgatggattc gtagagggtg aaggtgctac atataatgta actggaactc aaacagaggt
     1621 tggaaatagt gcaaactcat ttgaatacaa attaaatgaa aatacattag cttctaacta
     1681 cgacattaca aaggttgttg gaacattaac agttacacga gatacaactc cagtaactcc
     1741 agctcctaca cctacaactc caagcgtacc gccagttgtt caaagagtag ttcctactcc
     1801 tactcctgtt cctgaagaaa aggtcgaaga agaacaaacg cctaaggctg aacctaaaga
     1861 ggaagaaaaa gttgaagagg attacactcc aaaggcaaca caagagtact attgggcatt
     1921 aatcaacttg atttgtgcaa tcgtaacagt attattcgga ttattattgt tgatttctaa
     1981 acgtcataaa gatgaagatg acgaagaaga ggatgatgaa acaaaacaac aagcaactac
     2041 aaatgaagac gaagaacaag aacaagaaaa gaaacgtgga ttattcactc gcgtacttgc
     2101 agttctaatc gcaatcgtat ctgttgtatt cttcctagta actgaagaca tgagtctagc
     2161 atggacttgg acagatcaat ggacaatttg gatggttgtt attggattgg tacaaatcgt
     2221 agtattcttc gtaggacgta aatggaaaaa cgttgataac gatgatgatg acgaagaagc
     2281 acaacaagct taataaaagt taataacaca aaaggagtag cctcgtgcta ctcctttact
     2341 gcccaatacc catttcgatt agagcctttg cggattacaa tttcttgttg ctgcaaccta
     2401 tttaatatgc gtttaatagt tgagcgtgat aagtttaatt gatttgcgag ctctatttga
     2461 gtgatttttg ggttgatttt aatgagttgt atgatatcgg gttcattggg tccattgggt
     2521 tcatttactt tgaacccgat ttttttgttg ggcttataga atacattgtt tctatatatg
     2581 atgactttta actcggtttc tccaccaata aatacaggag gttttaaacc acattcttcg
     2641 attgacttaa tgattctagg gatgccgcga ccccagtttt caatgtattt tagatagcgt
     2701 aatgcatacg cggttgcttc attcctaata ttagaatttc cttgtttcat tttctcgatt
     2761 gtttgtccca taactagttt tcctggagaa gttacttcta aacgattatc gaatatcgca
     2821 attttaatac tgtttctatc aatataggat ctgtgtgcaa tggaattcac aattaattct
     2881 cgtatggcat atacgggaat ttcgtatgtt tcttttcttt ctagagattc aaatgaagca
     2941 tgtaaacgta tgtttcgtaa tacaaattta tacgcaaaat caatttgttc atagatgggt
     3001 cctgaataat ctttcatatc aattacttct gttgtatccg tgcctttaaa aagtgcacat
     3061 tgaatacggt ttggcaatgc gggatgtcct gttaataagg cataggcatt agtaggtaca
     3121 atatgattgt ttgattgtgt aagtattccc cacgataata aatcttgttt cgtaatgggc
     3181 gtaactgcat ctttttcaaa ttgagattta gagttttcta atgctttatg ataaagggta
     3241 tcacataagt tttggatact attttcactg atttctaatt cgggacaaat catcttatca
     3301 aaatagcggt tgctaccttc aaacatcaat tctttgatca tattacgatc ggcttttctt
     3361 gttgttccac taacgcgtac ataaacacca tcaaattcac cctctgcttt taaatgatat
     3421 gggcgatccc atccagatgg aacattcaca attaaaatgg ttttatcttc gattgtatct
     3481 aaatcaatgc taggaataat atgaggttca atatttgaaa atattgaatt tgtgatggaa
     3541 tccttgattt taaaaacatc acccgatagt ccgcatattt caaatgaatc tttcttttct
     3601 tcaattccaa aaataatttt tccaccaaaa ccattcgcaa aagctacgac agattttaaa
     3661 tattttttat gttcagcagg aacttctttt tttacttcga tattgttatt ttcgccatat
     3721 ttaatttatt tatcaacata gtattcctcc tttcacacat ccacattatc attataccta
     3781 aaaacccatc ctagtgctta ctagaatggg atctttgtcg ttaatagttt cctgtataat
     3841 tctacaccct tttttaaaat gtcttcatca aaggtgaact tatttgaatg taatggaatg
     3901 gtgtcaccag tacctaaaaa gaagaagata gatggggcta cgtttctgta ataaccaaag
     3961 tcttcactaa taagcacagg actatctagt ttactgacta ggttattatt gtatagggta
     4021 tgaaataatt cttcattatt tagtacaggt ggatagtgat gattgacttt gaaattaact
     4081 tgcaccttgt attttttgat tgcattattc gtgttttgtt caatgatatt aacaatttga
     4141 tcaaatacct ttggatcata atagcgaata gatcctcgta catgagcttg atctgcaata
     4201 atattgaagg cttggccaga atggaaagta ccaaagtgta ataggtggaa tgtattgttg
     4261 tcaaaggctt tttcttcttg ttctaaagac ttttgtagaa tttctacaca agccgttaat
     4321 gcgttgattc cattttgagg ttgggctgca tgacttgcct ttccctttac cagaatttca
     4381 aactcacatc caccagccat catttcatta ggccgtgtat atactttgcc tttttcaagt
     4441 tctggataga tgtgtgttcc gtagatggcg gcaatattaa attgtttgaa gaatccagat
     4501 tcaatgatgg gtttagcacc accgggtttt tcttctcctg gctgaaataa gagtaatacg
     4561 ttgtagttac tttctttggt atatttagct atttctaaaa ccatcgccat atgtccatca
     4621 tgtccgcagg cgtgcataca tccattggtg cttttaaatt ctaaatctgt attttcttcc
     4681 ataggaagag cgtccatatc ggaacgaaaa acaattgttt tttctttctt attgtcaaaa
     4741 aaggcggtta cactattagg aattggatga tcaattgtgc agtcatataa atctaatacg
     4801 ttttcgatat attgtgctgt cttttgtgtg gtaagttcaa tttcagggat ttggtgcaaa
     4861 tttcttctat tttcaatgat tgttggcata aaattctcct tttttggttt attatactac
     4921 ctattttgaa ttgttaaata aatttaacat agatttaaca ttgtaaagat agtatgaaaa
     4981 tccatttaaa tttatattaa gtgcgtaatt atgaaaaggt ggaaaatatg acaacaacaa
     5041 atatattgtc tttattgggt ggattagctt tattcctata tggaatgcag atgatgggaa
     5101 atggtcttga agctgcggct ggagatcgct taaaacaaat tttagagaaa ttaacttcaa
     5161 atcgattttt aggtgtaatt gtaggtgcac ttgttacggc agttattcaa agttcgagtg
     5221 caacaacagt tatggttgtt gggtttgtca acgcacagat gatgacttta caacaagctg
     5281 tatggattat tatgggtgct aacgtcggta caaccattac agggcaattg attgcgttag
     5341 atgtaagtgc tattgcccca ttagttgcct ttatgggtgt tgcattgatt atgtttacga
     5401 aaagcgcaaa ggcaacggat attggacaga tcattgcagg tttgggtatt ctttttattg
     5461 gtatgaatat gatgagtacg gctatgatgc cgctaagaga atcaaaagaa ttcgttgaat
     5521 tagtcactca tttctcaaat ccattagtag gtattttggt gggtgcagga tttacggctt
     5581 tgattcaatc aagttcagcc tctgtaggta ttttacaagc cttagcagct agtggtgtta
     5641 ttggattaga tggggctgta tatgtattgt ttggacaaaa cattggtact tgtattacag
     5701 ccgttcttgc atcaattgga acgagtcgtg aggctaaaca agcgacagct attcacttga
     5761 gttttaatat tattggtaca gtaatattta cattagtatg tatgttcaca ccattggtat
     5821 ctttggtgat tcattggact ccatataatg cgacagcaca gattgcgaac atgcacacat
     5881 tgtttaatat ttgtacaacg ttattattgc ttccgtttgg aaaatatttg gctgaatttg
     5941 cacaacatat cttgcctgtt gaaaacaaag aaacagaaga tgatcgttta ttgtttttac
     6001 agccactacc taagtctaaa aagatgattg gtggtgcagc tatttcggtt aaacaaatca
     6061 atgatgaaac aatgcatatg ttagagcttg cgtatgagaa tgtaaatcag gcatttggac
     6121 agttattaca gtttagtgat aagatagatg aacaaatcta caaaagagag gatactgtaa
     6181 actatttgga taaagtcatt agtgaatata ttagcacggc tttggctaca ccaaacttga
     6241 atgtgaatat ttctcaagct ttgtctagtt attatctaat gttagtggat attgaacgta
     6301 ttagtgatta tgcggtgaat atgaatcatc aggctacaaa acgtcttcaa ggggatatga
     6361 cgatatctga aattgaaatt gtggagcaca tgaaaaagtt gtgtgtcgat atgaaaaatg
     6421 atttgagtga tgtggaagag gcggaacgta aggacgatgt gattgattca cttgtatcaa
     6481 gttggcgcga agcacagatt caagcattaa aacaacagaa atgttcgagt gaagtaagta
     6541 tgatgttatc gcgtattttt acagattttg agcgtattaa tgaccatgcg ttaaatgtgt
     6601 cacaagagtt tgacaagatt aatatagaaa tcccagaata agcagttttt ctgcttattt
     6661 ttttcgtgga ttttgttata atcttgttat gaatattgag aagttattaa atacaattat
     6721 tgaagcaggg aagatgttag tagaaagtgg tgcggaagtc aatcgtgttg aagaaacaat
     6781 ggtcagaatg tgtcggtgct ttgagggaat cgaatatgca gatagctatg tgactttaac
     6841 tggtatcatg ttttcgttaa catatgaaaa taaaacgata acacgtattt gcagggtgca
     6901 tacgggagag gttgatttaa atagaattga tcaaatcaat acgctttcta gaagtatctg
     6961 tacgaatcct atatctgtag atgagttggc aaatgagctt gaaaaaatta agaaaatatc
     7021 tcgctataca tttaaagaga ctatgttttt tggcgcggta ggtgcagctg gatttgggat
     7081 tttctttaat gggactttat tggaaattgt gatgtccttt tttattggaa tcttgattcg
     7141 ttgtataagc tgttttttat ccaagcataa actaaattct ttcttaaata atgcgatatc
     7201 tgcgggatcc gcagcattga gtgctcaatt aattcatcaa gtattgcctc agaccaatgt
     7261 ggatattatg gtgatttcga gttttatgct tttagttcca ggacttgcga ttacgaacgc
     7321 gattcgtgat acgattgcag gagattatgt atctggtgtt gcaagagcag cagatgcatt
     7381 tttatgcgca gttgctattg cggtaggtgc tggttttgtg atgtattttt ggatataggg
     7441 gaatggaaat atggtgtatg taattttaaa atcaggtata tcttcattcc ttgcaagttt
     7501 gggatttggt ttccttttca atatcaaagg aagaaattta tggatagccg gggttgtagg
     7561 tgctattgga ggtgcttgtt ataaggtatc attatattta gggtgttctg aaattctatc
     7621 gaatttttat ggagccttag gtttggcatt agcttcagaa attttagcac gtaaatgcaa
     7681 gactccagtt acaacttttt tggtttgtgc attaattcct ttagttcctg gtggtgggat
     7741 gtatcgtacg atgttggctg ctattcgtgg ggatgctcaa caaacattaa atatgggtct
     7801 tcaaacattg agtattgcgg gtgttttagc acttggaatt ctattggttt ccacaatcat
     7861 gcgtgtgtat tatcaggaaa agagaaaata tttatcaaaa aagtaaaaaa gtacttgcaa
     7921 tcgttaaaat ttatgatatt atatatgagc gtcttgggga tatgggcctg tagctcaggt
     7981 ggttagagcg cacccctgat aagggtgagg tcgttggttc aagtccattc aggcccacca
     8041 tgttttcttt agacgcttat ataatggggt gttagctcag ctgggagagc acctgccttg
     8101 cacgcagggg gtcatcggtt cgatcccgat acgctccacc aattataaaa tttaaactgc
     8161 atatgcagtt tttttttatt ctttttttat tatatgttat aatcataagt aatcggatga
     8221 ttagaaagct gagtagatgt atggaaaaag aattttacga agaatatgaa gatatggaag
     8281 ccggtattga tcaaaaacag gctcttgtcg aagaatcaaa aaaggttgat gaaattgaag
     8341 atttcagcga agcggttcgc tttgtgaatg atttgaaaaa aagatggaga aagactggtt
     8401 ttggtgagtc tttggcagaa gaaaagttaa gagaagaatt tgatgcgaat attgaaaaag
     8461 tatatgaaaa acaaaaggca ttggctcaaa aagtagttga tgtaaaggaa gctttgatta
     8521 aagaagctga aaaaacttct ttatcggatg actttaaaaa ggctactgaa aagatgactt
     8581 ccttaatgga tcaatggaaa gtttctggaa atgccggaaa gaaaacagat gatgaattat
     8641 gggaacgttt caatgcagct cgtcagaagt tctatgatcg taaacatgct aactgggaaa
     8701 atagaacagt tcagtttgaa aatgctaaaa aggtaaaaga agatttaatt gaaaaagcta
     8761 agtcattaca agattctgaa gaatggcaga aaacaagtgc taaatataaa gaattaatgg
     8821 atgcttggaa agctgcagga aatgcaggtc gtgaatttga tgatgattta tggaatgcat
     8881 ttaatgaagc tcgtcaaaaa ttctatgcga aacgtaatga attctatgaa aaattacatg
     8941 cagaacatga cgaaaaatat gcagaaaaac aagctttagt caaggaagca aaatcaattg
     9001 caggcttaca agaatatact cgtgaacaaa ctgcaatgat gaaagaattg tctaataaat
     9061 ggaaatcaat tggtttctgt ggaaaagata aagaagatga aatctggaat gaattcagag
     9121 gtgtcatgga tgaatatttt gctgggctaa aatcggctag cgaaaagcgt cgtgctaact
     9181 ggcgtgatca catggcagaa atcatttctc gtaaagaaga acaaatcgca aaccaaaagc
     9241 gccaaattaa acgtcttcaa gatgatatga caggcttggt aagtgaggct acagtagctg
     9301 acttacaaga tcaagtagaa gataaagaag actttattaa acaacttgaa caagaaattg
     9361 aagacattga aaagaaatta gcagagtagt tgatctactc tctttttctt ggggtttatt
     9421 atatgaattt tcatgaatgt atgaagctgt tttatccaga aacaaaaata atattatata
     9481 atcgaaaaaa tgaggttgta ttagaaggcc aagtaaatga cattcgtaaa gagtgtgaaa
     9541 aatatcataa tgtagaggta acctttgtat cgggtagagc ggatacaacg ggacttaaaa
     9601 tttggctaga tatgtaaaag gaagaaagta tagtgctttc ttccttttcc gttttagtat
     9661 ttcatatcac ttttaattat atcgtgatct tttaatagtt tttcaatcac agtacccttg
     9721 attgtcttaa gaattggttt tttgaataca tttagaggcc caagatcgat ttctaatggg
     9781 gattttccat ccattaattt tacgcttgaa tctaataatt cacgtacatc atatacgctg
     9841 ccccaaactt ctggaacacc acgatcgaca cctaatagtc catatacagc ttccattgcg
     9901 gtacgtactg aatattctgt tgtgaagact gtatctcttg gtgtgtcggc aaattgtcct
     9961 aagaaggcga agtttacaca tccatcagga attacatctg gacgatcccc tttcgttctt
    10021 ggcatgaaga aggcagtgat atatggcatc attgtaggca cgcatcttgc agaatttgta
    10081 gctaattctt caatttgatc tgttggaaca cccaagtgat atagccattc ttctgtgatt
    10141 tctttaccag tacattctct cattggtttc ttaatgtagt caccatctac atctgtaaat
    10201 agtccatata cccaaacgca tactttgttt ggatcttggt ttttgaattg tccttgacga
    10261 ttaatagtcc aactcattaa ccagctcgaa tctctacaag ttacgatacc tccagtaacg
    10321 gtttttccac tacgaggatc acgcttacaa atgtttgtga tataagggat gattttatca
    10381 tctaatgttg ttacagttgc agattcccaa tttgtctttg aaatgtctga acagaatttt
    10441 tctgggtgcc caaaacttgg atcttgtttt gcgatgtttt tccataagct ccagcatcca
    10501 ctagttctaa cttcagcatc tccattaggc gcatggtctt gatcaccata aattgttcct
    10561 tctgtacagc ttccgtttgt cacaaatact aaatcgtttt cagttaatac aatgccagtt
    10621 tctgttccgt ttactttgca ttcgattgtc ttcgcaactt ttttgtcacc tacaaactca
    10681 aattcaacat ttgtaacttc tgtattgaat tggaagtcta cgccatgagc ttctagatat
    10741 ttttgcatag gaagaattaa agattcatat tgattgtaac gagtgaattt taaggcactg
    10801 aaatcaggaa gtcctgagat atggtgaatg aatctttgga agtataattt catttctaag
    10861 gctgagtgcc agttttcaaa tgcaaacata cttctccaat acatccagaa tgttgaatca
    10921 aatacttctt catcaaatac atcttcaatg gtcttatcat ataaatcttc atctggtgtc
    10981 atgaataatt tcataatttc catagcacct ttagtactta aatcaaactt tccatctgta
    11041 tgtgcatctt ttccttggtt gattgttgca cgacacaatg agtagttagg atcatgttta
    11101 tttaaccagt aatattcatc taaaacacta gccccctcga tttctaatga agggatactt
    11161 ctgaatagat cccataaaca ttcgaagtgg ttttccattt cacgaccacc tctcatgaca
    11221 tatccacgag taggatcata gattccatca catgcgcctc cggcaatgtc cattgcttct
    11281 aaaatatgga tgtttttgcc tggcatttgt ccatcacgaa ctaaaaaaca ggcggctgct
    11341 aatgcagcaa gtccactacc tacgatataa gcattcttat tatctacatt tgctggtttt
    11401 tcagggcgag caaatgcttc gtagtttcca ttcgtgtagt aaataccttt agagttcttt
    11461 tcgtattttc ctctttcagt attacgatag tcacttgtaa ctttcttttc ttctttcttt
    11521 ttgtcgctca ctttttttgc agtaaataaa gcagcacctg tagctgcagc agtaaggcct
    11581 gcaatgccta attttatatt attctttgac atatacatgt cctcctttgt tcttatggct
    11641 acataataat ggatgtcaaa attaaaaacc atgaacaaaa aaagcaagat gataattttt
    11701 ttatcatttt gcttcatttg taaagtttga aattgaattt gcgatggaac catgcaaagt
    11761 attggccata ttatggacga tttcatgata atcttcactc atgcctttgc tgatccaatt
    11821 aagcataatg cctacaaaac tatatttgta gaaatcagca ataaattgaa tttgttcttc
    11881 tgtaatattg gttccttgtg ctttttcacg gacgacgcct aaaataaggt cgtgtactgt
    11941 agtaaataaa aaacgttcaa tactatcttg tgttgttgta tggtatacat ttaaaataaa
    12001 tggcttgttt tgaagaacgg cttcaaatat ggatgtaagg ccttcttgcc atgtgtcata
    12061 tgttttattt ccttttagtg catttgttgc atcataaata caagaccatt cgactaggtc
    12121 gtaaatatct ttaaaatgat agtaaaaaga cattcttgaa atgccaatat catctgttaa
    12181 atctttaatg gtaatcttat ctaatggctt ttttaataag aatcttttta gggattgctc
    12241 taaagcaagt ttcgttaagt ttgacatact taaatattat aaaaaaatgg gtctcccgtc
    12301 aattcttagg agaccatgaa cattaaattc tgagtggctg taacaaacag aattttttct
    12361 tgccctagct gtaatagggc tctttgaata ttgtagatgt gcttcttgta agcacactta
    12421 cactatagta ttatcattcg ttaatttaat caaaacattt tgtgaaattt tgggaagaaa
    12481 gagaatctag gtttgcagaa tacaaatatt gatttttgtt tctgcttttt actagcaggt
    12541 ttttaggttc taaagacttt attattttgc gagtcgttgt atcgcttttt tcaagtaatt
    12601 cagttaatgt attcatgttc atataagtat catcaaataa tctattttgg cacagtatga
    12661 agatgcattt tacctgttta tcgttta
//
LOCUS       DBMDWL010000046        12570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1691_length_12570_cov_2.376935, whole genome shotgun sequence.
ACCESSION   DBMDWL010000046 DBMDWL010000000
VERSION     DBMDWL010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12570
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_12570_cov_2.376935"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(225..530)
                     /locus_tag="ACZPDN_04710"
     CDS             complement(225..530)
                     /locus_tag="ACZPDN_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR1297953.1"
                     /translation="MSKTSNVYARIEPETKEQAEAILSKLGIPSSVAINMFYNQIILN
                     NGLPFDVKIPSNKILNEDELTKEELAKEILAAHNEVLKGNTIPFDQFYESLKKDLKL"
     gene            complement(615..1199)
                     /gene="yihA"
                     /locus_tag="ACZPDN_04715"
     CDS             complement(615..1199)
                     /gene="yihA"
                     /locus_tag="ACZPDN_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1297954.1"
                     /translation="MISEFVISAVEKEQWPESDLPEVVVVGRSNVGKSSFINALTNKK
                     KLAYVGNTPGKTRLINFFKIDDTWMLVDVPGYGYAKMSKQMLIKMGKMMDTYFNQREQ
                     ICCVVQLVDARHGATHDDLDMIEMIQSLGIQIVIVATKVDKIPKTKRVRALKDISQQL
                     HLPMKNIFGISSTEKTGFEPVLERINEIVEQTKK"
     gene            complement(1201..3507)
                     /gene="lon"
                     /locus_tag="ACZPDN_04720"
     CDS             complement(1201..3507)
                     /gene="lon"
                     /locus_tag="ACZPDN_04720"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR1297955.1"
                     /translation="MELNTYPLVCTRGVVLYPNQEIVIDVGRDTSVHAVENAQDNYDK
                     KICLFSQKELEQENPATEDIYSVGTLCEIRHIRRFDNFLRVKFRGIKRVKLNKWINGS
                     LSDLVEVEILESEKQDAVEEEALIRMIADELDRMQGQDRFVTKEIVMEISKGMGGEFL
                     SDKAVQGLPLDLERKQKYLETLGVNDRLMMLLQDMAKEKKMSEVEKQINETVKERIDQ
                     GQKDYYLREKMNVIREELGDTVAQDEDAAKIRKRLAENPYPDYIKKKVSDEVSRYEML
                     PMASGETGVIKAYIDWLMDLPWWQQTKDNEDLNAAEATLNADHYGLEKVKERILEYLA
                     VKQMTNSLNAPIICLVGPPGVGKTSLAKSVARALDRKFVKMSLGGVRDEAEIRGHRRT
                     YLGALPGRIIQGMKKAGVTNPVFLIDEIDKMASDYKGDPSSAMLEVLDPEQNSVFSDH
                     YIEEPYDLSKVLFIATANYLENIPPALRDRLEIIELSSYTDAEKVHIAKDHLVPKQLK
                     LNGLKPSQLKIDDDMLLYLIRYYTREAGVRSLERTIATICRKTVLAILKNNKRSIKLT
                     KKLVHEWLGNEKVDYGKKEKKDQVGTVTGLAYTSFGGDILQIEATRFTGKGRLVLTGQ
                     LGDVMKESASIALDYVRTHATEFKIDPKLFEENDVHIHVPEGAVPKDGPSAGVTMTTA
                     LVSCFSNIPVKANLAMTGEVTLRGNVLPIGGLKEKSLAAHRSGIDTILIPKENVKDLD
                     DVPQEVKDAVTFIPCSNVSQVLHNALVK"
     gene            complement(3572..4849)
                     /gene="tig"
                     /locus_tag="ACZPDN_04725"
     CDS             complement(3572..4849)
                     /gene="tig"
                     /locus_tag="ACZPDN_04725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1297956.1"
                     /translation="MTSTWTLKENSTGELEVTVEGEAWKKAQKKALNYAKSHMNLKGF
                     RQGQIPDALVKKQLSSKALYDMASEEVANEALAEGIKEHNIELVARPTLDVKEADDEK
                     AVLVFTCTVLPEVTLGEYKGLDIKKADVEVTEEDVENEVKRVQDRYADWVVREDDDAA
                     QLGDQVVIDFVGTKDGVAFEGGSGENYPLELGSGSFIPGFEEQLVGVKKGEEKDVEVT
                     FPENYQAAELAGQPATFHCTVHEVKYKDLPELNDELIKKLKIENVETVDAYKEKVKTD
                     LTAQKEREAEENFTNELLGKVCDNANVEIPAAMIDAEVDRMYREFEGRMQQSGFTAKQ
                     FLEATHQTEEAIRAQMSPEAAARVKTQLVLEAIVKAESIEASDEDVEKEFTTMSEMYN
                     MEVEKIKSLISPESIKADLANQKALDFIKTSVK"
     gene            complement(4842..5573)
                     /locus_tag="ACZPDN_04730"
     CDS             complement(4842..5573)
                     /locus_tag="ACZPDN_04730"
                     /inference="COORDINATES: protein motif:HMM:NF022868.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3196 family protein"
                     /protein_id="HIR1297957.1"
                     /translation="MNAYYHSLIEEIKKEIDEKSYAQAYELICQELNMPYVPQDALEL
                     LESYKSECKPYLEQKNKSMDFNKLQDYIHGSLEQKIYAISMLENMNLRMCHDEVQVLL
                     DSDLPDEYKGTLIEALMEQKIDDPFDLIKSGLNITFIPSAILPMEQDLSICEASSLFE
                     SWFTNEDPTMVQFCMSLLMQLVLYERPFDQEDKDPYQLAKSVTRLVYDAMQKSDEWPG
                     FVKKQGLEKIEDIELSIEKRGENND"
     gene            complement(5689..7065)
                     /locus_tag="ACZPDN_04735"
     CDS             complement(5689..7065)
                     /locus_tag="ACZPDN_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1297958.1"
                     /translation="MQNPLGYEKESSLLKKFAIPSIISMLVSSLYNIVDQIFIGQGVG
                     FLGNAATNVAFPLTTIALAIALLIGIGSASLFSLYLGEKRPERSSSIAGNGISMSVLC
                     SVIYVVLVLVFLEPLLKSFGATSTIMPLALEYTRITTLGVPFLITTNVISNLIRADGS
                     PKYSMTCMVAGAIVNTILDPLFIFVFHMGVAGAALATVAGQVISFFIAAWYIKSFQHI
                     EFNKSSLRISLNDTKEMATIGMSASLNQIAILFVQIVLNNSLTYYGQFTKFGSDIPLA
                     ACGIVMKTNAILLAIIIGISQGMQPIIGFNYGAQKYERVKKTFKLAISVNLVVSFIGW
                     ALFQFCTSTVLSIFGSGDANYFEFATMFMRIYLMLVCVDGVQMLSSSFFSSIKKAYLG
                     MFLSMTRQVLFLIPLVLILPKFFGLNGILYAAPIADFVAFVVSVICILAEFKKLNELE
                     KNMKVDSL"
     gene            complement(7067..7834)
                     /locus_tag="ACZPDN_04740"
     CDS             complement(7067..7834)
                     /locus_tag="ACZPDN_04740"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1297959.1"
                     /translation="MSEKREGYQFVGWYIDKECTKRLNPGGVLPHSVTLYDKWIPIWY
                     PIEYDVDGGVMSRRNPRYTCIESEELLLYPAFKKGVMFVGWQFNGEIITSLPAGIHEP
                     VILTAIYKSCPVIHFETCGGANYMDVCTDEHGLLGNLKPPVRVGYTFDGWFWDCDYRW
                     RYNLDEPIHESCTLYAHWQVSQYKITYDLNGGLSSRRNPKSFTYFSDTIHLLPATKPG
                     YEFVGWFDERGNELNVICEHSMGNKHLIAQFRKRELN"
     gene            complement(7890..10826)
                     /locus_tag="ACZPDN_04745"
     CDS             complement(7890..10826)
                     /locus_tag="ACZPDN_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOTE conflict system archaeo-eukaryotic primase
                     domain-containing protein"
                     /protein_id="HIR1297960.1"
                     /translation="MKQANDNLTIINDLQKEIQCLKNILNREGISYQEELDQLKNSAN
                     AETIETNQESRIVFPDEITKDMANRFFARFWGRTDVYAKRVENKKTNKAGYYPQCINF
                     WSDGCGKKWNAGFRCQDCHYQKYKALTIDSIMSHLKGNEVIGVYPLHKDNTCRFLVFD
                     FDNHDKDAGNDFANIDDSWKEEVNAMREICQLNGIDPLIERSRSGKGAHIWIFFDKPI
                     SAILARNFGNALLDKGAEQVNLKSFKYYDRMIPAQDSMPKGGLGNLIALPLQGRALKD
                     GNSAFIDENWNAYSNQWNILWSKPKLSQSFIEEKIKEWHGNSDDKPWDSVKEFEKNEV
                     DGKMHIILSNGIYVDTSNLSVSIQNKIRKLARISNPIYYKNQGMGISTFGMYKYIYLG
                     EDHDSGYIQIPRGCYDTLISYNKNAGIQMMIDDERQQGRSIDVTFKGELKPEQNVALN
                     KMIEHDNGILLAATAFGKTVLCADLIATKKVNTLILLESSSLLEQWKDKLQTFLDINE
                     ELPEYTTPKGLIKKRKSLIGTLQGSHDSMTGIIDIAMAGSICKKGKFHKLLNEYGMII
                     VDECHHAASNTESAVLKEIKAKYVYGVTAVDRTDQLDKMNFMLIGPIRHKYSAKEQAK
                     QQGIPRLLYPRFTHVVAPRGMMKDEKNPNEAYSILRDNDLRDQMIIQDIKECIKKKRT
                     PVVLSKYVNHCKKLYKQLEGVADSVFLLIGSQSKKENNHIVELMKSVPEDKTMILVAT
                     GSLVGEGFDLDRLDTLFLAMPVASSSVIEQFTGRIHRLYDKKDTILVYDYVDVHIPMF
                     DRMYGKRLKAYKKSGYELISDTNGIKSESQISQSIYDASNYYDMYSHDLMRAKKNIIV
                     SSPSLSGDRVLEFTKLIKEKMESGVEVTIVSWMPDKIRFGSSNYRMQLLEEMRQSGFY
                     LKSAEDNCEHFAIIDQEIVWYGNINLLGKQDVEDHIMRIQSKEIASELMEMTFGNSLY
                     NYLNNF"
     assembly_gap    11398..11407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12064..>12570
                     /locus_tag="ACZPDN_04750"
     CDS             12064..>12570
                     /locus_tag="ACZPDN_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1297961.1"
                     /translation="MIKILICEDIERDKLHLHQLLDSFLQSNKISYKIDYLENFDVGT
                     NLFLEYDLIFLDIELGDYNGIEIASKIRTYNRSSIIIITSSFKKYLIDGYKIEAKRYL
                     IKPIDEKVFNIEMQSVLSTSFKQQYGFFDKKISQYKIHYKDMLYIEFLNRHTIIHFVN
                     NIEKKTTYP"
BASE COUNT         3977 a         2491 c         1632 g         4460 t
ORIGIN      
        1 tataatttca caggtgtcat aagcaatgtg attccaaaag cattcacaat tccagcaatt
       61 gtcaacatta aagattcgta atacaaggtg ttgggtcttg tttcataagc tcagcacttc
      121 tttttagggc ttcatttaac atactcacac accaagaatc atttcattca caattctttt
      181 agacaattat aaagaataga ctaatccatt cgcattttca aaattcatag ttttagatcc
      241 tttttcaatg attcataaaa ctgatcaaaa ggaattgtat tcccttttaa aacttcatta
      301 tgagccgcca aaatctcctt agctaattcc tcttttgtta gttcatcttc atttaaaatt
      361 ttatttgatg gaatcttcac atcaaaaggt aaaccgttat ttaaaataat ttgattgtag
      421 aacatgttaa ttgcgacaga agatggtatt cctaattttg ataaaatggc ttctgcttgt
      481 tcttttgttt ctggttcaat tcttgcgtat acatttgatg tttttgacat aaaataagct
      541 cctttttttc tttattataa gtcaatgtac ttacatacgc aatcgtttat catacatcat
      601 aataaaagaa cagactattt tttagtctgt tctacaattt cattgattct ttctaagaca
      661 ggttcaaaac ctgttttttc tgtactacta atcccaaaga tgtttttcat tggcaaatga
      721 agttgttgag aaatatcttt taacgcacga acacgttttg tcttaggaat cttgtcaact
      781 tttgtagcta caatgacgat ttgaattcca agactttgaa tcatttcaat catatctaaa
      841 tcatcatgtg tagcgccatg tcttgcatcg acaagttgta caacacaaca aatctgctct
      901 ctttggttaa aataagtatc catcatttta cccatcttga tcagcatttg tttagacatt
      961 ttcgcatagc catatcctgg aacatccacc aacatccatg tatcatcaat cttaaagaaa
     1021 ttgatcaaac gtgttttacc tggtgtattt cctacatagg caagtttctt cttattagtc
     1081 aatgcattga taaagcttga ttttccaaca ttactacgtc caacgacgac aacttctggt
     1141 aaatcagatt ctggccattg ttctttttca acagcactaa tcacaaattc actgatcata
     1201 ctattttacc aatgcgttat gtaacacttg agatacattt gaacaaggaa taaaagtaac
     1261 cgcatctttt acttcttgag gtacatcatc taagtctttt acattttctt ttggaatcaa
     1321 gattgtatca attccagaac gatgtgctgc caatgatttc tctttcaatc caccaatagg
     1381 aagaacattt cctcttagcg taacttcacc agtcatagct aaattcgctt taactggaat
     1441 attagagaaa caagatacca atgccgttgt cattgtaaca ccagcacttg gtccatcttt
     1501 tggtacagca ccttctggta cgtgaatatg tacatcattt tcttcaaata attttggatc
     1561 gattttgaat tcagttgcat gcgtacgaac atagtctagc gcaatactag cactttcctt
     1621 catgacatca cctaattgtc cagtaagaac aagtctacct ttacctgtaa agcgagttgc
     1681 ttcaatttga aggatgtcac caccaaaact tgtataggca agtcctgtta cagttccaac
     1741 ttgatctttc ttttcttttt taccatagtc aactttttca ttacccaacc actcatgaac
     1801 taatttctta gttaacttaa tgctgcgttt gttgtttttt aagatagcca atacagtttt
     1861 acggcaaatt gtcgcaattg tacgctctaa tgaacgaact cccgcttcac gagtatagta
     1921 acgaattaaa tatagtaaca tgtcatcatc aatttttaat tgagatggtt ttaatccatt
     1981 caattttaat tgtttaggca ctaagtgatc cttggcaata tgaacctttt ctgcatctgt
     2041 atagcttgat aattcaatga tttccaaacg atctcttaat gcaggtggaa tattttctaa
     2101 atagttagcc gttgcgatga ataatacttt tgataaatca taaggctctt caatatagtg
     2161 atctgagaaa actgagtttt gttcagggtc taatacttca agcattgcac ttgaaggatc
     2221 tcccttataa tcacttgcca ttttatcaat ttcatcaatt aagaatacag gatttgtcac
     2281 gcctgccttt ttcatgcctt gaatgatacg tccaggcaaa gctcctaaat acgttcttct
     2341 atgtcctcga atttccgctt cgtcacgaac accaccaaga gacatcttca caaatttacg
     2401 atctaatgca cgtgctacac tttttgccaa agatgtttta ccaactccag gaggacccac
     2461 caaacaaatg attggtgcat ttaatgaatt tgtcatttgt ttaacagcta aatattctag
     2521 aatacgttct ttcacttttt ctagaccata atgatccgca tttaaagtgg cttctgccgc
     2581 atttaaatcc tcattatctt ttgtttgttg ccaccaagga agatccatta gccaatcaat
     2641 atacgctttg attacgccag tttctccact agccatagga agcatttcat aacgacttac
     2701 ttcatccgat acctttttct taatatagtc cggatatgga ttttcagcta aacgcttacg
     2761 aatcttagct gcatcctcat cttgagcaac tgtatcccct aattcttcac gaatgacatt
     2821 catcttttca cgaagatagt agtccttctg tccttgatca attctttctt ttacagtttc
     2881 gttgatttgt ttttcaactt cactcatctt tttttctttt gccatatctt gaagcaacat
     2941 catcaaacga tcattcactc caagtgtttc aagatatttt tgttttcttt caaggtctaa
     3001 aggtaacccc tgaacagctt tatcacttaa gaattcaccg cccatacctt tagagatttc
     3061 cataacaatt tcttttgtca caaaacgatc ttgaccttgc atgcgatcaa gctcatctgc
     3121 aatcatgcga attaaggctt cttcttcaac agcgtcctgt ttttcgcttt caagaatttc
     3181 aacttccaca agatcactca agcttccatt gatccattta tttaatttaa cacgtttaat
     3241 tccacggaat ttaacgcgta aaaagttatc aaaacgacga atatgacgaa tttcacacaa
     3301 agtccctaca gaatatatgt cttcggttgc aggattttct tgttcaagtt ctttttgaga
     3361 aaacaaacaa atctttttat cataattatc ctgcgcattt tcaacagcat gtacagaagt
     3421 atctctacct acatcaatca caatttcttg atttggatat aagacaacgc ctcttgtaca
     3481 aactaaagga taggtattta attccataca aaaacctcct tatattaaat atggaaaaag
     3541 acgctaagcg tcttttttcg tttacattat actactttac agaagtttta atgaagtcta
     3601 atgctttttg attagctaaa tctgctttaa tactttctgg agaaatcaat gatttgattt
     3661 tttcaacttc catgttgtac atttcagaca ttgtagtaaa ttctttttct acatcttcat
     3721 cactagcttc aatgctttca gcttttacga tagcttctaa tactaattga gtcttaacac
     3781 gagcagcagc ttctgggctc atttgagcac gaattgcttc ttcagtctga tgagttgctt
     3841 ctaagaattg tttagcagtg aatccagatt gttgcatacg accttcgaat tctctataca
     3901 tacgatcaac ttctgcatcg atcatagctg ctggaatttc aacgtttgcg ttatcacaaa
     3961 cttttcctaa taattcatta gtgaagtttt cttcagcttc tctttctttt tgagccgtta
     4021 aatctgtttt tactttttct ttataagcat caactgtttc aacgttttcg atttttaatt
     4081 ttttgattaa ttcatcattt aattcaggaa gatctttata ttttacttcg tgaactgtgc
     4141 agtggaatgt tgcaggttga ccagcaagtt cagctgcttg gtagttttca gggaaagtta
     4201 cttcaacgtc tttttcttca cctttcttaa ctccaactaa ttgttcttcg aaacctggaa
     4261 tgaatgatcc tgaaccaagt tctaatggat aattttctcc agaaccacct tcgaatgcta
     4321 caccatcttt tgttcctaca aagtcgataa ctacttgatc acctaattga gcagcatcgt
     4381 catcttcacg aacaacccag tccgcataac gatcttgaac acgtttaact tcgttttcaa
     4441 catcttcttc tgtaacttct acgtccgctt ttttgatatc taatcctttg tattcaccta
     4501 aagttacttc aggaagtact gtacatgtaa atactaatac ggctttttca tcatcagctt
     4561 ctttaacatc taaagtagga cgagctacta attcgatatt gtgttctttg attccttcag
     4621 ctaatgcttc gttagcaact tcttcactag ccatatcata taatgctttt gaagaaagtt
     4681 gttttttaac taatgcatca ggaatttgtc cttgacggaa tcctttcaaa ttcatgtgag
     4741 atttagcata gtttaatgct tttttctgtg cttttttcca agcttcacct tctactgtta
     4801 cttctaattc accagtagaa ttttctttca aagtccaagt actagtcatt gttttctcct
     4861 cgcttttcaa tggataactc aatatcctca attttttcaa gaccttgttt tttcacaaaa
     4921 ccaggccatt catcggattt ctgcattgca tcatatacaa gtcttgttac acttttcgca
     4981 agttggtaag gatctttatc ttcttgatca aatggtcttt catataaaac aagctgcatc
     5041 aaaagtgaca tacaaaattg aaccatagtt ggatcttcat ttgtaaacca actttcaaat
     5101 aacgaacttg cttcacagat tgacaagtct tgctccatag gtaagatggc acttggaata
     5161 aatgtaatgt tcaatccgga ttttatcaaa tcaaatggat catcgatttt ctgctccatt
     5221 aaagcctcaa tcaatgtacc cttatattca tcaggtaaat ctgaatccaa taatacctga
     5281 acttcatcat gacacattct taaattcatg ttttctaaca tagaaatcgc atagattttc
     5341 tgttctagac ttccatgaat ataatcttgt aacttgttga aatccatact cttattcttc
     5401 tgttccaaat aaggtttgca ttctgactta taagattcta ataactccaa tgcatcttga
     5461 ggaacataag gcatgttcag ttcctgacaa atcaattcgt acgcctgggc ataacttttt
     5521 tcatctatct ctttttttat ttcttcgatt aaagaatgat aatatgcgtt cacttatacc
     5581 tccatgcgaa acattagtat tctaccataa atatcgacaa taatcaattc tacgcatttt
     5641 taaaatacag aatccaaaga atgtcatctc tagaaaaaaa ctctcctttt atagagagtc
     5701 tactttcata tttttttcta attcatttaa cttcttaaat tcagccaaaa tacaaataac
     5761 ggacactaca aaagcaacaa aatccgcaat tggagccgca tataagattc cattcaaccc
     5821 aaaaaattta ggtaagatca aaactaatgg aatcaaaaat aatacttgtc tagtcataga
     5881 taaaaacatt cctaaatatg cttttttaat ggacgaaaag aagctagacg ataacatctg
     5941 tactccatca acacatacta gcattagata gattcgcata aacattgtcg caaattcaaa
     6001 atagttcgca tcccctgatc caaatattga taatacagtg cttgtgcaga attgaaataa
     6061 agcccagcca ataaatgaaa caaccaaatt tacactgatc gcaagtttga acgtcttttt
     6121 gacacgctca tatttttggg ctccataatt aaatccaata attggctgca taccttgtga
     6181 aataccaatg atgatagcta ataatatcgc attggttttc atcacaatgc cacaggcggc
     6241 taaaggaata tcactaccaa acttagtaaa ctgtccataa tacgtcaatg aattatttaa
     6301 tacgatttgc acaaacaaaa tcgcaatctg atttaatgat gcactcatac caatggttgc
     6361 catttccttt gtatcattta aagaaatgcg taaactactt ttattaaatt caatatgttg
     6421 aaaactctta atataccaag cggcaataaa aaatgaaatc acttgccccg ctactgtggc
     6481 gagggcagcc ccagcgacac ccatatgaaa tacaaagata aatagtggat ctaatatagt
     6541 attcacaata gcacccgcaa ccatacatgt cattgaatac tttggtgatc catctgcacg
     6601 aatcaaatta ctaatcacat ttgtcgtaat caaaaacgga acacccaaag ttgttattcg
     6661 tgtatattca agtgccaaag gcataattgt gcttgttgca ccaaatgatt ttagtagcgg
     6721 ttcaagaaat acaagtacta aaacaacata gattacggag caaagaacag acatactaat
     6781 accattccca gcaatactac ttgatcgttc tggccttttt tcacctagat ataaactaaa
     6841 taagcttgct gaaccaatcc caatcaacag tgcaatcgca agtgcaattg ttgtcaatgg
     6901 aaaagctaca ttggttgcag catttcctaa aaatccaaca ccttgaccga taaagatttg
     6961 atctacaatg ttatatagtg acgatacaag cattgagata atacttggta tcgcaaactt
     7021 ttttaataat gaagactctt tttcataccc taaaggattt tgcatactaa ttcaattccc
     7081 tctttctaaa ctgtgcaatt aaatgcttat ttcccattga atgctcacaa atgacattta
     7141 actcatttcc tctttcgtca aaccaaccaa caaattcata acctggcttt gtagccggca
     7201 acaaatgaat cgtatccgaa aaatacgtaa atgactttgg atttcttcga ctacttagtc
     7261 ccccattcaa atcatatgta atcttatatt gacttacttg ccaatgggca tataaagtac
     7321 aagactcatg aatgggttca tccaagttat aacgccaacg ataatcacaa tcccagaacc
     7381 atccatcaaa ggtatatcca actcgaactg gcggctttaa atttcccaat aagccatgtt
     7441 catccgtaca tacatccata taattggctc caccacacgt ttcaaagtgg ataacagggc
     7501 atgatttata aatagctgtt aatatgactg gttcatgaat gcctgctggt aaacttgtta
     7561 taatttcacc attaaactgc caacctacaa acataacgcc ttttttaaat gccggataca
     7621 acaataattc ttccgattca atgcatgtat atcttggatt acgacgcgac ataactccgc
     7681 catccacatc atattcaatg ggataccaaa taggaatcca tttatcatat aaagtcacag
     7741 aatgaggaag caccccacct ggatttaatc tttttgtaca ctctttatct atataccaac
     7801 caacaaattg gtaaccttct cttttttcac tcatcaaata tcacctgccc attattttat
     7861 cacaaataga aaaacttcta ggaaaatttc tagaagttat tcaaataatt atataaacta
     7921 ttcccaaaag tcatttccat aagttcactt gcgatttctt tactttgaat ccgcataata
     7981 tgatcttcta catcttgttt acctaataaa tttatatttc cataccatac aatttcctga
     8041 tcaataatcg caaaatgttc acaattatct tctgcagatt ttagataaaa accagattgt
     8101 ctcatttctt ctaacaactg cattctataa tttgaacttc caaacctaat tttatcaggc
     8161 atccaagaaa ctatggttac ttcaactcca ctttccatct tttctttgat caatttagta
     8221 aattctagaa ctctatcgcc agataatgaa ggactggata caataatatt cttctttgca
     8281 cgcattaagt catgactata catatcgtaa taattacttg catcatatat agattgagaa
     8341 atttgcgatt cactttttat gccgttagta tccgaaatta attcataacc tgatttttta
     8401 taggccttta atctttttcc gtacattcga tcaaacatcg gaatatggac atctacataa
     8461 tcatatacaa gtattgtatc ttttttatca tatagtcgat gaattcttcc tgtaaattgt
     8521 tctataacgc tactactagc tacaggcatt gctagaaaca aagtatctaa cctatcaagg
     8581 tcaaagcctt ctccaacaag tgatcctgtc gcaaccaata tcatagtttt gtcttcaggc
     8641 acacttttca ttaattcaac aatatgatta ttttcttttt ttgattgact acctatcaat
     8701 aagaaaactg aatctgccac accttctaat tgtttatata gcttcttaca atgatttaca
     8761 tattttgata atacaacagg cgtacgtttt ttctttatac attcttttat atcctgaata
     8821 atcatttgat ctcgcaaatc attatctcgc aatattgagt atgcttcatt tggattcttt
     8881 tcatctttca tcattcctct tggtgcaacc acatgtgtaa accgaggata taacaaacga
     8941 ggaatccctt gttgttttgc ttgttctttt gcggaatatt tatgtcgtat tggaccaata
     9001 agcataaaat tcatcttatc caattgatcc gttcgatcaa cggctgtaac accatacaca
     9061 tacttagctt taatttcctt taaaacagca ctttctgtat ttgaagccgc atgatgacat
     9121 tcatccacaa tgatcatgcc atactcattc aacagtttat gaaatttccc ctttttacat
     9181 atagagccag ccattgcgat atcaattata cctgtcatgg aatcatgaga tccttgtaaa
     9241 gtacctatca atgattttct ctttttgatc aaacctttag gtgtcgtata ttcaggtaac
     9301 tcttcattta tatccaaaaa tgtctgcagc ttatctttcc attgttctaa taaagatgaa
     9361 ctttccaaca aaattaatgt attcactttc tttgtcgcaa ttaaatctgc acaaagaacg
     9421 gtcttaccaa aagcagtagc agctaataaa ataccattat catgctcgat cattttattt
     9481 agagctacat tttgttcagg ttttaactct cccttaaaag taacatcaat acttcttcct
     9541 tgttgccttt catcatcaat catcatttga atcccagcgt tcttattata cgaaattaaa
     9601 gtatcataac aacctctagg aatttgtata tatccactat cgtgatcttc accaagatat
     9661 atatatttat acatgccaaa agtagaaatt cccatacctt gatttttata ataaatagga
     9721 ttactaattc tagccaattt gcgaatttta ttttgaatag atacgcttaa atttgaagta
     9781 tccacataaa taccatttga taaaatgata tgcatttttc catccacttc attcttttca
     9841 aattctttaa cagaatccca aggtttatca tctgaatttc catgccattc ttttatcttt
     9901 tcttcaataa acgactgtga aagcttaggc ttagaccaca atatattcca ttgatttgaa
     9961 taggcattcc aattttcatc aataaatgca ctgttgccat ctttcaatgc tcgtccttgt
    10021 aatggcaatg cgattaaatt tcccaggcca ccctttggca tactatcttg tgctggtatc
    10081 atacgatcat agtatttaaa tgattttaaa ttcacttgtt ctgccccttt atccaataaa
    10141 gcatttccaa aatttcttgc caaaatagct gaaatcggtt tatcaaagaa aatccaaata
    10201 tgtgctcctt tacctgatct agagcgttca atcaatggat caattccatt taattgacaa
    10261 atctcacgca tcgcattaac ttcttctttc caactatcat ctatattcgc aaaatcattt
    10321 cctgcatctt tatcatgatt atcaaaatca aagactaaaa aacgacacgt attatcttta
    10381 tgcaatggat aaacaccaat cacttcattt ccttttaaat gtgacataat actatcaatt
    10441 gtcaaagctt tatacttttg gtaatgacaa tcttggcaac gaaatcctgc attccatttc
    10501 ttaccacaac catctgacca aaaattaata cattgtggat agtatcccgc tttatttgtt
    10561 ttcttgtttt ctactctttt tgcgtatacg tctgtacgac cccaaaaacg tgcaaaaaac
    10621 cgatttgcca tatcttttgt gatttcatct ggaaaaacaa tacgactttc ttgattcgtt
    10681 tcaatagttt ctgcatttgc agaattcttt aattgatcca attcttcctg ataagaaatt
    10741 ccttctcgat tcaaaatgtt cttcaagcat tgaatctctt tttgtaagtc atttattata
    10801 gtcaaattat cattcgcctg tttcacaaaa ccacctactt ttttcaatac tattttaatt
    10861 gaattcacaa ttaaaaaaaa cacccaagac tccgttagtt agacggagag gagtgttttc
    10921 agaacaaatc tgcaagtagt agtcttgtag tagttctatt catataatac tccttttcct
    10981 ttccttctgt gttaacaaat aatgataaaa taccaattaa cataattgtg tcctatcaag
    11041 attttatata caacaatctt agaaaggttc atagaggatc aaatgcgtat gtattactta
    11101 ttattcactg tacaattcat cttatttttc attgcactta cagattattc aaaatggaaa
    11161 ctaatttcat ttttcaccat tactgcagta ttgattttat atagtatcta tacacatgat
    11221 acagcgcaaa tcatcatgtg tattttggat atagttttca ttggtttcta ttggttttac
    11281 aagaaaggac gctaaataat agtcaggaat caccattcaa tatacttttt aatttccatt
    11341 tttgttgcta tctgttcctc taataacctt tctaagattg ttgtatataa aatcttgnnn
    11401 nnnnnnncgg ttgtatctac atatgtttga agaaatttat tttcactaaa tttgtttcta
    11461 ttttcatttt tcaaattgtt cttttttgta ctatgtagtg tttgaaactt tgatactttt
    11521 tttatttaat tatgagttta ttaactttag tgctttgaat aaatttttta aaatctactt
    11581 ctggcattct atatactcct tatctttttt agcattaaat tgacgcattc atattttata
    11641 cttattatgt cccattttta tattttaata tacatacctt tgcccctaca tctccttgcc
    11701 tttaaaaaag tagcaagcat gtcatttgtc tgacgccaaa cgaccaaact ttttagggtt
    11761 gcacccctaa gaccccatgc ctccgacgga ttctaaaata aaaaagcgga aaatgaatct
    11821 acacacacta aaatatacat gcattttctc ttttatattt tatgtaatac tttatgaatg
    11881 gtggcaatat tgccacatta gcttttttga atgattccca aaaagtcaaa tatctgatta
    11941 tttatttaca tgagtccatt agttaaaagt taaaaacttt aattctttta tagctgtttt
    12001 ataatttcat acaaaagtat aaataagtaa acaaatggtg taaaataata aatgggaggc
    12061 ataatgataa aaatattaat ttgtgaagat atagaaagag ataagctaca cttacatcaa
    12121 ttactagatt cctttttgca atcaaacaaa atatcatata aaattgatta tcttgagaac
    12181 ttcgatgtag ggaccaactt attcttagaa tatgatttga tttttttgga tattgaacta
    12241 ggagactaca acggcataga aatcgcatca aaaataagaa cctataatcg tagttctatt
    12301 attataatca cttctagctt taaaaaatat ttaatagatg gatataaaat agaagcgaaa
    12361 agatatctta ttaaaccaat tgatgaaaaa gtatttaaca tagagatgca gagtgtctta
    12421 tcaacttcat ttaaacaaca atacggtttt ttcgataaaa agatatctca atataagatt
    12481 cactataagg atatgttata catagaattt cttaatcgac atacgattat tcattttgtt
    12541 aataatattg agaaaaaaac tacatatcct
//
LOCUS       DBMDWL010000047        12501 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1698_length_12501_cov_2.993387, whole genome shotgun sequence.
ACCESSION   DBMDWL010000047 DBMDWL010000000
VERSION     DBMDWL010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12501)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_12501_cov_2.993387"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(182..790)
                     /gene="rpsD"
                     /locus_tag="ACZPDN_04755"
     CDS             complement(182..790)
                     /gene="rpsD"
                     /locus_tag="ACZPDN_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR1297962.1"
                     /translation="MSRNTGSIWKKSRRLGFSVLETGEELTRRDTIPGQHGSPRRPKL
                     TNYGIQLQEKQRIRHTYQLSEKQFYNTYLKASKKAGSAGENFLVMLESRLDNIVYRMG
                     FARTRRASRQLVNHGHVLVDGKKVDIPSFQVKPGQVIEIRERAKNLSVINEALEAATA
                     TVDFVEVNKDAKKGTYVRYPERKELFTAQEINELLVVEFYNR"
     gene            1054..2829
                     /locus_tag="ACZPDN_04760"
     CDS             1054..2829
                     /locus_tag="ACZPDN_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005940 - septin ring [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HIR1297963.1"
                     /translation="MDSVLNFFRSVYAFIKNQLSVTVIIYLCIAILVLILVWIIMRMI
                     KRKKASKRLSDLEIEMNEIRNNSLAYKFNKASAFARVNDDIMDKVKKLTPKYDTCQNN
                     LSACDDLFNEADDYVSRHRLRKSMRAMDDLETMMDETKERIRIVTQSLDHILARETEV
                     RESANALKERFRNVKTVYQDNRSSFYGSVNYVDSCLEEIENEFSNFEEWMFASEFNKA
                     KEEQEKISKMVDEISSKIAAFPGLYEKAKNVLPRAINEVRLHIKELQEKDIDLSYLEP
                     EEKLTTIQNALASTIDQLDAGEFELAQENLEIIGDHILALQDDVTNEKQAYEEIHVSL
                     QSNLDIVNVIGQELEEIMALYANIKDRFGLEDWTHRFSLAKIQMEDLEKRRDEIQNEL
                     KQNQRPSVDVVHGYRKFSEDVNEFHDQVKDMKEMLVGASSDESRAKKQLTKLQLILNE
                     VRLNTVTRHLPSISSQFSADLKEGERLIQRVRVVLDHSPLDVQTLNADLQDAIDFVYK
                     LYNNANNLVGVAVMVENAIVFGNRFRSTHAQMDSDLTRAELCFQNGEYTRALKIAIQA
                     IENMHPGIYEKLVAQKDPAVMNQVQ"
     gene            2829..3959
                     /locus_tag="ACZPDN_04765"
     CDS             2829..3959
                     /locus_tag="ACZPDN_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1297964.1"
                     /translation="MIYFDNASTTSVRTEVRRVYGQMLETCYGNPDSLHAIGRQASTL
                     MEKSRSNIASMLHVEPSEIIFTSAASESNTLAIVGYALANEHRGHHVLTSNVEHSSVR
                     HAMEFLKRFGFEVEFLPINEEGIVTPECLKAHMRKDTILVSVMHVNNEMGAINPILEL
                     EKIVHQNPTCVYHVDCVQSFSKIDIPFDKLDMATISAHKIHGLKGSAILMKKKKIQLV
                     PIIQGGQQEQGLRGGTENAPANIVLAKTIRLALEEQSSAYAHVSKINQYLREELSKID
                     GAHIHSPENAIPYILNISFDQITSEVLLNALDQKGICVSAKSTCSSQNTNASEVLLAM
                     HKSEFDAIHGIRLSFSYENTLEEAKYFIETCKEIIENYGLSL"
     gene            3943..5133
                     /gene="thiI"
                     /locus_tag="ACZPDN_04770"
     CDS             3943..5133
                     /gene="thiI"
                     /locus_tag="ACZPDN_04770"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1297965.1"
                     /translation="MAYRYNHILIRYGELSLKGKNRNNFIKALYDNVRKALKAFPTLE
                     FEKQHDRMYIYLHDEDSKQVSDILAKVFGISSFSLAIKVEPDLDQIIDACYESLDLTT
                     PKTFKVAARRSDKLFPYISDEINRKVATKILKNSDWKVDVHNPDVKIVVEVHQDAAYI
                     MTDRIQGAGGYPVGVGGKAMVLLSGGIDSPVACYLMMKRGVHIECIHYASPPYTSQNA
                     QEKVMELARLVSGYQGDVLVHVVPFTDLQLAIYQNADESYAITLMRRMMMRIATGLAQ
                     KRHAQAIATGESIGQVASQTLESMLAINDVTNMPIIRPVVCMDKVEIIDLSKKIGTYE
                     TSILPYEDCCTIFTPKNPVTKPRVDKCEKYEGKWDFDKMVQDCIDNTEDIWVHPVKVE
                     EDLF"
     gene            5185..5946
                     /locus_tag="ACZPDN_04775"
     CDS             5185..5946
                     /locus_tag="ACZPDN_04775"
                     /inference="COORDINATES: protein motif:HMM:NF025217.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR1297966.1"
                     /translation="MLICPVCKEKLIKENKTFRCVNNHSFDCAKQGYVNLSRKQTKNH
                     GDNALMVKARTDFLEKDYYDFMRQYVKKKVKGNVYLDAGCGQGYYTKEIGANFEQSYG
                     IDLSKEAILHASKQDKHTQYIVGSLFDMPFSDESIDCVTSIFVPLGQDEIYRILKENG
                     YWIVVGPGPKHCFELKEVLYDTPYENEMPEIQEQYDMVSQKIIKETKMVDDVWALLEM
                     TPYRYKTSQAGLDRVRELERLEVTFEFVVTVYKKI"
     gene            5943..7559
                     /gene="rlmD"
                     /locus_tag="ACZPDN_04780"
     CDS             5943..7559
                     /gene="rlmD"
                     /locus_tag="ACZPDN_04780"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1297967.1"
                     /translation="MKTTIKKWGINGEGIAFHEGKPVFIENAIPEEVIEFDIKRDEDT
                     YLIGEMTRLVEQSSKRRYPLCPIWKECGGCALMHVKYPAQCKMKEGNLKETLIKYADY
                     NGRILPILKNPEPLSYRNSCKLPFGEEYGELYTGMYERDTNHFQKMERCYVHSKLLEQ
                     TRQAVLKIMNEHHLKMYDDKKKTGYRTLVMKEFDSKIQIIFVTGKEDLSEEFIHDVTQ
                     LEGVVSIWQSVKTETKREVFGDMRFIWGEEKMHLQLEDIQLSLLPRSFFQLNTKQSVN
                     LYNVVKDWMPRSKTTVEAYSGIGAISLFVKDKAQEIIGVESIQDAVDNANENAILNHA
                     DNVSFICEDAGIALAHLVQEKDVDTLIVDPPRSGLDDTMKETILKSKIKTMIYVSCNP
                     ATLAKDLGVLKKEYRIVKIQPVDMFSQTPHVESVTLLVRNDDYRPRRRFDDRKDRDYK
                     PRRDGDFKPRRRFDDKKDGDFKPRRRFEDDKPRRRDGDFKPRRRFDDDKPRRRFNDDK
                     PRRRFDDRKDGDFKPRRRFNDDKPNRRYGK"
     gene            7909..9831
                     /locus_tag="ACZPDN_04785"
     CDS             7909..9831
                     /locus_tag="ACZPDN_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1297968.1"
                     /translation="MNNFPHLFEPIEIGKTTVKNRIFMPPISTNMADKGYVTDDLVAH
                     YSARAKGGVGLIVTEVVTVEPTYVYLPGDMSIYDDSFIPGWKKLVDGVHQYGTKILAQ
                     LFHPAYMAFPVPGTPQLIAPSCVGPYYAQSAPRPATIEELHTIVDQFGQAAHRVQIAG
                     GDGVEIQCAHAHGLLGGFLTPLYNKRTDEYGGNIDGRLRLCLEVIASVRKYCGDDFII
                     DVRISGDEYSEGGLTLNDQIYVCKQLEKAGVDFIHVSGGNTIKRGSSMPAPGTSPAPH
                     AHSSEEIKKHVNIPVATVARINEPWMAEELIANEKCDMCMIGRPNLCDSEFANKAFEG
                     KVDDIRPCIGCGRCLTGIMMGKRIGCTVNPSVEDDTIEPAETKKKVLVIGGGPAGMEA
                     AYVAKKRGHEVVLCEKSNELGGQLRLAAVPIAKQELCKVIKFMIRRLDHEGVEVRLNT
                     EVTKEMLENEFKDYEIVCSSGATPKEIPPFKVFKNWMTADDVLSGKKYPGRNIVVLGG
                     GSVGCETADYLAPLINDLFPMNRKITLLEMTNNLMPGEGGAAKSRLTQRLMQKGVRIE
                     LNAQVTSVDENTITYVKEGVEHKLTEADTLIFAVGYAPKKVEVEAENVHYIGDCEKVG
                     TLKDAITNAHEIAKNI"
     gene            9840..11843
                     /gene="bilR"
                     /locus_tag="ACZPDN_04790"
     CDS             9840..11843
                     /gene="bilR"
                     /locus_tag="ACZPDN_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase, long form"
                     /protein_id="HIR1297969.1"
                     /translation="MTSKLLQPIQVGNLTFKNRIMFPPLTTGYEERDGSIGPRSLAFY
                     TRLAQGGCSYIVIGDVAPVRTASPTPKLYDESQIEMYKKLADALHEYDCKVALQLFHP
                     EYDVQGVGKMIMEAGIAGQLSAKAKAANDVEEAEKQQKICDELTKGAYAKLHHDMQHF
                     VTEASVEQLTAIKNSIAQCARKAQKAGIDAIEIHGDRLLGSLCSTLLNHRTDNYGGSL
                     ENRTRYALEVLQAIKEAAPSMMVEYKLPIITVNPDGSLRGKGGLLEDEAVEFAKMLDA
                     AGIDMIQVAQANHTGNMGDTIPPMGAVPYNWTLGACEKVKAVVSCPVATVGRVVSVEA
                     GEKILEDGTADMIGYGRSLLTDPDIANKVDSGECIRECLNCNKGCVDAIQSRRYLSCV
                     LNAENGDEETIFIKPCTETKNVAVVGAGLAGLEAARVAYKRGHTVTVFEKSDRLGGQI
                     NIASVPPRKDEILRSVQYYEKFLPGKVDIQLNHEPTMEELNGFDYVILAIGAHNMDLP
                     MPVENSNVVSSWDILNGQEVSGKCVVLGGGLVGAETAEYLANKGLEVSIVEMMDKIAA
                     QESETVLPLMEADFKEHNVQKFVNTRVSEIKDNVIYAENTKDETNVEITADTIVNALG
                     SKKNVFDDSKLSVPFTYVGDCSGERTADIASAIRTGYKAANEI"
     gene            12046..>12501
                     /locus_tag="ACZPDN_04795"
     CDS             12046..>12501
                     /locus_tag="ACZPDN_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1297970.1"
                     /translation="MNFSSLQSSVQKFGMNQALKYLEKDPETNIPKLMNLVDKVMPEG
                     WYGSQRKVIREQIDKKGVWYDYILKLYDLDADVRQTVFKNFIFNASLNGSTKQEELSE
                     KYNCNIPWAVLLDPTSACNLHCTGCWAAEYGHQLNLSLDDIDSIIRQGKE"
BASE COUNT         4282 a         1826 c         2570 g         3823 t
ORIGIN      
        1 gatgaaaaat atattgacag aagcattggt gaattcagct actaatgctt cttttatttt
       61 ttagttatac aaattctgca aagttaaggt agtaaaacaa agagaaatac acaaaataag
      121 aaaacacatc cacaaaaaaa gaagcgaatc aagtcgcttc tttctttttt tatgtctaag
      181 attaacggtt gtagaattct actactaaca attcgttgat ttcttgagct gtgaatagtt
      241 ctttacgttc tgggtaacga acataagtac ctttcttagc atctttgttt acttctacaa
      301 aatcaacagt tgctgttgca gcttctaaag cttcgttaat tacgcttaag tttttagcgc
      361 gttcacggat ttcgataact tgtcctggtt ttacttggaa actagggata tctactttct
      421 taccatctac taatacgtgt ccgtggttta ctaactgacg gctagcacga cgagtacgtg
      481 caaatcccat acgataaacg atgttatcta aacgagattc taacattact aagaagtttt
      541 caccagctga accagctttt ttacttgctt ttaagtaagt gttgtagaat tgtttttcac
      601 ttaattggta agtgtgacga attctttgtt tttcttgtaa ctggataccg tagtttgtta
      661 attttggacg acgaggtgaa ccgtgttgtc ctggaatagt atcacgacga gttaattctt
      721 caccagtttc taaaactgag aaaccaagac gacgagactt tttccaaatg cttcctgtgt
      781 ttcttgacat aatttttcct cctattgttt tatatcattc ataaaacaat aacagtacaa
      841 atctttaaag gttgtgttaa caatgatatt ttccgctatc agctattagc ttactcatat
      901 cgcttagtgc tgtaacgcat atcctttaac tttgtatgct gctatcaatt tatgcctatg
      961 tatcatatca aagaacatga ataaaatcaa gaaaaggatg caatgatttt gaagttttgc
     1021 tataataaga atggtttgca aggaggtacc actatggata gtgttttgaa tttctttcga
     1081 tcagtatacg cattcataaa aaatcaactt tctgtgactg taatcatcta tctttgcata
     1141 gcgattttag tcttgatctt ggtttggatc atcatgcgta tgatcaaacg taaaaaggcg
     1201 tcgaagcgac tttcagatct tgaaattgaa atgaatgaaa ttcgtaacaa ttccttggcc
     1261 tataaattta acaaagcatc ggcattcgca agagtgaatg atgatatcat ggataaagtt
     1321 aaaaaattaa ctccaaaata tgatacttgt caaaataact taagtgcatg tgatgatttg
     1381 ttcaatgaag cagatgatta tgtttctaga catcgtttgc gtaaatcaat gcgtgcaatg
     1441 gatgatttag aaacaatgat ggatgaaaca aaagaaagaa ttcgtattgt tacgcaatct
     1501 ttggatcaca tcttagctcg tgaaacagaa gttcgtgaat cggcaaatgc tttaaaagaa
     1561 cgtttccgta atgtgaaaac tgtttatcaa gataatcgtt cttcattcta tggatcggtt
     1621 aattatgtag attcatgttt agaggaaatc gaaaatgaat tctctaattt cgaagaatgg
     1681 atgtttgctt ctgaatttaa taaggcaaag gaagaacaag aaaaaatctc aaaaatggtg
     1741 gatgagatca gctctaaaat tgcagccttc cctggactct atgaaaaggc caagaatgta
     1801 ttgcctcgtg caatcaacga agttcgtttg cacatcaagg aattacaaga aaaagatatc
     1861 gacttatctt atttagaacc agaagaaaaa ttaacaacca ttcaaaatgc attggcaagc
     1921 acaattgatc aattggatgc tggtgaattt gaattggcac aagaaaactt ggaaatcatt
     1981 ggggatcaca tcttagcttt acaagatgat gtgacgaatg aaaaacaggc atatgaagaa
     2041 attcatgtga gtcttcaatc aaatttagat attgttaatg tcattggtca ggaacttgag
     2101 gaaatcatgg cactttatgc caatatcaaa gaccgttttg gtttagaaga ttggacacac
     2161 cgcttctctt tggcaaaaat ccaaatggaa gatttagaaa agcgtcgtga tgaaattcaa
     2221 aatgaattga aacaaaatca acgtccaagc gtagatgtag ttcatggtta tcgtaaattt
     2281 tcagaagatg ttaatgaatt ccacgatcaa gtcaaggata tgaaagaaat gctagtggga
     2341 gcaagtagtg atgaatcacg tgctaaaaaa caattgacta aattacaatt gattttaaat
     2401 gaagtgcgtc taaatacagt aacgcgtcat ttacctagta tttcatctca attttctgca
     2461 gatttaaaag aaggcgaacg cttgattcaa cgtgttcgtg ttgtgttgga tcactcacca
     2521 ttagatgtac aaacattaaa tgcagatttg caggatgcca tcgactttgt atataagtta
     2581 tacaataacg caaacaattt agtcggtgtt gctgttatgg tagaaaatgc gattgtgttt
     2641 ggtaaccgtt tccgtagtac gcatgcacaa atggattcgg atttaactcg tgcagagtta
     2701 tgcttccaaa atggagaata tactcgtgca cttaagattg cgattcaggc aatcgaaaac
     2761 atgcacccag gaatttatga gaaactagtt gctcaaaaag atcctgctgt tatgaatcag
     2821 gtgcagtaat gatttatttt gataatgctt cgacaacctc tgtacgtaca gaggttcgtc
     2881 gtgtttatgg gcaaatgtta gaaacttgtt atggcaatcc agatagtttg catgcaatag
     2941 gacgtcaagc aagtacgctc atggaaaaga gtcgatcaaa tattgcttct atgcttcatg
     3001 tagagcctag tgaaattatt ttcacttcag ctgctagtga atccaacaca ttagctattg
     3061 ttggatatgc tctagcaaat gaacatagag gtcatcatgt tctaacaagt aatgttgaac
     3121 attcaagtgt tcgtcatgcg atggagtttt taaagcgttt tggctttgaa gttgaatttc
     3181 ttccaattaa tgaagaaggt attgtaacgc cagaatgttt aaaagcgcat atgcgtaaag
     3241 atacgatttt agtttctgtt atgcatgtga ataatgaaat gggtgccatc aatcccattt
     3301 tagaattaga gaaaattgta catcaaaatc caacttgtgt ttatcatgtg gattgtgtac
     3361 aaagcttctc aaaaattgat attcctttcg ataaattaga tatggcaacg atctctgcac
     3421 ataagattca tggtctaaag gggagtgcca ttttaatgaa gaaaaagaag attcagcttg
     3481 ttccaattat tcaaggtggt caacaagaac aaggtcttcg tggaggaaca gagaatgcac
     3541 cggccaatat tgttttggca aaaacgattc gtttggcttt agaagaacaa tctagtgcgt
     3601 atgctcatgt ttcaaaaatc aatcaatatt tgcgtgaaga attatctaaa attgatggtg
     3661 cacatattca ttcacctgaa aacgcgattc catacattct aaatatttca tttgatcaaa
     3721 tcacaagtga agtcttattg aatgctttgg atcaaaaagg gatttgtgta tccgcaaaaa
     3781 gcacttgttc ttcacaaaat accaatgctt ctgaagtctt attggctatg cataaatcgg
     3841 aatttgatgc gatacatgga attcgcttat cattttctta tgagaatact ttagaagagg
     3901 caaaatattt tatagaaaca tgtaaggaga ttatagagaa ttatggctta tcgttataat
     3961 catatcttga ttcgttatgg cgaattgagt ttaaaaggaa aaaaccgtaa caactttatt
     4021 aaggcgcttt atgacaatgt tcgtaaggct ttaaaagcat ttccaacatt agaatttgaa
     4081 aaacaacatg atcgtatgta tatttacctt catgatgaag attcaaaaca agttagtgac
     4141 attttagcaa aagtctttgg gatttcatct ttctcattgg ctattaaagt ggaaccagat
     4201 ttagatcaaa tcattgatgc ttgttatgaa tctttagatt taacaacgcc aaaaacattt
     4261 aaggtagctg cacgtcgtag cgataaatta ttcccttata tttcggatga aattaatcgt
     4321 aaggtagcca ctaaaatttt aaagaacagt gactggaaag tagatgtgca taatccggat
     4381 gttaaaattg ttgttgaagt tcaccaagat gcagcctata ttatgacaga tcgaattcaa
     4441 ggtgctggag gatatccagt tggtgttggt ggaaaggcca tggttttatt gagtggcgga
     4501 attgattctc cagttgcctg ctacttaatg atgaagcgtg gtgtacatat tgaatgtatt
     4561 cactatgcat ctcctccata tacttcacaa aatgcacaag aaaaagttat ggaactggca
     4621 agacttgtat ctggttatca gggggatgtt ttagttcatg ttgttccatt tacagatctt
     4681 caattggcaa tctatcaaaa tgcggatgaa agttatgcga tcacattaat gcgtcgtatg
     4741 atgatgcgta ttgcaacagg cctagctcaa aaaagacatg cgcaagcaat cgctactggt
     4801 gagtcaattg gacaggtcgc aagtcaaaca ttagaaagta tgcttgcaat caatgatgtc
     4861 acaaatatgc caattattcg tccagttgtt tgtatggata aagttgagat tattgatcta
     4921 tctaaaaaga ttggaaccta tgaaacaagt attcttcctt atgaagattg ttgtacaatt
     4981 tttacaccta aaaatccagt cacaaaacct cgtgttgata aatgcgaaaa atatgaggga
     5041 aaatgggatt ttgacaagat ggtacaagat tgtatagata atacagaaga tatttgggta
     5101 catccagtta aggttgaaga agatttattc taagagaagt gaaaattaag cacttctctt
     5161 tttcttttat gttataatct ttctatgttg atatgtccgg tgtgtaaaga gaaactaata
     5221 aaagaaaata aaacatttcg atgcgtaaat aatcactctt ttgattgtgc aaaacaaggc
     5281 tatgtgaact tatctcgtaa acagacaaag aatcatgggg acaatgcttt aatggtcaaa
     5341 gcgcgtacgg attttttgga aaaagattat tatgatttta tgcgtcaata tgtaaagaaa
     5401 aaggtaaaag gaaatgtgta tttggatgct ggctgcggtc aaggctatta cacaaaagag
     5461 attggtgcaa actttgaaca gagttatggc atcgatttaa gtaaggaagc gatacttcat
     5521 gcatctaaac aagataaaca tacacaatat attgtgggga gtttatttga tatgcctttt
     5581 tcagacgaga gtatagattg tgttaccagt atttttgtgc ctttaggcca agatgaaatc
     5641 tatcgtattc taaaagaaaa tggatattgg atcgttgtgg gtccgggtcc taagcattgt
     5701 tttgaattaa aagaagtatt gtatgacaca ccctacgaaa atgaaatgcc tgaaatacaa
     5761 gagcaatatg acatggtgag tcaaaaaatc attaaggaaa cgaaaatggt tgatgatgta
     5821 tgggctttgt tggaaatgac accatatcga tataaaacaa gtcaagctgg tcttgatcga
     5881 gtaagagaat tagaaaggtt agaggttacg tttgaatttg tcgtaactgt gtacaaaaaa
     5941 atatgaaaac aacaataaaa aaatggggaa tcaacggtga aggaattgca ttccatgaag
     6001 gcaagccagt atttattgaa aatgccattc ctgaagaagt aatcgaattt gatattaaaa
     6061 gggatgaaga tacatacttg attggtgaaa tgactcgttt ggtagaacag agctcaaaac
     6121 gtcgttatcc tttatgccca atttggaaag agtgtggtgg atgtgccttg atgcacgtga
     6181 agtatccggc tcaatgtaag atgaaagagg gaaatctaaa ggagacatta attaagtatg
     6241 cggattataa tggtcgtata ttaccgattc taaaaaatcc agaaccattg tcatatcgta
     6301 atagttgtaa acttcctttt ggtgaagaat atggtgaatt atatacagga atgtatgagc
     6361 gtgacacaaa tcatttccaa aagatggaaa gatgttatgt acactcaaaa cttttagaac
     6421 agacgcgtca ggctgtttta aaaatcatga atgaacatca cttaaaaatg tatgatgata
     6481 aaaagaaaac aggttatcgt acgttggtca tgaaagaatt tgattctaag attcaaatta
     6541 tttttgtgac gggaaaagaa gatttaagtg aagaatttat tcatgacgta actcaattag
     6601 agggtgttgt ttcgatttgg caaagtgtaa aaacagaaac aaaacgcgaa gtatttgggg
     6661 atatgcgttt tatttgggga gaagaaaaga tgcatcttca attagaagat attcaacttt
     6721 ctttacttcc aagatctttc ttccagttaa atacgaaaca atccgtgaat ctttataatg
     6781 tagttaaaga ttggatgcca agatcaaaga ctacggttga agcctatagt ggtattggtg
     6841 ccatcagttt atttgtgaaa gataaggcgc aagaaattat tggtgttgaa tccattcaag
     6901 atgcggtcga taatgccaat gaaaatgcga tattaaatca tgcagacaat gtatctttca
     6961 tttgtgaaga tgcgggtatt gctttagcac acttagtgca agaaaaagat gtggatacat
     7021 tgattgtgga tccgcctcgt tctggtttgg atgatacaat gaaggaaact attttaaaat
     7081 caaagatcaa aacgatgatc tatgtatctt gtaatcctgc gactttggct aaagacttag
     7141 gtgtattaaa gaaagaatat cgtattgtca agattcaacc agtcgatatg tttagtcaaa
     7201 ctccacatgt agaaagtgtt acgctacttg ttcgcaatga tgactataga ccaagacgtc
     7261 gttttgatga tcgaaaagat cgtgactaca aaccaagaag agatggcgat ttcaaaccta
     7321 gaagacgttt tgacgataaa aaagatggag acttcaaacc aagacgccgt tttgaggatg
     7381 ataagccaag aagaagagat ggtgacttta agcctagacg tcgttttgat gatgataagc
     7441 ctagacgcag attcaatgat gataaaccaa gacgtcgttt tgatgatcga aaagatggtg
     7501 actttaaacc aagaagacgt tttaatgatg ataaaccaaa tcgtagatac gggaaataaa
     7561 acactaaggg atttcagtta aactgaagtc cttttcttat tgattggaaa tttgttttgg
     7621 agaatctcgg tagttcagtg atgagagttt caaatggaga tggttcgaat tggatttgat
     7681 tgtgatattc aatgataaaa tgttatatta aaacattgtt aaggatacct aactaaaata
     7741 agttcacaaa ttaactaaat agatagtttt atgttaacaa atgcgttata atagtatcag
     7801 cgcattcttt ttatttttta attcataata atatacaaga aaagagatag gctttttgcg
     7861 tccatttgtg aataaaataa caagaatcta tgaggaggag tttagaaaat gaataatttt
     7921 ccacatttat ttgaaccaat tgaaattgga aaaacaactg taaaaaaccg tatttttatg
     7981 ccacctattt caacgaacat ggccgataaa ggatatgtta cagatgattt agtagcacac
     8041 tattctgcac gtgcaaaagg tggagttgga cttatcgtta ctgaagttgt aactgtagaa
     8101 ccaacgtatg tatatcttcc aggagatatg tcaatttatg atgatagttt tattccagga
     8161 tggaaaaaat tggtggatgg cgtacaccaa tatggaacta aaatcttagc acaattgttc
     8221 cacccagctt atatggcatt cccagttcca ggaacacctc aattgattgc accaagctgt
     8281 gtgggtcctt attatgcaca aagtgcacca agacctgcaa caattgaaga attacacaca
     8341 atcgttgatc aatttggtca agcagctcac cgtgttcaga ttgccggtgg tgatggtgtt
     8401 gaaatccaat gtgcgcatgc ccacggatta ttaggtggtt tcttaacacc tttatataat
     8461 aaacgtacag atgaatatgg tggaaatatt gatggacgct tacgtttatg cttagaagta
     8521 atcgcatctg ttcgtaaata ctgtggagat gactttatta ttgatgttcg tattagtggt
     8581 gatgaatata gtgaaggtgg attgacatta aatgatcaaa tctatgtttg taagcaacta
     8641 gaaaaagctg gagttgactt tattcacgta tctggtggta acacaatcaa acgtggatca
     8701 agtatgcctg ctccaggtac aagtccagca cctcacgctc attcaagtga agaaatcaaa
     8761 aagcatgtaa atattcctgt tgccactgta gcacgtatta atgaaccttg gatggctgaa
     8821 gaactaatcg caaacgaaaa atgtgatatg tgtatgattg gtcgtccaaa cttatgtgat
     8881 agtgaatttg cgaataaggc atttgaagga aaagtagatg acattcgtcc atgtattgga
     8941 tgtggtcgtt gtttaacagg tatcatgatg ggtaaacgta ttggatgtac tgtaaatcca
     9001 agtgtggaag atgatacaat tgaacctgct gaaactaaga aaaaagtatt agttattggt
     9061 ggtggtcctg ctggtatgga agccgcatat gttgccaaaa aacgtggaca cgaagttgtt
     9121 ctttgtgaaa aatcgaatga acttggtgga caattaagac ttgcggcggt tccaattgcg
     9181 aaacaagaat tgtgtaaagt tattaaattt atgattcgtc gtttagatca tgaaggtgtt
     9241 gaagttcgtt taaatacaga agtcacaaaa gaaatgcttg aaaatgaatt taaagattac
     9301 gaaatcgtat gttcaagtgg agcaactcca aaagaaattc ctccattcaa agtatttaaa
     9361 aattggatga ctgcagacga tgtattatct ggaaagaaat atccaggacg taatattgtt
     9421 gtattaggtg gtggaagtgt tggatgtgaa acagccgatt atttagcacc attaatcaat
     9481 gacttattcc caatgaatcg taagattaca ttattagaaa tgacaaataa cttaatgcct
     9541 ggtgaaggtg gagctgcaaa atctagattg acacaaagat tgatgcagaa aggtgttcgc
     9601 atcgaattga atgcacaagt aacaagtgtg gatgaaaata caattacgta tgtaaaagag
     9661 ggtgttgaac acaagcttac agaagcggat actttaattt tcgcagtcgg ttatgcacct
     9721 aagaaagtgg aagtggaagc tgaaaatgta cactacattg gggattgtga aaaagtagga
     9781 actttaaaag atgcgatcac aaatgcgcat gagattgcaa aaaatatata ggagaaaata
     9841 tgacaagtaa attactacaa ccaattcaag ttggtaactt aacttttaaa aatagaatta
     9901 tgtttccccc tttgacaaca ggatatgaag aaagagatgg atcaataggt cctcgttcat
     9961 tagctttcta tacaagactt gcccaaggtg gatgttccta tattgttatt ggtgatgtgg
    10021 ctcctgttcg tacggcaagt cctacaccaa aactatatga tgaatcacaa attgaaatgt
    10081 ataaaaaatt ggcagatgcc ttacatgaat acgattgtaa agtagcttta caattgttcc
    10141 acccagaata tgatgttcaa ggcgttggaa aaatgatcat ggaagctggc attgctggtc
    10201 aattgtctgc aaaagcgaag gctgccaatg atgtagaaga ggctgaaaaa caacaaaaaa
    10261 tctgtgacga attgacaaag ggtgcatacg caaaattaca tcatgatatg cagcactttg
    10321 tgactgaagc aagtgttgaa caattaacag ctattaaaaa tagcattgcc caatgtgcta
    10381 gaaaagcaca aaaagctggt attgatgcga ttgaaattca tggggaccgt ttacttggtt
    10441 cattatgttc aactttatta aatcatcgta cagataatta tggtggtagc ttagaaaatc
    10501 gtactcgtta cgcattagaa gttttacaag cgattaaaga agcggctcca tccatgatgg
    10561 tcgaatataa acttccaatc attactgtca atccagatgg atctttacgt ggtaaaggtg
    10621 gtttattgga agatgaagcg gttgagttcg caaaaatgtt agatgcagca ggtattgata
    10681 tgattcaagt ggctcaagct aaccatacag gaaacatggg cgatacaatt cctccaatgg
    10741 gtgcggttcc ttataactgg actttaggtg cttgtgaaaa agtaaaagcc gttgtttctt
    10801 gtcctgtggc aacagtgggt cgtgttgtca gtgttgaagc tggtgaaaag attctagaag
    10861 atggtacagc tgacatgatt ggctatggaa gaagtttatt aacagatcca gatatcgcaa
    10921 ataaagtaga ctcaggtgaa tgcattcgtg aatgcttgaa ctgtaataaa ggttgtgtag
    10981 atgccatcca atctcgtcgt tatttaagtt gtgtattaaa tgccgaaaat ggagatgaag
    11041 aaacaatctt cattaaacca tgtacagaaa ctaagaatgt tgccgtagtt ggtgcaggtc
    11101 ttgcgggatt agaagcagct cgtgtagcat ataaacgtgg acatacagtg acagtgtttg
    11161 aaaaatcaga tcgtttagga ggacaaatca atattgcgtc agttcctcca agaaaagatg
    11221 aaattttacg ttcggttcaa tactacgaaa aattcttgcc tggtaaagtg gacattcaat
    11281 taaaccacga accaacaatg gaagaattga atggatttga ttatgtgatc ttggccattg
    11341 gagctcacaa tatggatctt cctatgcctg tagaaaattc aaatgtagta tccagctggg
    11401 atatcttgaa tggtcaagaa gtatctggta aatgtgtagt attaggcgga ggcttagtag
    11461 gtgctgaaac agctgaatat ttggcaaata aaggtctgga agtttcaatt gtagaaatga
    11521 tggataaaat tgcagcacaa gaatctgaaa ccgtattgcc attgatggaa gcggatttta
    11581 aagaacacaa tgttcaaaaa tttgtgaata cacgtgttag tgaaattaaa gataatgtaa
    11641 tctatgcaga aaacacaaaa gatgaaacaa atgttgaaat tacagcggat acaattgtaa
    11701 atgcattggg ttctaaaaag aatgtatttg atgatagtaa gttaagtgtt ccatttactt
    11761 atgtaggtga ctgcagtggt gaaagaacag cagatatcgc aagtgctatt cgtacaggat
    11821 ataaggctgc caatgaaatc taagagggtg tgaaaacacc ttcttttttt gtgcaaaaaa
    11881 tgaccatggt tcataaatat ataaatcata tataatatat gtctcaaaac ggtataaaaa
    11941 taatgaccat agctcataaa tatttacaca tttgaaaatt tgtctattaa aaatatattt
    12001 ttaaagaact aaaatgaaat tataaatgaa accggaggaa tcgttatgaa ttttagttct
    12061 ttacagagta gtgttcaaaa gtttggtatg aatcaagctt tgaagtattt agagaaggat
    12121 ccagaaacaa acattcctaa attaatgaat ttagtagata aggttatgcc tgaaggatgg
    12181 tatggttcac agcgtaaagt gattcgtgaa caaattgata aaaaaggtgt ttggtatgac
    12241 tacattttaa agttgtatga tttggatgca gatgttcggc agactgtgtt taaaaacttt
    12301 atttttaatg cgtctttaaa tggtagtaca aaacaagagg aattgtccga aaaatataat
    12361 tgtaatattc cttgggctgt attattagat ccaacatctg catgtaattt gcattgtaca
    12421 ggttgttggg ctgctgaata tggtcatcaa ttaaatttat ctttagacga tattgattcg
    12481 attatccgtc aaggaaaaga a
//
LOCUS       DBMDWL010000048        12483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1700_length_12483_cov_3.022048, whole genome shotgun sequence.
ACCESSION   DBMDWL010000048 DBMDWL010000000
VERSION     DBMDWL010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_12483_cov_3.022048"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            407..1303
                     /locus_tag="ACZPDN_04800"
     CDS             407..1303
                     /locus_tag="ACZPDN_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR1297971.1"
                     /translation="MNIILLVLGFILLLKGADWFVDGASSIASRFGIPQLIIGLTIVA
                     MGTSLPEAFVSITAALKSNAAITIGNVVGSNILNVGIILGITALIRPLHIQNSTIKYE
                     IPFMILVTLVLILLGINTTISRLDGIIMWLFFLGYLYYIFKMSKNQMEETEIKQTNPL
                     FIPLGLICLMMGSNFAVDAATNIAISLGVSQRFIGLTIVALGTSLPELVTSVTAAKKG
                     NADIAIGNIIGSNIFNILFVVGSSALITPVPFEPNFIIDSLVAILIGLVLYLCTKKTR
                     VLDKKAGILLLITYLFYFIYLL"
     gene            complement(1277..1447)
                     /locus_tag="ACZPDN_04805"
     CDS             complement(1277..1447)
                     /locus_tag="ACZPDN_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HIR1297972.1"
                     /translation="MDFLIVAACIFAMLKYLAHFKKTTNDQLDQKEPKKDDTVVLLEE
                     IRDLLKKIDKVK"
     gene            complement(1562..2773)
                     /locus_tag="ACZPDN_04810"
     CDS             complement(1562..2773)
                     /locus_tag="ACZPDN_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1297973.1"
                     /translation="MKKKGYNITLGIFIGLIAGVIVGIIMPEWANTALSVVASVYMSA
                     LKMMIFPLVFTSLVMGIIGIGDIAKTGKVGLHALLWYTGTTALASGLGLVLPRMLHLG
                     KNAHIVATKTQVEAATFNGLLDTLQNIIPSNPVEAFATGNMLQVLAFAVIIGLACLAI
                     KEKADPFVNLCDSIYQISIFIISKIMYVTPFGVFACIATVMYSNGVDTMLQLGQVLLA
                     LYITFYLYAFIVYGGMVKFFGKYSPIQFFKDIAPAGLNAFGTCSSSATIPISLKCASE
                     KIGIPEEITSLTLPLGATINMDAVSILMSFMITFFATALGIDLSIGTMVTVLACNVLL
                     SIGTPGVPGGAIAAFAALCPIAGIPTNQILGVYISVNTLCDMGATCVNVIGDLACSTV
                     LKQTLHFKKKK"
     gene            complement(2856..7169)
                     /locus_tag="ACZPDN_04815"
     CDS             complement(2856..7169)
                     /locus_tag="ACZPDN_04815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIR1297974.1"
                     /translation="MEVFEKLLDPELYAYFKNALFYEKPVFHTAHKMVEFYIEFENVI
                     PLEVYEQIITKLQLALHVHVKLHIHSKNNAVEMDELDRYVNHFVEKYSDVKDFRFLHP
                     YLENDTICFSTRDESRLAALNLALPSLVEKLKEVGINQEIVCKKVEENIAIETAKVQV
                     AAPVVKQEPVKKQYPSSNSYKKESATQYNIADLRVGYSNVQIEGHIFDIDNRVMKSGK
                     TLQMLYISDYDDAIVAKRFEGARCPIEEIQSVKKGMHVVLTGEVVYDSFSKCDSFMIR
                     KLEITQEKKREDHAKVKRTEWHVHSNFSEMDGVCAIEEFIQQAYDWGMDSIGVCDHQV
                     VQAFPMAQHKIEALVKQDPNREFKMLYGCEMLVADSKYHIVYNNDHRRLEDATFVVFD
                     LETTGLSNTRDEIIEFGGVKIKNREEVGRLQMFINPKRTIPAHISNLTHITQNDVDCA
                     KPIEDALDELLEFIGDSILVAHNATFDVGFMNAACRKTGRDELTNPVIDTLPLSYAML
                     PDEQKRFNLGSVCRHYRVSYDGEGAHRADYDADVLSQVMYNMMNQLGHEATLDTLMDM
                     EGGCEKYSSKKPYERNRPYHMTVFAKNKDGLKELFELITLSHTQYLVSMGKENGQPRI
                     IKEEISKKWDNGNLLIGSSCQNGEIFELAHTRSKKELFAAMSYYDYIELQPLDCYKNL
                     LDRGSITDVEDLKWYLQFIYDTAKEAGKMVIASSDAHYVNPNEKRLRDVYINAKAIGN
                     ARHPLYIYNKQARKATSNPNQHLLTTDEMLKAFEWMGEDVAYEVVVENTNKLKLLTEP
                     IFPIKDKLYPPDIEGSDQKLRDICFETAHQWYGKDLPDIVEKRLTRELDSIIGHGYYV
                     VYYISHLLVKKSNDDGYLVGSRGSVGSSFVATMSNITEVNPLTPHYVCKNCQHYEFLE
                     ADEAKSGFDLPDKVCPVCGEIMRGDGQDIPFETFLGFEGDKVPDIDLNFSGEYQPNAH
                     AYTKEVFGDDHVFRAGTVGTVQEKTAYGYVKGYEEEMECEPFNEAKRVDLAKGCEGVK
                     RTTGQHPGGIVVVPLDMDVHDFTPVQYPANNPNATWKTTHFDFHQIHDNILKFDILGH
                     VDPTAMKMLERITGVNVRQIPMNDQATMAIFSTTESLKIDTTKYNEVTGAAGIPEFGT
                     PFVRGILELTKPTTFAELVTISGLSHGTDVWLGNAKDLIDNGTCKLNEVIGCRDDIMV
                     DLMGYGVKPKLSFTIMESVRKGKGLKDEWVTEMKANNVPEWFIDSCTKIKYMFPKAHA
                     VAYVMMAVRIAWFKVHMPVHYYCMYFSIRCDAYDVQTMIQGEAAIRQRMADIKHMKED
                     KTQKPSDKELAIYDTLELALEMVLRGFRFSNISINRSAATEFIVDPDDPKAIIPPFTS
                     IDGLGSNVGLSVVKAREEKPFLSKEDVLKRTQLSKTLVEKLAEMNALEGLDEENQMTL
                     F"
     gene            complement(7171..7854)
                     /gene="rnc"
                     /locus_tag="ACZPDN_04820"
     CDS             complement(7171..7854)
                     /gene="rnc"
                     /locus_tag="ACZPDN_04820"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1297975.1"
                     /translation="MEKFIDWLKANGYKYNNEDIYLEACTHSSYANEHHRQMDDNERL
                     EFMGDSVLQIWSATQLFLMKPKMREGQMTTLRAQTVCESTLYVLNRQLGWYEFLRLGV
                     GEEKTGGRNRESILADQFEACIGAIYIDQGYHVCDEILTKYMKPVFKQEKSEAVTDYK
                     THLQEVIQADSRKTIQYKLLEEKGPSNAPTFTMGVYLDDILLGQGTSSTKKKAEQKAA
                     KDAFEKMAK"
     gene            complement(7856..8332)
                     /gene="coaD"
                     /locus_tag="ACZPDN_04825"
     CDS             complement(7856..8332)
                     /gene="coaD"
                     /locus_tag="ACZPDN_04825"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR1297976.1"
                     /translation="MKIAAFCGTFDPVTYGHLDIIERASKLFDELVVFISPNSDKNNE
                     FTEEIRLKWLNESTKHLANVTCKIQSGLVVEACKSVNATVLVRGIRNGVDCTYEQNMA
                     FMNARLDENIETVCLFTRPEYSLYSSSNVRELFKYGQDISGFVPACVLEDLKKGEE"
     gene            complement(8332..8883)
                     /gene="rsmD"
                     /locus_tag="ACZPDN_04830"
     CDS             complement(8332..8883)
                     /gene="rsmD"
                     /locus_tag="ACZPDN_04830"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR1297977.1"
                     /translation="MRIVAGKYGSRSLKSPKNDATRPTQDKIKGAIFSSLGNIFDGGN
                     FLDCYSGTGNMGLEAVSRGMDHATLVDNNKGAISVIKENVKNLKADKETKIICGNIFT
                     VLERLTLKYDVVYIDPPYAKQENEKLIQKLDDLDMVKLDGVIVVESLEEEVWPERIAS
                     FTKYKEKTYGITRISYYQKEENE"
     gene            complement(8880..10088)
                     /locus_tag="ACZPDN_04835"
     CDS             complement(8880..10088)
                     /locus_tag="ACZPDN_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1297978.1"
                     /translation="MSKQKRRSKLSFFALDPKCDKYINWAVALLLLAGTFTIISTNVG
                     KTTSAPNIVPKVLMKQCMFVLCGYIAMWFMNRWFRFKWYARFEKIIIPICLVIMATTF
                     AFPEIRGSRAWILIGGISLQPSEFAKPLIILICATCLYRAKKETDMLKVRGQLFKRAW
                     FSFVLIFGIVFAQKDMGTLVIICFIFLSCIMIPKYAVLQRMQRLLKLFVYGAIAAAIV
                     LFGITNIGTEIIKHTSLAHIAVRVENFKDPYIDVYGDGYQPANSLYGIASSDIRGKGI
                     GNSTRKYGYLTQADNDYILAVLLEETGVFGLFGLVILYGMIEIRLFYYAFKTSEDMYK
                     VILGGIGVYLFMHFFLNAGGVACLIPFTGVPLLFISSGGTSLMSIMLTMGLAQNCICS
                     IRRKEMDRLL"
     gene            10186..10737
                     /gene="def"
                     /locus_tag="ACZPDN_04840"
     CDS             10186..10737
                     /gene="def"
                     /locus_tag="ACZPDN_04840"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1297979.1"
                     /translation="MKITSKDIILDTDERIRMQSEPVSLPLSNEDKELLQAMLDYVRN
                     SQDDEIAKAEGLQPAVGIAAVQVGVLKQMIAVVIPYEDGVDEVALVNPKIISESVQNA
                     YLDNGEGCLSVKGEHPGHVFRHARIKVRGYDLIQDKNVTISAEGYFAICLQHEIDHLS
                     GTLFYDHIDANNPWKSDDEAEVI"
     gene            10812..>12483
                     /locus_tag="ACZPDN_04845"
     CDS             10812..>12483
                     /locus_tag="ACZPDN_04845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR1297980.1"
                     /translation="MPKTQEQTFGHASNKKPYHAKNHVEKGDTLIYALGGLGEVGKNM
                     YCYEHENEICIVDCGVLFPGDDLLGVDYVIPDYHHLIRMNKKRKFLVITHGHEDHIGG
                     IPFLLKQVQIDAIYAPRFAKALILKKLSEHTGLEKTKIIEIDENSSITTKYFKVGFFN
                     TIHSIPDSLGVHITCPNGSIVHTGDFKFDLTPVGTNADYQKMAFIGVTQPDLLMSDST
                     NSGVEDFSISERKVANEIQDIMRKTKQRLIVATFASNVHRVAQIIEAATKFGRKVIVF
                     GRSMENVVDIGRKMGTIKVKNSDFLSPEELAHTPDDKVCIICTGSQGEPLAALSRIAN
                     GTHRFIHLKPGDTIVFSSNPIPGNQSSVNRVIDNLFRSGATVLTKSILNNLHTTGHAS
                     KEEQKLMLQLVRPTYFMPIHGEYKMLMQHRQTAMETGIPKENIFVCANGDILILRDHK
                     VLQSDWRYQGDDIYVDGNDISGLSTAVLKDRRILADNGLVAVILAIDSKENKILMRPV
                     IVSRGFVFIKDSQGLIKEAEYIVNNSLQEKMKEKTTFSEIKNCVRSTLE"
BASE COUNT         4043 a         2539 c         1814 g         4087 t
ORIGIN      
        1 ttcgaatcat tcatgaatca agttctatac agttttcagg tttaagaact ctttctccat
       61 caaagaagta cgcattaaag aagtcgacaa aataggcatt gttttctaaa aactccttca
      121 taattttgtc tttggtttta ttcatgtgat ttactcaccc cctatatcta atacgaaata
      181 aattgaggtt tttctagaaa acttgcattt tttaagatat ttttacaatt aaatatagaa
      241 tttattaacc aaaatatgta taataagccc ataaagggag tagcctgcac atatatgtgt
      301 ttacagcttc gtcaatacga tattaaatcc ggactgtgat gaaatggcaa gactttggta
      361 cataagacca gagtcttttt attttatctg gtccaggagg aactatatga acataatttt
      421 attagtatta ggatttattc tactgctaaa aggagccgat tggtttgtcg atggagctag
      481 tagtatcgcc tcacgctttg gcatacccca actgattatt ggacttacta tcgttgcgat
      541 gggaacaagt ctaccagaag cttttgtttc tattacagcc gcattaaaat ccaatgctgc
      601 cattacgatt ggaaatgttg ttggaagtaa tattttaaat gtaggaatta ttcttggcat
      661 tacagcactc attcgtccat tacatattca aaatagtaca atcaaatatg aaattccatt
      721 tatgatactt gtaacacttg tattgatatt attaggtatc aacacaacaa tttctcgttt
      781 agatggaatt attatgtggt tattctttct tggttactta tactacattt ttaaaatgtc
      841 taagaatcaa atggaagaaa ctgaaatcaa acaaacaaat cctttattta ttccgctagg
      901 acttatatgt ttaatgatgg gaagtaactt tgcggtagat gcagccacaa acattgccat
      961 ttcattaggt gtcagtcaaa gatttatcgg cctaacaatc gttgccttag gaacttcatt
     1021 accagaactt gttacttctg taacagctgc caaaaaaggc aatgccgata tcgcgattgg
     1081 aaacatcatt ggatcgaata tctttaatat cttatttgtc gtaggttcta gtgcacttat
     1141 tactcccgta ccttttgaac caaactttat catcgattcc ttagtcgcaa tattgattgg
     1201 tcttgtcctt tatctttgta ctaaaaaaac aagagtactc gacaaaaaag ccggtatact
     1261 cttgttgatc acatatttat tttactttat ctatcttctt taacaaatcc cgaatctctt
     1321 ctaataagac aacagtgtca tcttttttag gttctttttg atcaagttga tcatttgttg
     1381 tctttttaaa atgcgctaaa tacttcagca ttgcaaagat gcatgccgca acaattaaaa
     1441 agtccacaac attttgaata aaattaccat acgtgattgt agctgtgcct acttgaattt
     1501 ttaagccgta aaatcaagtc caccaattaa aactccgatg attgttttat ttgtcttcat
     1561 cctatttttt ctttttaaaa tgtaaggtct gctttaatac agttgaacaa gccaagtcac
     1621 caatgacatt cacacaagta gctcccatgt cacacaaagt atttactgaa atataaacac
     1681 ctagaatttg atttgttgga attcctgcaa ttggacacaa tgccgcaaaa gcagcaatag
     1741 caccacctgg aacgcctgga gtaccaatac ttaacaatac gttacaagca aggacagtta
     1801 ccattgttcc aatacttaaa tcaatgccta aagctgtcgc aaagaatgta atcataaatg
     1861 acatcaaaat cgaaacagca tccatattga ttgttgcacc taaaggtaaa gttaaagacg
     1921 tgatttcttc aggaatacca atcttttctg aagcacactt taatgaaata ggaatcgtag
     1981 cactacttga acaagttcca aacgcattca atcctgctgg tgcaatatct ttaaaaaatt
     2041 gaattggact atattttcca aagaatttaa ccataccacc ataaacaata aatgcatata
     2101 aatagaacgt aatatataaa gccaataata cttgtcctaa ttgaagcatt gtatccacac
     2161 catttgaata cataactgtg gcaatacatg cgaatactcc aaatggagtc acatacataa
     2221 ttttactaat aataaaaata gaaatttgat aaatggaatc acacaaattc acaaatggat
     2281 ctgctttttc cttgatagcc aaacaagcta gtccaataat cacggcaaaa gctaatactt
     2341 gaagcatatt tcccgttgcg aatgcctcta ctggatttga aggaataata ttctgtaaag
     2401 tatccaacaa accattaaat gttgccgcct ccacttgtgt ttttgtcgca acaatatgtg
     2461 cattcttccc caaatgtaac atacgaggta aaaccaatcc tagacccgat gccaaagctg
     2521 ttgtacccgt ataccataat aaggcatgta aaccaacttt acctgttttg gcaatatccc
     2581 caatgccaat aattcccatt actaaacttg taaatacaag tgggaaaatc atcattttta
     2641 atgccgacat atatacggaa gcaacaacgc tcaatgcggt atttgcccat tccggcataa
     2701 taattccaac aataacacct gcaatcaagc caataaaaat accaagtgtt atattatatc
     2761 ctttcttttt cataactctc ctccaataat gtaaatattt taccatataa ctacatatat
     2821 gtaaaaattt acaaatacac taaaaaagac actgcctaaa acagtgtcat ttgattttct
     2881 tcatccaatc cctctaaagc attcatttca gctaactttt caactaaagt ttttgaaagt
     2941 tgtgtacgct ttaatacatc ttccttagaa aggaatggtt tttcttctct agcctttaca
     3001 acagataagc ctacgtttga acccaaacca tcaatacttg taaaaggtgg aataattgct
     3061 tttggatcat ctggatctac aataaattcg gtggctgcac tacgattaat actaatattc
     3121 gagaatctaa aaccacgcaa aaccatctcc aaagcaagtt ctaacgtatc ataaatagcc
     3181 aattctttat cacttggttt ttgtgtttta tcctctttca tatgtttgat atccgccatt
     3241 ctttgacgaa tggcagcctc cccttgaatc atggtctgca catcataagc atcgcaacga
     3301 atactaaaat acatacaata ataatgtact ggcatatgta ccttaaacca cgcaatacgc
     3361 acagccatca taacataggc aaccgcatgg gccttaggga acatgtactt gatttttgta
     3421 caagaatcaa taaaccattc cggtacattg ttcgccttca tttcagtaac ccattcatct
     3481 tttaagcctt tacccttacg gacactttcc ataatcgtaa atgataattt gggtttaaca
     3541 ccatacccca tcaaatcaac cataatatcg tcacgacaac caataacttc atttaactta
     3601 caagtgccat tatcaatcaa atcttttgca tttcccaacc aaacatccgt accatgagac
     3661 aaaccagaaa tagtgactaa ttccgcaaat gttgtcggct ttgtaagttc tagaatccca
     3721 cgaacaaatg gtgtaccaaa ctcaggaata ccagctgctc cagtcacttc attatatttt
     3781 gtcgtatcga ttttcagtga ttctgtcgta gaaaaaatag ccatagttgc ctgatcattc
     3841 attggaattt gacgaacatt gactcccgta attcgctcta acattttcat agccgtagga
     3901 tcgacatgcc ctaaaatatc aaattttaag atattatcat gaatctggtg gaaatcgaag
     3961 tgcgttgttt tccaagtcgc atttggattg tttgccggat attgaactgg tgtaaaatca
     4021 tgcacatcca tatccaaagg tacaacgaca ataccacctg ggtgctgacc cgttgttctt
     4081 tttacgcctt cacaaccttt ggccaaatct acacgctttg cctcattaaa aggctcacac
     4141 tccatctctt cctcatatcc tttgacatat ccatatgccg ttttttcctg taccgtacct
     4201 acagtaccgg ctctaaaaac gtgatcatct ccaaatactt cttttgtgta tgcatgggca
     4261 tttggctgat attcacccga gaagttcaaa tcaatatcgg gaactttatc cccctcaaaa
     4321 cctaagaatg tttcaaacgg aatatcctga ccatccccac gcataatttc tccacataca
     4381 ggacaaactt tatccggtaa atcaaagccc gatttggctt catccgcctc taaaaattca
     4441 taatgttgac aattcttaca tacatagtga ggtgttaatg gatttacttc tgtgatattt
     4501 gacattgtcg caacaaaact agaacctaca gatccacgcg aacctactag atatccatca
     4561 tcattgctct tttttacaag taagtgtgaa atataataaa ctacataata tccatgacca
     4621 atgatactat ccaattcgcg tgttaaacgt ttttcaacaa tgtctggcaa atcttttcca
     4681 taccattggt gagctgtttc aaaacaaata tcacgcaatt tttggtctga cccctcaata
     4741 tccggtggat acaatttatc cttaattgga aagataggtt ctgttaataa ctttaattta
     4801 tttgtgtttt ccacaacaac ctcataagct acatcttcac ccatccattc aaatgccttc
     4861 aacatttcat ccgttgtcaa caaatgctga tttggattgc tcgtagcctt acgcgcttgc
     4921 ttattataaa tatatagagg atgtctagca ttcccaattg ccttggcatt gatatacaca
     4981 tcacgaagac gtttctcatt tggatttaca taatgtgcat cacttgatgc gataaccatc
     5041 ttacctgctt cttttgcggt atcataaata aattgtagat accattttaa atcctccaca
     5101 tccgtaatac ttccacgatc caataaattc ttataacagt ccaatggctg aagttcaata
     5161 taatcataat agctcatggc tgcaaacaat tcttttttac ttcttgtatg agctagttca
     5221 aaaatctcac cattttgaca agaagaacca attaataagt ttccattatc ccacttcttt
     5281 gaaatttctt ccttaataat acgtggctgc ccattttctt tacccatact taccaaatat
     5341 tgtgtatgcg ataaagtaat caattcaaat aactctttta agccatcttt attcttggca
     5401 aaaacggtca tgtgatatgg acgatttctt tcataaggct tcttgcttga atacttttca
     5461 catccacctt ccatatccat caatgtatct aatgttgctt catggcccaa ttgattcatc
     5521 atattataca tgacttgtga aagtacatcc gcatcataat ccgcacggtg cgctccttca
     5581 ccatcataac tcacgcgata gtgacgacat acacttccta aattaaagcg tttttgctcg
     5641 tctggaagca tcgcatagga tagtggtaac gtatcaatca ccggattggt tagttcatca
     5701 cgacctgttt tacgacatgc tgcattcata aagccaacgt caaaggttgc gttgtgtgct
     5761 actaaaatgg aatccccaat aaattctaac aattcatcta aggcatcttc gattggtttt
     5821 gcgcaatcca catcattttg tgtaatatgc gttaaatttg aaatatgcgc aggaatcgtt
     5881 cgctttggat taatgaacat ctgtaatcgt ccgacctctt cacgattttt gattttcaca
     5941 ccaccaaatt caataatttc atcacgcgta ttcgaaagac ccgtagtttc caagtcaaat
     6001 acgacaaatg ttgcatcttc taatcttcga tgatcattat tgtagacaat atgatattta
     6061 gagtccgcca ctaacatttc acatccatac aacattttaa attcgcgatt tggatcttgt
     6121 ttgaccaaag cctcaatttt atgttgagcc atcggaaaag cctgaacaac ttgatggtca
     6181 cacacaccaa ttgaatccat accccaatca taggcttgtt gaataaattc ttcgatagca
     6241 caaactccat ccatttctga aaagttagag tgtacatgcc actctgttct tttcaccttg
     6301 gcatgatctt ctcttttctt ttcttgtgtg atttcaagtt ttcttatcat gaaagagtca
     6361 cacttagaaa aagaatcata tacgacttct cccgttaaaa cgacgtgcat tccttttttt
     6421 acagattgaa tttcttctat tggacatcta gccccttcaa aacgtttggc aacgatcgca
     6481 tcatcataat cactgatata tagcatctgt agtgttttac cacttttcat cacacgatta
     6541 tcaatatcga aaatgtgacc ttcaatttga acattcgaat agccaacacg taaatccgca
     6601 atattatatt gtgtagccga ttctttttta taactatttg aagaaggata ttgtttttta
     6661 accggttctt gttttacaac aggtgcagct acttgcacct ttgccgtttc aatcgcaata
     6721 ttttcctcta cttttttaca tacaatttct tgattgatac ccacttcttt taacttttct
     6781 accaagcttg gcaatgctaa atttaatgct gcaagccgag actcatctcg tgttgaaaaa
     6841 caaatcgtat cattttctaa atagggatga agaaagcgga aatccttcac atcactatat
     6901 ttttctacaa aatgattcac ataacgatct aattcatcca tttctaccgc gttatttttg
     6961 gaatgaatat gcaacttcac atgtacatgc agtgccagct gcaactttgt gatgatctgt
     7021 tcatatactt ctaaaggaat tacattctca aattctatat aaaactcaac cattttatgc
     7081 gctgtatgaa atacaggttt ctcataaaat aaggcatttt taaaatacgc atataattcc
     7141 ggatccaaca atttttcaaa tacttccatt ttatttagcc atcttttcaa aagcatcctt
     7201 agcggctttt tgttctgctt ttttcttcgt actacttgtt ccttgcccca ataaaatatc
     7261 atctaaatat acacccatag taaatgttgg agcattactt ggaccttttt cttctaataa
     7321 tttatattga atcgtcttcc tagagtctgc ctgaatcact tcttgtaaat gtgttttata
     7381 atctgtaaca gcctcacttt tttcttgttt aaatacaggc ttcatatact tagtcaagat
     7441 ttcatcacac acatgatatc cttgatcaat ataaatagct ccaatacaag cttcaaactg
     7501 gtcagccaaa atactttctc gatttcttcc acccgttttt tcttcgccaa cacctaaacg
     7561 taaaaattca taccatccta gttgtctatt taatacatat agcgtagatt cacatacagt
     7621 ttgtgctcga agtgttgtca tttgtccctc acgcattttt ggtttcatta gaaacagctg
     7681 ggttgcactc caaatctgta aaactgaatc tcccataaat tcaagtcgtt cattatcatc
     7741 catttggcga tgatgttcat tggcataaga agaatgtgta catgcctcta aataaatatc
     7801 ctcattgtta tatttatatc catttgcctt taaccaatcg ataaactttt ccattttatt
     7861 cttctccttt tttaagatct tctaatacac aagctggaac aaatccagaa atgtcttgtc
     7921 catatttaaa gagttctctt acatttgaac ttgaatataa actatattca ggtcgtgtaa
     7981 ataaacaaac tgtttcgata ttctcatcca atcttgcatt cataaacgcc atattttgct
     8041 cgtatgtaca atccacacca ttacgaattc cacgtaccag cacagtagca tttacacttt
     8101 tacaagcctc tactactaat ccagattgaa tcttacatgt tacgtttgcc aagtgtttcg
     8161 ttgattcatt caaccatttt aatcttattt cctcggtaaa ctcattgttc ttatctgagt
     8221 ttggtgaaat aaaaacaacc aactcatcaa acaatttgct tgccctttca ataatatcca
     8281 aatgcccata tgtcactgga tcaaacgttc cacaaaatgc tgctattttc attactcatt
     8341 ctcctctttt tgataataag aaatacgcgt aataccgtat gtcttttctt tatattttgt
     8401 aaaagaagcg atcctttcag gccacacctc ttcttcaagg ctttccacaa caataactcc
     8461 atccaatttt accatatcta aatcatctaa cttctgaatc aatttttcat tttcctgctt
     8521 tgcgtaagga ggatcaatat ataccacatc atattttaat gtgagccgct ctaacacagt
     8581 aaagatattt ccacaaataa tctttgtttc tttatccgcc tttaagtttt ttacattctc
     8641 cttgattacc gagatagcac ccttattatt atcgactaaa gtcgcatgat ccatacctcg
     8701 acttacagct tctaatccca tatttccagt tccactgtaa cagtctaaaa agttaccgcc
     8761 atcaaaaata tttcccaaac ttgaaaagat agcgcctttg attttatctt gtgtaggcct
     8821 agtcgcatca tttttaggac tctttaatga tctagagcca tattttcccg ccacaattct
     8881 cataataaac gatccatctc ctttcgtcta atcgaacaaa tacaattttg tgccaacccc
     8941 attgtcaaca taatcgacat taagcttgtt cctccactac taataaataa gagcggaact
     9001 cctgtaaaag gaattaaaca agcaacgcct ccagcattta agaaaaagtg cataaataga
     9061 taaacgccga ttccacctaa tattacttta tacatgtctt cacttgtttt aaatgcataa
     9121 taaaataaac gaatttcaat cattccatat aatataacta aaccaaataa tccaaaaaca
     9181 cccgtttcct caagcaatac agctagaata taatcattat ctgcctgtgt taaatagcca
     9241 tattttcgag ttgaattgcc tatacctttt ccacgaatat ctgaacttgc gataccatat
     9301 aatgaattgg caggctgata tccatctcca tatacatcga tatatggatc tttaaaattt
     9361 tcaacacgca ccgcaatatg agcaagcgat gtatgtttta taatttcagt accaatattt
     9421 gtgattccaa ataagacaat ggctgctgca attgcaccat aaacgaataa ttttaataat
     9481 cgttgcatac gttgtaaaac cgcatattta ggaatcataa tacaagatag gaaaataaaa
     9541 caaataatca ccaatgttcc catgtctttt tgagcaaata caattccaaa tattaaaaca
     9601 aatgagaacc atgccctttt aaaaagttgc cctcttactt ttaacatatc tgtttctttt
     9661 ttagctcgat ataaacacgt agcacaaata agaataatca atggttttgc gaattcactt
     9721 ggctgtaaag atatacctcc aatcaagatc catgcacggc ttcctctaat ttcaggaaac
     9781 gcaaatgtag ttgccataat aactaaacaa attggaataa ttattttttc aaatcttgca
     9841 taccatttaa agcgaaacca tctattcata aaccacatcg caatatatcc acataacaca
     9901 aacatgcatt gtttcatcag tactttagga acaatattcg gcgcagatgt tgttttccct
     9961 acatttgttg aaataatcgt gaatgttcca gccaataaca gaagcgcaac cgcccaattt
    10021 atatatttat cacattttgg atccaatgcg aaaaacgata attttgatcg ccgtttttgt
    10081 ttggacatga taatcccccc atcataaatt ctattttaac atattttcaa aaatcatttg
    10141 tcaataattc gaaaatgcta tagaatactt gcgaggatga ttactatgaa aataacaagc
    10201 aaagatatta tattagatac tgatgagcga attcgtatgc aaagtgagcc tgtttcattg
    10261 cctttatcca atgaagataa agaactttta caggctatgt tagattatgt ccgtaattca
    10321 caagatgatg aaattgccaa agctgaaggt ttacaaccag ctgttggtat tgcggccgtt
    10381 caagtcggtg ttttaaaaca aatgattgcg gttgtgattc cttatgaaga tggtgtagat
    10441 gaggtcgcat tagtcaaccc taaaatcatt tcagaatctg tacaaaatgc atacttagat
    10501 aatggagaag gctgtttatc tgtaaaaggt gaacatcctg gtcatgtttt ccgtcatgct
    10561 cgaattaaag ttcgtggtta tgatttgatt caagataaaa atgtaaccat ttcagccgaa
    10621 ggttatttcg caatttgcct tcaacacgaa attgatcact tgtcaggtac acttttctat
    10681 gatcacattg atgccaacaa tccttggaaa agtgatgatg aggcagaagt catttaagct
    10741 tctgcttttt taaattgact agctttgcta taataacaat agagtcatta aattatagaa
    10801 agaggtcgaa tatgccaaaa acacaagaac aaacgtttgg gcatgcatcg aacaaaaaac
    10861 catatcatgc aaaaaatcac gttgaaaaag gtgatacttt gatctatgcc ttagggggat
    10921 taggcgaagt tggtaaaaat atgtattgct acgaacacga aaatgaaatc tgtatcgtag
    10981 attgtggcgt tttgtttcca ggtgatgatt tattaggtgt tgactatgtt attcctgatt
    11041 atcaccattt gattcgcatg aacaaaaaaa gaaagttttt agtaattacc cacggtcacg
    11101 aagaccatat tggaggaatt cctttcttac ttaaacaagt ccagattgac gcaatttatg
    11161 cacctcgctt tgcgaaggct ttaattctta aaaaattaag tgaacataca ggactagaaa
    11221 aaacaaaaat tatagaaatc gatgaaaatt caagtattac tacaaaatac ttcaaagtag
    11281 gtttttttaa tacgatccac tcaattccag attctttagg tgtacatatc acatgtccta
    11341 atggatctat cgttcatacg ggtgacttta aatttgattt aacgcctgtt ggaacaaatg
    11401 ctgactatca aaagatggca tttattggtg ttacacaacc cgatttatta atgtctgatt
    11461 ccacaaactc tggagttgaa gacttctcta tttcagaaag aaaagttgct aatgaaattc
    11521 aagacattat gcgtaagact aagcaacgtt taatcgttgc aacattcgca tccaacgtac
    11581 atcgtgttgc ccaaattatc gaggcagcta ctaaatttgg aagaaaagtt atcgtctttg
    11641 gacgaagcat ggaaaacgtt gttgatattg gaagaaagat gggtacgatc aaagtcaaaa
    11701 attcagactt tttaagtccc gaagaacttg ctcatactcc agatgacaaa gtatgtatta
    11761 tttgtactgg aagtcagggg gaaccacttg cagctctatc aagaattgcc aatggtacac
    11821 accgttttat tcacttaaaa ccaggcgata caattgtctt ctcaagtaat ccaattcctg
    11881 gaaaccaatc cagtgtcaat cgtgttattg ataacttatt tagatctggt gctaccgtac
    11941 ttacaaaatc catcttgaat aacttgcata caacgggaca tgcttcaaaa gaagaacaaa
    12001 aactaatgtt acaattagtt cgtccaacgt actttatgcc tattcatggt gaatataaaa
    12061 tgttgatgca gcatagacaa accgctatgg aaactggtat tccaaaagaa aacatttttg
    12121 tatgtgcaaa tggtgacatt ctgattttaa gagatcacaa agtactacaa agtgactggc
    12181 gataccaagg tgatgatatc tatgtagatg gaaatgatat ttccggatta tctacagccg
    12241 tattaaaaga tcgtcgtatt ttagcagata atggtttagt tgccgttatt ttagctattg
    12301 attcaaaaga aaataaaatc ttaatgcgtc ctgttatcgt atcacgtgga tttgtcttta
    12361 ttaaagattc acaaggttta atcaaagaag ctgaatatat tgtaaataat tcacttcaag
    12421 aaaagatgaa agaaaaaaca actttctctg aaattaaaaa ctgtgtacgt tctactttag
    12481 aac
//
LOCUS       DBMDWL010000049        12356 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1735_length_12356_cov_2.264137, whole genome shotgun sequence.
ACCESSION   DBMDWL010000049 DBMDWL010000000
VERSION     DBMDWL010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12356)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12356
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1735_length_12356_cov_2.264137"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="ACZPDN_04850"
     CDS             <1..233
                     /locus_tag="ACZPDN_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297981.1"
                     /translation="GEYIKPTLSERFKTIEGHRVQRDLYSAFLICHTDDTLTAPNFKA
                     CHFDFPHFVKMQDTLILKMKKCGQTMKHCFGF"
     gene            578..916
                     /locus_tag="ACZPDN_04855"
     CDS             578..916
                     /locus_tag="ACZPDN_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297982.1"
                     /translation="MTKNTMIFIFLNMIFLLIWYATNKIRSTKVGKELDNGFEFYNSL
                     NTSDKENYWKEDTKILNLFFVLFIISMDISVILLFNENNLWIFSLVAGLIISSVVAII
                     LSINLKKKYK"
     gene            1061..1693
                     /locus_tag="ACZPDN_04860"
     CDS             1061..1693
                     /locus_tag="ACZPDN_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR1297983.1"
                     /translation="MAFDFKKEYKEFYMPKNKPVIVNVPEANYIAVRGEGNPNEEGGA
                     YQQAIGVLYAIAYTLRMSYKTDYKIEGFFEYVVPPLEGFWWQDDIDGVNYADKDSFHW
                     ISVIRLPDFVNEKDFEWAVETATKKKKIDCSSAEFLTIDEGLCVQIMHLGSFDDEPAT
                     VALMDEYLKQNGYANDMNSQRLHHEIYLSDARKVAPEKWKTVIRHPIRKI"
     gene            1723..2244
                     /locus_tag="ACZPDN_04865"
     CDS             1723..2244
                     /locus_tag="ACZPDN_04865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1297984.1"
                     /translation="MALKLMKGSLQYKNQIIEMIKEWKSYNDTHNANTSPWAIFKNDY
                     DDFEYYLNNLEISTTDDGFVPDSTFFCLDEDRDTMVGAVNIRHYLNEYLLKYGGHIGD
                     GIRPSERRKGYATKMISLALEECKKLGMDKVLLVCDKDNIGSVKSIINNGGVLENEVL
                     KEGKLIQRYWIML"
     gene            2427..3230
                     /locus_tag="ACZPDN_04870"
     CDS             2427..3230
                     /locus_tag="ACZPDN_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1297985.1"
                     /translation="MDSNVAENINILAKFLGTRDIDVLNQEELFERYGIHQADVMVLF
                     GGSIVEGGDVLASGIKNFVAKKYIIVGGAGHTTDTLRQVVHLEYPDIETTDLSEAEIF
                     QKYIKHVYGCKADYLETKSTNCGNNITYLLDLLKENNISFNSIILSQDASMQKRMAAG
                     LKKYVKDDVIIINYATYCAKVLNQNDELVYKENIHGMWTIERYVNLLMGEIPRLSDDA
                     NGYGPNGKNYIAHVDIPENVKIAFEELKMAFGSETREANPLYASKVIKK"
     gene            3384..5291
                     /gene="htpG"
                     /locus_tag="ACZPDN_04875"
     CDS             3384..5291
                     /gene="htpG"
                     /locus_tag="ACZPDN_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR1297986.1"
                     /translation="MAKKKQFKAESKRLLDLMINSIYTHKEIFLRELVSNASDAIDKY
                     YYESQGHADASQFEIRIEPNKEARTLTISDTGIGMSKEELEENLGTIAKSGSLAFKEE
                     MKKKEENNNEDVDIIGQFGVGFYSAFMVAKDVRVITKKAGSDEAYEWVSAGEDGYEIT
                     PCTKETNGTTIVLTLKDDTDEENYSKYLETYELKELIKKYSDYIRYPIKMNVETQKMK
                     EGTEESEKPEYETVVEDQTLNSMVPLWKRQKSKITDEEYNQFYKDHFYDYQDPQKVIH
                     FSVEGNTSFTALLYIPSHLPQGFYSQDYKKGLQLYCRGVFIMDHAEELLPDSLRFVKG
                     LVDSQDLSLNISREMLQHDHQLKLIAGRIEKKVLNELGNMLAKDREAYEKFFEEFGVN
                     LKFGVYNNYGMDKEKFQDLLLFYSSREKKYVTLSEYVSRMKEDQKDIYFVSGKDVELI
                     DKMPVVQTLKNKEFEVLYLTDEVDEFVMQTLMNYKEKTFRNAAQGDLDLDSEEEKEAL
                     NKSKEENKDLLTFMKEALGDEVAEVKLSNKLTEDPVCLTAGEGISFEMEKVFANMPNQ
                     SPMPMKATRILEINPNHPIFETLKTLYTSDKDKVKEVAEVLYDQACLIEGFAIKDPIA
                     YSKKICELLVK"
     gene            5360..7468
                     /locus_tag="ACZPDN_04880"
     CDS             5360..7468
                     /locus_tag="ACZPDN_04880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR1297987.1"
                     /translation="MKYQELMKEVEGVGKLKLSEQENFYRDVLNESSNDLEVRVAATF
                     YLGMAYYYEGNFNKAKEIIEPIILQYQSMPFVRELISAFNLMGVMLYYDGANVSSRYY
                     YEKALQIAMEHDDVGHYCYEYNNISITYVKQEKYEEALDSILKAKEYMPCCVEDMGAY
                     VYLNLALIYNSLNQVDKALEAFNIGLDEHDGKHIILEDYLNCGIELFSKAGMDFELNR
                     CADELEKRIETMYAVEYIDACIALFDYYLEQKMFVQVKAILRYMDSYMAKHPDEIRLG
                     LMVESSRYKYGKVICDLDVIILALEKKNAYYERIFEEEQVRRCHDTERYFEMNRKLQL
                     ALENETKANQVKMRFLASMSHDIRTPINGIIGMLQVIQRCGDDRQRIKDAYDKMWISS
                     THLLSLVNDILDIRTLETDSVVLENKSFKLSQLLGRVQTIIDVQASNYKIELFMKTQI
                     EHNHLMGSPTHIEKVLLNLLSNAIKYNKPGGKVYLSVKEKVDCFEFVVEDTGIGMSEE
                     FVQNKLFKPFVQKNTRIDGAGLGMSIVQELVKKMNGSIEVKSKENEGILFIVCLPLEV
                     DKNPVKTQSLKPKTVSIENLSILVVEDNDINMEVIEFMLQSEGAIVYKARNGKEAVEQ
                     FGQIKNLDLILMDLMMPIMDGYEATLQIRKKDKKIPILAMSANAYMQDIQKCYQAGMN
                     GHIGKPLYLEDLKDRIIQIL"
     gene            complement(7493..7903)
                     /locus_tag="ACZPDN_04885"
     CDS             complement(7493..7903)
                     /locus_tag="ACZPDN_04885"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1297988.1"
                     /translation="MIKPIMKDEAFLAIKSAPATKMDTQVAQDLKDTLQAYSDQCVGM
                     AANMIGVSKNIIIFAIGPAQIVMYNPKITKKSRPYKVKEGCLSLNGERNTTRYENITV
                     EYQDESFKKCKQSYSGFVAQIIQHEIDHCNGIII"
     gene            7968..8561
                     /locus_tag="ACZPDN_04890"
     CDS             7968..8561
                     /locus_tag="ACZPDN_04890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1297989.1"
                     /translation="MLKNIVFDLGNVLVKFDSNELIYSFFNERQEEVKSFYFDSLWNE
                     YDQGLYSVEEMIEKGVKQFPELELSIKKLMYHWTEFVIPLKDNVAYIKDLKRLGYNVY
                     ILSNIPEDDTKYLRSLGVFDNIDGGVFSYEYKKIKPDPEIFHILLEKYNLKALECLFL
                     DDRKDNVVAASNLGFETIEVKDSSKVIDLIKEKISEV"
     gene            8551..9771
                     /gene="rlmD"
                     /locus_tag="ACZPDN_04895"
     CDS             8551..9771
                     /gene="rlmD"
                     /locus_tag="ACZPDN_04895"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1297990.1"
                     /translation="MKCKVEKLCGGCSLLKLKPSMQADKKKKDVEALVEKAHLKVRVG
                     DVHMAKNDTHYRNKVIVGFAKDKGKVYSGLYASHSHRVVKTENCIMQPKLVNEIINKI
                     TELVGSMKLELYNERTGTGLLRHVLIRWGHKTDEVMVVFVTSQKMFPSRKNMVRVLTS
                     EFPQIKTIVQNINPRKTSIVLQDEAIVLYGNGMIHDELCGLDIAFSHNSFYQIHSEQC
                     EVLYGLAKKMLDLKETDTVLDTYCGVGTIGLTMTDSCKKVMGVEVNPDAIENAKYNAK
                     QNKIKNIEFVAMDSTEFMRQARKYHNHYDAIILDPPRAGTTKDFIECATALNPRKILY
                     ISCDPRTQVRDLNQFRKQGYVTNKLELVDLFPYTDHIESVCVLEKKQFNLKPTKKAQR
                     QAQFRSSKMSKKKR"
     gene            9858..10310
                     /locus_tag="ACZPDN_04900"
     CDS             9858..10310
                     /locus_tag="ACZPDN_04900"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR1297991.1"
                     /translation="MLEIGIQAPDFELPDQDGVMHKLSDYKGKKVILYFYPKDNTAGC
                     TKQACGFSERYPQFLEKGAVILGLSKDTVASHKKFQEKYGLAFTLLADPERKVIEAYD
                     VWKEKKNYGKVSMGVVRTTYLIDESGIIVRANDKVKAANDAENMLEVV"
     gene            10377..11105
                     /locus_tag="ACZPDN_04905"
     CDS             10377..11105
                     /locus_tag="ACZPDN_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1297992.1"
                     /translation="MWILYATGSAFFAGITSILAKCGISKTDSDVATAIRTIVVLVFS
                     WILVLLTGTLSGITVISGKILLFLVLSGIATGASWLCYFKALQIGDVNKVVPIDKSST
                     ILTILLALIFFHEGLSLAKIGCVCMIGLGTMLMITRKEDVESNSSDKSWLIYAILSAV
                     FASLTSILGKIGISGIDSNLGTAIRTSVVLVMAWLMVFVKNKQSEIISIDIKELLFIV
                     LSGFATGASWLCFYRALLLKSERS"
     gene            11212..>12356
                     /locus_tag="ACZPDN_04910"
     CDS             11212..>12356
                     /locus_tag="ACZPDN_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1297993.1"
                     /translation="MYTVRFQLELSGSEKRFLSKSFFYVNQMHNQLVRYATNRLNALF
                     HDKEYMGARKAYGEAEYSKKKGSELSASEKKKKKELSNIMCIKQKEYDLTKTSLCKFV
                     SKEQKKYKNYINSHQAQAEAEAVYKGVEKILFEDGRHLHYRRYNSFDCIKQKCAATGV
                     RLSKWDTICFMKHYYKVRVPKNEYIQNIISSVDLKEDVVYSFLKRIEFNSGFKYYVVI
                     TLRGDAPKRITLSEDGGHRTGVDFGTSTIATVSNNEAHLEELAPNSVNYEKEIRHKQN
                     LVDASMRKHNPENYNKNGTVKKGKHKWKTTRRCRRLKRQVRVLYRKQFAYIKTSHHTF
                     INRVVQNTSEFILEPMNFKALQKKSKKTKRQDKAVSIKQKDGSSKMVR"
BASE COUNT         4391 a         1542 c         2512 g         3911 t
ORIGIN      
        1 caggtgaata catcaaaccc actttaagtg aaagatttaa aacgattgaa ggacatagag
       61 tacaaagaga tttgtattct gcttttctaa tttgtcatac tgacgacaca ttgactgccc
      121 ctaattttaa agcctgtcat tttgactttc cacactttgt caagatgcag gatacattga
      181 ttctaaaaat gaaaaaatgt ggacaaacaa tgaaacactg ctttggattc tagcataaag
      241 aatctctcat ctagttcagg tctaaggcgt aagccctgct ggatacagta tgagaataca
      301 gagtacggtc cacatggatt ggaaatgctc gaaaaacaag agctatgcaa agactatatg
      361 caaggtctag aaccatgctg aaagctttgc gatgctccat ttctcgcatt gtgagaaaga
      421 gaatctcgtc attttaatga tgagagtacg tcaagaaaaa ttaggaatga aaaaagcaaa
      481 tgatgtgatg gaatataacc acattgaatg gacagcgttt actgtagagt aattaacgta
      541 atgtaataga taaatcagaa tttgtggagg aacaaacatg acaaagaata ctatgatttt
      601 tattttttta aacatgatat tcctacttat ttggtatgca acaaacaaaa taagaagcac
      661 taaagttggt aaagaactag acaatgggtt tgaattttac aatagtttaa acactagtga
      721 taaagaaaat tattggaaag aagatactaa aattttaaat cttttctttg tacttttcat
      781 aatatctatg gatatttccg ttattcttct ttttaatgaa aataatcttt ggatattttc
      841 attagttgca ggattaatta tttcatcagt tgttgcaata atattgagta ttaatcttaa
      901 gaagaaatac aaataagcga ataaattata gtttatgatc gtatagatta agacaaagcg
      961 tatttataaa tgaaaggctg agagagcagt ctttttgtat agttttcata tttaatggta
     1021 tactttaaaa aatagataaa accaaattta aggagtaatt atggcttttg attttaagaa
     1081 agaatataaa gaattttata tgcctaaaaa taaacctgtg attgtaaatg ttcctgaagc
     1141 aaattacatt gcggtcagag gtgagggcaa tccaaatgaa gaaggtggag catatcagca
     1201 ggcaattggc gtattgtatg cgattgctta tactttgaga atgagttata agacggatta
     1261 taaaattgaa ggattctttg aatatgtagt tccaccatta gaaggattct ggtggcagga
     1321 tgatatagat ggtgtgaatt atgcagataa agattcattt cattggattt cagtcattcg
     1381 attgcctgat tttgtgaatg aaaaagattt tgaatgggct gttgaaacag caactaaaaa
     1441 gaaaaaaata gattgttcat cggcagagtt tttgacgatt gatgaaggtt tatgtgttca
     1501 aatcatgcac ttaggatctt ttgatgatga accagctaca gttgcactta tggatgaata
     1561 tttaaaacaa aatggatatg cgaatgatat gaatagtcaa agactacatc atgaaatata
     1621 tttgtcggat gcaagaaagg ttgcaccgga aaaatggaaa acagtcattc gacatcctat
     1681 aagaaagatt tagataaatt ggaatttgga gggaataaat tcatggcatt aaaattaatg
     1741 aaaggcagtt tgcaatacaa aaatcaaatt atagaaatga ttaaagaatg gaaatcgtat
     1801 aacgataccc ataacgcaaa tacttcgcca tgggctatat ttaaaaatga ttatgatgat
     1861 tttgaatatt atctaaataa tttagaaata tcaactacgg atgatgggtt tgttcctgat
     1921 tctactttct tttgcttaga tgaagaccgg gataccatgg ttggtgctgt taatatcaga
     1981 cattacttaa acgaatattt attgaaatat ggtggtcata ttggagatgg tataagacca
     2041 agcgaaagaa gaaaaggtta cgcaaccaaa atgatcagct tggctttaga agaatgcaag
     2101 aaacttggta tggataaagt tttattggtt tgtgacaaag acaacattgg ttctgtcaaa
     2161 tcaattatca ataatggcgg agtattagaa aacgaagttt taaaagaggg taagctaata
     2221 caaagatatt ggatcatgtt gtaaatgtaa attcctgttt gcatacttat ggctgatata
     2281 ttttaaaggc caaattcgca aagttcgttg taaaaagtgt agtagaacaa caaaagttcg
     2341 ctgcattttt tgtgattcaa ttgtgagatt tatctttgat aacttccagt ttgccattgt
     2401 agatagtttt gaaaggaaga gtttatatgg atagtaatgt tgcagaaaat ataaatatac
     2461 tcgcaaagtt tttgggaaca cgagatattg atgtgttaaa tcaagaagaa ttgtttgaac
     2521 gatatggaat tcatcaagcg gatgtcatgg ttctatttgg tggaagtatt gttgaaggtg
     2581 gcgatgtttt agctagtgga attaaaaact tcgttgcaaa aaaatatatt attgttggtg
     2641 gagcaggaca tacaacagat acactacgtc aggttgttca tttagaatat ccggatattg
     2701 agacaacgga tttgtcagaa gcagaaatat ttcaaaagta tattaaacat gtatatggat
     2761 gcaaggcaga ttatcttgaa acaaagtcta caaattgtgg gaataatata acgtatcttt
     2821 tggatttatt gaaagaaaac aatatttctt ttaatagtat cattctaagt caggatgctt
     2881 ctatgcaaaa gcgtatggcg gcaggtttaa aaaaatatgt caaggatgat gtgataatca
     2941 ttaattatgc cacatattgt gctaaagtat tgaaccagaa tgatgaatta gtttataaag
     3001 aaaatataca cggcatgtgg acaatagaaa gatatgtgaa tttgttgatg ggagaaattc
     3061 ctagactttc agatgatgcg aatggctatg gtccgaatgg aaagaattat attgcacatg
     3121 tggatattcc cgaaaatgtg aaaatagctt ttgaagaatt aaagatggct tttggaagtg
     3181 aaacaagaga agccaatcca ttgtatgcat caaaagtaat taaaaaatag ttaacagtat
     3241 tatttgaaaa atgggtaaag aagcgaagaa ttcggttagt cgaattcttt tttacgttta
     3301 gcacacaggt gttgacactg ctaaaaaggt gttgtatagt attagcaggc aaaatattag
     3361 agtgctaatg aaggagtggt attatggcta agaaaaaaca gtttaaagcg gagtcaaaac
     3421 gtttattgga tttgatgatc aattccattt atacacacaa ggaaatcttc ttacgtgaac
     3481 ttgtatcgaa tgcgagtgat gcgattgata aatattatta tgaaagtcaa ggtcatgcag
     3541 atgcaagcca atttgagatt cgtattgagc caaataagga agctcgtact ttaacaattt
     3601 cggacactgg tattggtatg tcaaaggaag agttagagga aaacttgggt acaattgcga
     3661 agagtggttc tttggctttt aaagaggaaa tgaagaaaaa agaagaaaac aacaatgaag
     3721 atgtcgatat cattggtcaa tttggcgttg gattctattc tgcatttatg gtcgcaaaag
     3781 atgttcgtgt tattacgaaa aaagcaggta gtgatgaagc ttatgaatgg gtaagtgctg
     3841 gtgaagatgg ttatgaaatc acaccttgta caaaggaaac aaatggtaca acaatcgttt
     3901 taactttaaa agatgataca gatgaagaaa actattctaa atatttggaa acttatgaat
     3961 taaaagaatt aattaaaaaa tattcggatt atattcgtta tccaatcaag atgaatgtag
     4021 aaactcagaa gatgaaagag ggtacggagg aaagtgaaaa gcctgaatat gaaacagtgg
     4081 ttgaagatca gactttaaac tcaatggttc ctttatggaa acgtcaaaaa tcgaagatta
     4141 cagatgaaga atacaatcaa ttctacaaag atcatttcta tgactaccaa gatcctcaaa
     4201 aagtgattca ttttagtgta gagggaaata caagctttac agccttgctt tatattccat
     4261 cacacttacc tcaaggattc tattctcaag actacaagaa agggcttcaa ctctattgta
     4321 gaggcgtatt cattatggat catgccgaag aattgttgcc agactcattg cgttttgtga
     4381 aaggtttagt ggattctcaa gatttatctt taaatatttc tcgtgaaatg cttcaacatg
     4441 atcatcaatt aaaattgatt gcgggtcgta ttgaaaagaa agtattgaat gaacttggaa
     4501 atatgcttgc caaagatcgt gaagcatatg aaaaattctt tgaagaattt ggtgtaaact
     4561 taaaatttgg tgtctacaac aactatggta tggataaaga aaaattccaa gatttattgt
     4621 tgttctactc ttcaagagaa aagaaatatg ttactttaag tgaatatgtt tctcgtatga
     4681 aagaagatca aaaagatatt tactttgtat ctggtaaaga tgttgaattg attgataaga
     4741 tgccagttgt tcaaacattg aagaataaag agtttgaagt tctttactta acagatgaag
     4801 tggatgaatt tgtaatgcag acattgatga actacaaaga aaagacgttc agaaatgcag
     4861 ctcaaggaga tttagattta gattctgagg aagaaaagga agctttaaat aaatcaaaag
     4921 aagaaaataa agatttattg acatttatga aagaagcttt aggcgatgag gttgctgaag
     4981 tgaaattgtc aaataaattg acagaggatc cagtatgctt aacagctggt gaaggtattt
     5041 cttttgagat ggaaaaggtt ttcgcaaata tgcctaacca aagtccaatg cctatgaagg
     5101 caacgcgtat tttggaaatt aatccaaatc atccaatctt tgaaacattg aagactttat
     5161 acacttctga taaagataaa gttaaagaag tggctgaagt attatatgat caggcttgtt
     5221 taattgaagg attcgcaatc aaagatccaa tcgcatattc aaagaagatc tgtgaattgt
     5281 tagtgaaata attctaggca caaactttcg agtttgtgct tttttttcat tcatgataca
     5341 tttaatctag gtgaagatta tgaagtatca agaattaatg aaagaagtag aaggtgttgg
     5401 aaaactaaag ttaagtgagc aagaaaactt ttatcgtgat gtattaaatg aaagttcaaa
     5461 tgatttagaa gttagggttg cggccacctt ttatttgggt atggcttatt attatgaagg
     5521 aaattttaat aaggcaaaag agattattga accgatcatt ttgcagtatc aaagtatgcc
     5581 atttgtaaga gaacttattt ctgcattcaa tttaatggga gttatgctct attatgatgg
     5641 ggcaaatgtt tcaagtcgtt attattatga aaaagcttta cagattgcca tggaacatga
     5701 tgatgttgga cattattgtt atgagtataa taatatttca attacgtacg taaagcagga
     5761 aaagtatgaa gaggctttag atagtatttt aaaagccaaa gaatacatgc cttgttgcgt
     5821 tgaagatatg ggtgcctatg tctatttgaa tttagctttg atttacaata gcctaaatca
     5881 agtggataag gctttagaag catttaatat tggtttagat gagcatgatg gcaaacatat
     5941 tattttagaa gattatttga attgtggtat tgaattattt agtaaagccg gtatggattt
     6001 tgaattaaat agatgtgctg atgaacttga aaaacgtatt gagactatgt atgcggtaga
     6061 atatatagat gcttgtattg ctttatttga ttattatttg gagcagaaga tgtttgttca
     6121 agtcaaagcc atcttacgat atatggattc atatatggct aaacatccgg atgagattcg
     6181 tttaggttta atggttgaat cttctcgata taaatatggc aaagttattt gtgatttgga
     6241 tgtcataatt ttggcattag agaaaaagaa tgcatattat gagaggattt ttgaggagga
     6301 acaagtccgt cgttgtcatg atacagagcg ctattttgaa atgaatcgaa agcttcaact
     6361 cgccttagaa aatgaaacaa aagccaatca agttaagatg cgtttcttgg caagtatgag
     6421 ccatgatatt cgtacaccaa tcaatgggat tatcggaatg cttcaagtga ttcaaagatg
     6481 tggcgatgat aggcaacgca tcaaagatgc ctatgataag atgtggatat caagtacgca
     6541 cttgttgtct ttagtaaatg acattttaga tatacgaaca ttagaaacgg attcggttgt
     6601 gttggaaaat aaatcgttta aattaagtca attattaggt agggttcaaa ctattattga
     6661 tgtacaggca tcaaattata agattgaatt gtttatgaag acgcaaatag aacataatca
     6721 tttgatgggc agtcctacac atattgagaa ggtcttgttg aatcttttaa gcaatgcgat
     6781 taaatataat aaaccaggtg gtaaagtgta tttgtctgtc aaagaaaaag tagattgttt
     6841 tgaatttgtt gtagaagata caggaatcgg aatgagtgaa gaattcgttc aaaataagtt
     6901 attcaaacct tttgttcaaa aaaatacacg tatagatggt gccggtttag gtatgagtat
     6961 tgtgcaagag cttgtaaaga agatgaatgg gtccattgaa gtaaagagta aagagaatga
     7021 gggaattttg tttattgttt gtcttccact agaagtggat aaaaatcctg tcaagactca
     7081 atcattaaaa ccaaagacag tgtccattga aaatctatct atacttgtcg tggaagataa
     7141 tgatatcaat atggaagtga ttgagtttat gcttcaaagt gaaggggcca ttgtgtataa
     7201 ggctagaaat ggtaaagagg cggtagaaca gtttggtcaa atcaagaatt tagatttgat
     7261 tctaatggat cttatgatgc ctattatgga tgggtatgag gccactttac aaattcgaaa
     7321 aaaagataaa aagattccaa ttcttgcgat gagtgccaat gcgtatatgc aagatataca
     7381 gaaatgttac caggctggta tgaatgggca tatcggtaaa cctttatatt tagaggatct
     7441 gaaagatcga attattcaaa ttctataaaa agccaggttt ttcctggctt tattaaatga
     7501 tgataccatt gcagtgatca atttcatgtt gaatgatttg agcaacaaaa ccagaatagg
     7561 attgtttgca ttttttaaaa gattcatctt gatattcaac agtaatattt tcataacgag
     7621 ttgtatttct ttcaccattt agtgataaac acccttcttt gactttgtaa ggtctagact
     7681 tcttcgtgat ttttggatta tacatcacaa tttgtgcagg cccaatcgca aagataataa
     7741 tatttttaga aacaccaatc atattggcag ccatgcctac acattgatca ctataagctt
     7801 gtaatgtatc ttttagatct tgagccactt gtgtatccat ttttgtagct ggagctgact
     7861 taatagctaa gaaagcttca tctttcatta ttggtttaat cataacatac ctcttttctt
     7921 caaaaatcat atttgtttta gatgtttttt tcaagtataa ttggcatatg ctgaaaaata
     7981 tagtatttga tttaggaaat gtattggtta aatttgattc aaatgaattg atttatagct
     8041 tttttaatga aagacaagag gaagtgaaat cattctattt tgattcttta tggaatgagt
     8101 atgatcaagg actttattca gtggaagaga tgattgaaaa gggagtaaaa caatttcctg
     8161 aattagagtt aagtatcaaa aagttgatgt atcattggac agaatttgtg attccactaa
     8221 aagataacgt tgcgtatatc aaggatttaa aacgattggg atacaatgta tatattcttt
     8281 caaatattcc agaagatgac acaaaatatt tgagaagtct tggtgtgttt gataatatag
     8341 atggcggtgt tttttcttat gaatataaga aaattaaacc agatccagaa atatttcata
     8401 tattgttaga aaaatataat cttaaggcat tggaatgttt atttttagat gatcgaaaag
     8461 acaatgtagt tgcagcaagt aatttgggat ttgaaacaat tgaagttaaa gattcaagta
     8521 aagtcattga cttaataaag gagaaaatta gtgaagtgta aagtagaaaa attatgcggt
     8581 ggatgctcac ttttaaaact gaagccgagt atgcaagcag ataaaaagaa aaaagatgtg
     8641 gaagccttag ttgaaaaagc acatttaaaa gttcgtgttg gtgatgtgca tatggctaaa
     8701 aacgatacgc attatcgtaa taaagtcatt gttggttttg cgaaagataa agggaaagta
     8761 tattcaggtt tatatgcttc gcattcgcat cgtgttgtaa agactgaaaa ttgtataatg
     8821 cagccaaaat tggtaaatga aattatcaat aagataacag aacttgttgg ttctatgaaa
     8881 ttagaattat ataacgaaag aacagggacg ggtttattgc gtcatgtatt gattcgttgg
     8941 ggacataaga cagatgaagt gatggttgta tttgtcacaa gtcaaaagat gtttccttca
     9001 agaaaaaata tggttcgtgt attaacgagc gaatttccac aaattaaaac aatcgtacaa
     9061 aatatcaatc ctagaaagac aagtattgtt ttacaggatg aagctattgt tttatatgga
     9121 aatggtatga ttcatgatga actttgtggt ttagacattg cgttctcgca caattcgttc
     9181 tatcagattc attctgaaca atgcgaagta ttgtatggct tagctaagaa aatgttagat
     9241 ttaaaagaaa cagatactgt actagataca tattgtggtg ttggtacaat tggtttaacg
     9301 atgacagatt cttgtaagaa agttatgggt gtagaagtga atccagatgc gattgaaaac
     9361 gcaaaatata atgcgaaaca aaataaaatc aagaatattg aatttgttgc gatggattca
     9421 acagagttta tgcgccaggc aagaaaatat cacaatcatt acgatgcaat cattttagat
     9481 ccacctcgtg caggtacaac taaggatttt attgagtgtg caaccgcttt aaatccaaga
     9541 aaaattttat atatttcttg tgatccaaga acacaggttc gtgatttaaa ccaatttaga
     9601 aaacaaggct atgtcacaaa taaactagaa ttagtggatt tattccctta tacagatcat
     9661 attgaaagtg tttgtgtatt agaaaagaaa caattcaacc taaagcctac aaaaaaggcg
     9721 caaagacaag ctcaatttag atcaagtaaa atgagtaaga aaaagcgtta ggaagcaagt
     9781 atgcttcctt ttgtgcatat gagtaggttt aaataattta ggatgatata atttgtttaa
     9841 cagaacggag aatacatatg ttagaaattg gaattcaagc acctgatttt gaattgccag
     9901 atcaagatgg tgtgatgcac aaattgagtg attataaagg taaaaaggtt atcttatatt
     9961 tttatccaaa ggataatacg gctgggtgta caaaacaggc ttgtggtttt tctgaaagat
    10021 acccacaatt cttagaaaaa ggagctgtca ttttagggct aagtaaagat acggtggctt
    10081 cgcataagaa gtttcaagaa aagtatggcc ttgcctttac gttgttggca gatccagaaa
    10141 gaaaagtgat tgaagcttac gatgtgtgga aagaaaagaa gaattatgga aaggtatcta
    10201 tgggtgtagt tcgaacaaca tatttaatag atgaatcagg aattatagtt cgtgctaatg
    10261 ataaagtaaa agcggccaat gatgctgaaa atatgttgga agtagtttag acgctgcttc
    10321 ctttttgtat ttgtattaaa ggattatgag gtataaaatt tcatttgggt gatactatgt
    10381 ggattttata tgcgacaggt tctgcctttt ttgcaggaat tacttccata ctcgcaaaat
    10441 gtggaatctc aaaaacagat tccgatgtgg caaccgccat acgaactatt gtggttttag
    10501 ttttctcatg gattcttgtt cttttaactg gaacattatc gggtattacg gttatcagtg
    10561 gaaaaatttt gttgtttcta gttttgtctg gaatcgcaac aggagctagc tggctgtgtt
    10621 atttcaaggc tttacaaatt ggggatgtca ataaggtggt tccgattgat aaatccagta
    10681 cgattttaac aatattactg gctttgattt tctttcatga aggcttgtct ttagctaaaa
    10741 ttggctgtgt atgcatgatc ggtttaggta caatgttgat gattactcga aaagaggatg
    10801 ttgaatctaa ttcttcagat aaaagctggt tgatttatgc gattttgtca gctgtatttg
    10861 cgagccttac atcaatttta ggtaagattg gtatttctgg aattgattct aatttaggta
    10921 cggccattcg tacaagtgtt gtattagtca tggcctggct tatggtattt gtgaaaaata
    10981 agcagtctga aataatatct attgatatta aagaattatt gtttattgtc ttatctggct
    11041 ttgcgacagg agcatcttgg ctatgttttt atcgtgcttt actgttaaag tcagaacgct
    11101 cgtgacttta gtcatgagat gaatgacgta tgtaacagtt tttcaacagt ttctatacgg
    11161 gaaacatgtt ataatatata cgtagaatgc attatatgag gagatttcga tatgtataca
    11221 gtacgttttc aattggaact aagtggatct gaaaaacgtt ttttgtcgaa gtcttttttc
    11281 tatgtgaatc agatgcataa tcagttggtt cgatatgcta ctaatcgttt gaatgcttta
    11341 tttcatgata aagaatatat gggtgcacgc aaagcttatg gagaagctga atattctaaa
    11401 aagaaaggtt ctgaactatc agcttcagaa aagaagaaga aaaaagaatt gtcaaatatc
    11461 atgtgtatta aacaaaaaga gtatgatctt acgaagactt ctttgtgtaa gtttgtatcc
    11521 aaagaacaaa agaaatacaa gaactacatc aactctcatc aggcacaggc agaagctgaa
    11581 gctgtatata agggagtaga aaagattctt tttgaagatg gacgtcatct tcattatcga
    11641 agatataata gtttcgattg tattaaacaa aaatgtgctg caacaggagt gcgtctttca
    11701 aaatgggata ccatatgttt tatgaaacat tactacaagg ttcgtgtccc aaagaatgaa
    11761 tatattcaga acattatttc atctgttgat ttaaaagaag atgttgttta ttcattttta
    11821 aagaggatag aattcaacag tggattcaaa tattatgtag tgatcacact tcgaggtgat
    11881 gcaccaaaac gaatcacgtt gtcagaagat ggcggacatc gtacaggtgt ggacttcgga
    11941 acatcaacga ttgcgacagt ctcaaacaac gaggcccatc ttgaagaatt ggcaccaaat
    12001 tctgtaaatt atgaaaaaga gatccgccat aagcagaatc tggtggatgc ctctatgcga
    12061 aaacataatc cagaaaacta caacaagaat ggtacagtca aaaagggaaa acataaatgg
    12121 aaaactacaa gacgatgtag acgtttaaaa cgtcaggttc gagttcttta tcgaaaacaa
    12181 tttgcttata tcaagacttc acatcatacc tttatcaata gagtcgttca aaatacaagc
    12241 gaattcatcc ttgagccaat gaacttcaaa gctctgcaaa agaaatcaaa gaagactaaa
    12301 cgacaggata aagccgtatc cattaagcaa aaagacggct cttcaaagat ggtacg
//
LOCUS       DBMDWL010000050        12276 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1749_length_12276_cov_2.979713, whole genome shotgun sequence.
ACCESSION   DBMDWL010000050 DBMDWL010000000
VERSION     DBMDWL010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12276)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12276)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12276
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1749_length_12276_cov_2.979713"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="ACZPDN_04915"
     CDS             <1..453
                     /locus_tag="ACZPDN_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1297994.1"
                     /translation="LEKVYDCLLNETNEVFVDEEVDAVEEHAQDTLFEKENTVEEKEV
                     DEMEELARIAKQNRKEKTAADVKEYTSKFESMADASNKQEDVVSKPKKEKVEEEKKVV
                     EVKKFNYDDMKPIYSEEELAEIEEQEYEDENNWDDDVDYEEYDEYYDD"
     gene            465..725
                     /gene="rnpM"
                     /locus_tag="ACZPDN_04920"
                     /gene_synonym="ylxR"
     CDS             465..725
                     /gene="rnpM"
                     /locus_tag="ACZPDN_04920"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR1297995.1"
                     /translation="MRKIPMRKCVVTQERFPKKDLIRVVRTPEKTVEIDLTGKKNGHG
                     AYISKSLDTLALAKKNKALQRALEIEIPEEVYQKLESIITNE"
     gene            718..1008
                     /locus_tag="ACZPDN_04925"
     CDS             718..1008
                     /locus_tag="ACZPDN_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013417.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HIR1297996.1"
                     /translation="MNKVTNLIQLAYRAGKVAKGDSLLVSIQKSQAKLVLVSSLCGQN
                     RMKKIQDKCTYYNVPYRVVEPSVLDDVSYKKMSAIAIVDNGFAKAIIEKMKG"
     gene            1017..2864
                     /gene="infB"
                     /locus_tag="ACZPDN_04930"
     CDS             1017..2864
                     /gene="infB"
                     /locus_tag="ACZPDN_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1297997.1"
                     /translation="MAYNNKNKKKPNRKGHKSSYQSNAPKKEEAVKEITYSDAITVGQ
                     LAQLLHKNSSEIIKFLFMMGNMSTINTVLSDEDIELICMNFNVEVHKEIIVDEDDIEE
                     QLQNVEEDESKLVPRPPVVTIMGHVDHGKTTLLDTIRKANVTENEFGGITQHIGAYQV
                     SLKGRKVTFLDTPGHEAFTAMRARGAEVTDIVIIVVAADDGVMPQTKEAVDHAKAAGV
                     PIVVAVNKIDKPGANPDRIMSEMSELGIMPEEWGGDTIFTQVSAKKGTGVPELLETML
                     LVADMQELKANPDTNASGTVIEAKLDKGRGPVATLLVQRGTLHTGDSVVVGTSYGRVR
                     KMTNDRGMEIKHAEPSCPVEIIGLNEVPRAGDVFMSYDSYKKAAEIAGHRLDKQIEKE
                     RNSTSAMSLEDLAKRIDEGDVQEINVLIKADVQGSAEALKASMEKLEVDGNVRINVIR
                     STVGTITESDILLASASNAIIYGFNIRPSAAIRKKAEEEGVEIRLHNIIYKALEELQA
                     AMKGMLAPVYEEVVIGQADVRQIYKISKVGTIAGCMVTDGKMKRDCKVRLIREGIVVY
                     TGKLGSLRRFENDVKEVLNGYECGMTIENFNDIKVGDILEGFEDQEVEE"
     gene            2866..3204
                     /gene="rbfA"
                     /locus_tag="ACZPDN_04935"
     CDS             2866..3204
                     /gene="rbfA"
                     /locus_tag="ACZPDN_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR1297998.1"
                     /translation="MSLKTERMEMTIQREISRILQFELKNPNLGFCTITDVRCTNDLS
                     QAKVYVTFLGKQGRNDAGMRVLNQSKGFIRSQVAKKIKIRKMPELIFVQDTSLEQGNK
                     IENILKEIEK"
     gene            complement(3484..4668)
                     /locus_tag="ACZPDN_04940"
     CDS             complement(3484..4668)
                     /locus_tag="ACZPDN_04940"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR1297999.1"
                     /translation="MTKKVVLAGACRTAIGKMGGALSNTPAADLGSIVIKEALNRAGV
                     KPEQVDEVLMGCVIQAAQGQNVARQASIKAGLPIEVPAVTLNVVCGSGLNCVNQAAAM
                     IMAGQADIVVAGGMENMSMAPYAMTKARFGYRMNNATIVDTMVNDALTDAFNHYHMMI
                     TAENICDRWNLTREELDEFSANSQQKAEKAMAEHKFDDEIVPVPVKVKKQIVEFKVDE
                     GPRPGTTVETLAKLRCCSGKEGGMVTAGNASGINDGAAAIVVMSEEKAKELGVTPMAT
                     WVQGALAGVEPEIMGIGPVAATRKVMERTGLTVDDMDLIEANEAFAAQSVAVAKELGF
                     DMSKVNVNGGAVALGHPVGASGCRILVTLLHEMQKRDAKKGLATLCIGGGMGCATIVE
                     RD"
     gene            complement(4804..6009)
                     /locus_tag="ACZPDN_04945"
     CDS             complement(4804..6009)
                     /locus_tag="ACZPDN_04945"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR1298000.1"
                     /translation="MRKAYIVAAKRSAIGTFLGSLTDVDLATVGATVLKETIKQANID
                     PANLDEVIIGNVIAAGLGQNIARHVAFDAGIPVEVCAQSINMLCGSGLKAVMEATLRI
                     QANFGDLYVAGGVESMTRAPFMLSYKNRTGTKMGDQKLVDAMLHDGLTDAMYGIHMGC
                     TADNIAKKYNISREAQDEFAFASQEKALAAIAAGKFKDEIVPITYKTRKGEVVFDTDE
                     HPRKTSLEKLAKLRPAFNADGTVTAGNASGINDGASFTIVASEDAVKKYNLTPIAEVI
                     GFGQGGVDPRVMGLGPTPAILNALKYADLKIEDLDLVELNEAFAAQSLGVIHELEEAT
                     GMDREAFLAKTNVNGGAIALGHPVGASGNRILVSLLYEMQKRNSKIGLASLCIGGGQG
                     AAVIVKMCD"
     gene            6299..7438
                     /locus_tag="ACZPDN_04950"
     CDS             6299..7438
                     /locus_tag="ACZPDN_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR1298001.1"
                     /translation="MDFNLTEQQLMMQKLFREVAQNEVKENAADVDENERFPKESVEV
                     MRQAKMLGIPYSREYGGAGADYLCYILAIEELSKTCATSGVVLSAHTSLGTWPIAQFG
                     TEEQKQRFLTDLCTGKKLAAFGLTEPNAGTDAAGQQTTAVLDGDEYVINGTKIFITNA
                     GEADVYVIFAMTDKTKGTRGISAFILEKGTPGFTFGLHEKKLGIRGSATCELIFNNVR
                     IPKENLLGKEGMGFKIAMQTLDGGRIGIAAQALGIAQGAIDEAVAYVKQRKQFGRPIA
                     KFQNTQFQLADMQTKTDAARWLVYSAATAKQEGRPYTQLAAEAKLFAAETAMEITTKA
                     IQLLGGYGYTRDLPVERMFRDAKITEIYEGTSEVQRMVISGAMLK"
     gene            7460..8254
                     /locus_tag="ACZPDN_04955"
     CDS             7460..8254
                     /locus_tag="ACZPDN_04955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR1298002.1"
                     /translation="MKIVVLEKQVPDSTEITVDKKTGALIRDGVPAITNPDDLAGLEA
                     ALELKDKNPDTEVVVMTMGLPKAAEMLKEGVAMGADKGVLLTDRVLGGSDTCATSIAL
                     AAALKKVGFDLVIAGRQAIDGDTAQVGPQTAERLDIPQVTYVDEILEVKEDSITVKRS
                     LEDVEQIVTAKLPCVITTLSGMNKPRYMQCDNIVDLCKEDKIEVLTNAELGIDPETVG
                     FKGSPTSVKTTFTKDVTDKTEVFTLSEQETADMIAKTLKEKQIITK"
     gene            8270..9301
                     /locus_tag="ACZPDN_04960"
     CDS             8270..9301
                     /locus_tag="ACZPDN_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR1298003.1"
                     /translation="MARFEEYKDIFVFVEQENGNIAEVSYELISEARKLVASIPEFGY
                     KVVGVLLGENVKEKAQEVISHGADKVIVVDDALLKDYSTQFYADALTQVVLAHKPDSM
                     LTGATPLGRDLAPRVAARLNVGLTADATKIEVDLEYAKETGESLLDVTRPTFGGNLFG
                     TIVCKDTRPQMATIRPKVFELAPTDTTRTGEVEEFTPSWTTTDPQVFVEKVIEKVKAD
                     VDITKANILVGAGRGAENCLDVLQKVADELGGVVCCTRAVVEDGFLDKDRQVGQTGKT
                     VRPSLYIACGISGAVQHCAGMDKSDLIISINRDPQAEIFNISNMGFVGDCATVLPLLV
                     ESIKAAKAE"
     gene            9322..10158
                     /locus_tag="ACZPDN_04965"
     CDS             9322..10158
                     /locus_tag="ACZPDN_04965"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR1298004.1"
                     /translation="MKNVGVIGAGTMGQGIANAFATAGYNVTVCDIKIEWAQNGINKI
                     GAKLDKLVSREKITAEKAEGIKANLTAGEYKDLADCDLIVEAVLEKMEIKKDLFKTLD
                     EICKEDCIFGTNTSSLSVTEIANGIKHNVIGMHFFNPADRMKLVEVISGENTPVETKE
                     AIIECSKSLGKTPVEVAEGPGFVVNRILIPMINEACFIYQEGLASVEDIDTAMKLGAN
                     HPMGPLALGDLIGLDIVLDVMEVLYTETGDSKYRPCTLLKKMVRAGKLGQKTKQGFYS
                     YN"
     gene            10223..11002
                     /locus_tag="ACZPDN_04970"
     CDS             10223..11002
                     /locus_tag="ACZPDN_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR1298005.1"
                     /translation="MENVLLEKQGHVSIITINREKALNALSTAVLNDLEEAVNTVSAD
                     KDTYCVVITGAGSKSFVAGADIAEMKDKTVEEAAEYGAYGNKIFRQIETLHCPVIAAV
                     NGFALGGGCELSMACDIRIASENAIFGQPEVGLGITPGFGGTQRLARLVPAGIAKEMI
                     FTARNIKADKALAIGLVNAVVPQEELMATALKMANGICKNAPIAVAQSKKAINAGLQT
                     DMDSAIAIEVKDFSDCFATEDQTYGMECFVNKVKEKEFKNK"
     gene            11025..>12276
                     /locus_tag="ACZPDN_04975"
     CDS             11025..>12276
                     /locus_tag="ACZPDN_04975"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HIR1298006.1"
                     /translation="MSKVMSAQEAISLIKDGDKIGVGGFIGMGHPQELSVAIENSFLE
                     TGHPRDLTVMFSAGVGDGTPDLGLNHMGHEGLLKRIIGGHWGLIPTFQKLVFENKVEG
                     YNLPLGTISLMFREIAGHRPGVITKIGLKTFIDPRLEGAKMNERTKEDLVELINMDGQ
                     EWLRYKSFPLDIALIRGTYADEDGNCSMCKEAATLDSISIAQAVKNSGGKVILQVEKI
                     VERGSLKPMDVKIPGIYVDAIVVAQPENHWQTYSDPYNPSFCGEIRVPVDSIEPMPLN
                     ARKVVCRRAAMELDPSAVINLGIGMPEGIANVANEEGLPGLKMTVEAGGIGGVPMNGT
                     AFGACTNPDAIIDQTYQFDFYDGGGLDQAFLGLAECDQKGDINVSRFGPKIAGCGGFI
                     NITQTSPVVVYCGTFTAKGLKETVG"
BASE COUNT         4169 a         1989 c         2484 g         3634 t
ORIGIN      
        1 ttagaaaagg tttatgattg tttgttgaat gaaacaaacg aagtgtttgt agatgaagaa
       61 gtggatgcag ttgaagaaca tgcacaagat actttatttg aaaaggaaaa tacagttgaa
      121 gaaaaagaag tcgatgaaat ggaagaattg gctcgtattg ccaaacaaaa tcgtaaagaa
      181 aaaacggctg cagatgtaaa ggaatatact tctaaatttg aatctatggc cgatgcttca
      241 aataaacaag aggatgttgt ttctaaacct aaaaaagaaa aggttgagga agaaaagaaa
      301 gtcgttgaag ttaagaaatt caactatgat gatatgaagc caatctattc tgaagaagag
      361 ttagctgaaa tcgaagaaca ggaatatgaa gatgaaaaca actgggacga tgatgttgac
      421 tatgaagaat atgatgaata ttatgatgac taggagttag tttcatgcgt aagattccaa
      481 tgcgaaaatg tgtcgtaaca caagagcgtt ttcctaaaaa ggatttgatt cgtgtggtac
      541 gtacacctga aaaaacagtt gagatcgatt tgacaggtaa aaagaatggg catggtgctt
      601 atatttcaaa aagtttggat actttagctc ttgccaaaaa gaacaaggct ttacaaaggg
      661 ctttagaaat tgaaattcct gaagaagtat atcagaaatt agaatccatt attaccaatg
      721 aataaagtta caaatttgat acagctagca tatcgtgctg gtaaagtagc aaagggtgat
      781 tctttacttg ttagtattca aaaatctcaa gctaaactag tacttgtatc ttctttatgc
      841 gggcaaaacc gtatgaagaa gattcaggat aaatgcacat attataatgt gccatataga
      901 gtggtagaac catctgtatt agatgatgtt tcttataaaa agatgagtgc tattgcgatt
      961 gtagataatg gctttgccaa agcgattatc gaaaagatga aaggttaggt gattcgatgg
     1021 cttataacaa caaaaacaaa aagaaaccaa atcgaaaagg gcataagagt tcgtatcagt
     1081 ctaatgcacc taagaaggaa gaagcagtaa aggaaatcac atatagtgat gcgattactg
     1141 taggtcagtt agcacaattg cttcataaaa atagttctga aatcattaaa ttcttattta
     1201 tgatgggaaa tatgtcaaca atcaatactg tattatcgga tgaggatatt gaattgatct
     1261 gtatgaactt caatgtagaa gtgcataagg aaattattgt ggatgaagat gatatcgaag
     1321 aacaacttca aaatgtagaa gaagatgaat ctaaattagt acctcgtcct cctgttgtca
     1381 caatcatggg tcacgttgac catggtaaaa caacattatt ggatacaatt cgtaaagcga
     1441 atgttactga aaatgaattt ggtggtatta cacaacatat tggtgcttac caggtatcct
     1501 taaagggaag aaaagttaca ttcttagata ctccaggtca cgaagcattt actgctatgc
     1561 gtgctcgtgg agctgaagtt acagatatcg ttattattgt agttgcggca gatgatggtg
     1621 taatgcctca aacaaaagaa gcagttgacc atgccaaagc ggctggtgtt ccaattgtag
     1681 ttgcggtaaa taaaattgat aagccaggtg caaatccaga tcgtatcatg tctgaaatga
     1741 gcgaactagg aattatgcct gaagaatggg gtggagatac aatctttact caagtatctg
     1801 caaaaaaagg aacaggtgtt ccagaattat tggaaacgat gttgttagtg gccgatatgc
     1861 aggaattaaa ggcgaatcca gatacaaatg catcgggtac tgtaattgaa gcgaagttag
     1921 ataaaggtcg tggaccagta gctacattgc ttgtacaacg tggtacattg catactggag
     1981 atagtgttgt agttggtaca tcttatggtc gtgttcgtaa aatgacaaat gatcgtggta
     2041 tggaaatcaa acatgcagaa cctagttgtc cagttgaaat tattggttta aatgaggttc
     2101 ctagagctgg agatgtcttc atgtcttatg atagctataa gaaggcggct gaaattgcgg
     2161 gtcatcgttt ggataaacaa attgaaaaag aacgtaattc aacgagtgca atgtctttag
     2221 aagatttggc aaagagaatc gatgagggtg atgttcaaga aatcaatgtc ttgatcaaag
     2281 cagatgttca aggttcggct gaagctttaa aggcaagcat ggaaaaacta gaagtggatg
     2341 gaaatgttcg tatcaacgtt attcgttcta ctgtaggtac aattacagaa tcagatatct
     2401 tattagcaag tgcttcaaat gcgattattt atggatttaa cattcgtcca agtgctgcta
     2461 ttcgtaaaaa agcggaagaa gaaggtgttg aaattcgttt acacaacatc atctataagg
     2521 ctttagaaga attacaagct gcaatgaaag gtatgcttgc acccgtatat gaagaagttg
     2581 tcattggtca ggcggatgtt cgtcagattt ataagataag taaggtaggt acgattgccg
     2641 gatgtatggt tacagatggt aagatgaaac gtgattgtaa agttcgtttg atccgtgaag
     2701 gtattgttgt ttataccggt aaacttggat cattacgtcg ttttgaaaat gatgttaaag
     2761 aagtactaaa tggttacgaa tgtggtatga caatcgaaaa ctttaatgat attaaagtag
     2821 gagatatttt agaaggcttc gaagatcagg aagtggaaga atagtatgtc attaaaaaca
     2881 gaacgtatgg agatgacaat tcaaagagag atttcacgta ttcttcaatt tgaattgaaa
     2941 aatccaaatt taggtttttg tacgataacg gatgtacgat gtacaaatga tttatcacaa
     3001 gctaaagttt atgttacatt ccttggaaaa caagggcgta atgatgcggg tatgcgtgtg
     3061 ttaaatcaat caaagggttt cattcgttct caagtagcaa aaaaaataaa gattcgtaag
     3121 atgccagaat tgatctttgt acaagataca agtcttgaac agggaaataa gattgaaaac
     3181 attttaaagg aaatcgaaaa atagagaaga cttagtggtc ttctctttta ttgtttgtcg
     3241 atagtattaa gcaacagagt cctaatggta gataagtaaa attctttgtt aagaagtatg
     3301 tgaatataaa acatatccct gcgaaaataa ataatattcg atttaaattt gtgttcatag
     3361 ttatagtaac tcctttaaat tgattataac aaaaaaattg ttgaattgta aaaagtaaga
     3421 aaaaaagaag agattcagtt ttgttgaatc tctttttaga agtagttagt tattatatgt
     3481 aaattagtca cgttctacga tagtagcaca acccatacca ccaccgatgc aaagtgtagc
     3541 taaacctttc ttagcatcac gtttttgcat ttcgtgaagt aatgtaacta ggatacggca
     3601 tccactagct ccaacagggt gtcctaatgc aacggctcca ccattaacgt taactttgct
     3661 catatcgaat cctaattctt tagcaacagc tacagattgt gcagcgaatg cttcgtttgc
     3721 ttcgatcaag tccatatcat caactgttaa tccagttctt tccataactt tacgagtagc
     3781 agccactggt ccaataccca taatttctgg ttcaacacca gctaatgcac cttgtaccca
     3841 agtagccatt ggtgtaacac ctaattcttt tgctttttct tcgctcataa caacgattgc
     3901 agcagcacca tcattaattc ctgaagcatt accagcagtt accattccgc cttctttacc
     3961 agagcagcaa cgtaattttg ccaatgtttc aacagttgtt cctggacgag gtccttcatc
     4021 cactttgaat tcaacgattt gttttttaac tttaactgga actggtacga tttcatcatc
     4081 gaatttgtgt tcagccatag ctttttctgc tttttgttga gagttagctg agaattcatc
     4141 taattcttca cgagttaagt tccaacgatc acagatgttt tcagcagtaa tcatcatgtg
     4201 gtagtggttg aatgcatcag ttaatgcatc gtttaccatt gtatcaacga tagtagcgtt
     4261 gttcatacgg taaccaaaac gtgctttagt catagcatat ggtgccattg acatgttttc
     4321 cataccacca gcaacaacga tatcagcttg gccagccatg atcattgcag cagcttggtt
     4381 aacacagttt aaaccagatc cacaaacaac gtttaatgtt acggcaggaa cttcaatagg
     4441 tagaccagct ttgatactag cttgacgagc aacgttttgt ccttgggcag cttggataac
     4501 gcatcccatt aatacttcgt caacttgttc aggtttaaca cctgcacggt tcaatgcttc
     4561 tttaattacg atagaaccta aatcagcagc tggagtgtta cttaaagctc cacccatttt
     4621 accaatagca gtacgacatg cacctgctaa aactactttt tttgtcatat taatattcct
     4681 ccatgtaaga ctattagtca tttacctttt aaactttatc atagtgaatg atgaaaatca
     4741 atcacaaatt aaaaaattca caagtgaatt taaagaaata aggagaacat gtctccttat
     4801 tgattaatcg cacattttta cgataacagc ggcaccttgt cctccaccaa tacataggga
     4861 agcaagaccg atttttgaat tacgtttttg catttcatat aacaatgaaa ctaagatacg
     4921 gtttccacta gcacctactg ggtgacctaa agcaatagct ccaccattta cgtttgtctt
     4981 agctaagaat gcttcacgat ccatacctgt agcttcttct aattcgtgga taacacctaa
     5041 tgattgagca gcaaatgctt cgttcaattc aactaagtca agatcttcaa tctttagatc
     5101 tgcatatttt aatgcgttta agatagctgg tgttggacca agtcccataa ctcgtgggtc
     5161 aactccacct tgtccaaatc cgataacttc agcaattgga gttaagttat atttcttaac
     5221 tgcatcttca ctagcaacga tagtaaatga agcaccatca ttgataccag aagcattacc
     5281 tgcagttaca gttccatctg cattgaaggc tggacgtaat ttagctaatt tttctaagct
     5341 tgttttacga gggtgttcat ctgtatcaaa tactacttca cctttacgag tcttataagt
     5401 gattggaacg atttcatctt tgaatttacc tgcagcaata gccgctaaag ctttttcttg
     5461 agaagcaaat gcgaattcat cttgtgcctc acgagaaatg ttatattttt tagcgatgtt
     5521 gtcagctgta catcccatgt gaattccata cattgcatct gttaaaccat cgtgtaacat
     5581 agcatccact aatttttggt ctcccatctt agttcctgta cggtttttat agctcaacat
     5641 aaatggagca cgagtcattg attcaacacc accagcaaca tataagtcac caaagtttgc
     5701 ttgaatacga agtgtagctt ccataacggc ttttaaacct gaaccacata acatgttgat
     5761 tgattgcgca catacttcaa cagggattcc tgcatcaaaa gcaacgtgac gagcaatgtt
     5821 ttgtcctaaa cctgcagcaa ttacgttacc gataataact tcatctaagt tagctggatc
     5881 gatatttgct tgtttgattg tttcttttaa aacagttgct ccaacagtag ctaaatctac
     5941 atcagttaaa cttcctaaaa aagtaccaat tgcactacgt ttagcagcaa caatataagc
     6001 ttttctcatt tgtataccta ctttctactc tgaatcgaga ttcacttttt acatgcttag
     6061 tctagtcctt aaaatagggg atgtcaataa cttgtttaat ttttcacatg ttaataatac
     6121 ataacatata ttaataagat agggcattta acacaatttg ttaaaatatt aacaaaatca
     6181 ttgacgaggt tcaaaaaagt atgtagaata tgactgtgtg aaaagtttaa catactttct
     6241 catgctatcg atgtcctaat tcgaatagtt caagaaactg gaaaggagaa cttcatttat
     6301 ggatttcaat ttaacagaac aacaattaat gatgcaaaag ttgttcagag aagttgcgca
     6361 aaacgaagta aaagaaaacg cagcggatgt tgacgaaaac gaaagattcc ctaaggaatc
     6421 tgtagaagtg atgcgtcaag caaaaatgct tggtattcct tattcacgtg aatacggagg
     6481 agctggcgca gattatttat gctatatttt agcaatcgaa gaattgtcta aaacatgtgc
     6541 aacaagtggt gtagttttat cagctcacac ttcattaggt acttggccaa tcgctcaatt
     6601 tggtactgaa gaacaaaaac aaagattctt aacagattta tgtacaggta agaaattggc
     6661 tgcatttggt ttaactgaac caaacgctgg tacagatgcg gctggacaac aaactacagc
     6721 tgtattagat ggtgacgaat atgttatcaa tggtacaaaa atcttcatta caaatgctgg
     6781 agaagccgat gtatatgtaa tttttgccat gacagacaaa acaaaaggaa ctcgtggaat
     6841 ttctgctttc attcttgaaa aaggaactcc aggattcaca ttcggtcttc acgaaaagaa
     6901 attaggtatt cgtggatctg ctacatgtga attaattttc aataatgtac gtattccaaa
     6961 agaaaactta ttaggaaaag aaggtatggg attcaagatt gccatgcaga cacttgatgg
     7021 tggacgtatt ggtatcgctg ctcaagcatt aggaattgct caaggtgcaa tcgatgaagc
     7081 cgttgcttac gtaaaacaac gtaaacaatt tggtcgtcca attgctaaat tccaaaacac
     7141 tcaattccaa ttagctgata tgcaaactaa aacagatgca gctcgttggt tagtatattc
     7201 tgcagctact gctaaacaag aaggtcgtcc ttatactcaa ttagcagctg aagcaaaatt
     7261 atttgctgct gaaacagcta tggaaattac aacaaaagct atccaattat taggtggata
     7321 cggatatact cgtgatttac cagtagaaag aatgtttaga gatgctaaga tcactgaaat
     7381 ctacgaaggt acttcagaag tacaacgtat ggtcatctct ggcgctatgt tgaagtaaga
     7441 aaggaattgg tgttgaaaaa tgaaaattgt tgttttagaa aaacaagttc cggattcaac
     7501 tgaaattaca gttgataaaa aaactggtgc gttaattcgt gacggtgttc ctgcaatcac
     7561 aaacccagat gatttagctg gtttggaagc tgcattggaa ttaaaagaca aaaaccctga
     7621 cactgaagtt gttgtaatga caatgggatt acctaaagct gctgaaatgt taaaagaagg
     7681 tgtagctatg ggtgctgaca aaggtgtttt attaacagac cgtgtattag gtggatctga
     7741 tacatgtgct acaagtatcg ctttagcggc tgctttgaaa aaagtaggat tcgacttagt
     7801 tatcgctggt cgtcaagcta tcgatggtga tactgctcag gtaggacctc aaactgcaga
     7861 acgtttggat attcctcaag ttacttatgt agatgaaatc ttagaagtaa aagaagattc
     7921 tatcactgtt aaacgttcat tagaagatgt tgaacaaatc gtaacagcta agttaccatg
     7981 cgttataaca acattatcag gaatgaacaa acctcgttac atgcaatgtg acaacatcgt
     8041 tgacttatgt aaagaagaca agattgaagt tttaacaaat gctgaattag gaatcgatcc
     8101 tgaaacagtt ggtttcaaag gttctcctac atccgttaaa actactttca caaaagatgt
     8161 tactgacaaa acagaagtat tcacattgtc tgagcaggaa actgcagata tgattgctaa
     8221 gactttgaaa gaaaagcaaa tcattacaaa gtagggagga tgaagtagaa tggctcgatt
     8281 tgaagaatat aaagatatct tcgttttcgt tgaacaagaa aacggaaaca ttgcagaagt
     8341 aagttatgaa cttatttcag aagcaagaaa actagttgct tctatccctg aatttggata
     8401 caaagtagtt ggtgtattgc ttggagaaaa tgtaaaagaa aaagctcaag aagttattag
     8461 tcatggagct gacaaagtaa tcgttgttga tgatgctttg ttaaaagact attcaactca
     8521 attctatgct gatgctttaa cacaagttgt tttagctcac aaaccagatt caatgttaac
     8581 tggtgccact ccattaggac gtgacttagc accacgtgta gctgctcgtt taaatgtagg
     8641 tttaactgca gatgctacaa aaattgaagt tgacttagaa tatgctaaag aaactggtga
     8701 atcattatta gacgtaactc gtccaacatt cggtggtaac ttatttggta caatcgtatg
     8761 taaagacact cgtccacaaa tggctacaat cagaccaaaa gtattcgaat tggctccaac
     8821 agatacaact cgtactggtg aagttgaaga atttactcca tcatggacaa ctacagatcc
     8881 tcaagtattt gtagaaaaag taatcgaaaa agtaaaagct gatgtagata tcacaaaagc
     8941 taatatttta gtaggtgctg gtcgtggtgc tgaaaattgc ttagatgtac ttcaaaaagt
     9001 agctgacgaa ttaggtggtg tagtttgctg tacacgtgcc gttgttgaag atggattctt
     9061 agacaaagat cgtcaagtag gtcaaacagg taagactgta cgtccttcat tatatatcgc
     9121 ttgtggtatt tctggagctg tacaacactg tgctggtatg gataaatcag atttgatcat
     9181 ctctatcaac cgtgatcctc aagcagaaat cttcaatatt tctaacatgg gattcgttgg
     9241 agattgtgca actgtattac ctttattagt tgaatcaatt aaagctgcaa aagctgaata
     9301 ataaaaagga gcatatttaa aatgaaaaat gtaggtgtta ttggtgctgg aacaatgggg
     9361 caaggtattg ccaatgcatt tgctacagct ggatacaatg taactgtatg tgatattaaa
     9421 atcgaatggg cacaaaatgg aatcaacaaa attggtgcga aattagataa attagtatct
     9481 cgtgaaaaaa ttactgctga aaaagcagaa ggtatcaaag ctaacttaac tgctggtgaa
     9541 tacaaagatt tagcggattg cgatttaatc gttgaagctg ttcttgaaaa aatggaaatc
     9601 aaaaaagact tattcaagac tttagacgaa atctgtaaag aagactgcat tttcggaaca
     9661 aacacttcaa gtttaagtgt tactgaaatt gctaacggaa tcaaacataa cgttatcggt
     9721 atgcacttct tcaacccagc tgatcgtatg aaattagttg aagttatctc tggtgaaaat
     9781 actccagttg aaacaaaaga agctatcatc gaatgctcta aatcattagg aaaaactcca
     9841 gtagaagttg ctgaaggacc tggattcgta gttaaccgta tcttgattcc aatgatcaat
     9901 gaagcttgct tcatttacca agaaggattg gcaagtgttg aagatatcga tacagctatg
     9961 aaacttggag ctaaccaccc aatgggacca ttggctttgg gtgacttaat tggattggac
    10021 attgttttag atgtaatgga agtattatat actgaaacag gagattctaa atatcgtcca
    10081 tgtacattat taaagaaaat ggttcgtgct ggaaaattag gtcaaaaaac taaacaagga
    10141 ttctacagct acaactagtg aaataactta agtgaaacac tcgttgtttc actttttatt
    10201 taaatattag aggagaaaaa tcatggaaaa cgtattatta gaaaaacaag gtcacgtttc
    10261 aattatcaca atcaatcgtg aaaaagcatt aaatgcatta tcaacagctg ttttaaatga
    10321 tttagaagaa gctgttaaca ccgtttcagc tgacaaagac acatattgtg tagttatcac
    10381 aggtgctgga agcaaatctt tcgttgctgg tgcagatatt gcagaaatga aagacaaaac
    10441 ggttgaagaa gcggctgaat atggagctta tggaaataag atcttccgtc aaattgaaac
    10501 tttacactgc ccagtaattg cagcagtaaa tggttttgca ttaggtggag gatgcgaatt
    10561 aagtatggct tgcgacattc gtatcgctag tgaaaatgct atttttggac aaccagaagt
    10621 aggtttagga atcactccag gttttggtgg aactcaacgt ttagcacgtt tagtacctgc
    10681 tggaatcgct aaagaaatga tctttacagc tcgtaacatc aaagccgaca aagcattggc
    10741 tattggatta gtaaatgctg ttgttcctca agaagaatta atggctactg cattaaaaat
    10801 ggctaatgga atctgcaaaa atgctccaat cgctgttgca caatctaaaa aagctatcaa
    10861 tgctggactt caaacagata tggattcagc tatcgctatc gaagtaaaag atttctctga
    10921 ttgctttgct actgaagacc aaacatatgg tatggaatgc ttcgtaaaca aagtaaaaga
    10981 aaaagaattt aaaaacaaat aatttatttg aaaggatgat ttaaatgtca aaagtaatgt
    11041 cagcccaaga agccatcagt ttaatcaaag atggcgacaa aattggtgtt ggtggattta
    11101 ttggaatggg tcaccctcaa gaacttagtg ttgccattga aaatagtttc ttagaaactg
    11161 gacatcctag agatttaacc gttatgttct ctgccggtgt aggagatggt acaccagatt
    11221 taggattgaa ccatatgggt catgaaggat tgttaaaaag aatcattggt ggacactggg
    11281 gattgattcc tactttccaa aaattagttt ttgaaaacaa agttgaaggc tataacttac
    11341 ctttaggaac aatcagtttg atgttccgtg aaattgcagg acaccgtcca ggtgttatca
    11401 caaaaattgg tttgaaaaca tttatcgacc ctcgtctaga aggtgcgaaa atgaatgaac
    11461 gcactaaaga agatttagtt gaattgatca atatggatgg acaagaatgg ttacgttata
    11521 aatcattccc attagatatt gcgttgattc gtggtacata tgcggatgaa gatggaaact
    11581 gctcaatgtg taaagaagca gcaactctag attcaatcag tattgcgcaa gctgttaaaa
    11641 acagtggtgg taaagttatt ctacaggttg aaaaaattgt agaacgagga tcattaaaac
    11701 caatggatgt taagattcca ggaatttatg tggatgcgat cgttgttgca caacctgaaa
    11761 accattggca aacgtattca gatccataca acccatcatt ctgtggagaa attcgtgtac
    11821 cagttgattc aattgaacca atgccattaa acgcacgtaa agttgtttgt cgtcgtgctg
    11881 ctatggaatt ggatccatca gctgttatca acttaggaat tggtatgcca gaaggtatcg
    11941 caaatgttgc caatgaagaa ggactaccag gacttaaaat gacagttgaa gctggaggaa
    12001 tcggtggtgt tccaatgaat gggactgcct ttggtgcttg tactaaccca gatgcaatca
    12061 ttgatcaaac atatcagttt gacttctatg atggtggtgg attagatcaa gccttcttag
    12121 gacttgcaga atgtgatcaa aaaggtgata tcaacgtttc tcgttttgga ccaaaaattg
    12181 caggatgtgg tggatttatt aatatcacac aaacttcacc ggtcgttgtc tattgtggaa
    12241 catttacagc taaaggttta aaagaaacag ttggaa
//
LOCUS       DBMDWL010000051        12176 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1772_length_12176_cov_4.136894, whole genome shotgun sequence.
ACCESSION   DBMDWL010000051 DBMDWL010000000
VERSION     DBMDWL010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12176)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1772_length_12176_cov_4.136894"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="ACZPDN_04980"
     CDS             complement(<1..1080)
                     /locus_tag="ACZPDN_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298007.1"
                     /translation="MLKNLKRAAAIVLSFAMAIQFGLANSYYVNAEEEPVEPQQEQTT
                     TVENNSEVQEEDANEVAVPETKSVELSYVAEDGTILQAATYRDFDLNYGLNTDSSVML
                     NFDGYTLKDVIVNNSQTVSADQASLSVTSDLASVQFVYKKIVIETENQTTEPAKTEEK
                     NEDDAEADPDPETEVKEELPEYPAFDQSEMAGNVVVRATAAEGVLPRDSKLVVKRITR
                     KAILNAVEETVSEKNMEVESAVALDVTIQSKDGVEIQPNGFVNISFENVATTAQETNV
                     YHVTNDASTVTEVATNAQSFDADHFSIYVITNENKVPLTTFNFVANGTTVSTQIVKSG
                     DTLIAPTAPDMIGQAFVGWVDEAGNS"
     gene            complement(1231..1713)
                     /locus_tag="ACZPDN_04985"
     CDS             complement(1231..1713)
                     /locus_tag="ACZPDN_04985"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR1298008.1"
                     /translation="MNKLYWAKVKPDAKVPQKRYEDAGYDLYPCFEEDYMVIEPHETK
                     LVPLGVASAFDPEFVMILKERGSTGTKGIAQRSGVIDSGYRGEYMAPVTNLNTKRVVI
                     KKQDVEYKEDAIIYPYEKAICQGVLLRMPQLESEEISFEKLQSMESERMAGKLGSSGK
                     "
     gene            complement(1706..2167)
                     /locus_tag="ACZPDN_04990"
     CDS             complement(1706..2167)
                     /locus_tag="ACZPDN_04990"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HIR1298009.1"
                     /translation="MCKRIVGLDPIVFKDSRVLILGSMPSVISLKEQMYYANKTNRFW
                     KVLEAIYQENDKIKLLKTSHIALWDICHSCIRKTSADSEIKDIEPNDIQELLNRYKNI
                     EKVICNGRTSQNTMQKYFPNIQTVYCPSTSSANAQFSLEQLIEIYKKEIYE"
     gene            complement(2152..3450)
                     /gene="rimO"
                     /locus_tag="ACZPDN_04995"
     CDS             complement(2152..3450)
                     /gene="rimO"
                     /locus_tag="ACZPDN_04995"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1298010.1"
                     /translation="MKIGFVSLGCCKNLVDSEQIMGMIKRGGHEIVANPKDAQAIIVN
                     TCGFIESAKEESINTIFKMAQYKERNLEKLIVCGCLAQRYTDTLREEIPEIDAVIPIR
                     EYDTLASQLQKILGSDTLLNKAERVISGNPWQAYVKISDGCSNRCAYCAIPLIRGDQK
                     SRTIEDIMEEVNHLASIGVKELTLIAQDTTKYGLDNYGELMLPELLRQVSKVEELHWI
                     RVLYMYPDEIVDDVLQAMSESEKIVPYFDIPMQHANNRLLKLMNRRGPKEDVLKVVDK
                     IHTMFPNATLRTTMIVGFPTETEEEFEELVDFIKEIKWDRMGAFTYSKEEDTPAYDMD
                     GQIDEEVQNDRLARLMAVQEEISKEKNQEKIGKVIEVLVEEKEGLVDRYRGRSAADAP
                     DEVDGQVIFTSEEPLELGTFVKVKITDAKSYDVYGTCVNE"
     gene            complement(3447..4385)
                     /locus_tag="ACZPDN_05000"
     CDS             complement(3447..4385)
                     /locus_tag="ACZPDN_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR1298011.1"
                     /translation="MRINEITQEQKVNVKCLISKCDKGKTVKDTPYLSLILEDATGVL
                     DAKFWNLTNEQIEQYKAGQVVEVIGDSIIHRNAVQLRVRKMTVLEDEDISNYVRLAPM
                     TRTEMEEEVKILMNEITNTNLYCVVEEVLEETKDLFYTYPAATRNHHNFVGGLAYHSI
                     SMARVALDICRQYTFLDKGLLIAGILMHDVGKIDELSGPVLPEYTNEGNLLGHISIMN
                     NRLDRVAEKLGVNDKECVLLLKHMVLAHHGKMEFGSPVLPMIPEAEVLTLLDNLDARM
                     YMMKQSLDTTQPGHFGPRVFALEGRMIYHRKGEDEE"
     gene            complement(4382..6349)
                     /locus_tag="ACZPDN_05005"
     CDS             complement(4382..6349)
                     /locus_tag="ACZPDN_05005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1298012.1"
                     /translation="MRIGLFTDAYLPDINGVVSSVATLKHALEELGHTVFIITNHKGV
                     KIAEDEDGHILRLPGLEIKKFYGYKMSSPVQFTADAYIEKMDLDVIHIQTEAGVGMFG
                     RQMAKVLHIPIVYTYHTMYEDYTHYFNPLEFDSVEKLGKSFIRAFSRVMANGSQAVIA
                     PSEKTKQALIQYGVMAPIYIVPTGLDLSEYDRKNLDETRVKRIREELGFSQEDHIVVF
                     VGRIAKEKAIEIPIEAICKNKDPHLHLVIVGGGTDMEYYQDLAKKYNVENHVHFTGKI
                     PKEDIAYYYAAFDCFVSASLSETQGMTYIEALASGLLVFGRRDEVLKDLVDEGKSGYY
                     FDDANELSQKWDVFFSKSKAERDALREYCVSKTAPYTESMFAHKALSVYNQAIDDYKR
                     AYHVERIRMVDDFVRLTVIRDCDNEPVKVMIPTEDFFDLKITKDTMLDAYLVDNYLDM
                     QLFYKAFELMKKRVLSNDYTSYQMVQYGKQYLSLDEDQAKEIVNEFMERHWIDDKDYA
                     FDKAQAWHSYGQPKMQIYSKLKKAGVQDDMIDAALACLDEETERSNATKLARRLTHSI
                     KEQSSRMQRQTLVNKLVTKGYSFEIAKQVSESIELDENDDEALQRTIAKAKRLYATFD
                     QPKRNQKIQTYCVRKGFNISAIKEVLEGENE"
     gene            complement(6353..7393)
                     /locus_tag="ACZPDN_05010"
     CDS             complement(6353..7393)
                     /locus_tag="ACZPDN_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR1298013.1"
                     /translation="MKKQKYALNLLLILALTGFALWFALKDNYQQVVKCISQMNLLSL
                     VIVLLWGVLFTAVWGFVYYVLGRKYTENYTPGKGMLVAFIGSFFSGITPSSTGGQFVQ
                     AYIMKKQGIKVSDGASLLWADFIIYQTTMMVYVSILFLLRFAYYSAQSAWFNIILLGY
                     IVNAVVVVALYTIALFPNLYIKLSRILVQLLTKLHILKNPEKTLDSWTLQVTSFTREI
                     KVLAKDKKSIFLCVCINIVRLSLYYSLPFVIALALHIPLKMNEFIDVMALSSFVTMAN
                     SFIPIPGASGGTEVVFSLLFNSLMKDLTGAVLVLWRFSTYHIVLIIGGILFVFVKNYY
                     ERKESKINLEGM"
     gene            complement(7402..8499)
                     /locus_tag="ACZPDN_05015"
     CDS             complement(7402..8499)
                     /locus_tag="ACZPDN_05015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1298014.1"
                     /translation="MRYNNEDERSVIFMEDKIRIIMYSSADKVDGQGVGSAYEEQGNL
                     IKEGASDIFEVGINNWIKTPDIQHFHTVDPTFYVKMQNHKAANIAYCHFLPDTLEGSL
                     KIPAPLLNVFRSYLIQFYKSADRLVVVNPSFIDALVEYGIDREKIYYIPNYVSKEKFY
                     RKSDDECAAIRKKYGVGKDAFVVLGAGQVQTRKGVLDFVNVAKEMPDVQFVWAGGFSF
                     GRITDGYEELKKLQENPPENCKFLGIVPREEMVDLYNMADVLFVPSYNELFPMTILEA
                     VNLHVPLVLRDLDLYKDILFDRYMHANDNDGFKNCLLKLKNDSDVYAKYQNESRLLSE
                     FYSKEHVLNMWREFYLSAYKDKQEELLNKKK"
     gene            complement(8743..9060)
                     /locus_tag="ACZPDN_05020"
     CDS             complement(8743..9060)
                     /locus_tag="ACZPDN_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298015.1"
                     /translation="MSLSRKERDQLAEVIQRENEMVLKVGRMVRNAFILTLAFGAVTY
                     WGWSGMTDPMFPNIPMSVRNVAKWIALIGLILSGLFTVLGFISHRNGKKSVLKKIDLY
                     EEK"
     gene            complement(9153..9983)
                     /locus_tag="ACZPDN_05025"
     CDS             complement(9153..9983)
                     /locus_tag="ACZPDN_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1298016.1"
                     /translation="MKKISKKTLNSSFWRWWYGNLTCFSHEHMQTFGYMWSMLPIIKE
                     LYPTREEQSEKMTTYYPFFNTEPQIGSIVVGITAGLEEARANGAEGIDDEMINGIRAG
                     LMGPLAGIGDSLIVGTYIPILLGIALGFAEGGSPLGPLFYIIVWNATSIWFQKFVYFK
                     GYELGGSAVELLVGQKATAFRESVLVMGQVIVGAMAASWVSISTNLVIAQSYDAETGK
                     LTDVTLGSKLDGAFPKILTMLFVLFAWWLMAKKNITPIKVLLLFVVIGFVGSVVGFLG
                     "
     gene            complement(9986..10825)
                     /locus_tag="ACZPDN_05030"
     CDS             complement(9986..10825)
                     /locus_tag="ACZPDN_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1298017.1"
                     /translation="MSWLQAILIGLMSCTAASTIACLGTTVGNYTLNRPLVASLFVGL
                     ILGDVSGCIQIGIPMQVMWIALVTPGGTVASDLRAVSYIGIPLAYVGAKAAGMDFGGS
                     DAQGLASSISAMTGVIGITLFYGTAMMNLIWQHWGWAQLDKGNLDCLGKVDVVLPLIS
                     HFVLSFLPVTLLCYYGSGAVAELFQSLNTEVWYVKAILSVGSVLPAVGIGILLKSVVS
                     KTSDLIYFMFGFALAASMGLTLIAATVVGGVFALINYQMTMMKDTGAVGGASADYDDE
                     EDI"
     gene            complement(10880..11371)
                     /locus_tag="ACZPDN_05035"
     CDS             complement(10880..11371)
                     /locus_tag="ACZPDN_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIR1298018.1"
                     /translation="MISFIRVDDRIIHGQIVTRWSKEYPCDGIIAVNDKAATTPVLTQ
                     SFKASTDKKVFVWTKEHWREKAETVKNSNKKYFLITKNPVDMKELLVDDGFVPNDVKT
                     VVIGPCNDRPGATKLGQNQSITQEEAEACEALTNAGYTVLFALLKETAIGTWDKFRSK
                     FGY"
     gene            complement(11427..11855)
                     /locus_tag="ACZPDN_05040"
     CDS             complement(11427..11855)
                     /locus_tag="ACZPDN_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1298019.1"
                     /translation="MKYVVMVSHGEFAPGLHSAVKMMTGDREDVLSTSLKDGMSADEF
                     ASNFAELVKDFKADDQVILLADILSGSPFTNALNVLDQKGLLQNTLVIAGMNMPLAIT
                     AVLMKDNFDNVQDLKDTLLSEGRTGLTDFTVASDDDDEDL"
BASE COUNT         3849 a         2711 c         1616 g         4000 t
ORIGIN      
        1 tgaattacca gcttcatcaa cccaacctac aaatgcttgt ccaatcatat ctggagctgt
       61 aggagcaatt aatgtatccc cagacttaac aatctgtgta cttactgttg taccattcgc
      121 aacaaagttg aatgttgtta gtgggacttt attttcattc gtaataacat aaattgagaa
      181 gtgatctgca tcaaagcttt gtgcatttgt agctacctca gttactgtac ttgcatcatt
      241 cgtaacatgg taaacatttg tttcttgagc tgtagttgct acattttcaa aactgatatt
      301 aacaaatcca tttggttgaa tttcaacacc atctttgctt tgaatcgtaa catctaatgc
      361 tacagctgat tcaacttcca tattcttttc agaaaccgtt tcttcaactg catttaagat
      421 agcttttctt gtaatacgtt ttacaacaag tttagaatct cttggtaaaa caccctcagc
      481 tgctgtcgca cgaacaacaa cattaccagc catctcactt tgatcaaatg ccggatactc
      541 cggcaactcc tcttttactt ccgtttccgg atccgggtct gcctcagcat cgtcttcatt
      601 cttttcttcc gtcttagcag gttcagttgt ttgattctct gtttcaataa ctattttctt
      661 ataaacaaat tgaacactcg caagatctga agtcacactt agacttgcct gatcagcact
      721 cactgtttgt gaattattga cgattacatc ttttaatgta taaccatcaa aattcagcat
      781 aacactacta tctgtattta agccgtaatt taaatcaaaa tcacgatatg tagctgcctg
      841 taaaattgtt ccatcttcag ccacatagct taactctaca gatttcgttt ctggcactgc
      901 aacctcattt gcatcctctt cttgtacttc actattattt tctacagtcg tagtttgttc
      961 ctgttgtggt tcaacaggct cttcttctgc atttacatag taagagtttg ctagtccaaa
     1021 ctggattgcc atcgcaaatg ataatacaat tgctgccgct ctcttcaagt ttttcaacat
     1081 acaaagccct cctttttgat atggttgtaa tccctttcat tatctgattt tagtataggc
     1141 tttgtatggt tatagaccgg ttatataatt taaaaatcat cttttttcta aaaaaagatc
     1201 tattgccctt atgacaatag atccatttca ctattttcct gaacttccaa gttttccagc
     1261 cattctttca gattccatag actgaagttt ttcaaaggaa atttcttcac tctctaattg
     1321 aggcatacgc aataatacac cctgacaaat tgctttttca taaggataaa taatcgcatc
     1381 ttccttatac tctacatctt gctttttaat aactacacgc tttgtattca aatttgtaac
     1441 tggagccata tattctccac gataacccga atcaatcacg ccacttcttt gtgcaatccc
     1501 ctttgttcct gttgaaccac gctcttttag aatcatcaca aactctggat caaatgcact
     1561 cgcaacacct aatggaacta actttgtttc atgaggctca attaccatgt aatcctcttc
     1621 aaaacatggg tataaatcat accctgcatc ttcataacgt ttttgcggaa ccttagcatc
     1681 cggtttaacc tttgcccaat ataatttatt cataaatctc cttcttatat atttcaatca
     1741 attgttctaa tgaaaactgt gcattggcac tggaagtcga aggacaatat accgtttgaa
     1801 tattcggaaa atatttctgc attgtattct gacttgtcct tccattacaa atgacctttt
     1861 caatattctt atatctattc aacaattcct gtatatcatt tggctctata tctttgattt
     1921 cactatccgc actcgtcttt cttatacacg aatgacaaat atcccaaagt gcaatatgtg
     1981 atgtttttaa tagttttatc ttatcgttct cttgataaat tgcttctaag actttccaaa
     2041 atcggtttgt cttattcgca taatacatct gttcttttaa actaatgaca cttggcattg
     2101 aacccaaaat caatactcta gaatctttaa atacaatggg atccaatcca actattcgtt
     2161 tacacatgtt ccgtaaacat cataagattt ggcatctgta atctttacct tcacaaaagt
     2221 acccaactct aaaggttctt cacttgtaaa gatgacctga ccatccactt catccggtgc
     2281 atccgcagca cttcttccac gataacgatc caccaaacct tctttttcct caactagaac
     2341 ttctatgacc ttaccaatct tttcttgatt cttttctttt gaaatttctt cctgcacagc
     2401 catcaatcta gccaaacgat cattttggac ttcttcatca atctgaccat ccatatcata
     2461 agcaggtgta tcttcttcct tcgaatatgt gaaagcaccc atacgatccc attttatttc
     2521 cttgatgaaa tctacaagtt cctcaaattc ttcttctgtt tctgtaggga aaccaacaat
     2581 catagttgta cgcaaagtcg catttggaaa catcgtatga atcttatcca caacctttaa
     2641 tacatcctct tttggacctc tacgattcat taatttcaat aaacgattgt tcgcatgttg
     2701 cattggaata tcgaaataag gaacgatttt ttcactttcg ctcatagcct gcaatacatc
     2761 atccacaatt tcatcgggat acatatatag aacacgaatc cagtgaagtt cttctacttt
     2821 agatacttgg cgcaacaatt caggaagcat caattcacca taattatcca aaccatattt
     2881 tgtcgtatcc tgcgcaatca atgttaattc cttaacacca atacttgcca aatgattgac
     2941 ttcttccata atatcctcaa ttgtacgtga cttttgatct ccacgaatca aaggtattgc
     3001 acaataggca caacgattgg aacaaccatc actaattttt acataagcct gccaaggatt
     3061 ccccgaaatc actcgttcag ccttattcaa taatgtatcg gaacccaaaa ttttttgtag
     3121 ttgacttgcc aaagtatcat attcacgaat cgggataacc gcatcaattt caggaatttc
     3181 ttcccttaat gtatctgtat atctttgtgc taaacaacca cacacaatca acttctctaa
     3241 gttacgctct ttatattgcg ccatcttaaa aattgtatta atactttctt ctttggcact
     3301 ttcaataaaa ccacaagtat tcacaataat agcctgtgca tcttttggat tagccacaat
     3361 ctcatggcca ccccttttga tcatacccat gatttgttct gaatccacca agtttttaca
     3421 acatcccaat gatacaaaac ctattttcat tcttcatctt ctccttttcg atgataaatc
     3481 atacgacctt ctaacgcaaa aacacgaggc ccaaaatgac ctggctgcgt tgtatctaaa
     3541 ctttgcttca tcatatacat acgagcatct agattatcta acaaagtcaa tacttctgct
     3601 tctggaatca taggaagaac aggagatcca aactccatct ttccatgatg tgccaaaacc
     3661 atatgcttca acaataaaac acactcttta tcgtttacac ccagcttttc agccacacga
     3721 tccaaacgat tattcataat actaatgtgc cccaacaaat tcccctcatt tgtatattca
     3781 ggaagaaccg ggccactcaa ctcatcaatc tttccaacat catgcatcaa gatacccgca
     3841 atcaataatc ccttatccaa gaatgtatat tgtctacaaa tgtctaaagc aacacgcgcc
     3901 atactaatag aatgataggc aagtcctccc acaaaattat ggtggtttct tgtagctgct
     3961 ggatatgtat aaaataaatc ttttgtttct tctaacactt cttcaaccac acaatacaaa
     4021 tttgtattcg tgatttcatt catcaaaatc tttacttcct cttccatttc cgtacgagtc
     4081 ataggagcaa ggcgtacata attagaaata tcctcatctt ctaagactgt catctttctt
     4141 acacgcaact gaactgcatt tctatgaatg atagaatctc caatcacttc tacaacttgt
     4201 ccagctttat attgttcaat ctgctcattc gttaaattcc aaaattttgc atccaataca
     4261 cccgttgcat cctctaaaat caaagacaaa tacggcgtat ccttcacggt ttttcccttg
     4321 tcacattttg aaatcaaaca cttaacattt actttttgtt cctgtgtaat ctcattaatt
     4381 ctcattcatt ttccccttcc aaaacttctt ttatagccga tatattaaac cctttacgaa
     4441 cacaatacgt ttgaatcttc tgattacgct ttggctgatc aaacgttgca tataaacgct
     4501 tcgctttcgc aattgttctt tgtagtgcct catcatcatt ttcatccaat tcaatcgatt
     4561 ctgagacttg tttggcaatc tcaaacgaat atcctttcgt aaccaattta ttgactaaag
     4621 tctgtctttg catacgactc gactgttcct tgatcgaatg cgttaaacga cgagctaact
     4681 tggttgcatt gctacgctct gtttcttcat caagacaagc tagtgctgca tcaatcatat
     4741 catcctgaac ccctgctttt tttaattttg aataaatctg cattttgggc tgtccatacg
     4801 aatgccaagc ttgtgcctta tcaaacgcat agtctttatc atcaatccag tgccgttcca
     4861 taaattcatt cacaatctct ttagcttgat cttcatccaa acttaaatat tgcttaccat
     4921 actgtaccat ctgatacgat gtataatcat ttgaaagcac acgcttcttc atcagttcaa
     4981 acgctttata gaataactgc atatccaaat aattgtcaac caaataggca tctaacattg
     5041 tatccttagt gatttttaaa tcaaagaaat cctctgtagg aatcattact ttgactggtt
     5101 cattatcgca atctcgaatc acagttaaac gcacaaaatc atccaccata cgtatgcgtt
     5161 ccacatgata tgcacgctta taatcatcaa ttgcctgatt ataaacactc aaagccttat
     5221 gtgcaaacat actctctgta taaggagctg tttttgatac acagtattct cttaatgcat
     5281 ctctttccgc tttggattta ctaaagaaaa catcccactt ttgacttagt tcattggcat
     5341 catcaaaata atatccagat ttgccttcat ccaccaaatc tttcaacact tcatcacgac
     5401 ggccaaaaac taaaaggcca ctagccaaag cctcaatata tgtcatcccc tgtgtttctg
     5461 ataaactagc tgacacaaaa caatcaaaag ctgcataata ataggcaata tcttccttag
     5521 gtattttgcc agtaaaatgc acatgattct ctacattata tttcttagcg agatcctgat
     5581 aatactccat atccgtacct ccacctacaa taaccaaatg taagtgcgga tccttatttt
     5641 tacaaatcgc ttcgatggga atttcgatcg ccttttcctt agcaatacgt cctacaaaga
     5701 caacaatatg atcttcttga ctaaaaccta gctcctcacg aatcctttta actcttgttt
     5761 catccaaatt tttacgatca tattccgata aatctaaacc cgttggaaca atatagatag
     5821 gtgccatcac accatattga atcaatgctt gtttcgtttt ttcactaggt gcaataacag
     5881 cttgagagcc attggccatg acacgtgaaa atgcacgaat aaaacttttg cctaattttt
     5941 ctacagaatc aaattcaaga ggattaaaat aatgcgtata atcctcatac attgtatgat
     6001 acgtatacac aattggaata tgtaatacct tagccatttg acgaccaaac atacccacac
     6061 ccgcctcagt ttgaatatga atcacatcta gatccatctt ttctatatag gcatctgccg
     6121 taaattgtac tgggcttgac attttatatc cataaaattt ttttatttca agacccggca
     6181 aacgaagaat atgtccatct tcatcttctg caatttttac tcccttatga tttgtgataa
     6241 taaacacagt gtgtcctaac tcttctaaag cgtgtttaag ggttgccaca cttgaaacaa
     6301 ccccattaat atctggtaaa tatgcatctg taaacaatcc aattcgcata cgctacatcc
     6361 cttctaaatt gattttactt tcttttcttt cataataatt cttaacaaac acaaataata
     6421 ttccaccaat aatcagcact atgtgatacg tagaaaaacg ccataataca agtacagccc
     6481 ccgttagatc tttcattaag ctattaaata ataatgaaaa gacaacttcc gtaccaccac
     6541 ttgctccagg aattggaata aagctattgg ccattgtcac aaaacttgat aaggccataa
     6601 catcaataaa ttcattcatt ttaagtggaa tatgcaaggc tagcgcaatc acaaaaggta
     6661 aagaataata gagtgataaa cgtacaatat ttatacatac acaaagaaaa atactttttt
     6721 tatcttttgc gagcacctta atttcacgag taaaactagt cacttgtaaa gtccaagaat
     6781 ccaacgtttt ttctggattc tttaatatat gtagctttgt taatagctga actaaaatac
     6841 gcgaaagctt gatatataag tttggaaata acgcaatcgt atataacgcc acaaccacaa
     6901 ctgcattgac aatatatccc aacaagataa tattgaacca cgcagattga gcactataat
     6961 atgcaaaacg caaaagaaac agaatcgata cataaaccat cattgtggtc tgataaataa
     7021 taaaatcagc ccacaacaag cttgccccat cactcacttt gattccttgc tttttcataa
     7081 tataagcctg aacaaactgt ccacccgtac tacttggtgt aatacctgaa aagaaagaac
     7141 caataaaagc tacaagcatt cctttaccag gtgtataatt ctctgtatat tttcttccta
     7201 atacataata tacaaatccc cataccgcag taaaaagaac tccccataat aatacaatga
     7261 caaggcttaa cagattcatt tgtgaaatgc actttacaac ttgttgataa ttatccttta
     7321 gagcaaacca tagggcaaat cccgttaaag caagaataag caacagattt agtgcgtact
     7381 tctgtttttt cataaaaacc cctatttttt cttatttaat aattcctctt gtttatcctt
     7441 atatgcactc aaataaaatt ctctccacat attcaatacg tgttctttcg aatagaattc
     7501 actcaataaa cgactttcat tttgatactt cgcgtataca tctgaatcat ttttcaactt
     7561 taacagacaa tttttaaaac catcattatc attcgcatgc atataacgat caaataaaat
     7621 atctttatat aaatccaaat cacgaagcac caatggaaca tgtaaattca ccgcttctaa
     7681 aatagtcatt gggaacaatt cattatagct tggcacaaaa agcacatccg ccatattata
     7741 tagatccacc atttcttctc ttggtacaat gcctaagaat ttacaatttt ccggtggatt
     7801 ttcctgtaac ttctttaatt cttcataccc atctgtaatg cgaccaaacg aaaagcctcc
     7861 agcccataca aattgtacat ctggcatttc tttggcaaca ttcacaaaat caagcacacc
     7921 cttacgcgtt tgtacctgac ctgcacccaa taccacaaaa gcatctttac ctacaccata
     7981 cttttttcga atggcagcac actcatcatc ggatttgcga tagaattttt ccttagatac
     8041 atagttagga atataataaa ttttttctcg atcaatacca tattctacta atgcatcaat
     8101 aaagcttgga tttacaacaa ccaagcgatc tgcactctta taaaattgaa tcaaataaga
     8161 acggaataca ttcaataatg gtgctggaat ctttaagcta ccttctaaag tatctggtaa
     8221 aaaatgacaa taggctatat tagcggcctt atgattttgc atctttacat agaaagtagg
     8281 atccactgta tggaaatgct gaatatccgg tgtttttatc caattgttga tacccacttc
     8341 aaaaatatca cttgctcctt ctttaatgag attgccctgt tcttcatagg cactgcctac
     8401 accttggcca tctactttat ctgcacttga atacattata attcgtattt tatcttccat
     8461 aaaaattaca cttctttcat cctcattatt atatctcatc tactatttgt atgtccaact
     8521 ttcaaaaagg caaatgaaaa gcccctttag cggggcttct caagaaagtt ttaagaaagg
     8581 gaatttgatt atgaagtgag ccactatctt tggctcaata gtatattact aaacaataaa
     8641 gtgatagatg tagttatatt atgtgaaatt atgttaacat atgacacaat tattgttgtt
     8701 tttagttaat accataataa atttaatcaa aatatacaaa ttttattttt cttcgtacaa
     8761 atcaatcttc tttaatacac ttttcttccc atttctatga gaaataaatc ctaaaacagt
     8821 aaataaaccc gataaaatca aaccaatcaa agcaatccat ttagctacat tacgtacgct
     8881 cataggaata ttaggaaaca ttggatctgt catccctgac catccccaat atgtaacagc
     8941 accaaaagca agtgttagaa taaaagcatt tctgaccatt cttcccactt ttaataccat
     9001 ttcattttct ctttggatga cttctgcaag ctgatctctt tctttgcggc tcaaactcat
     9061 aataaacgct ccttttcaaa tatacatata cttattataa gaaaaaaaga caggcctaac
     9121 aagttaagcc cgcctttaat tgtcttaagt gattatccta agaatcctac aacagatcct
     9181 acgaatccga taactacgaa caataacaat actttaattg gagtaatgtt tttcttagcc
     9241 atcaaccacc aagcaaacaa tacgaataac attgttaaga tcttagggaa agctccatct
     9301 aatttagatc ctaatgttac gtctgttaat ttaccagttt ctgcatcata agattgtgca
     9361 attactaagt tagttgaaat tgatacccaa gaagctgcca tcgcaccaac gataacttgt
     9421 cccataacca atacagattc acggaacgca gtcgcttttt gtccgactaa caattcgaca
     9481 gcactaccac ctaattcata acctttgaag tatacgaatt tttggaacca aattgaagtt
     9541 gcgttccata cgatgatata gaataaaggt cctaaaggtg atccaccttc tgcgaaacct
     9601 aatgcgatac ctaataagat aggaatgtaa gtaccaacga tcaatgagtc accaatacct
     9661 gccaaaggtc ccattagacc tgcacggata ccgttgatca tttcatcatc gattccttca
     9721 gctccgtttg cacgagcttc ttccaaacca gctgtgatac caactacgat agatccaatc
     9781 tgaggttcag tattgaagaa tgggtagtaa gtagtcattt tttctgattg ttcttcgcga
     9841 gtaggatata attccttaat aattggaagc attgaccaca tgtaaccaaa tgtctgcatg
     9901 tgttcgtgtg agaaacatgt taagtttcca taccaccaac gccagaaaga actatttaat
     9961 gttttcttag aaatcttttt cataattaaa tgtcctcctc gtcatcatag tctgcagatg
    10021 caccaccaac agcacctgta tctttcatca ttgtcatttg gtagttaatc aatgcgaata
    10081 caccaccaac aactgtagca gcaattaatg ttaatcccat agaagcagct aaagcgaatc
    10141 cgaacatgaa gtaaatcaag tcagaagtct tagatactac tgattttaac aagataccaa
    10201 tacctactgc aggaagtact gatcctactg ataagatggc ttttacatac catacttcag
    10261 tatttaagct ctggaacaat tcagctacgg ctccagatcc atagtagcaa agtaaagtaa
    10321 ctggcaagaa tgataataca aagtgtgaaa ttagtgggag aacaacatca acttttccta
    10381 aacaatctaa gtttccttta tccaattgag cccagcccca gtgttgccaa ataaggttca
    10441 tcatagctgt tccgtagaat aaagtaatac cgattacacc agtcatggct gagatactag
    10501 atgctaaacc ttgagcatca cttcctccaa aatccatacc agcggctttt gctccaacat
    10561 aagccaatgg aataccaatg taacttacgg cacgtaagtc agatgcgact gttccaccag
    10621 gtgtaaccaa tgcgatccac attacctgca ttggaatacc aatttgaata catccagata
    10681 catcacctag gatcaaacct acgaataatg atgcaaccaa aggacggttc aaagtgtagt
    10741 tacctactgt tgtacctaag caagcgatag tactagctgc tgtacaactc ataagtccga
    10801 ttaaaattgc ttgtaaccaa ctcattataa atctctcctt tttcttttat ttatctcaat
    10861 ctgtttacta ttcccactat tagtaaccaa atttagatct aaatttgtcc caagtaccga
    10921 ttgcagtttc ttttaataat gcaaacaata ctgtgtaacc agcgtttgtc aaagcttcac
    10981 atgcttcagc ttcttcttga gtaattgact ggttttgacc taattttgta gctcctggac
    11041 gatcattaca tggtccgata actactgttt taacatcgtt aggtacgaat ccatcgtcta
    11101 ctaacaattc tttcatatcc actgggtttt tagtaattaa gaaatatttt ttattagaat
    11161 tcttaactgt ttctgctttt tctctccagt gttcttttgt ccaaacaaat actttcttgt
    11221 ctgtactagc cttgaatgac tgtgtcaata caggtgtagt tgcagctttg tcgttaactg
    11281 cgataattcc atcacaaggg tattctttag accatcttgt aacaatctga ccgtggataa
    11341 tacgatcatc aacacgaata aaactaatca ttatatgtcc tcctttattc tgtttatgcc
    11401 ttcgctatat gcgttatagc caaatcctat agatcttcgt cgtcatcatc agaagcaact
    11461 gtaaaatcag ttaatcctgt acgaccttcg cttaataatg tatcttttaa atcttgtaca
    11521 ttatcaaagt tatctttcat taatacagca gtgattgcaa gaggcatatt cataccagca
    11581 attactaaag tattttgaag caatcctttt tgatccaata cattcaatgc atttgtgaat
    11641 ggagatccac tcaaaatatc agctaataaa atgacctggt catctgcttt gaaatcttta
    11701 accaattcag caaagttaga tgcaaattcg tctgcgctca ttccgtcctt taagcttgta
    11761 gacaatacat cttcacgatc tcccgtcatc attttaacgg cactgtgtaa tccaggggcg
    11821 aattcgccat ggcttaccat aactacatat ttcattattt tttcctccgc gttttcttat
    11881 actcacattt tataataaat atgtcagcgt tttcaagtgt tattacacat aatttcacac
    11941 atttatacga cttttgaaca tgagcatcac atagaataca cattcattat aacctgtatg
    12001 aaaacctttt caatttccat atgtcaccac ttttagatga caaatgtcat ttttgagaag
    12061 caaacaaaaa acagcagaaa tttaactttc tactgttcct taactttgca gaatttgtat
    12121 aactaaaaaa taaaagaagc attagtagct gaattcacca atgcttctgt caatat
//
LOCUS       DBMDWL010000052        12155 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1780_length_12155_cov_3.262983, whole genome shotgun sequence.
ACCESSION   DBMDWL010000052 DBMDWL010000000
VERSION     DBMDWL010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12155)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12155
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1780_length_12155_cov_3.262983"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="ACZPDN_05045"
     CDS             <1..496
                     /locus_tag="ACZPDN_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298020.1"
                     /translation="LENYNKQWIEYAENCNVSFKAYTKKGDLQILGIADFDGFYKRFT
                     TLGAKKYAYDDENDQLHITIAGVPKKLGAKLLGKIENFKVGMHFMVGADGTLEDRQAW
                     KKRLLYNDTDNFDLTIDGNILHIGTYIAMERTSYELSITDEYEELISSLKNDEIYEKD
                     DIWG"
     gene            667..1041
                     /locus_tag="ACZPDN_05050"
     CDS             667..1041
                     /locus_tag="ACZPDN_05050"
                     /inference="COORDINATES: protein motif:HMM:NF037548.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298021.1"
                     /translation="MSAKVIKTSEGMSARKVLSYTSRNDAIPMKELAKGTIIPFKGYI
                     EQEIINEITGEVFNSLLIISEPDESGCDALYATRSESVMRSLSDIIDTLTDMGDTDPF
                     SVKVDKLKSKNGREFITLSLAD"
     gene            1055..1318
                     /locus_tag="ACZPDN_05055"
     CDS             1055..1318
                     /locus_tag="ACZPDN_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298022.1"
                     /translation="MAKYITRTIIEYDTEVRFVKDGQLDIYKLDGVVGKKEAKKLLIS
                     ILGSKDILIVSCTKKEASSAVYKMLEKDFIAMATKESTCYPPQ"
     gene            complement(1697..2791)
                     /locus_tag="ACZPDN_05060"
     CDS             complement(1697..2791)
                     /locus_tag="ACZPDN_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017687.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HIR1298023.1"
                     /translation="MNLQKYNHFISDDKIHYSGNLLLSKMDKNGNKPETYISTSNRSA
                     GKTTWFGGYILNKFLTKNELFCILMRKKYQLEKAVSFMAYFPSALSVYYPDLEMKEEV
                     GIKGVFNNIYIRLRGKENEWFLCGYSTSLNSSDDIRNFSNVFNNVTRIWFDEFQPESG
                     DYVKDEVKRVFSIHTSLARGGGSQSRYLPLILTGNLIDVNNPYYEHFGINRDINIETN
                     FYRGNGFVLEQGFNKSAADAHSNSTFNQSFSESDYSKLLTKKEYLKDDNTMILKTPNL
                     KGDYLFTIKYYDKYFSIRYLYGLAFYYVNETADLTYNFALAARKEDLSDDCIFDGNNR
                     FKKRMKKIYHNNMVRFSSYKAREAFLEFIK"
     gene            2877..3215
                     /locus_tag="ACZPDN_05065"
     CDS             2877..3215
                     /locus_tag="ACZPDN_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1298024.1"
                     /translation="MHSIGVALLFNLTDLVTGLIAAVKAKELRSTKLRDGIFKKIGFL
                     ICYFLALMIDTYGGEVGFVLEVKLLPIVLGFVCLTEVVSIIENISKITDILPEKLLSI
                     FHISKEDNNG"
     gene            3208..3390
                     /locus_tag="ACZPDN_05070"
     CDS             3208..3390
                     /locus_tag="ACZPDN_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019836.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298025.1"
                     /translation="MADTIFVRIPETIALAIEVINGAYGNGEDRKKALKRAGYDYTKI
                     QNCVNNLLPIWNKYKE"
     gene            3396..4052
                     /locus_tag="ACZPDN_05075"
     CDS             3396..4052
                     /locus_tag="ACZPDN_05075"
                     /inference="COORDINATES: protein motif:HMM:NF037025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HIR1298026.1"
                     /translation="MPDERKISPYVVSAMCGCWAWESGMNPGIWESLIPTTWDHEYQY
                     DGIGGFGLGQWTNVGTPYGRCYNLHTWVTSQGYSDGDLYGQLNFVLHENYWTAANSVM
                     GYNNLSEFLSSTSTNLPLLVEEFLACWEGVPGNKLTERIAYAQNYYQFIYDNKSASPS
                     SWKQTSGNFYQDPTGSNAHANVMLVYWWAGGVEPEPPGPTPGNNGKGMPLWFFMRRII
                     "
     gene            4070..4366
                     /locus_tag="ACZPDN_05080"
     CDS             4070..4366
                     /locus_tag="ACZPDN_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298027.1"
                     /translation="MAVLSKEDLIAKLNASFGENLSDDNISLLEDVSDTIDSFSDMED
                     WKTKYEENDASWRKRYKERFEGKEDDIPGSEPEIEHYKSPTKFEDLFTVESEVK"
     gene            4369..5784
                     /locus_tag="ACZPDN_05085"
     CDS             4369..5784
                     /locus_tag="ACZPDN_05085"
                     /inference="COORDINATES: protein motif:HMM:NF052097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR1298028.1"
                     /translation="MAKRIAQNSLNASTIDILNVIRQNASYDYQQSVPVVKKATQIPQ
                     VGEIICGTPTLANQFLNALVNRIALVRAQSVTFNNPYSRLKKGYLEFGETVEDIFVSI
                     AKVVDYDPDKGEDRELKRSLPDVGSQFHIMNWRVMYPVTIQDEDLRRAFLSEQGVTDL
                     IAKIEESVYTAAEYDEFLLFKYLIIKGVTKGQMYPVAVDDSNMSNYATAFRSKSNAIT
                     FPKTTYNAASVRNNTPRERQVIFMDSDFNAKYDVEVLAAAFNMDKATFMGSLFLIDDF
                     TTFDNERFSVIRNYSDGIAEVTTEELALMADVKAVLLDENWFQCYDNMNKFTEKYVAS
                     GLYWNYFYHTWKTISSSRFANAIVFVANTAKTSLPASITFKVVDKSVSETATVLCLEP
                     QVDGATLEPHNVQLVQTEQATKAGVGVQKYGALLIPASAVATTLELSATVNGTTYKAK
                     PSATISASAEVGDTVTLDKIG"
     gene            5878..7467
                     /locus_tag="ACZPDN_05090"
     CDS             5878..7467
                     /locus_tag="ACZPDN_05090"
                     /inference="COORDINATES: protein motif:HMM:NF044352.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298029.1"
                     /translation="MYITPNSTIELFSDIGLSGNYDNALYFSSTNEKDSYFSNIRKIA
                     TLTNFSYVSQQKGIIRVGTPISNLLSAGYLRYKNTSYENKWFYAFITGIEYRSNGMTE
                     IHFELDYLTTWMGAFQLKQCFVERQHVTDDSIGVNILDEGINFGEHVIEGIHDYTLTG
                     TSSFNPIVIVTAAESGGSGGGIAGGVYSGCAISVFVTAESANNYINDLIDKNKADNIV
                     KIYSLPAKYVVPGGTPIEDRYKETHTNNKPYNTLDGYVPKNNKLFCYPYKYAEVSNGE
                     GDKKDYKYECFNTVPGNASSGTYSFTEQAAFGASTQALFMPINYKVQSMSGNGQLEID
                     ERVSLSSFPLCAYNIDTYRAYTAQQNTSAPNSLFNSFTKGAISGGAAGAGGGILGAIG
                     GAIMGGISNSIGKVVDLLTVNTVPVEMGTRNQGTQESDFLLATKQKGFRIYEKCITKA
                     YAKVIDDYFSAFGYAVRRTATPNMNARPHWTYVKTTDCIVEGNLPSDDARKIENIFNS
                     GCRFWKKHTEIGNYDLDNNPS"
     gene            7480..8361
                     /locus_tag="ACZPDN_05095"
     CDS             7480..8361
                     /locus_tag="ACZPDN_05095"
                     /inference="COORDINATES: protein motif:HMM:NF017190.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298030.1"
                     /translation="MSKRKSYFSDSLNLNMRSYGQYLSILRQISISMFEWKNIPSTID
                     SRYIEQALFYNAGAVYFNDEVVGNLALDVVCNGNFNVYGESVRRVAYSKYNNYRKSLY
                     DTDSVIIWNNMDRTPTFPVIELFAQRLYNLDRIIDVNANAQKTPVLLKCDQKLRLTLL
                     NAFKEMDGNSPVIFADNSFDENSVICLKTDAPFVCDKIYDLKTNLWNEALTYLGIPSA
                     NVMKKERLIKDEVLRGLGGTLANRYSRLSERQHAVEKINTMFGTNIEVTIRDEIDELG
                     QVDLGLDAPTLGGEDNE"
     gene            8354..9277
                     /locus_tag="ACZPDN_05100"
     CDS             8354..9277
                     /locus_tag="ACZPDN_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298031.1"
                     /translation="MSKYTTEVRYICEQKAGLQESVGFNNINSVLDKSWDKIFTTNWE
                     IFDESYRKILCEKILRSYYTREICAETVGLWQLWLDSTLCEIMPMYNQLYKTTIYEFN
                     PLYNTDMTTTFTKTVTGNDSKTTTGNNSKWNDVSNYNKNTKTDDYTVKDSSKTESTSK
                     GNTNSESSNNDTSAETNKFNDTPQGDVNGIESGNYLTDIRMISRTGTTTNSSDENSTS
                     SLNGTYTNENLNKGTTVNEGTSNSNTSESGTTNASETGTSETTETWTEKVMGKNNSEN
                     YGQLLVEFRKSIINIDKMIIDELKPLFMQLW"
     gene            9291..9533
                     /locus_tag="ACZPDN_05105"
     CDS             9291..9533
                     /locus_tag="ACZPDN_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298032.1"
                     /translation="MDKVFIPCSAKILPVSYNDSLSYYEQLCKLTNKMNEIVEFINGN
                     LSEVIQNYIDKKFNDLMINAIYDEATETIILKNETK"
     gene            9546..11477
                     /locus_tag="ACZPDN_05110"
     CDS             9546..11477
                     /locus_tag="ACZPDN_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HIR1298033.1"
                     /translation="MSDVSKFNILDKIVNAKDTEGRAEAIARYNQLLTKMEKEFEEVD
                     NNFLQVDNKFTAVNNAVSKINSKISNFVNVVTDFGADNTAKTDCTEALKKAFAVQDAF
                     IYFPAGNYLISDSIKIKSNTYVYGYRALIQNRDSNNMFINDSDGTVGGYNANSHITID
                     GLWFRGLNMTQTIVAFGHCSDIRIINCDFASNSATHEQQNWHLVEINSCRRVLIENCH
                     FTGTATFKTEMLQLDVATQTTVFPWFGPYDNTPCTNVEISNCNFSHPEKYGYETLGLS
                     DAGIGNHNGANSAPIEYVNIHGCHFNNVKTAFKFEYLRFSIIDNNIAENCMSGFAYLT
                     SHIIDTVKITNNTFYGNVDDYTDKISNTALGRGISIGTLNGQQCSNNIISGNNVIGFA
                     SHGIAVNGEFCDVSNNIIKGNGYTGLYTDYDNYKCNFHDNICDGNARLDQENYDLFVS
                     HTHTTKIIRSGGNDIYNNKASIIRCAVYSTDNLKSRVHDNVYSEFTYPTTFNKLNVYG
                     NTKFDGNPNYRFDNAVIPSPAGGQWYTPVNFTTDHTCYALINFQVIIPANFVGTFDIR
                     IIDTSSKTTLAFNTIDTSHASSTTHTGANLTTCVKILNGHTISGELFFVYSDKAVENV
                     DAQIIMLELPVPLENTDTV"
     gene            complement(11593..11793)
                     /locus_tag="ACZPDN_05115"
     CDS             complement(11593..11793)
                     /locus_tag="ACZPDN_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298034.1"
                     /translation="MTVQDLLYACGNVDKKTLITVIQATGKELVSQKKVSLFYELPES
                     ESILKKEVDYFKMFSHAIVILL"
     gene            complement(11790..11978)
                     /locus_tag="ACZPDN_05120"
     CDS             complement(11790..11978)
                     /locus_tag="ACZPDN_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298035.1"
                     /translation="MTVKELYIKCLNFGDNSWVNLFKGNEKVGSMYMRELVKRYENAE
                     VEWFEFCKNNTYDMEVKL"
BASE COUNT         4147 a         1993 c         2343 g         3672 t
ORIGIN      
        1 attagaaaac tacaacaaac aatggattga atacgctgaa aattgtaatg tatcgttcaa
       61 agcatacacg aaaaaaggag atttacaaat tttaggtatc gcggattttg atggatttta
      121 taaacgattt acaactctcg gcgcaaagaa atacgcgtac gatgacgaaa acgatcaatt
      181 acacattaca atagcaggag tgcctaaaaa actaggagca aagcttttag gtaaaatcga
      241 aaattttaaa gtcggtatgc attttatggt gggagccgat ggaacgctgg aagatcggca
      301 agcatggaaa aaacgattgt tatacaatga tactgacaac ttcgatctca ctattgatgg
      361 aaacattcta cacatcggaa catacattgc aatggaaaga acttcgtatg aattatcaat
      421 tactgatgag tacgaagaac ttatttcttc tttgaaaaat gatgaaatat atgaaaagga
      481 tgatatatgg ggttgacaaa catataaaaa cgtgctaaga tatatatgta ccggatagag
      541 gggttcggat aataaaatac agattgtcac gggtgaaacc gctggatttt atttacgaat
      601 gataaacggt gtcggaactt ctctattgca ggtactacaa aatataaata aaaggagaat
      661 aaaaacatga gtgcaaaagt aattaaaaca agcgaaggta tgagtgcaag aaaagttttg
      721 tcttatacct caagaaatga tgccattccc atgaaagagt tggctaaggg aacgatcatt
      781 ccttttaaag gttatattga acaggaaatt ataaatgaaa ttactggaga ggtgttcaat
      841 tctcttctca tcatttctga gcctgatgaa tcaggttgcg atgcattata cgcgaccaga
      901 tcggaaagcg taatgcgttc tttatcagat attattgata cgctcacaga tatgggagac
      961 actgacccgt tctcagttaa agttgataaa ttaaaatcta aaaatgggcg cgagtttatt
     1021 actcttagcc ttgctgacta aaggagaata gaatatggca aaatatatta caagaaccat
     1081 tattgaatat gacacagaag ttagatttgt aaaggatggg cagcttgata tatataaatt
     1141 ggacggagta gtaggaaaaa aagaagcaaa gaagctcctt atttctatct taggaagtaa
     1201 agatattctt attgtttctt gcacaaagaa agaagcttct tcggcagtat acaaaatgct
     1261 tgagaaagat tttattgcaa tggcaacaaa agaaagcacc tgttatccgc cccagtaata
     1321 aaccccttat ggttagggga accccaattc tttcctcttt tccttatata gccgccggat
     1381 taaaactccg gcggttttta ttgacaattc tttaggattg tgatataata aataagggga
     1441 aggggtgatt ccattgcact agtgagaaca aataaacttt ataatagaac ttaagagcaa
     1501 atttttcatg ataatagaaa taacgacaaa taaaggataa tatccgaagt aaagattatt
     1561 tgttagcagt ctataatcat gaaaaagttt gtatatgagt aactgtagat tcatttttgt
     1621 atctacccac ggtgcgctat ctaaaaacag tcattatcca aacaaattac cccgtcttct
     1681 ctcggcgggg taatttttat ttaataaatt cgagaaaagc ttctcgcgcc ttatagcttg
     1741 aaaatcgaac catattgtta tgatatattt ttttcattcg ttttttaaaa cgattatttc
     1801 cgtcaaaaat acaatcatcc gataagtcct cttttcttgc ggcaagtgca aagttatatg
     1861 tcaagtcagc ggtttcatta acatagtaaa acgccagacc atacaagtaa cgaattgaga
     1921 aatatttatc ataatactta atagtaaata agtaatcacc ttttaaatta ggagttttta
     1981 aaatcatagt gttatcgtct tttaaatatt ctttttttgt taatagttta ctgtaatcag
     2041 attctgaaaa agattgatta aatgtgctat ttgaatgagc atctgcggca cttttattaa
     2101 acccttgttc aagaacaaag ccatttcctc tataaaaatt ggtttcaata tttatatcgc
     2161 ggtttattcc aaagtgttca taatatgggt tatttacatc aattaagttt ccggttaata
     2221 taagaggtaa atatctactc tgcgagccac cgccccttgc cagtgatgta tgaatagaaa
     2281 aaactcgctt cacctcgtct ttgacataat cgccgctttc tggctgaaat tcgtcaaacc
     2341 atattcttgt aacattatta aaaacatttg aaaaatttct tatatcatcg cttgaattga
     2401 gtgaagtgct atatccacat aggaaccatt cgttttcttt tccccgaagt ctaatgtaga
     2461 tattgttaaa tactcctttg attcctactt cttctttcat ctctaaatca ggatagtaaa
     2521 ctgataacgc gctgggaaaa tatgccataa aagaaactgc tttttcaagc tgatattttt
     2581 tcctcattaa aatacaaaac aattcatttt ttgttaaaaa tttatttaaa atgtatcccc
     2641 caaaccatgt agttttgcca gcactacggt tcgatgttga aatatatgtt tctggcttat
     2701 ttccgttttt atccattttt gataatagta gatttccact ataatgaatt ttatcgtctg
     2761 atataaaatg attatacttt tgtaaattca tagtacgcac tcctttacat attattttat
     2821 ttatgctata tttatattgt aacacatata caattaaaag taaaggaggt gaatgtatgc
     2881 atagtatcgg cgtggcatta ctctttaatt taacagatct agttactggt ttaattgcgg
     2941 cagttaaagc aaaggagttg cgatcaacta aactgcgtga tggaattttt aaaaaaatcg
     3001 gtttcctaat ctgttatttt ttagcgttaa tgattgatac atacgggggt gaagtcggtt
     3061 ttgtattaga agtaaaatta ctcccgattg tcttaggttt tgtatgcctt acagaagttg
     3121 tttcgatcat cgaaaatatt tctaaaatta ctgacattct acccgaaaaa cttttgtcaa
     3181 tttttcacat ctcaaaggag gataacaatg gctgatacaa tttttgttag gattccagaa
     3241 acgattgctc ttgcaatcga ggttatcaac ggagcatatg gaaacggaga agatagaaaa
     3301 aaggcgttaa aaagagccgg atatgattat acaaagattc agaattgtgt gaataatctg
     3361 cttcccatct ggaacaaata caaggagtga tagatatgcc agacgaaaga aaaatcagtc
     3421 cttacgtcgt ttccgccatg tgcggttgct gggcgtggga atccggcatg aatccgggaa
     3481 tatgggagtc tctaattcct acaacgtggg atcacgaata ccagtatgac ggtataggtg
     3541 gttttggatt aggacagtgg actaacgttg gaaccccgta tggccgttgt tataatctgc
     3601 atacctgggt cactagtcag ggatattctg acggagattt atatgggcag ttaaattttg
     3661 tactgcacga aaattactgg acagcggcaa attcagtgat gggatataac aatttatctg
     3721 aatttttgtc atcaacaagc acgaatttac cattactggt agaagaattt ctggcatgct
     3781 gggaaggtgt tcccggaaat aaattgactg aacgtattgc atacgcgcaa aattattatc
     3841 agttcattta cgataataaa tctgcgagtc cctcttcctg gaagcagacc tccggcaatt
     3901 tctatcaaga ccccaccgga agcaatgcgc atgcaaacgt aatgcttgta tactggtggg
     3961 ccggaggagt ggagcccgag ccgcccgggc caacacctgg caataacgga aaaggcatgc
     4021 cgttgtggtt tttcatgaga agaattattt aagaaaggag ttaataaaaa tggcagtatt
     4081 atcaaaagaa gatttaatcg ccaaacttaa tgcaagcttt ggggaaaatt taagtgacga
     4141 taatatttca ttgcttgagg atgtctcaga tactattgat tcattctcag atatggaaga
     4201 ttggaaaaca aaatacgaag aaaacgacgc ttcttggcgc aaacgttaca aggaacggtt
     4261 tgagggaaaa gaagatgata tccctgggag cgaacccgaa attgagcatt ataaatcacc
     4321 gacaaaattt gaggatttat ttactgtaga aagtgaggtc aaataactat ggctaagaga
     4381 attgcacaga atagtcttaa tgcgagcacg attgacattt taaacgttat caggcagaat
     4441 gccagttatg attatcagca gagcgttcct gtagtaaaaa aagcaacaca gattcctcag
     4501 gtaggagaaa ttatctgcgg cactccgacg ttagcaaatc agtttttaaa tgccctggta
     4561 aaccgtattg cattagtgcg ggcgcagagc gtaacattta acaacccgta ttcacgactg
     4621 aaaaaaggat acctggagtt cggagaaacg gtcgaagata tttttgtaag cattgcaaaa
     4681 gtggttgatt atgacccgga caaaggagaa gatagagagc ttaagcgttc ccttcctgat
     4741 gtgggcagcc agtttcatat catgaactgg cgtgtgatgt acccggtgac cattcaggac
     4801 gaagatttga gaagagcttt tttaagcgaa cagggagtaa ctgacttaat cgctaaaatt
     4861 gaagaatctg tatatactgc cgctgagtat gatgaatttt tgctgtttaa atatttgatt
     4921 atcaagggag taacaaaagg gcagatgtac cctgtagcgg ttgatgattc taacatgtca
     4981 aattacgcaa ccgctttccg ttcgaaatct aatgcgatca cgttcccaaa aacaacttac
     5041 aacgcggcat ccgttagaaa taatactcct cgcgaaagac aggttatttt tatggattct
     5101 gatttcaacg caaagtatga cgttgaggtt ttagcagcgg cgtttaatat ggataaagct
     5161 acttttatgg gaagcctttt cctgattgat gattttacaa cgtttgacaa tgaacgattc
     5221 agcgttatcc gcaattactc cgacggcatt gcagaagtaa cgactgagga acttgctctt
     5281 atggcagatg taaaagcagt cctgttagat gaaaattggt ttcagtgcta tgataatatg
     5341 aataaattta ctgaaaaata tgttgcgtcg gggctgtatt ggaattactt ctatcacaca
     5401 tggaaaacga ttagttcttc ccggtttgct aacgccattg tgtttgttgc aaataccgct
     5461 aaaactagtc ttcccgctag tatcacgttt aaggttgtcg ataaatccgt ttccgaaact
     5521 gctacagttc tttgtttgga accgcaggtg gacggagcta cattagagcc gcataatgtt
     5581 cagttagttc agacagaaca ggcaacaaaa gcgggtgtag gggtgcagaa atacggagct
     5641 ttgctgattc ctgcaagtgc tgtagcaact actcttgaat taagcgcaac ggtaaatgga
     5701 actacttata aagccaaacc ttctgctaca attagtgcaa gtgccgaggt tggagatact
     5761 gttacactcg ataaaattgg ataataacca gcggggatat tatccccgct aattttataa
     5821 aaggaaatat taaatgaaag atagaaatat aaatagtata tagaaaggtg tgataatatg
     5881 tatattactc ctaacagtac aatagaatta ttttcagata ttggtttatc tggtaattat
     5941 gataacgcat tatattttag ttcaacaaac gaaaaagatt catatttttc aaatatcaga
     6001 aaaatagcaa ctttgaccaa cttttcgtat gtatctcagc aaaagggcat tatcagagtc
     6061 ggaactccga tttccaatct cctttcagca ggatatttac gatacaaaaa tacatcgtat
     6121 gaaaataaat ggttttatgc atttatcact ggaattgaat atcgttcgaa cggaatgacg
     6181 gaaatccatt ttgagctcga ctacctcacg acgtggatgg gagcttttca gttaaagcaa
     6241 tgctttgtcg aacgacagca tgtgacagat gattcaatcg gagttaatat tcttgatgag
     6301 ggaatcaact ttggagagca tgttattgaa ggtattcatg attataccct caccggaaca
     6361 tcatcgttta atcctatcgt tatagtgaca gcggcagaat cgggcggttc tggcggaggt
     6421 atcgctggtg gagtttatag cggatgcgct atttctgttt ttgtaacggc agaatccgca
     6481 aataattata tcaatgatct aattgataaa aataaagcag acaatattgt taaaatatat
     6541 tcacttccgg caaaatacgt tgttcctggc ggaactccga ttgaggacag atacaaagaa
     6601 acacatacaa ataataaacc ctataacact cttgatggtt acgttcccaa aaataataaa
     6661 ttattctgct atccgtacaa atacgccgaa gtaagcaatg gagagggtga caaaaaagat
     6721 tataagtatg agtgcttcaa tactgtaccc ggaaacgcaa gtagcggaac atatagtttc
     6781 acagaacagg cggcctttgg ggcatcaaca caggctcttt ttatgccaat caattataag
     6841 gttcagtcta tgtcaggaaa tgggcagttg gagattgacg aacgagttag cttgtcaagc
     6901 ttccctttat gcgcttacaa cattgataca taccgcgcct atactgcaca gcaaaataca
     6961 tccgcaccga acagcctgtt caacagtttc acgaagggcg caatcagtgg cggagccgca
     7021 ggagcggggg gaggtatcct cggtgcaatc ggcggcgcaa ttatgggtgg cattagtaat
     7081 agcataggaa aagtagttga tttattaact gttaatactg tacctgttga aatgggaaca
     7141 agaaatcagg gaacacagga aagtgatttc ctgcttgcca caaagcagaa aggtttcaga
     7201 atctatgaaa aatgtattac gaaagcatac gcgaaagtaa ttgatgatta tttttcagct
     7261 tttggatatg ccgtaaggcg aactgctact cccaacatga atgcaaggcc gcattggacg
     7321 tatgtaaaaa ctacagattg catagtggag ggaaatttac cgtctgacga tgcaagaaaa
     7381 atcgaaaata ttttcaactc tggttgtagg ttctggaaaa aacacacgga aattggaaac
     7441 tatgaccttg ataacaaccc gtcgtaagga ggtgatattt tgagcaaaag gaaaagttac
     7501 tttagtgact cattaaatct taatatgcga tcatacgggc agtatctttc tattctacgg
     7561 cagatttcta ttagtatgtt tgaatggaag aacatacctt ccactattga tagtcgctat
     7621 attgaacaag cgttgtttta taacgcaggt gcagtatatt tcaatgacga agtggttgga
     7681 aaccttgcgc tggatgtagt atgcaacgga aattttaacg tttatggtga atctgtcaga
     7741 cgggtagctt attcaaaata taataattat cgtaaatcct tatatgatac agacagtgtt
     7801 attatatgga ataacatgga cagaactccg acttttccgg ttattgaatt atttgcgcag
     7861 agactttaca atttagacag aatcattgat gtgaatgcca acgctcaaaa aacacctgtt
     7921 ttgctaaaat gcgatcaaaa actaagatta acactactga acgcgtttaa agagatggat
     7981 ggaaatagtc ctgtaatatt tgctgacaat tcctttgatg aaaattctgt tatttgttta
     8041 aaaacagacg caccttttgt atgcgacaaa atttatgatt taaaaacaaa cttgtggaac
     8101 gaagctctta catacttagg tattccatct gcaaacgtta tgaaaaaaga acgcttaatt
     8161 aaagacgagg ttctaagagg tcttggcgga acgctcgcta atagatattc aagattatct
     8221 gagcgacaac atgcggtaga aaaaatcaat actatgttcg ggacaaatat cgaggtgact
     8281 ataagagatg aaattgacga actcggacaa gtagaccttg gtttagatgc tcctacttta
     8341 ggaggtgaag acaatgagta agtacacaac agaagtaaga tatatttgtg agcaaaaagc
     8401 aggactacag gaaagcgtcg gattcaataa tatcaattct gtacttgaca agtcttggga
     8461 taaaatcttc acaactaact gggaaatttt cgatgaaagc tatagaaaga ttctctgcga
     8521 aaaaatcttg agatcttatt atacacgaga aatttgtgca gaaaccgttg gtttatggca
     8581 gttgtggctt gactcaacgt tgtgcgaaat tatgccaatg tacaaccaat tgtataaaac
     8641 aactatttat gaatttaatc ccctgtacaa cacagatatg actactacat tcacaaaaac
     8701 agtaacggga aatgatagca aaacaacaac gggaaataac agcaaatgga acgatgtgtc
     8761 aaattataac aaaaatacta aaacagatga ttacactgtt aaagattcat caaaaacaga
     8821 gagcaccagc aaaggaaata ctaattcaga aagtagtaat aatgatacat ctgctgaaac
     8881 aaataaattc aatgatacac cgcaaggaga tgttaatgga attgaatccg gaaattattt
     8941 gactgatatt cgaatgattt cacgaactgg cacaacaact aattcatcag atgaaaattc
     9001 tacaagttcg ctaaatggaa catatacgaa tgaaaatttg aataagggta ctactgtaaa
     9061 tgagggaact agcaattcaa atacaagcga aagtggaaca acaaacgctt ctgaaactgg
     9121 tacatcagaa acaaccgaaa catggacgga aaaagtaatg ggaaagaata acagcgaaaa
     9181 ctatggacaa ttattagttg aatttagaaa gtcaattatc aatattgata aaatgataat
     9241 tgatgaattg aaaccattat ttatgcagtt atggtaggag gtacaaatat atggataaag
     9301 ttttcattcc ttgtagcgca aaaatattac cggtaagtta taatgatagt ttaagttatt
     9361 atgaacagct gtgcaaactt acaaataaaa tgaatgaaat tgttgaattt attaacggta
     9421 acttaagtga agtaattcag aactacattg ataaaaagtt taatgattta atgataaatg
     9481 ctatttacga cgaagcaacc gaaactatta ttttgaagaa cgaaacaaaa taggaggtat
     9541 ggctaatgag cgatgtaagt aaatttaaca ttttagataa gatagttaat gcaaaagata
     9601 ccgagggcag agcagaagca atcgcacggt ataatcaact tttaactaaa atggaaaaag
     9661 aattcgaaga agtcgacaat aatttcttgc aagttgataa taaatttaca gctgttaata
     9721 atgctgttag taaaataaat agcaaaatat cgaattttgt taatgttgtt actgatttcg
     9781 gagcagataa tacagctaaa actgattgca cggaagctct gaaaaaagct ttcgctgtgc
     9841 aagacgcttt tatttatttt ccggcaggta attatcttat cagtgatagc attaaaatta
     9901 aatctaatac ctatgtatat ggttatcggg cactaattca gaacagagat agcaataata
     9961 tgttcatcaa tgattctgat ggaaccgtgg gaggttataa tgctaatagt catatcacta
    10021 ttgatggatt gtggtttaga ggtttgaata tgacgcaaac gattgttgcg tttggtcatt
    10081 gtagtgatat tcgaattatc aactgtgatt ttgctagcaa ctcagctact catgaacagc
    10141 agaattggca tctggtagaa atcaacagtt gtagaagggt gctgattgag aattgccatt
    10201 ttacaggaac cgctaccttc aagactgaaa tgttgcagtt ggacgttgct actcaaacaa
    10261 cggtttttcc gtggttcggg ccgtatgata atacaccgtg tactaatgta gaaattagta
    10321 attgtaattt ttcacaccca gagaagtatg gatatgagac actaggttta agtgatgcag
    10381 gaattggtaa tcataatgga gctaattcgg ctccgattga atatgtaaat attcatggat
    10441 gccatttcaa taatgtaaaa acagcattta aatttgagta tttacgtttt agtattattg
    10501 ataataatat tgctgaaaat tgtatgagtg gttttgcata tcttacctcc catattattg
    10561 atactgtaaa aattacaaat aacactttct acggcaatgt tgatgattat accgacaaaa
    10621 taagcaatac agcccttgga cgtggtatct cgattggaac attgaatgga caacagtgta
    10681 gcaataatat tatcagtgga aataatgtta ttggattcgc ttcgcatgga atcgctgtaa
    10741 atggtgaatt ttgcgacgta agtaataata tcatcaaagg taatggatat accggattgt
    10801 atactgatta tgataattat aaatgtaatt ttcatgataa tatctgcgat ggtaacgcga
    10861 gattagacca ggaaaactac gatctttttg tttctcacac tcatactact aaaataattc
    10921 gaagtggtgg aaacgatatt tataataaca aggcgtctat tatcagatgc gcggtttaca
    10981 gtactgataa tttgaaaagt agggtacatg ataatgtata cagtgagttt acatatccta
    11041 ctacttttaa taagcttaat gtgtatggaa atactaaatt cgatggtaat ccaaattata
    11101 gattcgataa tgctgttatt ccctctcctg cgggggggca atggtatact ccagtaaatt
    11161 ttactacaga tcatacttgt tatgctttaa ttaactttca ggtaattata cccgctaatt
    11221 ttgtaggaac ttttgacatt agaattatag atactagttc taagactact ctagctttta
    11281 acacaatcga cacgtcacac gcaagtagca ctactcatac tggagctaat cttactacat
    11341 gcgtaaaaat tctaaacggg cacactattt ctggcgagtt attttttgtt tatagtgaca
    11401 aagcggttga aaatgtagat gctcaaatta ttatgcttga attacccgtg ccacttgaaa
    11461 acactgatac agtataagga gggctaaagc cctcctttat tatgcaaata catatattat
    11521 taacattaag attacaaata aatctaatat aaaacatgtg atcaagaaat ctttcattgt
    11581 ttaatcatct ccttatagca atataacaat agcatgagaa aacatcttaa agtaatcaac
    11641 ttcctttttt aatatgcttt ctgactccgg taattcgtaa aaaagtgata cctttttctg
    11701 tgaaacaagc tcttttcctg ttgcctgtat taccgtaata agcgttttct tatcgacgtt
    11761 gccgcatgca tataataaat cttgcactgt catagtttta cctccatatc ataagtatta
    11821 ttcttacaaa attcaaacca ttctacttca gcgttttcat aacgttttac taattctctc
    11881 atatacatag aaccaacctt ttcattacct ttaaataaat taacccatga attatcacca
    11941 aaattaaggc atttaatata caattctttt actgtcatct ttatccctcc tttagaatac
    12001 tatcatttta caacttgaat catcatcttc gattaaccca gcaacctcgt tgtttctcat
    12061 ttctttatcc tccttattga aattttctca tttctttgat gctttaagta taccacatgt
    12121 gcacaccttt gtcaaacctt ttttgcaaat tatca
//
LOCUS       DBMDWL010000053        12114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1788_length_12114_cov_2.654541, whole genome shotgun sequence.
ACCESSION   DBMDWL010000053 DBMDWL010000000
VERSION     DBMDWL010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1788_length_12114_cov_2.654541"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2176
                     /locus_tag="ACZPDN_05125"
     CDS             <1..2176
                     /locus_tag="ACZPDN_05125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1298036.1"
                     /translation="MYLQEKSQDLKVAKRIYFHLVENEEDIDFPFAFLATYATKDKEN
                     RIVHMSLKHALVEYKNDQEQLLNLLSCLNVVAQKNTLIAQYMETGDLFHPIKLTSKEA
                     YSLLKSVPDIEACGIKCRVPNWWKKKYSSVKINVNIGDTKPSMFGFDSILSLQPSLIV
                     NGHALTKKEISELLKMEEGLAWLKGQWVEINHNKLQQLLEQMEQYDGTITLKEALTKT
                     YMSNDDIDVDMGIQISNGKWLRDILSKLKNPSKIKSKAQPKYLNATLRPYQKSGYNWL
                     NQMNDLGFGACLADDMGLGKTLQVISFLEKMYEKKKDGHVLLIVPASLLGNWSKEIDR
                     FAHKMPYYILHGKNNIVHEDTFITITTYGMALRNVFLQERVWDCLILDEAQAIKNPAT
                     KQTRAIKKIPSHMRIAMTGTPIENDLSNLWSLFDFLNKGLLGSESEFKEYAKKVQAYP
                     EYMTKLKMMVSPFILRRLKTDKTIISDLPDKMEITEHVDLSKRQVVLYRKEVDKAEQK
                     ILESTGLQRRGIVIALLTKLKQICNHPDQFIHDTNYNPKDSGKYAMLKELCETIYEKR
                     ERVLVFTQYKEMCEPLSQYLESIFHKKGYIIHGGIPAKKRTEIVEKFNDENTYVPYIV
                     LSLKAAGTGLNLTAANHVIHFDRWWNPAVENQASDRAYRIGQTKEVFVHKLVSNGTIE
                     EKIDKLIESKQELANSILASSQEKWITELDTNELMDLLRLDV"
     gene            2180..2911
                     /locus_tag="ACZPDN_05130"
     CDS             2180..2911
                     /locus_tag="ACZPDN_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR1298037.1"
                     /translation="MAKYWTASMQYSQMSVEELQKKAQESVKNAKNKGKEMHPIIVDG
                     RQIAKSWWGMAWCKNIEQYADYASRIERGKRYVRSGAVVDLQIVKGKVKARVQGTRKT
                     PYKVEINISPLKEEICEDIIEKCSSQIQNLQELVNGQFPESLKEIFLSENGLFPSQRE
                     ISFNCSCPDWALMCKHVAAVLYGIGTKLDENPFLFFELRGIDVNKLVDVTIKDHVDSL
                     LEHANMPPTDRVMDDSCIETLFGLK"
     gene            complement(4068..4748)
                     /locus_tag="ACZPDN_05135"
     CDS             complement(4068..4748)
                     /locus_tag="ACZPDN_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298038.1"
                     /translation="MSKNNYKPKMPDLMEAIFDACYLIFDLIAGILFFVYSKGNPLFI
                     LYGVLTLTLCGGDAFHLVPRIKRAVYGTNDKIKRQLGIGLQVSSITMTVFYIILLFIW
                     KLTFPTLTAPLWIEAMIWILAIIRIVVCFLPQNNWTSEEGNRKLSLIRNAVFAVTGIG
                     VILLYAISGNMYDLHLNRMVAAIIISFGCYIPVTLWSKKKPMIGLLMIPKTCAYMWVI
                     VMGLQLLF"
     gene            complement(4741..5169)
                     /locus_tag="ACZPDN_05140"
     CDS             complement(4741..5169)
                     /locus_tag="ACZPDN_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1298039.1"
                     /translation="MHFWDEHKTITRYYEMKMAGVCEKYDLRQMEYDILMFLYNNPKY
                     NTAADIVRIRKSTKSHVSTSLKVLEDRGLIERRVDPANKKRVTIHLLQLANEVIEDGK
                     WAQKEFAQDMFDGLTEEEIKVFMNVFQKVYDNAASMISNE"
     gene            5297..7081
                     /locus_tag="ACZPDN_05145"
     CDS             5297..7081
                     /locus_tag="ACZPDN_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024878.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR1298040.1"
                     /translation="MRTQKYNLQGIKVFFYILMIAILGWFCFMQLFGANEQEHDIQSH
                     SIVYTQTFYWEKEDGSKQKINVPGKYEVKPGHTMVIRTTLPKDYNETSFAIRSSLQDV
                     KFYVDNTLRKEYSTKDTRLAGKNSASRYIFCPTTYKDAGKTLRIELTTYTSNYSGVVN
                     EIYCGSEIDIWQHIYSDYKMSTFIAFFILFAGITSVLFGSALKFTYHTNFRTEYLGWC
                     MIMGAIWMLGESKIRQILVPNASSLASLCFVMIMLSPLPILFYADSIQNGKHRKLYKY
                     IGCIALLNFAVSSILYLAKVKDYIETLPVAQCILICVFILVFIHLCQYIRKYSHSRSD
                     YFLLFGLFLVLVCIAIESISVYFVAMISGIFIGIGMIVLLLTNIVRTIHRIQLIEQTR
                     HQKELDIEKKENKKITLQMMQSLSTTIEAKDEYTRGHSRRVAQYAALIAKNLGWTDQE
                     IENIKSCAYLHDIGKIGIPDQILNKPGQLTEDEFNLIKQHPIIGQDILKDITIIPHLG
                     EVTRSHHEHYDGTGYPDGLKGNEIPIQARIITLADSYDAMNSKRIYRNALSFKQIKEE
                     IEKMQVHNLILKSPKSFLNSWTMNLYLT"
     gene            7054..8079
                     /locus_tag="ACZPDN_05150"
     CDS             7054..8079
                     /locus_tag="ACZPDN_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1298041.1"
                     /translation="MDDESLFNLEHEIIPVQENTIDKFISDVVSTIKNQEETKNHDYL
                     TGLPVRSVGQKYIAIAMQKTTGCLIFLDMDNLKKINDVYGHKAGDRALKSLGKLLLDI
                     PLDKITCRMGGDEFLLFLPKVNSEQAEKILAQVIEQFKGIVENDDEIHFATLSAGMLM
                     TTKNDNFEDACSKADKALYYVKQNGKNNYSFYNQIQHKNTSNNTADLKQIAKSLQTSG
                     SYTGALSLDFREFTRHYEYIHQLMVRNHSCCYLVMVTMETVEDTLPYIEKIEEALTHM
                     GEAIHKNIRKVDVCTRYSAMQYLIILSQPTEAEIPNIMTRIFIQYYKLQDSQNFTPTY
                     EYITMKD"
     gene            complement(8111..9364)
                     /locus_tag="ACZPDN_05155"
     CDS             complement(8111..9364)
                     /locus_tag="ACZPDN_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1298042.1"
                     /translation="MNRDVLKQIIIDQKEMYLDNPLISRDYDLEENVNYCFVGIRRMG
                     KSYMMYQQIHNLMNDGISSSQIVYVNFEDERLLEIGVDDLNTILELGIEFSGSKGKPY
                     LFLDEIQNVDGWEKFVRRVADMKYRINITGSNSKMLSKEIASTLGGRFMIVNVFPYSF
                     KEYLSANHIENVRLDQIGTKKRADIVSQYEQYVTYGAFPELVDIKNKRPFLNNIYQTV
                     YLQDIITRNKITNDFAVRLILKKIAESVTKALSFNRLTNIVKSAGISIGKQTVINYVG
                     HMLDSYLIFSLQNYASKKTSPKYYFMDTGLLGLMLLDCKTAQLENLVAVELIRRYGFE
                     NVYFFENNIEVDFYIPSENLAIQVSMQVLEDVDTLERETKAFVKLNQFIPDTKCLLVT
                     NSEETKLNCAGIKIDVVPAWKWLLD"
     gene            complement(9506..10960)
                     /locus_tag="ACZPDN_05160"
     CDS             complement(9506..10960)
                     /locus_tag="ACZPDN_05160"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR1298043.1"
                     /translation="MKQLPTGFPKDFLWGGASAACQMEGAYDVDGRGLSTSDIHEYDK
                     NQDRAHIEKEGGGTLAGIKAAIADKEGYYPKRYGIDFYHTYKEDLAYLKEMGFKAFRT
                     SISWSRIFPNGDELEPNEKGLEFYDHLIDEIIKDGMEPVITISHYDIPLHLVTEYGGF
                     ANRKVIDFFVRYGKVLLERYKGKVKYWIVCNQVNLIPNVQFGCLGIYDDQVAPEKMEE
                     TMYQAVHHQFIGCAKIKEFAKEIDPKAHIGTMIAEGCVYPKTCNPKDIVGAMKRNRVQ
                     EYFFTDTQLRGEYPTYMLRHFEEKGIHIHFEDGDEELLKNNTMDFLALSYYYTNVFNH
                     ETDTGVPFKSEQNPYLKPTPWEWRADPLGFYNCLNQLWDRYQVPLMIAENGLGAEDVV
                     EEDGSIHDEYRIEYLKAHIEQMKEAVLDGVNVFSYLSWGPIDIVSSSSAEMSKRYGYV
                     YVDYDDFGKGTGKRMKKDSFYWYKHVIETNGEEL"
     gene            complement(10962..>12114)
                     /locus_tag="ACZPDN_05165"
     CDS             complement(10962..>12114)
                     /locus_tag="ACZPDN_05165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR1298044.1"
                     /translation="FRTSIAWTRIFPRGDEKEPNEEGLKFYDDLFDEMILDGMIPMVT
                     ISHYEMPLNLSIKYGGWYSKKTLESFKRFVDVIMERYKNKVKYWIAFNQINTLFGEGY
                     NSLSIPYDWTDDFTSACFQGLHYQFVASAYAKKKMDEMNTGMKMGCMVCNGISYPFNC
                     DPNEVMASCKSNQIQYMFLDVLCRGKYPRSLQRYMDENKIKPFEATKEELDLIEKYPC
                     DFLTFSYYGTGVCSSDLKPGERKKNPYVKENEWGWINDPVGLRYVLNEYYDRYQLPIM
                     ITELGSGFDEKPDSEGKIHDDYRIAYHKEHMEQMKEAIYDGVELIGYYPWGPIDIVSC
                     TSSEMTKRYGFVYVDYDDLGNGTGKRIKKDSFDWYKRVIETNGEDLGGK"
BASE COUNT         4239 a         2327 c         1751 g         3797 t
ORIGIN      
        1 aatgtatctt caggaaaaat cccaagattt aaaagtcgca aaacgaatct attttcattt
       61 agtcgaaaac gaagaagata tagactttcc atttgcgttt ttagcgacat atgccacaaa
      121 agacaaagaa aatcgaattg ttcatatgtc actaaaacat gcactcgtcg aatataaaaa
      181 tgatcaagaa caattattga atttattatc ctgcctaaat gtagtagctc aaaagaatac
      241 tttgattgcc caatatatgg aaacaggtga tttatttcat cccatcaaat tgacaagtaa
      301 agaagcctat tccctattaa agagtgtgcc tgacattgag gcttgtggca ttaagtgtag
      361 agtaccaaat tggtggaaga aaaagtattc ttctgtaaaa atcaatgtga atattgggga
      421 taccaaacca tccatgtttg gatttgactc catactatcc ttacaaccat ctctaattgt
      481 taatggtcat gcccttacaa agaaagaaat tagtgaactt ttaaaaatgg aagaaggact
      541 tgcatggtta aaaggtcaat gggtagaaat taatcacaat aagcttcaac aacttcttga
      601 acaaatggaa caatatgatg gaactatcac tttaaaagaa gctttgacaa aaacatatat
      661 gtccaatgat gatattgatg tcgatatggg tatacaaata tcaaatggaa aatggctaag
      721 ggatatttta agcaaattga agaatccttc aaaaatcaaa agcaaagctc aaccaaaata
      781 tttaaacgca acactacgcc cttatcaaaa gagtggatac aattggttaa atcaaatgaa
      841 tgatttaggc tttggcgcat gccttgccga cgatatgggt ctaggaaaaa cacttcaagt
      901 catttctttt ttagagaaga tgtatgaaaa aaagaaagat ggacatgtat tattgattgt
      961 tcctgcatcc ctactgggaa actggtcaaa agaaatagat cgctttgcac ataaaatgcc
     1021 atattatatc cttcacggaa aaaacaatat tgtacatgaa gacacattta tcactataac
     1081 aacctatggg atggcattac gcaatgtatt tttacaagaa agagtatggg actgtttgat
     1141 tttagatgag gcacaagcca ttaaaaatcc ggcaaccaaa caaacacgtg ctattaaaaa
     1201 gattcccagc catatgcgca tcgcaatgac aggtacaccg attgaaaatg acctatccaa
     1261 tttatggtca ctatttgact tcttaaataa aggattatta ggtagtgaat ctgaatttaa
     1321 ggaatacgca aagaaagtac aagcctatcc tgaatatatg acaaaattaa aaatgatggt
     1381 ttccccattc attttaagac gtctaaaaac agataaaaca atcatttcag atttaccaga
     1441 caaaatggaa atcacagaac atgtggattt gtccaaacga caagtcgttt tgtatcgcaa
     1501 ggaagttgat aaagcagaac aaaaaatatt ggaatccact ggacttcaaa gaagaggtat
     1561 tgtgattgcg ttattgacga aattgaaaca aatctgtaat cacccagacc aatttattca
     1621 tgataccaac tacaatccaa aggatagtgg aaaatatgcg atgttgaaag agttgtgtga
     1681 aaccatctat gagaaaaggg aaagagtatt ggtatttaca caatataaag aaatgtgcga
     1741 acccctatcc caatacctag aatctatctt ccataagaag ggatatatta ttcatggtgg
     1801 tattccagct aaaaagcgta ctgaaatcgt tgaaaaattt aatgacgaaa acacatacgt
     1861 tccatatatc gtattatcgt taaaagcagc cggaaccgga ctcaatctaa cagctgccaa
     1921 ccacgtcatt cactttgacc gttggtggaa cccagccgta gaaaatcaag cgagcgatcg
     1981 tgcatatcgt attggacaaa ccaaagaagt ctttgttcat aaacttgttt caaatggaac
     2041 tattgaagaa aaaattgata aattaattga gtctaaacaa gaactcgcaa attccattct
     2101 tgcctctagc caagaaaaat ggattactga actagataca aacgaactaa tggacttatt
     2161 aagattggat gtgtagatta tggcaaaata ttggacagca agcatgcaat attcgcaaat
     2221 gagcgtagaa gaattacaaa aaaaagcaca agaaagtgtt aaaaacgcaa aaaacaaagg
     2281 aaaagaaatg catcccatta ttgtggacgg aaggcaaatt gcaaaaagct ggtggggcat
     2341 ggcctggtgt aaaaatatcg aacaatatgc cgattatgcc agtcgcatcg aacgaggaaa
     2401 aagatatgtt cgttccggtg ctgttgtgga cttacaaatt gttaagggta aagtaaaagc
     2461 acgtgtacaa ggaacaagaa aaacgccata caaagtagaa atcaatatca gtccactaaa
     2521 agaagaaatt tgtgaagaca tcattgaaaa atgtagttca caaatccaaa acttacaaga
     2581 acttgtgaac ggacaattcc cagaaagttt aaaagaaata ttcctatctg aaaacggact
     2641 cttcccaagc caaagagaaa tcagtttcaa ctgtagctgc cccgactggg ccttaatgtg
     2701 taagcacgtt gctgccgtac tctacggaat tggaactaaa cttgacgaaa acccattctt
     2761 attctttgaa ctcagaggaa tcgatgtcaa taaacttgta gatgtaacca tcaaggacca
     2821 cgtagactcc ctactagaac atgccaatat gccaccaaca gatcgtgtaa tggatgattc
     2881 ttgtatcgaa acattgtttg gattaaagta atacaataaa gacactcatt tacacgaacg
     2941 agtgtctttt acatttttct atgataatcc atgtaattct tagtatttct actagtgcca
     3001 ttaaaatcaa ggcaacatca atatatatac atcatcttct cctacaaaaa aattgaagga
     3061 tctgtaaaga acacaaacag atatcatttt taattcccgt aaattgttgc gattgttcct
     3121 acttcatcta aaacgactaa ttttcattga attagatagg attcaacttg acttttatat
     3181 atatcaattg tttaataatc atacagaaaa gctactggat acagtggttc ctctgaattt
     3241 aactaatgta aagacactag taatccgttg ggtttgggtg aactagtgtc ttttacattt
     3301 ttctataatc aataatgtaa ttctaagtat ttctactagt gccattaaac tcatggcaac
     3361 atcaacatat agataaatat acataaaaca cttcctccta cataatcgga atcttcgaga
     3421 actcaagata tcatcctttt aattcagagg gttcaccgtt actacatcca gtagccaatt
     3481 tccattataa aggtccaatg tgaaaacatt atgaattcca atacgattgt tcctatttca
     3541 tctaaaacga cttattttca ttgatttaga tagagttagc cttgacattt aaatatatcg
     3601 attatttaat atacatacag aaagactact ggatatagtg gtcactctga attattttat
     3661 gtcaaaagac actagtaatc cattgcgctt acgggaacta gtgtctttta catttttcta
     3721 tgataatcaa tgtgattctt agtatttcta ctagtgccaa taataccatg gcaacatcaa
     3781 tatatagata tatatgcata gacatctcct cctcttacaa aaaaaattga aggatctgcc
     3841 gagaactcaa gcagatatca tttttaattc agagggttca ccgttactac atccagtagc
     3901 caatttctat tataaagatc caatgtgaaa atattgtgaa ttccaatacg tttgtaacga
     3961 tagttcctat ttcatcaaaa atgccttatt ttcattgaat tagatactct aaatccataa
     4021 atttatatag aaagagaacg tcgagtaagc gttctcttga agaaattcta aaataatagt
     4081 tgcaatccca tcacaatgac ccacatataa gcacaagtct taggaatcat caacaatcca
     4141 atcattggct ttttcttaga ccacaatgta actgggatgt agcaaccaaa cgaaataata
     4201 atagctgcaa ccatacggtt taagtgtaaa tcatacatat tccctgaaat cgcataaaga
     4261 agaattacac caattccagt tacggcaaat acagcattac gaattaaaga caacttccta
     4321 tttccttctt cactcgtcca attgttttgt ggcaagaaac atactacgat acgaataata
     4381 gctaaaatcc aaatcatagc ttcaatccaa agtggagctg taagcgttgg gaaagttaat
     4441 ttccagataa acaataagat aatatagaat actgtcattg tgattgaaga tacctgcaat
     4501 ccaataccaa gttgtctttt gattttgtca tttgttccat aaaccgctct tttaatacga
     4561 ggaaccaagt ggaatgcatc tccaccacac aatgttaatg ttaatacacc atataaaata
     4621 aataatggat ttccttttga atatacaaag aataagattc cggcaattaa gtcaaagatc
     4681 aagtaacaag catcaaaaat agcttccatt aaatccggca ttttaggttt ataattattt
     4741 ttactcattt gaaatcatac ttgctgcatt gtcatacacc ttttgaaaaa cattcataaa
     4801 caccttgatt tcttcttccg ttaggccatc aaacatgtct tgcgcaaact ctttttgcgc
     4861 ccacttacca tcttcaatca cttcattagc caattgaagc aaatgaattg tgacacgctt
     4921 tttgtttgca ggatccactc ttctttcaat caagccacga tcttctaata ctttaagtga
     4981 agttgaaaca tgagactttg tagacttacg aatacgaaca atatccgccg ccgtattgta
     5041 ttttggatta ttgtaaagaa acatcaatat gtcatattcc atctgacgta agtcatactt
     5101 ttcacaaacc cccgccatct tcatctcata atatctcgta atcgttttat gttcatccca
     5161 aaagtgcata tgcattctcc ttatcgttct atttagaaca ttaatattct atagctcatg
     5221 aataggtttg tcaacaaaat cattgacacc attcttaaaa aaagggatac tctgattagg
     5281 gaagaagggg ttaagaatgc gtacacaaaa atataaccta caaggaataa aagtattctt
     5341 ctatatctta atgattgcca ttttaggatg gttttgtttc atgcaactct ttggagccaa
     5401 cgaacaagag catgatattc aatctcattc cattgtctat acacaaacgt tttattggga
     5461 aaaagaagat ggttccaaac aaaaaataaa tgtcccagga aaatatgaag taaaaccagg
     5521 ccatacaatg gttattcgta caactcttcc taaagattat aatgaaactt cctttgcgat
     5581 tcgctcctcc ttacaagatg ttaaattcta tgtagataat acattacgta aagaatattc
     5641 tacaaaagat acgcgccttg ccggtaaaaa ttcggcgagt cgatatatat tttgtcccac
     5701 aacctataag gatgcaggaa aaacattacg cattgaactt acaacctata cttctaacta
     5761 ttctggtgta gtcaacgaaa tttattgtgg aagtgaaatc gatatctggc aacatatata
     5821 tagcgattat aaaatgtcca cattcattgc cttcttcatt ttatttgccg gtattacatc
     5881 cgttctattt ggttcagccc taaaatttac ttatcacacc aatttcagaa cggaatattt
     5941 aggctggtgt atgatcatgg gagccatctg gatgcttgga gaatccaaaa tccgccaaat
     6001 cttagtacca aacgcatctt ctctagcctc attatgcttt gtgatgatca tgcttagtcc
     6061 tttaccaata ctcttctatg ccgatagcat ccaaaatgga aagcatcgaa agctctataa
     6121 atatattggg tgcattgccc ttttaaactt tgcggtttca tccatcctat atttagctaa
     6181 agtcaaagac tacattgaaa cattgcctgt tgcacaatgc attctgatct gtgtatttat
     6241 cttggtattt attcatttat gccaatacat acgcaaatac agtcattccc gctcagacta
     6301 cttcttactt ttcggactct ttttagtctt agtctgcata gccattgaaa gtatatccgt
     6361 ctattttgtt gccatgattt ccggcatctt tatcggtatc ggcatgattg tattactact
     6421 cacaaacatt gttcgtacca tccatagaat tcaacttatt gaacaaacac gtcatcaaaa
     6481 ggaattggat attgaaaaga aagaaaacaa aaaaataaca cttcaaatga tgcaatctct
     6541 ttcgacaacg attgaggcaa aagacgaata tacacgtggt cattcaaggc gtgttgccca
     6601 atatgctgct ttgattgcga aaaatcttgg ttggacagat caggaaatcg aaaacattaa
     6661 aagctgtgct tatttacatg acattggaaa gatcggtatc cctgatcaaa ttttaaataa
     6721 accaggtcaa ttaacggaag atgaatttaa tttaatcaaa caacatccga ttattggaca
     6781 agacatccta aaagatatca caatcatacc tcacttaggt gaagtgacaa gaagtcatca
     6841 tgaacactat gatggtactg gctatccaga tggactaaaa ggtaatgaaa ttccaattca
     6901 agcacgtatc attacgcttg ccgatagtta tgatgccatg aattcaaaaa gaatctatcg
     6961 taatgcgctt agttttaaac aaatcaaaga agaaatcgaa aaaatgcagg tacacaattt
     7021 gatcctgaaa tcaccaaaat ctttcttaaa ctcatggacg atgaatcttt atttaactta
     7081 gaacacgaaa ttattccggt tcaagaaaat acaatcgata aatttatatc cgacgttgta
     7141 tcgactatta aaaatcagga agaaacaaag aaccacgact atcttacagg tcttcctgta
     7201 cgtagtgtag gccaaaagta tatagcgatt gcgatgcaaa aaacaacggg atgccttatc
     7261 tttttagata tggataatct taaaaaaatc aatgatgtct atggacataa agcgggagat
     7321 agagctttaa aaagtttagg aaaactatta ttagacattc cattagataa aatcacatgc
     7381 agaatgggcg gagatgaatt tctactattc ttaccaaaag taaattcaga acaagccgaa
     7441 aagattctgg ctcaagtaat cgaacaattc aaaggtatcg tagaaaacga tgatgaaatt
     7501 cattttgcga cactctctgc aggtatgctc atgactacaa aaaatgataa ttttgaagat
     7561 gcttgttcaa aagctgacaa agccttatat tatgtgaaac aaaatggtaa aaacaactat
     7621 tctttctaca atcaaattca acataaaaac acatccaata atacggcaga cctaaaacaa
     7681 atcgccaaat ccctacaaac aagtggaagc tatacaggcg ctttaagtct agactttaga
     7741 gaattcactc gacactatga gtacatacat caattaatgg ttcgaaatca ttcttgttgt
     7801 tatttggtga tggtcactat ggaaactgta gaagacacac ttccatatat tgaaaaaatt
     7861 gaagaagcat taacacatat gggtgaagcc atccacaaaa acattcgaaa agtcgatgtt
     7921 tgtacacgat atagtgccat gcaatatttg atcattctat cccaaccaac agaagcagaa
     7981 atcccaaata ttatgacacg catttttata cagtactata agcttcaaga cagccaaaac
     8041 ttcacaccaa cctatgaata tatcacaatg aaggattaaa ggagatcgac tcacatgtca
     8101 atctcctttt ctaatctaac aaccacttcc atgcaggaac cacatctatc tttattccag
     8161 cacaatttaa ttttgtttcc tcactatttg taacaagaag acactttgta tctgggataa
     8221 attgattcaa tttcacaaac gccttcgttt cacgttctaa ggtatctaca tcctctaaca
     8281 cttgcatact cacctgtatt gccaaatttt cacttggaat ataaaaatct acttcgatat
     8341 tattttcaaa gaaatacaca ttttcaaatc catatcttcg aatcagctca accgcaacca
     8401 aattttccaa ttgagctgtt ttacaatcca acaacatcaa tcccaacagt ccagtatcca
     8461 tgaaataata ttttggtgat gttttttttg atgcatagtt ctgtagtgaa aatattaaat
     8521 atgaatctaa catatgtcct acataattga ttaccgtttg ttttccaatc gaaattccag
     8581 cacttttcac tatattcgtt aaccgattaa aagacaaagc tttcgttaca gactctgcaa
     8641 tcttctttaa aattaatcga accgcaaaat catttgtaat tttgtttctt gtaataatat
     8701 cctgtaaata aacagtttga taaatattat ttaaaaatgg tcttttattt ttaatatcca
     8761 ccaattcagg aaaagctcca taagtcacat actgttcata ttgacttaca atatctgcac
     8821 gcttttttgt acctatttga tcaagccgta cgttctcaat atgatttgca ctcaaatatt
     8881 ccttaaatga atatggaaat acattcacaa tcatgaatct tccacctaaa gtagaagcta
     8941 tttctttgct taacatttta ctattgcttc ctgtgatatt gattcgatac ttcatgtccg
     9001 caactctacg aacaaatttt tcccatccat caacattctg aatctcatct aaaaataagt
     9061 atggtttccc tttcgaacca gaaaattcaa taccaagctc taatattgta tttaaatcat
     9121 ccacaccaat ctccaataga cgctcatcct cgaagttcac atatacaatt tgtgacgatg
     9181 aaattccatc attcattaag ttgtgaatct gttgatacat catatatgat tttcccattc
     9241 ttcgaatacc aacaaaacaa taatttacat tttcttctaa atcataatcc ctagaaatca
     9301 atggattatc aagatacatc tccttttgat ctattattat ttgtttcaaa acatctcgat
     9361 tcatatacat caaccttctt tatcttatca tatgcagata ttgtcttacg tacaagacaa
     9421 tattaacact tttttgtctt atgaacaaga cagcattcat atatttttgt cttaattaca
     9481 aaaaaagaca ccgattactc gatgtctata attcttctcc atttgtttca atcacatgtt
     9541 tgtaccagta aaagctatct tttttcatac gtttaccagt acctttgcca aaatcatcat
     9601 aatctacata cacatatcca tagcgtttac tcatttcagc acttgatgaa gatacaatat
     9661 caataggccc ccaacttaaa tacgaaaata cattcacacc atctaaaaca gcttctttca
     9721 tctgttcaat atgagctttt aaatattcaa tacgatactc atcatgaata gagccatctt
     9781 cttccactac atcttctgca cccaaaccat tttctgcaat catcaaaggc acttgataac
     9841 gatcccacaa ttgatttaga caattataga atccaagtgg gtctgcacgc cattcccatg
     9901 gggttggttt taaataagga ttttgttcag atttaaatgg tactcctgta tctgtttcgt
     9961 gattaaatac atttgtatag taatacgata aagctaggaa atccatcgta ttatttttca
    10021 ataattcttc atcaccatct tcaaaatgaa tatgaatacc tttttcttcg aaatgtctta
    10081 acatataagt tggatattca ccacgcaatt gtgtatctgt aaagaaatat tcttgtacac
    10141 gattacgttt catggctccc acaatatcct ttggattaca tgttttagga tatacgcacc
    10201 cttctgcaat cattgttcca atatgtgcct ttggatctat ttcttttgca aattctttga
    10261 tttttgcaca tccaataaat tgatgatgca cagcctgata cattgtttct tccatttttt
    10321 ctggagcaac ctgatcatca taaattccta aacaaccaaa ttgtacattt ggaatcaaat
    10381 tcacctgatt acaaacaatc caatatttaa cttttccctt ataacgctct agtaatactt
    10441 ttccatatcg aacaaagaaa tcaatcactt tacgatttgc gaagccacca tattccgtta
    10501 ctaaatgaag tggaatatca taatgtgaaa tcgtaatcac tggttccata ccatctttaa
    10561 tgatttcatc aatcaaatga tcatagaatt caagaccctt ttcatttggt tccaattcat
    10621 ctccatttgg aaaaattctt gaccaagaaa tacttgtacg aaatgcttta aatcccattt
    10681 cttttaaata agccaaatcc tctttataag tatggtaaaa atcaatacca taacgtttag
    10741 gataatatcc ctctttatca gcaatcgcag ccttaatacc cgctaatgtg ccaccacctt
    10801 ctttttcaat atgagcacga tcctggtttt tatcatattc atgaatgtct gatgtagaaa
    10861 gtcctctacc atctacatca tacgcacctt ccatttggca agcagcactc gctcctcccc
    10921 ataaaaagtc tttaggaaat cccgttggca attgtttcat tttattttcc tcccaaatct
    10981 tctccatttg tttctattac acgtttatac caatcaaagc tatctttctt aattcgtttt
    11041 cctgtaccat ttcctaaatc atcataatct acatatacaa agccataacg ctttgtcatt
    11101 tcagatgatg tacaacttac gatatcaata ggtccccatg ggtaatatcc aatcaattcg
    11161 acaccatcat aaatagcttc tttcatttgt tccatatgtt ccttatgata cgctatacgg
    11221 tagtcatcat gaattttgcc ttctgaatct ggtttttcat caaatccaga acctaactca
    11281 gtaatcataa ttggcaattg ataacgatca tagtattcat ttaatacata acgtaatccc
    11341 accggatcat tgatccatcc ccactcattt tcttttacat atgggttttt ctttctttcc
    11401 ccaggtttaa gatcacttga acatacaccc gttccataat aagagaaagt taagaagtca
    11461 catggatact tttcaattaa atctaattct tcttttgttg cttcaaaggg tttaatttta
    11521 ttttcatcca tatatctttg taaagaacga ggatattttc ctctacacaa tacatctaaa
    11581 aacatatatt gaatttgatt cgacttacaa gatgccatca cttcatttgg atcacaattg
    11641 aacggataag atataccatt acaaaccata catcccattt tcattcccgt attcatttcg
    11701 tccattttct tttttgcata tgcactagct acaaattgat aatgcaagcc ttggaaacat
    11761 gcagaagtaa aatcatctgt ccaatcataa ggaatagata aactgttata cccttcgcca
    11821 aataaagtat ttatttgatt aaatgcaatc caatatttaa ctttgttttt atagcgttcc
    11881 ataattacat ctacaaatct tttaaatgat tctaatgttt ttttactata ccatccgcca
    11941 tatttaatag ataaattcaa aggcatctca tagtgagaaa ttgtaaccat aggtatcatt
    12001 ccatcaagaa tcatttcatc aaataaatca tcatagaatt ttaaaccttc ttcatttggt
    12061 tctttttcat ctcctcgagg gaaaatacga gtccatgcaa tagaagttct aaat
//
LOCUS       DBMDWL010000054        11870 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1835_length_11870_cov_2.904835, whole genome shotgun sequence.
ACCESSION   DBMDWL010000054 DBMDWL010000000
VERSION     DBMDWL010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11870)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_11870_cov_2.904835"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            527..1162
                     /locus_tag="ACZPDN_05170"
     CDS             527..1162
                     /locus_tag="ACZPDN_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1298045.1"
                     /translation="MNKSKLSVRQMCVLALFLAIEIIMYLTPIGYLRINALSVTLMHV
                     PVIILASTMGPTAGAFLGFVFGCTSVIGATMTPGITSFVFSPFITVGGISGNFFSLVI
                     ALVPRILLGVIAGYVFRFAKKYAKNTPVAAGIAAAVATVCHTIMVLGLIVILFGPQYS
                     QALGISQAALNGVMAGVIGTNMIPEVIVAVVSNMALATALSSRYVGIAQQA"
     gene            1231..1776
                     /locus_tag="ACZPDN_05175"
     CDS             1231..1776
                     /locus_tag="ACZPDN_05175"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1298046.1"
                     /translation="MDLNWKLNKTEHIVQDEWIDFRKESFILPDGKEFSPFYNYSRRN
                     FVVIIARTKDDKYITVKQFRHGLKKVTTEFCAGGIEGSEHESALLAAKRELEEETGYI
                     SDNWKHIYTIPSNPTIADNYAILYYADNCEKACDQHLDSTEFLEVKEYTEDEINDLIK
                     NNEFVQPIHLMGWLMLKKGVL"
     gene            1800..2084
                     /locus_tag="ACZPDN_05180"
     CDS             1800..2084
                     /locus_tag="ACZPDN_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1298047.1"
                     /translation="MEKLTKKDIISNIAEDSHLTKKDITEVVDLALTQIFEGLSAGKT
                     VDLAGFGKFEITERKAREGINPLTKEKIQIEASKSVKFKIAKALKEAVNK"
     gene            complement(2299..3156)
                     /locus_tag="ACZPDN_05185"
     CDS             complement(2299..3156)
                     /locus_tag="ACZPDN_05185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1298048.1"
                     /translation="MGITPKRLLKELKRNQISNVVADMPVYKGMEIYVVDAKTKKILG
                     ATDASWVGNTAHFNHKGYRFVKRKHGDYIVAVCMLESMNAQTNIVAILIVGIYLTLAS
                     CLLVLMLSRVLKEKYEKEKYIYTSNTDELTKCYNRRAYDSDMENLDLNTEWIYISLDL
                     NGLKKANDTLGHSAGDELICAASNCMKFAFASYGKIYRIGGDEFVVLIQESVSNIDSI
                     LQAFDTTIHDWHGKYSNSISVSYGVVKSSEQDFDSIHSVSKLADERMYKSKSEFYNIS
                     GNDRRSYNG"
     gene            complement(3157..3675)
                     /locus_tag="ACZPDN_05190"
     CDS             complement(3157..3675)
                     /locus_tag="ACZPDN_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298049.1"
                     /translation="MRKRKILILSITIVAVALILETCILQYVNDKNAQTTSNVLLDRV
                     IAVIDKNESIESEFVKSLKDDYIVRAKAISYIVDAKPEVEYDVDELQKIAGLMSVDEI
                     HFIDDTGTIYSGSLPKYFGYSFDSGEQMAFFKQMLNNKDLTLCQDVTPNTAEAKEMMY
                     AITWNEAKTHMV"
     gene            complement(3722..4351)
                     /gene="thiE"
                     /locus_tag="ACZPDN_05195"
     CDS             complement(3722..4351)
                     /gene="thiE"
                     /locus_tag="ACZPDN_05195"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1298050.1"
                     /translation="MKKVDYTLYFITNSDGYDEETFLRKVKGACLGGATIIQLREKNK
                     STLEYYELALKVKNITDAYNIPLIIDDRIDVAMAVGCGVHLGNEDMPISQARKIMGED
                     CIIGATAKTVEVAKNAEADGADYLGCGAIYPTKTHVKTKITSVETLNDICSSVNIPVC
                     AIGGLNKDNLSVLEKSPIQGVCVVSAIMDADDTKLAAEELKKSIQKIVA"
     gene            complement(4348..5118)
                     /gene="thiM"
                     /locus_tag="ACZPDN_05200"
     CDS             complement(4348..5118)
                     /gene="thiM"
                     /locus_tag="ACZPDN_05200"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR1298051.1"
                     /translation="MLKQTNNLIHCITNPISINDCANLILALGAKPIMACHPDEVESI
                     TSNSSALALNLGNFDDIRAKSMMISAKCAKENKIPFILDLVGVACSTLRLNYAKELVS
                     VACPTVIKGNISECKAFYGMTSHAHGVDADIQDEEDIYESCKWLKEMALNLGCVVVCS
                     GKIDVITDGKEVNLISNGCDMLSRITGTGCMLNVAIATFLASYSPLLACIKATCYYEV
                     AAEKTECSGPGTFHMHFMDEIYKLSSFDESLFKIEVLG"
     gene            complement(5112..5633)
                     /gene="thiW"
                     /locus_tag="ACZPDN_05205"
     CDS             complement(5112..5633)
                     /gene="thiW"
                     /locus_tag="ACZPDN_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HIR1298052.1"
                     /translation="MNKHNYLLSLVFFSMMVAVGVVISPILRVEGMCPMAHLINITMA
                     VFMGPWYSFLCALAIGLIRMTVMGIPPLALTGAIFGATLSGILYRVSKGKIWAAVLGE
                     VIGTGLIGSVLSYPVMTYLWGRTGLTWMFYVPSFLMGTIIGGSIAYVLLKSMSRSGIL
                     ENIQRKLGVQNIC"
     regulatory      complement(5820..5916)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6044..6796)
                     /locus_tag="ACZPDN_05210"
     CDS             complement(6044..6796)
                     /locus_tag="ACZPDN_05210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1298053.1"
                     /translation="MDYVYVTKENIEQEHICCAISNDSDVQVRSKKDWLSDRFDDGLV
                     FIKSSVRGKCFIEYIPAKNAWIPLDAKNFMYIDCLWVSGSLKGHGYSSELLNMCIEDS
                     KKKGMDGICILSTKKKTSYLADPKFLAYKGFKVCDEANNGIQLWYLSFKDSVSPKFKS
                     CAKDCHIDELGFVLYYTNQCPFNAKYVPVIENVSKDLNIAFKAIHLDTKEKAQNAPTP
                     ITTYALFYNDEYMTNDVMNEKKFIKLLSNLGL"
     gene            complement(6860..8155)
                     /locus_tag="ACZPDN_05215"
     CDS             complement(6860..8155)
                     /locus_tag="ACZPDN_05215"
                     /inference="COORDINATES: protein motif:HMM:NF025019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1298054.1"
                     /translation="MYRKIMNFLEEWKESNHRKPLILQGARQVGKTYSILEFGRLHYE
                     NVAYFNFETNPKLNETFEENISPDYLIPILSHIAGQTIVKEKTLIVFDEVQLCERALT
                     SLKYFCEDAPDYHIIVAGSLLGVAVNRSKFSFPVGKVDMKTLYPMDMEEFMFALGEDD
                     LVVQIKKSFQTDTPLPSALHDAAMQLYRQYLVVGGMPECVMLFAQTKDYILVRHTQDT
                     ILASYLNDMSKYNNLNEIKKTRLAYDNITVQLSKKNTRFQYKLIKKGGRASEFENAIE
                     WLCLSGIVSQVYRVEQIKKPLENYRDIDAFKIYVSDLGLLCAKKDLAANDILYMVEEL
                     NDFKGGMTENYVNAQLIINGYHTYYWQSDRGAEIDFIIQRDGKLIPIEVKAADNARAK
                     SLKMYMDTYKPAYAIKLSSKNFGFEDNKKIVPLYAAFCI"
     gene            complement(8314..10944)
                     /gene="ppdK"
                     /locus_tag="ACZPDN_05220"
     CDS             complement(8314..10944)
                     /gene="ppdK"
                     /locus_tag="ACZPDN_05220"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR1298055.1"
                     /translation="MDKKYVYEFNEGDETMRELLGGKGANLAGMSKLGMPVPYGFTIT
                     TEACNQYYEDNETINDGIKAQIMEYLDLLEKKSGKKLGDEANPLLVSVRSGARASMPG
                     MMDTILNLGMNKTVAETVANLTNNERFAYDSYRRFIQMYSDVVMGLSKKRFEEIIDEV
                     KAERGITDDLDLNAEDMKKLVELFKAFYKNELKSEFPEEPKEQLMGAIEAVFRSWNNP
                     RAIYYRKMNDIPSSWGTAVNVQMMVFGNMGNDCGTGVAFTRNPSTGENKLYGEFLMNA
                     QGEDVVAGIRTPQKIDQLKEVAPGAYDDFVAITKKLETHYRNMQDMEFTIEKGKLFML
                     QTRNGKRTAQAALKIACDMVDEGMITTDEALMMVEPKQLDSLLHPMFDADELKKAEPI
                     ASALPASPGAACGQIVFSAEEAIQEASRNHKVILVRLETSPEDIEGMHVSQGILTVRG
                     GMTSHAAVVARGMGTCCVSGCGDINMHDDEGYFEIDGVKYHRGDWISLDGSTGNIYGS
                     AIKTVPASISGDFERFMNWADERRTLKVRTNADTPHDAKQAHEFGAQGIGLVRTEHMF
                     FEGDRIKAVREMIVSKTAAQRRVALEKILPMQRSDFEGIYEAMQGLPVTIRYLDPPLH
                     EFLPTNSYDITQLAKDMHIGLDELKSVISSLHEFNPMMGHRGCRLAISYPEIAEMQTT
                     AVIQAALAVNERHPDWKIEPEIMIPLVGDVAELRYVKKVVCDVADKLIQESELDMKYH
                     VGTMIEIPRAALLADEIAKEAEFFSFGTNDLTQMTFGFSRDDAGGFLQDYYDKKIFEQ
                     DPFAHLDQRGVGKLVKMATELGRSTRPNIKLGICGEHGGDPASIEFCHRVGLNYVSCS
                     PYRVPIARLAAAQAAIKFDK"
     gene            complement(11066..>11870)
                     /locus_tag="ACZPDN_05225"
     CDS             complement(11066..>11870)
                     /locus_tag="ACZPDN_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HIR1298056.1"
                     /translation="TEIFNPWSVINYISDQCFPKAFWQSTGSNDIIGEIIGTATPEIT
                     ENLYKLFCGNTITTYVDTSVIYPEVQNNPYSIYSFLLVAGYLKVAAIYPQNDGNYMCD
                     VAIPNKEILYVYEKEVLNRTNQNNVSISIHQAIFSKDTRKLQSLLEDFMLKSISTMDG
                     ANEAFYHRMMLGLCAVLGSQYKVRSNRESGLGRFDVELLPMMQGIPGFIFEFKHTKDI
                     NVDLDSLANSALRQIDDMKYDTELKDFGVKNIVKIGIAFRQKSAVVKRG"
BASE COUNT         3907 a         2390 c         1681 g         3892 t
ORIGIN      
        1 tttcataata atagtgtaca ccaatacact attaaagtat ataaaaaaat aaataatctt
       61 caaacatgaa aaattgaaga ttatttcatg cgtaatcact atctgtaatt tgcttaattg
      121 tatttttgga gaaactgtgg tatattgaag gtgacgaagg agtgcactgt ggcgctcagc
      181 ctggagggat acgtccttcc agttttttat tcatcagaaa gaatcgaaat tattaatttt
      241 atgattttaa tttttttagt gttgtaatca ttttcatcta ttgatggtat attaaatatt
      301 ttatcatgta tcaaacatat tatgatatag aacaatgatt ataattcctc ttctccattc
      361 aacattggga actaaaacag ggacttgatt cctgtttttt tttattgtaa actattgaag
      421 atgttttttt tgtggatatg gatcgcatga ccataatcta tgtatgaaga catctatttg
      481 attagttaca tcgtattctt aaaaagaaac gaaagtagga ggaaatatga acaaatcaaa
      541 actatcagta cgccagatgt gcgttttggc tttattttta gccatcgaaa tcatcatgta
      601 tctaacacca attggatact tacgtatcaa cgcattaagt gttacattga tgcacgtgcc
      661 tgtaattatt ttggctagta caatgggacc tacagccggt gctttcttag gattcgtatt
      721 tggttgtact tctgtaattg gagctacaat gacacctgga atcacaagct ttgtcttctc
      781 tccatttatc acagttggtg gaatttctgg taacttcttt agtttagtta tagctttagt
      841 accacgtatt ttgttgggtg ttatcgcagg ctatgtattc cgttttgcga aaaaatacgc
      901 aaagaatacg ccagtcgctg caggtattgc agccgctgtt gcgacagtat gccacacaat
      961 catggtatta ggattgatcg ttatcttatt cggtcctcaa tactcacaag ctttaggtat
     1021 tagccaagct gcattgaacg gtgttatggc tggtgtcatc ggaacaaata tgattcctga
     1081 agtcatcgta gctgttgttt caaatatggc tttagcgact gcattaagtt caagatacgt
     1141 tggtattgca caacaagcat agtcaatgaa tagtgtaact ttggttgcac tatttttttt
     1201 attgggatta taataatttg ggtgataaaa atggatttaa attggaaatt aaataaaaca
     1261 gaacatattg tacaagatga atggatcgac tttcgtaaag aatcttttat tcttccagat
     1321 ggaaaggaat tctctccttt ctataactat tcaagaagaa acttcgttgt gattatcgca
     1381 agaacaaaag atgataagta tataactgta aaacaattca gacacggcct gaaaaaagta
     1441 accactgaat tttgtgccgg tggcattgaa ggaagcgaac atgaaagtgc gttacttgct
     1501 gctaaacgtg aacttgaaga agaaacgggc tatatttcag ataactggaa acatatatat
     1561 acaattccta gcaatccgac aatagcggat aattatgcaa ttttatatta tgctgacaat
     1621 tgtgaaaagg catgtgatca acatctagat tcgacagaat ttttagaagt caaagaatat
     1681 acagaagatg aaatcaatga tttaattaaa aacaatgaat ttgtccaacc aattcatcta
     1741 atgggttggc taatgttgaa aaaaggcgta ttatagggat gtaaaggaga cttataaaca
     1801 tggaaaaatt aactaaaaag gacatcattt ctaacattgc cgaagattct cacttaacta
     1861 aaaaggacat cactgaagtt gttgacttgg cacttacaca aatctttgaa ggtttaagtg
     1921 caggaaaaac agtcgatctt gcaggatttg gaaaatttga aatcacagaa agaaaagcta
     1981 gagaaggaat taatcctcta acgaaagaaa agattcaaat tgaagctagc aaatctgtta
     2041 agttcaaaat tgccaaagca ttaaaagaag cagttaataa ataatattaa aacaggggct
     2101 ttaacgaata agtaaatttt attacttatg gagttcagct ccttttcttt ttgtttgatt
     2161 agctccaata taaaaaatga gcatcactat ttgtgatact cctttaacaa gaactgtcag
     2221 atttttcctt gacttttaaa ccagtttaaa tttttaaatc ttgaatattc ggtctttttt
     2281 tattaagttg aaacttcttt agccgttata gcttcttcga tcattaccac ttatattata
     2341 gaactcagat ttactcttat acattctttc atccgctaat ttagaaacag aatgaattga
     2401 atcaaaatct tgttcagaag acttgacgac accatatgat acagatattg aattggaata
     2461 cttaccatgc caatcatgaa tagtcgtatc aaacgcttgt aatatagaat ctatattcga
     2521 tacggattct tgaatcagta cgacaaattc atctccacca atacgatata tttttccata
     2581 agaggcaaac gcaaacttca tgcagttact agccgcacaa ataagttcat cccctgcact
     2641 atgtccaagt gtatcattgg ctttttttaa accatttaaa tccaaagata tataaatcca
     2701 ttccgtattt aaatctaaat tttccatatc cgaatcatag gcacgtcgat tgtaacattt
     2761 cgtcaattca tccgtatttg aagtatagat atacttttct ttttcatact tctcctttaa
     2821 tactctagaa agcattaaaa ctagtaagca tgaagctaaa gttaaataaa tacccactat
     2881 caagatcgca acaatattag tttgagcatt cattgactcc aacatacata cagctacaat
     2941 ataatctcca tgtttacgtt ttacaaatcg atagccttta tgattgaaat gagccgtatt
     3001 tccaacccac gatgcatctg ttgctcctaa aatctttttt gtctttgcgt ccaccacata
     3061 gatttccata cccttataca caggcatgtc ggctacgaca tttgaaattt gatttcgctt
     3121 caactccttt aaaagacgct ttggcgtaat acccacttaa accatatgcg ttttcgcttc
     3181 attccaagta atcgcataca tcatctcttt agcttcagcc gtatttggcg tgacatcctg
     3241 gcataatgtt aaatctttat tatttaacat ttgtttaaag aaagccattt gttcacctga
     3301 atcaaaacta tatccaaaat atttaggtaa cgaacccgaa tagatcgttc ccgtatcatc
     3361 aataaaatga atttcatcca cagacattaa gcctgcaatc ttttgaagtt catcgacatc
     3421 atattccact tccggcttag catctacaat ataagatatg gccttagcac gaacaatata
     3481 atcatctttt aaagatttca caaattcact ttcaatcgac tcattcttat ctataaccgc
     3541 aatcacacga tctaataata cattagatgt tgtttgtgca ttcttatcat tcacatactg
     3601 caatatgcaa gtttctagaa tcagggccac cgcaacaatg gtgattgaaa gtattaaaat
     3661 ttttcttttc ctcatattta ctattttagg ttatatttta caaaaataaa agtggcattt
     3721 gttatgccac aatcttttga atcgattttt taagctcttc agccgccaac tttgtatcat
     3781 ccgcatccat aatagccgat acaacacaaa caccttgaat aggactcttt tctaaaacag
     3841 aaagattatc tttatttaat cctccaatcg cacaaaccgg aatattgaca gacgaacaaa
     3901 tgtcatttag tgtttcaaca cttgtaatct ttgtctttac atgcgtcttt gtcggataaa
     3961 tggcaccaca acccaagtaa tcagcaccat cagcctctgc atttttggca acctctactg
     4021 tttttgcggt agctccaata atacaatctt cacccataat tttacgagct tgtgaaattg
     4081 gcatatcttc atttcctaaa tgaacaccac atcctacagc cattgcgaca tcgatacgat
     4141 catcaataat taatgggatg ttataggcat ccgttatatt ttttaccttt aatgccaatt
     4201 cataatattc caatgttgat ttattctttt cacgaagctg gataatggtt gctccaccca
     4261 agcaagctcc ttttacttta cgcaagaatg tttcttcatc ataaccatct gaattggtaa
     4321 taaaatataa tgtataatct acttttttca tcctaacacc tcaattttaa acaaagattc
     4381 atcaaaactt gaaagcttat agatttcatc cataaaatgc atatggaaag ttcctggacc
     4441 agaacattct gttttttcag cagctacctc ataataacaa gttgccttaa tacaagccaa
     4501 taatggagaa taactagcta aaaaagtcgc aatcgcaaca tttaacatac atcccgttcc
     4561 tgtaattctt gaaagcatgt cacaaccatt tgaaatcaaa ttaacttctt taccatccgt
     4621 aatgacatca attttaccgc tacaaacaac tacacaacca agatttaaag ccatctcttt
     4681 tagccactta caactttcat agatatcctc ttcatcttga atatccgcat ctacaccatg
     4741 cgcatgactt gtcattccat aaaaagcttt acattcacta atattcccct taataacggt
     4801 tggacaagct acagaaacca gttctttagc ataattcaat cgaagtgttg aacaagccac
     4861 tccaaccaaa tctaaaatga atggaatttt attttctttg gcacatttag cactaatcat
     4921 catagactta gctcttatat catcaaagtt acctaaattt aatgccaaag cacttgaatt
     4981 tgaagtaatc gattcaactt catccggatg acaagccata atcggcttcg cccccaaagc
     5041 taatataaga tttgcacaat cattgattga aatcggattt gtgatacagt gaatcaaatt
     5101 attcgtttgt ttcaacaaat attttgcact cctaactttc tttgaatatt ttctaggatt
     5161 ccactacgag acattgactt caataaaaca tacgcaatac ttccaccaat aattgtaccc
     5221 attaaaaagc taggtacata gaacatccaa gtaagtcccg ttctacccca taaataagtc
     5281 attactggat atgaaagaac agaaccaatt aaacctgtac caatgacttc accaagtacg
     5341 gcagcccaga tttttccttt acttacacga tataagattc cagataatgt agctccaaaa
     5401 atagcgccag ttaaagccaa tggtggaatt cccataactg tcatacgaat taaaccaata
     5461 gctaaagcac aaagaaatga ataccaaggc cccataaaaa cagccattgt gatattaata
     5521 agatgtgcca taggacacat tccttccaca cgtaaaatag gtgaaatgac cactccaaca
     5581 gctaccatca ttgaaaaaaa gactaagctt aataagtaat tgtgtttatt cataatacta
     5641 cctcctacag atacagatgg cctagaaaac acgcaaaaaa ccatctgtgg tttcggtatt
     5701 agacaaacag tcctctctca tcctgtcaat tcaggctccc tcgcttgttt tctatactct
     5761 aggcgctccc ctacagtaag gcaataaaat aaaaagacct gcaacgtaaa aagacaggtc
     5821 aaaaaaacat ctatacttcc tacgccggca ttatccgtca ggtttaatgg gtcgaagtcg
     5881 tacttcctct cagcaatagc tccccagatt tatttactac atctaaaatt ctagtacaaa
     5941 acacactaaa gtgcaaggag taattcattc ataaaaacat acaaaatgaa attttttatc
     6001 tattattcaa aaccataatg tcaaatgata aagggttgta atgttacaac cccaaatttg
     6061 ataaaagctt tataaatttc ttctcattca tcacatcatt tgtcatatac tcatcattat
     6121 aaaataaggc ataggtagta atgggtgttg gcgcattttg cgccttttct tttgtatcta
     6181 aatgaatcgc tttaaatgcg atatttaaat cttttgaaac attctcaatc actggaacat
     6241 atttcgcatt aaaaggacac tgatttgtat aatataagac aaaacctagt tcgtctatat
     6301 gacaatcttt cgcacaagat ttaaatttag gtgaaaccga atccttaaaa cttaaatacc
     6361 ataattgaat accattattc gcttcatcac acactttaaa acctttatac gcaagaaatt
     6421 taggatcggc aagatacgat gttttctttt tggtagatag aatacaaatt ccatccatac
     6481 ccttcttctt actatcctca atacacatat tcaataattc actcgaatag ccatgaccct
     6541 ttaatgatcc agaaacccat agacaatcaa tatacatgaa attcttggca tcgagtggaa
     6601 tccacgcatt ctttgccgga atatattcaa taaaacattt ccctcttaca ctcgatttaa
     6661 taaagactag cccatcatca aaacgatcac ttaaccagtc tttctttgat cttacttgaa
     6721 catctgaatc attactaatt gcacaacaaa tgtgttcttg ttctatattt tcttttgtga
     6781 catatacata atccatttta aacacctcaa aatcagtata ctaaaaggag ttgaaaccta
     6841 tcaactccta aactaaaaac tagatgcaaa atgcagcata aagtggaact atttttttat
     6901 tgtcctcaaa gccaaagttt ttactagaaa gcttaattgc ataagcaggt ttatacgtat
     6961 ccatatacat ttttaaactc ttagcccgag cattatctgc agccttgacc tcaataggaa
     7021 taagttttcc atcacgttgt ataataaaat ctatctcagc acctcgatct gattgccaat
     7081 aatacgtatg atacccgtta attataagtt gcgcattgac atagttttct gtcataccac
     7141 ctttaaagtc attcaattct tcaaccatat aaagaatatc attggccgct agatcctttt
     7201 ttgcgcaaag caaacctaag tcagatacat aaattttgaa agcatcaata tcacggtaat
     7261 tttcaagtgg ctttttgatt tgttcgaccc tgtagacctg tgacacaata ccagaaagac
     7321 aaagccattc aatcgcattt tcaaattcag aagcccttcc acccttttta atcaacttat
     7381 attggaaacg agtatttttc tttgaaagct gaactgtaat attatcatag gcaagtctag
     7441 ttttcttaat ttcatttaaa ttgttatact tactcatatc attaaggtag cttgcaagta
     7501 ttgtatcctg tgtatgtcgc actaggatat aatccttcgt ttgcgcaaac agcatcacac
     7561 actctggcat accaccaact acaagatact gacgataaag ctgcattgca gcatcatgta
     7621 aggcagatgg taatggtgta tctgtttgaa aagatttttt aatttgtact actaaatcat
     7681 cctcaccaag tgcgaacata aactcttcca tatccatagg atagagtgtt ttcatatcca
     7741 cctttcctac aggaaatgaa aatttagacc tgttaaccgc tacaccaagt aaactacctg
     7801 caacaataat atgataatct ggggcatcct cacaaaaata tttaagcgaa gtcaatgctc
     7861 tttcacaaag ttgtacttca tcaaatacaa ttaacgtttt ttcttttaca atcgtttgac
     7921 cggcgatatg agataaaata ggtatcaaat aatcaggact aatattttct tcaaaggttt
     7981 catttagctt tggatttgtt tcaaaattaa agtatgccac attttcatag tggagacgtc
     8041 caaattccaa aatggaataa gtttttccaa cctgtcttgc accctgtaaa attaaaggtt
     8101 tgcgatgatt actttctttc cattcttcca agaaattcat tatttttcta tacataagtt
     8161 acctccttat tgaaaggata acaaatattc gtgtattttt caatgtattt tattgcaatt
     8221 ttaacactat tttacacgta tattttgtaa ataatcacat taaatgacac gtactaagaa
     8281 aaaggagttg aaatccatca actcctaagt gttttattta tcgaatttaa tagcagcctg
     8341 tgcagcagca agtctagcga ttggtacacg atatggtgaa caagaaacgt agttcaatcc
     8401 aactctatga cagaattcaa tacttgctgg atcaccacca tgttctccac aaatacctaa
     8461 tttaatattt ggacgagtgc ttcttccaag ttcagtagcc atcttaacaa gttttccaac
     8521 acctctttga tctaagtgtg caaatggatc ttgttcgaaa atcttcttgt catagtaatc
     8581 ttgtaagaat cctcccgcat catctcttga gaagccaaat gtcatctgcg tcaagtcatt
     8641 tgtaccaaat gagaagaatt cagcttcttt tgcgatttca tccgccaata aggcagcacg
     8701 aggaatttca atcattgtac ctacatgata tttcatatct aattcggatt cttgaatcaa
     8761 tttgtcagct acatcacata caaccttttt aacataacga agctctgcaa catcaccaac
     8821 taatgggatc ataatttctg gttcaatctt ccagtctggg tgtctttcat taacagctaa
     8881 agcagcctga atcactgccg ttgtctgcat ttcagcaatt tctggataag aaatagccaa
     8941 acgacatcca cgatgaccca tcatagggtt gaattcgtgt aagctagaaa taacagattt
     9001 caattcatcc aaaccaatgt gcatatcttt agctaattgt gtaatatcat aagagtttgt
     9061 tggcaagaat tcatgtaaag gtggatccaa gtaacgaatt gtcactggta atccttgcat
     9121 agcttcatag attccttcaa agtcacttct ttgcataggc aagatttttt ctaatgcgac
     9181 acgtctttga gctgccgttt tagaaacaat catttcacga actgctttaa tacgatcacc
     9241 ttcaaagaac atgtgttccg tacgaactaa accaatacct tgcgcaccaa attcatgtgc
     9301 ttgttttgca tcatgtggtg tatctgcatt tgtacgaact tttaatgtac gtctttcatc
     9361 tgcccagttc ataaaacgtt caaagtcacc tgaaatactt gcaggaacgg ttttaatcgc
     9421 actaccataa atatttcctg ttgacccatc taaactgatc caatctccac gatgatattt
     9481 cacaccatca atttcaaaat atccttcatc atcatgcata ttgatatcgc cacaaccact
     9541 tacgcagcaa gtacccatac cacgagctac cacagcagca tgacttgtca taccaccacg
     9601 taccgttaag ataccttgag atacgtgcat accttcaata tcttctggac ttgtttctaa
     9661 acgaactaag attactttgt ggttacgact tgcttcttgg attgcttctt cggcactaaa
     9721 tacgatttgt ccacaagcag caccaggact tgctggaagt gctgaagcaa ttggttcagc
     9781 tttctttaat tcatccgcat caaacattgg gtgaagtaat gaatccaatt gttttggttc
     9841 taccatcatt aaagcttcat ccgtagtaat cataccttca tccaccatgt cgcacgcaat
     9901 ctttaatgca gcttgtgctg tacgtttacc attacgtgtc tgcaacatga acaatttacc
     9961 tttttcaatt gtgaattcca tatcttgcat attgcgatag tgagtttcca attttttagt
    10021 aatcgcaaca aagtcatcat atgctccagg agctacttct tttaattgat cgattttttg
    10081 aggtgtacga atacccgcaa caacatcttc accttgtgca ttcattaaga attcaccata
    10141 taatttattc tcaccagtac ttggattacg cgtaaaggca acacccgtac cacaatcatt
    10201 acccatatta ccaaatacca tcatctgtac gttaactgca gttccccagc ttgatggaat
    10261 atcattcatc ttacgataat agatagcacg aggattgttc caagaacgga atacagcctc
    10321 aatagcgccc atcaattgtt ctttaggttc ttcagggaat tctgatttta attcattttt
    10381 gtagaatgcc ttaaacaatt ctaccaattt tttcatatcc tctgcattta aatctaaatc
    10441 atccgtaatg cctctttcag cctttacttc atcaatgatc tcttcaaaac gctttttact
    10501 caaccccatt acaacatctg aatacatttg aataaaacga cgatatgaat catatgcgaa
    10561 acgctcatta ttagttaagt tagccacagt ttctgcaact gttttgttca tacctaaatt
    10621 taaaatagta tccatcatac caggcatact tgcacgtgcc cctgaacgaa cagatactaa
    10681 taaaggattg gcttcatccc ctaatttctt tccagatttc ttttctaata aatctagata
    10741 ctccataatt tgagctttaa taccatcatt gatagtttca ttatcttcat aatattggtt
    10801 acaagcttct gtagtaattg tgaatccata aggtacaggc attccaagtt tagacattcc
    10861 agctaaatta gcaccttttc cacctaacaa ttcacgcatt gtttcatcgc cttcattaaa
    10921 ttcatagaca tattttttat ccataatact ttacctcctt ttgtaaccgt ttacatgtgt
    10981 aaactattat aacatacaaa attacataat gatagttttt tttaaatttt cacaaataaa
    11041 aacctatcag ttttcaccaa taggtttatc cacgcttaac aaccgcactt ttttgtctaa
    11101 acgcaatacc aatctttaca atatttttaa ctccaaaatc ctttaattct gtatcatatt
    11161 tcatatcatc aatctgtcta agtgcactat tagccaatga atctaaatca acatttatat
    11221 cttttgtatg tttaaactca aagataaatc caggtattcc ctgcatcatc ggcaataatt
    11281 caacatcaaa ccgacctagt cctgattccc tatttgatct taccttatat tgacttccta
    11341 gtacagcaca taatccaagc atcattctgt gatagaatgc ttcattagcc ccatccatcg
    11401 ttgatatact ttttaacata aaatcctcca acaaagactg cagtttacgt gtatctttag
    11461 aaaagatagc ctgatgaatt gaaattgaaa cattattttg atttgtgcga ttcagcactt
    11521 ctttttcata tacatataaa atttctttat taggaatagc cacatcacac atataatttc
    11581 catcattttg cggatatatc gccgccacct ttagataacc cgcaaccaat aagaaactat
    11641 atatactata aggattgttt tgaacctcag gatatattac acttgtatct acataagttg
    11701 tgattgtatt tccacaaaat aatttatata aattctcagt aatttcaggt gttgcagttc
    11761 caataatttc accaataata tcattactac ccgttgattg ccaaaaagct tttggaaaac
    11821 attgatccga aatataattg atcacagacc aaggattaaa tatctcggta
//
LOCUS       DBMDWL010000055        11848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1839_length_11848_cov_3.448200, whole genome shotgun sequence.
ACCESSION   DBMDWL010000055 DBMDWL010000000
VERSION     DBMDWL010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_11848_cov_3.448200"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            103..516
                     /locus_tag="ACZPDN_05230"
     CDS             103..516
                     /locus_tag="ACZPDN_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HIR1298057.1"
                     /translation="MSAFDKLDQFKLFMFDTGLLKQMAGVDNSAILLKTDYQFKGPMT
                     ENYVLQQLKGQFEVEPRYYSDKNGEIDFVVQNKMEIIPIEVKGGEDKSANSFKTYVAN
                     NSPQHAYRFSKRGYRKDGDFTNLPLYLVRKTKDLL"
     gene            complement(534..887)
                     /locus_tag="ACZPDN_05235"
     CDS             complement(534..887)
                     /locus_tag="ACZPDN_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298058.1"
                     /translation="MKTWKLVSGIISIVLSVFVVFQSMMAGLANALEESGQSSGSAGL
                     VVSICMLCGGIVSIVSRKNNSKGTNIALIILFGLATFTGFVLAGNYSDLYIWSFWCLI
                     NTILAIISSRKGIRN"
     gene            complement(1141..1788)
                     /locus_tag="ACZPDN_05240"
     CDS             complement(1141..1788)
                     /locus_tag="ACZPDN_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR1298059.1"
                     /translation="MKSVLIIGMGRFGRHMAQKLYDLGHDVLAIDKSEAKINKVLSYV
                     TNAQIGDSTDESFVDSLGVSNFDLCVVAIGDDFQSSLETTALLKDYGAKLVVARAVRD
                     VHAKFLLRNGADEVVYPEKQIGNWAAVRFSSENIFDYVQLTPDYSIYEISVPNSWIGK
                     SMLELDIRRKYHINILATKINGILDPLPSAEHTFEESENILILAQNSDLQKFLRF"
     gene            complement(1799..3136)
                     /locus_tag="ACZPDN_05245"
     CDS             complement(1799..3136)
                     /locus_tag="ACZPDN_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1298060.1"
                     /translation="MFKKRMNRITPGQMIILSFATMIFIGACLLMLPFSTNDGRGAPF
                     LDALFTSTSASCVTGLIVHDTAQYWSPFGQLVILTLIQTGGMGVITMAIILSVLGGKK
                     IGFKQRYFMQQSISAPKLGGIIRNTKWILKATLCVEALGAILLGIRFLPRFGLVKGIW
                     YSIFHSISAFCNAGFDLMGTKQAYSSLTSYYGDILISNVIAALIVLGGLGFFVWKDVV
                     QYKRHLHKYSLQSKVVLFTTAALLIISTLYFYINDFSHWNMSLADQINVSIFQAVSPR
                     TAGFNTIDLNKMNQSSILFMTLLMLIGGSPQGTAGGFKTTTLAVLILSIRAVFNHKQN
                     PQCFGRRISIDSLNNATALFMLFMVLFFTGALLISAFDQLPILDALFEAASAIGTVGL
                     TLGITPTLSSASHCILIFLMFFGRVGGLTLLLAISKHQVVANANLPQENITIG"
     gene            3282..5135
                     /locus_tag="ACZPDN_05250"
     CDS             3282..5135
                     /locus_tag="ACZPDN_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR1298061.1"
                     /translation="MASVLLCLSTSKYNSRVIEKGAQIAKNNHATLSAVYVQTPKDES
                     MSAASKSCLRENIKFAESKGAKVTILYGHNKIRQIVEYVDVSQVDCVVIEKSLASQAL
                     FRLRDIDVYSVNYPHDYRRLFYFDFSSFRFSFKDLLKMILILILCTLIGKGFMHFQFL
                     ITTPIMIYILGIVFVSIWTSGYIYSLLASLFSVLCFNFFFTYPYFSLLSDPSYITTYI
                     VMFVVAMSSSSLMTRLKKQSFENAKQVYRTQVLLEMSQMLQKANDMNAIYASMLKQLH
                     KLLDTDLVLYPHNDEPILLGQCPIETNIVAWVYENKHEAGKTTSYHSDSMCLYLPING
                     THEVLGVVGIVLKDKMDSFEKNLWLAILDECGMALEKERIRLENLKIEQQAKQEALRA
                     DLLRMISHDLRTPLTSISGNAGLLLENENAFSLEKKKELYQDIYNDAMWLYDLVENLL
                     FITRIENGTMQLNLQPEMIEDIFREAIHHLGRNKRNHNISMHLEDDLLMANMDARLII
                     QVLVNLMNNAIKYTPENSKIVLSAKRIENKILIEVQDDGNGVLHPDQLFEMFYTENNH
                     SGDTRRGMGLGLSLCKSIVLAHGGKIWVENVNPHGACFKFLLDAVEVNLYE"
     gene            5128..5841
                     /locus_tag="ACZPDN_05255"
     CDS             5128..5841
                     /locus_tag="ACZPDN_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1298062.1"
                     /translation="MNKVQILVIEDDVAVGNLITTTLEMENYQFRLAKTAKQGIMNVL
                     SYNPDIMLLDLGLPDMDGLEVIKKVRSWSNIPIIVVSARSEDQDKVQALDLGADDYLT
                     KPFSVDELLARLRVTIRRLNNSQTISVNESVYENGDLTINYAQGCVYENGHEIHLTPI
                     EYKLLCVLAKNTDKVLTHNYIMQEVWGSVQGTETPSLRVFMATLRKKIEPDTSNPKYI
                     QTHVGVGYRMLNQTEKKED"
     gene            6304..6597
                     /locus_tag="ACZPDN_05260"
     CDS             6304..6597
                     /locus_tag="ACZPDN_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1298063.1"
                     /translation="MGDCCHKKHQPRDEKEIKQLTNRLHRMVGQLNGIENMLKENRYC
                     GDILVQVAAVESALQAFGYIVLQEHLNTCVVEDVQNGNTEIMSEVMDLVKKLK"
     gene            6607..9315
                     /locus_tag="ACZPDN_05265"
     CDS             6607..9315
                     /locus_tag="ACZPDN_05265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1298064.1"
                     /translation="MEEKFDVTGMTCAACQANVTRVVSKLDGVSHCDVSLLSNSMKVE
                     FDQDKVNEQVICEAVKQIGYGASVHGEKGEVKKEWQNRQNRVESDQRSAKQRLILSLV
                     LLIPLLVIAMGPMVGLPILEGMEWMMVSALTQLILCLFILFIQRHFFITGFKALMKKS
                     ANMDSLVAMGSGASFVYGLVGIYQMAYYFGVQNIEMAHMAMHSLYFESAATIVTLVSV
                     GKYLESRSKAKTNDALDKLVDLAPKNATILRDGKEVVVSSESIRIHDIVVIRPGQRIP
                     VDGKVVSGTGYVDQSAITGESLPVEKNVGDSVISATMNENGTFQFEAEKVGQDTTLAQ
                     IIQLVDDAGNSKAPIARLADKVSGVFVPVVILIALITFVAWLACGQTIQFALSNAISV
                     LVISCPCALGLATPVAIMVGTGKAAENGILIKSAAILEQLHSIDTIVLDKTGTITSGK
                     PSVQGIKVYTNISENDFISMAASLESGSLHPLGQAIVEYAKDKNMNLEQPENFTNISG
                     RGIQAKLNGHVYLAGNKRLVEENKIELNETVLNDLDAYASLGQTPLIFVEDQNVIGLI
                     SVADTVRSTSQVALEQFKQKNMHVVMLTGDNQKTAEAIGKSLSVDEVISDVLPQDKES
                     VIRKLQEQGKKVMMVGDGINDAPALMRADIGVAIGAGTDIALDSADVILMKSSLLDVV
                     TAIDLSSDVIKNIKMNLFWAFFYNILGIPVAAGLLYPAFGLRLSPMIGSACMSLSSVC
                     VVTNALRLRYFKPRIEMEEVKTFTMHIDGMSCGHCAWLVEDALKKIPNVKEVRVDYNL
                     GKADIDYVQQVNMVALKQAVKDAGYIPVEYKEEKKMKKIVTVDGMMCMHCVAHVKDAL
                     SKVEGVSDVVVSLDEKTATLNCTENVTDQMLSDAVTNAGYTVISVK"
     gene            9395..9550
                     /locus_tag="ACZPDN_05270"
     CDS             9395..9550
                     /locus_tag="ACZPDN_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298065.1"
                     /translation="MECFIDGKSTCERTFWNRLNVLANFQQKEMIMDGLKVRVAESVY
                     WIQPKKG"
     gene            complement(10266..10652)
                     /gene="mutT"
                     /locus_tag="ACZPDN_05275"
     CDS             complement(10266..10652)
                     /gene="mutT"
                     /locus_tag="ACZPDN_05275"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HIR1298066.1"
                     /translation="MKTIHVVAAIIKDGNKIFTTQRGYGEFKDGWEFPGGKVEPNETS
                     RQAIVREIKEELDTTIQVEEYFDTVEYDYPNFHLSMECFICTILEGNLTLLEHESAKW
                     LTKETLDSVDWLPADLGLIAKLKERL"
     gene            complement(10657..>11043)
                     /locus_tag="ACZPDN_05280"
     CDS             complement(10657..>11043)
                     /locus_tag="ACZPDN_05280"
                     /inference="COORDINATES: protein motif:HMM:NF023335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HIR1298067.1"
                     /translation="KYDQTTKTFPVFINYDKANDIQDTIKYEDHFINESQLIAISKSG
                     RTTSSDDVQNFLYAKERGIEVHLFVRKNKDDKTSKEFYYLGNMQAEIGKTKQFIMPNT
                     QKTAVEILWQLDTPVREDIYRYIVEE"
     assembly_gap    11044..11053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11054..>11848)
                     /locus_tag="ACZPDN_05285"
     CDS             complement(<11054..>11848)
                     /locus_tag="ACZPDN_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HIR1298068.1"
                     /translation="GNYKNNFMIPIALSGDRSYNKDNIRRYVLEGERIIPGSSTIHFD
                     EISKKKIFASIDTANFADIKLIKDSYKNLKNKLGRIPTLMDFETYGEIDVQRIFDNKS
                     LGSYYAFLKKYEKDYKVRLNGLEESFIKFICTKLANGKRKIELETLNDLIDDRDYIFP
                     NDISQTNVFNIFTNEFPTGSGKNTFKDCIFIEDTKISKPFKTCLENNEFKSMVKEIIE
                     YALDQYNKHFKNTYKDTNFVLYQKYTYEDACRLLNWQHNEVPLNIG"
BASE COUNT         3734 a         1645 c         2455 g         4004 t
ORIGIN      
        1 cagtatcatc aaattgctgt ccttgcggtt tattatagct ggtagtgtat aagcgtgggt
       61 tcatcatatt tggtgtggta tctttaacta tgggaaactc cattgtctgc ttttgataaa
      121 ttggatcaat ttaaattgtt tatgtttgat actgggcttc ttaaacaaat ggcgggtgtg
      181 gataatagtg ccatattatt gaaaacggat taccaattta aaggaccaat gacagaaaac
      241 tatgttttac aacaattaaa aggacagttt gaagtagaac cacgatacta ttcggataaa
      301 aatggtgaga ttgattttgt ggttcaaaat aaaatggaaa ttattccaat tgaagtaaag
      361 ggtggggagg ataagtctgc caattccttt aagacatatg tcgcaaataa ttcacctcaa
      421 catgcatatc gtttttctaa acgtgggtat cgtaaggatg gtgattttac aaatcttccg
      481 ttatacttgg ttcgaaaaac aaaggattta ttataattta aaaggtatca gttttaattt
      541 ctgatacctt ttcttgatga aatgattgcg agtattgtat tgattaagca ccaaaaagac
      601 catatatata ggtctgaata gttacctgca agtacaaagc ctgtgaatgt agctaaacca
      661 aaaagaataa taagtgcaat gtttgtgcct ttagagttgt tctttctaga tacaatcgat
      721 acaattcctc cacaaagcat gcatatacta acaacaagtc ctgcactgcc actggattgt
      781 ccactttctt ctagtgcgtt ggcaaggcct gccatcatac tttggaatac gacaaataca
      841 cttaatacaa tggaaatgat tccactgact aatttccatg ttttcataat gataaaatcc
      901 cctttcttaa gtatatatat aacttagaaa tggggataat tggtatgctt gtggcataca
      961 ttaatggtgt gtgatatcta agatgtgatt taaaagtcgt gggactaaga aagaaatgta
     1021 gattacggat cctattgata aaatacctaa cataaataat cgcctctttc ttacaccttt
     1081 attgtagaat tttcaatgtt taaatggtgt taaagaatga ggcgacatat taagatttca
     1141 ttaaaagcga aggaattttt gtaggtctga attttgtgca agaatcaaaa tgttttccga
     1201 ttcttcaaaa gtgtgttccg cactaggtag tgggtcaaga attccattga ttttagtggc
     1261 taaaatattg atgtggtact ttcttcgaat atcaagttct aacatacttt ttcctatcca
     1321 tgaatttggc acagaaattt cataaattga gtagtctgga gtaagttgta catagtcaaa
     1381 gatattttca cttgagaaac gtacggctgc ccaattacca atttgttttt caggatatac
     1441 gacttcatcg gctccattac gcaataagaa tttagcgtgc acatcacgta cggctcttgc
     1501 gacaacaagt ttagcgccat aatcttttaa taaggcggtt gtttctagtg agctttgaaa
     1561 gtcatctcca atggctacaa cgcaaaggtc aaaattggat acgcctagtg agtcaacaaa
     1621 actttcgtcg gtggaatctc caatttgtgc atttgtgaca taagataata ctttgttaat
     1681 tttagcttca cttttatcaa tagctaaaac gtcatgtcct aaatcatata gtttttgtgc
     1741 catgtgacgt ccaaaacgtc ccattccaat aattaatact gatttcatat tttctccttt
     1801 atccaattgt gatattttct tgtggtaaat ttgcgtttgc gacaacttga tgtttagaaa
     1861 tagctaacag taaagttaat cctccaactc ttccaaagaa catcaagaaa attaaaatgc
     1921 agtgggatgc acttgatagt gtgggtgtga ttcctaatgt aagtccaaca gttccaatgg
     1981 cagaggccgc ttcaaatagg gcgtctagaa tgggtagttg atcgaaggct gaaataagga
     2041 gtgcgcctgt aaagaataat accataaata acataaataa ggctgtggca ttgtttaatg
     2101 agtctatgga aatgcgtctt ccgaaacatt gtgggttttg tttatgattg aatacagcac
     2161 gaatacttag tatcaatacg gcaagtgttg tagttttaaa acctccggct gtaccttgtg
     2221 gacttcctcc tatcaacata agtagtgtca taaagaggat gctgctttga ttcattttgt
     2281 ttaaatcaat cgtattaaag ccggctgttc ttggggatac ggcttggaag attgaaacat
     2341 tgatttgatc ggctaagctc atattccaat ggctaaagtc attgatgtaa aagtagagcg
     2401 tactaataat caataaagcg gctgtcgtaa atagcacgac tttagattgt aaggaatatt
     2461 tgtgcaaatg acgtttgtat tgaaccacat ctttccagac aaagaaacct aagccgccaa
     2521 gtacaattag tgctgcgata acattggata taagtatgtc gccataatag ctagttaggg
     2581 atgaataggc ttgttttgtg cccattaagt caaatccagc attacagaag gcggaaatag
     2641 aatggaatat ggaataccat atgcctttga ctaggccaaa gcgaggcaga aagcgaatgc
     2701 ctaataaaat ggctcctaat gcttctacac ataaggtagc tttaagaatc cattttgtgt
     2761 ttcgaataat accgcctagt tttggtgcgg aaatagattg ttgcataaag tatctttgtt
     2821 taaaaccaat ttttttacca cctagaacag ataggattat tgccatagta ataacgccca
     2881 tacctccagt ttgaataagt gttaaaatga caagttgtcc aaatggggac caatattgag
     2941 ctgtatcatg cacaatcagt ccggttacac atgaggcact ggttgaagtg aataatgcat
     3001 ctaaaaaggg agcgcccctt ccatcgtttg tggaaaatgg aagcataagc agacaggcac
     3061 caataaatat catagtcgca aaactgagga tgatcatttg tcctggggta atgcgattca
     3121 tacgtttctt aaacataaaa ccacctatcc taatatctca acacattcta cactagtctg
     3181 cattaagatt ttataaaaat attctaacac tatattaaga caatgttaag ataattggtt
     3241 ctttgtgggc ttgttttgtg ggcttgtgtt atacttgtcg tatggctagt gtattattat
     3301 gtttatcgac ttcaaaatat aattcaagag tgattgaaaa aggggctcaa attgcgaaga
     3361 acaatcatgc gacgttaagt gctgtttatg tgcagacccc aaaagatgaa agtatgagtg
     3421 cggcatcaaa gagttgtttg cgtgaaaata ttaagtttgc ggagagtaag ggagctaaag
     3481 ttacaattct ttatgggcac aataagattc gacagattgt tgaatatgtg gatgtttcac
     3541 aagtggattg cgtagttata gaaaagagtc ttgcctcaca ggctttattt agattgcgtg
     3601 acattgatgt atattctgtg aattatcctc atgactatcg tcgtttgttt tattttgact
     3661 ttagttcttt tcgtttttcc tttaaagatt tgttaaagat gattttgatt cttattcttt
     3721 gcacgctaat tggcaaggga tttatgcact ttcaattctt gattacgaca cccattatga
     3781 tttatatctt ggggattgta tttgtatcta tttggacaag cggatatatc tatagtttat
     3841 tggcatcttt attttcagta ttatgtttta actttttctt tacatacccc tatttttctt
     3901 tgttgagtga tccaagctat attacgacat atattgtgat gtttgtggtg gctatgtctt
     3961 caagttcatt aatgacaagg ttaaagaaac agtcttttga gaatgccaaa caagtatatc
     4021 gtacgcaagt cttattggag atgagccaga tgcttcaaaa ggcgaatgat atgaatgcga
     4081 tttatgcgtc catgttaaaa cagctgcata aattgttgga tacagattta gttttgtatc
     4141 cacataatga tgagcctatt ttacttggac aatgtccgat cgagacaaat attgtggctt
     4201 gggtatatga aaataaacat gaagctggta aaacaacaag ttatcattcg gattcgatgt
     4261 gtttatattt acctataaat ggaacgcacg aagtattagg tgtggttggg attgttttaa
     4321 aagataagat ggattctttt gaaaagaatt tgtggcttgc gatattggat gagtgtggta
     4381 tggctttaga aaaggaaagg attcgtctag aaaatctaaa gattgagcag caagctaaac
     4441 aagaagcgtt gcgagccgat ttattgcgta tgatttcgca tgatttaaga acgccattaa
     4501 catctatatc tggtaatgca ggtttattgt tggaaaacga gaatgcgttt tctttagaaa
     4561 agaagaagga attgtatcag gatatttata atgatgcgat gtggctttat gatttggtgg
     4621 aaaacttatt gtttataacg agaattgaaa atggtacgat gcagttgaac ctacaaccag
     4681 aaatgattga ggatattttt agagaggcca ttcatcattt ggggcgcaat aagcgtaatc
     4741 ataatatttc tatgcattta gaggatgatt tgttgatggc caatatggat gcgcgtttga
     4801 ttattcaagt attagtgaat ttaatgaata atgcgattaa atatacgcca gagaattcta
     4861 aaattgtttt gagcgcaaaa cgaattgaga ataagatatt aattgaagtt caagatgatg
     4921 gaaatggtgt tttacatcca gatcaactgt ttgaaatgtt ctatacggaa aataatcata
     4981 gtggagatac aagacgaggt atgggtttag gtttatcttt gtgtaagtcg attgttttag
     5041 cgcatggtgg taagatttgg gtagaaaatg taaatcctca tggtgcttgt tttaaatttt
     5101 tattggacgc agtggaggtg aatctttatg aataaagtac aaatattagt tattgaagat
     5161 gatgttgcgg taggaaattt gattacgaca acacttgaga tggaaaacta tcagtttcgt
     5221 ctggcaaaaa cagcgaaaca aggtattatg aatgttcttt cttataatcc tgacattatg
     5281 cttctagact taggtttacc ggatatggat ggtttggaag ttataaagaa ggttcgttct
     5341 tggtcaaata ttccaattat tgtggtttcg gcaagaagtg aagatcaaga taaagtacaa
     5401 gctttagatt taggggccga tgactatttg acaaaaccat tttcagtgga tgaattgttg
     5461 gctagacttc gtgtaacgat tcgaagatta aataatagtc agaccataag tgtaaatgag
     5521 agtgtatatg aaaatggaga tttgacaatc aattatgcgc agggttgtgt atatgagaat
     5581 gggcatgaaa tccatttaac gccgattgaa tataaattat tgtgtgtttt agctaaaaat
     5641 actgataagg ttttaacaca taattatatc atgcaagaag tatggggaag tgttcaaggt
     5701 acagaaacgc cgagtctaag agtgtttatg gctactttgc gtaaaaagat tgagccggat
     5761 acttcaaatc cgaaatatat tcagacgcat gtgggtgttg gatatcgtat gttgaatcag
     5821 acagaaaaaa aggaagacta gtgtcttcgc atattgaaat agagtgaaat aataattgtg
     5881 ataactccaa ttgtgatgag tgccataatg gttttattga tgcatagaag gatcaatata
     5941 ctaaataggc tgacaatcgc aaataggaag atttcaattt ttaatagaat gggatacatc
     6001 aacaatgaat cgcgaaaact aattttttcg tattgtggtt cattgttttt taatagtctt
     6061 ttttcatata taaatagaat ttgactaaag agcacgacaa aaacgaggta tatgagatta
     6121 aaaaagattt cttttgagaa gagtgcaccg cacgttaaaa acatgacaag aatggttcca
     6181 attcttggca gatacacgta ataattatat ttcatattgt tttttccttt cttttattat
     6241 gccataatca gattacttga aatatacctg tagggggtat atcattgtat cggggtgaaa
     6301 tatatgggag attgttgtca taaaaagcat cagcctagag atgagaaaga aatcaaacaa
     6361 ttgacgaatc gtcttcatcg tatggtagga caattaaatg gaattgaaaa tatgttaaag
     6421 gagaatcggt attgcggcga tatcttggtg caggtagcgg cagtagagag tgctttgcag
     6481 gcatttggat atattgtttt gcaggaacac ttgaatacgt gtgtcgttga ggatgtgcaa
     6541 aatggaaata cagaaattat gagtgaggtt atggatttag taaagaagtt aaagtaggtg
     6601 gttttaatgg aagaaaaatt tgatgtgaca ggcatgactt gtgcagcttg tcaggctaat
     6661 gtaactcgtg ttgtgagtaa attagatggt gtgagtcatt gtgatgtttc tttattatcg
     6721 aattcaatga aggttgaatt tgatcaagat aaggtgaatg aacaagtgat ttgtgaagct
     6781 gtaaaacaaa ttggctatgg agctagtgtt catggtgaaa agggtgaagt taaaaaggaa
     6841 tggcagaatc gtcagaaccg tgttgaatcg gatcaaagaa gtgctaaaca aagattgatt
     6901 ttaagtttag ttttattgat tccattactt gtgattgcga tgggacctat ggtaggtctt
     6961 ccgattctag agggtatgga atggatgatg gtttccgctt taacacagtt gattctatgt
     7021 ctatttatat tatttattca aagacatttc tttattacgg gatttaaagc tttaatgaaa
     7081 aagtcggcca atatggattc tttagttgcg atgggttcag gtgcaagctt tgtgtatggc
     7141 ttagtcggca tttatcaaat ggcttattat tttggtgtac agaatataga aatggcgcat
     7201 atggctatgc actctttgta ttttgagtca gctgcaacaa ttgtgacttt ggtcagcgtc
     7261 ggaaagtatt tagagtctag atccaaggca aaaacgaatg atgcgctaga taaattggtg
     7321 gatttggcgc caaagaatgc gacgatttta agggatggta aagaagtcgt tgtttcaagt
     7381 gagagtattc gaattcatga tatagttgtg attcgtccag gacaaagaat tcctgtggat
     7441 ggtaaagttg tttctggtac aggttatgtg gatcagtcgg cgattacggg tgagagtctt
     7501 ccggtagaaa agaatgtagg ggattctgtt atttcagcta ccatgaatga gaatggcact
     7561 tttcagtttg aggctgaaaa ggtaggacaa gatacaacgt tagctcagat tattcagcta
     7621 gttgatgatg caggaaattc gaaggcacct attgctcgtt tggcagataa agtcagtggt
     7681 gtatttgttc cagttgtgat tttgatcgca ttgattacat ttgttgcttg gttagcttgt
     7741 ggtcaaacca ttcaatttgc gctttccaat gcgattagtg tattggttat ttcttgtcct
     7801 tgtgcattgg gcttagctac gccagttgcg attatggtag gtacaggtaa ggctgcggag
     7861 aatggtatct taattaagtc ggctgctata ttagaacagc tacattcgat tgatacgatt
     7921 gtattggata agacgggtac aattacttca ggaaagccaa gtgtgcaagg cattaaggtt
     7981 tatacaaata taagcgaaaa tgattttatt tcaatggcgg caagcttaga gagtggttct
     8041 ttacatcctt taggacaggc tattgtggaa tatgcgaaag ataaaaatat gaatctagaa
     8101 caacctgaaa acttcacaaa tatttcgggt cgtggtattc aggctaagct aaatggtcat
     8161 gtgtatttgg ctggaaataa gagattggtt gaagaaaata aaattgaact caacgaaaca
     8221 gtattaaatg atttagatgc ttatgcaagt ttaggtcaaa caccattgat ttttgtggaa
     8281 gatcaaaatg tgattggctt aattagtgtg gcggatacag tgcgtagcac aagtcaggta
     8341 gcattagagc agtttaaaca aaaaaatatg catgtggtta tgttgactgg agataatcaa
     8401 aagacggctg aagcaatagg taaatcttta agtgtggatg aagtcatcag tgatgtgtta
     8461 ccacaagata aagagagtgt gattcgtaag cttcaagaac aaggtaagaa ggttatgatg
     8521 gttggtgatg gaattaatga tgcgcctgct ttaatgcgtg ctgatattgg agttgcgatt
     8581 ggtgctggaa ccgatattgc cttagatagt gcggatgtga ttttgatgaa gagttcatta
     8641 ttagatgtag tgacggcaat tgatttatca agtgacgtta ttaaaaatat taagatgaat
     8701 ctattttggg ctttcttcta taacatttta ggaatcccag ttgcggcagg tttattgtat
     8761 ccagcatttg ggcttcgatt atcaccaatg attggttcgg catgtatgtc tttgagtagt
     8821 gtttgtgttg tcacaaacgc tttacgactt cgttatttta aacctagaat tgaaatggaa
     8881 gaagtcaaaa cattcacaat gcatattgat ggtatgtctt gtggacattg tgcatggttg
     8941 gttgaggatg ctttaaagaa aattccaaat gtaaaagagg ttcgtgtgga ttataatttg
     9001 ggtaaggccg atattgatta tgtgcaacaa gtaaatatgg tggctttaaa acaagcagtt
     9061 aaagatgcgg gttatattcc cgttgaatat aaggaggaaa agaaaatgaa aaaaatagta
     9121 acagttgatg gtatgatgtg catgcattgt gtggcacatg taaaggatgc attaagtaaa
     9181 gtggaaggtg taagtgatgt agtggttagt ttagatgaaa aaacagctac attaaattgt
     9241 acagagaatg tcacagatca gatgttgagt gatgccgtga caaatgcagg atatacagtc
     9301 atttctgtga aataaagaat cataaaaatt aaaaaagtca agacttgtaa gcggattcag
     9361 aatattctat gatagacgtc gagggagacg caaaatggaa tgttttattg atggaaaatc
     9421 cacttgcgaa agaactttct ggaatcgttt gaatgttttg gcaaactttc aacaaaaaga
     9481 aatgataatg gatggtttga aagttcgtgt tgcagaaagt gtatattgga ttcaaccaaa
     9541 gaaaggatag ttcgtttgaa ttattctttt ttttgacagt taaattaagt gaaagccttg
     9601 atgtatgttt tatataaagt gaaaatagat gtttttaaaa ctaattaaat gttgtatgga
     9661 attttaattg atttgaaatt tatttgacag ttgaatcaca tgaaaatctc aatatttcct
     9721 ttaaataaag taataataga tgcttttgac attgataaaa tgttgtatgg gatttttagc
     9781 ttttcttgtg gaaatacatt cggtttcttt gtataataca tatagagaac gactactgga
     9841 aaagcagtgg ttactctgaa ataaaattaa tgctgaagca ctaatctttt tccattgtca
     9901 gtggtgatta gtgctttttg catttttcta taattatcaa tgttactttt agtatttcta
     9961 ctatggcaaa taacacaaaa gcaacatcaa tatgtagata tatatacata agctttcctc
    10021 ctgtaaaaaa tagaaagatc tgcttacctg gaactcaaga ttcaaggctt caaatctcct
    10081 taacaagagg taaccaccaa ccacatccag tagccaattt caattataaa acttcaatgt
    10141 gaaaacattg tgaattccta taaatttgtt gattatttga cagttggata aaagaaaaac
    10201 gagcatttgt tctttatgta aagatgaaat gctcgttttt aggcatttta aaatgttgta
    10261 tggaattata atctttcttt taatttggcg attaatccta aatctgcagg tagccaatcg
    10321 actgaatcta atgtttcttt agtaagccat tttgcagatt catgttctaa tagagttaaa
    10381 tttccttcaa ggatcgtaca aatgaaacat tccatagata ggtggaagtt tggatagtca
    10441 tattcgacgg tatcaaagta ttcttcgact tgaatggttg tatctaattc ttctttgatt
    10501 tcacgaacaa tcgcttgtct acttgtttca ttcggttcta cttttccgcc tggaaattcc
    10561 catccgtctt tgaattcgcc atagcctctt tgggtggtga atattttatt accatcttta
    10621 ataatggcgg ctactacgtg tatcgttttc aaaaaatcac tcctctacaa tatatcgata
    10681 aatgtcttct cttacgggtg tatctaattg ccataagatt tcaacagctg ttttttgtgt
    10741 gtttggcatt ataaactgtt ttgtcttacc aatttctgct tgcatgttac ctaaataata
    10801 gaattcttta cttgttttat catctttgtt ttttcgcaca aacaaatgaa cttcaatacc
    10861 tctttctttg gcatataaga agttttgtac gtcatcacta ctagtggttc ttccggattt
    10921 ggatatcgca atcaattgac tttcattgat aaaatgatct tcatatttga tggtatcttg
    10981 aatgtcattt gccttgtcat aattgataaa gacagggaat gttttggttg tttgatcata
    11041 tttnnnnnnn nnncgccaat atttaatgga acttcattgt gttgccaatt taataagcga
    11101 catgcatctt catatgtata tttttggtag agtacaaagt ttgtatcttt atatgtattc
    11161 ttaaaatgtt tattatattg atccaatgca tattcaatga tttcttttac catggattta
    11221 aattcgttgt tttctagaca tgtcttaaat ggttttgaaa ttttcgtatc ttcaataaaa
    11281 atacaatctt taaaagtatt ctttccagat cccgttggaa attcgttggt gaagatattg
    11341 aatacatttg tttgagatat gtcatttggg aatatataat ctctatcatc gattaaatcg
    11401 tttaatgttt cgagttcaat ctttcttttt ccattggcta gttttgtaca aataaattta
    11461 ataaaacttt cttcaagtcc attcaatcgt actttataat ccttttcgta tttctttaaa
    11521 aaggcatagt aagatcctaa tgatttgttg tcgaaaattc tttgtacgtc aatttctcca
    11581 taagtttcaa aatccattaa ggtaggtatt cttccaagtt tattctttag atttttatag
    11641 ctatccttaa tgagtttgat atccgcaaag ttggctgtat caatcgacgc aaagattttt
    11701 tttttactga tttcatcaaa gtgaatcgta cttgatcctg gaatgattct ttcaccttct
    11761 aatacatagc gtcttatatt gtctttattg taagaacgat ctccagatag ggcgattgga
    11821 atcataaagt tgtttttgta gtttccaa
//
LOCUS       DBMDWL010000056        11656 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1886_length_11656_cov_3.018693, whole genome shotgun sequence.
ACCESSION   DBMDWL010000056 DBMDWL010000000
VERSION     DBMDWL010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1886_length_11656_cov_3.018693"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            156..1523
                     /gene="dnaA"
                     /locus_tag="ACZPDN_05290"
     CDS             156..1523
                     /gene="dnaA"
                     /locus_tag="ACZPDN_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR1298069.1"
                     /translation="MSTMSYASIWNETLQKIQESNYFDTHTFNSWISKTTLFKIEDNI
                     AYVAYRSTITFSILKKQKEKELFESTLSEVWGDTLTIQYIHQREMEKMMPEEVVKQRT
                     NVLLENKFNPSYTFENFVEGNSNAEAYAACLACCTQTTSHLFNPLMLYGNSGLGKTHL
                     LHAMGNYLAKEHPECKVIYMYSGDLVSLLIEAMKTKNVHGNTVERVKEQLLDCDYFLI
                     DDIQNLQQHSSSQEIFFTVYNQLIQKNTQIIITSDMHPSEISGLQSRLISRFVSGLTI
                     SISKPEFETSKAILKKKIEGREEGVNMSEDVIDYLAGKYSNDVRNLEGTLNRLIFNAT
                     LFNPDVIDMDFARKVLIKEPIVSQSDELTIKKIKKAVTRFYGLSYKDLEGKCRQKFIA
                     NARHICVYLSRDLLHKSYVSIGQELGGRDHTTISSSYERAKKLIQKDEAFKTAVEKIR
                     STLDS"
     gene            1669..2787
                     /gene="dnaN"
                     /locus_tag="ACZPDN_05295"
     CDS             1669..2787
                     /gene="dnaN"
                     /locus_tag="ACZPDN_05295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR1298070.1"
                     /translation="MHFSIDRKYFYDKLSIVSRAISVFSPLPALSGICIDLKDDSMTL
                     TGSDSNISIRTVIVPGELNNLDIEEEGSIIIDSKYLLEIVRKLDCKNIEIEVIDYSLV
                     RISSDNGQFNLNGIRSNEYPDIDFTQPNHHFVLKATDLKSIVSQTAFACSDKDQRPVL
                     TGVNFNSQGNMLYCSGTDSYRLARKTIELGSSQDFNITIPSKSLTEVVRSVSDDDTID
                     IFADSKKAQFVFDKTIIQTRLIDGTFPDVQRIIPTSCVSKMLVDSYEIAGTIDRTNFI
                     RNDKVHLIKLECTPQETHVKTYSSEIGNSDEVLTKCEYEGEEISLTCNGTYMLDAIKA
                     LGCEKVLIEFSGFMKPIKVSNPEDASVLMILVPIRSYD"
     gene            2902..3105
                     /gene="yaaA"
                     /locus_tag="ACZPDN_05300"
     CDS             2902..3105
                     /gene="yaaA"
                     /locus_tag="ACZPDN_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HIR1298071.1"
                     /translation="MEFELRDEYITLAQLLKACDVVYSGGQAKEYLASMEVFVNGVNE
                     NRRGKKIYHGDIVETNGIRIEVK"
     gene            3102..4193
                     /gene="recF"
                     /locus_tag="ACZPDN_05305"
     CDS             3102..4193
                     /gene="recF"
                     /locus_tag="ACZPDN_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865687.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1298072.1"
                     /translation="MNVEKLTCFHFRNYETMSFSFVPKCIHFLYGKNAQGKTNLIEAI
                     YFLSHLRSFRTNQMDSMIMHGCNEFCVQAIVESNHRKEELKVIVDHQKKHLFRFQNPV
                     KKYSDFIGIENAILFCPDDLSLFTSSPKNRRRFIDMELMKLSKTYTSTLSSYQKLLKQ
                     RNQALKQSNIDECLVQIYLDQMIEVQSVIIKQRNEFLNSLMNKARELYPFFSNEKEEI
                     GAKYMTFIPIDPDMKSHMKEAYDKVFEKEKRYHQTLIGIHRDDILFELNENPVCEVAS
                     QGQKRSFVLALKLGLAQIIYEKSGQYPILLLDDVFSELDDFRKRQLIEKLPRDMQIFI
                     TTTERLNINWNREVRFYDIEKGRIKEVLK"
     gene            4190..6157
                     /gene="gyrB"
                     /locus_tag="ACZPDN_05310"
     CDS             4190..6157
                     /gene="gyrB"
                     /locus_tag="ACZPDN_05310"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR1298073.1"
                     /translation="MSQDELQKEILEFEELESQATQVEHSYTADDIQVLEGLEAVRLR
                     PGMYIGSTSARGLHHLVWEIVDNGIDEALAGFANRIDVTIEPDNIIAVRDNGRGMPVG
                     IHEKTGISAVETIMTVLHAGGKFGGGAYKVSGGLHGVGASVVNALSEWLEVTVFQDGK
                     IYFIRFENGGHAVEPLKVIGETTETGTLVRFKADSQIFKDTTIYDYETLNSRLRQLAF
                     LNKDIRLTLTDKRTPDGLSNDYKYEGGIIEYVQFLNKNKSTISDKVIYVEGLQDGILA
                     EVALQYNDGYQSSIYSFCNNIHTHEGGYHEDGFRMALTRCINTYAKNNNMIKKDETLS
                     QDDVKEGLVAIISVKHPDPQYEGQTKTKLGNSEVRKIVSNIAGEQINRFFLENPDIAK
                     AIVEKAEIASKARIAAKKARELTRRKSALDGISSLPGKLTDCSSKDASECEIYIVEGD
                     SAGGSAKQGRDAKTQAILPLRGKILNVEKARTHRIFENQEIRSMITAFGCGIQEDVDV
                     SKLRYHKIVIMTDADVDGSHICTLMLTFLYRFMKPVIEGGYVYIAQPPLYKVQKGKKI
                     AYAYKEQEMDQLREEFKDGYKVQRYKGLGEMDAEQLWETTMDPHHRVLKRVTIDDAME
                     ADSVFDMLMGEDVEPRREYIQENAVYANLDY"
     gene            6170..8638
                     /gene="gyrA"
                     /locus_tag="ACZPDN_05315"
     CDS             6170..8638
                     /gene="gyrA"
                     /locus_tag="ACZPDN_05315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1298074.1"
                     /translation="MEEENKRYDYVEDVEISKEMRTAFLDYSMSVIVSRALPDVRDGM
                     KPVHRRILHAMNQLGITSGVAHKKSARIVGEVIGKYHPHGDTAVYDAMVRMAQDFSYR
                     YPLVDGHGNFGSLDGDGAAAMRYTEARMSKISMEMMRDIQKDTVDFIPNYDGEENEPV
                     VLPSRIPNLLINGAVGIAVGMATNIPPHNLSETIQAIFAVMDDPDITIPQLMEVIKGP
                     DFPTGGIILGRKGIRQAFETGRGSIMIRSKYRVEELTNGKKQIIFYEIPYQVNKANLI
                     TKMASLIRDKAIQGVTYLNDESNREGIRIVMELKKDAQEEVILNQLFRLTPLQTSFGI
                     NMLALENGRPKQLPLKDIIHDYIDHQVDVVVRKTQFELKKAQDRAHILEGLRIAMDHI
                     DEVIHMIRSSKKDEAGLSQDLCDAFGLSMIQAKAILAMQLRRLSGLERDKIENEYQQL
                     LLTIEDLKDILANHDRVLQIIRDDLNEIDQKYGDERRTEISDASVDMEDEDLIPVEDV
                     IITLTESGYIKRQLVDTYRAQNRGGRGIKSLTLNEEDSIDTMISMSTHDFLLLFTDKG
                     RVYRLKGYNVPSGSRTSKGIPIVNLMTLEKGEKVNVLVAVPKDDESETLLFVTKNGIV
                     KRTSVSEFENINRNGKIAISLKPDDELRFVKLTTGQDEVIISGSNGKAVRFNEDQVRI
                     MGRSASGVLGFNCDGSEVVGAALSSEGDTVLVVSENGYGKRSKFEDYRLTSRGKKGVS
                     TINITEKTGKLMCMRAVTGKEDAMIVASDGIMIRVALENVGIYGRNTQGVRLINLNGD
                     AKVTRMTLVDHEEPEVESEENTEE"
     gene            8783..9367
                     /locus_tag="ACZPDN_05320"
     CDS             8783..9367
                     /locus_tag="ACZPDN_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1298075.1"
                     /translation="MNAKDMGLFICKLRKENNMTQMELAQQLHVTDKAVSRWERGIGY
                     PDIQLLPALSESLHVSVAELISCKKSSNYSNEEVTNIIHNLDVYKMESFRQDRKADKI
                     SLICMIFVAACVYISGHGSIIGSLCIGAIFAMAVIGLYYLYYDNSTKRIYAFFSLLGL
                     LLFIQFLIFMGMDYRWISYILTLYIFVILNMICR"
     misc_binding    9455..9665
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9705..10988
                     /gene="serS"
                     /locus_tag="ACZPDN_05325"
     CDS             9705..10988
                     /gene="serS"
                     /locus_tag="ACZPDN_05325"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR1298076.1"
                     /translation="MLDMKFVRENPDIVKENIKKKFQDYKLPLVNEVLMEDKELRQTQ
                     QHADDLRANRKKISKEIGKLMGQGKKEEAEEIKQQMAEIAHELTALEEKEIKLRESVT
                     EKMMQIPNIIDDSVPIGKDDSENVELERFGEPVVPNFEIPYHTEIMERLSGIDLDAAR
                     RVAGNGFYYLMGDIARLHSAILNYARDFMIDKKGFTFVIPPYMIRSSVVDGVMSFAEM
                     EGMMYKIEGEDLYLIGTSEHSMIGKFKDTILEEKDLPYTYTSYSPCFRKEKGAHGIEE
                     RGVYRIHQFEKQEMIVVCKPEDSMEWFKKLYMNTVEFFRTLDIPVRTLECCSGDLADL
                     KCKSVDVEAWSPRQKKYFEVGSCSNLTDAQARRLKIRVKGENGEKYYAHTLNNTCVAP
                     PRMLIAFLENNLQEDGSILIPEALQPYMGGKKKIG"
     gene            11054..11139
                     /gene="ffs"
                     /locus_tag="ACZPDN_05330"
     ncRNA           11054..11139
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZPDN_05330"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            11187..>11656
                     /gene="tadA"
                     /locus_tag="ACZPDN_05335"
     CDS             11187..>11656
                     /gene="tadA"
                     /locus_tag="ACZPDN_05335"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR1298077.1"
                     /translation="MNMDDVKWMKEAIKQAKKAESYDEVPIGCVIVKDDKIIARGYNK
                     RETLQQSIAHAEIMAIQKACKKLGTWRLEDCVLYVTLEPCPMCAGAIIQSRIKEVVYG
                     ASDPKGGCVGTCTNLFEVSEFNHHPIYRRGILESECSDLLKHFFKKKRDMKKKQ"
BASE COUNT         4015 a         1496 c         2387 g         3758 t
ORIGIN      
        1 tctatgtatt taacatgaat ataaagtgag ttatcccaat gtgattcatt ttttttacac
       61 atttatccac atgtgcatat tttatatccc caaatattaa atggggataa gtgtaaaaat
      121 cgtgtataat aatactactg caaggaggtt ttactatgtc taccatgtct tatgcatcga
      181 tatggaatga aactttacaa aaaattcaag aatccaacta ttttgatact catacattta
      241 attcatggat ttctaaaacc actctattta aaatagaaga taatatagct tatgtagctt
      301 atcgttcgac cattactttc agtattttga agaaacaaaa agaaaaagag ctttttgaat
      361 ctacactgag tgaagtttgg ggagatactt taaccattca atatattcat caaagagaaa
      421 tggaaaagat gatgcctgaa gaggttgtta aacaaagaac gaatgtttta ttagaaaaca
      481 aatttaatcc gtcatataca tttgaaaact ttgttgaagg aaattcgaat gcggaagctt
      541 atgctgcatg tttagcgtgt tgtactcaaa caacttcaca tctttttaat ccgcttatgt
      601 tatatggaaa ttctggctta ggtaaaacac atttacttca tgcgatggga aattatttag
      661 ctaaggaaca tcctgagtgt aaagtcatct atatgtatag tggggatttg gtttccttat
      721 taatagaggc catgaagact aaaaacgtcc atggaaatac tgtggaaaga gtcaaagaac
      781 agcttttaga ttgtgattat tttttaattg atgatattca aaacttgcaa cagcattcta
      841 gcagccaaga aatctttttt accgtataca atcaattgat tcaaaaaaat acacagatca
      901 ttatcacaag tgatatgcat cctagtgaaa tcagtggttt acagtctcga ttaatttcac
      961 gatttgtttc cggattaaca atcagcattt ctaagcctga attcgaaacg agtaaagcta
     1021 ttttaaaaaa gaagatagaa ggtcgcgaag aaggtgtgaa tatgagtgag gatgttattg
     1081 attatttagc tggtaaatat tcaaatgatg tcagaaatct agaaggtacc ttaaatcgct
     1141 tgatttttaa tgctacttta ttcaatccag atgtaatcga tatggatttt gcacgaaaag
     1201 tactaattaa agagcctatt gtatcgcaat cggatgaatt aacaattaaa aaaattaaaa
     1261 aagccgttac acgcttttat ggtttatctt ataaggatct agagggtaaa tgtcgtcaaa
     1321 aatttattgc gaatgctcga cacatctgtg tttacttatc tagagacttg cttcataaat
     1381 cttatgtatc gattgggcaa gaattaggag ggcgtgatca tacgacaatt tctagttcgt
     1441 atgaacgtgc taaaaaattg attcaaaaag atgaagcttt taagacggct gtagaaaaaa
     1501 tacgtagtac tttggattct tgatttatac actgtattcc tcatggaatc cacatccaaa
     1561 aattgacaga aaagcatgta ttttaaaggg tctgtgcatt ttttacacac ttatacagtc
     1621 gcttatatta taattaatat ataaatatat taataaaagg aggattttat gcatttttca
     1681 attgatcgaa aatattttta tgacaaacta tctatagttt ctagagctat tagtgttttt
     1741 tctccactac ctgcattatc aggtatctgt atagatttaa aagatgattc tatgacttta
     1801 acaggtagtg attccaatat atctattcgt actgtaattg ttcctggtga attgaataat
     1861 ttagatattg aagaagaagg ttcaattatt attgattcta aatacttatt agaaattgtt
     1921 cgtaaattag attgtaaaaa cattgaaatt gaagtcattg attattcttt agtgcgaatt
     1981 tcaagtgaca atggacaatt taatttaaat ggtattcgtt ccaatgaata tccagatatt
     2041 gattttacac aaccaaacca tcattttgtt ctaaaagcaa cagatttaaa atctattgtt
     2101 tctcagactg cctttgcgtg cagcgataaa gatcaacgtc ctgttttaac aggtgttaat
     2161 tttaattctc aaggtaatat gttgtattgt tcaggaacag attcttatcg tttagcaaga
     2221 aagacaattg aattaggttc aagtcaagat tttaatatta ccattcctag taaatcttta
     2281 acggaagtgg tccgttccgt ttcagatgat gatactattg atatttttgc agattctaaa
     2341 aaagctcaat ttgtatttga taaaacaatc attcaaactc gtttgattga tggaactttc
     2401 ccagatgtac aaagaatcat tccaacaagc tgtgtttcga agatgttagt agactcatat
     2461 gaaattgcag gtacaattga tcgtacaaac tttattcgta atgataaggt acatttaatt
     2521 aaattagaat gtaccccaca agaaactcat gtgaagacat actctagtga aattggtaat
     2581 tctgatgaag tgttaacaaa atgtgagtat gagggagaag aaatctcatt aacatgtaat
     2641 ggaacttata tgttggatgc aatcaaagct ttaggttgtg aaaaagtatt gattgaattt
     2701 tctggtttta tgaagccaat caaggtatct aaccctgaag atgcgtcagt gttaatgatt
     2761 ttggtgccaa ttcgtagtta tgattaaaga aaatgccgtt taaacttgtt ttaaacggtt
     2821 tctttttata tcggtatctc tgtacgcata gatatttatg aggttttatg atataattag
     2881 agtgcgaaaa gaggtatttt catggaattt gaattaagag atgagtatat tacattagca
     2941 caattgctga aagcttgtga cgttgtttat agcggcggac aggctaagga atatttagca
     3001 agcatggaag tattcgtaaa cggcgttaat gaaaatcgtc gtggaaagaa aatctaccat
     3061 ggagatattg tagaaactaa tggaataagg atcgaagtga aatgaatgtt gaaaaactga
     3121 catgttttca ttttcgtaat tatgaaacca tgtctttttc ttttgtacca aaatgcattc
     3181 attttttata tggaaaaaat gcacaaggaa aaacaaattt aattgaggct atctattttt
     3241 taagtcattt gcgttcgttt cgcacaaatc aaatggacag tatgatcatg catggatgta
     3301 atgaattttg tgttcaagca atcgtagagt ctaatcacag aaaagaagaa ttgaaagtga
     3361 ttgtggatca tcaaaaaaag catttatttc gttttcaaaa tccggttaaa aaatattcag
     3421 attttattgg gattgaaaat gccatcttgt tttgtcctga tgatttatct ttatttactt
     3481 catctccaaa aaatcgaaga cgatttattg atatggaatt gatgaagtta tcaaaaactt
     3541 atacatctac tttgtcttct taccaaaaac ttttaaaaca aagaaatcaa gctttgaagc
     3601 aaagtaatat agacgaatgt ttagtacaga tttatttaga ccagatgata gaagttcaaa
     3661 gtgtgattat caaacaaaga aatgaatttc taaattcttt gatgaataaa gcacgtgagc
     3721 tttatccttt cttttcaaat gaaaaagaag aaataggtgc aaaatatatg acttttattc
     3781 ctattgatcc ggatatgaaa agtcatatga aagaagcgta cgataaggtt tttgaaaaag
     3841 aaaaaagata tcatcagact ttgattggaa ttcatagaga tgatattttg tttgaattaa
     3901 atgaaaatcc tgtatgtgaa gtcgcaagtc agggacaaaa aagaagtttt gtattggcac
     3961 ttaagttggg gttggctcaa attatttatg aaaagagcgg gcaatatcca atattgttgt
     4021 tggatgatgt atttagtgaa ctggatgatt ttagaaagcg tcagctaatt gaaaagcttc
     4081 ctagagatat gcaaattttt ataacaacaa cagagagatt aaatataaat tggaatcgtg
     4141 aagtgcgatt ctatgatata gaaaaaggga gaatcaagga ggtcttaaaa tgagtcagga
     4201 tgaattacaa aaagagattc ttgaatttga agaattagaa tcacaagcta cacaagtaga
     4261 acattcgtat actgcggatg atattcaagt gttggaaggg ttagaagcgg ttcgtttaag
     4321 accaggaatg tatattggtt cgacttcggc tcgtggactc caccatttag tttgggaaat
     4381 cgtagataat ggtattgatg aggcattagc tggatttgca aatcgaattg atgtaacaat
     4441 tgagccagat aacattattg cggtacgtga taatgggcgt ggtatgccgg ttggaattca
     4501 tgaaaagacg ggaattagtg cagttgaaac cattatgaca gttttacatg ctggtggtaa
     4561 atttggtggg ggcgcatata aggtttccgg aggtttacat ggtgttggtg ctagtgttgt
     4621 taacgcattg agtgaatggt tagaggttac tgtatttcaa gatggaaaaa tttattttat
     4681 tcgttttgaa aatggtggtc atgcggttga accattaaaa gttattggtg aaacaactga
     4741 aacgggtact ttggttcgtt ttaaggcgga ttcgcagatt tttaaagata caactattta
     4801 tgattatgaa acattaaatt ctcgtttgcg tcagcttgca ttcttaaata aagatattcg
     4861 tttaacttta acagataaaa gaacgccaga tggtttaagc aatgactata agtatgaagg
     4921 tggaattatt gagtatgtac aattcttaaa taagaataag agtacaatca gcgataaagt
     4981 tatttatgta gaaggattgc aggatggtat acttgcagaa gtagccttgc agtataatga
     5041 tggctatcaa tcaagtattt attcattctg taataatatt catacgcatg aaggtggata
     5101 tcatgaagat ggttttagaa tggctttgac tcgttgtatc aacacatatg cgaaaaacaa
     5161 caacatgatc aaaaaggatg agactttatc tcaggatgat gttaaggaag gtttggttgc
     5221 gattatttct gtaaagcatc cagatcctca atatgaagga cagacaaaga caaaattagg
     5281 aaatagtgaa gttcgtaaga ttgtttctaa tattgctgga gaacaaatca atcgtttctt
     5341 cttggaaaat ccagatattg ccaaggctat tgtggaaaaa gcagagattg ccagcaaagc
     5401 aagaattgcg gctaaaaaag ctcgtgaatt aacacgtcgt aaatctgcat tggatggtat
     5461 tagttcatta ccgggtaagt taacggattg ttcaagtaaa gatgcaagtg aatgtgaaat
     5521 ctatattgtc gaaggggatt ctgccggggg cagcgcgaaa caaggtcgtg acgcgaaaac
     5581 acaagcaatt ctacctttgc gtggtaagat tttaaatgtt gaaaaagcta gaacacatcg
     5641 tatttttgaa aaccaggaaa tccgtagtat gattactgct tttggatgtg gtattcaaga
     5701 agatgtagat gtatctaaat tacgttatca caagattgtt attatgaccg atgccgatgt
     5761 tgatggtagt catatttgta cattgatgtt gacgttctta tatcgtttta tgaagcctgt
     5821 tattgaaggt ggatatgttt atattgcaca accgccttta tacaaggtgc aaaaaggtaa
     5881 aaagattgcc tatgcatata aagagcagga aatggatcag ttgcgtgaag aatttaaaga
     5941 tggatacaaa gttcagcgtt ataaaggttt aggtgaaatg gatgcagaac agttgtggga
     6001 aacaactatg gatccacatc atagagtatt gaaacgtgtc actattgatg atgcgatgga
     6061 agcggacagt gtatttgata tgttgatggg tgaggatgta gagcctcgtc gtgaatatat
     6121 tcaagaaaat gctgtatacg ctaatttgga ttactagtag gaggtcctta tggaagaaga
     6181 aaataagcga tatgactatg tagaagatgt tgaaatatcc aaagaaatgc gtacagcttt
     6241 tctagattat tcaatgtccg ttattgtttc acgtgcatta ccagatgttc gtgatggtat
     6301 gaaacctgtt catcgtcgta ttttacatgc gatgaatcaa ttgggaatta cttcaggtgt
     6361 tgcacataaa aaaagtgctc gtattgttgg tgaagttatt ggtaagtatc accctcatgg
     6421 tgatacagct gtatatgatg caatggttcg tatggcccaa gatttctctt atcgatatcc
     6481 actagtggat ggacatggaa attttggttc tctagatgga gatggagctg cggcaatgcg
     6541 ttatacggaa gctcgtatgt ctaaaatttc tatggaaatg atgcgtgata ttcaaaagga
     6601 tacggttgat tttattccaa actatgatgg tgaagaaaat gagccggttg tgcttccaag
     6661 tcgtattcca aacttattga tcaatggggc tgttggtatt gcggttggta tggcaacaaa
     6721 tattccacca cataacttat cagaaacaat tcaagctatt tttgcggtaa tggatgatcc
     6781 agatattaca attcctcaat tgatggaagt cattaaagga cctgatttcc ctactggtgg
     6841 tatcatttta ggccgtaaag gaattcgtca agcatttgaa acaggtcgag gatcaattat
     6901 gattcgttcg aagtatcgtg tagaagaatt aacaaatgga aagaaacaga ttattttcta
     6961 tgaaattcct tatcaggtta ataaggcaaa cttaattact aagatggctt ctttaattcg
     7021 tgataaagct attcaggggg ttacttattt aaatgatgaa agtaatcgtg aaggtattcg
     7081 tatcgtcatg gaattaaaga aggatgcaca ggaagaagta attttaaatc aattgtttag
     7141 attaactcct cttcagactt catttggtat caatatgttg gcacttgaaa atgggcgccc
     7201 taaacaatta ccattaaaag atattattca tgattatatt gatcatcagg ttgatgtagt
     7261 tgttagaaag actcaatttg agttaaagaa agcacaagat cgtgcacata ttttagaagg
     7321 tttacgtatt gcgatggatc atattgatga agttattcat atgattcgca gttctaaaaa
     7381 agatgaggca ggcttatctc aagatttatg tgatgcgttt ggattgagta tgattcaggc
     7441 aaaagcaatc ttggctatgc aattaagaag attatctgga ttagaaagag acaagattga
     7501 aaatgaatat caacaattat tattaacgat tgaagattta aaggatatct tggcaaatca
     7561 tgatcgtgtt cttcaaatca ttcgtgatga tctaaatgaa attgatcaga aatatggcga
     7621 tgaaagaaga actgaaatta gtgatgcttc tgttgatatg gaagatgaag acttaattcc
     7681 agttgaagat gtaattatca cattaactga atcaggatat attaagcgtc aacttgtaga
     7741 tacatatcgt gctcaaaatc gtggtggacg cggtattaaa tcattaacgt taaatgaaga
     7801 agattctatc gatacaatga tttcaatgtc aacacatgat ttcttattgt tgtttacaga
     7861 taagggtcgt gtatatcgat taaaaggata caatgttcca agcggatcta gaacatctaa
     7921 aggaattcca attgtgaatt taatgacttt ggaaaaaggt gaaaaagtaa atgttcttgt
     7981 agctgttcct aaggatgatg aaagtgaaac attattattt gttacaaaga atggtattgt
     8041 aaaacgtaca tccgtatctg aatttgaaaa tatcaatcgt aatggtaaga ttgcgattag
     8101 tttaaaacca gatgatgaat tacgttttgt gaaattaact acaggtcaag atgaagtaat
     8161 tatttctgga tcaaatggta aagctgttcg atttaatgaa gatcaagttc gtatcatggg
     8221 acgtagcgca agtggtgtat taggattcaa ttgtgacggc agtgaagttg taggtgctgc
     8281 actctcaagt gaaggagaca cagttcttgt tgtttctgaa aatggttatg gtaaacgtag
     8341 taagttcgag gattatcgtt tgacttctcg tggtaaaaaa ggtgttagca caattaatat
     8401 cacagaaaag actggtaagc taatgtgtat gcgtgcggtt actggtaaag aagatgcgat
     8461 gattgttgcc agtgatggaa tcatgattcg tgtggcttta gagaatgtag gaatctatgg
     8521 aagaaataca caaggtgtaa gattgatcaa cttaaatggg gacgcgaaag taacgcgtat
     8581 gacattggta gatcatgaag agcctgaagt agaatccgaa gaaaatacag aagaataatg
     8641 catgtaagga gtagaattat aattttactc ctttttcata tgacaaatca gatggaaaca
     8701 acccgaatac attatataaa cgacacgttg attgccgatt tgatagaatg gatataaatt
     8761 attatagaaa gaagtgatat atgtgaacgc aaaagatatg ggattgttta tctgtaagtt
     8821 aagaaaagaa aacaatatga cacaaatgga attagctcaa cagctacatg tcacagataa
     8881 agctgttagc cgatgggaac gtgggatagg atatccagac attcaattgt tacctgccct
     8941 atctgaatct ttgcatgttt ctgtggctga attgatatca tgtaaaaaga gttcaaatta
     9001 ttcaaatgaa gaagtcacaa atattattca caatttagat gtatacaaaa tggaaagttt
     9061 tagacaagat agaaaagcag ataaaatttc cttaatatgt atgatctttg ttgcggcatg
     9121 tgtttatatt tctggtcatg gaagtattat tggttctttg tgtattggcg ctatttttgc
     9181 aatggcagtg attggcttat attatttgta ttatgataat tcaacaaaac gaatttatgc
     9241 cttcttttct ctattagggt tacttttgtt tattcagttt ttaatattca tgggaatgga
     9301 ttatagatgg atttcttata ttttaacact ttatattttt gtgatattga atatgatttg
     9361 tagataagtt gaatcatatg aaaaatctga tttgatgagg ttaattcaca gaataagagg
     9421 ttgactttag ctttatttta atggtataaa tgtgttgaaa acaaagataa ggataagtag
     9481 attatttcat ttatgaatta gagacagtat ggttggtgaa aatactgata tgaatagttg
     9541 aatagcacct tagagtgatt gaatgaaatg agtagtttga tccggtacat accgttatca
     9601 tgttaagaga tcatatatgt atatatatgg taaaaagggt ggcaacgcga gacatcgtcc
     9661 ctttatgggt gcgggtgtct ctttttttat gcaaggagga tatcatgcta gatatgaaat
     9721 ttgtccgtga aaacccggac attgtcaaag agaacattaa gaaaaagttc caagattaca
     9781 aattgccttt agttaatgaa gtgttgatgg aggataaaga acttcgtcaa acacaacagc
     9841 atgccgatga tttacgtgcg aatcgtaaaa agatttctaa agaaatcggt aagttaatgg
     9901 ggcaaggaaa aaaagaagag gctgaagaaa ttaagcaaca aatggctgaa atcgctcatg
     9961 agctaacagc tttagaagaa aaagaaatca aattaagaga gtctgtaaca gaaaaaatga
    10021 tgcagattcc aaacatcatt gatgatagtg ttccgattgg aaaagacgat agtgaaaatg
    10081 ttgaattaga aagattcggc gaacctgttg ttcctaattt tgaaattcct tatcacactg
    10141 aaatcatgga acgtttaagt ggaattgatt tggatgcagc acgtcgtgta gctggtaatg
    10201 gattctacta tttaatggga gatattgctc gtctacattc agcaatctta aattatgctc
    10261 gtgatttcat gattgataaa aaaggattta catttgttat tccaccatat atgattcgta
    10321 gttcagttgt tgatggtgta atgtcatttg cagaaatgga aggcatgatg tataaaatcg
    10381 aaggtgaaga tttgtatttg attggtacaa gtgaacactc tatgattggt aaattcaaag
    10441 acacaattct tgaagaaaaa gatcttccat atacatatac ttcatactca ccttgtttcc
    10501 gtaaagaaaa aggtgctcac ggtattgaag aacgtggtgt atatcgtatt caccaatttg
    10561 aaaaacaaga aatgattgtt gtttgtaaac ctgaagatag tatggaatgg tttaaaaaat
    10621 tgtatatgaa tactgtagag ttcttcagaa cattagatat tccagtacgt actttggaat
    10681 gctgttcagg tgatttggca gatttaaaat gcaagtcagt ggatgttgaa gcttggtctc
    10741 cacgtcaaaa gaaatatttt gaagtcggaa gttgctctaa cttgacagat gctcaggcac
    10801 gtcgtttaaa gattcgtgtt aagggtgaaa atggagaaaa atattatgca cacacattaa
    10861 acaacacatg tgttgctcca ccaagaatgt tgattgcatt cttagaaaat aatttacaag
    10921 aagatggatc tatcttgatc ccagaagctt tacaacctta catgggtggt aaaaagaaaa
    10981 ttggttaatt atgaaggctg tcatttgaca gctttttcta ttgcgaattg aaaatggatt
    11041 tgatataata acctagctac tacaatgata tcttgtgaat caggtcaggt cgggaacgaa
    11101 gcagccttaa gcaagctcca tcatgtgtag tagttttttt atattttgtt taaatttcat
    11161 gtatgattaa tatataggaa gaggatatga atatggatga tgtgaaatgg atgaaggaag
    11221 ctatcaagca ggcaaaaaaa gcagaaagct atgatgaggt tcctattggt tgcgtgattg
    11281 taaaagatga taaaattatt gctcgaggat ataataagcg agagactttg caacaaagta
    11341 ttgcacatgc agaaattatg gctattcaaa aagcttgtaa aaaattaggt acatggagat
    11401 tggaagattg tgttttgtat gtaactttag aaccatgtcc tatgtgtgca ggtgccatta
    11461 tccaatctag aatcaaagaa gttgtttatg gagcatcaga tcctaaagga ggttgtgtag
    11521 gcacatgtac aaatttattc gaagtatctg aatttaatca tcatcctata tatagaagag
    11581 gcattctaga atctgaatgt tcagatttat tgaaacattt ttttaagaag aaaagagata
    11641 tgaaaaagaa gcagaa
//
LOCUS       DBMDWL010000057        11317 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1972_length_11317_cov_2.461662, whole genome shotgun sequence.
ACCESSION   DBMDWL010000057 DBMDWL010000000
VERSION     DBMDWL010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1972_length_11317_cov_2.461662"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            309..533
                     /locus_tag="ACZPDN_05340"
     CDS             309..533
                     /locus_tag="ACZPDN_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1490 domain-containing protein"
                     /protein_id="HIR1298078.1"
                     /translation="MKDGWKNKTMWTFIGGAATALAGSKFLKSDTAHDLAVKGLAGGM
                     KFKSDTTCKYETIKEDAKDLLEEAKVKNEG"
     gene            540..2642
                     /locus_tag="ACZPDN_05345"
     CDS             540..2642
                     /locus_tag="ACZPDN_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1298079.1"
                     /translation="MISFTIAHDIPGRMRIRYGKDVFSPVEGEVLKRDLSNWTMIHSV
                     EVNSITGSVLMQYDATCKDTLLKKLHNLNLGYLKEADTSDIVFRSQTPEVKAMNAEYK
                     RRFFKIIGKRYFIRWFLPTGIGNAITIYKSLGFIKEGLKSLSQMKVNVPLLDATSIGV
                     SLLQKNFNIAGNIMMLLNISDLLEDYTRKKTNLELSQSLSIQFDKVWIYADGVEQEIP
                     MAKLQKGDIVVSQMGAMIPIDGEVVDGEAMVNEASFTGEPLSRRVVKEDTVFAGTLIE
                     EGKLFIKVRNLQDESRISNIVKMIDTNESLKASIQAKAEHLADSIVPFSFFGFFGILA
                     LTRDVTRATSVLMVDYSCAIRLSTSISVISAMLEASNRNVLVKGGKYLEAVKDADVIV
                     FDKTGTLTNANPQVQKVTPLNGYSRDEVLRIAACLEEHFPHSVANAIVNQAKKEGLVH
                     EEEHAKVEYIVAHGIATTLHGLHTVIGSDHFIFEDDGVEKTQELEDIITSLEKDGAGS
                     LIYLAIDNQVAGIISIYDPLKQEAKQVIHELKNLGIQKVVMLTGDCNNAAKAVANELD
                     LDDYRSSILPEGKASYIQSLKDEGHTVIMVGDGINDTPALSTANVSISMQDSSDLARE
                     LADVTLVSSNLNELVTLRKLSVSLFDRIHANYRFIVSFNSSLIALGALGFMSPNTSSL
                     LHNGSTFAIAATSTRKYL"
     gene            2701..3213
                     /locus_tag="ACZPDN_05350"
     CDS             2701..3213
                     /locus_tag="ACZPDN_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298080.1"
                     /translation="MVQKLNHSELKDITDIVYYTNLKQKNLVLRNTLVLGYTIAGSLL
                     CWVVGASLIRVASGASVFLFLCSLICLCVVIYILICRFLEKRRCRKRVQEKETGEFNV
                     HFRKDYMLYDQQTFSLKRFKKVYLYHDLYYFVDSENVVIVKKCQEVDRAIHKTPYLKV
                     QKKDALFCLL"
     gene            complement(3229..3588)
                     /locus_tag="ACZPDN_05355"
     CDS             complement(3229..3588)
                     /locus_tag="ACZPDN_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR1298081.1"
                     /translation="MNLPTIIICVILIIIICIGIRSTKKRATSGCCGSSETVKKIRIK
                     DKNKEHYPYKVVLKVYDIHCQNCVHTIENAFNSQEGFYAKVNTEDNTVTILSKQEHTT
                     DELIDIVTKAGYTSKAV"
     gene            3733..3942
                     /locus_tag="ACZPDN_05360"
     CDS             3733..3942
                     /locus_tag="ACZPDN_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1298082.1"
                     /translation="MPLSFADLNSVNTIKKIGGSKEQQRHLENLGFVPGTVIVVVSKT
                     NGNVIVNVKEVRIAISEELARKIMV"
     gene            3955..4173
                     /locus_tag="ACZPDN_05365"
     CDS             3955..4173
                     /locus_tag="ACZPDN_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1298083.1"
                     /translation="MTLNKVSIGQTVTVKKLSGQGATKRRIMDMGITKGVSVTVQKVA
                     PLGDPIELTVRGYQLSLRKADAEMIEVE"
     gene            4196..6322
                     /gene="feoB"
                     /locus_tag="ACZPDN_05370"
     CDS             4196..6322
                     /gene="feoB"
                     /locus_tag="ACZPDN_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1298084.1"
                     /translation="MSIQVALAGNPNCGKTTLFNGLTGATQYVGNWPGVTVEKKEGKY
                     KEDKDIKITDLPGIYSLSPYTLEEVVSREFLLNGNVDVVLNIIDGSNLERNLFLTTQI
                     LELGIPTVVAINMLDVIEKRKDTIDYKKLSEELGCPVLPISALKNTGIKELMAQVKKA
                     AGTKFSAKNIYAGKVLNALNTIETSLPSSIEADRRFFYAVKLFERDDKIEAAIKSKAD
                     ASVIEACEKSMDDDSESIITDARYTYITSVIKDCYKKGSKEVLTTSDKIDRIVTNRVL
                     ALPIFALVMWFVYYISVTTVGTIVTDWTNDVLFGEIIPPAVDSFLDAIQCADWLHGLI
                     VDGIIGGVGAVIGFVPQMLVLFFFLAILEDCGYMARVAFIMDRIFRKFGLSGKSFIPM
                     LIGTGCGVPAVMASRTIESDRDRKMTIMTTTFIPCGAKMPIIGLIAGAIFHGASWVAP
                     SAYFVGMAAVIISGIMLKKTKLFAGDPAPFVMEMPAYHTPRAVNVLRSMWERGSSFIK
                     KAGTIILLSTIVLWFLQGFGWEKGAFGMVDDIDHSILSSIGQTFAWIFSPLGWGDWKA
                     AVASVTGLIAKENVVATFGQLYGFAEVAEEGNEFWGQLSASFTPLAAYSFMVFNLLCA
                     PCFAAMGAIKREMNNTKWFWIAIGYQCGFAYVCSLIVYQLGSLVNGGSFGFGAIVGFI
                     LLIGLIYLLVRPSKESDTAEVEFAVK"
     gene            6341..6535
                     /locus_tag="ACZPDN_05375"
     CDS             6341..6535
                     /locus_tag="ACZPDN_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIR1298085.1"
                     /translation="MNLSTFLVLLVVCGCAGLACRSIYKDKKSGKSSCGGSCGHCGSH
                     SMCENPKAFFDQIKKEQFSK"
     gene            6595..7824
                     /locus_tag="ACZPDN_05380"
     CDS             6595..7824
                     /locus_tag="ACZPDN_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1298086.1"
                     /translation="MNLKEKYADLIVRSGLNVQKDQIVVVRANVEMNEFVRLIVKSCF
                     EVGAKDVIVRYSDDEINRMRYLSGNRMPYDYEALFHNETAEKGACYLSLVGEDPDGMK
                     GVDPKLMSNFSKTLRGMTQPYRDKLDYMHAAWCVASVPTMAWAKKVYPESEDALSDLW
                     NAVLKMSRVNEKDANENWNEHRASFDKRVHILNHLDIESVHYTNSLGTDLIVELPEGY
                     VFAGGGSFLDNGNYYFPNIPTEEIFSAPKKTGVNGKLYSALPLAYNGAVVKDFWFEFK
                     DGQVVDYDAKEGKEVLTSILETDEGSKYLGEIALVPYGSPISAMDTLFYETLIDENAS
                     CHFALGASYNECIENGLNMSEEELLEHGMNQSFAHVDFMVGTSDLSIEATLKKGEKIF
                     IFKDGKYTREFDGYTLN"
     gene            7852..9117
                     /locus_tag="ACZPDN_05385"
     CDS             7852..9117
                     /locus_tag="ACZPDN_05385"
                     /inference="COORDINATES: protein motif:HMM:NF022641.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HIR1298087.1"
                     /translation="MANENEKNPSALEELRQLKRTDRIKSLDIHEPELKGFNAENIFH
                     IDEEPEKKTLFGKRSAKTHVKINENIFDDEVSESVEITPNTSIDEVEAPIEKHTVEPV
                     MGKRDEKVEVEEAKKVVVETIDADENFTTESIENELKAFNVNPVEEEKTSELVEEEST
                     PVLSEEDIYDDEYDEEAEEEDENLYEERKSFLYAEYPKIEEYLQRKSNEGYHFVRHEG
                     KKYYFRKGQPKTYYYSMNYFVDEPDADQWRQWEADGWKLVSKSDGKKKSDAGWFTFRN
                     EEEDGEYRKEIDNDSEKFRFFKKYSSSCRSTMFLVFICMACCVVTGLLQVYFQGYLAG
                     IALCAGLFLISFIVFCMYGRMLRKSKKRARELKSKLRIREIRANERSKDFYDTSESEE
                     ELDTDWNTLEQHTAKQKKKIFKRVDDDEE"
     gene            complement(9144..9899)
                     /locus_tag="ACZPDN_05390"
     CDS             complement(9144..9899)
                     /locus_tag="ACZPDN_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1298088.1"
                     /translation="MLKKERLVKIAQKVNQNGIITIQEIMDELGVSDMTARRDLDELE
                     KSGKLLRVHGGAQSLSFSMDHELSHIEKSSVQIEEKKRIAHKAATLISEGESIFLGPG
                     TTIQFLAEQLTGRNIRVITNSLAVFNILVNHAPTEVILTGGDYRDNTNTFVGPITNLV
                     LGKLKYNKAFISCNGIYNSSITTYSLEEGESQQIALDNSKNKYLLADNKKFNRADFYI
                     YYDLFNFDTLITDNTIDEEVLAHYKQYVQIEKV"
     gene            10139..10789
                     /locus_tag="ACZPDN_05395"
     CDS             10139..10789
                     /locus_tag="ACZPDN_05395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004802962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1298089.1"
                     /translation="MYKGVIFDFNGTLFFDNPKHILAWSKISQDIRHHGIDEEELHVH
                     INGVPNNKVVEYMFGGTCDPSEMEKYSQLKEEYYRQFCKEDQATFHLVKGAEEYFDYL
                     KESNIPFTIASASIKPNIDFFVESFHLDKWIEPSMIVYDDGSYENKVAMFIKAANNIG
                     VDIQDCLIIEDSASGISNAYKAGCHNIVVVDSANKKEEYEKLPGVTQVIDSFENFK"
     gene            11109..>11317
                     /locus_tag="ACZPDN_05400"
     CDS             11109..>11317
                     /locus_tag="ACZPDN_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1298090.1"
                     /translation="MNTKINQLLEYALKNGMIEEYDYDYSANLLIDLFGIYEFKREEV
                     EDCSIYEILDFMLDYAVEKGMIQDTV"
BASE COUNT         3824 a         1473 c         2300 g         3720 t
ORIGIN      
        1 acagtttttt tgtttcttgt tgacaaatta ttctttatag ggtataactt aagtagttag
       61 atgaaactaa ctatcactaa atgcaattat ggaatcgctc ttttcacttg tttaataatt
      121 tgatttcata agatgaggac attttaaaaa tgtccttttt tgtttacaaa ttaaaaaagt
      181 aagactatac taactgtgat taggggaaac taacaaaaaa gtggatagtt tttcctaacg
      241 aaaaaaactt gatgtttgat ttttcaagtg ttatcttttt tttaccgttg aaaaggagga
      301 tctatatcat gaaagatgga tggaaaaaca agacaatgtg gacttttatt ggaggtgcag
      361 ccactgcttt agctggttca aaatttttga agagtgatac agcacacgat ttagctgtaa
      421 aaggtttagc tggtggtatg aaatttaaat ctgatacaac ttgtaaatat gagacaatca
      481 aagaagatgc taaggatctt ttagaagaag caaaagttaa aaacgaaggc taattcaaga
      541 tgatttcatt tacaatcgcc cacgatatac ctggaagaat gcgtatacgt tatgggaaag
      601 atgtgttttc acccgttgaa ggtgaagttt taaaaagaga tcttagcaat tggaccatga
      661 ttcatagtgt agaagtgaat tcaattaccg gaagtgtttt aatgcagtat gatgcaactt
      721 gtaaagatac tttattgaag aaacttcata atttgaatct tggatattta aaagaagcgg
      781 atacttccga tattgtattt cgctcacaaa cgccagaagt aaaggcaatg aatgcagaat
      841 ataaacgtcg ttttttcaaa ataattggga aacgatattt tatacgttgg tttttaccaa
      901 caggaatcgg aaatgcaatt acgatttata aatcacttgg atttataaaa gaaggtttaa
      961 aatccttatc gcaaatgaaa gtaaatgttc cgctactgga tgcgacaagt ataggtgtga
     1021 gtcttttaca aaagaatttt aatattgccg gtaatattat gatgttgctc aatatttcag
     1081 atttattaga ggactataca cgtaagaaaa caaacttgga attatcgcaa agtttatcta
     1141 tacagtttga taaagtttgg atctatgccg atggagtgga acaagagatt cctatggcaa
     1201 aacttcaaaa aggggatatt gttgtttctc aaatgggagc gatgattcct atcgatggag
     1261 aagttgtgga tggagaagcc atggtcaatg aggctagctt tacgggtgaa cctttgtcta
     1321 gacgagtggt aaaggaggat acggtttttg ctggaacttt aattgaagaa ggaaaattat
     1381 ttattaaagt tcgtaattta caagatgaaa gtcgtatcag caatattgtg aaaatgattg
     1441 ataccaacga atctttaaag gcgagcattc aggcaaaagc cgaacatttg gcggattcca
     1501 ttgtgccatt tagttttttt ggattctttg gaatcttggc acttacacgt gatgtgacaa
     1561 gagctacttc tgtattaatg gttgattatt cgtgcgcaat acgattgtcc acttctattt
     1621 ctgttattag tgcgatgttg gaagcgagca atcggaatgt ccttgttaaa ggcggaaaat
     1681 atttagaagc tgtaaaagat gcggatgtta ttgtatttga taaaacaggt acattgacaa
     1741 atgccaaccc tcaagttcaa aaagtaactc cacttaatgg atatagtcga gatgaggttc
     1801 tacgtattgc ggcttgtcta gaagagcatt ttcctcatag tgtagccaat gcgattgtca
     1861 atcaagcaaa aaaagaaggc ttggttcatg aagaagaaca tgccaaagtg gaatatatcg
     1921 ttgcacatgg cattgcgaca accttacatg gcttacatac agtgattggc agcgatcact
     1981 ttatctttga agatgatggt gtcgaaaaga cacaggaatt agaagatatc attacttcat
     2041 tagagaaaga tggagccggt tctttgattt acttagccat tgataatcaa gtggctggta
     2101 tcatcagtat ctacgatcct ttaaaacaag aagcgaaaca agtgatccat gaattaaaga
     2161 atttaggtat tcaaaaagtt gtcatgttga ccggagattg caacaatgcg gccaaagctg
     2221 tagctaatga attggatctt gatgattatc gttcttcgat tttaccagaa ggtaaagcaa
     2281 gttatattca atctttaaaa gatgaaggcc atacagtaat catggttggt gatggtatta
     2341 atgatacgcc tgctttatct acagcgaatg tttcgatatc tatgcaggac agttcggatt
     2401 tagctcgaga acttgcggat gttacattag tttctagtaa cttaaatgaa ttggtaactc
     2461 taagaaaact atcggtttct ttgtttgatc gaattcacgc caattatcgt tttattgttt
     2521 cgtttaattc tagtttgatt gcacttggag ccttgggatt tatgagtcca aatacttcaa
     2581 gcttattaca taatggaagt acatttgcga ttgcggcaac aagtacgcga aaatatcttt
     2641 aggcattatc ggttgatgat gccttttcgt ctgtttataa tatagagatg gaggatttct
     2701 atggtacaaa aattaaatca ttctgaatta aaggatatta cagatattgt gtattataca
     2761 aatttaaaac aaaaaaattt agttttacga aatacattgg tgttgggtta taccatagcg
     2821 ggttcgcttt tatgttgggt tgtaggagct agcttaattc gcgtagcatc cggtgcatcg
     2881 gtattcttat ttctttgttc cttgatttgt ctttgtgtag taatttatat tcttatctgc
     2941 cgttttttgg agaagagaag atgtagaaag agggtacagg aaaaagaaac gggtgaattt
     3001 aatgttcact ttcgtaaaga ttatatgttg tatgatcaac agacattttc tttaaaacga
     3061 tttaagaaag tatatctgta tcatgattta tattattttg tggattcaga gaatgttgtg
     3121 attgtaaaaa aatgtcaaga agtggatcgt gcgattcata aaacacctta tttaaaagta
     3181 caaaaaaaag atgcgttatt ttgtttgttg taaacgcatc tttttatttt atactgcttt
     3241 tgatgtatat ccggcttttg ttacaatatc aatcaattca tcagttgtat gttcttgttt
     3301 agataatatg gtaacagtat tatcttccgt gtttactttg gcataaaagc cttcttggct
     3361 attgaacgca ttttcaatgg tatgaacaca attttgacag tgaatgtcat atacttttaa
     3421 aactactttg taagggtaat gctctttgtt tttatctttt atccttattt ttttaacagt
     3481 ttcacttgat ccacaacatc ctgaggtggc tctttttttc gtggaacgaa ttccaataca
     3541 gataataata attaaaatga cacaaataat aattgttggt aagttcataa tttgatgtcc
     3601 tcctttcaaa aaaaatacac ctattagtta gatatgtcaa actgagttaa tcttgtgcaa
     3661 ctttttgttg acattactga atggtgagag taaactaact gtgttagaaa tatctaactg
     3721 ggaggattcg atatgccatt atcttttgca gatttaaatt cagtcaatac aatcaaaaaa
     3781 attggtggtt ctaaagaaca acaacgacac ttagaaaact tgggctttgt gcctgggact
     3841 gtaattgtgg ttgtgagtaa aaccaatgga aatgtcattg tgaatgttaa agaggttcga
     3901 atagccatta gtgaagaact cgcaagaaag atcatggtgt agaaaggagt aattatgact
     3961 ttaaataaag tttcaattgg tcaaacagtt actgtaaaaa aattgtctgg tcaaggagca
     4021 acaaaacgaa gaattatgga tatgggaatt actaaaggag taagtgtaac tgttcaaaag
     4081 gtagcaccat taggagatcc tattgaatta acggttcgtg gatatcaatt gtcgcttcgt
     4141 aaagcagacg cagaaatgat tgaagtagag taaagctaaa aaataggagg gaattatgtc
     4201 aatacaagtc gcattagcag gtaatcctaa ctgtggtaaa actacattgt ttaatggttt
     4261 aacaggtgct acacaatatg taggtaactg gccaggggtt acagttgaga aaaaagaagg
     4321 aaagtataag gaagataaag atattaaaat tacggattta ccaggtattt attcattatc
     4381 accatacact ttggaagaag ttgtatctcg tgaattctta ttaaatggaa atgtcgatgt
     4441 tgttttaaac atcattgatg gttctaactt agaaagaaat ttatttttaa caacacaaat
     4501 tttggaattg ggtattccaa ccgttgttgc gatcaacatg ttggatgtca tcgaaaaaag
     4561 aaaagataca attgactata aaaagttatc cgaagaatta ggttgtcctg tattaccaat
     4621 ttccgcattg aaaaatacag gaatcaaaga gttgatggct caagtaaaaa aggcagcagg
     4681 tacaaagttt agtgctaaaa acatttatgc aggtaaagta ttaaatgcct tgaatacaat
     4741 tgaaacaagt ttaccttcaa gcattgaagc agatcgtcgt ttcttctatg ccgttaaatt
     4801 atttgaaaga gatgataaaa ttgaagcagc tatcaagtca aaggcagatg ctagtgtgat
     4861 tgaagcttgc gaaaagtcaa tggatgatga tagtgaatca attattacgg atgctcgtta
     4921 tacatatatt acaagtgtaa ttaaggattg ttacaaaaaa ggatcaaaag aagtattaac
     4981 aacttcagat aagattgatc gtattgtcac aaatcgtgta ttagcacttc caatctttgc
     5041 ccttgtaatg tggtttgtat attatatttc agtaactaca gtaggaacga ttgtaacaga
     5101 ttggacaaat gatgtattgt ttggtgaaat tattccacca gcagtggata gtttcttaga
     5161 tgcgattcaa tgtgcagatt ggttacatgg attaattgtg gatggtatta ttggtggtgt
     5221 aggagctgtc attggctttg taccacagat gttagtctta ttcttcttct tagctatctt
     5281 agaagattgt ggatatatgg cacgtgttgc ctttatcatg gaccgtattt tccgtaagtt
     5341 tggtttatct ggaaaatcat ttattcctat gttgattggt acaggttgtg gtgtgcctgc
     5401 cgttatggca agtcgtacca ttgaaagtga tcgtgatcgt aagatgacaa tcatgacaac
     5461 aacatttatt ccttgtggtg ctaagatgcc aatcattggt ttgattgccg gtgctatttt
     5521 ccatggtgct agctgggtag ctccaagtgc ttactttgta ggtatggcag ctgttattat
     5581 ttctggtatt atgttaaaga agactaaatt atttgccgga gatcctgcac cattcgttat
     5641 ggaaatgcca gcataccaca cacctcgtgc agttaatgta ttaagaagta tgtgggaacg
     5701 tggttcaagc tttattaaaa aggctggaac aattatctta ttatctacaa tcgtattgtg
     5761 gttcttacaa ggatttggat gggaaaaagg tgcgtttggc atggttgatg atattgatca
     5821 ttcaattcta tcttcaattg gacaaacatt cgcatggatc ttctcacctc ttggatgggg
     5881 cgattggaaa gctgcggttg ctagtgtaac tggtttaatt gcgaaggaaa atgttgtcgc
     5941 aacatttggt caattgtatg gttttgccga agttgctgaa gaaggaaatg aattctgggg
     6001 tcaattatct gcaagcttta cacctttagc tgcttattca tttatggtat ttaacttatt
     6061 gtgtgctcca tgttttgcag caatgggagc tatcaaacgt gaaatgaaca acacaaaatg
     6121 gttctggatt gccattggat atcaatgtgg atttgcctat gtatgttcat tgattgttta
     6181 tcaattagga agcttagtca atggtggatc atttggcttt ggtgctattg taggatttat
     6241 cttattgatt ggtttgatct atctattggt tcgtccatca aaagaaagtg atactgcaga
     6301 agttgaattt gcggtaaaat aatgtaaaag gagggatttc atgaatctat caacatttct
     6361 agtattatta gttgtatgtg gatgtgcagg tttagcttgt cgaagcattt ataaagataa
     6421 aaagagtgga aaatcaagtt gtggtggtag ctgtgggcat tgcggctctc attcgatgtg
     6481 tgaaaatccc aaagcatttt ttgatcaaat aaaaaaagaa caatttagta aataagagca
     6541 tcgaaaggtg ctctttcttt tggtataata ggtgcaagaa tcgaggaaaa taaaatgaat
     6601 ttaaaagaga aatatgcaga cttaattgtt cgttcaggat tgaatgttca aaaagaccaa
     6661 atagtagttg tacgtgccaa tgtagaaatg aatgaatttg ttcgtttgat tgtaaaatca
     6721 tgttttgagg ttggcgcaaa agatgtcatt gtacgttata gtgatgatga aatcaatcgt
     6781 atgagatatc tttcgggtaa tcgtatgcct tatgattatg aagctttgtt tcataatgaa
     6841 acggctgaaa aaggagcttg ttatttgagt ttagttggag aagatccaga tggaatgaaa
     6901 ggtgtagatc ctaaactaat gtcaaatttt agtaagacat taagagggat gactcagcca
     6961 tatcgcgata aattagatta tatgcatgca gcttggtgtg tggccagtgt tccaacaatg
     7021 gcttgggcca aaaaggtata tccagaatca gaagatgcat tatctgattt atggaatgcc
     7081 gtattaaaaa tgagtcgcgt taatgaaaag gatgcgaatg aaaactggaa tgagcatcgt
     7141 gcttcttttg ataagcgagt tcatattttg aatcatttgg atattgaaag tgttcattat
     7201 acaaattctt taggaacgga tttaatagtt gaacttccag aaggttacgt atttgcgggt
     7261 ggaggttcat tcttagataa tggcaattac tatttcccaa atattcctac agaagaaatt
     7321 ttctcggcac caaaaaagac gggtgtaaat ggtaaattat atagtgcttt accactagct
     7381 tataatgggg ctgttgtaaa agatttctgg tttgaattta aggatggtca agtcgttgat
     7441 tatgatgcca aagaaggaaa agaagttttg acaagtattc tagaaacgga tgagggatct
     7501 aaatatcttg gtgagattgc cttggttcct tatggatcac caattagtgc aatggatact
     7561 ttgttctatg aaacattgat tgatgaaaat gcttcttgtc attttgcctt gggtgctagc
     7621 tacaatgaat gtattgaaaa tggtttaaat atgagtgaag aagaattgtt agaacatggt
     7681 atgaatcaat cttttgcgca tgttgatttt atggtaggaa caagtgattt atcgattgag
     7741 gcgacattaa aaaaaggtga aaaaatcttt attttcaaag atggtaaata cacaagagaa
     7801 tttgacggat atacattaaa ttagtacaat agtagagttg gagggatatt tatggcaaat
     7861 gaaaatgaaa agaatcccag tgctttagaa gaacttagac agttaaaaag aacggatcgt
     7921 attaaatctt tagatataca tgaacctgaa ttgaaaggtt ttaatgcaga aaatattttc
     7981 catattgatg aagagccaga aaagaagaca ttatttggaa aaagaagtgc aaaaacacat
     8041 gtgaaaatca atgaaaatat atttgatgat gaagtgagtg aatccgttga gattacacca
     8101 aatacttcga ttgatgaagt agaagcgcca atcgaaaagc atacagtaga acctgtgatg
     8161 ggtaagcgtg atgaaaaagt ggaggttgaa gaagcaaaaa aagtggttgt tgaaactatt
     8221 gatgcagatg agaattttac cacggaatcg attgaaaatg aattaaaagc ttttaatgta
     8281 aatccagttg aagaagaaaa aacatctgaa cttgttgaag aagaatcaac tccagttttg
     8341 agtgaagaag atatttatga tgatgagtat gacgaggaag cggaagagga agatgaaaat
     8401 ctttatgaag aaagaaaaag cttcttatat gcggaatatc caaaaataga agaatatctt
     8461 caaagaaaat caaatgaagg atatcacttt gttcgtcatg aaggaaagaa atattatttt
     8521 agaaaaggtc aaccaaaaac atattattat tcaatgaact attttgtgga cgagcctgat
     8581 gcagatcaat ggcgtcagtg ggaagcggat ggttggaaat tggtttctaa atcagatggt
     8641 aaaaagaaaa gtgatgcggg ttggtttact tttagaaacg aagaagaaga tggagaatat
     8701 cgcaaagaaa ttgataatga ttctgaaaaa ttccgtttct ttaagaaata ttcaagctca
     8761 tgtcgttcaa caatgttctt agtatttatt tgtatggcat gttgcgttgt gacaggacta
     8821 ttacaagtgt atttccaagg ctatttagca ggaatcgcat tatgtgcagg tctattctta
     8881 atttcattta ttgtattctg tatgtatgga agaatgctta gaaaatcaaa gaagagagct
     8941 cgtgaattaa aatcaaaatt aagaattcga gaaattcgtg caaatgaaag aagtaaagac
     9001 ttctatgata cttctgaaag cgaagaagaa ttggatacgg attggaatac attggaacag
     9061 catacggcta aacaaaagaa aaaaatattt aaacgtgtag atgatgatga agaataaaga
     9121 ggcaaaataa atcgcctctt tttttaaact ttttcgattt gtacatattg cttgtagtgt
     9181 gctaatactt cttcatcgat ggtattgtct gtaatcaatg tatcaaagtt aaatagatca
     9241 taataaatat agaagtcagc acgattgaat tttttattat cagccaatag atatttattt
     9301 ttagaattat ctaatgcgat ctgttgtgat tctccttcct ctaacgaata ggttgtgatg
     9361 gatgagttgt agattccgtt gcagctaata aaggctttgt tgtattttaa tttacccaat
     9421 actaaatttg tgatgggacc tacaaacgta tttgtgttat ctctataatc accgccagta
     9481 aggataactt ctgtaggagc atgattcact aatatattaa atacggcaag actgtttgtg
     9541 atgacacgaa tgtttctgcc ggtcaattgt tcagctaaaa attgaatggt tgtaccaggc
     9601 cctaaaaata tggattcacc ttctgaaatg agagttgcag ctttatgggc aattctcttt
     9661 ttttcttcaa tttgaaccga tgatttttca atgtgactca attcgtgatc cattgaaaaa
     9721 gaaaggcttt gtgcacctcc atgaactctt aacaattttc cggatttttc taattcatcc
     9781 aaatctcttc gtgcagtcat atcggagacg cctaattcat ccatgatttc ttgaattgta
     9841 ataattccat tttgatttac tttttgtgct attttaacta aacgttcttt ttttaacata
     9901 ttgatatcct cttctatgtt aaaagatacc atttcaaata tatttagtca acacaaaaca
     9961 aacaaaaaca aacaaaatga agttatatat tacttaaatc taacataaat gtttgaaaaa
    10021 taataaatat atgtttgaaa atgttgactt tttagaagaa aatgcttaca ctgaagttat
    10081 atgaggttga ttacttttta atgcatgtaa atgattttaa ggttagggtg atttcgtaat
    10141 gtacaaaggc gtaatatttg attttaatgg aacattgttt tttgacaatc caaaacatat
    10201 tttggcatgg tcaaaaatat cacaagatat tcgtcatcat ggaattgatg aagaagaact
    10261 tcatgtacat atcaatggtg tacctaataa taaagttgtt gaatatatgt ttggtggaac
    10321 gtgtgatcca tccgaaatgg aaaaatattc gcagttaaaa gaagaatatt atcgccaatt
    10381 ttgtaaagaa gatcaggcga catttcattt agtaaaggga gctgaagaat attttgatta
    10441 tttaaaagaa tcaaatattc cattcacaat tgcatccgca tctatcaaac ctaatattga
    10501 tttctttgtc gaatcatttc atctagacaa atggattgag ccatctatga ttgtctatga
    10561 tgatggatcg tatgaaaata aagtcgcaat gtttataaag gctgccaata atattggtgt
    10621 ggatattcaa gattgtttaa ttattgaaga tagtgctagt ggaatttcaa atgcttataa
    10681 agcaggatgt cataatatag tcgttgtgga ttctgccaat aaaaaagaag aatacgaaaa
    10741 gcttcctgga gttacacaag tgattgattc ttttgagaat tttaaataag agccattcat
    10801 gccacaatgg ctctttgtat aaataaaaca taaaataaac aaaaacaaac aaataatcat
    10861 tattttaaac ctaaatctaa catctgtgtt taaattgttg tttgaatatg ttgacacaga
    10921 taaagaaaac gcttacacta aagttaaatg ggcaaatctt acatttataa ccgcatctgc
    10981 aaaatattga tgatgagccg tatataaata aatgaataat tgatcattat ttttgtgtgt
    11041 aatgtagttg gaaaaaggat ttgactgtga taaaaatgat tgattttgtt tatatataag
    11101 gagaacaaat gaacacaaaa attaaccaat tattagagta tgctttaaag aatggaatga
    11161 ttgaagagta tgattatgat tattctgcaa atttattgat tgacctattt ggcatctatg
    11221 aatttaaaag agaagaagta gaagactgtt ctatctatga gattttggat ttcatgttag
    11281 attatgcagt tgaaaaaggt atgattcaag atacagt
//
LOCUS       DBMDWL010000058        11259 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1982_length_11259_cov_2.873723, whole genome shotgun sequence.
ACCESSION   DBMDWL010000058 DBMDWL010000000
VERSION     DBMDWL010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1982_length_11259_cov_2.873723"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(211..717)
                     /locus_tag="ACZPDN_05405"
     CDS             complement(211..717)
                     /locus_tag="ACZPDN_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HIR1298091.1"
                     /translation="MEKEARLQAIYDTISKSEGPVSGTALSKKFNVSRQVIVQDIALL
                     KARNVHIYATHKGYVCSPQRVRRVFSCFHLNDRMEEEMNAIVDCGGCIENVFVKHDVY
                     GMLEAKMNISSRKQVKEFIDSISQNQAKPLTDLTSGYHCHTVSAQSKEDLDSIEESLR
                     KLHILQEA"
     gene            complement(718..1569)
                     /gene="nadC"
                     /locus_tag="ACZPDN_05410"
     CDS             complement(718..1569)
                     /gene="nadC"
                     /locus_tag="ACZPDN_05410"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR1298092.1"
                     /translation="MDKNTMRLNVDHLLIQALQEDITSEDVSTNAVMPSYQYGQVQLI
                     CKEDGIIAGLEVFERVFYLLDESMEVKFYVKDGDAVKNGQLLAEVSGDVRVLLSGERT
                     ALNYLQRMSGIATYTNRVVSYLKGSKTKLLDTRKTTPNMRVFEKYAVRVGGGNNHRYN
                     LSDGVMLKDNHIAAAGSITNAIKMAKNYASFVRKIEVEVETLEQVKEAVEAGADIIML
                     DNMSVDNMKKAIEIIDGRAETECSGNVTKENIEKLTALGVDYISSGALTHSAPILDLS
                     LKNLQVK"
     gene            complement(1562..2854)
                     /locus_tag="ACZPDN_05415"
     CDS             complement(1562..2854)
                     /locus_tag="ACZPDN_05415"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR1298093.1"
                     /translation="MEKLEADIVIVGTGASGLFAALHVPSDKKVIMLTKSDAENSDSF
                     LAQGGICVLRDKNDFDSFMEDTMKAGHYENRKESVEIMINSSRDIINELIGYGVDFAT
                     KDGKLDYTREGAHSKPRILFHEDITGKEITSTLLDEVRKLENVTIMEYVTMTDIIEKD
                     NVCYGVLAKDANNNEMCIEAGYTIFACGGIGGLYAHSTNYPHLTGDAIAIAIQHGIKL
                     ENVDYVQIHPTTLYTKKKGRAFLISESVRGEGAKLYGKDRKRFANEVLPRDIMTQKIR
                     EQMKKDDMPYVWMDMSVLGEEVIKNHFPHIYEKCLEEGYDCTKEWIPVTPAQHYFMGG
                     IHVDKDSRTSMSHLYAVGETSCNGVHGKNRLASNSLLESLVWGKRAVVDLLKKEPAYT
                     SDFDCLAKVAKSHQLSNQEKMYHDLVLEEIERERQARG"
     gene            complement(2856..3761)
                     /gene="nadA"
                     /locus_tag="ACZPDN_05420"
     CDS             complement(2856..3761)
                     /gene="nadA"
                     /locus_tag="ACZPDN_05420"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR1298094.1"
                     /translation="MSLKEDVLKLKKEKNAIILAHYYVDGAVQDVADFVGDSYALAKK
                     AKNTDADIIVFAGVKFMGESAKMLNPEKKVLMPDLNADCPMAHMASKAVIKKMKEEYE
                     DLAVVCYINSTAELKTMADICVTSSNAVKIVRSLPNKNIFFIPDGNLGAYVKKQVPEK
                     NIILNDGYCITHKRVTLKDVEKAREEHPNVPIIVHPECVNEVVEATDFAGSTSELIQY
                     TVDNPNKEFVIGTELGVLHEMQLKSSDKTFYPLTDKLICMNMKKVSLEKVYDCLLNET
                     NEVFVDEEVKAMAEKSLDRMLELAK"
     gene            complement(3923..4432)
                     /locus_tag="ACZPDN_05425"
     CDS             complement(3923..4432)
                     /locus_tag="ACZPDN_05425"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR1298095.1"
                     /translation="MLSKKVADLLNNQVNKELYSAYLYLDFENYYHDKSLEGFANWYH
                     VQVQEEIAHAQLMMQYLQDNDYSVVLEAVNKPNIPLNELSDPLKAGLKHEQYVTELIH
                     TCYAAAYEEKDFRTMQFLDWFVKEQGEEEKNASDLISRFELFGHDAKGLYDLDAEYKT
                     RTYIAPSIL"
     gene            complement(4497..4958)
                     /gene="bilS"
                     /locus_tag="ACZPDN_05430"
     CDS             complement(4497..4958)
                     /gene="bilS"
                     /locus_tag="ACZPDN_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024055.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HIR1298096.1"
                     /translation="MIGIVYSSKTGNTQSLANSLNFMESKYCVSVIDAKDLEPVDLLC
                     IGFWCDKGDCDDSMKQYLSTLRNQNVFLFGTAGFGKSKEYFEMILKNVQSHLDASNCV
                     KGTWMCQGKMPLAIRAKFESNPEMLKNFDEALKHPNDKDVDSLMKSVNEAL"
     gene            complement(4972..5220)
                     /locus_tag="ACZPDN_05435"
     CDS             complement(4972..5220)
                     /locus_tag="ACZPDN_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298097.1"
                     /translation="MEIIEAIEKLKESHALVCLENRALIKMEKDKVYIWHNQWHSKLS
                     RNDFISLFQKNTFEVYENDHGIDMEKDEEYYAWRNRYL"
     gene            complement(5284..5826)
                     /gene="rbr"
                     /locus_tag="ACZPDN_05440"
     CDS             complement(5284..5826)
                     /gene="rbr"
                     /locus_tag="ACZPDN_05440"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR1298098.1"
                     /translation="MELKGSKTEKNLMTAFAGESQACVKYQYYASQAKKDGYVKIQNI
                     FAETSNNEKEHAKLWFKALHGGKVPTTTENLKDAAAGENYEWTDMYATMAKEAREEGF
                     DDIAALFEGVARIEKHHEARYKAMLKDVEEDAMFKKDTSTVWICLNCGHIHYGETAPL
                     VCPVCNHPQAYFQELKDHYE"
     gene            complement(5924..6313)
                     /locus_tag="ACZPDN_05445"
     CDS             complement(5924..6313)
                     /locus_tag="ACZPDN_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Inorganic pyrophosphatase"
                     /protein_id="HIR1298099.1"
                     /translation="MQQLENNAFFWQKLDTLLLSSSCTIDRPKGSAHYKFTNLIYPVD
                     YGFLSDTFGSDQKPIDVFKGSIATNNVDAIAITADILKKDCIVKLLVGCTEEETMRIL
                     EFLDQTEFQKAILVRRGNETPSWSNND"
     gene            complement(6391..7374)
                     /locus_tag="ACZPDN_05450"
     CDS             complement(6391..7374)
                     /locus_tag="ACZPDN_05450"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR1298100.1"
                     /translation="MNKKNITIIATGGTIAGRGSVGKSANYQAGTFDVEEIVQSIPQI
                     RDLANLNTIQLCNVDSNDMGLDKWVELRNICHELEKNRYVDGIVITHGTDTLEETAFF
                     LNLTLACHKPVVLTGSMRPATATSADGPMNLYQAVALACHMEAWQAGVLAVISDTIYS
                     GRDLQKTNSYKTDAFKMGEFGSLGYMRDDHVYLLNSPFKKHTFQTIFSKMEFEDLPKV
                     EIFYVHTDSDPELLEYMLNKYDGLVLAGTGSGNYSTKIKEVLETYAGNCTIVRASRVL
                     EGAVFDSDVFDSKKVTIPAYKFSAQKARILLMLALNCTKNHETIKSFFQEY"
     gene            complement(7367..7876)
                     /locus_tag="ACZPDN_05455"
     CDS             complement(7367..7876)
                     /locus_tag="ACZPDN_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298101.1"
                     /translation="MKKIITLLCTFVLCIGLVGCESKTPFEIKEISATKLQKKLDNKD
                     SFVLIVERENCPYCESLQEYIDKTKDEHPNLVLYKIDSTDYGFSKISQDSKQLQSSTK
                     DGKILLDMAPYFLYTPTLYVIENGKAKHAGIGYNESDHSISLWDVDSTIDFDLADTQE
                     FWEFLETYE"
     gene            complement(7885..9018)
                     /locus_tag="ACZPDN_05460"
     CDS             complement(7885..9018)
                     /locus_tag="ACZPDN_05460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR1298102.1"
                     /translation="MKIIVASDSFKGCMSSKEVEESIKKGILKANDAHEVLTFPMADG
                     GEGTAMVFCDIIKGKMIDVLTTDAYGKNVFTKYCISQDGNLAVMDVASCIGLNMTPKE
                     KRNPMIASSKGVGIMMKDALSRGCKKIIIGLGGSATNDGGMGVLSEFGVRFYNSKREL
                     LVPSVYALSQIAFVDKRYARLPKDVEIICACDVKNHLLGKNGATYVFGKQKGIYLNQM
                     SEVERGMAHYCMKLKQTFHVNVNEFEGSGAAGGIGSVLLGVMQAKRVSGIDLVVEYSG
                     LKEHLQNADLVITGEGQTDAQTLYGKLPLGIAHLARSYSVPCVCLSGALGLGYQSLYD
                     EGMIGIFSSADRAMSFQMALQTGSEKLEALAFSLTKFMDGIRK"
     gene            9192..10385
                     /gene="coaBC"
                     /locus_tag="ACZPDN_05465"
     CDS             9192..10385
                     /gene="coaBC"
                     /locus_tag="ACZPDN_05465"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1298103.1"
                     /translation="MKKHVVIGVSGGIAAYKACDLVSKLSKKDYEIKVVMTEHAQEFV
                     KPINFESLSKHKCEVSLFDETNEDPIAHITLAKWADIMVLVPATANIIAKVVHGIADD
                     IVSTTFLACHTKKLICPAMNVHMYENEVTQKNIKLAKELGYKFVEPVVGHLACNDTGK
                     GKLADVQDIVAAIDHEFELEQTLKGKNVLVSAGPTQEALDPVRFLSNHSSGKQGYAIA
                     KAAKALGANVTLVAGPTALEDLKDVNMVHVTNAQDMFDAITSRLDDQNYIIMAAAVAD
                     YRPEMVADQKIKKSDEEVTFHFVKNPDILAYIGQHKKENQVICGFAMETQNLEENARK
                     KLESKNCDMLIANNLFTRGAGFQTDTNVVTLLRKNETKHLPKCSKEELGYKILETMKE
                     IEMEK"
     gene            10387..11154
                     /locus_tag="ACZPDN_05470"
     CDS             10387..11154
                     /locus_tag="ACZPDN_05470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1298104.1"
                     /translation="MLVTVDIGNTNITIGIFDKDDLIGTYRLTTKVRRTSDEYGFMLK
                     NFLDRSNVHEKQIDAVIVSSVVPKMMHSFTNGIKKFVGIEPLIVGPGVKSGISVQLEN
                     PRNVGADRIADCAGAFNEYGGPILVADFGTATTYDYVDENGCFKAGAIGVGIETGANA
                     LWGQTAQLPEIEIKRPKTILARNTQTEMQAGVFYQFLGGVEYTIAQFKKEVGKDMKVI
                     ATGGLGRIVCNYTKSIDVYDPNLIFKGLKVIYEKNKD"
BASE COUNT         3621 a         2217 c         1578 g         3843 t
ORIGIN      
        1 atgcattaat tgcagaataa tcataatata aagaatacat aattaaaggc atagctacaa
       61 agctaaacaa aaaagagagt gttagctgca taataatata tagccataat gagcctccaa
      121 ctttactcaa ttctttatcg ggcataaatt taggtggttg ttccataaga atcactactt
      181 tctaaattta ttctaacaca aaagaggccc ttaagcctct tgtaaaatat gaagttttct
      241 taatgattcc tcaattgaat ctaaatcttc ttttgattgt gcactcaccg tatgacaatg
      301 atatccactc gttagatccg ttaaaggttt tgcctgattc tgtgaaatag aatcaatgaa
      361 ttccttgact tgttttctag aactaatgtt cattttagct tctaacatac catatacatc
      421 atgtttgaca aatacatttt caatacagcc accacaatct acgatcgcat tcatttcttc
      481 ttccatacga tcattcaaat gaaaacaaga aaatactctt cgaactcttt gaggtgaaca
      541 tacatatcct ttgtgtgtcg cataaatatg aacatttctt gcctttaata aggcaatatc
      601 ttgcacaata acttgtcttg aaacattaaa ctttttagaa agcgctgttc cagaaaccgg
      661 tccttcactt tttgaaatcg tgtcataaat ggcttgaagt ctagcttcct tttccattta
      721 tttcacctgc agatttttta aagacaaatc cagaattggt gctgaatgcg ttagggcacc
      781 acttgaaata tagtccacac ctaatgctgt taatttttca atattttctt ttgtgacatt
      841 tccagaacat tctgtttcgg ctcttccatc aatgatttcg attgcctttt tcatattatc
      901 aacactcatg ttatctaaca taataatatc agcacctgct tcaacagctt cttttacttg
      961 ttctaaagtt tcaacttcta cttcaatctt acgaacaaaa gaagcataat tctttgccat
     1021 cttaatcgca tttgtgatag acccagccgc tgcgatatga ttatccttca acataacacc
     1081 atctgataaa ttgtatctat ggttatttcc accgccaacg cgcactgcat atttttcaaa
     1141 tacacgcata tttggtgttg tttttcttgt atccaataat tttgtctttg aaccctttaa
     1201 gtaagatacg acacgatttg tataagtggc aattccactc attctttgaa gataattcaa
     1261 agccgtcctt tcaccagata aaagaacacg aacatctcca gaaacttcgg ctaataattg
     1321 tccgtttttt acggcatccc catcttttac ataaaatttt acttccatgc tttcatctaa
     1381 caaataaaag actctttcaa acacttcaag acctgcaata atcccatctt ctttacagat
     1441 caattgtacc tgcccatact gataggaagg cattactgca tttgttgaaa catcttcact
     1501 tgttatatct tcctgcaaag cttgaatcaa taagtgatcc acatttaaac gcattgtatt
     1561 cttatccacg agcttgtctc tctctttcta tttcttctag taccaaatca tgatacattt
     1621 tttcttgatt tgacaactga tgtgatttag ccacttttgc caaacaatca aaatcacttg
     1681 tataagccgg ttcctttttc aataaatcaa caacggctct ttttccccaa accaaagatt
     1741 ccaacaagga attactagct aaacggtttt ttccatgaac accattacag ctggtttctc
     1801 caactgcata taaatgtgac atagaagtac gagaatcttt atctacatga attccaccca
     1861 taaaatagtg ctgagctggt gtaactggaa tccattcttt tgtacagtca tatccttctt
     1921 ctaaacactt ttcataaatg tgaggaaaat gatttttgat gacttcttca cctaaaacac
     1981 tcatatccat ccaaacataa ggcatatcat ccttcttcat ttgttcgcga atcttttgtg
     2041 tcataatatc acgtggcaac acttcattcg caaaacgttt tctgtctttt ccataaagct
     2101 tagccccttc accacgaacc gattcactaa taaggaaggc tcttcctttt ttctttgtat
     2161 ataaagttgt tgggtgaatc tgcacataat ccacattttc taatttgata ccatgctgga
     2221 ttgcaatcgc aatggcatct cctgttaagt gtggatagtt tgttgaatga gcatacaatc
     2281 ctccaattcc tccacacgca aagattgtat aacctgcctc aatgcacatt tcattgttgt
     2341 tggcatcttt cgccaaaaca ccataacata cattatcttt ttcaataata tctgtcattg
     2401 tgacatattc cataatcgtt acattctcta acttacgaac ctcatctaat aaagtggatg
     2461 taatttcctt tcctgtaata tcttcatgga ataaaatacg tggtttagag tgtgctcctt
     2521 cacgcgtata atctaatttg ccatcttttg tcgcaaaatc aacaccatat ccaatcaact
     2581 cattaataat gtcacgactt gaattgatca tgatttcaac agattcttta cgattttcat
     2641 aatgccctgc cttcatggta tcttccataa aagaatcaaa atcattcttg tctcttaaca
     2701 cacaaattcc gccttgtgct aaaaatgaat cactattttc ggcatctgat tttgtcaaca
     2761 taatgacctt tttatcacta ggaacatgta aagctgcaaa caatccactt gcacctgttc
     2821 caacaataac tatatctgct tctaactttt ccatactatt ttgccaactc caacattcta
     2881 tctaatgatt tttcggccat agccttcact tcttcatcta caaacacttc gtttgtttca
     2941 ttcaacaaac aatcataaac cttttctaaa gataccttct tcatattcat acaaattaat
     3001 ttgtctgtta aaggataaaa agttttatct gatgatttca actgcatttc atgaagcaca
     3061 ccaagttcag taccaatcac aaattctttg tttggattgt ctactgtata ttgaatcaat
     3121 tctgaagtgc ttcccgcaaa atcagttgct tcaacaactt cattgacaca ctcaggatgc
     3181 acaataattg gaacgtttgg atgctcttca cgcgcttttt caacatcttt taatgtcaca
     3241 cgcttatgtg taatacaata tccatcattt aagataatat tcttttcagg tacttgtttc
     3301 tttacataag cacctaaatt tccatctgga ataaagaaaa tatttttatt tggaagtgaa
     3361 cgaacaatct tcactgcgtt tgaacttgtt acacaaatat cagccattgt ttttaattca
     3421 gctgttgagt tgatataaca tacaaccgct aaatcttcat attcttcttt catcttttta
     3481 atgacagcct tacttgccat atgtgccatt ggacaatctg catttaaatc tggcattaat
     3541 actttctttt ctggatttaa cattttggca ctttcaccca taaatttaac acccgcaaat
     3601 acaataatat ctgcatctgt attcttagct ttttttgcca aggcataaga atctccgaca
     3661 aaatccgcta catcttgtac agctccatct acataataat gcgctaaaat aatcgcattt
     3721 ttttcctttt ttaatttcaa tacatcttcc tttaaagaca tggaattttt cctcccttaa
     3781 aaaattttac cgttttgaat tatacaccca ggctttacat ctgacaagac agttgtaaag
     3841 tcaatattat ataagttaca tgtcactatg aaataagcaa aaaaaaagcg atgaatcaaa
     3901 aatcatcgct ttagtttttt gtctataaaa tgcttggtgc aatataagta cgagttttgt
     3961 actctgcatc taaatcatac aagcccttgg catcgtgtcc aaacaattca aatcgagaaa
     4021 ttaaatccga agcatttttt tcttcttctc cttgttcttt gacaaaccaa tccaagaact
     4081 gcatagttct aaaatctttt tcttcataag ctgctgcgta acaagtatga atcaattcag
     4141 ttacgtattg ttcatgtttt aagcctgcct ttaacggatc acttagttca tttaaaggaa
     4201 tatttggctt attaacagct tccaaaacaa cactataatc attgtcttga agatattgca
     4261 tcatcaattg agcatgtgca atctcttctt gtacttgtac atgataccaa tttgcgaaac
     4321 cctctaaact tttatcatga taataatttt cgaaatccaa atacaaataa gcagaatata
     4381 attctttatt aacttgatta ttcaacaagt ccgctacttt cttactcaac atagcacatt
     4441 ccttctttct ggctacatca tactccaaac aacaagaaaa gcgtagtgat atgctctcac
     4501 aacgcttcat ttacagattt cattaaacta tcaacatcct tatcatttgg atgttttaaa
     4561 gcctcatcaa aattcttcaa catttcggga ttcgattcaa actttgcgcg aattgccaaa
     4621 ggcatcttac cttggcacat ccatgttcct ttaacacagt tcgatgcatc taaatgactt
     4681 tgaacatttt ttaaaatcat ctcaaaatat tctttagatt taccaaagcc agctgttcca
     4741 aacaagaaaa cattttgatt tcgtaatgtg gaaagatatt gcttcattga atcatcacaa
     4801 tctcccttat cacaccagaa tccaatgcac aataaatcta caggttctaa atcttttgcg
     4861 tctattacag atacacaata ttttgactcc ataaaattca aactgtttgc caatgattgt
     4921 gtgttccctg tttttgaact atatacaatt cctatcataa aatatatcct ttcataagta
     4981 gcgatttcgc catgcataat actcttcatc tttttccata tcaataccat gatcattctc
     5041 ataaacttca aacgtattct tttgaaatag tgaaataaaa tcatttcttg aaagtttcga
     5101 atgccattga ttatgccata tgtatacttt atctttttcc attttgatca acgcacgatt
     5161 ctctaaacat acaagtgcat gactctcttt taatttttca attgcctcta ttatttccat
     5221 aaaattattt tagcacaact ttctaggata agaaaaggta gccgaagcta ccatgagata
     5281 ttattattcg taatgatctt ttaattcttg gaaataagct tgtggatgat tacatactgg
     5341 acataccaat ggagctgttt caccataatg aatgtgtcca cagtttaggc aaatccatac
     5401 tgtagaagta tcctttttaa acattgcatc ttcttctaca tctttcaaca tagctttata
     5461 acgagcttca tgatgttttt caatacgagc tacaccttca aataatgccg caatatcatc
     5521 aaatccttct tcacgagctt ctttagccat tgttgcatac atatctgtcc actcatagtt
     5581 ttcacctgca gctgcatcct ttaagttctc tgttgttgtt ggaacttttc caccatgcaa
     5641 agccttgaac cataattttg catgttcttt ttcattattt gatgtttcag caaaaatatt
     5701 ttgaatcttt acatatccat ccttcttagc ttgtgaagca taatattgat acttaacaca
     5761 tgcctgagat tctcctgcaa aagctgtcat taaatttttt tctgttttag atccttttaa
     5821 ttccataata aagacctcct cttctagctt tattttaaat caactcaaca tgtttttcca
     5881 tttaaatgca taagaaaaag acaaatccta agacttgtct ttcctaatcg ttattcgacc
     5941 atgatggtgt ttcgttgcct ctacggacta aaattgcttt ttggaattct gtttgatcta
     6001 agaattctag aatacgcatt gtttcttcct ctgtacaacc cactaataat ttaacaatac
     6061 aatctttttt cagaatatct gccgtaatcg caatcgcatc cacattattt gttgcgatag
     6121 aacctttgaa aacatcaatt ggtttttgat ctgagccaaa tgtatctgaa agaaaaccgt
     6181 agtctacagg ataaatcaaa ttagtgaatt tataatgagc acttcctttt ggacggtcaa
     6241 ttgtacaact gcttgataat agaagcgtat ctaacttttg ccaaaaaaat gcgttatttt
     6301 ctaattgctg cataataacc tcctgtcttg taatagtcta taaattatta tagcatttac
     6361 aaaattattt acaatacaag aaaagttttt tcaatattct tggaagaacg atttaattgt
     6421 ttcatggttt ttagtacaat ttagcgcaag catcaataaa atcctagctt tctgtgctga
     6481 aaacttataa gcaggaatgg ttactttctt tgaatcaaac acatctgaat caaaaacagc
     6541 gccttctaat acacggcttg cacgaacaat tgtacaattt cctgcatagg tttctaaaac
     6601 ctcttttatc tttgtagaat aatttcctga acctgtacca gctaaaacta aaccatcata
     6661 cttatttaac atgtattcta ataattctgg atccgagtct gtatgtacat aaaaaatttc
     6721 tactttaggc aaatcttcaa attccatttt tgaaaaaatc gtttgaaatg tatgcttttt
     6781 aaatggtgaa tttaataggt atacatgatc atcacgcata tatcctagcg aaccaaattc
     6841 ccccatttta aatgcatctg ttttataact atttgtcttt tgtaagtctc ttcctgaata
     6901 gatcgtatct gaaataacag ccaaaacacc agcttgccaa gcttccatat gacatgccaa
     6961 agctacggct tgatataaat tcattggacc atctgcactt gttgcagttg caggacgcat
     7021 tgaaccagtt aagacaactg gtttatgaca tgctaaagta agatttaaaa agaaggccgt
     7081 ttcttctaga gtatccgttc catgtgtaat gacaattcca tctacataac gatttttttc
     7141 taattcatga caaatgtttc ttaattcaac ccatttatct agccccatat cattcgagtc
     7201 tacattacat aattgaatcg tgtttaaatt tgccaaatca cgaatttgtg gaatactttg
     7261 tacgatttct tccacatcaa aagtgcctgc ttgataattt gcacttttac caacgctgcc
     7321 acgtccagca atcgttccac ctgtcgcaat aatcgtaata tttttcttat tcatatgttt
     7381 ctaaaaactc ccaaaattct tgtgtatctg ccaaatcgaa atcaatagtt gaatccacat
     7441 cccataaact aattgaatga tcactttcgt tatatccaat tccagcatgt tttgccttac
     7501 cattttcaat cacgtaaagt gttggggtat atagaaaata tggagccata tccaaaagaa
     7561 tctttccatc ttttgtagaa ctttgtagct gcttagaatc ctgactaatt ttactaaagc
     7621 catagtctgt tgaatcaatc ttatataaga ctagatttgg atgttcgtct tttgttttgt
     7681 cgatatattc ttgtaaagac tcacaataag gacaattctc tctttcaaca attaacacaa
     7741 atgaatcttt attatctaat ttcttttgta gttttgttgc actaatttct ttaatttcaa
     7801 aaggcgtttt agattcacac ccaacaagtc ctatacaaag cacaaatgta catagtaatg
     7861 ttattatctt tttcatctaa aaacctactt tctaatacca tccataaatt tggttaaact
     7921 aaaggccaat gcctctaatt tctcacttcc cgtttgtaga gccatttgaa aggacatggc
     7981 acgatcagca cttgaaaaaa ttccaatcat accttcatca tatagggatt gatagcctaa
     8041 gcctaaagcc ccacttaaac aaacacatgg aacactataa gatcttgcca aatgtgcaat
     8101 acctaacggc agctttccat ataatgtctg cgcatcagtt tgtccttctc cggtaatgac
     8161 taaatccgca ttttgaagat gttcttttaa gcctgaatat tcaacaacta aatcgatacc
     8221 tgaaactctt tttgcctgca taacccctaa taatacactg cctatacctc cggctgcgcc
     8281 tgagccttca aattcattca catttacgtg aaatgtttgt ttcaatttca tacaatagtg
     8341 agccatacct ctttcaactt cagacatctg gtttaaatag ataccttttt gtttaccaaa
     8401 aacataagta gctccatttt tacctaacaa atgattcttc acatcacaag cacaaataat
     8461 ttctacatct tttggaagtc ttgcataacg cttatcaaca aaggcaattt ggctaagagc
     8521 atatacacta ggtacaagta attctctttt tgaattataa aagcgaactc cgaattcact
     8581 taaaactccc atgccaccat catttgttgc actaccacct aaaccaataa taattttttt
     8641 acatcctcta gatagtgcat ccttcatcat gatacccaca cccttacttg atgcgatcat
     8701 cggatttctt ttctcttttg gtgtcatatt caatcctatg caactagcta cgtccattac
     8761 agccaaattt ccgtcttgag aaatacaata tttcgtaaat acattcttac cataggcatc
     8821 cgtagttaat acatcaatca tctttccttt gataatatcg caaaatacca tggcggtacc
     8881 ttctccacca tcagccatag gaaaggttaa aacttcatgt gcatcatttg cctttaatat
     8941 tccttttttt atactttctt caacttcttt tgaggacata catcccttaa aagagtcact
     9001 cgcaacaatt attttcatgc gttcatttta ccatatgcca agaaaaataa acactttttt
     9061 aaaatttagg ttttcgaagt taaaatatcc ttagtgaatg ctttattgtt gcttgaaaac
     9121 gatggattca tatgggataa tgttttagtg aaaaatcaat acgtgatttt attcacaaag
     9181 gagaatcaat catgaaaaaa catgttgtta taggagtgtc tggtggtatt gcagcctaca
     9241 aagcttgtga tcttgtcagc aaattatcca aaaaagatta cgaaatcaaa gtggtcatga
     9301 cagaacatgc acaagaattt gtcaaaccaa tcaactttga atcattaagt aaacataaat
     9361 gtgaagtttc tttgtttgat gaaaccaatg aagatccaat tgcccacatt actttagcta
     9421 aatgggctga tatcatggta ttagttcctg cgacggccaa tattattgcc aaagtcgttc
     9481 atggaattgc cgatgatatt gtttcaacaa cttttctagc ttgtcataca aaaaaattaa
     9541 tttgcccagc aatgaatgtt catatgtatg aaaatgaggt cacacaaaaa aatatcaaac
     9601 tagccaaaga acttggctat aagtttgtag aacctgttgt aggtcatttg gcatgtaatg
     9661 atactggaaa aggtaagctt gctgatgtgc aagatattgt ggctgccatt gatcatgaat
     9721 ttgaattgga acaaacgtta aaaggaaaaa acgttttagt ttctgcaggt cctacacaag
     9781 aagctttaga tcctgttcgt tttttaagca atcattcaag tggtaaacaa ggatatgcga
     9841 tcgcaaaagc tgccaaagca ctaggtgcta atgtaacatt agttgcaggt cctacagctt
     9901 tagaagatct taaggatgta aatatggtac acgtgacaaa tgctcaagat atgtttgatg
     9961 caattacatc aagactcgat gatcaaaact acattattat ggctgctgct gttgcggatt
    10021 acaggcccga aatggttgcg gatcaaaaaa tcaaaaagtc agatgaagag gttacttttc
    10081 attttgtaaa aaatccagat attttggcat atattggaca gcacaagaaa gagaatcaag
    10141 tcatttgtgg ttttgcgatg gaaacacaaa atttagagga aaatgcacgt aagaaactag
    10201 aatctaaaaa ttgtgatatg ttgattgcga acaatctatt tactcgtggt gctggttttc
    10261 agacagatac aaatgttgta actttactta gaaaaaatga aacaaaacat ttacctaaat
    10321 gttcaaaaga agaattagga tataaaattc tagaaacaat gaaagaaatt gagatggaga
    10381 aataatatgc ttgtaacagt agacattgga aatacaaaca taactattgg tatttttgat
    10441 aaggatgatt taattggtac atatcgatta acaacaaaag taagaagaac gagtgatgaa
    10501 tatggtttta tgttaaaaaa ctttttggat cgttctaatg tacatgaaaa acaaattgat
    10561 gcagttattg tctcaagtgt tgttccaaag atgatgcact ctttcacaaa tggaatcaaa
    10621 aaattcgtag gtatagaacc ccttattgta ggacctggag ttaaaagtgg aataagtgtt
    10681 caattagaaa atccacgaaa tgttggtgca gaccgtatcg cagattgcgc tggagcattc
    10741 aatgaatatg gaggacctat tctagttgcc gattttggaa cagctactac ttatgattat
    10801 gtagatgaaa atggttgttt taaagcaggg gccattggtg ttggtattga aacaggtgcc
    10861 aatgccttat ggggacaaac tgcacaactt cctgaaattg agatcaaacg tcctaaaact
    10921 atcttggcaa gaaatacaca aacagaaatg caggctggtg tattttatca attcttaggt
    10981 ggagttgaat atacaattgc tcaatttaaa aaagaagttg gcaaagacat gaaggttatt
    11041 gcgacaggtg ggttaggaag aattgtttgt aattacacaa aatcaataga tgtgtatgat
    11101 ccaaacttaa ttttcaaagg cttaaaggta atatacgaaa agaacaaaga ctaattcaat
    11161 taaaatggac cctgtaaaac ggacacaata aaaaaagtac attagtacct agtcctgttt
    11221 tacagggctt ttattttgtg tattatgata attagagaa
//
LOCUS       DBMDWL010000059        11244 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1990_length_11244_cov_3.791618, whole genome shotgun sequence.
ACCESSION   DBMDWL010000059 DBMDWL010000000
VERSION     DBMDWL010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1990_length_11244_cov_3.791618"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(198..845)
                     /locus_tag="ACZPDN_05475"
     CDS             complement(198..845)
                     /locus_tag="ACZPDN_05475"
                     /inference="COORDINATES: protein motif:HMM:NF040019.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1298105.1"
                     /translation="MDKKRLRILVYVVGMFILALGLTLNTKANLGVSPIISVPYSISQ
                     ITGLNFGDLTFVVYAMFVVVQIIIHIRLKNHKRIASDILQLPLSLIFTRLLNIFTVYI
                     PTSQNLGIRFIILTFAIICTGIGAAMSLSMQIVPNPGDGIVQALAERFDKSVGITKNW
                     FDCLNLSITLCISLFIAHQIVGVGIGTVIAVVGVGRVIALFHHIFESKIEYLIFD"
     gene            complement(981..2288)
                     /locus_tag="ACZPDN_05480"
     CDS             complement(981..2288)
                     /locus_tag="ACZPDN_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1298106.1"
                     /translation="MTKITRDQRNFKFETLQLHVGQEQADPTTDARAVPIYQTSSYVF
                     RNCDHAAARFGLSDAGNIYGRLTNPTEDVFEKRIAALEGGVAALAVASGAAAVTYTIL
                     NLAQNGDNIVSAKNIYGGSFNLFEHTLPQYGIQTTFVDIFNEKEVENAINEKTKALYI
                     ETLGNPNSDVVDIESVARIAHRHKIPLVVDNTFATPYLVRPIEYGADIVVHSATKFIG
                     GHGTSIGGVIVDGGKFDWEASGKFDSLTKPNPSYHGISFTKACGPAAFVTKVRAILLR
                     DTGATVSPFNAFLFLQGLETLSLRVERHTYNALKVVEYLNNHPQVESVSHPSVSTDPK
                     QQELYKKYFPNGGGSIFTFDIKGDAQKAKDFIDNLELFSLLANVADVKSLVIHPATTT
                     HSQCTEDELLDQGIRPNTIRLSIGCENIDDIIQDLDEAFKAVA"
     gene            complement(2461..3645)
                     /gene="tuf"
                     /locus_tag="ACZPDN_05485"
     CDS             complement(2461..3645)
                     /gene="tuf"
                     /locus_tag="ACZPDN_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR1298107.1"
                     /translation="MAKEKFDRSKTHVNIGTIGHVDHGKTTLTAAITTVLAKKGMAKA
                     EAYDQIDGAPEEKERGITINTAHVEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAASDGPMPQTREHILLARQVGVKYIVVYLNKCDMVDDEELIDLVEMEVRELLNE
                     YGFDGDETPVIRGSALQALQGDPKYEQSIYELMDAVDTWIPDPAREMDKPFLMAIEDV
                     MTISGRGTVATGRVERGQAHLNDEVEIVGIKDTQKTVLTGLEMFHKQLDVAEAGDNIG
                     ALLRGIARDQIQRGQVLAKPGSVHPHTKFKAQVYVLTKEEGGRHTPFVSNYRPQFYFR
                     TTDVTGVITLPEGTDMVMPGDNVVMNVELIAPIAIETGTKFSIREGGHTVGAGNVTEI
                     DA"
     gene            complement(3718..5814)
                     /gene="fusA"
                     /locus_tag="ACZPDN_05490"
     CDS             complement(3718..5814)
                     /gene="fusA"
                     /locus_tag="ACZPDN_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1298108.1"
                     /translation="MAREYALHETRNIGIMAHIDAGKTTTTERILYYSGVNHKIGETH
                     DGASTMDWMEQEQERGITITSAATTAHWKKTDGTGKECRINIIDTPGHVDFTVEVERS
                     LRVLDGAVTVLDAKAGVEPQTETVWRQATEYNVPRIVFCNKMDATGADFLMSCNTIID
                     RLGAKSCPIQLPIGAESDFEAIIDIIKRKAYEFSGDYGRVITEIPVPEDMVDLMEEYR
                     AKLLDYLADYDEDFMMQVLEGEEPSIEEIKRVLRIAVCNGDFFPVLCGSAYKNKGVQL
                     VLDAVVDYLPSPLDVPSIKGTTLDGEPDVRHPGDDQPFSALAFKIATDPFVGKLSFFR
                     VYSGTAKAGSYVLNSTKDKKERFGRIVQMHANARKEITEVYSGDIAAAVGLKVTTTGD
                     TLCDEDHPIILESMEFPEPVIELSLEPKTKADQDKMGLALAKLAEEDPTFKTYTNEET
                     GQTIIAGVGELHLDIIVDRLRREFKVEANVGQPQVAYRETIRQAADCEGKYIRQSGGR
                     GQYGHVWIKFEPNEGKGFEFVDAIVGGSVPREYIKPTQEGLEAALENGMVAGYPVIDV
                     KATLFDGSYHDVDSSEMAYKIAASMALKEAGKKCKPAILEPIMFVEVTAPSEYLGSVM
                     GDISSRRGQITEQEERGNAIIVRAHVPLSEMFGYVTDLRSFTQGRGNYSMKFDHYSEV
                     PKSIAEKIIKENSSEN"
     gene            complement(5833..6303)
                     /gene="rpsG"
                     /locus_tag="ACZPDN_05495"
     CDS             complement(5833..6303)
                     /gene="rpsG"
                     /locus_tag="ACZPDN_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR1298109.1"
                     /translation="MPRKGHIAKRDVLVDPIYNSKLVTRLINKIMIDGKKGVAQTILY
                     NAFEIVEVKTGKAPMEVFEQALENVMPQLELKVRRVGGANYQVPVEVSDERRLTLGLR
                     WIVNYARLRNEKTMEQRLAAEIIDASNGTGASVKKREDTHKMAEANKAYAHYRW"
     gene            complement(6329..6742)
                     /gene="rpsL"
                     /locus_tag="ACZPDN_05500"
     CDS             complement(6329..6742)
                     /gene="rpsL"
                     /locus_tag="ACZPDN_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR1298110.1"
                     /translation="MPTINQLIRKGRKASQNVSKSPALNRGYNSLQSKPTNLSSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNGMEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGTLDCAGVNDRKQSRSLYGTKRPKK"
     gene            6968..7771
                     /locus_tag="ACZPDN_05505"
     CDS             6968..7771
                     /locus_tag="ACZPDN_05505"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1298111.1"
                     /translation="MKHKIVFFDMDGTLYQTENDVIQESALDAIQTLKDKGYIVCAAT
                     GRPLNQMKLILQRVQFDYYVLINGSYILDKDFQEIGSYPILQDNMNDLVNFSKKYETG
                     LMFHFGDATYIYNNFYPMYDFCKYCNVLDSLFYDPSQSYHLRHKAFNAVVLTKDKKLV
                     DQFMETHPNLRNDLINVKTDGFCFDIFNADNDKSKGIEMILEKTGFTWKDAICFGDST
                     NDIHMLKKADIGVAMGNASDFVKSYANFATTSTYDNGIYNAVTKILKDE"
     gene            complement(7824..8378)
                     /locus_tag="ACZPDN_05510"
     CDS             complement(7824..8378)
                     /locus_tag="ACZPDN_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298112.1"
                     /translation="MDEKYTPYNHKGTKIDGVDPKHRGTPAAKRGLSNQQVCIITAIQ
                     RFGHTIARTLNYGKPSPIDLLRFGECLESKFFVMLDGSNSYNELLESKDCTKKVLISH
                     EFYDKFNHLNTVNSFHRLIEEKLQKYKGVASKYINRYNALFVMQRETQGMDNTEKLQY
                     VLLRLKNIYKITRLKDLKNEWLFV"
     gene            complement(8389..8865)
                     /locus_tag="ACZPDN_05515"
     CDS             complement(8389..8865)
                     /locus_tag="ACZPDN_05515"
                     /inference="COORDINATES: protein motif:HMM:NF050000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1298113.1"
                     /translation="MFYCIGVNERETTLMTRTSKYLNQDTIAALQAFQIEKIDREITD
                     FLDTNSKMDTYHFEYCPKCGCYHPRLVKSGFANSGKQMLRCKECGKRFVIDRGQLTFY
                     SHQDQSKWNKLILDTLNGVSLKETAAKINVNERNVFNMRHKVLVSLGTEEHPIHSR"
     gene            complement(9017..9526)
                     /locus_tag="ACZPDN_05520"
     CDS             complement(9017..9526)
                     /locus_tag="ACZPDN_05520"
                     /inference="COORDINATES: protein motif:HMM:NF022152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HIR1298114.1"
                     /translation="MNYIVLGNISSIFAMLFLFLAVAQKDNHKLIAIQGVSYFFFALA
                     GLILKGYSGLVQDIIGFIRNLCIATNKNNIYIRNILLVSGIVFGCILNNKGFLGIFPI
                     IGTFQYSIVSSKKSVSNKHLQLSIILNAVLMIIYSFVIQNYVNMITNTITILISIQTL
                     RKDFASACL"
     gene            complement(9576..10610)
                     /locus_tag="ACZPDN_05525"
     CDS             complement(9576..10610)
                     /locus_tag="ACZPDN_05525"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1298115.1"
                     /translation="MNKDFVKNKVLGSLICAATGDSIGAATENLPFWSIREKYNGELR
                     EFVAPDKSAFAYGNKKGEITDDFSQTYLLAKQIIDNNGVIDKTVVEKMLIQWSDIPHW
                     FNRFAGPTTRFAIENIKSRYAGKGELVRNEVIDYARQATNGAAMKISPAGLFNPCNVE
                     KAIQDACTIASVTHDNDLAIAGACATAAAIAYAFSEDATVDGLIQAAIYGAEEGEKLG
                     SKIRVVGGPNVAERIKLAIKIAESSEAKEKKLEKIYNWVGTGLHISEAVPAALGIIKI
                     CEGDALDSTFEAINIGYDTDTVACICGSMVGALQGVNNEMFDYMSIIDQANDIDIHTL
                     ADEITNIITK"
     gene            complement(10603..>11244)
                     /locus_tag="ACZPDN_05530"
     CDS             complement(10603..>11244)
                     /locus_tag="ACZPDN_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014437.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR1298116.1"
                     /translation="LVSFIFAGLLCNLFWFFGLHGNNLVYNSVLSPIFKSLSLQNLAA
                     FNAGKAIPNLLSEEFAFYFGGFNGSFIAYPVLICIFVFFRKRKELKELGKVALIPGIF
                     SIYEPLVFGMPLMLNPIYLIPMLLTPVIDTLIAVAFMATGLCGYCTGVSVPWTCPMII
                     SGMITTNSIMGGVVQLVQIVVTTALWYVFMKVAIKAEDQKKKDLVTEGDNSNE"
BASE COUNT         3486 a         2227 c         1747 g         3784 t
ORIGIN      
        1 tttttcgaaa ttgacgtttt agtttgtatt ttatttcctg ttcagttttc aaagatcatg
       61 tgcacctcag cagtgctcat ctatattacc aaatacttcc cccttcgtca attgtttttt
      121 tgactttttt tcatcttttt tactgtatac gcttacacca ccgtttttaa aagaaaaact
      181 ggcataaagc cagtcgttta atcaaaaatt aagtattcaa tcttagattc gaatatatga
      241 tggaataaag ctatcactct acctactcct accactgcaa tcactgtccc tattccaaca
      301 ccaacaattt gatgagcaat aaataaacta atacataacg taatacttaa atttaggcaa
      361 tcaaaccaat ttttagttat acctacgctt ttatcaaatc tttccgctaa ggcttgtaca
      421 atcccatctc caggattcgg aacaatttgc atacttaatg acattgctgc acctattcca
      481 gtacaaatta tcgcaaaagt aagtattata aaacgaattc ctaaattttg tgaagttgga
      541 atatatacag taaatatatt caataaacgc gtaaatatta aacttagcgg taactgtagt
      601 atgtctgatg caattctctt atgatttttt aatcgaatat gaattataat ctgaacgacg
      661 acaaacatgg cgtataccac aaaagttaaa tctccaaaat ttaatcctgt aatttgcgat
      721 atactataag gcaccgatat tattggagag actcctagat tcgcctttgt atttaaagtt
      781 agacctaaag ctaatataaa cattccaacc acatacacta aaattcgcaa tcttttttta
      841 tccaatcaaa ccatcctttc aaaaaagcaa tgttattcta tcaaaactat tatattctga
      901 acaagtgtat tttgattttt aaatattcta aaatcatccc attaataaaa agtcagaaac
      961 atattcctga ctttcattca ctatgcgact gctttaaatg cttcatccaa atcttgaata
     1021 atatcatcga tgttttcaca tccaatactt aaacgaattg tatttggacg aataccctga
     1081 tctagcaact catcttctgt acattgagaa tgcgttgtag ttgctggatg aatgactaaa
     1141 gattttacat cggcaacatt agctaataaa gagaataatt ctagattatc aataaaatct
     1201 ttagcctttt gtgcatcgcc cttaatatca aatgtaaaga tagaacctcc tccattagga
     1261 aaatactttt tatacaattc ttgttgttta ggatctgtag aaacacttgg atgagacaca
     1321 ctttctactt gaggatgatt attcaaatat tcaacaactt ttaaagcatt ataagtatga
     1381 cgttctacac gtaaagacaa tgtttctaat ccttgcaaga ataagaatgc attgaaagga
     1441 gaaaccgtag ctccagtatc tcttaaaaga atagcacgta ctttagtaac gaaagccgcc
     1501 ggtccacatg cttttgtaaa actaatgcca tgatagcttg gatttggttt tgttaatgaa
     1561 tcaaattttc cactagcttc ccaatcaaat tttcctccat caacaataac accaccaata
     1621 cttgttccat gcccaccaat aaattttgta gctgaatgaa caacaatatc agctccatat
     1681 tcgattggtc ttactaaata tggtgttgcg aaagtattat ccactactaa tggaatttta
     1741 tgtctatgag caatccttgc gacagattca atatccacta catctgaatt tggatttcct
     1801 aatgtttcaa tatacaaagc ctttgtcttt tcattaatag cattttctac ttctttttca
     1861 ttaaaaatat ctacaaaggt tgtttgaata ccatattgag gtaatgtgtg ttcaaataag
     1921 ttaaatgaac caccataaat attctttgcc gaaacaatat tgtctccatt ttgagctaaa
     1981 tttaaaatcg tataagtaac tgctgctgca ccagatgcta cggctaatgc ggcaacacca
     2041 ccttcaagag ctgctatacg cttttcaaat acatcctcag ttggattggt taaacgacca
     2101 taaatatttc ctgcatcact caatccaaaa cgtgctgcag catgatcaca atttctaaac
     2161 acataagatg aagtttgata aattggaact gcacgtgcat cagtcgttgg atctgcttgt
     2221 tcttgtccta catgaagttg taaagtttcg aatttgaaat ttcgttgatc tctggttatt
     2281 tttgtcataa tcattctcct ttaattcata ctattcttat atgttttgta tgatagtgca
     2341 aaatgattat aatgtcaata aagaaatgta acattttaca ctgcgtttac atttattaca
     2401 ttttcaatca aaaaaaaaga ccgatacaaa tgtatcagtc cttagttctt ataagtttaa
     2461 ttaagcgtca atttcagtaa cgttaccagc acctacagtg tgtccacctt cacggataga
     2521 gaacttagta ccagtttcga tagcgattgg agcgatcaat tcaacgttca taacaacgtt
     2581 gtcaccaggc attaccatgt cagttccttc tggcaatgta attacacctg taacatctgt
     2641 tgtacggaag tagaattgag gacggtagtt agaaacgaat ggagtgtgac gtccaccttc
     2701 ttctttagtt aaaacataaa cttgtgcttt aaatttagtg tgagggtgta cacttccagg
     2761 tttagctaat acttgtccac gttggatttg gtcacgtgca ataccacgta acaatgcacc
     2821 aatgttgtca cctgcttcag caacgtctaa ttgtttgtgg aacatttcca aaccagttaa
     2881 tactgttttt tgagtgtctt tgatacctac gatttcaact tcgtcgttta agtgagcttg
     2941 accacgttca acacgtcctg tagctactgt accacgacca gaaattgtca taacgtcttc
     3001 gattgccatc aagaatggtt tatccatttc acgagctgga tctggaatcc aagtatcaac
     3061 agcatccatc aattcgtaga ttgattgttc gtattttgga tctccttgaa gagcttgtaa
     3121 tgcagaacca cggataactg gagtttcgtc tccgtcgaat ccgtattcgt ttaataattc
     3181 acgaacttcc atttctacta agtcgattaa ttcttcatca tcaaccatat cgcatttgtt
     3241 taagtaaaca acgatatatt taacacctac ctgacgtgct aacaagatgt gttcacgtgt
     3301 ttgaggcata ggtccatcag atgcagcaac tactaagatt gctccgtcca tctgagcagc
     3361 accagtgatc atgtttttta cataatcggc gtggcctgga cagtcaacgt gtgcatagtg
     3421 acgtttttct gtttggtatt caacgtgtgc agtgttgata gtgattccac gttctttttc
     3481 ttctggagca ccatcgattt ggtcataagc ttcagcttta gccatacctt ttttagctaa
     3541 tactgttgta atagctgctg ttaatgttgt tttaccgtgg tcaacgtgac cgatagtacc
     3601 aatattaacg tgagttttac ttctgtcaaa tttttcctta gccatttata atttcctcct
     3661 aaatgctaac atatttcata caatagatat tgtatttcag aaccaagcga aaatcaacta
     3721 gttttcgctt gaattctctt taataatttt ttctgcaata ctctttggaa cttctgaata
     3781 gtggtcgaac ttcatagagt agtttccacg tccctgagta aagctacgta agtctgtaac
     3841 atatccgaac atttctgaca atggaacatg tgcacgaacg ataatggcat ttccacgttc
     3901 ttcctgttca gtgatttgtc cacgacgact tgaaatgtca cccataacgc tacctaagta
     3961 ttcacttggt gcagttactt ctacgaacat aattggttct agaatagcag gtttacattt
     4021 ctttccagct tcttttaatg ccatactagc ggcaatctta taagccattt cactagagtc
     4081 gacatcgtgg tatgatccat caaataatgt agctttgaca tcgattactg ggtaacctgc
     4141 aaccatacca ttttccaatg ctgcttccaa accttcttga gttggtttga tatattctct
     4201 aggtacgcta ccaccaacga tagcatcaac gaattcaaat ccttttcctt cgtttggttc
     4261 gaatttgatc catacgtgac catattgtcc acgaccacct gactgacgga tatattttcc
     4321 ttcacaatca gcagcctgac gaattgtttc acggtaagca acttgtggtt gtccaacatt
     4381 tgcttcaact ttaaattcac gacgtaaacg gtcaacaatg atatccaagt gaagttcacc
     4441 tacaccggca atgattgttt gtcctgtttc ttcgttagta tatgttttga aagtagggtc
     4501 ttcttctgca agttttgcaa gagccaatcc cattttgtct tggtcagctt ttgtttttgg
     4561 ttccaaagac aattcgatta ctggttcagg gaattccata gattcaagaa tgattgggtg
     4621 atcttcatca cacaatgtat cacctgttgt agtaaccttt aaaccaactg cagcagcaat
     4681 atcacctgaa taaacttcag tgatttcttt acgtgcattg gcatgcatct gaacgatacg
     4741 tccgaaacgt tcttttttat ctttagttga gtttaataca tagcttcctg cttttgcagt
     4801 acctgaataa acacggaaga aagataattt tccaacgaat gggtctgtag cgatcttaaa
     4861 tgctaatgca gagaatggtt gatcatcacc agggtgacga acatctggtt ctccatctaa
     4921 tgtagtaccc ttaattgaag gtacatctaa tggacttggt aagtaatcta ctactgcatc
     4981 caatactaat tgaacacctt tgttcttata tgcagatcca cacaatactg ggaagaagtc
     5041 accattacat acggcaatac gtaatacacg tttgatttct tcgattgaag gttcttcacc
     5101 ttctaatact tgcatcatga aatcttcatc ataatctgct aaatagtcta acaatttagc
     5161 acggtattct tccataagat ctaccatatc ttctggaact gggatttcag taattacacg
     5221 accgtaatct ccactaaatt cataagcttt tctcttgata atatcaatga tagcttcgaa
     5281 gtctgattca gcaccgattg gtaactgaat tggacaagat ttagctccta aacgatcgat
     5341 gattgtattg catgacatta agaagtcagc acctgtagca tccattttgt tacagaatac
     5401 aatacgtgga acgttatatt cagtagcttg acgccaaact gtttcagttt gaggttcaac
     5461 acctgcttta gcatctaata ctgttacggc accatctaat acacgtaaac tacgttctac
     5521 ttctactgtg aagtctacgt ggcctggtgt atcgatgata ttgatacgac attctttacc
     5581 agttccatct gtctttttcc agtgagctgt tgtagcggca gaagtgattg tgataccacg
     5641 ttcttgttct tgctccatcc agtccattgt actagctcca tcatgtgttt caccaatctt
     5701 gtggtttaca ccagagtagt aaagaatacg ttctgttgtt gttgtttttc cggcatcgat
     5761 gtgagccatg ataccgatgt tacgagtttc gtgtaaagca tattctctag ccatatttct
     5821 ttactccttc tcttaccaac ggtagtgagc ataagccttg ttagcttctg ccattttgtg
     5881 agtgtcttca cgttttttaa cactggcacc agttccatta gaagcatcaa taatttcagc
     5941 tgctaatctc tgttccattg ttttttcgtt acgtaaacgt gcgtagttta cgatccaacg
     6001 aagtcctaaa gtcaaacgac gttcgtcgct tacttcaact ggtacttggt agttggctcc
     6061 acctacacgt ctaactttca attccaattg aggcataacg ttttctaaag cttgttcgaa
     6121 aacctccatt ggtgcttttc ctgtttttac ttctacgatt tcaaacgcgt tgtacaaaat
     6181 agtttgtgca accccttttt taccatcaat catgattttg ttgatcaaac gagtaaccaa
     6241 cttagagtta taaattgggt cgactagtac gtcacgtttc gcaatgtgtc cttttctagg
     6301 catctaattt cctcctttca cgattgatct attttttagg tcttttagtt ccgtataatg
     6361 aacgagattg tttacggtca tttacaccag cacagtccaa agttccacga acgatgtggt
     6421 aacgaacacc tggtaagtct ttaacacgtc caccacgaat caatacaaca ctgtgttcct
     6481 gtaagttgtg tccgatacct ggaatgtaag ctgtaacttc cataccatta cttaaacgaa
     6541 cacgcgcata tttacgaagt gctgagtttg gtttcttagg tgtcatagta ccaacacgtg
     6601 tacaaactcc acgtttttgt ggacttgata ggtttgttgg tttagactgc aatgagttgt
     6661 aaccacggtt caaagctgga gactttgaaa cgttttgact ggccttacga cctttacgaa
     6721 tcaactggtt gattgtaggc ataagatcct cctttcaaaa ttttcaattt ccaagcaaac
     6781 gaatccgggt gggtcttacc catccattaa atataggctc ttttcagaac ctattttcgc
     6841 atgcaatata aagatacagt attcgagatg atttgtcaat tcttttttat tgattttttt
     6901 ataataaatt tttatacgca ttccccattc attaaagtat aatatagaca tgaatagagg
     6961 taagtttatg aaacacaaaa ttgtattttt cgacatggac ggaacacttt accaaaccga
     7021 aaatgacgtt attcaagaaa gcgcattaga cgctatacag acactaaaag ataaaggata
     7081 tattgtctgt gcggctactg gccgcccctt aaatcaaatg aaacttatat tacaacgcgt
     7141 tcaatttgat tattatgtat tgatcaacgg aagttacatt ctagacaaag acttccaaga
     7201 aatcggaagc tatccaatct tacaagacaa catgaatgac ttagtcaatt ttagcaaaaa
     7261 atacgaaacc ggtctaatgt ttcactttgg cgatgctaca tacatttaca acaactttta
     7321 cccaatgtat gacttctgta aatactgtaa cgtgctagat tcactgttct acgatccaag
     7381 ccaatcttat catttaagac acaaagcctt taatgccgta gttcttacta aagataaaaa
     7441 attagttgac caattcatgg agactcatcc aaatctaaga aacgatttaa ttaatgttaa
     7501 aaccgatgga ttctgcttcg atatcttcaa tgcagataac gataaatcaa aaggtattga
     7561 aatgattctt gaaaaaacag gattcacatg gaaagatgcc atctgttttg gagacagtac
     7621 aaatgatatc cacatgttaa agaaagccga tattggcgtt gcgatgggaa atgcaagtga
     7681 ttttgtcaaa agctatgcta actttgctac aacatctaca tacgataacg gaatctataa
     7741 tgcagtcaca aaaatattaa aagatgaata aagtaggctt aactagcaat ttggtgacat
     7801 tgttataatt ctgtttttat cacttacacg aataaccatt cgttcttcaa atcctttaaa
     7861 cgagtgatct tataaatgtt tttcaaccta agcaatacat actgtagttt ttccgtgtta
     7921 tccattccct gggtttctct ttgcatgaca aaaagagcgt tgtatcgatt tatatatttt
     7981 gatgctactc ctttgtattt ttgcaacttt tcttcaatca atctgtgaaa gctgttcact
     8041 gtgtttaaat gattgaactt atcataaaac tcatgactga ttaacacttt ctttgtgcag
     8101 tccttacttt ctaaaagttc gttgtaactg ttagatccat ctagcattac aaaaaatttt
     8161 gattctagac attcaccaaa tcttaataga tctatgggtg atggcttgcc ataatttaat
     8221 gttctggcaa ttgtatgtcc gaatctctgt attgcagtga tgatacaaac ttgttgattc
     8281 gataatcctc tttttgcagc aggcgttcca cgatgtttag ggtctactcc atcaatctta
     8341 gttcctttat gattgtatgg agtatacttt tcatccattt caacttcatt acctactgtg
     8401 tatgggatgt tcctcagttc ctagactaac caatacttta tggcgcatat taaaaacatt
     8461 gcgttcattt acattgattt tagcagctgt ttctttaaga gatacgccat tcaatgtatc
     8521 taaaataagt ttgttccatt tagactgatc ttggtgagaa taaaaagtaa gctgaccgcg
     8581 gtcaataaca aaacgttttc cgcattcttt acaacgaagc atctgtttgc cagagttggc
     8641 aaaaccgctc ttaaccaaac gaggatgata acatccgcat ttaggacagt attcaaaatg
     8701 ataagtatcc attttactgt ttgtatctaa aaagtctgtg atttcacgat caatcttttc
     8761 gatttgaaaa gcttgtaaag cagcaattgt atcttggttt aaatattttg aagttcttgt
     8821 cataagtgta gtctctcttt cgtttacacc tatacaataa aacataagct gacaccatta
     8881 atgtcaatta tattagcaag tcaccaatgt atctgttaag ccaagtaaat tcaacaaaaa
     8941 agcaattaaa tgcttctcaa catatgcatt atggagctga ttcaaaacct tttttaagcc
     9001 gaataagata taaaaattat aaacatgctg aagcaaagtc ttttcgaagg gtctgaatag
     9061 atatcaagat tgtaattgtg tttgtaatca tattcacata attttggatt acaaagctgt
     9121 atataatcat tagtactgca tttaaaataa ttgacaactg aagatgctta ttgctaactg
     9181 attttttgga tgaaacaatt gaatattgaa aagttcctat gataggaaaa atgcctagaa
     9241 atcctttgtt gtttaaaatg catccaaata caattccact aaccagcaaa atgtttcgta
     9301 tgtatatgtt attcttattt gtagctatac atagattcct aataaaacca ataatgtctt
     9361 gaacaagtcc actatatcct tttaagatca ggccagccaa ggcaaagaaa aaataagata
     9421 ctccctgaat tgcaattaat ttatgattat ctttctgggc tactgccaaa aacaaaaata
     9481 acatggcaaa tatggaacta atatttccta atactatata attcatgatt tccctctaaa
     9541 aaatcaggtg gtgaatttat tcaccaccat tttctttatt ttgtgataat attcgttatt
     9601 tcatctgcaa gagtatgaat atcaatatca ttggcctgat caatgatact catataatca
     9661 aacatttcat tattcacacc ttgtaatgca ccaaccattg atccgcaaat acatgctaca
     9721 gtatcagtat catatccaat attaattgct tcaaatgttg aatctagtgc atctccttca
     9781 cagattttaa tgattcctaa agcagctggt acggcctcag aaatatgtaa tcctgtgcct
     9841 acccaattat aaattttttc taattttttc tcttttgcct cactactctc tgcgatttta
     9901 atagccaact ttattctttc tgctacattt ggtccaccta cgacacgaat tttactacct
     9961 aatttttcac cttcttcagc tccataaata gcagcttgaa ttaatccatc tactgtagca
    10021 tcttcactaa atgcataggc tattgcagct gcagttgcac aggccccggc tatagccaaa
    10081 tcattatcat gtgttacaga tgcaatagtg catgcatcct gaatagcttt ttccacatta
    10141 caaggattaa acaaaccagc tggggaaatt ttcatggccg caccatttgt ggcctgtcgt
    10201 gcataatcta taacttcatt acgtacaagt tctccttttc cagcatatct tgatttaatg
    10261 ttttcaatcg caaatcttgt ggttggacct gcaaaacgat taaaccaatg aggaatatct
    10321 gaccactgaa taagcatttt ttcaactact gtcttgtcaa taactccatt attatcaata
    10381 atctgttttg ctaataaata agtttgagaa aaatcatctg taatctcacc ttttttattt
    10441 ccatatgcaa atgcactttt atccggtgca acaaactctc ttaattcacc attgtatttc
    10501 tcacgaatag accagaatgg taaattttct gtggctgcac caatactatc tccagtcgca
    10561 gcacaaatca aactacctaa tactttattt ttaacaaaat ctttattcat ttgaattgtc
    10621 tccttctgta acaagatctt tcttcttttg atcttcagct ttaatcgcta ctttcataaa
    10681 tacataccat aaagctgtag ttacaacaat ttgaaccaac tgtactactc cacccatgat
    10741 tgaatttgtt gttatcatac ctgaaataat cattggacaa gtccatggaa cactaacacc
    10801 tgtacaatat ccacacagac cagtagccat aaaagcgacc gcaattaatg tatcaatcac
    10861 tggtgtcaac aacattggaa tcaaataaat tggatttaac attaaaggca taccaaatac
    10921 caatggttca tagattgaga aaatacctgg aatcaaggct acttttccta attcttttaa
    10981 ttctttacgt tttctaaaga atacaaagat acagattaat acaggatacg caataaacga
    11041 tccattgaat ccaccaaaat agaatgcgaa ttcttcagat agcaaatttg gaatagcttt
    11101 gcctgcatta aatgcagcta aattctgtaa agataaagat ttaaaaattg gagaaagaac
    11161 tgaattataa accaaattat ttccgtgtaa tccaaagaac cagaataagt tacataataa
    11221 acctgcaaaa atgaatgata ctag
//
LOCUS       DBMDWL010000060        11242 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_1991_length_11242_cov_2.633426, whole genome shotgun sequence.
ACCESSION   DBMDWL010000060 DBMDWL010000000
VERSION     DBMDWL010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1991_length_11242_cov_2.633426"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(92..550)
                     /locus_tag="ACZPDN_05535"
     CDS             complement(92..550)
                     /locus_tag="ACZPDN_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298117.1"
                     /translation="MKLRRRHKKQHGTKTTLGLVIILVLILLVIWSEFISGPNRVKEQ
                     IYTQRVEKIEKQNKHIKGISEHVFDYTTYQGYDDTTLYWYNAKSELITSRDIETLNYK
                     KAKRIAKKNYKIKCESVVLGYGYNNPVYEIRGSNKMILLDYDSFERVYES"
     gene            complement(570..1250)
                     /locus_tag="ACZPDN_05540"
     CDS             complement(570..1250)
                     /locus_tag="ACZPDN_05540"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIR1298118.1"
                     /translation="MIGIVAAMDAEIHEFEKRMDLVDHTKTIAGCTLTYGKWQGKDVV
                     LCKSGVGKAYAAMSCTILCMQGNIDSIINVGTAGGLTQDEQVLDIVISDKVVQADYDT
                     SPLDGPSGVGLVYNSHEKLSQSCIDAAKAMQIRYHVGTIASQDLFMSRDEDFEKLMNN
                     FPDSICSEMEAGAIGAVATKFNIPFVIVRCLSDVVHHNENPMEFSTYVSKASAQSAKL
                     IENWLMRI"
     gene            complement(1247..1879)
                     /locus_tag="ACZPDN_05545"
     CDS             complement(1247..1879)
                     /locus_tag="ACZPDN_05545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR1298119.1"
                     /translation="MESELAPCDCLELACGSGEITEMLAQKGYTVTALDLSEEMVKQA
                     KSKNLEGSIEFLCQDMRHLNNLGSYSGIFCLCDSFNYILEKEEIDAFFHEVSQHLKAN
                     GLFFFDTHSLDRLEEFNSEYNETGAFEDGCQYQWSIMAEDDYVYQDFAFYMKDKVIQE
                     HHIQRVYDPQWLAKTLSKYFDILKVTTDFDIEGIAPGEKYFYVCRKKVQE"
     gene            complement(1947..2285)
                     /gene="rsfS"
                     /locus_tag="ACZPDN_05550"
     CDS             complement(1947..2285)
                     /gene="rsfS"
                     /locus_tag="ACZPDN_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR1298120.1"
                     /translation="MEILDIVVHAICEKKAQDVRVYDVTSLTPFMDSMIVCSTTNIRQ
                     NNAIAQNIKDRLREAGYMGDMHIEGDSGSKWLLIDLKEVVVHLFVNEERQVYNLDRLY
                     SDVPVKTYDL"
     gene            complement(2288..3304)
                     /locus_tag="ACZPDN_05555"
     CDS             complement(2288..3304)
                     /locus_tag="ACZPDN_05555"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF005519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR1298121.1"
                     /translation="MRIAIVGGSFDPIHNGHIQMANQSLQALQVDEVWFMPTSSTPLK
                     DRELTLDQERLAMIDLVVQKDSRFKVCTLELERAGKSYTYDTLKKLIETYPEHEFYWI
                     IGNDQLEQFDKWYHAEKLVKMAHFVCFDRNGKLADSKYDIMCMTMPSVPVSSSEIRMG
                     NKLNYLPKEVLRYIYRNRLYVKDFVASRLTEKRYKHSLSVAKLCEEFALNNGYDMHIA
                     YYIGLFHDIAKYMPKDEMVKWMNSICPENMEYPVAVWHGFVGAAVTKDIFLIDDPIVY
                     NAIYHHVLGTSQDPYAMMVFCADKLDPLRGYDSSKLIEICNKDIKKGFDLVVQQNREY
                     LKRG"
     gene            complement(3301..3591)
                     /locus_tag="ACZPDN_05560"
     CDS             complement(3301..3591)
                     /locus_tag="ACZPDN_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HIR1298122.1"
                     /translation="MLTSQNKKYLRKLGMDYKSLFQIGKDGLSDNLMDTLSDSLEAHE
                     LVKCNLLKTCPVDVREAAIECARATGSEVVYIVGHTFLLYRRSKENKLGLKK"
     gene            complement(3591..4670)
                     /locus_tag="ACZPDN_05565"
     CDS             complement(3591..4670)
                     /locus_tag="ACZPDN_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR1298123.1"
                     /translation="MTKYCKGCGVLLQNEDEKALGYVPTLDASYCQRCYRIRHYGDVT
                     INMQQGIESNATLEKINKIDGVVFWVVDLFAFEANLISRLNQKLPGKDIVMVLTKRDV
                     LPTTLTDEKIINFVKHRLHEENIIVKDIVICGYLLKENDKSREYSSRIVNAILKYRKN
                     RNVIFMGVANAGKSTVLNHLLATKDLTTSQNPGTTLDLVPIPFEDYTIYDTPGIENTH
                     SLLTYLQPKDLKTVIPTKPIRPFVSQIFEDQSFAAAGLARLDVVVSGKATVVGYFSRS
                     LSIHRGKLSNADALWQKHLNGMLVPCVDTSLLTMHTFHAPKLEDEKMDVCIHGIGWFC
                     ISGQVKDVYVKVHKGIQVTFRKAMI"
     gene            complement(4663..5187)
                     /locus_tag="ACZPDN_05570"
     CDS             complement(4663..5187)
                     /locus_tag="ACZPDN_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR1298124.1"
                     /translation="MNIFAPDYYVQSFKTLNIRKLHEMGIQLLLCDIDNTLVAYDEEV
                     PSQDVVDFIHKLKRNGIEVALCSNSPSSRGKNFGKHLPVSKTYPFSCKPFPFCFKKAM
                     KDHGLKASQIAILGDQMYTDILGGNIWGLYTILTAPIAIKDRNITKVFRFFEELIYGY
                     LEKKKILKRGEFDD"
     gene            complement(5187..6758)
                     /locus_tag="ACZPDN_05575"
     CDS             complement(5187..6758)
                     /locus_tag="ACZPDN_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1298125.1"
                     /translation="MIRLFQVKCASENEIEAQILKKLRIRKEDLLQWHVHRKSVDARH
                     QKVLFSYVVDCKVKNEKKLLKLKDVSLTPDETYHPVMSGEHALSARPIVVGFGPAGMF
                     AALILAQYGYKPIVLERGSNVDVRKQKVETFWENGVLDEECNVQFGEGGAGAFSDGKL
                     TTRSKDALCRKVLEELVSFGAKPDILMDQHPHIGTDAFLSIIRNCRKEIERLGGTFYF
                     DTKLEDIKVENGILKQIYFNHQWMDCNALILATGHSASDTILMLHKHGMHIENKPFAV
                     GVRIEHKQKYINEAMLHEYSQDERLIPARYQLTYTASNSKGVYTFCMCPGGYVIPSSC
                     QKEQLVVNGMSYASRSGENANSALLVQINDSDYGNELFAGLKYQEELERKAFLMGQDY
                     KACVQLAKDYLENRVSKQFESVLPTYSIGTTFCDLNDLFSKEVNLALHEALEHFEKKV
                     PGFVSSGAILSAVESRSSSSVRLVRDKENYVSNIQGLYPSGEGSGYAGGIMTSAIDGI
                     RSAEHLIEYFAKGDF"
     gene            complement(6763..7776)
                     /locus_tag="ACZPDN_05580"
     CDS             complement(6763..7776)
                     /locus_tag="ACZPDN_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1298126.1"
                     /translation="MKRQYASIDIARYVSALLVVCIHTFPFLEISETFNTYFIHTVCR
                     LAVPFFFTTSGFFFFRNYDLENEDVNDARLKKALFRLFRIYLIWTIIYLPYTIFDYTH
                     NGFRISYLFTYVRDFIFNGSYYHLWFLPALMLADVIVYYLYKKKGLKFTLIITFAFYV
                     VGYLINVYTPVWESLPYISFLFGFFTKALSTARNGIFFGPMFIAIGLLLSRTRRLPKK
                     VSLLAFLISFVLLFCEVALYRHFNILRDLTSMYICLVPAVYFLVNYLLKVNIPYRKMF
                     TILRHDSLMIYTSHILFAKIFLALMPHAHLVVYFLTLACAQCLAGFVTRYQDQYPILK
                     KLL"
     gene            complement(7773..8924)
                     /gene="alr"
                     /locus_tag="ACZPDN_05585"
     CDS             complement(7773..8924)
                     /gene="alr"
                     /locus_tag="ACZPDN_05585"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1298127.1"
                     /translation="MLEAYSRAWTEIDYDAIRHNVNEVRKLVKNTKIMGIVKANAYGL
                     GDVACAKALQKCGVDYFGVSSIDEALNLRNAGIQDNILILGYTPSMHFHYLHEQNIVQ
                     SLVSIEYARELNAYAKENDVKIRCHCKVDTGMCRTGIVYQEQDKHIEDIYEEYRLENL
                     CVEGIFSHFPVSDCLDEDCTEFTKKQIQLFDEVIDLLKSQGIDPGIRHLQNSYGILNY
                     PELEYEYCRPGLLYMGVTSDDHIKIKTNPDFIPIMSMYANVSLVKWIYPGQSVSYGRH
                     FIAEKPTKVATMSIGYADGLPRLLSNQGFEVLVHGQRCKVIGNLCMDQCMIDVTEVED
                     VKEGDEVCIVGHQGNEVVTVDQISRMAKTINNETLTSISSRVPRLKVRS"
     gene            complement(8918..9973)
                     /locus_tag="ACZPDN_05590"
     CDS             complement(8918..9973)
                     /locus_tag="ACZPDN_05590"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1298128.1"
                     /translation="MSKLRLGVVFGGQSSEYSVSLHSVTSFLRQIHADNYEMTLIGIN
                     KNGHFYLYNGDVDSIEHDTWENEETCVSCAWVNGGIMPLDGSNKVIELDCVFPVLHGK
                     NGEDGLIQGLLELMNIHYVGCDVMSSAMSMDKEIMHILCKEAKIPCADYICLYANREN
                     PTFEQIQEEIALPWIIKPCNAGSSYGVHFVNNKEEFESACKDAFFYDGRGKILVEKVI
                     KGFEIGCAVMGNEEIFTGSVDEIEITGGFFDFEGKYEMKGAAIYCPARIDEKTFKEAQ
                     ELARKTYIAMNCCGMARVDMFVTEDKEVILNELNTIPGLTATSRYPSMMKEAGVEFAD
                     LIDKLIELAMQRTIGAC"
     gene            complement(9966..>11242)
                     /locus_tag="ACZPDN_05595"
     CDS             complement(9966..>11242)
                     /locus_tag="ACZPDN_05595"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1298129.1"
                     /translation="GVVIDSRQVTKDVLYVPIIGQRVDGHTFAKQTIEDGALISLWQK
                     DHVPYPDFPVILVDDTLKAMQDLSKAYLDELHPLVIGVTGSNGKTSCKDMLYSIFSQE
                     KKTQKTQGNRNSEIGLPLTIFDFDEDIEVAILEMGMENKGEITQLTSIAQPDISVITS
                     IGSAHMENLGGKLQIAQAKCEILEGTKENGLFLYNYESKEIHEVLCTLDTENKKVVSF
                     GQGGDVSITSDIEYDKEGIVFNCNILDTPVHLRAFGDFQAMNALPCIYIAKACHLKEE
                     SILNGLKNLEMTKMRTQLISVKNAYILDDTYKSNPESAKAAIDTLMELPANKHIAILS
                     DMLDLGSEEKQLHYDVGVYAKEKGVDELYCTGPLSKETADGFNGKWYETKDEIYEACK
                     PLLEKDCIIMIKGSRAMKMDTLVDAFRGKNNE"
BASE COUNT         3661 a         2422 c         1441 g         3718 t
ORIGIN      
        1 attttcaaag atctatttaa aactaactaa aaaatctagt tatttttgta ttttcaaact
       61 ttttcataaa aaatacttga attttatata gttagctttc ataaacacgt tcaaaactat
      121 cataatctaa taaaatcatt ttattcgaac cacgaatctc atataccggg ttgttgtagc
      181 catacccaag tacaacggat tcacatttga tcttataatt ctttttcgca atacgctttg
      241 cctttttata attcaaagtc tcaatatctc ttgatgttat caattcagac ttcgcattat
      301 accaatataa agtcgtatca tcatatcctt gatacgttgt ataatcaaag acatgctcac
      361 taattccttt tatatgttta ttttgtttct ctattttctc aactctttgc gtataaatct
      421 gttccttaac acgattcgga ccactgataa actcagacca aatcacaagt aatataagaa
      481 caagaatgat aactaatcca agtgtcgttt ttgttccatg ttgtttttta tgtctacgcc
      541 ttagtttcat ccttattcct ccttttttac tatatacgca tcaaccagtt ttcaattaat
      601 ttagccgatt gtgcacttgc cttagaaaca tatgtagaaa attccattgg attttcatta
      661 tgatgcacaa catcacttag acaacgcaca atcacaaatg gaatattaaa ttttgtggca
      721 accgcaccaa tcgctcctgc ttccatttct gaacaaatcg aatccgggaa attattcatc
      781 aacttttcaa aatcctcatc tctagacata aataagtctt gagaagcaat cgtacctaca
      841 tgataacgaa tctgcatagc ctttgcagca tcaatacaac tttgagataa tttttcatga
      901 ctattataca ccaatccaac acctgaagga ccatctaatg gacttgtatc ataatcggct
      961 tgtacaacct tatcactaat cacaatatcc aacacttgtt catcctgtgt tagtccacca
     1021 gctgtaccta cattgataat gctgtcgata tttccttgca tacacaaaat tgtacaagac
     1081 atagccgcat aagccttacc tacaccactt ttacataaga ctacatcttt accttgccat
     1141 tttccataag ttaatgtaca tcccgcaatt gtcttcgtat gatctactaa gtccatacgt
     1201 ttctcaaatt catggatttc agcatccatt gccgctacaa taccaatcat tcttgtacct
     1261 tctttctaca tacataaaaa tacttttcac caggcgcaat cccctcaata tcaaagtcgg
     1321 ttgtgacttt taaaatatca aaatactttg ataaagtctt agccaaccat tgtggatcat
     1381 ataccctttg aatatgatgc tcctgaatta ctttgtcttt catataaaac gcaaaatctt
     1441 gatatacata atcatcctct gccatgattg accattggta ttgacaacca tcttcaaaag
     1501 cacctgtctc attatattca gaattaaact cttctaaacg atccaaagaa tgtgtatcaa
     1561 agaagaaaag tccatttgct tttaaatgtt gactgacttc atgaaaaaat gcatcaattt
     1621 cttctttttc taaaatatag ttaaaactat cacacaaaca gaaaataccc gaataagatc
     1681 ctaaattatt taaatgacgc atatcctgac ataaaaactc aatcgaaccc tctaagtttt
     1741 tactttttgc ctgtttcacc atttcctcac ttaaatcaag agctgtcaca gtatagcctt
     1801 tttgcgcaag catttcagtg atctcaccac taccacacgc aagttctaaa caatcgcaag
     1861 gtgcaagttc actctcaatc caagaaacct attctctaga agcttcttca tcttttacta
     1921 aagcatcata ataatgtgcc aatgtattat aaatcatacg tttttactgg aacatccgaa
     1981 tataaacggt ccagattata aacctgcctt tcctcattca caaataaatg gactaccact
     2041 tcttttaaat caatcaaaag ccattttgaa ccagagtccc cctcaatatg catatccccc
     2101 atatatccag cttctcttaa acgatcctta atattttgcg caatcgcatt attctgacga
     2161 atattcgtag tcgaacaaac aatcattgaa tccataaaag gagtcaaact cgttacatca
     2221 tatacacgta catcttgtgc tttcttttca cagatcgcat gaactacaat atctaaaatc
     2281 tccatgttta acctctcttt aaatactcac ggttttgttg aaccaccaaa tcaaatcctt
     2341 ttttaatatc tttgttacaa atttcaatca acttactaga atcatatcca cgcaaaggat
     2401 ctaatttatc tgcacaaaat accatcatcg cataaggatc ttgacttgtt ccaagtacat
     2461 gatgatagat tgcattatat acgattggat catcaatcaa aaagatatct ttcgtaacag
     2521 cagcccctac aaaaccatgc catacagcta caggatattc catgttttca ggacaaatac
     2581 tattcatcca ttttaccatc tcatcctttg gcatatattt agctatatca tgaaacaaac
     2641 caatataata cgcaatatgc atatcatacc cattgttcaa cgcaaactct tcacaaagtt
     2701 tggcaaccga taaagaatgt ttatatcttt tttctgttaa cctagaagcc acaaaatcct
     2761 tcacatacaa tcgatttcga tagatataac gtaaaacttc ttttggcaaa taattcaatt
     2821 tatttcccat acgaatttct gaacttgaaa ctgggaccga tggcattgtc atacacataa
     2881 tgtcatattt tgaatctgca agcttgccat tcctatcaaa acacacaaaa tgagccatct
     2941 taacaagttt ttcggcatga taccacttat caaactgctc caattgatcg tttcctatga
     3001 tccaataaaa ctcatgttcc ggataggttt caatcaattt ttttagcgtg tcatacgtat
     3061 agctttttcc tgctcgttca agttcaagtg tacatacctt aaatcttgag tctttctgaa
     3121 ccactaaatc aatcatagcc aaacgctctt gatccaatgt cagttcacga tcttttaaag
     3181 gcgtacttga agttggcata aaccacactt catcgacttg taaagcttgt aaagactgat
     3241 tggccatttg aatatgccca ttatggattg gatcaaaaga cccgcctaca atggcaattc
     3301 tcatttttta agccccaatt tattttcttt acttcttcta tacaataaga aagtatgtcc
     3361 aacaatataa accacttcac tacctgttgc ccttgcacat tcaatagctg cctcacgcac
     3421 atccactgga catgtcttta ataaattaca ttttactaat tcatgtgcct ctaatgaatc
     3481 ggataaagta tccattaagt tatcactcaa accatctttt ccaatctgga ataatgattt
     3541 ataatccatt cctaattttc ttaaatattt tttattttga ctcgttaaca ttaaatcatc
     3601 gcctttctaa acgttacttg aattccctta tgtactttta catatacatc ttttacttgt
     3661 ccactaatac agaaccatcc aataccatga atacaaacat ccatcttttc atcctcaagt
     3721 ttaggtgcat ggaacgtatg catcgttaat aaggaagtgt ccacacaagg cactaacatg
     3781 ccatttaaat gtttttgcca caaggcatcc gcattactta attttcctct atgaatcgat
     3841 aaagagcgag agaaatatcc aactactgtg gccttaccac ttaccactac atctagtctt
     3901 gccaaacctg cagccgcaaa tgactgatct tcaaagattt gtgaaacaaa tggtcgaatt
     3961 ggctttgtcg gaatcactgt ctttaaatct ttcggttgaa gatacgttaa taaagagtgc
     4021 gtattctcaa tgcctggcgt atcataaatc gtataatcct caaaaggaat tggaactaaa
     4081 tccagtgttg ttcctggatt ttgagaagta gttagatctt ttgttgccaa taaatgattc
     4141 aatacagtcg atttacctgc attggcaaca cccataaaaa tcacatttcg atttttacga
     4201 tattttaaga tcgcattcac aatacgcgat gaatattctc tagatttatc attttctttt
     4261 aatagataac cacaaatcac aatatccttc acaataatat tttcttcatg aagacgatgc
     4321 ttcacaaagt tgatgatttt ttcatccgtt aaagttgtcg gcaaaacatc acgcttcgtt
     4381 aataccatga caatatcttt accaggaagt ttctgattca agcgcgaaat caaattcgcc
     4441 tcaaacgcaa ataaatctac gacccagaat acaaccccat caatcttgtt gattttttcc
     4501 aatgttgcgt tggattcaat cccctgctgc atattgatcg taacatcccc atagtgacga
     4561 atacgataac atctttgaca ataagaagca tctaaagtag gtacataccc aagtgctttt
     4621 tcatcttcgt tttgtagtaa aacaccacaa cccttacaat atttagtcat caaattcacc
     4681 tcgcttcaat atttttttct tttctaaata accatatatt aattcttcaa agaaacgaaa
     4741 cacctttgtg atatttcgat cctttatcgc aattggagcc gttaatatcg tatatagacc
     4801 ccaaatattt ccacccaata tatctgtata catctgatcg cctaaaatcg caatctgact
     4861 ggcctttaag ccatgatctt tcattgcctt tttaaaacaa aaaggaaatg gtttacacga
     4921 aaatggatac gtcttagaca ctggaagatg cttgccaaaa ttcttacccc ttgaactcgg
     4981 tgaatttgaa caaagcgcaa cttcaattcc attccttttt agcttatgaa taaaatcaac
     5041 cacatcttga cttggaactt cttcatcata cgctaccaat gtattatcaa tatcacaaag
     5101 taacaattga attcccattt catgaagctt tctaatattc aaagttttaa aactctgaac
     5161 gtaataatcc ggtgcaaaaa tattcattag aaatcccctt tggcaaaata ttcaatcaaa
     5221 tgctcagcac tacgaatacc atcaattgca cttgtcataa tgccacctgc atagccacta
     5281 ccttctccac tcggatatag accttgaata ttggatacat aattttcttt atcacgtacc
     5341 aaacgcacac tgctgctgct tcttgattcc acagcactta gtattgctcc actcgaaaca
     5401 aagccaggca ccttcttttc aaaatgttct aaagcttcat gtaaggctaa attgacttct
     5461 tttgagaata aatcattcaa atcacagaac gttgtaccta tcgaataggt tggtaatacc
     5521 gattcaaatt gtttagacac tctattctct aaataatcct tggctagctg cacacaagct
     5581 ttatagtctt gtcccatcaa gaaagctttt ctttctaact cttcctgata ttttaaaccg
     5641 gcaaacaact cattgccata atccgagtca ttgatttgca caagtaaagc actattggca
     5701 ttttcgccac tacgactcgc atacgacatg ccgttcacaa ccaattgttc tttttgacat
     5761 gagcttggga tcacatagcc acctggacac atacaaaatg tatatacacc tttcgagttg
     5821 gaagctgtat atgtcaattg ataacgtgca ggaatcaatc tttcatcttg actatactca
     5881 tgaagcatag cttcatttat atatttttgt ttatgttcaa tacgaacacc taccgcaaat
     5941 ggtttatttt cgatgtgcat accatgttta tgaagcatta aaattgtatc actcgcacta
     6001 tgtccagttg ctaaaatcaa cgcattacaa tccatccatt gatgattgaa ataaatttgt
     6061 tttaagatac cattttctac tttgatatct tctaatttcg tatcaaaata gaaagttcca
     6121 cccaatcttt caatttcttt ccgacaattt ctaataatgg acaaaaaagc atctgtacca
     6181 atatgagggt gctgatccat caatatatct ggttttgccc caaaagaaac aagctcctcc
     6241 aagactttgc gacataaagc atcctttgat cttgtcgtta gttttccatc actaaaggca
     6301 ccagctccac cttcaccaaa ttgaacatta cactcttcat ccaacactcc attttcccag
     6361 aaggtttcta ccttttgttt acgaacatcc acattcgatc ctctttctaa aacaataggt
     6421 ttatacccat attgcgcaag aatcaaggct gcaaacatgc ctgccgggcc aaatccaaca
     6481 acgataggtc ttgcagataa agcgtgttct ccactcatta ctggatgata tgtttcatcg
     6541 ggtgttaatg aaacatcttt taattttaaa agtttctttt catttttgac tttacaatct
     6601 acgacatacg aaaacaagac cttctggtgg cgtgcgtcca cactttttct atgtacgtgc
     6661 cactgcaaaa ggtcttcttt acggattcgc agttttttta agatctgtgc ttcaatttca
     6721 ttttcactcg cacatttcac ttgaaataat cgaatcataa gcttacagta atttttttaa
     6781 tatcggatat tgatcctgat aacgagtcac aaaaccagct aaacattgtg cacatgccaa
     6841 agttaagaaa tatacaacta aatgtgcatg tggcatcaaa gctaagaaga ttttggcaaa
     6901 caaaatatgt gaagtataaa tcatcaatga atcgtggcgt aaaattgtaa acattttacg
     6961 atatggaata ttgaccttca ataaataatt cactaagaaa tatacagctg gaactaaaca
     7021 aatatacata cttgttagat cgcgcaaaat attaaaatga cgatatagag ctacttcaca
     7081 aaacaataat acaaaactaa tcaagaaagc aagtaagctt acttttttag gtaagcgacg
     7141 tgttcttgaa agtaacaatc caatcgcaat aaacatagga ccaaaaaaga ttccatttct
     7201 tgcggttgat aatgctttcg taaagaatcc aaataagaaa gaaatataag gtaaactttc
     7261 ccatactggt gtatacacat taattaaata acccacaaca tagaatgcga atgtaataat
     7321 caatgtaaac ttcaagcctt ttttcttata caaataatac acgatcacat ccgccaacat
     7381 taaggctggt aaaaaccaca agtgataata acttccatta aagataaaat ctcgaacgta
     7441 tgtgaataaa tacgatatgc gaaagccatt gtgtgtataa tcaaaaatcg tatatggcaa
     7501 ataaataatt gtccatatca aatagattct aaaaagacga aataatgctt tctttaaacg
     7561 agcatcattc acatcttcat tttccaagtc ataatttcta aagaagaaaa acccgcttgt
     7621 tgtaaagaaa aatggaacgg ccaaacgaca aacagtatga atgaagtacg tattaaatgt
     7681 ttctgatatt tctaaaaatg gaaatgtatg aatacataca accaacaatg ccgaaacata
     7741 acgcgcaata tctatcgatg catactgcct tttcatgagc gcacctttaa tctaggtaca
     7801 cgtgaagaaa ttgaagttaa tgtctcattg ttgatagtct tagccatacg actgatttga
     7861 tctacagtta ccacttcatt tccttgatga ccaacgatgc atacctcatc gccttcttta
     7921 acatcttcta cttctgtaac atcgatcata cattgatcca tacataagtt tccaatcacc
     7981 ttacatcttt gaccatgtac taatacttcg aatccttgat tggataataa acgaggcaat
     8041 ccatccgcat aaccaatgct catagtcgca acttttgttg gtttttcagc tataaaatga
     8101 cgaccataac ttacactttg tcctggataa atccatttca ctaaacttac attggcatac
     8161 atagacatga tgggaataaa atcaggattt gtcttgattt taatatgatc atcactcgta
     8221 acacccatat ataataggcc aggtctacaa tattcatatt caagttctgg ataattcaag
     8281 attccatagc tattttgaag atgacgaata cccggatcaa taccttgcga ttttaataag
     8341 tcaatcactt catcaaacaa ttgaatttgt ttttttgtaa attcagtaca atcttcatct
     8401 aaacaatcac tcactggaaa atgtgaaaag attccttcta cacataagtt ttctaagcga
     8461 tactcttcat aaatatcttc aatatgttta tcttgttctt gatatacaat acccgttcta
     8521 cacatacctg tatctacttt acaatgacaa cgaattttta catcattttc tttggcatag
     8581 gcatttaatt ctctagcata ttcaatggat actaaagatt gaacgatatt ctgttcatga
     8641 agataatgaa aatgcatact tggtgtatat cctaaaatca agatgttatc ttggattcct
     8701 gcatttctaa gatttaatgc ttcatcaata ctggaaactc caaaatagtc cacgccacat
     8761 ttttgtagag ccttagcaca tgcgacatca cctaagccat acgcattggc tttcacaatt
     8821 cccatgattt ttgtgttttt tacaagcttt cgaacctcat ttacattatg acggatcgca
     8881 tcataatcaa tttcagtcca agccctgcta taagcttcta gcatgctcca attgtcctct
     8941 gcatagctag ttcaattagc ttatcaatta aatccgcaaa ttcaacaccg gcttctttca
     9001 tcatagaagg ataacgagaa gtcgctgtta atccaggaat tgtattcaat tcattcaaga
     9061 ttacttcttt atcttctgtc acaaacatat ctacacgtgc cattccgcag caattcatcg
     9121 caatatatgt tttgcgagct aattcttgcg cttctttaaa tgttttttca tcaatacgag
     9181 ccggacaata aatagcggct cctttcattt catattttcc ttcaaaatcg aagaatccac
     9241 ctgtaatttc aatttcatcg actgatcctg taaagatttc ttcatttccc ataacggcac
     9301 aaccaatctc aaagccctta attacttttt cgactaagat cttacctcta ccatcataga
     9361 agaatgcatc tttacatgca ctttcaaatt cttctttgtt gttgacaaaa tgcacaccat
     9421 agctacttcc tgcattacat ggtttaatga tccatggcaa tgcaatctct tcttgaattt
     9481 gttcaaatgt aggattttct cgattggcat ataaacaaat gtagtcagca caaggaattt
     9541 ttgcttcttt acacaaaata tgcatgattt ctttatccat tgacatagca cttgacataa
     9601 catcacaacc tacataatga atattcatca attcaagtaa accttgaatc aatccatcct
     9661 caccattttt accatgtagt accgggaata cacagtctag ttcaatcact ttatttgaac
     9721 catctaatgg catgattccc ccgtttaccc atgcacaaga tacacaagtt tcttcatttt
     9781 cccatgtatc atgttcaata gaatccacat ctccattgta taaatagaaa tgtccgtttt
     9841 tattaatacc aattaaagtc atttcatagt tgtctgcatg aatttgacgt aaaaaggaag
     9901 tgacagaatg taatgaaaca gaatattcac tactttgtcc cccaaatacg acacctaaac
     9961 gtaatttact cattgttttt ccccctaaaa gcatctacta atgtatccat cttcatcgct
    10021 cttgaacctt tgatcataat aatgcaatct ttttctaaca aaggtttaca agcttcatat
    10081 atctcatctt tagtttcata ccatttccca ttaaaaccat cggccgtttc ttttgaaaga
    10141 ggacctgtac aatataattc gtccacacct ttttctttgg cataaacacc cacatcataa
    10201 tgcaactgtt tttcttcact accaagatct aacatatccg ataaaatggc aatatgttta
    10261 ttggccggaa gttccatcaa tgtatcaatg gctgccttag cactttctgg atttgattta
    10321 tatgtatcat ctagaatata tgcatttttt actgaaatta actgcgtacg catctttgtc
    10381 atttctagat ttttcaatcc atttaaaatc gattcttctt ttaaatgaca agcttttgcg
    10441 atatagatac acggcaatgc gttcattgcc tgaaaatcac caaatgcacg caaatgtaca
    10501 ggtgtatcta aaatattaca attaaataca attccttctt tatcatattc aatatcactt
    10561 gtgattgaaa catctccacc ttgaccaaaa gatacgactt ttttattttc tgtatctaaa
    10621 gtacaaagca cttcatggat ttctttactt tcataattat aaaggaacaa tccattttct
    10681 tttgtaccct ctaaaatttc acatttagct tgggcaatct gtaattttcc acccaaattt
    10741 tccatatgag ccgaaccaat tgaagtgatg acagaaatat ccggttgtgc aatggaagtt
    10801 aattgtgtaa tttcaccttt attttccata cccatttcta aaatggccac ttcaatatct
    10861 tcatcaaaat caaatatcgt taaaggaagt ccaatctcac tattgcggtt tccttgtgtc
    10921 ttctgtgttt ttttctcttg tgaaaaaata ctatataaca tatctttaca acttgtttta
    10981 ccatttgaac ccgttacacc aataactaaa ggatgtaact catccagata tgcttttgaa
    11041 agatcctgca tagctttaag tgtatcatct actaaaatta caggaaaatc aggatatggg
    11101 acatggtctt tttgccacaa actaatcaaa gcaccatctt caattgtttg tttggcaaat
    11161 gtatgtccat ccactctttg gccaataata ggtacgtata aaacgtcttt tgtcacttgt
    11221 cgtgaatcaa tcacaacacc at
//
LOCUS       DBMDWL010000061        11059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2039_length_11059_cov_3.212448, whole genome shotgun sequence.
ACCESSION   DBMDWL010000061 DBMDWL010000000
VERSION     DBMDWL010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_11059_cov_3.212448"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(58..1053)
                     /locus_tag="ACZPDN_05600"
     CDS             complement(58..1053)
                     /locus_tag="ACZPDN_05600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1298130.1"
                     /translation="MKQEMSRIPNSNYNLFKKGSKLILSAATFGVVAAGSFQGVNYVV
                     DNYNKQNTTLQSTNVVNTSSSSTSNVSKVAQNCMPSIVSITNVSVSDVQNYFSMYGNN
                     SRSNPFTQQESTSVGSGVIINKQNGEIDILTNYHVIENAKTLTCTLADNTNVKATVKG
                     VDEDRDLAVISIKTSDLSEDTLKQIAIATIGDSDKLQVGEQVVAIGNALGYGQSVTTG
                     IVSATNRSVSTSSDTDKSQSYIQTDAAINPGNSGGALLNMNGELIGINSAKLSDTDVE
                     GMGYAIAISDVKDSINTMLQGKDVSKSSTQSSYYSQYNNNEDDDDDSTSPFDFWN"
     gene            complement(1115..2569)
                     /locus_tag="ACZPDN_05605"
     CDS             complement(1115..2569)
                     /locus_tag="ACZPDN_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR1298131.1"
                     /translation="MGKPTGFLDYKRQENEAIEPLSRITNFNEFHPFLDEETRRKQGA
                     RCMNCGVPFCQSAIKLKNMVTGCPLHNLIPEWNDEIYNGNDKRALSRLLKTNPFPEFT
                     GRVCPALCEKACLNGLDQDPVTIKENELYTIETAYTNNWMKAFIPSVHSDKKVAIIGS
                     GPAGLALAHDLNRRGHAVTVFEKEDRIGGLLMYGIPNMKLEKKVIQRRLDILTEEGIV
                     FKTNVNVGKDITKKELEKEYDAIIFATGSKKARDLNVKNRDCTGIYYAVDYLTKATKH
                     LLDNTEEISAKYKHVVIVGGGDTGNDCVGTSIRQGCASVVQIEMMPKSPDERLESNPW
                     PEWPKICKTDYGQEEAIAVFKKDPRIYETTIKECILDDKKNLKQIKTVKVHFNNGKLE
                     EVKGTEQILDCDLLLIAAGFIGVEDDLKKSFKLETTQRNTIVTQPNSYQVKDQYFTAG
                     DCHRGQSLVVWAIHEGKECAKEVDAYLMGYTNME"
     gene            complement(2586..7043)
                     /gene="gltB"
                     /locus_tag="ACZPDN_05610"
     CDS             complement(2586..7043)
                     /gene="gltB"
                     /locus_tag="ACZPDN_05610"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR1298132.1"
                     /translation="MNFEIEHDACGIGTVVQIDGKQSYDVVDKALHIVEKLEHRAGKD
                     ASGKVGDGVGILLQISDDFFRKTLKKDNIKLPEYYGIGMFFLPRNKLLLNQHKKMFEK
                     FVEQEGCKFITWRKVPCNESILGQKAKDCMPNIYQAFIERPQNISSDVDFDKVLYVIR
                     REFEKSCKETYIASLSSRTIVYKGMFLVSQLRNFYLDLQDKNYKSAIAIVHSRFSTNT
                     TPSWQRAHPNRFIAHNGEINTIRANVNKMLAREESMHSEFLDKNSSKILPIINTSGSD
                     SAMLDNALEFLVMNGMPLEKVVMILIPEPWKHQDMGQKKKDFYHYYATMMEPWDGPAA
                     ILFSDGEKVGAVLDRNGLRPSRYYLTSDNMLILSSEVGVLDIDEKKIVKKSRLEPGKI
                     LLVDTKKKELIEDYDLKNEYASSNPYGEWLDTYLLHLKDLPAPDKKTHIHSQHERDIL
                     YKIFGYTYEDVKDIILPMARVKLEPTSAMGTDIPLAIYSKNHLSLFHYFKQLFAQVTN
                     PPIDSLREEVVTDTTIYIGSDGNLLKDKSDNCTVLEVNNPILTSRDMDKIRQLNQTGF
                     KNETISLLFYRGTSLKEALDNLFIECDKAYRNGANILILSDKGVDEGHMAIPSLLAVS
                     ALEQHLVKTKKKTDVSIIVESGEPRDVHQFATLLGYGATAIYPYLAHECIEEMIQLNM
                     LDKEVNIAIDDYNEAILKGIVKIAAKMGISTLQSYQGAQIFEAIGISKDVIDTYFTNT
                     ISEVEGITLEDIEKDLIYHHDRAYDPLGLTTDTSLDSIGFHKLRKGDGKEDHLYSPET
                     IVKLQRATQTNDYDLFKEYSSELNSNRQKHHLRSQLHFKKTRNSIPLSEVESEYEIVK
                     RFKTGAMSYGSISEEAHTCMAIAMNRLGGKSNSGEGGEKPERLGTEKNSAIKQVASGR
                     FGVTEEYLVSAKEIQIKMAQGAKPGEGGHLPGKKVYPWIAKTRYSTPGVSLISPPPHH
                     DIYSIEDLAQLIYDLKNANPQARISVKLVSEAGVGTIASGVAKAGATVVLISGYDGGT
                     GAASQSSIHHAGLPWELGLAQAHQNLVENGLRSRVLIETDGKLMTGKDVAIACLLGAE
                     EFGFASAPLVTMGCMMMRVCNLDTCPFGVATQNQKLRKRFKGKPEYVMNFMLFIAREL
                     REIMADLGYHTIDEMVGHTDNLEVDEDANMDYSNILMTPHNIHYEPKDTYNFELEKTV
                     DMKTLLPKFEPYFNEKKPHAETISISSTDRTVGTILSNAVTKQFNNTLEDDTYTVHCN
                     GGAGQSFGAFAQKGITLDVHGDANDYFGKGLSGGKLIIQPKEEATFKPSENVIIGNVA
                     LYGATSGEAYIRGMAGERFAVRNSGASAVVEGVGDHGLEYMTGGTVVILGQTGKNLAA
                     GMSGGVAYVYDPNHDLYMRLNKQLVNTYEVTGKADKEVLEELLNKHYTYTDSDVAKKI
                     LSNLDEELSNFKKIVPKDYEKITTLIQELKSKGYHEDEASLMAFEQVHA"
     gene            complement(7061..8644)
                     /gene="asnB"
                     /locus_tag="ACZPDN_05615"
     CDS             complement(7061..8644)
                     /gene="asnB"
                     /locus_tag="ACZPDN_05615"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR1298133.1"
                     /translation="MCTIIGYKSLKISENTIHKALESTYSRGPDDERIQQVGCGYLGF
                     QRLSIMGLSPEGMQPFVRNNNLVVCNAEIYGFRKLKEELEKDGYTFISQSDCEILLPL
                     YEKYGLDMFKKLDAEYACIIYDAKKNDFVAARDPIGIRPLFYGYDKNHNIVFASEAKN
                     LVSIVEQIFPFPPGFYYADGKFTCYLDVTKVDKVITSDLETICTNIHDKLVEGVKKRL
                     DADAPLGFLLSGGLDSSLVCAISQKLLNKPIETYAIGMEEDAIDLKYAKEVADFIGSN
                     HHEIIINKDDVLQAIKPVIKTLATFDITTIRASIGMYLICKAIHEQSNIRVLLTGEIS
                     DELFGYKYTDFAPSADAFQEESVKRVHELYMYDVLRADRCISTNSIEARVPFGDLDFV
                     KYVMSIDPEKKMNTYHKGKYLLRHAFEKDEILPYDILMREKAAFSDAVGHSLSDDIKE
                     YAQSYYTDEEFKEKVKKYKYCTPFTKESLLYREIFESYYPNQASMIKDFWMPNKNWEG
                     CNVNDPSARYLSNYGDSGK"
     gene            complement(8687..10777)
                     /locus_tag="ACZPDN_05620"
     CDS             complement(8687..10777)
                     /locus_tag="ACZPDN_05620"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1298134.1"
                     /translation="MKSNVEEIFGSKVFDESVMREKLAKDTFRELEKTIREGKQLNIK
                     IANAVAHAMKEWALEQGATHYTHWFQPMTGVTAEKHDGFINPDKDGKVIMEFSGKELI
                     KGEPDASSFPNGGLRATFEARGYTAWDPTSYAFIKNGVLCIPTAFCSYSGHALDKKTP
                     LLRSMQAINKQALRIVKLFGNDDVQTIKTTVGPEQEYFLVDQKYYEQRKDLIYTGRTL
                     FGAPCPKGQELEDHYFGTIKSRVQEFMSDLNQELWKLGILAKTEHNEVAPAQHELAPI
                     FTTTNIATDHNQLTMELIQRVAKKHGLVALLHEKPFEGINGSGKHNNFSLSTDTGINL
                     LEPGDTPHENAQFLVFLAAIIKAVDEHQDLLRLSVATAGNDHRLGANEAPPAIISIFL
                     GDELTAVLKAIEEEKPYDGKEKELMKIGATVLPKFPKDSTDRNRTSPFAFTGNKFEFR
                     MPGSNASIAGCNIVLNTVIADEIEKFANILEAAGDFDTTLHALIKDTIKNHKRIIFNG
                     NGYDDSWVKEAESRGLLNLKTTPDALPYFKTKENIELFERQKVLSKEEVQSRYEILMD
                     EYEKQINIEALTMLDIAKKDILPAASKFSKELTDTIANKKAIGIDLTYELETAKKVSE
                     YLTEAYQALITLEKDVEKLHSIDDFEKSAFYVRDFIIQDMAALRKPCDKLEVITSNSD
                     WPFPTYGKLLFGIL"
BASE COUNT         3144 a         2105 c         1894 g         3916 t
ORIGIN      
        1 aaatagattc agactagaat ataatctaaa cagtctagtt ctttttgatt ttatgtttta
       61 gttccaaaag tcaaatggag aagtggagtc atcatcatcg tcttcattat tgttgtactg
      121 tgaataataa gaagattgcg tagatgattt acttacatct tttccttgaa gcattgtgtt
      181 gattgaatct ttgacatcgc tgatcgcaat cgcatagccc ataccttcta catctgtatc
      241 acttaatttg gcagagttga tgccaataag ctcaccattc atatttaata aggcgccacc
      301 tgagtttcct ggattgattg ccgcatcggt ttgaatataa gactgcgatt tgtctgtatc
      361 tgaacttgtt gaaacactac gatttgtcgc cgatacaata cctgttgtaa cactttgacc
      421 ataacctaac gcattaccaa tcgcaacgac ttgttcacca acttgtagtt tatcactatc
      481 tccaattgtc gcaatcgcaa tttgttttaa agtatcttca cttaaatcac ttgttttaat
      541 ggaaattaca gctaagtcac gatcttcatc tacacctttt acagtggctt ttacatttgt
      601 attatccgct aatgtacaag tcaaggtctt ggcattttca ataacgtgat agtttgtcaa
      661 aatatcaatt tcaccattct gtttattgat aataacacca ctaccaacac ttgtactttc
      721 ttgttgggta aatggattgc ttcttgagtt atttccatac atactaaaat aattttgtac
      781 atctgatacg gaaacattgg taatcgatac aatactaggc atacaatttt gagcgacttt
      841 acttacattt gaagttgatg aacttgaagt attgactaca tttgtacttt gaagtgttgt
      901 attttgttta ttgtagttat caaccacata gttgacccct tgaaaacttc cggccgcaac
      961 cacaccaaat gtagcggcac ttaaaattaa ttttgatcct tttttgaata aattatagtt
     1021 agaatttgga attcttgaca tttcttgttt catgtcctta tacctccttg tttacaatac
     1081 aaggatatat taacaagctt aaaacaagtt taaattattc catatttgta taacccatta
     1141 aatatgcatc cacttcttta gcacactctt taccttcatg aattgcccaa acaactaagg
     1201 attgacctct atgacaatca ccagctgtaa agtattggtc tttgacttga taagaatttg
     1261 gttgtgtcac aatcgtattt ctctgtgttg tttccaattt aaaactcttc tttaaatcat
     1321 cttcaacacc aataaaacca gccgcaatca ataataagtc acaatccaag atttgttctg
     1381 tacctttcac ttcttcaagc tttccattgt tgaagtgtac tttgactgtt ttgatctgct
     1441 ttagattttt cttgtcatct aaaatacatt ctttaatcgt tgtttcataa atacgcggat
     1501 cctttttaaa gacagctatt gcttcttctt gaccgtaatc tgttttacag atctttggcc
     1561 attcgggcca tggattactt tccaatcttt catccgggct ttttggcatc atttcaattt
     1621 gaactacact tgcacacccc tgacgaatac ttgttccaac acaatcattt cccgtatcac
     1681 caccaccaac aatcacgaca tgtttatatt tggcagagat ttcttcggta ttatccaaca
     1741 aatgtttcgt tgcctttgta agatagtcca ccgcataata aatgcctgta caatcacgat
     1801 tctttacatt taaatcacgt gcttttttgg atccagtcgc aaagatgatc gcatcatatt
     1861 ctttttccaa ttcctttttg gtgatatctt ttccaacatt gacattggtt ttaaaaacga
     1921 ttccttcttc ggtaagtatg tctaatcttc tttgaatcac tttcttttcc agtttcatat
     1981 tcggaatacc atacatcaat aaaccgccaa tacgatcttc tttttcaaag actgtaacag
     2041 catgtcctct acgattcaaa tcatgagcca aagctaaacc tgcaggtcct gaaccaatga
     2101 tggctacttt tttatcggag tgaacactag gaataaaagc tttcatccaa ttgttagtat
     2161 aggctgtttc aatcgtatac aattcatttt ctttaattgt gactggatct tgatctaaac
     2221 catttaaaca tgccttctca caaagagcag ggcatacacg acctgtaaat tcagggaatg
     2281 gattggtctt taataatctt gataaagcac gtttatcatt gccattatag atttcatcat
     2341 tccattccgg aatcaagttg tgtaaaggac agcctgtcac catgtttttt aatttgatag
     2401 cggactgaca aaagggaacg ccacaattca tgcagcgtgc accttgtttg cgtcttgttt
     2461 cttcatctaa aaaaggatga aattcattaa aatttgtgat tcgactaagt ggctcaatcg
     2521 cttcattttc ttgtcgtttg taatctaaaa atcctgttgg ttttcccata atccttcatc
     2581 ctttcctatg catgtacttg ttcaaaagcc ataagactgg cttcatcttc gtgatagcct
     2641 tttgatttta attcttgaat caatgttgtg atcttttcat aatccttagg aacgatcttt
     2701 ttaaagtttg ataattcttc atctagattt gaaagaatct ttttagctac atctgaatct
     2761 gtatatgtat aatgcttgtt taaaagctct tctaatactt ctttatccgc ttttccagtt
     2821 acttcatatg tattgactaa ctgtttattt aaacgcatat atagatcatg attcggatcg
     2881 tatacatatg caacaccacc agacataccg gcagctaagt ttttaccagt ctgacctaaa
     2941 atgacaacag taccacctgt catatattca agtccatgat ctccaacacc ttctacaact
     3001 gcacttgcac cagaattacg aaccgcaaaa cgctcaccag ccataccacg aatataggct
     3061 tcaccactcg tagcaccata taaggcaaca tttccaataa tgacattttc acttggttta
     3121 aaagtcgctt cttctttagg ctgaataatc aatttaccac cagacaagcc cttaccaaag
     3181 tagtcattgg catcgccatg aacatctaaa gtgattcctt tttgtgcaaa ggcaccaaaa
     3241 gattgtccag ctccaccgtt acaatggact gtatatgtat catcttctaa tgtgttatta
     3301 aattgtttag tgacggcatt ggataaaata gttcctacgg tacgatccgt acttgaaatt
     3361 gagattgttt cagcatgagg ctttttctca ttgaaataag gttcaaactt aggaagtaat
     3421 gtcttcatat ccaccgtttt ttcaagttca aagttatatg tatctttagg ttcataatga
     3481 atgttgtgtg gagtcattaa aatgtttgag taatccatat tcgcatcttc atcaacctca
     3541 agattatctg tatgaccaac catttcatca attgtgtgat agccaaggtc agccataatt
     3601 tctcttaatt cacgggcaat aaacaacatg aaattcatga catattccgg ctttccttta
     3661 aaacgttttc ttaatttttg gttttgtgtt gccacaccaa aaggacaagt atctagatta
     3721 catacacgca tcatcataca tcccatagta acaagtgggg cgctcgcaaa gccaaattct
     3781 tcagcgccta ataaacaggc aatggctaca tcttttccgg tcattaattt tccgtcagtt
     3841 tcaattaaaa cacgacttct taaaccattt tccactaagt tttggtgggc ttgggctaaa
     3901 cctaattccc aaggaagacc ggcatggtga atactacttt gtgaagccgc ccctgtacct
     3961 ccatcataac cagaaattaa gacgacagtt gcaccggctt tggcaacacc tgaggcaatc
     4021 gtaccaacac cagcttcact aactaatttg acactgattc gagcttgtgg gttggcattc
     4081 tttaaatcgt agatcaattg agctaagtct tcaatggagt agatgtcgtg gtgaggagga
     4141 ggggaaatca aagatacgcc tggcgttgaa taacgagttt tcgcaatcca aggatagact
     4201 ttctttcctg gcaagtgtcc accttcacct ggttttgcgc cttgtgccat tttgatttga
     4261 atttctttgg cactgactaa gtattcttcg gtgacaccaa aacgaccact cgccacttgc
     4321 ttaatagcgg agttcttttc tgtacctaaa cgttctggtt tttcaccacc ttcacccgaa
     4381 ttggatttac cacccaaacg gttcatcgca atcgccatgc aagtatgtgc ttcttcagag
     4441 atagaaccat aactcatggc accggtttta aagcgtttta cgatttcata ttcactttcc
     4501 acttccgata atggaattga atttcttgtc tttttaaagt gcaattgact tctcaaatga
     4561 tgtttttgac ggtttgaatt taattcgctg gaatattcct taaataaatc ataatcgttg
     4621 gtttgtgtag ctctttgaag tttcacaatt gtttctggag aatataagtg atcttctttt
     4681 ccatctccct ttcttagttt atggaatcca atggaatcta gacttgtatc ggttgtaagt
     4741 cctaatggat cataggcacg atcatgatgg tagatcaaat ctttttcgat atcttctaaa
     4801 gtaatacctt ccacttcact aatggtgttt gtgaaatagg tatcgatgac atcttttgaa
     4861 attccaatgg cttcaaagat ttgggcacct tgataggatt gaagcgttga aatacccatt
     4921 ttggcggcaa ttttaacaat accctttaag atggcttcgt tataatcatc aatcgcaata
     4981 ttgacttctt tatctaacat atttaattga atcatttctt caatgcattc atgtgccaaa
     5041 tatggataga tggcggttgc accataacct aaaagtgtag cgaattgatg aacatctcta
     5101 ggttcaccac tttctacaat gatagataca tcggttttct ttttcgtttt tactaagtgt
     5161 tgttctaagg cagatacagc taaaagacta ggaatagcca tatgaccttc gtctacacct
     5221 ttatcagaaa gaatcaagat gttcgctcca tttctatagg ctttatcaca ttcaataaat
     5281 aagttgtcta aagcttcttt taaagaagta ccacgataga acaatagaga aatggtttcg
     5341 tttttaaatc ctgtctgatt taattgacga atcttatcca tatcacgaga tgttagaatt
     5401 ggattattga cctctaagac tgtacagttg tcggatttgt cttttaataa gtttccatca
     5461 cttccaatat agatggttgt atctgtaacg acttcttcac gtaaagaatc aattggtgga
     5521 ttggttactt gggcgaagag ttgtttaaaa taatggaata aacttaaatg atttttggaa
     5581 taaattgcca aagggatatc ggtacccatc gcacttgttg gttcaagttt gacgcgggcc
     5641 attggtaaaa taatgtcttt tacatcttca taggtatatc cgaagatctt ataaagaata
     5701 tctctttcat gttgagagtg gatgtgggtt ttcttatcgg gagctggcaa atctttaagg
     5761 tgtaatagat aggtatctag ccattctcca tatggatttg aagaggcata ttcatttttt
     5821 aaatcataat cttcaatcaa ttcttttttc tttgtatcca caagcaaaat tttgcctggt
     5881 tcaagtcttg atttttttac gattttcttt tcatcaatat ctaaaacacc gacttcactt
     5941 gataaaatca acatgttgtc gcttgttaga taataacgac taggtcttag tccgttacga
     6001 tcaagtacag ctccgacttt ttcgccatca ctaaataaaa tggcggctgg accatcccaa
     6061 ggttccatca ttgtggcata gtagtggtag aaatcttttt tcttttggcc catgtcttgg
     6121 tgtttccaag gttctggaat caagatcata acgacttttt ctaatggcat tccattcata
     6181 actaaaaact caagggcatt atcgagcatg gcagaatccg aacctgaagt attgatgata
     6241 ggtaggatct tacttgaatt tttatctaaa aattcagagt gcatactttc ttcacgcgct
     6301 aacattttgt tgacgtttgc acgaatggta ttgatttcac cattgtgcgc aataaaacgg
     6361 tttggatggg ctctttgcca agatggtgtg gtattggtag agaaacgtga gtgtacgatc
     6421 gcaatggcag atttataatt tttgtcttga agatctaggt aaaagtttct taattgggat
     6481 actagaaaca tgcctttata gacaatagta cgactgctta agcttgcgat ataggtttct
     6541 ttacagctct tttcaaattc acgacgaatg acatataaga ctttgtcaaa atctacatca
     6601 ctcgaaatat tttgaggtct ttcaataaaa gcttgataga tatttggcat gcagtccttg
     6661 gccttttgac ctaagataga ttcgttgcaa ggaacttttc gccatgtgat aaatttgcag
     6721 ccttcttgtt ctacaaattt ttcaaacatc tttttgtgtt gatttaataa aagtttattt
     6781 cttggtaaga aaaacatacc gattccatag tattctggta atttgatgtt gtctttcttt
     6841 aatgtttttc taaaaaagtc atcactaatt tgaagtaaga ttccaacacc atcccccact
     6901 ttaccactcg catctttacc ggctctatgt tctaattttt ctacaatgtg taatgcttta
     6961 tcgacaacgt catagctttg tttgccatcg atttggacta cggtaccaat accgcaggca
     7021 tcatgttcaa tttcaaaatt catagtttac acaatccttc ctattttccg ctgtctccat
     7081 agtttgacaa atatcgagcg cttgggtcat tgacgttaca tccttcccag ttcttatttg
     7141 gcatccagaa atctttgatc atactcgctt ggtttggata gtaggattca aaaatctctc
     7201 gatataatag ggattccttt gtgaatggag tacagtattt gtattttttt actttttctt
     7261 taaattcttc gtctgtgtaa tagctttggg catattcttt gatgtcatcg cttaaagagt
     7321 gtcctaccgc atccgagaag gcggcttttt cacgcatcaa aatgtcataa ggaagaattt
     7381 catctttttc aaatgcatga cgaagtagat attttccttt atgatatgta ttcattttct
     7441 tttcaggatc tatggacatg acgtatttga caaagtctaa atcgccaaaa ggaacacgag
     7501 cctcgatgga gtttgtggaa atacaacgat cggctcttaa tacatcatac atgtaaagtt
     7561 catgtactcg ttttacactt tcttcttgaa atgcatctgc acttggggca aagtcggtat
     7621 atttataacc aaacaattca tcactgattt ctccggtaag taatacgcga atgttacttt
     7681 gttcatgaat ggctttacaa attaaataca ttccaatgct ggcgcgaatg gttgtgatgt
     7741 caaaggttgc caaagtttta atgacgggtt taatagcctg taaaacatca tctttattaa
     7801 ttataatttc atggtggttg ctaccaataa agtcagcgac ttctttggcg tattttaaat
     7861 caatcgcatc ttcttccatt ccaatcgcat aggtttcgat gggtttgttg agtaattttt
     7921 ggctgatggc acatactaga cttgaatcca atcctccact taataagaat cctagtggag
     7981 catcggcatc taatcttttc ttaacacctt ctactaattt atcgtggatg tttgtacaga
     8041 tggtttcaag atctgaagtg attactttat ccactttagt aacatctaag taacaagtga
     8101 attttccatc ggcataatag aatcccggag ggaagggaaa gatttgttca acaattgaaa
     8161 caagattttt agcttcggaa gcgaatacga tattatggtt tttatcatag ccatagaaca
     8221 agggacgaat accaatgggg tcacgggcag caacaaagtc atttttcttg gcatcataaa
     8281 taatgcatgc gtattcggca tctagctttt taaacatgtc taaaccatat ttttcataga
     8341 gtggtaaaag aatttcacaa tcactttgcg aaataaatgt atagccatct ttttcgagtt
     8401 cttcttttaa ctttcgaaag ccatagattt cagcattaca tacgacaagg ttgttatttc
     8461 taacaaatgg ctgcatgcct tctggactga gtcccataat ggatagtctt tgaaaaccta
     8521 aatagccaca tcctacttgt tggattcttt catcatcagg tcctctagaa taggtgcttt
     8581 ccaatgcttt atgaattgtg ttttcgctta tttttaatga tttatatcct ataattgtac
     8641 acataaataa tatctcctta gaacggaaaa gccggcaatt gccggtttat agaattccga
     8701 ataataattt tccataggtt gggaatggcc aatcactatt agaagtaatg acttcaagct
     8761 tatcacatgg ttttcttaag gccgccatgt cttggataat gaagtctctt acatagaagg
     8821 ctgatttttc aaaatcatcg attgaatgta gtttttcaac atctttttct aatgtgatca
     8881 aagcttgata agcttcggtt aaatattcac tgaccttttt agctgtttca agctcatatg
     8941 ttaagtcaat gccaatggct tttttgtttg cgatggtatc tgtcaattct ttagagaact
     9001 tacttgcggc tggtaagatg tcttttttgg cgatgtctaa catagtcaat gcttcaatgt
     9061 tgatttgttt ttcatattca tccattaaga tttcataacg agattggact tcttcttttg
     9121 ataagacttt ctgtctttca aataattcaa tgttttcttt tgttttgaaa tatggcaaag
     9181 catcaggcgt tgtcttaaga tttaatagac ctctagattc agcttcttta acccaagaat
     9241 catcgtagcc attgccattg aagataatgc gtttgtggtt cttgattgta tctttgatca
     9301 atgcatgtaa tgtcgtgtca aaatcacctg cagcttctaa gatattcgca aatttttcga
     9361 tttcatccgc aatcactgta tttaatacaa tgttgcatcc ggcaatactt gcgtttgatc
     9421 ctggcattct aaattcgaat ttatttcctg taaatgcgaa tggagaagta cggttacgat
     9481 cggtagaatc tttagggaat ttaggaagaa cagtagcacc aatcttcatt aattcttttt
     9541 cttttccatc atatggtttc tcttcttcaa tggcctttaa tacagcggtt aattcatcac
     9601 ctaagaagat ggagataata gctggaggag cttcatttgc acctaatcgg tgatcgtttc
     9661 ctgcagtagc tacgcttaat cgtaataagt cttggtgttc atcaacggct ttgataatgg
     9721 cagctaagaa aactaagaat tgcgcgtttt catgaggtgt atcgcctggt tctagtaggt
     9781 tgattcctgt atctgtagat aaagagaagt tattgtgttt accacttccg ttgatgcctt
     9841 caaatggttt ttcatgaagt agggcaacca atccatgttt tttggcaact ctttgaatca
     9901 attccatcgt caattggtta tggtctgttg cgatatttgt cgttgtaaaa attggggcta
     9961 attcatgttg agcaggggct acttcgttat gttccgtttt agctaagatg ccaagtttcc
    10021 acaattcttg atttaaatcc gacatgaatt cttgtacacg cgatttaata gttccaaaat
    10081 agtgatcttc caattcttgt cctttagggc atggtgcacc aaatagtgta cgtcctgtat
    10141 agatcaagtc ttttctttgt tcataatatt tttggtccac taagaaatat tcttgttcag
    10201 gtcctacggt tgttttaatg gtctgaacat cgtcattgcc aaatagtttc acaatacgta
    10261 aggcttgttt attaatggct tgcatacttc taagtagtgg ggttttcttg tctagggcat
    10321 gtcctgagta agaacagaat gcggttggaa tacatagtac gccattttta ataaaggcat
    10381 aacttgttgg atcccaagct gtatagcctc tagcttcaaa tgtggctctt agtccaccat
    10441 ttggaaaact tgaggcgtcg ggttctcctt tgattaattc tttaccacta aattccataa
    10501 tgactttgcc atctttatcg ggattaataa agccatcatg cttttcagca gtaactcctg
    10561 tcattggttg gaaccagtgt gtatagtgtg ttgcaccttg ttctaaagcc cattctttca
    10621 tggcgtgggc taccgcattt gcgattttga tatttaactg tttaccttcg cgaatggttt
    10681 tttctaattc gcggaaagta tcttttgcga gtttttctcg cataacagat tcatcgaaaa
    10741 ctttgcttcc aaaaatttct tctacgtttg atttcataat ggctctcctt tacataaaaa
    10801 aagggcaagg acacgaaaaa catgagggat aaacctctgc ttttttttgg cgtccttgcc
    10861 atgtatgcat tcattataaa cagatatttt gtattgtcaa tgaaaaaatg taaaaagatt
    10921 tgaaaacatt atgaaaatag tgtattaatt attcgaggtg aaaattatga cttatgaaga
    10981 tgtattgagt ttttgcgaag aacaagacgt tcgttttatt cgtctttctt attttgatat
    11041 ttatggtaga cagaaaaat
//
LOCUS       DBMDWL010000062        10852 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2093_length_10852_cov_2.261557, whole genome shotgun sequence.
ACCESSION   DBMDWL010000062 DBMDWL010000000
VERSION     DBMDWL010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2093_length_10852_cov_2.261557"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(166..>358)
                     /locus_tag="ACZPDN_05625"
     CDS             complement(166..>358)
                     /locus_tag="ACZPDN_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298135.1"
                     /translation="CLRSNDLFTRTSETQLLLLVDCQTEENAHLIIQRISNKFYSIFR
                     KKNYRMNYSVHKAELDRNR"
     assembly_gap    359..368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<369..1359)
                     /locus_tag="ACZPDN_05630"
     CDS             complement(<369..1359)
                     /locus_tag="ACZPDN_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015649.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR1298136.1"
                     /translation="MSETYYVKTFGGLEITNDNVTVNIVELFGKQLVNLLQVLLFHSE
                     KPVQKDELIDILWPESKNPSSALKFSIFRLRSELNEIDFFKDKEVIVTTRKGYILNPN
                     LDWNIDFVELQKAYNQINEGAELLDEKEFKIARKIFRLYQGRFYASPSQLHWILQKQE
                     VFRQMYVKTMMRTSCYLYTQKRYDEMMLMNYQAVLIEPFNEGLHYYYMKGLVATRNYR
                     EALKYYDELNDIFLSELGTGLSKRFKQLYDIIIADHAKEENKDMETIIRELSNRDQQN
                     QGFFCSYEIFKYFYELLLKMSVCNEQNYYLIMLQFSDGTLDYEKVGVDFDRVKR"
     gene            complement(1466..1945)
                     /locus_tag="ACZPDN_05635"
     CDS             complement(1466..1945)
                     /locus_tag="ACZPDN_05635"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR1298137.1"
                     /translation="MKLTLIAAIGKNRELGKNNDMIWHLPQDLKFFRQETSGHTIVMG
                     RKTFESLPGKLPKRHHVVISRTCKDLGEDIEMCESVEEFMKRYKDVDEEIYCIGGGMI
                     YSEMLKYASRLVLTQIDADADAEVYFPEFDESLYNKKILSNIEENGVFYQHVEYTLK"
     gene            complement(1953..2816)
                     /gene="thyA"
                     /locus_tag="ACZPDN_05640"
     CDS             complement(1953..2816)
                     /gene="thyA"
                     /locus_tag="ACZPDN_05640"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR1298138.1"
                     /translation="MSYFDDVYCGLCKDILENGVQVHNRTGIDTIKIPSAHFHLDVSK
                     EFPILTTKQLFIRQAVTEMLWIYQAQSNDVRWLQERNVHIWDKWEINEDGDWIDENTG
                     NVLKHFDPSFAHTIGTAYGYIVKKYDLMNKLLNSLKNDKEDRRRVISLWQDSELDTAV
                     LPSCVWSSEWDVTDGKLNIWVHQRSCDVPLGLPFNVTQYAVLLMMVAQVTGLKPGTID
                     WSIKDAHIYVNQIDGIKEQLNRYETKGSLPAPELWLNPDITNFYDFDPTKDVKLNGYE
                     HMGKISFPLTQ"
     gene            complement(2803..3636)
                     /locus_tag="ACZPDN_05645"
     CDS             complement(2803..3636)
                     /locus_tag="ACZPDN_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1298139.1"
                     /translation="MKPITLIATDLDGTFLNSKKKVTSLNKEAVSKLKQYGILFGIAS
                     GRPVETVRAMLKEWQIEDSVSFIMGMNGGVLYDLRQRSKEEYHVLDGEIVLDIIHFFE
                     DLDVDFWILEGATRYTNRTSVETQEHARIFGETEIEIDLEPYLKNNTCNKLIIHCDKE
                     YMPIVLERAKQYKNEACVGFLTADNLLEYVDPRINKGYGIEKVAKHFGVKLENCVAFG
                     DEQNDMEMLQKVGIGVCMKNGSKQVKDCGAFVSAYTNDESAVAHFIEENILKEDMDVI
                     L"
     gene            complement(3640..5019)
                     /locus_tag="ACZPDN_05650"
     CDS             complement(3640..5019)
                     /locus_tag="ACZPDN_05650"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1298140.1"
                     /translation="MSEVVWNKYSKEEFENVMKFSDGYIDFLSDSKTERACVKNAIAL
                     AKSYGYRDIKEVIENKEILKAQDKVYVNMMNKSIALMHIGSNPLEKGMNILGAHIDSP
                     RLDLKQNPLYEDGNLAYLDTHYYGGVKKYQWVTLPLAIHGVVCLKDGTTVDIAIGDKE
                     SDPVFVISDLLIHLSADQLQKKANEVIAGEDLNVTVGSIPLENEEKDAVKANVAKILK
                     STYGFEEEDFVSAELEVVPAGRARSCGFDSSMVLGYGHDDRICAYTSLVAQLEAENVK
                     RTAVCLLVDKEEVGSQGATGMHSMFFENFVAEVMECMGDYSALSVKRAMMNSTMLSSD
                     VSAAHDPIYASASSPRNQAEFGKGIVFNKYTGARGKSGCNDANAEYIALIRRIMDDHN
                     VAWQTSELGKVDQGGGGTIAYILANYGMQVIDCGVALHNMHAPFELASKADIYEAKKG
                     YAAFLTYEG"
     gene            complement(5016..5792)
                     /gene="murI"
                     /locus_tag="ACZPDN_05655"
     CDS             complement(5016..5792)
                     /gene="murI"
                     /locus_tag="ACZPDN_05655"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR1298141.1"
                     /translation="MNSKNPIGVFDSGLGGISVLHTMHSLLPNENLIYLGDSKYNPYG
                     TKTKEEITKRCIAICDEFIKQDVKAIVIACNTATSACVKLLRDKYDVDIIGMEPALKV
                     ACDRGSNQRIAVWATELTLSEKKFANLMDRFKDEHHIVKVPCPKLVRMVEDDKLDDTN
                     LVESVLKEYLAACDYKNLDSIVLGCTHFPFYTKYLERLCPNIHIIEGSVGTTLHTKDL
                     LEQKGLLNRSDEIGSITWMNTMESKIELSKRLFKMLEVEA"
     gene            complement(5872..6252)
                     /locus_tag="ACZPDN_05660"
     CDS             complement(5872..6252)
                     /locus_tag="ACZPDN_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298142.1"
                     /translation="MKISNYFAKEKMDDATFIQTFIEKLNDLTDEDICWANSDLLEDN
                     ENVQSLLMEMVLSFYATDLVDMKDYEDYLVNKEFEAMSFKELQASLIYLFHNDRVHPD
                     SLIHDGIGKKELLNILKEMKNQYS"
     gene            complement(6615..7121)
                     /locus_tag="ACZPDN_05665"
     CDS             complement(6615..7121)
                     /locus_tag="ACZPDN_05665"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1298143.1"
                     /translation="MSEFIDLYDQNRKLLNREVDRTTYLFQPGEFMMYVLAILENSQG
                     EFLVTQRAFNKKWAAGNWEMPGGGAKSKESSLDAIKREVKEETGLDVKNGGVVYSYFN
                     EDNDRHDNYFVDIYHFKFDFDLSDVQLNTCESINCKCVTLDELISMNQEQSFLHYERI
                     MKALQQEK"
     gene            complement(7114..7446)
                     /gene="hisI"
                     /locus_tag="ACZPDN_05670"
     CDS             complement(7114..7446)
                     /gene="hisI"
                     /locus_tag="ACZPDN_05670"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR1298144.1"
                     /translation="MMQLDFKKQNGLIPCIVQDVDTKKVLMLAYMNEESYRQTVETKL
                     ATYYSRSRQSLWVKGETSGHYQHVKKIRIDCDQDTILLKVEQDGAACHTGHYSCFYRD
                     ENGDCVDE"
     gene            complement(7443..8198)
                     /gene="hisF"
                     /locus_tag="ACZPDN_05675"
     CDS             complement(7443..8198)
                     /gene="hisF"
                     /locus_tag="ACZPDN_05675"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1298145.1"
                     /translation="MLTKRIIPCLDVKDGQVVKGIHFLGLKEVGDPVSLAKKYYEDGA
                     DELVFLDISATIEKRKTMVDVVRRVAKEIFIPFTVGGGISTIEDVRAMLMAGADKVSL
                     NSAAIRNPSLIEEASKRFGNQCIVLAVDAKKRGDNTGWNVYVEGGRKDTGIDCMEWIL
                     KGVSLGAGEILLTSMDADGTKDGFDLDLYRAVSKIVNVPVIASGGCGKLEDFKSALEV
                     SDAALAASIFHYEESSVLNVKKYLKEEGVAVRL"
     gene            complement(8192..8902)
                     /gene="hisA"
                     /locus_tag="ACZPDN_05680"
     CDS             complement(8192..8902)
                     /gene="hisA"
                     /locus_tag="ACZPDN_05680"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR1298146.1"
                     /translation="MIVLPAIDILDGKPVRLYQGDYGQSKCVAEDVLACAKKFEKEKA
                     NYLHVVDLNGAKEGSRVNADLICEVVKTCQLPVEVGGGIRTMEAIDFYIENGVSRVIL
                     GTSAIQDDCLLKDAIKKYGSKIAVGLDCKDGYVCTSGWLEKSKEYYLDFAKRMEKIGV
                     KTLICTDISKDGTLQGPNFEMLEALKNHVSCNLIASGGVRDLSHIQKCKDLGLYGVIA
                     GKAIYEKTLDLKEALSIC"
     gene            complement(8903..9502)
                     /gene="hisH"
                     /locus_tag="ACZPDN_05685"
     CDS             complement(8903..9502)
                     /gene="hisH"
                     /locus_tag="ACZPDN_05685"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1298147.1"
                     /translation="MIGIIDYGMSNLHSVKNACDYLNLDSVVSKDVSVLSKCDKLILP
                     GVGAFQDCMNTLHSMGLYDFVVQQVKKDVPLLGICLGMQALFESSEENGYCLGFGFIK
                     GHVCHMEDTSVKIPQIGWNVLESSQAQNIFKENTYVYYVHSYYAKDMEDQDLIGYSMY
                     GNMKIPGLVMHGHIMGCQFHPEKSGKDGLKILEYFGKEF"
     gene            complement(9499..10080)
                     /gene="hisB"
                     /locus_tag="ACZPDN_05690"
     CDS             complement(9499..10080)
                     /gene="hisB"
                     /locus_tag="ACZPDN_05690"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR1298148.1"
                     /translation="MRTAEKSRDTKETKIYEKINLDGNGNSEIDTGIGFFDHMLTLFA
                     FHSNFDLVVKADGDLEVCDHHTIEDCGILLGKLFLEALGDKKGIARYGSMNLPMDEVL
                     CNVTLDISGRPYLVFNCDFNRENIGTMSCEMVEEFFRAFSVAAGVTLHINVFYGKNDH
                     HKVEAIFKAFGRALKQAVKIESDKIPSSKGVLE"
     gene            complement(10067..10609)
                     /locus_tag="ACZPDN_05695"
                     /pseudo
     CDS             complement(10067..10609)
                     /locus_tag="ACZPDN_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            10593..>10852
                     /locus_tag="ACZPDN_05700"
     CDS             10593..>10852
                     /locus_tag="ACZPDN_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298149.1"
                     /translation="MADGSIQILACAKENSGDCIRRGTVHNVDKTSECLKNIKQRLQD
                     VVKKNISKVYVCIGGQSLHSVLNTVSRDFETETKITQEIVDA"
BASE COUNT         3493 a         2352 c         1273 g         3724 t
ORIGIN      
        1 gatccatagt tgtatatgtt gccttattta aactatcgtc tgcatgattg ataaaaagaa
       61 aggccatcaa taaaattcca agcaccatca aaatagagaa gatcaatagt ttataaattc
      121 ttttccttaa tacacttgca tccatataca atctacatct ttctattatc tgtttctatc
      181 tagttctgct ttatgaactg agtaattcat acgatagttt ttctttctaa aaatactata
      241 aaatttattc gaaatacgct gtataatcaa atgcgcattc tcttctgttt gacaatccac
      301 tagaagtaat aattgcgttt cacttgtacg tgtaaacaag tcgttacttc ttaagcatnn
      361 nnnnnnnnat ctttttacgc gatcaaaatc cacacctact ttttcataat ctaaagttcc
      421 gtctgagaat tgtaacataa tcaaataata attttgttca ttacaaactg acattttcaa
      481 caacaattca tagaaatatt taaaaatttc atacgaacag aaaaagccct gattttgctg
      541 atcacgattt gaaagttccc ttataattgt ttccatatct ttattttctt ctttagcatg
      601 atcagcaata ataatgtcgt acaattgttt aaaacgtttt gataaacccg ttccaagttc
      661 cgacaagaaa atatcattta attcatcata atacttcaat gcctctcgat aatttcgcgt
      721 tgcgaccaaa cccttcatat aatagtaatg taagccttca ttaaaaggtt caatcaatac
      781 agcttgataa ttcataagca tcatttcatc gtatcgcttt tgcgtataca aataacaaga
      841 tgtacgcatc attgttttaa catacatttg tctaaatact tcttgttttt gtagaatcca
      901 atgaagctgg cttggtgaag cataaaaacg tccttgatat aatctaaata tttttctagc
      961 aatcttaaac tctttttcat ccaacaattc agcgccctca ttgatttgat tataagcctt
     1021 ttgtaattct acaaaatcaa tattccaatc taaatttgga tttaagatat agccctttct
     1081 tgtcgtaaca ataacttctt tatccttaaa aaaatcaatt tcatttaatt cggaacgcaa
     1141 tctaaaaata gagaatttta atgcactaga cggattttta ctttccggcc ataaaatatc
     1201 gatcaattca tctttttgaa ctggcttctc actatgaaac aataacactt gcaacaaatt
     1261 caccaattgt ttgccaaata attccacaat attgaccgtc acattatcgt ttgtgatttc
     1321 caatccccca aaggttttta cataataagt ttcactcatg atgatcctcc tttcaaaaaa
     1381 ttatatagta tctaacctag ttttatttta caataaattt cacaaacaaa aaagcagaga
     1441 attagaaaat attctctgca aatcattatt ttaatgtata ttcaacgtgt tgatagaaaa
     1501 caccattctc ttcaatattt gaaagaatct ttttattgta taaagattca tcaaattcag
     1561 gaaaatacac ttcggcatcg gcatcggcat caatttgcgt taacacaagt cttgatgcat
     1621 attttagcat ttcactatag atcatgccac cgccaataca atagatctcc tcatccacat
     1681 ctttataacg cttcatgaat tcttcaacac tttcacacat ttctatatct tcaccaagat
     1741 ctttacatgt tcttgaaata acaacatgat gacgcttagg caacttacca ggaagactct
     1801 caaacgtttt tcttcccatc acaatcgtgt gacctgaagt ttcttgtcta aaaaacttta
     1861 agtcttgcgg caaatgccaa atcatatcat tattctttcc gagttcacga tttttaccaa
     1921 tcgcagcaat caacgttaat ttcataaacc acctattgtg ttaaaggaaa actgattttt
     1981 cccatgtgct cgtatccatt caatttaaca tcttttgtag gatcaaaatc atagaaattt
     2041 gtaatatctg gattcaacca taattctggc gctggcaaag aaccttttgt ttcataacga
     2101 ttcaactgtt ctttgattcc atcaatctgg tttacataaa tatgcgcatc cttgatagac
     2161 caatcgatcg tacctggttt taatcctgta acctgagcca ccatcattaa taaaactgca
     2221 tattgagtaa cattaaaagg caatcctaaa ggaacatcac aacttctttg atgaacccaa
     2281 atattcaact tgccatctgt cacatcccat tctgagctcc atacacaact tggaagtaca
     2341 gctgtatcta gttcactatc ttgccataaa gaaatgacac gtctacgatc ttctttatca
     2401 ttttttaacg aatttaaaag cttattcatt aaatcatatt tttttacaat atacccataa
     2461 gccgttccaa tcgtatgggc aaaacttgga tcaaaatgtt ttaatacatt ccctgtattt
     2521 tcatcaatcc aatctccatc ctcattaatt tcccatttat cccaaatatg aacatttctt
     2581 tcttgaagcc aacgaacatc attactttgt gcttgataga tccataacat ttctgtaaca
     2641 gcctgacgaa taaacaattg tttcgttgtt aaaattggaa attctttaga tacatccaaa
     2701 tggaaatgtg cactcggaat tttaatcgtg tcaattccag tacgattatg tacttgaact
     2761 ccattctcta aaatatcttt acataatccg caataaacat catcaaagta tgacatccat
     2821 gtcctccttt aaaatatttt cttcgataaa atgagccact gcactttcat cgtttgtata
     2881 cgcactcaca aatgcaccac aatctttaac ctgtttagag ccatttttca tacagactcc
     2941 gataccaacc ttttgaagca tctccatatc attttgttca tctccaaagg caacacagtt
     3001 ttcaagtttg acaccaaagt gcttggcaac cttttcaatt ccatatccct tattgattct
     3061 aggatctaca tactctaata agttatcggc tgttaaaaaa ccaacacaag cctcattctt
     3121 atattgttta gcacgctcta atacaattgg catatattct ttatcacaat gaataatcaa
     3181 cttattacaa gtattattct ttagatatgg ttcaagatca atttcaatct ctgtttcacc
     3241 aaagattcga gcatgttctt gtgtttctac actcgtacga tttgtatatc gagtagctcc
     3301 ttctaaaatc cagaaatcta catctagatc ctcaaaaaaa tgaatgatat ctaatacaat
     3361 ttctccatct agcacatgat actcttcttt tgaacgttgt cttaaatcat ataagacacc
     3421 accattcatc cccataataa agcttacact atcttcaatt tgccattctt ttaacatggc
     3481 acgtactgtt tcaacaggac gaccagaagc aatcccaaac aaaatgccgt attgtttcaa
     3541 ttttgaaact gcttctttat ttagagatgt aactttcttc tttgaattta agaaagttcc
     3601 atccaaatct gtcgcaatca aagttattgg tttcatatat tatccttcgt aagttaagaa
     3661 tgctgcatat cctttcttgg cttcataaat atccgcttta ctagccaatt caaatggagc
     3721 atgcatatta tgtaatgcta caccacaatc aataacttgc attccatagt ttgccaaaat
     3781 atatgcgatt gttccaccgc caccttggtc tacttttcca agttcacttg tttgccatgc
     3841 cacattgtga tcatccatga tacgacgaat caatgcaata tattcagcgt tcgcatcgtt
     3901 acatccactc ttaccacgag cacctgtata tttattaaat acaataccct ttccaaattc
     3961 tgcttgattt cttggactgg atgccgatgc ataaattgga tcatgtgcag cacttacatc
     4021 acttgaaagc atagttgaat tcatcattgc acgttttaca cttaaagcgg aataatcacc
     4081 catacactcc ataacttcag caacaaagtt ttcaaagaac atagaatgca tacctgtagc
     4141 accttgagaa ccaacttctt ctttatccac taataaacaa acagctgtac gttttacatt
     4201 ttcagcttcc aattgagcca ctaaactagt ataagcacaa atacgatcat catgaccata
     4261 acctaatacc atacttgaat caaagccaca gcttcttgca cgtcctgcag gaaccacttc
     4321 taattcagct gaaacaaaat cttcttcttc aaaaccataa gttgatttta aaatcttagc
     4381 tacattagcc ttaaccgcat ctttttcttc attttccaaa ggaatggatc caactgttac
     4441 atttaaatct tcccccgcaa tcacttcatt tgcctttttt tgtaattgat cagcacttaa
     4501 gtgaatcaat aaatcgctaa tcacaaatac aggatcactt tctttatctc caatcgcaat
     4561 atcaacagtt gttccatctt ttaaacaaac aacaccatga atagccaaag gcaaagtaac
     4621 ccattgatat tttttgactc caccataata gtgagtatct aaataagcta aatttccatc
     4681 ttcatataat ggattttgtt ttaaatctaa acgaggacta tcgatatgag cacctaaaat
     4741 attcattcct ttttctaaag gatttgatcc aatgtgcatc aacgcaatcg atttattcat
     4801 catatttaca tacactttgt cttgagcctt taaaatttct ttattttcga taacctcttt
     4861 gatatcacga taaccatatg actttgctaa tgcgatggca ttttttacgc aagctctttc
     4921 cgttttagaa tctgacaaaa aatcaatgta tccatcactg aatttcataa cattttcaaa
     4981 ttcttctttt gaatatttat tccaaactac ttcactcatg cttctacctc caacatttta
     5041 aataagcgtt ttgataattc tatttttgat tccattgtat tcatccaagt aatacttcct
     5101 atttcatcac tcctatttaa caagcctttt tgttccagta aatccttagt atgcaatgtt
     5161 gttcctacac taccttcaat aatatgaata tttggacata gtctttctaa atattttgtg
     5221 taaaaaggaa aatgagtaca tccaagtaca atactatcta gatttttata gtcacaagcg
     5281 gccaaatatt cttttaaaac agattctaca aggtttgtat catccaattt atcatcttcg
     5341 accattctta ctaactttgg acaaggtact ttcacaatat gatgctcatc tttgaatcga
     5401 tccatcaaat tcgcaaactt cttttcagat aaagtaagtt cggttgccca taccgcaatc
     5461 ctttgattac tccctcgatc acacgccact tttaaagctg gctccattcc aatgatatct
     5521 acatcatatt tgtctcttag aagcttaaca caagcacttg ttgccgtatt acatgcgatt
     5581 acgattgcct ttacatcttg ttttataaat tcatcgcaaa tcgcaataca tcgcttagtg
     5641 atttcttctt ttgtctttgt tccataagga ttgtacttac tatctcctaa gtaaattaaa
     5701 ttttcattag gaagtaagct atgcatcgtg tgtaaaacac taattccacc taatcctgaa
     5761 tcaaaaacac ctatcggatt tttcgaattc atacaaaacg ctcctttttt catcttttca
     5821 attatacaac gtataaagaa aacatccaag tcctcaaact tggatgcatg tttagctata
     5881 ttgattcttc atttctttta agatatttaa taattccttt ttaccaatgc catcatgaat
     5941 caaactatcc ggatgcactc tgtcattatg aaataaatag atcagtgagg cctgaagctc
     6001 tttaaatgac atagcttcaa attctttatt caccaagtaa tcttcataat ctttcatatc
     6061 aactaaatct gtcgcataaa aagaaagaac catctccatc aataaagatt gaacattctc
     6121 attatcttca agtaaatctg aatttgccca acaaatatct tcatctgtta aatcatttaa
     6181 tttttctata aatgtctgaa taaatgttgc atcatccatt ttttctttcg caaaataatt
     6241 tgaaattttc atattctcct ccaaaaaaaa gaggtttaag gctttcatca cttccttaaa
     6301 ccgctttccg taatatttat atcctcatat aaatattaca tgaattttgt ttgtatttaa
     6361 tattattatg ttcttgtaaa caaacaaaat tctgtataga taaaaccacc acattctatc
     6421 tatactctct aatccacttt tccttcgcag agtttaccac gctctgcatg tccctttggc
     6481 agctcatcac actgccaagt aaataatagc acatataaaa agggtaagca agcttaccct
     6541 tctgtcagtc tatgacctat aaaggaagta gttggtagtg tttcagtcat agaacttaca
     6601 cctatatttt agtcttattt ttcttgttgt aaagccttca taattctttc ataatgcaaa
     6661 aaagattgtt cttgattcat tgaaatcaat tcatctaaag taacacactt acaatttata
     6721 ctttcgcatg tatttaattg cacatcactt aaatcaaaat caaacttaaa atgataaata
     6781 tccacaaaat aattatcatg tctatcatta tcttcattaa aataagaata gacaacccca
     6841 ccatttttta catcaagacc tgtttcttcc ttgacttcac gcttgatcgc atccaaactt
     6901 gattctttac ttttagcgcc tccaccaggc atttcccaat ttccagcagc ccatttttta
     6961 ttaaaagctc tttgcgtaac tagaaactca ccttgagaat tctccaaaat agccaaaaca
     7021 tacatcataa attctccagg ctgaaataaa taggtagtac gatccacttc tcgatttaac
     7081 aacttacgat tttgatcata caaatcaata aattcactca tctacacaat ctccattttc
     7141 atctctataa aagcaagaat aatgtcctgt atgacaagct gctccatctt gttctacttt
     7201 taataatatc gtatcttgat cacaatcaat gcgaattttt ttcacatgtt gataatggcc
     7261 actcgtttct cctttgaccc ataaactttg ccttgaccga gaataataag tggcaagttt
     7321 tgtttcaaca gtttgtctat aactttcctc attcatatat gcgagcataa gaactttttt
     7381 agtatccaca tcctgaacga tacaaggaat caagccattt tgtttcttaa aatccaactg
     7441 catcataacc ttactgcgac tccttcttct tttaaatact tctttacatt taaaacacta
     7501 gattcttcat aatgaaaaat acttgctgca agtgcagcat cactcacttc taatgcagat
     7561 ttaaaatctt ctaatttacc acatccacca gaagcaatca caggtacatt tacaatttta
     7621 gaaacagctc tatataaatc taaatcaaag ccatcttttg ttccatccgc atccatactt
     7681 gtcaacaaga tttctccggc tcctaaagat acccctttta agatccattc catacaatcg
     7741 attcctgtat cttttcttcc accttctaca tatacattcc aacctgtatt atcgcctctt
     7801 tttttagcat ccaccgccaa tacgatacat tgattgccaa atcgtttact tgcctcttca
     7861 attaaagaag gatttcgaat agctgcacta tttaaactta ccttatcggc tccggccatc
     7921 aacatagcac gcacatcttc aattgttgaa atacctccac ctactgtaaa aggaataaat
     7981 atttcttttg cgacacgcct aacaacatcg accattgttt ttcttttttc tatagttgcg
     8041 ctaatatcta aaaacaccaa ttcatccgca ccgtcttcat aatatttttt tgccagagaa
     8101 actggatctc caacttcttt taggcccaaa aaatgaatgc ctttcaccac ctggccatct
     8161 ttcacatcta aacaaggtat gattcttttt gtcaacatat gcttaacgcc tctttcaaat
     8221 ctaatgtttt ctcatagatg gccttcccgg caataactcc atataaccct aaatctttac
     8281 atttttgaat gtgtgacaaa tcacgtacac caccagatgc gattaagtta cacgatacat
     8341 gatttttaag tgcttctaac atctcaaaat ttggcccttg taaagtacca tcctttgaaa
     8401 tatccgtaca tatcaatgtt ttgacaccaa tcttttccat tcttttcgca aaatccaagt
     8461 agtattcttt acttttttct agccagcctg aagtacagac ataaccatct ttacaatcta
     8521 agcccaccgc aatctttgat ccatattttt taattgcatc ttttaataag caatcatctt
     8581 gaatagcaga agttcctaaa atcactctgg atacaccatt ttcaatataa aaatcaatgg
     8641 cttccatagt acggattccc ccaccgactt caacaggcaa ttggcaagtc tttacaactt
     8701 cacaaattaa atccgcattt acacgacttc cttcttttgc gccatttaaa tcaaccacat
     8761 gtaaataatt agccttttcc ttttcaaatt tttttgcgca agccaataca tcttcagcca
     8821 cacattttga ctgaccataa tcaccttgat ataaacgaac tggctttcca tctaatatat
     8881 ctattgcggg taatacaatc atttagaact cctttccaaa atattccaat atttttaatc
     8941 catcttttcc acttttttca gggtgaaact gacatcccat tatatgtccg tgcataacta
     9001 agccaggtat tttcatattt ccatacatgg aatatccaat tagatcttga tcttccatat
     9061 ctttggcata ataagaatgc acataataca catacgtgtt ttctttaaag atgttttgtg
     9121 cctgactact ttctaaaaca ttccatccaa tctgaggaat tttaacactt gtatcttcca
     9181 tatgacaaac atggcctttt ataaagccaa agcctaggca atatccattt tcttcactcg
     9241 attcaaacaa ggcttgcatt cctaaacaaa ttcctaataa aggaacatct tttttcactt
     9301 gttgaacaac aaaatcgtat aatcccatgg aatgtaaggt gttcatgcaa tcttgaaaag
     9361 ctccaacacc tggtaagatc aatttatcac atttagataa aacagaaaca tcttttgaaa
     9421 caacactatc taaatttaaa taatcacaag cattttttac actgtgtaaa ttggacatac
     9481 cataatctat aattccaatc attctaacac tccttttgaa ctaggaatct tatctgattc
     9541 tatttttaca gcttgtttta aagcacgacc aaacgcttta aatatagctt ctactttatg
     9601 atgatcattc tttccataga acacattgat atgtagtgta acacctgcag caacactgaa
     9661 ggctctaaag aattcttcaa ccatttcaca agacatcgtc ccaatatttt cacgattgaa
     9721 atcacaattg aagactaaat atggacgtcc actaatatca agtgtgacat tacataatac
     9781 ttcatccata ggtaaattca tacttccata acgggcaatc ccttttttat cgcccaatgc
     9841 ttctaagaac aatttcccta atagtatacc gcaatcctca atggtatgat gatcacatac
     9901 ttctaagtcc ccatctgcct taacaactag atcaaaatta gagtggaacg cgaacaatgt
     9961 caacatatga tcaaaaaagc caattcctgt atcaatttca ctatttccat ttccatctaa
    10021 atttatcttt tcatagattt ttgtttcctt tgtatctcta cttttttcag ctgttcgcat
    10081 actcaaatct ccttagaaca tctaatacca tttcattttc ttcttttgta ccaatcgtta
    10141 tacgaaaact tgcatcttca taatttcgaa tcgtgatatt cttttcttta aacatcgcaa
    10201 gcaacaaatc ctttttttca cttcttccaa ataagaaatt cgcattggaa ggatacattg
    10261 tcagttgctt aaattccttt acttcttgat acattctttc tctttctgaa ataattgtcg
    10321 caatagtatc cttatactca tctgcatgtc ttaagacaac acttgcgata gtttgtgtca
    10381 cactacttaa ggcataagga ataaagagtg cacttaactt ttcaacttgt gaagaaatca
    10441 taaagccaca acgaatccct gcaagaccct atgctttgga taatgttcga catacaaata
    10501 catttggata ctctttcaat aaacaaactg cactttcttt accaaactcc atataagcct
    10561 catcaaagat aacaggaatg gttggcagac gaatggcgga tggcagcatt cagatattgg
    10621 cttgcgccaa ggaaaactcc ggcgattgca ttcgcagagg aaccgttcat aatgtagaca
    10681 aaacaagtga gtgccttaag aacataaagc agcgtcttca ggatgtggta aagaaaaaca
    10741 tttctaaagt ttatgtttgc ataggcgggc agagtctgca ttctgtatta aacaccgttt
    10801 caagagattt cgagaccgaa acgaagataa ctcaagagat agttgatgct tt
//
LOCUS       DBMDWL010000063        10756 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2122_length_10756_cov_2.234256, whole genome shotgun sequence.
ACCESSION   DBMDWL010000063 DBMDWL010000000
VERSION     DBMDWL010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2122_length_10756_cov_2.234256"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(93..>274)
                     /locus_tag="ACZPDN_05705"
     CDS             complement(93..>274)
                     /locus_tag="ACZPDN_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298150.1"
                     /translation="THKENPWLIARNGYGDDIPSNELITKDSIKNYFIKICNEYDISK
                     EEDIHKYILKLSDIV"
     assembly_gap    275..284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<285..1055)
                     /locus_tag="ACZPDN_05710"
     CDS             complement(<285..1055)
                     /locus_tag="ACZPDN_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin SocA
                     domain-containing protein"
                     /protein_id="HIR1298151.1"
                     /translation="MQRETDFCVECREMSSYSLQKRCVTKNIRGENLTFEITCAVCDT
                     CGGFMSVPGIIDLNVHEIDEQYRKLKGLVSIEDIHTLMELYNIGKAPLSIVLGFGEIT
                     ITRYLLGQVPSKEYSNIIRNALSSPVYMEQKLLENKDRVALAAFKKSMNRVSELKNMF
                     IISNKMIGVISYIFEKLDEVTPLMLQKLLYYIQGLSFVLNGREMFEENCEAWVHGPVY
                     KDVYNIFKKFGFNVIDDPKFIMFEGYKKYLDDEDKYIID"
     gene            complement(1076..1405)
                     /locus_tag="ACZPDN_05715"
     CDS             complement(1076..1405)
                     /locus_tag="ACZPDN_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298152.1"
                     /translation="MDGKFRIALNSNRLTNLSIFDEYLINEESVKSILLNLTVYDFCE
                     KVQNKHANFNHEWLYVFGKEINLIKRFEEKSEMVPLYIKFNKIEDKFLIVVSFHKQKY
                     PLVYYFQ"
     gene            complement(1572..2273)
                     /locus_tag="ACZPDN_05720"
     CDS             complement(1572..2273)
                     /locus_tag="ACZPDN_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HIR1298153.1"
                     /translation="MKKLVKFIFKLLLILIIGFVGVFFGKGYWNYLQLTETKSVDEVV
                     NEVRASPYYVDYDDISPNLIRATIAIEDRRFYDHGGFDYIGLVRAAVSQFDEDLLTSG
                     GSTITQQLAKNLYGMFESSWDRKSTEFFVARYLEKHYSKNDIVTLYVNVINYGNNYTG
                     IYEASMGYLDTLPEDLTVAQASLLAGIPQSPSHYELVNHFTQAKQKQYAVLKAMAECG
                     YILESDIATYYNASV"
     gene            complement(2326..3000)
                     /locus_tag="ACZPDN_05725"
     CDS             complement(2326..3000)
                     /locus_tag="ACZPDN_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR1298154.1"
                     /translation="MYKISELYDLDHTLAKDYLAQFEYPWQALAGIKELILELGKNLG
                     EDYVEVKEHVWVHKTAHVFESAYLGAPCIIGPKTEVRHCAFVRGSALVGANCVVGNSV
                     ELKNCILFDNVETPHYNYVGDSILGYHSHLGAGGITSNLKSARDNIILRRKDENYNVV
                     EEFETGLRKIGAFMGDYVEVGCNSVLCPGTIIGPHTNVYPLSRVRGQIAANSIFKDAT
                     HVTLKD"
     gene            complement(3016..3483)
                     /locus_tag="ACZPDN_05730"
     CDS             complement(3016..3483)
                     /locus_tag="ACZPDN_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1298155.1"
                     /translation="MKIVVVSDSHGRNDILNAIREKHPDAGLFIHCGDLEDDPMFYPG
                     YIVVRGNNDYYGRFEDERIIPIGKHRIYVTHSHRFSYFSRSEQLSNRAKALDCDIVCF
                     GHTHVAYLDQVNGITLLNPGSLSHARDGRPCSYAILDIDENEIRVEFKFEPEW"
     gene            complement(3493..4533)
                     /locus_tag="ACZPDN_05735"
     CDS             complement(3493..4533)
                     /locus_tag="ACZPDN_05735"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1298156.1"
                     /translation="MISRKEMRARVLLIVICFAVGCFCLYHMDKSYDPLARYPYTTDA
                     NRDIILEYLDSNDIDYLINQHITPDKFMDFIKLDGFSLKNTLYYREAKDTQDADNEYI
                     VNFVNRFRKNFSYDSLKELLSHYSYIDLTTYYENEAVLYSDLRLVADPTNPYVVLNQE
                     NTVYKYAPENLVDFNGIYVQNAMVDNLSSMLDAYASVMDGQDHLSVSSGYLSYEQVLD
                     QYVNASKEFAGVDHYMYGAGKNEQQLGYTIVLDGQNEWIELCKQHLNEDMDYTQVEEE
                     LSKENKNRVEWILENAYRYGFVVRYGKGHEKKTGHWYLPFVLRYVGIENAKILHDGDK
                     SMEQMSFQDELN"
     gene            complement(4569..6326)
                     /gene="uvrC"
                     /locus_tag="ACZPDN_05740"
     CDS             complement(4569..6326)
                     /gene="uvrC"
                     /locus_tag="ACZPDN_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR1298157.1"
                     /translation="MSISKHLQDKLALLPDAPGCYIMKDENQNILYVGKAKVLKNRVR
                     SYFHGTHNNKTTRLVSHIRDFEFIVTGSEKEALLLEINLIKKHTPPYNIMFMDDKTYP
                     YIEITKENNFVVRTTRNIKSKKSEYFGPYPSSQSAYEIVRLINQIFSVRKCRHIPDSP
                     CLYYHMHQCLGPCIHEIDPKENEKTRKKIKKFLSGDTKEVMDSLQAKMLDASENLQFE
                     KAQEIYNTIQALEHVIEKQTIDFKDLANRDVFGYYVDSGYISFQGFFIRQGKLLERNM
                     SITPLYEDEMDAFTSFIMQYYEKNVVPKEILVPQGTPVDVLKEALNTNVHIPIRGEKK
                     KLIEMVYKNAKEAHEQKFQLVFRKDNELKKANAMLSKIFDANIHTVEMFDNSHIQGTF
                     NVSGLVVFKDGIPDKNSYRHYKLDGYRSDVDSMKEVIYRRYFRLLKEHLPMPDLLLVD
                     GGAAQISAAKEIIDMLQLDLRVAGLVKDDKHATRALMDVNLNEIPIDSKSSLFFLLTR
                     MQDEVHRFAITYHRKLRDKSMTKSVLDTVSGIGPKRKKELLKHFKSMKQMRQASVEQL
                     SEVIPEDVAKILYARLHEK"
     gene            complement(6335..8650)
                     /locus_tag="ACZPDN_05745"
     CDS             complement(6335..8650)
                     /locus_tag="ACZPDN_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR1298158.1"
                     /translation="MKRNFRDELNQAIDFSSVLTQICAFSSFSCSKEKILNALPQFNK
                     LEIQEQLNYAKEAIQFEQKGGLLNLSGANDISLPVSKAEKQMTLTSKELISIYHFLTA
                     VKQAKQSLNSSEFIELTNLAQSMDGCTRLMDSIISKIDLTGSVKEDATPGLKSMHKAL
                     VDTRLALQSKSRQFLKKNSSKLMENMTTTVSGRVVVLVRAQDKNAFGGMIHGQSSSGL
                     AYYVEPSSFVADNNEISSLVIRIEEEKKRICKELSMQVKKHALALTSALETLTIIDVA
                     FTKAKWAIRYDGCIPSLQSRDHSLYLEHAKHPLIDEKKVVCNTYELNDQQACLMISGP
                     NMGGKTVTLKTIGLFVALAHAAFPVLCHKAILPFYQSMYFDIGDHQSIENNLSTFSSH
                     ISRLSHICQKSDENSFILLDEIGNGTDPLEGASLAVAILEYLISKKSTIITSTHYSQV
                     KTYGKANEHVLVSSVEFDPETLKPTYKYIPGVSGASYAFHIAREYHLEESILKRADFL
                     KNENEKQTEKELEKLEKLQNDVLKQKERFNALIEDAHRVQKEAYEKEKEIEKRKAELD
                     ASYQEQLNEMLEKKKAQAKEILTVLRKQKTGKQHEQIEKMHELDLLNEHVVEDVEEEK
                     KEFKVGDYVKITGLNSHGEIVDIRRNEATVLTNGMKMKVKLSKLEPMHKPQIKKTTYK
                     AHVESVSSRFPLELNLIGMRVEEGIAALDKYLDQAVVKHIKQVRIIHGMGTGALRTAV
                     WKDLKKQPNVSKFTSAGPSEGGLGATIVILK"
     assembly_gap    8876..8885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8886..9657
                     /gene="rnhC"
                     /locus_tag="ACZPDN_05750"
     CDS             <8886..9657
                     /gene="rnhC"
                     /locus_tag="ACZPDN_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HIR1298159.1"
                     /translation="ENTPPYTYYQIKTKECTITAYTSGKVVFQGADLSWLEPATPQKE
                     AQDQAGSDEVGTGDYFGPVVVASCIVTKEAREKLSHLGIQDSKQVDDTKIRKLAPIIK
                     EVCPHSILIVPNSKYNDMHDSCNMVDMKCRLHNQAYVNLIHKGYTLPKQIVIDQFVQE
                     KSYYRYLQGFPEVIRGITFETKAENKYLAVACASILARNAFLEAWDLMEEKYDFKFEK
                     GAGSKVDACGKEFVKKFGKEKLYQVAKLHFKNTQKIGI"
     gene            complement(9654..>10756)
                     /locus_tag="ACZPDN_05755"
     CDS             complement(9654..>10756)
                     /locus_tag="ACZPDN_05755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1298160.1"
                     /translation="SALILKKNYMLMVICEVVILLLYCGFYIAKVRRISNKSNEFEKD
                     LNVSHTLIQCVRKLSNSTNQKGAIDDILKTICEFYQAERCYVLDLDFENKQTNTIYEY
                     GNRNDDIHIDNMVRLCLENMDLVQRFFENQKSYYIENVTSEISKTSPIYSSFISQKIH
                     SIILVPFVDNHQIKGVFAVDNPKQNYYQKDFLESLCFFIETAMTREKEKKHLQNLSYV
                     DSLTYAQNRNHFNEYLKKNRCKELHFVGVIYLDLNGLKEINDKMGHLAGDTLIISASY
                     VLQEIFADNSYRIGGDEFVVIEQDVLESEFYSKYEMLLKRMKELEISVATGYIWKERC
                     LNLSEILQAADQKMYEDKKRYYSIAQKDQRRR"
BASE COUNT         3547 a         2283 c         1355 g         3551 t
ORIGIN      
        1 tagaacacca gattacctac atactcttat acgtaatttt ttcaacaaat tctaggaaag
       61 atgaaaaaaa tgcatccatt taaacagatg cattaaacaa tatcactcaa ttttaaaata
      121 tacttatgaa tatcttcttc ctttgaaata tcatactcat tacatatttt aataaagtaa
      181 tttttaatag aatcctttgt gatcaattca tttgaaggta tatcatcacc ataaccatta
      241 cgagcaatta accatggatt ttctttatgt gttgnnnnnn nnnnatctat aatatattta
      301 tcttcatcat ctaaatattt tttatatcct tcaaacataa taaatttcgg atcatcaatc
      361 acattaaaac caaacttctt aaatatatta tacacatctt tataaaccgg tccgtgtacc
      421 caagcctcgc aattttcttc aaacatttct ctaccattta acacaaacga caatccctgt
      481 atataatata ataatttctg aagcattaat ggagttacct catctaattt ttcaaatata
      541 tacgaaatga caccaatcat cttattcgaa ataatgaaca tattttttaa ctcacttact
      601 ctattcatcg attttttgaa tgctgccaaa gcaactctat ctttattttc caatagtttt
      661 tgttccatat ataccggaga cgataacgca ttgcgtatga tatttgaata ctcttttgaa
      721 ggcacttgcc ccaacaaata tcgcgtaatc gttatttcac caaagcctaa cacaatggat
      781 aatggagctt ttccaatatt atacaattcc atcaaagtat gaatatcttc tattgaaact
      841 aaacctttta actttcggta ctgctcatct atttcgtgga catttaaatc gataatacca
      901 ggaacactca tgaatccacc acacgtatca caaacagcac aagtaatctc aaaagttaaa
      961 ttttctccac gaatattttt tgtgacacat cttttttgta atgaataaga cgacatctct
     1021 ctacactcaa cacaaaaatc agtttctctt tgcatactac aattacctcc ttcttttatt
     1081 gaaaataata caccagtgga tatttttgtt tatgaaaaga taccacaatc aaaaacttat
     1141 cttctatttt attaaactta atatacaatg gaaccatttc acttttttct tcgaatcttt
     1201 taatcaaatt aatttccttt ccaaaaacat atagccattc atgattaaaa ttcgcatgtt
     1261 tattttgaac cttctcacaa aagtcatata ccgttaaatt caacaatatt gatttaacac
     1321 tttcctcatt tatcaaatat tcatcaaata ttgacaaatt tgttaatcga ttcgaattca
     1381 aagcaattct aaacttacca tccaaaatca gttttttgct tcgcctaaat aatcattaat
     1441 ttcagattca tcaatcaaaa gattcacctc cactttatga tggcattttc ttctttctct
     1501 atcacaatta tatcatatga tatatttttg taaatatcct atcataaaga atataaataa
     1561 aaatgcatcc attaaacaga tgcattataa tatgtcgcaa tatcactttc caaaatatac
     1621 ccacactctg ccattgcctt taataccgca tattgtttct gtttggcctg cgtaaaatga
     1681 ttgaccaatt cataatgact tggagattgc ggaatacccg ccaataaaga agcttgcgca
     1741 accgttaaat cttcaggaag cgtatccaaa taacccatac tcgcctcata tatgcctgta
     1801 taattatttc cataattgat cacattcaca tataaagtaa caatatcgtt ttttgaataa
     1861 tgcttctcta aataacgcgc cacaaagaac tctgtactct tacgatccca tgaactttca
     1921 aacatcccat acaagttttt cgcaagttgc tgcgtaatcg tagaaccacc actcgttaat
     1981 agatcttcat caaattgaga aacggccgca cgaaccaagc caatataatc aaaaccacca
     2041 tgatcataaa aacgacgatc ctctatcgca atggtagccc gaatcaaatt tggtgatata
     2101 tcatcataat ccacataata aggacttgcc cttacttcat tgactacctc atcaacggat
     2161 tttgtttctg taagctgtaa ataattccaa taccccttgc caaaaaagac gccgacaaaa
     2221 ccaataatta gaatcaacaa caatttaaat ataaacttaa ctaacttttt catacctaaa
     2281 ccttaacata agaaaaagaa cttgtaaaat tacaagtccg taagattaat cttttaaagt
     2341 tacatgtgtt gcatctttga aaatagaatt tgccgcaatc tgaccacgca cacgagataa
     2401 tggatataca tttgtatgtg gaccaataat cgttcctgga caaagaacag aattacatcc
     2461 aacctctaca tagtcaccca tgaaagcacc gatttttctt aatcccgttt caaattcttc
     2521 aaccacatta taattctcat cttttcttct caagatgata ttatcacgag cactcttcaa
     2581 attacttgtg ataccaccag cacctaagtg agaatgatat cctaaaatac tatccccaac
     2641 atagttatag tgaggggttt ctacattgtc aaataagatg cagtttttta attccacact
     2701 atttccaaca acacaatttg ctccaaccaa agcactacca cgaacaaagg cacaatgacg
     2761 cacttccgtt ttaggaccaa taatacatgg agcacctaaa taagcacttt caaatacatg
     2821 agcagtttta tgaacccaaa catgctcttt gacttccaca taatcttccc ctaagttttt
     2881 tcctaattcc aaaatcaatt ctttgattcc agctaaggct tgccaaggat attcaaactg
     2941 tgccaagtaa tccttagcca aagtatgatc taaatcataa agctcactaa ttttgtacat
     3001 aaatttcctt ctttcttacc attcaggctc aaacttaaat tcaacccgaa tttcattttc
     3061 atctatatct aaaatcgcat aactacatgg tcttccatcc cttgcatgcg ataaagatcc
     3121 tggattcaat aatgttattc cattcacttg atccaaatag gcaacgtgcg tatgtccaaa
     3181 acatactata tcacaatcca atgcttttgc cctatttgaa agctgttcgc ttcttgaaaa
     3241 atacgagaaa cgatgcgaat gcgtaacata aatacgatgc ttaccaattg gaataattct
     3301 ttcatcttca aaacgaccat aataatcatt gtttccacga acaacgatat agcctggata
     3361 aaacattgga tcatcctcta aatctccaca atgaataaat aatcctgcat ctggatgttt
     3421 ttcacgaatc gcatttaata tatcatttct tccatgagaa tcactcacaa ccacaatttt
     3481 cataaattac ccctaattca gttcatcttg aaaagacatt tgttccatac tcttatcacc
     3541 atcatgcaag atcttagcat tctctatacc cacatatcga agtacaaatg gcaaatacca
     3601 atgtcccgtc ttcttttcat gaccttttcc ataacgcacc acaaaaccat agcgatacgc
     3661 attttccaat atccattcta cacgattttt attttcctta gacaattctt cttcgacttg
     3721 cgtatagtcc atatcttcat ttagatgctg cttacaaagc tctatccact cattttgacc
     3781 atctaaaaca atcgtatacc ctaattgctg ctcattcttt ccagccccat acatataatg
     3841 atcaacaccc gcaaattcct ttgacgcatt gacatactga tctaagactt gttcataact
     3901 caaataacca cttgaaacac tcaaatgatc ttggccatcc ataacggaag catacgcatc
     3961 gagcatactt gacagattat caaccatagc attttgaaca tatataccat taaaatccac
     4021 taaattttct ggtgcatatt tatatactgt attttcctga tttaagacaa catatggatt
     4081 tgtaggatct gccaccaacc ttaaatctga atataggaca gcctcatttt cataatatgt
     4141 tgttaaatca atataggaat agtgcgacaa caattccttt aaagaatcat acgaaaaatt
     4201 cttacggaaa cgattcacaa aattgacaat atattcatta tccgcatcct gcgtatcttt
     4261 tgcctctcta taatacaacg tgttttttaa actaaatcca tctagcttaa taaaatccat
     4321 aaacttatct ggcgtaatat gctgattgat caaataatca atatcattac tatctagata
     4381 ttctagaatg atatcacgat ttgcatccgt cgtataagga tacctggcta acggatcata
     4441 ggacttgtcc atatggtaaa gacaaaaaca tcccaccgca aaacaaatca caatcaataa
     4501 aacacgtgct cgcatttctt ttctactaat caaaataagt acctcacgca aaacattata
     4561 ctaaactatc atttttcatg caaacgagca taaagaatct tagccacatc ctcaggaatc
     4621 acttcgctaa gctgttctac actagcttgt cgcatctgct tcatactctt aaaatgcttc
     4681 aacaattctt ttttacgctt aggaccaata ccacttaccg tatctaacac tgattttgtc
     4741 atagacttgt ctcttaactt tctatgatac gtaatcgcaa aacgatgtac ctcatcctgc
     4801 atacgcgtca acaaaaagaa taaactcgat ttggaatcaa taggaatttc atttaaattt
     4861 acatccatca aagcacgcgt cgcatgctta tcatccttaa ctaaacctgc cactcttaaa
     4921 tcaagctgca acatatctat aatttctttt gcggctgaaa tttgagcggc acctccatcc
     4981 accaacaata aatctggcat cggcaaatgc tctttcaaca acctgaaata tctacgataa
     5041 ataacttcct tcatactatc gacatcactt ctgtagccat ccaatttata atgacgatac
     5101 gaattcttat caggaattcc atccttaaaa acaaccaacc cactcacatt aaaagtccct
     5161 tgaatatgcg agttatcaaa catttctacc gtatgaatat tggcatcaaa tatttttgac
     5221 aacatagcat tggccttttt aagctcatta tccttacgaa ataccaactg aaatttttgt
     5281 tcatgcgcct ctttcgcatt cttatacacc atttcaatca attttttctt ttccccacga
     5341 attggaatat gcacattcgt atttaaagcc tcttttaaaa catcgaccgg cgtaccttgt
     5401 ggcaccaaaa tttcttttgg cactacattc ttctcataat actgcatgat aaaacttgta
     5461 aaagcatcca tctcatcttc atataaaggc gtaatggaca tatttctttc caataatttc
     5521 ccttgtcgaa taaagaaacc ctgaaatgaa atatagccgg aatccacata atatccaaac
     5581 acatcgcgat tggccaggtc tttaaaatca atcgtctgct tttcaatcac atgctccaat
     5641 gcctgaatcg tattataaat ttcctgcgcc ttttcaaact gcaaattttc actggcatcc
     5701 aacatttttg cctgcaaaga atccatcacc tctttggtgt ctccactcaa aaatttctta
     5761 atttttttac gcgtcttctc attttcctta ggatcaatct catggataca aggccctaaa
     5821 cactgatgca tatgatagta caaacaagga ctatccggaa tatgacgaca ctttcttaca
     5881 gaaaagattt gattgatcaa cctcacaatt tcataagccg actgactgct tggatacggc
     5941 ccaaaatatt cactcttttt acttttaata ttacgcgttg tacgaaccac gaaattattt
     6001 tcctttgtga tctcaatata cggatacgtc ttatcatcca taaacataat attatatgga
     6061 ggtgtatgct tcttaattaa attgatttcc aataataaag cctctttttc actgcccgtc
     6121 acaataaatt caaaatcacg aatatgcgaa actaaacgcg ttgtcttatt attatgtgta
     6181 ccatgaaaat aagaacgaac acgattcttt aatactttgg cttttcctac atacaaaata
     6241 ttctgattct catccttcat gatgtaacag ccaggtgcat ccggaagtaa agccaattta
     6301 tcttgtaaat gcttagaaat actcatttta tcccctactt taatatgact atcgttgctc
     6361 caagtccacc ttcactagga cctgcacttg taaatttaga aacatttggt tgtttcttta
     6421 aatccttcca aacggctgtt cttaaagccc ccgtacccat accatgtatg atacgaactt
     6481 gttttatgtg ttttacaaca gcctgatcca aatatttatc taatgccgca attccttctt
     6541 caacacgcat accaattaga ttcaactcta aaggaaagcg actcgataca ctttctacat
     6601 gcgctttata cgttgtcttt ttgatttgtg gtttatgcat aggctccaac ttagaaagct
     6661 tgaccttcat cttcattcca tttgttaaaa cagtcgcctc atttcttcga atatctacaa
     6721 tttcaccatg cgaattcaaa cccgtgatct ttacataatc ccccactttg aattctttct
     6781 tttcttcttc aacatcttca accacatgct catttaaaag atctaactca tgcatctttt
     6841 caatctgttc atgctgctta cccgtcttct gtttacgaag cacagttaaa atttctttag
     6901 cctgcgcctt tttcttttca agcatctcat ttaactgttc ttgataggaa gcatccaatt
     6961 ctgccttacg cttttcaatt tctttttctt tttcataagc ctctttttga acacgatgcg
     7021 catcctcaat caatgcatta aaacgctctt tttgtttcaa tacatcattt tgaagcttct
     7081 ctaatttctc taattctttt tccgtctgct tttcattttc attctttaaa aaatccgctc
     7141 tttttaaaat agattcttcc aaatgatatt ctctcgcaat atgaaacgca taactagccc
     7201 cagatacacc cggaatatat ttataggttg gcttaagcgt ttctggatca aattcaacac
     7261 tcgataccaa gacatgttca ttggcctttc catacgtttt tacttgcgaa tagtgcgttg
     7321 aagtaatgat cgtactcttt tttgaaatca aatactccaa tatcgcgacc gcaagactgg
     7381 ctccttctaa aggatctgta ccatttccaa tttcatccaa taagataaaa ctattctcat
     7441 cactcttttg acaaatatga gaaagcctag aaatgtgact tgagaaagtc gataaattat
     7501 tttcaatgga ttgatgatct ccaatatcaa aatacatcga ttgataaaat ggcaagatgg
     7561 ccttatgaca taatacagga aatgccgcat gagctaatgc cacaaataga ccaatcgtct
     7621 ttaaagttac tgttttacca cccatattcg gtcctgaaat cataagacaa gcttgttgat
     7681 cgtttaattc atatgtgtta caaaccactt tcttttcatc aatcaacgga tgcttggcat
     7741 gctctaaata caacgaatga tctctagatt gtaatgaagg aatacatcca tcatagcgaa
     7801 tggcccattt tgcctttgtg aaagccacat caatgattgt taaagtttct aatgccgaag
     7861 ttaatgcgag tgcatgtttc tttacctgca tagacaattc tttacagatt cttttcttct
     7921 cttcttcaat acgaatcact aaggatgaaa tctcattatt atccgctaca aaactcgatg
     7981 gctctacata ataggcaaga cccgaactgg attgtccatg aatcatacct ccaaaagcat
     8041 ttttatcttg ggcacgaact aatacgacaa cacgtcccga aaccgttgtc gtcatatttt
     8101 ccattaattt tgaactattc ttctttaaaa actgtctcga tttggactgc aaagctaaac
     8161 gcgtatctac caaagcctta tgcatactct ttaatccagg tgtcgcatct tctttaacac
     8221 tccccgttaa atcgattttg gaaatgatgg aatccatcaa acgtgtacat ccatccatcg
     8281 attgagctag attggttaat tctataaatt cagaagagtt taaactttgt ttcgcttgct
     8341 taacagccgt taaaaaatga tagatggaaa ttaattcttt acttgttagt gtcatctgtt
     8401 tttcagcttt tgaaacaggt aaagaaatgt cattggcacc tgataaattc aataaaccac
     8461 ctttttgttc aaactggatg gcttctttag cataattcaa ttgttcttgt atttctaatt
     8521 tattaaattg aggcaacgca ttcaaaatct tttctttgga acaagaaaaa cttgaaaatg
     8581 cgcaaatctg agttaataca ctcgagaaat caatagcctg atttagttca tctctaaaat
     8641 ttcgtttcat aaaatactcc attcctacct attttaacac aaattttatg gtaaactaaa
     8701 taaggtgatt aaaatggcaa cattgacaaa gaatatgaca aaagaagaaa tctttcaatt
     8761 aaaaaaccga ttgtcttctt ttattttaaa agaaaattcc gcgtaaaaat cccccgaaag
     8821 attccttccg ggggtaatga gcacgtatga attaatcgtg ttcttctctc atccannnnn
     8881 nnnnnagaaa atacacctcc atatacgtat tatcaaatca agacaaaaga gtgtacaatt
     8941 acagcttaca caagtggtaa agttgtattt cagggagccg atctttcttg gttagaacct
     9001 gcaacgcctc aaaaagaagc acaagaccag gctggaagtg atgaagttgg tacaggcgat
     9061 tattttggtc ctgttgtagt ggctagttgt atcgtcacaa aagaagcaag agaaaaatta
     9121 tctcatttag gtatacaaga ttccaaacaa gtggatgata ccaaaatcag aaaactggct
     9181 ccaatcatta aagaagtctg tccacacagt attttgattg tgcccaattc aaaatataac
     9241 gatatgcacg attcttgtaa tatggtggac atgaaatgtc gtttacacaa ccaggcctat
     9301 gtaaatctaa tccataaagg atatacgctt cctaaacaga ttgtcatcga tcaatttgtc
     9361 caggaaaaaa gttattatcg ttacctacaa ggttttcctg aagtgattcg cggcattact
     9421 tttgaaacaa aggccgaaaa taagtatctt gcagtagctt gtgcaagcat tcttgctcgc
     9481 aatgcctttt tagaagcttg ggatcttatg gaagaaaaat atgattttaa attcgaaaag
     9541 ggggctggta gtaaagtgga tgcttgtggc aaagaatttg tgaaaaaatt tggaaaagaa
     9601 aagctttacc aagtggcaaa gcttcatttt aaaaatactc aaaaaatcgg tatttaacgc
     9661 cttctttgat ccttttgcgc tatggaataa tagcgctttt tatcttcata catcttttga
     9721 tcggctgctt gtaaaatttc actcaaattt aaacaacgct ctttccatat ataccctgtc
     9781 gcaacactaa tttctagttc tttcatacgc ttcaacaaca tttcatattt agaatagaat
     9841 tccgattcca aaacatcttg ttcgataacc acaaattcat ctccaccaat acgatatgag
     9901 ttatccgcaa aaatttcttg tagtacataa gaagcactaa taatcaaagt atctcctgca
     9961 agatgtccca ttttatcatt gatttctttt aatccattta aatccaaata aatcacgcca
    10021 acaaaatgaa gttctttaca cctatttttt ttaagatatt cattaaagtg attgcgattt
    10081 tgcgcataag tcaaagaatc gacatagctt aaattctgta aatgtttctt ttctttttca
    10141 cgcgtcatgg ccgtttcaat aaagaaacat aaagattcta aaaagtcttt ttggtagtag
    10201 ttttgtttag ggttgtctac cgcaaaaaca cctttaattt gatgattatc cacaaatggt
    10261 acaaggatta tactatgtat tttttgtgaa ataaaacttg aataaatggg actcgtcttt
    10321 gagatttcag aagttacatt ttcaatataa tatgactttt gattttcaaa gaaacgctgc
    10381 actaaatcca tattttctaa acatagacgg accatattat caatatgaat atcatcattt
    10441 cgatttccat attcataaat ggtgttcgtt tgtttatttt caaaatctaa atccaataca
    10501 taacatcttt cagcttgata aaattcacaa atagttttca agatatcatc aatggctccc
    10561 ttttgatttg tactattcga tagtttgcga acgcattgaa tcaaagtatg cgaaacgttt
    10621 aaatcctttt caaattcatt tgatttgttt gaaattcttc gaacttttgc gatatagaat
    10681 ccacaataca ataaaagaat cacaacttca caaataacca tgagcatata attctttttc
    10741 aatatcaagg cactcg
//
LOCUS       DBMDWL010000064        10752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2125_length_10752_cov_2.613933, whole genome shotgun sequence.
ACCESSION   DBMDWL010000064 DBMDWL010000000
VERSION     DBMDWL010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2125_length_10752_cov_2.613933"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="ACZPDN_05760"
     CDS             <1..749
                     /locus_tag="ACZPDN_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015154.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR1298161.1"
                     /translation="LFLEHYDIYDVSYNWGWKYKSSTKIFKSYIDYWNELKVKATKEG
                     NKPLRTIAKLMLNSLYGKFAASPEGRSKIPYLDNNIVKYKLSELEERTAYYLPISIFI
                     TSWARDKTIRSAQAVYHRFIYADTDSLHLEGTDIPENLLISDTELGKWKIESTFKRGK
                     YLRQKCYIEDAVTPVDEIEKFKKENPECLHLVSKDSIINIVCAGMPKGCYKNVTWENF
                     DYGSVFDGKLGVKHTDGGIVLVDTTFTIKG"
     gene            807..1904
                     /locus_tag="ACZPDN_05765"
     CDS             807..1904
                     /locus_tag="ACZPDN_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017687.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HIR1298162.1"
                     /translation="MCDGQRVKAYHIFRGDSWYNASYFLFMYWNINQILAYQRNFNFI
                     NGTRSIGKTYTTQKFCLKKCIENGWQAVYIVRYASEKEEGALPLAFEKVLENEFKDYL
                     WKLQNEKIQYKNENGEWVDIFICIALSESQKIKKRSFPFVKFIIFDEYMLERENNSKY
                     VKGWKEPEIFLNIYHTIDREEDRVICFLLGNTTTFFNPYHMYPSFDIPSNIKPGEIWY
                     NKFCLFQWAIPDEEMIEKKSKSKFNEMIGGSEYGRYARDGEYVEDSEEFVLSRPQSAK
                     HLMNIKSNDTIYGIWFDRINGNVFIDDKYDPSCKFNFVLSKEELAEGFILGTKDFYLI
                     KWLSQKFKNGFVKYTTLETKKKMERVIYYLS"
     gene            1918..2151
                     /locus_tag="ACZPDN_05770"
     CDS             1918..2151
                     /locus_tag="ACZPDN_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298163.1"
                     /translation="MGWAENFVTIVNGVGFPIVVCVFLVWYIYKENKRRREEKEEYEN
                     KQNDLYLKLSQSVDNNTAALNKLLMQMEDKNNG"
     gene            2144..2866
                     /locus_tag="ACZPDN_05775"
     CDS             2144..2866
                     /locus_tag="ACZPDN_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1298164.1"
                     /translation="MDNIGLIAYCRNMCYNEKTLYMWGGLMNAITEYFINYKAKQYPS
                     RYTESRKETLRKYINKAYGCDCVGLIKSYYFGGVGSPGYEASKDLNASGMFNKASEKG
                     PISTLPETVGIGLYQPGHVGVYAGNGIAYECTLGSYGDGVVKTKVAGRGWTNWFKVPF
                     IDYINEDDQRGDEDCNCDCNCPGCICKEDYYNYTVVSGDSFWGIAKKVYGDGNKYPKI
                     LEYNGMTENDAIYAGDILKIPV"
     gene            2881..3204
                     /locus_tag="ACZPDN_05780"
     CDS             2881..3204
                     /locus_tag="ACZPDN_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298165.1"
                     /translation="MLDKEEMKTILQNFSERFGDDETAMNELRRIQEDHEERINNESR
                     AYDSDGVLYSEKYDNLKRRYRERFFTSDEEIKEDQKEDIKKDSSKLTFESLFESREGD
                     YKKGM"
     gene            3209..4489
                     /locus_tag="ACZPDN_05785"
     CDS             3209..4489
                     /locus_tag="ACZPDN_05785"
                     /inference="COORDINATES: protein motif:HMM:NF052097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR1298166.1"
                     /translation="MPTKPIISSFKASGIDVLNAVRANASSTYQERIPVATQENIRQI
                     GNAMMTYEATQNEFLSALVNRIGRVIITSKSYTNPLRVFKKGILEYGETIEEIFVNIA
                     KAKQFDPSVAEEEVFKREIPDVNAVFHKMNLQNFYKVTVSNEQLRQAFLSSQGINDLI
                     GYIVDSLYTGAEFDEYITMKQLIVDAANNGEMYAVNIPAVSSDNTKTIVSTIKGVSNQ
                     LEFMSSTYNSMGVLTHTRKNRQVLIIDAALDAAIDVDVLAYAFNMDKAEFMGRRVLVD
                     NFGELTGVVAALVDEDWFMVYDNFIGFTENYNGQGLYWNYFYHVWKTFSTSPFSNAIL
                     FTTQDVAVTNVTVSPNNITHNTGSSQTVQFTANVTATGYAPKDVVWSTTSATATITEN
                     GFLTIPTTTDTTFTVRATSVYNSAKFGEATVTVK"
     gene            4505..6145
                     /locus_tag="ACZPDN_05790"
     CDS             4505..6145
                     /locus_tag="ACZPDN_05790"
                     /inference="COORDINATES: protein motif:HMM:NF044352.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298167.1"
                     /translation="MADFVPSTIVKVLKDVPLDSTYSDTIKFTSVGAQTAFFSGKAKY
                     SFTDFTYQRVNSSVSAPRGPRSIRVPRVADDLYDCNYVMFQNANYGTKWFYAFIKQVN
                     YINPNNTEIIYELDHYQTWAFDFTVLPSFVEREHPESDNLYENLIPENMNESDMYTQQ
                     VTELDLQTNFAKIVVGVSTDPGGQIVAGKLSGGVYSGVELHQFDNAEGATAFIESYGK
                     KAMADAIVCVYMSAWDVNISDLKNVTISRPTNLCGYTPRNKKLLTYPYCKITMSNRQG
                     TEYDFYYEYFSRIGNQEVETTDIIFNYLTYGGIQPKGYCVAVNYNGLQGDVNMDYNAV
                     GEVDGYVQCAWTSNAFANWLAGEGTAQAFKIGTNALATANKNTFSSGLSTMLSAFSGN
                     VGGTVSGVQSMATGTVNNLVNTVHSAADLGIDIWTRSKCPGLSKGTPHTPSLNLIYRN
                     IGFTIKQMAIRPEIAKSIDDYFDMYGYATNKLKVPNMEGRESWNYVKTKDVIISGSLP
                     VDSMDAVKKMFNSGIRFWHGDFVGHYSRSNKPMKDVKK"
     gene            6145..>6831
                     /locus_tag="ACZPDN_05795"
     CDS             6145..>6831
                     /locus_tag="ACZPDN_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017190.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298168.1"
                     /translation="MSRRKPYKLFDRWANRKNWDPYILNNETFMDYFYRLEEICINMF
                     SWENLPETIDYRFLELTLCEYGFAVYFNEEDVGNVALTCALGGPLSIYREPIYRRAYA
                     NNGFSRELDNTNSVLIYNNYLHHPSIPTIILFARRLAEIERTIDVNVKSQKTPVVITC
                     DESQLLTIKNAYKDMDENVPVIIASKNVDWKSISSLNTNAPFVSLDLNNLKRQIWNEA
                     LTFFGVSNSNT"
     assembly_gap    6832..6841
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6842..7049
                     /locus_tag="ACZPDN_05800"
     CDS             <6842..7049
                     /locus_tag="ACZPDN_05800"
                     /inference="COORDINATES: protein motif:HMM:NF017190.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298169.1"
                     /translation="ERQIGLEVSSNLGGVMAQRYIMLNSRRQAAEKINKMFGTNIKVN
                     FRQEFETFNEDMDTTTIDLNERDE"
     gene            7053..8114
                     /locus_tag="ACZPDN_05805"
     CDS             7053..8114
                     /locus_tag="ACZPDN_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298170.1"
                     /translation="MAKYTIELNELISLGFHLNLDDYPIFNEDYRAHLNKEIIEHFYF
                     REIGQETPERFNFFLKRKMAEIMPYYNQLYQSELIKFDPLSTVYMETSGDTKRKKYNE
                     NTNDYKQNNTLNASETYASNIDRSNTFKLEDTQDTTNNSNYQKSGDRTQDIVTNELTT
                     NDLKTETNTVSDSDGTTDTHGVKNTVFSDIPQAGITTTTTTAPDGTITTETTGYATTT
                     TNESTNEHSTTTAHQTSDSVATNTGTVNVDGKSNLTENWKENGSAVDVGNSKQNQNSN
                     EVTNTKEDYQKANILNEKTDRINREHGKEKENIENTEYRKGRLGVSPSDLLIKYRQTF
                     LNIDMLIINDLETLFMGVF"
     gene            8370..>10752
                     /locus_tag="ACZPDN_05810"
     CDS             8370..>10752
                     /locus_tag="ACZPDN_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021507.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR1298171.1"
                     /translation="MEKAAEANGSYYGPCEVWTEEGYSAEQGSTYTLIHKACVDRRGE
                     PIRMQLHLAYGNTTNSQIEQSIFSASKITLADKMVVAQPKGANGWYGHAIWHGAPIAA
                     GSEPTLYTVGFTRRGTMRVYSNAVSQDQLLNDTIENAMGCSGVLIQNGQITDESYQAN
                     IPSATTQTARVVMGQNMDTQEVIILCCGSYDNVQHKGMTSKACAEILLNYGCDIAVEL
                     SEGISAGAVDKGSMVFPPMNNSVPTAYCYWYISRACLYKNDYQKELAILMQNYGETLW
                     GIYESTQKINDLSNRLDKEIQDRIDGDNALNEALQAEIERAQAEEARLQGLIEQINTQ
                     ITQINQTISEIQSDISDINKEITDIKGDITTINEQILQLSNRLTALTASVTALQQTVQ
                     SIQEDITNLELAVNTLSATVTNIINGTEVLPYLSINGGTMNGDIDMANNTITNVPTPT
                     ANADAVNKKYVDDAISGSITPPTGDYLPLTGGEMHGAIAMGDFRITNLGNATSNGDAI
                     NKGQADTLYIRKAGDTTPGAQTFQNTITIGSSTDGMVMDPESGSPNLSIAAGNEAIHL
                     GVTSEKAFINSESNEIDFTAGSAGSPEYTSLRGIAEPTEDYDAANKKYVDQAVSGAGE
                     GSFLKKGGDTTTGKYSFTSSNSTLTVGPEAIQNTSSVAPGKIVLNGSSSKSIELNSNA
                     LSPNITLTNAGHSAKIIAGADSTNFIDSSNKFSFASGSSTAILDGIANPTADSQAVPR
                     GYLNSQMSDKLSIPTNGWGFTAACRYNDKIIYATGDKAYGVASGNDLAMVTDIS"
BASE COUNT         3804 a         1641 c         2116 g         3181 t
ORIGIN      
        1 agttattttt ggaacattat gatatatatg atgtttcgta taattggggt tggaaataca
       61 aatcatctac aaaaatattt aaaagttata ttgattattg gaatgaatta aaagttaaag
      121 ctacaaaaga aggtaacaag ccattaagga ctatagcgaa acttatgtta aattcgttat
      181 atggaaaatt tgcggctagt ccagaaggga gaagtaagat tccataccta gataataata
      241 tagtaaaata taaattaagt gaattggaag aaagaaccgc gtattatttg ccaattagta
      301 tttttataac aagttgggca agagacaaaa caataagaag tgcacaagca gtgtatcata
      361 gatttattta tgcagataca gatagtttgc acttagaagg tacagatatt cctgagaatt
      421 tattaatatc tgataccgaa ttaggaaagt ggaaaataga atcaacattt aaaagaggca
      481 aatatttaag acaaaaatgt tatatagaag atgcagtaac tcctgtggat gagatagaaa
      541 aatttaaaaa ggagaatcca gaatgtttac atttagtttc taaagatagt attattaaca
      601 tagtatgcgc tggaatgcca aaagggtgct ataaaaatgt gacctgggaa aattttgatt
      661 atggaagcgt atttgatggc aaattagggg ttaaacacac agatggaggt attgtattag
      721 tagatacaac ttttactata aaaggttgac aaaaaataag aaagtgatat aatggaggtg
      781 aaggaaatag ctaaggtgtt atattaatgt gtgatggtca acgtgtgaaa gcgtatcata
      841 tatttagggg tgactcctgg tataacgcca gctattttct gtttatgtat tggaatatta
      901 atcaaatttt agcatatcaa cgtaatttta attttataaa tggaacgcga tcaattggta
      961 aaacatatac aacacaaaaa ttttgtctta agaaatgtat agaaaatgga tggcaagctg
     1021 tatatattgt gagatatgca agcgaaaaag aagaaggtgc acttccttta gcatttgaaa
     1081 aagtattaga aaatgaattt aaggattatt tgtggaaatt acaaaatgaa aaaatacaat
     1141 ataaaaatga aaatggggaa tgggttgata tctttatatg tatagccctt tccgaatctc
     1201 aaaagataaa gaaaagaagt tttccttttg tgaaattcat catttttgat gaatacatgc
     1261 tagaaagaga aaataattca aaatatgtaa agggatggaa agagcctgaa atatttttaa
     1321 acatatatca tacaattgat agagaagagg atagagtaat atgtttttta ttaggaaata
     1381 caacaacttt ttttaatcct tatcatatgt atccatcttt tgacatacca tcaaatataa
     1441 agccaggcga aatttggtat aataagtttt gtctttttca atgggctata ccagatgaag
     1501 aaatgataga aaagaaatca aaaagtaaat ttaatgaaat gataggtgga agcgaatatg
     1561 gaaggtatgc aagagatggc gaatatgttg aggacagtga agagtttgta ttatccagac
     1621 ctcaatcagc taagcatctt atgaatataa aatctaatga tactatatat ggtatttggt
     1681 ttgatagaat taatggaaat gtattcatag atgataaata tgatccatca tgtaaattta
     1741 attttgtgtt atcaaaagaa gaactagcag agggatttat attaggtact aaagactttt
     1801 atttaattaa gtggctttca caaaagttta aaaatgggtt tgtcaaatac acaacgttag
     1861 aaacaaagaa gaaaatggaa agggtcatat attatttaag ttaatgaggt gatattaatg
     1921 ggctgggctg aaaattttgt tacaatagtg aatggagtag gttttccgat cgtagtatgt
     1981 gtatttttgg tgtggtatat ttacaaagag aacaaaagaa ggagggagga aaaagaagaa
     2041 tatgaaaaca aacaaaacga tctctattta aaattatcac aaagtgttga taataacaca
     2101 gcggctttaa ataaactttt aatgcaaatg gaggataaaa ataatggata atataggatt
     2161 aatcgcttac tgccgaaata tgtgttacaa tgaaaaaact ttatatatgt ggggaggttt
     2221 gatgaatgct ataacggaat attttatcaa ttacaaagca aaacaatacc cctctagata
     2281 tacagaatca cgtaaagaaa cactaagaaa gtatattaat aaagcatatg gatgcgattg
     2341 tgtaggtctc attaaatctt actattttgg tggagttggt tctccgggtt atgaagcttc
     2401 taaagatctt aatgcgtctg ggatgtttaa taaggcttct gaaaaagggc caatttcgac
     2461 tttgccagaa actgtgggga ttggacttta tcaacctggt catgttggcg tatatgctgg
     2521 caatggtatt gcatatgagt gtactttagg aagttatggt gatggagtag taaaaacaaa
     2581 agtagcaggg aggggatgga caaattggtt taaagttcct ttcattgatt atattaatga
     2641 agatgatcag agaggtgatg aagattgcaa ttgcgattgt aattgcccag ggtgtatttg
     2701 taaagaagat tactacaatt acactgtagt aagtggcgat tcattttggg gaattgccaa
     2761 aaaagtgtat ggagatggaa acaaatatcc caaaatactg gaatacaacg gaatgacaga
     2821 aaatgatgca atttatgctg gcgatatcct taaaatacct gtttaaggga gatagatttt
     2881 atgcttgata aagaagaaat gaaaacaatt cttcaaaact tttcagaacg ctttggtgat
     2941 gatgaaacag caatgaacga attaagaagg attcaagaag atcatgaaga acgcattaat
     3001 aatgaaagta gagcatatga ttctgatgga gtattatatt cagaaaaata tgacaattta
     3061 aaaagaagat atcgtgaaag attttttaca tctgatgaag aaataaaaga agatcagaaa
     3121 gaagatatca aaaaggacag ttctaaatta acatttgagt ctctctttga aagcagagag
     3181 ggagactata agaaaggaat gtaataaaat gcctactaaa cctataatta gcagttttaa
     3241 agcatctgga atcgacgtat taaatgctgt tcgtgcaaat gcttccagca catatcagga
     3301 gcggattcca gtagcaaccc aggaaaatat tcgccaaatt ggaaatgcaa tgatgactta
     3361 tgaagcaact cagaatgaat ttttaagtgc tttggtaaat cggattggca gagtaattat
     3421 tacttcaaaa tcgtatacta atcctcttag agtattcaaa aaaggaattc ttgagtatgg
     3481 ggaaactatt gaggaaattt ttgttaatat cgccaaagca aagcagtttg accctagcgt
     3541 tgctgaagaa gaagttttca agagagaaat tcccgatgta aatgcagtat tccataaaat
     3601 gaatctccaa aacttctata aagtcacggt ttccaatgaa cagctgcgtc aggctttcct
     3661 tagttctcag ggtatcaatg acctaattgg atatattgtc gattcacttt atactggcgc
     3721 tgaatttgat gaatatatta ctatgaaaca gttgattgta gatgcggcaa ataatggaga
     3781 aatgtatgct gtaaatatcc ctgctgtatc ttctgataat acaaaaacga tcgtttcaac
     3841 tattaaaggt gtttctaatc agttggaatt tatgagttca acttacaatt ctatgggcgt
     3901 tctcactcat acgcgcaaaa accgacaagt gcttattatt gatgctgccc ttgatgctgc
     3961 aattgatgtt gatgttttgg cttatgcctt taatatggat aaagccgaat tcatgggcag
     4021 aagagttctt gttgataatt ttggagaatt aacgggcgtt gttgcggctt tggtagatga
     4081 agactggttc atggtatatg ataatttcat tggtttcact gaaaactata atggccaggg
     4141 actttattgg aattattttt atcatgtttg gaaaaccttt tcgacttctc cgttctcaaa
     4201 cgctattttg tttacaacgc aggatgtagc agtcacaaat gttaccgtaa gtccgaacaa
     4261 tatcactcat aatactggtt cttctcaaac tgtgcagttt acggctaatg taacagccac
     4321 gggatatgcg cctaaagatg ttgtatggtc tactacttct gctactgcga caattactga
     4381 aaatggcttt cttacaattc ctacaactac agatacaacc tttactgtaa gagcaacaag
     4441 cgtttataat tctgcaaaat ttggtgaagc tacagtaaca gttaaataag gaggaaaaga
     4501 aaaaatggct gattttgtgc catcaacaat tgttaaagtg ttgaaagatg ttccattaga
     4561 ttctacatat agcgatacaa taaagtttac aagcgttgga gcacaaacag cctttttttc
     4621 tggtaaggct aaatattctt ttactgactt cacctatcag cgtgtaaatt cttctgtgtc
     4681 tgctcctagg gggccacgtt ctattcgtgt cccccgagtt gcagatgatc tatatgattg
     4741 caactatgtt atgtttcaaa atgcgaacta tggaacaaaa tggttttatg cttttataaa
     4801 acaagtaaat tatattaatc ctaacaatac agaaattatt tatgaattag atcattatca
     4861 aacatgggca tttgatttta cagtgttacc tagttttgtg gagagagaac atccagaaag
     4921 tgacaatttg tatgagaatt taattccaga aaacatgaac gaatcggata tgtatactca
     4981 acaggttaca gaactagatt tgcaaacgaa ttttgcaaaa atcgttgttg gagtatctac
     5041 agaccctggt ggccaaattg tagcaggaaa attaagcgga ggcgtataca gtggtgtgga
     5101 acttcatcaa tttgataacg cagagggagc aacagctttc attgaatcct atggaaaaaa
     5161 agctatggct gatgcgatag tttgtgttta tatgagcgcc tgggatgtaa atatatctga
     5221 tttaaagaat gttacaatat cccgccccac aaatttatgc ggatataccc ctagaaataa
     5281 aaaattattg acatatcctt attgtaaaat tacaatgagt aatagacaag gaactgaata
     5341 tgatttttat tatgaatatt tttcaagaat aggtaatcaa gaagtagaaa caacagatat
     5401 catatttaac tatcttactt acggaggaat tcaacctaaa ggatattgtg ttgctgtaaa
     5461 ttataacgga ttacagggcg atgtcaatat ggactataat gcagttggtg aagttgatgg
     5521 atacgttcaa tgtgcatgga cttcaaatgc ttttgcaaac tggttggctg gtgagggaac
     5581 agcacaagcc tttaaaatcg gcacaaatgc tttagcaact gcaaataaaa atacttttag
     5641 ttctggactt tctactatgc tttcagcctt tagtggaaat gtaggaggaa ctgtaagtgg
     5701 tgttcagagt atggcaactg gtacagtaaa caatttagta aatactgttc attctgctgc
     5761 cgatttagga attgacatat ggacaagaag taagtgtcct ggattatcaa aaggtacccc
     5821 acatacacct agtctgaatt taatttatag aaacattgga tttactataa aacaaatggc
     5881 aatcagaccc gaaatagcaa aatcaataga cgattatttt gatatgtatg gttatgctac
     5941 aaataaatta aaggttccga atatggaagg tcgtgaatca tggaattacg tcaaaacaaa
     6001 agatgtaatc atttcaggtt ctctccctgt agattctatg gacgctgtta agaaaatgtt
     6061 taattctgga attagattct ggcatggaga ttttgttggt cattattcaa gaagcaataa
     6121 gccaatgaag gatgtgaaaa aataatgagc agacgcaaac catataaatt gtttgacaga
     6181 tgggcgaata gaaaaaattg ggatccatat attttgaata atgaaacttt catggattac
     6241 ttttatagac tggaagaaat ttgcatcaat atgttttcat gggaaaactt acctgaaact
     6301 attgattatc gtttcttaga attaacatta tgcgagtatg gctttgctgt ttattttaat
     6361 gaagaagatg ttggaaatgt agcacttaca tgtgcattgg gaggcccact ctctatatat
     6421 agagaaccga tatatcggag agcatatgca aacaatgggt tttctcgcga attggataat
     6481 acaaacagtg ttttaattta caataattat ttgcatcatc cttctattcc aactataata
     6541 ttatttgcaa gacgattggc tgaaattgaa agaactatcg atgtaaacgt caaaagtcaa
     6601 aaaactcctg ttgtgattac ttgtgacgaa tctcagctat taacaataaa gaacgcctat
     6661 aaagatatgg atgaaaatgt tcctgttata attgcttcta aaaatgttga ctggaaaagt
     6721 atttcatcat tgaatacaaa tgctccattt gtatctttag atttgaataa tttaaaaaga
     6781 caaatatgga atgaagcatt aacatttttt ggggtttcta attcaaatac annnnnnnnn
     6841 nagaaagaca aataggatta gaagtttcat caaatttggg cggagttatg gcacaaagat
     6901 atataatgct aaattcaaga aggcaagcag cagaaaaaat aaacaaaatg tttggcacga
     6961 atataaaagt taattttagg caggaatttg aaactttcaa tgaagatatg gatactacga
     7021 ctatagatct aaatgaaagg gatgaatagg atttggcaaa atacaccatt gagttaaacg
     7081 aacttatttc attaggcttt catttgaatt tagatgatta tcccatattt aatgaagatt
     7141 acagggccca cctgaataaa gaaattatcg aacattttta ttttagagaa ataggccaag
     7201 aaactcctga aaggtttaat ttcttcttga agcgaaaaat ggccgaaatt atgccttatt
     7261 ataatcaact atatcaatct gaacttatta aatttgatcc cctttcaacc gtatacatgg
     7321 aaacctcagg agatactaaa aggaaaaaat ataatgagaa taccaatgat tataaacaaa
     7381 ataatacctt aaatgcaagt gagacatatg cctctaatat tgatcgttca aatacattta
     7441 aattagaaga tacacaagat accacaaata atagtaatta tcaaaagtca ggcgaccgaa
     7501 cacaggatat tgttactaac gaattaacta caaatgactt gaaaacagaa actaatactg
     7561 taagtgacag cgatggaaca accgatacac atggagttaa aaatactgta tttagtgata
     7621 ttccacaggc aggaataact actaccacta ccactgcacc agatggcaca atcactactg
     7681 aaacaacagg atatgctaca accacaacaa atgaatcaac taatgaacat agcacaacta
     7741 cagcccatca aactagtgat agtgtagcta ctaataccgg aactgttaat gttgacggaa
     7801 aatcgaattt aacagaaaat tggaaagaaa atggaagtgc tgtagatgtt ggaaattcta
     7861 aacaaaatca aaattcaaat gaagtcacaa acacaaaaga agattatcaa aaggctaata
     7921 ttttaaatga aaagactgac agaataaata gagaacatgg taaagaaaaa gaaaatatag
     7981 agaataccga atatagaaaa ggacggctag gtgtttcgcc atctgactta cttattaaat
     8041 atcgccaaac atttttgaat atagatatgc ttatcattaa tgatttggaa actttattta
     8101 tgggagtgtt ttaaatgaac aataacttta atggccatcc tcctaaacct gattgtcatt
     8161 gtaaaccaaa taaaccccat tgcggttttc ccgattttcc acagttttgt ccacctccgc
     8221 cgcctcctcc gccttgtgaa ccacaggtgc cttctgtagt agaagggcag tcattatatc
     8281 aggcagttaa taaccttacc aacagagtga acttgtgcat ccagaactat aatgatgtaa
     8341 tggctaactg ttatgctacg ctccataata tggagaaagc cgcagaagca aacggttcct
     8401 actacggccc ttgcgaagtc tggacagaag aagggtattc tgccgaacag ggttctacct
     8461 atacccttat ccataaggcc tgtgtagaca gacgtggcga gcctatcaga atgcaattac
     8521 atcttgccta tggaaatacc acgaactccc agattgagca gtctattttt agcgcatcca
     8581 aaattacact cgctgacaaa atggttgttg ctcagcctaa gggcgcaaat gggtggtacg
     8641 gtcacgcaat ttggcatggc gctcctatcg ctgcaggtag tgaacctacc ctttataccg
     8701 ttggttttac tcgccgtggg acgatgcgcg tctattccaa tgcagtttcc caagaccagc
     8761 ttctgaatga taccattgaa aacgcaatgg gctgctctgg tgttttgatt caaaatgggc
     8821 agatcaccga tgaatcctac caggctaata taccttcggc tactacgcaa actgcccgtg
     8881 ttgtaatggg ccagaatatg gatacccagg aagttatcat tctttgctgt ggatcctatg
     8941 ataacgttca gcataaggga atgacttcta aggcttgcgc tgaaattctc ctgaactatg
     9001 ggtgtgatat tgctgttgaa ttgtctgagg gtatttccgc cggagccgtt gataaaggct
     9061 ccatggtatt ccctcctatg aacaattctg ttcctacagc ttattgctat tggtatatat
     9121 ctcgtgcctg cctttataaa aacgattacc aaaaagaact cgctattcta atgcagaact
     9181 atggagaaac tctttgggga atctatgaaa gtacgcagaa aatcaatgat ctttcgaaca
     9241 ggcttgataa agaaattcaa gatcgaatcg atggagacaa tgcgttaaat gaggctttac
     9301 aggctgaaat agaaagagct caagctgaag aggctagact gcagggcctt attgaacaga
     9361 ttaataccca aatcacccag atcaaccaaa cgatatcgga aattcagtct gatatctcag
     9421 atattaataa agaaattacc gatatcaaag gtgacattac taccattaat gagcagatct
     9481 tgcaattgtc taatcgactt acagcgctga cagcttctgt tacagccctc cagcagactg
     9541 tacagtcaat tcaagaggac attaccaatc tagaattggc tgtcaatacg ttgtctgcta
     9601 ctgtgaccaa tatcatcaat ggaacagaag tgcttcccta tctttctatt aatggcggta
     9661 ctatgaatgg tgatattgat atggctaaca ataccataac caatgtaccc actcctacgg
     9721 caaatgctga cgctgttaat aaaaagtatg tagatgatgc tatttctgga agcattactc
     9781 cgcctacggg agattatctg ccccttactg gcggtgaaat gcatggcgct atcgctatgg
     9841 gtgactttcg tattacaaac ttgggcaatg ctacttccaa tggtgatgct atcaataagg
     9901 gacaagcaga taccctctat attagaaaag ctggcgatac tacccctgga gcacaaacgt
     9961 ttcagaatac tatcaccatt ggtagttcta ccgatggcat ggtaatggat cctgaaagtg
    10021 gttcccctaa cttgtctata gcggcaggta atgaggcaat tcatttggga gtaacgtctg
    10081 aaaaagcttt tattaacagt gagtctaacg agatcgactt tactgcagga agtgcagggt
    10141 cacctgaata tacttcttta agaggtatcg cagagcctac agaagactat gatgctgcta
    10201 ataaaaaata tgtcgatcaa gcagtttcag gagctgggga aggctctttt cttaaaaaag
    10261 gtggagatac aacaactggg aaatatagct ttacatcttc taattcgacc ctcactgttg
    10321 gccccgaagc aatccaaaat acttcaagtg ttgcccctgg caaaattgta ttaaatggtt
    10381 cgtctagtaa atctattgaa ttaaattcaa atgcactaag cccaaatatc accttgacaa
    10441 atgctggaca ttctgctaaa ataatagcag gagcagatag cacaaatttt attgattcaa
    10501 gtaataagtt ctcttttgca agcgggtcta gtactgctat attggatggt atcgccaatc
    10561 ctacagctga ttctcaagct gtccctagag gatatcttaa ttctcaaatg agtgacaagc
    10621 taagtattcc aacaaatggg tggggattta cagccgcttg tagatataac gataaaatta
    10681 tttatgccac aggtgataaa gcatatggtg ttgcttctgg aaatgatttg gctatggtaa
    10741 cagatattag ta
//
LOCUS       DBMDWL010000065        10728 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2132_length_10728_cov_2.516232, whole genome shotgun sequence.
ACCESSION   DBMDWL010000065 DBMDWL010000000
VERSION     DBMDWL010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2132_length_10728_cov_2.516232"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            121..297
                     /locus_tag="ACZPDN_05815"
     CDS             121..297
                     /locus_tag="ACZPDN_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298172.1"
                     /translation="MWGLCSEGLTGEQEFIRTLMDDEIVEEILWNFTPYDFEDLLVQY
                     KALRKIQRRIYILN"
     gene            complement(324..1019)
                     /locus_tag="ACZPDN_05820"
     CDS             complement(324..1019)
                     /locus_tag="ACZPDN_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298173.1"
                     /translation="MASTNKTVTLELSQFIATDKPSWLTDYNGDMGKIDNFAAGVDAD
                     ISSAKSDAASAKSTAQTANNTAQQAQTSANQAQGTANTAQSTANTALTNAEQALQTAN
                     MLADGWKTATITNPDTTNMTLYYQACRYNKSAGLLYINASLSVSNKSIGENTVILNIP
                     NVIAGANKEIISGLLRIHTNEDKSYNINGTIDTTGNILYGPQAATTTKITSISICACI
                     SMAGEGNGWVAIS"
     gene            complement(1043..1834)
                     /locus_tag="ACZPDN_05825"
     CDS             complement(1043..1834)
                     /locus_tag="ACZPDN_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298174.1"
                     /translation="MSIPYVRNMLCCVNPTLPSSYQATFSWYEDLLALQKAINEIITW
                     VNNYEGVTDDWVKEYVASQLKGISDEITQFEESVNARLEENEQKYIDFTQEITNKVND
                     ILLEVDEKNKAFYAIIIAKVKEMLDEVINKLSEQTPVTNPFTGFYDSLKNVLYMLYDG
                     VRADGITAYNYDCQKIEAQRYDGFRLTAFDYDTAFLEKCHNVAYRVFSSFTGLKTSLQ
                     QAFSEIYQALRTGGITATEYDEKDLTAEAYDAKAITAFNYSWNYN"
     gene            complement(1851..2474)
                     /locus_tag="ACZPDN_05830"
     CDS             complement(1851..2474)
                     /locus_tag="ACZPDN_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298175.1"
                     /translation="MSNYTTQLRYIIGAGIDIGLKDYPIFDETYRETLNRKLIEHYWM
                     DEIGSETIQLFVFRLNRNMREIMPYYNELYKSAQLKFNPLEGYNMTEILSETEKNNSN
                     ATANGTSSGNGKNLFNDTPQNAIGQGDILSQKYATNITLDETETNTTSNTNSNSQRGR
                     EEMRTTKGSQYHNPSELLQSYRETILNIDMMIIEDEKIRNCFMCLLA"
     gene            complement(2467..3354)
                     /locus_tag="ACZPDN_05835"
     CDS             complement(2467..3354)
                     /locus_tag="ACZPDN_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017190.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298176.1"
                     /translation="MKHGIGRDVFGELRNSLQLNNGAFNEYFLKLMDMLLVRFRYNNV
                     PDSLDIRYLELKLITEGSVIVFKDEYAGLMGLGTSYQGVLDQYGLPSERQAIAANGVP
                     FLNLNETNSVMIFNNRMRAPDIHLVLLYAQRLWDIQRSIETNCKLQKFSGVAITTQDK
                     LLSMKNALNKWDGNQPFILATKDFDVDAFKGMGFEIPFVADKLLTIKRELFSEALSNF
                     GIINSANEKRERMNVMETASPFGHVEMARNSYLTARQEGIDWVNKMFGTNITVEFNSN
                     IPIVLDNDNLTEGNGGEYE"
     gene            complement(3367..4986)
                     /locus_tag="ACZPDN_05840"
     CDS             complement(3367..4986)
                     /locus_tag="ACZPDN_05840"
                     /inference="COORDINATES: protein motif:HMM:NF044352.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298177.1"
                     /translation="MNITPQTVIYLCAGVPFDNTYEHVRLLETTSDKINYFSQFVISH
                     MSGCMYQRQTSVINYMADYDSIASANYLYFRNIDDGKYYFAFIRSIEFVNQNKSSITF
                     EIDAFQTWFDKNSLRECFVEREHVNVDTFGKHLVPENLETGEYINNRDMFIPAIVTNL
                     TPCIIMGVSEVLNGGALGEIIDFTYSGLAYFWCSIDRPADVTNLIKQYAENGKANAIV
                     NMFMYPIELLGKDSAASGSGWLSNFPTKPVTDFFITNLFAPLDGYTPKNNKVYTSPYR
                     YFVIYGPGSMEKAYFYEYFSEFENMFHIFSTFGGSAPIVAVPQNYKGYVSKSDEAIQI
                     YPYPTCSWVNDNYANWLAQNQVSMNYQIISGGLSSILNIVGSGITAAAGVAAAPVTGG
                     ASLIATASGVSGIVGSTKSGVDNVANVLIKNYQAQMVPDSARGSTAVANSFYASGDHY
                     LHATPMCIRYEYAKRIDEFFTHYGYKVSDFKIPNITGRKSWNFVKLVDCNINLTAPVE
                     IVEKIKSVFLSGVTFWHTDDVKNYSLDNSIV"
     gene            complement(5076..6383)
                     /locus_tag="ACZPDN_05845"
     CDS             complement(5076..6383)
                     /locus_tag="ACZPDN_05845"
                     /inference="COORDINATES: protein motif:HMM:NF052097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR1298178.1"
                     /translation="MKTMNTVKAMPKNTVDVLKAINNEMLRNNPGFEDRLPEVTQDNI
                     REYGTALLSYQPTQNEFIDTMVNLIGRIWIEYRMFTNPLRVLKKGMLEYGDTVEMMYT
                     RIAKAHQFDPEQAETEWMKREIPDVVTAFAKRDYTVFYKATISDDMLRAAFMGWGQLS
                     DFINSVFNSMYTGAEFDEYRTMMQLLDNYGAAGKFGILTVNDITDKDSAHEALAKVKA
                     LSNKMTFLRNDYNSLGVVTATPKANQVLIIDADWDAYFDVQGYATLFNLEPARAQYRK
                     IVVDDFPTTLKNGGVHAILIDEDFYACWDCLQKFVRDMNGQGLYWQYWAHYWRILAVC
                     PFANAVAITTETPTITAVDITPKTPTVNKGSSIQFTATITGTGLFPQNVTWSVMGNSD
                     PTTTITPMGMFTMGANETGAVQVKATSVFDADKNSSTTVTVGS"
     gene            complement(6380..6763)
                     /locus_tag="ACZPDN_05850"
     CDS             complement(6380..6763)
                     /locus_tag="ACZPDN_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298179.1"
                     /translation="MTREEEKELLDKILSDERLTDDMKEDLRKLKDSYNEQKGAEDEK
                     SEEVKEHDVDEWEQLVNERDNAYKERDEAISKFKDIKEKYIRRIITPEKAMEMHEKDY
                     NSDDFEDIPGKTKKEEIFEEEEIVK"
     gene            complement(6753..7853)
                     /locus_tag="ACZPDN_05855"
     CDS             complement(6753..7853)
                     /locus_tag="ACZPDN_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298180.1"
                     /translation="MAQKVLFPGTHMRITQDEYGKTSHVGSLARDDGSESTAFNSPVL
                     APFDGYFARVRADSSHETYFVSEGPVECANGYVGVVTFLFMHDNVTRFPVGIHKKQGE
                     IIGYEGGFKDGRKDAVAHHTHREWSRGKNITQYRNSNGTFVIANQMHEYDVCFLRPET
                     WVYTGSKFKVAKDFNGIIKDNVGHTFIMSEEGETVDVKPIDNENNIVLQCGPASSGDR
                     YYLKQVAENKGLGYSESNPVSGDNKYVYIGPASPGDQIVVISKCNELGLPCVKYVEPE
                     PEPEPEPEPEPEPEPEPEPEPEPEPEPEPSKEVEELKEEIKRLEAANKSLAAINKDLE
                     NRLEKKEAEANNYYNALVDINNILKGVKENDT"
     gene            complement(7853..8056)
                     /locus_tag="ACZPDN_05860"
     CDS             complement(7853..8056)
                     /locus_tag="ACZPDN_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298181.1"
                     /translation="MDIVNAVTSVIGSLGFPIAVCCWLLYDKTQERKAHTEEMAEMRK
                     AIESNTSVMSELKTMIATLHGVK"
     gene            complement(8071..8787)
                     /locus_tag="ACZPDN_05865"
     CDS             complement(8071..8787)
                     /locus_tag="ACZPDN_05865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1298182.1"
                     /translation="MSYSTYMFDYSQMQVTAGYPAYPDGRPHRGIDIKTPNNAYRNLG
                     SPVSGEVIRSEYGTGGNWTWGNFICVYDSSRDVTILMAHFAERLVSVGATVNAGDIIG
                     VYGSTGNVTGPHCHVEQHAGRGITNVLQDPSGIIGVPNAIGTYDIEYAGGETPPTPPG
                     SLTQNILLVVFNYNGHTINAPASNDEKYIYFNNENFYRFPIGDYDKVQQYGSWKYWQD
                     ITNVNILAVFNKDLGSLPDV"
     gene            complement(8796..9788)
                     /locus_tag="ACZPDN_05870"
     CDS             complement(8796..9788)
                     /locus_tag="ACZPDN_05870"
                     /inference="COORDINATES: protein motif:HMM:NF017687.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA encapsidation protein"
                     /protein_id="HIR1298183.1"
                     /translation="MYYDIDRACSYNEPFNMILGGRGIGKTYSWKKKAIRAFLKNGIQ
                     FAYIRRYDSELQQVADTLFNDIIFNEEFPGHDIKYEKGVWKVDKEVAGFPFALTRNKD
                     YKSASYPRINNLLFDEFLVENGRSAYLKREPFKLFDLYETIARMRDNVVLFMMANTIS
                     KANPYFLEWNLMPQKDKEFIVRNHILLQIVPVDNEFRETKNSTRFGEMSRALGYADYS
                     VDNNFYVDTDVQTFSKTKSSRYMFSFVWKGKTYGFWFDYKSGYNIISYDYDSYSTRIF
                     TLDKESVSATLNYINEYSKHPYFAKIRQSFCNGTLRYEHEKIQHEIKDMLVLLL"
     gene            complement(9959..>10728)
                     /locus_tag="ACZPDN_05875"
     CDS             complement(9959..>10728)
                     /locus_tag="ACZPDN_05875"
                     /inference="COORDINATES: protein motif:HMM:NF015154.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR1298184.1"
                     /translation="SSGDDAIDLCLTSVDYELFREHYNLYNVEYYGGWKFKQSNKLFR
                     EYIDKWMAIKVKASKEGNKTMRTWAKIMLNSLYGKFALNPICARKSPYIGEDKRMHFK
                     TLEKEEREPLYLPVGAFITAYARKFTIETSQEVRDDSLKKYGKDMYIYSDTDSIHTLL
                     PYDDIIKIIDVDDNALGKWKHESEFTRARFIRAKTYIEEIEGKMCITCAGLPERCYKE
                     VTWENFHPGVTYYGKLLPKQEIGGIVLHETEFHIHAT"
BASE COUNT         3039 a         2351 c         1788 g         3550 t
ORIGIN      
        1 cagtgaaagg gacagtgaac attctgaacc gagtagactt gacctagcat tccaagaggg
       61 aagcagaggc cgggcgtcca ctttacaaac cgcctaaaat ggataaggag atggagagat
      121 atgtggggtt tgtgtagtga aggattaaca ggcgagcagg aatttattcg cactttaatg
      181 gatgacgaaa ttgttgaaga aattttatgg aatttcacac catatgattt tgaagattta
      241 ctggtacaat ataaagcatt acgcaaaata caaagaagaa tttacatatt aaattaagcc
      301 cctcgaattg aggggcttaa tttttatgaa attgctaccc agccattacc ttcaccagcc
      361 attgaaatgc atgcacatat cgaaatggac gttatttttg tagttgtagc ggcctgtggg
      421 ccgtacagga tatttccggt tgtgtcaata gttccattta tattatatga tttatcctca
      481 ttagtatgaa ttcggagaag cccgctaata atttctttgt tagcgccagc tataacattc
      541 ggaatattaa gaattaccgt attttcgccg attgatttat ttgatacgga aagagatgca
      601 ttgatataca ataggccagc acttttatta taacggcaag cttgataata aagtgtcata
      661 ttagtagtat ccgggttagt aatagtagca gttttccaac cgtctgcaag catattagcg
      721 gtctgaaggg cctgttccgc atttgtcaag gcggtatttg ctgtgctctg cgcggtgttt
      781 gccgtacctt gagcctgatt agcggaagtt tgagcctgct gcgccgtatt gtttgccgtc
      841 tgtgccgtag attttgcact agctgcatca ctttttgcac tggaaatatc tgcatccacg
      901 ccagccgcaa agttgtcaat ttttcccatg tcaccgttat agtccgtcag ccatgacggt
      961 ttatcggttg caataaactg tgaaagctca agtgtaacag ttttgtttgt agaggccata
     1021 atagattcat cctttcaaaa ttttagttat aattccagct ataattaaaa gctgtaattg
     1081 ctttagcatc atatgcttct gcggttaaat ctttttcgtc atattctgtt gctgttatac
     1141 cgccagtacg taaagcctga tatatctcgg aaaatgcctg ttgcaatgat gttttcaagc
     1201 ctgtaaagct gctgaacact ctatatgcaa cattatggca tttttccaga aatgctgtat
     1261 cataatcaaa agcagttaaa cgaaatccat cataacgttg tgcttcgatt ttttgacagt
     1321 cataattata ggccgtaata ccatcagcgc gaacaccatc ataaagcata taaagcacat
     1381 ttttcaaact atcataaaag ccagtaaagg ggttagttac aggggtctgt tcagaaagtt
     1441 tatttataac ttcgtccagc atttctttta cttttgcaat tataatagca taaaaagcct
     1501 tatttttttc atcaacttct aaaagaatat cattaacctt gttagttatt tcctgtgtga
     1561 agtcaatata tttctgttcg ttttcttcta atcgggcatt tacactttcc tcaaactgtg
     1621 ttatttcgtc acttatgcct tttaattgag atgcaacgta ttctttaacc cagtcatcag
     1681 ttacgccttc atagttattt acccatgtaa taatttcatt gatagctttt tgcaaagcca
     1741 gtaaatcctc ataccatgaa aaggttgcct ggtaagagct tggcaatgtc ggattaacac
     1801 agcacagcat atttcttacg taggggatgc tcatttttct cactcctttc ttacgccaat
     1861 aggcacataa agcaatttct tattttttca tcctcaatta tcatcatatc tatattaagt
     1921 atcgtttcac gatacgattg caacaattcg cttgggttat gatattgtga gccttttgta
     1981 gtacgcattt cttcgcgtcc gcgttgactg ttagagtttg tattactggt tgtatttgtt
     2041 tccgtttcat ccagcgtaat gtttgtagca tatttttgtg ataatatatc accttgtcct
     2101 attgcatttt gcggtgtatc attaaacaaa ttttttccgt ttccactaga tgttccatta
     2161 gctgttgcat ttgaattgtt tttctccgtt tcgcttaaaa tttcggtcat gttataacct
     2221 tccaagggat taaatttaag ctgtgcactt ttatacaatt cattgtagta gggcattatt
     2281 tcgcgcatgt ttctgttaag acgaaataca aacaactgaa ttgtttcgct tccaatttca
     2341 tccatccaat aatgttctat tagtttccgg ttaagcgttt cacggtatgt ttcatcaaaa
     2401 attgggtaat ctttaagccc tatgtcaata ccggccccta tgatatagcg tagttgtgtg
     2461 gtgtagttac tcatattcac cgccatttcc ttccgttagg ttgtcattat ccagtacaat
     2521 cgggatgttg gagttaaact ccactgtgat atttgtgcca aacattttat ttacccagtc
     2581 gataccctct tgtcgggcgg tcaagtaaga gttgcgtgcc atttcgacat gcccaaaagg
     2641 cgaggcagtt tccatgacat tcattctttc acgcttttcg tttgccgaat ttataatccc
     2701 aaaattagag agagcctctg aaaataattc gcgcttgata gttaatagct tatcagcgac
     2761 aaacggtatt tcaaagccca tccccttaaa tgcgtcaacg tcaaaatctt ttgtcgccag
     2821 aataaaaggc tggtttccat cccatttatt tagagcattc ttcattgaca aaagtttgtc
     2881 ttgtgtagta atggcaaccc cactgaattt ttgaagcttg caatttgtct caatactgcg
     2941 ctgaatatcc cataaccttt gggcatataa aagcactaaa tgaatatcag gcgcacgcat
     3001 acggttatta aaaatcatta cgctgtttgt ttcgttaaga tttaggaatg gaaccccgtt
     3061 cgccgcgatt gcttgtcgtt cggaaggtag gccatactgg tctagtacgc cttgataact
     3121 tgttcctaat cccataagcc cggcatattc atccttaaaa actattacac tgccctctgt
     3181 tattagttta agttccaaat aacgtatatc tagactatca ggcacattat tataccgaaa
     3241 acgcactagt aacatatcca ttaactttag aaaatactca ttaaatgctc cattattaag
     3301 ctgcaaagaa tttctaagtt ccccgaaaac atcccttcct attccatgct tcatttcaac
     3361 acccccttat acgattgaat tatccaatga ataatttttt acatcatctg tatgccagaa
     3421 tgtaacgccg ctaagaaata cgcttttaat tttttccaca atttcaaccg gggctgtcaa
     3481 attgatattg cagtctacaa gttttacaaa attccagctt ttacgcccgg ttatattcgg
     3541 aatcttgaaa tcagaaactt tataaccata atgtgtaaaa aattcgtcaa ttctttttgc
     3601 atattcgtat cttatacaca taggtgtagc atgtaaatag tggtcgccag aagcataaaa
     3661 agaattggca actgcggttg aacctcttgc actatccggc accatttgtg cctgataatt
     3721 tttaattaac acatttgcaa cattatcaac accgcttttt gtgctcccga ctataccgct
     3781 tacaccactt gcagtagcta tcaaagatgc cccaccagta accggggccg ctgcaacacc
     3841 agccgcggca gttattccac ttccaacaat attcaaaatg ctgcttaaac caccactaat
     3901 tatttgataa ttcattgata cctgattttg agcaagccag tttgcataat tatcattcac
     3961 ccacgaacaa gtgggatagg gataaatttg tattgcttca tcagattttg aaacataacc
     4021 tttatagttt tgggggactg ccacaatagg tgccgaaccg ccgaaagtag aaaaaatatg
     4081 aaacatattt tcaaattcag aaaaatattc ataaaaatat gctttttcca ttgagccagg
     4141 gccgtatatt acaaaataac gataagggga agtataaacc ttattatttt taggggtata
     4201 gccatctaaa ggcgcaaaaa gattagtaat aaaaaagtct gttactggtt tagtggggaa
     4261 attagataac cacccgcttc cagatgcagc actatctttt ccaagcaatt caatcggata
     4321 catgaacatg tttacaatag catttgcttt tccattttct gcatattgct ttattaaatt
     4381 tgttacatca gcgggccggt caatcgaaca ccaaaaataa gcaagtcctg aataagtgaa
     4441 atcgattatt tcgcctagcg ctccaccatt cagcacttca cttacaccca taattataca
     4501 aggtgttaaa ttagtcacaa tcgccgggat aaacatgtct ctattattaa tatattcgcc
     4561 tgtttccaag ttttcgggga ctaaatgttt accaaaagta tcgacattta catgttcacg
     4621 ttctacaaaa cactccctaa gggaattttt atcaaaccaa gtctggaaag catcaatttc
     4681 aaaagtaatt gagcttttat tctggtttac aaactcaata gagcgaataa acgcaaagta
     4741 atatttacca tcatcaatat ttctgaagta gagataattt gcgcttgcaa tactatcata
     4801 atcagccatg taatttatta cactggtttg acgctgatac atacaccccg acatatgaga
     4861 tattacaaat tgactaaaat agttaatttt gtcgcttgtg gtttcaagca atctcacatg
     4921 ttcataagta ttgtcaaacg gaacacccgc gcataaatat atgactgttt gaggagtaat
     4981 attcatttct ttctccttaa aatgagggtc gggatttatg aatgaaacta ttaaacattt
     5041 ttgagcttca agcctactct cccgaccccg ttaaattaag aaccgactgt cacagtcgtg
     5101 gagctatttt tatcagcatc aaatacactt gtcgctttca cctgtacagc gccagtctca
     5161 tttgcaccca tggtaaacat acccataggg gtgatggtag tagtcgggtc actgttaccc
     5221 ataacagacc aagtaacatt ttggggaaaa aggcctgttc cggttatagt agcggtaaac
     5281 tggatagagc tacccttatt aacagtaggg gttttcggtg taatatcaac cgccgtaata
     5341 gtgggcgttt ccgtggtaat tgcaacggca ttagcaaacg gacacaccgc caaaatacgc
     5401 cagtagtgcg cccagtactg ccaatagagg ccctgcccgt tcatgtcacg gacaaatttc
     5461 tgtaagcaat cccagcatgc ataaaaatcc tcgtcaatca aaattgcatg tacgccacca
     5521 tttttaagcg tagtggggaa atcgtctaca acaattttac gatactgcgc gcgagcaggc
     5581 tcaagattaa acaaagttgc atatccctgg acatcaaaat aagcgtccca gtctgcatcg
     5641 ataattaaca cctgattagc tttcggggtt gcagtaacta ctccaagaga gttgtaatca
     5701 ttccgcaaaa acgtcatttt gttggaaaga gctttaactt ttgcaagagc ttcatgcgcg
     5761 ctatccttat cggtaatatc atttacagtc aaaatgccaa acttaccggc cgcaccataa
     5821 ttatcaagca actgcatcat agtacgatat tcatcaaatt ctgccccggt atacatggag
     5881 ttaaagacag aattgataaa atcggaaagc tggccccatc ccataaaggc cgcgcggagc
     5941 atatcgtcgg aaatagttgc tttataaaat acagtataat cacgttttgc aaaggcggtc
     6001 acaacgtccg ggatttcgcg tttcatccat tctgtttcgg cctgctccgg gtcaaactga
     6061 tgggcctttg caatacgggt atacatcatt tcaaccgtgt cgccatattc aagcatcccc
     6121 tttttcaaca ctcggagggg gttggtaaac atccggtact caatccagat acgcccgata
     6181 agatttacca tggtgtcgat aaattcattc tgcgtaggct ggtacgacaa aagggctgtg
     6241 ccgtattctc gaatattatc ctgcgtaact tccggcaacc tatcttcaaa gccggggttg
     6301 ttgcgaagca tctcgttatt gattgctttc agcacatcaa cagtgttttt aggcatagcc
     6361 ttaacagtat tcatagtttt cattttacaa tctcctcttc ttcaaaaatt tcttcttttt
     6421 tagttttacc gggaatatct tcaaaatcgt cgctattata atctttttcg tgcatttcca
     6481 ttgctttttc cggtgtaata atacgccgaa tatatttttc tttgatgtcc ttaaatttgc
     6541 taattgcttc atcacgctct ttataagcgt tatcacgttc attcacaagc tgttcccatt
     6601 cgtcaacatc atgttctttt acttcttccg atttttcatc ctctgcgccc ttctgctcgt
     6661 tataactatc tttgagtttt cgcaagtcct ctttcatatc atccgtaagc cgttcatcac
     6721 tcaaaatttt atcaagcaac tctttttctt cttcacgtgt cattttcttt tactcctttc
     6781 aaaatattat ttatatcgac taaagcgtta tagtaattgt ttgcttcagc ttcttttttc
     6841 tcaagtctgt tttctaaatc tttattgatt gcggcaagtg atttatttgc agcttcaagg
     6901 cgttttattt cttcctttaa ttcttcaact tctttagatg gttccggttc cggctctggt
     6961 tccggctccg gttccggttc cggctccggt tccggttccg gctctggttc cggctccggt
     7021 tccggttccg gctccacata ttttacacaa ggcaacccta attcattgca tttactgata
     7081 acgactattt ggtcacccgg tgatgccggg ccaatatata catatttatt atcgcccgaa
     7141 actggattac tttcggaata tcctaatccc ttgttttcgg caacctgttt caaataatac
     7201 ctgtcaccgg atgatgctgg cccgcattgc aatacaatgt tattttcgtt atcaatcggc
     7261 tttacatcca ctgtttcacc ttcttcgctc attataaatg tatgaccaac attatcttta
     7321 atgattccgt taaaatcttt agctacctta aatttactcc ctgtataaac ccatgtttcg
     7381 ggccgcaaaa aacatacgtc atattcatgc atctggtttg caatcacgaa cgtaccgtta
     7441 ctattcctgt actgtgttat gttcttcccc cggctccatt cgcgatgcgt atgatgcgca
     7501 acggcatctt tacgcccatc cttaaagcca ccctcataac cgataatttc cccctgcttt
     7561 ttatggattc caaccggaaa acgggtgacg ttatcatgca taaaaagaaa tgtaacaaca
     7621 cccacatagc cgtttgcaca ttccaccggc ccttcgctca caaagtacgt ctcgtggcta
     7681 ctgtctgcgc gcacacgggc aaaatatcca tcaaagggag ctaacacggg actgttaaag
     7741 gccgtgctct cgctaccatc atctcttgca aggctcccca catgagatgt tttaccatat
     7801 tcatcttgcg taatacgcat atgcgttccc ggaaataata ctttctgtgc cattacttta
     7861 ccccgtgcaa agttgcaatc attgtcttta attccgacat tacagacgta ttgctttcaa
     7921 tagcctttcg catttcagcc atttcttctg tatgtgcttt gcgctcctgt gttttatcat
     7981 acaagagcca acaacatacg gcaatgggga aaccaagact gccaataacg ctagttactg
     8041 catttacaat atccatttta ctccacccct tcatacatcc ggcaaactgc ctaaatcttt
     8101 attaaatacg gcaagtatat ttacattggt tatatcctgc caatatttcc atgacccata
     8161 ttgctgtact ttgtcataat ctccaatcgg aaaccgataa aaattctcat tattaaaata
     8221 aatatatttt tcatcatttg aagcgggtgc gtttattgtg tgtccattgt agttaaatac
     8281 cactaacaaa atattttgcg ttaaacttcc cggtggtgta gggggagttt ccccgcctgc
     8341 atactctata tcataagttc ctatggcgtt tgggacgcca ataattcctg acgggtcttg
     8401 taaaacgttt gtaattcccc ggccagcatg ttgttctaca tgacagtgtg ggccggttac
     8461 attccctgtg ctcccgtaaa caccaataat atcacctgca tttacagtag cccccacaga
     8521 tactaaacgt tctgcaaaat gggccatcaa aatggtaaca tcacggcttg aatcatacac
     8581 gcaaataaag ttgccccacg tccaattccc acctgtccca tattctgaac ggataacttc
     8641 ccccgaaacc ggactgccta aattgcgata agcattatta ggagttttta tgtcaatccc
     8701 tctatgcggt ctaccatctg gatatgctgg ataacctgcc gtcacctgca tttgagagta
     8761 atcaaacata tatgtactat acgacatata caacctcaca acaataatac cagcatatct
     8821 tttatttcgt gctgtatttt ttcatgctca taacgtaaag ttccattgca aaaactttgc
     8881 cttatttttg caaaatacgg atgcttgcta tactcattta tatagtttag cgttgcgctt
     8941 acactttctt tgtcaagcgt aaaaattcgg gtagaatagc tatcataatc atatgagatt
     9001 atgttataac cagatttata atcaaaccaa aaaccatatg ttttaccctt ccaaacaaaa
     9061 gaaaacatgt aacggcttga ctttgttttt gagaaagttt gtacatcggt atctacataa
     9121 aaattattat ctaccgagta atcagcataa cccagtgccc ggctcatttc tccaaaccga
     9181 gtagaatttt tagtttctct aaattcattg tcgactggaa ctatttgcaa taaaatatga
     9241 tttctcacaa taaattcttt atctttttga ggcatcagat tccattccaa aaaataagga
     9301 ttagctttac ttattgtgtt tgccatcata aacaaaacta cgttatccct cattctcgca
     9361 atagtttcat ataaatcaaa aagcttaaaa ggttcccttt taagataggc cgaacgccca
     9421 ttttcgacta aaaattcatc aaataacaag ttatttattc taggataaga tgctgattta
     9481 taatctttgt ttctggttaa tgcaaaaggg aaacctgcaa cttctttatc aaccttccat
     9541 acaccctttt catatttaat atcatgtccc ggaaattcct cgttgaaaat tatatcatta
     9601 aataatgtat ctgctacttg ttgtaattca ctgtcatatc tgcggatata agcaaactgt
     9661 atgccgtttt tcaagaaagc tcttattgcc tttttcttcc atgagtaggt tttaccgata
     9721 ccacgtccgc ctaatatcat gttaaacggc tcattatagc tgcatgccct gtctatatca
     9781 taatacatat ctatcaccct caataggcaa gataaacagt tcacaaaaat agcttgcaag
     9841 gccctttcgt ccggtacagc ggtttttacg ccgtgcatcc tgtccggaat agttgcgttt
     9901 aactgtttat cctacctatc ctcattatat tctttttacc taattataaa ccatgtaatt
     9961 atgttgcatg aatatggaat tctgtttcat gcaggactat cccacctatt tcctgctttg
    10021 gtaatagttt accataatat gtaacccccg gatggaaatt ttcccatgta acctctttat
    10081 aacagcgttc cggcaagcca gcacaagtta tacacatttt accctctatt tcctcaatgt
    10141 atgtctttgc ccttataaat cttgcccttg taaattcact ttcatgtttc cactttccta
    10201 acgcattatc atctacatct attattttta ttatatcatc atatggcaat aacgtatgaa
    10261 tactgtcggt atcggagtaa atatacatat cttttccata tttctttaag ctgtcatccc
    10321 gcacttcctg tgacgtttca atagtaaatt tccttgcata tgcagttata aatgcaccca
    10381 ccggcaaata taagggttcc ctttcttctt tttccaatgt cttaaagtgc atccgtttat
    10441 cttctccaat atacggggat tttctggcgc atatcggatt taatgcaaat tttccataca
    10501 aactatttaa cattattttt gcccatgtgc gcatagtttt atttccctct ttacttgctt
    10561 ttacttttat cgccatccat ttatctatat attccctaaa caatttattg ctttgcttaa
    10621 atttccaacc gccataatat tctacattat acaaattata gtgttcccta aaaagctcat
    10681 aatctacact tgttaaacat aaatctatgg catcatctcc actacttt
//
LOCUS       DBMDWL010000066        10620 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2171_length_10620_cov_2.702063, whole genome shotgun sequence.
ACCESSION   DBMDWL010000066 DBMDWL010000000
VERSION     DBMDWL010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_10620_cov_2.702063"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="ACZPDN_05880"
     CDS             complement(<1..212)
                     /locus_tag="ACZPDN_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298185.1"
                     /translation="MSADDYINSQVDPCIRRYEDLFRKYHKIHMGLMIACFSCFIGVI
                     ILNVVYIIVPVIWLVLASIVCTLGGL"
     gene            complement(214..1701)
                     /locus_tag="ACZPDN_05885"
     CDS             complement(214..1701)
                     /locus_tag="ACZPDN_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1298186.1"
                     /translation="MKSKFSKVLSILLSVVLVISSFYLLYQVIRLNVLPSKFLFPLTI
                     GVVVLDAIFILLLIYFSKNVVSKIVCIILTLFICVASCMGGYYISKTQNVLSSITNVA
                     KHAKNTVSVVVKESSSIKNKSQLNGVSVGSLRLNEQGSKKALKELSSEGIVLNQTEYD
                     SMTTLLEAFYNGEVDSIIINESSRSQILDMEAYSNFDSNTRVVYQTSYKVKNNDSATS
                     VSDITSKPFNVLISGSDTRGGFDENGRSDVIMIATVNPKTHTILLTSVPRDFYVTTAC
                     DAGDGCMQGALDKITHTGIHGTNTTKRTVEQLLGIEINYTFKVGFDTVTELVDVLGGV
                     DVYVEPGYATNSFAALSGFSVPEGVNHLNGEQALAFARERYSYTEGDRQRTKNQQQVL
                     MGIVKEATKPSVITNYAAIMDTMANTFSTTMSNEEITDLIKYQLNNNPTWKMEQYMVD
                     GTGDTLMCAELGDAASVMVPDQSTVKMAKDKINAVLAGKSSEDVK"
     gene            complement(1704..2987)
                     /locus_tag="ACZPDN_05890"
     CDS             complement(1704..2987)
                     /locus_tag="ACZPDN_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HIR1298187.1"
                     /translation="MRFYGEYTIKLCALYFLMQLLIGRYQGKVLLMYDEIRLLILSHL
                     GFFFGSFLIFPFSSWTIRKIYGFLLLTLVLFLFAGCNSRYSHKIFRQRYKHNTLIVGT
                     GHTAAQLAFVCRGNSYSLLDVQGYVSCKSYGIHQEQVVSKKQTIDLDAVDDFIRTHNI
                     DTVLIAIPEINRKNLNRLFELFKGKVLNIKYLPQVTGLVTFDTKVEDFDGILMISNSR
                     GVNQLGSYILKRCMDICAGLCGMMILIPLTVYVFFKNRMEGDRGPVFFTQNRIGKNGK
                     EFKMYKYRTMVLGADKILEDLMASDPKIREEYQKNKKLSNDPRITSAGHFLREKSLDE
                     FPQFINVFFGQMSLIGPRPYLPKEKVDMGEYYEDVVACKPGITGMWQSHGRSDVSFEQ
                     RLILDEYYYRNWSFWLDVTLLCKTVKQVLYGRGAV"
     gene            3428..3958
                     /locus_tag="ACZPDN_05895"
     CDS             3428..3958
                     /locus_tag="ACZPDN_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc ribbon domain-containing
                     protein"
                     /protein_id="HIR1298188.1"
                     /translation="MQIIICGEMSKRIHWNNRGCKSVVWRCFSRLESTGLESHARTIN
                     EPVLQDTVVKAINQMLGDKSSYQAQQQLNIASVIRASQATSVENIDEKLMALQQELIQ
                     KAQSKEVYDEIADDIFRLRELRQQTTIENAARDKQIKRINDLQDYIAQQTTRLTAFDE
                     SLVRRWLKQITVWMTT"
     gene            complement(4065..5696)
                     /locus_tag="ACZPDN_05900"
     CDS             complement(4065..5696)
                     /locus_tag="ACZPDN_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298189.1"
                     /translation="MSDSQITIFYSWQSDLPGSETRNIIQDSIKDAVRLLRDTVDIEA
                     DRDTKGEFGSPDIAQTIFSKIDDCNIFVADVSAVCRYEATDKDGNKKVKYMPNPNVML
                     ELGYATHVVGWDNVICVLNADYGAPKDMPFDIASRRLTPFSLKDGKSKGEIKRYIKGV
                     IQDTVENILENGKRVKIGFSDLRLGCYDDGSISNMLHPIEVSNSVSFVKHKTQILDES
                     LKLVEQIRSMKITQPSELLNTEAASNADEKQTSNATAIIRKDGSVLTPVTSSLKLNLF
                     KLQRVHIKDEDRNTIIELCKNYLGIDIYEDTDFFNIGNLERKYDLMSSYSYEGTKDEE
                     NKYNSIMMLENNLHRVRILDWYATTFDGLFFIPLAIENASKVYDEDVDVYVKINKGVV
                     EVIIPSKELINPDMRGLEGLIYEEDVIKELLMMPESSDISYDTDISYTLADSLAESQA
                     LVRAQFSGAGINGNPRYNSDDYGREIIKYIAMPKAKSNDIEFEFSIGSLRAKEKKWLG
                     PALLINPLTDNFHIEYSIKSKNSDGDLSGIVTYTK"
     gene            complement(6274..7095)
                     /locus_tag="ACZPDN_05905"
     CDS             complement(6274..7095)
                     /locus_tag="ACZPDN_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298190.1"
                     /translation="MKEIEGTTEFLRRKSKLYKNNHGKTQMQIVVKGSHQGFRRYPMG
                     TGNHSCLSRGDYESMSHQGNKEAIASLDKIKLNIGNDVVEVYVSDIELEKEVKCNNRE
                     YEIDIYIKIDRTEPEEYKNFWNGELWLEVFHTCKVDRKQAEDFAIERLPLFETKIPDT
                     YTFYENITLEGYKKRKKQIIEKYKQFGVNGIFFSFNKKFFSVKWRLSENGNYTAYIGD
                     RNFTIIKSKYDDGYGIVYGEKKPLWEYNGKRFNSIEDAKKNAEYVAFLLYNNEKM"
     gene            complement(7274..7825)
                     /locus_tag="ACZPDN_05910"
     CDS             complement(7274..7825)
                     /locus_tag="ACZPDN_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298191.1"
                     /translation="MRGTRLLTKDIFIDTDCISAFLWVGNESLLSQLYPGRVVMPKPV
                     YDEIDRPALAWMKARVDSMINAGKLTVVELTSGTDEFDLYYKLTVNPGEGHKIIGDGE
                     ASSIALAKVKNGIVASNNFNDIFTYINEYSLEYTTTADILVDAYKKGIIDENQGNTIW
                     ANMLRKRRKLGAVNFSDYLSTHS"
     gene            complement(7800..8540)
                     /locus_tag="ACZPDN_05915"
     CDS             complement(7800..8540)
                     /locus_tag="ACZPDN_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR1298192.1"
                     /translation="MRDINEIDSMAVNLRKELGEDSMSPIDIFAIAKTMPELTTVLYP
                     LGSNISGMCIKGEGASLIAINSGMSLGRQRFSMAHEFYHLHFDSEEKKSVCSIAINGG
                     DEKERKADIFASHFLLPSAALYNVLKDSNAVSLEKVVWLEQHFGMSRQAILYRLKSEG
                     KIDSNLYNKMQVDVQYSAAKLGYDTDLYKSTPAGNNMKTTGQYIRMADKLYSEEIISI
                     GKYEEILLDAFREDLVFGDDNEGDEIID"
     gene            complement(8537..8878)
                     /locus_tag="ACZPDN_05920"
     CDS             complement(8537..8878)
                     /locus_tag="ACZPDN_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1298193.1"
                     /translation="MDRLSIGNKIKELRTKAGFNQSIIAEYLQVDQSLISKIEKGERE
                     ASTDVLKKLAELFGCKVSAIINEEEDISCLNVAFRTNGMSKDDLNSIAVINRIALNLE
                     NMKRMLEGLSK"
     gene            complement(9185..9340)
                     /locus_tag="ACZPDN_05925"
     CDS             complement(9185..9340)
                     /locus_tag="ACZPDN_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298194.1"
                     /translation="MASGALDGFVGDDPTISGVSSVWKVIKNGVPEMFKQGPDSRFFN
                     GKENEHI"
     gene            complement(9368..9523)
                     /locus_tag="ACZPDN_05930"
     CDS             complement(9368..9523)
                     /locus_tag="ACZPDN_05930"
                     /inference="COORDINATES: protein motif:HMM:NF036995.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HIR1298195.1"
                     /translation="MRQFYLIFPNGYALRSELNWTHYRLLMRVENDKAREFYMQEAVK
                     SKWSTRQ"
     gene            complement(9477..9677)
                     /locus_tag="ACZPDN_05935"
     CDS             complement(9477..9677)
                     /locus_tag="ACZPDN_05935"
                     /inference="COORDINATES: protein motif:HMM:NF036995.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HIR1298196.1"
                     /translation="MVEAYWNIGKSIIEEQGGNEKAEYGTGLLKELSKQMIQDFGKGF
                     TVANLKKYEAVLFDISKWLRTA"
     gene            complement(10203..10406)
                     /locus_tag="ACZPDN_05940"
     CDS             complement(10203..10406)
                     /locus_tag="ACZPDN_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298197.1"
                     /translation="MIKEYIEAKYGFKVHTAYIEKVKRDLGLPMYDAPSAIEELKQPR
                     KHPTAEKVEAIKDALKHFEVIKL"
     gene            complement(10434..>10620)
                     /locus_tag="ACZPDN_05945"
     CDS             complement(10434..>10620)
                     /locus_tag="ACZPDN_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR1298198.1"
                     /translation="RVFDIIQNNLHYYDSFTKALHVKTVVLLSHKKPDSHINVKVEFG
                     EDKGKVPLDNIAKRVEE"
BASE COUNT         3366 a         2337 c         1371 g         3546 t
ORIGIN      
        1 aataaaccac ctaaagtaca tacaatactt gctagaacaa gccaaataac aggtacaatg
       61 atataaacta catttagtat aatcacacca ataaagcaac taaaacaagc aatcattaaa
      121 cccatatgaa tcttatgata ttttctaaat aaatcttcat accttcttat acaaggatcc
      181 acttgagaat ttatataatc atccgcagac atattattta acatcctcac tcgatttacc
      241 agcaagaacg gcattaattt tatccttagc cattttaacc gtactttgat ctggaaccat
      301 gacagatgcc gcatcaccaa gttcagcaca cattaaagta tcccccgtac catcaaccat
      361 atactgctcc atcttccaag ttggattgtt gtttaattga tacttaatta aatctgtaat
      421 ctcctcattg gacattgtcg tagagaaagt atttgccata gtatccataa tggctgcata
      481 gtttgtgatc acacttggtt tagtagcctc ttttacaata cccataagga cttgttgttg
      541 attcttagta cgctgacgat ccccttctgt atatgaataa cgttcacgtg caaaggctaa
      601 tgcttgttct ccatttaagt gattgacacc ttcgggtaca ctaaatcctg ataaagcagc
      661 aaaactattc gtcgcataac caggttctac atatacgtca actccaccta atacatctac
      721 aagttctgtt actgtatcaa agcctacttt aaatgtatag ttgatctcaa tacctaacaa
      781 ttgttctaca gtacgctttg tcgtatttgt accatgaata cctgtatgtg taatcttatc
      841 taatgctcct tgcatacatc catcgcctgc atcacaagct gtagtgacat agaaatcacg
      901 tggtacagaa gttaatagaa tggtgtgtgt tttaggattg actgtagcta tcataatcac
      961 atcactacga ccattttcat caaatccacc acgcgtatcg gatcctgaga tcaatacatt
     1021 aaatggtttt gaagtaatat cactaactga cgttgcagaa tcattatttt ttactttata
     1081 agaagtttga tagacaacac gcgtatttga atcaaagtta gaatatgctt ccatatccaa
     1141 aatttgacta cgactcgatt cgttaataat aatcgaatct acttctccat tatagaaagc
     1201 ttctaaaagt gttgtcatac tatcgtattc tgtttgattt aaaacaatac cttcactact
     1261 caattcttta agcgcttttt tagaaccctg ttcattcaaa cgtaaactac ccacacttac
     1321 accattcaat tgagacttat tcttaatgga acttgattct ttgacaacca cactcactgt
     1381 atttttggca tgtttggcaa catttgtaat actacttaaa acattttgcg ttttagaaat
     1441 ataataccca cccatacaag atgcgacaca aataaatagt gttaatataa tacaaacaat
     1501 tttagaaacc acattcttac taaaataaat aagcaataag ataaaaatgg catccaagac
     1561 aaccactcca atcgtaagtg gaaataagaa tttagatggc aatacattca aacgaatcac
     1621 ttgatacaat aagtaaaatg aagaaatcac caatacaaca ctcaataaaa tactcaatac
     1681 tttcgaaaat ttacttttca taattaaaca gctccccttc catataaaac ttgttttact
     1741 gtcttacaaa gcaatgtaac atccagccaa aatgaccagt tacgataata atactcatcc
     1801 aaaatcagac gttgttcaaa actcacatca ctacgaccat gactttgcca catacccgta
     1861 atgccaggct tacacgcaac cacatcctca taatattcac ccatatcgac tttttctttt
     1921 ggtagatacg gcctaggccc aattaaagac atctggccaa agaacacatt gataaattga
     1981 ggaaattcat ccaaactctt ctcacgcaag aaatgccctg cacttgtaat acgaggatca
     2041 ttggaaagct ttttattctt ctgatactct tcacgaatct taggatcact tgccattaaa
     2101 tcttctagaa tcttatctgc accaagcacc attgtacgat acttatacat cttaaattcc
     2161 ttgccatttt taccaatacg attctgcgta aagaagacag gcccccgatc gccttccata
     2221 cgatttttaa agaatacata taccgttaaa ggaatcaaga tcatcatgcc acatagccct
     2281 gcacaaatat ccatacaacg ctttaaaata taagatccca attgattgac accacgagaa
     2341 tttgaaatca ttaatatacc atcaaaatcc tcaaccttcg tatcaaacgt aactaagcca
     2401 gtgacctgcg gcaaatattt aatgttcaat accttacctt taaataactc aaacaaacga
     2461 tttaaattct ttctgttaat ttctggaatt gcaatcaata ccgtatcaat attatgcgta
     2521 cgtataaaat catctaccgc atctaaatcg atcgtctgct ttttagaaac aacctgctct
     2581 tgatgaattc catacgactt acaactcaca tatccctgta catccaataa tgaataacta
     2641 tttccacgac atacaaaagc caattgcgca gccgtatgac ccgtaccaac aattaacgta
     2701 ttatgtttat aacgctggcg gaatatctta tgcgaatatc ttgagttaca acccgcaaac
     2761 aaaaataaga caagtgttaa taataaaaac ccatatattt tacgaatcgt ccacgaacta
     2821 aatgggaaaa tcaaaaaact accaaagaaa aaacccaaat gcgaaagaat caataaacga
     2881 atctcatcat acatcaataa cacttttcct tgataacgcc caatcaacaa ctgcattaaa
     2941 aaatacaaag cacaaagctt aatcgtatat tccccataaa aacgcaagac attgatctcc
     3001 tgcaggaaat taattaatat ccaataaaat ataaattcta tcgttatctt taaagtacct
     3061 atttttgaca tagtccaaat ctccaaatat ataactttta ttattatata aaaaaaagaa
     3121 gaaaagcaca aatttacaac gtgccttctt ccattctcta ctcatgactt gatccatttt
     3181 ttgtttttat aatcatattt ataaccaaaa acaatgagga aagcatttac catttgaaaa
     3241 taagccatcc tcttattaag acaacgaatt tcttcaagtt agctagatac atgctgattg
     3301 atgtcgcaat atcagatcaa atctctccat tttgagccaa ttttcgatta ttttcggata
     3361 catatcaagt tttccctgtt gctatgcaaa tggtaaaaag cgcagttaca tctgcaacca
     3421 atgctttttg cagattatta tttgcggtga aatgtccaag cgaattcatt ggaacaatcg
     3481 cggctgcaaa tctgttgtct ggcgttgctt cagcagacta gaatcaaccg gactggaaag
     3541 ccatgcccga accatcaatg agccggtcct tcaggatacc gtcgtcaaag caatcaacca
     3601 gatgcttgga gacaaaagta gctatcaggc acagcagcag cttaatatag cctcggttat
     3661 ccgagcttca caggccactt ccgttgaaaa catcgatgag aagctgatgg ccttgcagca
     3721 agagcttatc cagaaagctc agagcaaaga agtctacgac gagattgctg atgacatttt
     3781 caggcttcga gaactcagac aacaaaccac cattgaaaat gctgcaaggg ataagcagat
     3841 taagcgaatc aatgaccttc aggattatat tgcacaacag actacccgcc ttactgcatt
     3901 cgatgaatca ctggttcgac gctggctcaa acagattacc gtctggatga ccacttaacc
     3961 gtagaactaa aatccggcat cagcattgat attgatgcat aagccctcta gacgcacgaa
     4021 ggctccccac cactagataa tctccggtga tggggagcct ctctttattt agtgtatgtt
     4081 acaattccag acaaatctcc atctgaattc ttggatttta tagagtattc gatatggaaa
     4141 ttgtctgtta acggatttat taacaaggct ggtcctaacc atttcttttc ttttgctctt
     4201 aaagatccaa tagaaaattc aaactcaata tcgttgcttt tagctttagg cattgcaata
     4261 tactttatta tctccctgcc ataatcatct gaattatatc gaggatttcc gttgattcca
     4321 gcaccgctga actgagctct cactaacgcc tgactttcag ccaaactatc tgctaaagtg
     4381 tatgatatat cggtatcata agaaatatct gatgattcag gcatcatcaa aagttccttt
     4441 ataacatctt cttcataaat caaaccctct aagcctcgca tatctggatt tatgagctcc
     4501 ttcgatggaa taatcacttc tacaacacct ttatttatct taacgtatac atctacatcc
     4561 tcatcgtaca ctttagatgc attttcaatt gcaagtggta taaaaaacaa tccgtcaaaa
     4621 gtcgtcgcat accaatccag aattcgcaca cgatgtaaat tattttcaag catcatgata
     4681 ctattatatt tgttctcctc gtctttcgtc ccctcataag aatatgatga cattaaatca
     4741 tattttcttt ctaagtttcc aatattgaaa aaatctgtat cttcatatat atctattcca
     4801 agataatttt tgcacaattc tattatcgta tttctatcct catccttgat atgtaccctc
     4861 tgtaatttga aaagatttaa tttcaaagag ctagtgacag gagttagaac tgagccatct
     4921 tttctaataa tagcagttgc attgcttgtc tgtttctcat ctgcattgct tgccgcttct
     4981 gtatttagga gttcagatgg ttgtgtaatt ttcatacttc tgatttgttc taccagtttc
     5041 aaagattcgt ctaatatttg tgttttgtgc ttaacaaaag agactgaatt tgatacctca
     5101 atcgggtgaa gcatattaga aatagagccg tcatcataac aacccagacg taaatctgaa
     5161 aaacctattt taacacgctt tccattttct aatatgtttt cgacagtatc ttgtattacc
     5221 ccttttatat atcgttttat ctctccctta cttttcccat cttttaatga aaatggggtc
     5281 aatcttcgac ttgcaatgtc aaaaggcata tcctttggtg cgccataatc agcatttaaa
     5341 acacatatta cattgtccca tcctacaaca tgagtagcat atcctaattc aagcattaca
     5401 ttaggattag gcatatattt tactttcttg ttcccatctt tatcagttgc ctcatatcga
     5461 catacagcac taacatcagc aacaaaaata ttgcaatcgt cgattttcga gaaaatagtc
     5521 tgtgcaatat caggtgaacc aaattctcct tttgtatcac ggtcagcttc aatatcgact
     5581 gtatctctca gtaaacgaac tgcatctttt atactatcct gaattatgtt tctagtctca
     5641 cttcccggca aatcagattg ccaagaataa aaaattgtaa tctgtgaatc actcacatta
     5701 ccgcctccta tctccgttat ctattccact gccaagtcgg ccccttagta tcgaatggtc
     5761 tgaactacgg tcggctctga caacaatcct agtgccacaa cctctgacat ctaatccaca
     5821 gcctaaaccc taccgctatt ctccggtact caatttctat ccagtcagac cgaaaagtag
     5881 atatgtttcc atataataat accgagcttt ccgcaaggct acttacgatg atgccaacct
     5941 tacaaaaaga ccggaaatat gccactttta aacctttatt tatctttcct tgatagcaaa
     6001 cataccatct caacatgcag aatgttccga cagggcttta acattatgcg aactacgcgt
     6061 aaaatcgggg ttttaccttt tttagagatt ttcgcactaa ccgcagtcaa ccagttacta
     6121 cttggcgtac agactgccta actgaactaa taaacagcag gtacgccgat ttattgttac
     6181 acctttactg actaataacg ttaaaaattc tgctgccggt aaaagcatta ttcttgtccg
     6241 tatatttctt taggccataa attgaaatag attttacatc ttttcgttat tgtacagcaa
     6301 aaaggccaca tactctgcat tcttttttgc atcttcaatg gaattgaatc tctttccatt
     6361 atactcccat aatggcttct tttctccata cacaatacca tagccgtcat catatttgct
     6421 tttaataatt gtaaagttac gatctcctat atacgcggta taatttccgt tttcgcttaa
     6481 tctccatttc actgaaaaga attttttatt gaatgaaaag aatattccat ttactccaaa
     6541 ttgcttatac ttctctatta tttgtttttt ccttttttta taaccttcca atgtaatatt
     6601 ctcatagaaa gtatatgtat ctggtatttt tgtttcaaat agaggcagcc tttctattgc
     6661 gaaatcttca gcttgttttc tatctacttt acatgtatga aatacctcaa gccataattc
     6721 gccattccaa aaatttttgt attcttcagg ctccgttcta tctattttaa tataaatatc
     6781 aatctcatac tctcgattat tacattttac ttctttttct aattcaatat cactaacata
     6841 aacctcaaca acatcgtttc caatatttaa ttttatttta tccaatgaag caattgcctc
     6901 tttatttccc tgatgtgaca tactttcata atcacctcgc gacaaacacg aatgatttcc
     6961 agttcccata ggataacgtc taaatccttg atgtgaacct ttcactacaa tttgcatttg
     7021 agttttccca tgattgtttt tatataactt ggactttctc ctaagaaatt ctgtagttcc
     7081 ttctatttct ttcattgccc aataacttgt ttcaaaataa ccttgcttcg cttcatcata
     7141 ataatagcaa aacattctca tgtctgatta cctccacaat agcaattatt tggtaaggac
     7201 tacttttcct gcactactaa ttgcaaaaaa cgcctacaaa tctgtaggcg ccaatataat
     7261 tcttgatatg taattatgaa tgtgtactca agtagtctga aaaattaaca gcacctaact
     7321 tacgtctttt tcgcaacata tttgcccata tcgtatttcc ttggttctca tcaataatcc
     7381 cttttttata cgcatcaaca agaatatctg ctgtagtagt atactccaag gagtactcat
     7441 ttatataagt aaaaatatca ttgaagttat tgctagctac tattccgttt ttaaccttag
     7501 ctaatgcaat tgacgatgct tctccatcac caataatctt atgcccctca cctggattta
     7561 cagtcaattt atagtacaaa tcaaattcat ctgttcctga cgtaagttca acaacagtca
     7621 atttacctgc attaatcata ctatcaacac gagccttcat ccaagctaat gccggtctat
     7681 caatctcatc atatacaggc tttggcatca ctactcttcc tggatataac tgcgaaagaa
     7741 gactttcatt accaacccat aagaaagcag aaatgcaatc agtatcaata aaaatatcct
     7801 tagtcaataa tctcgtcccc ctcattatca tctccaaaga caagatcctc tctaaatgca
     7861 tccaaaagta tttcttcgta ctttccaata gatattattt cttcactata aagcttatct
     7921 gccattctga tgtactgtcc ggtagttttc atattattac ctgcaggagt cgacttatat
     7981 aaatcagtgt catagccaag ttttgcagcc gaatactgaa catccacctg catcttatta
     8041 tataagttac tatcaatctt accttcgctt tttaatctat ataatattgc ctgacgactc
     8101 ataccaaaat gctgttctaa ccatactact ttctctaaag aaactgcatt actatctttc
     8161 aatacattat acaaggcagc cgatggaagt aaaaaatgag aagcaaatat atcagcttta
     8221 cgttcctttt catctcctcc attaatagct attgaacaaa cacttttttt ttcttctgaa
     8281 tcaaagtgta aatgataaaa ctcatgagcc atcgaaaacc tttgtcttcc aagtgacatt
     8341 ccagaattaa ttgctattaa actagcacct tctcctttta tacacatacc actaatattg
     8401 ctgcctaaag ggtacaatac agttgtcaat tctggcatag tcttcgcaat cgcgaaaata
     8461 tcaataggtg acatactatc ctcacctaat tcttttcgca aatttactgc catactatca
     8521 atttcattaa tgtctctcac ttgcttaacc cctccaacat tcgtttcata ttttcaagat
     8581 tcaaagcaat cctatttatg acagcaattg aattcaaatc atccttggac attccatttg
     8641 ttcggaacgc tacatttaaa caagaaatat cttcttcttc attaataata gcactcactt
     8701 tacagccaaa cagctccgca agtttcttta aaacgtcagt acttgcctct ctttcacctt
     8761 tttcaatctt tgaaattaaa ctctgatcaa cctgcaaata ttcagcaatt atagattgat
     8821 taaagccagc ctttgttctt aattccttaa ttttattacc aatgcttaat ctatccatag
     8881 tatcgcgcct cctctttatt atattatatc gcctttttgt cataatgtaa atattttatc
     8941 tatccatttt tatgacaagt atgtatatac tatctccaaa aaggggcact gtcaatcaat
     9001 ttttgaagat tttctaagca ataataataa aaactagcag tattcttcaa ctcaactatc
     9061 tcttcggctt aaatttagca ctatacgcac ttactgcctc atccttcatt aaccatccga
     9121 gcttcctaaa gaactccagt ttctgttcat cagtaaccca ttcttgtaaa gtatgaagca
     9181 ctccttaaat gtgttcattc tctttcccgt tgaaaaatct actgtctggt ccctgtttga
     9241 acatttctgg aactccattc ttaattactt tccatacaga agaaacgccg gatattgtag
     9301 gatcatctcc aacaaatcca tcaagagctc ctgaagccat tttttaaata agttttttct
     9361 tcgtttgtca ttgtctagta ctccattttg attttacagc ttcctgcata taaaattctc
     9421 gtgctttatc attttccaca cgcatcaaaa gccgataatg agtccaattc aattcgctac
     9481 gcagtgcgta gccatttgga aatatcaaat aaaactgcct catatttttt caaattagca
     9541 acagtaaatc cctttccaaa atcttgaatc atttgttttg ataattcttt caataaacct
     9601 gtcccatact ccgccttttc gttaccgccc tgttcttcaa tgattgattt tccgatattc
     9661 caataggctt caaccattgg aaattggctg tttgatagac tttatttctt gctgttaaca
     9721 agatgttctt tatctcatca taaaattttt gttccataaa aacctccgca agacacctag
     9781 ttttatttgt ataaatcctt tctcaatcga tttacaagta tattaaattg ttccacactt
     9841 ttctggtaaa taaatttttt taattccttg acaagaccat catcttttga aattaacttc
     9901 aacttaggaa ttgccatctt caaatcattt agtgcattat ttctattttc atcatctgat
     9961 agctcttgta tattatacct tgctagtcct aaagaatatt tcgcttcaac tggaatagta
    10021 caatttgcgc aaatacaatt atatattcat atactctctt tttttcctca aaaacatact
    10081 ttttcttatc ttgcaatggt tgaaaaataa aagtggacaa tatcaacgtt attacaaaag
    10141 aacctaatat actaataatt atggaaatta cattatcaat actcatttgc actccttccc
    10201 tgctacaatt taatcacttc aaaatgcttc aatgcatcct ttatcgcttc cactttctcc
    10261 gctgtcggat gcttccttgg ctgtttcaat tcttctatcg cactaggagc atcatacatt
    10321 ggcaagccta aatctctctt taccttttcg atatatgcag tatgtacttt aaagccgtat
    10381 ttagcttcta tgtactcctt tatcattctg taggtcgctt gttctttgag cttttattct
    10441 tcgactcttt tagcgatatt atcaagcgga acttttccct tatcttcgcc aaactcaact
    10501 tttacgttga tatgactatc tggttttttg tgggaaagca gtactaccgt cttgacatgt
    10561 aaagctttcg taaatgaatc ataataatgt aaattatttt gaatgatatc aaatacccta
//
LOCUS       DBMDWL010000067        10599 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2176_length_10599_cov_2.578872, whole genome shotgun sequence.
ACCESSION   DBMDWL010000067 DBMDWL010000000
VERSION     DBMDWL010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10599)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2176_length_10599_cov_2.578872"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="ACZPDN_05950"
     CDS             complement(<1..791)
                     /locus_tag="ACZPDN_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013695.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR1298199.1"
                     /translation="MKEKQEQIVMRISFWSGVAFALAELIMAIYSKSQSVLADTVYDS
                     TELVVIGLTIYIIPLFYKPVTEKHPFGYAQLESIVILLKGFMFIAVMMVLIMNNIQIM
                     LYGGNEIDHMQVSMFETILTCFSALILFILMQINKKVTSPTVDVEIYGWKIDVFSSVG
                     VSLAFFLSSFLTHTPLAFISPYFDQIVAIVLALGMMKEPIQMIGDSFRSLMLLSPDEE
                     EVTKIKHISSSILNNYPYDPVFYDITTTGRKMWISIYFTTRVDTMS"
     gene            complement(796..2091)
                     /gene="iscB"
                     /locus_tag="ACZPDN_05955"
     CDS             complement(796..2091)
                     /gene="iscB"
                     /locus_tag="ACZPDN_05955"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HIR1298200.1"
                     /translation="MVYVLDIDGQPLMPTSRHGKVRRLLNSHLAKVVKRCPFTIQLLY
                     QSTKETQPVSLGVDAGSRHIGLSATTEREVVYQEELVPRNDIVKLLSARRALRRSRRN
                     RKTRYRKVRFNNCVHSKHKGWLAPSVEVKIQEHITAIKRICQILPISDIHVETAEFDL
                     QRLKAMEEGRLLPAGTDYQLGEQYNFYNTRQYVLHRDEYTCQCCGTHDNNVKLHVHHV
                     ESRQTGGEAPNNLITLCEHCHKSIHAGKMMLPKGKKRGKSYRDAAFMGIMRNTLLERL
                     RKEVNVPVTMTYGYITKYWREKAGLEKSHINDAICISKHPYARPLETYYLTKAVRHHN
                     RQIHKTKFSKGGIRKRNQAPYLVKGFRLFDKVLYQGESYFIFGRRITGYFDIRTLDGT
                     KVNKGSTSYKKLRIQDTAEAYLTEVRAIPHMNEFTCVLA"
     gene            complement(2426..4342)
                     /locus_tag="ACZPDN_05960"
     CDS             complement(2426..4342)
                     /locus_tag="ACZPDN_05960"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR1298201.1"
                     /translation="MQNLTYKILEKHLLKGKLEAGQPIEIKMDQTLTQDATGTMAYMQ
                     FEAMGVKKVKTELSVSYVDHNTLQNGFMNADDHAYLQSVASKYGIYFSKAGNGICHQV
                     HLERFAKPQKTLIGSDSHTPTSSAIGCIAIGAGGLDVAKAMAGIGYRLTCPKVVEVKL
                     TGTLAHGVSSKDIILKLLELLSVKGGVGKVFEYTGEGVKNLSVYQRATITNMGAELGA
                     TTSIFPSDEVTHEFLKRQQREEDYIPLSADEGCHYDETVEIDLSKLVPLAACPNSPDA
                     VKNVSELSGMKVDQVAIGSCTNSGYEDLMKAAAILKGKKIAHNVSLVVSPGSRQVLMK
                     LIENGTLSTFVSCGARILECTCGPCIGMGQSPKSDAVSLRTFNRNFYGRSGTLSAGIY
                     LVSPEVAAASAITGVITDPTTLDYADEFEKEQSYIDDSLIYAPSKNPENVEIVRGPNI
                     KPLPVNTPMDQTIDKKVVIKLPDNITTDHIAPAGAKVLPYRSNIEKLSTFIFENNKPH
                     FDEVCKENNGGIIVAGENYGQGSSREHAALAPMYLGIKAVLAKSFARIHLANLVNFGL
                     LPLVFDDKSDYDKIDEMDELKIENVDSLYNSFDLTIENVTKKETYKVHAPISKEDFEI
                     LMAGGALNYIRNQQ"
     gene            4437..5711
                     /locus_tag="ACZPDN_05965"
     CDS             4437..5711
                     /locus_tag="ACZPDN_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR1298202.1"
                     /translation="MHTLKEIYQDNEERNFIDPSLYSRYRVKAGLRNDNGTGVKVGLT
                     KICDVIGYKIIHEKKYNIDGQLIYRGYPINYLVNKQPSDKICGFEETAYLLIFGTLPN
                     ADQLKTFKDYLLNFKMGPVDLQYETPNILNALQIEVLKLYAKDPSADTDDLEERMMKG
                     LKILASIPLFTFSYARNKVFKSYPYPDRSLAENILCMARGNDQYTFEEAKIMDTLLMV
                     HADHGGGNNSTFANVVISSTGTDIYSCISAAIGSLKGPKHGGANCKVTNMFKAIFHEV
                     GFTTDKATLKKIANRILDKDFFDHSGLIYGIGHAIYTKSDPRALLIHEQCEALAKSKG
                     QEDIYNCIHAFEKVAIEVMKERKGIDVCANVDYYSGYVYSLLGIEADLFTPLFAISRT
                     AGWVSHHLENRQSNRKLIRPANVYVEEMEEIK"
     gene            5708..6703
                     /locus_tag="ACZPDN_05970"
     CDS             5708..6703
                     /locus_tag="ACZPDN_05970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR1298203.1"
                     /translation="MTTITVFKGDGIGPEITDAVIKVLNAANAKLEYEVFNVGAKVYE
                     ETGEYIPEKAFESYEKNKILLKSPITTPVGKGFRSLNVTLRNKYDLWANIRPAKSNPC
                     IPTRFDNVDIVTFRENTEDLYVGKEEQIDGNTVYAYKIITKKASTRIIRAAFEYCVKN
                     NRKKLTCVHKANILKMSDGLFLHIFEDIAKEYPQIESNSLIVDNTCMQLVMHPEQFDV
                     MVMPNLYGDIVSDLTSGLIGGLGLLPSCNKGDDYAMYEAVHGSAPDIAGKHIANPSAL
                     LLSACMMLEDLHQESVATKIRNALNKTFEERKVLTFDLGGSASTDAFTDEIIKNL"
     gene            complement(6721..6978)
                     /locus_tag="ACZPDN_05975"
     CDS             complement(6721..6978)
                     /locus_tag="ACZPDN_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298204.1"
                     /translation="MAASKIPVWIKQCLDQYGNCACGRAITKKIGKENLLQILENEGY
                     PCELEILSDQKDKSAYPKDGTYILTLKNRQSLMVDEDEDEE"
     gene            complement(7055..8458)
                     /locus_tag="ACZPDN_05980"
     CDS             complement(7055..8458)
                     /locus_tag="ACZPDN_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="neurofilament protein"
                     /protein_id="HIR1298205.1"
                     /translation="MPIKTTKKVTNKKVVKEETKPAVEAKKEEVKTAEVKKATKPAVE
                     IKKEEVKPAETKKAPAKKTTTKKAAKPAVEAKKEEVKSAETKKAPAKKATTKKAAKPA
                     VEAKKEEVKPAETKKAPAKKATTKKAAKPAVEVKKEEVKPAETKKAPAKKATKSAVKA
                     KKEEVKPAETKKATAKKTTKKATTKKTTTKKVTTKKMTKEEQYAKLSLDTCLDLAKAM
                     SMNVTRDSIIQQLILNPDVKSVSKDLVNKYQLTGKFNFDEDGYDEGLVEVLVSKVYET
                     ADIKPQKAEDLQADVDHALNYKFTDVVADGEEYKAQFDTMRKVLMIAQHKDIHDSKNL
                     DEEIGIDVEKFVEEFMDLAYSVLKTWKYEDVDYYEHFIYAVLSQLEDLHDTYRNRIMM
                     DVADLYILHGDYGLGDADYGYILRENQIKDYIYYRYASIYEGVDKDKAKQIANQALQF
                     VDDRFTYYPNIIAVLEG"
     gene            8614..9738
                     /locus_tag="ACZPDN_05985"
     CDS             8614..9738
                     /locus_tag="ACZPDN_05985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1298206.1"
                     /translation="MQTPPKFKPKTYVPVRNKILTTAGIILLSGIVGFGAGYAGSHLS
                     NNTNVTIQQQTNKSNSGTIQVADVSDIVEKCKDSVVEITTESVSSGNSIFGQYVSQGA
                     GSGVIISEDGYIVTNNHVVSGATSLKVTTTDGTEYDASIIGTDSQTDLAVIKIEAQNL
                     LAATLGDSDILQVGDPAIAIGNPLGELGGTVTTGIISATDRQITIDNETMTLLQTDAA
                     INPGNSGGGLFNADGNLIGIVNAKESSTGIEGLGFAIPITPAKDVITELMQNGSVTSR
                     PALNVSLYDYTSSNQTQNSKYEDGCYIVQIVKNGAADKAGLKQNDRIIRFDGEKIQST
                     SDVKAILKKHKIGDNVKMVVDRDSKEIEVEIVLQAQTQAN"
     gene            9813..>10599
                     /locus_tag="ACZPDN_05990"
     CDS             9813..>10599
                     /locus_tag="ACZPDN_05990"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR1298207.1"
                     /translation="MSEKIKKRKIMLVGTGFVGMSFAYSMLSEKGIDELVLVDVNEDK
                     ARGEQMDLSDGLVYADTKMKITAGTYADAADTNIVVLTAGAAQKPGQTRLDLVKINAN
                     ITKGVCQDLKENNFNGILVVANNPVDIMTYVAWKESGLPKNHVIGSGTVLDTARLRHA
                     LSEKLGFADSNIHAYIMGEHGDSSFVPWIHSYIGCKNLLEYLDENNISLSELQEIYID
                     VRDKAYKIIELKRATYYGIGLALKRIVSCILNNERAILPVSAYQ"
BASE COUNT         3323 a         1894 c         1805 g         3577 t
ORIGIN      
        1 gacattgtgt caactcgtgt agtaaagtaa atagatatcc acatttttct tcctgttgtc
       61 gtgatatcat agaagacagg gtcataggga taattattta atatagatga agaaatatgt
      121 tttatttttg tgacttcttc ttcatctgga cttaaaagca ttaaacttct aaaactatct
      181 ccaatcattt gaataggttc tttcatcatg cctaatgcca atacgattgc cacaatttga
      241 tcaaaatagg gtgatataaa agcaagtggc gtatgtgtta aaaacgaaga taagaaaaac
      301 gctagcgata caccaacact tgagaataca tctattttcc atccataaat ttcgacatcc
      361 actgtaggag atgtcacctt tttattaatt tgcataagta tgaataaaat taatgctgaa
      421 aagcatgtta aaatcgtttc aaacatagaa acctgcatat gatcaatttc atttccacca
      481 tacaacataa tttgaatatt attcataatc aaaaccatca taaccgctat gaacataaat
      541 ccttttaata aaatcactat actttcaagt tgtgcatatc caaagggatg cttttcggtt
      601 actggtttat aaaataaagg aataatataa attgttaatc caataacaac aagttcagtt
      661 gaatcataaa cggtatctgc tagaaccgac tgggatttag aatagatggc cataatcaat
      721 tctgcaagtg caaatgcaac accggaccaa aaagaaatac gcattacaat ttgttcctgt
      781 ttttctttca ttattctaag caaggacaca cgtgaattca ttcatgtgag gaattgctct
      841 cacctccgtc aaatatgctt ctgctgtgtc ctgaatcctc aatttcttat agctggtaga
      901 acccttattt accttggtac catcaagcgt tcttatatca aaatatcctg ttattcgcct
      961 tccaaagatg aaatagcttt cgccttgata caagacctta tcaaacagac ggaatccttt
     1021 tacaagatat ggcgcctgat tgcgtttacg aataccacct ttactgaatt tggtcttatg
     1081 aatctgtcgg ttgtggtgtc gaaccgcctt tgtcaggtaa taagtctcca aaggtctggc
     1141 atatggatgc ttactgatgc agatggcatc gttgatatga cttttctcta agccagcctt
     1201 ctcgcgccag tattttgtga tgtatccata tgtcatcgtc actggaacat ttacttcttt
     1261 tcttaaacgt tctagcagag tattacgcat gatacccata aaagcagcat cacgatagga
     1321 cttgcctcgt ttcttgcctt ttggcagcat catcttcccg gcatgtatcg atttatgaca
     1381 gtgctcacaa agtgtaatca gattgttagg tgcctctcca cctgtctgac ggctttcgac
     1441 atgatgcaca tgtagtttta cattgttatc atgcgtacca caacactgac aagtatattc
     1501 atctcgatga agcacatact gtcttgtgtt atagaaattg tactgttccc caagctgata
     1561 atctgtacca gcaggaagta gccttccttc ttccatcgct tttagacgct gaagatcgaa
     1621 ttctgctgtt tctacatgaa tatcagatat aggcagaatt tgacagattc tcttgatggc
     1681 tgtgatgtgc tcctgtatct tgacttctac agatggagcc agccatcctt tgtgtttaga
     1741 atgcacacaa ttgttgaatc taacctttct gtatcttgtc tttcgattcc ttcgagaacg
     1801 gcgcaatgct cgtcttgctg ataacagttt tacaatatca ttgcgaggaa caagttcttc
     1861 ctgatagaca acttcccgct ctgttgtagc agacagacca atatgtctac ttcctgcgtc
     1921 tacaccaaga gatacaggct gtgtttcttt tgtactttga tataaaagct gtattgtgaa
     1981 tggacaacgc tttactactt tagcaagatg actattcaac agccttctta cttttccgtg
     2041 ccttgaagtt ggcataagtg gctgtccatc gatatctaat acatatacca tataaggcac
     2101 ctcctttttt gtgatatgct taaagcggta agccggcaca tatcgagccg tatatgacac
     2161 cttcgccaat gttaataaga ggtttgcaat aagacacaca cagttcctac cctacagaac
     2221 tttttaatgt atcttcttag agccgcagac taggtgttac atccacgggt aactatatat
     2281 tctctcttaa cgtagtgccc gaagcactta ggctagtcaa cctaggcttt tacaagcctg
     2341 ccaattcatt ggcgggtagt tgactctctc acctaatttc ctagatatta taccacaaga
     2401 aaaggaacgt ctttaaaaaa cgtccctatt gttgattacg gatataattc agtgctccac
     2461 ctgccataag aatttcgaaa tcttctttac taataggtgc atgcacttta tatgtttctt
     2521 tctttgtcac attttcaatc gttaaatcaa atgaattata aagtgaatct acattttcaa
     2581 ttttcaattc atccatttca tcaattttgt cataatccga tttatcatca aatacaagtg
     2641 gcaacaagcc aaaattgact aaattagcta ggtgaatacg ggcaaaagat ttagccaaaa
     2701 cagcttttat acctaaatac attggtgcaa gtgctgcatg ttctctactt gatccttgac
     2761 catagttttc gccggctaca ataatacctc cattattttc tttgcatact tcatcaaaat
     2821 gcggcttatt gttttcaaat atgaatgtag atagtttttc aatatttgag cgataaggta
     2881 aaaccttcgc tccagctggt gcaatatggt ctgtcgtaat attatctggt aatttgatga
     2941 caactttttt atcaattgtt tgatccattg gtgtatttac aggcagtggt ttaatgttgg
     3001 ggcctcgaac aatttcaaca ttttctggat tcttgcttgg ggcataaatc aatgaatcat
     3061 caatatagct ttgttctttt tcaaattcat ctgcataatc taatgtcgtt ggatccgtaa
     3121 taactcctgt aattgcactt gctgcagcta cttctggtga tactaagtaa ataccggcac
     3181 ttagtgttcc acttcgacca taaaaattac ggttaaaggt acgtaaactt acggcatcac
     3241 tttttggact ttgtcccata ccaatacaag gcccacaagt acattctaga attcttgctc
     3301 cacaagatac aaaggttgaa agtgttccat tttcaattaa tttcataagt acttgtcttg
     3361 atcctggtga aacaactaga cttacattat gcgcaatctt tttacctttt aaaatagcgg
     3421 ctgccttcat taaatcttca tagcctgaat ttgtacaaga accaattgct acttgatcta
     3481 ctttcatacc agagagttct gatacatttt taacggcatc tggtgaattt ggacatgcgg
     3541 ctaatggtac taatttgctt aaatcaattt caacagtttc atcatagtga caaccttcgt
     3601 ctgcacttaa aggaatataa tcttcttctc tttgttgacg ttttaagaat tcatgggtaa
     3661 cctcatcact tgggaaaata gatgtagtag ctccaagttc agctcccata tttgtgatgg
     3721 ttgctctttg gtaaacactc aaatttttga caccttctcc agtgtattca aaaactttac
     3781 caacgcctcc ttttacactc aataattcta atagctttaa aataatgtct ttactagaaa
     3841 ccccatgcgc tagagtacct gttaatttta cttcaacaac tttaggacat gttaatcgat
     3901 agccaattcc agccattgcc ttggcaacat ccaatcctcc tgcaccaatc gcaatacatc
     3961 ctattgccga tgatgtcggt gtatgtgagt cggatccaat caatgttttt tgtggtttgg
     4021 caaaacgttc caaatgaact tggtgacaaa ttccatttcc tgcttttgaa aaataaatac
     4081 catatttact agctacactt tgaagatatg cgtgatcgtc cgcgttcata aatccatttt
     4141 gtagtgtatt atgatcgaca tagctaacag ataattctgt ttttactttt ttcactccca
     4201 tagcttcaaa ctgcatatag gccatagtcc ctgtcgcatc ttgtgttaaa gtctgatcca
     4261 ttttgatttc gataggttgg cctgcttcta attttccttt caataaatgt ttttctagaa
     4321 tcttgtacgt caaattttgc atagacaata tcctcctaaa acgaattata acaaaaaatg
     4381 taacataatg aaagtttatg ttataatatg aaacatatga aaggaggttt cataaggtgc
     4441 acacacttaa agaaatctat caagataatg aagaacgcaa ttttattgat ccttccctat
     4501 actctagata tcgcgtaaaa gctgggctta gaaacgacaa tggtacggga gttaaagtag
     4561 gattaactaa aatttgtgat gttattgggt ataaaattat tcatgaaaaa aaatataaca
     4621 ttgatggtca attgatttat aggggctatc caatcaatta tttagtaaac aaacaacctt
     4681 cagacaaaat atgtggattt gaagaaacgg cttatctttt aatttttgga accttgccaa
     4741 atgctgatca attaaaaact tttaaagatt acttactcaa ttttaaaatg gggcctgttg
     4801 acttacagta tgaaacacca aacatattga atgcacttca aatcgaagta ttaaaattat
     4861 atgcgaaaga tccaagtgca gatacagatg atcttgaaga aagaatgatg aaaggattaa
     4921 aaattcttgc gagtattcca cttttcactt tttcatatgc ccgtaataaa gtatttaaat
     4981 catatcctta tccagacaga agcttagctg aaaacattct atgcatggct agagggaatg
     5041 atcaatatac atttgaagag gctaaaatca tggatacttt attaatggtc cacgccgatc
     5101 atggtggtgg aaacaattct acttttgcca atgtcgtaat atccagtaca ggaacggata
     5161 tctactcttg tatttcggct gccattggtt cattaaaagg gcctaaacat ggtggagcaa
     5221 attgtaaggt aactaacatg ttcaaagcca tatttcatga ggttggattt acaaccgata
     5281 aagctacgct taaaaaaatt gcaaatcgta tattagataa agactttttt gatcacagtg
     5341 gacttattta tggtattggt catgcgatct atactaaaag tgatccacgt gcactattaa
     5401 ttcacgagca atgtgaagct ttagctaaat ccaaagggca agaagatatt tacaattgta
     5461 ttcatgcttt tgaaaaggtt gctatcgagg taatgaaaga aagaaaaggc atcgacgtat
     5521 gcgcaaatgt agattattac tcaggctatg tatattcatt actaggtatt gaagccgatc
     5581 tatttacacc attattcgcg attagtcgaa cagctggctg ggtaagtcat catttagaaa
     5641 atcgtcaatc caatcgtaaa ttaattcgac ctgccaatgt atatgtagaa gaaatggagg
     5701 aaattaaatg acaacaataa cagtttttaa aggagatgga atcggaccag aaatcacgga
     5761 tgccgtcatt aaagttttaa atgctgcaaa cgcaaagctt gaatatgaag tgtttaatgt
     5821 aggggcaaaa gtatatgagg aaactgggga atatattcct gaaaaagctt ttgaatctta
     5881 tgaaaagaac aaaattttat taaaatcacc tatcacaacg ccggttggta aaggatttcg
     5941 ttctttaaat gttacattac gaaacaaata tgatttatgg gcaaatattc gtcctgctaa
     6001 atccaatcct tgtattccta caagatttga caatgtagat atcgtaactt ttagagaaaa
     6061 cacagaagat ctatatgttg gtaaagaaga gcagattgat ggaaatacag tttatgccta
     6121 taagatcatc acaaaaaagg caagcacaag aattattaga gctgcttttg aatattgtgt
     6181 taagaataat cgtaaaaaat taacttgtgt gcataaggct aatattttaa aaatgagcga
     6241 tggcttgttt cttcatattt ttgaagatat tgccaaagaa tatcctcaaa tcgaatccaa
     6301 ttccttaatt gtggataaca cttgcatgca gcttgtgatg catcctgaac aatttgatgt
     6361 tatggttatg ccaaatcttt atggagatat tgtttctgat ttaaccagtg gtttaattgg
     6421 cggattaggt ttacttccaa gttgcaataa aggtgatgat tatgccatgt atgaagctgt
     6481 gcatggtagt gctccagata ttgcaggaaa acatattgca aatccgagcg ctttattatt
     6541 atcggcttgt atgatgttag aggatcttca tcaggaaagt gttgctacaa aaattcgaaa
     6601 tgcattaaac aaaacatttg aagaaagaaa ggtattaact tttgatcttg gcggaagtgc
     6661 ttctacagat gcattcacgg acgaaattat taaaaattta taatataaaa gacgaagtat
     6721 ttactcttcg tcttcatctt cgtcaaccat tagagattga cgatttttta atgttaaaat
     6781 atatgttcca tcttttggat atgcactttt gtctttttga tcactcaaga tttccaattc
     6841 acatgggtat ccttcatttt caagaatttg taaaagattc tccttaccaa tttttttagt
     6901 aatagcacgt ccacacgcac agttaccata ttgatcaagg cattgtttaa tccacacagg
     6961 aatttttgaa gctgccattg tatcctcctt gattaaaagt attcgcaaaa tatatcatat
     7021 gcaaaacacc tttaaaaatc aagcagaatt cacattaacc ttctaaaact gcaataatat
     7081 ttggataata tgtaaaacga tcatctacaa attgtaaagc ttggtttgcg atttgtttcg
     7141 ccttgtcttt atccacacct tcataaatag aagcataacg atagtagata taatctttga
     7201 tttggttttc tcttaagata taaccataat ctgcatcacc taatccataa tctccatgta
     7261 agatatatag atcagctaca tccatcataa tacgatttct atatgtgtcg tgtagatctt
     7321 ctaattgtga taatacggca taaataaagt gttcataata atctacatct tcatatttcc
     7381 atgttttcaa aacgctatac gcaagatcca tgaattcttc aacgaatttt tcaacatcga
     7441 ttccgatttc ttcatctaaa ttttttgaat catgaatatc tttatgttgt gcaatcatta
     7501 atactttacg catcgtatca aattgagcct tgtattcttc tccatccgca acaacatctg
     7561 taaatttata attcaacgca tgatcaacat ccgcttgtag atcttcagct ttctgaggtt
     7621 taatatctgc tgtttcatat actttagata ctaaaacttc tactaaacct tcatcgtatc
     7681 catcttcgtc aaaattaaat ttacctgtta attgatactt attcactaaa tcttttgata
     7741 ctgattttac atctggattt aaaatcaatt gttgaataat agaatcacga gttacattca
     7801 ttgacatagc ttttgccaaa tcaagacatg tatctaatga taacttcgca tattgttctt
     7861 ctttagtcat tttcttagtc gttacctttt tagtagttgt ttttttagtt gtagctttct
     7921 ttgttgtttt tttagctgta gctttctttg tttctgcagg tttaacttct tcttttttag
     7981 ctttaactgc tgactttgta gctttctttg caggagcttt ctttgtttct gcaggtttta
     8041 cttcttcttt tttaacttca acagctggtt ttgcagcttt ctttgttgta gctttctttg
     8101 caggagcttt ctttgtttct gcaggtttta cttcttcttt tttagcttca actgctggct
     8161 ttgcagcttt ttttgttgta gctttttttg caggagcttt ctttgtttct gcagatttta
     8221 cttcttcttt tttagcttca actgctggtt ttgcagcttt ttttgttgta gttttttttg
     8281 caggagcttt ctttgtttct gcaggtttta cttcttcttt tttaatttca acagctggtt
     8341 ttgtagcttt ctttacttct gcagttttta cttcttcttt tttagcttca acagctggtt
     8401 ttgtttcttc ttttactact tttttatttg taactttttt tgtcgttttt attggcatat
     8461 gaaacctccc ttaaacgtgc tttctataac gttattatag cactgaatac aaatattccc
     8521 aaatttgtat agacaaaaag gtatatttga cgcatatatt taatgataaa tggtgtatac
     8581 tacagaatat agcatgcaaa aggaggaatt aacatgcaaa caccaccaaa atttaaacct
     8641 aaaacatatg ttcctgttcg aaataaaatt ctaaccactg caggtataat cttgttatct
     8701 ggtattgtcg gatttggtgc tggttatgcc ggaagtcatt tatctaataa caccaatgtc
     8761 accattcaac aacaaactaa taaatccaat tctggaacca ttcaagtagc agatgtttca
     8821 gatatcgttg aaaaatgtaa ggatagcgta gtagaaatca cgacggagtc tgtttctagt
     8881 ggaaactcta tcttcggcca atatgtgtct caaggtgcag gtagtggagt catcattagt
     8941 gaagatggat atattgtcac aaacaatcat gttgtgagtg gtgcaactag tttaaaagtc
     9001 acaacaacag atggaacaga atacgatgca tcgattattg gtacagattc gcaaaccgat
     9061 ttagctgtca ttaaaatcga agctcaaaat cttttagccg ctacattagg tgatagcgat
     9121 atcttacaag taggagatcc tgccattgcg attggaaatc cattgggtga acttggggga
     9181 acagttacta caggtattat ttctgcaacc gatagacaga tcacaatcga taacgaaaca
     9241 atgactttac tacaaacaga tgccgccatt aatccaggaa actctggagg tggattattt
     9301 aatgcagatg gcaatctaat tggtattgta aatgcaaaag aaagttctac aggaattgaa
     9361 ggattaggct ttgcgattcc aattacacct gccaaagatg tgattacaga acttatgcaa
     9421 aatggaagtg taacttctag gccagccttg aatgttagtt tatatgacta tacttcaagt
     9481 aaccaaacgc aaaattctaa atatgaagat ggctgttata tcgtgcaaat cgtaaagaat
     9541 ggagctgccg ataaagcggg attaaaacaa aatgaccgca ttatccgttt tgatggagaa
     9601 aagattcaat caacaagtga tgtaaaagct atattaaaaa agcataaaat tggagataac
     9661 gtcaagatgg ttgttgatag agattctaaa gaaatcgaag tcgaaattgt cttacaagca
     9721 caaacccaag caaattaagc cagtgattca ctggcttttt agtttaaact aacctataat
     9781 atacgtgtag atataagaag gagaatgaag ttatgtctga aaaaatcaaa aaaagaaaaa
     9841 tcatgcttgt tggtactggc tttgtcggca tgagttttgc ctattcaatg ctttctgaaa
     9901 aaggtattga tgaacttgtt ttagttgatg tgaatgaaga caaagcaaga ggagaacaaa
     9961 tggatcttag tgatggtctt gtatatgcag atacaaagat gaaaatcaca gctggaactt
    10021 atgcagatgc tgcggacaca aatatcgttg tcttaacagc tggagctgct caaaagccag
    10081 gtcaaactcg tttggattta gttaaaatca atgcaaatat cacaaaaggt gtatgccaag
    10141 acttaaaaga aaataacttt aatggtatct tagttgttgc aaacaaccca gtagatatta
    10201 tgacttatgt agcttggaaa gaaagtggat tacctaaaaa ccacgtaatc ggttcaggta
    10261 cagtattaga cacggctcgt ttacgtcatg cattaagtga aaaattagga tttgctgaca
    10321 gtaacatcca tgcttatatc atgggtgaac atggtgattc tagtttcgtt ccttggattc
    10381 actcatatat tggatgcaaa aatttacttg aatacttaga tgaaaataat atttctttaa
    10441 gtgaattaca agaaatctat attgatgtac gtgacaaagc ttataagatc atcgaattaa
    10501 aacgtgctac ttactacgga atcggtttgg cattaaaacg tatcgtatct tgtattttaa
    10561 ataatgaacg tgctatctta ccagttagtg catatcaaa
//
LOCUS       DBMDWL010000068        10270 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2278_length_10270_cov_3.271511, whole genome shotgun sequence.
ACCESSION   DBMDWL010000068 DBMDWL010000000
VERSION     DBMDWL010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_10270_cov_3.271511"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /gene="pulA"
                     /locus_tag="ACZPDN_05995"
     CDS             <1..1882
                     /gene="pulA"
                     /locus_tag="ACZPDN_05995"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HIR1298208.1"
                     /translation="YDEHCKTCVAKYSHIVKTERFAKEFTYEKDDLGVTYTPEKTIFK
                     VWAPTALSVNVGYVLNGKRIVESMVRQDKGVFSLTVNSDLNGVHYSYLVRVNGEYKGV
                     TDPYTCFSGPNAQYSVVIDPKSLNLPKKIKLKPMDSECDAIIYEASVRDMTSQTGIGI
                     SHPKKFVGFTEENEITKTHNTGFSYLKSLGVTHVQLMPVFDFGSVDEVYPNIFYNWGY
                     DPVQYRCLEGAYSLDPANAKLRIEEFAKLVHDCHKAGIRVNLDLVFNHVYVKENFALE
                     NMVPDYYFLMNREGEFSNGSFCGNDIDTKPYMARKYFIETCKKIIEMYDVDGFRFDLM
                     GILDFNLMNEISEECKKVKPDFMIYGEGWNMPSFVAEEIRASQLNQAKMPHVGHFSDR
                     FREVVRGSNDELSRKGFASGQADLIYQVKCCMAASCLDHVFDSPTKVVNYVECHDNHT
                     LWDKNRVCCHGESRDLREKRQILANAMVLLAQGIPFIHCGQEFGRTKQNLGNTYNRSD
                     NYNRVDYQRRDHHIEIVERTKELIEIRKTHPCFRLSTVEQIEQGVQFDTIYDQVLVYS
                     CQKGKDHCVAFFNPTNIPYDYHLDQEARILFDNGNSNSLDTFDIHIAAFSVVVCQFGL
                     TA"
     gene            1928..3439
                     /locus_tag="ACZPDN_06000"
     CDS             1928..3439
                     /locus_tag="ACZPDN_06000"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1298209.1"
                     /translation="MAKFFEEPSRTFSEYLLIPGYTGPDCTPDKVSLKTPLVKFKKGE
                     EPALSLNIPMVSAVMQAVSGEEMACALAREGGVSFIYGSQTIESQAAMVRKVKSTKAG
                     FVGSDSNISPEATLEDVIALRKETGHSTMAVTDNGQADGKLVGIVTSRDYRESRMDHS
                     IQVKEFMTPFDKLIVGNEDITLSDANDLIWEHKLNQLPIVDKEQHLKAFVFRKDYESH
                     KDNPNELLDENKRYIVGAGINTRDYATRVPALVEAGVDVLCIDSSEGYSAWQAETIKW
                     IREHYGDRVKVGAGNVVDGDGFRFLADAGADFIKIGIGGGSICITREQKGIGRGQATA
                     TIDVAKARDAYFEETGVYIPICSDGGIVHDYHITLALAFGADFVMLGRYFARFKEAPN
                     KIVSVNGNYMKEYWGEGSARARNWQRYDVGGDKKMSFVEGVDSYVPYAGSLRDNVGLS
                     LSKIRHTMCNCGCLTIPELQRDAKVTLVSSTSIVEGGAHDVVVRDEHFDAKPQ"
     gene            3495..4637
                     /locus_tag="ACZPDN_06005"
     CDS             3495..4637
                     /locus_tag="ACZPDN_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1298210.1"
                     /translation="MEKQNDLLMDSSILYRSTQKYYDKMLQDLNLSYAQLPILIEIYE
                     NEGISLQQIVQVGGYDKGTVTKNVQKLNTLGYVSILTSAKDKRAKELYTTALTKKHIS
                     EIYGIRRDWWHHITQDLNADEIEVFSKFYQTLSNHARSYADLEKTHLQFYKLKKLSLS
                     DFDPYLSCSLYTGGCNLKCPYCHSKDLVYLKENMYPIANEKISEYLKSHHKDLEGIYI
                     SGGEPLMHEGILSFLQYAKSLDYKIKLHTNGMFYDRLKRILEDGLVDYVSLDIKNAPS
                     AYAKTCGVEDVDLMDIEKSLNELKASSFDYDIYITLVDEFHNEKTIDELGQWIQGVHH
                     VVLQPFKDCQTTIDHSLHSPNFDQILKYKTILEQYVKHVEIRGNQT"
     gene            4634..6592
                     /gene="nrdD"
                     /locus_tag="ACZPDN_06010"
     CDS             4634..6592
                     /gene="nrdD"
                     /locus_tag="ACZPDN_06010"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1298211.1"
                     /translation="MIEVEKRDGSIVDFDPSKIFSAISKAFDSLHMEKDDAIINLLVL
                     RATADFQAKIKENRISVEMIQDSVEKTLSESGYYPVAKTYILYRKQRENVRKIQSTAN
                     EYIHLVNSYLHNNVPLEDENSLATYSVGGLILSNSGMITSNYWLSEVYDAQITNAHKN
                     GDLYIHNIHMLTGCSAGWSLEDLILHGLPSVNKMISSLPAKHLSTLCNQMVNFLGIMQ
                     NEWASAQSLAHFDTLLVPFIHKDKLSKKMVYDCLESFIYGINIPSRWGTQAPFSQITL
                     DWNIPKEFLNRKAIVAGLEEDFTYADCQVEMKILHDALFEVLNKGDISGRGFQFPIVA
                     LYLHENFDWMQEEELFKACAKYGTPYFLTQEKQDVEGYFGYKPLCGSMGVVTLNLVRL
                     AYLSNSKDDFFKRLDSLSDVAIRSFEVKRQVLNQLFKAGLYPYTKAYISDFNDYYGTL
                     GVVGMNEACLNAKWLQKDLMDMHAQKFAQKVLNFLNQKISNQAQKLNLEATPAESVCT
                     HFAKIDKEKYPEIQSFGYYTNSTHLDVASTDDVFEALRIQQKVQTLYSGATSFPVFMD
                     HGIENWKMAALLVKTIYENYNIPVFTITPTYSVCTDHGYVFGKQDVCPICQKPLEIYS
                     RVSGYYRNLEDWNEGKQKEFSRRKTYSI"
     gene            6603..7409
                     /gene="proC"
                     /locus_tag="ACZPDN_06015"
     CDS             6603..7409
                     /gene="proC"
                     /locus_tag="ACZPDN_06015"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR1298212.1"
                     /translation="MRETIGFIGAGNMGGAMIEGIAKNKLFNTIWVCDHHQENLDRLH
                     EKYGVNTSLDEKTVALNSDVLVLSVKPKHYPKLIETIKECVKSNVIIVDIAAGVTIRD
                     VKHYFGKDLKVIKAMPNTPALVLQAMSAISFDDLVSEEDKTCITSIFNSFGKCEVVDE
                     TMMDAITSVSGSSPAYVFMFIEAMADEAVAQGLPRKQAYIFAAQAVLGSAQMVLETGM
                     HPGALKDMVCSPGGTTIDAVCELERTGFRASVESAMRVCNEKSKKMTEGK"
     gene            7412..8701
                     /locus_tag="ACZPDN_06020"
     CDS             7412..8701
                     /locus_tag="ACZPDN_06020"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HIR1298213.1"
                     /translation="MKNICIGLFEGETLPESLAAFKDVKLDLEFGKITTIHTLNQLDC
                     EKIVVVGLGDGKKNIKEAFSKVEADDYFVYVNENTAYAAGFGLVYGAYSYKETKVYDF
                     ESDGFTAEFEKGKTVALIVNHAREMSDMPANFLTPDHLVDEAKMVSEKYGMEIEVLNH
                     DDLEQLGAGALLAVNQGSDRPCYMVVIRYDGGKEEEEYTALVGKGLTYDAGGYNIKSN
                     MHGMKYDMCGAANMLCALECMAELKIEKNIVCVLPITENKINGHGFVDGDVITSLSGK
                     TIEVTNTDAEGRLILADALTMAQKVGATRVIDMATLTGACVRALGSTYTGIFSNDDSF
                     YLPFLGASKATKEQIWRLPVDEYFHSELKCSKVADIVNSKTLGGKASLAAAFLEEFIE
                     EGTKWIHLDIAGTSDKDEVATGVMIETIVHFFENECK"
     gene            8771..9832
                     /locus_tag="ACZPDN_06025"
     CDS             8771..9832
                     /locus_tag="ACZPDN_06025"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR1298214.1"
                     /translation="MEVQFVESKNLKEKPTGALGFGKYYTDYMFVMDWDAGQEWHDAR
                     VVPYGPLEFDPASMVLHYAQETFEGMKAYRTKDGKVQLFRPEMNAKRFANSNKRLSMP
                     TLPVDMFVDAVKTIVKYSQDWIPTGEGESLYIRPFMFATEAAIGVHAASHYKFMIICS
                     PVGAYYAEGVNPVKIYVEDEYVRATKGGTGFTKCGGNYAGSLAAQVKAEELGYSQVLW
                     LDGVHRKYIEEVGSMNVMFEIDNKIVTAPCEGTVLPGVTRDSILHILKDWGYEIEERH
                     LSVDELMEAGHNGKLQEAWGTGTAAVISPIGELYYKGDKVTVSDFKTGELTQKLYDTL
                     TGIQWGTIEDPYGWTVVVD"
BASE COUNT         3371 a         1351 c         2271 g         3277 t
ORIGIN      
        1 atatgatgaa cattgtaaga cttgtgtcgc caaatattct catattgtca aaacagagcg
       61 ttttgcgaaa gagtttacgt atgaaaaaga tgatttaggt gtgacatata cacctgaaaa
      121 aactattttt aaagtatggg caccaacagc tttatctgtc aatgttggat atgtcttgaa
      181 tggtaagaga atcgttgaat cgatggttcg acaagataag ggtgtttttt cacttactgt
      241 taattcagat cttaatggtg tacattactc ttatttagtt cgagtcaatg gtgaatataa
      301 aggggtaaca gatccatata cttgttttag tggtcccaat gcacaatata gtgtggtgat
      361 agatcctaaa agtttaaatc ttccaaaaaa aatcaaattg aaaccaatgg atagtgaatg
      421 tgatgcgatc atatatgaag caagtgttcg tgatatgact agccaaacag gaattggcat
      481 atcacatcct aaaaagtttg tgggatttac ggaagaaaat gaaatcacaa agacccataa
      541 tacagggttt agttatttaa aatcattggg cgttacacac gtacaattga tgcctgtttt
      601 tgactttggc agtgtcgatg aagtatatcc aaatatattc tataattggg gatatgatcc
      661 ggtgcaatat cgttgtttag aaggtgctta ttctctagat cctgccaacg caaaattaag
      721 aattgaagaa tttgcgaaac tcgttcatga ttgtcataag gcagggattc gtgtcaattt
      781 agatttggtg tttaatcatg tatatgtgaa agaaaatttt gcacttgaaa atatggttcc
      841 agattattat ttcttaatga atcgggaggg tgaattctcg aatggtagtt tttgtggaaa
      901 tgatattgat acgaagcctt atatggcacg taaatatttt attgaaacat gtaaaaaaat
      961 cattgaaatg tatgatgtgg atggattccg ttttgatcta atgggaattc ttgattttaa
     1021 cttgatgaat gaaattagtg aagagtgcaa aaaagtaaaa cctgatttta tgatttatgg
     1081 agaaggttgg aatatgccaa gttttgtggc tgaagagatt cgtgcttcac aattgaatca
     1141 agctaagatg ccacatgtag gtcatttctc ggatcgtttc cgtgaagtag ttcgtggatc
     1201 aaatgatgaa ctttcacgta aaggatttgc ctcaggtcaa gcggatttga tctatcaagt
     1261 gaagtgttgt atggcagcta gttgtttaga tcacgtattc gatagtccta caaaagtagt
     1321 caactatgta gaatgtcatg ataatcatac gctttgggat aaaaaccgtg tatgttgtca
     1381 tggagagagt cgtgatttaa gagagaaacg tcaaatactt gcgaatgcga tggtattact
     1441 tgcgcaagga attccattta ttcattgtgg gcaagagttt ggtcgtacca aacaaaattt
     1501 aggcaacaca tataatcgtt ctgacaatta taatcgtgtg gattatcaaa gaagagatca
     1561 tcatattgaa attgttgagc gtacaaaaga attgattgaa attcgtaaaa cacacccttg
     1621 ttttagatta agtacggttg agcagattga acaaggtgtt caatttgata cgatatatga
     1681 tcaggtctta gtctattctt gtcaaaaagg caaagatcat tgtgtagctt tctttaatcc
     1741 aacaaatatt ccatatgatt atcacttgga tcaagaagct cgaatcttgt ttgataatgg
     1801 caacagcaat tctttagata cgtttgatat tcatattgca gcatttagtg ttgtcgtttg
     1861 tcaatttggc ttgacggctt agaacgcaaa acatataata tagtttaaat tgagaaggag
     1921 atataaaatg gcaaaatttt ttgaagaacc atctagaacg ttcagtgagt atttattgat
     1981 tcctggttac acaggtccgg attgtacacc ggacaaggtg agtttaaaaa cgcctttagt
     2041 taaattcaaa aaaggtgaag aacctgcact ttctttaaat attccaatgg taagtgctgt
     2101 tatgcaggct gttagtggtg aagaaatggc ttgtgcactt gcacgtgagg gtggagtaag
     2161 ttttatttac ggtagtcaaa caattgagtc tcaagccgct atggttcgaa aagttaagtc
     2221 gactaaggct gggtttgttg gcagcgattc aaatattagc cctgaagcaa cattagagga
     2281 tgtaattgct cttcgtaaag aaacaggaca ttcaacaatg gctgttactg acaatggtca
     2341 agctgacggt aaattagtag gtatcgtaac aagtcgtgat tatcgcgaaa gtcgtatgga
     2401 tcattccatt caagtgaaag aattcatgac accattcgat aaattgattg ttggaaatga
     2461 agacattaca ttatcagatg caaacgattt gatttgggaa cacaaattaa atcaattgcc
     2521 tattgttgat aaagaacaac atttaaaagc ttttgtgttt agaaaagact atgagtcaca
     2581 caaagataat ccaaatgaat tattggatga aaataaacgt tatatcgttg gtgctggtat
     2641 caatacacgt gactacgcga ctcgtgtacc tgcattagtt gaagcaggtg ttgatgtgtt
     2701 atgtattgat tcaagtgaag gatattcagc atggcaagcc gaaacaatca aatggattcg
     2761 tgaacactat ggtgatcgtg taaaagttgg tgctggtaac gttgttgatg gagatggttt
     2821 ccgtttctta gccgatgcag gtgcagactt tattaagatt ggaatcggtg gaggttcaat
     2881 ctgtattaca cgtgaacaaa aaggtattgg tagaggacag gccacagcta caattgatgt
     2941 cgcaaaagct cgtgatgcat attttgaaga aacgggagtt tatattccaa tttgttcaga
     3001 tggtggtatc gtacatgatt atcacattac attggcattg gcatttggag cagacttcgt
     3061 tatgttaggt cgttactttg ctcgttttaa agaagctcca aacaaaattg taagtgtgaa
     3121 tggaaattat atgaaagagt attggggtga aggtagtgct cgtgctcgta actggcaaag
     3181 atatgatgtt ggtggagaca agaaaatgtc ttttgtcgaa ggtgttgatt catatgttcc
     3241 ttatgcagga agcttaagag acaatgttgg tttatctctt tctaagattc gtcatacaat
     3301 gtgtaactgt ggatgtttga caattccaga acttcaaaga gatgcgaaag tcactttagt
     3361 atctagtaca agtattgtcg aaggtggtgc tcacgacgtt gttgttcgtg atgaacattt
     3421 cgacgcaaaa ccacaataat tccaaaagga cttcggtcct ttttttcttt ataaaaatcg
     3481 cttataattc ctatatggaa aaacaaaacg atttattgat ggattcaagc attctatatc
     3541 gaagtacaca aaagtattat gataaaatgc ttcaggattt aaatttaagt tatgcgcagc
     3601 tgcctatttt aattgaaatc tatgagaatg aagggatttc tttgcagcaa attgtacaag
     3661 ttggaggcta cgataagggt actgtgacta aaaatgttca aaagctgaat actttaggct
     3721 atgtttctat tttaacaagc gcaaaggaca agcgtgcgaa agaattatat acaacggcac
     3781 ttacgaaaaa acatatttct gaaatatatg gaattcgaag agattggtgg catcatatta
     3841 cgcaagattt aaatgcggat gaaatagaag ttttttcaaa gttttaccaa acgttatcca
     3901 atcatgcgcg tagttatgcg gatttagaaa agacgcatct tcaattttat aaattaaaaa
     3961 aattatcctt atcggacttt gatccgtatt taagttgttc gttatatacg ggtggatgta
     4021 atttaaagtg tccatattgt cattcaaaag atttggtgta tttaaaggaa aatatgtatc
     4081 cgattgccaa tgaaaagatc agtgaatatt taaagagtca ccataaagat ttagagggaa
     4141 tttatatatc gggtggggaa cccttaatgc atgagggtat tctatctttt ttacaatatg
     4201 ccaagtcact agactataaa attaagcttc atacaaatgg gatgttctat gatcgtttaa
     4261 agcgaatctt agaagatgga ttggttgatt atgtgagttt agatataaaa aatgcgccga
     4321 gtgcctatgc gaaaacatgt ggtgtagagg atgtggatct aatggatatt gaaaagtcat
     4381 tgaatgagtt aaaagcatct tctttcgatt atgatattta tatcacattg gtcgatgaat
     4441 ttcataatga aaagacaatc gatgaattag gacaatggat tcaaggtgta caccatgttg
     4501 tgttacagcc ttttaaagat tgtcaaacaa ctatcgatca ttctttgcac agtccaaatt
     4561 ttgatcagat tttaaaatat aagacaatat tggaacaata tgtaaaacat gtagagataa
     4621 gggggaatca aacatgatag aagtcgaaaa aagggatggg tccatagttg attttgatcc
     4681 atcaaaaatc ttttctgcga tctcaaaagc ctttgattct ttacatatgg aaaaagatga
     4741 tgcgatcatc aacttgttgg tgttgcgtgc tacggcagat tttcaagcga agatcaaaga
     4801 aaatcgaatc agtgtcgaaa tgattcagga cagtgttgaa aaaacgttgt cagaatctgg
     4861 gtattatccc gttgcgaaaa cgtatatttt gtatcgtaaa caaagggaaa atgtacgtaa
     4921 gattcaatcg acagccaatg aatatattca tttagtgaat tcatatttgc ataataatgt
     4981 acctttagaa gatgaaaact cattggctac ttattctgtg ggtggtttaa ttctttctaa
     5041 ttcaggtatg atcacatcaa attattggtt aagtgaagta tatgatgcgc aaattaccaa
     5101 tgcccacaaa aatggagatc tatatattca taatattcat atgttgacag gatgtagtgc
     5161 tggatggtct ttagaagatt tgattttaca tggtcttcct tctgtgaata aaatgatttc
     5221 ttctttacca gccaaacact tgagtacatt atgtaatcag atggtgaatt ttttgggtat
     5281 tatgcaaaat gaatgggcaa gtgcacaatc tttagctcat tttgatacgt tattagtgcc
     5341 ttttatccat aaggataaat tgtcaaaaaa gatggtatat gattgtttag aaagctttat
     5401 ttatggaata aatatccctt ctagatgggg aacgcaagct ccttttagtc agattacttt
     5461 ggattggaat attccaaaag aatttttaaa cagaaaggcg atcgtagccg gtttggaaga
     5521 agattttaca tatgcagatt gtcaagttga aatgaaaatc ttgcatgatg cactctttga
     5581 agttttgaat aaaggcgata tatcgggtcg tggttttcaa tttcccattg ttgcccttta
     5641 tctacatgaa aactttgatt ggatgcaaga agaagagtta tttaaagctt gtgcaaaata
     5701 tggaacgcct tattttttaa cccaagaaaa acaggatgta gaggggtatt ttggatataa
     5761 acctttatgt ggcagtatgg gtgttgtcac tttaaatctc gttcgattgg cttatttatc
     5821 aaactcaaaa gatgatttct ttaagcgttt agatagcttg agtgatgtag ctatacgttc
     5881 ttttgaagtg aaaagacaag ttttaaatca attgtttaag gcgggactct atccatacac
     5941 aaaagcttat atatctgatt ttaatgatta ttatggaact ttgggcgtcg tgggcatgaa
     6001 tgaggcttgt ctgaatgcga agtggctaca gaaagattta atggatatgc atgcacagaa
     6061 gtttgcgcaa aaggttttaa attttttaaa tcaaaagatt tcaaaccaag cgcaaaagtt
     6121 aaatttagag gcaacacctg cagaaagtgt ttgtacgcat tttgcgaaaa tagataaaga
     6181 gaagtatcca gaaattcaaa gttttggata ctatacgaat tcaacgcatt tagatgttgc
     6241 cagtacagat gatgtatttg aggctttgcg tatacaacaa aaagtacaaa ctctttatag
     6301 tggggctact tcttttccgg tttttatgga tcatggcatt gagaattgga aaatggcggc
     6361 gctattggta aaaacaattt atgaaaacta caacatacct gtctttacaa ttacgccaac
     6421 ctattcggtt tgtacagatc atggatatgt atttggaaaa caagatgttt gtccaatatg
     6481 tcaaaaacca ttagaaatct attcaagagt atcgggttat tatcgtaatc ttgaggattg
     6541 gaatgagggc aaacaaaaag agttttcaag aagaaagaca tattcgattt aaggagctaa
     6601 cgatgagaga aacaattgga tttattgggg ctggaaacat gggtggtgcc atgattgaag
     6661 ggattgccaa aaataaatta tttaatacga tttgggtgtg tgatcatcac caggaaaatt
     6721 tggatcgttt acatgaaaag tatggtgtta atacttcttt agatgaaaaa acagtcgctt
     6781 taaatagtga tgtattggtt ttgagtgtta agcctaagca ttatcctaaa ttgattgaaa
     6841 cgatcaaaga gtgtgtaaaa tcaaatgtga tcattgtcga tattgcggcc ggtgttacaa
     6901 ttcgtgatgt gaaacattat tttggaaaag atttaaaagt tattaaagct atgcctaata
     6961 cgccagcttt agttcttcaa gctatgagcg ctatttcttt tgatgattta gtgagcgaag
     7021 aagataaaac ttgtatcaca tcgatcttta atagttttgg aaaatgtgaa gtggttgatg
     7081 aaactatgat ggatgccata actagtgtta gtggttcaag tcctgcttat gtatttatgt
     7141 ttattgaagc tatggctgat gaagcagtag cgcaaggatt gcctcgaaaa caagcttata
     7201 tatttgcggc acaagctgtt ttaggaagtg cacaaatggt attagagaca ggtatgcatc
     7261 caggtgcatt aaaagatatg gtatgttcgc ctggtggaac aacgattgat gcggtatgtg
     7321 aactggaaag aacaggattt agagcaagtg tagagtcggc aatgcgtgtt tgtaatgaaa
     7381 aatcaaagaa gatgacggag ggaaaataga catgaaaaat atttgtatcg gattatttga
     7441 gggggaaaca ttaccggaaa gtttagcggc tttcaaagat gtgaaattgg atttagaatt
     7501 tggtaagatc acaacgattc atacattgaa tcaattggat tgtgaaaaga ttgtagtcgt
     7561 tggtttgggt gatggtaaaa agaatatcaa ggaggctttt agtaaagtcg aagctgatga
     7621 ttatttcgta tatgtcaatg aaaatacagc gtatgcggct ggctttggac ttgtatatgg
     7681 tgcgtatagt tataaagaga caaaggtgta tgattttgaa agtgatggat ttacggctga
     7741 gtttgaaaaa gggaaaacag ttgctttaat tgtgaatcat gcgcgtgaaa tgagtgatat
     7801 gccagctaac tttttaacac cggatcattt ggtggatgag gcaaagatgg ttagtgaaaa
     7861 gtatggtatg gaaattgaag tcttaaatca tgatgattta gaacaacttg gtgcaggtgc
     7921 acttttagcg gtcaaccaag gttcagatag accttgttat atggttgtga ttcgttatga
     7981 tggtggaaaa gaagaagagg agtatacggc actcgttggt aagggtctta cttatgatgc
     8041 gggtggctac aacatcaaat cgaacatgca tggtatgaaa tacgatatgt gtggagcggc
     8101 taatatgctt tgtgctttag agtgtatggc tgaattaaaa atagaaaaga atattgtgtg
     8161 tgttttacca atcacagaaa ataaaatcaa tggacatggc tttgtcgatg gagatgtgat
     8221 cacaagctta agtggtaaga cgattgaagt cacaaatacg gatgcagaag gaagattgat
     8281 tttggccgat gccttgacaa tggctcaaaa agtaggggca acacgcgtaa tcgatatggc
     8341 aactttaacg ggtgcttgtg ttcgtgcatt aggaagtaca tatacgggaa tttttagtaa
     8401 tgatgatagt ttctatttgc ctttcttagg ggctagtaaa gctacaaagg aacagatttg
     8461 gcgtttgcct gtagatgaat attttcattc ggaattaaag tgttctaaag ttgcagatat
     8521 tgtcaactca aagactttgg gtggaaaagc aagcttggct gcggctttct tggaagaatt
     8581 tattgaagaa ggaacaaaat ggatccactt agatattgcg ggaacaagcg ataaggatga
     8641 agtggctaca ggtgtcatga ttgagacaat tgttcatttc tttgaaaatg aatgtaaata
     8701 aatgaaaata tattgaaata aattacagaa cgtggtatca tcatacgtgt ttagaaaaga
     8761 ggacaaattt atggaagtac aatttgtaga aagtaaaaac ttaaaagaaa aaccgaccgg
     8821 agctttaggt tttggtaaat attacacaga ctatatgttt gtgatggatt gggatgctgg
     8881 tcaagaatgg catgatgcaa gagtcgtacc ttatggacca ttagaatttg atccagcaag
     8941 catggtattg cactatgcac aagaaacatt tgaaggtatg aaggcatatc gtactaagga
     9001 tggcaaggtt caattgttta gaccggaaat gaatgcgaag cgttttgcga attcaaataa
     9061 acgtttgagt atgccaacat taccagtgga tatgtttgta gatgcagtaa agacaattgt
     9121 aaaatattct caagactgga ttccaactgg agaaggcgaa tcattatata ttcgtccatt
     9181 catgtttgcg acagaagcag cgattggtgt acatgctgca agtcattata aattcatgat
     9241 tatttgttca cctgttggtg cttattatgc cgaaggtgtg aatccagtta agatttatgt
     9301 tgaagatgaa tatgttcgtg ctaccaaagg tggtactgga tttacaaaat gtggtggaaa
     9361 ctatgcgggt agtttagctg ctcaagtaaa ggctgaagaa ttgggttatt ctcaagtatt
     9421 atggttggat ggagttcacc gtaaatatat tgaagaagtt ggttctatga acgttatgtt
     9481 tgaaattgat aataagattg tgacagctcc atgtgaaggt acagtacttc ctggtgttac
     9541 tcgtgattct attttacata tcttaaagga ttggggatat gaaattgaag aaagacattt
     9601 atctgtcgat gaattgatgg aagccggtca caatggtaag cttcaagaag cttggggtac
     9661 tggtactgca gctgttatta gtcctattgg tgaattgtac tacaagggtg ataaagtgac
     9721 tgtttcggat ttcaaaacag gtgaattaac acaaaaatta tatgatactt taacaggtat
     9781 tcaatgggga actattgaag atccttatgg atggactgtt gtcgttgact aattaatgaa
     9841 ggtgtacatt gtgcatcttc tttttttgtg aaagttgatg tgtaataatg caagtttgaa
     9901 gtttaattta acttaaaaat tgcatctcaa cttaaaaaga gtaaagatac agaaaaagag
     9961 ttaaaaatgg tggtgaacat atgaagtatc tcaataaagt tcttgggatt gaagagattt
    10021 agaatgcaat ttaagaacat ttttgacttt aatcttgaga tgatttagat gtgtggacgt
    10081 aagatcaata aatttaaaat aattttgatt tttggttttc acaataaaaa aatagagatt
    10141 tctctctatt cttgttcata tttttttaga gtgtattccg tatgcatctt atagatgata
    10201 cattgaatga cattgtccat aagttcttgt tcatggttgg cgttttcaaa ttcgatggta
    10261 ttacggttat
//
LOCUS       DBMDWL010000069        10046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2353_length_10046_cov_3.115435, whole genome shotgun sequence.
ACCESSION   DBMDWL010000069 DBMDWL010000000
VERSION     DBMDWL010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_10046_cov_3.115435"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="ACZPDN_06030"
     CDS             complement(<1..418)
                     /locus_tag="ACZPDN_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298215.1"
                     /translation="MNHEEIIARMAEIEREIAVLPAGGVSKKNVHGNIYYYHRITQNG
                     KRMEKYVKADEVDELISNIEKRKKLEKELKKLKQCMPKEKNLDFKTKVLVGDRLKSLV
                     SITRNYKKRECIQMLRDYIFEDIADKVFILYGLRRTG"
     gene            complement(495..1847)
                     /locus_tag="ACZPDN_06035"
     CDS             complement(495..1847)
                     /locus_tag="ACZPDN_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298216.1"
                     /translation="MKALESTDLLKGSIWKSILIFSLPLLVGNLFQQLYNTVDSYVVG
                     NFVSSHALAAVGQSTSIINMLVGFFMGLSTGAGVVIAQYFGAKETKKMQDSIHTSLAL
                     TLVLCVLFTILGIALSKPILVMIGSPKEVLPLAVIYLQIYFAGVSFSLIYNMGAGILR
                     ALGDSKKPLIYLVVSSLVNIILDFAFVIYFHLGVAGVGIATTLAQLVSAILVMHELMH
                     TDKEYKVYISKIRFSKPILSRIISIGIPAALQNSIVSFSNVIVQSYISKFGSAAVAGY
                     STTTKLDGFLQLPIQSFSMAITTFVGQNYGAQNYKRVRKGLYTTLLMCEVIIALGAFL
                     IYTNGEFLVGLFSQDKDVIVAGVTMISVFAPGYIFLPLSHITAGALRGVGLSKIPMYS
                     MILCFVILRQVYLFVATQFSSELITVFLGWPLTWIVNAALLMGYYHIYSKKLVRGDIY
                     "
     gene            complement(1934..2686)
                     /locus_tag="ACZPDN_06040"
     CDS             complement(1934..2686)
                     /locus_tag="ACZPDN_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1298217.1"
                     /translation="MIMWLGLLIPFFGTTLGSAVVYFMENKNNELLSRCFSGIASGVM
                     IAASIWSLLIPAIEQEKNILIIVLGIFLGALLLLALDCIVPHMHPGTNDEEGKESHLK
                     KTTKLVFAVTLHNIPEGMAVGVVLAQALNTQNLYSALALSIGIAIQNFPEGAILSLPL
                     KSNGYSKHKAFLIGALSGIVEPIFGYITILFSNWILQCLPLCLSFAAGAMLFVVIEEL
                     VPEMAQGKHSNIPTLSFLFGFCVMMVLDVILG"
     gene            complement(2723..3895)
                     /locus_tag="ACZPDN_06045"
     CDS             complement(2723..3895)
                     /locus_tag="ACZPDN_06045"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF014600.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1298218.1"
                     /translation="MKKVKRKVNRKRIIIVVLIPLICIALLIANFTSIRLSIKGYHKE
                     DKKVVLKLETDDIKDILDYDQIIDISKWNKVKNDSHYLLYDKYYRASKESVKKVVYYI
                     DSYYERMEDLNYLGYTTDVLFNNSDIYTISNLDTLISASVPYKKAKKYLAIKGAQIKD
                     IKEYVDSGLKPLKAVLTVSYPGIDSSKRGDRTYTIEDPANMLVLIKNGFSVPSDYVPS
                     DLRDVNIPYESEVGQLRDEAATALENMYKDGLKKGYSIAIKSSYRSYETQLAVYNEYF
                     AMYDAAYAASLVSTPGSSEHQCGLSVDLTSQSVLDGEYGTFGESPDYDWVEENAYKYG
                     FILRFPENAGDRTGATNEPWHFRYVGKEAAKEIHDKGWILEDYIEHHGFAYNLRLN"
     gene            4010..5131
                     /locus_tag="ACZPDN_06050"
     CDS             4010..5131
                     /locus_tag="ACZPDN_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1298219.1"
                     /translation="MLFKNATIYTMEQDPFVGDFRIDKGVFTEVGKDLNPKDEEVQDL
                     NGLFVFPGLVESHCHLGMEETAIRFEGDDVNEITDPITPNMRGIDGCNPMDETVELAL
                     KGGVTTVAAGPGSANVLGGTFFAYKTKGNCIDEMTIQNPIAMKAAFGENPKRCYQGKS
                     IDTRMQISALLRETLEKTKEYMKKKESGKDVAYDQKLEAMIPVIKKELPLKCHAHRAD
                     DILTVIRIAKEYDIEVTLDHATDARCIVEQIKESGFPCICGPSFGHKTKFELKSKSFK
                     TPGVLNKAGILVSITTDSPVIPEQYLSLCAALAAKEGMDELEAIKAITINPAKILKLD
                     DRIGSIKSGKDADFIICTKNILDTTNEIKAVYVDGKKAV"
     gene            5131..6252
                     /locus_tag="ACZPDN_06055"
     CDS             5131..6252
                     /locus_tag="ACZPDN_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1298220.1"
                     /translation="MLFKNATIYTMEQDPFVGDFKIDKGVFTEIGKDLSPKDEEVQDL
                     NGLYVFPGLIDAHSHLGMVCSSIGFEGEDGNEVTDPVTPNIRGIDGCNPMDETIEEAL
                     KSGVTTVAAGPGSANVIGGTFFAYKTAGHCIDEMTIQNPLAMKAAFGENPKRCYKGKK
                     IDTRMQISALLRETLAKTKEYIQKKESGKDVDYDQKLEAMIPVIKKQMPLKCHCHRAD
                     DILTVIRIAKEYDIEVTLDHVTDGRSIIPQIKESGFPCILGPCLGHKSKYEVKNMSFE
                     TANEFYKAGIPFCIITDSPVVPEQYLSLSAALAHKAGLPEYEAIKAITINPAKILKLD
                     DRIGSIKVGKDADFVVCTKNILDTQNEIKTVYVNGKKEA"
     gene            6252..7376
                     /locus_tag="ACZPDN_06060"
     CDS             6252..7376
                     /locus_tag="ACZPDN_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1298221.1"
                     /translation="MLFKNATIYTMEQEPFVGDFKIDKGVFTEVGKNLTASEGEDVQD
                     LNGLYVFPGLVESHCHLGMEETAIRFEGDDVNEITDPITPNMRGIDGCNPMDETIESA
                     LKGGVTTVAAGPGSANVLGGTFFAYKTKGNCIDEMTIQNPIAMKAAFGENPKRCYKDK
                     KIDTRMQISALLRETLEKTKEYMKKKEDGKDVAYDQKLEAMIPVVKRELPLKCHAHRA
                     DDILTAIRIAKEENIKITLDHVTDARSILPQIKESGFPCICGPALTHKSKFELANMSF
                     ETPNELYKAGILFSIITDSPVIPQQYLSLSAALATKAGLPEYEAIKAITINPAKILGL
                     DNRVGSIKVGKDADFVICTKNILDTQNEIQFVYVDGKKAA"
     gene            7376..8497
                     /locus_tag="ACZPDN_06065"
     CDS             7376..8497
                     /locus_tag="ACZPDN_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1298222.1"
                     /translation="MLFKNATIYTMEQDPFVGDFRVNKGIFTEIGKNLEPKDEDVQDL
                     TGFNVYPGMVEAHCHLGMEETAIGFEGNDLNEITNPITPNMRGIDGCNPMDETVQTAL
                     EAGITTVAAGPGSANVIGGTFFAYKTKGNCIDEMTIQNPIAMKAAFGENPKNCYKGKN
                     IDTRMQISALLRETLAKTKEYMMKKETGKDVDYDQKLEAMIPVIKKEIPLKCHAHRAD
                     DMLTAIRIAKEYDIEITLDHATDSQAILDQIKESGFPCICGPSLCHKDKFECANDSFE
                     TPNKMYEKGILFAITTDSPFNPQQYLPINAALAHKAGLPENEAIKAITINPAKILHLE
                     NRVGSIKTGKDADFIICTKSLIDLNNTIKNVYINGIKAV"
     gene            8544..9728
                     /locus_tag="ACZPDN_06070"
     CDS             8544..9728
                     /locus_tag="ACZPDN_06070"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1298223.1"
                     /translation="MNIVEKYGGTSVGTIEQIQAIALHAKHMKEQGHNLVIVASAMGK
                     TTNKLIELAHSVTNNPNKRELDSLLSTGEQQTITLLAIAMNALGVDAISLTGYQAGFL
                     TSKHHSRALIKDIDTTTIQSHLDQGKVVVVAGFQGATEYGDITTLGRGGSDTTAVAIA
                     AKLNYQCHIFTDVAGVYTIDPRLYTRAKKLKSISYEEMMQMACLGAGVLETRSVELAS
                     KYNVPLFLGRALETDLEKGTWIMHKELEDMPITGLSVKDDYAIMRFMHLPNDGVYLGK
                     LFKMISDLNINLDTISQQLDDEGLANFTLYCSEEQSNQIMENASKEYPVHQMLGYIKL
                     SLVGLGISTHSGIASKVLNILSENNIKYYMITSSEISISLTIEKKDKVKAVQALGKAF
                     DL"
     gene            9728..9952
                     /locus_tag="ACZPDN_06075"
     CDS             9728..9952
                     /locus_tag="ACZPDN_06075"
                     /inference="COORDINATES: protein motif:HMM:NF028435.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298224.1"
                     /translation="MELPVKFKEQMKGLLQDEYEDYLQSFDHERYYGLRINTLKISVE
                     EFLKISPFKLKPIPWTNDGFYYSSEDKPSK"
BASE COUNT         3570 a         1781 c         1677 g         3018 t
ORIGIN      
        1 ttccagttcg tcttaatcca tacaatataa acactttatc agctatgtct tcgaagatat
       61 aatctctaag catttgaata cattctcttt ttttatagtt tcgtgtaata gaaactaaag
      121 attttaatct atccccgact agcacctttg tcttaaaatc aaggtttttt tctttaggca
      181 tacattgttt taattttttt aattcctttt ctaatttttt ccttttctca atattcgata
      241 taagttcatc aacttcatct gcttttacat atttttccat tcgcttccca ttctgagtga
      301 ttctatgata ataataaata ttaccatgta cattcttttt agaaacgccg ccagccggta
      361 aaacagctat ctcacgctca atttctgcca tacgagctat tatttcttca tgattcatat
      421 ataatcacct accatttgtt atacccatta tacccattta aaactagctg ttcaagggta
      481 taaaaaagag acgattaata aatatcgcct cttactagtt tttttgaata tatatgataa
      541 taccccatta atagtgctgc atttacaatc catgtcaatg gccaacctaa gaatacagta
      601 attaattccg aactaaattg tgttgccaca aataaataga cttgtcttaa aataacgaaa
      661 cataaaatca ttgaatacat tggtattttc gataaaccaa caccccttag ggcacctgca
      721 gtaatatggg ataaaggaag gaaaatatag ccaggcgcaa agacagaaat catagtcaca
      781 ccagctacaa tcacatcttt atcttgtgag aatagaccaa ctaagaattc tccatttgta
      841 taaattaaga aagctcctaa tgcgataatc acttcacaca tcaatagtgt tgtatataaa
      901 ccttttctta cacgcttata gttttgagct ccatagttct gtcccacaaa cgttgtgata
      961 gccatcgaga agctttgaat tggaagctgc aagaaaccat ccaacttcgt tgttgtacta
     1021 tatcctgcaa cagctgccga tccaaattta gaaatatacg attgtacgat tacattggaa
     1081 aaagaaacaa tactattttg tagggctgcc ggaattccaa tcgaaataat tcttgataga
     1141 attggctttg aaaaacgaat cttagaaata tataccttat actctttatc tgtatgcatc
     1201 aattcatgca tgactaaaat agcggataca agctgtgcaa gtgttgtcgc aatacccaca
     1261 ccggcaacac ctaaatgaaa gtagattaca aacgcaaagt ctaatataat attgactaaa
     1321 cttgatacaa ctaagtagat caatggtttt ttactatcac ctaaagctct taaaatcccg
     1381 gctcccatat tataaataag agaaaaggag acgcccgcaa aatagatttg aagataaatc
     1441 acagccaaag gtaaaacttc tttaggtgaa ccaatcataa ctaaaatggg tttagaaaga
     1501 gcaataccta atattgtgaa taagacacat aaaactaaag ttaaagctaa agatgtatga
     1561 atggaatcct gcatcttttt tgtttctttt gccccaaaat attgtgcaat cacaacacca
     1621 gcaccggttg ataatcccat aaagaatcca actaacatgt tgataatcga tgtagattgt
     1681 cctacagcag ctaaagcatg tgaactgaca aagtttccta ccacataaga atcgaccgtg
     1741 ttatataatt gttgaaataa atttccaacc agtaatggta aagaaaatat caatattgat
     1801 ttccatatac ttccctttaa taaatctgta ctctccaatg ccttcaccct tcccaaaaga
     1861 aaatgcagaa ttccatattg gtcttctaca taatgtaaaa taattttaac tcttttctga
     1921 aaaaaatgaa agcttatcct aaaattacat ccaataccat catcacacaa aatccaaaca
     1981 agaaagaaag ggttggtata ttcgaatgtt ttccttgtgc catttccgga accagttctt
     2041 caatcaccac aaacaacata gcgcctgccg caaaacttaa acaaagtggc aagcattgta
     2101 aaatccaatt tgaaaataag attgtgatat agccaaatat tggttctaca atacccgata
     2161 atgcaccaat caaaaacgcc ttatgttttg aatatccatt actctttaag ggtaaagata
     2221 aaatggcgcc ttcgggaaaa ttttgtatcg caatcccaat agataaagct aaagcggaat
     2281 acaaattttg tgtgtttaaa gcttgcgcca ataccacacc aacggccata ccttctggaa
     2341 tattatgcaa agtaaccgca aaaactagct ttgtcgtttt cttaagatgt gattcttttc
     2401 cctcttcatc atttgttcca ggatgcatat gaggaacaat acaatccaag gctaaaagta
     2461 aaagagcacc taagaatata cccagcacaa tgatcaaaat atttttttct tgttcgattg
     2521 ccggaatcaa taaggaccaa atacttgcgg ctatcatgac accagaggca atacctgaaa
     2581 aacatctaga taatagttca ttatttttat tttccataaa atacacaact gctgagccta
     2641 aagtagtacc aaaaaaagga attagtaatc ctaaccacat aatcatcacc atagtaagtt
     2701 taaactaatt cctttatatt cgtcaattca aacgcaaatt atatgcaaat ccatgatgtt
     2761 caatataatc ctctaagatc catcctttat catgaatttc tttagctgct tcttttccta
     2821 catatctaaa gtgccatggc tcattagtag cgcccgttct atctcctgca ttttctggga
     2881 aacgcaaaat gaatccatat ttataggcat tttcttcaac ccaatcatag tctggtgatt
     2941 ctccaaacgt tccatattct ccatccaata cactttgact tgttagatcc acactcaatc
     3001 cgcattggtg ttcacttgat cctggagtcg aaactaagct tgccgcataa gccgcatcat
     3061 acatcgcaaa atattcatta taaacagcta attgtgtttc ataagaacga tatgaactct
     3121 tgattgcaat actataacct tttttcaaac catccttata catattctca agcgcagttg
     3181 ccgcttcatc ccttaattgt ccaacttcac tttcatatgg aatattcaca tctcttaaat
     3241 cgcttggtac ataatcgctt ggtacactaa aaccattttt aattaaaacc aacatatttg
     3301 caggatcctc aattgtatat gtacgatcac cacgcttact tgaatcaata cctggataag
     3361 aaacggttaa tacagctttt aaaggcttta atccagaatc cacatattcc ttgatatctt
     3421 tgatttgtgc ccctttaatt gccaaatatt tctttgcctt tttataaggt acacttgctg
     3481 aaatcaatgt atccaaattt gaaatggtat atatatccga attattaaat aacacatccg
     3541 ttgtatagcc caaataattt aagtcttcca ttctttcata gtaagaatca atataataaa
     3601 caactttctt tacactctct ttagaagccc tgtaatactt gtcatacaat aaatagtgcg
     3661 aatcattttt tactttattc cacttcgaaa tatcaataat ttgatcatag tccaagatat
     3721 ctttgatatc atctgtttct aattttaata caactttctt atcctcttta tgatatcctt
     3781 taatactcaa tcgaatactt gtaaaattcg caatcaataa tgcgatacaa ataagcggaa
     3841 tcagcactac aattataatt ctttttctgt tcacttttct ttttactttt ttcataaatt
     3901 cacctaaatc attatataaa atgcaatgcg gaaagacaaa tttcaatttt ttacatttaa
     3961 tatgtaattt ttttcaaggc atgttacaat acctcgtaga ggtgattata tgttattcaa
     4021 aaacgcaacc atttatacaa tggaacaaga cccttttgta ggtgatttta gaatcgataa
     4081 gggtgtattt acagaagtag gaaaagattt aaatccaaaa gatgaagaag tacaagatct
     4141 aaatggtctt tttgtatttc ctggtttagt tgaaagtcat tgccatttag gtatggaaga
     4201 aacagccatt cgctttgaag gtgatgatgt caatgaaatc acagatccaa ttacaccaaa
     4261 catgcgtggt attgatggat gtaatccaat ggatgaaacc gtagaactcg cattaaaagg
     4321 tggtgttact acagttgctg ctggtcctgg aagtgctaac gttcttggtg gaacattctt
     4381 tgcgtataag acaaaaggaa attgtattga tgaaatgaca attcaaaatc caattgcgat
     4441 gaaggctgcc tttggtgaaa atcctaagcg ttgttatcaa ggaaaaagta ttgatacgcg
     4501 tatgcaaatc agtgcattgt taagagaaac attggaaaag acaaaggaat acatgaagaa
     4561 aaaggaatca ggtaaagatg tagcttatga tcaaaaatta gaagccatga ttccagtcat
     4621 caaaaaagaa cttccattaa agtgccacgc ccatagagcc gatgacattt taactgtcat
     4681 tcgtattgcg aaagaatatg acattgaagt cactttagat catgccactg atgcaagatg
     4741 tattgttgaa cagatcaaag aaagtggttt cccatgtatt tgtggaccta gctttggtca
     4801 taagacaaaa tttgaattga aatccaaatc ctttaaaaca ccaggcgtac taaataaagc
     4861 tggaatttta gtttccatca caaccgattc tccagttatt ccagaacaat atctatcttt
     4921 atgtgcagct ttagccgcaa aagaaggtat ggatgaacta gaagccatta aagccatcac
     4981 aatcaatcca gctaagatct taaaattaga tgatcgaatt ggttcgatta aatctggtaa
     5041 agatgctgac tttatcattt gtacaaagaa tattcttgat acgacaaatg aaatcaaagc
     5101 tgtttatgtc gatggaaaga aggctgtgta atgttattta aaaatgccac tatttatacc
     5161 atggaacaag acccattcgt tggtgatttt aaaatcgata aaggagtatt tactgaaatt
     5221 ggaaaagatt taagtccaaa agatgaagaa gtacaagatt taaatggact ttacgtattt
     5281 ccaggactta ttgacgccca cagccattta ggcatggtat gttcttccat tggttttgaa
     5341 ggtgaagatg gtaatgaagt cacagatcca gtcacaccaa acattcgtgg aattgatgga
     5401 tgtaatccaa tggatgaaac gattgaagaa gctttaaaat caggagttac aaccgtagct
     5461 gcaggacctg gtagcgcaaa cgtcattggc ggaactttct ttgcgtataa aactgcaggt
     5521 cattgtattg atgaaatgac aattcaaaat cctttagcta tgaaagctgc ctttggtgaa
     5581 aatcctaagc gttgttataa gggaaagaaa attgatacgc gtatgcaaat cagtgctcta
     5641 cttagagaaa ctcttgctaa gacaaaagaa tacattcaga aaaaagaatc tggtaaagat
     5701 gtagactatg atcaaaagct agaagccatg attcctgtca taaaaaaaca aatgccatta
     5761 aagtgtcatt gtcatagagc cgatgatatt ttaactgtga ttcgtattgc gaaagaatat
     5821 gatattgaag tgacattaga tcatgtaaca gatggaagaa gcattattcc acaaattaaa
     5881 gagagtggtt ttccatgtat tttaggacct tgcttaggac ataaatcaaa gtatgaagtt
     5941 aaaaatatgt cttttgaaac agccaatgaa ttctataaag ctggtattcc attctgcatt
     6001 atcacagact cccctgtcgt acccgaacaa tacttatcct taagtgcagc actagcacat
     6061 aaggcaggtc taccagaata tgaagccatt aaggccatca caatcaatcc agctaaaatt
     6121 ttaaaactag atgatcgtat tggatctatt aaagttggta aagatgcaga ctttgtcgtt
     6181 tgcacaaaaa atatattaga tacacaaaat gaaattaaga cagtatatgt caatggaaag
     6241 aaggaagcat aatgttattt aaaaacgcaa ccatttatac aatggaacaa gaaccttttg
     6301 taggcgattt taaaatcgat aaaggtgtat ttaccgaagt aggaaaaaat ttgacagcaa
     6361 gtgaaggaga agatgtacaa gatttaaatg gtctttacgt atttcctggt ttagttgaaa
     6421 gtcattgcca tttaggtatg gaagaaacag ccattcgctt tgaaggcgat gatgtcaatg
     6481 aaattacaga tccaatcaca ccaaatatgc gtggtataga tggatgtaat ccaatggatg
     6541 aaaccattga atccgcttta aaaggtggtg ttactacagt agctgcagga cctggtagtg
     6601 ctaacgtact tggtggaact ttctttgcgt ataagacaaa aggaaattgt attgatgaaa
     6661 tgacaattca aaatccaatt gcgatgaagg cagcctttgg tgaaaatcct aagcgttgtt
     6721 ataaagataa gaaaattgat acgcgtatgc agatcagtgc attattaaga gaaacattag
     6781 aaaagacaaa ggaatacatg aagaaaaaag aagacggaaa agatgtagct tatgatcaaa
     6841 aacttgaagc catgattcct gttgttaaaa gagaacttcc attaaagtgt catgctcata
     6901 gagccgatga tatcttaacg gccattcgta tcgcaaaaga agaaaatatc aagattacat
     6961 tagatcatgt aacggatgca agaagcattc ttcctcaaat caaagaaagt ggttttccat
     7021 gtatttgtgg acctgcattg actcacaaat caaaatttga acttgcgaat atgtcttttg
     7081 aaacaccaaa cgaactctat aaagcaggta tcttattctc tattattacc gactctccag
     7141 ttattcctca acaatatcta tctttaagtg ccgctctagc cactaaagca ggtcttccag
     7201 aatatgaagc cattaaagcc atcacaatca acccagctaa gattttaggt ttagataatc
     7261 gtgttggatc tattaaagtt ggtaaagacg ctgactttgt catttgtaca aagaacattc
     7321 tagacactca aaatgaaatc caatttgtct atgttgatgg aaagaaggcc gcttaatgct
     7381 ttttaaaaac gccactattt atacgatgga acaagatcca ttcgttggtg attttagagt
     7441 gaataaagga atatttacag agattggaaa aaatttagaa cccaaagatg aagatgttca
     7501 agatcttaca ggattcaatg tatatccagg catggttgaa gcccattgtc atttgggcat
     7561 ggaagaaacg gctattggtt ttgaaggcaa tgatttaaat gaaatcacaa atccaatcac
     7621 acccaacatg cgtggtattg atggatgtaa tccaatggat gaaactgtac aaactgcatt
     7681 agaagccggt atcacaacag tagctgcagg acctggtagt gccaatgtga tcggtggaac
     7741 tttctttgca tataagacaa aaggaaattg tattgatgaa atgaccattc aaaatccaat
     7801 tgcgatgaag gccgcattcg gcgaaaaccc aaagaattgc tacaaaggaa aaaatattga
     7861 tacacgtatg caaatcagcg cattactgag agaaactcta gctaagacaa aagaatacat
     7921 gatgaaaaaa gaaacaggta aagatgtaga ttatgatcaa aaacttgaag ctatgattcc
     7981 agtcatcaaa aaagaaatcc cattaaaatg tcatgctcat agagccgatg atatgttgac
     8041 cgccattcgt atcgcaaaag aatatgatat tgaaatcact ctagatcatg cgaccgattc
     8101 acaagcgatt ctagaccaaa tcaaagaaag tggttttcca tgtatttgtg gaccaagtct
     8161 ttgtcacaaa gataagtttg aatgtgccaa cgattcattt gaaacaccaa ataagatgta
     8221 tgaaaaagga atcttgtttg cgatcacaac cgattcccca ttcaatcccc aacaatatct
     8281 tccaatcaat gcagccctag cccataaagc aggactacct gaaaatgaag caatcaaggc
     8341 tatcacaatc aatccagcta aaatcttaca tttagagaat cgagttggtt ctattaaaac
     8401 tggtaaagat gctgacttta ttatttgtac taaaagtttg attgatttga ataatacaat
     8461 taaaaatgta tatattaatg gaataaaagc tgtctaagac agctttttat atgttaaaat
     8521 aatgtggttg gagaaaatag attatgaata tagttgaaaa atatggtgga accagcgttg
     8581 gaactattga acagattcaa gccattgcac ttcatgccaa acacatgaaa gaacaaggtc
     8641 acaatctagt cattgttgcc agtgccatgg gaaaaaccac aaataaatta atagaattag
     8701 ctcatagtgt cacaaacaat ccaaataaaa gagaattgga tagtttatta tctacgggtg
     8761 agcaacaaac aattacactt cttgcgattg cgatgaatgc gctcggtgtg gatgcgatca
     8821 gtttaactgg ctatcaagca ggttttctta ctagcaagca ccattctcgt gctttgatca
     8881 aagatattga tacaaccaca atccaatctc atttagacca aggaaaagtt gttgtcgtag
     8941 ccggctttca aggcgctact gaatatggag atattactac tttaggtcgt gggggaagtg
     9001 atacaacagc cgttgcgatt gccgcaaaac taaactatca atgccatatc tttacagatg
     9061 ttgcaggtgt ctacacaata gatccacgat tatatacgcg tgctaagaaa ctaaaatcaa
     9121 tttcttatga agaaatgatg cagatggcct gtttaggtgc tggagtatta gaaactcgaa
     9181 gtgttgaact tgctagtaaa tataatgttc cccttttctt aggaagagca ttggaaacag
     9241 atttagagaa aggaacttgg attatgcata aagaattaga agatatgcca attacaggtt
     9301 taagtgtaaa agacgactat gcgatcatgc gctttatgca cttacctaac gatggtgttt
     9361 atttaggaaa actatttaaa atgatttccg atttaaatat caaccttgat acaatttcac
     9421 aacaactaga tgatgaaggc ctagccaact ttactttata ttgttccgaa gaacaaagta
     9481 atcaaatcat ggaaaatgca tcaaaagaat atcctgtaca tcaaatgctt ggatatatca
     9541 aattgtcttt agtaggactt ggaatttcta cgcattcagg tattgcgagt aaagtattaa
     9601 acatcttaag tgaaaataat attaaatatt atatgatcac atcaagtgaa atctccattt
     9661 cattaacgat tgaaaagaaa gacaaagtta aagcggttca agctttagga aaggcctttg
     9721 atctataatg gaacttccag ttaaatttaa agaacaaatg aaaggacttc ttcaagatga
     9781 atatgaagac tatctacaaa gttttgatca tgaaagatat tatggattaa gaatcaatac
     9841 acttaagatt tctgttgaag aattcttaaa gatttctccc tttaaactaa aacccatccc
     9901 ttggacaaat gatggcttct attattcaag tgaagataaa cctagtaaat aaaatggtac
     9961 ccataaggag gacaacaacg tgacagttgt gatactcact tgtgggtatc ttttttcgtt
    10021 gttttaaaat aaatatgtca agagcc
//
LOCUS       DBMDWL010000070         9995 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2372_length_9995_cov_3.091874, whole genome shotgun sequence.
ACCESSION   DBMDWL010000070 DBMDWL010000000
VERSION     DBMDWL010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_9995_cov_3.091874"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="ACZPDN_06080"
     CDS             <1..985
                     /locus_tag="ACZPDN_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1298225.1"
                     /translation="GEAANIDFDEMNEELIRMGIVPKNLLAYKELSYAGYLKNGTLIT
                     DYQNEGMNAVNEIQEFYCLPLKDIQDYENIKGSLKPNEIYVYSNVEKVKEKTLSLFGK
                     EYVVKKQLDHLKMDENNPNVTEHYYIIVDSEKTLERMQEDQANVYGDNASAIRAYYSF
                     DCDANEREVIKKLGQNENINDFIWLSKLDQKQGLIELYAGFLFIGIFLSFLFIMATIL
                     IMYYKQITEGYEDKDRFEIMQKVGLEQQDVKKAIHSQVLTVFFMPLLVAGLHVCFAYP
                     MIEKLLHLLFVSDSWLYIRTTIFCFVAFALVYIVIYVLTSKIYYGIVKRNV"
     gene            1092..1802
                     /locus_tag="ACZPDN_06085"
     CDS             1092..1802
                     /locus_tag="ACZPDN_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1298226.1"
                     /translation="MFINGFDILWDEIDDYVADIQSIRSIDQFMFNKPVTFFCGENGS
                     GKSTLLEALAIKNGLNPEGGTRNYEFSTYDSYSSLHKHMRLWRSRKPDWMYFLRAESF
                     YNVATKEMEYGGIFSERYHDQSHGQSFFSVLMKQTNKNGLYFLDEPEAALSCQNQLAL
                     LYLIEECVKQNCQFIIATHSPILLGFPNAQILSFDDGKVEEVDYKSTSSYQTMKLFLD
                     RYDYFVKDIQENNEGGIL"
     gene            1799..2272
                     /locus_tag="ACZPDN_06090"
     CDS             1799..2272
                     /locus_tag="ACZPDN_06090"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0070038 - rRNA
                     (pseudouridine-N3-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1298227.1"
                     /translation="MIKLIAIGKNKDKALQSLEKEYMKRLSAFCKFSEIEVKDEANEH
                     VSREAEVLLVKEKEGNRVLEKLSDQDFVVLLDLHGKMIDSETFAKNLDTWQMKHSNIV
                     FVIAGSLGPSEKLVSRANYRWKLSDLTFTHLMTRVLMLEQIYRGFMINNGRNYHK"
     gene            2380..3564
                     /locus_tag="ACZPDN_06095"
     CDS             2380..3564
                     /locus_tag="ACZPDN_06095"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1298228.1"
                     /translation="MTKRTVKDLDVAGKKVIVRCDFNVPRKDGVITDDNRIVMALPTI
                     KYLVENKAKVILMSHLGKVKTEEDKAKNNLECVAVRLQELLPDVKVTFCPVTRGEELE
                     NAVNALADGEIVLMQNTRYEKGESKNDPELGAYWASLGEVFVEDAFGSVHRAHASTVG
                     IPTNIKENGLGFLVEKEVTMLGKAVDEPAHPFVAIIGGSKVSSKIDVVDNLLKKADKV
                     IIGGGMAYTFSKAVGGSIGTSLVEDDKLDLAKEYLEKAGDKLVLPVDNVIADAFSEDA
                     NTSVADAGQIPDGWMGLDIGPKSVELFRKTLAGAKTVVWNGPMGVFEMPKFAKGTEEV
                     CKAISELPEAITVIGGGDSAAAAKQLGYADKFSHVSTGGGASLEYMEGKTLPGIAIIS
                     EK"
     gene            3583..4329
                     /gene="tpiA"
                     /locus_tag="ACZPDN_06100"
     CDS             3583..4329
                     /gene="tpiA"
                     /locus_tag="ACZPDN_06100"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1298229.1"
                     /translation="MRKPIIVGNWKMNKNNAETKAFFEAVDGVAASENATYGIGCPFT
                     DLRTALDHAKNLVVAAENCHFEDSGAFTGEVSVPMLEEMGVKYCIIGHSERREMFGDT
                     DETVNKKAKRLIKAGITPILCVGETEADYDAGNSEKVIREELAGSLADLCPKCVGEMV
                     IAYEPIWAIGTGKSASVEIAENCCKIVRDQVKVMYGEEAAEKVRIQYGGSVKPNNIVE
                     YMAQPNIDGALIGGASLKEDSFTEIIEKTK"
     gene            4464..5543
                     /locus_tag="ACZPDN_06105"
     CDS             4464..5543
                     /locus_tag="ACZPDN_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HIR1298230.1"
                     /translation="MNFVENNGKKVAAILCVASAFPVVATVVAAKEEPGWHGDRYVNE
                     DKTVAKGWQEIEGKSYYFSEDGTVNSSKTEKATTASVSSNITGEVKETVATASQEAVK
                     QETEEKAVVEETPVQTEEVKTETTETTTDTTTDTTVTPDTAETPEAPAAPEQTPEQNP
                     TPAPEAPAAPEQTPEQDPTPAPEAPATPEQNPTPAPEAPTAPEQTPSEDTTTPAPTPD
                     QTVTPAPDQNTTPAPDQNTTPAPDQTASSLGDRISSSAQELVGVTDGLWCTQVVQQAL
                     ANAGVSDAYQLWPDQYQGVYGYYTDSPLAGDLIYYNNGGRGVDHIAVYIGNGQAVHGN
                     YNGKTVIADAYQSGMGNPQYIRVVR"
     gene            5631..6146
                     /locus_tag="ACZPDN_06110"
     CDS             5631..6146
                     /locus_tag="ACZPDN_06110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1298231.1"
                     /translation="MNEAYKNLLERRSVRKYNDKKVSHDLIEKIVYAGQFAPSGMNRQ
                     IYAFVVVEDEELMHELSKKNAEAMNSTSDPFYGAKSMIIVFADTNAPTYLYDGALAMG
                     NLMNAANALGVDSCWIHRAKEVFETDFGKELKKKWRLPESYEGIRHCILGYRDGELSK
                     RTERTSKVIYA"
     gene            6139..6624
                     /gene="ispF"
                     /locus_tag="ACZPDN_06115"
     CDS             6139..6624
                     /gene="ispF"
                     /locus_tag="ACZPDN_06115"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1298232.1"
                     /translation="MHNIRIGQAVDIHQLQEGRKLILGGVEIEHSKGLLGHSDADVLV
                     HAVGESILGALALGDLGKHFPDTDPKYKGANSLDLLAHIYEMMNEMGYQIGNVDATIL
                     AERPKMAPHIMAMRKNIAAVLHCDMEDISIKATRGEKLGFVGNEEGIVSLSVCILEKK
                     R"
     gene            6621..7070
                     /gene="dtd"
                     /locus_tag="ACZPDN_06120"
     CDS             6621..7070
                     /gene="dtd"
                     /locus_tag="ACZPDN_06120"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1298233.1"
                     /translation="MRVVIQRAKNAKCTVEGQVTGQIDYGYCLFVGFNNEDTEEIIDK
                     MIHKILFLRIFEDENGKMNKSIQDVNGSILSISQFTLYADCKKGNRPSFTNAGKPDLA
                     TVLYDQFNQKLQDQGMHVEKGIFGADMKIELLNDGPVTIVLDSKELF"
     gene            7070..7924
                     /gene="folD"
                     /locus_tag="ACZPDN_06125"
     CDS             7070..7924
                     /gene="folD"
                     /locus_tag="ACZPDN_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR1298234.1"
                     /translation="MEIIYGNVLADNIKSSMKQKIDDIRAEGARLPLLSVVLVGNNPA
                     SMSYVRGKEKACSSIGMLQKTIRLDENVSQEELSNVIMELNLDPEVDGILVQMPLPEH
                     LDETAALELIDPQKDVDGLHPMNAGCLLTNRPGFIPCTPQGVMAMLDSIGYKDLSGKR
                     AVVCGRSNLVGKPVALLLQNRNATVTIVHSKTPNIEQIASQADVLIVAMGVSQMVNEN
                     WVKEGAVVIDVGINRVNGKLVGDCDFESVAKKASYVSPVPKGVGPMTVCMLLSNTLDA
                     YKMHTQGE"
     gene            7927..8118
                     /locus_tag="ACZPDN_06130"
     CDS             7927..8118
                     /locus_tag="ACZPDN_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR1298235.1"
                     /translation="MEYGLNDIVEMKKEHPCHKSTEWKIIRMGADIRIKCLGCGASVL
                     MPRQKFEKKLKKVIRHEEE"
     gene            8137..9240
                     /gene="ychF"
                     /locus_tag="ACZPDN_06135"
     CDS             8137..9240
                     /gene="ychF"
                     /locus_tag="ACZPDN_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1298236.1"
                     /translation="MPLTAGIVGLPNVGKSTLFNAITKSQVEAANYPFATIQPNVGVV
                     EVPDYRIDRLVEIFNPKKTIYTTFEFTDIAGLVKGASQGEGLGNQFLSNIRLTDAICH
                     VVRCFDNPDITHVENSVDPIRDIEIINLELTLADLQTIENRRSKIERKAKTNKDKESL
                     DELALLDRLQPVLEEGKPARSLELNEEEQILMKGYNLLTAKPMIYVANLDDEGIMDPD
                     SCAYYNQVKEYAQSQNNQVVALCAQMEEELSGLDKEEKDAFLEELGVETSGLDQLIQE
                     AYSVLDLCTFLTAGEDECRAWTFKKGMKAPDCAGVIHTDFKKGFIRAEVYRFDDIDEL
                     GSELAIKEAGRLRLEGKDYVVQDGDVMHFRFNV"
     gene            9233..9874
                     /locus_tag="ACZPDN_06140"
     CDS             9233..9874
                     /locus_tag="ACZPDN_06140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR1298237.1"
                     /translation="MFSLQSIVYLVIAVILSMSIHETAHGLVSYWMGDPTAKLEGRLS
                     LNPFKHVDWLGLVCLLICGFGWAKPVPIDARYYKDAKTGIIWTSFAGPVANFLLSFIC
                     IFLFYALYKFAPQFMMTSVGYFVSNTLTYTGLISTGFGIFNLIPVPPLDGSKILFSFL
                     PDDKYYKFIDGAPWMNILFIFLIFTGLLDYPLGMLRSNMIQFFEMIAKMILGF"
BASE COUNT         3367 a         1381 c         2167 g         3080 t
ORIGIN      
        1 tggggaagct gccaatattg attttgatga aatgaatgaa gaattgattc gtatgggcat
       61 tgttccaaag aatcttcttg cgtataaaga attgagctat gctggatatt taaagaatgg
      121 aactttgatt acagattacc agaatgaggg gatgaatgcc gtaaatgaaa ttcaagaatt
      181 ttattgtctt ccactaaaag atattcaaga ttatgaaaat ataaagggtt ctttaaagcc
      241 gaatgaaatc tatgtgtatt ctaatgtaga gaaggttaag gagaagactt tatctttatt
      301 tggaaaagaa tatgttgtga agaaacaatt ggatcatttg aagatggatg aaaataatcc
      361 aaatgtgaca gagcattatt atattattgt ggattcagaa aagactttag agaggatgca
      421 agaagatcag gcaaatgtgt atggcgacaa cgcgagtgca atacgtgctt attattcgtt
      481 tgattgtgat gcgaatgagc gtgaagttat taaaaagtta ggtcagaatg agaatataaa
      541 tgattttatt tggttaagta aattggatca gaaacaagga ttgattgaat tatatgcagg
      601 cttcttattt attggaatct tcttaagctt cttatttatt atggcaacca ttctaattat
      661 gtactacaaa caaattacag aaggctatga agataaggat cgttttgaaa ttatgcaaaa
      721 agtaggtctt gaacaacaag atgtaaagaa ggctattcat tcacaggtat taacggtatt
      781 ctttatgcca ttacttgtgg cagggcttca tgtttgtttt gcgtatccaa tgattgaaaa
      841 gctacttcat ttattatttg tgagtgattc atggctttat attcgtacga caatattctg
      901 ttttgtggcc tttgcgctag tctacattgt gatttatgta ttaacttcaa aaatctatta
      961 tggaattgta aagagaaatg tttaattgga tattagggtt tctcttgatt gggttatgta
     1021 tctatgtata ttatgtgaat catcatggaa tttagtactc gaatagagta ctattttttt
     1081 aggtgatata tatgtttatt aatggatttg acatcttgtg ggatgaaatt gatgattatg
     1141 tggccgatat tcaaagtatt cgaagtatcg atcaattcat gtttaataag ccggttactt
     1201 tcttttgtgg cgaaaacgga agtggtaaat ccactttatt agaggctctt gcgattaaaa
     1261 atggattgaa tccagaaggt ggaacaagaa attatgagtt ttctacttat gattcgtatt
     1321 ctagtttgca taaacatatg cgtttgtggc gttctagaaa accggattgg atgtattttt
     1381 tgcgtgcaga aagcttctat aatgttgcga caaaggaaat ggaatatgga ggaatttttt
     1441 ctgaaagata ccatgatcaa tcgcatggtc agagtttctt ttctgtattg atgaaacaaa
     1501 caaataaaaa tggattatac tttctagatg aacccgaagc agctttatct tgtcagaatc
     1561 aattggcctt actctatttg attgaggaat gtgtgaaaca aaattgtcag tttatcattg
     1621 cgacacattc acctatatta ttgggctttc ccaatgcaca aatcttatcg tttgatgatg
     1681 gtaaggtaga agaagtggat tataaatcta cttctagcta tcaaacaatg aaattgtttt
     1741 tggatcggta tgattatttt gtgaaagata ttcaagagaa taatgaagga gggatattgt
     1801 gattaaatta attgcgattg gaaagaataa agataaggcg ttacagtctt tagagaaaga
     1861 atatatgaag cgcttgtcgg ctttttgtaa gtttagtgaa attgaagtca aggatgaagc
     1921 aaatgagcat gtttctagag aagccgaggt tttattggtg aaagaaaaag aggggaatcg
     1981 tgttctagaa aaattatcgg atcaagattt tgtggtatta cttgatcttc atggcaaaat
     2041 gattgattct gagacgtttg cgaaaaattt ggatacttgg caaatgaagc attcaaatat
     2101 tgtttttgtg attgcgggaa gtctagggcc aagtgaaaaa ttagtttcta gagcgaatta
     2161 tcgttggaaa ttatcggatc taacttttac acatttaatg acgcgagtct tgatgttgga
     2221 gcagatttat cgtggtttta tgatcaataa tggtcgtaat taccacaagt aagtgatgaa
     2281 agcgcttttt gtggaatatt ttgcttgtga aaaactagtc aatcaattta tgtagtgtta
     2341 taatggatgt gcaaaaaaat gacagtagga ggaatataca tgacaaagag aactgttaaa
     2401 gaccttgatg tagctggaaa aaaggtcatc gtacgttgtg attttaacgt gccaagaaaa
     2461 gatggtgtta tcactgatga taaccgtatc gtaatggctt tacctacaat taaatattta
     2521 gtagaaaaca aagctaaagt tattttgatg tctcacttag gaaaagttaa gactgaagaa
     2581 gacaaagcta aaaacaactt ggaatgcgta gctgtacgtt tacaagaatt gttaccagat
     2641 gtaaaagtaa ctttctgccc agtaactcgt ggtgaagaat tagaaaacgc tgtaaatgca
     2701 ttagctgatg gtgaaatcgt attaatgcaa aatactcgtt acgaaaaagg cgaaagcaaa
     2761 aatgatcctg aattaggtgc atattgggct agcttaggtg aagtattcgt tgaagatgca
     2821 ttcggatctg ttcaccgtgc tcacgcttct actgtaggta tccctacaaa tattaaagaa
     2881 aacggattag gattcttagt agaaaaagaa gttacaatgt taggaaaagc tgttgatgaa
     2941 ccagctcatc catttgtagc tatcattggt ggatctaaag tatctagtaa gatcgatgtt
     3001 gttgacaact tattgaaaaa agctgataaa gttatcatcg gtggtggaat ggcttataca
     3061 ttctctaaag ctgttggtgg ttcaattggt acttcattag ttgaagatga caaattagat
     3121 ttagcaaaag aatatttaga aaaagctgga gacaaattag ttcttcctgt agataacgta
     3181 atcgctgatg cattcagtga agatgctaac acttctgttg cagatgctgg acaaattcct
     3241 gatggatgga tgggattaga tattggtcct aaatctgttg aattgttcag aaaaacttta
     3301 gctggtgcta aaactgtagt atggaatgga cctatgggtg tattcgaaat gcctaaattc
     3361 gctaaaggta ctgaagaagt atgtaaagca atctctgaat taccagaagc tattacagta
     3421 attggtggtg gagattctgc tgctgctgca aaacaattag gatatgcaga caaattctct
     3481 cacgtttcaa ctggtggtgg agctagcttg gaatatatgg aaggtaaaac attacctggt
     3541 atcgctatta tcagcgaaaa gtaataagga gatgtactta agatgagaaa acctattatc
     3601 gttggaaact ggaaaatgaa taaaaacaac gctgaaacaa aagctttctt cgaagctgtt
     3661 gatggtgttg ctgctagcga aaatgctact tatggaattg gatgtccatt tactgattta
     3721 agaactgcat tggatcacgc taaaaactta gttgtagctg ctgaaaactg ccactttgaa
     3781 gatagtggag ctttcactgg tgaagttagt gttcctatgt tagaagaaat gggagttaaa
     3841 tattgcatta tcggtcactc agaacgtcgt gaaatgttcg gtgatacaga tgaaacagta
     3901 aacaaaaaag cgaaacgttt gatcaaagct ggaatcactc caatcttatg tgttggtgaa
     3961 acagaagctg attacgatgc tggaaactca gaaaaagtaa ttcgtgaaga attagctggt
     4021 tctttagctg acttatgtcc aaaatgtgtt ggtgaaatgg taattgctta cgaaccaatc
     4081 tgggctatcg gaactggaaa atctgcttca gttgaaattg ctgaaaactg ctgtaagatc
     4141 gtacgtgacc aagttaaggt tatgtatggt gaagaagctg ctgaaaaagt acgtattcaa
     4201 tatggtggaa gtgttaaacc taacaatatc gttgaatata tggctcaacc aaatatcgat
     4261 ggtgcattaa ttggtggtgc tagcttgaaa gaagatagct tcacagaaat cattgaaaaa
     4321 acaaaataat tgatgatcat ataggcctct ttgaggcctt ttatttttag ctgtttctaa
     4381 ccaaaaataa attttagggt tgtttggaag gtggtttcgt ggtaaactaa tcatgaacta
     4441 ttttaaatag ggggtagaat aaaatgaact ttgtagaaaa taatggtaaa aaagtcgcag
     4501 caattctttg tgtagcatct gctttccctg ttgtcgcaac agtggttgca gccaaggaag
     4561 agccaggatg gcatggcgat cgttacgtaa atgaagataa aactgtagct aaaggatggc
     4621 aagaaatcga aggaaaatct tactatttct cagaagatgg tacagtaaac tcttcaaaaa
     4681 ctgaaaaagc cactactgct agcgtttcaa gtaacattac aggggaagtg aaagaaacag
     4741 tagcgacagc tagtcaagaa gctgtaaaac aagaaacaga agaaaaagca gttgtagaag
     4801 aaacacctgt tcaaactgaa gaagttaaaa cagaaacaac agaaacaact actgatacaa
     4861 caacggatac tactgtaact ccggatactg ctgaaactcc agaagctcct gcagctccag
     4921 aacagactcc agagcaaaat ccaacacctg ctccagaagc tcctgcagct ccagaacaga
     4981 ctccagagca agatccaaca cctgctccag aagctcctgc aactccagaa caaaatccaa
     5041 cacctgctcc agaagctcca acagctccag aacagacacc aagtgaggat acaacaactc
     5101 cagctccaac tccagatcag acagttactc ctgctccaga tcagaataca acacctgctc
     5161 cggatcagaa cacaacacct gctccagatc aaacagcttc tagcttaggt gatcgtattt
     5221 cttcatctgc tcaagaatta gtcggtgtaa cggatggatt atggtgtaca caagttgtgc
     5281 aacaagcatt agcaaatgcg ggtgtaagtg atgcatatca attatggcca gatcaatatc
     5341 aaggtgtata tggatattac acagatagtc ctttagctgg tgacttaatt tactacaaca
     5401 atggtggacg tggtgttgac cacatcgctg tatatattgg aaatggacaa gctgtacacg
     5461 gaaactacaa tggtaaaact gtaatagctg acgcatatca aagtggtatg ggtaatccac
     5521 aatatattcg tgtagtaaga taattgaatt tttaagcttg gcaatttgcc aggctttttt
     5581 ttaattcttt tgtattgtat aatatgatgt agttttggag gctattattt atgaatgaag
     5641 cttataaaaa tcttctagaa agaagaagtg ttagaaaata taatgataaa aaggtcagtc
     5701 atgacttaat tgaaaaaatt gtatatgcag gtcaatttgc gcctagtggc atgaatcgtc
     5761 aaatttatgc ctttgtggtt gtagaagatg aagaattgat gcatgaacta tcgaaaaaga
     5821 atgcagaagc tatgaattct acatcggatc ctttctatgg ggctaaaagt atgattattg
     5881 tatttgcgga tacaaatgca ccgacatatt tatatgatgg agccttagct atgggaaatc
     5941 taatgaatgc agctaatgct ttaggcgtcg attcatgttg gatccatcga gctaaagaag
     6001 tttttgaaac ggactttggc aaagaattaa agaaaaaatg gcgcttacca gaatcttatg
     6061 aaggtattag acattgtatt ttaggctata gagatggaga actttcaaag cgcactgaaa
     6121 gaacttctaa ggtgatttat gcataatata cgtattggac aagcggtaga tattcatcaa
     6181 cttcaagagg gtagaaaact cattctaggc ggtgtagaaa tcgaacattc aaaaggtttg
     6241 ttaggacatt cagatgccga tgttttagta catgcggtag gtgaaagtat tttaggagcc
     6301 cttgcattag gagatctagg aaagcatttt ccggatacgg atcctaaata taaaggagcg
     6361 aatagtttag atttattggc tcatatctat gaaatgatga atgaaatggg ttaccagatt
     6421 ggaaatgtgg atgcgaccat cttggcagaa agaccgaaaa tggcaccaca tattatggct
     6481 atgcgaaaaa atattgcagc tgttttacat tgtgatatgg aagatatttc catcaaggca
     6541 acgcgtggag aaaagctagg ctttgtagga aatgaagagg gcatagtgag tttaagtgtt
     6601 tgtatattgg agaagaaaag atgagagttg taattcaaag ggcaaaaaac gcaaaatgta
     6661 cagttgaagg gcaagtgaca ggacaaattg attatggcta ttgtcttttt gtaggtttta
     6721 ataatgagga tacagaagaa atcattgata aaatgattca taaaatttta tttttacgta
     6781 tttttgaaga tgaaaatggg aagatgaata aatcgattca agatgtaaat ggtagtattc
     6841 tatctatttc acaatttact ttatatgccg attgtaaaaa gggaaatcgc ccaagtttta
     6901 caaatgcagg aaagccagat ttagctacag tattatatga tcaatttaat cagaaattac
     6961 aagatcaagg tatgcatgta gaaaagggga tttttggagc cgatatgaag attgaattgt
     7021 tgaatgatgg tccagtcacg attgtattgg attcgaagga gttgttttaa tggaaattat
     7081 atatggaaat gtacttgcgg ataacataaa gtcttcaatg aaacaaaaga ttgatgacat
     7141 tcgtgctgaa ggagctagat tgcctttact aagtgtagta cttgtgggaa ataatccagc
     7201 aagtatgtct tatgttcgtg gtaaagaaaa ggcttgttct tcaataggaa tgcttcagaa
     7261 aacgattcgc ttagatgaaa atgtatcgca agaagaattg agtaatgtga ttatggagct
     7321 aaatctagat ccggaagtgg atggtatttt agtccaaatg ccattgccag aacatttaga
     7381 tgaaacggcc gcattagaat tgattgatcc acaaaaagat gtggatggtt tacaccccat
     7441 gaatgccggg tgtttattga caaatcgtcc aggctttatt ccatgtactc cacaaggtgt
     7501 gatggctatg ttagattcga ttggatataa agatctatct ggaaaacgtg cggttgtttg
     7561 tggaagaagt aatttagttg gaaaacctgt cgcattattg cttcaaaacc gcaatgcgac
     7621 agttacaatt gtacattcga aaacaccaaa tattgaacag attgcttcac aagcggatgt
     7681 tttgattgtt gcgatgggtg ttagtcaaat ggtcaatgaa aattgggtaa aagaaggagc
     7741 tgtcgtgatt gatgtgggaa tcaatcgtgt caatggaaaa cttgttgggg attgtgactt
     7801 cgaatcggtt gcgaaaaagg caagttatgt aagtcctgtg ccaaaaggtg ttggtccaat
     7861 gacggtttgt atgttattat cgaatacgct agatgcatat aaaatgcata cgcaaggaga
     7921 ataattatgg aatatggatt aaatgatatt gttgaaatga aaaaagagca tccttgtcat
     7981 aagtccacag aatggaaaat tattcgcatg ggtgctgata ttcgaattaa gtgtttgggt
     8041 tgtggagcaa gtgttttgat gcctagacaa aaatttgaaa agaaattgaa aaaagtaatt
     8101 cgacatgaag aagaatagag aggatgtatt gaatttatgc cattaactgc aggaattgtt
     8161 ggtcttccta acgtaggaaa atcgacttta tttaacgcta tcacaaaatc tcaagtggaa
     8221 gctgcaaact atccatttgc gacaattcag ccaaatgtag gtgttgtaga agtaccagac
     8281 tatcgtattg atcgtttagt tgaaatcttc aatccgaaaa aaacgattta tacaacattt
     8341 gaatttactg atattgcagg attagtaaag ggagcaagtc aaggtgaagg tttgggtaac
     8401 caattcttat cgaacattcg tttaacagat gcgatttgtc acgtagtgcg ttgctttgac
     8461 aatccggata ttacacacgt agaaaatagt gtggatccaa ttcgtgatat tgaaattatc
     8521 aacttagaat tgactttggc tgaccttcaa acgattgaaa atcgtagaag taagattgaa
     8581 cgtaaggcta agacaaacaa agataaagaa tcattggatg aacttgcttt attggatcgt
     8641 cttcaacctg ttttagaaga aggaaaacca gcacgttcat tagaattaaa tgaagaagaa
     8701 caaattttga tgaagggtta taacttattg accgcaaaac ctatgattta tgttgccaac
     8761 ttagatgatg aagggattat ggatccagat tcatgtgctt actacaatca agtaaaggaa
     8821 tatgcacaaa gtcaaaataa ccaagtcgtt gccttatgtg cgcaaatgga agaggaattg
     8881 tctggtttag ataaagaaga aaaagatgcg ttcttagaag aattaggtgt tgaaacttct
     8941 ggtttagatc aattgattca agaagcctat tccgtattag atttatgtac attcttaacg
     9001 gctggagaag atgaatgtcg tgcatggaca ttcaaaaaag gtatgaaagc accggattgt
     9061 gctggtgtta tccacacaga tttcaaaaaa ggctttattc gagcagaagt atatcgcttt
     9121 gatgatattg atgaattggg tagtgaatta gctattaaag aagcaggacg tttacgtcta
     9181 gaaggaaagg attatgtcgt tcaagatggc gatgtaatgc attttagatt taatgtttag
     9241 tctacaaagt attgtctatt tggtgattgc cgttattcta tctatgtcaa ttcatgaaac
     9301 agctcatggc ttagtttctt attggatggg agatccaacc gctaaattag agggaagatt
     9361 aagtctaaat ccttttaaac atgtggattg gctaggctta gtttgtctat tgatttgtgg
     9421 atttggatgg gcaaaacccg ttccgattga tgcaagatat tataaagacg caaaaacagg
     9481 gattatttgg acaagttttg cggggcctgt cgcaaatttt cttttaagct ttatttgtat
     9541 attcctattt tatgcacttt ataaatttgc acctcaattt atgatgacaa gtgttggtta
     9601 ttttgtatca aacacattga cctatacagg acttatttct acgggatttg gaatttttaa
     9661 cttaattcca gttccaccac tagatggatc gaaaatatta ttttcatttt taccagatga
     9721 taagtattat aaatttattg atggtgcacc atggatgaac atcttattta tctttttgat
     9781 ttttacaggt ctgttagatt atccactagg tatgttacgt tcaaatatga ttcaattttt
     9841 tgagatgatc gcaaagatga ttttaggttt ctaaagtcat cttttttaaa cgctttcaca
     9901 aaaaaatgac attttactaa aaattaacat aaatttaata ttgttttgac attattttta
     9961 ttgcataata aagacgtcgc aatgtggaat ttttt
//
LOCUS       DBMDWL010000071         9788 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2457_length_9788_cov_2.163105, whole genome shotgun sequence.
ACCESSION   DBMDWL010000071 DBMDWL010000000
VERSION     DBMDWL010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9788
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2457_length_9788_cov_2.163105"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="ACZPDN_06145"
     CDS             complement(<1..330)
                     /locus_tag="ACZPDN_06145"
                     /inference="COORDINATES: protein motif:HMM:NF037631.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HIR1298238.1"
                     /translation="MVGHEVFISQMKDQIRIFQKQNRRIMTYFLSISEQAILQNMVPK
                     NICVVFDGGYENAERKIACFIPEGYEGFSDCVCLHATYNSKFKTLTHSDLLGCLMHSG
                     IERSVIGD"
     gene            complement(355..786)
                     /locus_tag="ACZPDN_06150"
     CDS             complement(355..786)
                     /locus_tag="ACZPDN_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR1298239.1"
                     /translation="MGFMDKVKDFVAPIEEEDDYQEAPQAEKVEEVEPQSRYERPKAR
                     TNATSLNSNTKMVLFEPRSFGEAEEVGQRLKEGRAVVVNLHKLDRDYAQRTIDFLTGV
                     VYALDGKIQKIGQNVILCSPAEIGVQGTISLGSPDDFEDED"
     gene            complement(903..1436)
                     /gene="hpf"
                     /locus_tag="ACZPDN_06155"
     CDS             complement(903..1436)
                     /gene="hpf"
                     /locus_tag="ACZPDN_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR1298240.1"
                     /translation="MKVNIVGKNITVTEGISDKIHKKLDVLTKYFIISEDDVANVLVR
                     TYPTKQKIEVTIPTRYAILRAEVVDDDLYAAIDLAVDKLEDQIRRTKTRLTRKNKESL
                     AEAFLDHELFNDEEVDDDEVVRTKSIVPKEMSLDEAIMKMELLGHDFFIYTDDETNKI
                     AVCYKRHDGGYGLIETE"
     gene            1785..2798
                     /gene="aroF"
                     /locus_tag="ACZPDN_06160"
     CDS             1785..2798
                     /gene="aroF"
                     /locus_tag="ACZPDN_06160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1298241.1"
                     /translation="MIITLKKNAPKHDVDKLIHKFEGMNLQVTMIQGDNYNVFGLVGD
                     TSIVDEKSVMANPIVYNVQRVAQPYKLANRMFHPEDTIVDVNGIKIGGKKIAMIAGPC
                     SVEGEDQICRIAKEVKQAGADMLRGGAYKPRTSPYAFQGMGTAGIMAMVKARQETKLP
                     IVSELMSAEHLDEFVENVDIIQIGARNMQNFDLLKAVGKTKKPILLKRGMSATIQEWI
                     MAAEYIMSEGNPNVIFCERGIRTFETYTRNTLDLSVVPIIKERTHLPIIIDPSHAAGD
                     YKLIKSLSLAAIAAGADGLIIEVHDDPEYAWSDGAQSLKPKRYAELVKKGKAIAKVVG
                     REL"
     gene            2849..3778
                     /gene="mnmL"
                     /locus_tag="ACZPDN_06165"
                     /gene_synonym="ytqA"
     CDS             2849..3778
                     /gene="mnmL"
                     /locus_tag="ACZPDN_06165"
                     /gene_synonym="ytqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864978.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification radical SAM protein MnmL/YtqA"
                     /protein_id="HIR1298242.1"
                     /translation="MNNPYPYSNDNKRYQTINYYFRNTYHQKVAKIPLNAGFTCPNRD
                     GTKGSGGCTFCSSMGSGDSILCFDDSLQKQYKEGLKRMQNKWPDCIGFAYFQSYSNTY
                     APLEALKKVYDPFFKNPDICGVAIATRADCLNDEIIDYLNSQNKEVWLELGLQSVHED
                     TMEKCNRKHSTQIVFDWLDKLKSTNIKTCVHIINGLPNESKSDMLETVKQISKHPIDA
                     IKIHMLHLIEGTQMAKEYKEHPFHILTLEEYVDIVVDQLELLPQEVIIGRLTGDGIAK
                     DLIEPKWTIKKTIVTNEIDKLMVKRNTWQGKNV"
     gene            complement(3795..5027)
                     /gene="tyrS"
                     /locus_tag="ACZPDN_06170"
     CDS             complement(3795..5027)
                     /gene="tyrS"
                     /locus_tag="ACZPDN_06170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1298243.1"
                     /translation="MELFEELKWRGLVDNVTSEEVEDVINNGEVTFYIGTDPTADSLH
                     IGHYSSLLMAKRLEKHGHHPIMLVGGATGFIGDPKATGERSMLTSEVLKSNYDALHAQ
                     ISKLWGFDMVNNLDWTKGITIIDFLRDYGKLFNVNYMLNKETVKKRLDSGISYTEFSY
                     MILQSLDFLNLYQTRGCTMQIGGQDQWGNITSGLELIRKKCGADVKCYGLTMPLITKA
                     DGTKFGKSETGTVWLDPKKTSPYELYQFLFNTDDSKVIEYLKKLTFLTKEEIDALEVS
                     LKEDAGRREAQKKLAEEVVRDLHGEEGLESALKITNALFRGQLNDLDEQQRLDAVKNM
                     DKVDQEAGKSLIDVLVEAKIASSRREAREWIKGNSISLNGVKVNDETLVIDSTQAYVS
                     NYVIIRRGKKRYYCLNLK"
     misc_binding    complement(5060..5255)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5281..6534)
                     /locus_tag="ACZPDN_06175"
     CDS             complement(5281..6534)
                     /locus_tag="ACZPDN_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HIR1298244.1"
                     /translation="MRIDTINIQQYSEYLDTVDTAVFQQSPFHAKKFEHDGWTVEFIQ
                     ASQEKEVYATCMLAYLPLMKVFKYCYVARGFLADYKDRDKLVKFTSSLKQYLKKKNVV
                     YLEIDPEIDLVQRDKDGNVVENGFHNYDVVDNLKSAGFLQLPLKQGYDLSKECRFCSS
                     IDLRGKTSDEIFNAFSSATRRNTRTAQKYNVHIRKLNVDQLHILDSMEKETSARQDFE
                     AMSLDYFKNLYRFYGEDHVETLLSYLDLDEYEQKIKKEYNTTFTSIEKTKVFLEKNPG
                     NEKKEKRLKTDETYLDSLEKKISHIQELKDTYGKEVPLACCLFIKYGHEIVYLVGSSN
                     YEQRDFRGPYAIHWHMIQEAIKEGYDFYNFYGISGYFEPGKEGYGVFDFKRGFNAIVH
                     EYIGNFILPCKPFIFKIYNNLKHIC"
     gene            complement(6546..8960)
                     /gene="leuS"
                     /locus_tag="ACZPDN_06180"
     CDS             complement(6546..8960)
                     /gene="leuS"
                     /locus_tag="ACZPDN_06180"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1298245.1"
                     /translation="MYDHLEVEKKWHKYWDEHETFKTDVWDFSKPKFYALDMFPYPSG
                     VGLHAGHPEGYTATDIVSRMKRMQGYNVLHPMGYDSFGLPAEQYAVQTGNNPNGFTQE
                     NIKTFTNQLKELGFDYDWSKMIATSDPDFYHWTQWIFKQLYKDGYAKYVDMPVNWCEE
                     LGTVLSNDEVIDGKSERGGYPVIRKNMKQLCIDQAAFAERLLEGLNEIDWPESTKEMQ
                     RNWIGRSTGVEVQFEIVGGGEFSIFTTCIETIYGITFMVLAPDGDLVQELMPRIKNQD
                     EVKAYIQETVSKSEMDRTELNKGKSGCRLEGIKAINPVNGREVEVFIGDFVLANYGTG
                     AVMAVPSHDQRDFEYAIAHNIDMIQVIEGQDVSEHAFEKQDYLGKGCKLINSEEFTGL
                     TVEEAKVAITKKLVDMGIAKETVNYRFREWIFARQRYWGEPVPIVHLEDGTDYVLPDD
                     ELPLILPELEDYKGHNGKAPLENATDWKQYNQNGVKGVRETSTMPGSAGSSWYYLRYI
                     DPQNKEQLGNPELLKHWMPVDLYVGGPEHAVGHLMYSRIWNNYLYDKGIVACKEPFKK
                     LVHQGMILGENGIKMGKRYPKFVVNPSDIVKKYGADTLRLYEMFMGPLEQSKPWSMAG
                     CDGARRWIDRVYRLFIESGKVIDTNDGKLDKVYNQTVKKVTEDYESLGFNTAISQMMI
                     FVNDAYKAETIYKPYAEGIVKMLSCITPHIGEEMWQLLGHEDTIAYEPWPTYDEAKLV
                     EDTITCVVQVNGKVRGKFMAAVGSSNEELEKQAMELETVKRQIEGKTIRKVIVVKGKV
                     VNIVAN"
     misc_binding    complement(8999..9196)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9215..9583)
                     /locus_tag="ACZPDN_06185"
     CDS             complement(9215..9583)
                     /locus_tag="ACZPDN_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298246.1"
                     /translation="MTLKSINGYASWTSLVCLFLVLQIVSFLTLSTIQNVYLLKANRQ
                     NILELSIVDHAKSMIYRNNRIKLCHTKEELIKEKDETIMNTRVHFQDYSTYMECTYDN
                     VCMKIYYDDKSIVDVVIDEP"
     gene            complement(9564..>9788)
                     /locus_tag="ACZPDN_06190"
     CDS             complement(9564..>9788)
                     /locus_tag="ACZPDN_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298247.1"
                     /translation="ELAQSNDLKIESDCLSYILNHEKKVLYFDKHRIVKSPGYEIYME
                     QIDLGYFYKKEDGYYLWFKKKNKIYDFEIY"
BASE COUNT         3132 a         1923 c         1406 g         3327 t
ORIGIN      
        1 atctccaatg acacttcttt caatacccga atgcattaaa caacctaata aatcagaatg
       61 tgtcagtgtt ttgaattttg aattgtatgt ggcatgaaga cagacacaat cactaaaacc
      121 ttcatagcct tccggtataa aacatgcgat ctttctttct gcattctcgt acccaccatc
      181 aaaaacgaca catatatttt tagggaccat gttttgaaga atggcttgtt cagaaatcga
      241 taaaaaatag gtcatgatcc gcctattttg tttttgaaag atacgaattt gatctttcat
      301 ctgcgaaata aacacctcat gacctaccat aaatgagctc attaaaagta attattagtc
      361 ttcatcttcg aagtcatcag gacttcctaa tgaaatagtt ccttgtacac caatttcagc
      421 tggagaacat aaaattacgt tctgaccaat tttctgaatt ttaccatcaa gtgcataaac
      481 aacacctgtt aaaaaatcaa tagtacgttg tgcatagtca cgatctagct tatgtaagtt
      541 aactacaaca gcacgacctt cttttaaacg ttgtcctact tcttcagctt ctccaaagct
      601 tcttggttca aacaatacca tttttgtatt cgaattcaaa gaagttgcgt tcgtacgagc
      661 ttttggtctc tcgtaacgac tttgaggttc tacttcttct accttttcag cttgtggtgc
      721 ttcttgataa tcgtcttctt cttcgattgg agcaacgaag tcttttactt tatccataaa
      781 tcccatggga aaaacctcct taacgaacat aattatagca caaagaataa cttattcaaa
      841 cttatattct tttttttatt tccaattaaa agacatgcca atttgacatg cctttttaca
      901 tattattcag tttcaattaa tccgtatcca ccatcatgac gtttgtagca aacggcaatt
      961 ttgtttgttt catcatctgt ataaatgaag aaatcatgtc ctaataattc catcttcata
     1021 atagcttcat ccaagctcat ttcttttgga acgattgatt tagtacgaac aacttcatca
     1081 tcatctactt cttcatcatt aaataattca tgatctaaga aagcttcggc aagtgattct
     1141 ttatttttac gtgttaaacg tgtttttgtt ctacgaattt gatcttccaa tttatctaca
     1201 gcaagatcga tagctgcata taaatcatcg tctacaacct ccgcacgaag aatcgcataa
     1261 cgcgttggga ttgtaacttc aattttttgt tttgtaggat atgtacgaac taaaacgtta
     1321 gctacatcat cctcactaat aatgaagtac tttgttaaaa catctaattt tttatggatc
     1381 ttatctgaga ttccttcagt gactgtgatg ttttttccta caatattgac tttcatatta
     1441 tgtacctcct atatgatttc tattgctact tagattataa cacttttatt ttgttttaca
     1501 cttttcgaca agaaatgaca aaaatataac caactttgcc aaggaggttg acaagcaaaa
     1561 tgtaactatt ttcagaaaat aacttgcaca ttttcaaaga ttaagctata gtatacacag
     1621 gtgattaaca tgaacattca atctacatat ttttactttt actttagata gccctaatag
     1681 aaccaatcaa aacagaatgg tatttttatt agggctttta agcattaaca attcgaacgg
     1741 ttaatgcttt tttgttactc acaaatggaa agaaggatat tactatgatt attactttaa
     1801 agaaaaatgc accaaaacac gatgtcgaca aattaattca caaattcgaa ggaatgaatc
     1861 ttcaagtcac aatgatccaa ggcgataact acaatgtctt tggtttagtt ggagatacaa
     1921 gtatcgtcga tgaaaaaagc gttatggcaa accccattgt atataacgta caacgtgttg
     1981 cccagcctta taaattagcc aatcgtatgt tccatccaga agataccatt gtagatgtaa
     2041 atggaattaa aatcggtggt aaaaaaatcg ccatgattgc aggcccatgt tccgtagagg
     2101 gtgaagatca aatatgtcgt attgccaaag aagttaaaca agcaggagcc gatatgttaa
     2161 gaggtggcgc ttataaacca agaacaagtc catacgcttt ccaaggtatg ggtacagctg
     2221 gaatcatggc catggtaaaa gctagacaag aaacaaaact tcctattgta tctgaactta
     2281 tgagtgcaga acatttggat gaattcgttg aaaatgtaga tatcattcag attggtgctc
     2341 gtaatatgca aaactttgac ttattaaaag ctgtaggaaa aacaaaaaaa ccaatcttat
     2401 taaaacgcgg tatgagtgct acgattcaag aatggatcat ggcagctgaa tatattatgt
     2461 ccgaaggaaa tccaaatgtc atcttctgtg aacgtggtat tcgtactttt gaaacctata
     2521 cacgtaatac attagattta agtgttgtac ccattattaa agaaagaaca cacttaccta
     2581 ttatcattga tccaagtcat gcagctggag attataaatt aattaaatct ttatctttag
     2641 ccgctattgc agctggagca gatggattga tcattgaagt gcatgatgat ccagaatatg
     2701 catggagtga tggagcccaa agcttaaaac ctaagcgcta tgccgaactt gttaaaaaag
     2761 gtaaagccat tgccaaagta gttggtagag aactataaga aactgcacac gcagtttctt
     2821 tttatgttac aatgctttgg gtgatacaat gaataatcct tatccttatt caaatgacaa
     2881 taaaagatac caaacaatca attactattt tcgtaataca taccatcaaa aagtggctaa
     2941 gattccattg aatgcaggtt ttacctgtcc aaatcgcgat ggaacaaaag gaagcggtgg
     3001 atgtactttc tgttccagta tgggctcagg cgatagtatt ctatgttttg atgattcgtt
     3061 gcagaaacaa tataaagaag gcttaaagcg catgcaaaat aagtggcctg attgtattgg
     3121 gtttgcctac tttcaaagct attccaatac atatgcccca cttgaagcct taaaaaaagt
     3181 atatgatcca ttctttaaaa atccagatat ttgtggagtt gcgattgcga ctcgtgccga
     3241 ttgtttaaat gatgaaatta tagactattt aaatagtcaa aataaagaag tctggctaga
     3301 actcggcctt caaagtgtac atgaagacac aatggaaaag tgtaatcgta aacatagtac
     3361 acaaattgtg tttgattggt tagacaaatt aaaatcgaca aacatcaaaa catgtgtaca
     3421 tattataaat ggtttaccaa atgaatccaa atccgacatg ttagaaacgg ttaaacagat
     3481 ttctaaacat cctatagatg cgattaagat ccatatgctg catttaatcg agggcacaca
     3541 aatggcaaaa gaatataaag aacatccatt tcatattcta actttagaag agtatgtcga
     3601 tattgtagtg gatcaattgg aattattacc acaagaagtt atcattggac gattaacagg
     3661 ggatggtatt gcgaaagatt tgattgaacc aaaatggaca atcaaaaaaa cgattgtgac
     3721 aaatgaaatt gataagttaa tggtaaaaag aaatacgtgg caaggcaaaa atgtctaacc
     3781 acgtattttt atttttattt caaatttaaa caataatatc ttttcttacc acgacgaatg
     3841 attacatagt ttgatacata ggcttgcgta gaatcaataa ctaatgtttc atcattgact
     3901 ttgacaccat ttaagctaat ggaatttcct ttgatccatt ctctagcttc acgacgactc
     3961 gaagcaatct tagcctccac taagacatca attaaagact ttccagcttc ttgatcaact
     4021 ttatccatat ttttaaccgc atccaaacgt tgttgttcat ccaaatcatt taattgacct
     4081 ctaaataagg catttgtgat ttttaaagct gattctaaac cttcttcacc atgtaaatca
     4141 cgaaccactt cttctgccaa tttcttttga gcttcacgtc ttccagcatc ttcctttaaa
     4201 gatacttcta aagcatcaat ttcttctttt gttaagaatg ttaatttctt taaatattca
     4261 ataacctttg aatcatctgt attaaacaag aattgatata gttcataagg agatgtcttt
     4321 ttaggatcca accaaacagt tcccgtttca ctcttaccaa atttagttcc atctgccttt
     4381 gtgatgagtg gcattgttaa gccataacat tttacatcgg caccacattt tttacgaatc
     4441 aattccaaac cagaagtgat atttccccat tgatcctgtc caccaatctg cattgtacaa
     4501 cctcttgttt ggtaaagatt taagaagtct aacgattgaa ggatcatata tgaaaattct
     4561 gtataactga ttcctgaatc caaacgtttt tttactgttt ctttattcag catataattc
     4621 acattgaata attttccata atcacgtaag aaatcaatga ttgtgatgcc ttttgtccaa
     4681 tctaagttat ttaccatgtc aaagccccat aatttagaga tttgtgcatg taatgcatca
     4741 taatttgact ttaatacttc actagtcaac atacttcttt ctccagtagc ctttggatca
     4801 ccgataaatc cagtcgctcc accaaccaac ataattgggt ggtgtccatg tttttctaaa
     4861 cgtttggcca tcaacaatga tgaatagtgt ccaatgtgta aactatctgc tgttggatct
     4921 gtaccaatat aaaatgtgac ttccccatta ttaattacat cttccacttc ttcacttgta
     4981 acattgtcaa ctagaccacg ccatttcaat tcttcaaata attccatttt gtcctccctg
     5041 aaaataaaaa aaatcgccca ataaggacga tttgatcgcg gtaccacctt acttcataca
     5101 aaatattttt tgtatgcact taaaccttta acgcaggtat acgtcaaaat tgaagctcca
     5161 aggtgtattc cacaggtcta tttgaaactt tacaccagcc agtttctctc taagaaatag
     5221 ataatctgtg tactctttct tatcatagca aatttattat atcgaatcta gaaactaaac
     5281 tcaacaaata tgttttaaat tgttataaat tttaaaaatg aatggtttac atggaaggat
     5341 gaagtttccg atatactcgt gtacgatcgc attaaatcca cgtttgaaat caaaaacacc
     5401 ataaccttct ttacccggtt caaaataacc actgatacca tagaaattat agaagtcata
     5461 gccctcttta atcgcttctt gaatcatatg ccaatgaatc gcataaggcc ctctaaaatc
     5521 tctttgttca taattgctag aaccaacaag atatacgatt tcatgaccat atttaatgaa
     5581 taaacaacac gcaagaggta cttctttacc atatgtatct tttaattctt ggatatgcga
     5641 aattttcttt tctaaagaat caagatatgt ttcatccgtc tttaaacgct tttccttttt
     5701 ctcatttccc ggattttttt ctaagaaaac ctttgtcttt tcaatacttg tgaaagtcgt
     5761 attgtattct ttcttgattt tttgttcata ttcatctaaa tccaaataag ataagagtgt
     5821 ttcaacatga tcttccccat aaaatctata taagttttta aaataatcta aactcatggc
     5881 ttcaaaatct tgtcgtgcac tcgtttcctt ttccatagaa tctaaaatat gaagttgatc
     5941 cacatttaat tttcgaatat gcacattata cttttgggcc gttcttgtat ttcttcttgt
     6001 agcacttgaa aatgcattaa agatttcatc actcgtttta cctcttaaat caatagagga
     6061 acaaaaacga cattccttcg ataaatcata tccttgtttt aaaggaagtt gtaaaaatcc
     6121 agctgatttt aaattatcta caacatcata attatgaaaa ccattctcga caacatttcc
     6181 atctttatct ctttgtacaa gatcaatttc aggatctatt tcaagataaa ctacattctt
     6241 ctttttaaga tattgtttta aagaagaggt aaactttaca agtttgtccc tatctttgta
     6301 gtcggcaaga aatcctcttg cgacataaca atatttaaag actttcatta aaggaagata
     6361 cgcaagcatg cacgttgcgt atacttcctt ttcttggctt gcctgaataa attcaaccgt
     6421 ccaaccatca tgttcaaatt tcttggcatg aaatggggat tgttgaaata ccgccgtatc
     6481 cactgtatct aaatattcgc tatattgttg aatatttatt gtatcgattc gcatacttca
     6541 ccttcttagt tggcaacaat attaacaact ttacctttta caacgataac tttacgaatt
     6601 gtttttcctt caatttgacg ctttactgtt tcaagttcca tagcctgttt ttctaattct
     6661 tcattacttg aacctacagc cgccataaat ttaccacgaa ccttaccatt tacctgtaca
     6721 acacaagtga ttgtatcttc cactaatttt gcttcatcat aagttggcca aggctcatac
     6781 gcaatggtat cttcatgacc taataattgc cacatttcct caccaatatg tggtgtaata
     6841 caagataaca tcttcacgat accttcagca tatggcttgt aaatggtttc tgccttatat
     6901 gcatcattca caaaaatcat catttgtgag atcgctgtat tgaatcctaa tgattcataa
     6961 tcttctgtaa cttttttaac tgtctggtta tagactttat ctaatttacc atcatttgta
     7021 tctataactt taccagactc aataaataaa cgataaacac ggtcaatcca tcttctagca
     7081 ccatcacaac ctgccattga ccaaggctta ctttgttcta atggacccat aaacatttca
     7141 tataaacgaa gtgtatcggc tccatatttt ttaacgatat ctgaaggatt cactacaaat
     7201 ttaggataac gtttacccat cttgattcca ttttcaccta agatcatacc ttggtgaact
     7261 aattttttaa atggttcttt acatgcaaca attcccttgt catataagta gttgttccaa
     7321 atacgtgaat acatcaagtg acctaccgca tgttcaggac cacctacata taaatcgact
     7381 ggcatccagt gtttcaataa ttctggattt cctaattgtt ctttattttg aggatctata
     7441 taacgtaaat agtaccatga agatcctgca gaccctggca ttgtacttgt ttcacgaact
     7501 ccctttacac cattttgatt gtattgtttc caatctgtcg cattttcaag tggcgctttt
     7561 ccattgtgac ctttataatc ttcaagttca ggtaagatca aaggcaattc atcgtctgga
     7621 agtacataat ctgtaccatc ttctaaatgc acaattggta caggttctcc ccaataacgc
     7681 tgacgtgcaa agatccattc acggaaacga taattcaccg tttctttggc aatacccata
     7741 tccaccaatt ttttagtaat tgctactttt gcctcttcta cagtaagacc tgtaaattct
     7801 tctgagttga ttagcttgca gcccttacct aagtaatctt gtttttcaaa ggcatgctca
     7861 ctcacgtctt gtccttcaat aacttgaatc atatcaatat tatgggcaat cgcatattca
     7921 aagtcacgtt gatcatgact tggtacagcc atgacagcac cagtaccata gtttgctaat
     7981 acgaaatctc caataaagac ttccacttct ctaccattca ctggattgat tgccttgata
     8041 ccttctaagc ggcaaccaga tttaccttta tttaattctg tacgatccat ttccgatttc
     8101 gatacagttt cctggatata agcctttact tcatcttggt ttttgattct tggcattaat
     8161 tcttgcacta aatctccatc tggtgcaagg accataaatg taataccata gatagtttcg
     8221 atacatgttg taaagattga gaattctcct ccgcctacga tttcaaattg aacttcaaca
     8281 cctgttgaac gtccaatcca gtttctttgc atttcctttg tggattcagg ccaatcaatt
     8341 tcattcaaac cttctaaaag tctttccgca aaagcagcct ggtcaataca aagttgcttc
     8401 atatttttac gaataactgg atagccacca cgttcacttt taccatcaat aacttcgtca
     8461 tttgataaaa cagttcctaa ttcttcacac cagtttactg gcatatccac atattttgca
     8521 tatccatctt tatacaattg tttaaaaatc cattgtgtcc agtgatagaa atctggatcg
     8581 gatgtcgcaa tcatttttga ccaatcatag tcaaaaccta attcttttaa ttgatttgtg
     8641 aaagttttaa tattttcttg tgtaaatcca tttggattgt ttcctgtttg aacagcatat
     8701 tgttcagccg gtaaaccaaa tgaatcatat cccattgggt gaagtacatt atagccttgc
     8761 atacgtttca tacgtgaaac gatatctgta gctgtatatc cttcagggtg tcctgcatga
     8821 agtccgactc cacttggata tggaaacata tctaacgcat aaaattttgg ctttgaaaaa
     8881 tcccaaacat ccgttttaaa tgtttcgtgt tcatcccaat acttgtgcca tttcttttct
     8941 acttctaaat gatcgtacat attagcctcc tataaaaaaa taaaaaacgc cctttaaatc
     9001 taaggacgtt tgtacgcggt accaccttag ttcacaatct tcattgtgcc cctttaactc
     9061 tttatcgcag agaatacgga taacattact catcagctcc ttagcaagtt caaaggttgt
     9121 acaatactaa ttctcaccta cattagctct ctgtaaattg ccatttccat ttactactct
     9181 aattcatcgc tatgcatcaa ttttatcttg ttttttaagg ttcgtcaata acaacatcca
     9241 cgattgattt atcatcataa taaatcttca tacatacatt atcataagta cactccatat
     9301 aagtcgaata atcttgaaaa tgcacgcgtg tattcataat tgtctcatcc ttttctttga
     9361 tcaactcttc ctttgtatga cacaacttaa tgcgattatt acgataaatc atacttttcg
     9421 catgatctac aattgatagt tctagaatgt tttgacgatt tgcctttaaa agatacacat
     9481 tttgaatggt acttaatgtt aaaaaggaaa caatttgtag cacaagaaac aaacaaacca
     9541 aacttgtcca gctcgcatat ccattaatag atttcaaagt catagatctt attcttcttt
     9601 ttaaaccaca aataataacc atcttctttc ttataaaaat agcctaagtc aatttgttcc
     9661 atatagattt catagccagg actcttcaca atacgatgct tatcaaaata caagaccttc
     9721 ttttcatgat ttaaaatata acttagacag tccgattcta ttttcaaatc atttgattgt
     9781 gcaagttc
//
LOCUS       DBMDWL010000072         9719 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2477_length_9719_cov_2.436889, whole genome shotgun sequence.
ACCESSION   DBMDWL010000072 DBMDWL010000000
VERSION     DBMDWL010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_9719_cov_2.436889"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="ACZPDN_06195"
     CDS             complement(<1..519)
                     /locus_tag="ACZPDN_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1298248.1"
                     /translation="MFKSISYVIKENCSNFYRIFCIAKYELLSDMRDSKFGIFWNFAS
                     PAIQVFTYWLVFGIAWGRKAQTYKGITVEYLPWLVVAFSVWWYIRPCIVDGCSAVFSK
                     TNVITRMKFPVSVLPATICLRELFNHFVMLIITFVTLILSGWYPNLNWLWILYYMFCA
                     FMLGEAISLVLSV"
     gene            complement(663..1010)
                     /gene="rpoE"
                     /locus_tag="ACZPDN_06200"
     CDS             complement(663..1010)
                     /gene="rpoE"
                     /locus_tag="ACZPDN_06200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HIR1298249.1"
                     /translation="MKKSMIEVAYQVLTNNDGAMPFVDLWKDVSQEMGFSQTQFEDNI
                     AQFYTDLSLDGRFLNMSQNTWDLRSRHTYSESVMDTDSIAIDEDDDEEEESEIVEDGL
                     DEVEKEMEEEENE"
     gene            complement(1036..2670)
                     /gene="argS"
                     /locus_tag="ACZPDN_06205"
     CDS             complement(1036..2670)
                     /gene="argS"
                     /locus_tag="ACZPDN_06205"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR1298250.1"
                     /translation="MQSIEVELKQAIQSAVKKAFDLDLDVQNIVVETPKNKDHGDFSS
                     NVAMQLTRQLHQNPRMIAQSLLDAFSLEMVSNIEIAGPGFLNFTLSKDRFSTVISDVL
                     AQKENYGQQPSNGIKVDLEYVSANPTGSLHLGHARGAAWGDSCARIMKKAGYDVTREY
                     YVNDAGNQITNLALSLNARYRQAQGIPTEIGQDGYMGKDVEEKGYELAKEYPETYLEP
                     TEENLKFFRKEGIKFELDKIRKDLDRYRVHFDVWSSEQSIRDAGKVEAAINELNEKGY
                     VYEKDGALWFETTKFGDDKDRVLRKQDGSLTYLVPDIAYHNDKCQRGFDMLVDFFGAD
                     HHGYIPRLKASMTALGNDADKLHVDIIQMVRLVSNGEEMKMSKRLGNATTINELCDKV
                     GVDAARYFFVQRALDSHLDFDIELATKKSNENPVYYAQYAHARMCSIQKQAQDIELAT
                     SFEDLTNEKEIELMKSLQEFNKVIVETAQSRQVHKMCHYIQNLASKFHSFYNVCKVVD
                     RDNLELSSQRLALVKATQIVLANALECIGVEAVESM"
     gene            complement(2657..3043)
                     /locus_tag="ACZPDN_06210"
     CDS             complement(2657..3043)
                     /locus_tag="ACZPDN_06210"
                     /inference="COORDINATES: protein motif:HMM:NF020711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 family protein"
                     /protein_id="HIR1298251.1"
                     /translation="MTNQVHLFDKISNQTLYKGEAIIDFCANEGLLVSFSNDTHRFEW
                     RIWKKGLVIRSESDVLVNLTLRLNSQTKGHIETEFGQIDLDCHTSHYDVDENHIEVKY
                     SLIQGNEEQKFHFVLYINKEDTYAVN"
     gene            complement(3212..4411)
                     /locus_tag="ACZPDN_06215"
     CDS             complement(3212..4411)
                     /locus_tag="ACZPDN_06215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1298252.1"
                     /translation="MFDKTNETKVLRDPVHGYIHVKYQVIWDCINAKEFQRLHRIHQL
                     GGDFQIYHTAEHSRFSHSLGVYEITRRMCEEVDSITATLSEYEKIQVLCAALLHDLGH
                     GPFSHFFETLHKKHHEQMTCDLILDPETEIHKALVKEDESLPQNIVLILTHKHPKPMM
                     HQMISSQLDADRMDYLLRDAYETGTSYGNFDLERILRTLRVKNDSLCVKMSGMHSVED
                     YIMARYHMYWQVYLHPDAKSYEIMIQQFFKRYAIIRNIDVFEPLMNGELSNNDFYLMD
                     EHRMFYGFQQALASEDEILKDFANRILNRHLFEWIEDPSDQQMQDIKLKLIESGYDLD
                     YYYYEAISHSKAYLPYTENDENQIIWMLDSKNELVSLSSCSQIVKALLKMKNQTNKRI
                     YFPKEIL"
     gene            complement(4378..4797)
                     /locus_tag="ACZPDN_06220"
     CDS             complement(4378..4797)
                     /locus_tag="ACZPDN_06220"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR1298253.1"
                     /translation="MNKLNVVCGALVIDGKVMIAQRNYGSSQGFFEFPGGKVEENETK
                     EEALIREWKEECDIDIYDVRYLSSSIDYQDGYEIHLTCFTCTSNEKPTKLSVHSEYIW
                     TTPDHIYDYNFFKSDKMLVEALKEVWSCLIKPMKLKY"
     gene            complement(4784..5053)
                     /locus_tag="ACZPDN_06225"
     CDS             complement(4784..5053)
                     /locus_tag="ACZPDN_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1298254.1"
                     /translation="MRLDKYLKTARILKRREAAKELALQGRIWVNDRVAKPSTELKIG
                     DTIRIRFGARLLEIRVLDIQKQVSKQNAQLLFEIIHEERIEDEQA"
     gene            complement(5053..8448)
                     /gene="mfd"
                     /locus_tag="ACZPDN_06230"
     CDS             complement(5053..8448)
                     /gene="mfd"
                     /locus_tag="ACZPDN_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1298255.1"
                     /translation="MIFLKETILNKLQDNPIVQQMLQFGNYIGNLLPNEEALCLASAF
                     MQDKQTRIVVKKNRYEAQQLYQRLKPLINDTLLFVMEESLRVQAIASSPEDKQEQLYV
                     LTQLVKEDKPRIIICNTAAFFRYLPDKELFRNLCFKFEVDQEITMTELKQKLNRAGYT
                     KVNYVDRPCTYASRGGIVDVYCLEYDHPIRIEFFDTVIESIRIFHEETQRTIETIDSV
                     LINPATDLLFTDDQIEFLQKEIDSELEKQHEKLIQDDYDFLLDYIEKDKIALENYDID
                     PHLYIYFSYLKANQFMDYVQGHIIFSSQEEVENGYKKLNEETISFIQEMVESHQYLPK
                     YSMFHDVFAFEKEHNIQMFQDFMDFKNPIFSKIETLEKSDQPLIQILENLKGNTIYIS
                     LDEEHTKKVEEALKAYSIHKNIIFIEDDFYEGFMYEDISVYTQQELFHRPSQIKRYQR
                     TFKQGQILQNILELEPNDYVVHEQYGIGQYVGITTRKIKGKTLDYLHVIYNGGDELFV
                     PLSQFQLVRKYVSKEGVGIKLSQLGSNKWKKTKEKVSKKVEEIAARLVELYAKRNEDI
                     GFAFSKDDDLQKEFEDAFEYEATPDQIRATEEIKREMEKPKPMDHLLCGDVGFGKTEV
                     AMRAAFKAISNNKQVALLCPTTILSMQHYQTFVKRFENVGANIRVVNRFVATKEINQI
                     KKELEAGQVDIIIGTHKLLNKSFQYKNLGLLIIDEEQRFGVEHKERIKEMKNAIDVLS
                     LSATPIPRTLQMSLIGVRTISQLNTPPLHRHPIQTYVMENRKSVVKEIIQRELSRGGQ
                     VFYLYNHVSNIYSVAKNIQNMFPDAKVAVAHGRMEKNDIEQTMIDFEQEKYQILVCTT
                     IIETGLDIANANTMIIDDADRFGLSQLYQIRGRVGRREKIAYCYLLVQPQKELTEQAH
                     KRLKAIKEFTSLGSGYKVAMRDLTIRGAGDMLGPQQAGFIDDVGLDLYLEMLASAIKE
                     KQGKPMEDKKNDKVAQVQLSGYIPEKFTDNDGDKLEIYQHIKKTESLTELIEYEKRVE
                     DLFGHIPNEVKQLFEQKKLDFFVNREGVESLKETEDVVTITMSVEWSKNCDGVKLFGA
                     INDISRKINLKLESGKIKIMISKRLKKHIELMMQVIDKLEGEF"
     gene            complement(8452..9012)
                     /gene="pth"
                     /locus_tag="ACZPDN_06235"
     CDS             complement(8452..9012)
                     /gene="pth"
                     /locus_tag="ACZPDN_06235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1298256.1"
                     /translation="MKIIIGLGNIGREYENTRHNAGFMAMDKLAQMLEMDFNQEKFSA
                     YFSKKKIDGEDIILLKPTTYMNNSGIALRQCMDFYKVSAEDILVLYDDMDMPVGKLRL
                     RQKGSAGGHNGIKSIISHIGTQEFDRIRIGIGKDKMIPVVDWVLGKFPQEQQEDLNHA
                     LEQAAKAAKFSIKHSFSDTMNRYNKK"
     gene            complement(9049..9675)
                     /locus_tag="ACZPDN_06240"
     CDS             complement(9049..9675)
                     /locus_tag="ACZPDN_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Jag family protein"
                     /protein_id="HIR1298257.1"
                     /translation="MEFAKFTGKTLDEALNVACNAKGCAKEELHYEVTEEKSGFLGIG
                     KTIEIEAYCQKDIEDFIKSYIQTYFDNAELDGTVEIENDHGFYRITVNTKNNAIMIGK
                     AGKSLQAFNRLVKAAVSAAFKKRVGLLIDVNGYKEERYEKICKMAVRVAKDVRRTKVD
                     AVLDPMPADERKAIHNALAGMNDISTKSEGEGEGRRLHILYTPGKDEE"
BASE COUNT         2989 a         1867 c         1246 g         3617 t
ORIGIN      
        1 tacagataat acaagtgaaa tcgcttctcc aagcataaat gcgcagaaca tataatatag
       61 aatccaaagc cagtttagat ttggatacca tccacttaaa atcaaagtaa cgaatgtgat
      121 aattaacatc acaaagtgat tgaatagttc tcttaaacaa atcgttgctg gtaaaactga
      181 caccggaaac ttcattcttg tgatgacgtt tgtctttgaa aatacggcag aacatccatc
      241 tacaatgcat gggcgaatat accaccatac actaaatgct acaacaagcc atggcaaata
      301 ttcgacagta atacctttgt atgtttgtgc ttttcttccc caagcaatcc caaagactaa
      361 ccaatacgtg aacacttgaa tagctggaga tgcaaagttc caaaaaatac caaatttaga
      421 atctcgcata tctgacaata attcatattt tgcgatacag aaaatacgat agaaatttga
      481 acaattttct tttataacgt atgaaatgct tttaaacata taaatatctc cttaacaact
      541 tctaggttat catatttttt ctagaaaaaa aatctgcatg aagcagattt tcttgcattc
      601 cttctccttt ccacttcaat tctaacaatt caatgtgaat ttattgtgaa aaaggatgcg
      661 ttttattcgt tttcttcttc ttccatttct ttttctactt cgtctaatcc atcttcaacg
      721 atttctgatt cttcttcttc atcatcatcc tcgtcaattg cgatagaatc tgtatccatt
      781 acactttctg aatacgtgtg acgtgaacgt aaatcccaag tattttgtga catattcaag
      841 aaacggccat ctaaagataa atctgtataa aattgtgcga tattatcttc aaactgtgtt
      901 tgactaaatc ccatttcttg acttacatct ttccaaagat caacgaatgg catagcacca
      961 tcgttgttag tcaatacttg ataagctact tcaatcatag actttttcat aatactataa
     1021 aactcctctt aatacttaca ttgattcaac ggcttcaaca ccaatacatt ctaatgcgtt
     1081 agctaaaaca atttgagttg cctttactaa agctagacgt tgtgaactta attctaaatt
     1141 atcacgatct acaactttac atacattata gaaactgtgg aatttactag ctaaattttg
     1201 aatatagtga cacattttat gaacttggcg agattgtgct gtttctacaa taaccttatt
     1261 gaattcttgt aaagatttca tcaattcaat ttctttttca tttgttaaat cttcaaatga
     1321 tgttgctaac tcaatatctt gagcttgttt ttgaatagaa cacatacgag catgtgcata
     1381 ttgtgcatag taaacagggt tttcatttga ctttttagtt gccaattcaa tatcgaaatc
     1441 taaatgtgaa tctaatgcac gttgtacgaa gaaataacga gctgcatcaa cgcctacttt
     1501 atcgcacaac tcattaattg ttgtcgcatt tcctaaacgt tttgacatct tcatttcttc
     1561 cccatttgat actaaacgaa ccatctgaat aatatctaca tgtaatttgt ctgcatcgtt
     1621 tcctaaagct gtcatactcg cttttaaacg aggaatatac ccatggtgat cagctccaaa
     1681 gaagtcaacc aacatatcaa atccacgttg acatttatca ttatgataag caatatctgg
     1741 aactaaataa gttaatgatc cgtcttgttt tcttaaaaca cggtctttat catctccaaa
     1801 ttttgttgtt tcgaaccata aagcaccatc tttttcatat acatagccct tttcattcaa
     1861 ttcattgatt gcagcctcta ctttacctgc atcacgaata ctttgttcac ttgaccaaac
     1921 atcaaaatgt acacgataac gatctaaatc ttttctaatt ttatctaatt caaacttaat
     1981 tccttctttt ctaaagaatt tcaaattttc ttctgtaggt tctaagtatg tttcaggata
     2041 ttctttcgcc aattcatatc ctttttcttc aacatcctta cccatgtaac cgtcttgacc
     2101 aatttctgta ggaattcctt gtgcttgacg ataacgagca tttaaagaca atgccaagtt
     2161 tgtgatttgg ttacccgcat catttacata atattcacga gttacatcat atcccgcttt
     2221 tttcatgata cgtgcacaac tgtctcccca agcagctcca cgagcatgtc ctaagtgtaa
     2281 agatccagtt gggtttgcac ttacatattc taaatctact ttaattccat tagatggttg
     2341 ttgaccgtaa ttttcctttt gtgctaatac atcagaaatt actgtactaa aacgatcttt
     2401 agataatgta aagttcaaga aaccaggtcc tgcaatttca atattagaaa ccatttctaa
     2461 tgaaaatgca tctaataaac tttgcgcaat cattcttgga ttttgatgta attgacgagt
     2521 taattgcatc gcaacattac ttgaaaaatc accatggtct ttattctttg gtgtttcaac
     2581 aacaatattt tgcacatcta aatctaaatc aaatgctttt tttactgcac tttgaatggc
     2641 ctgctttaat tcaacttcaa ttgactgcat atgtatcctc cttatttata tatagtacaa
     2701 agtgaaattt ttgttcttca ttcccctgta taagactgta ttttacttct atatgatttt
     2761 cgtccacatc ataatgactt gtatgacaat ccaaatctat ttgtccaaat tctgtttcaa
     2821 tatgcccctt tgtttgacta tttaaacgaa gtgtcaaatt cacaagaaca tcagattcac
     2881 tacggattac taatcctttt ttccatattc tccattcaaa tcgatgtgta tcattactaa
     2941 aagaaactag aagtccttcg ttcgcacaaa agtcaattat agcctctcct ttgtataatg
     3001 tttgatttga aattttatca aataaatgta cctgatttgt catagctagt gcataatatc
     3061 acactcagat ttttttgcaa gaaaatttat taaaaaacat aagaatttaa taaaaatatg
     3121 acaaatttaa tttaaaatct atgatttttt atatatttat cccatttttt cacaagtgaa
     3181 aacggtaaca cgtcgcatgt taccgttcat tttaaagtat ttcttttgga aaataaattc
     3241 gtttattcgt ttgatttttc atctttaata atgccttgac aatctgtgaa caactagata
     3301 aagacaccaa ttcattttta gaatctaaca tccaaatgat ttgattttca tcattttctg
     3361 tatatggcaa atacgcctta gaatgtgata ttgcttcata atagtaataa tctaaatcat
     3421 atccagattc aataagtttc agtttgatat cttgcatttg ttgatcactt ggatcttcga
     3481 tccactcaaa caaatgacga tttaaaattc gatttgcgaa atcctttaat atttcatctt
     3541 cgcttgctaa tgcttgttga aaaccataga acatacggtg ttcatccatt aaataaaaat
     3601 cattgttcga tagttctcca ttcattaatg gttcaaacac atcaatattt cggattattg
     3661 catatcgctt aaaaaattgt tgaatcataa tttcatatga ttttgcatct ggatgcaagt
     3721 aaacctgcca atacatatgg tatcttgcca taatatagtc ttcaacactg tgcataccac
     3781 tcattttcac acacaacgaa tcatttttca ctcgcaacgt tcgcaaaatt ctttctaaat
     3841 cgaaattacc atagcttgta ccggtttcat aagcatctct taaaagatag tccatacgat
     3901 ccgcatccaa ttgtgagctg atcatttgat gcatcattgg tttgggatgt ttatgcgtta
     3961 aaatcaacac aatattttgt ggtagggatt catcttcttt tactaaagcc ttatgaattt
     4021 ctgtttctgg atccaaaatc aaatcacaag tcatttgttc gtgatgcttc ttatgaagtg
     4081 tttcaaaaaa atgcgaaaaa ggaccatggc ccaaatcatg caacaatgca gcacataaaa
     4141 cttgaatttt ttcatattca cttaaagtag ctgtgattga atccacttct tcacacattc
     4201 gtcttgtgat ttcatatacg cctagactat gtgaaaaacg tgaatgctct gccgtatgat
     4261 agatttgaaa atctccacct aattgatgaa tgcgatgcaa tctttgaaat tcttttgcgt
     4321 tgatacaatc ccatatcact tgatatttca catgaatata tccatgcaca ggatctctta
     4381 atactttagt ttcattggtt ttatcaaaca tgaccatacc tcctttaatg cttctacaag
     4441 cattttatca gatttaaaga agttatagtc atatatatga tctggagttg tccaaatata
     4501 ttctgaatgc acacttaatt ttgttggttt ttcattagaa gtacatgtga aacaagttaa
     4561 gtgaatttca tatccatctt gataatcaat acttgatgat aaatatctaa catcataaat
     4621 atcaatatca cattcttctt tccattcacg aattaatgcc tcttcttttg tttcattttc
     4681 ctcaactttt ccaccaggaa attcaaagaa cccttggctt gaaccataat ttctttgtgc
     4741 aatcatgact tttccatcaa tcacaagagc tccacacaca acattaagct tgttcatctt
     4801 caattctttc ttcgtgaatg atttcaaaca acaactgtgc gttttgttta gaaacttgtt
     4861 tttgaatatc taatacacga atttcaagca gtcttgctcc aaaacgaata cgaatcgtat
     4921 ccccaatttt taattccgtg cttggtttag ccactcgatc attgacccag attcgtcctt
     4981 gtaaagctag ttctttagct gcttctcttc tttttaagat tcgtgctgtt tttaaatatt
     5041 tatctaatcg catcaaaact ctccttctaa tttatcaatc acttgcatca tcaattcaat
     5101 atgcttcttt aatcgtttag aaatcatgat tttaattttt ccactctcta atttcaaatt
     5161 gatctttcga ctaatatcat tgatggcgcc aaatagtttt acaccatcac aattctttga
     5221 ccattcaaca ctcatagtga tagtaacaac atcttctgtt tcttttaatg attcaacacc
     5281 ttctcgattc acaaagaaat ccagtttttt ctgttcaaac aattgtttta cttcatttgg
     5341 aatatgtcca aataagtctt cgacacgctt ttcatattca atcaattcag taagactctc
     5401 tgttttcttg atatgttgat aaatttctag tttgtctcca tcattatccg taaatttttc
     5461 aggtatatat cctgataatt gcacttgagc tactttatca tttttcttat cttccattgg
     5521 cttaccttgt ttttctttaa tagcacttgc caacatttca agatataaat ccaaaccaac
     5581 atcatcaata aatcctgctt gttgaggacc aagcatatct ccagctccac gaatcgttaa
     5641 atctcgcata gccactttat atccactgcc taaagatgta aattctttaa tggcttttaa
     5701 tcttttatga gcttgttctg ttaattcttt ttgtggctgc accaataaat aacaatatgc
     5761 gattttttct cgacgcccca cacgaccacg aatctgatac aattgcgata atccaaaacg
     5821 atccgcatca tcaatgatca ttgtattcgc attggcaata tcaagtcctg tttcaataat
     5881 tgtcgtacat actaaaattt gatatttctc ttgttcaaaa tcaatcatag tttgttcaat
     5941 atcatttttt tccatacgcc catgcgctac tgccactttt gcatctggaa acatattctg
     6001 aatatttttt gcgacagaat agatatttga aacatgattg tacaaataaa atacttgtcc
     6061 accacgtgat aattctcttt gaataatttc tttgacaaca ctttttcgat tttccatcac
     6121 atatgtttga ataggatgtc gatgtaaagg aggcgtattc aattgtgaaa ttgtacgcac
     6181 gccaatcaaa gacatctgta atgttcttgg aatgggtgtt gcactcaaag atagaacatc
     6241 aatcgcattt ttcatttctt taattctttc tttatgttca acaccaaatc gttgttcttc
     6301 atcaatgatt aataaaccca aatttttata ttggaaagat ttattcaaca atttatgagt
     6361 accaataata atatctactt gacctgcttc taattctttt ttaatttgat tgatttcttt
     6421 tgtcgccaca aaacgattca caacacgaat atttgctccc acattttcaa atcttttaac
     6481 aaatgtttga tagtgctgca tagataaaat agttgttgga cataataaag ctacttgttt
     6541 attgttcgaa atcgccttaa aggcagctct catcgcaact tccgtttttc caaatccaac
     6601 atctccacaa agtaaatgat ccattggttt tggtttttcc atttctcgtt tgatttcttc
     6661 tgtagctcta atttgatctg gcgttgcctc atattcaaag gcatcctcaa attctttttg
     6721 tagatcatca tctttactaa acgcaaatcc aatatcttca tttcttttgg catataattc
     6781 aaccaagcga gcggcaattt cttcaacttt tttagaaact ttttcttttg tttttttcca
     6841 cttatttgaa cctaattgag atagttttat gcccacccct tctttagata catattttct
     6901 tacaagctga aattgtgata atggtacaaa taattcatct ccaccattat atataacgtg
     6961 caaataatct aaggtttttc cttttatttt ccttgttgtg atcccaacat attgaccaat
     7021 accatattgt tcatgcacaa catagtcgtt tggttcaagt tcaagaatat tttgcaaaat
     7081 ttgtccttgt ttaaatgttc tctgatatct cttaatttga ctagggcgat gaaataattc
     7141 ttgttgagta tacacagaaa tatcttcata catgaaccct tcataaaaat catcttcaat
     7201 aaatatgata tttttatgaa tcgaatatgc ttttaaagct tcctctacct ttttagtatg
     7261 ctcttcatcc aatgaaatat agattgtatt tccttttaga ttctctaaaa tttgaatcaa
     7321 tggttgatct gatttttcaa gtgtttctat ttttgaaaag atgggatttt taaaatccat
     7381 gaaatcttga aacatctgaa tattatgttc tttttcaaat gcgaatacat catggaacat
     7441 actatattta ggtaaatatt gatgggattc aaccatttct tgtataaatg aaattgtttc
     7501 ttcatttaat tttttatatc cattttctac ttcttcctga ctcgaaaaaa taatatgacc
     7561 ctgcacataa tccatgaatt ggtttgcttt taaataacta aaataaatgt ataaatgagg
     7621 atctatatca taattttcta aagctatttt atctttttca atataatcca ataaaaaatc
     7681 ataatcatct tgaataagtt tttcgtgttg tttttccaat tcagagtcaa tttccttttg
     7741 tagaaattct atctgatcgt ctgtaaataa taaatctgta gctggattaa ttaatacaga
     7801 atcaattgtt tcaatggttc tttgtgtttc ttcatgaaag attcgaatgg actcaatgac
     7861 tgtatcaaaa aattcaatac gaatcgggtg gtcatattca agacaatata catccacaat
     7921 accaccacga cttgcatatg tgcatggacg atctacataa ttcactttag tatagcctgc
     7981 gcgatttaat ttttgtttca attcagtcat tgtgatttct tgatccactt caaatttgaa
     8041 acaaagattt ctaaataatt ctttatctgg taaataacgg aaaaaggccg ccgtattgca
     8101 tatgataata cgtggcttat cttctttcac taactgtgtt aaaacataga gttgttcttg
     8161 tttatcttct ggacttgaag caatggcttg aactcgtaaa gattcttcca tcacaaataa
     8221 taatgtatcg ttaataagtg gtttcaatct ttgatagagt tgttgcgctt catagcgatt
     8281 ctttttaact acaatccttg tttgtttatc ttgcataaac gcactagcta aacacaatgc
     8341 ttcttcattt ggaagtaagt ttcctatata atttccaaat tgaagcattt gttgaacgat
     8401 aggattgtct tgtagtttat ttaatattgt ctctttcaag aatatcacct tctacttttt
     8461 attatatctg ttcattgtat cactaaaaga gtgtttaatt gaaaatttag ctgcttttgc
     8521 ggcctgttcc aaagcatgat ttaaatcttc ttgttgctct tgaggaaatt ttcctaatac
     8581 ccaatctaca accggaatca ttttatcttt tccaatacca atgcgaatac gatcaaactc
     8641 ttgcgtacca atgtgagaaa ttatactttt gatcccatta tgtcctccgg cactgccttt
     8701 ttgacgcaaa cgaagttttc caacaggcat atccatatca tcatataata caaggatatc
     8761 ttctgcggat actttataga aatccatgca ttgtcttaaa gcaataccac tattattcat
     8821 atatgtagtt ggctttaata aaataatatc ttctccatca atcttctttt ttgaaaaata
     8881 agccgaaaat ttttcttgat taaaatccat ttctaacatt tgtgctaatt tatccattgc
     8941 cataaatcct gcattatgac gtgtgttttc gtattctcta ccaatatttc ctaatccgat
     9001 aataattttc atgaagtcct ccttcaaaaa aataacggga taatcccgtt attcttcatc
     9061 ttttcctggt gtatataaaa tgtgcaaacg acgcccttcg ccttcacctt cactcttagt
     9121 agaaatatcg ttcattcctg ctaaagcatt gtgaattgct tttctttcat cagctggcat
     9181 tggatctaat acggcatcta cttttgtacg acgcacatct tttgcgacgc gaactgccat
     9241 tttacaaatt ttttcgtatc tttcttcttt ataaccattt acatcgatta ataggccgac
     9301 acgttttttg aatgctgcac taacagctgc ttttactaaa cgattgaatg cttgtaaaga
     9361 cttacccgct ttaccaatca taatcgcatt gttctttgta tttacagtaa tgcgatagaa
     9421 gccatgatca ttttcaattt caacagttcc atccaattct gcattatcaa aatatgtttg
     9481 aatataggat ttaatgaaat cttcaatatc tttttggcaa tatgcttcaa tttcaattgt
     9541 ttttccaatt cctaaaaaac cacttttttc ttctgtaact tcataatgaa gttcttcttt
     9601 tgcacatccc tttgcgttac atgcaacatt taaagcctca tctaaagttt tcccagtaaa
     9661 ttttgcgaat tccatatgta ttacctactt tttgttcata acttggtgta ggatcaatg
//
LOCUS       DBMDWL010000073         9389 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2601_length_9389_cov_3.472868, whole genome shotgun sequence.
ACCESSION   DBMDWL010000073 DBMDWL010000000
VERSION     DBMDWL010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_9389_cov_3.472868"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(121..1053)
                     /locus_tag="ACZPDN_06245"
     CDS             complement(121..1053)
                     /locus_tag="ACZPDN_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012435.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1298258.1"
                     /translation="MFYTLYFLYTPIIYAGVQNGIERVSKFMMPVLVVLSVIIAGYSI
                     SRPGAFEGVKYFLIPNFDNFSWMTVVSAMGQMFYSLSIAMGILITFGSYMKKDVSIEG
                     STENVEIFDTAIAIMAGLMIIPAVFSFSGGNMETLKAGPSLMFITIPKVFASMGLGTG
                     IGIIFFLLVLFAAITSSIALTESAVSTFQDELHWSRKKSTIILGIIMIGLGTLSCLGY
                     GPLSNVTIIGMQFLDFFDFLTNSVMMPIAAIAICIFVSRVVGLEKVEAEITKDGNSFK
                     RNKIFNFMIQYLCPIFAFIILLSSIANAFGFISM"
     gene            complement(969..1484)
                     /locus_tag="ACZPDN_06250"
     CDS             complement(969..1484)
                     /locus_tag="ACZPDN_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012435.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298259.1"
                     /translation="MHNKRSTFSGKLGFVLSAAGASVGLGNIWRFPYLAAKYGGGIFL
                     LIYIVLALTFGYTMIIAETTLGRMTKKSPVGAFYTFTKTKFASFSGWINAIIPILIVP
                     YYSVIGGWVIKYLFEYLKGDAKLLATDTYFSNFISNGVSTEICFILFTFFTLPSFMQG
                     FKTELKEYPNL"
     gene            complement(1636..1711)
                     /locus_tag="ACZPDN_06255"
     tRNA            complement(1636..1711)
                     /locus_tag="ACZPDN_06255"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1676..1678),aa:Glu,seq:ttc)
     gene            1861..2685
                     /locus_tag="ACZPDN_06260"
     CDS             1861..2685
                     /locus_tag="ACZPDN_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1298260.1"
                     /translation="MIRLLVDSASDYTQEEIENKNLYFVPLQIQIEGKNYLDGVDIDK
                     ETFYEKMMNSKEFFKTSQPSPQEFLYIFEKVKEKQQTLICLSLSSKLSGTYQSACLAK
                     DMVDYENIFIIDTLTAVAGIRLLCEVAMNWIGENRSVESIVSGLEELKEHVHIYAGLD
                     TLEYLAKGGRISKTAAAIGTLASIKPIICVDHGEVVVAGKTRGVNKATSVVCEKMQSD
                     IVDTLYPVYTLYSYGLENVEKLESKLDLDVNRVRIGSTIGVHIGPNAFGVCFVSKL"
     gene            2755..3669
                     /locus_tag="ACZPDN_06265"
     CDS             2755..3669
                     /locus_tag="ACZPDN_06265"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1298261.1"
                     /translation="MKTTLIIMAAGIGSRFGGGIKQLAKMGPNGEIIMDYSIYDAKEA
                     GFDKVVFIIRKDIEEEFKEVIGNRIQNYMDVEYVFQDINDLPEGFTCPEGRTKPWGTG
                     QAVLCAKSVVDTPYVIINADDYYGKEAFVKLHDFLINHEKMGKEFDMGMAGFILKNTL
                     SENGTVTRGVCKVDDNNLLKEVYETTGIKAEAEKIVCDDPEVEAWAKPESNVSMNMWA
                     GYPDFLDYLEKDFSTFLSNISDNPMKKEYLLPNIVAELLREDRINVKVLETHDKWFGV
                     TYAEDKEYVQNAFKQLIENGVYPEKLWK"
     regulatory      3702..3803
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            3862..5169
                     /locus_tag="ACZPDN_06270"
     CDS             3862..5169
                     /locus_tag="ACZPDN_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1298262.1"
                     /translation="MKKEDVKKGSIYELDGVVPLKEALPLGIQHVLAMFLGNISPLLI
                     IAGMLGLSPDLKSALIQNAMFIAGAVTLVQLYPVWKVGAKLPIVMGTSSGFIGTAKAM
                     GALYGYGALMGASLIGGLFEMVLGFFIKPLRKLFPPVVTSLVIISIGLSLLPVGINYF
                     GGGNGAADFGDPKHLLVGTFVILVIIIAKQIKGFVNNASILIGIIAGYILAIVLGMVD
                     FTQVQTASWIALPTFMPVKMEFNLEVIIAMGIMFIATTVETVGDVSGITNGGLDREAT
                     SEELQGGVLADGLGSVVASLFGVLPNTSFSQNVGLVSVTKVVNRFTIMTGAIFLILCG
                     FCPKLSALFAVMPQSVLGGAAVIMFASILVSGIQSLMRVEFNERNNLIIALAIGLGIG
                     IGQVPTVLAQLPSWVGNIFAQNGIIMTFVIATVLNLILPGKKD"
     gene            5247..5996
                     /locus_tag="ACZPDN_06275"
     CDS             5247..5996
                     /locus_tag="ACZPDN_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR1298263.1"
                     /translation="MLRIMNYATPESLDEAYALLTKNKKNMILGGMIWLKMEDIQIPT
                     AIDLSKLGLDQIEEDDAEFKIGAMVTLRQLETHESFNKATCYVFRDAVKDIVGVQLRN
                     LATIGGSVYSRFSFSDVICSLLCLECDVQTHAHGRISLEEFISLPYEKDIVEYIYVKK
                     TNLPSAFVSVRKSATDLSVLNASCTKYADHYRFCFGARPAIAKVYNCDLDHIDLDVYC
                     ADNMRGSKEYREKLVEGLTQKLLRKVESYVG"
     gene            5986..6447
                     /locus_tag="ACZPDN_06280"
     CDS             5986..6447
                     /locus_tag="ACZPDN_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR1298264.1"
                     /translation="MLVKIKVNGKLIQDDVQPQTLLLDWLRDHGCYSVKRGCDTANCG
                     LCTVWKDGTPILSCSTLAVRCDGHEITTLEGLQKEASEFGMFMAKQGAEQCGFCSPGF
                     IMNVLAMEKELHDPSDDEIRAYLAGNLCRCSGYMSQMRAIKAYLSRGENHE"
     gene            6440..8716
                     /locus_tag="ACZPDN_06285"
     CDS             6440..8716
                     /locus_tag="ACZPDN_06285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR1298265.1"
                     /translation="MNSVGKGVIKKDAMALVTGQPVYCDDLAPKDCLIVKLLRSPHAY
                     AKIKSIDTSIAKRIPGIEAVYTYEDVPTSRFTLAGQSYPEPSPYDRRILENVVRYVGD
                     EVAIVAGANEDCVDRALKAIKVDYEVLEPLLDFEKSIDNTIVVHEEEDYKCLCEIGND
                     VKRNIVSSGESVVGDVDAVLKECDVVLERDYHTKANAQAMMETMRSYCYIDTYGRLNC
                     VSSTQIPFHVRRILSNALEIPKSKINVIKPRIGGGFGAKQTACCEIFTAFVTWKLKKP
                     SKIVYTREETFAASNSRHEMKMHVRIGATKDGTIEAIDLYTLSNQGAYGEHGPTTIGL
                     AGHKSLPLYNHVKASRFTYDVVYTNTMRAGAYRGYGATQGQFAVESIINELADELNMD
                     PCEIRFKNMTRENEVLSQYYNEELNACALDRCLEKAMEMIDYKNKPLRRDMGDFVRGL
                     GVSLSMQGSGISGLDVGSVEIKLQDDGFYTLSIGATDMGTGCDTILAQMVAECMDCDV
                     DQVVTSSLDTDHAPYDTGSYASSTTYVTGMAVVKACEKLRNSILEAAVGFFNVDKEDI
                     EFDGKKINTLDHVHEMSLAEFADTCFNGGIAKALIASDSHMSPTSPPPFMVGIAEVDV
                     DKLTGEIKVHDYVSVVDCGTVINPNLARVQAEGGIVQGIGMALSEDITYSNEGKMRNR
                     NFLQYKLPTRVDVPSVRVEFESSYEDNGPFGAKSIGEVVINTPTSAIASAIKHATGVQ
                     VRTLPITAEKVLLGKEDE"
     gene            8709..9248
                     /locus_tag="ACZPDN_06290"
     CDS             8709..9248
                     /locus_tag="ACZPDN_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1298266.1"
                     /translation="MNNISLIILASGFSRRFVSNKLLYEFHGKKLIEYAFDKASAFLD
                     VIVVTQYEKIKDMAEEQGYRVVMNDHPEYGQGHSIALGVGACQNNACILMVADMPYLK
                     LDTLKEMASIQDGKHIVICEYNGILCNPMLLPECYYEKAKQLCNDRGAKQIIGNDMYL
                     TVSLSEEEFMDIDEKTEAI"
BASE COUNT         3034 a         1284 c         2002 g         3069 t
ORIGIN      
        1 gaaacgtcga ctatttttgg gtttatgaag tacgtttctg ccacagatat tctatcagat
       61 tttaaaaaga ctgtcgacag tcactttgtc aacagtctga aagcttagca caagctaagc
      121 ttacatggaa ataaatccaa atgcgttcgc aattgaactt aataaaataa tgaatgcgaa
      181 aattggacat aagtattgaa tcatgaaatt gaatatttta ttacgtttga atgaatttcc
      241 atcttttgtg atttctgctt cgactttttc taatccaaca actcttgaaa caaagataca
      301 aatcgcaatg gcagcgattg gcatcatgac tgagtttgtc aagaaatcaa agaagtctaa
      361 aaattgcatt ccgatgattg ttacgtttga caatggacca taacctaaac atgatagtgt
      421 tcctagtcca atcataataa tgcctaaaat gattgtagat ttttttctag accaatgtaa
      481 ttcatcttgg aatgtggata cagcactttc ggttagcgca atagagcttg tgatagctgc
      541 gaataacacc aacaagaaga aaatgatacc gatacctgta cctagtccca tacttgcgaa
      601 tactttaggg attgtgatga acatcaagga tggtccggct tttaatgttt ccatgtttcc
      661 acctgaaaaa gaaaatacag ctggaataat catcaatcct gccataatcg caatggctgt
      721 atcgaaaatt tctacgtttt ctgttgaacc ttcaatggaa acgtcttttt tcatataaga
      781 accaaatgtg atcaagatac ccatcgcaat ggataacgaa tagaacattt gtcccatagc
      841 acttactacg gtcatccatg aaaaattatc aaaattagga attaagaaat atttaacacc
      901 ttcaaatgcg ccaggtcgac tgattgaata tcctgcaatg attacagata atacgacaag
      961 aactggcatc ataaatttgg atactctttc aattccgttt tgaacccctg cataaatgat
     1021 gggagtgtaa agaaagtaaa gagtataaaa catatttctg tagatactcc gtttgaaatg
     1081 aagtttgaaa aatatgtatc tgtggcaagt aattttgcgt cgccttttaa atactcaaat
     1141 aaatatttga tgacccaacc tccgattaca gaataatagg gtacaatcaa aattggaata
     1201 atggcattga tccatccgga aaatgacgca aactttgtct ttgtgaaagt ataaaaagct
     1261 cccactggac ttttctttgt catgcgacct agtgttgttt cggctatgat cattgtataa
     1321 ccaaaagtta aggcaagaac aatgtaaata agtaagaaga ttcctcctcc gtatttagcg
     1381 gctaaatagg ggaatctcca tatattgcca aggccaacac tggctcctgc ggcagacaag
     1441 acaaatccta atttgccaga aaatgtacta cgtttattgt gcataataaa aacacccttc
     1501 ctcaataatt tagattatat catctatgaa agggtttgcg caattttatg aaaagcattt
     1561 gtgaaatttt cttaatattt ttgaaaaaac tgttttggat aaaaaaagaa gcccttacgg
     1621 actttgtttg aaaaatggtg acgcgtacgg gattcgaacc cgtgagtgca tgcgtgaaag
     1681 gcatgtgagt taaccgtttc tccaacgcgc catgtatatt aaacttgccc ttcttgacaa
     1741 gtgctcacta atattactac aacattttaa ttaatgcaag tatttttttt aaaaaatttg
     1801 acagatttat ttaatagtat ataatgtgaa cagttaacat agttaacggc ggaggaaatt
     1861 atgattcgat tattagtaga ttcagcaagt gattatacgc aagaagaaat tgaaaataaa
     1921 aatttatatt ttgtgccttt acaaattcaa attgaaggaa agaactattt ggatggtgtt
     1981 gatattgata aggaaacatt ttatgagaag atgatgaatt caaaagaatt ttttaaaaca
     2041 tcacaaccat caccacaaga atttttatat atttttgaaa aagtgaaaga aaaacaacaa
     2101 acattaatat gtttatcctt atctagtaaa ttgagtggaa cctatcaaag tgcttgtttg
     2161 gcaaaagata tggtggatta tgagaatatc tttattattg atacattgac agcagttgca
     2221 ggaattcgtt tactatgcga agtggcaatg aactggatcg gtgaaaatag aagtgttgag
     2281 agtattgtat ctggtttaga agaactaaaa gagcatgtgc atatttatgc tggtttagat
     2341 acattggaat atttagctaa aggtggaaga atttctaaga cagcagcagc tattggaact
     2401 ttggcaagta ttaaacccat tatttgtgta gaccatggag aagttgtggt tgccggtaag
     2461 acacgtggtg taaataaggc aacgagtgtt gtatgtgaaa agatgcagtc agatattgtt
     2521 gatacattgt atccagttta tacattgtat tcatatggtt tagagaatgt tgaaaaacta
     2581 gaatcaaaac tggatttaga tgtgaatcgt gttcgtattg gttcaacaat tggagttcat
     2641 attggaccaa atgcatttgg tgtttgtttc gtatctaaac tatagttaac ttgtgaaggt
     2701 gtatacattt tagtataatg gatatacgga tttacaagga gagtttttga taatatgaaa
     2761 acaacattaa ttattatggc agctggaatt ggttctcgtt ttggtggtgg aatcaaacag
     2821 ttagctaaaa tgggtccaaa tggtgaaatc attatggatt attcaatcta tgatgccaaa
     2881 gaggctggct ttgataaagt cgtatttatt attcgcaagg atattgaaga agaattcaaa
     2941 gaagtgattg gaaatcgtat ccaaaattat atggatgtag aatatgtatt ccaagatatc
     3001 aatgatttac cagaaggatt tacttgtcct gaaggtcgta ctaagccttg gggaacaggc
     3061 caggctgtat tatgtgcgaa aagtgttgta gatacaccat atgtgattat caatgcggat
     3121 gattattatg gtaaagaagc atttgtgaaa ttacatgact tcttgattaa tcatgaaaaa
     3181 atgggaaaag aatttgatat gggtatggcc ggattcattt taaagaatac attaagtgag
     3241 aatggaactg taactcgtgg tgtatgtaaa gtggatgata ataatttatt aaaagaagtt
     3301 tatgaaacaa ctggaatcaa ggctgaagca gaaaaaattg tatgtgatga tcctgaagta
     3361 gaggcttggg caaaaccaga aagtaatgta tctatgaata tgtgggctgg atatccagat
     3421 ttcttagact atttagaaaa ggattttagt actttcttat caaatatttc agataatcca
     3481 atgaagaaag aatatttgct tccaaatatt gtagctgaat tattgaggga agatcgtatc
     3541 aatgtgaagg tacttgaaac acatgataaa tggtttggtg taacatatgc cgaagataag
     3601 gaatatgtgc aaaatgcctt taaacaattg attgaaaatg gtgtatatcc tgaaaaatta
     3661 tggaaatagg acgatttgtc ctattttttt tgttagatta atgatataat ccagacgtgt
     3721 ttcgtatatg tcgactaata cggtgtcgac gtttctaccc ctaaaccgta aattcaggga
     3781 ctatgaatca acatactatt tttattgtgt cgtgttgatt ctcgtttttg tgcgagactt
     3841 ttttattttc ggaggaaaag aatgaaaaaa gaagatgtga aaaagggttc gatatatgaa
     3901 cttgatggtg ttgtaccttt aaaagaggca ttgcctttag gaattcaaca cgtattggct
     3961 atgtttctag gaaatatcag tccattatta attattgcag gaatgctagg attatcaccg
     4021 gatttaaaat ctgcattgat tcaaaatgcg atgtttattg ccggagctgt tactttagtt
     4081 cagttgtatc cagtgtggaa agtgggagct aaacttccaa tcgttatggg tactagttct
     4141 ggatttattg gaactgccaa agccatgggc gctttatatg gttatggtgc attgatggga
     4201 gcaagtttaa ttgggggctt atttgagatg gttttaggtt tctttattaa accattaaga
     4261 aaattattcc cacctgttgt tacaagttta gttatcattt cgattgggtt atcattactt
     4321 cctgttggta ttaattattt tggtggtggt aatggtgctg ctgattttgg agatcctaaa
     4381 catttattgg ttggtacatt tgttattttg gtgatcatta tcgcaaaaca aatcaaagga
     4441 tttgtgaata atgcatccat tttgattggt attattgctg gatatatctt ggctattgta
     4501 cttggaatgg ttgattttac acaagttcaa acagcttctt ggattgcact tccaactttt
     4561 atgccagtaa aaatggaatt taacttagaa gtaattattg cgatggggat tatgtttatt
     4621 gcgacaacag ttgaaacagt cggagatgtt tctggcatca caaatggtgg tttagatcgt
     4681 gaagcaactt ctgaagaatt acaaggtggc gttttggcag atggtttagg ttctgttgtt
     4741 gcctcattgt ttggcgtttt accaaatact tcattctctc aaaatgttgg tttggtttca
     4801 gtgactaaag ttgttaaccg ttttacaatt atgacaggag caatcttctt gattctttgt
     4861 ggtttctgtc caaaattaag tgctttattt gcggttatgc ctcagtctgt acttggtgga
     4921 gcagctgtta ttatgtttgc gagtatttta gtgagtggta ttcaatcatt gatgcgtgtt
     4981 gaattcaatg aaagaaacaa tttgattatt gccttggcta ttggtctagg aattggtatt
     5041 ggacaagttc ctacagttct agcacaatta ccaagttggg ttggaaatat ttttgcacaa
     5101 aacggaatta ttatgacgtt tgtgatcgca accgtattaa atttaatttt gcctggcaaa
     5161 aaagactaat aaataggtgt taaacatttt gtttaacact ttctttttgt gttaaaattt
     5221 tagtaaagaa agagagcgta tccattatgt taagaataat gaattatgcg acgccagaat
     5281 ctttggatga ggcgtatgcg ctattaacaa aaaataagaa gaatatgatt ttgggcggta
     5341 tgatttggtt aaagatggaa gatattcaaa ttccaactgc tatcgatttg agtaaactag
     5401 gtttggacca aattgaagaa gatgatgctg aatttaaaat tggagccatg gtaactttac
     5461 gccaattaga aacacatgaa tcatttaata aagcaacttg ctatgtgttt agagatgccg
     5521 taaaggatat tgtcggcgta caattaagaa atttggcgac aattggagga agtgtatatt
     5581 cacgctttag ttttagtgat gtcatctgta gcttgttatg tttagagtgt gatgtacaaa
     5641 cacatgcaca tggtcgtatt tcacttgaag aattcataag tcttccatat gaaaaggata
     5701 tagtagaata catttatgta aagaagacga atttgccaag tgcctttgtt tcagtaagaa
     5761 aaagtgctac ggatttaagt gtattaaatg cgtcttgtac aaaatatgca gatcactatc
     5821 gtttctgttt tggggcaaga cctgccattg cgaaagtgta taactgcgat ttagatcata
     5881 ttgatctaga tgtatattgt gcagataata tgcgtggaag taaggaatat agagaaaaac
     5941 ttgttgaggg attaactcag aagttattga gaaaggtaga gtcttatgtt ggttaaaatt
     6001 aaagtgaatg gaaaattgat tcaggatgat gtacagccac aaactttatt gttagactgg
     6061 ctaagagatc atggttgtta cagcgtgaaa agagggtgtg ataccgcaaa ctgtgggttg
     6121 tgtacagtgt ggaaagatgg aacaccaatt ctttcttgta gtacattagc tgttcgatgt
     6181 gatggtcatg aaattacgac acttgaaggc cttcaaaaag aagccagtga attcggtatg
     6241 tttatggcta aacaaggggc tgaacaatgt ggtttttgtt caccgggatt tattatgaat
     6301 gttttggcaa tggaaaaaga attacacgat ccgagcgatg acgaaattcg tgcttattta
     6361 gccggcaatt tatgtcgttg ttctggatat atgtcgcaaa tgcgtgctat taaggcatac
     6421 ttaagtcgag gtgaaaatca tgaatagtgt aggaaaaggt gtcatcaaaa aggatgcgat
     6481 ggctttagtt acaggtcagc ctgtatattg tgatgattta gcacctaagg attgtttgat
     6541 tgtcaaactt ttaagaagtc cacatgcata tgcgaaaatt aaaagtattg acacttccat
     6601 tgcgaaaaga attcctggaa ttgaagctgt atatacatat gaggatgttc caacttctcg
     6661 ctttacattg gcaggtcaat catatcctga acctagtcct tatgatcgta gaatcctaga
     6721 aaatgtagtt cgctatgtag gagatgaagt ggcaattgtt gcaggagcta atgaggattg
     6781 tgtggatcgt gctttaaaag ctattaaagt agattatgaa gtattagaac cattgttgga
     6841 ttttgaaaaa tcgattgata atacaatcgt ggttcatgaa gaagaagatt ataagtgtct
     6901 ttgtgagatt ggtaatgatg taaagcgcaa tattgtcagc agtggagaaa gtgttgttgg
     6961 tgatgtggat gccgtattaa aagagtgtga tgtagtctta gaaagagatt atcatacaaa
     7021 agctaatgca caggcaatga tggaaacaat gcgttcatat tgttatattg atacatacgg
     7081 tcgtttgaac tgtgtaagtt ctacacagat tcctttccat gttcgtcgaa ttttaagtaa
     7141 tgctttagaa attccaaaat caaaaatcaa tgtgatcaag ccaagaattg gtggtggttt
     7201 tggggctaaa caaacggctt gttgtgaaat ttttactgca tttgttacgt ggaaacttaa
     7261 aaagccaagt aaaatcgttt atacccgtga agaaactttt gcggctagta actcaagaca
     7321 tgaaatgaaa atgcatgttc gtattggcgc tacaaaagat ggaacgattg aagcgattga
     7381 tttatataca ttatcaaatc aaggtgctta tggtgaacat ggtccaacta caattggttt
     7441 ggcaggacat aaatctttgc ctttatataa ccatgtaaag gcaagccgct ttacatatga
     7501 tgtagtttat acaaatacaa tgcgtgcagg tgcttataga ggatatgggg ctacacaagg
     7561 tcagtttgcg gttgaatcta ttattaatga attggctgat gaattgaata tggatccttg
     7621 tgaaatccgt tttaaaaata tgacgcgtga aaatgaagtg ttatcacaat attataatga
     7681 agaattgaat gcatgtgcat tggatcgttg tttagaaaaa gctatggaaa tgattgacta
     7741 caagaataaa cctttaagac gtgatatggg ggattttgtt cgtggtttag gtgtttcttt
     7801 atctatgcaa ggtagtggta tttcaggtct tgatgtaggt tctgttgaaa ttaaactaca
     7861 agatgatggt ttctatacat taagtattgg tgctacagat atgggtacag gatgtgatac
     7921 gattcttgcc caaatggttg cggaatgcat ggattgtgat gtagatcaag ttgtcactag
     7981 ttcattggat acagaccatg caccatatga tacgggttct tatgcatcaa gtacaacgta
     8041 tgtaacgggt atggctgtag taaaggcttg tgaaaaatta agaaattcta ttttagaagc
     8101 ggcagtcgga ttcttcaatg tggataaaga agatattgaa tttgatggta agaaaatcaa
     8161 tacattagat catgtgcatg aaatgagttt ggctgagttt gcggatacat gtttcaatgg
     8221 tggtatcgca aaagcattga ttgccagtga ctctcacatg tcgcctacaa gtccgcctcc
     8281 atttatggtt ggtattgcgg aagtggatgt agataagttg actggagaaa ttaaggttca
     8341 tgattatgtt tctgtagttg attgtggtac agttatcaat ccaaatcttg ccagagtaca
     8401 agctgaaggt ggtattgtac aaggtattgg tatggcttta agtgaggata tcacatactc
     8461 aaatgagggt aagatgcgta atcgcaactt cttgcagtat aagttaccaa caagagtaga
     8521 tgttccaagt gttcgtgttg agtttgaaag cagttatgaa gacaatggtc cttttggcgc
     8581 aaaatctatt ggtgaagttg ttattaatac gccaacaagt gcaatcgcta gtgctataaa
     8641 acatgcgacg ggagtgcagg ttcgtacttt gcctattact gctgaaaaag tcttattagg
     8701 aaaagaagat gaataatatt tctttgatta tcttagcgtc tggtttttcc agacgctttg
     8761 tttcaaataa acttttgtac gaatttcatg gtaagaaact tattgaatat gcttttgata
     8821 aggcaagtgc ttttttggat gtgatagttg tcacacaata tgaaaagata aaagatatgg
     8881 ctgaagaaca agggtatcga gtcgttatga atgatcaccc tgaatatggt caaggtcatt
     8941 ctatcgcttt aggtgtgggt gcttgtcaaa ataatgcatg catactgatg gttgcagata
     9001 tgccatattt aaaattggat acgttaaagg aaatggcttc cattcaagat ggaaagcata
     9061 ttgtgatttg tgaatataat ggtattttat gcaatcctat gttgttgcca gaatgttatt
     9121 atgaaaaggc taaacagtta tgcaatgata gaggtgcaaa acaaatcatt ggtaatgaca
     9181 tgtatttaac ggtttcttta tcagaagaag aatttatgga tatagatgaa aaaacagagg
     9241 ctatttagcc tctgtaatgt ttttctttgt ctttggcaat gtcaaaatag atttgttcgc
     9301 ctctttgttc ataaagcata tttgcccaat atccccaaag aatatcttcc attctttcaa
     9361 atgcctgcac ttgtacatca atcattgga
//
LOCUS       DBMDWL010000074         9290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2639_length_9290_cov_2.022200, whole genome shotgun sequence.
ACCESSION   DBMDWL010000074 DBMDWL010000000
VERSION     DBMDWL010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2639_length_9290_cov_2.022200"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(38..220)
                     /locus_tag="ACZPDN_06295"
     CDS             complement(38..220)
                     /locus_tag="ACZPDN_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298267.1"
                     /translation="MKRYVTYPDWVEKFRSPGHTIKKTKQGYGLYSCTSKYVPGGKPK
                     SIQTYLGKITPDGFIP"
     gene            390..1721
                     /gene="cysS"
                     /locus_tag="ACZPDN_06300"
     CDS             390..1721
                     /gene="cysS"
                     /locus_tag="ACZPDN_06300"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1298268.1"
                     /translation="MKLYNSYSQTIEELKPIEEGKISMYVCGPTVYNYPHIGNARPIV
                     VFDTLKKALSAQGYEVTFASNYTDVDDKIIKTAIEQNVSEKEITDKFIAAYNDVRRGL
                     HADLPDYAPRVTETMNRIIEFIDKMVKNGSAYQVGGDVYFRVNSDKKYGELSHQSIDD
                     LMVGARIDENEKKENPLDFTLWKETTEGIKWDSPWSKGRPGWHTECVVMIQDVFKKTL
                     IDIHGGGLDLKFPHHENEIAQCEVCNDTHLANIWVHNGMININGIKMSKSLGNVWWAK
                     DLIANHGGNLTRWLMISTHYRAPLNLNEEAFTTSKKELDKIAAAYKQACVKLALSSAQ
                     EATTFDETYTSFMDALNDDLNTPNAISVVYEVVKKINQAVRQREIDVENLAVLVHTLE
                     KMLYILGIEFDKVSMTNEDKEMYQNWRSAVKEKDFKTADTLRQTLIEKGIL"
     gene            1721..2107
                     /locus_tag="ACZPDN_06305"
     CDS             1721..2107
                     /locus_tag="ACZPDN_06305"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIR1298269.1"
                     /translation="MEIVTENATSLAWIGDAVMSLAVREHLLSLGFRKAHVLQKKNAV
                     ICSAKGQAVMLNKMMEEDFFTEDEKTILARGRNATIHSKAKNATHKTYLEATALESML
                     GYLYLYGHQERLKVCLDKIIEIGDSL"
     gene            2104..2820
                     /gene="rlmB"
                     /locus_tag="ACZPDN_06310"
     CDS             2104..2820
                     /gene="rlmB"
                     /locus_tag="ACZPDN_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1298270.1"
                     /translation="MIVYGKNVYSTLQNDMDSIEKVYVLQGLKDAKLLKSIQKSNLKV
                     EYCNRAKLDKIANTTHHNGVAIKVSEIETYSIEQIVSRAKKPGLLVALDGIQDPHNVG
                     AILRTCDCAGVDGVILTKHNSCGLTPTVVKASTGAAYTVPVSIVTNLSQTLRNLKQEG
                     YWVVGTDMKEAREYREGMYDSPTVLVIGSEGQGISNLVKKQCDYMVYLPMVGSITSLN
                     ASVAAAILMYEVYNQRNPKK"
     gene            2894..3490
                     /locus_tag="ACZPDN_06315"
     CDS             2894..3490
                     /locus_tag="ACZPDN_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298271.1"
                     /translation="MNIYESIYLIQMNDFYEMNHLIQYYRPMVTEIWADIFVRKTNTI
                     LCERDEFYRHADLLLYRCIFEYRMDRVLTFTSFYRRCTKNKGYDLLRQAIRKQCYLNQ
                     VCISFDQKVKEDTNLYYSGISTDDSLEVHEIVMDKLMWEHIKTRIMNLFGEEYVQIVD
                     LRMEGYTFKYIAQKLGLSCAKVNFMFEKVKKWYTTIDS"
     gene            3535..3684
                     /gene="rpmG"
                     /locus_tag="ACZPDN_06320"
     CDS             3535..3684
                     /gene="rpmG"
                     /locus_tag="ACZPDN_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1298272.1"
                     /translation="MADKVVLTCTECLSRNYTTEKNKKTNTQRLEIKKYCPKCGKHTL
                     HKETK"
     gene            3694..3891
                     /gene="secE"
                     /locus_tag="ACZPDN_06325"
     CDS             3694..3891
                     /gene="secE"
                     /locus_tag="ACZPDN_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1298273.1"
                     /translation="MSKEKKERTYSPSKVFKELKTVKWPTFKELMSSSALVIVFTVLF
                     GLYFFVCEILSSGLVKMIVGA"
     gene            3902..4447
                     /gene="nusG"
                     /locus_tag="ACZPDN_06330"
     CDS             3902..4447
                     /gene="nusG"
                     /locus_tag="ACZPDN_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1298274.1"
                     /translation="MSELKKEWYVVNTYVGQENRVKENLERRIKTMGLEDFLFQIVVA
                     EEKEIEYKNGKPVEKTHNLFSGYLLVQMIMTDEAWYIVRNTPGVTGFIGSSGKGAKPF
                     PVAQEEVDAVLRRLGRQDVNVQVDFEVGDRVEITNGAFKNSEGTIESMDEEKKEATVL
                     LILFGRETPTEIPYMDLKKVD"
     gene            4472..5242
                     /locus_tag="ACZPDN_06335"
     CDS             4472..5242
                     /locus_tag="ACZPDN_06335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR1298275.1"
                     /translation="MKTLVFDLDGTMYRGTQIIESAHQFLDYCIQNKIPFIFLTNNSM
                     RTPQENVKHMQDMGYTNIEPSMFYNSAMAACQYVKKHYKGNRAYYIGKQGMKDALDQE
                     GFIVDDENPDFVFIGLDKDATYKSYSKALSLILNGAKIIGTNNDRILAKPGGFEVGNG
                     SVVDMFEYAANQKSPRIGKPNRAILDLCLEYFGLKEDEIILIGDNLETDIKLGFEQNI
                     ETVLVQTGVHKKEDIEQLKIYPTHVVNTLMDLVDYNFD"
     gene            5298..5528
                     /gene="secG"
                     /locus_tag="ACZPDN_06340"
     CDS             5298..5528
                     /gene="secG"
                     /locus_tag="ACZPDN_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1298276.1"
                     /translation="MKDILNVVLMTLSGVIIILTLLQSGKSDGLSAAFTGSGDLNLFA
                     VQKERGPEKVISRLTLFCGILFFAIVIALQIV"
     gene            5574..7640
                     /gene="rnr"
                     /locus_tag="ACZPDN_06345"
     CDS             5574..7640
                     /gene="rnr"
                     /locus_tag="ACZPDN_06345"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR1298277.1"
                     /translation="MKEKIIQYLEEKKECKIRDLEKIVRPKSSKEFTEMVKSLNELED
                     EKLIYNDHTSLYLIDDIHFMAGSAKDISRYEIAVFNENNKVYVQKNKKMKIFDKDEVL
                     VRLKPEPSLIKVFNHGLTNITGVMIVKKKKTFFYSDLDLHCSIRVVNEKEFSFKKRTK
                     VVCQIVKYADPLEVRIIQVLGHPDDAGVDISAMLTSNQVRQVFNGKIVKECKRIPAKV
                     QKDELENRKDLRGLLTVTIDGDDAKDFDDAISIVKNEKGYLLYVHIADVSHYVKENSA
                     LDQEAYLRGNSIYVCDRVVPMLPFDLSNGICSLNPNVDRLTITCAMQVDEKGNLLTYN
                     IFPSVIHSDKRCTYKKVNACLDGDKTILQEYSEVKELLFTLESLAKLLKKQSHNRGCI
                     DFNTKEAKIIMDKNGYPVDVKVLERGFAEQMIEECMILANVCVAHHMHVNKIPSMYRV
                     HENPDPKKVATICTVANSLRIPFDFYPDEVNASQIQKFLESIQDENAYEVLSMVSLRS
                     MQKARYDAHCLGHFGLALEEYCHFTSPIRRYSDLVVHRMLHAYCFKEQKRTPSNDEKK
                     IEKQAFHISEKERDAIMMERMVNDYKKAQYMEKRVGQVFEGTIVGVMSFGFFVEMDNT
                     VEGLVPVHTLSNDFYDYDEDTMTLKGQTHGKVFQMGMRVRVVCTDVQREKGQVTFSLL
                     ERRNHD"
     gene            7781..7987
                     /locus_tag="ACZPDN_06350"
     CDS             7781..7987
                     /locus_tag="ACZPDN_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298278.1"
                     /translation="MVQTVGEGCIRHFAFATDYVDKIVDREFVKVSVEPKDVVMKTDP
                     VFLIRIDFIDGLLHESIELFCEKA"
     gene            8209..8880
                     /locus_tag="ACZPDN_06355"
     CDS             8209..8880
                     /locus_tag="ACZPDN_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR1298279.1"
                     /translation="MDQNFNKVFWDACANGDFPMVCSQIKAGADVNYQNGDGRTALMR
                     ASKRDRKDVVQVLLDNGADVNITDNKGKTALMTAAKKGNKTILKALIDAGADVNAKDD
                     RGRTALMRACLLGQDRCVEVLLDAGARINDQDEVGRSALMEACIAFKRDTIHLLLERN
                     ADVNLFDNNGVTALMRAAYGGYPSLVEKLLAYGADKDMTDKQGRVALDYVREHCRAQL
                     EPILR"
     gene            8891..>9290
                     /locus_tag="ACZPDN_06360"
     CDS             8891..>9290
                     /locus_tag="ACZPDN_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1298280.1"
                     /translation="MRDYQSFEVRNVSVIGHSGAGKTSVLEAMLYYTKAIDRFGKTTE
                     GSSMIDFDTEEIRRGLSVYTTIVPIEWEDSKINFIDTPGYLDFVGEKQCGFSVGDSSL
                     IVVNAKEPLQSGTKLAYKQAVAEKKPTIFFI"
BASE COUNT         3295 a         1166 c         1923 g         2906 t
ORIGIN      
        1 caaattcgac atagacagga tgtttagaaa cgacagacta aggaataaaa ccatcaggag
       61 tgatctttcc aagataagtc tgtatacttt taggttttcc accaggcaca tatttagaag
      121 tacatgaata aaggccataa ccttgttttg tttttttgat cgtgtgacca ggagaacgga
      181 atttttcgac ccagtcagga taagtaacat atcttttcat aataatagtg tacaccaata
      241 cactattaaa gtgtataaaa aaataaataa tcttaaatcg cgaaattttg aagatcattt
      301 catgcgtaat cactataacc tttgggttag cttttttttt cgagctttgt attgtataat
      361 aaactgtatg aattggagga cattttatta tgaaattata taatagttat tctcaaacaa
      421 ttgaagaatt aaaacccatc gaagaaggta agatttctat gtatgtatgt ggacctacag
      481 tatacaatta tccacatatt ggaaatgcac gtcctatcgt tgtatttgat acattaaaaa
      541 aagcattgtc tgcacaaggc tatgaagtga cttttgcttc aaactataca gatgtagatg
      601 ataagatcat caaaacggca attgaacaaa atgtgtctga aaaagaaatt acagataaat
      661 tcattgctgc ctacaatgat gtacgaagag gacttcatgc agatcttccc gattatgcac
      721 cacgtgtaac agaaactatg aatcgaatta ttgagttcat tgataagatg gttaaaaatg
      781 gttcagctta tcaagttgga ggcgatgttt atttccgagt taatagtgat aaaaaatatg
      841 gagaactatc tcatcaaagt attgatgatt taatggtagg agctcgtatt gatgaaaatg
      901 aaaagaaaga aaatccatta gattttactt tatggaaaga gacaacagaa ggtattaaat
      961 gggattcacc ttggtcaaaa ggacgtccag gctggcatac agaatgtgtt gtaatgattc
     1021 aggatgtatt caagaaaaca ttgattgata ttcatggtgg tggattggat ttaaaattcc
     1081 ctcatcatga aaatgaaatt gcccaatgtg aagtatgcaa cgatacacac ttggcaaata
     1141 tctgggtaca caatggtatg atcaatatca atggtattaa aatgtctaaa tctttaggaa
     1201 atgtatggtg ggctaaagat ttgattgcca atcacggagg taatttaact cgttggttaa
     1261 tgatttctac acattatcgt gcaccattaa atttaaacga agaagctttt acaacttcaa
     1321 agaaggaact agacaagatt gcggcagctt ataaacaagc atgcgtaaaa ttggcattat
     1381 caagtgctca agaagctact acatttgatg aaacgtatac ttcatttatg gatgcattaa
     1441 atgatgattt aaatacgcct aacgcaatta gtgtcgttta tgaagtggtt aagaaaatca
     1501 atcaggccgt acgtcaaaga gaaatcgatg ttgaaaattt agctgtttta gtacatacat
     1561 tagaaaagat gctttatatt ttaggtattg aatttgataa agtttcaatg actaacgaag
     1621 acaaagagat gtatcaaaac tggagaagtg ctgtaaaaga aaaagatttc aaaacagcgg
     1681 atacacttcg tcaaacattg attgaaaaag gaattctgta atggaaatag taacagaaaa
     1741 tgcgactagt cttgcatgga ttggtgatgc cgttatgtct ttggcagtac gtgaacacct
     1801 tttgagttta gggtttcgta aggcccatgt tcttcaaaag aaaaatgctg ttatttgtag
     1861 cgcaaaagga caagccgtca tgctaaataa aatgatggaa gaagattttt ttacagaaga
     1921 tgaaaagaca attttagcgc gtggaagaaa tgccactatt cattcaaaag ctaaaaatgc
     1981 gacacacaaa acatacttag aagcaaccgc attggaatca atgcttggct atttgtattt
     2041 atatggccat caagaaagac tgaaggtttg tcttgataaa attattgaaa taggggatag
     2101 tctatgatcg tatatggaaa aaacgtatat tctacacttc aaaatgatat ggattcaatt
     2161 gaaaaggtat atgtacttca aggattgaaa gatgcaaaac ttttgaagtc aatccaaaaa
     2221 tcaaatttaa aagtagaata ttgtaatcgt gcaaaattag ataagattgc caatactaca
     2281 catcataatg gtgtggcaat taaggttagt gaaattgaaa cgtatagtat tgagcagatt
     2341 gtatctcgcg caaaaaaacc aggtcttctt gttgccttgg atggaataca agatccacat
     2401 aatgtaggtg caattttaag aacttgtgat tgtgcaggtg tagatggtgt aattttaaca
     2461 aaacacaaca gttgtggttt aacgccaacg gttgtgaagg caagtacagg agcagcatat
     2521 acagtgcctg tttccattgt cacaaattta tcacaaacac ttagaaactt aaaacaagaa
     2581 ggatattggg ttgtcggaac cgatatgaaa gaggctaggg aatatcgtga aggtatgtat
     2641 gattcgccga ctgtattagt aattggttca gaagggcaag gcatttcaaa tcttgtaaaa
     2701 aaacaatgcg attatatggt ttacttaccg atggtaggaa gtatcacttc attaaatgcg
     2761 agtgtggcag ctgctatctt gatgtatgaa gtatataatc aaagaaatcc aaaaaaataa
     2821 gtcaattttt aaaaattggc ttttttaatg tctttttcac attgccttat tataatgaat
     2881 ttaggtgata gttatgaaca tatatgaaag tatatactta attcaaatga atgattttta
     2941 tgagatgaat catttaatcc aatattacag accaatggtt acagaaatat gggcggatat
     3001 atttgtgcgt aagacgaata caatattatg tgaaagagat gagttttaca gacatgcaga
     3061 cttattgttg tatcgatgta tttttgaata tcgtatggat cgtgtattaa catttacaag
     3121 tttttatagg cgttgcacca aaaataaagg gtatgatctt ttacgtcaag ccatacgcaa
     3181 acaatgttat ctaaatcagg tttgtataag ttttgatcaa aaagtgaaag aagatacgaa
     3241 cttgtattat agtggaatca gcacggatga ttctttagaa gtacatgaaa ttgtgatgga
     3301 taagttgatg tgggagcaca ttaagacaag aattatgaat ttatttggcg aggaatatgt
     3361 tcaaattgtc gatttacgta tggagggata tacctttaaa tatatagcgc aaaaacttgg
     3421 attaagctgt gcaaaggtta attttatgtt tgaaaaggta aaaaaatggt atacgacaat
     3481 tgacagttga atggtttaaa tggtacattt aatgagttaa ggagcgtgaa ggaattggca
     3541 gataaagttg ttttgacgtg tacagagtgt ctatcaagaa attatacgac cgaaaagaat
     3601 aagaagacca acacgcaacg tcttgaaatt aaaaagtatt gtccaaagtg tggcaaacat
     3661 actttgcata aggaaacaaa gtgaggaatc catatgagta aagaaaaaaa ggaaagaaca
     3721 tattcacctt caaaggtgtt caaagaatta aagactgtaa aatggccaac atttaaagaa
     3781 ttgatgtcct caagtgcttt agttattgta tttacagtat tatttggttt gtatttcttt
     3841 gtttgtgaga ttttatcaag tggtctagtt aaaatgatcg ttggcgcata gggaggaaat
     3901 tatgtcagag ttaaaaaaag aatggtatgt agtaaataca tatgtaggtc aagaaaatcg
     3961 tgtaaaagaa aatttagaac gtcgtattaa aactatgggt cttgaagact tcttgtttca
     4021 gatcgtcgtt gcggaagaaa aagaaattga atataaaaat ggtaaaccgg ttgaaaaaac
     4081 acataactta ttctctggtt atttgttagt gcaaatgatt atgacagatg aagcatggta
     4141 tattgtccgt aatacaccag gtgtaactgg atttatcgga tcaagtggaa agggtgcaaa
     4201 accattccca gtagctcaag aagaagtaga tgcggtattg cgtcgtttag gtcgtcaaga
     4261 tgtaaatgtt caggttgatt ttgaagtggg agatcgcgta gaaatcacaa atggtgcatt
     4321 caaaaattca gaaggtacta ttgaatctat ggatgaagaa aagaaagaag ctacagtatt
     4381 attgatttta tttggtcgtg aaacaccaac agaaattcca tatatggatt taaagaaagt
     4441 cgactaagac tttctttttt taggtatgtt tatgaagact ttagtatttg atttagatgg
     4501 cacaatgtat cgtggtaccc aaattattga aagtgctcat caatttttag attattgtat
     4561 ccaaaataag attccgttta tttttttgac aaacaattct atgcgtacac cacaagaaaa
     4621 tgtaaaacat atgcaagata tgggatatac aaacatagag ccatccatgt tttataatag
     4681 tgctatggca gcttgtcaat atgttaagaa acactacaag ggaaataggg catattatat
     4741 cggaaaacaa ggtatgaaag atgcgttgga tcaagaaggt tttattgttg atgatgaaaa
     4801 tccggatttt gtgtttattg gattggataa agatgcgaca tataaatctt attcaaaagc
     4861 gttatctttg attttaaatg gagctaagat cattggtaca aataatgatc gtattttggc
     4921 aaaacctggc ggatttgaag taggcaatgg aagtgtagtc gatatgtttg agtatgcagc
     4981 caatcaaaaa tcccctcgta ttggtaagcc caatcgagct attcttgatt tatgtttgga
     5041 gtattttggc ttaaaagaag atgaaatcat attgatcggt gacaatttag aaacggatat
     5101 taaacttgga tttgaacaaa atattgaaac agtacttgta cagacgggtg ttcataaaaa
     5161 ggaagatatt gaacaactaa agatttatcc gacgcacgta gtgaatacgt taatggattt
     5221 agtggactat aattttgact agtatttttt ttgaattatt ggtataatcg aaatatcgta
     5281 gaaggtgtga tgaaaaaatg aaagatatat taaacgtggt tttaatgaca ttatctggtg
     5341 ttattattat attaacatta ttacaaagtg gtaaatcaga cggattatcg gctgccttta
     5401 caggaagtgg agatttgaac ttgtttgcgg tacaaaaaga aagaggacca gaaaaagtta
     5461 tctctcgctt aactttgttt tgtgggattt tgttctttgc gattgtgatc gcattgcaga
     5521 ttgtgtaatt aaaagaccgt taaggtcttt tttctgtttt aaaggaggaa aaaatgaaag
     5581 aaaaaatcat tcaatattta gaagaaaaaa aagaatgtaa aatacgagat ttagaaaaaa
     5641 tagtaagacc taaatcaagt aaagaattta cagaaatggt gaagtcttta aatgagttag
     5701 aagatgaaaa gttgatttat aacgatcata caagtttgta tttgattgat gatatacatt
     5761 ttatggctgg aagcgcaaaa gatatttcga gatatgaaat agctgtcttt aatgaaaaca
     5821 ataaagtata tgttcaaaaa aataaaaaga tgaagatctt tgataaagat gaagtgttag
     5881 ttcgcttaaa gcctgaacca tctttaatta aagtgtttaa tcatggattg acaaatatta
     5941 caggagttat gattgttaaa aagaagaaga cattctttta tagtgatcta gacttacatt
     6001 gttcgattcg tgtagtcaat gaaaaagaat ttagttttaa aaaacgtaca aaagttgtat
     6061 gtcaaatcgt aaaatatgca gatccactag aagtaagaat tattcaggtt ctaggacatc
     6121 cagatgatgc aggtgttgat attagtgcga tgcttacaag taatcaagtg cgtcaggtgt
     6181 ttaatggaaa aattgtgaag gagtgcaaaa gaattcctgc taaagtacaa aaggatgaac
     6241 ttgaaaatcg taaagatcta agaggtttat taacggttac cattgatggg gatgatgcca
     6301 aggattttga tgatgcgatt tctattgtga aaaatgaaaa aggatattta ttgtatgtac
     6361 atattgcgga tgtttctcat tatgtaaaag aaaatagtgc ccttgatcaa gaagcgtatt
     6421 taagaggaaa ctcaatctat gtatgtgatc gtgtcgtacc tatgcttcca tttgatttaa
     6481 gcaatggtat ttgttcatta aatccaaatg tagatcgact cacaattact tgtgctatgc
     6541 aagtggatga aaagggaaat ctattgacat acaatatttt tccaagtgtg attcattctg
     6601 acaagcgttg tacatataaa aaagtgaatg cgtgtttaga tggtgataaa acaattttac
     6661 aagagtattc tgaagtaaaa gaattgttgt ttactttaga gtctttggct aaattattaa
     6721 agaaacagtc acataatcgt ggatgtattg attttaatac caaagaagca aagatcatta
     6781 tggataaaaa tggatatcca gtggatgtta aagtgttaga acgtggtttt gctgaacaaa
     6841 tgatcgaaga atgtatgatt ttagccaatg tttgcgtagc tcaccatatg catgtcaata
     6901 agataccaag tatgtatcgt gtgcatgaaa atccagatcc taaaaaggtg gcaacaattt
     6961 gtacggttgc gaattctttg cgtattccat ttgattttta tccagatgaa gtcaatgcga
     7021 gtcaaattca aaaattctta gaatccattc aagatgaaaa tgcatatgaa gtcttgtcta
     7081 tggtttcttt gcgttctatg cagaaggctc gctatgatgc acattgtctt ggacactttg
     7141 gacttgcttt agaagaatat tgtcatttca caagtccaat tcgtcgttat tcagatttag
     7201 tcgtacaccg tatgttgcat gcatattgtt ttaaagaaca aaagcgcaca ccatcaaatg
     7261 atgaaaagaa aatcgaaaaa caagccttcc atatttctga aaaagaaaga gatgcgatca
     7321 tgatggaaag aatggtcaat gattataaaa aggcacaata tatggaaaaa cgtgtaggtc
     7381 aagtttttga aggtacaatt gttggtgtaa tgagttttgg attctttgtg gaaatggata
     7441 atacggttga aggtcttgtt cctgttcata ctctttcaaa cgacttctat gattatgatg
     7501 aagatacaat gactttaaag ggacagacac atggtaaagt gttccaaatg ggcatgcgag
     7561 tacgtgtagt ttgtacggat gttcaaaggg aaaaaggaca agttacattt agcttgttag
     7621 aaagaagaaa tcatgattaa gggattcacc atgttgcatt tagaacaata ggatgaaatg
     7681 atttattttt atcattattt actagaacta cctatattaa gaaagtggga cacaggcatt
     7741 catttggatt gctttaatag ttgtattgaa atctttttgg atggtacaaa cagttgggga
     7801 aggatgtatt cgtcattttg cgtttgcaac agattatgtg gataaaattg tcgatagaga
     7861 gttcgttaaa gtgagtgttg agcctaaaga tgtagttatg aaaacagatc ctgtatttct
     7921 tattcgtata gattttattg atggtctgct tcatgaatct atcgaattgt tttgtgaaaa
     7981 ggcataatcg gtaggttttc tgtccaagtt ggtacttgat ttgcacataa ttatttcttt
     8041 gttagaatgg aatcagttaa acaaaggagt gattgaaatg aatcaagttc aagttgtaaa
     8101 attaaataaa aatttcaatc tttctttgta catatgtaaa ctggggtgaa ctatcattcc
     8161 agtttttctg tgatttcatg acaataaagg atgaggagga tgattgaaat ggatcagaat
     8221 tttaataagg tattttggga tgcatgcgca aatggcgatt ttccaatggt ttgttctcaa
     8281 atcaaagcag gagcagatgt aaattatcag aatggtgatg gacgtacagc tttgatgcgt
     8341 gcaagtaaac gcgatcgtaa agatgtggta caagttttac ttgataatgg ggcagatgtg
     8401 aatattacag ataataaagg gaaaacagct ttgatgacag ctgcaaaaaa aggcaataaa
     8461 acaatcttaa aagctttgat tgatgcaggt gcagatgtca atgccaaaga tgatagaggt
     8521 cgtacagctt tgatgcgtgc ttgtttatta ggacaagatc gttgtgttga agtgttatta
     8581 gatgctggag ctagaatcaa tgatcaagat gaagtaggtc gtagtgcttt aatggaagct
     8641 tgtatcgcat ttaaacgtga cacaattcac ttgttgcttg aaagaaatgc cgatgtgaat
     8701 ttatttgata ataatggtgt aactgcattg atgcgtgctg cttatggtgg atatccaagt
     8761 ttagttgaaa aattattggc atatggagct gataaagata tgacagataa acaaggtcgt
     8821 gttgcattgg attatgttcg tgaacattgt agagctcaat tagagcctat cttaagatga
     8881 ggttaagcgt atgagagatt atcaaagttt tgaagtacgt aacgtaagcg taatcggaca
     8941 tagtggagct ggaaaaacaa gtgtattaga agctatgttg tactatacaa aagcaataga
     9001 tcgctttgga aaaacaacag aaggaagttc aatgattgat tttgacactg aagaaattcg
     9061 tcgtggactt tctgtttata caacaatcgt acctattgaa tgggaagaca gtaaaatcaa
     9121 ttttattgat acaccaggtt atttagactt cgttggagaa aaacaatgtg gcttttcagt
     9181 tggtgattca agcttgattg tagtcaatgc caaagagcct ttacaatcag gaacaaaact
     9241 tgcgtataaa caagccgttg ctgaaaagaa acctacaatc ttcttcatca
//
LOCUS       DBMDWL010000075         9260 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2656_length_9260_cov_2.489791, whole genome shotgun sequence.
ACCESSION   DBMDWL010000075 DBMDWL010000000
VERSION     DBMDWL010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2656_length_9260_cov_2.489791"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="ACZPDN_06365"
     CDS             complement(<1..121)
                     /locus_tag="ACZPDN_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL35"
                     /protein_id="HIR1298281.1"
                     /translation="MPKMKSHRGLAKRVKKTGSGKLKRSHAYTSHRFHGKTKKQ"
     gene            complement(135..686)
                     /gene="infC"
                     /locus_tag="ACZPDN_06370"
     CDS             complement(135..686)
                     /gene="infC"
                     /locus_tag="ACZPDN_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1298282.1"
                     /translation="MVSNINNRKVAPNNVNDDLFNEKIPFREVLVINADGEQLGVMSK
                     KEALDIAYKQNLDLLCVAPKARPAVCKVLDYGRYHFEQQKKAKEAKRKQHVVEVKSLR
                     LSPIIDTHDFETKVRQTSKWIEAGMKVKIDMRFRGRLITRLEVGRKIMNDFIEAMSEV
                     AVVEKKPSMEGNTMSTVLAPKKK"
     gene            complement(915..990)
                     /locus_tag="ACZPDN_06375"
     tRNA            complement(915..990)
                     /locus_tag="ACZPDN_06375"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Lys,seq:ctt)
     gene            complement(1074..2936)
                     /locus_tag="ACZPDN_06380"
     CDS             complement(1074..2936)
                     /locus_tag="ACZPDN_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1298283.1"
                     /translation="MKLQLSNIQKSFGAKTVLAGANFQVRDNEKIALVGRNGCGKTTL
                     MKIICGQENYDSGSRMVLNGTNIGYLAQITFVDEEKTVYEELLTAFDKVRELEKQLNE
                     QAEVLKTDSSEKQLAKYDALQTRFEALNGYQYEVELKNVFFHFQFDENDLNKKLCEFS
                     SGQKTRIALVKLLLTKPDILLLDEPTNHLDIAAIEWLENYVKHYPFAVVLVSHDRMFL
                     DHVVDEIVEIEFGKTQRYVGDYSHYLKAKEEYLKKNHEAYVRQQQEIHRLEDLIEKFR
                     YKKNKAAFAKSKQKYLDRMDKIEDSSSDTSQMKAVFTCARKGGKQVLDVEDLSVGYDK
                     VLSKVSFQLLQGQRMAIVGPNGIGKSTLIKTLVKELPAISGSFKFGHQIDVGYFNQES
                     AQMKSNKNVLDELWDMDSDATQTQIRNTLASFLFTQDEVFKEVNDLSGGERVRLALAK
                     LMLEHDNVLLLDEPTNHLDIPAKEALESALEKYDGTILFVSHDRMFLKKMATRVLEMD
                     SVGKIYDLNYEEYLDKKANNELIESVVKVEKEVKPQTVSFTDLKAIKNRVAKLEVLLD
                     EAEQDLEAMRELRYEPEYYQDYRKMEELDAKIDDKHNEIAALMKEWEEKMAMME"
     gene            complement(2949..4235)
                     /locus_tag="ACZPDN_06385"
     CDS             complement(2949..4235)
                     /locus_tag="ACZPDN_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298284.1"
                     /translation="MKKTRVINKKNLSTMIIGMVTIAVAIVCVFLYVSKPKQTNKSSQ
                     SYLNGVDVDKLQKKVGKDFEGTYLLKDYVVYGETLSLYKSKYGSTETDKMQGNNIVLR
                     NVMTDATTSFTFNGSVDSGVDLGTLKEGIYELYTYNHFEKERIYFKDAFKAKAVTTMR
                     RDEKVTSVSFMADKNALKEYGIQFKKNYAFIIVTSQEPNEDIYDVVIDPCGNAMNYFS
                     NTVDVGASTSVLDEASSSLEFAKKVKKELEKKGLKVKILRKENETPGYYGANGRPARA
                     YKTKAKLYLALGASLDENVYAPYMLTSPYTNSGLANTVSYFMEQNGLTLYEARTNTTQ
                     ENGVLYDSYAESDNGKVLEYELYPQLRETGGKATYAGVYGDEFKNVDYKDAYGMYGLY
                     FSYASAMNSQSVTYYNENADAIAKILVKSIVRYFEI"
     gene            complement(4293..5648)
                     /gene="asnS"
                     /locus_tag="ACZPDN_06390"
     CDS             complement(4293..5648)
                     /gene="asnS"
                     /locus_tag="ACZPDN_06390"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR1298285.1"
                     /translation="MLVKKLKETFEQYDGQSVTLQGWVRTNRNSKAVGFIALNDGTTF
                     SNVQVVYGEKLENFDEVSKITTGSAMEVTGKLVCTPDAKQPFEIQAENICVLGLCDHD
                     YPLQKKRHSFEFMREIPHVRPRANTYYAMFRLRSVLSMAIHTFFQDRGFVYVNTPEIT
                     GNDAEGAGECFTVTTREDAKYDKDFFGKHAQLTVSGQLHVEPFALAYRNVYTFGPTFR
                     AENSNTTRHASEFWMIEPELAFADLADNMDCIEEMIKYCINYCLEHAKEEMEFFASMI
                     DKECIKRVTHVANSSFERMTYTKAIEYLEKAKDQFENQDIFWGMDLQSEHERYICEKV
                     VNGPVFLTDYPKDIKAFYMRINDDNKTVAACDLLVPYVGELVGGSQREERYDVLVNRM
                     KEMGMEQETLQWYLDLRRFGGCKHSGFGLGFDRMLMYLSGVANIRDVQPYPRTPRNLI
                     F"
     gene            complement(5885..6520)
                     /locus_tag="ACZPDN_06395"
     CDS             complement(5885..6520)
                     /locus_tag="ACZPDN_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR1298286.1"
                     /translation="MQNRNVPKATLQRYPVYLKALRKLQKNGVERIMSKELSSFVDIE
                     PTTIRRDFSFLGNLGKQGYGYDVDKLIEIFNSQLGVDFDEKIILVGAGNLGRALMNYN
                     KWNYVVGEIACAFDVDPKKCGTQFGVEVYPMSELENKIPEGCRIAILTISHDVQETVD
                     KLVQCGISGIVDFTHQHFLVPKGVVIKSIDVVSSIQELVFITNSLETKTQK"
     gene            complement(6603..7214)
                     /locus_tag="ACZPDN_06400"
     CDS             complement(6603..7214)
                     /locus_tag="ACZPDN_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298287.1"
                     /translation="MADHFLGALKEIERRAQNNILVFSDVLSERLDVIAQSMISKPLS
                     DNDYLKLAELYYKKFSKEKNKQGMLFCLLRMQQIVFLKEHTDKQTDDIKLEFSEEIDA
                     ITKAFLSRKRNYYQNSLRNIFQRFILLDTLAYVLLLMLFVLLFHIPFKVAFIILVLAW
                     IVVLVYAKQKGVPYFYDSRIQTLSQEVDSTFLQVDQGIFTKVE"
     gene            complement(7207..7455)
                     /locus_tag="ACZPDN_06405"
     CDS             complement(7207..7455)
                     /locus_tag="ACZPDN_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1298288.1"
                     /translation="MGKYSYTPKGVCSRQFNFEIEDGIIQSFEAIGGCNGNLKGIGCL
                     LKGRSVDEVIDTLTGVTCGNKPTSCPDQIACALKAYRG"
     gene            complement(7442..7951)
                     /locus_tag="ACZPDN_06410"
     CDS             complement(7442..7951)
                     /locus_tag="ACZPDN_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR1298289.1"
                     /translation="MNVKKSVILAFLVAVGILLQLLESFVGIFMIVPGYKIGLANIAG
                     LFALYIYDEKSLAYVSLLRIFLSSLMQGTLFSVSFYLSLSGGILACIAMIIAYRSKVF
                     SIYGVSILGACFHNIGQVIAITWIYQQYFMQLFLPILLALSIVSGILIAVVCKKVLSR
                     IHGGFYGKI"
     gene            complement(7948..8304)
                     /locus_tag="ACZPDN_06415"
     CDS             complement(7948..8304)
                     /locus_tag="ACZPDN_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR1298290.1"
                     /translation="MRKADKILIVCIMVLSIFLCIPLFFTKHVNSYASVYVKEKQVLR
                     IDLSKDKSYVVNGKLGKVHIEVKQKKVRVTQEKSLHHYCSKQGFVSDANVPIVCLPNE
                     TVVKIEKEASQDTVIQ"
     gene            complement(8301..8822)
                     /locus_tag="ACZPDN_06420"
     CDS             complement(8301..8822)
                     /locus_tag="ACZPDN_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298291.1"
                     /translation="MFKKIGVVVLSFLLCACSMGVQLKKQTFTIELGKDIYANPTLYL
                     KDSSRTSRLEVVSKSVGVDKKNNRFMTSGMDYLVVGEYDFAIQDGNKEYPFVIKVKDT
                     TPPVCASEVSQITISVGQNIDWNSYFHATDLSGVTFEANVDTSSASTQDVQVKISDRF
                     GNAVTKNVSVVVQ"
     gene            complement(8815..>9260)
                     /gene="whiA"
                     /locus_tag="ACZPDN_06425"
     CDS             complement(8815..>9260)
                     /gene="whiA"
                     /locus_tag="ACZPDN_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR1298292.1"
                     /translation="LPAKVIERKSVYVVYIKAGDKIADFLRLCNASNALFEFEDSRIQ
                     RDFYNQITRLDNCEVANEVKSLKAAKSQLEAIEFLESNAKNIVIPEKIMHAMQIRKMY
                     PEANLNELCLECKNEFGEDISKSGMKHRLAKVKQMAKDLKEDLDV"
BASE COUNT         2942 a         2026 c         1139 g         3153 t
ORIGIN      
        1 gttgtttctt agtctttcca tggaaacggt gagatgtata agcgtgagat ctctttaatt
       61 ttccagaacc tgtctttttt acacgttttg ctaatcctct atgactcttc attttaggca
      121 tagtctagtc ctccttactt ctttttaggt gccaacactg ttgacatcgt attaccttcc
      181 attgaaggtt tcttttcaac gacagctact tcagacatag cttcgatgaa gtcattcatg
      241 atcttacgac caacttccaa gcgagtgatc aaacgaccac ggaatcgcat atcaatcttt
      301 actttcatac ccgcttcgat ccattttgac gtctgacgta ctttagtttc aaaatcgtgt
      361 gtatcgataa tcggtgataa acgcaaagat ttaacttcaa caacatgttg tttacgtttc
      421 gcttctttag cttttttctg ttgttcgaaa tgatagcgac catagtctaa aactttacac
      481 acagccggac gcgctttggg tgcaacacac aacaaatcta aattttgttt gtatgcaatg
      541 tccaatgctt ctttttttga cataactccc aattgttctc catccgcatt gatgacaaga
      601 acttctctaa acggaatctt ttcgttaaac aaatcatcgt ttacattatt tggggcaact
      661 tttctgttat tgatattcga caccatcctt tttttaattt tcacaaaaag aaaatgggtg
      721 cattcaacac ccatcactct tttaaacgaa ttcaaactga attggccgtg aacctatgtc
      781 cctctagaca tcaggtgaga aggggtgctt ctatctttcc atttctttta cctattgata
      841 ataccataca catgtggtat atgcaatatt tttttcaaaa aaataaagcc gactcatcga
      901 cttatacaca taaatggtgg actcttgggg attcgaaccc cagacccact gattaagagt
      961 cagttgctct gccagctgag ctaagagtcc atttatcagt gcccatatat attaccataa
     1021 catatttttg attgcaactg ttttttaaaa aaataaggat gtctttgaca cccctattcc
     1081 atcatagcca ttttttcttc ccattccttc attaaggcgg ctatttcatt atgcttatca
     1141 tcgatttttg catccaactc ttccatctta cgataatctt gataatactc tggctcatat
     1201 cttaattctc gcatagcctc taaatcttgc tcagcctcat ctaataaaac ttcaagtttc
     1261 gcaacacgat ttttaatggc ctttaaatcc gtaaaagata ccgtttgtgg ttttacctct
     1321 ttttcaacct tcactaccga ttctatcaat tcattattcg cctttttatc caagtattct
     1381 tcataattca aatcatagat tttacctaca gaatccatct ctaatacgcg cgtagccatt
     1441 ttctttaaaa acatacgatc atgcgataca aaaagaatgg ttccatcata cttttctaat
     1501 gccgattcca atgcttcttt agctggtata tccaaatggt ttgtaggctc atctaaaagt
     1561 aaaacattat catgttctaa cattagtttc gcaagagcta aacgcactct ttcgccaccc
     1621 gacaaatcat tcacttcttt aaacacttca tcttgcgtaa acaaaaaact agccaacgta
     1681 ttacgaatct gcgtttgcgt tgcatccgaa tccatatccc ataattcatc aagaacattt
     1741 ttattagatt tcatctgggc actttcctga ttaaaatatc ccacatcgat ttgatgacca
     1801 aatttgaaac taccagaaat ggcaggaagt tctttcacca aagttttgat caaagtggat
     1861 ttaccaatac catttggtcc aacaatggcc atacgttgtc cttgcagtaa ttgaaaggac
     1921 actttagaca acactttatc atagccaacg cttaaatctt caacatcaag aacttgttta
     1981 ccaccctttc ttgcacaagt aaacaccgcc ttcatttgac ttgtatccga tgaactatct
     2041 tcaattttat ccatacgatc caaatatttc tgtttactct tcgcaaaagc ggctttgttc
     2101 tttttatatc tgaacttctc aatcaaatcc tctaaacgat gaatttcttg ttgttgacga
     2161 acatacgcct catgattctt ttttaaatat tcttcttttg cctttaaata atgcgaataa
     2221 tcccccacat atctttgtgt cttaccaaat tcaatttcta cgatttcatc cacaacatga
     2281 tccaagaaca tacgatcatg gctaaccaaa acaaccgcaa aaggataatg cttcacataa
     2341 ttttcaagcc attctatagc tgcgatatcc aaatggtttg taggttcatc caacaataaa
     2401 atatctggtt tcgtcaacaa taatttaact aaagcgattc ttgttttttg tcccgaacta
     2461 aattcacata attttttatt taaatcattc tcatcaaatt gaaaatgaaa aaatacattc
     2521 tttaattcca cctcatattg atatccattt aaagcttcaa aacgagtctg caacgcatca
     2581 tatttcgcaa gttgcttttc actcgaatct gtttttaaaa cttccgcctg ctcatttaat
     2641 tgtttttcta attcacgaac cttatcaaaa gctgttaaaa gctcttcgta taccgtcttt
     2701 tcctcatcca caaaagtaat ctgtgctaaa taaccaatgt ttgttccatt caataccatg
     2761 cgtgaaccag aatcataatt ttcctgccca caaataattt tcatcaacgt cgttttaccg
     2821 caaccattac gtccaactag cgctattttt tcattatcac gtacttgaaa atttgcacca
     2881 gccaaaactg ttttggcacc aaaagacttt tgaatattgc tcaattgtaa tttcatactt
     2941 tatcaccctt aaatttcaaa atatctaaca atacttttaa ccaatatttt agctatcgca
     3001 tccgcatttt cattatagta agtaacgctt tgcgaattca ttgcactcgc atatgaaaaa
     3061 taaaggccat acattccata tgcatcttta taatctacat ttttaaactc atccccatat
     3121 acacctgcat atgtagcttt tccacctgtt tctcttaatt gaggataaag ttcatactct
     3181 aaaacttttc cattatcact ttctgcatac gaatcgtata aaacaccatt ttcctgtgta
     3241 gtattcgttc ttgcttcata taaagtcaaa ccattttgtt ccataaaata tgaaactgta
     3301 ttggccaatc cactatttgt ataaggactc gttaacatgt atggagcata aacattctca
     3361 tctaaactag cacctaaagc taaatataat tttgcctttg tcttataagc acgtgctgga
     3421 cgaccattcg ctccataata gcctggagtt tcattttcct tacgcaagat ttttactttc
     3481 aagcctttct tttccaactc ttttttcact ttcttggcaa attctaaaga agagctagcc
     3541 tcatctaaaa cagatgtact tgcaccaaca tcaactgtat ttgaaaaata attcatcgca
     3601 tttccgcaag gatcaataac cacatcataa atatcttcat ttggttcttg acttgtcact
     3661 ataataaaag cgtaattttt cttaaattga attccatatt ctttcaatgc atttttatcc
     3721 gccataaaag atacggaagt caccttttca tcacgacgca tcgttgtaac agcttttgcc
     3781 ttaaaagcat ctttgaaata gattcgctct ttttcaaaat gattataagt atataattca
     3841 tagattcctt cttttaaagt tcctaaatca acacctgaat ctacgcttcc attaaaagta
     3901 aaactcgttg tcgcatccgt catgacattt cttaatacaa tgttgttacc ttgcatctta
     3961 tctgtttctg ttgatccata tttacttttg tacaaagata acgtttctcc ataaaccaca
     4021 taatccttta atagatacgt accttcaaaa tcctttccca cctttttttg taatttatca
     4081 acatccacac cattcaagta agattgagat gatttattcg tttgtttcgg ctttgaaaca
     4141 tataaaaaaa cacagacaat cgcaactgca atcgtaacca ttccgataat cattgtgctt
     4201 agattttttt tatttattac tcttgttttt ttcatacccc ttatttatat cacacaagaa
     4261 aacaggtatc aacgagatac ctgtttaaat cattagaaaa ttaaatttct tggtgtacga
     4321 ggatatggct gtacatcacg aatgttagct acaccactta aatacattaa catacgatca
     4381 aatcctaaac caaatccaga atgtttacat cctccaaaac gacgtaaatc taaataccat
     4441 tgtagagttt cttgttccat tcccatttct ttcatacgat ttactaatac atcataacgt
     4501 tcttcacgtt gtgatccacc aactaattct ccaacatatg gaactaacaa gtcacatgcc
     4561 gcaaccgttt tgttgtcatc atttatacgc atatagaaag ctttgatatc ctttggataa
     4621 tccgttaaga acaccggacc atttactact ttttcacaaa tataacgctc atgctcactt
     4681 tgtagatcca taccccagaa aatatcttgg ttttcaaatt gatctttcgc tttttctaaa
     4741 tattcgattg cctttgtata tgtcattctt tcaaaactag aattagccac atgcgttaca
     4801 cgcttaatac attctttatc aatcatagac gcaaagaatt ccatttcttc tttggcatgt
     4861 tctaaacaat agttgataca atacttgatc atttcctcaa tacaatccat gttatctgct
     4921 agatccgcaa atgcaagttc tggttcaatc atccagaatt cagaagcatg acgagttgta
     4981 tttgaatttt ctgcacggaa agtaggaccg aatgtataaa cattacgata tgctaaggca
     5041 aaaggttcaa catgcaattg tccagatact gttaattgtg catgttttcc aaagaaatcc
     5101 ttatcatatt ttgcatcttc acgcgttgta acggtaaaac attctccagc accttccgca
     5161 tcattaccag tgatttctgg tgtatttaca taaacaaatc cgcgatcctg gaagaatgta
     5221 tgaatagcca tacttaaaac acttcttaat ctaaacatcg cataatatgt atttgcacga
     5281 ggacgtacat gaggaatttc acgcataaat tcaaagctgt gacgtttctt ttgtaaagga
     5341 taatcatgat cacataatcc taaaacacaa atattttcag cttgaatttc aaatggctgt
     5401 tttgcatctg gagtacatac taatttacct gttacttcca ttgcagaacc agttgtaatt
     5461 ttagatacct catcaaaatt ttctaatttt tctccatata caacttggac atttgaaaaa
     5521 gttgttccat cattcaatgc aataaaacca actgcctttg aattacgatt tgtacgaacc
     5581 caaccctgca aagttacact ttgtccatcg tactgttcaa aagtttcttt taattttttt
     5641 actaacatag tttctccctt caaaataaaa aaaacgtccc ttaataatta aggaacgatt
     5701 ataaccgcgg taccatcctg tttcatcaca cgtatgtatg cactcaattg gaatttaacg
     5761 cattccacac gtcactgtct acttaatttc tccagtccga ctccacaggt gttatctatc
     5821 caaacttaca ttatcccttt tcaccaacta ggactctctt ctaaacgttt gattcggacg
     5881 acttctattt ctgcgtcttt gtttctaatg aatttgtaat aaataccaat tcttgtatag
     5941 atgataccac atcaatactt tttatgacaa ccccttttgg aaccaaaaaa tgctgatgcg
     6001 taaaatctac tatgccactg attccacact gaaccaattt atctaccgtt tcttgtacat
     6061 catgtgaaat tgttaaaatg gcaatacgac atccttctgg aatcttattc tccaattcag
     6121 acattggata tacttcaaca ccaaattggg ttccacattt tttcggatcc acatcaaagg
     6181 cacatgcaat ttcgccaact acataattcc acttattata attcattaat gcacgaccta
     6241 agttacctgc acctactaat ataatctttt catcaaaatc aacgccaagc tgactattaa
     6301 atatttcaat caacttatcc acatcatatc catagccttg ctttccaaga ttgcctaaaa
     6361 atgaaaaatc acgtcgaatc gtcgttggtt caatatcaac aaaactcgat aattccttag
     6421 acataattct ttcaacgcca tttttttgta gcttacgtaa agcttttaaa tacactggat
     6481 atctttgtaa agtcgctttg ggcacatttc tattctgcat ttatatcacc ccatggctta
     6541 ataataacac atttgtgata tatttaacaa gtataacaaa tgaacttgtg aaaaaaagtc
     6601 gattactcga cttttgtaaa aataccttga tccacttgta aaaatgttga atccacctct
     6661 tgtgaaagcg tttgaatacg cgaatcataa aaatatggaa cacccttctg ttttgcatag
     6721 acaagtacaa ctatccaagc aagcacaaga attataaatg caactttaaa aggaatgtgg
     6781 aataaaagca caaataacat caacaataaa acatatgcca atgtatccaa caaaataaat
     6841 ctttgaaata tatttcttaa tgaattctga taataatttc tttttcttga taaaaaagct
     6901 tttgtaatgg catcaatttc ttcactaaac tccaatttaa tgtcatctgt ttgtttatcc
     6961 gtatgctctt ttaaaaacac aatttgttgc atccttaaaa ggcaaaataa cattccctgt
     7021 ttattttttt ctttcgaaaa ttttttataa tacaattcag ccaactttaa ataatcatta
     7081 tccgaaagcg gtttagaaat catgctttgt gctataacat ccaagcgctc acttaaaaca
     7141 tccgaaaaca caagaatatt attttgtgca cgacgctcaa tctcttttaa agcccctaaa
     7201 aaatgatcag ccacgatacg cctttagcgc acacgcaatt tgatccggac atgacgttgg
     7261 tttattacca caagttacac ctgttaatgt atcaataact tcgtctacac ttcttccctt
     7321 caacaaacaa ccaatacctt tcaaattacc attgcatcct ccaatggctt caaaactttg
     7381 aataattcca tcttcaattt caaaattgaa ttgacgacta caaacacctt ttggtgtata
     7441 actatatttt cccataaaaa cctccatgaa ttcgacttaa cactttttta catactactg
     7501 caattaatat accactcaca atggataatg ccaaaagaat tggcaaaaaa agctgcataa
     7561 aatattgttg atagatccaa gtaatcgcaa tcacttgtcc aatattatga aaacaagctc
     7621 caagtatgct gactccatat atagaaaata ctttacttct ataggcaata atcatcgcaa
     7681 tacaagccaa aatcccacca cttaacgata gatagaatga aaccgaaaat aatgttcctt
     7741 gcatcaaact tgataaaaaa atgcgaagta aagaaacata agccaatgat ttttcatcat
     7801 atatatatag tgcaaacaac cctgcaatat tcgcaagacc aatcttatat cctggaacta
     7861 tcatgaagat acctacaaaa ctttccaata gctgcaacaa gattcccacc gcaactagaa
     7921 atgctagaat tacacttttt tttacattca ttgtatcacc gtatcctggc tggcttcttt
     7981 ttcaattttt accaccgttt catttggcaa acacacaatt ggaacattgg catccgaaac
     8041 aaaaccttgt tttgaacaat aatgatgcaa actcttttct tgagtgacac gaaccttttt
     8101 ttgtttgacc tcaatatgca ctttaccaag ctttccattc acaacataag atttatcctt
     8161 tgataaatcg atacgcaaaa cctgtttttc tttcacatag acactcgcat agctattcac
     8221 atgtttagta aaaaacaagg gaatacaaag aaaaatagat aaaaccataa tgcatactat
     8281 caatatttta tcagcttttc tcattgtaca accacactta cattttttgt gactgcattg
     8341 ccaaaacgat cactaatctt cacttgtaca tcttgtgtag aagccgatga cgtatccaca
     8401 tttgcttcaa aagtcacacc tgatagatct gtcgcatgaa agtatgaatt ccaatcaata
     8461 ttttgaccaa cacttatagt gatttgactg acttcgcttg cacaaacagg tggcgttgta
     8521 tcctttacct taatcacaaa cggatattct ttattcccgt cttgaatcgc aaaatcatat
     8581 tctccaacca ccagataatc catgcctgat gtcataaaac gattgttctt tttatctaca
     8641 ccaacacttt ttgaaacaac ttctaatcga cttgtacgag atgaatcctt taaatacaaa
     8701 gttggattgg cataaatatc tttaccaagt tcaattgtaa acgtttgttt ctttaactga
     8761 acccccattg aacatgcaca taataagaaa gataaaacta caacccctat ttttttaaac
     8821 atctaaatcc tccttgagat ctttagccat ctgcttcacc ttagccaaac gatgtttcat
     8881 acctgacttt gaaatatctt caccaaattc atttttacat tccaaacaca attcattcaa
     8941 atttgcttct ggatacatct ttcgaatttg catcgcatgc atgatctttt ctggaatcac
     9001 aatatttttt gcgttacttt ccaaaaattc aatcgcctct aattgtgatt ttgccgcttt
     9061 caagcttttc acttcattgg ccacttcaca attatctaaa cgcgtgatct gattgtaaaa
     9121 atctctttgt attctagaat cttcaaattc aaacaacgca ttcgaagcat tacataaacg
     9181 caagaagtct gcaattttat ctccagcttt tatatatacc acataaacgc tttttctttc
     9241 gatgactttt gccggcaaat
//
LOCUS       DBMDWL010000076         9181 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2680_length_9181_cov_2.554846, whole genome shotgun sequence.
ACCESSION   DBMDWL010000076 DBMDWL010000000
VERSION     DBMDWL010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2680_length_9181_cov_2.554846"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="ACZPDN_06430"
     CDS             <1..1201
                     /locus_tag="ACZPDN_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1298293.1"
                     /translation="MDMPHFLTALNTLMFKEDEHVSVCATNGVYFYYNPLKVIELFQK
                     NSIFLNRAYLHSILHCLYSHIWLRKNRIEFIWNVACDIVVEYTLDSMHKKSVSRILSY
                     ARKDVYHQIEKLDGLSCITVYEWLCTRDDIQNLYYEFVVDDHASWPKEKDEKTPQSSS
                     VQKKWQSVAKQTVFDHKQKGKENDDGDAFLVSRLQAKKSKYSFSQFLKRFSIMKEEMQ
                     INEDEFDLSYYTYGMSIYKNMPLIEPLETKEVKKIYEFVIALDTSYSIDENLAKRFIS
                     NTYSILSTSNMFYKSCKVYILQCDDRIRKENVISNQNEMNRLLDSFTLIGGGNTDFRP
                     VFSYVNDLVNQHVFQNLCGLLYFTDGKGVYPKKCPAYKTAFIYLEDYDQSKVPTWAIQ
                     YRLEEKL"
     gene            1198..2667
                     /locus_tag="ACZPDN_06435"
     CDS             1198..2667
                     /locus_tag="ACZPDN_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1298294.1"
                     /translation="MNILQAKNEIKNTVQAYLSKNSFGEYEIESKNQRPLLLIGPPGI
                     GKTQIMEQISKECQIGLVSYTITHHTRQSAVGLPFIQEKEFQGKTYSITEYTMSEIIA
                     SVYSKMEETGLKEGILFIDEINCVSETLAPTMLQFLQNKTFGNQKVPSGWIIVAAGNP
                     SEYNKSVRDFDVVTMDRIRYIHVETDYYVWKEYAISKHIHPMILSYLELKPNNFYTVS
                     TSVDGMEFVTARGWEDLSYLLYTYEKMHLEISQDTIYEYLHHEDIAEDVFAYYLVYKK
                     YQDDYGIDDILNGNVSSTIYARLFNADFDERVSVVNLVMDGLLRQVNIYSFEKHCTDL
                     WFGFLKNYRSHMDVSYVDHVKEFIALYHDKLQAELLSVAQKHEYEFVLNKIQKVNVDE
                     SLDSKSRFDCSTKPFFKQKENMESIESDLKLQIENAFEFMESTFKNGQEMTIFVTNLT
                     MSAEMALYLSSHTIGIYEKYKQVLLIGSQKARLLDELAH"
     gene            2681..3460
                     /locus_tag="ACZPDN_06440"
     CDS             2681..3460
                     /locus_tag="ACZPDN_06440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR1298295.1"
                     /translation="MDYSELKYDVEEFESDQDKKLPQPPLVKESMRKESIELPKNFDA
                     LQICSNFLDIINTRHSSRVYTQEPMSLLELSYILWTCQGVKQIRGKKYATLRTVPSGG
                     ARHGFELYFVCQNVEGLDPGTYHYLPLKHCIEFLNPIDKVSDVLASSLCDQLWALKAN
                     VILYFSYIPYRTEWRYGHLAHRIALVDLGHVGENIYLASTSIGLGTCGIGACVTSICD
                     EMFELDGENEFILYSQPVGKVNPEDFAKEKSFYEFVEKEGL"
     gene            3497..4777
                     /locus_tag="ACZPDN_06445"
     CDS             3497..4777
                     /locus_tag="ACZPDN_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298296.1"
                     /translation="MSKNDKNIDAYSKALLDKKTEYNEELKKPKEVHQMTEAERRLAE
                     KTMSSALDMLRKERGQKTIAEEERLYEENKVDSQHMEDDFTTSSIENEQSSDALDLVH
                     TMLQDMSNDDDELLPSQVKKLKEKQEKKEKKEKKEKPKKEEGPKEKKLKKKKPMSKVT
                     KIILGCILLGVAALGVYSYKVLVYDPQNIVSPAQQKTYDKLVSYADEYGDNMMSDAEK
                     LELIDLNSGYKKLLDKQKTSINAYFKEQTGKSYTALLKEVKELQKTKEEVKLPAYQQI
                     VDLLNGWDAKSDEEKMAVADMQSVYSSLSKSLKKEVDSLSKSQTGKDFIDLCKEQAEL
                     KTAAQEKAQQEEEQKKSENASKIQEYQASLNSLQQDYDTYAQYAQSLESELADASDED
                     RSQIQSQIDTNNQMLYSLQQQIAYYQQQIAALQS"
     gene            4810..4884
                     /locus_tag="ACZPDN_06450"
     tRNA            4810..4884
                     /locus_tag="ACZPDN_06450"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4842..4844,aa:Thr,seq:cgt)
     gene            4936..6069
                     /locus_tag="ACZPDN_06455"
     CDS             4936..6069
                     /locus_tag="ACZPDN_06455"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR1298297.1"
                     /translation="MSLDEKMILLATCFLFLPYQIASVGIACILVQAFCRHKLVDAIK
                     NQTGAIFLYGFIGLEFIVTILYSNWNGFGNTWLFVLIGFYGAYYRKSITKSTFEKMCD
                     LIIILSIFAAIYGLYQFNQISIANGRTFLEFHIFNSPKRRITSTFMNANIYAMVIDFV
                     CVMCMYRFVKNDNILCKIGYVIVAFFNFFVLYLTGSRTALLPFVLIFPIFLYCVRWKK
                     LFLTSIVLELCVCGLVFLKPTLIPRMSDMSTFASRIKIWKTAFICISMYPLFGWGPQT
                     YKKFYPLVHGHKAPHAHNIYIDSILSYGVIGTILVLCYTFVLNKEIFTSNTRKENPAL
                     FGMMMCFIAIVLIYGLLDCTLNIVATGTLCFIILNSACMYKEK"
     gene            6109..7296
                     /locus_tag="ACZPDN_06460"
     CDS             6109..7296
                     /locus_tag="ACZPDN_06460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR1298298.1"
                     /translation="MKYISLFVCFFIGLQTLNVPDYERLISNIESKVEIVDESKASFL
                     SLKCRDVTNAIQIEKANLICRIEIQSQKKDKLESKYVQKKNYYELKKLEYGLDLNAIQ
                     KQDCMIQSYWIDKDLTKCFNEYKCSQNQYLKSRKQWKKNKHKLDLCESYLTRLDNCQK
                     RLFSRFSSLSQFNDLGEFDQDAMIAGNDALPAYGGYEILENKGMSCLVDGNYAIDTYS
                     SFWQNPVENGTISARCWSYPDGSLHLGLDIASSMYSDVYALANGIVLYAHAPVESNNG
                     YLGNMCGWPSGAGNSICMVVAVHDKLYAVSYAHLSNEIYVTSGQQVSQKTVIAKSGNS
                     GNSTGPHTHIEVFELKQDLNSTVEYFRNSGADFSFGCGFNAAATCSDYACRIDPEIVL
                     EGL"
     gene            7293..8600
                     /locus_tag="ACZPDN_06465"
     CDS             7293..8600
                     /locus_tag="ACZPDN_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIR1298299.1"
                     /translation="MSQEMYFSRMKELLKDEYDAYMESLSKPLYRGMRINLKKAELNE
                     FDHLESPSQFCKESYYVDKPLGNHPYHICGCFYLQEPSASSAVEILDVQEDDYVLDLC
                     AAPGGKSTQIASHLKSGYLVSNEIDAKRAQILLSNIERMGVKNVAVTNSSVDKLAKVF
                     DCCFDKILVDAPCSGEGMIKKHDIALNHWSIENIELCAARQKEILEYAYSMLKPGGTL
                     VYSTCTYALEEDEDVVLSFLEKHEDIQLVDPEVLWGRKGFKDLNVRRIFPMDGGEGHF
                     IAKFKKQSGIAGKLPVLKSKKIDKITEKFLKEQLNTLPNYYYIDNDRVYGMDVCFVDF
                     KKVKVLRQGVYLGDVIKNRFEPSHSFYMVADFDYKRKVEVTLEEMDLFMHGEVLQKEC
                     EKGYVAVCYKGHPFGFGKSDGRQIKNKIPKGLRLLPNSHVNID"
     gene            complement(8616..>9181)
                     /locus_tag="ACZPDN_06470"
     CDS             complement(8616..>9181)
                     /locus_tag="ACZPDN_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR1298300.1"
                     /translation="NLILGMNLDIEYIMPAHGIIWKDYIGDIIETYKGIAAQKTENKA
                     VIVYESVWKHTQQIAESLAEGFSDAGMDVKVYKLSDTKSSIVMREIMDAKVVCIGSGT
                     YNNVMAPSVAAFLEHIRPCKLKNKKGLAFAAYGWFNQVAKEIDTRMQQSGIESCHDVI
                     NQCYTPSDDQLEDYYNIAKEIAKNCQE"
BASE COUNT         3141 a         1182 c         1863 g         2995 t
ORIGIN      
        1 tatggatatg ccacattttt taacagcgtt aaatacgttg atgtttaaag aggatgagca
       61 tgtaagtgta tgtgctacaa atggagttta tttttattat aatccattga aggtgattga
      121 gctttttcaa aagaattcga tctttttaaa tcgtgcttac ttacatagta tcttgcattg
      181 tttatatagt catatttggc taagaaaaaa tcgtatagaa tttatttgga atgtagcttg
      241 tgatattgtg gtggaatata cattggattc aatgcataaa aaaagtgttt cacgaatctt
      301 atcctatgct cgaaaagatg tatatcacca aattgaaaag ttggatggat tgagttgtat
      361 tacggtttat gaatggcttt gtacacggga tgatattcaa aatttatatt atgaatttgt
      421 ggtggatgat catgcatcat ggcctaaaga aaaggatgaa aaaacacctc aatcgtcttc
      481 ggtacaaaag aagtggcaaa gtgttgccaa acagacagta tttgatcata aacaaaaagg
      541 taaagagaat gacgatgggg atgcttttct tgtatcgagg ttacaggcaa aaaaatcgaa
      601 gtattcattt tcacaatttt tgaaacgttt tagtattatg aaagaagaaa tgcagatcaa
      661 tgaggatgaa ttcgatttgt cgtattacac atatggtatg tctatttata aaaatatgcc
      721 tttgattgag ccgttagaga caaaagaagt caagaaaatc tatgagtttg tgattgcgtt
      781 ggatacaagt tattcgattg atgaaaatct tgcgaagcgt tttatatcaa atacatatag
      841 tattttaagc acttcaaata tgttttataa atcatgtaag gtatatatcc ttcaatgtga
      901 tgacagaatt cgaaaagaga atgtcatttc caatcaaaat gaaatgaatc gtttgttgga
      961 ttcatttact ctaatagggg gaggtaacac cgattttagg ccggtgttta gctatgtgaa
     1021 tgatttggtg aatcaacatg tatttcaaaa tctttgtggt ttactttatt ttacagatgg
     1081 aaagggcgtt tatccaaaaa agtgtccagc ttataaaacg gcattcatct atttagaaga
     1141 ttatgatcaa agtaaagtgc ctacttgggc aattcagtat agattggaag aaaaattatg
     1201 aatatattac aggcaaaaaa tgaaataaaa aatacagtac aggcatattt atcgaagaat
     1261 agctttggtg aatatgaaat tgaatctaag aatcaaaggc ctttattgtt gattggtcct
     1321 ccgggtattg gtaaaacgca gattatggaa caaatatcga aggaatgtca gattggactt
     1381 gtcagctata cgattacgca tcatacgcgt caaagtgcgg tgggacttcc ttttatccaa
     1441 gaaaaagaat ttcaaggcaa aacctattca attacagaat atacaatgag tgaaattatt
     1501 gcgagtgtct attcgaaaat ggaagaaacg ggtttaaaag aaggaattct atttattgat
     1561 gaaatcaatt gtgtatcgga aacattggct cctacaatgc ttcaattttt acagaataaa
     1621 acctttggaa atcagaaggt cccaagtgga tggatcattg tggctgcagg aaatccgagt
     1681 gaatataata aatcagttcg tgattttgat gttgtgacaa tggatcgtat tcgttatatt
     1741 catgtagaaa cggattatta tgtttggaaa gaatatgcga tctcaaaaca tattcatcca
     1801 atgattttaa gctatctaga attaaagcct aacaattttt atactgtttc aacaagtgta
     1861 gatggtatgg aatttgtgac ggctagaggc tgggaagatt tatcgtattt attatataca
     1921 tatgaaaaga tgcatttgga aatatcgcaa gatacaatct atgaatattt acatcatgaa
     1981 gatattgcag aagatgtttt cgcctattac ttggtctata aaaaatatca ggatgattat
     2041 ggcattgatg atattttaaa tggaaatgta tcaagtacga tctacgctcg tttgtttaat
     2101 gcggattttg atgaaagagt atctgtagtc aatttagtga tggatggttt attaagacaa
     2161 gtgaatatat attcttttga aaaacattgt acagatttat ggtttggttt tctaaaaaat
     2221 taccgaagtc atatggatgt atcttatgtg gatcatgtaa aggaatttat agctttgtat
     2281 catgataagt tgcaggctga attattaagt gttgctcaaa aacatgaata tgagtttgtg
     2341 ttaaataaga tacaaaaggt gaatgtggat gaaagcctag attctaagtc gcgttttgat
     2401 tgtagtacaa aaccattctt taaacaaaaa gaaaatatgg agtcgattga atcggatttg
     2461 aaacttcaaa tagaaaatgc gtttgaattt atggaaagta catttaagaa tggtcaggaa
     2521 atgacaatat ttgtcacaaa tttaacaatg tcagctgaaa tggctttgta tttaagttca
     2581 catacaattg gtatatatga aaaatacaaa caggttttat tgattggaag tcaaaaggca
     2641 agattattag atgagttagc tcattaaaga aaggaattat atggattatt cagaattaaa
     2701 atatgatgtg gaagaatttg aatcggatca agataaaaaa cttccacaac cacctttggt
     2761 aaaagagtca atgcgaaagg aatccattga acttcctaaa aattttgatg cgctacaaat
     2821 ctgttcaaac tttctagata ttatcaacac acgtcatagt agtcgtgtgt atacgcaaga
     2881 gccaatgtcc ttattagaac tatcttatat tctttggact tgtcaaggtg tgaaacaaat
     2941 tcgtggaaaa aaatatgcga ctttgcgtac agtaccttct ggaggagcac gtcatggttt
     3001 tgaactgtat ttcgtatgtc aaaatgtaga aggattagat cctggaacct atcattattt
     3061 acctctaaaa cattgtattg agtttttgaa tcctattgat aaagtgtcag atgttttggc
     3121 atcttcctta tgtgatcaat tatgggcttt aaaagccaat gtaattttgt attttagcta
     3181 cataccatac cgaacggaat ggcgttacgg tcatcttgca catcgaatcg cattggtgga
     3241 tcttggtcat gtgggtgaga atatatattt ggcaagtaca agtattggct taggaacttg
     3301 tgggattgga gcttgtgtga cttcgatttg tgatgaaatg tttgaattgg atggcgaaaa
     3361 cgagtttatt ctttactcgc agccagttgg gaaggtgaat ccagaggatt ttgcgaaaga
     3421 aaaatctttt tatgaatttg ttgaaaaaga aggcttataa tgtgttaaaa tagattggaa
     3481 ctaaggaaaa tatattatga gtaaaaatga taagaatata gatgcttatt caaaagcact
     3541 tttagataaa aaaacagaat ataacgagga actgaaaaaa ccgaaagagg ttcatcagat
     3601 gacagaagct gaaagaagac ttgcagaaaa gacaatgtct tctgctttag atatgcttcg
     3661 aaaagagcgt ggacaaaaaa ctattgcgga agaagagcgt ctttatgaag aaaataaagt
     3721 tgactcccaa catatggagg atgattttac aacttcatct attgaaaatg agcagtcgag
     3781 tgatgcatta gatcttgtac atacgatgtt gcaagatatg tcaaatgatg atgatgaatt
     3841 attgccttcg caagtaaaga aattaaaaga aaaacaagaa aagaaggaaa aaaaggaaaa
     3901 gaaagaaaag ccaaagaaag aagaaggacc aaaagaaaag aaactgaaga aaaagaaacc
     3961 tatgagtaag gtcacaaaga ttattttagg atgtatctta ttaggtgtgg cagctttagg
     4021 tgtatattca tataaggtat tggtttatga tccacaaaat attgtttcac cagcacaaca
     4081 gaaaacatat gataagttag tatcctatgc ggatgaatat ggagataata tgatgtcgga
     4141 tgctgaaaag ctagaactta ttgacttgaa ttctggttat aaaaagcttt tagataaaca
     4201 aaaaacgtca atcaatgcat attttaaaga acaaacaggt aagtcttata cggctttatt
     4261 aaaggaagta aaagaacttc aaaagacaaa agaagaagtg aaacttccag cttaccaaca
     4321 gattgttgat ttgttgaatg gatgggatgc aaaatctgat gaagaaaaga tggctgttgc
     4381 cgatatgcag tcggtgtata gttctttatc aaagagcttg aaaaaggaag tggattcttt
     4441 atcgaaatca caaacgggaa aagattttat tgatttatgt aaagaacaag ctgaattaaa
     4501 aacggcagct caagaaaagg cgcaacaaga agaggaacaa aagaagtctg agaatgcttc
     4561 aaaaatccaa gaatatcaag ctagtttaaa tagtttgcag caagattatg atacatatgc
     4621 gcaatatgcg caaagtttgg aatcggaatt ggcggatgct agtgatgagg atcgttcaca
     4681 gattcaatcg caaattgata caaacaatca aatgttgtat tctttgcagc aacagatcgc
     4741 atattatcag caacaaattg ctgctttaca atcttagaaa aaaaggctat ccttatgggt
     4801 agtcgatatg ccgacttagc acagtggtag tgcaacgcac tcgtaacgcg taggtcattg
     4861 gttcaaatcc gatagtcggc accatgattt aaaatcaagc tggtttatcc agcttctttt
     4921 tttgaaggag tatctatgag tttagatgaa aaaatgatat tgttggcgac atgctttctt
     4981 tttttacctt atcaaatagc cagtgttgga attgcatgca tattagtgca ggctttttgt
     5041 cgacataaat tagtggatgc cataaaaaat caaacaggag ctattttttt atatggtttt
     5101 attggtttag aattcattgt tacgatttta tattcaaact ggaatggttt tggcaataca
     5161 tggctatttg tgttgattgg attctatgga gcttattatc gaaaaagcat cacaaagagt
     5221 acttttgaaa agatgtgtga tctaattatt attctatcta tttttgcagc tatttatgga
     5281 ctttatcaat ttaatcagat ttcaattgcg aatggaagaa ccttcttaga attccatatc
     5341 tttaattcac caaagcgaag aattacatcg acatttatga atgcgaacat ttatgcgatg
     5401 gtcattgatt ttgtatgcgt gatgtgcatg tatcgttttg tgaaaaatga caacattctt
     5461 tgtaagattg gctatgttat tgtggcattc tttaacttct ttgtgttata tctaacggga
     5521 tctagaacag ctctcttacc ttttgtactg attttcccta tctttttata ttgtgttcgt
     5581 tggaagaaat tatttcttac atctattgta ttggagttgt gtgtatgtgg cttggtgttt
     5641 ttaaagccaa cgttgatccc tagaatgagt gatatgtcaa cattcgcatc tcgtattaag
     5701 atttggaaaa cggcatttat atgtatttca atgtatccac tttttggatg gggaccacag
     5761 acatataaaa agttttatcc tttggtgcat ggtcataagg caccgcatgc gcataatatt
     5821 tatatagatt ctattctttc ttatggcgta atcggaacaa ttcttgtgtt atgttacact
     5881 tttgtattga ataaagaaat ctttacttcc aatacaagaa aagaaaatcc agctttattt
     5941 ggtatgatga tgtgttttat cgcaatcgta ttgatttatg gattgttaga ttgcacactc
     6001 aatattgttg cgacaggaac tttatgtttt attattttaa atagtgcttg catgtacaaa
     6061 gaaaaataaa tacaatgaat ttagaagaca gacagtagaa ggagttttat gaaatatata
     6121 tctctttttg tctgtttttt tattggtctt caaacactaa atgtgccaga ttatgaaagg
     6181 ttgatttcaa atatagaatc aaaagtagaa atagttgatg aaagtaaggc tagtttttta
     6241 tctttgaaat gtagagatgt aacaaatgcg attcaaattg aaaaggcgaa tctaatttgt
     6301 agaatagaga ttcaaagtca aaaaaaagat aaacttgaat caaaatatgt acagaaaaaa
     6361 aactattatg aactcaaaaa actggagtat ggtttagact taaatgcgat tcaaaaacaa
     6421 gattgtatga ttcaatcgta ttggatagat aaggacttaa cgaaatgttt caatgaatat
     6481 aagtgtagtc aaaatcaata tttgaaaagt agaaaacaat ggaaaaagaa taagcataag
     6541 cttgatttat gtgaatctta tttaactcgt ttagacaatt gtcaaaagcg tttgttttcg
     6601 cgcttttcga gtttgagtca attcaatgat ttgggagagt ttgatcaaga tgcaatgatt
     6661 gctggaaatg atgcattacc tgcatatggc ggatatgaaa ttttagaaaa caagggtatg
     6721 tcttgtttag ttgatggaaa ttatgcgatt gatacatata gttctttttg gcaaaatccg
     6781 gttgaaaatg gtacaatcag tgctagatgc tggtcatatc cagatggaag tcttcatttg
     6841 ggtttggata tcgcaagtag tatgtatagt gatgtatatg ccctagccaa tggcatagta
     6901 ttgtatgcgc atgcacctgt agaatctaat aatggatatc ttggaaatat gtgtggatgg
     6961 ccaagtggag ccggcaattc gatttgtatg gtggtagctg ttcatgataa gttatatgcg
     7021 gttagttatg cgcacttgtc taatgaaatc tatgtaacaa gtggccagca agtcagtcaa
     7081 aaaacagtta tagcgaaaag tggaaatagt ggaaattcaa caggtcctca tacacatata
     7141 gaggtgtttg agttaaaaca ggatttgaat tcgactgtag aatattttag aaacagtggt
     7201 gctgattttt cttttggatg tggttttaat gcggctgcaa catgctcaga ctatgcatgt
     7261 cgtatagatc ctgagatagt tttggagggt ttatgagtca agaaatgtat ttttcgcgaa
     7321 tgaaggaatt attaaaagat gaatatgatg catatatgga atctttgtca aaacctttat
     7381 atcgtggtat gcgtattaat ttaaaaaagg cagaattaaa tgaatttgat catttagaaa
     7441 gtccaagtca attttgtaag gaaagctatt atgtagataa acccttggga aatcatcctt
     7501 accatatttg tggatgtttc tatctacaag aaccaagtgc atcgagtgca gtcgaaatct
     7561 tggatgtgca agaagatgat tatgtattgg atttatgtgc agctcctggt ggaaagagta
     7621 cgcagattgc ttctcattta aaaagtgggt atttagtatc gaatgaaatt gatgcgaaaa
     7681 gagcacagat tcttttatcc aatatagagc gtatgggggt taaaaatgta gctgtaacca
     7741 attcgagtgt agataagctt gcgaaagtat ttgattgttg tttcgataag atattagtag
     7801 atgctccatg tagtggtgag ggtatgatta aaaagcatga tattgccttg aatcattgga
     7861 gtattgaaaa tattgagctt tgtgcagctc gccaaaaaga aatcttagaa tatgcgtatt
     7921 cgatgttgaa accgggtggt actttggtat attcaacttg tacatatgct ttggaagaag
     7981 atgaagatgt cgttttatct ttcttagaaa aacatgaaga tattcaactt gtagatcctg
     8041 aagttttgtg gggaagaaaa ggatttaaag atttaaatgt gcgtcgtatc tttccaatgg
     8101 atggtggtga aggtcatttc attgcgaaat tcaagaaaca atccggtatt gcaggtaaac
     8161 ttccagtttt aaaatctaaa aaaatagata agattactga gaaatttttg aaggaacaat
     8221 tgaatacatt accaaactat tactatatag ataatgatcg agtctatggt atggatgttt
     8281 gttttgtgga ttttaaaaaa gtaaaagtat tacgtcaagg cgtatattta ggagatgtta
     8341 ttaagaatcg ttttgagcca agtcattctt tttatatggt tgcagatttt gactataaaa
     8401 gaaaagtaga agtaacgcta gaagagatgg atctttttat gcatggagaa gttttacaga
     8461 aggaatgtga aaaaggttac gttgccgtat gttacaaagg acatccgttt ggctttggca
     8521 agagtgatgg aaggcaaatc aaaaacaaga ttcctaaagg acttcgttta ttgccaaatt
     8581 cacatgtgaa tatagactaa aaaggccagg aaaatttatt cctggcaatt ttttgcgatt
     8641 tcttttgcaa tattataata gtcttctaat tgatcatcac ttggtgtgta acattgattg
     8701 atgacatcgt gacaagattc aattccagat tgttgcattc ttgtatcgat ttcttttgcg
     8761 acttggttaa accatccgta tgcggcaaag gccaatcctt ttttgttttt caatttacat
     8821 ggacgaatgt gttcaagaaa agctgcaaca cttggtgcca tgacattgtt gtaggttcca
     8881 gatccaatac atacgacttt agcatccatg atttcgcgca tgacaatgct tgattttgta
     8941 tccgataatt tatagacttt tacatccata ccagcatcac taaatccttc agctaagctt
     9001 tcagcaatct gttgtgtatg tttccataca gattcatata caataacggc tttgttttct
     9061 gttttttgag ctgcgatacc tttataagtt tcaatgatat caccaatata gtctttccag
     9121 ataataccat gggcaggcat aatatattca atgtctaaat tcatacccag aataaggttt
     9181 a
//
LOCUS       DBMDWL010000077         9145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2703_length_9145_cov_2.969483, whole genome shotgun sequence.
ACCESSION   DBMDWL010000077 DBMDWL010000000
VERSION     DBMDWL010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_9145_cov_2.969483"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            130..651
                     /locus_tag="ACZPDN_06475"
     CDS             130..651
                     /locus_tag="ACZPDN_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1298301.1"
                     /translation="MNKALAHLHTINKHKVKVTYLCFRCHLYKQGFLHDLSKYSYIEL
                     KTGFHYYQGFRSPIDAEKEEKGYSLGWLHHKGRNKHHWEYWLDFGVNGIYACKMPTNY
                     VIEMFCDRVAASMIYQKEKYTDSSALEYYENGRGKILIHPETDALIHHLLKYLSEHGL
                     DQTIHYIVTEIKE"
     gene            complement(652..2457)
                     /gene="glmS"
                     /locus_tag="ACZPDN_06480"
     CDS             complement(652..2457)
                     /gene="glmS"
                     /locus_tag="ACZPDN_06480"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1298302.1"
                     /translation="MCGITAYAGKENALPFLLQGLEKLEYRGYDSAGVTLVSSHHLET
                     YKAKGRLTNLEEILSEKEHPEHTGIGHTRWATHGVPSNMNSHPHTNESETISIVHNGI
                     IENYASIKECLLEQGYKFQSQTDSEVIVHMLDYYYQGDMMYALKKCVQYLQGSFALCV
                     VCTDYPDCVFVAKKESPMIVGKTDTAAFCASDIPAVLDYTKDICALEDDQMAVLKPGS
                     IELYDFFGDKVSMKWTHISYDACAAQKGGYDTFMQKEMHEQPYVIKETLRAHIENNLA
                     MPELSGLDVSKINQIYFVACGTAYHASLYAQYLLRTWIDLPIYCISASEFRYGNYRIN
                     ENTLCIFVSQSGETADTLAALKLSKENKAQTLAVTNVLGSSLARLSDFVLYTCAGPEI
                     AVASTKAYTTQLVLLACLCLKLAALCNGVLENQNHMIDQLKQMPEVVEEMLQQEDKMA
                     EFAKLLTNQKDAYFIGRQLDYLSVLEGALKLKEVSYVHADAYMAGELKHGPIALIEKD
                     TVVIALATQPNVAQKTISNILETVARGAKVILITSKNQNAEGFDYVIRIPDVDPLFSC
                     IPATVLLQELAYYVAKEKGCDVDKPRNLAKSVTVE"
     gene            complement(2698..3576)
                     /locus_tag="ACZPDN_06485"
     CDS             complement(2698..3576)
                     /locus_tag="ACZPDN_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1298303.1"
                     /translation="MDNKNNARLGKGLSSIFGQDVSKVLDDIQNGDMEVESQQQSKIP
                     VNEIRPNPYQPRKVFNDEALKELSSSIKQHGVFTPILVKKSIQGYDLIAGERRLRASK
                     LAGLENIPAIIVDFDDQEMMEIALLENIQREDLNVIEEAKAYEKLIQRLGYTQEQLAH
                     RVGKSREHITNLLRLLKLPEDVQEYVVNKQLSMGHVRALLGLKTESSMRKIAKQAIDQ
                     GLSVRKVEQIVKDTNNKKTVEKPKEDIYVKAAKEKLQEFFQTSVSISKNSISIHYENK
                     EDLNRILELLNLVEEE"
     gene            complement(3580..4356)
                     /locus_tag="ACZPDN_06490"
     CDS             complement(3580..4356)
                     /locus_tag="ACZPDN_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1298304.1"
                     /translation="MGKVMAISNQKGGVGKTTTSINLASGLAHVGKKVLLIDFDSQGN
                     ATQGLGANQNNSQATIYSVLMEGVPIQQAIVPKVNPRIDIVPANINLAGADLDMDKME
                     YGKEELLKKAIAPIRDQYDYIIIDCPPSLGLLNTNALTAADSILIPVQCEYYALEGVT
                     QLLITIRLVQRTSNRNLKIEGILLTMFDIRTRLSVDVSQDVRQTFGKLVYQNSIPRNV
                     KLSEAPSRGVSIFEYDPKSTGAKAYAGLTEEVLKRNSKEA"
     gene            complement(4359..5060)
                     /gene="rsmG"
                     /locus_tag="ACZPDN_06495"
     CDS             complement(4359..5060)
                     /gene="rsmG"
                     /locus_tag="ACZPDN_06495"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1298305.1"
                     /translation="MTKVEFQKACLEAGIQLNEEQMNQFEKYMQLLIEWNEKMNLTAI
                     TQEEEVWEKHFYDSIMPFSNIDFETMCDIGSGAGFPGIPVKIAFPHVHMTLVEPLQKR
                     CRFLNEVKTQLNLENLDILNVRAEDLAKEKRECFDVVSARAVAKLSILLELCVPFVKM
                     NRTMVALKGKNGHEELLNAKKAIDTLGVELVDEEKFMIEEDATRINLYFKKVKHTPSK
                     YPRAYGQIKKKPLEG"
     assembly_gap    5191..5200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5213..5800
                     /locus_tag="ACZPDN_06500"
     CDS             5213..5800
                     /locus_tag="ACZPDN_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR1298306.1"
                     /translation="MKNFKKILFILICILIAFIGGMFFMNKQTEPEITSTLIQNRIEQ
                     ASDLVTTKYHYTKVGKFENSLNLNGWSIPLTNKYFILTFEGEIQLGTDLSKANVEIND
                     STIHVTVDKPAVISNSIDESSIEVYDETKNIFNPISVSDYKAFALEQKDKALSEAKEK
                     GLMKTAQENTKKSIKEIVSIIPDTDDYTIEVTFKE"
     gene            5803..6447
                     /locus_tag="ACZPDN_06505"
     CDS             5803..6447
                     /locus_tag="ACZPDN_06505"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1298307.1"
                     /translation="MPNIKLVDAFTDFVEESTLPYRQMMTYYNCALMEVETKFKVLNE
                     QFSLFYDRNPIESIHTRIKSQSSILKKLQKKNLALTIPNIEKNLFDIAGVRVVCSFTE
                     DIYSLANSFLNQDDVRLIEKKDYIQNPKENGYRSLHLIVEIPIFLSDEKKWVKVEVQF
                     RTIAMDFWASLEHKIRYKKNLSKERLNEIDKELLECAKICKTLDEKMEEIKTGN"
     gene            complement(6456..7649)
                     /locus_tag="ACZPDN_06510"
     CDS             complement(6456..7649)
                     /locus_tag="ACZPDN_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvI family membrane protein"
                     /protein_id="HIR1298308.1"
                     /translation="MKKNENTKLNRSWMAAFSVATTAFAAHAGGGFATGNQENTWFVS
                     LGWPAIVGVAVAMLLLAMTIREGQIMMNSRGLKTYKELFECLYHPFDKVELLFELFFN
                     IMVLMVVASCISGAASALTQYFGLNYYLGTFVIGLVVLFLTIFGVDLIRRASRYMGIV
                     ILVLAVSIYTIGLLNGHNLLDVMRVDFSVHGWSQLPKAVWNGVTYSAFQWVTVPAILV
                     CGTSVLKTKQDCDRSMALTFTLNMLGLGLAVLMLLCWQHYYLTQPNGTTLPTLTTLKS
                     FGANWLVALYGLVLFLCLISSAVCVIFGFVNRFENVKFLQKVENVPVRRALVSAFIMV
                     VSMGISFVGLTNVVKYGYGYCGYLGIAIIIVPLLTVGFYKNRKFMKENSQDAKVSFEE
                     VYEEN"
     misc_binding    complement(7710..7950)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8032..9096)
                     /locus_tag="ACZPDN_06515"
     CDS             complement(8032..9096)
                     /locus_tag="ACZPDN_06515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HIR1298309.1"
                     /translation="MGLSKIILNIALKIVDILTFYIRPKKNHITFISLTQSELSSDFK
                     LIDKQLRHENKYDIHYILLMFEKNLWGDFKYFLNCLKQLVEIKKSSLVILNDNNYVIS
                     HMKPKNVKVLQIWHACGAVKKFGNQIKRQYPVQNYDYVLCNAEYWKDPYSQAFNVEKD
                     QVLVTGMPRIDTLLNLNGKDEFYLKYPELKDKKLCLYAPTFRGNIIDGFKIQSFDFTK
                     IKDYIVLYKFHPLLGDIQCKGGINMNKEDLYTLMQVSDCLISDYSSVIFDYSLLNKPM
                     ISYIPDIDEYKQDIGLNIDFNDFPGPVCTDEDQLMEALKFEDYDYDKLSYFQMKYMVY
                     TDGRNTSRVVDTINQIMEEA"
BASE COUNT         3026 a         1751 c         1243 g         3115 t
ORIGIN      
        1 aaaataaacc aataatcata tctctctttt aaaaataata ataaacaaaa aggaaccatt
       61 ctagacacca acactagaat ggttttctta ttattcatat gataaaatag gcaagaaaga
      121 agtgaattta tgaataaagc attagcccat ttacatacaa tcaataagca caaagtaaaa
      181 gttacatact tgtgttttcg ctgtcattta tacaaacaag gattcctaca tgacttaagc
      241 aaatactctt atatcgaatt aaaaactggt tttcattatt atcaaggatt ccgctctcct
      301 attgatgcag aaaaagaaga aaaaggctat tcattaggtt ggttacatca taaaggaaga
      361 aacaaacatc attgggaata ttggcttgat tttggcgtaa atggtatata tgcatgtaaa
      421 atgccaacca attatgtcat tgaaatgttt tgtgatcgag ttgctgcaag tatgatctat
      481 caaaaagaaa aatacaccga ttcaagcgca cttgaatatt atgaaaatgg acgaggtaaa
      541 attctgattc acccggaaac agacgccctc atccatcact tattaaaata tttatctgag
      601 catggtttag atcaaaccat tcattatatt gttacagaaa tcaaagaata attattctac
      661 agtcacactt ttcgcaaggt tacgaggttt atctacatcg caaccttttt cttttgcgac
      721 ataatatgcg agttcctgca ataaaacagt tgctggaata caactaaata gtggatccac
      781 atccggaata cgaattacat aatcaaaacc ttctgcattc tgattttttg aagtaattag
      841 aataactttg gccccacgag ccactgtttc taaaatattt gaaattgtct tttgtgcaac
      901 attaggctgt gtagctaacg caatcactac cgtatctttt tcaatcaatg cgataggacc
      961 atgttttaat tctccagcca tatatgcatc ggcatgtaca taagaaactt cttttagttt
     1021 taacgcccct tctaaaacac ttaaataatc caattgtcta ccaataaagt aagcatcttt
     1081 ttgatttgta agtagttttg cgaattcagc cattttatct tcttgttgaa gcatttcttc
     1141 taccacttct ggcatttgtt tcaactgatc aatcatatga ttttggtttt ctaaaacccc
     1201 attacataat gctgcaagtt ttaaacataa acatgccaac agcacaagtt gtgttgtata
     1261 agccttcgta cttgcgactg caatttctgg cccagcacaa gtatataata caaaatcaga
     1321 taaacgagct agactagagc ctaatacatt tgtcacagct aatgtttgtg ctttgttttc
     1381 ttttgaaagt tttaatgccg ccaaagtatc ggctgtttct ccagattgtg aaacaaagat
     1441 gcataaagtg ttttcattaa ttcgataatt tccatatcta aactcacttg cactaatgca
     1501 gtaaattggt aaatcaatcc atgtgcgcaa taagtattga gcatacaaac tcgcatgata
     1561 tgcagttccg catgcaacaa agtagatttg attaattttt gaaacatcta aaccagataa
     1621 ttctggcata gccaaattat tttcaatatg agctcttagc gtttctttaa tgacatatgg
     1681 ctgttcatgc atctcttttt gcataaacgt atcatatcca cctttttgag ctgcacacgc
     1741 atcatatgaa atatgtgtcc atttcataga tactttgtct ccaaagaaat catacaattc
     1801 aatactgcct ggtttcaaaa cagccatttg atcatcttct aaagcacaaa tatcctttgt
     1861 gtaatctaat acagctggaa tatcacttgc acaaaaagca gccgtatcgg tttttccaac
     1921 aatcattgga ctttcttttt ttgcgacaaa tacgcagtct ggataatctg tacaaaccac
     1981 acataacgca aaacttcctt gaagatattg tacacatttc tttaaggcat acatcatatc
     2041 tccttgatag taataatcca acatatgaac aatgacttca ctatctgtct gtgattgaaa
     2101 tttatatcct tgttccaata agcattcttt gatggatgca tagttttcga tgattccatt
     2161 atgtacaatt gaaattgtct cactctcatt tgtatgtgga tgtgaattca tattacttgg
     2221 aacaccatgt gttgcccaac gtgtatgtcc aattcctgta tgttcaggat gttctttttc
     2281 ggataatatt tcctctaaat ttgttaagcg tcccttggcc ttatacgttt ctaaatgatg
     2341 acttgaaact aatgtcactc ctgcagagtc atatccacga tactccagtt tttctaaccc
     2401 ctgcaataaa aatggtaaag cattttcttt acccgcgtaa gctgtgattc cacacattat
     2461 aaaaaaatcc tcctattata ttattttcat ggaggatact aaatggcttt gttcaatgtt
     2521 cacacattgg ttttgtccat cgttaaaggt tgtatcaacc tccagactac ccgccgaatc
     2581 tttcgataag tctggtcctc gtcaactagt ttctagtcct ggcgctattt ttttactaca
     2641 actccatgaa taatgaatat tatacaaaaa ataatacata tgtcaaaaaa agaagactta
     2701 ttcttcttca actagattca ataattctag tattcgattt aagtcttctt tattttcata
     2761 atgaatagaa attgaatttt tactgataga tacagatgtt tggaagaatt cttgtagctt
     2821 ttctttagct gctttcacat aaatatcttc ctttggtttt tctaccgttt ttttattatt
     2881 tgtatccttt acaatttgtt ctactttacg cacagacaac ccttgatcaa tggcctgctt
     2941 agcaatctta cgcatgctag attccgtttt aagacccaac aaagcacgaa catgccccat
     3001 acttaattgt ttatttacaa catattcctg tacatcttca ggaagtttta acaatcgcaa
     3061 taagtttgtg atatgttcac gtgattttcc tacacgatga gccaattgtt cttgtgtata
     3121 tcctaaacgc tgaatcaatt tttcataagc ttttgcttct tcaattacgt ttaaatcttc
     3181 ccgttgaata ttttctaaca acgcaatttc catcatttcc tgatcatcaa aatcaacaat
     3241 gatagctgga atattctcta atccagcaag ttttgaagct ctaagtcttc tttcacctgc
     3301 aatcaaatca tatccctgaa tacttttttt aactagaata ggcgtaaata caccatgctg
     3361 cttaatagat gaacttaatt cttttagcgc ttcatcatta aatactttac gaggctgata
     3421 tggatttgga cgaatttcat ttacaggaat ctttgattgt tgctgacttt caacttccat
     3481 atctccattt tgaatatcat ctaatacctt ggaaacatct tgcccaaaaa tactagacaa
     3541 gccttttcct aaacgagcat tatttttgtt atccataaat tatgcctcct ttgaattacg
     3601 ttttagtact tcttcagtta aaccagcata tgccttggca cctgtacttt ttggatcata
     3661 ttcaaaaatc gatacaccac gacttggtgc ttcagacaat tttacatttc ttggaattga
     3721 attttgatat acaagctttc caaatgtttg acgaacatct tgcgatacat caacactcaa
     3781 tcttgtacga atatcaaaca tcgttaataa aattccttcg attttcaaat tacgattact
     3841 tgttctttgt acaagacgaa ttgtaatcaa caattgcgtt acaccttcca atgcataata
     3901 ttcacattgt actggaatca aaatactgtc agctgccgtt aatgcgtttg tatttaataa
     3961 gccaagtgaa ggtggacaat caataataat ataatcatat tgatctcgaa tgggtgcaat
     4021 tgcctttttc aacaattctt ccttgccata ttccatttta tccatatcca aatcagctcc
     4081 agctaaatta atgttagccg gtacaatatc aattctagga ttcaccttag gcacgatagc
     4141 ctgctgtatt ggaacacctt ccattaaaac tgaataaatt gtcgcctgtg aattattttg
     4201 attcgcaccc aatccctgtg tagcatttcc ctgtgaatcg aaatcaatca ataatacttt
     4261 tttacctaca tgtgcaagtc cactcgccaa atttatagaa gttgtcgttt ttccaactcc
     4321 acctttttga ttagaaatcg ccattacttt tcccatactt aaccctccaa aggttttttc
     4381 ttgatttgcc catacgccct aggatattta ctaggtgtat gttttacctt tttaaaatat
     4441 aaattaatac gtgttgcatc ctcttcaatc atgaattttt cttcatcaac caattcaaca
     4501 cctaatgtat caattgcctt tttcgcattt aataattctt catgtccatt ttttcctttt
     4561 agtgccacca ttgtacgatt cattttcaca aaaggaacac aaagttctaa taaaattgat
     4621 aatttcgcaa cagcacgtgc gctgaccaca tcaaaacatt cacgcttttc tttcgctaaa
     4681 tcttcggctc gaacatttaa tatatctaga ttttctaagt tcaattgagt tttcacttca
     4741 tttaaaaaac gacaacgctt ttgtaaaggc tccaccaatg tcatatgtac atgtggaaat
     4801 gcaattttta ctgggattcc aggaaaacct gctcctgaac caatatcaca cattgtttca
     4861 aaatcaatat ttgaaaaagg catgattgaa tcgtagaaat gtttctccca cacttcttct
     4921 tcctgcgtaa ttgccgttaa attcatcttc tcattccatt caataagaag ttgcatatat
     4981 ttttcaaatt gattcatttg ttcttcattt aattggatgc cagcttctaa acaagccttt
     5041 tgaaattcta ctttagtcat gaaaaccctc cacgctaatc attatatcat ataatcgttt
     5101 ccgtcgtatt cttactatac gcaataattc gacattatac atgtcagttt attagctttg
     5161 ttcatttatg ttacaatttt aaaaaggagg nnnnnnnnnn aggaggtcac ttatgaaaaa
     5221 ctttaaaaaa attttattca ttcttatatg tatcttaatt gcctttattg gtggtatgtt
     5281 ctttatgaat aaacaaacag aaccagaaat cacatctaca ttaattcaga atcgaattga
     5341 acaggcgagt gatttagtaa ccacaaaata tcattatacg aaagtaggaa aattcgaaaa
     5401 ttcattaaat ctcaatggat ggtctatccc tttgacaaac aaatacttca ttctaacatt
     5461 tgaaggggaa attcaattag gtacagattt atctaaggca aatgtagaaa tcaacgactc
     5521 aacgattcat gtgactgtcg ataaacctgc tgttatttca aattcaattg atgaatcaag
     5581 cattgaagtc tatgatgaaa caaaaaatat ttttaaccca attagtgtga gtgactataa
     5641 agcttttgcg ctcgaacaaa aagataaggc tttatctgag gcaaaagaaa aaggattaat
     5701 gaaaacagct caagaaaaca caaagaaaag tattaaagaa attgtttcca ttattcctga
     5761 cacagatgat tatacaattg aagtcacatt taaggagtaa ttatgccaaa tataaaatta
     5821 gtcgatgcat tcacagattt tgtagaagaa agtactcttc catatcgtca aatgatgaca
     5881 tactacaatt gtgccttaat ggaagtggaa actaaattta aagtacttaa cgaacaattc
     5941 tccctattct atgatcgtaa tcccattgaa tccattcata cgcgtattaa atcgcaatct
     6001 agcattctta aaaaacttca aaaaaagaat cttgctttaa caatcccaaa cattgaaaag
     6061 aatctgttcg atattgccgg tgttcgagtt gtatgctctt ttacagaaga tatttattct
     6121 ttggccaact cttttcttaa ccaagatgat gtgcgcttga ttgaaaaaaa ggattacatt
     6181 caaaatccaa aagaaaatgg gtatcgaagt cttcatttaa ttgtagaaat tcctatcttt
     6241 ttatccgatg agaaaaaatg ggtcaaggta gaggtacaat tcagaaccat tgcgatggat
     6301 ttctgggcaa gtctagaaca caaaattcgc tataagaaga acttatcaaa agaacgctta
     6361 aatgaaattg ataaagaatt attagaatgc gctaaaattt gtaagacact tgatgaaaaa
     6421 atggaagaaa taaagacggg aaattaattc cccgtctaat tttcttcata tacttcttca
     6481 aaagatactt ttgcgtcttg agaattttct ttcatgaatt tacgattctt atagaatcca
     6541 actgtcaaca atggaacgat gatgatggcg attcctaaat atccacaata tccatatcca
     6601 tacttcacaa catttgtaag tcctacaaat gagattccca tagaaacgac catgatgaat
     6661 gctgaaacta aagctctgcg tactggtaca ttttcaactt tttgaagaaa cttcacattt
     6721 tcgaaacgat tcacaaagcc aaagatgacg cagactgcac ttgaaatcaa gcataagaat
     6781 aataccaatc cataaagtgc tacaagccag tttgctccaa atgattttaa tgttgtcaac
     6841 gttggtaaag ttgttccgtt tggctgcgtc aaataatagt gttgccaaca aagtaacatt
     6901 aatacggcaa gtcctaatcc taacatattc aatgtgaatg ttaatgccat tgaacgatca
     6961 caatcttgtt ttgtttttaa tacacttgtt ccacaaacta ggatagctgg tactgtcacc
     7021 cattgaaaag ctgaataagt aacaccgttc caaactgctt ttggaagttg actccatcca
     7081 tgtactgaaa aatcaacgcg cataacatct aataagttat gtccatttaa taagccaatt
     7141 gtataaatag atacagctaa tactaaaatg acaatgccca tatatctact tgctctacga
     7201 attaagtcga ctccaaaaat cgttaaaaac aatacaacaa gtccaatcac aaatgttcct
     7261 agatagtaat tcaatccaaa gtattgtgtt aatgcactgg ctgctccaga aatacaggat
     7321 gcgacaacca ttaaaaccat gatgttaaag aataattcaa ataatagttc taccttatca
     7381 aatggatgat ataaacattc aaataattct ttatacgttt ttaatcctcg agaattcatc
     7441 ataatttgac cttcacgaat tgtcattgct aaaagtaaca ttgcaaccgc tacgccaaca
     7501 atagctggcc aacctaaact gacaaaccat gtgttttcct gatttcctgt cgcaaatcct
     7561 ccgcctgcat gagccgcaaa agcagtagtt gccactgaaa atgctgccat ccaggaacga
     7621 ttcaattttg tgttttcatt tttcttcatt ctcaaattct ccttttctgc aacaaaaaac
     7681 cttccgtctc ttgttgctta ataaacaact tcagagacga aaggtaatat ttttattctt
     7741 ccgcggtacc actctaattc attctaacga atgccctcat cgatgcaatc acatctagtc
     7801 tttatatcgt aagactcacg tctataacta cattatcgct atagccactc catggtgatt
     7861 ttcattctct agctttacga tattttccac caaacaatat ctctcttggc caaaactaag
     7921 tgaattactc ttccatatca atgtgttgtc tgtataatac tacaatttac attttgtttc
     7981 aatattaaat tgtaattttt tcattaattt gtaaataaaa agaaagagga cctatgcctc
     8041 ttccataatc tgattgattg tatcaacgac tcgacttgta tttcttccat ctgtatatac
     8101 catgtacttc atttgaaaat aagataactt atcataatca taatcttcaa atttcaatgc
     8161 ttccatcaat tgatcttcat ccgtacatac tgggcctgga aaatcgttaa aatcaatatt
     8221 caatcctata tcttgtttat attcatcaat atctggaata tagctaatca tcggtttatt
     8281 taataaagaa taatcaaata tcacactcga ataatcggat attaaacaat cactcacctg
     8341 catcagtgta tataaatctt ctttattcat attgattcca cctttgcact gaatgtctcc
     8401 aagtaatgga tgaaacttat ataatacaat ataatcttta atttttgtga aatcaaaaga
     8461 ctggatttta aagccatcaa tgatatttcc tctaaaagta ggtgcgtata aacataattt
     8521 tttatccttt aattcaggat atttcaaata aaattcatct tttccattca aatttaacaa
     8581 tgtatcaatt ctaggcattc ccgtcaccaa aacttgatct ttttctacat taaaagcttg
     8641 agaataagga tctttccaat attccgcatt acataagaca taatcatagt tttgtacagg
     8701 atattgcctt ttaatttgat ttccaaattt cttcacggct ccacacgcat gccatatttg
     8761 tagtacctta acattctttg gtttcatatg actaatcaca taattattat catttaaaat
     8821 cactaagctt gattttttga tttcaacaag ttgttttaaa caattcaaaa agtatttaaa
     8881 atcaccccat aaattctttt cgaacatcaa taaaatataa tgaatgtcat atttattctc
     8941 atgacgtaat tgtttatcaa ttaatttaaa atcagacgat aattcacttt gagttaaact
     9001 aataaaagta atgtggttct tctttggacg aatataaaaa gttaaaatat ctacaatttt
     9061 taaagcaata tttaagatta tcttacttaa tcccatttta gtgcaccgcc ttgtatagaa
     9121 tatctacaac tcttttagat gcctt
//
LOCUS       DBMDWL010000078         9116 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2722_length_9116_cov_3.440710, whole genome shotgun sequence.
ACCESSION   DBMDWL010000078 DBMDWL010000000
VERSION     DBMDWL010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2722_length_9116_cov_3.440710"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="ACZPDN_06520"
     CDS             <1..302
                     /locus_tag="ACZPDN_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIR1298310.1"
                     /translation="DIGNLKNASLLYGVDVDEDLMCGKGHIFASHLKETVPGKYREIP
                     FGTGHTEFVKNIKTLKRLGVRMFVGEFWYVNNEDWQDVVKNAGVFLRDKLDKGFE"
     gene            303..1190
                     /locus_tag="ACZPDN_06525"
     CDS             303..1190
                     /locus_tag="ACZPDN_06525"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1298311.1"
                     /translation="MNIKAIVMDMDGTLLNSKDKISPKTKDALIECQKNGIKIVLASG
                     RNYGRIKPYLEELHLEEFGGLLIEINGIAIYDMSTNQRTILRQMHEDEVHPVFSYLMN
                     LNAESIAVYDEGLFDYMPDHILTMKQKLRLSLDLPEDYPWTAGAWSWFWDMRNGYPCQ
                     KYIEKVEEIDCPINKFQILQETDILNEIEEKLIHKYGDRFEVFRTTPRQIEMGPKGYT
                     KGDTLVRLMKNFGWSKDEVFVFGDGGNDISMFKQTNYSFAMGQALESVKNEAKYITLS
                     NDEDGIYEALMKFNVIKGV"
     gene            1192..2010
                     /locus_tag="ACZPDN_06530"
     CDS             1192..2010
                     /locus_tag="ACZPDN_06530"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1298312.1"
                     /translation="MENKILELYANRIRKHILTEVYSAKSGHPGGSLSAADILTELYF
                     EQMDINKDNVNTIDRDRFVLSKGHASPVLYATLAEKGFVDIEDLKGFRHIDSKLQGHP
                     NMNYVSGVDMSTGSLGQGISCAVGMAIANKVDENDHRIYCLLGDGECEEGEVWEAAMA
                     ASHYKLDNLCAILDYNHLQIDGNIDDVISPEPFKSKFEAFGWRVIEANGHDFNSLRTA
                     FNFAKQTVDKPTIIIAHTIKGKGVSFMENNYAWHGAAPNQEQYEQAMKELGGLE"
     gene            2010..2930
                     /locus_tag="ACZPDN_06535"
     CDS             2010..2930
                     /locus_tag="ACZPDN_06535"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1298313.1"
                     /translation="MATATREAYGKTLAKLIVEHDDLFVLDADLTKSTKTIDAKKARP
                     EHHFNMGIAEGNMMAVAAGMAASNKVVYASSFAMFAAGRAFEQIRNSICYPNLNVKVC
                     ATHAGISVGEDGASHQCIEDLALMRSIPNMKVFQPCDAKQTEEIIKAVYSIEGPCYVR
                     LGRLGVEDVYDENYKYEFGKGNVLKKGSKVAIIASGLMVQESLKAYDLLENKPTIIDI
                     PTIKPIDKELIIETAKTHDVIVTVEEHNVYGGLGSAVSEVLAPEGIACRQYMLGMQDT
                     FGRSGKPKELLDYYGLDAKHIAEKIKEIVK"
     gene            3045..3242
                     /locus_tag="ACZPDN_06540"
     CDS             3045..3242
                     /locus_tag="ACZPDN_06540"
                     /inference="COORDINATES: protein motif:HMM:NF027249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1298314.1"
                     /translation="MDYAMKVYPMHLENGKTEWCVEYPDLKGCVGGGDTIEEAIADAE
                     ATKAVYLNYLKENEKEQCSDS"
     gene            complement(3255..3578)
                     /locus_tag="ACZPDN_06545"
     CDS             complement(3255..3578)
                     /locus_tag="ACZPDN_06545"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR1298315.1"
                     /translation="MFRLFKKKEKIKRELPPGTIRKEFHFEGNVQNIGFRFEVQSHAK
                     PLGITGYAKNNDDGSVTAQLQGSLENIDKVILSLQNIDRIQIDSLTEKEIPLDYYEND
                     FYILY"
     gene            complement(3872..4015)
                     /locus_tag="ACZPDN_06550"
     CDS             complement(3872..4015)
                     /locus_tag="ACZPDN_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298316.1"
                     /translation="MNEVDVIKVDPKNTSKIGKEKYTKIKGLSVHYCAAYVINRRGMG
                     FVD"
     gene            complement(4079..5200)
                     /locus_tag="ACZPDN_06555"
     CDS             complement(4079..5200)
                     /locus_tag="ACZPDN_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298317.1"
                     /translation="MKVFSMSQRIYYKDLEPDAELIIKKDLELYNCILHKAFKICFDR
                     AYKDATYSETDQRMIKSFYGTNDYFPLSAIYEAKALVKSLKCREKEECDLIKTRIKKI
                     NKKIKKNEKQLKKALKEKEKLINQSKKKKYTEEDYLYEVQVLDPKIKRLKSIIHNLKF
                     RRNRNEFKLKRKMPSVCFGGKKNLKNGDLETYRFKRSRRLLITGRRQGKYSNNLFKLN
                     VDTDMLTYRSTQKDIVFKVQFHKYKDELYARVNEKHNSPDKAVAYELMDYGEYFIVKA
                     IFEKHMNLPKTDTLYGAIGIDINLDHIALCETNKDGNIVLIKKYPIHKENTKNKRNEE
                     LYQLAIEIMEQCKSKKKSLVVEDLNFKQLKTRMLYRPKK"
     gene            5558..5992
                     /locus_tag="ACZPDN_06560"
                     /pseudo
     CDS             5558..5992
                     /locus_tag="ACZPDN_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025677.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(6510..6767)
                     /locus_tag="ACZPDN_06565"
     CDS             complement(6510..6767)
                     /locus_tag="ACZPDN_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1298318.1"
                     /translation="MLYDRKDYYFSNKYKMQIVDRVGGGDSFGAGLVATSLEGYDAKS
                     IIEFSVAASCLKHSIEGDFNIVSMDEVLQLAGGDGSGRVQR"
     gene            complement(7646..7966)
                     /locus_tag="ACZPDN_06570"
     CDS             complement(7646..7966)
                     /locus_tag="ACZPDN_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298319.1"
                     /translation="MKAFLPNELDVKDKIQEIAGEDDEPINISIKLCLYCMKAQIFLN
                     GNKRASVIFANHYLISHGGGFLVIPEKEVPEFKRLLVKYYEGEDIAVITAFMKERCWK
                     TIES"
     gene            complement(7944..8525)
                     /locus_tag="ACZPDN_06575"
     CDS             complement(7944..8525)
                     /locus_tag="ACZPDN_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298320.1"
                     /translation="MPVQYSEIQELLRSRADLHARLNLMPYDGTPEIKERGAKKYLYV
                     RKRVAGKQTSTYVGAYTEELYNLLLRNAREIREIRKSLRSIEKQLAEAGYSEDELSIN
                     VVNNIAFAHANMKMNIYDQAVLEGVATSFPQTEEIIENGKVSGITATDVQKILNLKHA
                     WEFILDRDVVASRSDYYMLSHIARIVNEGFFAE"
BASE COUNT         2970 a         1478 c         1590 g         3078 t
ORIGIN      
        1 cagatattgg aaatttaaag aatgcatcct tattgtatgg tgtagacgtg gacgaagatt
       61 tgatgtgtgg aaaaggacat atttttgcga gtcatttaaa agaaactgtt ccaggtaaat
      121 atcgtgaaat tccatttggg acagggcata cagagtttgt gaaaaatatc aaaacattga
      181 aacgattagg tgtacgtatg tttgtcggag aattttggta tgtgaacaat gaagactggc
      241 aagatgttgt aaagaatgca ggtgtgttct taagagataa gttagataag ggatttgagt
      301 aaatgaatat aaaagccatt gtaatggata tggatggaac tttattgaat tcgaaggata
      361 aaatttcacc gaaaacgaag gatgctttaa tagaatgtca aaaaaatgga attaagattg
      421 ttctagcatc aggcagaaat tatggacgaa ttaaaccata tttggaagag ttgcatcttg
      481 aagaatttgg tgggttactt atcgaaatca acggtattgc gatatatgat atgtcaacga
      541 atcagagaac aatactaaga caaatgcatg aagatgaagt gcacccagta ttttcgtatt
      601 tgatgaattt aaatgcagaa agcattgccg tatatgatga aggtttattt gattatatgc
      661 ctgatcatat attaacaatg aagcagaagt tacgtttatc attagattta ccagaagatt
      721 atccgtggac tgcaggcgct tggtcatggt tttgggatat gagaaatggt tatccttgcc
      781 aaaaatatat tgaaaaagta gaagaaattg attgtccaat taataaattt caaattttac
      841 aagaaacaga tattttaaat gaaatagagg aaaaattaat tcataaatat ggggatcgtt
      901 ttgaagtttt tagaacaaca cctagacaga ttgaaatggg tcctaaaggc tatacaaaag
      961 gtgatacatt agttcgattg atgaaaaatt ttgggtggtc aaaagatgaa gtgtttgtgt
     1021 ttggtgatgg tggtaatgat attagcatgt ttaaacaaac aaactacagt tttgcgatgg
     1081 ggcaagcact tgaaagtgta aaaaatgaag caaagtatat tactttatct aacgatgaag
     1141 atggaatata tgaagcatta atgaaattta atgttattaa aggagtttga gatggaaaat
     1201 aaaatacttg aattatacgc aaatcgaatt aggaaacata ttctaaccga agtatatagt
     1261 gcaaaatcag gtcatccagg aggatcgttg agtgctgctg atatcttgac agaactatac
     1321 tttgaacaga tggatatcaa taaggataat gtaaatacaa tcgatcgtga tcgttttgta
     1381 ttaagcaaag ggcatgcaag tcctgtattg tatgcgacat tagctgaaaa gggatttgtt
     1441 gatattgaag atttaaaagg atttagacat attgattcta aactacaagg acaccctaat
     1501 atgaattatg tatcaggtgt agacatgtct actggatctt taggacaagg aatttcttgt
     1561 gcggttggta tggccatcgc aaataaggta gatgaaaatg atcatcgtat ctattgttta
     1621 ttaggtgatg gcgaatgtga ggaaggtgaa gtatgggaag cagctatggc tgcgagccat
     1681 tataagttag ataacttatg cgcgattctt gattataatc atcttcaaat tgatggaaat
     1741 attgatgatg tgatttcacc tgaaccattt aaatctaagt ttgaagcatt tggctggcga
     1801 gtgattgaag ctaatggaca tgattttaat tcattgcgta cagcatttaa ttttgcaaaa
     1861 caaacagtag ataaaccaac aatcattatt gcccatacta ttaagggtaa aggtgtatct
     1921 tttatggaaa ataactatgc atggcatgga gcagcaccaa accaagaaca atatgaacag
     1981 gcaatgaaag aattaggagg tcttgaataa tggcaacagc aacaagagaa gcatatggaa
     2041 agacattggc aaaactaatc gtagaacatg atgatctatt tgtgttagat gccgatctta
     2101 caaaatcaac aaagacaatc gatgccaaaa aggcaagacc agagcatcat ttcaatatgg
     2161 gaattgccga aggaaatatg atggcagtcg ctgctggtat ggccgcaagt aataaggttg
     2221 tttatgcatc aagttttgcg atgtttgcag caggccgagc ttttgaacag attcgtaata
     2281 gtatttgtta tccaaacttg aatgtaaaag tatgtgcaac ccatgctgga atcagtgtcg
     2341 gagaagatgg cgctagccat cagtgtattg aagacttagc tcttatgcgc tctattccaa
     2401 atatgaaagt attccaacct tgtgatgcta aacagactga agaaattatt aaggctgtat
     2461 actctattga aggaccttgt tatgtaagac taggaagact tggagttgag gatgtatatg
     2521 atgaaaatta caagtatgaa tttggcaagg gaaatgtact taaaaaagga agtaaggtag
     2581 ctatcattgc ttctggatta atggtacagg aaagtttaaa agcatatgat ctattagaaa
     2641 ataagccaac aatcattgat attccaacta ttaaaccaat cgataaggaa ttgatcattg
     2701 aaacagcgaa aacacatgat gtgattgtaa ctgtggaaga acacaatgtg tatggtggtt
     2761 taggtagtgc agttagtgaa gtgcttgcac cagaaggaat tgcatgcaga caatatatgt
     2821 tgggtatgca ggatacgttt ggtagaagtg gaaaaccgaa agaattatta gattattatg
     2881 gtttagatgc aaagcacatt gcagagaaaa tcaaagaaat agttaaatag tataaaagta
     2941 caggctgaat tttgaatgtt cagtctgttt ttgtattaaa tttacatgtg gatttgttat
     3001 acttgaatat ataaggatta gttgaagaaa ggaagagaga tagtatggac tatgcaatga
     3061 aagtatatcc tatgcatttg gaaaatggaa aaacagaatg gtgtgttgaa tatccagatt
     3121 taaaaggatg cgtaggtggt ggagatacaa ttgaagaagc aattgccgat gcagaagcta
     3181 ctaaagctgt atatctaaat tatttaaaag aaaatgaaaa agaacaatgc tctgactcat
     3241 gagcattgtt caagttaata cagaatataa aaatcattct cataataatc aagtggaatc
     3301 tctttttcag tcaaggaatc aatttgaata cgatcaatat tttgaagaga taaaatgact
     3361 ttatcaatgt tttccaagct tccctgaagt tgtgctgtaa cactgccatc atcattgttt
     3421 ttggcatagc ctgtaatacc taatggtttg gcatgacttt gtacttcaaa gcgaaagcct
     3481 atattttgaa catttccttc aaaatgaaac tctttgcgga ttgttcctgg tggcaattct
     3541 ctttttattt tctctttctt tttaaataat ctgaacataa tatatatcct ttactgtata
     3601 tattataagt ctaaatttta acaagaaaaa actttttctg tgaaaaagtc acacgtctaa
     3661 atgacgtgcc cggttcgttt ccagtcgtcc ggtaacattt caatatccac tccatctttc
     3721 tgaatcattc ggtatcccga tccattcagt ctttattgat ggagttttta ttgtatgctt
     3781 ttttataaat acttcagaat ttcatccatg atctatgtac ctatcatcca gtaagatatc
     3841 ataaacttct ggttctgatt atttatctta tttaatccac aaatcccatg cctcttcgat
     3901 tgatcacata ggctgcacaa taatgtacac tcaatccttt gatttttgta tacttttctt
     3961 ttccaatctt actcgtattc tttggatcta ctttgattac atccacttca ttcatcaaac
     4021 attttctttc tactttttct aatatcttct tatacgcaaa actcgataat gttttatttt
     4081 attttttagg acgatagagc attctagttt tcaactgttt gaaattcaag tcttcaacta
     4141 caagactctt ctttttggat ttacattgtt ccatgatttc aatggccagc tgatacagtt
     4201 cttcatttcg tttgttttta gtattttctt tatggattgg atatttcttg atcaatacga
     4261 tatttccatc cttattggtt tcacatagtg ctatatgatc caaattgata tcgattccta
     4321 ttgctccata caatgtatct gtctttggaa gattcatatg tttttcaaat atagctttca
     4381 caataaagta ttcaccataa tccatcaact cataggctac agccttatca ggtgagttgt
     4441 gcttttcatt gactcttgca tacaattcat ccttgtactt atgaaactgc accttgaaca
     4501 cgatgtcttt ctgagtactt cgatatgtca acatatcagt atccacattt aatttgaaaa
     4561 ggttgtttga atacttgccc tgtcttctac cagtaataag taatcttctt gatcttttaa
     4621 aacgataggt ttctaaatca ccatttttaa gattcttttt accaccaaaa catacagaag
     4681 gcatctttct tttaagtttg aactcattcc gatttcttct gaattttaga ttgtgaatga
     4741 tgctcttcaa tcttttgatt tttggatcca atacctgcac ctcatacaag taatcttctt
     4801 ctgtatattt ctttttctta gattgattaa tcaatttctc tttttccttt aaagcttttt
     4861 tcaattgttt ctcgttcttt ttgattttct tgtttatctt tttgatacga gtcttgatca
     4921 aatcacattc ttccttttct ctacatttaa gactcttcac caaagccttt gcctcataaa
     4981 tggcagacaa aggaaaataa tcatttgtac catagaatga cttgatcatc ctttgatcag
     5041 tttctgaata ggtagcatcc ttataggcac gatcaaaaca gattttgaac gccttatgaa
     5101 gtatacagtt atacagttca agatcctttt taatgatcaa ttctgcatct ggttccaaat
     5161 ctttataata gattctttgc gacattgaaa acaccttcat aagacaaatc ctcctgttct
     5221 atctgacttt attatactat atttaatgca agcttaatag atagatttga gactttctaa
     5281 tttattttat ttatatcatt atatcaccaa tgtgaatatg tgatatttat tgatattctc
     5341 acatgttcac attaaattca cattgacact gcatttttat tcaatttaga ataatttcat
     5401 tctatacttc aattatggac actacttatc attcaaacat tcaagttaaa actttatctc
     5461 ttttagattg tgatgtgaga aacttcaatt ttattcatcc catcaagaac atcttttttg
     5521 ataatattga ctagatgctt ccggtaaaac tcgtccttgt attttaaaca atgtagaacg
     5581 ttctcttctt tgccacacat gttatcttgg ttttgaccaa tttgaatgtc ccgattgtga
     5641 caattggaac attatcccac attcttgtca ttctagattt tgtaatgcct gtggtgttaa
     5701 atacgccaaa cagcttgcag ccaaggttac ctctttttgt cttgactgcc ctcataagca
     5761 cattgtcttt actattcctg aagaacttag aaactggttc agacaggatc gttctagact
     5821 taatcttttg tttgttgctt ccagaaacat aatttctatt cttaccaata aatctcttgc
     5881 cgataaactc aaaaagaaaa agctttctga tactcactat atattcaagg atattcctgt
     5941 tcgtaatgaa tttggcatga ttgccactat acatactttt acaagacctt aaatggaatc
     6001 cacacatcca ttgccttatt cctgaattaa tctattcctt taaaaaggat aaaatcaaaa
     6061 catttcatca tttcaatttc aagaagctta gaaaaacatt tcagtttgaa ctgattcgcc
     6121 ttattcaaga agctggtgga ttaaagaaac ctgaagaaaa ctatatgatg cactgccctg
     6181 aatgtgatca taagatgatt gtaaaaaaat aattcaacaa ctaaaaatga atacaaaatg
     6241 ttggctattt tcaatagtct tttttgtcgt tcctaataat tcatatcttg tgatatttta
     6301 gcgctatttc acataccgta tatccctgtt ccgaaaccaa aaatcttaat atataaaaaa
     6361 agaccgatat tcatagggaa aatagaatat cgatctttct tttacatata gtaacagata
     6421 actacgatta ggatatttta atgtaagtat attgttggaa taggagaaat aaatacatga
     6481 tagtaatctg ttgctgccaa ataattgaat tatctttgga cgcggcctga accgtctcca
     6541 ccagctaatt gcaacacttc gtccatactc actatgttga aatcaccttc gatagagtgt
     6601 tttaaacaac ttgcagccac cgaaaattcg attattgact ttgcatcata tccttctaga
     6661 cttgttgcaa ccaatccagc accaaagcta tctccaccac cgactctgtc tacaatctgc
     6721 atcttgtatt tatttgagaa atagtaatct tttctatcat acaacatagc ggaccagttg
     6781 ttgatattgg cattgataga ctctcttaat gtgatagcta cttccttata tccttcgcga
     6841 ttccaatagg atatccatat ttaataatag gtcgctcttt ttaatatctt tcaacgtaaa
     6901 tttatgtctt tgggcaatat cttcaataag tgtaatttct tgtccatcga cattacatat
     6961 agtccctttt tctagaggac atgatgcaac agctaccaag tcgttaaaat gaatcttata
     7021 aatttttgca ccatttctac ctgtctatat tttaaatgtt agatgtaagt acttacacag
     7081 aacaaccctg ttttttgaat aaatcaatag aaacatttaa aaagcttaca aataagtatg
     7141 tttcatatct gttttttgat aaaatgtaga aaaagagata atacccctta aatacaagtg
     7201 tgaaaacgat tccgaaaaaa cttgacttat gaaatgtgag ggactacaac aagaaatatg
     7261 atgtaagtac ttgcaattct cagtgcgttg gaatagggtt atggagagta tccttatgat
     7321 aaggaaaact taactgaaat tgttaggggt atctcttaca acaatgccgt tcgttatttt
     7381 ggatttgatt tatagtagtt aatgaaaaag ggaactttga ttcgaggttc tctttttttc
     7441 gtaataatga acatatggag ttaattatgt tgataagtga agtaatatca taagggtggt
     7501 actatatttg ataaagttaa ttacatttgt gttgcaaaac ttgaaatgac aagaaccttg
     7561 aacaactgca atttcaagct ttaataatat tggggagcaa aatatggctg gaacagcagg
     7621 atgtactccc caatatgaat gttgcttaag actctattgt tttccagcag cgctctttca
     7681 taaatgctgt aattactgcg atgtcttcac cttcgtagta ttttaccagc aaccgcttga
     7741 attcaggtac ttctttttca ggaattacta gaaagccacc gccatgtgaa ataagataat
     7801 ggtttgcaaa aataacagaa gcacgtttat taccattcaa aaatatttga gccttcatgc
     7861 agtaaaggca cagtttgatg gagatattaa tgggttcatc atcttcccca gcaatctctt
     7921 gaattttatc ttttacatcc agttcattcg gcaaaaaagc cttcatttac aattcttgca
     7981 atatgactga gcatataata atcggaacgg ctggcaacta catcgcggtc caaaataaac
     8041 tcccatgcat gtttcagatt taaaatcttc tgcacatcag tagcggttat gccggaaacc
     8101 tttccgtttt ctatgatttc ctctgtctgt ggaaaggacg ttgcgacacc ttccaaaaca
     8161 gcctgatcat agatgttcat cttcatattg gcatgtgcga atgcaatgtt gtttacgaca
     8221 ttaatagaaa gttcgtcttc agaatatccg gcttcagcaa gttgtttttc aatgcttcgg
     8281 agtgattttc tgatttcacg aatttctctt gcatttcgaa gaagcaaatt atacagttct
     8341 tcagtatacg cacccacgta agttgatgtc tgcttgccag caacacgttt tcttacatag
     8401 aggtattttt tcgcaccacg ttctttgatt tcaggtgttc catcgtatgg catcaaatta
     8461 agcctagcgt gaagatcggc gcgacttctg agcaattctt gtatttcact atattgtacg
     8521 ggcatgattt ctcctcctta tagggaacaa ttattacgat tataatacaa caatgctccc
     8581 caaaactata aattcaagac atagtatgtc caaatcgcac tatattcaaa taaaaaggat
     8641 tcctctgtca ttgtagacag aagcactgga tgtccattgt gggacagctt ttgttccagc
     8701 gttagcttca taagttcttt tgttagggtg acacattctt aactccatga ccgaatggaa
     8761 caacagtaaa gatcacgcct ttacggttct gtacaaactg tacagttccc cagcattcaa
     8821 aggaaatttc tctgatattg aagagttcac caagatgctc aaggaacttt tcgatgtagc
     8881 cgtagaggat aactttcctt ttgaggtttc cagatagctt tgtcggttcc agatatcgta
     8941 taagacctga aaatacaact ttgtattaat caaacaatgg aagttgaagc aagaagctaa
     9001 gttgtttgta gccgagtagt tcactacttt aagtaagtaa aaatactcaa aggtgaaaaa
     9061 tcagttgaaa atattctttt gaaggtgtca taatttaaaa aaatgacact ttttct
//
LOCUS       DBMDWL010000079         9037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2763_length_9037_cov_2.169203, whole genome shotgun sequence.
ACCESSION   DBMDWL010000079 DBMDWL010000000
VERSION     DBMDWL010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2763_length_9037_cov_2.169203"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="ACZPDN_06580"
     CDS             complement(<1..812)
                     /locus_tag="ACZPDN_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR1298321.1"
                     /translation="MLIQDDALRSVIARTLRVKEEELTEDLMKELIHLEVQDHEIATL
                     EGLQYAENLETLNASNNKLQTLDPIRDLHNLVCLDVSRNQLRDLQALHGFRQLKKLNV
                     SRNNLYTMDISSVAAMIDLEVLNLSKAKVDSLIYLEHCKKLEEVHINTENGPFSYAIL
                     GVLKKLKKLDMGGMRLFNIEDLTYLSNVEELDLNTNLMSDLSPLLSMKNLKKLNVSNC
                     PYLKDYSVLKEFSNLKSLNISYNEPDHFMFLKELNHLESLSMEQTGFKDMSL"
     gene            1031..1243
                     /locus_tag="ACZPDN_06585"
     CDS             1031..1243
                     /locus_tag="ACZPDN_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR1298322.1"
                     /translation="MSTGKVKWFNAEKGYGFITSEDGKDVFVHYSSINSEGFKTLEEG
                     QTVTYDVVESDRGQQANNVTVVDTAA"
     gene            1302..1772
                     /locus_tag="ACZPDN_06590"
     CDS             1302..1772
                     /locus_tag="ACZPDN_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1298323.1"
                     /translation="MLERYEKVKEYVYGQYAKIYNEDLKIAALTHTASVDLSITFIAM
                     ARGTNLERAKVAALFHDYAQFVDNCPHSQHAKLSSLHAYKYLKECGLFKTTEIDDITY
                     AIRQHSKKESFDSPLCEALKDADVLARFLENPEKPLVGMKRQRLLNACADIQSH"
     gene            complement(1769..2812)
                     /locus_tag="ACZPDN_06595"
     CDS             complement(1769..2812)
                     /locus_tag="ACZPDN_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298324.1"
                     /translation="MSAYTNGLVFWKHFEKKQDAIFNYLKSEQYEELNDIIQELDEEV
                     MEMSGAHFFVENFYDSFEMTFDTGPNKTTQYLCQMLCDIAPKSVKQNWIMNACLPAMS
                     QKAIQAMVQIKNEEYTLADFHVFYQIENDMLDCKVYCPGFNLIGNPENKKEMSMYLLE
                     LAIGQLAYEAYICRVDFIDTPDSNMKFCQMMDFYEVIMALVEKNHWKEYDKPIDIYSV
                     YQPIQDFAHDALRKDMKLIFTTHPLLVEQTIEDKEEVLADLSSKDGEFGYVYYSNPFH
                     NKEDALYRQKLSKELDEAISKVHAGKVVGGAIGKSFSYIDWIVYDKALFMKVFNQLKK
                     QLDASVELYYQKF"
     gene            complement(2829..3449)
                     /locus_tag="ACZPDN_06600"
     CDS             complement(2829..3449)
                     /locus_tag="ACZPDN_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1298325.1"
                     /translation="MKILFYGDSNTWGYDAKDASRQKNRFTQFIKQEFSQHEIIEEGL
                     CGRTICLDDPYDEDRNGTKMISMVLKTHAPIDVVFIMLGTNDAKRQFSTNSISLEKGI
                     RTLLYKALNPEIYRDGSKVPQFFVVCPPKMNRDGLNNERTKENFGDAGFEILNNTKPY
                     LEKGCRDFDVEVIDTHAIAGSIDGIHMDESGHKQVADVLISVIQEL"
     gene            complement(3446..3943)
                     /locus_tag="ACZPDN_06605"
     CDS             complement(3446..3943)
                     /locus_tag="ACZPDN_06605"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1298326.1"
                     /translation="MDKRKECIQRRSLVSNAERLLKEKAITNCVLPFLKGKIGTYIPI
                     RNEVDVYSNFKEEFDLYIPKMLDDTTIAFYSDKEGKINGPFSTIEPIGICEEKDLNVI
                     LVPVVGFFDTYRLGYGKGYYDRYLKDSSALKIGIAFDVQEIKFDVKPTDVKLDMIITE
                     SRILR"
     gene            complement(3936..5348)
                     /gene="sufB"
                     /locus_tag="ACZPDN_06610"
     CDS             complement(3936..5348)
                     /gene="sufB"
                     /locus_tag="ACZPDN_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR1298327.1"
                     /translation="MKKMSNEKDIALSSEYEYGFHDEDVSVYKTQKGLNEETVRTISK
                     IKKEPEWMLQLRLDAYHSFVEQQNPNWGPALSEIDFDDYTYYIKPSDKQEKSWDEVPD
                     TIRNTFDKLGIPEAEQKFLAGVSTQYESEVVYHNMLEEVEEKGVIFLDTDSALRTCPD
                     LLKKYFGKLVPYTDNKYAALNTAVWSGGSFIYVPKGVKLEKPLQSYFRINSMSMGQFE
                     RTLIIVDEGADVHYVEGCTAPTYSKDSLHAAVVEIYVHKNAKCRYSTVQNWSSNILNL
                     VTKRAKVFENGHMEWIDGNVGSHVNMKYPACILAEPYAKGTTISIAVAGENQIQDAGS
                     KMIHLAPHTSSTIISKSIARNGGEVNYRGWVTHSKNAAYAKSKVECDTLILDKKSRSD
                     TVPMNVSYNDTSQIEHEATVSNISEEQLFYLMSRGLTRSQATEMIVMGFLEPFTRELP
                     MEYAVELNQMLKLDMSDSIG"
     gene            complement(5329..5778)
                     /gene="sufU"
                     /locus_tag="ACZPDN_06615"
     CDS             complement(5329..5778)
                     /gene="sufU"
                     /locus_tag="ACZPDN_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR1298328.1"
                     /translation="MSNIDPMLMRQIIMDHYDYPRNKELTDNEGYMSRHMASDSCIDD
                     ITVQTKISDGKIDDIRFDGVACTISTASTSIMSELLKGKTTEEAKEIMDNYFKMIDQK
                     EYDEDVLDEAIAFQNVGKQANRINCATIGWKAMREMIKESEDEKDEQ"
     gene            complement(5794..7035)
                     /locus_tag="ACZPDN_06620"
     CDS             complement(5794..7035)
                     /locus_tag="ACZPDN_06620"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1298329.1"
                     /translation="MDISKIRKDFPILTRTMSNKLLVYFDNGATTLKPQPVIDAVVNY
                     YTYLGANAHRGDYEMSAQVDAAFEGARVRTQKFLNAKYKEEIVFTSGSSEGLNLVAMM
                     VTQQLLNEGDVILSDESEHASSILPWMQAGKKKGTKIDYIPLDEEGKITVENFKRVLT
                     DKVKVVAIAMVSNVLGFVAPVKEIAKICHERGILLVIDGAQSTPHLNIDVQDLDCDFY
                     AFSAHKMCGPTGVGALYGKKKWLDQLEPIFYGGESNARFDKSCSLTLKETPLKFESGT
                     QPIEGVIGMAAAMDYLDSIGKENIHAHEVELKEYFLEKAKALGNIVVYNENARSGIVT
                     FNVKDKDQMIFAQDVASYLNTFGIAVRSGQHCAKLLPDVLKTPGTCRASFYLYNTKEE
                     VDQLIDALKNATVENCVSIFF"
     gene            complement(7022..7795)
                     /locus_tag="ACZPDN_06625"
     CDS             complement(7022..7795)
                     /locus_tag="ACZPDN_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HIR1298330.1"
                     /translation="MLNRFDVHNSETLNIQIDRHDQKSYLIELSKDCTKLDLRVECLE
                     NSFSKFFILNHAENACELNINMVLKQDANCQMGVLDLEKSPLKWNHYVDLKEPGAEYE
                     ILSGQLCQEHIEKVCDMEVRHNAPHTNGQMKNFVVLFDHGQYQMVANGNIKKGCFDAQ
                     SHQATRVLTLGQGHKTKVIPLLLIDENDVKASHALTIGQPDADQLYYLQSRGLSTKQA
                     VGLLSVGYFLPVIDLVEDEELKNSLRQEMESKVGLYGHQ"
     gene            complement(7788..8600)
                     /gene="sufC"
                     /locus_tag="ACZPDN_06630"
     CDS             complement(7788..8600)
                     /gene="sufC"
                     /locus_tag="ACZPDN_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR1298331.1"
                     /translation="MADLVIKDLHVSVENKEILKGLSLTVKSGERHALMGPNGNGKST
                     LLAAIMGNPKYTVTSGSITLDGKDVLQMSVDERSKAGLFLGMQYPSVVSGVTNSDFLR
                     SAMNARLEKPISLFKFIKEMDKAIEQLEMKKDLAHRFLNDGFSGGEKKRNEIVQMMML
                     KPSIAMLDEIDSGLDVDALRIVSDAINQCQKQTDLGLLIVSHYARFYEYLQPTHAHVL
                     MDGQIVVSGDASLVEKVDQEGYEWIEKEYHVTANKEDKQPISLGSCGAKKYA"
     gene            8733..>9037
                     /locus_tag="ACZPDN_06635"
     CDS             8733..>9037
                     /locus_tag="ACZPDN_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1298332.1"
                     /translation="MTDILRNNWFVVLIAIIIIGFIGYFIYDTNKDNVSAKSSNNEQV
                     VASIDKDDITANDLYDESAPYDGSTIYNMYKNAVIDQSIETTKDIKKQANTLESNIKS
                     "
BASE COUNT         2887 a         1808 c         1240 g         3102 t
ORIGIN      
        1 aataatgaca tatctttgaa tccagtttgt tccatagata acgattctaa atgatttaat
       61 tcctttaaga acataaagtg atcaggttca ttataagaaa tattcaatga ttttaggtta
      121 gaaaattcct ttaatacaga ataatccttt aaatatggac agtttgaaac atttaatttc
      181 tttaagttct tcatcgataa taatggactt agatcagaca ttaagtttgt atttaaatcc
      241 aattcttcaa cattcgaaag atatgttaaa tcttcaatat tgaataatcg cataccaccc
      301 atatctaatt tttttaattt tttaagtaca cctaagattg catagctaaa tggaccattt
      361 tctgtgttga tatgaacttc ttctagcttt ttacaatgtt ccaaataaat caaactatct
      421 actttagcct ttgaaagatt caatacttcc aaatcaatca tggctgcaac acttgaaata
      481 tccattgtat ataagttatt tcttgataca tttaattttt taagttgtcg aaagccatgt
      541 aaagcctgca aatctcttaa ttgatttcgt gaaacatcta gacaaactaa attatgtaaa
      601 tcacgaatgg gatccaatgt ctgtaacttg ttgttacttg catttaaagt ctctaaattc
      661 tctgcatact gcaaaccttc tagagttgct atttcatgat cctgtacttc taagtgaatc
      721 aattctttca tcaaatcttc ggttagttct tcttctttaa cacgtaatgt acgcgcaata
      781 acactacgca aagcatcatc ttgaataagc ataaaacaca tgccctcctt tttatataat
      841 cattataaac aaaatatgac acaataacta tacaaaaatg ttatttttga atgacattat
      901 tgttagaaaa tgctaaaatg aaagcagtta aggattagac tcatattctg attgtgataa
      961 atgctattgt atgcaagtac tttcgatttt aacggtcaaa ggatattggc ttagtccagg
     1021 aggtataggc atgagcacag gtaaagtaaa atggtttaac gctgaaaagg gttatggatt
     1081 cattaccagc gaagatggaa aagatgtatt cgtacactat tcatcaatta attctgaagg
     1141 atttaaaact ttagaagaag gacaaactgt tacttatgac gttgttgaaa gcgatcgtgg
     1201 tcaacaagct aacaatgtca cagttgtaga tacagctgcg tagaaatttg ttcgaatcaa
     1261 gcgcacatcg tgcgcttttt tcatgaaaga gagggattat tatgttagag cgatatgaaa
     1321 aagttaaaga atatgtatat ggtcaatatg ctaaaattta taatgaagat ctaaaaattg
     1381 cagcactcac acataccgca agtgtggacc tttcgattac attcatagct atggcaagag
     1441 gcacaaatct agaacgtgct aaagtggctg ctcttttcca tgattatgct caatttgttg
     1501 ataattgtcc ccattcacaa cacgcaaagt tatctagtct tcatgcatat aaatatttaa
     1561 aagaatgtgg tttatttaaa acaactgaaa ttgatgatat cacatatgcg attcgtcaac
     1621 attcaaaaaa agaatctttt gactccccat tatgtgaagc cttaaaagat gcagacgtat
     1681 tagctcgatt tttagaaaat ccagaaaaac ctcttgtagg aatgaaaaga cagcgcctgc
     1741 tcaatgcctg cgctgatatt caaagtcatt aaaatttttg ataatacagt tcgacacttg
     1801 catcgagctg tttttttaat tggttgaaaa ccttcataaa caaggcttta tcatacacga
     1861 tccaatcaat ataggaaaaa cttttaccaa tagctcctcc aaccactttt cctgcatgga
     1921 ctttagaaat tgcctcatcc aattcttttg ataacttttg acgatataaa gcatcctcct
     1981 tattatggaa aggatttgaa taatacacat atccaaattc accatcctta ctgcttagat
     2041 ctgccaaaac ttcttcctta tcctcaatcg tttgttccac caataaagga tgtgttgtaa
     2101 aaatcaattt catatctttt cttagagcgt catgcgcaaa atcttgaatt ggctgataaa
     2161 ccgaataaat atcgattggc ttatcatatt ctttccaatg atttttttca acaagtgcca
     2221 tgattacttc ataaaaatcc atcatttgac agaatttcat atttgaatct ggcgtatcta
     2281 taaagtcaac acgacaaata tatgcttcat aggcaagctg gccaatcgca agctctaata
     2341 aatacatact catttctttt ttattttcag gatttccaat taaattaaac ccaggacaat
     2401 atactttaca atccaacata tcattttcga tttgatagaa aacatggaaa tcagctaaag
     2461 tatactcttc atttttgatt tgaaccatag cctgaatcgc cttttgcgac attgcaggta
     2521 aacaagcatt catgatccaa ttttgtttta cagattttgg tgcaatatca caaagcatct
     2581 gacatagata ttgtgtcgtc ttattaggac ctgtatcaaa ggtcatctca aaagaatcat
     2641 aaaaattttc gacaaagaaa tgggcaccac tcatttccat cacttcttca tccaattcct
     2701 ggataatatc gtttaattct tcatattgtt ctgattttaa ataattaaaa atcgcatctt
     2761 gcttcttttc aaaatgcttc cagaacacta aaccatttgt ataagcactc attaagtatc
     2821 ttcctttcct ataattcttg aatcacagat attaacacat ccgcaacttg tttatgacca
     2881 ctttcatcca tgtgaatacc atctatactt ccagcaatcg catgcgtatc aatcacttct
     2941 acatcaaagt cacgacatcc tttttctaaa taaggctttg tgttatttaa tatttcaaaa
     3001 ccagcatccc caaaattttc tttcgttctt tcattattca atccatctct attcattttt
     3061 ggtggacaaa ccacaaaaaa ttgaggcact ttcgatccat cccgataaat ttccggattc
     3121 aaagccttat ataataaggt acggattccc ttctctaatg aaatggaatt tgttgaaaat
     3181 tgacgcttcg catcatttgt acccaacata atgaacacaa catcaatggg agcatgtgtc
     3241 tttaaaacca tcgaaatcat tttagttcca ttgcgatctt catcatacgg atcatctaaa
     3301 catatggttc ttccacatag accttcctca atgatttcat gttgactaaa ctcttgttta
     3361 atgaactgtg taaaacgatt cttttgtctt gaagcatcct tggcatcata gccccaagta
     3421 ttgctatccc cataaaataa tattttcatc gtaatatcct cgattctgta ataatcatat
     3481 ctaacttaac atccgttggt tttacatcaa actttatttc ttgcacatca aaagcaatcc
     3541 cgatcttcaa tgctgaacta tctttcaaat acctatcata atagcctttt ccatacccaa
     3601 gacgatatgt atcaaaaaag cctacaacag gaactagaat cacatttaaa tctttttctt
     3661 cacaaattcc aataggttca atcgtagaaa aagggccatt tatttttcct tctttgtctg
     3721 agtaaaaggc aatcgtagta tcatctaaca tctttggaat ataaagatca aattcctctt
     3781 taaaatttga atatacatcc acttcattgc gaatcgggat ataggttcca atttttcctt
     3841 ttaaaaaagg caaaacacaa tttgtgattg ccttttcttt taaaagtctt tctgcgtttg
     3901 atactaaact acgcctttga atacattctt tacgcttatc caatgctgtc actcatatcc
     3961 aacttcaaca tttgattcaa ctcaaccgca tattccattg gaagttcacg tgtaaatggt
     4021 tccaagaatc ccattacgat catttctgta gcttgtgaac gagtcaaacc acgagacatt
     4081 aaatagaaca attgttcttc actaatattt gaaacggtag cctcatgctc aatttgcgac
     4141 gtatcattat aagatacatt cattggaact gtgtcactac gagatttctt atctaagatc
     4201 aatgtatcac attctacttt gctcttagca tacgcagcat tttttgaatg tgttacccaa
     4261 ccacgataat tgacttctcc accatttcgt gcaatcgatt ttgaaatgat tgtacttgaa
     4321 gtgtgtgggg ccaaatgaat cattttacta cccgcgtcct gaatttgatt ttcacccgct
     4381 accgcaattg aaatggttgt tccctttgcg tatggttcag ccaaaataca agctggatat
     4441 ttcatattca catgtgaacc aacgtttcca tcaatccatt ccatgtgtcc attttcaaat
     4501 acttttgcac gttttgtaac taagtttaag atgttgctag accagttttg tacagttgaa
     4561 taacgacact tcgcattctt gtgtacatag atttctacaa ctgccgcatg taaagaatct
     4621 tttgaatacg ttggtgccgt acatccttct acataatgca catctgcacc ttcatcgaca
     4681 ataatcaatg ttctttcaaa ttgtcccatc gacattgagt taatacggaa ataagattgt
     4741 agaggttttt ctaacttcac acccttaggt acataaatga aagatccacc agaccataca
     4801 gctgtattta gagctgcata tttattgtct gtataaggca ctaatttacc aaagtatttc
     4861 tttaataaat ctggacatgt tcttaaagcc gaatctgtat ctaagaagat aacacccttt
     4921 tcttccacct cttctaacat gttgtggtaa acaacttcac tctcatattg tgtagaaact
     4981 cctgctaaaa acttttgttc tgcttctgga attcctaatt tatcaaatgt attacgaatg
     5041 gtatctggca cttcatccca agatttttct tgtttatcac tcggtttaat atagtaagtg
     5101 taatcatcaa aatcaatttc acttaaagct ggtccccaat ttggattttg ttgctccaca
     5161 aaagaatgat atgcatccaa tcgcaattgc aacatccatt ccggttcctt tttgatttta
     5221 gaaatcgtac gtaccgtttc ttcattcaaa cctttttgtg ttttatatac acttacatct
     5281 tcatcatgaa aaccatattc atattcacta gaaagtgcga tatctttttc attgctcatc
     5341 tttttcatcc tcactttcct taatcatttc acgcatagcc ttccaaccaa ttgtggcaca
     5401 attgatacgg ttcgcttgtt taccaacatt ttgaaacgca attgcttcat ccaaaacatc
     5461 ttcatcatat tctttttgat caatcatttt gaaatagtta tccataattt cttttgcttc
     5521 ttcagttgtc tttcctttta ataattcaga cataattgaa gttgaagctg tagaaatcgt
     5581 acaggcaaca ccatcaaaac gaatatcatc aattttccca tcggaaattt ttgtctgcac
     5641 cgtaatatca tcaatacaag aatcactcgc catatgacgt gacatataac cttcattatc
     5701 tgtcaattct ttattacgag gatagtcata atgatccata ataatttggc gcatcaacat
     5761 aggatcaata tttgacataa ttaagcttcc ttcctaaaag aaaatgctga cacagttttc
     5821 tactgttgca ttctttaaag catcaatcaa ttgatcgact tcttcttttg tgttgtacaa
     5881 atagaaactt gcacgacaag tacccggtgt ttttaataca tccggaagta atttagcaca
     5941 atgctgtccc gaacgaacgg caatgccaaa cgtatttaaa tagcttgcga catcttgcgc
     6001 aaaaatcatt tgatctttgt ccttcacatt aaatgtcaca ataccacttc tcgcattctc
     6061 gttatataca acaatatttc ctaaagcttt agctttttct aaaaaatatt cttttaattc
     6121 aacttcatgc gcatgaatgt tctctttacc aattgaatcc aaataatcca tggcagccgc
     6181 cataccaata acaccctcaa ttggctgcgt tcccgattca aacttcaatg gagtttcttt
     6241 taaagtcaat gaacaagact tatcgaatcg agcattactt tctccaccat agaaaatcgg
     6301 ttctaattgg tccaaccatt ttttcttacc atataaagcc cctacacctg ttggaccaca
     6361 cattttgtgt gcactaaagg cataaaaatc acaatctaga tcttgtacat caatatttaa
     6421 atgtggtgta ctctgtgctc catcaatcac aagcagaatg cctctttcat gacagatctt
     6481 ggcaatttct ttaactggag ctacaaaacc taaaacgtta gataccatcg caatcgctac
     6541 gacttttacc ttgtctgtta aaacacgttt aaagttttca acggtaatct taccctcttc
     6601 atctaaaggt atataatcaa tttttgttcc ctttttcttt cccgcttgca tccaaggtaa
     6661 gatgcttgaa gcatgttcac tttcatcact taaaatgaca tcaccttcat ttaaaagttg
     6721 ctgagtaacc atcatcgcaa ctaagttcaa cccttcgctt gaaccagaag taaaaacgat
     6781 ttcctcttta tatttggcgt ttaaaaattt ttgtgtacga acacgagctc cttcaaaagc
     6841 agcatcaact tgtgcactca tttcataatc accacgatgg gcattcgcac caaggtatgt
     6901 ataataattc acaacagcat caatgacagg ttggggcttt aatgtagttg ccccattatc
     6961 gaaatatact aagagtttat tggacattgt acgggttaaa attggaaaat ctttacgaat
     7021 tttactgatg tccatacaat ccaaccttac tttccatttc ttgacgaaga ctgttcttta
     7081 attcttcatc ttctactaaa tcaatgacag gtaagaagta acctacactt aataagccaa
     7141 cagcttgttt tgtacttaat ccacgagact gtaaataata taactgatct gcatctggct
     7201 gaccaattgt aagggcatga cttgccttta catcattttc atcaattaat aataatggaa
     7261 taactttcgt cttgtgtcct tgacctaaag ttagtacacg cgttgcctga tgagattgtg
     7321 catcaaagca tccttttttg atattgccat tggcaaccat ttggtattga ccatgatcaa
     7381 atagaaccac aaagtttttc atttgaccat ttgtatgcgg tgcattatgt cttacttcca
     7441 tatcacatac tttttctata tgttcctgac acaattgacc acttaaaatt tcatattctg
     7501 cacccggctc ttttaaatct acataatgat tccattttaa cgggcttttt tctaagtcca
     7561 agacacccat ctgacaattg gcatcttgtt taagtaccat attaatattt aattcacatg
     7621 cattttcagc atgatttaga ataaagaatt ttgaaaaaga attttctaaa cattccactc
     7681 ttaaatctaa ttttgtgcaa tctttgctta attcaatcaa ataagatttt tgatcatgac
     7741 gatcaatttg aatatttaaa gtttcactat tatgaacatc aaatcgatta agcatatttt
     7801 tttgctccac aagaacctaa agaaataggt tgtttatctt ctttatttgc ggttacgtgg
     7861 tactcttttt caatccattc atatccttct tgatcaactt tttcaacaag gcttgcatct
     7921 ccagatacaa caatttgtcc atccattaat acgtgagcat gtgttggttg taaatattca
     7981 tagaaacgtg catagtgaga tacgatcaat aaaccaagat ctgtttgttt ttgacattga
     8041 ttgatggcat ctgaaacaat acgcaatgca tccacatcaa gtcctgaatc aatttcatcc
     8101 aacatggcaa ttgatggttt taacatcatc atttgaacga tttcattacg cttcttctct
     8161 ccaccactaa atccatcatt taagaatcgg tgagctaagt ctttcttcat ttctaattgc
     8221 tcaatcgcct tatccatttc cttaatgaat ttaaataatg agattggttt ttctaaacgc
     8281 gcattcattg cacttcttaa aaagtcagag tttgtaacgc cagaaacaac acttggatat
     8341 tgcattccta agaaaagtcc ggctttactt ctttcatcta cagacatttg tagtacatct
     8401 ttaccatcta atgtgattga accagatgta actgtatatt ttgggtttcc cataatggct
     8461 gcaagcaatg tagatttacc atttccatta ggtcccatta gggcatgacg ctcacctgat
     8521 tttacagtta aactcaaccc ttttaaaatt tctttattct ccacggatac atgaagatcc
     8581 ttaatgacca aatctgccat acattctacc tcttttcact gcaatataat accaaataat
     8641 tattaaaatc tcaattaata catctcggcg gttcattgca ttttaaaaag atatctcgta
     8701 taattgaaaa gacattctca aggaggttaa ttatgactga tattttacga aataactggt
     8761 ttgtcgtcct aattgccata attattattg gttttattgg ctactttatt tatgacacaa
     8821 ataaagacaa tgtatccgca aaatcatcaa ataatgagca agttgttgct tcaatcgata
     8881 aagacgatat taccgcaaat gatttatatg acgaatccgc accatatgat ggttcaacaa
     8941 tctacaatat gtataaaaat gccgtcatcg atcaatcaat cgaaactaca aaagatatta
     9001 aaaaacaggc aaatacattg gaatctaata ttaaatc
//
LOCUS       DBMDWL010000080         9034 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2764_length_9034_cov_3.127729, whole genome shotgun sequence.
ACCESSION   DBMDWL010000080 DBMDWL010000000
VERSION     DBMDWL010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2764_length_9034_cov_3.127729"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            154..408
                     /locus_tag="ACZPDN_06640"
     CDS             154..408
                     /locus_tag="ACZPDN_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298333.1"
                     /translation="MDVITILKFEEMVVLYIKILKKTITDTELELFKYNLEISCCVPN
                     AIFFNLNSEAKKILGKKEWIKLYSPDIEREFHSGPIETKS"
     gene            491..1711
                     /locus_tag="ACZPDN_06645"
     CDS             491..1711
                     /locus_tag="ACZPDN_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1298334.1"
                     /translation="MFKKGKRLAALCTIIFVVLSMCMPCLADETQKTIRVGSFEDTFN
                     YVDEHGVRRGYGYELMQALAGYTGWKFEYVKCDWSNCFEKLENGEIDIMGDIAYTDDR
                     AKEMLFSEETMGEEKYVLYANLSDMDIVSSDFKSLDGKRIGVLKGTNSEYMLTNWENK
                     YGIHTEHVNVSGNEDVKKKLANNEIDCFVSLEESIWSERGISSVTTIGESDIYFVINK
                     ERSDIKQELDYAMRQLDSDSPFFKADLYKKYFTLDYTQFLTGFENSYIKEHGGIRIGF
                     LDNDSVVFSMDKKSGEIRGTLSEYISYAKDCLGNHTLKFDIEAFDDYDKMIEALQNNE
                     IDVIYYASRNSNVAEKKGYALSNTAWTYNLIAVTNKEDFNENDAYVVAVPKNKVALKQ
                     HIAYNLSALETRGL"
     gene            1734..3269
                     /locus_tag="ACZPDN_06650"
     CDS             1734..3269
                     /locus_tag="ACZPDN_06650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1298335.1"
                     /translation="MVDDGKVDCFIMGSSQVLSFDGKKNFKIIPLTKTMEACFAVKDG
                     QATLLSILNKTIKAMPSDMLTSTIAMYDSTAYKTTFYSFLKDHLVVVLVLGFSIIIVI
                     LLLLQKARRAEASAKVALKSAEDASLAKTQFLHNVSHDIRTPMNAILGYSELMKNELK
                     DNDMPRVFEHLNKLQKAGNILLSIINNVLDMARIESGHMELDENYANIEDIRQRLIEM
                     FEDEAKKKNIHVRYTFNVEHKHVMADVTKAEEIFVNILSNALKYTPSNGSVEVNVDEL
                     SCDEPGYMIVRTSIMDTGIGMSQEYLEKIFESFSRERMTSSKISGTGLGMAIVKKYVD
                     LLGGTIDVKSVLGKGSTFIVTLKYKLADEKYYLNKQEENCEVDNAIFNGKHILLAEDN
                     DLNAEIAQTILERAGLFVDRVKNGIECVNRVIENPVGTFDVILMDIQMPKMDGYKATC
                     EIRNLQDKAKACIPIVAITANAFEEDKKKAIDVGMDGHIAKPINIEDLFVVLTDILNK
                     QKS"
     gene            3514..4491
                     /locus_tag="ACZPDN_06655"
     CDS             3514..4491
                     /locus_tag="ACZPDN_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1298336.1"
                     /translation="MPTRNEVLIRSANLLNDFAFKYVFGEDCKEANDALKSLLTVFLE
                     RSVNKVVVKNSEIVKDYSKMKSPRLDLLVEFDDQTMVDLEMQLRQTKDNLPIRFSYYL
                     ARLHGSQELEGKYYGELKETIVLVFFNVNLIDNHRMCNTFTLKNEDGLSFVKETEDRM
                     KIRTVEMAKLDINKPLEKMIYYFLNCHKGMDDSKIKVMIESDGVIQMLEKRVETISDD
                     GWKKIIEDFQKLHENEERMELQLEIEEANKQVEEANKRAEVANKQVEEANKRAEVANK
                     RADESDKKMDQMNQVMKQMIQAMLTNGMSLELISKSLSKSEDEIMELLK"
     gene            4801..5307
                     /locus_tag="ACZPDN_06660"
     CDS             4801..5307
                     /locus_tag="ACZPDN_06660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HIR1298337.1"
                     /translation="MSLIKTRRSVQAYKSEPVPSKALDAVLEAGTCAPTGGGYQSPTI
                     IAITDSKYRRKITKLNAEVMVGNTDPYYGAPVVILVLADGSASTYVEDGSCVLENRML
                     AAHALGLGTVWIHRELEIFDSESSKNLLREWKLLETLRGIGAIALGYLAQEAGKPSAR
                     KQNYIVRI"
     gene            5367..6707
                     /locus_tag="ACZPDN_06665"
     CDS             5367..6707
                     /locus_tag="ACZPDN_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298338.1"
                     /translation="MQTNNKMAVAPVGRLIWQMSLPPLISMFLQYSYNLIDSAFIARL
                     SENALAAVSLSFPITTLMNAASIWIGVGVNVLIAGYLGQKKQDEANTTVTHGLLLAFG
                     IGALLNLLSLLIMKPYFRAFTNNEEIYQLSMAYMSVCSFMQIPNMVHITIQKMIQATG
                     NMVAPMWFQIAGVLVNFVFDPLLIFGIGIFPAMGIRGAAVATVAGYSLSMILAFALLL
                     GKKQKVRIKLKGFHFQKWLIGRVLTLGLPSFIMNALSSFMVTFVNFFLVAYSDTAIAF
                     FGAYFKVQQLIVMTVNGLIQGCLPIMRFNYGAGNRDRLHSAFRYGTALVLGMMILGTL
                     IVNFFPAQLLEMFTASKAMRSFGISAMRIMAASYLFCGLSTMISTYFQATEKVGSSMA
                     IQLCRQLLFFVPALWCLDKLFKLNGIWLAFPVAETATILIALVIMAWYHHKNIL"
     gene            6727..8061
                     /locus_tag="ACZPDN_06670"
     CDS             6727..8061
                     /locus_tag="ACZPDN_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298339.1"
                     /translation="MMNDTFMKEKPVLPLILSMSLPMVLSMLVNSLYNIIDSFFVAQI
                     SEEAMTALSLVYPVQNFINAVGIGFGVGINAVIAFYLGAGDNKKADQAATQGLVLAMI
                     HGFVLTVCCITMMPAFLGMFTSSKTVLELGIRYSFIAFGFTMFIIVGITFEKLFQAVG
                     NMKTTMISLMCGCITNIVLDPVLIFGYGPFPKMGIEGAALATGIGQALTLAIYLVVYF
                     VRSIRVNICRQYILLSKEMVIKLYSIGIPATLNLALASLLISALNAILAAYSEVYILV
                     LGIYYKLQTFIYLPANGIVQGMRPLIGYNYGAGENKRVSQIYKIVLCMSGIIMVLGTV
                     TCLLIPGQLIGLFTHTKATIHNGETALRIICAGFIVSAVSVTSSGALEGLGKGTPSLL
                     ISLCRYVVVIIPTAFLLSRLFGVVGVWNAFWITEVISAIISICVYYKAIAQS"
     gene            8308..>9034
                     /locus_tag="ACZPDN_06675"
     CDS             8308..>9034
                     /locus_tag="ACZPDN_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298340.1"
                     /translation="MVSRRRILWFAIGKMLRQDGWAEKYNPKNQFDVNQYDYSSCKEY
                     LAALKEKWQEYEDPECEFEDYVNVSNYSNYDDYAYDVDTYRTRLEWRDEMDRDCEFDV
                     NPCDFEYKEYYVKALKRAWKKELDPYDEFEYIDLELIDDVYDYRERIDECREWKDEHD
                     SNDEYNVDPSQFDDVEEYLDALRKLWKRKYDYFNEFSSIDPNDYSNEDDYSNAIENKK
                     NWMNKYDKNNAYKLDPSDYDCEED"
BASE COUNT         2827 a         1232 c         2091 g         2884 t
ORIGIN      
        1 gaagcatatt atgtggattc aataactcaa cagtcagata gtgaagtgtg gttttcatta
       61 aagaataaag atttaaacaa acaatatgtg tttaaatatg aaggtaatga attgattcca
      121 gacagattag tagagagtgt ggattaaatg gatatggatg ttattacaat actaaaattt
      181 gaagagatgg ttgttttata tattaagatt ttaaagaaaa caatcacgga cactgaactt
      241 gaattgttta agtataattt ggaaatttct tgttgtgtgc caaatgctat attttttaat
      301 ttaaatagtg aggcaaagaa gatattgggt aaaaaagagt ggattaaatt gtacagtcca
      361 gatattgaaa gagaatttca tagtggtcca atagaaacaa agagctagag tctttatgat
      421 tctagttttt ttattgactg aatcttgtga aataaaatat actattaaca aatggatatg
      481 gagaaatagt atgtttaaaa aagggaaaag gttagctgcg ttatgcacaa tcatttttgt
      541 ggttttatct atgtgcatgc cttgtttggc ggatgagacg cagaagacga ttcgtgttgg
      601 atcttttgaa gatacattta attatgtgga tgaacatggt gtaaggcgtg gttatggtta
      661 tgaattaatg caggctttgg ctgggtatac gggttggaaa tttgagtatg taaagtgtga
      721 ttggtcaaac tgttttgaaa agcttgaaaa tggtgagatt gatatcatgg gtgatattgc
      781 gtatacggat gatcgtgcta aagaaatgtt gttttctgag gaaacgatgg gtgaagaaaa
      841 gtatgttttg tatgcgaatt tatcggatat ggatattgtg tcttcggatt ttaagtcttt
      901 ggatggaaag cgtataggtg tgcttaaagg tacaaattct gaatatatgc ttacaaattg
      961 ggagaataag tatggtattc atactgagca tgtgaatgta agtggtaatg aggatgtaaa
     1021 aaagaaacta gcgaataatg agattgattg tttcgtgtct ttagaagagt caatttggtc
     1081 tgaaagaggg atttctagtg taacaacaat tggagagtct gatatatatt ttgtgattaa
     1141 taaggagcgt agtgatatta aacaagaatt ggattatgcg atgcgtcaat tggatagtga
     1201 tagtcctttt ttcaaggcgg atttgtataa gaagtatttt actttggatt atacgcaatt
     1261 tttaacaggt tttgaaaatt catatatcaa agagcatggt ggtattcgaa ttggtttttt
     1321 agataatgat tctgttgtat tttctatgga caaaaagagt ggagagattc gtggtacact
     1381 ttctgaatat atttcgtatg ccaaagattg tttaggaaat catactttaa aatttgatat
     1441 agaggctttt gatgattatg acaaaatgat tgaggcttta cagaataatg agattgatgt
     1501 gatttattat gcgagccgaa attcaaatgt tgccgaaaag aagggatatg cactttcgaa
     1561 tacggcttgg acgtataatt tgattgccgt taccaataag gaagatttta atgagaatga
     1621 tgcatatgtg gtagctgtac cgaaaaacaa ggtggcttta aagcaacata tcgcgtataa
     1681 cttatccgca ttggaaactc gtggattgtg attcgattga ggatgggacg aatatggttg
     1741 atgatggtaa agtagattgt tttatcatgg gatcaagtca ggtgttgagt tttgatggta
     1801 aaaagaattt taaaattatt cctttgacta agacaatgga ggcttgtttt gcggttaaag
     1861 atgggcaggc aacactatta tccatattga ataagacgat aaaggctatg ccttcggata
     1921 tgttgacaag tacaattgcg atgtatgata gtacggctta taaaacgact ttttacagtt
     1981 ttttaaaaga tcatttggtt gttgttcttg ttctaggttt tagtattatt attgtaattc
     2041 ttctactttt acaaaaggca agacgagcag aggcgagtgc taaggttgca ttaaagagtg
     2101 ctgaggatgc aagcttagct aagacacagt ttcttcataa tgtatcgcat gatattcgta
     2161 cgcctatgaa tgcgattttg ggctattctg aacttatgaa gaatgagttg aaggataatg
     2221 atatgcctag ggttttcgag catttaaata aacttcaaaa ggcggggaat attcttctat
     2281 caattattaa taacgtttta gatatggctc gcatagaaag tggtcatatg gaattggatg
     2341 aaaactatgc gaatattgag gatataagac aaagattgat tgagatgttt gaggatgagg
     2401 cgaagaaaaa gaatattcat gtgaggtaca cttttaatgt ggagcataaa catgtcatgg
     2461 ccgatgtgac taaggctgaa gaaatttttg tgaatatttt aagtaatgcc cttaagtata
     2521 caccttcaaa tggttctgtt gaagtgaatg tggatgagct ttcatgcgat gagcctggat
     2581 atatgattgt tcgtacaagc attatggata cgggtattgg tatgagtcag gagtatttgg
     2641 agaagatttt tgaatctttt tcaagagaaa gaatgacatc tagtaaaatt agtggtacag
     2701 gtcttggtat ggccattgtt aagaaatatg tggatcttct tggtggtaca attgatgtga
     2761 agagtgttct tggtaagggg agtacgttta ttgtcacgtt gaaatataaa ttggcggatg
     2821 aaaaatatta tttgaataaa caagaagaga attgtgaagt tgataatgca atttttaatg
     2881 gtaaacatat tcttttggct gaggataatg atttaaatgc cgagattgca caaaccatat
     2941 tagagcgtgc aggacttttt gtagatcgtg taaagaatgg tattgagtgt gtgaataggg
     3001 tcatagaaaa tccggttggt acatttgatg tcattttaat ggatattcaa atgcctaaaa
     3061 tggatggata taaagctacg tgtgaaatta gaaacttgca agataaggca aaagcatgca
     3121 tacctattgt ggcaataacg gccaatgcgt ttgaggaaga caagaaaaaa gcgatagatg
     3181 ttggtatgga tggtcatatc gcaaaaccaa ttaatattga agatttgttt gtggtattaa
     3241 cggatatttt gaataaacag aaaagctaga gtctatggat tctagttttt ttatgcaaaa
     3301 aagttaaatt ttttagaaaa accttatttt tttacgtata agagtatgta ggcaatctca
     3361 aatttagtat ctattgtacc tagttgatta gatagtcaaa tcattctttg tggtaaaaaa
     3421 acaaaagtgc aattgtgggt tcacttgttt attaaagcgg tcaagctata aaaaatacac
     3481 attacgatac ttatttatct agacgacagt taaatgccta ctagaaatga ggtgcttata
     3541 agaagtgcaa atttattaaa tgatttcgcc tttaagtatg tgtttggcga agattgtaaa
     3601 gaggccaatg atgccttaaa atcattactc acggtatttt tagaaaggag tgtgaataag
     3661 gttgttgtaa agaattcaga aatagtaaaa gactatagta aaatgaaaag tccaaggtta
     3721 gacttattag ttgaatttga tgatcaaacg atggtggatt tagaaatgca attaagacaa
     3781 acgaaagata atttgccaat tcgctttagt tattatttgg ctagacttca tggttctcaa
     3841 gaattggaag gaaaatatta tggagagtta aaggaaacaa ttgtcttagt gttctttaat
     3901 gtgaatttaa ttgataatca tagaatgtgc aacacattca cattgaaaaa tgaggatggc
     3961 ttatcttttg tgaaagaaac agaagatcgt atgaagatac gaacggttga aatggcaaag
     4021 ttggatataa ataagccgct tgaaaaaatg atttattact ttttgaattg ccacaaggga
     4081 atggatgata gtaaaattaa ggtaatgata gaaagtgatg gggtgattca aatgttagaa
     4141 aagcgtgtag aaaccatatc ggatgatggt tggaaaaaaa taatagaaga ttttcaaaaa
     4201 ttgcatgaaa atgaagaacg aatggaatta caacttgaaa ttgaagaagc aaataaacaa
     4261 gtagaagaag caaataaacg tgcagaagta gcaaataaac aagtagaaga agctaataaa
     4321 cgtgcagaag tagcaaataa acgtgcagat gaatcggata agaaaatgga tcagatgaat
     4381 caagtaatga aacagatgat tcaagcgatg ctaacaaatg ggatgagttt agagcttatt
     4441 tctaagtcat tgtcgaagag tgaagatgaa attatggagt tgttgaaata gtcatattct
     4501 aggaatttga aaaagtgaag atgataactg agtcgcctat aatatggcgg ctttttttgt
     4561 atacaatcaa agaaaaataa ttaggtatat ggttagtggt gtcacaattc cgtaatgatt
     4621 gatgcttgac agtctccttc aagtttgata taaatattaa tgcaaaattt tagtggactt
     4681 ttccggattc ggtcatcttt ttgccacaca gctttcttgt gatagttgtg tggctttttt
     4741 gttgcctgta tctttgaagt attaacaaaa tggaggacta tatctgtatg caaaaaattt
     4801 ttgtctctta ttaagacgcg gcgtagtgta caagcttata aatcggaacc tgtcccttcc
     4861 aaggctttgg acgctgtact ggaagcaggg acttgcgctc ctactggcgg aggatatcaa
     4921 tctccaacca tcattgcaat tacagattcc aaatatagga gaaagattac aaaattaaac
     4981 gcagaagtga tggtaggcaa cacagaccct tattatggcg cacctgtcgt gatcctagtt
     5041 ttggcagatg ggtccgcaag cacttatgtt gaggacggaa gttgcgtcct tgaaaatagg
     5101 atgcttgctg ctcatgcact tggcctggga accgtatgga ttcatcggga acttgagatt
     5161 tttgacagcg aaagtagtaa gaatctgttg cgggagtgga agctactaga aaccttgcgt
     5221 ggcatcggag cgattgcatt gggctatctg gctcaggaag ccggtaagcc ctcagcacgc
     5281 aaacaaaact atattgttcg gatttaatat ccctttgttt ggcacaattt ttttgcgccg
     5341 gacaatttgt tgagaggagg ggaagtatgc agactaataa taaaatggcg gttgctcctg
     5401 ttggaaggct catttggcaa atgtcgttac cgccgctaat ctccatgttt ctgcaatatt
     5461 cctataattt gatagacagt gcgtttatag cgcggttgag tgagaatgca ctagcggctg
     5521 tttctttgtc atttccaatt acaacactga tgaatgcggc gtccatctgg attggtgtcg
     5581 gcgtgaatgt actgattgca gggtatcttg ggcagaaaaa gcaggatgag gcaaatacca
     5641 ccgtcaccca tggattgtta ctggcctttg gaattggtgc tttgctcaat cttctgtcat
     5701 tgctgattat gaaaccctac ttcagggcat tcaccaataa tgaagaaatt tatcagttga
     5761 gtatggccta tatgagcgta tgttcgttta tgcaaattcc caacatggtg catatcacaa
     5821 tccagaagat gatacaggct accgggaaca tggtagctcc tatgtggttc cagattgcag
     5881 gagtactcgt taattttgta tttgacccac tcctaatttt tggtataggt atttttccag
     5941 ctatgggaat ccgtggcgct gcggtggcaa ctgttgcggg ttattcgtta tccatgattt
     6001 tggcatttgc cttgctactg ggcaaaaagc agaaagtgcg gataaagctc aaaggatttc
     6061 attttcaaaa gtggttgatt ggccgtgttt taacccttgg actgccatct tttattatga
     6121 atgcccttag ttcttttatg gtgacttttg taaacttttt tctggtggca tattccgata
     6181 cggcgattgc attcttcggt gcgtatttta aggtgcagca gctgatcgtt atgacagtaa
     6241 acggcttgat tcagggctgt ttgcctatta tgcggtttaa ctacggtgcg ggaaacagag
     6301 ataggctgca ttccgctttc cgatacggaa ccgctttggt ccttggtatg atgatactgg
     6361 gaactttgat tgtcaacttc tttccggcgc agcttttgga aatgtttaca gcatcaaaag
     6421 ctatgcgctc ctttggaata tccgctatgc gaattatggc ggcaagctat cttttttgcg
     6481 gcctctctac catgatttcc acctattttc aagcgacaga aaaagtaggt tctagcatgg
     6541 cgattcaatt atgcagacaa ctgcttttct ttgttcctgc cttatggtgt ctggacaaat
     6601 tattcaaact gaacggaatc tggcttgcat ttcctgttgc ggaaactgcc actatactca
     6661 ttgctctggt aataatggca tggtatcacc acaaaaatat cttataacat gttccaagga
     6721 ggagaaatga tgaacgatac ttttatgaaa gaaaaaccgg tattaccgct gattttatcc
     6781 atgtccctgc ctatggtttt atccatgctg gttaattctc tatacaacat tatagacagc
     6841 tttttcgtcg cgcagatcag cgaggaagct atgacggcat tatcactggt ttacccagtt
     6901 caaaatttta tcaatgctgt tggaattgga tttggcgttg gaatcaacgc agtgatcgcg
     6961 ttctatctag gtgctggaga taataaaaaa gcagatcaag ctgctacgca gggacttgtg
     7021 cttgccatga ttcacggctt tgttttgaca gtctgctgta ttacaatgat gccggctttt
     7081 ttaggtatgt tcacttcatc caagacagtg cttgaacttg gcatccgcta ttcttttatt
     7141 gcgttcggtt ttacaatgtt tatcatcgtg ggtattacat tcgagaaact attccaagct
     7201 gtaggaaaca tgaaaacaac catgatcagc ttgatgtgtg ggtgtataac aaacattgtc
     7261 ttagaccccg ttctgatttt tgggtatggt ccattcccta aaatgggaat cgaaggcgcc
     7321 gcgctggcaa ctggcatcgg gcaggccttg acgcttgcga tttatctggt cgtttatttt
     7381 gtgcggtcta ttcgcgtaaa tatctgcaga cagtacattt tgctcagcaa agagatggta
     7441 ataaaactgt actccatcgg cattcctgca accttgaatc ttgcactcgc atctcttttg
     7501 atttcggcgc tcaatgctat tttggcggca tattccgaag tgtatattct ggttttagga
     7561 atctattaca aattacagac attcatctat cttccggcta atggcattgt tcaggggatg
     7621 cgcccattga ttggctataa ctacggtgcg ggggagaaca aaagagtcag ccagatttat
     7681 aaaatcgttt tgtgcatgag cggtatcatt atggtgcttg ggacagtgac atgtctgcta
     7741 atccccggcc agcttattgg actgtttact cacacaaaag cgacaatcca taacggggaa
     7801 acagccctgc gtatcatctg tgccggtttt attgtctcgg cagtttctgt tacatcttcc
     7861 ggcgcactgg agggacttgg aaaaggcact ccttcattac tgatttcact ttgccggtat
     7921 gttgtggtta tcatcccaac tgcgttttta cttagcaggc ttttcggagt tgttggcgtc
     7981 tggaatgcgt tttggatcac ggaagtgatt tcagcaatca tttctatttg tgtttactac
     8041 aaggcaatag cacaaagcta gctgagctag tcaacagtaa aataaacgtg cagatgaatc
     8101 aattaatgaa actagggatt caagcgatgc taacaaatgg gatgagttta gagcttattt
     8161 ctaagtcatt gtcgaagagt gaagatgaaa ttacggagtt gttgaaatag tcatattcta
     8221 gaaatttgaa aaagtgaaga tgataactga gtcgcttata atatggcggc ttttttggtg
     8281 tataatcaaa gaaaaggaat taggtatatg gttagtagaa gaagaatatt atggtttgcg
     8341 attggtaaaa tgttgcgcca ggatggttgg gctgagaaat ataatcctaa gaatcaattt
     8401 gatgtgaatc aatatgatta ttcgagttgt aaagaatatt tggctgcact aaaagagaag
     8461 tggcaagaat atgaggaccc ggaatgtgaa tttgaggatt atgtgaatgt atcaaactat
     8521 tcgaattatg atgattatgc ttatgatgta gatacatata gaacaagatt agagtggcgt
     8581 gatgaaatgg atcgggattg tgagtttgat gtgaatccat gtgattttga atataaagag
     8641 tattacgtta aagctttaaa gagggcttgg aaaaaggaat tggatcctta tgatgagttt
     8701 gaatatattg atttagaatt gattgatgat gtgtatgatt atagggaaag aatagatgag
     8761 tgtagagagt ggaaggatga acatgattcg aatgatgaat ataatgtaga tccaagtcaa
     8821 tttgatgatg tagaagaata tttggatgca ttaagaaagt tatggaagcg taagtatgat
     8881 tattttaatg agtttagttc gatagatcca aatgattatt caaatgagga tgattattca
     8941 aacgcaattg aaaataaaaa gaattggatg aataaatatg ataagaataa tgcatataaa
     9001 ctagatccta gtgattatga ttgtgaagaa gatt
//
LOCUS       DBMDWL010000081         9001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2781_length_9001_cov_3.022472, whole genome shotgun sequence.
ACCESSION   DBMDWL010000081 DBMDWL010000000
VERSION     DBMDWL010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2781_length_9001_cov_3.022472"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="ACZPDN_06680"
     CDS             <1..1856
                     /locus_tag="ACZPDN_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR1298341.1"
                     /translation="IIDLSFIKEYPKEFGARVYKISSDDQGNRLTHVKITGGVLNAKD
                     KIGADEKVDQIRLYNGKQFEMVQTAYPGMICALKGLNNYEVGQGLGFESDMTKPLLNA
                     YMNYQLLLPEGVDALTMMRYCSVLAQEDPQLQIEYDEQTKQIFLRLMGSIQMEILQKE
                     IEKRSKVKVGFTTGKVLFKETIQNSVIGVGHFEPLRHYAEVHLKLEPLPRGKGLVFES
                     NCSNDDLALNWQNLILTHLKEKQHKGVLTGSPITDIKITLVAGKAHLKHTEGGDFRQA
                     TYRAIRQGLKEAESILLEPYYSFELIIENQYVSKALFDLENKHCTFKIEEDMNSLVHI
                     KGQGPVRKLMDYQKDVIAYTKGKGQFICELEDYHECFDQDKIVEESNYDSEADLNNPT
                     GSIFCEHGAGYFVPWDEVKEHMHLQIKEANSSSYTSYVKHTVHEEELKKVFNSLGGNN
                     KKAEKPIKKKTKVDLNLNQIHIEDKKPECLMIDGYNMIYDWQDLKDIAKVNIESARDE
                     LINRISNYQGYKRIKVILVFDGYRVKNNAGSHFNQGNLDIIYTKYNQTADSYIEKAVH
                     DLKKKYELSVATSDGLIQNAILANGAKRISARELEIAVKNVNKRALSYLKK"
     gene            1896..2819
                     /locus_tag="ACZPDN_06685"
     CDS             1896..2819
                     /locus_tag="ACZPDN_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1298342.1"
                     /translation="MTRKIAIIGAGPAGYTLAQELVKAENEYQIDIFDKEAEIGGAIY
                     TGIPEYRMPKTFLEKAYNGLVEAGVKFHFNTFVDQTMFKELQADYDYVVVAIGAQIEN
                     TFGFETGNGVVAGLTLLYDLNINHKQADFKKYKKAIVWGGGNVAMDCARSLVRIIDDV
                     TIVYRRSLQEMPASPAEIKACEKEGVKIAFLNNIKDILKDENGNVCGVQVAKMELGEM
                     DESGRASCHEVKDSLFVMDCDLVAMAIGQKVDFTPLNNDLKTTDTHASSLENVYIIGD
                     AFTGPKTIGSAVMEGKKIAKEIEAKYNEGTK"
     gene            complement(3201..4058)
                     /locus_tag="ACZPDN_06690"
     CDS             complement(3201..4058)
                     /locus_tag="ACZPDN_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1298343.1"
                     /translation="MRENIEKYIELIIGSILLSLGIYLFVTPNGINFGGAIGIAQIIE
                     YFIVKMVPSLGDMNLVGIINFMINIPLFVMGFKIMNKEFCIKTVISLVVQTITLSILP
                     KQSIVIMPDMLSNCIFGALMCGIGVGLALQSSGCCGGMDIAGVCLSKTKPGFSVGKLS
                     IIINSILFTICAFIFDLQTSLYSIIFVAILYFISDRIHYQNINMTVLIFTKKENMKNV
                     IMERTGRGVTYWMGKGAYTDTEQYILFCAVNKYEIRNFKKIVNEIDPKAFVTISEDQE
                     IDGGFEKRL"
     gene            complement(4072..4299)
                     /locus_tag="ACZPDN_06695"
     CDS             complement(4072..4299)
                     /locus_tag="ACZPDN_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298344.1"
                     /translation="MLHYYQNKNLEIEFLIEQNAKVIPVEVKAGNNRSQSMNQLLKNE
                     DIPYGYKFVNGNCGINGKKITFLLYLAMYVD"
     gene            4582..5748
                     /locus_tag="ACZPDN_06700"
     CDS             4582..5748
                     /locus_tag="ACZPDN_06700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR1298345.1"
                     /translation="MSKIFVHAKTMIDGVSDTPKFDQLITCEDGKITAIEPYHAINED
                     VVEANVVTPGFFNCHVHIMEPVGFGTDKEFTFVEKTFYTQKHLEDYINSGVTFIRVVG
                     TENDYDMDIRDCVDAGLVKGPHMLCAGRCICMTGGHGWQGGIEADGVDECRKVARTLL
                     KKGVDLIKVMATGGVMTKGVEPGSAQLTQEEMAAIIEEAHKAGRKTATHAQGTQGIKN
                     ALYAGIDSIEHGIFLDQECIDYMKEHGTYLVPTLVAPQCIVEKGVEAGIADYMVKKAI
                     YVKDAHVESFKKAYAQGVKIALGTDGGTPFNYHNNTAYEFELMEKYGVDRMDILKIAT
                     HNSADCLGVLDDYGTLEVGKNADLVCLKENPLDAISNVRKIDKVIKDGAVVVKK"
     gene            complement(5815..6129)
                     /locus_tag="ACZPDN_06705"
     CDS             complement(5815..6129)
                     /locus_tag="ACZPDN_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HIR1298346.1"
                     /translation="MNKREKEMRTVYEMIHLYCRKNHKEKELCPECLALYEYARMRIE
                     KCPFMETKTFCSQCKVHCYKKDKREQIRKVMRFSGPRMLVYHPVMAICHLYYQMKDSV
                     GS"
     gene            complement(6129..6317)
                     /locus_tag="ACZPDN_06710"
     CDS             complement(6129..6317)
                     /locus_tag="ACZPDN_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR1298347.1"
                     /translation="MHDSKLVLLDEPTSNLDSLNEGVILKSIYEERKNKTIIFVSHRE
                     STLSHCDRVIHMESNRVS"
     gene            complement(6562..>7173)
                     /locus_tag="ACZPDN_06715"
     CDS             complement(6562..>7173)
                     /locus_tag="ACZPDN_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family accessory protein
                     TnpB-related protein"
                     /protein_id="HIR1298348.1"
                     /translation="HKYKDELYARVNEKHNSPDKAVAYELMDYGEYFIVKAIFEKHMN
                     LPKTDTLYGAIGIDINVDHIALCETNMDGNIVLIKKYPIHKENTKNKRNEELYQMAIE
                     IMEQCKSKKKSLVVEDLNFKQLKTRMLYCPKKQNKTLSSFAYKKILEKLERKCLMNEV
                     DVIKVDPRNTSKIGKEKYTKIKGLSVHYCAAYVINRRGMGFVD"
     assembly_gap    7174..7183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7184..7831)
                     /locus_tag="ACZPDN_06720"
     CDS             complement(<7184..7831)
                     /locus_tag="ACZPDN_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298349.1"
                     /translation="MKVFSMSQRIYYKDLEPDAESIIKKDLELYNCMLHKAFKICFDR
                     AYKDVTYSDTDQRMIKSFYGTSDYFPLSAINEAKALVKSLKCLEKENQDMIKTRLKKI
                     NKKIKKNEKQLKKALKEKEKLINRSKKKKYTEEDYLYEVQVLDPNIKRLKSIIRNLKF
                     RRNRNEFKLKRKMPSVCFGGKKNLKNGYLDTYRFKRARRMLIPGRRQGKYSNNLFK"
     gene            8046..8219
                     /locus_tag="ACZPDN_06725"
     CDS             8046..8219
                     /locus_tag="ACZPDN_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298350.1"
                     /translation="MDTTYHSNIPIETLSLLDCDVRSFDFNHPIKNIFFDNIEYIRKL
                     DASGKARPCILTM"
     gene            8201..8782
                     /locus_tag="ACZPDN_06730"
     CDS             8201..8782
                     /locus_tag="ACZPDN_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025677.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HIR1298351.1"
                     /translation="MHFDNVERSLLCHTCYLGFDQFECTDCDNWNIIPHSCHSRFCNA
                     CGVKYTKQLAAKATSFCLDCPHRHIVFTIPEELRNWFRQDRTRLNILFVAARNTICIL
                     VNRSLLKKLKMKQLKNTHYIFKDIPVRNEFGMIATLHNFGRDLKWNPHIHCLVPKLVY
                     SLKTNKLKMFHHFNFSRLRKTFDEFDLSRKLAD"
     gene            8782..>9001
                     /locus_tag="ACZPDN_06735"
     CDS             8782..>9001
                     /locus_tag="ACZPDN_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298352.1"
                     /translation="MKPEEKNRLYKDHPKGFYVYAKFKSTDNDSNNNKDIQGCVNFFL
                     VTLEDVSWLKIESQNTIKNQKLSVVSTMI"
BASE COUNT         3004 a         1553 c         1476 g         2958 t
ORIGIN      
        1 cgatcattga tttatcattt atcaaagaat atccaaaaga atttggtgct cgtgtatata
       61 aaatatcaag tgatgaccaa ggcaatcgct taactcatgt aaaaatcaca ggtggtgtat
      121 taaatgccaa agataaaatt ggcgcagatg aaaaagtaga tcagattcgt ttgtataatg
      181 gaaaacaatt tgaaatggtg caaaccgcct atccaggtat gatttgtgct ttaaaaggat
      241 taaacaacta tgaagtaggc caaggtttag gctttgaatc cgacatgaca aaaccacttt
      301 taaatgccta catgaactat caattattac ttcctgaagg tgtagatgca cttactatga
      361 tgcgctattg tagcgtactt gctcaagaag atccacagct tcaaatcgaa tatgatgaac
      421 aaacgaaaca aattttcttg cgtttgatgg gatccattca aatggagatt cttcaaaaag
      481 aaattgagaa aagatctaaa gtgaaggttg gttttacaac gggtaaagtt ttatttaaag
      541 aaaccattca aaatagtgtg attggtgtag ggcactttga acctttaaga cactatgccg
      601 aagtccattt aaaacttgaa ccattaccta gaggtaaagg ccttgtattt gaatccaatt
      661 gttccaatga tgatttggcc ttaaattggc aaaatctgat tttaacgcat ttaaaagaga
      721 aacaacacaa aggtgtatta actggatcgc caattaccga tataaaaatc actttagtgg
      781 ccggtaaagc tcatttaaaa catacagaag gtggagactt tagacaagca acctatcgtg
      841 ctataaggca aggtttaaaa gaggctgagt ctatattatt agagccgtat tatagttttg
      901 aactgattat agaaaatcaa tatgtctcaa aagcattatt tgatttagaa aataagcatt
      961 gcaccttcaa aattgaagag gatatgaaca gccttgtcca cattaaaggt caaggccctg
     1021 tgcgtaaatt aatggattat caaaaagatg tcattgcgta tacaaaaggt aaaggtcaat
     1081 ttatttgtga attagaagat tatcacgagt gttttgatca agataaaatc gtcgaagaat
     1141 ctaattatga cagtgaagct gatttaaaca atccaaccgg atctattttc tgtgaacatg
     1201 gagctggata ttttgttcct tgggacgaag tcaaagaaca tatgcaccta caaattaaag
     1261 aagccaatag ttcttcttat acttcttatg tgaagcatac agttcatgaa gaggagttaa
     1321 agaaagtatt taactcgctt ggtggaaaca acaagaaagc tgaaaagcca atcaagaaaa
     1381 aaacaaaagt agatttaaat ttaaatcaga ttcatattga agataaaaag ccagaatgct
     1441 taatgattga tggatacaat atgatttatg attggcaaga tttaaaagat attgccaaag
     1501 tcaatattga atcggctcgc gatgaattga ttaatcgaat ctcgaactat caaggctaca
     1561 aacgaatcaa agtgatttta gtatttgatg gatatcgcgt taaaaataat gcgggatctc
     1621 attttaatca aggaaatcta gatattattt atacaaaata caatcagaca gccgatagtt
     1681 atattgaaaa agctgtacat gatttgaaaa agaaatatga attatccgtt gcgacaagtg
     1741 atggtttaat tcaaaatgcg attcttgcga atggtgcaaa aagaatctcg gctcgcgaac
     1801 ttgagattgc cgtaaaaaac gtaaataaac gtgcactttc atacctaaag aagtagttta
     1861 tgatataata gcgaaaaaga tggaggaatt taattatgac aagaaagatt gccattatcg
     1921 gagccggccc tgctggctat acattagctc aagagcttgt taaagcagaa aatgagtatc
     1981 aaatcgacat tttcgataag gaagctgaaa taggtggagc catttatact ggtattcctg
     2041 aatatcgtat gcccaaaaca ttcttagaaa aagcctacaa tggactcgtt gaagctggtg
     2101 ttaaattcca ttttaatact tttgtagatc aaactatgtt taaagaacta caagcagact
     2161 atgattatgt ggttgtagcc attggtgctc aaattgaaaa tacttttggt tttgaaacag
     2221 gcaatggtgt cgttgctgga ttaacgctac tttatgattt aaacatcaac cacaaacaag
     2281 cagactttaa aaaatataaa aaggctattg tttggggtgg aggaaatgtc gcaatggatt
     2341 gtgcaagatc tttagttcgt attattgatg atgtaactat cgtataccgt agatctttac
     2401 aagaaatgcc tgcaagccct gcagaaatca aagcctgtga aaaagaaggc gttaagattg
     2461 ctttcttaaa caacatcaaa gacatcttaa aagatgaaaa tggaaatgtt tgtggtgttc
     2521 aagttgcgaa aatggaactt ggagaaatgg atgaatctgg gagagcaagc tgtcatgaag
     2581 taaaagactc actatttgta atggattgcg atctagttgc gatggctatt ggtcaaaaag
     2641 tagattttac accattgaat aacgacctaa aaacaaccga tacacacgca tcttcattag
     2701 agaatgtata cattattgga gatgccttta cagggccaaa aacaattggt agtgctgtaa
     2761 tggaaggtaa aaagattgcc aaagaaatcg aggcaaaata caacgaagga acgaaataat
     2821 aaaaatcgtt cctttttttg tgttttatgt ttttttcaca ttgaccacct aaaataataa
     2881 ttggaacata caagcagcaa gcatatgtat acttctggga atggatttat cattgcattt
     2941 taatgacaat cccagagtgc gtataaaaac ctctggtatc attaaaaggt ggtagtcatg
     3001 aacaattacg aattgtaagt cctaattgta tctgtattag acatttacac aactctaatt
     3061 ggctttgtga ttcagctttt gactgaatga atcaaacaca aaatgaataa acaaaataaa
     3121 aactacctac gccaactcta gcaaagtttg taggtagttt ttactacaac taattccaga
     3181 aacatatttg tatgtctata tcacaaacgc ttttcaaatc caccatcaat ttcttgatcc
     3241 tcgctaattg ttacgaatgc ttttggatca atttcattga ctattttttt gaagttacga
     3301 atctcatact tatttacagc acaaaataaa atatactgtt ctgtatctgt atacgcacct
     3361 ttacccatcc aatacgttac accacggcca gttctttcca tgatcacatt tttcatgttc
     3421 tctttctttg tgaaaatcaa tacagtcatg ttgatatttt gataatggat acgatcacta
     3481 ataaaataaa gtatagccac aaaaataata gaatagagtg atgtctgtaa atcaaaaata
     3541 aaggcacaaa ttgtaaataa gatcgaattg ataataattg atagcttacc tacggaaaaa
     3601 cctggttttg tcttcgataa acaaacgcct gcaatatcca tacctccaca acatccactg
     3661 ctctgtaagg caagtcctac tccaatgcca cacatcaaag ctccaaaaat acaatttgaa
     3721 agcatgtctg gcataatcac aatagattgt tttggcaaaa tagatagagt aatggtttgc
     3781 accactaaac taattacagt cttgatacaa aactctttat tcatgatttt aaaccccatc
     3841 acaaacaatg gaatattgat cataaagtta ataataccca ccaagttcat atcaccaaga
     3901 cttggcacca tctttacaat aaaatactca ataatctgtg caataccaat ggcaccacca
     3961 aagttgattc cattaggtgt cacaaacaaa taaatcccta acgaaagtaa gatacttcct
     4021 ataattaact caatatactt ttcaatattt tctctcattt tttacctcag cctaatccac
     4081 atacatagct aagtataata gaaaagttat ttttttacca ttaattccac aatttccatt
     4141 cacaaattta tatccataag gaatatcttc atttttcaac aattgattca ttgattgcga
     4201 acgattattt cctgccttta cttcgactgg aattactttt gcgttttgtt caatcaaaaa
     4261 ttctatttct agatttttat tttggtaata gtgaagcaaa tgtggaacat cccgcaagcg
     4321 caaatgccat acacatgctc actacacttc attttaccat tactttccct ccaaatattt
     4381 gtgtgcgatc cattatgcat cattttcatg ttgtttcatg tattttataa atgttttcat
     4441 ttgttgttag tattagttaa taccacactt tatataaagt attttttaat atgtaagtaa
     4501 ttattttatt ttctgaaagc acttgcattt attgcttgtt tagagcataa tactatgtaa
     4561 attgagaaaa ggagtatttt tatgagtaaa atttttgtac atgctaaaac aatgatagac
     4621 ggtgtgagtg atacacctaa atttgatcaa ttgatcactt gtgaagacgg aaaaatcaca
     4681 gctattgaac cttatcatgc aatcaatgaa gatgtagtcg aggcaaatgt tgtaacgcca
     4741 ggcttcttta actgtcacgt tcatattatg gaacctgttg gctttggaac cgataaagaa
     4801 ttcacttttg ttgaaaaaac gttctacaca caaaaacact tagaagatta tatcaacagt
     4861 ggtgttactt ttattcgtgt tgtaggaacc gaaaacgatt acgatatgga tattcgtgat
     4921 tgtgtggatg caggtcttgt taaaggacca cacatgttat gtgctggacg ttgcatctgt
     4981 atgacaggag gccatggctg gcaaggtggt attgaagccg atggtgtcga tgaatgtaga
     5041 aaagtggcta gaactctact taaaaaaggc gtcgatttaa ttaaagttat ggcaacaggt
     5101 ggcgttatga caaaaggtgt agaacctgga agtgctcaat taacacaaga agaaatggca
     5161 gccattattg aagaggctca taaagcagga agaaaaacgg ctactcacgc acaaggaacg
     5221 caaggtatca aaaatgcatt atatgccggc attgattcca ttgaacatgg aattttctta
     5281 gatcaagaat gcattgatta catgaaagaa cacggtacat atttggttcc tacattagtt
     5341 gcccctcaat gcatcgttga aaaaggtgta gaagctggta ttgccgatta catggtcaaa
     5401 aaagctattt atgttaaaga tgctcatgtt gaaagcttta aaaaagctta tgcacaaggc
     5461 gttaaaattg ctttgggtac agatggtgga actccattca actaccacaa caacacagcc
     5521 tatgaatttg aattaatgga aaaatacggt gtagatcgta tggatatttt aaaaattgcg
     5581 acacacaatt cagcagactg cttaggtgtt ttagatgatt atggcacact tgaggttggc
     5641 aaaaatgcag atttagtttg tttaaaagaa aatcctttgg atgcaatttc aaacgttcga
     5701 aagattgata aagtcattaa agatggtgct gttgtagtta aaaaataatc atatttactt
     5761 tgtttaggga tgattcgatt catccctttt ttcaatagaa aagggccata tccactatga
     5821 cccaacacta tctttcattt gataatacaa atgacaaatt gccatgacag gatgatatac
     5881 taacattcta ggacctgaaa atcgcattac tttacgaatc tgttcccttt tatccttctt
     5941 gtaacaatgc accttgcatt gcgaacaaaa cgtctttgtt tccatgaacg gacatttttc
     6001 aatacgcatt cttgcgtatt catacaaagc caaacattct ggacacaatt ctttttcttt
     6061 atgatttttt ctacaataca aatgaatcat ttcgtacact gtacgcattt ctttttcacg
     6121 tttattcatt agctaactcg attcgattcc atatgaatca cacgatcaca atgcgatagc
     6181 gttgattcac gatgtgacac aaaaattatg gtcttatttt ttctttcttc atagatcgat
     6241 tttaagataa ccccttcatt caagctatcc aaattacttg taggctcatc caataataca
     6301 agtttagaat catgcaagaa aagactttcc aaaggaaagt cacacgtcta aatgacgtgc
     6361 ccggttcgtt tccagtcgtc cggtaacatt tcaatatcca ctccatcttt ctgaatcatt
     6421 cggtatcccg attcattcag tcttttgcga tggagttttt attgtatgct ttttatagat
     6481 tcttcagaat ttcatccatg atctatgtat ctatcatcca gtaagatatc ataaacttct
     6541 ggttctgatt atttatctta tttaatccac aaatcccata cctcttcgat tgatcacata
     6601 ggctgcacaa taatgtacac tcaatccttt gatctttgta tacttttctt ttccaatctt
     6661 actcgtattt cttggatcta ctttgattac atccacttca ttcatcaaac attttctttc
     6721 taatttttct aatatctttt tatacgcaaa actcgataat gttttatttt gttttttagg
     6781 gcaatagagc atccttgttt tcaactgctt gaagttcaag tcttcaacta ctagactctt
     6841 cttcttggat ttacattgtt ccatgatttc aatggccatc tgatacagtt cttcatttcg
     6901 tttgttcttg gtattctctt tatggattgg atatttcttg atcagaacga tatttccatc
     6961 catattagtt tcacatagtg ctatatggtc cacattgata tcgattccaa ttgctccata
     7021 caatgtatct gtctttggaa gattcatatg tttttcaaat atagctttca caataaaata
     7081 ttcaccataa tccatcaact cataggctac agccttatcg ggtgaattgt gtttttcatt
     7141 gactcttgcg tacaattcat ccttgtattt atgnnnnnnn nnntttgaaa aggttgtttg
     7201 aatacttgcc ctgtcttctt ccaggaatca acattcttct ggctctttta aaacgatagg
     7261 tatctaagta accattttta agattctttt taccgccaaa acatacagaa ggcatctttc
     7321 ttttaagttt gaactcatta cggtttcttc tgaattttag attgcgaatg atactcttca
     7381 atcttttgat atttggatct aagacctgta cttcatacaa atagtcttct tctgtatatt
     7441 tctttttctt ggatctgttg atcagtttct ctttttcctt taaagccttc ttcaattgct
     7501 tctcgttctt tttgattttc ttgtttatct ttttaagacg cgtcttgatc atatcttgat
     7561 tttctttttc aagacattta agactcttca ccaaagcctt ggcctcatta atggcagaca
     7621 aaggaaaata atcacttgta ccatagaagg acttgatcat tctttgatcc gtatctgaat
     7681 aggtcacatc cttataggca cgatcaaaac agatcttgaa tgccttatga agcatacagt
     7741 tatacagttc aagatccttt ttaatgatgg attctgcatc tggttccaga tctttatagt
     7801 agattctttg tgacattgaa aacaccttca taagacaaat cctcctgttc tatctgactc
     7861 tattatacta tatttaatac aggattaaca gatagatttg agactttctg atttatttta
     7921 tttatatcat tatatcatca atgtgtatat gtgatatttt actgtattct cacatgtcca
     7981 cattaaattc acattggaac tgtattttta ttcaatttaa aatggtttca ttctatactt
     8041 caattatgga cactacttat cattcaaaca ttccaattga aactctatca ttattagatt
     8101 gtgatgttcg aagctttgat ttcaatcacc ctattaaaaa catcttcttc gataatattg
     8161 aatacattcg aaaattagat gcttccggta aagctcgtcc ttgcattttg acaatgtaga
     8221 acgttctctt ctttgccaca catgttatct tggatttgat cagtttgaat gtacagattg
     8281 tgacaactgg aatattattc cacattcctg tcattcaaga ttctgtaatg cctgtggtgt
     8341 taaatatacc aaacagcttg cagccaaagc aacttctttt tgtcttgact gccctcatag
     8401 acacattgtc tttactattc ctgaagaact tagaaactgg ttcagacaag atcgtaccag
     8461 actcaatata ttgtttgttg cagctagaaa tacaatatgt atactagtga acagatcatt
     8521 attaaagaaa ctgaagatga aacaattaaa gaatactcat tatatattca aagatatccc
     8581 tgttcgtaat gaatttggta tgattgccac tctacataat tttggaaggg accttaaatg
     8641 gaatccacac atccattgcc ttgtaccgaa actcgtatat tcactaaaaa caaacaaact
     8701 aaagatgttt caccatttca atttctctag gcttagaaaa acatttgatg aattcgactt
     8761 atccaggaag ttggcggatt aatgaaaccc gaagaaaaga acagactcta taaagatcat
     8821 cctaaagggt tctatgtcta tgccaagttc aaatctactg acaacgattc caacaataat
     8881 aaagatattc aaggctgcgt aaactttttt ctcgttacgc tggaagatgt gtcatggctg
     8941 aaaatagaat cacagaatac aataaagaat caaaaattgt cagttgtttc tacaatgatc
     9001 a
//
LOCUS       DBMDWL010000082         8859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2841_length_8859_cov_2.608244, whole genome shotgun sequence.
ACCESSION   DBMDWL010000082 DBMDWL010000000
VERSION     DBMDWL010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2841_length_8859_cov_2.608244"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            245..1309
                     /locus_tag="ACZPDN_06740"
     CDS             245..1309
                     /locus_tag="ACZPDN_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR1298353.1"
                     /translation="MRNFDYSHLKDRTWDNEILSYVAKIHEFKGKQELYFRQKPVELE
                     RLVEIAKVQSTESSNRIEGIVTTRSRLQQLVEDKTTPRNRNEKEILGYRNVLNLIHEN
                     YQYIPLKSNYILQLHRDLLQYTELSYGGKYKTSPNEIDAVLPNGEKRILFKPLEPYET
                     PDAIEQICSSYQQALDHEIVDSLILIPCVILDFLCVHPFNDGNGRMSRLLTLLLLYRN
                     DYVVGKYISIEKEIANTKEAYYQALAQADLGWHEGTNDPEPFIKYMLGIILKCYREFE
                     ERLTISEKSGKRSSSYDIVKTYVNGTIGTFTKRDAMENCPSLGSSSVELALKKLVEDG
                     TIIRKGLGRASHYIRKDAIE"
     gene            complement(1456..1677)
                     /locus_tag="ACZPDN_06745"
     CDS             complement(1456..1677)
                     /locus_tag="ACZPDN_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1298354.1"
                     /translation="MGKIILRLDRVMADRKMSLKELSEKVGVSNVNLSKMKTGKISAI
                     RFSTLEAICEVLNCQPGDILEYVKDDENS"
     gene            complement(1680..2276)
                     /locus_tag="ACZPDN_06750"
     CDS             complement(1680..2276)
                     /locus_tag="ACZPDN_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298355.1"
                     /translation="MNDKSKDLLKKMCFILEIIRGTCLISVVLLIGLSLFYAFNKDVY
                     NTLFGSIRIDYLQLIFNDDLALNKDKMSMWLPLILLITAIFVFIIYKAVQTIESICTI
                     AMDHTPFDVRVSNHIKNLAKYILVGGIVFNILEVCRIMQFKQTINFNLLFNTKYVTQI
                     NFDIRLHLSFLVFAALIYLLSYIFRYGQELQQLSDETL"
     gene            2499..2651
                     /locus_tag="ACZPDN_06755"
     CDS             2499..2651
                     /locus_tag="ACZPDN_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298356.1"
                     /translation="MIAIELNVHAHSIAVSLIEVIIATLYATACCPNIVYTVLEMSKG
                     IAKGMP"
     gene            complement(2669..4417)
                     /locus_tag="ACZPDN_06760"
     CDS             complement(2669..4417)
                     /locus_tag="ACZPDN_06760"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1298357.1"
                     /translation="MYLENINGPSDIQKLNLDELRILADETRDALIHKISLAGGHNGP
                     NLGMVEVTTALHYVFHSPIDKFVFDVSHQSYPHKILTGRKQAFLDEEHFHDVTGYTNP
                     EESEHDFFTIGHTSTSISLALGLAKGRDLNHGKENIIAIIGDGSLSGGEALEALDYAG
                     EYRNNLIIIVNDNDQSIAENHGGLYKNLKELRESNGNCTNNIFESFGLEYHYLEDGHD
                     LFKLVDLFNSVKDIDHPVVLHIHTIKGKGLAYAEKNREAWHAGGPFHVEDGSPRFTCG
                     SNDTTVFDSIKTLLDNNEKAIVLNAGTPMGLGFTQDVRQEYVDRGQFIDVGIAEENMV
                     AMSSGITKNGGTAVFGTYAPFLQRTYDQLSHDLCLNDNPAVMLVLSPGVYGMNSNTHI
                     ALCDIQMFAHISNLIYLAPATKEEYEQMFKYATTQKDHPVGIRIPASFVSSGIKDETD
                     YSVLKNKVEIQGEKFAIFAVGCMLALAKKVAEKIKEEIGIQITVINPRFLTGLDIELL
                     EKLKKNHTHILTLEDGELVSGYGQNIASYYGDSDIKVKNYGISKVFHTDYDPIELLAK
                     NGMSVENLSQYIQENI"
     gene            complement(4503..5717)
                     /locus_tag="ACZPDN_06765"
     CDS             complement(4503..5717)
                     /locus_tag="ACZPDN_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1298358.1"
                     /translation="MIKRELYMSRIRPFIGTDLIKVMTGIRRCGKSVMLKLIQQELTE
                     SGINPKQFISINFEDMNYSHLQTAQALHDEISAKASEIGGKVYLFFDEIQEVKDWEKC
                     INSFRVSLDCDIYITGSNANLLSSELATYLGGRYVEFVIYPFSFKEFMELYHSITPDA
                     SIQQCFQKYLIVGGMPYLSNIRYADAPSKQYLQDLFNSVQLKDIMKRNKIRDIDLLER
                     IIAYVIANVGTTFSASSLAKFLKNEKRTVSPETILNYIKYCCEAYLFYQVKREDLQGK
                     QILSSNEKYYIADHGIREAIFGGNMRDINLILENIVYLELLRRGFDVTVGRNGNKEID
                     FVCNKQGEKIYVQVTYLLASQETIDREFGAYDRIKDNFPKYVVSLDEFDMSQNGIKHR
                     NIRDFLLAKEWN"
     gene            complement(5828..6064)
                     /locus_tag="ACZPDN_06770"
     CDS             complement(5828..6064)
                     /locus_tag="ACZPDN_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298359.1"
                     /translation="MTSDALRDLLYNELRFGVRTLIFVANFFLLPRVCLALKDYRNLM
                     PDTIGLIVFDNTEWTNFSSPTVHVVLMGIFIFIP"
     gene            complement(6069..6203)
                     /locus_tag="ACZPDN_06775"
     CDS             complement(6069..6203)
                     /locus_tag="ACZPDN_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298360.1"
                     /translation="MTESILNLGTRLIDDGFDEFIMITADPHVLSRNKRKNMQNTRCS
                     "
     gene            complement(6266..6931)
                     /locus_tag="ACZPDN_06780"
     CDS             complement(6266..6931)
                     /locus_tag="ACZPDN_06780"
                     /inference="COORDINATES: protein motif:HMM:NF026158.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM164 family acyltransferase"
                     /protein_id="HIR1298361.1"
                     /translation="MRKKEDFTLQNACYFFMFICLLSETTKMMSDMIPSTHGGMHLNP
                     QSLPFHICSILLFIAAYITFGKDGPLKQKMINFFAVVGTCGGIAAILIPTYGVEFNVA
                     NVYQCFMYHATLTWFSLYLIRFKHANLGMQAFKNNLVVLFALLIFNLYMNSILAVYDT
                     NFMFIVRPPLEGLPFLNLNHGYYIYLFRVIMLGVIGLSLFHLPFIVKERKSCETESDF
                     NIA"
     gene            7185..8033
                     /gene="nudC"
                     /locus_tag="ACZPDN_06785"
     CDS             7185..8033
                     /gene="nudC"
                     /locus_tag="ACZPDN_06785"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR1298362.1"
                     /translation="MVQDIYPHKLHNEFDPNVQATQEDVLLCIVNGKILIHLNCFENK
                     KVVFPKVSDIQVENVEYRYLFSIDNTKYFLCDMQLGEDQIPSGYGYVEERSLRIEGIG
                     PKDNLFAAITGKHLADWYRDTKFCGRCGHKMVHSTTERAMVCPECHNTVYPRIMPAVI
                     VGVINGDKLLLTKYRTGFKYNALIAGFTEIGETVEETVKREVMEEAGIKVNNIRYYKS
                     QPWGSANDILLGFFCNVKGNDEISMDTNELKYADWVQRDEIILQPGEFSLTNEMMKLF
                     KENKVE"
     gene            8123..8680
                     /gene="efp"
                     /locus_tag="ACZPDN_06790"
     CDS             8123..8680
                     /gene="efp"
                     /locus_tag="ACZPDN_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1298363.1"
                     /translation="MAISAGDFKTGLTLIVDGDPWVVLDFQHVKPGKGAAILKTKMKN
                     LKTGNTQERNFNASTKFDQANISKKAAQFSYEADNTFYFMDMETYDTYELSEEQVGYN
                     KYFIVEGTEVYLVFFEGLLLNVSVSEKVELTIVETDPAIKGAPSNQTKDAKTETGLTL
                     RVPQFIEQGEKIVVFSADGKYAGRA"
     gene            8698..>8859
                     /locus_tag="ACZPDN_06795"
     CDS             8698..>8859
                     /locus_tag="ACZPDN_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1298364.1"
                     /translation="MGKVVLITGGAKGIGRAIALELAKNQYDIVINYLTSNQEAECLK
                     EHIIQQYHVR"
BASE COUNT         3042 a         1368 c         1533 g         2916 t
ORIGIN      
        1 tatttaccaa attgattttt tatcgtatat acaagtaatg gattcaaaat gaatacagaa
       61 tatacataaa atcgagttca aataatttag ttaatatcaa aaaagatata ctaggtttac
      121 attaaaagtt ttaaaggtcg atttatatat tttgattcat gttttttact cccagcttat
      181 tctttttctc ccaaaatcag tgtataattg ggagaaagag tattctcttt gggaggagaa
      241 ggaaatgcgg aattttgatt atagtcatct taaagatcgt acatgggata atgaaatatt
      301 gtcttatgtt gctaagattc atgaatttaa aggtaaacaa gaattatatt ttcgacaaaa
      361 gcctgtagaa ttagagcgtt tggttgaaat agctaaagta caaagtacag aaagttcaaa
      421 ccgtattgaa gggattgtaa ccactagatc ccgtcttcaa caattggttg aggataagac
      481 aacgcctaga aatcgcaacg aaaaagaaat cttaggatat cggaatgttt taaatttaat
      541 tcatgaaaat tatcaatata ttccgttaaa gtcaaattat atattgcagc tgcatcgaga
      601 tttgcttcaa tacacagaat tatcctatgg tggaaaatac aaaacgtctc caaatgaaat
      661 agatgctgtt ttgccaaatg gtgaaaaaag aatattattc aaaccattag aaccttatga
      721 aacaccggat gccattgaac aaatttgtag tagctatcaa caagctttag atcatgaaat
      781 tgtagattca ctgatattaa ttccctgtgt aatattggac tttctttgtg ttcatccttt
      841 taatgatgga aatggcagaa tgagtcgatt actaacgttg ctccttttat atcgtaatga
      901 ttatgtagtt ggtaaatata tcagtattga aaaagaaata gcgaatacga aagaagcgta
      961 ttatcaggct ttagcacaag cagatttagg ttggcatgaa ggaacaaatg atcccgagcc
     1021 atttattaaa tatatgcttg gcattatttt aaaatgttat cgtgaatttg aagagagatt
     1081 aacaattagt gagaaaagcg gaaaaagaag tagctcatat gatattgtta aaacatatgt
     1141 aaatggcacg ataggtacct ttacgaaaag agatgctatg gaaaattgcc cgagtttagg
     1201 cagttcttct gtggaattgg cactaaaaaa attagttgaa gatggtacta ttattcgtaa
     1261 aggtctaggc cgcgcatcgc attatattag aaaagatgcg attgaataat tattatatac
     1321 taaaaatata tgacgaatct tttcttatat tcagtgtgca tgaacagaca ataatgggat
     1381 taactgaatt tgaaatatag tatgtagtgt tattgaaatt taaaatcata ataaaatagc
     1441 tagaagtttg tgcttctagc tattttcatc gtcctttaca tactccaata tatcaccagg
     1501 ttgacaattt aatacttcac aaatagcttc taaagtagag aagcgaatcg cagaaatctt
     1561 acctgttttc atttttgata agttgacatt gctgacgcca actttttcag acaactcttt
     1621 taaactcatt tttcgatcgg ccatgacacg atctaatcgc aatatgatct tacccataat
     1681 tacaatgtct catcactcaa ttgttgtagt tcttgtccat atctaaatat ataagacaat
     1741 aaatagatta gagctgcaaa aacgaggaag cttaagtgaa gacgaatatc aaaattgatt
     1801 tgtgtaacat atttagtgtt gaaaagtaga ttgaagttga tggtttgttt aaattgcata
     1861 atacgacata cttcaagaat attaaagaca ataccaccta caagaatata cttggcaagg
     1921 ttttttatgt ggttagaaac acgaacatca aatggagtat gatccatcgc aatcgtacaa
     1981 atggattcaa ttgtttgtac agctttatat ataataaata caaatatagc ggtgattaat
     2041 aaaatcaatg gtaaccacat ggacatttta tctttattta atgccaaatc atcattaaaa
     2101 atcaattgaa gatagtcaat tcttatgctt ccaaatagcg tattatatac atccttgttg
     2161 aaggcataaa ataagcttag tccaattaac aacacaacag aaattaaaca agtccctcga
     2221 atgatttcta aaataaaaca cattttcttt aataaatctt tagacttatc attcatgata
     2281 ttacctccta acattaaata attaatgttt atcattaatt ttattatata ttacataaat
     2341 aataagtcaa tatgatttac tttaaaacgc caatttcttt taaaaataat tcacgatctt
     2401 ttcgaatcaa gaagacactt gtgatataca tgcctggatc attgataggg cgaataatca
     2461 tatccatggt atgtcgtgtc tccgcatcag aaaaagggat gattgcgatt gagttaaatg
     2521 tacatgccca ctcaattgcg gtttcgttga ttgaagtaat aatcgcaaca ttatatgcaa
     2581 cggcatgttg tccaaatata gtatataccg tgttggaaat gtcaaaagga atcgctaaag
     2641 ggatgccttg aaattttgat gttcggtctt aaatattctc ttgtatatat tgagacagat
     2701 tttcaacaga cattccattt ttagctaata attcaattgg atcatagtct gtgtggaaaa
     2761 cttttgagat accataattc tttactttta tatcactatc tccataataa gatgcgatat
     2821 tttggccata tccagatacg agttctccat cttctaaagt tagaatgtgc gtatgatttt
     2881 tctttaactt ttctaataac tctatatcta atccagttaa aaatctagga ttgatgactg
     2941 taatttgaat gccaatttct tcttttattt tttctgcaac tttttttgct aaagctaaca
     3001 tacatccaac cgcaaagatt gcgaattttt ctccttggat ttcaacttta tttttaagta
     3061 cagaataatc tgtttcatct ttgattccac tagaaacaaa acttgcggga atacgaatac
     3121 caaccggatg atccttttgt gtagtcgcgt acttaaacat ttgttcatat tcttcttttg
     3181 tagctggtgc taaataaata agatttgaga tgtgcgcaaa catttggatg tcgcataggg
     3241 cgatatgcgt attggaattc atgccataga caccaggact caacacaagc attacagctg
     3301 gattatcatt taaacataaa tcgtgtgaaa gctggtcata ggttctttga aggaatgggg
     3361 cataggttcc aaatacggcg gttcctccat ttttggtaat accgctactc attgcgacca
     3421 tgttttcttc ggcgatacct acatcgataa attgtccacg atctacatat tcttgacgaa
     3481 catcttgtgt aaaaccaagt cccattggtg ttcctgcatt taatacaatg gctttttcgt
     3541 tgttgtctag aagtgtttta atgctgtcaa atacagtcgt gtcattgcta ccacaagtaa
     3601 aacgaggact accatcttcc acatggaatg gaccacctgc atgccatgct tctctatttt
     3661 tttcggcata agccaaacct tttcccttaa tggtatgaat atgtagtaca acaggatgat
     3721 caatatcttt tacagaattg aataaatcca ccaatttaaa taggtcatgt ccatcctcta
     3781 aataatggta ttccaaacca aaactttcaa agatgttgtt tgtgcaattt ccattggatt
     3841 cacgaagttc ctttaaattt ttatataaac caccatggtt ttcagcaata ctttgatcat
     3901 tatcattcac aataataatt aaattgtttc tatattctcc tgcatagtcc aaggcttcta
     3961 aggcttcgcc accagaaaga gaaccatctc cgataatcgc aatgatgttt tcttttccat
     4021 gatttaaatc tcttcctttt gccaaaccca aagctaaaga aatcgatgta gatgtgtgtc
     4081 caatcgtaaa gaaatcgtgt tcactttctt ctgggtttgt atatccagtt acatcatgaa
     4141 agtgttcttc atccaaaaaa gcttgttttc gtccagttag gattttatgt ggataagact
     4201 gatgagaaac gtcaaagacg aatttatcaa ttggcgaatg aaaaacatag tgtaatgcag
     4261 tggttacttc gaccatacct aagttaggac cattatgtcc tccggcaagg gaaattttgt
     4321 ggataagtgc atcacgtgtt tcatctgcca aaatacgtaa ttcatctaga tttaattttt
     4381 gaatgtcact tggaccatta atattttcta aatacatgtc attacctcct gtaattgaag
     4441 gtatgatagc acctaaagtt aactttaggt caagcgttta atgaaatatt ttgccatttt
     4501 gtttagttcc attccttggc tagtaggaaa tcacggatgt ttcgatgctt gatgccattt
     4561 tggctcatgt caaattcatc aagagaaaca acgtactttg gaaaattatc tttaatacga
     4621 tcataagcac cgaattcacg atcaattgtt tcttgggatg ccaatagata agtgacttga
     4681 acatatattt tttcgccttg tttgttgcat acaaaatcta tttctttatt gccatttctt
     4741 cctacagtta catcaaatcc tcggcgcaat aattctagat atacgatatt ttctaaaata
     4801 agattgatgt ctcgcatatt accaccgaaa atagcctcac ggatgccgtg atcggcaata
     4861 taatattttt cattggaaga aagaatttgt ttaccttgta aatcttctcg tttcacttga
     4921 taaaacaagt aagcttcaca acagtatttg atatagttca aaattgtttc cggagataca
     4981 gttcgctttt cgttttttaa aaactttgcg agtgaggatg cagaaaaagt tgttcccaca
     5041 ttcgcaatca cataagcaat gattcgttct aataaatcaa tatcgcgaat tttatttcgt
     5101 ttcatgatgt ctttgagttg aacagagtta aatagatctt gaagatattg tttagacgga
     5161 gcatctgcat aacgaatgtt tgaaagataa ggcatacctc cgacaataag gtatttttga
     5221 aaacattgct gaatggatgc atcaggagta atcgaatgat acagttccat gaattcttta
     5281 aaagaaaatg gatatatcac aaattctaca taacgaccac ctaaatacgt tgcaagttca
     5341 ctagatagta gatttgcgtt tgagcctgta atgtaaatgt cacaatccaa cgatacgcga
     5401 aaagagttga tacatttttc ccaatctttg acttcttgta tttcatcaaa gaatagatac
     5461 acttttccac ctatttctga ggcttttgca ctgatttcat catgcagtgc ttgtgcagtt
     5521 tgtaagtggg agtagttcat atcttcaaaa ttgatagaga taaactgttt tgggttgata
     5581 ccagactctg taagctcttg ttgaattagc tttaacatga cggatttgcc acagcggcga
     5641 atcccagtca tgactttgat taagtcagtt cctatgaatg gacggatgcg actcatatat
     5701 agttcacgtt tgatcataat ttaaagcctc ctttaattat agtttaacac atttataagt
     5761 gatgaatagt gttaaaaata taaaatcata agttataact gatgaaaaat gaaaatgcac
     5821 gtaatgttta aggtataaaa ataaaaatac ccataagtac aacatgtacg gtgggacttg
     5881 aaaagtttgt ccattctgta ttgtcaaaga caattaatcc aattgtatct ggcattaggt
     5941 tacgataatc ttttaatgcc aaacaaactc gtggtaataa gaagaagttg gctacaaaga
     6001 ttagagtacg aacaccaaaa cgtagttcat tatatagtag atcacgtaat gcatcgcttg
     6061 tcacatcatc agctacaacg tgtgttttgc atgtttttcc ttttgtttct acttaataca
     6121 tgaggatctg ccgtaatcat aatgaattca tcaaatccat catcaattaa gcgtgttcct
     6181 agatttaata tagattcagt cattgtttca taataaagtt ctcctaaata aaatggagtt
     6241 ttcttggtat aagaaaagct gtggactaag cgatattgaa gtcgctttct gtctcacagc
     6301 tttttctttc ttttactatg aatggtaaat ggaatagact aagtccaatc actccaagca
     6361 tgatgactct aaataaatag atatagtatc catgatttag gttaaggaat ggaagtcctt
     6421 ctaatggtgg tcttacaata aacataaagt ttgtatcata aactgccaaa atagaattca
     6481 tatataaatt aaaaattaat aacgcaaata atacgactaa attgttttta aatgcctgca
     6541 tacctaagtt cgcatgtttg aaacgaatca aatatagaga aaaccatgtt aatgttgcat
     6601 gatacatgaa acattgatat acattggcta cattaaattc aacgccatac gttggaatca
     6661 agatagcggc gattcctcca caagtaccga caaccgcaaa gaagttgatc attttttgtt
     6721 ttagtggacc atcctttcca aaagtgatgt aggccgcaat aaacaataag atactgcaga
     6781 tatggaatgg aagggattgc ggatttaagt gcattcctcc atgtgtactt ggaatcatat
     6841 ctgacatcat ttttgtggtt tcgctaagta aacatataaa cataaagaag taacaagcgt
     6901 tttgtagcgt gaaatcttct ttttttcgca tagtgattca taattaggat aaatagaata
     6961 caaattccta tccatataaa gtgttttgta gtaaacatat gccctctccc tgtagtcaac
     7021 agattatatc attcaaaaca taagtaaaca atgacaaaat ccttaaactt atctaaaata
     7081 ttcttaaatt acatgatata cttgattttt agatgataaa aaggatgtcg tatccttgtg
     7141 attgcaggaa tacgatgtat aaatttacaa gggggcatca gaaaatggta caagatattt
     7201 atccacataa attacacaat gaatttgatc ctaatgtaca ggcaacccaa gaggatgtat
     7261 tgttgtgtat tgtgaatgga aagattttaa ttcatttaaa ttgttttgag aataagaaag
     7321 ttgtttttcc taaagtgagt gacatacaag ttgaaaatgt agagtatcgc tatttgtttt
     7381 caattgataa tacaaaatat ttcttatgtg atatgcaatt aggagaggac cagattccat
     7441 caggatatgg gtatgttgaa gagagaagtt tgcgtattga agggattggt ccaaaagaca
     7501 atttgtttgc ggccattact ggaaaacatt tggcagattg gtatcgagat actaaattct
     7561 gcggaagatg tggacacaag atggtgcatt caacaacaga aagagctatg gtttgtccag
     7621 agtgtcacaa tacagtctat ccaagaatta tgccggctgt tattgtaggt gtaatcaatg
     7681 gggataagtt attgctgaca aaatatcgta cgggatttaa gtataatgcg ttgattgctg
     7741 ggtttactga aattggtgaa accgtagaag aaacggtaaa acgtgaagtt atggaagagg
     7801 caggaatcaa agtcaacaat atacgttact ataagtcaca gccttgggga agtgccaatg
     7861 acattttgtt ggggttcttc tgtaatgtaa aaggaaatga tgaaatatct atggatacca
     7921 atgaattgaa atatgcggat tgggttcaaa gagatgaaat tattcttcag ccaggtgaat
     7981 ttagtttaac gaatgaaatg atgaaattat ttaaggaaaa taaagtagag tgaaagtatc
     8041 acactcattc ttctaaaaat gatgaggata tggtacaata aagcgtgtaa aaaatattta
     8101 tagaaaatag gagtgaaaaa ttatggcaat ttctgcaggt gattttaaaa caggattaac
     8161 tttaatcgtt gatggtgatc cttgggtagt attagatttc caacatgtta aacctggtaa
     8221 aggtgcagct atcttaaaaa ctaagatgaa aaacttaaaa acagggaata cacaagaaag
     8281 aaacttcaat gcttcaacta aatttgatca agctaatatt tctaaaaaag cagctcaatt
     8341 ttcttatgaa gcagacaata ctttctactt catggatatg gaaacatatg atacatatga
     8401 attatctgaa gaacaagttg gttataataa atactttatt gtcgaaggaa ctgaagttta
     8461 tttagtattc tttgaaggat tattattaaa cgtttctgtt tctgaaaaag tagaattaac
     8521 tattgtagaa acagatccag ctatcaaagg ggcaccatct aaccaaacaa aagacgctaa
     8581 aactgaaaca ggtttaactc ttagagttcc tcaatttatt gaacaaggtg aaaaaatcgt
     8641 tgttttttca gctgatggaa aatacgcagg aagagcttaa taaagctctt ttttatcatg
     8701 ggtaaagttg tattaattac aggtggggcc aaaggtattg gtagagccat tgctttagag
     8761 ttagcgaaga atcaatatga tattgttatt aattatttaa cgtcaaatca agaagcagaa
     8821 tgtttaaaag aacatattat tcaacaatat catgtacgt
//
LOCUS       DBMDWL010000083         8749 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2897_length_8749_cov_3.049607, whole genome shotgun sequence.
ACCESSION   DBMDWL010000083 DBMDWL010000000
VERSION     DBMDWL010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2897_length_8749_cov_3.049607"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="ACZPDN_06800"
     CDS             complement(<1..602)
                     /locus_tag="ACZPDN_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1298365.1"
                     /translation="MKHIVTGILAHVDAGKTTCIESMLYTSKTIRKLGRVDHQDAYLD
                     YDSQERERGITIYSKEAHFRWKNSEIYLIDTPGHVDFSSEMERSLQVLDLAILLINGQ
                     DGIQSHTSTIWKCLKHYNIPTLIFINKMDISYKTKEELMNDLKTHFSSNCIDFKSTDA
                     EDELSMLNEDIMNHYLETETIDTSLLQQAIFKRECFPVFFG"
     gene            666..1283
                     /locus_tag="ACZPDN_06805"
     CDS             666..1283
                     /locus_tag="ACZPDN_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1298366.1"
                     /translation="MKVLMLNGSPHPSGVNDLALCEMEAVFKENGIETEIVQVGNVAV
                     RGCMACGACTNLGKCVFDDIVNEIAPKFEKADGIVVSSPVYYASANATLIAVLDRLFY
                     STCFDKTMKVGASVVSCRRGGASATFDELNKYFTISGMPVVSSQYWNSIHGNNALEAN
                     QDEEGKQTMRTLARNMIFLMKSIALGKEAYGLPEKEVRKGTNFIR"
     gene            1294..1884
                     /locus_tag="ACZPDN_06810"
     CDS             1294..1884
                     /locus_tag="ACZPDN_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298367.1"
                     /translation="MVNLRHSDNIFYDREGDLFVITTDLDGRQEVVCKTNLECVSDVI
                     ARLLDINFKKVCDSENRLNMLGDLEQFACVYALKLMTYKTVKGLYLEFPTLAKYQGTA
                     LISPHTVIDNRNDGYNNKYHLMLVNVIYTSLIHATVKYAGSSMEAEHMKDVFETVLPD
                     NKGLIETLNDFYDGAINEADLTDIIKNIQSDNTTKA"
     gene            1971..2588
                     /locus_tag="ACZPDN_06815"
     CDS             1971..2588
                     /locus_tag="ACZPDN_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298368.1"
                     /translation="MKQIIQYIEDHNISEEEVAQAAHMSLRNLRRQIHSKNRTQVRIV
                     LLLADKQNQSIDSIFFDEMNNQPISLEGLTINQIQDIIKLVHPELFMGVTRSKKIKDY
                     NYNLETDMGDRMRFIREVVFSLSQTEFGKYMEVSRNTSKYWDEGQINVDKIFKISQST
                     NISMDFIIRDHYPLTLQTHGMSEALYLAVMTSCVLYRLRNAGSVK"
     gene            2715..3248
                     /locus_tag="ACZPDN_06820"
     CDS             2715..3248
                     /locus_tag="ACZPDN_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1298369.1"
                     /translation="MKIGKKLKELRTQNGLTLEELANRSELTKGFLSQLERDLTSPNI
                     SALENILEALGTNLADFFQSSKEEQIVFHTQDFFVNEQEDFVTEYIVPNAQKNQMEPI
                     LLTLKPGAKSQEVKAHEGEEFGYVLKGSIVLVVGNKRLKVKSKETFYITGKEGHYLEN
                     VSSADAKVLWLSTPPVF"
     gene            3269..4852
                     /locus_tag="ACZPDN_06825"
     CDS             3269..4852
                     /locus_tag="ACZPDN_06825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298370.1"
                     /translation="MEQEKNKLIQFRHIVKEFDGQVVLKGIDLDIYENEFVTLLGPSG
                     CGKTTLLRILGGFLEPSEGQVILNGEDICEVPAYKRELNTVFQKYALFPHMNVYDNIA
                     FGLKIKKMSKDVIDQKVMKMLKLIGLEGFENKDVTLLSGGQQQRVAIARALVNEPSVL
                     LLDEPLSALDLKLRKEMQYELKKIQQEVGITFIFVTHDQEEALTMSDKIVIMKDGEIQ
                     QVGSPEDIYNEPVNQYVAKFIGESNIIPARMICDKKVRFDDITFDCVDVGYKENEPVD
                     VVIRPEDIDIVDVKDGKMTGEVESVLFKGVHYEIMVETVPGTHVTVNMHVNKNYAITS
                     EDGKEKISANDFYLDLEDMKDIDDKEIIARADAQAWNPETDEFISIHDIDTDLKQEVG
                     EYTVTFSTNNKTSITRKIWVVDQRVVENKKANEAVSAFNFFKTVDEIKESMAIDTDLK
                     TWANAQGWKLDDENETVDLDVDYDFDPETIEEGIYKVTFWTTGREFKIHTTDYVEEGK
                     EVGLTFFAEDIHVMEKMGF"
     gene            4856..5683
                     /locus_tag="ACZPDN_06830"
     CDS             4856..5683
                     /locus_tag="ACZPDN_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1298371.1"
                     /translation="MKKFQQLAIPYIVWACIMLLLPMLLIAFYSVIQQGNSIVHFKLT
                     LDNYVRFFTDKDFLLILWRSLWIAIKTTIICLLIGYPIAYFISKNSPKIQQILVLAIT
                     LPTWINMLVRTYAWIGILSSDGIISSFLGLFGIHTELLYTEAAVLIGMVYNFLPFMIL
                     QINTALTKMDKSLLEAAQDLGANKFQTFWKVIFPLSLPGVVSGITLVFLPAVSSFFIP
                     KLLGGGQFFLIGNVIENQFITVGEWNFGSAISMIMAMIMMLLMMLVRKLEERNKEEK"
     gene            5686..7572
                     /locus_tag="ACZPDN_06835"
     CDS             5686..7572
                     /locus_tag="ACZPDN_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1298372.1"
                     /translation="MSKDKRVLGKVGMALLLVFFYAPILFMIIFSLNSSKSLTHFTGF
                     SLCWYEAMLKNHGMMESLYVTIVIALLATIISTIIGTITAIGLSKSKKVLRTFISQVN
                     DFPIMNPEIVTAIGLMLLFITFQIEKGFVTLLLAHIAFCIPYVILSIMPKIKSLDPNL
                     ADAAMDLGATPWQALVKVIVPQIMPGIVSGALIAFTMSFDDFVISYFVTGQGVKNLSI
                     MVYTMSKRINPSVNAISTLVVLLITITLTIVNVLPMIRKKTSPSKQNKPWKWAVAGVV
                     VVGLVASLFGLNKSASTLPYAGQTLHVYNWGEYTGENIISGFEELTGAKVIMDNFDSN
                     EQMYIKVANGDAYDVLVPSDYMIQRMMQEDMLQKLEPETRKECLSELMEAIKGLPYDP
                     KNEYSIPYFWGTVGIVYDKTKVSEEDLENEGWNIFLDQKYKGDIYLYDSERDSFMMAL
                     KALGYSMNTTSADELNVAFNWLVQCVQTMDPEIVTDEIIDNMAQARKALGLIYSGDAA
                     YVMSENENMGFYMPTSGTNLWSDAMVIPKNAKNPKLANEFIRYITSYDAAMDNSSYVG
                     YTSPNKEVTEELGGKGGDYDGINAYTPRSGYDKDEVFQYDEATRKIIADLWSRVKVAA
                     SNAH"
     gene            7623..8648
                     /gene="eutH"
                     /locus_tag="ACZPDN_06840"
     CDS             7623..8648
                     /gene="eutH"
                     /locus_tag="ACZPDN_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HIR1298373.1"
                     /translation="MNPIIFILLCFAALGLFDKMFKNRLGLATSFDRGIITMGDFMMS
                     VGGFYCIAIAFLNGHATLFKNKEMIISSLLAPDLGGYSIVESMTHADNILIFCGVLLT
                     STLGCLISFQLPIFLNELDTDDLSHYLKGVVYGILGLLPILIGCGFLLHIDHFLIVFL
                     PVIFICAILTGLFFISFQTLIVVLTLFSKLVQFVGYIFFFLVCLTFFFNMNFTNATLI
                     NEALRIVFQMSIIVCGSLVFCEIILRKFSDQIERVGQILNIDKYSVMGIILSFGTSIA
                     MLPLFSKMNRKGKILNAAFSLSGAFVFGGQLGFIASVNPASVTWFVVAKLLAGILGLV
                     IANVLEE"
BASE COUNT         2821 a         1159 c         1806 g         2963 t
ORIGIN      
        1 ccaaagaata cagggaagca ttctcttttg aagatggctt gttgaagaag tgaagtatca
       61 attgtttctg tttccagata atgattcatg atatcttcat tcaacataga taattcatct
      121 tcagcatctg tggatttaaa atcaatgcag ttggatgaaa aatgtgtttt tagatcattc
      181 atgagctctt cttttgtctt ataactaata tccattttat tgataaaaat caatgtagga
      241 atgttatagt gttttaagca tttccaaatc gttgaagtat gagattgaat accatcttgt
      301 ccattgatca agagaatggc taaatctaat acttgaagac ttctttccat ttcgcttgaa
      361 aaatcgacat ggcctggtgt atctatcagg tagatttcac tatttttcca acgaaaatga
      421 gcttcctttg aataaattgt aatacctctt tctcgttctt gactatcata atctagataa
      481 gcatcttgat gatcaacacg tcctaatttt cgaattgttt ttgatgtata aagcatactt
      541 tcgatacatg tggtttttcc agcatcgaca tgtgctaata ttccagttac tatatgtttc
      601 ataaaaacta ttttatcatg ttgacgcaaa tgataaaata gagatagttt ttttcggagg
      661 aaaatatgaa agtcttaatg ttgaatggaa gtccacatcc aagtggagta aatgatttgg
      721 ctttatgtga aatggaagct gtatttaaag aaaatggtat tgaaactgaa attgtacaag
      781 ttggaaatgt agctgttcgt ggttgtatgg cttgtggtgc atgcacgaat ttaggtaaat
      841 gcgtatttga tgatattgtg aatgaaattg cccctaaatt tgaaaaggca gatggaattg
      901 ttgtttcaag tccagtatat tatgccagtg cgaatgcgac attgattgcc gtattagatc
      961 gtctattcta tagtacttgt tttgataaga caatgaaagt tggcgcaagt gttgtatctt
     1021 gtcgaagagg aggggctagt gctacttttg atgaattaaa taaatatttc acgattagtg
     1081 gtatgcccgt tgtatcaagt caatattgga attcgattca tggaaataat gcgcttgaag
     1141 ccaatcaaga tgaagaggga aaacagacaa tgcgtacttt agctcgtaat atgattttct
     1201 taatgaaaag tattgcctta ggtaaagagg catatggttt gcctgaaaaa gaagtaagaa
     1261 agggtacgaa ttttattcgt taaaggtgat tgtatggtga atttaagaca ttcagataat
     1321 atattttatg atcgagaagg cgatttattt gtgatcacaa ccgatctaga tggtcgtcaa
     1381 gaagtggttt gtaagaccaa tctagaatgt gtgagtgatg taattgctcg attgttagat
     1441 attaacttta aaaaggtttg tgacagtgag aatcgtttaa atatgttagg tgatttagaa
     1501 caatttgcgt gtgtatatgc attaaaatta atgacctata aaacagtaaa aggtttgtat
     1561 ttagagtttc ctactttagc taaatatcaa ggtacagctc taattagtcc acacacagtt
     1621 attgataatc gtaatgatgg ttataataat aaatatcatt taatgttagt gaatgtgatt
     1681 tatacatctt tgattcatgc gactgttaag tatgcaggtt caagtatgga agctgagcat
     1741 atgaaagatg tatttgaaac ggttttacca gataataaag gattgattga aactttaaat
     1801 gatttctatg atggagctat caatgaagcg gatctaacgg atattatcaa gaatattcaa
     1861 agtgataata caactaaggc ttaaaaaaca ttttcatcga ctgattttta aaatatggtc
     1921 gagtacaaac aaagcaaaca tctctatgtt aaacatgggg gtgttttttt atgaaacaaa
     1981 ttattcaata tattgaagat cataatatat ctgaagaaga agttgcccaa gcagcccata
     2041 tgtctttgcg taatttaaga aggcagattc actcaaaaaa tcgtacacaa gtaaggattg
     2101 tcttgcttct tgcagataag caaaaccagt cgattgattc cattttcttt gatgaaatga
     2161 ataatcagcc aattagttta gaaggattaa cgataaatca gattcaagat attataaaat
     2221 tagtacatcc cgaactattt atgggtgtta cgcgaagtaa aaaaattaaa gattataatt
     2281 ataatttaga aaccgatatg ggtgatcgca tgcgctttat acgtgaagtt gtgttttctc
     2341 tttcacaaac tgaattcggt aagtatatgg aagtgtcaag gaatacatca aagtattggg
     2401 atgaaggaca aatcaatgtg gataagatct ttaaaatatc gcaaagtaca aatattagta
     2461 tggactttat catacgcgat cattatccct taacattgca gacgcatggt atgagtgaag
     2521 ccctttattt agctgttatg acgagttgtg tgttgtatcg tttgcgcaat gcaggaagtg
     2581 ttaaatagtt ttaacttagg agcaagaaaa attgctcttt tttttcttgc ataattgaag
     2641 gtttcctata taatagattt gttcttttaa tgaaacaaaa agtttagtat aaaaaacaaa
     2701 ttaggggtga ttgaatgaaa ataggcaaaa aactaaaaga gcttcgaacc cagaatgggt
     2761 tgactttgga agaacttgcg aatcgtagtg aattaacaaa aggcttttta tcccaattag
     2821 agcgcgattt aacaagtcca aatatttctg cattagaaaa tattttggaa gctctaggaa
     2881 cgaatttagc ggatttcttt cagtcgagta aagaagaaca aattgttttt catactcagg
     2941 atttttttgt gaatgaacaa gaagattttg ttacagaata tattgttcca aatgcacaaa
     3001 agaatcaaat ggaaccaatt ctattgactt tgaagccagg ggcaaaatca caagaagtca
     3061 aggcgcatga aggtgaagaa tttggctatg ttttaaaggg tagtattgtt ttagttgttg
     3121 gtaataaaag gttaaaagtt aaatcaaaag aaaccttcta tattacaggt aaggaagggc
     3181 attatttaga aaacgtatcg agtgcggatg ccaaagtatt gtggctatct acaccacctg
     3241 tattttaatg gacaaggaga gagtatagat ggaacaggaa aagaataaat taattcaatt
     3301 tcgtcacatt gtgaaagaat ttgatggaca agttgtatta aagggtattg atttagatat
     3361 atatgaaaat gagtttgtca ctctgttagg gcctagtgga tgtggaaaaa caactttatt
     3421 acgtatttta ggtggttttt tagaaccaag tgaaggacaa gtcatcttaa atggagaaga
     3481 tatctgtgag gttccggctt ataagcgtga gttgaataca gttttccaaa aatatgcatt
     3541 attccctcat atgaatgtat atgacaatat tgcgtttggt ttaaaaatca agaagatgag
     3601 taaggatgtc attgatcaaa aagttatgaa aatgttgaag ctgattggtc ttgaaggttt
     3661 tgaaaataaa gatgttactt tactatctgg tggtcagcag caacgtgttg cgattgcgcg
     3721 tgccctagtt aatgagccaa gcgtattatt gttagatgag ccacttagtg cactagattt
     3781 gaaacttcgt aaagagatgc agtatgaatt gaaaaagatt caacaagaag taggaatcac
     3841 atttattttc gttacacatg atcaagaaga agcgctgaca atgtcagata agatcgttat
     3901 tatgaaagat ggcgagattc aacaggttgg tagtccagaa gatatttata atgagcctgt
     3961 caatcagtat gttgccaagt tcattgggga aagtaatatt attccagctc gtatgatttg
     4021 cgataaaaaa gtacgttttg atgacattac atttgattgt gtggatgttg gatataaaga
     4081 aaatgaacca gtggatgttg tgattcgtcc agaagatatt gatattgtgg atgtaaaaga
     4141 tggcaagatg actggggaag ttgaaagtgt attatttaag ggtgttcact atgaaattat
     4201 ggtggaaaca gttcctggta cgcatgttac ggtaaatatg cacgtaaata agaactatgc
     4261 gatcacaagt gaggatggaa aagaaaaaat ttctgccaat gacttctatc tagacttaga
     4321 agatatgaaa gatattgatg ataaggaaat cattgcgcgt gccgatgcac aagcctggaa
     4381 tccagaaacg gatgaattca tttctattca tgatattgat acagatttaa aacaagaagt
     4441 tggagaatat actgttactt tctcaacaaa caacaagact tcgatcactc gtaagatttg
     4501 ggttgtagat caaagagttg ttgaaaacaa gaaggcaaat gaagccgtat ccgcatttaa
     4561 cttctttaaa acagtcgatg aaatcaagga atctatggct attgatacag atttgaagac
     4621 ttgggcgaat gcgcaaggtt ggaagttgga tgatgaaaac gaaacagtgg atctagatgt
     4681 agattatgat tttgatcctg aaacaatcga ggaaggaatt tataaggtta ctttctggac
     4741 aacaggacgt gaatttaaga ttcatacaac ggattatgta gaggaaggaa aagaagtcgg
     4801 acttactttc tttgcagaag atattcatgt tatggagaaa atgggattct agaaaatgaa
     4861 aaaatttcaa caattagcaa tcccttatat tgtgtgggct tgtattatgt tgttgcttcc
     4921 gatgttgctg attgcgtttt attcagtcat tcagcaaggc aacagtattg tgcactttaa
     4981 gctaacattg gataactatg ttcgtttctt tacagacaaa gatttcttgt tgattttatg
     5041 gcgctcttta tggattgcca ttaagacaac cattatttgt ttgttgattg gttatcctat
     5101 cgcatatttt atttcaaaaa atagtcctaa gattcaacaa atcttagttc ttgcgattac
     5161 acttcctact tggatcaata tgcttgtaag aacatacgca tggattggaa ttttatcaag
     5221 cgatggaatt attagtagct ttttaggttt gtttggaatt cacactgaac ttttatatac
     5281 agaagctgcc gtgttgattg gtatggtata taacttcttg ccatttatga ttttacagat
     5341 caatacagct ttgacgaaga tggataaatc tttattagaa gctgcacagg atttgggggc
     5401 caataaattt caaactttct ggaaagttat tttcccactt tctttacctg gtgttgtttc
     5461 aggtattaca cttgtattct tacctgccgt aagctcattt ttcattccta aattattggg
     5521 tggaggacaa ttcttcttga ttggtaacgt gattgaaaat caattcatta ccgttggaga
     5581 atggaacttt ggttctgcaa tctcaatgat catggctatg atcatgatgc ttctaatgat
     5641 gcttgtaaga aaattggaag agcgtaataa ggaggaaaaa tagacatgtc gaaagataag
     5701 agagtattag gtaaagtagg catggcttta ttattggtgt tcttttatgc accaatatta
     5761 ttcatgatta ttttttccct caacagttca aaatcattaa cacattttac aggattctca
     5821 ttatgttggt atgaggctat gcttaaaaac catggcatga tggaatcttt atatgtcaca
     5881 attgtgattg ccttattggc aacgattatt tctacaatca ttggtacgat tacggcgatt
     5941 ggtttatcaa aatcaaagaa agtattgcgt acttttataa gtcaggtcaa tgattttcca
     6001 atcatgaacc ctgaaattgt tacagccatt ggattgatgc ttttatttat cacattccaa
     6061 attgaaaagg gatttgtgac attattactt gcgcacatcg ctttctgtat tccatatgtg
     6121 attttgagta ttatgccaaa aattaaatca ttagatccaa acttggcaga tgccgcaatg
     6181 gatcttggag ctacaccttg gcaggcttta gtaaaagtca ttgtgccaca aattatgcca
     6241 ggtattgttt caggtgcttt aattgcgttt acaatgtctt ttgatgattt cgttatttca
     6301 tattttgtaa caggtcaagg cgtgaaaaac ttgagtatta tggtgtatac aatgtctaag
     6361 cgtattaatc caagtgtaaa tgcgattagt acattagtcg tattgttgat cacaatcaca
     6421 ttaacaattg tgaatgtttt gccaatgatt cgaaaaaaga cgagtccttc aaaacaaaat
     6481 aaaccttgga aatgggcagt tgcaggtgtt gtagtcgttg gcttagtggc ttctttattt
     6541 ggtttaaata aatcagcaag tactttacct tatgcaggac agactttaca tgtatataac
     6601 tggggtgaat atactggaga aaatattatt tctggttttg aagaattaac aggtgccaaa
     6661 gttattatgg ataactttga ttcaaacgaa caaatgtata tcaaggttgc caatggggat
     6721 gcatatgatg tattggttcc tagtgattat atgattcaaa gaatgatgca ggaagatatg
     6781 cttcaaaaat tagaaccaga aacacgtaaa gagtgcttaa gtgaattgat ggaagcaatc
     6841 aagggacttc cttatgatcc aaagaatgaa tattcaattc cttatttctg gggtactgtt
     6901 ggtattgtgt atgataagac aaaagtatcg gaagaggact tggaaaatga aggttggaat
     6961 attttcttag atcagaaata taaaggggat atttatctat atgattctga aagagattca
     7021 tttatgatgg cattaaaagc acttggatat tcaatgaata cgacaagtgc ggatgaattg
     7081 aatgttgcgt ttaactggtt agtacaatgc gttcagacaa tggatccaga aattgttacg
     7141 gatgaaatca ttgataatat ggcacaggct agaaaagctc ttggacttat ttattctgga
     7201 gatgcggctt atgtcatgtc agaaaatgaa aatatgggat tctatatgcc aacaagtgga
     7261 acaaaccttt ggtcagatgc gatggttatt cctaaaaatg cgaagaatcc taagcttgcg
     7321 aatgagttta ttcgttatat tacaagttat gatgcagcca tggataattc aagttatgtt
     7381 ggatatactt caccaaataa agaagttacg gaagaacttg gtggcaaagg tggagattat
     7441 gatggtatta acgcatatac acctcgttca ggttatgaca aggatgaagt gttccaatat
     7501 gatgaagcca cacgtaagat cattgcggat ttgtggtcta gagtaaaagt tgcagcaagt
     7561 aatgcacatt agtcgaagaa aatttcttcg actttttttc tttcttataa tagaatgaag
     7621 tcatgaatcc gattattttt attttattat gctttgccgc tttaggctta tttgacaaga
     7681 tgtttaagaa tcgacttgga ttagccactt cttttgatcg aggcattatc acaatgggcg
     7741 attttatgat gagcgtagga ggcttttatt gtattgcgat tgcgttttta aatggacatg
     7801 ccactctttt caaaaataaa gaaatgatta tttcaagttt gttggcgcca gatcttggag
     7861 gctattccat tgtggagtcg atgacacatg cagataatat cttgattttc tgtggtgtat
     7921 tattaacgtc cactttagga tgcttaatta gttttcaatt gccaatcttt ttaaatgagt
     7981 tggatacaga tgatttgagt cactatttaa aaggagttgt gtatggtatt ttaggattat
     8041 tacctattct tattggttgt ggctttttat tgcatattga tcattttttg attgtgtttt
     8101 tacccgtaat ttttatttgc gctattttga ctggtttatt ttttatttct tttcaaacgt
     8161 tgattgtcgt attgacatta ttttctaaac ttgtacaatt cgtaggatat atcttctttt
     8221 tcctagtttg tttgacattc tttttcaata tgaacttcac aaatgcgaca ttgatcaatg
     8281 aagcattacg cattgtattt caaatgagta ttattgtctg tggatctttg gtgttttgtg
     8341 aaattatatt aagaaagttt tcggatcaga ttgaaagagt gggtcaaata ctaaatattg
     8401 ataagtattc ggtcatggga attattttat cttttggaac aagtattgcg atgttaccat
     8461 tatttagtaa aatgaatagg aaaggtaaga ttttaaatgc tgctttttca ttgagtgggg
     8521 catttgtgtt tggtggacaa cttggtttta ttgcctcagt caaccctgca agtgtgactt
     8581 ggtttgtggt tgcaaagctt ttagctggaa tattaggctt agttattgcg aatgtattgg
     8641 aggaatgatt atggtgcgcc agttcggtgc agttgattta gttaggtgaa actcctaacc
     8701 tggtaactct tggagcaaag aggccagtat ctagtccttg ggcattgta
//
LOCUS       DBMDWL010000084         8726 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_2915_length_8726_cov_2.634667, whole genome shotgun sequence.
ACCESSION   DBMDWL010000084 DBMDWL010000000
VERSION     DBMDWL010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2915_length_8726_cov_2.634667"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /gene="ligA"
                     /locus_tag="ACZPDN_06845"
     CDS             complement(<1..1549)
                     /gene="ligA"
                     /locus_tag="ACZPDN_06845"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1298374.1"
                     /translation="MMDEKARILELRKELEKYSIEYYVYDNPSITDQEYDRLMQELMA
                     LEEKYPEMYDATSPSQRIIGSVLDGFEKVTHDTQMLSLSNVFNKEEIEEFVQRISESV
                     SNPEFVVECKYDGLAMALLYDDGNFTRAVTRGDGIVGEDVSNNVKTILSIPMHIPEMR
                     HVEVRGEVYMPKASFEQLNKRQEEKGLAPFANPRNAAAGSIRQLDSSVCASRKLDAYW
                     YYFQNASDFAVQTQEEALQAMSKMHFRTNPLRKVCTTVEQIWQFIQEIQEKREDLPYE
                     IDGMVIKLNNLADQRRLGSTAKVPRYATAYKFPAQEVLTRLKDIVITVGRTGKITPNA
                     VLEPVRVAGTTVSAAQLHNEDMIVNKDLRIGDIVVVRKAGEIIPEVVTSVPERRDGTQ
                     VPYKFPTTCPICGSHLVRLPDEAAHYCINQDCPARVVESMIHFASRDAMNIDTLGDKK
                     IEQLHEWGYLNSIEDIYFLDRYYDELIEKPGYQKKSVDKLLESIEKSKKQPLGVILYG
                     LGIRQVGK"
     gene            complement(1556..3733)
                     /locus_tag="ACZPDN_06850"
     CDS             complement(1556..3733)
                     /locus_tag="ACZPDN_06850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1298375.1"
                     /translation="MSNSILDTLNENQRTAATTINEHVRIIAGAGSGKTRVLMARIVY
                     LVQDCGVLPNRILAITFTNKAANEMKTRLTAQLGSMASVVRISTIHSLCVRMLREDAD
                     LIGYPKTFAIMDPEDQKAILKPIYKTLEVDKTTISYNRALGAISAYKTNYIDPQMAAN
                     MAMDDVSITLAKIYAGYEARRNEMKAMDFDDLLLEGHRLLNSVSSVREKWQNRLDYIH
                     VDEFQDVDPIQYGIIKLLTGKNTHLCVVGDPDQTIYTWRGASVDIILKFNKDFEPCKT
                     VILNENYRSTQPILDASNAVIKYNKERIDKDLYTKLPGDEKITMFEGKEDSEEPIFVA
                     RQINEKHKKGLEYKDMAILYRSNYSSRAFERIFKSVGIPYVIYGGIRFYERQEIKDAL
                     CYLRLCTNRNEEDPDQMALDLAVLRVINVPRRGIGARTIENLQKQAAQRHMNLYDVMK
                     DPIGLSTAVTKKCEMFVDLIEDLRENREHYALEDFLDYVLDTTGYISMLKEDRESGQD
                     RIDNLKELKEDIAQSMIEDPEMTLESYLQDIALFTDKTQETSENTVSLMTVHAAKGLE
                     FDTVFLVNFNDGVFPSSRACDEGGMKALEEERRLLYVAMTRAKKTLYITWNTGFSYMQ
                     DAFKTPSRFRAEIPMEYIVQEEKEKAPKQPKAVVSQSLTGRPSKLGANKKKVRLRKGD
                     AVEHTIYGQGVVLEIRGDVATIAFGHTIGVKKLNAAHPSIKKA"
     gene            complement(3807..3968)
                     /locus_tag="ACZPDN_06855"
     CDS             complement(3807..3968)
                     /locus_tag="ACZPDN_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298376.1"
                     /translation="MMNNKLLEKTAKANGYSSVDQLLMDYGFIPTVLTPKHKKVEIVK
                     AIAKGITNK"
     gene            complement(3958..4809)
                     /locus_tag="ACZPDN_06860"
     CDS             complement(3958..4809)
                     /locus_tag="ACZPDN_06860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR1298377.1"
                     /translation="MKLDQEQIMKFIYSAPANIFFKDTECKYCFSSEICDLVMTGEEG
                     TIIGKTDLEIQKDKACGRMYYEDDKKILATGIGSEYISEFSLDHGESMYFEIKKSAVK
                     DDDNSIIGIVGIVSDVTERVRMEKQLEESSITDSLTGVYNRNYLNYRIDEKINELEYP
                     FSVIMSDCNDLKLVNDQYGHEYGDIMLRIMASTLKEVLPKDFPIIRMGGDEFLILCNG
                     TSKAEVEEMMDTIRQSLKDKSKEKIPLSLAMGSHTVESGDFKIEDVCREADKRMYLDK
                     NQGKNDE"
     gene            complement(4903..6084)
                     /locus_tag="ACZPDN_06865"
     CDS             complement(4903..6084)
                     /locus_tag="ACZPDN_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298378.1"
                     /translation="MFVQMICKDRNEKEMNELYEVLGLIARREEVQIEDRYDHVDILV
                     CPQGKIVVTEEDGDMVLRANTRHAGPGFHAFVVDIFKDIQEEIPGEYELMDDMEFDKD
                     EDFDRLSSMYEDEMDYIRGVLLENEVMRQQNYMYDETYFLPLQKEDRILTSQGDLDLK
                     EFKHMNTRDLMDSFYVWNDWERDAKFYKNCALTLLAKEGVGKYTLMNETTIKHANDIC
                     EYIEAAYEKDTNVDLPLDAYADLCEKLGRENKLQNAKNMEQEAIQYRIKEVYHLFEDA
                     RVVASGAAERSYDPVNQALCLMSPYTDEAQWDWLIQASKQPGIVTNLDHIMEQDPIQY
                     DKKTIWMDSWQEDGIYVLEAVLRYKEKFLYFHDVCAKEKDLAFLEQCIKESGFTKTQQ
                     D"
     gene            6172..7044
                     /locus_tag="ACZPDN_06870"
     CDS             6172..7044
                     /locus_tag="ACZPDN_06870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1298379.1"
                     /translation="MNQIAKEWQDYEVIDTGNKEKLERWNQTILRRPDPVAIWPIEDE
                     RAWNKADAVYHRSKSGGGHWEMKKPIKEFWTIGYKGLRFKISPTGFKHTGLFPEQASN
                     WDFMANKIKESKRDDLRILNLFAYTGGATMACSKAGAKEVVHVDASKGMIQWAKENMQ
                     LSNLQDHTIRFIVDDCIKFVKREQRRGRKYDGIVMDPPSYGRGPNGELWKLEEELFPL
                     IQECMKILSDDALFFIVNCYTTGFSCSTLNEALSRSIQKEKGGHIECGENLLPVTTND
                     SVLPCGIFGRWTND"
     gene            complement(7091..7816)
                     /locus_tag="ACZPDN_06875"
     CDS             complement(7091..7816)
                     /locus_tag="ACZPDN_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298380.1"
                     /translation="MKATLLIKNIENLYTCDKDFTILHHAFIACHHDKIIEINTGSYK
                     EWLDPATRVIDAQGECVVPAFIDCQFKSFTHVRLGDQLRQDINALYAMRQNGILTLIC
                     DNPNSQRMKLEQDVFYKKNQPKLPVLHRLNELNDKIPETFLMSCGFGLPNSYVYSMAP
                     MSYVLFQTHRVCSRTLLESMTSLPAKEFNLLDRGSIEIGKTADLLVLQVTTIEHYFQT
                     LGRPLIHRMIKNGIQFYPEWMVC"
     gene            7876..8535
                     /locus_tag="ACZPDN_06880"
     CDS             7876..8535
                     /locus_tag="ACZPDN_06880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1298381.1"
                     /translation="MSLFNRRKKPIIVTIHGFGRNLSHEFDSLARYLKAKKYEVIQFD
                     MYDLNNPNDANYKDWVQRCEAKLSLAIKENPNVILIGFSMGGVIASYLASIYKVQSLI
                     LCAPAFEYLDIKKVTGLFKKSDKEKKGPSSKQYQAFTKIVSQYKESISQIECPILMLH
                     GTSDEVIAPSSSTHAYKKIPAQKKRLIYIEDAKHRMLYDGRMEQTLFTIIEQMLENKI
                     F"
BASE COUNT         2721 a         1726 c         1291 g         2988 t
ORIGIN      
        1 ttttaccaac ttggcgaatt cctaaaccat ataaaataac acctaatggt tgtttcttac
       61 ttttttcaat cgattccaaa agcttatcca cagacttttt ttgatagcct ggcttttcga
      121 tcaattcatc ataataacga tctagaaaat agatatcttc aatcgaattc aaatagcccc
      181 attcatggag ctgttcaatt tttttatcgc ctaaagtatc aatattcatc gcatctcttg
      241 aagcaaaatg aatcatactt tcaacaacac gagctggaca atcttggttg atacaatagt
      301 gtgctgcttc atcaggtaat cgcactaaat gggaaccaca aattggacaa gtcgttggaa
      361 acttgtatgg cacttgtgtt ccatctcttc tttcaggaac gcttgttacc acttccggaa
      421 taatttctcc agcctttcga acaacaacaa tatcaccaat acgaagatct ttattgacaa
      481 tcatatcttc attatgaagt tgagcagcac tcactgtagt tccagcaaca cgtactggtt
      541 ctaatacggc attcggtgta attttccctg tacgccctac tgtaatcaca atgtctttta
      601 atcgtgtcaa tacttcttgt gctggaaatt tgtaggctgt cgcataacgt ggaaccttcg
      661 cagtacttcc aagccttctt tgatccgcta aattatttaa tttaatgacc ataccatcga
      721 tttcatatgg tagatcctca cgtttttctt gaatttcttg aataaattgc caaatctgct
      781 caaccgttgt acaaactttt cgcaatggat tggttctaaa atgcatctta gacatagctt
      841 gcaatgcttc ttcttgtgtt tgtacagcaa aatcggaagc attctggaaa taataccaat
      901 aggcatctaa tttacggcta gcacatacgc ttgaatccaa ttgtcgaatg cttcctgcag
      961 ctgcatttct tggattcgca aaaggggcca agcctttttc ttcttgtctt ttatttaatt
     1021 gttcaaagct agccttaggc atatagactt caccacgaac ttcaacatga cgcatttcag
     1081 gaatatgcat aggtatggaa agaatggttt tcacattatt tgaaacatct tctccaacaa
     1141 ttccatcccc tcttgtcacg gctctagtaa agtttccatc atcataaagt aaagccatcg
     1201 caagtccgtc gtatttgcat tctacgacaa attctggatt tgaaacactt tctgagattc
     1261 tttgaacaaa ttcttcaatt tcttctttat taaagacatt tgacaaagaa agcatctgcg
     1321 tatcatgtgt cactttttca aagccatcaa gtacagagcc aatgattctt tgactaggac
     1381 ttgtcgcatc atacatttct ggatattttt cttccaatgc cattaattcc tgcattaaac
     1441 gatcgtattc ttgatcggtt atgcttggat tatcatatac ataatattcg atagaatact
     1501 tttctaattc tttacgtaat tctaaaattc ttgccttttc atccatcata ctcacctagg
     1561 ctttctttat agaaggatgt gctgcattta attttttcac accaattgta tgaccaaagg
     1621 caatcgttgc gacatctcca cgaatttcaa gtacaacacc ctgaccataa attgtatgtt
     1681 caacggcatc ccctttacgt aaacgcactt tctttttatt cgcaccaagc ttagatggtc
     1741 ttcctgttaa ggattgagaa acgacagcct ttggttgttt tggtgccttt tctttttctt
     1801 cttggacaat atattccatt gggatttcag ctctaaaacg acttggtgtt ttaaacgcat
     1861 cctgcatata actaaagcct gtattccatg taatgtatag tgttttttta gcacgtgtca
     1921 tcgcaacata tagaagtctt ctttcttctt ctaaagcttt catgccacct tcatcacaag
     1981 ctcttgaact tgggaacaca ccatcattaa agttaactaa gaatactgta tcaaactcta
     2041 atccttttgc ggcatgcact gtcattaagc ttactgtatt ttcacttgtt tcttgtgttt
     2101 tatctgtaaa taaggcaatg tcttgtaaat aagattctaa agtcatttcc ggatcttcga
     2161 tcatactttg ggcaatgtct tcttttaatt cttttaagtt atcaatacgg tcttgaccac
     2221 tttcgcgatc ttctttcaac atcgaaatat agcctgttgt atctaataca taatccaaaa
     2281 aatcttctaa ggcataatgc tccctatttt cacgtagatc ttcaattaaa tctacaaaca
     2341 tttcacattt tttcgtaact gctgtactta atccaattgg atctttcatg acatcatata
     2401 gattcatgtg tctttgagcg gcttgttttt gaagattttc aattgtccgc gcaccaatac
     2461 cacgtctagg cacattgatc acacgtagta cggctaaatc taatgccatt tggtctggat
     2521 cttcttcgtt acggtttgta cataatctta aataacatag cgcatctttg atttcttgtc
     2581 gttcatagaa acgaattcca ccataaatca catatggaat acctacggat ttaaaaattc
     2641 tttcaaatgc acgtgaagaa tagtttgaac gataaagaat cgccatatct ttatattcca
     2701 aacctttttt atgtttttca ttgatttgac gagccacaaa gattggttct tcgctatctt
     2761 cttttccttc aaacatcgtg atcttttcat caccaggaag ttttgtgtat aaatctttat
     2821 caatacgttc cttattgtat ttaataaccg cattacttgc atctagaata ggttgtgttg
     2881 aacgatagtt ttcattcaag atgaccgttt tacaaggttc aaaatcttta ttaaatttca
     2941 agataatatc aacacttgct ccacgccaag tatagattgt ctggtctgga tctcctacaa
     3001 cacataagtg tgtattttta cctgttaata gcttgatgat tccatactga atagggtcca
     3061 catcctggaa ttcatctaca tgaatgtaat ctaaacgatt ttgccatttt tctcgaacag
     3121 aagaaacact atttaataag cgatgtcctt ctaataataa atcatcaaaa tccatagctt
     3181 tcatttcatt acgacgtgct tcgtaacctg catagatttt agctaaagta atacttacat
     3241 catccatagc catatttgcg gccatttgtg gatcaatata gtttgtctta taagcactaa
     3301 tagcaccaag tgcacgatta taagaaatcg ttgttttatc gacttctaat gttttataaa
     3361 ttggttttaa gatggccttt tgatcttccg gatccataat tgcgaatgtt tttgggtatc
     3421 caatcaagtc agcatcttct ctaagcattc ttacacataa agagtgaatt gttgaaatac
     3481 gcaccacact tgccatactg cctagttgtg cagttaatcg agttttcatt tcatttgctg
     3541 ctttatttgt aaatgtaatg gccaagatac gatttggtaa aacgccacaa tcttggacca
     3601 aataaacaat acgtgccatt aagacacgtg tttttccgct acctgcccct gcaataatac
     3661 gaacgtgttc gttaattgtc gttgcagccg ttctttgatt ttcatttaat gtatctaata
     3721 ttgagttact cataattttt aatcaccgta tcaattttac cataaaacac ataaaaagtg
     3781 ctcagaaata tgagcactcc attctactat ttattggtta tacccttcgc aatcgcttta
     3841 acaatttcta ccttcttatg ttttggtgtt aatacagtag gaatgaatcc ataatccatc
     3901 aacaattgat caacagatga atagccattt gctttagctg tcttttctaa aagtttatta
     3961 ttcatcattc tttccttgat tcttatccaa atacattcgt ttatctgctt ctctacatac
     4021 atcttcgatt ttaaaatctc cagattcgac tgtatgactt cccatcgcaa ggcttaacgg
     4081 aatcttttct ttactcttat cttttaaact ctgtcgaatt gtatccatca tttcttctac
     4141 ttctgctttt gaagtgccgt tacaaagaat caagaattca tctccaccca tacgtataat
     4201 tggaaaatct ttaggcaaaa cttcttttaa tgtactagcc ataattcgta acataatatc
     4261 cccatattca tgtccatatt gatcatttac taactttaaa tcgttgcaat ccgacataat
     4321 gactgaaaat ggatattcta attcattaat tttttcatca atccgataat ttaaataatt
     4381 tcgattatat acgcctgtta atgaatctgt gattgaagat tcttcaagtt gtttttccat
     4441 acgaacacgc tctgtcacat ccgatacgat gcccacaatt cctattattg aattatcgtc
     4501 atctttgaca gcacttttct tgatctcgaa atacatactt tctccatgat ccaacgaaaa
     4561 ttcactaata tattcactgc ctataccagt cgcaagtatc tttttatcat cctcataata
     4621 cattcttcca cacgctttgt ctttctgaat ttctaaatcg gtctttccaa taattgtgcc
     4681 ctcttctcct gtcatgacaa gatcacatat ttcagaagaa aaacaatatt tacattctgt
     4741 atccttgaaa aatatattgg ctggcgcact atatataaac ttcatgattt gttcttgatc
     4801 taatttcatt tgattatctc catcacgtta cgtatcaaat ccattatata ctttatttaa
     4861 agcactttcc ataagaaaaa aagacaatgt caaatgacat gtctaatcct gttgcgtttt
     4921 tgtaaaacca gattccttaa tgcattgttc taagaaagct aaatcctttt ctttagcaca
     4981 tacatcgtgg aaatataaga atttttcttt gtagcgaagt acagcttcta atacatagat
     5041 tccatcttct tgccatgaat ccatccaaat cgttttctta tcatattgga ttggatcttg
     5101 ttccataata tgatctaagt ttgtgacaat acccggttgt ttactcgcct gaatcaacca
     5161 atcccattgt gcttcatctg tatatggaga cattaaacat aaggcttgat ttactggatc
     5221 atagcttctt tcagctgcac cactggcaac aactcttgca tcttcaaata agtgatacac
     5281 ttctttaata cgatattgaa tcgcttcctg ctccatattt ttggcattct gtaatttatt
     5341 ttctctaccc aacttttcac ataagtctgc ataggcatct aatggaagat ctacattcgt
     5401 atccttttca taagccgcct cgatatattc acaaatatca ttggcatgct taatcgttgt
     5461 ttcattcatt aatgtatact ttccaacacc ttctttggca agtaaagtca atgcacaatt
     5521 tttatagaat tttgcatctc tttcccaatc attccaaaca tagaaagaat ccatcaagtc
     5581 acgagtattc atatgtttaa attcttttaa atccaaatcc ccctgacttg taagaatgcg
     5641 atcttctttt tgaagaggta agaaatacgt ttcatcatac atataatttt gttggcgcat
     5701 cacttcattt tccaatagaa caccacgaat ataatccatt tcatcctcat acatagaact
     5761 caatcgatca aaatcttcat ccttatcaaa ttccatatca tccattagtt catattcgcc
     5821 aggaatttct tcctgaatat ctttaaatat atctactaca aacgcatgaa atcctggacc
     5881 tgcatgacgt gtattagcac gtaaaaccat atcaccatct tcttctgtca caacaatctt
     5941 tccttgtgga caaactaaga tatcaacatg atcataacga tcttcaattt gcacttcttc
     6001 tcgtcttgcg ataaggccta aaacttcata caattcattc atttcttttt catttcgatc
     6061 tttacaaatc atttgaacaa acatgttaaa acctccttct agagtcacac tctattatat
     6121 catagttggc aaatgtatgt tataataaag cggttaaagg agaactaaag tatgaatcaa
     6181 attgctaaag aatggcaaga ctatgaagtt attgatactg gaaataaaga aaaactagaa
     6241 agatggaatc aaacgattct tagaagacca gatcctgtgg caatctggcc aatcgaagat
     6301 gagcgtgcat ggaataaagc cgatgctgta tatcaccgtt ctaaatcggg gggtggtcat
     6361 tgggaaatga aaaagcctat taaagaattc tggacaattg ggtataaagg attacgcttt
     6421 aaaatcagtc caactggatt taaacacaca ggtctattcc cagaacaagc tagcaactgg
     6481 gattttatgg ccaataagat caaagaatca aaacgtgatg atttaagaat cttaaatcta
     6541 tttgcctata caggtggtgc taccatggcc tgctcaaaag cgggagcgaa agaagttgtg
     6601 catgttgatg cttcaaaagg tatgattcaa tgggcaaaag aaaacatgca actttcgaat
     6661 ttacaagatc atacgattcg ttttattgtg gatgattgta ttaaatttgt aaaaagagaa
     6721 caaagaaggg gaagaaaata cgatggtatc gtcatggatc cacctagtta tggtagaggt
     6781 ccaaatggtg aactttggaa attggaagaa gaattattcc cattgattca agaatgtatg
     6841 aagattttaa gtgatgatgc cctattcttt attgtgaact gttatacaac tggatttagt
     6901 tgttcaacac tcaatgaagc tttatctaga tcgattcaaa aagaaaaagg tggacacatt
     6961 gaatgtggtg aaaacctgct tcctgttacg acaaatgata gtgtattacc atgcggaatc
     7021 tttggaagat ggacaaatga ctagtccatc tttttttgtt tagaaaaaga aaaaaggaag
     7081 aaaactcttc ctaacaaacc atccactcgg gatagaattg aatcccattt ttaatcatac
     7141 gatgaatcaa aggtcttcct aaagtctgaa aataatgttc aatcgttgtg acttgtaaaa
     7201 ccaaaagatc tgccgttttt ccaatttcaa tcgatccacg atctagtaaa ttaaattctt
     7261 tagctggcaa agacgtcata ctttctaata atgttctcga acacactcga tgtgtttgaa
     7321 acaatacata actcataggt gccatactat atacgtagga gtttggaagt ccaaatccac
     7381 aactcattaa aaaagtttct ggaattttat catttaattc atttaaacga tgtaaaaccg
     7441 gaagcttagg ctgattcttt ttataaaaaa catcctgttc caacttcatt ctttgcgaat
     7501 ttggattgtc acaaatcaaa gttaagattc cattttgacg catggcatat aacgcattga
     7561 tatcttgtct tagttgatca cctaaacgta cgtgtgtaaa acttttaaat tgacaatcaa
     7621 taaaagccgg cacaacgcat tcaccttgcg catcaatcac tcgtgttgca ggatcaagcc
     7681 attctttata agaacccgta ttgatttcaa taatcttatc atggtgacat gcaataaaag
     7741 catgatgtaa gattgtaaaa tccttatcac atgtatataa attttcaata tttttaatca
     7801 gtaaagttgc cttcatgata catgtgacct tcctttcaat agatgttata ataattatac
     7861 aacgaggtga tgcgaatgtc tttatttaat cgaagaaaga aacctataat agttacaatc
     7921 catggatttg gaagaaatct aagtcatgaa tttgattcat tagcgcgcta tttaaaggct
     7981 aaaaaatatg aagtgattca atttgatatg tatgatttaa acaatccaaa tgatgccaac
     8041 tacaaagatt gggtacaaag atgtgaagcc aaattaagtc ttgccattaa agaaaatccc
     8101 aatgtcattc taattggatt ctccatggga ggtgtaatcg ccagttattt ggctagcatt
     8161 tataaagtgc aatccttaat cctttgtgca ccggcctttg aatatcttga catcaaaaaa
     8221 gtaacgggtt tatttaaaaa atccgacaaa gaaaagaaag gcccaagctc aaagcaatac
     8281 caagccttca caaaaatcgt ttcccaatat aaagaatcta tttctcaaat cgaatgtcca
     8341 attcttatgt tgcatggaac aagtgatgaa gtgattgctc catcaagttc tactcatgcc
     8401 tataaaaaga ttccagcaca aaagaaacgc ctgatttata ttgaggatgc caaacataga
     8461 atgttgtatg atggaagaat ggaacagact ttatttacaa tcatcgaaca aatgcttgaa
     8521 aacaaaatat tctaaaaagc tgtcgaataa gacagctttt tcatttattg ccaactataa
     8581 aatatgcttt atctaaagat attctagcat attttcattt ccatttgcac ctttaaggac
     8641 ttttttcact tctccatttg cacctttaag ggcttttttc ttcttatgat ttgcacctac
     8701 aaagaaaaaa agcctgtatt tctaca
//
LOCUS       DBMDWL010000085         8494 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3024_length_8494_cov_3.705588, whole genome shotgun sequence.
ACCESSION   DBMDWL010000085 DBMDWL010000000
VERSION     DBMDWL010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8494)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_8494_cov_3.705588"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="ACZPDN_06885"
     CDS             complement(<1..634)
                     /locus_tag="ACZPDN_06885"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HIR1298382.1"
                     /translation="MNLEKWIEEKFNTTKYTLEKTDKGISNYSYVLTINDTKYMIRVP
                     KKNHESLGLQHEHEKEILPLVSDLDVPTVLFDEKEGIKVTHYIEDVKTFDECKDKDKF
                     ARCANLMKSLHNKKAPLFIFNPFGKIEFYKSQIKECILSFPNEDNFLEALKKEYKPNT
                     LCHNDFVQGNILYSDTKDYLIDYEYAAKNDYRFDIASFFSENNIHYIDQRD"
     gene            814..1449
                     /locus_tag="ACZPDN_06890"
     CDS             814..1449
                     /locus_tag="ACZPDN_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1298383.1"
                     /translation="MKYKVKWIEDHMSITRNMIRRYEKKGVILRNEKGKDREFDEDDL
                     IQLWNIRVMVSLGFSLDDVKEVLYGSNLREVSEKRLRALNEEYRDLQGKINFLNVVKI
                     TGEMPSCFKVSANNFEEIYNLGLIQYVSPFERAKDIWALMDMLQLLEKLCDTSDKKIM
                     DELYEWFGIGNDREMFVQVFETYLLCDERFEEIFGKEKCCELSALIATYGK"
     gene            1460..2188
                     /locus_tag="ACZPDN_06895"
     CDS             1460..2188
                     /locus_tag="ACZPDN_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298384.1"
                     /translation="MKKLMCLSLCFVLCGCGAMPTAQNVEVKKVNVNVIEVSASSLDE
                     IEEMATKDVEDTKEKLESKRDALSEEILDFDAYTKNVNKVKAYYEDALKQTELLSIRL
                     REYAYKYAELIMKEDTSYKEKYKDLSGIYEYIYDDAAKTMYDIYDKTLKDMYDIYYDG
                     IIKAAYDVVDYEQWYDARSDAYDDWYDARSDAYDIWYDTRSDIYDFQYDLRSEVYDHD
                     DKRAQKKMDKFKKSILRMKEDVND"
     gene            2332..2751
                     /gene="rplK"
                     /locus_tag="ACZPDN_06900"
     CDS             2332..2751
                     /gene="rplK"
                     /locus_tag="ACZPDN_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1298385.1"
                     /translation="MSKKKVTKVCKLQFQAGGAKPGPALASAGINMPQFCTAFNDATK
                     DRKGDIVPVVITAYEDKSFDFVLKTTPAANLLKKAANVQKASGDQKVVAGTITVDQLK
                     EIAEYKMPDLNANDVEAAMRIVAGTARNMGIKVEGFE"
     gene            2766..3452
                     /gene="rplA"
                     /locus_tag="ACZPDN_06905"
     CDS             2766..3452
                     /gene="rplA"
                     /locus_tag="ACZPDN_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR1298386.1"
                     /translation="MAKVSKRYAEAAKLVDRSKTYSIEEAVKLAKETSKTKFDASVEV
                     SFRLNVDPRHADQQIRGAMVLPNGTGKTQRVCVVAQGEKEKEAQAAGADFVGGADIIE
                     KISKGWFDFDVLVATPDMMGQLGRLGRVLGPKGLMPNPKTGTVTMDVAKAIDEIKKGK
                     VTYRVDKEGNINLAIGKTSFTEEALVENFNTIFNTIAKARPATIKGAYMKNLVVSTTM
                     GPGIHVEIVK"
     misc_feature    3483..3602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3635..4174
                     /gene="rplJ"
                     /locus_tag="ACZPDN_06910"
     CDS             3635..4174
                     /gene="rplJ"
                     /locus_tag="ACZPDN_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR1298387.1"
                     /translation="MNKDILAQKEADVIALSEKMEAASSIVLVEYRGLSVKEVTELRR
                     ALREEDVEFKVYRNAIARRAAEKLGHEDFAKTLVGPNALAFGKDPVAPARVLAKFAKN
                     HEALVLKTGIVDGAMVDEATIQKLSALPNKEGMLAKFASCLNAPVIKFAMTVKALAEA
                     KENGSVKTEEAPATEEAAA"
     gene            4197..4565
                     /gene="rplL"
                     /locus_tag="ACZPDN_06915"
     CDS             4197..4565
                     /gene="rplL"
                     /locus_tag="ACZPDN_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR1298388.1"
                     /translation="MAKLTQEEILSYLEEATILELNDLVSAIEEKFGVVAAAAVAAPA
                     AAAEEAAGPSEVTVTLTNVGGTKVAVIKAVREITGLGLVDAKKLVDGVPAAIKENVSP
                     EEAESIKEKLVAAGATVEIK"
     gene            5152..6450
                     /gene="ltrA"
                     /locus_tag="ACZPDN_06920"
     CDS             5152..6450
                     /gene="ltrA"
                     /locus_tag="ACZPDN_06920"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR1298389.1"
                     /translation="MELIEKILSEENLQKAIRKVKKNKGAPGVDKMTVQEVEEWFNQY
                     KEDLISKILNKQYKPMPVKRVYIPKPNGKQRPLGIPTVVDRVIQQAMLQVLNEIYEPV
                     FSEHSYGFRPNRSAHMAMEEVLSYLNDGYEWIIDLDIEKFFDTVNHDKLISILRERVN
                     DSKTLHLIRAYLQAGILDKGLVRSSTIGTPQGGPISVILSNIYLDKFDKELESRNLRF
                     ARYADDCIIFVKSEMSANRVMKSVTSWLERKLFLKVSATKTKVVRPTKGQFLGFTFYK
                     NSDEWKCCHTKDRKKRLYSNIQKWMERKHAVSRPLSVTFEKLNQMVRGWIQYFKISSI
                     KGFLEEFGQWLRHKVRCIIIKQWKRPMTIYNNLMKLNKACRSNLTHEDIFKCANSRLG
                     WYRRSCGNIVNFLLSSTVLGIKKGDRPGLVNPLDYYLEIL"
     gene            complement(6545..8419)
                     /locus_tag="ACZPDN_06925"
     CDS             complement(6545..8419)
                     /locus_tag="ACZPDN_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIR1298390.1"
                     /translation="MNERQKNILKKMLETNDCFTSNHISDTFNISLRTAQNDIKAIVN
                     YCEEEHIEYTYNKKDGYRVVSQSKIVQNTENTDSNSIRLKYILRLLLFSKEPIKSEKI
                     ASSLFISNTTLLKEMIFVKQVLAKYNLTIKSKPYHGSIILGYEHDKRNCIINENLITF
                     HDEVVIHHHKPYIDMDCLHTVSLIVSNVLITNKFNITDSDFQNFLMIAYLSTIRSIDS
                     CYLENNFLNDFNFNETVHIAKKIIEKLSNQYHFKICDSEIRFLASVLHGKNSLITSEI
                     IPSYIENDVSTILDQINSTLSIDFSYSIELRLSLSLHLIPLYERISADNQLTEIPITN
                     IHNIFSYSFELALIASQYINKIKNTELSEAEIAYLAIHFNVILEKDGNAKEKKNVLFI
                     CSSRRSDSLLIKSGIYNHFSNRISQIDVKNLYEIHSINPSEYDVIFSTTLNDKRVPKN
                     AIKLNHFLTNTDYNTIEFALNHGSIFKMIEPLFSKSRFITNLKAENKEKVISELTKLS
                     ANIIESDVLYEAILQREQNGYTSYGNRIALPHPNYLLAKKSFCSVAILDKPILWSENN
                     KAQIIILCCASKNSGKDLQMLFNFISLLFNNTEKVNHLIKHPDYDDFMHCLKSLQNNE
                     "
BASE COUNT         2900 a         1129 c         1753 g         2712 t
ORIGIN      
        1 gatctctttg gtcaatgtag tgaatattgt tctctgaaaa gaaactggcg atatcaaagc
       61 gataatcgtt tttagctgcg tattcataat caatcaaata gtcttttgta tcgctgtaaa
      121 gaatatttcc ttgtacaaag tcgttatgac atagtgtatt tggtttatat tcttttttta
      181 atgcttctaa aaagttatct tcatttggaa aagataggat acattctttg atttgtgatt
      241 tataaaattc aatttttcca aaaggattaa aaataaataa aggagctttt ttgttatgaa
      301 gagatttcat taaatttgca caccttgcga atttgtcttt gtccttacat tcatcaaatg
      361 tttttacatc ttcaatataa tgagtgactt taatgccttc tttttcgtcg aataaaacag
      421 tgggcacatc taaatcggaa acaagtggca aaatttcctt ttcgtgttca tgttgtaagc
      481 caagtgattc atgattcttt ttaggtacac gtatcatgta tttggtgtcg ttgattgtta
      541 atacatagct gtagttacta atgcctttat ctgttttttc aagtgtatat tttgttgtgt
      601 taaatttttc ttcaatccat ttttctaagt tcatgtggat atattaacat tattattctt
      661 taaactcaat gcggtcatta tgccgctttt tttgttcgcg aaatctgcaa gggctcttat
      721 ggcaaaaaag tgtggaagga ggatagaaga aataactttt tgcttgcgtt gttcttggaa
      781 caaggctgta gattgtataa ggagtcatgc actatgaaat ataaggtgaa atggattgaa
      841 gatcatatgt caatcacaag gaatatgatt cgtagatatg aaaagaaagg agtgatttta
      901 agaaatgaaa agggaaaaga tcgagagttt gatgaagatg acttgattca attatggaat
      961 ataagggtaa tggtaagttt aggttttagt ttggatgatg tcaaagaagt tttgtatgga
     1021 tcaaatttaa gagaggtgag tgagaaaaga ttaagggctt tgaatgagga gtatagagat
     1081 ttacaaggga aaatcaattt tttaaatgta gttaaaatta caggggaaat gccttcgtgt
     1141 tttaaagtga gtgcaaataa ttttgaagaa atctataatt tagggcttat acaatatgta
     1201 agtccgtttg aaagagcaaa agatatttgg gcgttgatgg atatgttgca gcttttggaa
     1261 aagttatgtg atacaagtga taaaaaaata atggatgaat tgtatgaatg gtttggaata
     1321 ggcaatgata gagagatgtt tgtacaggtg tttgaaacgt atttattgtg tgatgaaagg
     1381 tttgaagaaa tatttggaaa ggaaaaatgt tgtgagttat cggctttaat agctacttat
     1441 ggaaagtgag tgaataataa tgaaaaagtt gatgtgttta agtttgtgct tcgttctttg
     1501 cggatgtggg gctatgccta ctgcacagaa tgttgaagta aagaaagtaa atgtgaatgt
     1561 aattgaggtg tctgcttctt cgcttgatga aatagaggag atggcaacaa aggatgttga
     1621 ggatacaaag gaaaagttag agagtaaaag ggatgcattg agtgaggaga ttttggattt
     1681 tgatgcatat acgaaaaatg tgaataaggt aaaagcgtat tatgaggatg ctttaaaaca
     1741 gacagagttg ttgtccattc gattgaggga atatgcttat aagtatgctg agttgattat
     1801 gaaagaagat acatcgtata aggagaaata caaagacttg agcggaatct atgagtatat
     1861 ttatgatgat gcggctaaga ctatgtatga catatacgat aagacactaa aagatatgta
     1921 tgatatatat tatgatggaa taattaaggc tgcgtatgat gttgttgatt acgagcagtg
     1981 gtatgacgca cgttcagacg cgtacgatga ttggtatgat gcaagaagtg atgcttatga
     2041 tatatggtat gatactcgtt ctgatattta tgattttcaa tatgatttga gaagtgaagt
     2101 atatgatcat gatgacaaaa gagcacagaa gaagatggat aagtttaaaa agtcaattct
     2161 tcgtatgaag gaggacgtga atgattagag agaatcaatg attttctcta atttttttaa
     2221 aatgtgcttg catcactgga tgtagtggtg tatagtaaat aggcatgcgt gggagaatgc
     2281 gtaactgttc gtattaccac agcataggac aaaatatagg aggaacgtct tatgagcaag
     2341 aaaaaagtta caaaagtttg taagttgcag ttccaagctg gtggagctaa accaggtcca
     2401 gcattagcaa gtgctggtat caacatgcct cagttctgta cagctttcaa tgatgcaaca
     2461 aaagatcgta aaggtgatat tgttccagtt gtcattactg cttatgaaga taagagtttt
     2521 gatttcgtat tgaaaacaac acctgctgct aacttattga aaaaagcagc aaacgtacaa
     2581 aaagcaagtg gagatcaaaa agtagttgct ggtacaatta cagttgatca attaaaagaa
     2641 attgctgagt acaaaatgcc agacttgaat gccaacgatg tagaagctgc aatgcgtatt
     2701 gttgctggta ctgcacgtaa catgggtatc aaggttgaag gatttgaata ggaggaacat
     2761 caataatggc aaaagtaagt aagagatatg ctgaagcagc taaattagtt gatcgttcaa
     2821 aaacttattc aattgaagaa gctgttaaat tagctaaaga aactagtaaa acaaaattcg
     2881 acgcaagtgt tgaagtaagt ttccgtttaa acgtagaccc tcgtcacgct gatcaacaaa
     2941 tccgtggggc aatggtatta cctaatggaa ctggtaaaac acaacgcgtt tgtgtagttg
     3001 cacaaggcga aaaagaaaaa gaagctcagg ctgctggtgc tgatttcgtt ggtggtgcag
     3061 atattatcga aaaaatctct aaaggttggt tcgattttga tgtattagta gctactccag
     3121 atatgatggg tcaattagga cgtttaggac gtgttttagg tcctaaagga ttaatgccaa
     3181 accctaagac tggtacagta acaatggatg ttgctaaggc tattgatgaa atcaaaaaag
     3241 gtaaagttac ttaccgtgta gataaagaag gaaatatcaa cttagctatc ggtaaaacaa
     3301 gctttacaga agaagcatta gttgaaaact tcaatacaat cttcaataca attgctaagg
     3361 ctcgtcctgc aactattaaa ggtgcataca tgaagaactt agttgtttca acaacaatgg
     3421 gtcctggaat ccacgttgaa atcgttaagt aattgacaaa tctagtaaaa taaattaaac
     3481 tacagattgt tatcaatatt ccaaagacag taacggcgta tgcttaataa tgttaccgag
     3541 ggtatgatgc attgactttg cacaattagc ccgtctttga tgcgggcttt tcttttgaat
     3601 attgttacaa tagatgaaag aagaggtgga tcgaatgaac aaagacatcc ttgcacaaaa
     3661 ggaagctgac gttatcgcct tatctgaaaa aatggaagct gctagtagta tcgtattagt
     3721 agaataccgt ggattatcag taaaggaagt aacagaactt cgtcgtgctt tgcgtgaaga
     3781 agacgttgaa ttcaaagttt accgtaacgc aatcgctcgt cgtgcagctg aaaaattagg
     3841 ccacgaagat ttcgcaaaaa ctttagttgg acctaacgca ttggcatttg gtaaagatcc
     3901 agttgctcct gctcgtgtat tagcaaaatt cgctaaaaat catgaagcat tagtattaaa
     3961 aactggtatc gtggatggag ctatggtaga cgaagctact atccaaaaat tgtctgcatt
     4021 acctaacaaa gaaggtatgt tggctaaatt cgcatcttgc ttaaatgctc cagttatcaa
     4081 gtttgctatg actgttaaag cattggcaga agctaaagaa aacggatctg taaaaacaga
     4141 agaagcacca gctacagaag aagctgctgc gtaattaatt ataggaggaa aataaaatgg
     4201 ctaaattaac tcaagaagaa attctttctt atttagaaga agcaacaatt ttagaattaa
     4261 acgacttagt aagtgcaatc gaagaaaaat ttggtgtagt agctgctgct gctgttgctg
     4321 ctccagctgc tgctgctgaa gaagctgcag gacctagcga agtaactgtt acattaacaa
     4381 atgttggtgg aacaaaagtt gctgtaatca aagccgtacg tgaaattact ggtttaggat
     4441 tagttgacgc taagaaatta gtagatggtg ttccagctgc tattaaagaa aacgtatctc
     4501 cagaagaagc agaatcaatt aaagaaaaat tagttgctgc tggagctact gtagaaatta
     4561 agtaatttaa gcttaataag taacttaaag cctttgtctt cggatagagg ctttttttgt
     4621 gtgtgctggg catggcatga gttcagttgg agttaaacca atcacgggta gttaccgcca
     4681 agtgtagtga accacaagcc gttgacagtg atgtcatggg tggaggaagt ggtgtagcga
     4741 ttctttcgag cgtacgcaga gaaacttgat gaggctaaat ggtggatgag attgcgcaac
     4801 aaatcgaagt ccaaaaggta aacgtaaaac caagacagta aatcaagacg ttgtgaaaga
     4861 aatagggctt atgtcttacc ccgggaggtc tcatggacaa ggaattctag taaactttaa
     4921 aatcagtgat agtacccttg tagcgaaaaa ggttaaccat gagaaatcag atgaagtcat
     4981 agtaggtagc aatactgaag gactgaaacg tttatagagt acaaatctct attagcaatg
     5041 tttggatagt ccgaatatga gaatagccta gaactatgga acgtaaccat agatggtaaa
     5101 ggtaattggg atgtttccaa ataaatttac aaatagaagg aggagcagca aatggaattg
     5161 attgaaaaga ttttaagtga agagaatcta cagaaagcaa ttcgaaaggt caagaagaac
     5221 aaaggtgcac caggagtgga caaaatgacc gtgcaagagg ttgaggagtg gtttaatcaa
     5281 tataaggaag acctaatttc caaaattttg aataaacaat acaagcccat gcctgtcaaa
     5341 agagtctata ttcctaagcc aaatggcaaa caaagaccat tgggtatacc aactgttgta
     5401 gaccgagtaa tccaacaggc tatgttacaa gtgctaaatg aaatctatga accagtattt
     5461 agtgagcaca gttatggctt tagaccaaat cgtagtgcac acatggcgat ggaagaagtg
     5521 cttagctatc taaacgatgg atatgaatgg attattgacc tggatattga gaaattcttc
     5581 gacacagtaa atcatgataa attaatttca atcttaagag agcgtgtcaa tgatagtaaa
     5641 acattacatt tgatacgagc atatcttcag gcaggaattt tggataaagg actagtaaga
     5701 agttctacta ttggaacgcc acaaggtggt ccaattagtg taattctttc aaacatctat
     5761 ttagacaagt ttgataaaga attagaatct agaaatcttc gcttcgctag gtatgctgac
     5821 gactgtatca ttttcgttaa aagcgaaatg agtgcaaatc gtgtaatgaa gtcagtgaca
     5881 tcgtggctag aaagaaaact attcttgaag gttagtgcaa caaagactaa agtagttaga
     5941 ccaactaaag gtcaatttct tggttttaca ttttataaga atagtgacga gtggaaatgt
     6001 tgtcatacta aagataggaa gaaacgtctg tactcaaata ttcagaaatg gatggaaaga
     6061 aaacatgcag tttcaagacc tctctcagtt acatttgaaa aattgaatca aatggtcaga
     6121 ggttggatac aatactttaa aatctcaagt atcaaaggtt tcttggaaga atttggccag
     6181 tggttacgtc ataaagttag atgtatcatc attaaacaat ggaaaagacc gatgactata
     6241 tacaacaatc ttatgaagct aaataaagca tgcagaagta atcttaccca tgaagatata
     6301 tttaaatgtg ctaactctag acttggatgg tacaggagaa gttgtggaaa tattgtgaac
     6361 tttcttcttt cttctactgt tctaggcata aagaaaggag atagacctgg tctggtcaat
     6421 ccccttgatt actatcttga gattttgtga ttcgttatag tttcgatata aatgtagagc
     6481 cgtatacgag acccgtacgt acggttcaac gggaggggcg ataattttat tttatccctc
     6541 taccctattc gttattttgt aggcttttta gacaatgcat aaaatcgtca taatctggat
     6601 gcttgattaa atgatttact ttttcagtgt tgttaaatag caaggaaata aagttgaaca
     6661 acatctgtag atcttttccg gaatttttag aagcacagca taatatgata atctgggctt
     6721 tattgttttc actccataga attggcttgt ctaaaatagc tacagaacag aatgattttt
     6781 ttgctagtaa ataatttggg tgtggcaaag caattcggtt tccatatgat gtatatccat
     6841 tttgttctcg ttgtaaaata gcttcataca gaacatctga ctcaattata tttgcggata
     6901 gtttagttaa ctcggaaatc actttttctt tgttttctgc tttgaggttt gtgataaagc
     6961 gtgactttga aaataaaggt tctatcattt taaaaataga tccatgatta agtgcaaatt
     7021 caatcgtatt ataatctgta tttgtaagaa agtgatttaa tttgatggca tttttcggaa
     7081 cccgtttgtc attcagtgtt gttgaaaaaa tcacatcata ttcgcttggg ttaatactat
     7141 gaatttcata tagattcttt acatcaatct ggctgatacg atttgaaaaa tgattatata
     7201 ttccactttt gattaataga gaatcacttc ttcgtgagga acatatgaat aaaacatttt
     7261 ttttctcttt cgcgtttcca tctttttcta ggataacatt aaaatgtata gctaaataag
     7321 caatttctgc ttctgataat tctgtatttt taattttatt gatatattga gatgcaatta
     7381 atgctaattc aaacgaatag gaaaaaatat tatgtatatt cgtgattgga atttcagtta
     7441 gttgattgtc tgcacttatt cgctcatata aagggataag atgtaatgat agggataaac
     7501 gaagttcaat gctgtaggaa aaatcaatac ttaatgttga attgatttga tctaaaatcg
     7561 tcgaaacatc attttcaata taggatggta tgatttctga agtaatcagg ctgtttttac
     7621 catgcagtac agatgctaaa aaacgtattt ctgaatcaca gattttaaaa tggtattgat
     7681 ttgataactt ttctatgatc tttttagcaa tgtggacagt ttcattaaaa ttaaaatcgt
     7741 tcaaaaaatt attttccaag taacaagaat caattgaacg tattgtactt aagtatgcaa
     7801 tcattaagaa gttttgaaag tcgctgtctg taatattaaa tttgtttgta attaaaacat
     7861 tggatacaat taggcttaca gtatgtaaac aatccatatc aatatatggc ttatgatggt
     7921 gaataactac ttcatcgtga aaagtaatta agttttcatt aataatacag tttcttttat
     7981 cgtgttcata gccaaggatt attgagccat ggtatggttt gcttttaatg gtaagattgt
     8041 attttgctag aacttgttta acaaaaatca tttcttttaa taacgttgta tttgagataa
     8101 aaagactaga agcaattttt tctgacttta taggttcttt tgaaaataac agcaacctta
     8161 aaatatattt taatcgaata gaatttgaat ctgtattttc agtattttgt acgatttttg
     8221 attgagaaac cactctatat ccatcttttt tattataggt atattcaata tgctcttctt
     8281 cacaataatt aacaattgcc tttatatcat tttgggctgt tcttagcgaa atattaaatg
     8341 tatcagatat atgatttgat gtaaaacaat catttgtttc caacattttt tttaatatgt
     8401 ttttttgcct ttcattcatg aaataacctc ttagattatg ataatttaaa tgatttagaa
     8461 cttcaataat gaatattgcg tttgacgcaa tata
//
LOCUS       DBMDWL010000086         8438 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3063_length_8438_cov_2.726520, whole genome shotgun sequence.
ACCESSION   DBMDWL010000086 DBMDWL010000000
VERSION     DBMDWL010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_8438_cov_2.726520"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            159..434
                     /locus_tag="ACZPDN_06930"
     CDS             159..434
                     /locus_tag="ACZPDN_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR1298391.1"
                     /translation="MANTTNFSVRMDSDIKKQCEAMYSELGINLTTAINVFLRQSLRV
                     GGFPFDVRIEKPNKETIAAMLEAERIAHDSSVKRYSDVEEALKALKE"
     gene            431..718
                     /locus_tag="ACZPDN_06935"
     CDS             431..718
                     /locus_tag="ACZPDN_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HIR1298392.1"
                     /translation="MSKHLDIVWTNQFKKDYKLAMKHHLDIDLLDNIIRKLASNEELP
                     EKNKDHALTGNWIGHRECHIQPDWLLIYQIENDLLVLTLTRTGTHSDLFGK"
     gene            complement(822..992)
                     /locus_tag="ACZPDN_06940"
     CDS             complement(822..992)
                     /locus_tag="ACZPDN_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298393.1"
                     /translation="MAIKSYTDTYKIKGSDVDRFCRIMSDAKKIEIKKVNEHHDLKDV
                     EEFKKMFGFVKK"
     gene            complement(1340..1684)
                     /locus_tag="ACZPDN_06945"
     CDS             complement(1340..1684)
                     /locus_tag="ACZPDN_06945"
                     /inference="COORDINATES: protein motif:HMM:NF025852.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1298394.1"
                     /translation="MVVVDKENFMGGTGIAHFEHVMMADEMHGMNRVYAKVTLKKGCS
                     VGYHVHNGDGEDYFVLSGMATLDDNHERTLYLKPGEHYFTPSGKGHSISNEQDEDLVF
                     MALIIYDSQQKI"
     gene            complement(1696..2754)
                     /gene="trpS"
                     /locus_tag="ACZPDN_06950"
     CDS             complement(1696..2754)
                     /gene="trpS"
                     /locus_tag="ACZPDN_06950"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1298395.1"
                     /translation="MAKIILTGDRPTGKLHIGHYVGSLRNRVLLQNSGEYDEIYIMIA
                     DAQALTDNFDNPDKVRENILEVALDYLACGIDPSKTTIFIQSEISQLTELTFYYMNLV
                     SVSRLQRNPTVKAEIKMRGFDASIPVGFQCYPISQAADITAFKATTVPAGEDQMPMIE
                     QTQEIVHKFNSIYGETLVEPKILLPQNDACLRLPGTDGKAKMSKSLGNCIYLSDDEET
                     IKKKVMSMYTDPDHIKVSDPGKIEGNCVFTYLDAFCKPEHFEKYLPEYENLDALKAHY
                     QRGGLGDVKVKRFLNSVMQEELAPIRERRLEWQAKLPEVVKILEEGTKKAYATAEKTL
                     NEVKSSMKINYFKDNSLI"
     gene            complement(2767..3768)
                     /gene="galE"
                     /locus_tag="ACZPDN_06955"
     CDS             complement(2767..3768)
                     /gene="galE"
                     /locus_tag="ACZPDN_06955"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1298396.1"
                     /translation="MNILIAGGTGYIGSHICVELLESGHDVVVIDDFSNSKPDVLDSI
                     YKITGKHVKFYEFNVLEEEKTEAVFKENKLDAVIHCCAFKAVGESVQKPIEYYTNNLM
                     TTLVVAKMMKKYHVPSIVFSSSATVYGDPEVVPLTEDCKLGETTNPYGATKAMMERIL
                     TDVQFANPEMSVTLLRYFNPIGAHESGLIGENPKGIPNNLMPYIMKVATGELKELGVF
                     GNDYDTPDGTGVRDYIHVVDLAKGHVLAIEKYNTPGVHICNLGTGKGYSVLDIVHAFE
                     RVNGIKIPYVIKPRRAGDIATCYADPTRAKVQLGWVAEKNLEDMCRDTWNFAKKQLL"
     gene            complement(3801..4283)
                     /locus_tag="ACZPDN_06960"
     CDS             complement(3801..4283)
                     /locus_tag="ACZPDN_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HIR1298397.1"
                     /translation="MKERMIPVTCPHCGHVFEIKRDTVVIAQMDHVARSRLDDGSYFM
                     HQCQNCKSMFYLYYPFLYRDPKKKFNLVLTEQKNIDNLCEDEQVVLCHSVSQFLLAFK
                     IYDQCLDPKMVLVKKKQLEKKLNRSVKFDYFDMKNHCLWFEDKAVSLTEKECKEILIL
                     "
     gene            complement(4283..6061)
                     /locus_tag="ACZPDN_06965"
     CDS             complement(4283..6061)
                     /locus_tag="ACZPDN_06965"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR1298398.1"
                     /translation="MIKERISKLRAKMMENNVQAYIVPTSDFHETEYVCDYFACRKYM
                     SGFTGSAGVLVVLLDKAALWTDGRYFIQAASQLEGSGIDLMKMGQPGVPEIDAYIVEN
                     LKENDTVGFDGRVMNTKDALAYKSVFDLAHLNMNVNLDLVNEVWTDRPQLPSTPTFHY
                     SEKFSGESVESKLSRLRAFLKENKVDSIVLTSVDQIAWLYNLRAHDIPNFPVALAYSI
                     VSLESASIYMDASRLDELSLKEFKDNHVTVCGYSDIYLATKALTGLVLVDPSSVNYAI
                     VSNLQAKPVFKESPIILWKALKNDTELKCTKWAHIKDGVAVTKFMYWLKKNAGKIEMS
                     EMSVQSKLQLLRSEQENYLEDSFDTICAYKEHAAMMHYSSKPETNVDITNSGMLLIDS
                     GGQYLEGTTDITRTFVLGDISEEERYWFTKALRGHIRLSDAHFLFGCSGINLDILARG
                     PLWDQDVDYQCGTGHGVGHLLNVHESPNGFRWRVLPHRNEMCVLDEGMITSNEPGVYC
                     EGKFGIRHENEMVVVKGNVNNYGQFMHFEPITFVPFDLDGLDVSLLTNEEKTWLNNYH
                     QEVFEKISPYLTRDEAEWLKSACRSI"
     gene            complement(6080..6778)
                     /locus_tag="ACZPDN_06970"
     CDS             complement(6080..6778)
                     /locus_tag="ACZPDN_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298399.1"
                     /translation="MGDYIVMGIVGILILVMSVLPKTVYNGITYTFSMHKYGIRKIQR
                     YRTTTDTLANCIIGVLVVFSIFYCFIPFYSVVYTILFILSYLCLLAQVNRVTSKKTQQ
                     VARTVILLNNIFAGVCFLGALGFMNGHMADGVINQFMLDFHAHKVFGILYLLQNRTWM
                     YWLFQGILFLFPLFIMWSHFKYMRLENSVKAVYFITYILKMLFLIIVVVCFSVGAFEF
                     LDKVYQVDALKKLA"
     gene            6857..7690
                     /locus_tag="ACZPDN_06975"
     CDS             6857..7690
                     /locus_tag="ACZPDN_06975"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1298400.1"
                     /translation="MKLVFLDADGTLFHHEGYIPDSAIKACIQAQKNGHKIILCTGRQ
                     RIEIFGPMLEIDYDAIIAGSGATIECEHKIIEENALSKEESQYLSNYLLKNNIPANFE
                     SSNGIYATQYTKDTMLKMVEEQCAGKTQEEKAKHGLTILTSQITVVDNIQDLIYNKVS
                     VIDNGKTPYKKIQQDLSDKYDVIPATFAPLGKESGEVTSYHINKATGMDAVIKYFHAD
                     IKDTIAIGDGFNDLPMFKKAHLSIAMGNAPQEIKDKADRITTSLDEDGIYRAFKDLNL
                     I"
     gene            complement(8055..>8438)
                     /locus_tag="ACZPDN_06980"
     CDS             complement(8055..>8438)
                     /locus_tag="ACZPDN_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HIR1298401.1"
                     /translation="ILNDKCGMDTALPEEISKRMKELLKEYNDKKEKSFEDILDFHVR
                     FERIHPFQDGNGRVGRLILFKECLKYNIVPFIIDDHLKMFYYRGLKEWDYEKGYLIDT
                     CLSAQDKYKAYLDYFRIAYENNKSN"
BASE COUNT         2860 a         1755 c         1134 g         2689 t
ORIGIN      
        1 gatagttaca tttattattc tcgcatcaaa tatagtcatt agtgtattcc tacaaaataa
       61 ttcagttttt ttgtatataa tatatatcta ttagattgac tatgcattgc attagatgta
      121 caatttaagt gtaataaata gataaggagt tgacatttat ggctaataca actaatttta
      181 gtgtccgtat ggacagtgat attaagaaac aatgcgaagc tatgtatagc gaattaggta
      241 tcaatcttac tactgctatt aatgtatttc ttcgtcaatc acttcgtgta ggcggttttc
      301 cttttgatgt tcgtatagag aaaccaaata aagaaacaat agcagcaatg ttggaagcag
      361 aacgcattgc acatgattca tctgtaaagc gatactctga tgtagaggaa gcattaaagg
      421 cattaaaaga atgagcaagc atttagacat cgtttggaca aatcaattta agaaggatta
      481 taagcttgcg atgaagcatc atcttgatat cgaccttctt gacaacatta ttcgtaaatt
      541 agctagcaac gaagaacttc cagagaagaa taaagatcac gcattaactg gaaactggat
      601 tggacatcgt gaatgtcaca ttcagccaga ttggctacta atctatcaaa ttgaaaatga
      661 tttactcgtg ttaacattaa cccgaacagg aacacatagc gatttgtttg gaaagtaaaa
      721 aggagcactc ggtattactt catcaaaata ccgggtgctt tttactttat gcaacaataa
      781 aaattaaata atccaaaacg attatccgta aatgaccact atcatttttt tacaaatcca
      841 aacatttttt tgaattcttc aacatctttt agatcatgat gctcatttac ttttttgatt
      901 tcaatttttt ttgcatccga cataatacga caaaatcgat ctacatcaga accttttatt
      961 ttgtatgtat ctgtataact cttaattgcc atacaatata cctaaccttt catttataca
     1021 ttaaataaag aacgcctgcc cggtgatgct tacccgggta catttttttc cattaagacg
     1081 tataagacac atcttttacc cacttcagaa gttcctatat ctagtataca caaagtttaa
     1141 ccaacaaaaa agagtaggcg cgcattccta ctctttatgt atcctccatt aagtcgcgat
     1201 ttaagagaat atactgacgg attatttctc acagaaatat tatagagaga aatagttcta
     1261 tgtgcgaaaa cgctttcaca aaaccaatag tattatatat caaatcggct ccttgcgttt
     1321 gcacaaatta gtaatctatt tatatttttt gttgagaatc ataaataatc aaagccatga
     1381 aaaccaaatc ctcatcttgc tcatttgaaa tactatgacc cttaccactt ggtgtaaaat
     1441 aatgttcacc tggttttaaa tataaagttc tttcatgatt atcatcaagc gttgccattc
     1501 cagaaagaac gaaataatct tcgccatctc cattatgcac atgataacca acactacacc
     1561 ccttctttaa agtgaccttg gcatagacac gattcatacc atgcatctca tctgccatca
     1621 tgacatgttc aaaatgcgca ataccagtac cacccataaa attttcttta tcaactacta
     1681 ccattttcta tacccctaga tcaaagaatt atctttaaaa taattgatct tcatacttga
     1741 tttcacttca ttcaacgtct tttcagccgt cgcataagcc ttttttgtac cctcttctaa
     1801 aatcttgaca acctcaggaa gttttgcctg ccactccaat cttctttccc gaattggggc
     1861 caattcctct tgcataacac tatttaagaa acgtttcact ttcacatccc ctaaaccgcc
     1921 acgttgataa tgtgctttta atgcatccaa gttttcatat tccggcaaat acttttcaaa
     1981 atgctccggc ttacagaacg catccaaata agtaaataca caatttccct caatcttacc
     2041 aggatcactc accttgatat gatctggatc tgtatacata ctcataactt tctttttaat
     2101 tgtttcctca tcatctgata aataaataca attacccaat gatttagaca tcttcgcctt
     2161 accatccgta ccaggaagac gtaaacaagc atcgttttgt ggcaataaga tcttaggctc
     2221 aactaaagtc tcaccataaa tcgaattgaa tttatgaacg atctcctgtg tttgttcaat
     2281 cataggcatc tgatcttctc cagcaggaac ggtcgtagcc ttaaatgccg taatatcagc
     2341 agcctgtgaa attggataac actggaatcc tacaggaata ctcgcatcaa atccacgcat
     2401 tttaatttca gcctttaccg ttggatttct ttgaagacga cttacagaaa ccaagttcat
     2461 ataatagaat gtcaattctg ttaattgact aatttcagac tgaataaaaa tcgttgtctt
     2521 actaggatca ataccacaag ccaaataatc taaggcaacc tctaaaatgt tttcacgaac
     2581 cttatctgga ttatcaaagt tatctgtcaa agcctgcgca tccgcaatca taatataaat
     2641 ctcatcatat tccccggaat tctgtaaaag aacacgattt cttaatgaac caacataatg
     2701 tccaatatgt aattttcctg tgggacgatc ccccgttaaa ataatctttg ccataaatac
     2761 ctcattctac aataattgtt tcttcgcaaa attccatgta tctctacaca tatcctctag
     2821 attcttttca gctacccagc caagctgaac ctttgctctt gtaggatctg catagcaagt
     2881 cgcaatatca cctgcacgtc ttggtttgat cacataagga atcttaatac cattcactct
     2941 ttcaaacgca tgtacaatat ctaatacact ataccctttt cctgttccca agttacaaat
     3001 atgaacacca ggcgtattgt atttttcaat cgctagtaca tgtccttttg ccaagtcgac
     3061 cacatgaata tagtcacgaa cacccgttcc atctggcgta tcataatcat tgccaaatac
     3121 accaagttct ttaagctcac cagtcgcaac cttcataata taaggcatca agttatttgg
     3181 aattcccttt ggattttcac caatcaatcc cgattcatgc gctccaattg gattgaaata
     3241 acgaagtaaa gttacactca tttctggatt tgcgaattgc acatccgtta aaattctttc
     3301 catcatcgcc tttgtagctc catatggatt cgtagtttca cctaacttac aatcctctgt
     3361 taaaggaacc acttcaggat ctccataaac agtcgcactt gaactaaaca caatacttgg
     3421 aacatgatac tttttcatca tcttggcaac cactaaagtt gtcatcaagt tatttgtata
     3481 atactcaatt ggtttttgaa cactttcacc aaccgcttta aatgcgcaac aatgaatgac
     3541 cgcatccaat ttattttcct taaatacagc ctctgttttt tcttcttcta acacattaaa
     3601 ttcataaaac ttaacatgtt ttcctgtaat cttatagatt gaatccaata catctggttt
     3661 agaattggag aaatcatcga tcacgacaac atcatgacca ctctctaata attcaacaca
     3721 aatatgacta ccaatatatc cggttccacc cgcaattaaa atattcatag ttaaaaacct
     3781 catttctatc tgctattata ctacaaaata agaatctctt tacattcttt ttctgttaaa
     3841 gatacggctt tatcctcaaa ccaaagacaa tgatttttca tatcaaaata atcaaactta
     3901 acacttcgat ttaatttctt ctctaactgt ttctttttaa ccaataccat ctttggatct
     3961 aaacactgat catagatttt aaaggctaaa agaaactgtg aaacactatg acacaataca
     4021 acttgctcat cttcacatag attatctata tttttttgtt cggttaagac aagattaaac
     4081 ttctttttcg gatccctgta aagaaatgga tagtacaaat aaaacataga cttacaattt
     4141 tgacattgat gcataaaata agaaccatca tctagtcgac ttcttgccac atgatccatt
     4201 tgtgcaatca caaccgtatc tctttttatc tcaaaaacat gcccacaatg tggacatgtt
     4261 accggaatca ttctttcttt cattaaatcg atctacaagc ggattttaac cattcagcct
     4321 catcccttgt taaataagga ctgatctttt caaatacttc ttgatgatag ttgtttaacc
     4381 aagtcttttc ttcatttgtc aataaagaaa catccaaacc atctaaatca aatggtacaa
     4441 atgtgattgg ttcaaagtgc atgaattgac cataattatt tacattgccc ttaacaacaa
     4501 ccatttcatt ttcatgacga ataccaaatt taccttcaca ataaacaccc ggctcatttg
     4561 aagtaatcat accctcatct aatacgcaca tttcattacg atgtggcaat actctccatc
     4621 taaatccatt tggcgattcg tgtacattca acaagtgacc aacaccatgt cctgtaccac
     4681 attgataatc cacatcttga tcccataagg gcccacgagc tagaatatct aaattgattc
     4741 ctgaacatcc aaataagaag tgtgcatccg ataaacgaat gtgtccacgc aatgcttttg
     4801 tgaaccaata tctttcttct tcactaatat cacccaacac aaatgtacgt gtaatatctg
     4861 ttgtaccctc taaatattgt ccaccactat caattagaag catacccgaa tttgtgatat
     4921 caacatttgt ttctggcttt gaagaatagt gcatcatagc tgcatgttct ttataagcac
     4981 aaatcgtatc aaaactatcc tctaaataat tttcttgttc actacgaaga agttgcaatt
     5041 tagattgaac actcatctcg ctcatttcaa tcttacctgc attcttcttt agccaataca
     5101 tgaatttagt aacggcaaca ccatctttaa tatgtgccca ctttgtacat tttaattccg
     5161 tatcattctt taaagccttc cataaaatga ttggactttc cttaaatacc ggcttagcct
     5221 gcaagtttga aacaatcgca taatttacac tggatggatc gactaagact aaacccgtta
     5281 aagcttttgt tgccaaatat atatccgaat agccacaaac tgtaacatgg ttgtctttaa
     5341 attcttttaa actcaattca tccaaacgag atgcatccat atagatggat gctgattcaa
     5401 gtgaaacaat cgaataagct aaagcaactg gaaaattagg aatatcatgc gctcttaaat
     5461 tataaagcca tgcgatctga tccacacttg ttagtacaat agaatcaact ttgttttcct
     5521 taaggaaagc acgtaaacga cttaacttac tctctacaga ttcacctgag aatttttcag
     5581 aataatgaaa agttggcgta ctaggcagtt gaggacgatc tgtccatact tcattgacta
     5641 agtctagatt tacattcata ttcaaatgtg ctaaatcaaa taccgactta taagctagag
     5701 catcctttgt attcatgaca cgaccatcaa agccaactgt atcattttct ttaagatttt
     5761 caacaatata cgcatcaatt tcaggaactc caggttggcc catcttcatt aaatcaatgc
     5821 ctgagccttc aagctgactt gcagcctgaa taaaataacg tccatctgtc cataaggcag
     5881 ccttatcaag caatacaact aaaactcctg cactccctgt aaagccagac atatatttac
     5941 gacatgcaaa gtaatcgcaa acatattccg tttcatggaa atcacttgtt ggaacaatat
     6001 aagcctgtac attgttctcc atcatttttg cacgcaattt tgaaattctt tctttaatca
     6061 tttataaatc cttctttctc tatgccaatt tctttagggc atccacctga taaactttat
     6121 ccaagaattc aaaggcaccc acagaaaaac atacaaccac aataattaaa aataacattt
     6181 ttagaatata tgttataaag taaacagcct ttacactgtt ttctaaacgc atatatttaa
     6241 aatgcgacca catgataaat agtgggaata aaaataatat gccttgaaac aaccaataca
     6301 tccaagttcg attttgtaga aggtataaaa taccaaatac tttatgcgca tgaaaatcca
     6361 acatgaattg attgatcaca ccatctgcca tatgtccatt cataaagcca agcgctccta
     6421 aaaaacaaac tcccgcaaaa atattattca ataaaatcac agttcgagcg acttgttgtg
     6481 tcttttttga cgtaacacga ttcacttgtg caagcaggca caaatagctc aatataaata
     6541 gaatcgtata tactacggaa tagaatggaa tgaaacaata aaaaatacta aagacaacca
     6601 gtacacctat aatgcaattt gctaacgtat cggttgtggt gcgatatctt tggatttttc
     6661 gaattccata tttatgcatt gaaaatgtat atgtaatccc attataaacc gttttaggta
     6721 aaaccgacat tacaaggatt aatataccaa caatgcccat cacaatataa tcacccattg
     6781 taccctcccc tttaaatatt tacctatcta ttgtagaata aaaatatcat ttcgtccaat
     6841 aagaaagagg tgtagaatga aacttgtttt tttagatgcc gatggcacac tatttcatca
     6901 cgaaggctac attccagata gcgcaattaa agcttgtatt caagcacaaa aaaacggtca
     6961 taaaatcatt ttatgcacag gaagacaacg cattgaaatc tttggtccca tgttagaaat
     7021 cgactacgat gcaatcattg ccggaagtgg tgcaaccatt gaatgcgaac ataaaattat
     7081 cgaagaaaat gcattatcta aagaagaaag tcaatattta tcaaactatc ttcttaaaaa
     7141 taacattcca gctaactttg aaagcagtaa cggcatctat gccacgcaat atacaaaaga
     7201 tacaatgtta aaaatggtcg aagaacaatg tgcgggtaaa actcaagaag aaaaggccaa
     7261 acatggcctt acgattctta caagtcaaat tacagttgtc gacaacattc aagacttaat
     7321 ctacaataaa gtatctgtca tcgacaatgg aaaaacaccc tataaaaaga tccaacaaga
     7381 tctttcggat aaatatgatg taattcctgc cacatttgcg cctttaggaa aagaaagtgg
     7441 tgaagtcaca tcctatcata tcaataaagc aacaggcatg gatgccgtaa tcaaatactt
     7501 tcatgccgac atcaaagata ctattgcgat tggagatggt tttaatgatc ttcctatgtt
     7561 taaaaaagca catctaagca ttgccatggg taatgcacca caagaaatca aagataaagc
     7621 agataggatc acaacatccc tagatgagga tggtatttat cgtgccttta aagatttgaa
     7681 tctgatttaa tgcaaagctc tttcaagggc tttttctttt acctgaatca tatttgatta
     7741 tggttgtaag taaatctgaa gatcttttct tgattacaca aacagaacac tacatcccta
     7801 attttgagca tgattagaaa cactcaaaag aaggcaggac actcctgaaa gcgccctgcc
     7861 tgtatattat agcgtgtact ctaagttatt ttacaagttt aaaacattct acagaataga
     7921 tttttgtaag atattgcatt tttctattct aaataagagc tttaatcatg aaaatatact
     7981 tcttttccaa caaaaaatcc attttgattt aattgtgata ctctttccct tcaatcacaa
     8041 tataatcatc gatttcaatt tgatttatta ttttcataag caatcctaaa ataatccaaa
     8101 taggctttat atttatcttg cgccgataga caagtatcta tcaaataccc tttttcataa
     8161 tcccactctt ttaaaccacg ataataaaac attttcaaat gatcatcgat aataaaggga
     8221 actatattat atttcaaaca ttccttgaat aagatcaatc ttccaacacg accatttcca
     8281 tcttgaaatg gatggatacg ctcaaatctt acatgaaaat cgagtatatc ttcaaatgac
     8341 ttttcttttt tatcattata ttcttttaac aattccttca tccgtttgga aatctcttca
     8401 ggtagtgctg tatccattcc acacttatca tttagtat
//
LOCUS       DBMDWL010000087         8225 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3184_length_8225_cov_2.489783, whole genome shotgun sequence.
ACCESSION   DBMDWL010000087 DBMDWL010000000
VERSION     DBMDWL010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3184_length_8225_cov_2.489783"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="ACZPDN_06985"
     CDS             <1..476
                     /locus_tag="ACZPDN_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIR1298402.1"
                     /translation="HVPKIHHSLAWCYLLNCQYSECIKYTNLAIENKDPSCDLCYFIP
                     YSYYKQNEYLKCLDYIESHLEFVDDFYKPFLQAISARIQKQNVDFEKNIILYYQSLLQ
                     NNVYEDIPLIQNFILDYYTETNNKDMMIKILIDIKSFNDKDLTLKSSTLFTTSQS"
     gene            complement(529..1812)
                     /locus_tag="ACZPDN_06990"
     CDS             complement(529..1812)
                     /locus_tag="ACZPDN_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015397.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIR1298403.1"
                     /translation="MATTKIWSVKNRLDHVLEYVSNEDKTIDKLGSYVSRNEATENQK
                     YMTCVNCSGNDPFTSMMNLKDVFHDTSNIIAYHAIQSFKPHETNADTVHEIGVETATR
                     LYGDRYQFVVCTHLDKDHLHNHIVLNPISIVDGKRYHNSMKDIYQLREVSDEICKEYG
                     LSIVEKPQGKGQSRSQYYSARSYIREIKKDIDAACIRAFTVKDFVQDMKLEGYSFEEV
                     EGLDCVVHPNYDKPIPLLLLGQRYSLDFIEQRIEKEEVRYVPLKIIYKQKDADRIYGQ
                     YKSGMFKGFMKEVVKFQVLLGILPNYSATKIKLSKEMRKECRKLDLITNATTIMAKND
                     IDTLEKLESYVDLLDKQMNGLLAQRKHLYSRSYKEKDLIAKAKLSKEAKALTPDIKEL
                     RSQIKSLDYIRKRSTKYREYNKQLEQKRNLDKQRA"
     gene            complement(1799..2215)
                     /locus_tag="ACZPDN_06995"
     CDS             complement(1799..2215)
                     /locus_tag="ACZPDN_06995"
                     /inference="COORDINATES: protein motif:HMM:NF039635.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIR1298404.1"
                     /translation="MIWQNNKENVLASNAYGHPYTEGRSSNMTRKREHRMTVRLNGDE
                     YTKVLSKIERSGLSKQEYILSSILSKKIQERLNVDYFKLINEVNHVGTNLNQIARKLN
                     GNESILKDEIFKNQLLLNQVLKNLNDQIKGNSNGNN"
     gene            complement(2445..3881)
                     /locus_tag="ACZPDN_07000"
     CDS             complement(2445..3881)
                     /locus_tag="ACZPDN_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HIR1298405.1"
                     /translation="MSLLDEIVVNIGSIVCKDELKEYLFRNACFVNDEDMYVIETALS
                     QREDIDARWRKSIYAQLLKSPKEGFIVNMILQEWNLRYLKGRGFYLYTGKYWKYTQDE
                     IVSKKIGDLLGKWKQGNKVRSILKLLKDECITEEEFNKKDVICFQNGTLHLESGLLKK
                     HSSKDLCSIIMSYDYDETKESKEWAAFVMDICNGKQYRYDKLQLLCGYILMSDCHLQK
                     CFIFYGTGANGKSVFMNIISNVFGQENVTHLQLLDMYDKFQLIQLESALLNLGEDSGS
                     SLKGGDSELKRLSAGDRVSACYKGKDFISFRSRAKLIFAMNDILFSSAINQGILRRLS
                     TIMFEVHFVDDPKLPEEKKIDRNLEDKLSKELSGIFNWCYEGYLKLKDVDCFARDEDD
                     IEMERMFLDVSSPLYGYYVQMEPLKRFTPTREIYDNYLFWCRDKGIKPKALSTFSSQF
                     ALVSRSSYKKNDSYKDENGKHIRGYEPL"
     gene            complement(3865..4248)
                     /locus_tag="ACZPDN_07005"
     CDS             complement(3865..4248)
                     /locus_tag="ACZPDN_07005"
                     /inference="COORDINATES: protein motif:HMM:NF046164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIR1298406.1"
                     /translation="MPGLNKNRKRPVTIAFRVTAQEAQRINQRVKISGLSRRKYFMNT
                     FLGQKIELRGGCFESNRLASELKKIRMRLEVLEVNSPQMEETIDECYALLSELSKVIK
                     ENEPVQKEVPAFTQEEDWLEDVIIR"
     gene            complement(4249..5316)
                     /locus_tag="ACZPDN_07010"
     CDS             complement(4249..5316)
                     /locus_tag="ACZPDN_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1298407.1"
                     /translation="MASITLNPKTKKYDVQFRVKDAMGNCKKSTKRGFKRKSDAKKWL
                     NEYELKKSENLSMKFSDYVDVYLEDLQPRLRKTTYETKRTYLRVHITPFFGNYGLDDI
                     SVSMVRKWQNQLIERGYSETYQKTLNNQLSALFNHAVAYYGLKNNPVRIAGPIGKEHG
                     DEVEFYTKEEFDRLIEQTMNNQTVYTIFMTFYWTGMRLGELRALTPNDIRIDEKYIDV
                     NKSLQVVKGKEIITDPKTPKSKRKITIPDFLATILKEYMDSLYNPNPNKPIFRMSKGA
                     IHHALKTSAVKAGLKPLRIHAFRHAHAAYLIEMQVPILAISNRLGHEKIQTTMNTYGH
                     LYPNKQRQIADAIEARVKGEF"
     gene            complement(5322..5525)
                     /locus_tag="ACZPDN_07015"
     CDS             complement(5322..5525)
                     /locus_tag="ACZPDN_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ICEBs1 excisionase"
                     /protein_id="HIR1298408.1"
                     /translation="MSSKALLGAADIAEILGVSKSKAYTIIREGNQELKSMHKLVISG
                     KLPVEYFNDICYSSKSNRDEEGS"
     gene            5724..6389
                     /locus_tag="ACZPDN_07020"
     CDS             5724..6389
                     /locus_tag="ACZPDN_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1298409.1"
                     /translation="METMGQRIKKTRKMMKMTQVEFAKLLDVPQPSISAYEKDKYLPS
                     FEVIGKIMKECNVSFEWLNGYNSVPDSTLTVTDIGNMISQLFEIDSLSFQVEAEMSHS
                     SSKDKSECIHPKVTFTLLNNDALGDNLISYLYEMQSVCFDYSNHLISEEESLNKKSQI
                     YEKYMIPISFKKYRETSEKELMETQVKALQKKLNNEKESNNSEEKFTVITGNINNLND
                     KNE"
     gene            complement(6450..6770)
                     /locus_tag="ACZPDN_07025"
     CDS             complement(6450..6770)
                     /locus_tag="ACZPDN_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298410.1"
                     /translation="MKKLRLLIPLFIGLVAVTAYPAFARSSEEPVNVEKVDEANEDLG
                     YFPGCDDDEKVIPLGDGFLGGEAYLQNGDEEIYLNSETDSGAITVKEAKEILKSRQAE
                     SDNQ"
     gene            complement(6787..7437)
                     /locus_tag="ACZPDN_07030"
     CDS             complement(6787..7437)
                     /locus_tag="ACZPDN_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298411.1"
                     /translation="MKNKTLLAVALVAALGFTTCSVFAEELDTKPKSHENTEVLFGDL
                     NDNDKVVELEDGGFLYGEATITDENDPTFEPVEYNSFDDPNSITVKEAKKIILDGSAS
                     NFARGSNVPDSSTKILILKPNSHYQSSEFSGTGWRFGGYFFKASSGSGTYLRWTSYKD
                     AGRVGNYSEAVGTKNGSLQGTVIGKNQTQYVSRGGSGMIYYTYSPIQGTYYYVVNI"
     gene            7565..7924
                     /locus_tag="ACZPDN_07035"
     CDS             7565..7924
                     /locus_tag="ACZPDN_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1298412.1"
                     /translation="MEQLTKKERTIMDILWNATSSLSASEIKDTSPDLNINTVQQVLR
                     NLLKCKFIEVDDIVFNKKSLTRTYKPVVSQAQYISAFISDSNRFKLLCGLVDEEDDLD
                     SLNELQKLINKRKKEIR"
     gene            7930..>8225
                     /locus_tag="ACZPDN_07040"
     CDS             7930..>8225
                     /locus_tag="ACZPDN_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298413.1"
                     /translation="MTFILSTLLSTLILTSCMIFIFDFCIHKQWNMNQVFFKLFLLII
                     FIKLCFPFETKLSILIPLDKIMNPLIDILQYKLFNQIYMYHFLLSVAVIGSIYQ"
BASE COUNT         2575 a         1646 c         1075 g         2929 t
ORIGIN      
        1 aacatgttcc aaaaatacac cacagtctag cttggtgcta tctattaaac tgtcaataca
       61 gtgaatgtat aaaatacaca aacttggcaa tcgaaaacaa agatccatct tgtgatttat
      121 gttatttcat accctattca tattacaaac agaatgagta tctaaaatgt ttagattata
      181 tagaatctca tctagaattt gtagacgatt tttacaagcc ttttttgcaa gctataagtg
      241 ctcgtattca aaaacaaaat gttgattttg aaaaaaacat cattttgtat tatcagtcac
      301 tcttgcagaa caatgtatac gaagatattc cacttattca aaactttatt ttggattact
      361 atacagaaac caacaataaa gatatgatga tcaaaatcct tattgacatc aaatcattta
      421 acgataaaga tttaacatta aaaagctcta cactctttac cacttcacaa agttaagaaa
      481 atcgcactta tttatcatta attaaatctt gggtaaatcc ttatttactc aagctctttg
      541 tttatctaaa ttccttttct gttctaactg tttattatac tctctatact ttgttgaacg
      601 ttttcttata tagtctagac tcttgatctg acttcttaat tctttaatat ccggtgttag
      661 tgcttttgct tctttggata gttttgcctt ggcaattaga tccttttctt tgtaggatct
      721 ggaatataag tgctttcttt gtgccagtaa tccattcatc tgtttatcaa gtaagtctac
      781 ataggattct agtttttcta atgtatcaat atcatttttt gccatgatcg ttgttgcatt
      841 tgtgataaga tcgagctttc tacattcttt tcgcatttct tttgaaagct ttattttcgt
      901 tgcagaataa ttcggaagaa tacctaacaa cacttgaaat ttaaccactt ctttcataaa
      961 gcctttaaac attccacttt tatattgtcc ataaatacga tctgcatctt tttgtttgta
     1021 aataatcttt aagggaacat atctaacttc ttctttttct attctctgtt caataaaatc
     1081 caaagaatat ctttgtccca gcaacaacaa tggtattggc ttatcataat taggatgaac
     1141 tacgcagtcc agtccttcca cttcttcaaa tgaatatcct tccagcttca tatcttgaac
     1201 aaaatctttg actgtaaatg cacgtataca ggcagcatct atgtcttttt ttatttctcg
     1261 aatatagctt ctagccgaat aatactgact tctagattgt ccttttcctt gtggcttttc
     1321 cactatactc aatccgtatt ctttacatat ttcatctgaa acttctctta actgataaat
     1381 gtctttcatc gaattatgat atcttttgcc atcaacaatc gatatcggat tcaatacaat
     1441 atgattatgc agatgatcct tatccaaatg tgtgcatacg acaaactgat atctatctcc
     1501 atataatctt gttgcggttt caacaccaat ttcatgaact gtatctgcat ttgtttcgtg
     1561 tggcttaaat gattgtatcg catgatatgc aataatattg gatgtatcgt gaaaaacatc
     1621 ttttaaattc atcatacttg taaatggatc attgccagaa cagttcacac aagtcatata
     1681 tttctgattt tctgtagctt catttcttga aacatacgat ccaagtttat cgatcgtctt
     1741 atcttcatta gatacatatt caagaacatg atccaatcga tttttaacac tccatatctt
     1801 agttgttgcc atttgaattc cctttgatct gatcattaag attcttcaaa acctgattga
     1861 gcaacagctg atttttaaag atttcatcct tcaaaataga ttcattacca tttaattttc
     1921 ttgcgatctg atttagattt gttcccacat gatttacttc atttattaat ttaaaataat
     1981 ccacattcaa tctttcctga atctttttac tcagaatcga agataagata tactcctgtt
     2041 tggataatcc agacctttca atcttactca aaactttagt atattcatcc ccattcaaac
     2101 gcaccgtcat tcgatgttct cgttttcgcg tcatatttga tgacctccct tctgtatatg
     2161 ggtgaccata tgcgttgctt gctaatacat tttccttatt attctgccaa ataatttatg
     2221 acgatatgac gattggaagg gtatatcccc aaaaacgtca caccgtcatt gtcaaagtgc
     2281 ttcatcttct atttcattta cctggcttta ctgattctcg catttcttct gtcttctacc
     2341 taccgaaaaa aaatacctgt cgtttgaaaa ataaccaggt acactggttc taatacatat
     2401 caaaatcaga accgggtaac ctggttaatt taaacatagt gattttataa cggttcatat
     2461 cctcgaatat gttttccatt ctcatcttta taagaatcat tctttttata agaacttctt
     2521 gaaaccagtg caaactgtga tgagaatgtt gatagtgctt taggtttaat tcctttatct
     2581 ctacaccaaa ataaatagtt atcatagatt tctctagtag gagtaaatct ttttaacggt
     2641 tccatttgaa cataatatcc atacaatggg gaagatacat ccaaaaacat tcgttccatt
     2701 tcaatatcat cttcatctct agcgaaacaa tcaacgtcct tcaattttaa atatccttca
     2761 taacaccaat tgaaaatgcc agaaagctct ttagaaagtt tatcctcaag attgcgatcg
     2821 atcttttttt cttcgggcag tttcggatca tctacaaagt gaacctcgaa cataatcgta
     2881 gacagtctac gcaatatccc ttgattgatt gcacttgaaa acaagatatc gttcatcgca
     2941 aaaatcaatt ttgctcttga acgaaaacta atgaagtcct ttcccttata gcatgcacta
     3001 acacgatctc cggcagataa tctttttaat tctgaatctc caccttttaa agaagaacct
     3061 gaatcctcac ctagatttaa tagtgcagac tccagctgaa tcagttgaaa cttgtcgtac
     3121 atatctaaaa gctgcagatg tgtcacattc tcctggccaa atacatttga aataatattc
     3181 ataaatacgc tcttaccatt tgctcctgta ccataaaaga taaaacattt ctgcagatga
     3241 caatcactca tcaaaatata tccacataac aattgaagtt tgtcataacg atattgtttt
     3301 ccattacata tatccatcac aaatgcagcc cattcttttg actctttggt ttcatcgtaa
     3361 tcataagaca ttatgattga acaaagatcc ttagaactat gtttcttcaa caaacccgat
     3421 tcaagatgca gcgtaccatt ttgaaaacat ataacatctt ttttattgaa ttcctcttct
     3481 gttatacatt catctttcaa caacttcaaa attgaacgca ccttgtttcc ctgtttccat
     3541 ttccccaata aatcaccaat ctttttagaa acaatttcat cttgcgtata cttccaatat
     3601 ttacctgtat aaagatagaa tccacgaccc tttaaatagc gaagattcca ttcctgcaat
     3661 atcatattta caataaatcc ctcttttgga gattttaaaa gctgtgcata aatagacttt
     3721 ctccatcgag catctatatc ttccctttga gaaagtgctg tttcaatcac atacatatct
     3781 tcatcattga cgaaacacgc gtttcgaaat aaatattcct tcaactcatc tttacacaca
     3841 atacttccta tattcactac aatttcatct aataatgaca tcttctaacc agtcttcttc
     3901 ttgagtgaat gcaggaactt ctttttgtac aggctcattt tctttgatca ctttcgatag
     3961 ttcagaaagc agagcataac actcatcaat cgtttcttcc atttgtggtg aatttacttc
     4021 cagcacctct agcctcattc gaatcttttt cagttctgaa gcaagccgat tactttcaaa
     4081 acagcctcct cttagctcaa tcttttggcc tagaaaagta ttcataaagt attttcttct
     4141 tgataatccc gaaatcttaa ctctctgatt gattctttgt gcttcctgtg cagttactcg
     4201 aaaagcaatt gtgacaggtc ttttgcgatt cttatttaat cctggcatct aaaattcacc
     4261 ttttaccctt gcttcaatcg catctgcaat ttgtctttgt ttatttggat ataaatgtcc
     4321 ataagtattc atggttgtct gtatcttttc gtgtcccaac cgatttgaga ttgccaaaat
     4381 tggaacctgc atttcaatca gatatgctgc atgcgcatga cgaaacgcgt ggatacgcag
     4441 tggctttaat ccagccttaa ctgcacttgt tttcaaggca tgatggattg ctcctttaga
     4501 cattctgaaa atcggcttat tcggatttgg attatacaac gaatccatat actcctttaa
     4561 aatagtagcc aaaaaatcag gaattgtgat ctttctttta ctctttggcg ttttaggatc
     4621 tgtaataatt tctttaccct taacgacctg caacgattta ttcacatcaa tatacttttc
     4681 gtcaatacga atatcattag gtgtcagcgc tcttaattct cccaatctca tgcccgtcca
     4741 gtaaaacgtc ataaagatcg tataaaccgt ttgattgttc attgtctgct cgatcagacg
     4801 atcaaactcc tcttttgtat aaaactctac ctcatcacca tgttcctttc caatgggacc
     4861 agctatacgt actggattat ttttcaatcc atagtatgct accgcatggt tgaaaagtgc
     4921 actaagctga ttgttcaatg ttttttgata cgtttccgaa taccctcttt caatcagctg
     4981 attctgccat tttcttacca tcgacactga aatatcatcc agtccataat tgccaaagaa
     5041 cggagtgata tgtactcgta aatatgttct cttcgtctca tacgtcgttt ttcttaatcg
     5101 aggttgtaaa tcttctagat acacatccac ataatcagaa aacttcattg aaaggttctc
     5161 gctcttttta agctcatatt cattcagcca cttctttgca tctgattttc ttttaaaacc
     5221 acgtttagtc gacttcttac aatttcccat cgcatccttt acacgaaact gcacatcata
     5281 ctttttcgtc ttaggattta acgtaatact tgccaatacg atcaactccc ttcttcatcc
     5341 ctgtttgatt tactggaata acaaatatcg ttaaaatact ccactggcag ttttccagaa
     5401 atcacaagct tatgcatact cttcaattcc tgatttcctt cacgaattat tgtgtaagcc
     5461 ttgcttttag aaacgcctaa tatttcagca atatccgcag cacctaataa agccttgcta
     5521 gacatatact cacctccttg cctgatatgt atcttcactg ctttttgtat ttgagggaag
     5581 cattgtcata aatataagct tttcttatat ttataatata ttcttatact ttgtaatttg
     5641 tcaataagta tttcatatat ttataagttt ttctgatatt tattttttga aatgatacaa
     5701 tatattcaac aggaagtgat tatatggaaa caatgggaca aagaatcaag aaaactagaa
     5761 aaatgatgaa aatgacgcag gttgaattcg caaaattatt agacgttcct caaccttcca
     5821 tttctgctta tgaaaaagat aaatatctac cttcatttga agtcatagga aaaatcatga
     5881 aagaatgtaa tgtatctttt gaatggctta acggatacaa ttctgttccg gactcaacat
     5941 taacagtcac ggatatcggc aatatgatca gccaactatt tgaaatagac agcctttcat
     6001 ttcaggtgga agctgaaatg agtcatagtt cttctaaaga caaaagtgaa tgtattcatc
     6061 ctaaagtaac ttttacattg ttgaataatg atgctcttgg tgacaatcta atttcctatt
     6121 tatatgagat gcaatccgta tgtttcgatt actcaaacca cctgatttca gaagaagaat
     6181 ctttaaacaa gaaatcacag atatatgaga aatatatgat cccaatctca ttcaagaaat
     6241 acagagaaac gtcagaaaaa gaattgatgg aaacacaggt aaaagcacta caaaaaaaat
     6301 tgaataacga aaaagaatca aacaactccg aagaaaaatt cacagttatc acaggtaaca
     6361 tcaataatct taacgacaaa aacgaataag ggttcaaaaa aaaataccat acatatcatg
     6421 tgtatggtat tttacattgc taacctaaat tattgattgt cagactctgc ctgtcggctt
     6481 tttaagattt cctttgcttc tttaacagta attgcacccg aatccgtttc actattcaag
     6541 tagatctctt catccccatt ttgtaagtaa gcttctccac ctagaaagcc atcccctaat
     6601 ggaatcactt tttcatcatc atcacatcca ggaaaatagc ctagatcctc attcgcttca
     6661 tccacttttt caacattaac cggttcttca ctcgatcttg caaaagctgg ataagctgta
     6721 acagcaacta aaccaataaa caacggaatt aataatcgaa gttttttcat tgtttcacct
     6781 ccatctttaa atattaacta catagtaata agttccttgt ataggtgaat acgtgtaata
     6841 aatcattcca cttccaccgc gacttacata ttgtgtttga ttttttccta ttactgtccc
     6901 ttgcaacgaa ccattcttcg tgcctacggc ttcagaataa tttcctactc tacctgcatc
     6961 tttgtaactt gtccatcgta aatatgttcc tgaacccgat gaagctttaa aaaaataccc
     7021 tccaaatctc caaccagttc cactaaattc actcgattga taatgactgt ttggttttag
     7081 tattaatatc tttgttgagc tatcaggcac atttgaacct cttgcaaagt tagaagcaga
     7141 accgtctagt ataatttttt tagcttcttt taccgttatc gaattaggat cgtcaaaact
     7201 attatattca actggttcaa acgttggatc gttttcatcc gttatcgttg cctctccata
     7261 tagaaatcct ccatcctcta attcaacaac cttatcattg tcattcaaat ctccaaataa
     7321 cacttctgtg ttttcatggc ttttaggctt tgtatccaac tcttctgcaa aaacagaaca
     7381 tgttgtaaat cctagtgctg ccactaaagc aactgcaagt aaagttttgt ttttcattta
     7441 tattcacctc ttcattcatt agaaaacgtt ttcctattaa aaatatacca cgctattatt
     7501 tttttacaag tgttttattt attttttgta tatgttaaaa tataagtgaa agaaggaaca
     7561 actcatggaa caattaacaa aaaaagaaag aactataatg gatattttat ggaatgccac
     7621 ttcatcttta tcagctagtg aaattaaaga cacctcacct gacttaaata tcaataccgt
     7681 tcaacaagta ttgcgtaacc tattgaaatg taagtttata gaagtagatg atattgtttt
     7741 caacaaaaag tctttaaccc gaacatataa gcctgtagtc tctcaagctc aatatatatc
     7801 tgcttttatt agcgattcga atcgttttaa actactatgt ggattagttg atgaagaaga
     7861 tgacttagat tctttaaatg aacttcaaaa gctaatcaac aaacgaaaaa aagaaattag
     7921 gtgaattata tgacatttat tttgtcaaca ttattatcta cattaatttt gacaagttgt
     7981 atgatattta tttttgattt ttgtattcat aaacaatgga acatgaatca agtattcttc
     8041 aaattatttt tgttgatcat attcataaaa ctatgttttc catttgaaac caagttatca
     8101 attcttatcc ctttagataa gattatgaat ccactaatcg atattcttca atataaacta
     8161 ttcaatcaga tttacatgta ccattttcta ctttctgttg ctgtaattgg aagcatctac
     8221 caatt
//
LOCUS       DBMDWL010000088         8223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3185_length_8223_cov_2.264959, whole genome shotgun sequence.
ACCESSION   DBMDWL010000088 DBMDWL010000000
VERSION     DBMDWL010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3185_length_8223_cov_2.264959"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="ACZPDN_07045"
     CDS             complement(<1..335)
                     /locus_tag="ACZPDN_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298414.1"
                     /translation="MLSRNNIYKELGKNINIYPFISENIKENSVNLTIGCNSWTKGSG
                     TVYWLGGDDFSLVKNSAKLKGTKHFKSGQNCVFEINYNPKSKRRKEKYIILLPHSTTI
                     IETSEVVGIG"
     gene            complement(329..487)
                     /locus_tag="ACZPDN_07050"
     CDS             complement(329..487)
                     /locus_tag="ACZPDN_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298415.1"
                     /translation="MSDKIDCRHIYLHMDDLGKVSKSSEEVKNSLEKTQQLYDSVTQN
                     YFGKEIKC"
     gene            complement(566..2815)
                     /locus_tag="ACZPDN_07055"
     CDS             complement(566..2815)
                     /locus_tag="ACZPDN_07055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1298416.1"
                     /translation="MNEILQLKGTFEHGKNSSMGSINLPKNKFVTVEHLMNLKNDLMN
                     VQKFWQNEKLQINPLVSVYYNTVVAKSNRIKGILESSSKKNNDTIVGAKFTDENKPKH
                     IITHCLESYKLGAAIENLAYVISAVQNYFGNVISHDTINDINSKKYKKLFEDKTKIKV
                     SRTRFVNTIVDAFYVEKFGVDQDVSDMDETAIVTIYETGTKTSDIMKQLDINFLDVRN
                     IDGTTLLLRPDQYRVLISKAPYLISMAVTDISKLEKDSFYDCEDIVTSIPHPGNEPTI
                     GVIDTMFDETVYFSEWVEFKNMVDGISLESSDYDHGTMVSSIIVDGPNINKELDDGCG
                     RFKVRHFGVAKSGSFSSFAVMRAIKEIVESNRDIKVWNLSLGSALEINSNFISPEAAI
                     LDKIQYENDVIFVVAGTNKTSRFNAKKIGAPADSINSLVVNSVDFNGKPADYTREGLV
                     LSFFNKPDVSYYGGTADQRIRVCTPLGEKFVYGTSFAAPWIARKLSYLIDVLGLTREL
                     AKALIIDSAAGWSNEKYSPQKIGYGVVPKHINQIIETPDDEIKFMINGITLDYNTFAY
                     NIPVPEDKEKFPFVAKATLCYFPKCSRNQGVDYTNTELDIHFGTLKKKKNGKIGINPI
                     NKNKQSEDERIILYEGQARKEYRKWDNVKLLRDELLTPTGKKRQPKPNNSNGLWGLSV
                     RTKERLNEKDGYNMRFGVIVTLKEINGVNRIKEFIQLCQFKGWIVNKLDVENQINIYH
                     VAEEEVVFD"
     gene            complement(2822..3856)
                     /locus_tag="ACZPDN_07060"
     CDS             complement(2822..3856)
                     /locus_tag="ACZPDN_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012234.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1298417.1"
                     /translation="MKKANIINLIKYHAEKNESAFRNEAYQIANEFDKNGDYQLAEYI
                     MALLSNANTFVPQMEEDDLAFVKKLEVNGDPLPLPDCIKDDIVGIVNAAGHGIGVNKF
                     LFQGAPGTGKTESVKQIARILDRELYMVDFAFLIDSKLGQTAKNIAQLFDEINGLFSP
                     EKVVVLFDEIDALALDRTDSNDLREMGRATTSVLKGLDNLNDEVLLIATTNLFNHFDK
                     ALVRRFDSVIDFNRYSHEDLVDISEIILKHYLSKFKNIGSNMRLFRKIISSVETIPYP
                     GDLKNIIRTSLAFSNPNDEFDYLRRVYRSFYKEDIKDLKHLQSQGFTVREIEILTGIS
                     KSQVQRELKE"
     gene            complement(3945..4121)
                     /locus_tag="ACZPDN_07065"
     CDS             complement(3945..4121)
                     /locus_tag="ACZPDN_07065"
                     /inference="COORDINATES: protein motif:HMM:NF021941.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298418.1"
                     /translation="MDALIDLEEEKGKLEKEIARLENKIKRCSGMLKNPGFVNKAPEA
                     KVNAEKNYRLTQKI"
     gene            complement(4385..4699)
                     /locus_tag="ACZPDN_07070"
     CDS             complement(4385..4699)
                     /locus_tag="ACZPDN_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298419.1"
                     /translation="MLEFENQNIKFKQEYVPDIRKEVMVFANAEGDTVYVGVRQDGAV
                     LVLAIGGAVIMTGAASLGTSFAILPELKKSKKQKKREFNASGSFKGLPEDDHRKIYRQ
                     KH"
     gene            complement(4827..7439)
                     /locus_tag="ACZPDN_07075"
     CDS             complement(4827..7439)
                     /locus_tag="ACZPDN_07075"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR1298420.1"
                     /translation="MTLEAKYNHLAVENDKYQNWIEKGYFTAGDLSKRPYCIVIPPPN
                     VTGKLHLGHAWDTTLQDILCRYKRLKGYDVCWVAGMDHAGIATQAKVDARLKSEGISR
                     YDIGREKFLERAWDWKEEYASTIRSQWAKLGLSLDYSRERFTLDEGLSHAVRKVFVDL
                     YKDGLIYQGERIINWDPEAKTALSNIEVEHKEIMGHMYYFKYKVVETGQELVIATTRP
                     ETMFADQAIFVHPDDERYKDIVGMHAINPANGDKLPIMADDYIDMSFGTAVMKCTPAH
                     DPNDFALAKKYNLPMPICMNPDGTMNDMCGKYAGMDRFECREALVNDFKEAGVVDHIE
                     EHLHQVGHSERTGVIVEPYLSKQWFVKMKPLAEEVLKNQEIEDQRINFVPSRFNKTFE
                     QWLENIEDWCISRQLWWGHRIPAWTNKETGEIYVGMEDPKDIENWTQDEDVLDTWFSS
                     ALWPFSTLGWPETTADLERYFPNDVLVTGYDIIFFWVARMAFQTRYCMKNRPFKDVLI
                     HGLVRDTQGRKMSKSLGNGIDPMDVIQEKGADALRFFLTTNSTPGQDLRFDETKMDAS
                     WNFINKIWNAARYVQMQIGDTKPELDMTKANSVDKWILARFNEVLDHVSTNMDKYELA
                     IVGNELYSFVWDDFCSWYIELSKATLYSEDESLVANTKAVLYTVMMGILKVLSPFMPF
                     VTEEIYLNLPHDKESLNVETWPEKVEFEYTDKEKDEMALVISAIQTVREIKVEYSMKP
                     SEDLTISLHNDNSYVALNTESTAILARMAHATVKEDLNTEDVLSRTIEKAVLTVAMDA
                     LIDLEEEKGKLEKEIARLENEIKRCSGMLKNPGFVNKAPEAKVNAEKEKLASYTEKLE
                     MTKTQLDNILKKLG"
     misc_binding    complement(7479..7671)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7746..7994
                     /locus_tag="ACZPDN_07080"
     CDS             7746..7994
                     /locus_tag="ACZPDN_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298421.1"
                     /translation="MMIKESGVNMDNQLYNQIKAVHEQYIKEQNRLEDEIDRLSREIV
                     CLDNEDEIEALNKLVRIDDIKRKMVVEFIHSLDKIVEQ"
     gene            complement(8010..>8223)
                     /locus_tag="ACZPDN_07085"
     CDS             complement(8010..>8223)
                     /locus_tag="ACZPDN_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298422.1"
                     /translation="ELCTHIPTELDMGKVYTRLIVDTLYPGEDYIEGILRIYNDEICK
                     TLDDYNNGAYYEPSYVIARAYKNGEF"
BASE COUNT         2422 a         1631 c         1130 g         3040 t
ORIGIN      
        1 ccaatcccta caacttcaga ggtttcaatt attgtagtcg aatgcggaag taagataata
       61 tatttttctt ttcttctctt tgattttgga ttataattta tctcaaaaac acaattctgg
      121 ccacttttaa agtgtttagt tccttttaac ttggcactat tcttaaccag tgaaaaatca
      181 tctcctccta accaataaac cgttccactt ccttttgtcc aactattaca tccaattgta
      241 agatttactg aattttcttt tatattttca gaaataaatg gataaatatt aatgttttta
      301 cctagttctt tatatatatt atttctactt aacattttat ctcctttcca aaataatttt
      361 gcgtcacgct atcatataat tgttgcgttt tttctaatga attcttcact tcttcacttg
      421 atttagatac tttccctaaa tcatccatat gtaaatatat atgcctgcaa tctattttat
      481 cactcatttt tcctccatct taattaaact caagcataca atctcactca aaacacagtc
      541 catgcttcag tgatatatcc tttacttagt cgaaaaccac ttcttcttca gcaacatgat
      601 agatgttaat ctgattttct acatccaatt tattaacaat ccaaccttta aattgacata
      661 attgaataaa ttctttaatg cggtttactc cattaatttc ctttagagtt acaattacac
      721 caaatctcat gttgtagcca tctttttcat ttaatcgttc tttagttctg acactcaagc
      781 cccataatcc atttgaatta tttggttttg gttgtctttt ctttcctgta ggtgtcaaca
      841 attcatcacg taacaatttg acgttatccc attttctata ctcttttcga gcttgccctt
      901 cataaagaat gatcctttca tcttcagatt gtttattctt atttataggg ttaattccaa
      961 tttttccatt ctttttcttt ttcaatgtac cgaaatgaat atctaattca gtattcgtat
     1021 agtctactcc ttggtttctt gaacattttg gaaaatagca taaagtagct ttagccacaa
     1081 atggaaattt ttctttatct tccggaacag gaatattata tgcaaatgta ttgtaatcca
     1141 gtgtgattcc attaatcata aatttaattt catcatctgg tgtttcgatt atttgattaa
     1201 tatgttttgg aactacacca tagcctatct tttgcgggct atatttttcg ttgctccatc
     1261 ctgctgctga gtcaataatc aaagccttcg ccaattctct tgttagacct aaaacatcga
     1321 tcaggtagga taattttctt gcaatccaag gagcagcaaa ggatgtccca tatacaaact
     1381 tttcacctaa tggcgtacaa actcttattc tctgatcagc agtaccacca taataactta
     1441 catctggttt gttgaaaaaa gataatacca aaccttctct agtataatcg gctggtttac
     1501 cattaaaatc aactgaatta acaactaacg agttaataga atctgcaggg gctccaatct
     1561 tttttgcgtt aaatcgtgat gttttatttg tacctgcaac aacaaatatc acatcattct
     1621 catattggat tttatctaga attgccgctt ccggagaaat gaagtttgaa ttaatttcta
     1681 atgcagaacc taaagataaa ttccagactt ttatatctct attcgattct acaatttcct
     1741 ttatggctct cattacagca aatgaactaa agctaccact tttggcaacc ccaaaatgtc
     1801 taaccttaaa tcttccacat ccatcatcca gttctttatt aatgtttggt ccatcaacaa
     1861 taatagaact taccatcgta ccatgatcat aatcagaact ttctaaacta atgccatcaa
     1921 ccatattttt aaattcaacc cattcagaaa aatagaccgt ctcatcaaac attgtgtcaa
     1981 tgactccaat tgttggctca tttccagggt gtggtattga agtaacaatg tcttcacaat
     2041 cataaaaaga atctttttca agcttagaaa tgtctgtcac tgccattgag attaaatatg
     2101 gagctttaga tattaatacc ctatattgat caggtctaag taaaagtgtt gtcccatcaa
     2161 tattccttac atctaaaaaa tttatatcta attgtttcat gatatccgaa gttttggttc
     2221 ccgtttcata aattgtaacg attgccgttt catccatatc acttacatct tgatctacgc
     2281 caaatttttc aacataaaaa gcatctacta tcgtattaac aaatcttgtt cttgatactt
     2341 tgatttttgt tttatcttcg aacaattttt tatacttttt cgaattaata tcatttatag
     2401 tatcatgact aataacatta ccaaaataat tttgaacagc agatattaca tatgcgagat
     2461 tttctatagc agcccctaat ttataacttt ctaaacaatg agtaatgata tgttttggtt
     2521 tgttctcatc tgtgaactta gctcctacaa tcgtatcgtt gtttttcttt gaagaacttt
     2581 ctaatatccc tttgattcga ttcgatttag caaccactgt gttataatac acactcacta
     2641 gtggatttat ttgtaatttt tcattttgcc aaaacttttg aacattcatt aaatcattct
     2701 tcaaattcat caagtgttca actgtcacaa atttattttt gggcaaattg atggatccca
     2761 tagaactatt tttcccatgt tcaaaagtgc cttttaattg taaaatttca ttcatataca
     2821 attactcctt taattctctt tgtacttgac tcttagatat acccgtaagt atttcaatct
     2881 ctctaacagt gaatccctga ctttgtaaat gctttagatc ctttatatcc tctttataaa
     2941 aactcctata tactcttctt aaataatcaa attcgtcatt cggattacta aatgccaaag
     3001 aagttcgtat aatgtttttc aaatctccag gatacggtat agtttcaaca gaagaaatta
     3061 tttttctgaa caatctcata ttacttccaa tatttttaaa cttagacaaa taatgtttta
     3121 atattatttc agaaatatca actaaatctt catgtgaata tctattaaaa tcaataactg
     3181 aatcaaatct tctaactaaa gccttatcaa aatgattaaa taaatttgta gtagcaatta
     3241 acagtacctc atcatttaga ttatccaatc cctttagcac tgaagtggtt gcacgaccca
     3301 tttctcttaa atcattagaa tccgtcctat ctaatgcaag tgcatctatt tcatcaaata
     3361 aaacaacaac tttttcaggt gaaaacaagc catttatttc atcaaataat tgtgctatat
     3421 tttttgcagt ttgaccaagc ttgctgtcaa tcaaaaacgc aaaatccacc atatacaatt
     3481 ctctatcaag aattcgcgct atctgtttta cagattccgt tttcccagtt cctggtgcac
     3541 cttgaaacaa gaacttatta acaccgattc catgtccagc agcatttact attcccacta
     3601 tatcgtcctt gatacaatct ggtagtggta acggatctcc attaacctct agtttcttca
     3661 caaaagccaa atcatcctct tccatctgtg gaacaaatgt attcgcgttt gataaaagtg
     3721 ccataatata ttcagctagt tgataatcac catttttatc aaattcattc gctatttgat
     3781 aagcctcatt tctaaaggca gattcatttt tttctgcatg atattttatt aaatttataa
     3841 tattcgcctt tttcatttta ataccttcca tctctcttgt tatcgttgat tttattgtaa
     3901 cacaattggg acaattgttc aatttatggg acattacatt atttttatat tttttgtgta
     3961 agacgatagt ttttttctgc gttgacctta gcttctggtg ctttattgac gaatccagga
     4021 tttttcaaca tgcctgaaca ccgtttgatt ttgttttcta ggcgtgcgat ttctttttct
     4081 agttttcctt tttcttcttc taagtcaatt aatgcatcca ttgcaagttc gattattcat
     4141 ttttttattt tctcttaatc ataatacata ataggtttcc cttcttttag caagtaattc
     4201 cccggacaat tcattttcga ttatattatg tgagcatcca tctaacgacc tccatacatt
     4261 aaatagacat cattattatc aacatagatg ttaatatatc cttgtacaaa ggatggtgct
     4321 cattatgtcc ttaacttccg ctgaatttgt ataactaaaa aagaagcatt agtaactcaa
     4381 ttcactaatg cttctgtcga tatattttgc ggtggtcgtc ttcgggcaac cccttgaaag
     4441 agcctgatgc gttgaactct cttttttttt gctttttact ttttttcaat tccggcaaaa
     4501 tggcaaaact ggtaccaagg gatgcagcac ctgtcatgat tactgcacca ccgattgcca
     4561 ataccagaac tgctccatct tgtcgtactc caacataaac tgtgtcgccc tctgcattcg
     4621 caaataccat cacttctttt cggatgtcag ggacatactc ttgcttaaat ttgatatttt
     4681 ggttctcaaa ttctaacatc ttctagcact ttctttctct tttctttctt ctttcttatt
     4741 gtgagaaaga aaggaaagtc aagagatgta gaaacaaatg ttttgtcgat taaaaaaaac
     4801 gcccagaaat ctgagcgtaa tttatactat cctaatttct taaggatgtt atctaattgt
     4861 gtttttgtca tttctagctt ttctgtgtaa gatgctagct tttctttttc tgcgttgact
     4921 ttagcttctg gtgctttatt gacgaaacca ggatttttca acatgcctga acaacgtttg
     4981 atttcgtttt ctaggcgtgc gatttctttt tcaagttttc ctttttcttc ttctaagtca
     5041 atcaatgcat ccatcgcaac tgttaataca gctttttcga ttgtacgaga aagtacatct
     5101 tctgtattta agtcttcttt gacagtcgca tgtgccatgc gtgctaaaat ggcagttgat
     5161 tctgtattta gtgctacata actattgtcg ttgtgtaatg aaatcgttaa gtcttcactt
     5221 ggtttcattg agtattcaac tttgatttca cgaaccgttt gaattgcact gattactaat
     5281 gccatttcgt ctttttcttt gtctgtgtat tcaaattcaa ctttttctgg ccaagtttca
     5341 acgtttaagc tttctttatc atgaggtaag ttcaaataga tttcttctgt cacaaatggc
     5401 atgaatggag ataatacttt taaaattccc atcatgactg tataaagaac agcttttgta
     5461 ttagcaacta agctttcgtc ttctgaatat aaagtagctt tagaaagttc aatgtaccag
     5521 ctgcagaaat catcccatac aaaactatat agttcgtttc ctacaatagc caattcgtat
     5581 ttgtccatgt ttgtagatac atggtctaat acttcattaa aacgagctaa gatccatttg
     5641 tctacactat tggcttttgt catgtctagt tctggttttg tgtccccaat ttgcatttga
     5701 acataacgag ctgcgttcca aatcttgttg ataaagttcc agcttgcatc catctttgtt
     5761 tcgtcaaaac gtaagtcttg tcctggcgtt gaatttgttg ttaagaagaa acgtaaggcg
     5821 tcagcacctt tttcttgaat cacatccatt gggtcaatac cattgcctaa tgatttagac
     5881 attttacgtc cttgtgtatc acgaacaaga ccatggatca atacgtcttt gaatggacgg
     5941 ttcttcatac agtagcgtgt ttggaatgcc atacgagcta cccagaagaa gatgatgtca
     6001 tatcctgtta ctaatacatc atttgggaaa tagcgttcta gatcggctgt tgtttctggc
     6061 cagccaagtg ttgagaatgg ccataatgca cttgagaacc aagtatccaa tacatcttca
     6121 tcttgtgtcc aattttcaat gtcttttgga tcttccattc ccacatagat ttcacccgtt
     6181 tctttgtttg tccaagcagg aattcgatgt ccccaccaca attgacgaga gatacaccaa
     6241 tcttcaatat tttctagcca ttgttcaaat gttttattga aacgagaagg tacaaagtta
     6301 attctttgat cttcaatttc ttgattcttt aatacctctt cagcaagtgg tttcatcttt
     6361 acaaaccatt gtttacttaa gtaaggttct acaattacac ctgttctttc agagtgtccg
     6421 acttggtgta agtgttcttc aatatgatcg actacaccgg cttctttaaa gtcattaact
     6481 aaagcttcac gacattcaaa acgatccata ccagcatact taccacacat gtcattcata
     6541 gttccatctg gattcataca gataggcatt ggtaagttgt attttttagc taatgcgaag
     6601 tcattagggt catgtgctgg tgtacatttc ataacggctg taccaaaact catgtcaata
     6661 tagtcatcgg ccatgattgg aagtttatct ccattggcag ggttaatcgc atgcattcca
     6721 acaatatctt tatatctttc gtcatctggg tgaacaaaga tagcttggtc ggcaaacatg
     6781 gtttcaggac gtgttgttgc aatcactaat tcttgtcctg tttctacaac tttatattta
     6841 aaatagtaca tgtgacccat gatttcttta tgttcaactt caatattact taatgcagtt
     6901 ttggcttcag gatcccagtt gatgattctt tcaccttgat agattagtcc atccttatat
     6961 aagtctacaa agactttgcg taccgcatga cttaatcctt cgtctaatgt aaagcgttca
     7021 cgactataat ctaaagataa gcctaattta gcccattggc tacgaatagt agaagcatat
     7081 tcttctttcc aatcccacgc tctttctaag aatttttcac gaccaatatc ataacgagaa
     7141 atgccttctg attttaaacg agcatctact tttgcctgtg tcgcaatacc tgcatggtcc
     7201 ataccggcta cccaacatac atcatagcct tttaatcttt tataacggca taaaatatct
     7261 tgtagtgttg tatcccatgc atgtcctaag tgtagcttcc ctgttacgtt tggtggaggg
     7321 atcacaatac aatatgggcg ttttgataga tcgcccgctg tgaaatatcc tttttcaatc
     7381 caattttggt atttatcatt ttctactgct aaatgattat attttgcttc caacgtcatc
     7441 cacaatcatc ctttctgtac atataaaaaa gcacatcccc ataggacgtg cttgacgtgg
     7501 taccacctaa ttttattctc attcacatta acgctgtgtc acgaaattta ctactacatt
     7561 tcataaattc aactcaaaga ctaccttcat ctaccctata cacaagcttt caccaaccgc
     7621 ttgctctcta aagaatacta gtaaattact ccttcttgtc atcgctttga gtctagttta
     7681 tcaaagaaaa agtaaaaact caaatcattt tgaaaaataa gttgttttat tctctcgttt
     7741 actatatgat gattaaagaa agcggtgtaa acatggataa tcaattatac aatcaaatca
     7801 aagctgttca tgaacagtat attaaagaac aaaatcgttt agaagatgaa atcgatcgtt
     7861 taagcagaga aatcgtatgc ctcgataatg aagatgaaat cgaagccttg aataaacttg
     7921 ttcgaatcga tgacatcaaa cgtaagatgg ttgtagaatt cattcatagt ttagataaga
     7981 ttgtggagca ataagtccac aatctttttt taaaactcac cgtttttata tgcgcgcgca
     8041 atcacataag aaggctcata atatgcacca ttattataat catctagcgt tttacaaatc
     8101 tcatcattat agattcttag aatcccttca atataatctt ctccaggata taatgtatcc
     8161 actataagtc ttgtatatac ttttcccata tccaattcag ttggaatatg tgtacataat
     8221 tca
//
LOCUS       DBMDWL010000089         8143 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3227_length_8143_cov_3.193982, whole genome shotgun sequence.
ACCESSION   DBMDWL010000089 DBMDWL010000000
VERSION     DBMDWL010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_8143_cov_3.193982"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            7..225
                     /locus_tag="ACZPDN_07090"
     CDS             7..225
                     /locus_tag="ACZPDN_07090"
                     /inference="COORDINATES: protein motif:HMM:NF027433.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4825 domain-containing protein"
                     /protein_id="HIR1298423.1"
                     /translation="MTYNCTDWYNNENLYIHKALVYNSVSLFKWIDNLEYITFNFSGR
                     AIQLHENIVHSTRILNKKSMIMSLYPIG"
     gene            complement(266..1912)
                     /locus_tag="ACZPDN_07095"
     CDS             complement(266..1912)
                     /locus_tag="ACZPDN_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR1298424.1"
                     /translation="MNSLKPMLLSSLKSYDKSQFVKDVTAGIIVAIIALPLSIALALA
                     SGVGPEAGIFTAIVAGFVISALGGSSVQIAGPTAAFATIVAGIVAHDGMDGLIVATIL
                     AGIFLILMGLCHFGSLIKFIPFTITTGFTSGIAVTIVIGQLKDFFGLTYPNGVKPIET
                     VEKFEAVLHHFSTINMDAVIVGGVSLAILIIAPYIFKRIPGSLLAVIAGILMVQFLPL
                     RVNTIGNLYTISNALPSLRFPSLSLNIIQNALPNALTIAVLAAIESLLSCVVADGMIN
                     GKHRSDMELVAQGAGNIASALFGGIPATGAIARTAANIKNGGRTPIAGMVHSITLVIV
                     LVVLMPYAGMIPMPTIAAILFIVAYNMCQWRTFVNLVKTAPKSDVIVLVTSFVLTVIF
                     DLVVAIEVGMVLACLLFIKRMSEETKVNGWTYVDEDTPDVDEHLQKLSLQIRVYEISG
                     PLFFGAASVIEEIVVKDFTTCLVLRMRSVPALDSTALNALKDLVQVCKSKGITIVFSH
                     VNDQPMKVMEKAGFIELVGKVNFQPSISAALDRAEEIIHK"
     gene            2037..3200
                     /locus_tag="ACZPDN_07100"
     CDS             2037..3200
                     /locus_tag="ACZPDN_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024829.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1298425.1"
                     /translation="MNKKTKIIILISLCILIAIIGIYFLFPKGSNTQNIYVQKVSTII
                     GSSNTENRYSGVVESSETVDINSDGNKSITEMYVEAGQKVRKGDKLFSYDTTEASNSI
                     AQKKLDIEAQNNEIAAQNNTIEDYKAELNKGADKVEIQARINDASFAIRQAQNTIKAT
                     QTEIDQLNKQIENSTVLSTIDGIIKEVNRDGGTDENGNQKPLVSITQTSDFRVKGSIS
                     EMGSISEGTNVIVRSRINEDQIYKGTVTKVETDPQSNSNNNVYGNDSNESASKYPFYV
                     TLDNNKGLKLGQHVYIEVDNGQSTKKKGIWLDASFIVSDDNGNSYVWVSEKGKLKKRK
                     VELGKTDEETSTTKIKSGLSEDDYIAWADDTYKEGMKTTTEYQTETDGDANAS"
     gene            3190..3876
                     /locus_tag="ACZPDN_07105"
     CDS             3190..3876
                     /locus_tag="ACZPDN_07105"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298426.1"
                     /translation="MLLNLKNIYKNYGKEPLIVPVLKDVSLEVVQGDYVAIMGPSGSG
                     KTTLMNIIGCLDRASTGTYLFEDEDISEMNDDALSDLRLNKIGFVFQNFNLLSSETAQ
                     ENVALPLIYAGIHKEERNHRADVALSKVGLQDRTTFKPSQLSGGQKQRVAIARALINH
                     PRILLADEPTGALDQASGKQVMELFKTLNDEGVTIIMITHDASVASHAKKILHIIDGE
                     IIENKQGGAA"
     gene            3873..5627
                     /locus_tag="ACZPDN_07110"
     CDS             3873..5627
                     /locus_tag="ACZPDN_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1298427.1"
                     /translation="MKPSIKKGIIITSVCAFTLVGIKFYLSLSNTVEVHSVSSLNTGY
                     WDNPSTSTGLVSNSDTQSVLYDSSKTITQVFVKEGQQVSVGDPLLSYDLTTLQSAVDT
                     SQLEVEKAQNAITLANHELKKLLNTTPVPDVVEKPEIQDPTPAPLPSVPTKNGNGLYP
                     YILSLSQAEKNFTDYKIYYTNTESEVPEKGPKEDVNAWKEERIKKTSDNTCWYWIEYT
                     YTDGSNNAYDSKDVVEYYSDKQKVPYEDISTVGTKKNPYVFKLSEDQGFVYGKLFLDN
                     KNLNSYFRFDVYTNDGEISSSWLVRCDKFATKQSMNEGDMYSVISHTKEEQKYIEAEQ
                     NQSPDNTSGYTEIELAKALRDKKQELKTLDLDLRKAKLSLSENKALLNDGIVRAKRSG
                     IVRNIKDLSNPIQDGSAFLEVATGQGTYIKGSISELMLNQVKVGDTISAYCWTSGETF
                     DAKIQSIDTVPSSNSNYNGSGNPNVSYYGFEAIAKDASNVAVGEYLELTFHSNTDTTS
                     SIWLSKAYVKQEGNKYYVLKENHGRLKKQYVTVGKIVWGDTVEIKEGLTDTDSIAFAY
                     SKNAKEGVKTKNMSEDAS"
     gene            5624..6868
                     /locus_tag="ACZPDN_07115"
     CDS             5624..6868
                     /locus_tag="ACZPDN_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1298428.1"
                     /translation="MIENIRLSFRGIFAHKMRSFLTMLGIIIGIASIIAIVSTIKGTN
                     DQIEKNLIGSGNNTVKVALYQEDLEYSFDAGIPNGVPTVSKDTLESLKNIEHVQGATL
                     YRSRQSYQAIFRNNTSLDGGYIMGVNQDYFSIAGLTIKQGRGITEDDNKKYRQVAVLD
                     ETSAKLLFGEENPIGKTIEISSTPFTIVGVCADKETFEPTMESIDDYHTYNQSTGSKV
                     YVPSESWPIFYQFDEPQNALLKVDATSNMTRVGKEAATILNTNVSSSSVQYKAQDLLK
                     QAKDIQNLSKSTNAMLIWIAGISLLVGGIGVMNIMLVTVTERTKEIGLKKAIGARKKA
                     ILFQFLTEAVVLTSIGGIVGVLTGIGLAKLISIFNGTPVSISIPAMLLSVLFSMAIGI
                     IFGLLPSYKAANLDPIEALRHE"
     gene            complement(6927..7334)
                     /locus_tag="ACZPDN_07120"
     CDS             complement(6927..7334)
                     /locus_tag="ACZPDN_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298429.1"
                     /translation="MTDLYTGQMLGSVNKMSHGLLSLEPKDKDGNPIENLEDHIIKED
                     GKELKAWDAIARYMRSFDDTDGDGIANVSKYYASTHDHKVVDDSKNIIDLIKKPNKFS
                     AMIVAIVLVIILLIVLLILLICKIVHKIKNKNR"
     gene            7915..>8143
                     /locus_tag="ACZPDN_07125"
     CDS             7915..>8143
                     /locus_tag="ACZPDN_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298430.1"
                     /translation="MKHAYNHCCNVSKDTVEITVEQKRINNALLENHNAIIKLVTKAT
                     SGYQSWERFRNGCMLILEKASILKSTRMTEAL"
BASE COUNT         2901 a         1358 c         1468 g         2416 t
ORIGIN      
        1 cttacaataa cctataattg tacagattgg tacaacaacg aaaacttata tatacataaa
       61 gctctagtgt acaattctgt atctctattc aaatggattg ataatcttga atacatcaca
      121 tttaatttta gtggaagagc tatacaatta cacgagaaca ttgtccactc aacaagaata
      181 ttgaacaaaa aatcaatgat aatgagtttg tatccgatag gatgaaatta tttgaaacaa
      241 attaaaaaaa cgcaaacgcg tttttttatt tatgaataat ttcttctgca cgatctagtg
      301 cagcactaat actaggctgg aagttaactt taccaacaag ctcaataaag ccagcctttt
      361 ccatgacttt cattggttga tcattcacat gtgagaacac aatcgtgatt ccttttgact
      421 tgcaaacctg aactaaatct tttaatgcgt ttagtgctgt actatctaat gcaggaacgc
      481 tgcgcatacg aagaactaga catgttgtaa aatctttgac tacaatttct tcaattacac
      541 tagctgcccc aaagaacaag ggtcctgaaa tctcgtaaac acgaatttga agggaaagct
      601 tttgaagatg ttcatcaaca tcgggtgtat cctcatctac atatgtccag ccattaactt
      661 ttgtttcttc actcatacgt ttaatgaata ataaacaagc aagtaccata cccacttcaa
      721 tggctacaac caaatcaaag atgacagtta gcacaaatga agtgactaaa acaataacat
      781 cactcttagg tgctgtttta actaagttca caaaggtacg ccattggcac atattgtatg
      841 cgacaataaa taaaatagct gcaatggtag gcatcggaat cattccggca tatggcatca
      901 aaacaactaa tacaatgacc aatgtgatag agtgcaccat accggcaatt ggtgttcttc
      961 caccattttt aatattggca gccgttcttg cgatagcacc tgttgcagga attcctccaa
     1021 ataaagcgga tgcgatattt ccagcacctt gggcaacaag ttccatatcc gaacggtgtt
     1081 taccgttaat catgccatcg gcaacaacac aagaaagtaa tgattcaatg gcagccaaca
     1141 cggctatggt tagtgcattc ggtaatgcat tttgaatgat atttaatgat aaacttggaa
     1201 aacgcaaaga tggtaatgca ttactgattg tataaagatt tccaattgta tttaccctta
     1261 aaggcaagaa ttgaaccatc aaaattcctg caataacggc taaaagtgag cctggaattc
     1321 ttttaaagat atatggagca ataatcaaga tagctaaaga cactccacct actatgacag
     1381 catccatatt gattgtagaa aaatgatgga gcacagcttc aaatttttca accgtctcaa
     1441 taggctttac tccatttgga taggttaatc caaagaaatc ttttaattgt ccaatcacaa
     1501 tcgtaacggc aatacccgaa gtaaaacctg tcgtaatggt aaatggaata aacttgatta
     1561 gacttccaaa atgacaaagt cccatcaaaa tcaaaaagat acccgcaaga atcgtggcaa
     1621 caataagtcc atccattcca tcatgggcta caattccagc aacaatcgtc gcaaatgctg
     1681 cggtcggtcc tgcaatttgg acactacttc cgcctagtgc tgaaatgaca aaaccagcta
     1741 cgattgcagt aaagattccg gcttccggtc ccacaccgga agcaagtgcc aaggcgattg
     1801 ataatggtaa tgcgataata gcgacaatta ttccagctgt cacatctttg acaaactgag
     1861 acttatcata acttttcagt gatgaaagta acatcggttt tagtgaattc atttgttaat
     1921 atccctctct ttttaacaac ctttaaagta taccataatt taaggatttt taaggatatt
     1981 tctataatac tatttgatat actgaatata taacgataat aaatcaggag aacactatga
     2041 acaaaaagac taaaattata attttaatct cattatgtat tttaattgcc attataggca
     2101 tctatttctt atttccaaaa ggaagcaaca cccaaaacat ctatgttcaa aaagtatcta
     2161 caatcattgg ttcttctaat acggaaaacc gatattcagg tgtagtagaa tcctcagaaa
     2221 ctgtagatat caattctgat ggaaataaat ccattactga aatgtatgtt gaagctggtc
     2281 aaaaagtaag aaaaggcgat aaattatttt cttatgatac gactgaagca tccaattcca
     2341 tcgcccaaaa aaaattggat atcgaagcac agaacaatga aatcgctgct caaaataata
     2401 caattgaaga ttataaggct gaacttaata aaggtgcaga taaagttgag attcaagctc
     2461 gaatcaacga tgctagtttc gcgattcgcc aggctcaaaa tacaatcaag gccacacaga
     2521 ctgaaattga tcagttgaac aaacaaatcg aaaattcgac cgttctttcg acaattgatg
     2581 gaattattaa agaagtcaat agagatggtg gaacggatga aaatggaaac caaaaaccct
     2641 tggtgtcgat tacacaaact tctgatttta gagtaaaagg ctctattagt gaaatgggat
     2701 ctatttctga aggaacaaat gttattgtaa gaagtcgtat aaatgaagat caaatttata
     2761 aaggtacagt taccaaagtg gaaacagacc ctcaatccaa ttctaacaat aacgtttatg
     2821 gaaatgattc aaatgaatct gcatccaaat atcctttcta tgtcacttta gacaataata
     2881 aaggcttaaa acttggacaa cacgtctata ttgaagtgga taatggacaa tccacaaaga
     2941 aaaaaggaat ttggttagat gcgagtttta ttgtttcgga tgacaatgga aattcatatg
     3001 tatgggtttc tgaaaaaggt aaattaaaga aaagaaaagt agaacttggt aaaacggatg
     3061 aagaaacatc taccacaaaa atcaagtcag gactaagcga ggatgactat atcgcatggg
     3121 ctgatgatac atataaggaa ggtatgaaga caacgacgga atatcaaacg gaaacggatg
     3181 gtgatgcgaa tgcttcttaa ccttaaaaac atttataaaa attatggaaa agaaccattg
     3241 atcgttccag ttttaaaaga tgtgagtcta gaagttgtac aaggcgacta tgttgcgatt
     3301 atgggaccaa gtggttctgg taaaacaaca cttatgaata tcattggatg tttagatcga
     3361 gcaagtacgg gtacatacct atttgaagat gaagatattt ctgaaatgaa tgatgatgca
     3421 ttaagtgatt taagattaaa taaaatcggt tttgtctttc aaaacttcaa tttattaagt
     3481 tcggaaaccg ctcaagaaaa tgtagcttta cccttaattt atgcaggaat ccataaagaa
     3541 gaaagaaatc atagggcgga tgttgcctta agtaaagttg gtttacaaga tcgtacaact
     3601 tttaaaccaa gccaattgtc cggtggacaa aaacaacgtg ttgcgattgc ccgcgccctt
     3661 atcaatcatc ctagaattct acttgccgat gagcctacag gagcacttga tcaggcgagt
     3721 ggtaaacaag ttatggaatt gtttaaaact ttaaatgatg aaggtgtgac aatcattatg
     3781 attacgcatg atgcaagtgt ggcaagtcat gccaaaaaaa tccttcatat cattgatgga
     3841 gaaatcattg aaaataaaca aggaggtgca gcatgaagcc ttctataaaa aaaggaatta
     3901 ttattacttc tgtatgtgca tttactttag ttggaattaa gttttattta agcttgtcta
     3961 atacggttga ggttcattct gtatctagct taaatacagg ctattgggat aatccatcca
     4021 cttcaacagg tcttgtatct aatagtgata cgcaatccgt actatatgat tcttctaaaa
     4081 caatcacaca agtctttgtc aaagaagggc aacaagtaag tgtaggcgat ccattgttat
     4141 cttatgattt aactacactt caaagtgcag tggatacaag tcaattggaa gtggaaaaag
     4201 cacaaaatgc gattacactt gccaatcacg aattaaaaaa attattaaat acaacgcctg
     4261 ttccagatgt tgttgaaaaa ccagaaattc aagatcctac acctgcaccg ttgcctagtg
     4321 ttcctacaaa aaatggaaat ggcttatatc catatattct ttctttatcc caagccgaaa
     4381 agaattttac ggactataag atttattata caaatacaga aagtgaagtc cctgaaaaag
     4441 gacctaaaga agatgtaaat gcatggaaag aagaaagaat taaaaaaaca tcggataata
     4501 catgttggta ttggattgaa tatacatata cagatggttc aaacaatgcg tatgattcaa
     4561 aggatgttgt agagtattat agtgacaaac aaaaggttcc ttatgaagac atttctacag
     4621 ttggaaccaa aaagaatcct tatgtgttta agttaagtga agaccaggga tttgtgtacg
     4681 gaaaactatt tttagacaac aagaatctga attcatattt tcgatttgat gtgtatacaa
     4741 atgatggtga aatcagttct tcatggcttg ttcgttgtga caagtttgcc actaaacaat
     4801 ctatgaatga aggcgatatg tattcggtga tttcacatac gaaagaagaa caaaagtata
     4861 tagaggccga acaaaatcaa agtccagata atacatcagg ttatacagaa atagaacttg
     4921 cgaaagccct tcgtgataag aagcaggaat tgaagacgtt agatttagat ttaagaaagg
     4981 caaagctttc attaagtgaa aataaagctt tattaaatga cggcatcgtt cgtgcaaagc
     5041 gcagtggtat tgtgcgtaat ataaaagatc tatcaaatcc tattcaagat ggcagtgcct
     5101 ttttagaagt ggcaaccggc caaggaactt atattaaagg aagcattagt gaattgatgt
     5161 taaaccaagt taaggttggc gataccattt cagcttattg ctggacaagt ggagaaacgt
     5221 ttgatgccaa aatccaaagt attgatacag ttccttcttc aaatagtaac tataatggtt
     5281 caggtaatcc aaatgtgagt tattatggtt ttgaagcaat cgccaaagat gcctcaaatg
     5341 ttgcggtcgg tgaatattta gaattaacct tccattcaaa tacagataca acgagttcga
     5401 tttggctttc aaaagcttat gtcaaacaag aaggaaataa atattacgtt cttaaagaaa
     5461 atcatggaag attgaagaag caatatgtaa cggttggaaa gattgtctgg ggggatacag
     5521 tcgaaattaa agaaggtctt acagacacgg attctattgc gtttgcgtat agcaaaaatg
     5581 ccaaagaagg cgttaagaca aagaatatgt cggaggatgc atcatgattg aaaatataag
     5641 attatccttt cgaggcattt ttgcccataa aatgcgatcc ttcttaacaa tgcttggaat
     5701 catcattggc attgcctcca ttattgcgat tgtttctaca attaagggaa caaatgatca
     5761 aattgaaaag aatctaattg gttctggaaa taacacggta aaagtagctt tatatcaaga
     5821 agatttggaa tattctttcg atgcaggcat tccaaatggt gtacctaccg tttcaaaaga
     5881 tacacttgaa tctttaaaaa atatcgagca tgtccaaggt gctacgcttt atcgttcaag
     5941 acaaagttat caagctatat ttcgtaataa cacttcttta gatggtggat acatcatggg
     6001 tgtgaatcaa gactacttct caattgctgg actcaccatc aaacaaggaa gaggcattac
     6061 agaagatgac aacaagaaat ataggcaagt tgccgtatta gatgaaacaa gcgcaaaact
     6121 tttatttggt gaagaaaatc cgattggtaa aacgattgag atttcttcaa caccattcac
     6181 aatcgttggt gtttgtgcag ataaagaaac ttttgagcct acaatggaaa gcattgatga
     6241 ttatcataca tataatcagt ctacaggttc taaagtctat gttccaagtg aatcttggcc
     6301 aatcttttat cagtttgatg aaccgcaaaa tgcattacta aaggtggatg ccacttcgaa
     6361 tatgactcgt gttggaaaag aagcagctac tatcttaaat acaaatgttt cttcttccag
     6421 tgttcaatat aaagcacaag atttattaaa gcaggctaaa gatattcaaa atctttctaa
     6481 atctacaaat gcgatgttga tctggatcgc cggtatttcc ttacttgtag gtggtattgg
     6541 ggttatgaac atcatgcttg taacggtaac tgaaagaacg aaagaaattg gtcttaaaaa
     6601 agctattggt gctagaaaaa aagctattct attccagttc ctgactgaag ctgtcgtact
     6661 tacttcgatt ggaggtattg taggtgtatt aacaggtatt ggtttagcta aattaatatc
     6721 aatattcaat ggtactcctg tatccattag tattccagcc atgttgttat ccgtattatt
     6781 ctctatggca atcggaatta tttttggatt gttacctagc tataaagctg caaatctaga
     6841 tccaattgaa gctttaaggc acgaataaaa gctgtaacga caaaatcgtt gcagcttttt
     6901 tattggttgt cacacctaaa atatctttat ctattcttat ttttaatctt atgaacaatt
     6961 ttacaaatca ataaaattaa aagtacaatt agtaaaataa tgactaaaac gattgccaca
     7021 atcattgcac taaatttatt tggcttttta atcaagtcaa ttatattctt actatcatcc
     7081 acaaccttat gatcatgtgt tgaagcataa tactttgaaa cattcgcaat accatctcca
     7141 tccgtatcat caaatgagcg catatatctt gcgattgcat cccaagcttt tagttctttg
     7201 ccatcttctt taatgatatg atcctcaaga ttttcaattg gatttccatc cttatcttta
     7261 ggttcaagcg acaaaagtcc atgtgacatt ttatttaccg aaccaagcat ttgacctgta
     7321 tataaatctg tcacaacatg atacaattga tcatcttgaa tctcaattct ttggccttct
     7381 tttgtaagat aaaaaacaat ctattgtgga aatgacttta cctgaagaca ataaccaaat
     7441 atttgcgagt aaataccaaa cagtattacc aagcaaagaa gaattacaac atcttttaaa
     7501 tgaagaataa aattacaaat tggacttgat ggagatatat tatgagaagc aatgatttca
     7561 atcattctta tggtaattgt agcgttgatt gtttttttct ttggtgctga aatgaacgaa
     7621 gcaatgaatg gagcaatact actttcaatg attacaggtt ttgcttgact atataaaaat
     7681 agttggggtc tagatacgct ataagattac atagggcagt gtgctaggca gctcaagatt
     7741 cgctccaaag tattttgggg cgtttttagt gcctgcagct acatattttt tgattttcaa
     7801 gataataaat atactgtatt attaaaatat aagattatta ctgtacattt atgttcttca
     7861 ttcagatata aatgtctttc aggattgtat gaatgaaact ctgatcattt tgttttgaaa
     7921 cacgcgtata atcactgttg taacgtttca aaagacacag tagaaattac tgtagaacag
     7981 aaaagaatca acaatgcatt gttggagaat cataacgcaa tcatcaaatt ggttacgaaa
     8041 gcaaccagtg gctatcaaag ctgggaacga ttcagaaacg gatgtatgct gatcttagaa
     8101 aaggcatcga ttttgaaatc gacaaggatg acagaagcat taa
//
LOCUS       DBMDWL010000090         8140 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3232_length_8140_cov_2.452295, whole genome shotgun sequence.
ACCESSION   DBMDWL010000090 DBMDWL010000000
VERSION     DBMDWL010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8140)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8140)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8140
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_8140_cov_2.452295"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="ACZPDN_07130"
     CDS             complement(<1..987)
                     /locus_tag="ACZPDN_07130"
                     /inference="COORDINATES: protein motif:HMM:NF016232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIR1298431.1"
                     /translation="MTKYIEDERTELKRELIDEVKSEIIAFLNSDGGTIYVGVDDDGS
                     IVGIKDDASKDKVDLKLGNWIQEAFFPKPSGLIQYYFNSDDVLVINISKGINKPYYLR
                     EKGPKPSGVYIRVGRSKRKANDDEILRMIMESHSYSYEDDISEEQDLTFKSFERTLEE
                     NDINLTPRLMNTMGLKAKSGKYTNLAYLLSDQSEIVVKVAEYDSEMNFKIKKSFKGSL
                     VNVLKNVEEQVERLNDLRVVIDGSSFKRIETKSYPGASIREMVLNAICHANYFIRSNI
                     KIEFFPDKAKITSPGGIFNASMDDIMNGVQTYRNPRLVHVFDKLGMIENFGTG"
     gene            complement(1248..1535)
                     /locus_tag="ACZPDN_07135"
     CDS             complement(1248..1535)
                     /locus_tag="ACZPDN_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298432.1"
                     /translation="MLQTKANDYDHSMLTMRKKLNKSEVKKYVDTFLVIIGLLIIVGT
                     VICYLFYNLNSTTMYAICGIVLVIMVLLLYIRNYKNNHYGGKFKNDFPDAH"
     gene            complement(1614..3395)
                     /locus_tag="ACZPDN_07140"
     CDS             complement(1614..3395)
                     /locus_tag="ACZPDN_07140"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298433.1"
                     /translation="MFKSLTFLWKYAWNVQKSYLVCLILYQITNTIPPLLIMFFPKVL
                     LDELMGQNRISYLLTYIIIFSIVIFLMKFLSDFLKNTAFYKRCIILEKFQVELNTKLS
                     KVDYACLEDPEFLNLKQNAEKFLYANGQGFSFVLDRAMEIISKVMIFGTVIWIIASLN
                     KAVLLVFLCLAYLNSKAQMRFKKAYAKLEMEKNPKERELSYFMNVFSDVKYGKEIRMN
                     QGQYVLMDFLKGCLHKLWKFYKNQMYIMDKSDFFMHLMDFFQRMVSYVYMVFMVSKGF
                     ISIANFTLYINAIATFTTSMDEVIDSVNDINQYSYYFEAVEKFMNLPMDIYDGKVNRN
                     MPSHFESLEFRNVGFKYGGSDKWALKDINCFMKAGDKIAVVGENGAGKTTFIKLICRL
                     YDPTEGMILLNGVDIKKYDYVQYVKFISTVFQDFQLFSMALKENVSLSRKSDENRIQD
                     IFDSLGISAFVKKYKKGLNVRVHKDFDSDGFEPSGGVAQKIAISRAIYKDTPIIILDE
                     PTAALDPKSESEIYEQFNELSKNKLAIFISHRMASTRFCDSIIVFKNGSIEEMGSHYE
                     LMKAHSVYWKLFNMQSKYYMNADSGKL"
     gene            complement(4097..4255)
                     /locus_tag="ACZPDN_07145"
     CDS             complement(4097..4255)
                     /locus_tag="ACZPDN_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298434.1"
                     /translation="MNNRFYGELILLLIACLVALPLQFIPKLKENKKVEIIFGLVVFA
                     VFGIYKAI"
     gene            complement(4484..5770)
                     /locus_tag="ACZPDN_07150"
     CDS             complement(4484..5770)
                     /locus_tag="ACZPDN_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298435.1"
                     /translation="MWTYVLFNIKSNLKRKENLILVVLFIICEIALLFILNTKSFTES
                     PYQTSLGISIASVSQKNDPKLYQEFMDEVNLIRQTIDKGEQDGKDKNWKSYCEYKVKV
                     SVLEAQWYMVTSNMPNDSYFEHADVIEELRKKYGLPDLSQYEKLIVQKSNNDINLYFC
                     SMQQARYFDYLLKHDLTPITYSYVDASSVSLQIARYILPVVLPLLVGLLLFQQRERRA
                     KTEKTILTISGVKKKYVRWNLISDVLFVLLVVFLPILVYMIVLVPFKGVSNLIYPVLY
                     YKPGFTGFHYWDTQGLDHIELVASGLTNMSVNQFTTPGMELIPLWQCTLFVAFGLVMS
                     TTFYVLLITVLSKLMKNKYLSLLVFLVVLGLCSLASPLGNMQKINTFNPMSYRDFGMN
                     LLGTSYFGYFAGIVIIGLYSVILYLVSKWCTKKVRV"
     gene            complement(5771..6844)
                     /locus_tag="ACZPDN_07155"
     CDS             complement(5771..6844)
                     /locus_tag="ACZPDN_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter permease"
                     /protein_id="HIR1298436.1"
                     /translation="MMYELKKFIFNKRNVGVFGFLVFFLVCFIGYNVYMDKHYNESQI
                     KIYQKAYRISENRMDEAESEEESEFWKKVYRNASGLIHFYYNSENKTDEFIESKLSWN
                     HLMLQANNEGYIINDFEKRDVQTLQNETKQLKYLKKNHIEMLNSPYEPNTFNIFNELF
                     NQKLYLVVMLILCILLCDIFGLEMESGFYKNLYVSRISKQKILLNKIKFSLFVAVFLI
                     IVMLLIIVLSGLFFGIGNCDYPYFYFNQMYTVKEIVAKSIILLVVESVFVVGISSLLF
                     TCSLNNGFIVAVNLILYALFFVLGNVTGYSRFFVYIPFLHIDFVNLIIHKRVVLAAII
                     SLMYFLSCSLISLQCFKKRELVR"
     gene            complement(6841..7716)
                     /locus_tag="ACZPDN_07160"
     CDS             complement(6841..7716)
                     /locus_tag="ACZPDN_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298437.1"
                     /translation="MKNVVEVKNVVKSFKDKIVLNHVSFSMKPGEIVGFVGQNGAGKS
                     TLMKCMCNLINMDEGTISICGEDVVKQREKALSYVSCMIEGPGLFENMTGKENLELMA
                     TLNHASKEKLHEMMEFTQIGSQLKTKAVSQYSMGMKQRTGLAVALMKEPKVLLLDEPM
                     NGLDATGIQGLREELIRLAKEKNVALLISSHQLGEIEKMADRIIGIHFGKIKEISMEE
                     KENVYVLETDRKIPDQSDLQIIEQTGNTYKLEVNSVMEVLSNLEKQGVQVKNITQIHK
                     TLEDVYNEFIQEASL"
BASE COUNT         2823 a         1549 c          939 g         2829 t
ORIGIN      
        1 tcctgtacca aagttttcaa tcatacctag tttatcaaac acatgtacta accttggatt
       61 acgatatgtt tgcacgccat tcataatatc atccatagat gcattaaata ttccacctgg
      121 actagtaatt tttgccttat cagggaagaa ttcgatttta atattcgaac gtataaagta
      181 attggcatgg catattgcat ttaaaaccat ttcacgaata gatgcaccgg gataagattt
      241 tgtttcaatt cgcttaaacg aacttccatc aatcacaact cgtaaatcat ttaatctttc
      301 tacctgttct tctacatttt ttaaaacatt cactaacgaa cctttaaaac tctttttgat
      361 tttgaaattc atttcagaat cgtattctgc aactttgaca acaatctcag attgatcaga
      421 taataaatat gcaaggtttg tatatttacc tgatttagct ttcaaaccca ttgtattcat
      481 taatcgaggc gttaagttta tatcattctc ttcaagtgtt cgttcaaaac tcttgaatgt
      541 aagatcctgt tcctcggaaa tgtcatcttc ataactgtag ctatgtgact ccattatcat
      601 acgtaatatc tcatcgtcat tagccttacg cttacttcta ccaacacgaa tatatacgcc
      661 tgagggtttt ggtccttttt ctcttaaata gtatggtttg tttattcctt tagaaatatt
      721 aatgaccaaa acatcatcac tattaaaata atattgaatt aatcctgaag gtttgggaaa
      781 gaatgcttct tgaatccagt ttcctaattt cagatctact ttatctttag aagcatcatc
      841 tttaatccca acaatgctac catcatcgtc cacacctaca taaatggttc caccatcact
      901 attaagaaaa gcaattattt cgcttttgac ttcatcaatc aactctcgtt ttaattctgt
      961 acgttcatct tcaatatact tagtcatttt atagtgtcac ctctaatatc aatgatatat
     1021 acaatgactc attaaatcaa tgaaaagaaa tatatttccc attgttctat cacaacagat
     1081 atttttaaat ttttatataa agcatattcc tatatcaaag tcttatatat aaacagcaat
     1141 ccaatcgccc acttaaacca aagttgcaaa agagtatcac cacaacaagt gatactcttt
     1201 tattgagaga atttaaattt atacaatcaa taattattga gttaaagcta atgtgcatca
     1261 ggaaaatcat tcttgaattt gccaccataa tgattgttct tatagtttct aatatatagt
     1321 aataaaacca ttatcactaa aacaatacca cagatggcat acattgtagt tgaatttaaa
     1381 ttatagaata aataacaaat tactgtccca acaataatta ataatccaat gattactaag
     1441 aatgtatcca catatttttt cacttcagac ttattcaact tctttctcat tgtcaacata
     1501 ctatgatcat aatcattcgc ttttgtctgt aacatttagt tttgttcttt tacatcatcc
     1561 aattgaatta tctattgctt attccaatca ttctactaat tcccaagttt ccatcatagc
     1621 ttcccactat ctgcattcat ataatatttt gactgcatat tgaataattt ccaatataca
     1681 gaatgtgcct tcattaattc ataatgacta cccatttctt caatgcttcc attcttaaaa
     1741 acaataatgc tatcacaaaa ccttgtcgat gccattctat gtgaaataaa aatagccaac
     1801 ttattttttg ataattcatt aaattgttca taaatctccg attctgattt tggatccaat
     1861 gcagccgttg gctcatccaa tatgattatt ggtgtatctt tatagatagc tcggctaatc
     1921 gcaatttttt gagcaactcc gcctgaaggt tcaaaaccat ctgaatcaaa atccttgtgt
     1981 acacgtacat tcaagccttt cttgtatttc tttacaaatg cagaaatccc caaagaatca
     2041 aatatatcct gaattcgatt ttcatcactt tttcgcgata aactcacgtt ttccttaagt
     2101 gccattgaaa ataattggaa atcctggaat acagttgaaa taaattttac atattgtacg
     2161 taatcatact ttttaatatc caccccattt aataaaatca ttccctcagt tgggtcatac
     2221 aatcgacaaa ttaatttaat aaacgtagtc ttacccgcac cgttttctcc aaccactgca
     2281 attttatctc cagccttcat aaaacaattg atatctttta aagcccattt atcgctaccg
     2341 ccatatttaa atccaacatt tctaaattct aacgattcaa aatgagaagg catattacgg
     2401 ttgacctttc catcatatat atccataggc aaattcataa atttctcaac agcttcaaaa
     2461 taataactat actgatttat atcattcaca ctatcgatca cttcatccat agaagttgta
     2521 aatgtcgcaa tcgcattgat atacagtgtg aaattagcga tggatatgaa tccttttgat
     2581 accataaaaa ccatatatac ataagaaacc attctttgaa aaaaatccat aagatgcata
     2641 aagaaatcgg atttgtccat aatatacatc tgatttttat aaaacttcca aagcttatgt
     2701 aaacaacctt ttaaaaaatc catcaataca tattgacctt gattcattcg aatttccttg
     2761 ccatacttta catccgaaaa cacattcata aaataagaca attctctttc cttcggattt
     2821 ttctccattt ccaatttggc ataggccttc ttaaatcgca tctgcgcttt actatttaga
     2881 taggcaagac ataaaaacac aagcaacact gctttattta atgaagctat gatccaaatc
     2941 actgtaccga aaatcatcac tttactaatg atttccatcg ctcgatctaa tacaaaacta
     3001 aatccttgtc cattcgcata taaaaatttt tccgcatttt gtttcaaatt taaaaactct
     3061 ggatcttcca aacacgcata atccacttta gaaagttttg tatttaactc cacctgaaac
     3121 ttttctaaaa taatacatcg cttatagaaa gccgtattct tcaaaaaatc gcttaaaaac
     3181 ttcattaaga aaatcacaat tgaaaagata atgatatatg taagtaaata tgaaattcga
     3241 ttttgtccca ttagttcatc caacaaaacc tttggaaaaa acataatcaa taaaggtgga
     3301 atcgtattcg ttatttgata caaaatcaaa caaaccaaat agcttttttg tacattccac
     3361 gcatacttcc aaagaaatgt taagctttta aacatacgat caccttcttt ataagagatt
     3421 ctagcataaa tgtaattttt ttcaatatcg tattgaatta tatttcatta tcttataaac
     3481 tgtttcatta tgttacatta tattcctaat acgtcatatg caaatagtaa attttaccta
     3541 atcatgagca ttgcattcgc taaaaagtat gctataattc aattaggcaa aagctaagaa
     3601 taactgccac gtaaaaggaa cttgaattga ccgtcaagtt ccttttatat taaaaaaagc
     3661 ggctgaccag gccactttac aatgctttac tactcagtca tctccgtcaa gccatttgca
     3721 aatatagtag gcaactacac ttgctacaac agagactaaa aagctaagaa ttgattctgc
     3781 cacgttctca cctccctcgt ggtagatgag cccaagtagt atattcattt tagcataaaa
     3841 tcaaatagca aaaaataaca attctataaa tcaactgagc atcggctgat acaataaaac
     3901 gcgagataaa ccatactaac tttattgcaa aattttatca aaaaattttc aaaaagagat
     3961 aaaagcagca ggattttatg cattttcttg aattattgca cctttattta aagcactata
     4021 tttatcattt atttttaaat tatttatcta acatctgcta atttaattca tcgcatgact
     4081 aaagtcacga gtgttcttag attgctttat aaataccaaa cacagcaaaa accacgaggc
     4141 caaaaattat ttctactttc ttgttctctt ttagtttagg aataaattga agtggaagtg
     4201 caacaagaca agcaatcaaa agaaggatga gttcaccata aaatcgatta ttcataatca
     4261 tttaaccttt caacactatt tgtttaagct aataataaca taagttgctt ttaagggtac
     4321 taaaattcat tttttttatt ttttagtacc cttaaacacg ctttttctgt gtacatcaca
     4381 tagaatatat attatttaac cgcaagttac gataaaaaca aattaaatct tcttcatctc
     4441 agctattcta acttttataa ttcaattgtt tcatcatgat atattaaacg cgtactttct
     4501 tagtacacca cttcgaaact aaatacaaaa tcacactata tagtccaata attacaattc
     4561 ctgcaaaata tccaaaataa ctagtaccta ataggttcat tccaaaatct ctgtatgaca
     4621 ttggattgaa tgtattgatc ttctgcatgt ttcctaatgg cgatgccaaa gaacaaagac
     4681 ccaagacaac taaaaataca agcaaagata agtatttgtt tttcataagt ttagataata
     4741 cagttataag taggacataa aatgttgtag acatcacaag tccaaatgct acaaatagtg
     4801 tacattgcca caatgggatc aattccattc ctggcgttgt aaactgattg acactcatat
     4861 tcgttagtcc acttgccaca agctcaatat gatccaatcc ttgtgtatcc caataatgaa
     4921 agccagtaaa tccaggctta taatataaaa ccggatagat cagattcgat acacctttaa
     4981 aaggtacaag aactatcata taaactaaaa taggcaggaa aacaaccaat agaacaaaaa
     5041 gcacatcaga aatcaaattc catctcacat attttttctt tactccagaa atagttagaa
     5101 ttgttttttc tgtctttgct cgtctttctc tttgttgaaa caatagaagt ccaaccagca
     5161 aaggtaaaac tacaggcaat atatatcgag ctatctgtag tgaaacactg gatgcgtcca
     5221 catatgaata tgttattgga gtaagatcat gttttagcaa gtagtcaaag taacgagcct
     5281 gctgcattga acaaaaatat aaattaatat cattatttga tttttggaca attaattttt
     5341 catattgcga caaatcaggt aatccatatt tcttccttaa ttcttctata acatctgcat
     5401 gttcaaaata ggaatcatta ggcatgtttg aagtcaccat ataccactgt gcttccaaca
     5461 cactcacctt tactttatat tcacaatatg acttccaatt tttgtccttt ccatcctgct
     5521 cacccttatc aatggtctga cgtatcaaat tcacttcatc cataaattct tggtaaagtt
     5581 tgggatcatt cttttgtgaa acacttgcta tactaatgcc taatgatgtt tgatatggac
     5641 tttccgtaaa tgattttgta ttcaaaataa acaacaaagc aatctcacaa atgatgaata
     5701 gtacaactag aatcagattt tcttttcttt ttaaattaga tttgatattg aaaagcacat
     5761 aagtccacat ttatctcacc aactctcttt ttttgaaaca ttgtaatgaa atcaaactac
     5821 aagacagaaa atacatcaag cttataatag cagccaggac aacccgctta tgaataatca
     5881 agttcacaaa atcaatatgt aaaaatggaa tatacacaaa gaatctagaa tagcctgtta
     5941 catttcctaa gacaaagaaa agtgcatata gaattagatt tacagctaca ataaatccat
     6001 tatttaaact gcatgtaaat aacaatgaag atatacctac cacaaataca ctttctacaa
     6061 ctaatagaat aatacttttc gcaacaattt ctttcactgt atacatctga ttaaagtaaa
     6121 agtatggata atcacaattt ccaattccaa aaaacaatcc agaaagaacg ataatcaaaa
     6181 gcatcactat tatcaaaaaa acagctacaa acaatgaaaa ctttatctta ttcaaaagta
     6241 ttttttgctt tgaaattctt gaaacatata agtttttata aaatccgctt tccatttcca
     6301 agccgaatat atcgcataat aatatgcata ggatcaacat gacaaccaaa tacaattttt
     6361 gattgaacag ttcattgaat atattaaaag tattaggttc atatggacta ttcaacattt
     6421 caatatgatt cttctttaga tatttcaact gctttgtttc attctgcaaa gtttgaacat
     6481 ctcttttctc aaaatcattg atgatatacc cctcattatt tgcctgtaac atcaaatgat
     6541 tccaactcaa ttttgattct ataaattcat ctgttttatt ttcactgttg tagtaaaagt
     6601 ggatcagacc actcgcattg cgatacacct ttttccaaaa ttcagactct tcttcacttt
     6661 ctgcttcatc cattctgttt tcagaaattc gataggcctt ttggtagatt ttgatttgag
     6721 attcattata gtgtttatcc atataaacgt tgtaacctat aaaacaaacg agaaagaata
     6781 ctagaaatcc aaatacaccg acatttcgtt tattaaagat aaacttttta agttcgtaca
     6841 tcataatgat gcctcttgaa taaactcatt gtatacatct tctaatgttt tgtgtatttg
     6901 agtaatattc ttcacttgaa ccccttgctt ttctaaattt gaaagtactt ccataacaga
     6961 atttacttct aatttatagg tgtttcctgt ttgttctatg atttgcaagt ctgattgatc
     7021 tggtattttt ctatccgttt ctaatacata tacattttct ttttcttcca tggaaatctc
     7081 tttgattttc ccaaaatgga tacctatgat tcgatctgcc attttttcaa tttcacctaa
     7141 ctgatgactt gaaattagta aagctacatt cttttccttg gccaagcgta tcaattcttc
     7201 acgtaagcct tgaatccctg tagcatccag tccattcatt ggttcatcta gtaacaatac
     7261 tttaggttct ttcattaaag ccacagctaa acctgttctt tgtttcatgc ccatggaata
     7321 ttgggatact gccttagttt tcagttgaga accaatctga gtaaattcca tcatttcatg
     7381 tagtttttct tttgaagcat gatttaaagt ggccattaat tccagatttt ctttacctgt
     7441 catattttca aataatccag gaccttcaat catgcatgat acatatgata atgctttttc
     7501 cctttgttta actacatctt ctccacaaat agaaatagtt ccttcatcca tatttatcaa
     7561 attacacata catttcatta atgtgctttt ccctgcacca ttttgtccaa caaagccaac
     7621 tatttcacca ggcttcatac taaaagaaac atgatttaat acaattttat ctttgaaact
     7681 cttaactaca tttttcacct caactacatt tttcatcttt ctcatccaac tttcttttta
     7741 gtaacagcac atcatacacg atcaagtaac cagtaactat gtatatttat agattaactg
     7801 taactatttt acatatacat gtaagcgtat atatttttac tgtaaccctt tacatattat
     7861 atatcatctg tcgtttattt attcaataac tttttttatg atttaacata tccaaaataa
     7921 tcttacatga aaataaactc agaccgctca ccgacactat tcttgatcat attaaaaaat
     7981 catcttattc aatttcaaaa agtaaatttt ttcaagagga acattcaaaa atcgaataat
     8041 aaaaaaggac tatataaaaa tagttagggt ctagatacgc tataagatta catagggtaa
     8101 tgtgctaggc agcttaaaat tcgctccaaa gtattttggg
//
LOCUS       DBMDWL010000091         8104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3254_length_8104_cov_3.354367, whole genome shotgun sequence.
ACCESSION   DBMDWL010000091 DBMDWL010000000
VERSION     DBMDWL010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_8104_cov_3.354367"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            258..1712
                     /locus_tag="ACZPDN_07165"
     CDS             258..1712
                     /locus_tag="ACZPDN_07165"
                     /EC_number="3.4.16.-"
                     /inference="COORDINATES: protein motif:HMM:NF012664.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HIR1298438.1"
                     /translation="MYKTESFKPDTFKPARFESDGKITLSGKEIPYHTICEDNVIYGP
                     DGNPVASIFTYAYFRSDVEDTSNRPVVFAYNGGPGSSCMYVHAGFLGTRRMQYDEVDR
                     ESAFGPYKVIDNPDCLIDIADIVLIDPVGTGYGVLIDESKKKDFFGIEQDAEALLTVL
                     EAWVRRYNRGLSPKYLCGESYGCTRSAVAAGIAATMGRERGYGIAFDGIVFIGNTVTV
                     GKYFGEGLPVETSVLGFPTYAGVNWYHNHPTNQSVEDFVKEAKAFADHDYVLALYKGE
                     AISEEEKEHILDKVSYYTGVSREYLIRHGLKIDDDDYRQEVLKTKGKAVSRYDGRVTR
                     PLLEPEQDEIKKALWDDATADRYDTFFYAAFTGDLLPKLNVKLDRNFIPGTDYYMHWD
                     KEEKKGTTAEELRYAMTRRPGMRAFFANGWFDLCTEFGYAWHTMDHAGLPKDRVFWKG
                     YQSGHMIYLGEDNVRELCSDIRDFILGKNPKSQF"
     gene            complement(1929..3110)
                     /locus_tag="ACZPDN_07170"
     CDS             complement(1929..3110)
                     /locus_tag="ACZPDN_07170"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1298439.1"
                     /translation="MKELSKRTKTFTDSVIRRMTRIANQYDAINLSQGFPDFDPPKEI
                     LNRLEQVTHEDFNQYAITWGAQNFRDALAKKQSKYMNLDLDSNKNIVITCGSTEAMMA
                     AMMSVCDPNDKVIVFSPFYENYGADTILCGADPIYVPLHPPVFNFDKEELENAFKQNP
                     KALILCNPSNPCGKVFSKEELEYIASLAIKYDTYVITDEVYEHIVYAPYKHTYFASLP
                     GMFERTISCSSLSKTYSITGWRLGYCIAPENIIEQIKKVHDFLTVGAAAPLQEAAVAG
                     LEFKDAYYDELQKLYTHKKDLFINGLKELNIPHTEPQGAYYVMVDISEFGYDSDLDFC
                     VDLIKNVGVAAVPGSSFFKEEENRYIRFHFAKKDETLLAALDRLKDMRTKMPYKKTVG
                     E"
     gene            3190..4323
                     /locus_tag="ACZPDN_07175"
     CDS             3190..4323
                     /locus_tag="ACZPDN_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1298440.1"
                     /translation="MEKSKVYYTDFHTRKLGEGLPTKLQLLAKQAGIANLNLDGKFVA
                     IKMHFGELGNIAYLRPNYARAIVDVVKELGGKPFLTDCNTMYPGSRKNALEHLECAWQ
                     NGFTPLTVGCPILIGDGLKGTDDIEVPVVGGEYCQTAKIGRAIMDADVFISLTHFKGH
                     ESTGFGGTIKNIGMGCGSRAGKKEQHCSGIPHVDEKLCRGCKQCLKECANDGLEYDET
                     THKMHINETNCVGCGRCLGACNFDAISFNNYNANELLNKRMAEYTKAVVDGRPNFHIS
                     LIVDVSPNCDCHAENDLPILPNIGMLASFDPLALDQACVDLCMKAKPMPGSQLDKHLH
                     DLNFCDHHDHFTNSTPESEWKSCLEHAQKIGLGNREYELVEMK"
     gene            complement(4331..4846)
                     /locus_tag="ACZPDN_07180"
     CDS             complement(4331..4846)
                     /locus_tag="ACZPDN_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1298441.1"
                     /translation="MKSTTKKLVSCALCIAIGVLLPMAFHVIPNAGSIFLPMHIPVLI
                     CGLFCGTPYGLACGMITPFLSSMTTGMPPAMILPQMMIELAVYGLVTGLCSKYIPQEN
                     EMSKLYMSLIIAMLAGRIVNGFVNTFILSTQGYTLSVFMTASFITCLPGIILQLIVVP
                     ILVRILNKTAA"
     gene            complement(4866..6050)
                     /locus_tag="ACZPDN_07185"
     CDS             complement(4866..6050)
                     /locus_tag="ACZPDN_07185"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR1298442.1"
                     /translation="MKYDFTTLLNRQGHDALALDVLPIKDVEVDPNFSRIPMWVADMN
                     YPVFENIQRHMINRINEPHFGYFEMPEDYYKAIQFWQKETHGIDVEKEAIGYENGVLG
                     ILSSALQAFTSSGEAILVQSPCYIGFIHTLNNLGRKIIDSPLIKENNWSLDYEDIEKK
                     IIKHNIHFALFCSPHNPTGRVWKKEEIQKFIDICKKHDVLVFSDEIWSDLVRKENTHI
                     PTQSVSEDAKKRTIAAYAPSKTFNLAGLVGSYHIIYNKYLRDRLNKQASLSHYNSPNI
                     LSIHALMGAYCKDGLDWVSELNEVIANNVDFAIDFIHTHFKGIEVIKPEGTYMLYLDC
                     TKYLDTHDITMDELLKKGVRYGVCWQDGRPFMNPNTIRMNLALPTSLVEEAFSRLKEF
                     VF"
     gene            6168..7433
                     /locus_tag="ACZPDN_07190"
     CDS             6168..7433
                     /locus_tag="ACZPDN_07190"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1298443.1"
                     /translation="MRTRFYNARVLTMKDFDIFLGEVWVEDNKIIYVGKVKDSSFCFD
                     REIDVQKNLIMPGFINAHTHSGMTFLRSIADDMPLQQWLNEKVFPIETKLSEEDIYYF
                     SKLAILEYLTSGITTNFDMYLNPEAIVKASLDMKYRTVLCGAVNDFCLSVEDVERCFN
                     EYNQENSLISYVLGFSHEYTNSKEKIQQVAALSHKYKAPVYTHLSETEYEVNTCKEKT
                     GLSPVEYLCDLGVYDYGGGAFHCVHMSEHDLDLFKEKHLNIVTNPASNLKLASGIAPI
                     QAMLQRKINICLGTDGPASNNCLDMFREMFLVTGLAKYKNCDASAVDAYEALKMATVN
                     GAKALNLNCGVLEAGKLADLIILDLHQPNMQPIHNIAKNIVYSGSKSNVKCTMVNGKI
                     LYEDGHFHVGFEIDALYDEVQKRVERLMR"
     gene            7430..7762
                     /locus_tag="ACZPDN_07195"
     CDS             7430..7762
                     /locus_tag="ACZPDN_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR1298444.1"
                     /translation="MNIQIFGKNKCFDTKKAQRYFKERNIKFQFINLDEKEMSKKEFE
                     SVLRQVGIDSMIDSKAKDKELLTMLNYLSDKEDKVYLNQHILKTPIVRNGKLATVGYE
                     PNIWKTWD"
     gene            complement(7759..>8104)
                     /locus_tag="ACZPDN_07200"
     CDS             complement(7759..>8104)
                     /locus_tag="ACZPDN_07200"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR1298445.1"
                     /translation="IDKVIEYDEKKIEQLSNNPNIIRNKLKIKASISNAKIFKSIQEE
                     YQTFYNYLCTFTHGKIIYETGKTTSTLSDSISNDLKKRGMKFVGSTIIYSFLQAIGVI
                     YSHEKDCFLYKD"
BASE COUNT         2673 a         1245 c         1552 g         2634 t
ORIGIN      
        1 gaaaaactta atcaaggaat tgaagaaagt caatccgttt gtagagcaga atatttttaa
       61 gagtgtagaa aatgtaagtc ttaaaaccgt gattgcctat aaggatgatc atgcaagaca
      121 tcacttttta gatacatatg atgaagaggg aaatgaatag tttctctctt tttcattaca
      181 tttacaaaaa tgtaataatt tacattgact atttttcact acgttctata atggaattat
      241 tgtggaggaa acgaattatg tataaaacag aaagctttaa accagatact tttaaaccag
      301 ctcgttttga gtcagatgga aaaattacat tgtcaggaaa agaaattcca tatcatacaa
      361 tttgtgaaga caatgtcatt tatggaccag atggaaatcc tgtcgcatcg atttttacgt
      421 atgcctattt tagaagtgat gtagaagata catccaatcg tcctgttgtc tttgcgtata
      481 atggaggtcc tggatcaagt tgtatgtatg tacatgcagg ttttttagga acacgtcgta
      541 tgcagtatga tgaagtggat cgtgaaagtg cttttggacc ttataaagtc atcgataatc
      601 cagattgttt gattgatatt gcggatattg tattgattga tcctgttgga accggatatg
      661 gtgtattgat tgatgaaagt aagaagaaag atttctttgg cattgaacaa gatgcagaag
      721 ccctattaac agttcttgaa gcttgggttc gccgctataa tcgtggcttg tcaccaaaat
      781 atttatgtgg cgaaagttat ggatgtactc gtagtgcagt cgctgctgga attgctgcca
      841 caatgggaag agaaagaggc tatggtattg cctttgatgg tattgtgttt attggaaata
      901 cagttactgt aggtaaatac tttggtgaag gcttacctgt tgaaacaagt gtattgggat
      961 ttcctacata tgcaggtgta aactggtatc acaatcatcc aaccaatcaa agtgtggaag
     1021 actttgtaaa agaagctaag gctttcgcag accatgatta tgtacttgca ctttataaag
     1081 gagaagccat ttcggaagaa gaaaaagaac atatcttgga taaggtaagc tattatacag
     1141 gtgtttctag agaatacttg attcgtcatg gcttaaaaat tgatgatgat gactatcgtc
     1201 aggaagtatt aaagacaaaa ggaaaagctg tgtctcgtta tgatggtcgt gtcactcgtc
     1261 ctttattaga gcctgaacaa gatgaaatca aaaaggcatt gtgggatgat gcgacagctg
     1321 atcgttatga tacattcttc tatgctgcat ttacaggaga tctacttcca aaattaaatg
     1381 taaaattaga ccgtaacttt attccaggaa ctgattatta tatgcattgg gataaagaag
     1441 aaaagaaagg tacaaccgca gaagaattac gttatgcaat gactcgtcga ccaggaatgc
     1501 gtgctttctt tgcgaatgga tggtttgatc tatgtactga atttggatat gcatggcaca
     1561 caatggatca tgcaggacta ccaaaagatc gtgtattctg gaaaggatac caaagtggac
     1621 atatgattta tcttggtgaa gataatgttc gtgaattatg ttcagacatt cgtgatttta
     1681 ttcttggtaa gaatcctaaa tctcaatttt aataattggc tcacttcggt gagtcttttt
     1741 tcttgtgaaa aatagtgaac ttttaatgga aaaaacagaa ttttctttat atatataata
     1801 caggatttta ttaacatttt tcattttcgt taaatgatgc tataaaacat ttttaaattt
     1861 cgttaaatat gtgcgttgaa catttttaaa attcgtgaat aatagtgata aaagacaaaa
     1921 ccgttggatt actccccaac ggttttctta taaggcattt ttgtacgcat atcttttaat
     1981 cgatctaatg ccgcaagtaa agtttcatct tttttcgcaa agtggaaacg aatatagcgg
     2041 ttttcttctt ctttaaagaa actagatcct ggaacggcag ctacaccgac gtttttaatt
     2101 aggtctacac agaagtctaa atcagaatca taaccaaact cactaatatc caccataaca
     2161 tagtaagctc cttgcggttc agtatgtgga atatttaatt cttttaagcc attgatgaat
     2221 aaatctttct tgtgtgtata tagcttttga agttcatcat aataagcatc cttaaattca
     2281 agaccggcaa cggcagcttc ttgtagagga gcagccgcac caaccgttaa aaagtcatgc
     2341 actttcttga tttgttcgat gatattttca ggggcaatac aataacccaa gcgccaccct
     2401 gtaatggaat acgttttaga tagggaacta caagaaatgg ttctttcaaa catacccggt
     2461 aaagaggcaa aatatgtatg tttgtatggg gcatatacaa tatgttcata tacttcatcc
     2521 gtaatcacat atgtatcata ttttatggca agactagcaa tatattccaa ctcttctttt
     2581 gagaagactt ttccgcaagg attgcttgga ttgcataaaa tcaatgcttt cggattttgt
     2641 ttaaacgcat tttctaattc ttctttatca aagttgaata caggtggatg taaaggaaca
     2701 taaataggat cagcaccaca aaggatagta tccgcaccat agttttcata aaatggggag
     2761 aagacaatca ctttgtcgtt tggatcacaa acactcatca tggccgccat catagcttcg
     2821 gtacttccac aagtgataac gatatttttg ttcgagtcta aatctagatt catatatttt
     2881 gattgtttct tggctaaggc atctctaaag ttttgggcac cccaagtaat cgcatactgg
     2941 ttaaagtctt catgagtaac ttgttcgagt cgattcaata tttcttttgg tggatcaaaa
     3001 tcaggaaatc cctgcgatag attgatcgca tcatattgat ttgcgattct tgtcattctt
     3061 cgaattacag aatctgtaaa tgtcttagtt ctttttgata attctttcat aatcgcactt
     3121 cctttttttt cattttttgc attgtatcat ttttttgggg attgtaaaat aaaagtgagg
     3181 tgatatttga tggaaaaatc aaaagtatac tatacggatt ttcatacacg taaattaggt
     3241 gagggtttac ctacaaaatt gcaattgttg gctaagcaag caggaattgc caatttgaat
     3301 ctagacggaa agtttgtcgc aatcaaaatg cactttggag aattaggaaa tattgcatat
     3361 ttacgcccaa attatgcacg tgccattgtg gacgtggtaa aagaattagg gggaaaacca
     3421 tttttgaccg attgtaatac aatgtatcca ggatcaagaa agaatgcctt ggaacactta
     3481 gagtgtgcct ggcaaaatgg ctttacacca cttactgtag gatgtcccat tttgatagga
     3541 gatggactaa aaggaacgga cgatatcgaa gtgcctgtcg ttggtggaga atattgtcaa
     3601 acagctaaga ttggtcgagc tataatggat gctgatgtct ttatttcttt gacacacttt
     3661 aaaggccatg aatcaactgg atttggtgga accattaaaa atataggtat gggatgtggt
     3721 tcacgtgctg gaaaaaaaga acaacactgt tcaggtattc cgcatgttga tgaaaaatta
     3781 tgtcgtggtt gtaaacaatg tcttaaagaa tgtgcgaatg atggattgga atatgatgaa
     3841 acaacacata agatgcatat taatgaaaca aattgtgttg gctgtggacg ctgtttaggt
     3901 gcatgtaact ttgatgcgat ctccttcaat aactataatg cgaatgaatt gttgaataag
     3961 cgtatggcgg aatacacaaa agctgttgtg gatggacgtc ctaacttcca tatctctttg
     4021 attgtggatg tttcaccaaa ctgtgactgt catgctgaaa atgatttacc gattttacca
     4081 aatattggaa tgttggcatc ttttgatcca cttgctttag atcaagcctg cgtagattta
     4141 tgtatgaagg caaaaccaat gccaggatca caattagata aacatctaca tgatctaaac
     4201 ttctgtgatc atcatgacca tttcacaaat tcgacaccgg aatcggaatg gaaatcttgt
     4261 ttagaacatg cacaaaagat tggtttggga aatcgagaat atgaattagt tgaaatgaaa
     4321 taaaccgtag ctaggctgcg gttttattta aaatgcgaac taaaattgga acaacgatca
     4381 attgaaggat gatgcctgga agacaagtaa taaagcttgc cgtcataaat acagatagtg
     4441 tatagccttg tgtacttagg atgaatgtat tcacaaaacc attcacgatt cttcctgcaa
     4501 gcatcgcaat aattaaactc atgtataatt ttgacatttc attttcttgt ggaatatatt
     4561 tcgaacataa accagtaact aaaccatata cggctagttc aatcatcatt tggggcaaaa
     4621 tcatagctgg aggcattcct gttgtcatac tggaaagaaa gggtgtaatc attccacatg
     4681 ccaaaccgta tggggttcca caaaataatc cacaaatcaa tacaggaatg tgcatgggta
     4741 aaaatatgct tccggcattt ggtatcacat gaaaggccat gggaagcaat acgcctatcg
     4801 caatgcatag agcacaagat actaattttt tggttgtact tttcataatc taaattcctc
     4861 attctttaaa aaacaaattc ttttaaacga gaaaatgctt cttctactaa acttgttggt
     4921 aaagctaaat tcattcgaat cgtattgggg ttcataaagg gacgcccatc ttgccaacat
     4981 acaccatagc gaacgccttt ctttaaaagt tcatccatgg tgatatcgtg tgtatctaaa
     5041 tactttgtgc aatctagata caacatatat gttccttctg gttttatgac ttcaatacct
     5101 ttgaaatggg tgtggataaa atcaatcgca aaatctacat tattcgcaat tacctcatta
     5161 agctcgctaa cccaatccaa tccatcctta caataagcac ccataagagc atgaatactt
     5221 aatatgttag gtgagttata gtgagataaa gaagcctgtt tatttaatcg atcccgaaga
     5281 tatttattat aaatgatatg gtaagaaccg actaaacctg caagattgaa tgtcttactt
     5341 ggagcataag cggcaatcgt tcttttcttg gcatcttcac ttacagattg tgtaggaata
     5401 tgcgtatttt ctttacgtac tagatccgac cagatttcat cactaaagac taaaacatca
     5461 tgctttttac agatgtctat aaatttttgg atttcttcct ttttccatac tcttcccgtt
     5521 ggattgtgtg gagaacaaaa tagtgcaaaa tgaatattgt gtttgatgat cttcttttca
     5581 atatcctcat aatcaagtga ccagttattc tcttttatta atggactatc aataattttt
     5641 cttcccaagt tatttaatgt atgaataaat ccaatataac aaggggattg tactagaatg
     5701 gcttcaccgc tagatgtaaa agcctgtaaa gcacttgata atataccaag aacaccattt
     5761 tcatatccga tagcttcttt ttctacatct atgccatgtg tttctttttg ccagaactga
     5821 atggctttat agtaatcttc tggcatttca aaataaccaa aatgaggttc attgatgcgg
     5881 ttgatcatgt gtctttgaat gttttcaaaa acaggatagt tcatatctgc aacccacata
     5941 ggaatcctag aaaaatttgg atctacctct acatctttga taggtaatac atcaagggcg
     6001 agtgcatcat ggccttgacg gtttaatagt gtagtaaaat cgtatttcat gataaaaaac
     6061 tccttattaa tttaataata taatttgaaa agtgaatatg caatttgata tttgcttttt
     6121 ttcttgttgg ttttgtggga tgattagatt agaaagaagg gatgcatatg cgtacaagat
     6181 tctataatgc acgagtactc acaatgaaag attttgatat ctttttaggt gaagtttggg
     6241 tggaagataa taagattata tatgtaggaa aagtaaaaga ttcttctttt tgttttgatc
     6301 gagaaataga tgtacaaaag aatctaatta tgccaggatt tattaatgca catacacact
     6361 ctggaatgac atttttaaga tcaatagccg acgatatgcc acttcaacaa tggcttaacg
     6421 aaaaggtatt tcctattgag acaaaacttt cggaagaaga tatttattat ttttctaagt
     6481 tagctatatt agaatatcta acgagtggaa ttaccacaaa ttttgatatg tatttaaatc
     6541 ctgaagctat tgttaaggca agcttagaca tgaaatatcg tacagtctta tgtggagctg
     6601 ttaatgattt ttgtctatct gtagaagatg tagaacgctg ctttaatgaa tataaccaag
     6661 aaaatagttt gatttcttat gtccttggct ttagtcatga atacaccaac tccaaagaaa
     6721 agattcaaca agtagctgca ttatctcata aatataaagc acctgtttat acgcatttaa
     6781 gtgaaacaga atatgaagta aatacatgca aagaaaagac gggacttagt cctgttgaat
     6841 acttatgtga tctaggtgta tatgactatg gtggtggagc ctttcattgc gtgcatatga
     6901 gtgaacatga tttggatttg tttaaagaaa aacatttaaa tatcgtcacg aatccagcat
     6961 ccaatttaaa actagcaagt ggtatagctc ctattcaagc aatgcttcaa agaaagatta
     7021 atatatgtct aggtacggat gggccagcca gtaataattg tctagatatg tttagggaaa
     7081 tgtttttggt gacaggactc gcaaaatata aaaactgtga tgcttctgca gtcgatgctt
     7141 atgaagcgtt gaaaatggca actgtaaacg gagctaaagc tttaaattta aattgtggtg
     7201 tgcttgaagc tggaaagttg gcagatttaa ttattttaga tctacatcaa ccgaatatgc
     7261 agccaattca taatatcgca aaaaatattg tatacagtgg atctaaatct aatgtaaaat
     7321 gtactatggt taatggaaaa attctatatg aagatggaca ctttcatgta ggatttgaga
     7381 ttgatgcact atatgatgaa gtacaaaaaa gagtagaaag gttaatgcga tgaatataca
     7441 gatttttggg aagaataagt gttttgatac aaagaaagca caacgctatt ttaaagaaag
     7501 aaatattaag tttcagttta ttaatttaga cgaaaaagaa atgagtaaga aagaatttga
     7561 gagcgtgctt agacaagttg gcatcgattc catgattgat tcaaaggcga aggataaaga
     7621 attattgaca atgttgaatt acttgtcgga taaagaagat aaagtgtatc tcaatcagca
     7681 catattaaaa acgccaatcg tgcgaaatgg caaattggcg acagtaggct atgaaccaaa
     7741 tatttggaaa acatgggatt agtctttgta taagaaacaa tctttttcat gagaataaat
     7801 cacaccgatg gcttgaagaa aggagtatat aatggtactt cctacaaact tcatgcctct
     7861 tttttttagg tcgtttgaga tggaatctga aagagtcgat gtagtttttc ctgtttcata
     7921 aataatcttt ccatgtgtaa aagtacataa ataattataa aaagtttgat actcttcttg
     7981 aatggattta aaaatcttcg catttgaaat acttgctttg atctttaatt tgtttcgaat
     8041 aatatttgga ttgttactta attgttcgat tttcttttcg tcatactcaa tgactttatc
     8101 aatg
//
LOCUS       DBMDWL010000092         8049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3285_length_8049_cov_2.599019, whole genome shotgun sequence.
ACCESSION   DBMDWL010000092 DBMDWL010000000
VERSION     DBMDWL010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3285_length_8049_cov_2.599019"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..3930
                     /locus_tag="ACZPDN_07205"
     CDS             55..3930
                     /locus_tag="ACZPDN_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298446.1"
                     /translation="MNSLSETIYLTADGNNAHGTVILKTGSRQKNWWGYATNYNGTGI
                     IGVYKLVTNSDDVYIKVDGAYTSVRIRTTFNPTISIPATIPDYKFTSVPYQGGFFSNA
                     IYTDTLTFTTPKYWANVPISDISKTDTQPTFNTAYVSNWWRSTGNTGWWNETHRGGVT
                     MEDDTYVKVAGNKSFLIPSGSLKIGGNGNIFFATGDNDATLKIYGQTNTKFGFETIAI
                     QTCFDNEDPQTSGYTTRYAERCNLLLQPKGGQVYIGKNLTTPGDTGYKLLVDGNQLTS
                     GSQWVNGSLFFTDITGNTADTGTSLKGVYGCIGGNDGWRIAGGAKATDAGYLEIATCD
                     NMNEPIYVRQYGSGGGWNGYASLARTFTLLDAAGRSRAPELFEAKKVHVNSLLDQSVA
                     IADISYQFQVTGTSNFTDSVDISGVTTHHNNIQIDANYSVAKPGKSSPWNGSHNNAMI
                     RMTSVNSWSPLLAQKVTNGYWVLGHYDYNNEKNKNEDFRNQWIFGYLADSNLEGTTGA
                     SNSLTTIYRLLNIGYQRMFVSAEYNAAVGSKTQPVYVQSNGNVATCSYSLSATIEAGT
                     ANRMAYYKTANQIGSSGHYVTSNQVGINASSSKNYTFYVGGDSLFDNAVTINGNVHIL
                     PDADVSLNRAGSLVIGNKAGQNLGIDGNEIMARNNSKASALYLNDEGGIVQVGQDGIT
                     IQAKASSTNLQSGGLRISSKNAGNSGNVALELYRGNNGSWQIANEGAILYFRTNWVGD
                     RKTTYAKNTLIIDNTTGSASIPYLTIGQEERNTTYGLYVVSSQSWIKNTLWTGNVYPD
                     VNNAYNIGSASNYWATGYINTLYVAGNAYANGSKRIPTTGNTSGTIGSATVPVYSDGG
                     VLKTITSYSGNAATATTASKLSRNAGSATKPIYFSGGVPVQCNNTLGVNISGNAASAT
                     YTTQLLGVQNNGSNTPYSAIEDNLIRAVWNVKGDNRWYLKAGTYNCRVNYADNAGNAD
                     TVDGYHAADLFSGGYKTTLDLSTLNNSLYYPVTGASIPTNGYHKIKVSVHLNSGTKPS
                     WATHNSGFTTNVELYTTAYGWGTTHGETIVLNDSYNFTNCGQVVGFTQNGNTSTPVLW
                     LRGGGQYFVECDYNASWTIRTSLYYTQGSSSDTYKQTVEPQSSYPGFKAGDGGAINGV
                     IRAGQVYGAVWNDYAEYRRTKEYIQPGYCVIETGKGDLIKSSERLQPGANIVSDTFGF
                     AIGETEQTKTPLAVSGRVLAYPYEDRDSYQAGDPVCSGPNGTISRMTREEVREYPDRI
                     IGTVSEIPDYEVWGTGNVKINNRIWIKVK"
     gene            3942..4352
                     /locus_tag="ACZPDN_07210"
     CDS             3942..4352
                     /locus_tag="ACZPDN_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298447.1"
                     /translation="MENFLQMLSQIFEVCVIPLLGILTAYLVQYIATKKDALIKQNDN
                     ALAAKYITMLSKTITDCVIATNQTYVDSLKAQGKFDAEAQKKAFNMTLTAVLSILNDE
                     AKEYLTAIYGDLNTYITKQIEAAVNKNKTTIEPK"
     gene            4522..4947
                     /locus_tag="ACZPDN_07215"
     CDS             4522..4947
                     /locus_tag="ACZPDN_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298448.1"
                     /translation="MYPNYNYFPQNQQPIRQQPPMQNQGILYLKGRPVSSIEEVKAIP
                     IDFDGSIFIFPDIANKQIYTKQINLDGTASINVYELKILQQPTAQPQMTDYITRDEFN
                     EQMEKIRAIFAGQVPEQASQKTTPSIPSPQPVKKEDIKF"
     gene            4959..5207
                     /locus_tag="ACZPDN_07220"
     CDS             4959..5207
                     /locus_tag="ACZPDN_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298449.1"
                     /translation="MSMNINPMQLIQMIKGGQNPQQLVMNMLEQQMQNTPMGANLLSL
                     AKQNRSADIEQIARNICSQNGKDFDKEFNAFKQMLGIK"
     gene            5234..6001
                     /locus_tag="ACZPDN_07225"
     CDS             5234..6001
                     /locus_tag="ACZPDN_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298450.1"
                     /translation="MFNNATSGYSLADIAAATGTNRNDNGFGFGGDGAWWIIILFLFC
                     FAGWGGNGNGLFGGGSTGSGITDGYILTSDFANIERKIDNVDSGLCDGFYAMNTGMLN
                     GFAGINNNITQQTIADMQNANTINAGITNLGTQLQQCCCQNRYEDAQNFAQLNYNLAD
                     QECSTRRTVSDATRDLMENQNANTRNVLGAIQEMQTQALHDKINELTAANSNLRLQAS
                     QAAQNSYLINALNPTPIPAYTVANPYSGYHNCGCGTL"
     gene            6073..6495
                     /locus_tag="ACZPDN_07230"
     CDS             6073..6495
                     /locus_tag="ACZPDN_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298451.1"
                     /translation="MELTSNVVQTVNANGNVVFITTRIPGNCSVIHTEGSGNIKMRGL
                     SNNQCRSRFKVTFTGNIAVPTGGTAGAISLTITIDGEAVRTAQMIVTPAAVEQYFNVA
                     SCVYIDVPTGCCSTAGVTNTSNQTINVQNANLIVERVA"
     gene            6500..7009
                     /locus_tag="ACZPDN_07235"
     CDS             6500..7009
                     /locus_tag="ACZPDN_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298452.1"
                     /translation="MKRLCEMKNCLISAAQSQMSNLQNCDAEELGEVIDMIKDIEQAI
                     YYCTITKAMKEKSDDEEPQRMYYKESIRKKKRNVPMDYYDRYEDEDEREYSIKIYDSR
                     EGKSPERRRMYMESKELHHDQAKKLKELEEYMQELNADILEMIEGASQEEKQLLQKKI
                     ALLATKINV"
     gene            7002..7322
                     /locus_tag="ACZPDN_07240"
     CDS             7002..7322
                     /locus_tag="ACZPDN_07240"
                     /inference="COORDINATES: protein motif:HMM:NF017885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR1298453.1"
                     /translation="MFKINGDNWRVVFVSPYHPMLKKKDGSYTLGACVDDTKKIYINN
                     KLSLEKVKQVLCHEITHAAMFSYDIQLTYDQEELLADLLATYGQEVIDITNKIFSKIK
                     NNRG"
     gene            complement(7346..7930)
                     /locus_tag="ACZPDN_07245"
     CDS             complement(7346..7930)
                     /locus_tag="ACZPDN_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298454.1"
                     /translation="MLETIGSIALKYWVELILGLIVTGGGFLIKRYLRLEKEERQREQ
                     KEYFDNVLKKIQSENQQVLKSLEKEHNKMNQNSEDKYDEINTKVDKALEAGREESKSD
                     DAVLEKKISALEKNIAALTAGVLSMQGKGFRNNCKKLLAEDHVITLDEWEELDKDHMA
                     YNGLGGNHKGDNLFSLVKKKVEAGFAEDQRPKEE"
BASE COUNT         2831 a         1237 c         1456 g         2525 t
ORIGIN      
        1 aaaactcagc ctacttctgg tgcaaattgg tgtataaaaa tttctactcc agttttgaat
       61 tcattaagtg agactattta tttaacagct gatggaaata atgctcatgg aacagttatt
      121 ttaaaaactg gttctcgaca aaaaaattgg tggggatatg ctactaatta taatggcact
      181 ggaattattg gggtttacaa acttgtaact aattcagatg atgtttatat taaagtcgat
      241 ggtgcttata cttcagtaag aattagaaca acttttaatc caacaattag tattccagcc
      301 actattccag attataaatt tacaagtgtc ccatatcaag gtggtttttt tagtaatgct
      361 atttatactg atactttaac ttttacgaca ccaaaatatt gggcaaatgt tccaatttct
      421 gatatttcaa aaacagatac acagccaact tttaatacag cttatgtttc taactggtgg
      481 cgttcaactg ggaacactgg atggtggaat gaaactcatc gtggtggcgt taccatggaa
      541 gatgatacat atgtaaaagt tgctggtaat aaaagctttt taattccaag tgggtctttg
      601 aagattggcg gaaatggaaa tatcttcttt gctactggag acaatgatgc tactttaaaa
      661 atatatggtc aaactaatac taaatttgga tttgaaacta ttgcaattca aacttgtttt
      721 gataatgaag atccgcaaac atcaggatat acaactcgat atgcagagag atgtaattta
      781 ttattacaac caaaaggtgg acaggtttat attggtaaaa atttaactac tcctggagat
      841 actgggtata aattattagt agatggtaac caattgacca gtggtagtca atgggttaat
      901 ggtagtttat ttttcacaga tattactgga aatactgctg atacaggaac cagcttaaag
      961 ggtgtttatg gttgtattgg tggtaatgat ggttggagaa tcgctggcgg agcaaaggca
     1021 actgatgcag gatatcttga aattgctact tgtgacaata tgaatgagcc tatttatgta
     1081 cgtcaatatg gtagtggagg tggctggaat ggatatgcat cccttgcaag aacattcact
     1141 ctgttagatg ctgctggacg ttctcgtgcg cctgaactat ttgaagctaa aaaggttcat
     1201 gttaatagtt tattagatca aagtgtagca atcgcagata taagctatca atttcaagtt
     1261 actggaacaa gtaattttac tgatagtgtt gatatttctg gtgtaacaac tcatcataat
     1321 aatatccaaa tagatgctaa ttatagtgtt gcgaaaccag gaaaaagttc accttggaat
     1381 ggttctcata ataatgctat gattagaatg accagtgtta atagctggtc tcctttgtta
     1441 gctcaaaaag taactaatgg ttattgggtt ttaggtcatt atgattataa taatgaaaaa
     1501 aataaaaatg aagattttag aaatcaatgg atatttggat acttagcaga tagtaatctt
     1561 gaaggtacaa ctggtgcttc aaattcatta acaactattt atagattatt aaatattggc
     1621 tatcaaagaa tgtttgtttc tgcagaatat aatgcggcag taggatctaa aactcaacct
     1681 gtttatgtcc aaagcaatgg taatgtagct acttgtagtt atagtctaag tgctactatt
     1741 gaagcaggaa ctgctaatag aatggcatat tataaaactg caaatcagat tggttcttct
     1801 ggccattatg taacttcaaa tcaagttgga attaatgcat cttcttcaaa gaattatact
     1861 ttttatgtag gtggagattc tttatttgat aatgcagtaa ctattaatgg taatgttcat
     1921 attttacccg atgcagatgt tagcttaaat agagctggtt cattagtaat tggaaataaa
     1981 gctggccaaa acttgggcat tgatggcaat gaaattatgg ctcgtaataa ttctaaagca
     2041 tctgcattat acttaaatga tgaaggcgga attgttcaag ttggtcaaga cggtattacc
     2101 attcaagcca aagcttcttc tactaattta caatcaggcg gattaagaat tagttctaaa
     2161 aatgctggca atagtggcaa tgttgcttta gaattatata gaggcaataa tggttcatgg
     2221 caaattgcaa atgaaggagc tattctttat tttagaacta attgggttgg cgatagaaaa
     2281 actacttatg ctaaaaatac tttaattata gataatacca ctggctctgc gagtattcca
     2341 tatttaacta ttggccaaga agaaagaaat actacttatg gattatacgt tgtaagtagt
     2401 caatcttgga ttaaaaatac tttatggact ggaaatgttt acccagatgt aaataatgct
     2461 tataatattg gttcagcttc taattattgg gctactggtt atattaatac tttatatgtt
     2521 gctggaaatg catacgcaaa tggaagtaaa cgtattccaa ctactggaaa tacctctgga
     2581 acaataggtt ctgcaactgt accggtatat tcagatggtg gagtcttaaa aactattact
     2641 tcttatagtg gaaatgcagc tactgcaact acagcatcaa aattaagtag aaatgcaggt
     2701 tctgcgacaa aaccgattta tttttctggt ggggttccag ttcaatgtaa taatactctt
     2761 ggagtcaata ttagtggaaa tgctgcaagt gcaacatata ctactcaatt attaggcgtt
     2821 cagaataatg gaagtaatac tccatatagt gctatagaag ataacttaat tcgagcagta
     2881 tggaacgtaa aaggcgataa tagatggtat ttaaaagctg gaacatataa ttgtagagta
     2941 aattatgctg ataatgcagg aaatgcagat accgtagatg gatatcatgc agctgattta
     3001 ttttctggcg gatataaaac aacactggat ttatctactt taaataatag tctttattat
     3061 cctgttacag gagcgtctat acctaccaat ggttatcata aaattaaagt ttctgtacat
     3121 cttaattctg gtacaaaacc atcttgggct acgcataact ctggttttac aactaatgta
     3181 gaattatata ctactgctta tggatggggc acaacacatg gtgaaactat cgttttgaat
     3241 gatagttata attttacaaa ttgtggacaa gttgtaggtt ttactcaaaa cggaaatacc
     3301 agtacgccag ttttatggtt aagaggcgga ggacaatatt ttgtagaatg tgattataat
     3361 gcatcatgga caattaggac ttcattgtat tatacacaag gaagttccag tgatacttat
     3421 aaacagactg ttgaacctca atcatcatat ccaggattta aagctggaga tggtggagct
     3481 attaatggtg ttattagagc tggtcaagtt tatggtgcag tatggaatga ctatgctgag
     3541 tatcgtcgaa ctaaagaata tattcaacca ggttattgtg tcattgaaac tggaaaagga
     3601 gatttaatta aatcttctga aagactacaa ccaggagcaa atatagtatc tgatactttt
     3661 ggatttgcaa ttggtgaaac tgagcaaact aaaactccac ttgcggtctc aggtagggta
     3721 ttagcttatc cttatgaaga tagagattca tatcaagctg gtgatccagt ttgttctgga
     3781 cctaatggaa ctatttcaag aatgactcgt gaagaagtta gagaatatcc agatcgaatt
     3841 attggtacag tatcagaaat tccagattat gaagtttggg gaactggtaa tgtaaaaata
     3901 aataatcgta tttggattaa agttaaatag gaggaataat tatggaaaat ttcttacaaa
     3961 tgttatcaca aatttttgag gtatgcgtaa ttccattatt aggaatttta acagcttatt
     4021 tagtacagta tatcgcaaca aaaaaagatg ctttaattaa gcaaaatgat aatgccttag
     4081 ctgcaaaata tattacaatg ttatcaaaga caattactga ttgtgtaatt gccacaaatc
     4141 aaacatatgt agatagctta aaagctcaag gaaaatttga tgccgaagct cagaaaaagg
     4201 catttaacat gaccttaact gcagttcttt ctatcttgaa tgacgaagcg aaagaatatt
     4261 tgacagcaat ctatggcgat ttgaatacat atattacaaa acaaattgaa gcggctgtta
     4321 ataagaataa aacaactatt gaacctaaat aaaaataagg ggtacagaat atatattctg
     4381 tacccctatt tttttgtcta attttgtagt acaaaaaaag attaaaaaag actggacttt
     4441 tcatattaag atagttagta aaaattttac atactattga aggaaagaaa gaaaaataat
     4501 tttaagaagg aggattaaaa tatgtatcct aattacaact acttcccgca gaatcaacaa
     4561 cctattagac agcaaccacc aatgcaaaac caaggtattt tgtacctaaa aggcaggcca
     4621 gtttcttcca ttgaagaggt taaagccatt ccaatagact ttgatggctc tatttttatt
     4681 tttccagata tagcgaataa acaaatttat actaaacaaa tcaacttaga tggaaccgca
     4741 tcaattaatg tatatgaatt aaaaatatta caacaaccaa ccgcacaacc acaaatgaca
     4801 gattatataa caagagatga attcaatgaa caaatggaaa aaataagagc tatatttgcg
     4861 ggccaggtac cagagcaagc gtcgcaaaag acaactcctt ccattccttc accgcaacca
     4921 gtcaaaaaag aggatattaa attttaggag gaattcttat gtcaatgaat attaatccaa
     4981 tgcaattaat tcaaatgatt aaaggaggcc agaatcctca gcaattagta atgaatatgt
     5041 tagagcaaca aatgcaaaat actccaatgg gagctaatct attgtcttta gcaaagcaga
     5101 atagaagtgc agatattgaa caaatcgctc gaaatatatg cagtcaaaat ggtaaagatt
     5161 ttgataaaga atttaatgcc tttaagcaaa tgcttgggat taaataatat attttaaaaa
     5221 ggaggacatt aatatgttca ataacgcaac aagtggctat agtttagccg acattgccgc
     5281 agctactgga accaatagaa atgataatgg atttggattc ggtggagatg gagcctggtg
     5341 gattataatt ttattccttt tctgcttcgc aggatgggga ggaaatggaa atggactctt
     5401 cggaggaggt tccacaggtt ctggaattac agatggttat attttaacct cagatttcgc
     5461 taacattgaa agaaaaattg acaatgtaga tagtggactc tgtgatggtt tttatgccat
     5521 gaacacaggt atgttgaatg gatttgctgg tattaataat aatattactc agcaaacaat
     5581 cgcagacatg caaaatgcta ataccattaa tgcaggtatt accaatttag gaactcaatt
     5641 gcaacagtgt tgctgtcaaa acagatatga ggacgctcaa aactttgctc aattaaatta
     5701 taatttagca gaccaagagt gctcaactcg tagaacagtt tctgatgcaa ccagagacct
     5761 aatggaaaat caaaacgcta ataccagaaa cgttttaggt gctatccaag agatgcaaac
     5821 acaagcttta catgataaga ttaatgaatt aactgcagca aattctaatc ttagattaca
     5881 agcaagtcaa gcagctcaaa atagctattt aattaatgct ttaaatccaa cacctattcc
     5941 tgcatataca gttgctaatc catattctgg atatcataac tgcggatgtg gtacacttta
     6001 atctaaatta gtttaatggt tagaggatct gccaaccctc taacatatta aattcctgaa
     6061 aggagactat ttatggaact cacaagcaat gtagtacaaa cagtaaatgc aaatggaaat
     6121 gttgtattta ttacaacaag aatccctggt aattgttcag ttattcatac cgagggaagc
     6181 ggaaatatca aaatgagagg cttgtctaat aatcaatgcc gctcaagatt caaagtaact
     6241 tttacaggta atattgcggt cccaactggc ggcaccgcag gagctatctc attaactatc
     6301 actatagacg gtgaggcagt tcgcactgcc cagatgattg ttacccctgc cgcagtagag
     6361 caatatttta atgtagcttc atgtgtgtac attgatgtgc ctactggatg ttgctcaaca
     6421 gctggagtaa ctaacacctc aaatcaaacg attaatgttc aaaatgctaa tctaatcgta
     6481 gaaagggtgg cttaatatta tgaaaagact ttgtgaaatg aaaaattgtt taattagtgc
     6541 ggcccaatct caaatgtcta atcttcagaa ttgtgacgct gaagagttag gcgaagttat
     6601 tgatatgatt aaggatattg aacaagctat ctattattgt acaattacta aagctatgaa
     6661 agaaaaatct gacgatgaag agcctcagag aatgtactat aaagaaagta ttcgtaagaa
     6721 aaaacgtaat gtacctatgg actactatga tagatacgaa gatgaagatg aaagagaata
     6781 ttcaattaaa atatacgata gccgtgaagg taaaagccct gagcgcagac gtatgtatat
     6841 ggaatccaaa gaacttcatc atgaccaagc taaaaaatta aaagaacttg aagaatatat
     6901 gcaagaactt aatgcagata ttctcgaaat gattgagggc gcaagccaag aggaaaaaca
     6961 gttacttcaa aagaaaattg ctttattagc aactaaaata aatgtttaaa attaatgggg
     7021 ataattggcg agtcgtcttt gtatccccat atcacccaat gcttaaaaag aaagatggct
     7081 cttatactct tggagcttgc gttgatgata caaagaaaat ttatattaat aataaacttt
     7141 ctctcgaaaa agttaaacaa gttttatgcc atgaaataac acacgccgca atgtttagct
     7201 atgatattca attaacttat gaccaagagg agctgttagc cgatttactc gctacttatg
     7261 gacaagaggt aattgatata accaataaga ttttttctaa gataaaaaat aataggggat
     7321 agaattaaat tctatcccct cttttttatt cttcttttgg acgttggtct tctgcaaagc
     7381 cggcttcgac tttctttttt actaatgaaa ataaattgtc gcctttatga ttaccaccta
     7441 acccattata agccatatgg tctttatcta attcttccca ttcatcaaga gtaataacat
     7501 gatcttcagc taaaagtttt ttacaattat ttctaaaccc ttttccttgc attgataaaa
     7561 cacctgcggt caaagctgca atattttttt ccaatgctga aatttttttt tcaagaaccg
     7621 catcatcaga tttagattct tcccgacccg cttctaaagc tttatcaacc ttggtattaa
     7681 tttcatcata cttatcttca gaattttgat tcatcttatt atgttctttt tctaaagatt
     7741 ttaatacctg ttgattttca ctttgaatct ttttaagcac attatcaaaa tattcttttt
     7801 gctctctttg acgttcttct ttttcaagtc ttaaataacg ttttattaaa aaaccgccgc
     7861 cagttacaat caaacctaaa ataagttcaa cccaatactt taaagcaatt gatccaattg
     7921 tttccagcat agttaaaagc cctccttatt tttaaacttt ctctctacag attttaaaaa
     7981 acaagaaggg ttaattctat tagaatggcc aactcattac acattcattt gcatatacac
     8041 cattatcag
//
LOCUS       DBMDWL010000093         8017 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3306_length_8017_cov_3.971450, whole genome shotgun sequence.
ACCESSION   DBMDWL010000093 DBMDWL010000000
VERSION     DBMDWL010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3306_length_8017_cov_3.971450"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="ACZPDN_07250"
     CDS             <1..1066
                     /locus_tag="ACZPDN_07250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1298455.1"
                     /translation="DLKLKAYKADMKEDVKPESFVESIKDDSKVSLSFKEKPTKDKEQ
                     TITIIAKDEHGNKAMKETTLMLVKDIEKPVIHTDTISVYVGSKPNYKSYIEVTDNLDS
                     KPKIKIDSSKVKAKKTGTYKLLVTATDRSGNKAKAKINVIVEKPSKVVYMTFDDGPSE
                     NTDKILKILKKYDAKATFFVTGNNQKYNSSIKKAADQGNTIALHTYTHDYANVYSSTT
                     AYFEDLQKVSDMVKQITGKAPKYIRFPGGSSNTISAQYSQGIMSALDNMVHEKGYEYF
                     DWNCSSGDAASNTVPTQDIVRNATSCDYEQIMILFHDSSPKTTTVEALPEIIKSYKER
                     GYVFKGICDDTPIFHHGVNN"
     gene            complement(1118..1609)
                     /locus_tag="ACZPDN_07255"
     CDS             complement(1118..1609)
                     /locus_tag="ACZPDN_07255"
                     /inference="COORDINATES: protein motif:HMM:NF021619.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HIR1298456.1"
                     /translation="MLPKEAIQKVMDAYYHITGLRCYFVQDETEISSAKEKNFFCKCL
                     KTSSSALRQCDECTFENYTGALKSNEPQKYACHAGLVKWSVPVSLAGVKGVIVSEGVI
                     TKQQGLEAQDWVNHLAETYNVSRPILLHNYTKVAVMNENQVEESIKLMQDLLKYYKAV
                     IEG"
     gene            complement(1736..2650)
                     /gene="trxB"
                     /locus_tag="ACZPDN_07260"
     CDS             complement(1736..2650)
                     /gene="trxB"
                     /locus_tag="ACZPDN_07260"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR1298457.1"
                     /translation="MERQYDLVIIGAGPAGLSAALYGSRAGLKTLILENGAPGGKLIK
                     TNKISNYPGVKEIEGTQLAMDMFDQATSFQAEYAYGEVNKIDENKDVYLSDGSVVNGK
                     AIILATGTKERLLNIPGEKENLGHGVSFCAVCDGAFFRDKDVVVIGGGNSALEESIYL
                     AGLARSVTIVIRRDVFRAEEHIQSQIEENEKIHVIKKHIPVQILSSEGKVSGIELENV
                     DTHETQIVECSGIFPYVGQDPNTDCVQDLGVLNERGYVLVDANCQTKIPGIYAAGDVI
                     DKELRQVVTACNDGAIAAQHAFHTIKGI"
     gene            complement(2626..4083)
                     /gene="lgt"
                     /locus_tag="ACZPDN_07265"
     CDS             complement(2626..4083)
                     /gene="lgt"
                     /locus_tag="ACZPDN_07265"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1298458.1"
                     /translation="MHLFPSTKIFVSFGSFEIAWYAVLILTGALCAYLLCQRTMKKWG
                     YAPEVLDDYVVPMLFIGILGARAWYVIFEWQYYSQHMNEIVAIWNGGLAIHGGLIAGF
                     IFSLFFFKRRKISFLRMFDLIMPTVLLAQAFGRWGNFMNQEAYGGIVPESFFAHYPAF
                     IKNQMFIDGAYRMPTFLFESVCNLLGFLFITFIFRKYWYKRRGDCGFMYMVWYGITRF
                     VIEGMRTDSLMVLGLRTAQLVSLALMGVGCLGLMGVFHKTFHWKKKPVVLFDLDGTLI
                     DSQQLVFETFRRVFKELKPDYELSNEELYTFFGPTLEVTFSKYFPEDQVQSIIDRYQI
                     INKSLHKELLKEIPHAKEMLEGLKKENIQCAVVSNKRIEVVKRGLKQSGLDVYFDVVL
                     GKENLPEPKPSASGLIEACNLLHTSHDDCVYVGDNVADIVAAKNMAAYSVGFSVDEKQ
                     REALKEAKPCKVIDDLMQLISLCKEDHIWSDNTIW"
     gene            complement(4061..5023)
                     /gene="hprK"
                     /locus_tag="ACZPDN_07270"
     CDS             complement(4061..5023)
                     /gene="hprK"
                     /locus_tag="ACZPDN_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR1298459.1"
                     /translation="MEKVIVRDLAKKFMWTIVTGDSNSLNRPLTLADTNRPGLELAGY
                     FPDTQTKRLVVLGDKEIGYIEEQMDEISQRRSFEFLTGDNTPCIVIAHGHECPPVLKE
                     IAKRKDFPVFKTEHQTSHAIVSITNYLDECLAESVVIHGELVRVFGVGVLITGKSGMG
                     KSEIALELIKRGHQLVGDDRIDCYKIHDDLVGRTTPMLEGFMELRGVGIINVSRMYGV
                     GAVAHETQIEFQIDLEPFNDSYEYDRVGIEEKEYNEILGIKILKMTIPVSQGRPMSSV
                     IETAVTNFLLLKDGLDSAKEFEERVLSQISENKKEEFDASVSEH"
     gene            complement(5001..7850)
                     /gene="uvrA"
                     /locus_tag="ACZPDN_07275"
     CDS             complement(5001..7850)
                     /gene="uvrA"
                     /locus_tag="ACZPDN_07275"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1298460.1"
                     /translation="MDQKYITIQGARENNLKNISLKIPKDKLIIMTGVSGSGKTSLAF
                     DTIYAEGQRRYMESLSAYARQFLGNSEKPDVDQIDGLSPAIAIDQKTTSNNPRSTVGT
                     VTEIYDYLRLLYARIGVPYCPDHHIPITGNTIKEMIDKIMELPDKTRCTILSPVIQGK
                     KGTHKDLIEKLMKEGYIRVRIDGEIKYLEELEPLDKNKKHTIDVVVDRIVKSDDRSRF
                     HDSLETALKLSDGLAILLTNESETLFSSNYACKICGFSVPKLEPKLFSFNAPFGACPE
                     CKGLGITQKVDLSLLIPDDTKSIAQGGIHYYKNIVNTENLEWQRIHALIKYYEIDMDT
                     PIKELPKKKLNYLLYGSDVPIAYQLNSRSGTVSTKFEYIEGVCPMIERRYMETTSTMS
                     REWYGSFLADAQCPKCHGARLNKKALSVLVGGKNIYEWTQMSIQEAIQFMNELELTPT
                     QAKIAELVIKEIKNRLSFLNHVGLSYLTLDRLASTLSGGEAQRIRLATQIGSRLTGVM
                     YVLDEPSIGLHQRDNDRLIGALKDMRDLGNTLIVVEHDEDTMRASDYIVDVGPGAGVH
                     GGQIVAAGTPEEIMQNENSITGAYLSGRKRIEVPMTRRKGNGKKLKVVRASQNNLKNV
                     NVTIKLGTFTVVTGVSGSGKSSLVTEVLTHGLQHALGRLRIKPGACKEIQGIENIDKI
                     VEIGQDPIGRTPRSNPATYTGVFDDIRDLFAQTKEAKMLGYDKGRFSFNVKGGRCEAC
                     QGDGILRISMHFLPDVYVPCDQCGGKRYNEETLQVHYKGKTIADVLDMTVEEALEFFS
                     NVSKIKHKLQTLNDVGLSYIKLGQSATTLSGGEAQRVKLASELQKKATGKTLFVLDEP
                     TTGLHSDDVKKLIEVLQRLVEHGDTVLVIEHNLDVIKCADQIIDMGPEGGQGGGTVIC
                     TGTPEKIAECKESYTGQYLKPLLEKGGDHGKSNCS"
BASE COUNT         2533 a         1656 c         1162 g         2666 t
ORIGIN      
        1 tgatttaaaa cttaaagcat ataaggcaga tatgaaagag gatgttaagc ctgaatcctt
       61 tgtcgaaagt attaaggacg attctaaagt atcactatcc tttaaggaaa aacctacaaa
      121 ggataaagaa caaacgataa ccatcatcgc aaaagatgaa catggaaata aagccatgaa
      181 agagacaact ctaatgttgg tcaaagatat tgaaaaacca gttattcata cagatactat
      241 ttctgtttat gtgggttcaa agccaaacta caaatcctat attgaggtaa cagacaattt
      301 ggattctaag ccaaaaatca agattgattc ttcaaaagta aaggccaaga aaacaggtac
      361 ttataaattg ttagtaactg ctacagatcg ttcgggtaat aaagctaagg ctaaaatcaa
      421 tgtgattgtt gagaaaccta gcaaggttgt atatatgaca tttgatgatg gtcctagtga
      481 aaatacagat aaaatattga aaatcttaaa gaaatatgat gcgaaagcta ctttctttgt
      541 gacgggaaac aatcaaaaat ataacagttc tatcaaaaag gctgcagatc aaggcaacac
      601 aattgcactt catacttata cacatgatta tgcgaatgtt tattcttcta ccactgctta
      661 ttttgaagat ttacaaaaag tgagtgacat ggtcaaacaa atcacaggta aagcaccaaa
      721 atatattcgt ttccctggtg gttcaagcaa tacaatttca gcacaatatt cgcaaggaat
      781 catgtctgca ctcgacaata tggtgcatga aaaaggatat gaatattttg attggaactg
      841 ctcaagtgga gatgctgcat ccaataccgt gccaacacaa gatattgtgc gcaatgcgac
      901 aagttgcgat tacgaacaaa tcatgatttt attccacgat tcaagtccaa aaacaacaac
      961 ggttgaagca ttaccagaaa tcattaaaag ctataaagaa agaggatatg tctttaaagg
     1021 aatctgcgat gatacgccaa tcttccatca tggagtcaat aactaagtca gcaaatggct
     1081 ggcttttttt tgtacctgca gaaaaaaatc gaaggaatta accttcgatt actgccttgt
     1141 agtactttaa taaatcttgc atcaatttga tgctttcttc tacctgattt tcattcatca
     1201 ctgcaacttt tgtatagtta tgtaataaaa taggtctaga aacattatat gtttcagcta
     1261 aatgattgac ccaatcttgt gcttctaagc cttgctgttt tgtgataaca ccttctgata
     1321 caattacacc ttttacacct gcaagagata cgggtacact ccatttaact agtcctgcat
     1381 gacatgcata cttttgtggt tcgttcgatt ttaaagctcc tgtataattt tcaaaagtac
     1441 attcatcaca ttgacgtaat gcagatgaac ttgtttttaa acatttacaa aagaagtttt
     1501 tctcctttgc gctactaatc tctgtttcgt cttgcacaaa atagcatctt aatccggtaa
     1561 tatggtaata cgcatccatt accttttgga ttgcttcttt aggcaacata aataacacct
     1621 caaatccttt tcttatctta attaaatcac tttcaatgat tgatttcaag gcttttgtaa
     1681 gctgtttcag ctatgctttt cgtatattaa aaccagatgt tatcatctgg tcatattaaa
     1741 ttccttttat tgtatgaaat gcatgttgcg cggcaatggc accatcatta catgcggtaa
     1801 caacctgcct taattcttta tcaatgacat ctccagccgc ataaattcct ggtatctttg
     1861 tttgacaatt cgcatctacc aaaacatatc cacgctcatt taatacacct aaatcttgta
     1921 cacaatccgt atttggatct tgtccaacat aagggaaaat gcctgaacat tcaacaatct
     1981 gtgtttcatg tgtatccaca ttttctaatt caatacccga tacttttcct tctgaactta
     2041 aaatttgaac aggaatatgc ttcttgatga catgaatctt ctcattttct tcaatctgac
     2101 tttgaatatg ttcttccgct ctaaatacat ctcttcgaat cacaatcgtt acacttcgtg
     2161 caagccctgc aagataaatc gattcttcta atgccgagtt tcctccgcca atcacgacta
     2221 catctttatc tctaaaaaat gccccgtcac aaacggcaca gaaagaaaca ccatgtccaa
     2281 gattttcttt ttctccaggt atatttaaca atcgttcttt tgtacctgtg gcaagtatta
     2341 tggctttacc attcacaaca cttccatcgc ttaaatatac atctttattt tcatcaatct
     2401 tatttacttc gccatacgca tattcagctt gaaaagaagt agcttgatca aacatatcca
     2461 tcgcaagctg cgttccttct atttctttaa cacctggata attactgatt ttattggttt
     2521 taatcaactt tcctcccggc gctccatttt ctaaaattaa tgtttttagt ccggcccttg
     2581 agccataaag tgctgcactt aagcctgctg gcccagcacc tattatcacc aaatcgtatt
     2641 gtcgctccat atgtgatcct ccttacataa tgatattaac tgcatcaaat catcaataac
     2701 tttacatggc tttgcttctt ttaatgcttc acgttgtttt tcatcgacac taaatcctac
     2761 agaataggca gccatattct ttgcggctac aatatccgca acattgtcac cgacataaac
     2821 acaatcatca tgactagtat gtaataagtt acacgcttca atcaagccag atgcacttgg
     2881 tttaggttct ggtaaatttt cttttcccaa cacaacatca aaatacacat ctaatcccga
     2941 ttgttttaat cctcttttta cgacttcaat acgcttattt gaaacaactg cacattgtat
     3001 attttctttt ttcaatcctt caagcatctc tttagcatga gggatttctt ttaataattc
     3061 tttatgaagc gatttattga tgatttgata acgatcaatg atactttgaa cttgatcttc
     3121 tggaaaatac tttgaaaaag taacttcaag tgtgggtcca aaaaatgtat acaattcttc
     3181 atttgacaat tcataatctg gtttaagttc tttaaatact cttctaaatg tttcaaaaac
     3241 caattgttgt gaatcaatca atgtcccatc taaatcaaac aacacaacag gctttttctt
     3301 ccaatgaaat gttttatgaa atacccccat taagccaaga caaccaacac ccattaaagc
     3361 taaagataca agttgagctg tacgaaggcc caataccatt aaagaatctg ttcgcattcc
     3421 ttcaatcaca aaacgtgtaa tgccatacca aaccatgtac ataaatccgc agtcaccgcg
     3481 tcttttatac caatacttac ggaaaataaa tgtaataaac aagaatccta atagattaca
     3541 tacactttca aataagaaag taggcattcg ataggctcca tcaataaaca tttgattttt
     3601 aataaatgct ggataatgcg caaagaagct ctcaggtaca atgccaccat aggcttcttg
     3661 attcataaag tttccccaac gaccaaatgc ttgtgcaagt aaaacggttg gcatgattaa
     3721 atcaaacatt cttaaaaatg aaatttttcg tcttttgaaa aagaacaaac taaatatgaa
     3781 acctgcaatc aatcctccat gaatggccaa accgccgttc caaatggcaa cgatttcatt
     3841 catatgttga gaataatatt gccattcaaa aataacatac catgcccttg cacctagaat
     3901 ccctataaat aacataggaa cgacataatc atctagaact tctggtgcgt acccccattt
     3961 tttcatggtt ctttggcata gtaaataggc gcataatgcg cctgttaaaa ttaaaacagc
     4021 ataccatgca atttcaaaag atccaaaaga aacaaaaatt ttagtgctcg gaaacagatg
     4081 catcgaattc ctccttctta ttctcggata tttgcgatag tactcgctct tcaaattctt
     4141 tagctgaatc taagccatct ttcaacaata agaagtttgt cacagccgtt tcaataacag
     4201 atgacattgg tcgtccttgt gaaactggaa ttgtcatctt taaaattttg attcctagaa
     4261 tttcattata ttccttttct tcaattccaa cacgatcata ttcataagaa tcattaaatg
     4321 gttctaaatc gatttgaaat tcaatttgag tttcatgggc aacagctcct actccataca
     4381 tgcgagagac attgataatt cccacacctc taagttccat aaaaccttca agcattggtg
     4441 ttgttctacc aactagatca tcatgaattt tgtaacaatc aatacggtca tctccaacta
     4501 attggtgtcc acgcttaatc agttctagcg caatttcact ttttcccatt ccacttttac
     4561 cagtaatcaa tacaccaaca ccaaataccc gtactaattc tccatgaata actacagatt
     4621 ctgctagaca ttcatctaag tagttcgtaa tacttacgat tgcgtgagaa gtttgatgtt
     4681 ctgttttaaa tactggaaaa tctttacgct ttgcgatctc ttttaaaaca ggtggacact
     4741 catggccatg tgcaatcaca atacatggtg tattatctcc tgttaaaaat tcaaaggaac
     4801 gacgttgtga aatttcatcc atttgttctt caatataacc aatttcttta tctcctaata
     4861 ctacaagtct ttttgtttga gtatccggaa aatatccagc caactctaat cccggacgat
     4921 ttgtatcagc caaagttaat ggacgattta atgaatttga atctccagtc acaattgtcc
     4981 acatgaattt ttttgctaaa tcacgaacaa ttactttttc catggtctcc tcctttttct
     5041 aataatggtt ttaaatattg acctgtataa ctttctttgc attcggcgat cttttcaggt
     5101 gttcccgtac aaataacagt acctccccct tgacctcctt caggccccat atctataatt
     5161 tgatctgcac atttgattac gtccaagtta tgttcaataa ctaatactgt atctccatgt
     5221 tcgactaaac gctgtaaaac ttcaatcaat ttttttacat catcactatg caatcctgtt
     5281 gtaggttcat caagcacaaa taacgtttta cctgtagcct tcttttgaag ctcactcgct
     5341 agttttacac gttgtgcctc gccccctgat aaagtcgttg cactctgacc taattttata
     5401 taagaaagtc ctacatcatt tagagtttgt aatttatgtt tgatttttga aacattgcta
     5461 aagaattcca atgcctcttc aaccgtcata tccaatacat ccgcaattgt tttaccttta
     5521 taatgaactt gtaatgtttc ttcattatat cgttttccgc cacattgatc acaaggtaca
     5581 taaacatctg gtaaaaaatg catggaaata cgtagaattc catcgccttg acatgcttca
     5641 catcgtccac ctttcacatt gaatgaaaaa cgacccttgt catatccaag cattttcgcc
     5701 tcttttgtct gcgcaaacaa atcacgaata tcatcaaaaa caccggtata cgtagctgga
     5761 tttgatcgtg gtgttctacc aattggatct tgtccaattt ctacaatttt atcaatattt
     5821 tcaatacctt gaatttcttt acatgcaccc ggtttgattc ttaaacgacc tagggcatgt
     5881 tgtaacccat gtgttaaaac ttctgtaact aatgaagatt ttccacttcc agaaacccct
     5941 gtgacaaccg taaatgttcc taacttaatc gttacattta cattttttaa gttgttctgg
     6001 cttgctctta ctacttttaa tttctttcca ttccccttac gacgtgtcat aggtacttca
     6061 attcttttac gtccacttaa ataagctccc gtaatcgaat tttcattctg cataatttct
     6121 tctggtgttc cagccgcaac aatttgaccg ccatgaacac cggcaccagg accaacatct
     6181 acaatataat cacttgcacg cattgtatct tcatcgtgtt ctacgacaat caaagtgttt
     6241 cctaaatctc gcatatcttt taaagcacca atcaaacgat cattatctct ttgatgtaac
     6301 ccaatactag gttcatctaa aacatacata accccagtta aacgtgatcc aatctgtgtt
     6361 gccaagcgaa tacgttgcgc ttcccctcca gatagagtag atgctaaacg atccagtgtt
     6421 aaataactta gcccgacatg atttaagaat gaaaggcgat tttttatttc tttaatgacc
     6481 aattctgcaa tcttagcttg tgttggcgtt aattctaatt cattcataaa ctgaatggct
     6541 tcttgaatac tcatttgtgt ccattcataa atattttttc caccaacaag tacagataga
     6601 gcttttttat ttaaacgtgc cccatgacat tttggacatt gagcatctgc caaaaatgat
     6661 ccataccatt cacgagacat agttgaagtt gtttccatat atcttctttc gatcatagga
     6721 caaacacctt caatatattc aaactttgtt gaaaccgtac cagaacgaga attcaattga
     6781 tatgcgattg gcacgtctga accatataat aaataattta atttcttttt aggtaattcc
     6841 ttgatgggtg tgtccatatc aatctcataa tatttaatta aagcatgaat tcgttgccac
     6901 tcaagatttt ctgtattcac aatatttttg taataatgaa taccgccttg cgcaattgat
     6961 tttgtatcat ctggaattaa taaagacaaa tccacctttt gggtaatacc taaaccttta
     7021 cattctggac atgctccaaa tggtgcattg aacgaaaata attttggttc taatttaggt
     7081 acggaaaagc cacaaatttt acatgcataa ttgctagaaa ataaggtttc tgattcattt
     7141 gttaataaaa tagccaaacc atcacttaat tttaatgcgg tttctaacga atcatgaaaa
     7201 cgtgaacgat cgtcactttt tacaattcga tccactacaa catcgatcgt atgttttttg
     7261 tttttatcta aaggctcaag ttcttcaaga tatttgattt cgccatcaat acggacgcgg
     7321 atatatcctt ctttcattaa cttttctatt aaatccttat gagtgccttt tttcccttgt
     7381 ataacagggc ttaaaatagt acatctagtt ttatcaggca attccatgat tttatcaatc
     7441 atttccttaa ttgtatttcc agtaatcgga atgtggtggt caggacaata agggactcct
     7501 atacgtgcat ataatagacg taaataatca taaatctctg taacggttcc tacagtagat
     7561 cgtgggttgt tagatgttgt tttttgatca atcgcgatgg ctggtgataa tccatcaatt
     7621 tggtcaacat ctggtttttc actatttcct aaaaactgcc ttgcataagc agataaggat
     7681 tccatatatc gacgttgtcc ttctgcatat attgtatcaa aagctaaaga agtttttcca
     7741 cttcctgaaa cgccagtcat gatgattaat ttatcttttg gtatttttaa acttatattt
     7801 ttaaggttgt tttcacgagc accttgtatt gttatatact tttggtccat aataaacctc
     7861 ctatgcacac tattatacca taaacatttg ttcattattt gtgaaataat atgcaagaaa
     7921 aaaggcaaaa aaaaaggacc cggcagctag ctatcttcgc aggggcaagc ccaactattg
     7981 tcgccgtgaa agtgcttaac ttctgagttc gggatga
//
LOCUS       DBMDWL010000094         7957 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3342_length_7957_cov_2.260056, whole genome shotgun sequence.
ACCESSION   DBMDWL010000094 DBMDWL010000000
VERSION     DBMDWL010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3342_length_7957_cov_2.260056"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="ACZPDN_07280"
     CDS             complement(<1..204)
                     /locus_tag="ACZPDN_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298461.1"
                     /translation="MKEKLYRFMQGRYGTDKLNQFLFYVELILLVLSLFFRRNIVINI
                     LFYISIIIYLARALSKNYVARSIE"
     gene            373..1704
                     /gene="gdhA"
                     /locus_tag="ACZPDN_07285"
     CDS             373..1704
                     /gene="gdhA"
                     /locus_tag="ACZPDN_07285"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HIR1298462.1"
                     /translation="MKDLEQLYETVKKRNPNDKEFLQAVEEVFESLNIIADVHPEELD
                     DDVLERLVEPERQIIFRVPWVDDNGKTQVNRGYRVEFNSAIGPYKGGLRFHPSVNISI
                     IKFLGFEQVLKNSLTTLPMGGGKGGSDFDPKGKSDREVMRFCQSFMSELYRHIGPNTD
                     VPAGDIGVGGREVGYLFGQYKRLKNEYSGVLTGKGLTFGGSLIRTEATGYGLCYFTQA
                     LLKDNGTSFEGKTVTISGSGNVAIYACEKATQLGAKVVTMSDSNGFVYDPEGIDLDLV
                     KDIKEVRRGRIKEYVEKRPNATYTPNARPWTVACDIALPCATQNELDLEDAKALVANK
                     VFAVCEGANMPTTPEAIHYLMKNGVFYAPGKASNAGGVACSGLEMSQNAQHLSWTAEE
                     VDQKLENIMVNIFETCRDTAKEYGHEKEYVVGANIAGFLKVAKAMKAQGTV"
     gene            1787..2458
                     /locus_tag="ACZPDN_07290"
     CDS             1787..2458
                     /locus_tag="ACZPDN_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HIR1298463.1"
                     /translation="MKRAKQTSESIELGILLALSGGFMDAYSYLARGQVFANAQTGNM
                     LLFGVNLARGQFQHALHYLCPVLAFGLGIFLAEFVHFQKIQKVHWRQVTLLIEIIILF
                     GVGYISFEQNLLANSLTSFACGLQVQAFRKIHGKGYATTMCIGNLRTGTHEMCNYLCT
                     KKVQHFQSGLLYYSIILTFIIGAILGNFCIQIFSAKAIWISVSLLIVAFILMFVDREK
                     DEAFQ"
     gene            complement(2412..3011)
                     /locus_tag="ACZPDN_07295"
     CDS             complement(2412..3011)
                     /locus_tag="ACZPDN_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012521.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1298464.1"
                     /translation="MTHFYIIRHGETVFNRKGRIQGWCDSPLTDLGVSQAKQLGKELK
                     NIPFDVCFCSTSERAIDTANSILEGRNVKVIPSKNLKEQSFGDFEAEKSVDIFKDGVR
                     FPDGYRFCGGENHSDVIERVTNELKKIASEYPNANVLVVCHGSAIKHIVNALCPGFVD
                     EKPNTAALVPNCSVTRMDCDTELKLIEKPHLFHGQQTLK"
     gene            3255..3818
                     /locus_tag="ACZPDN_07300"
     CDS             3255..3818
                     /locus_tag="ACZPDN_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR1298465.1"
                     /translation="MDFITILFIGVGLAMDACAVSLAKGMSLDKDKVKKYALILALAF
                     GFFQALMPAIGYFAGSHFADYIQSIDHWIAFILLSLIGINMIKEGKEEKEDEVKIHSI
                     SFKDILLLAIATSIDALAVGVSFAFLKVNLFIATTTIGITTFVLSFICVLFGKTLGNL
                     FEKYAEYMGGSILILLGIKILIEHLFF"
     gene            complement(3826..4335)
                     /locus_tag="ACZPDN_07305"
     CDS             complement(3826..4335)
                     /locus_tag="ACZPDN_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1298466.1"
                     /translation="MMKKTLILGDSNTWGYDPRGYFQRYDLTYVDYLNDLVAGWMFFE
                     DSLNGRLLRDVKDETYDLASIDLFCVMLGSNDLMHYYNVDQIVSFMHELIDSIDTRKV
                     LILCPPILQIDIFKVESIRLNEAYKKMNVRCIDCNPLNMSFDGVHLSEKGHKEFALKM
                     AEYMKKEFC"
     gene            complement(4332..5675)
                     /locus_tag="ACZPDN_07310"
     CDS             complement(4332..5675)
                     /locus_tag="ACZPDN_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298467.1"
                     /translation="MRSYQVDMCNGPILKKLIVFALPLILSGCLQLLFNAADIIVVGR
                     FTGNQALAAVGSTSALINLLVNMFIGISVGANVVLGKSIGARDEENTSKAVHTAIFIA
                     VFGGFLMVFVGFFFAKPLLELMATPIDVIDLSSLYMRIYFAGMPFFMVYNFGAAILRS
                     IGDTKRPLYFLMVSGIINVLFNLCFVIVFHMGVSGVALATIISEGISAGLILSCLKHM
                     DGPLHFELKDMRFHKELALKMLEVGLPAGLQGIIFSISNVLIQSSINSFGSLVMAGNT
                     AASNIEGFVYTSMNAVYQTSLSFTSQNFGARKYDRIDKILLECLAIVTVVGLVLGQGA
                     YFFGQQLLSIYSSDKSVIQFGIDRLAIISTMYCLCGIMDTLVGSLRGVGYSILPMLVS
                     LTGVCVIRVIWIFTVFKSVHTTFSLYISYPITWVITLVAHGICFMVVRKRIRRVA"
     gene            complement(5765..6820)
                     /locus_tag="ACZPDN_07315"
     CDS             complement(5765..6820)
                     /locus_tag="ACZPDN_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR1298468.1"
                     /translation="MDVVEILKKAIKENASDIFFVAGSPCMLKVGQQLVAITQEKMMP
                     NDTKNIVQQLYGFAPYCSYENFEKNGEDDFSFSLSQVGRFRVNVYRQRNSEAAVLRVV
                     KFELPKPEDFHIPESILSLSNRRKGIILVSGPAGSGKSTTLACLIDRMNETRNCHIIT
                     IEDPIEFLHSHKKSIVSQREVYHDTKNYLTALKSALREAPEIILLGEMRDEETVSTAL
                     SAAETGHLILSTLHTVGAVNTIDRIVDMFQGHQDQVRSQLSMILEAVISEQLIPTIDG
                     ELIPVFEIMQVNPAIRSQIRENKIHQIENTMASNRQNGMVMMDDSIFELYQQNKISKD
                     IAIQYSLHPDRMKSRVQ"
     gene            complement(6822..7115)
                     /locus_tag="ACZPDN_07320"
     CDS             complement(6822..7115)
                     /locus_tag="ACZPDN_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298469.1"
                     /translation="MKQSRIGLGVVTMLSIFVVVCMCVLVLFSSNKVYQMHEQVNRSY
                     AYKKAYYQAEIKAHKLIDQNETDFLVHVKEDTYLKVKVDHGQIVVFKTIVKGE"
     gene            complement(7112..>7381)
                     /locus_tag="ACZPDN_07325"
     CDS             complement(7112..>7381)
                     /locus_tag="ACZPDN_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298470.1"
                     /translation="FLITSTILIEIYAKMMQVSKEDTYRQEAMIIAQNEIETHTRVGD
                     YTRKMNDTVYDIKIKRINDNEFRIQIYVKETKVLDYHYYQEGNIK"
     assembly_gap    7382..7391
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7397..7840)
                     /locus_tag="ACZPDN_07330"
     CDS             complement(7397..7840)
                     /locus_tag="ACZPDN_07330"
                     /inference="COORDINATES: protein motif:HMM:NF027478.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HIR1298471.1"
                     /translation="MMKNAGVFFSLSLMLLFVLCCFMLLNIQIEDYSMLQRRTKNDFE
                     TYTPVLYVTNKIHSYDEMNGVSVENRDGQKMIKLSDTKSDTYLYRDKSKLMELCVIKD
                     MQPDFSTGQALMACDDFDAKQDENKIICEINGVEFYVELRSGVAS"
BASE COUNT         2739 a         1488 c         1113 g         2607 t
ORIGIN      
        1 ttcaattgat cttgccacat agttcttcga taatgcacgt gctaaataaa taataatact
       61 aatatagaac aatatattga tcacaatatt tcttctaaaa aataaactta atactaataa
      121 aataagttct acataaaata ggaactgatt taatttatcc gtaccatatc taccttgcat
      181 aaaacgatat aatttttctt tcattccaat caccgagtac tatcatagca tacaaaatat
      241 gaacttacta tgtgcatttt accatcattt gtatacttgt ttttattgtt tacaaattat
      301 tttcatattt ttatgaaaca ttttcaattt ttgtgctata atcatcacgt attaaataag
      361 ggaggactta aaatgaaaga cctagagcaa ttatacgaaa ccgtaaaaaa acgaaatccc
      421 aatgataaag aatttcttca agctgttgaa gaagtctttg aatcattgaa tattatcgcc
      481 gatgtacatc cagaagaatt agacgatgat gtattagaaa gacttgtaga acctgaaaga
      541 caaatcattt tccgtgttcc ttgggttgat gataatggca aaactcaagt aaaccgtggc
      601 taccgtgttg aattcaactc tgccatcgga ccttacaaag gaggtttacg attccaccca
      661 tctgtaaaca ttagtatcat caaattctta ggatttgaac aagtattaaa aaattcattg
      721 actacacttc ctatgggagg tggaaaaggt ggatctgatt tcgatccaaa aggaaaaagt
      781 gatcgcgaag ttatgcgttt ttgccaaagt ttcatgagtg aattataccg ccacatcgga
      841 ccaaacactg atgttcctgc aggagatatt ggtgtcggag gacgtgaagt tggatattta
      901 tttggacaat acaaacgttt gaaaaacgaa tatagcggtg tcttaactgg taaaggatta
      961 acattcggtg gatccttgat tcgtactgaa gccactggat atggtctatg ttactttaca
     1021 caagctttat taaaagataa tggaacaagt tttgaaggaa aaactgttac catttcagga
     1081 agcggaaacg tagctatcta tgcttgtgaa aaagcaacac aacttggagc aaaagttgta
     1141 accatgtccg attcaaacgg attcgtttac gatccagaag gaattgattt agatttagtt
     1201 aaagacatca aagaagttcg tcgtggacgt attaaagaat atgttgaaaa aagaccaaat
     1261 gcgacataca caccaaatgc tcgtccatgg actgttgcat gcgatatcgc ccttccatgt
     1321 gccactcaaa atgaacttga tttagaagat gcaaaagcat tagtcgcaaa taaagtattc
     1381 gcagtatgcg aaggggctaa catgccaaca acaccagaag ctatccacta tcttatgaaa
     1441 aacggtgtat tctatgctcc aggtaaagca agcaatgccg gaggtgttgc atgttctggt
     1501 cttgaaatgt cacaaaatgc tcaacactta tcttggacag cagaagaagt tgatcaaaag
     1561 cttgaaaaca ttatggtaaa tattttcgaa acttgtcgtg atactgctaa agaatatggt
     1621 catgaaaaag aatatgtagt tggagctaac attgctggat tcttaaaagt tgcaaaagca
     1681 atgaaagcac aaggcacagt ttaatgaata taaaagccga tttcggcttt tattttttgt
     1741 gattttgtct tattatctta aaagaaaaaa aggttgtgat tattttatga aaagagcaaa
     1801 acaaacatct gaatccattg aacttggtat tcttctagcg ttatccggag gttttatgga
     1861 tgcctattca tacttggcaa gaggacaagt ctttgcaaat gcccaaactg gaaatatgct
     1921 cctctttggt gtcaatttag caagaggaca attccagcat gctctacatt atttatgtcc
     1981 cgtacttgct tttggtctag gtattttctt agcggaattt gtacattttc aaaaaattca
     2041 aaaagtacac tggcgacaag ttactctctt gattgaaatc attatattat ttggcgttgg
     2101 atatatttca ttcgaacaaa accttctcgc aaattcttta acttcttttg cgtgtggctt
     2161 acaagtacaa gcctttcgta aaatacatgg taaaggttac gcaacaacga tgtgtattgg
     2221 aaatttaaga acaggtacac acgaaatgtg taattattta tgcacaaaaa aagtccaaca
     2281 ttttcaaagt ggacttctct actactcaat tattttaaca tttataatcg gtgccattct
     2341 aggaaacttt tgtattcaga ttttttcagc aaaagctatc tggataagcg tttcactatt
     2401 aatagtcgcc tttattttaa tgtttgttga ccgtgaaaaa gatgaggctt ttcaataagt
     2461 tttaattctg tatcacaatc cattctcgtc accgaacaat ttggcactaa agctgctgta
     2521 ttcggttttt catctacaaa acctggacac aatgcgttca caatatgttt gatggcagaa
     2581 ccatgacaaa ctactaaaac attcgcattc ggatattcgg atgcgatttt ttttaattca
     2641 ttcgttactc tttctataac gtcactatgg ttttcaccac cacaaaaacg ataaccatct
     2701 ggaaaacgta ctccatcttt aaatatatcc actgattttt cagcctcaaa atcaccaaaa
     2761 ctctgctcct ttaaattttt agaaggaata acctttacat ttctaccctc taaaatagag
     2821 tttgccgtat ctatagctct ttcactcgtt gaacaaaaac atacatcaaa tggaatattc
     2881 ttcaattctt tacccaattg ttttgcctga gaaacaccca aatccgtcaa cggtgaatca
     2941 caccatcctt gaatacgacc tttgcgatta aatactgttt ctccatgtct aataatataa
     3001 aaatgagtca tatatacacc ttccttttgc ctcattataa tccatttcag ttagatatca
     3061 ataactattt ttcattgtaa agaaaatttg attttgatac tataaaccca tccataaggg
     3121 agtagttatc ttacttctgt aagtatttaa gtcaacatac tgatcaaaag atctggctta
     3181 ttgtaaaaaa caagacttat gcgaaaaaag atatcttttt tcgtgtaagt cttttcttgt
     3241 tttaaggagg aaatatggat tttattacta ttttatttat tggcgttggt ttagctatgg
     3301 atgcatgcgc cgtttcatta gcaaaaggca tgagccttga taaagataaa gtaaaaaaat
     3361 acgctttgat cttggcactc gcatttggat tctttcaagc acttatgcca gctattggct
     3421 attttgcagg aagtcatttt gccgattata tccaatccat cgatcactgg attgccttta
     3481 ttttgttgag cttaataggt atcaacatga ttaaagaagg aaaagaagaa aaagaagatg
     3541 aagtaaaaat tcactccatc tcatttaaag acattcttct tttagctatc gccacaagta
     3601 ttgatgcctt agctgtaggt gtaagttttg cctttttaaa agtaaattta ttcattgcca
     3661 caaccacaat aggaattaca acctttgtac tttccttcat ttgtgtattg tttggcaaaa
     3721 cactagggaa cctatttgaa aaatatgcag aatatatggg cggttctatc ttaattcttt
     3781 taggtatcaa aatcttgatt gaacatttat ttttctaaca agacttcaac agaattcttt
     3841 tttcatatat tccgccatct tcaaggcaaa ttctttatgt cccttttcag ataagtgcac
     3901 accatcaaaa ctcatattta atggattgca gtctatacaa cgaacgttca tttttttata
     3961 ggcttcattc aatcgaatac tttccacttt aaatatatca atttgtaaaa ttggtggaca
     4021 aagaatcaat acctttcttg tatctataga atcaattaac tcatgcataa aagaaacgat
     4081 ttggtccaca ttataataat gcatcaaatc attcgatcca agcatgacac aaaacaaatc
     4141 gattgaagct aaatcataag tttcgtcttt aacatccctt aacaaacgtc catttaatga
     4201 atcttcaaaa aacatccatc cagctactag atcattcaag taatctacgt aagttaaatc
     4261 atatctttga aaatatcccc ttggatcata tccccaagta ttagaatccc ccaaaatcaa
     4321 tgtttttttc atcatgccac tcttcgaatt cgtttacgca caaccatgaa acaaattcca
     4381 tgtgctacaa gcgttataac ccatgtgatt ggataagaaa tatacaatga aaatgttgta
     4441 tgcactgact taaatacagt aaatatccat ataacccgaa tgacacaaac acctgttaaa
     4501 gataccaaca ttggtagaat tgaatagcca actcctctta aacttccaac taaagtgtcc
     4561 ataatcccac ataaacaata cattgtacta atgatggcta atcgatcaat accaaattga
     4621 ataacacttt tatcactcga ataaatagat aataattgtt gtccaaagaa ataagcacct
     4681 tgtcctaaca caagaccaac taccgttaca atagctaaac attccaataa tattttatcg
     4741 attcgatcat attttcttgc tccaaaattt tgtgaagtaa atgacaaaga agtttgataa
     4801 acagcattca tagacgtgta cacaaagcct tcaatattac tagccgctgt gtttccagcc
     4861 ataactaaag atccaaaaga gttaatactt gattggatca aaacatttga aatactaaaa
     4921 ataatgcctt gaagtcctgc aggaagtcct acttctaaca tttttaatgc caattcttta
     4981 tgaaagcgca tatcctttaa ttcaaaatgt aaagggccgt ccatatgctt taaacaagac
     5041 aaaataagtc ccgcactgat tccttcagat atgattgtag ccaatgcaac accagaaact
     5101 cccatatgga atacaattac aaaacataaa ttaaataata cattaataat tccagaaacc
     5161 attaagaaat ataatggacg ttttgtatcc ccaattgacc taagtatagc agcaccaaag
     5221 ttatagacca taaaaaaagg catccctgca aaataaatcc gcatatataa agaagataaa
     5281 tcaattacat ctattggtgt tgccatcaat tctaataaag gtttagcaaa aaagaaacct
     5341 acaaatacca ttaagaatcc tccaaaaaca gcaataaata tagctgtatg tacagcttta
     5401 gatgtatttt cttcatctcg agcaccaata gattttccca atactacatt tgcaccaaca
     5461 ctaatcccaa taaacatatt gactaataaa ttgatcaatg cacttgttga accaaccgct
     5521 gcaagtgctt gatttccagt aaatcttcca accactataa tatcagccgc attaaataaa
     5581 agttgtaaac atcccgataa tattaatggt aacgcaaaaa caatcaattt ttttaaaatt
     5641 ggaccattac acatatccac ctgataacta cgcattaaaa atctcccatt caataataga
     5701 caaattatat acctactgta aaattattga aagaaaaaaa gaatgaaatg taatttttca
     5761 ttctttattg cactctactt ttcatacgat ccggatgtaa actatattga atcgcaatat
     5821 cttttgaaat tttattctgt tgatacaatt caaaaattga atcatccatc ataaccatac
     5881 cattttgtcg attggatgcc attgtatttt caatttgatg aatcttattt tcacgaatct
     5941 gagatcgaat agccggattt acttgcataa tttcaaatac tggaattaat tctccatcga
     6001 ttgtaggaat caattgttca cttataaccg cctctaagat catggacaat tgactacgaa
     6061 cttgatcttg atgtccctga aacatatcta caatacgatc tattgtattt actgcaccaa
     6121 ctgtatgtaa tgtggataaa attaaatgtc ctgtttccgc agcagaaagt gctgtagaaa
     6181 ccgtttcttc atcacgcatt tctcctaata aaataatttc aggtgcttca cgcaaagccg
     6241 attttaatgc cgttaaataa ttctttgtat catgatacac ttcgcgttgt gaaacaatcg
     6301 atttcttatg cgaatgcaaa aattcaattg gatcttctat cgtaataata tgacaatttc
     6361 tagtttcgtt cattcgatca atcaagcaag ctagagttgt actctttcca cttccagcag
     6421 gaccacttac taaaataatc ccttttctac gatttgaaag acttaaaata gattctggta
     6481 tatgaaaatc ttctggttta ggaagttcaa atttaactac acgcaaaaca gcggcttctg
     6541 aatttctttg acgatatacg ttaaccctaa aacgtcctac ttgtgataac gaaaacgaaa
     6601 aatcatcttc cccatttttt tcaaaattct catatgagca ataaggtgca aagccataca
     6661 actgttgaac aatatttttc gtatcattcg gcatcatttt ttcttgtgtt atcgcaacaa
     6721 gctgttgtcc taccttcaac atacaaggac ttccagcaac aaagaaaata tctgatgcat
     6781 tttcttttat agcttttttt aaaatctcaa caacatccat actattctcc tttcacaatc
     6841 gtcttaaata caacaatttg accatgatca accttcactt ttaaatatgt atcctctttt
     6901 acatgaacca aaaaatccgt ttcattttga tcaatcaatt tatgtgcttt aatctcagct
     6961 tgataatatg cttttttata cgcatagcta cgattgactt gttcatgcat ttgataaact
     7021 ttattcgatg aaaacaacac taaaacacac atacatacaa cgacaaatat acttagcatt
     7081 gtaacgacac ctaatcctat tcttgactgt ttcatttgat gttcccctct tgataataat
     7141 gataatctaa tacttttgtt tctttaacat aaatctgtat acgaaactca ttatcattaa
     7201 tacgcttgat tttaatatca taaaccgtat cgttcatttt tctagtataa tctccaacac
     7261 gagtatgcgt ttcaatttca ttttgagcaa taatcatagc ctcttgacga tatgtatctt
     7321 ccttagatac ttgcatcatt ttcgcataga tttcaattaa aatcgtggat gtgataagaa
     7381 annnnnnnnn nctttttcat gatgccaccc cacttcttaa ttcaacataa aactcaacac
     7441 cattaatttc acaaataatt ttattttcat cttgcttcgc atcaaagtca tcacatgcca
     7501 ttaaagcttg tcccgtacta aaatctggtt gcatatcttt aattacacaa agctccatta
     7561 acttagattt atctctatat aaatacgtgt cactttttgt atccgatagc ttaatcattt
     7621 tttgaccatc tctgttttca acactaactc cattcatttc atcataacta tgaattttgt
     7681 ttgtgacata taaaacgggt gtatatgttt caaaatcatt tttagtacgc ctttgcagca
     7741 tcgaataatc ttcaatttga atatttaaaa gcatgaaaca gcataacaca aacaataaca
     7801 ttaacgaaag agaaaagaaa acacctgcat ttttcatcat tcattcccct ttctataaac
     7861 ataaatattt ggatatatat tatccccctg ccaatcatat gtaatgaaat aactatttat
     7921 atcataagta aacccataat atttttttaa atattga
//
LOCUS       DBMDWL010000095         7905 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3386_length_7905_cov_2.055100, whole genome shotgun sequence.
ACCESSION   DBMDWL010000095 DBMDWL010000000
VERSION     DBMDWL010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_7905_cov_2.055100"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(149..484)
                     /locus_tag="ACZPDN_07335"
     CDS             complement(149..484)
                     /locus_tag="ACZPDN_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298472.1"
                     /translation="MLGSLHLINNVFESICKAVDEVFAERTIDDFIAERMLIFISALV
                     KEGYDVSVLQQLNVRDISFPVIVNVLGITDVKRLAHLFMAFTQRFYGAALPMRQNYDL
                     IRRWLNGSV"
     gene            504..1937
                     /locus_tag="ACZPDN_07340"
     CDS             504..1937
                     /locus_tag="ACZPDN_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1298473.1"
                     /translation="MPKQGWINTYVMISLAVASILIDFLVTAAIAQFEIRHRKDLEYA
                     SQIEWQAKKAQEASAAKSRFLFSMSHDIRTPMNAIMGYTELMEKNVGNIEKEKDYLSK
                     IHSSSKFLLDLINSILEMARIESGKETLNIKACNIFDVLDSLNSVFEKQAEQKGLTYQ
                     CTTKIEHPYVYCDQIKFEEILLNVVGNSVKYTKNGNISIHVEEVESGQFQCMIQDTGI
                     GMSEAYLLHAFEDFSREKSGTQTSVKGTGLGLSIVKSLVELMHGTIEISSQVNQGTTT
                     RMKFHFEIAKESELEKNQETNIIDLKGKHILLAEDNDLNAEIAMTLLFDYGLIVDHVS
                     DGIACVKQVKEKEYDVVLMDIQMPNMDGYQATQKIREFSDIPIVAMTANAFEEDKQKA
                     LSIGMNGHIAKPIDMDKVIKTLSNVFVFKCPVCGKYTFQSGPGSYEICPVCGWEDDKA
                     QYKDPNLKGGANKLSLKEYKERYEKNH"
     gene            1921..2298
                     /locus_tag="ACZPDN_07345"
     CDS             1921..2298
                     /locus_tag="ACZPDN_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298474.1"
                     /translation="MKRIINKYLLLFLCIFSLVLPTGCEIKEQKQVVVDYQEYHFRNE
                     DLLESHYEKHGKEMGFSSSKEYEMSASDVVNNPESLHKTEKEDGDDVYYKENTNEFVV
                     VSTDGYIRTYFNPDAGRKYFDRQ"
     gene            complement(2353..2895)
                     /locus_tag="ACZPDN_07350"
     CDS             complement(2353..2895)
                     /locus_tag="ACZPDN_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1298475.1"
                     /translation="MMNSITKKLVSCALCIALGVLLPMAFHVIPNAGSIFLPMHIPVL
                     ICGLFCGWPYGLACGIITPFLSSVTTGMPPAMMLPQMMIELAVYGLVAGLCTQYISMK
                     NEMTKLYASLIIAMLAGRIVNGLVNTCILSTQGYTLSVFMMASFITCLPGIILQLIVI
                     PVLVRILNRNIYKQPEKVLA"
     gene            complement(2951..3607)
                     /locus_tag="ACZPDN_07355"
     CDS             complement(2951..3607)
                     /locus_tag="ACZPDN_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1298476.1"
                     /translation="MDNEYLLNFLKEKNVPIIQKKRHTYLAYYGLEQQDTYVLKEGVI
                     KTSIILHDGREFNISYINTPDIISLLKDEVSQYTSAPFNVRVESDVATFYRIPRVLFW
                     SYVNQDQKLQDYVNAYYREKLTEAIFRQQLMMMNGKTGAICSFLYQLIHLFGRKVEAG
                     ILIDFQITNDDIAGFCGISTRNSVSRILCELKKEGVIKIVNQKILILDIDYLKQYVQC
                     "
     gene            3742..5019
                     /gene="larA"
                     /locus_tag="ACZPDN_07360"
     CDS             3742..5019
                     /gene="larA"
                     /locus_tag="ACZPDN_07360"
                     /inference="COORDINATES: protein motif:HMM:NF033504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HIR1298477.1"
                     /translation="MFEMNVPYDKGQMKIKLEDKNLAGVLEGRQSDYKTTLSENEIVE
                     QSLDNPIGSKSLEELAKDKKNIVIISSDHTRPVPSKIITPILLRRIRSVSPDARIRIL
                     VATGFHRPSTHEELVAKYGEEIVKNEEIVMHVSTDDSSMVKIGQLPSGGDCIINKVAA
                     EADLLISEGFIEAHFFAGFSGGRKSVLPGIASYKTIMANHSGEFINDKKSRSGNLQHN
                     LIHEDMVYAARTAKLAFIVNVVLNGNHEIIGSFAGDMEKAHEKGCDFVRSLASVNKVT
                     CDIAISTNGGYPLDQNIYQAIKGMTAAEATLNPDGIIIMIAGCRDGHGGIGFYHNIAD
                     VKDPEEFEQKAIHTPRLETVPDQWTSQICARILAHHKVILVSDLVDPQLVKDMHMDLA
                     TTVDEALEKAFEIKGKDAKVAVIPDGLGVVVNM"
     gene            5038..5748
                     /gene="larD"
                     /locus_tag="ACZPDN_07365"
     CDS             5038..5748
                     /gene="larD"
                     /locus_tag="ACZPDN_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D/L-lactic acid transporter LarD"
                     /protein_id="HIR1298478.1"
                     /translation="MLHNLIAEFLSTGLMILFGVGVHCDEVLTNTKYHGSGHIFAITT
                     WAFGISVCLYIFGGVCMNPAMVLAQCVLGMLPWTSFIPYVIAEFLGALVGALICYVMY
                     KDHFTESEGNVNPIAIRNIFSTNPNCRNLPRNFFVETLATTVFLSAILAVATKYETQL
                     PIGVGLIVWAVGMGLGGTTGFAMNQARDLGPRLAFQLLPIKNKANNDWQYGLLVPGIA
                     PFVGALLAALFCHLFLGI"
     gene            complement(5797..7089)
                     /locus_tag="ACZPDN_07370"
     CDS             complement(5797..7089)
                     /locus_tag="ACZPDN_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298479.1"
                     /translation="MLKQYLKFVIPSICSMVIFNLYTLVDGIFVAHFEGDTALASINV
                     ASPYVTTIFSIGVLFAIGSSTVMSIYRGANEERKMHEVFSMNTITISCVALCIAILSF
                     INSHHITEFLGANEDVFNYTDTYIKVLSCFSFFYIVSYCFEVLLKADGFPQKSIMGIC
                     LGAGTNLVLDPLFMGVFNMGISGAALATGLSQLVTFCFFFYHFAFSHKSHFHFVKANY
                     PWHEYSRILSLGLPDCISEISPGIMITVFNYRINQLLGTNGLITFTVLMYIYNLVLMV
                     MVGTSQGTQPLVSYYYGRSDKKAVSTFHKYARVLVSCAGVLLFLIVEIFAPNITAAFI
                     LVNTENYFNTITALRQFSFVFLIMGHVVITSGYMVALEEPRKAFVLSISRGILFVLLS
                     LFVCTTLFKENGIWISCTVSEIFCLAIALYDLKTQKKI"
     gene            7147..>7905
                     /locus_tag="ACZPDN_07375"
     CDS             7147..>7905
                     /locus_tag="ACZPDN_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1298480.1"
                     /translation="MKKYYKINEIAKLYNIKTDSLRYYEEVGLICPMRSKSNYRLYTL
                     NDIYILNIIRDCLKLGYDTSQIKEYLDNRSVNNTIAFLKEEEKLIQRQIQELNTKLLS
                     IQTRIIDLDRTKQIDFNTCKIEAYPKRYCRYLKEKIDQDEKIDFLLTKLSESMEEDIS
                     VLGNMDSGSVVEYKNNAYVYTSVFILTQEEKHDFILDEGIYCTYTYSGEYDRTNQLFY
                     KMKDWIQEHNYKIEGPFLEFLLIDIHETKKVEEYI"
BASE COUNT         2837 a         1246 c         1410 g         2412 t
ORIGIN      
        1 atcactatta atgttcacta caggtttatt gtcatcacct gaattattat cgcttgtaat
       61 actaccattt ccaaacgact gcatagcatc aactttctta cctgcagaaa taccaccttc
      121 atcacctgtt gaagatttta catcaaaatc aaacagatcc attcaaccac ctccttataa
      181 gatcataatt ctgtcgcata ggcaaagctg ctccataaaa tctctgagta aaggccataa
      241 ataaatgtgc taatcgtttc acatcagtta tgcctaatac atttactatt acaggaaatg
      301 aaatgtcacg tacatttaac tgttgtaaaa cgcttacgtc gtaaccctct ttaaccaacg
      361 cagaaataaa tatcaacatt ctttccgcaa taaaatcatc tattgtcctc tcggcaaata
      421 cctcatctac tgctttacat atagattcaa atacattgtt gatcaagtgt aagcttccca
      481 acaataaatg gaattttgaa attgtgccaa aacaaggttg gatcaataca tatgtaatga
      541 taagtttagc tgtcgctagt attttgatcg attttctagt aacggccgct attgctcaat
      601 ttgaaattcg ccatcgaaaa gatttagagt atgccagtca aattgaatgg caggcaaaaa
      661 aagcacaaga agcaagtgct gccaaatctc gtttcttatt tagtatgtca cacgacattc
      721 gtacacctat gaacgcaatt atgggatata cggaattaat ggaaaagaat gttggaaata
      781 ttgaaaaaga aaaggattat ctatccaaga ttcactcttc tagtaaattt cttttagatt
      841 taattaactc cattttagaa atggcacgca tcgaaagtgg aaaagaaaca ttgaatataa
      901 aggcatgtaa tatatttgat gtgttagatt ctttgaattc agtctttgaa aaacaagctg
      961 aacaaaaagg cttaacctat cagtgcacca caaagattga acatccatat gtgtattgtg
     1021 atcaaattaa gtttgaagag atattgttga acgtggttgg aaattccgtg aaatatacta
     1081 aaaatgggaa tatttcgatt cacgttgaag aagtagaatc tggccagttt caatgcatga
     1141 ttcaagatac aggtattgga atgagcgaag cgtatttgct acatgccttt gaagactttt
     1201 ctagagaaaa atcaggtacg caaacgagcg taaaaggtac aggacttggc ttatcgatag
     1261 taaaatcttt ggttgaattg atgcatggta caattgagat ttcaagtcaa gtcaatcaag
     1321 ggacaacaac tcgaatgaaa tttcattttg aaattgccaa agaaagtgag ttagaaaaaa
     1381 atcaagaaac gaatattata gaccttaaag gaaaacacat tttactagct gaagacaatg
     1441 atttaaatgc agaaattgcg atgacacttt tatttgacta tggattgatt gtagatcacg
     1501 taagtgatgg aattgcttgt gtaaaacaag tcaaagaaaa agaatacgat gtagttttaa
     1561 tggatattca aatgccaaat atggatggtt atcaagcaac acaaaagatt cgtgaattta
     1621 gtgatattcc aattgtggcg atgacggcca atgcctttga agaggataaa caaaaagcat
     1681 tatccattgg aatgaacgga catatcgcaa agccaatcga tatggataaa gttattaaaa
     1741 cactatcaaa tgtttttgtg tttaaatgtc ccgtttgtgg aaaatataca tttcaatctg
     1801 gaccaggaag ctatgaaatc tgtccagtat gtggatggga agatgataaa gcgcaatata
     1861 aagatccaaa tttaaaaggt ggagccaata aactaagttt gaaggagtat aaagaacgat
     1921 atgaaaagaa tcattaataa atatttgctc ttatttcttt gtatattctc acttgtactt
     1981 ccaacaggat gtgaaattaa ggaacaaaaa caagtagtcg tagactatca agaataccat
     2041 tttcgtaatg aagatttgtt agagtctcat tatgaaaaac atggtaaaga aatgggattt
     2101 tcatcatcaa aagaatacga aatgagtgcc agtgatgtag tcaataatcc agagtcctta
     2161 cataaaacag aaaaagaaga tggagatgat gtatactaca aagaaaatac caatgaattt
     2221 gtggttgtat ccacggatgg gtatattcga acctatttta atccggatgc gggtagaaaa
     2281 tattttgata ggcaatgatg aaaaaaagac cgaatgtcag attaaaacct gaatattcgg
     2341 tctttcttgt cattaagcta aaactttttc tggttgttta taaatatttc tatttaaaat
     2401 acgaaccaaa acaggaatga caatcaattg aaggatgata cctggaagac aagtaataaa
     2461 gctagccatc ataaatacag ataatgtata tccttgtgta ctaagaatac atgtattgac
     2521 taaaccattc acaattcttc ctgcaagcat cgcaataatt aagcttgcat atagtttagt
     2581 catttcattc ttcattgaaa tatattgtgt acataaacca gcaactaagc catataccgc
     2641 aagttcaatc atcatttgtg gcaacatcat tgcaggtggc atacctgttg tcacacttga
     2701 aagaaatggt gtaataattc cacatgctaa accatatggc catccacaaa ataatccaca
     2761 aattaataca ggaatgtgca ttggtaaaaa tatgcttcca gcatttggga tcacatgaaa
     2821 tgccattgga agtaatacac ctaaagcgat gcataaagcg caagatacta gttttttggt
     2881 gatactattc atcattaaat acctcttctt ctaaaaaata cttggtaagg atgtatggac
     2941 agattaaaat ttaacattgc acatattgtt ttaaataatc gatatctaga attaatattt
     3001 tttgatttac aatcttaatg acgccttcct tttttagctc acaaagaatt cgactcacac
     3061 tgtttcgagt ggaaattcca caaaatcctg caatatcatc atttgtgatt tgaaaatcaa
     3121 taagaatacc cgcctctact tttcgaccaa ataaatggat taattgatat aagaaggagc
     3181 atattgcacc tgtttttcca ttcatcatca taagttgttg gcgaaaaata gcttccgtta
     3241 atttttctcg ataatatgcg tttacataat cttgtagttt ttgatcttga tttacataac
     3301 tccaaaataa cactcgagga attctataga atgtggccac atcactttca acacgaacat
     3361 taaatggagc cgatgtatat tgacttactt catcttttaa taaagaaata atatccggtg
     3421 tattgatata agaaatgttg aattcacgac catcatgcag gatgatactt gtcttaatta
     3481 ccccttcttt taacacatag gtatcttgtt gttcaagccc ataatatgct aaataggtgt
     3541 gtcttttttt ctgtataata ggcacatttt tctcttttaa aaaatttaat aagtattcat
     3601 tgtccatatc cgttctcctt taccagtttt ggattataac aatactccaa actttgcgaa
     3661 acaacaaaag ttaacatgaa atgaggtttc acaatttttt aattcggatt atattaattc
     3721 gggaaaaaag gagagataaa aatgtttgaa atgaatgtgc cttatgataa aggtcaaatg
     3781 aaaatcaaat tagaagataa aaacttagct ggtgttttag agggtagaca aagcgattat
     3841 aaaactacac tttcagaaaa tgaaattgta gaacaatcat tggacaatcc aattggttca
     3901 aaaagcttag aagaactagc taaggataag aaaaatattg tcattattag ctcagaccat
     3961 acgcgtcctg taccttcaaa aattattaca cctattcttt taagaagaat tcgtagtgtt
     4021 tcacccgatg cacgtattcg tattttagta gcgacaggat ttcatagacc aagtacacac
     4081 gaggaacttg tagcgaaata tggagaagaa attgtaaaaa atgaagaaat tgttatgcac
     4141 gtatcgacag atgattcttc tatggtaaaa atcggacaat taccaagtgg aggcgactgc
     4201 attatcaaca aagttgcagc tgaagccgat ctattgattt ctgaaggctt tattgaagcc
     4261 cacttctttg caggattttc tggaggaaga aaatcagtgt tgcctggtat tgcttcttat
     4321 aaaacaatta tggcaaatca cagtggtgaa tttatcaatg ataaaaaatc acgttcagga
     4381 aatttacagc acaatttaat ccatgaagat atggtttatg cggcaagaac ggctaaactt
     4441 gcctttattg tcaatgtcgt attgaatgga aatcatgaga ttattggctc ttttgccgga
     4501 gacatggaaa aagctcatga aaaaggttgt gattttgtac gttctttggc aagtgtaaat
     4561 aaagtaactt gtgatatcgc aatcagtacg aatggtggtt atcctttgga tcaaaacatt
     4621 tatcaagcca ttaaaggtat gacagctgct gaagctacat taaatcctga tggtattatc
     4681 atcatgattg caggttgtcg tgatggacat ggaggaattg gattctacca caatattgcg
     4741 gatgtaaaag atccagaaga atttgaacaa aaagctattc atacacctcg cttagaaact
     4801 gttccagatc aatggacatc tcaaatctgt gcacgtattc tagctcatca caaagtaatc
     4861 ttggtttcgg atttagtaga tcctcaactt gtgaaagata tgcatatgga tttagctaca
     4921 actgtagatg aagctttaga aaaagcattt gaaataaaag gaaaagatgc caaagttgcc
     4981 gtcattcctg atggtttggg tgtcgttgta aatatgtaag gaaggaaatt tgaatttatg
     5041 ttacacaatt taattgcgga atttttatca acaggactta tgatcttatt tggtgtggga
     5101 gtgcattgtg atgaagtttt gacaaacaca aaataccacg gaagcggaca catttttgca
     5161 atcacaacat gggcctttgg catcagtgtt tgtctatata tttttggtgg agtctgtatg
     5221 aatcctgcca tggtacttgc acaatgtgtt ttaggcatgc ttccatggac aagctttatt
     5281 ccatacgtta ttgccgaatt tttaggcgca cttgtaggag cattgatttg ttatgtgatg
     5341 tataaagatc attttacaga aagtgaagga aatgtaaatc caatcgccat tcgtaatatt
     5401 ttctctacaa atccaaactg cagaaatttg ccaagaaact tctttgtcga aacactcgca
     5461 acaacagttt tcttgagtgc cattttagct gttgctacaa agtatgaaac acaactacca
     5521 attggagtag gccttattgt atgggctgtt ggtatgggtc ttggaggaac aactggattt
     5581 gcgatgaatc aagctcgtga tttaggacct cgtttggcct tccaattatt acctattaaa
     5641 aataaagcca ataatgattg gcaatatgga ctattagttc caggaattgc tccttttgta
     5701 ggtgcattac ttgctgcttt gttctgccat ttgtttttag gtatttaata ttagaaaaga
     5761 cagatattca aaatcagact gaatatctgt cttttattat attttctttt gtgtttttaa
     5821 atcataaagc gcaatagcta aacagaagat ttcagagacg gtacaactga tccaaattcc
     5881 attttcctta aataatgttg tacaaacaaa tagagataat aagacaaaga gaataccacg
     5941 agaaatcgaa agcacaaagg cttttctagg ctcttctaaa gcgaccatgt agccgctagt
     6001 gatcacgaca tgtcccataa tcaaaaagac aaagctaaat tggcgtaggg ctgtaatggt
     6061 attaaagtag ttttctgtat ttactagaat aaaggcagcc gtgatatttg gtgcaaatat
     6121 ctctacaatt aagaataata aaactccagc acaagagact aaaacacgtg catatttatg
     6181 gaaagttgaa acggcttttt tatcgcttcg accatagtag tagctaacca aaggctgcgt
     6241 tccttgactg gttcccacca tcaccatcaa tactaagtta tagatataca ttaagacggt
     6301 aaaagtaata agtccattag taccaaggag ttgattgata cgatagttaa atactgtaat
     6361 cattatgcct ggagaaattt cagaaataca atcgggaagt cctaaagata gaattctaga
     6421 atattcatgc caaggatagt ttgccttcac aaaatggaaa tgcgatttgt ggctaaacgc
     6481 aaaatgataa aagaagaagc agaaagttac aagttgagat aaacctgtag ctaaggcagc
     6541 accactaatt cccatattga aaactcccat aaataatggg tcaagaacaa ggttggttcc
     6601 agcgcctaga caaataccca tgatagattt ttgtggaaag ccatcggcct ttaagagaac
     6661 ttcaaaacaa taagagacaa tataaaagaa ggagaagcag gataatactt ttatgtaggt
     6721 atctgtataa ttaaatacat cttcattggc tcccaaaaat tctgtaatat gatgtgaatt
     6781 tataaaagat aaaatcgcaa tgcatagggc cacacaagaa attgtgatgg tattcattga
     6841 gaatacttca tgcatttttc tttcctcatt tgcgccacga tagatcgaca tgacagtact
     6901 tgatccaatc gcaaataaga ctccaatgga gaagatggtt gtgacataag gacttgccac
     6961 gtttatggaa gctaatgccg tatcgccttc aaagtgggca acaaatatac cgtctactaa
     7021 agtatataga ttaaagataa ccatcgaaca tatggatggt atgacaaact taagatattg
     7081 tttcaacata aaaatcacca attatagtgt aaaccttggt ataatactaa ggtcaagggt
     7141 gatggtatga aaaaatatta taaaattaat gaaatcgcta aattatacaa tattaaaaca
     7201 gactctttac gatactatga agaagtagga ttaatttgtc ctatgcgctc aaaatcaaat
     7261 tatcgattat atacactgaa tgatatttat atcttaaata ttattcgaga ttgtttaaaa
     7321 ttagggtatg atacatcaca aatcaaggaa tatttagata atcgaagcgt aaataatacg
     7381 attgcgtttt taaaagaaga agagaagttg attcaaagac agattcaaga attgaacacg
     7441 aaactacttt cgattcaaac tcgaatcata gatttagatc gtaccaaaca aattgatttt
     7501 aatacatgca aaattgaagc atatcccaaa agatattgtc ggtacttaaa agaaaaaatt
     7561 gatcaagatg aaaagattga ctttttatta acgaaactga gtgaatcgat ggaagaagat
     7621 atttctgtac ttggaaatat ggattcaggc agtgtagtcg aatataaaaa taatgcgtat
     7681 gtgtatacaa gtgtctttat tttaactcaa gaagaaaaac atgactttat tctagatgaa
     7741 ggcatatatt gtacatatac ttattcagga gagtatgatc gtacaaacca gctcttttat
     7801 aaaatgaaag attggattca agaacacaat tataaaatcg aaggaccatt cttagaattt
     7861 ttattgattg atatacatga aacaaaaaaa gtggaggagt atatt
//
LOCUS       DBMDWL010000096         7852 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3421_length_7852_cov_3.523416, whole genome shotgun sequence.
ACCESSION   DBMDWL010000096 DBMDWL010000000
VERSION     DBMDWL010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_7852_cov_3.523416"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(70..1464)
                     /locus_tag="ACZPDN_07380"
     CDS             complement(70..1464)
                     /locus_tag="ACZPDN_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013954.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HIR1298481.1"
                     /translation="MKRKRKVKKTSFKLIIILLILVFVVIPFTILKMTEDGQYYVEDL
                     STSEVQASYKHYIFASLKMDTTDSKYTCIKNEDGKVLRLKSGIVNLKTKDVTQNTEYT
                     TDTKETGYVNGNYGADAQYLGTSFNGKKVHFKISGVQAWTDINNVELYLYNDSHILST
                     YYVYNHSLIHTISTDLFQGNVNSIAIGPAPKFMKEDTIYYSYDGHYFYTNYENLVNDN
                     KVNKDPYYNYYQYIPHRTTSYLNNSIYNAYLDQYGVSDESVLYNQADLFFKIQNKYSI
                     NATMMYALALNESGLGLSQYALEYHNLFGHAAIDENPDNANQYSSLAECVKQHAYNFL
                     QQGYLNPNDSRYHGSWFGDKASGINVNYASDPYWGEKAASFYYHLDEGGIDQEKNPIK
                     TIRLSKDLKVYAPNKKDVLYTYKKGNIVSIHILKNEIGYYKISSEAPVKDNDLNVNSK
                     YKNSYVYIKKSDFK"
     gene            1630..2706
                     /gene="prfA"
                     /locus_tag="ACZPDN_07385"
     CDS             1630..2706
                     /gene="prfA"
                     /locus_tag="ACZPDN_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1298482.1"
                     /translation="MAKMDERLEKMVERYREINDLMMQSDVVSDRKTMAKLGREQNEL
                     TPIVETYEEYKKAVEELEEAKVLSKEEDKEIREMAQMELEELEPKIQNYLDRLELLLV
                     PKDPNDSHNAILEIRGAAGGDEGNIFAGDLFRMYSKYAEAKGWRIEVIEMEDSEAGGF
                     SLVSFKVNGEGAYGILKFESGSHRVQRVPKTESQGRIHTSTATVLCMPEVEAEDFDID
                     MNDLEIETMRSSGAGGQHINKTDSAVRIVHKPTGIVVKCQDGRSQHENRATALMTIRA
                     RVYEERQRELDQKNEKERRTKIGTGDRAEKIRTYNYPQNRVTDHRIGYTVNQLDRIMD
                     GRLDDLMAALQLADQQAKIAGEKE"
     gene            2706..3554
                     /gene="prmC"
                     /locus_tag="ACZPDN_07390"
     CDS             2706..3554
                     /gene="prmC"
                     /locus_tag="ACZPDN_07390"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1298483.1"
                     /translation="MSTYHSLINEGKERLYKAGQGEQAAQLFMVELCRQKDINLYISM
                     DEEANEQIQTDYLEGIHLMENGEPLGYVLGYECFYGYDFIVNEDVLIPRPETEELVGL
                     VLSKFDDYFADKDHVNVFDVATGSGAIGITLNLEEPKMDVIASDISKEALVVAQKNNE
                     KLGANVNFICGSMLDPFVDRDLHCDILVCNPPYIPSEEKMEQSVVDYEPHVALFGGND
                     GLKFYRDVFEKAHLVLNEKSFLAFEMGWDQGEALSNLAREYFESATITVHKDMSGKDR
                     MLTIEL"
     gene            3652..4221
                     /locus_tag="ACZPDN_07395"
     CDS             3652..4221
                     /locus_tag="ACZPDN_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HIR1298484.1"
                     /translation="MKYRCLICGHIYDEEKEGVKFADLPADWKCPTCKQPKEKFVPVE
                     DEMTWAAEHVVGVAKDVAEDIKNDLRANFEGECSEVGMYLAMARVAHREGYPEVGLYY
                     EKAAWEEAEHAAKFAELLGEVVTDSTKRNLEMRVEAENGATAGKVDLATRAKKANLDA
                     IHDTVHEMARDEARHGKAFAGLLKRYFGE"
     gene            4317..5183
                     /locus_tag="ACZPDN_07400"
     CDS             4317..5183
                     /locus_tag="ACZPDN_07400"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIR1298485.1"
                     /translation="MIGKNSPCWCGSGKKYKHCHEEWDNTINVLKLQGKIVPSHNLIK
                     SEEDIKWIKKAAKINNAVLDLVGEKICAGMTTEDIDKLVYDYTTSHGGIPACLGYEGF
                     PKSVCTSINNEICHGIPSEKVVLKDGDIINVDCTTIVHKHFADASRMFCIGHVSDEAK
                     RLVEVTKECLEIGKEAVRPWGYIGDIGAAIQEHAHKNGYSVVVDFCGHGVGNAFHEDP
                     YVHHVGIRNTGMVIAPGMVFTIEPMINQGTRDLYIDKDNGWTSYTKDGKLSAQWENTI
                     LVTEKGIEVLAW"
     gene            5232..6569
                     /locus_tag="ACZPDN_07405"
     CDS             5232..6569
                     /locus_tag="ACZPDN_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298486.1"
                     /translation="MQKSMIEGNTLKLILNFSIPLLLGNLFQQFYNVVDAAIVGQTLG
                     SYALAAVGASSSVQFLILGFCMGICQGFAIMVSQSYGAKRLDEMQTYIYAGAVWTAIT
                     AFVVSILVVVFCADILHILQVNHEIFKDAYAYLVVIFAGIPFTLLYNYLSAILRAIGD
                     SKTPFFFLAFSAVLNIGLDFFCILILKWGCAGAAIATIFAQAVSGILCLLLIVFKVNV
                     LHIPKSSRYVDKKHSVKLLAMGVPMGLQFSITAIGSMVMQSANNALGTIYVSGFTAGM
                     RIKSLMMCPFDALGAAVSTFLSQNYGACKLERIYDGYHKGVMVSILYGAFACVVMVFF
                     GRILSMMFVSANESQVLNASALYLRRMGYFYPILGILITTRMSVQGLGYSARAMLAGF
                     VEMIARCIVALCFVPIFKYNAITWADQTAWVAATLVLIPLWISTIRMVQKKMC"
     gene            6704..6829
                     /locus_tag="ACZPDN_07410"
     CDS             6704..6829
                     /locus_tag="ACZPDN_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298487.1"
                     /translation="MSSWDKLLKRFLELDHDIRFEEIVKVMKEYGYEIKQSVIHL"
     gene            6929..7303
                     /locus_tag="ACZPDN_07415"
     CDS             6929..7303
                     /locus_tag="ACZPDN_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1298488.1"
                     /translation="MNQELNYYMNLWYRMEIVEDKDEGGYVLSYPDLPGCMTCSDSLD
                     DLLELAKDARETWILGMLEDEKEIPLPSDLQSYSGQFKLRIPKSLHKSLSMHAKEEGI
                     SMNQYCLYLLAKNDAEYKKERR"
     gene            complement(7313..>7852)
                     /locus_tag="ACZPDN_07420"
     CDS             complement(7313..>7852)
                     /locus_tag="ACZPDN_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298489.1"
                     /translation="VIIDGIKERSFYSPLFGIAIIFYLFKEDLGFEDISLWLLLLATW
                     LLAWGLTSIIHPKKRHIEIEDIEYSTNSIINITSSFGAKVQYIQTNDLQYVTIDSKFS
                     GLKLYFDQATIQNQATIEIDGNFTGIELYVPKEWNVQSHVRAFFGGVDEKGQPSSSGY
                     PTLNITGRVHFGGVEIHYI"
BASE COUNT         2644 a         1116 c         1552 g         2540 t
ORIGIN      
        1 ctctctccta aaaataataa taaacaaaag aaaccatttt aagacgccaa cacttaaaat
       61 ggttttcttt tatttgaaat ccgatttctt gatatataca tagctatttt tatatttaga
      121 atttacattc aaatcattat ctttgacagg agcttcactt gatattttat agtatccaat
      181 ttcattcttt aatatatgaa ttgatacaat atttcccttt ttatatgtgt aaagaacatc
      241 ttttttattt ggtgcatata cctttaaatc cttagatagt cgtattgtct taataggatt
      301 cttttcttgg tcaatcccac cttcatctaa gtgatagtag aaactagctg ctttttcacc
      361 ccaatatgga tcggatgcat agttgacatt tataccactt gccttatcac caaaccacga
      421 accatgatat cttgaatcat ttggatttaa gtatccttgt tgtaaaaaat tataggcatg
      481 ttgttttaca cattcggcta atgaactata ttgattcgca ttatctggat tttcatcaat
      541 tgccgcatgc ccaaataaat tatgatattc aagcgcatac tggctaagtc caagaccact
      601 ctcatttaac gctagggcgt acatcatagt cgcattgatt gaatatttgt tttgaatttt
      661 aaagaataga tctgcctgat tatatagaac agactcatca ctaacaccat attgatctag
      721 atatgcatta tatatagaat tatttaaata ggaagtagtt ctatgaggaa tatattgata
      781 ataattatag taaggatctt tattaacttt attatcatta actaggtttt catagttcgt
      841 ataaaaatag tgaccatcat atgaataata gattgtatct tccttcatga atttaggagc
      901 aggaccaatc gcaatagaat tcacatttcc ttgaaaaaga tctgtagaaa ttgtatgaat
      961 aagggaatgg ttataaacat aatatgtgga taaaatatgg gagtcgttat ataagtataa
     1021 ttcaacatta ttaatatccg tccaagcttg aaccccgctg attttaaagt gaaccttctt
     1081 tccattaaag cttgttccta gatattgagc atctgcacca taatttccgt tcacataccc
     1141 tgtttcttta gtatctgtag tgtattctgt gttttgggtt acatccttcg tctttaaatt
     1201 cacgattcct gatttaagtc gtaatacttt accatcttca ttttttatac atgtatattt
     1261 agagtctgtt gtatccattt ttaaggatgc aaaaatataa tgtttataag aagcttgcac
     1321 ttcacttgtg cttaaatctt cgacatagta ttgtccgtct tctgtcattt ttaaaatggt
     1381 aaatgggata acgacaaata ctagaatcaa tagaataata ataagcttaa atgatgtctt
     1441 tttaactttt ctttttcttt tcatatctca tattatactc ttatctttaa aaattgcgta
     1501 ataggacaat tttcatcgac aataagtgtt ctttatatta aaatagatgg ggtatatcgc
     1561 taaaataaag cgttttttaa gtggtaaaac actcaagata taaaaattat agagaaagga
     1621 tgtatattta tggcaaaaat ggatgagcgt ttagaaaaaa tggttgaacg ttatcgtgaa
     1681 atcaatgatt taatgatgca aagtgatgtt gtatcagatc gtaagacaat ggcaaaattg
     1741 ggtcgtgaac aaaatgaatt gacacctatt gttgaaacat acgaagaata taaaaaggca
     1801 gttgaggaat tagaagaggc taaagtcctt tctaaagaag aggataaaga aattcgtgaa
     1861 atggcacaaa tggaattgga agaattagaa ccaaaaattc aaaattattt agaccgttta
     1921 gaattgcttc tagtaccaaa agatcctaat gattcgcaca atgcgatttt agaaattcga
     1981 ggagcagctg gtggagatga aggaaatatc tttgccggag acttatttag aatgtattca
     2041 aaatatgcag aagcaaaagg atggagaata gaagtcatcg aaatggaaga tagtgaagct
     2101 ggaggattct cattagtttc atttaaagtg aacggggaag gagcatacgg aattttaaaa
     2161 ttcgaatctg gatctcaccg tgtgcaacgt gtaccaaaga cagaatcaca aggtcgtatt
     2221 catacttcta ccgctacagt attgtgtatg ccagaagtag aagctgaaga ttttgatatc
     2281 gatatgaacg atttggaaat tgaaacaatg cgttcaagtg gagcgggagg acagcatatc
     2341 aataaaaccg attcagctgt acgtatcgtc cacaaaccta ctggtattgt agttaagtgt
     2401 caggatggtc gtagtcagca tgaaaaccgt gcaactgcct taatgacaat tcgtgctcgt
     2461 gtatatgaag aaagacaacg tgaattggat caaaagaacg aaaaagaacg tcgtacaaag
     2521 attggtactg gagatcgcgc agaaaagatc cgtacataca actatcctca aaaccgtgta
     2581 acagatcacc gtattggata tacagtcaat cagttagacc gcattatgga tggtcgttta
     2641 gatgatttga tggctgcttt acagttggct gatcaacaag caaaaattgc tggagaaaaa
     2701 gaataatgag tacatatcat tcattgatca atgaaggtaa agaacgtctt tacaaagccg
     2761 gtcaaggcga acaggcagcg caacttttca tggtggaatt atgtcgtcaa aaagatatca
     2821 atttatatat ttctatggat gaagaagcga atgaacaaat tcaaacagac tatctagaag
     2881 gaattcattt gatggaaaat ggagaaccat taggatatgt attgggatat gaatgttttt
     2941 atggatatga ttttatcgtg aatgaagacg ttttgattcc tagaccagaa acagaagagc
     3001 ttgtaggact tgtattatct aaatttgacg attactttgc cgataaggat catgtcaatg
     3061 tatttgatgt ggcaacagga tcgggtgcaa ttggaattac gttaaatctt gaagagccta
     3121 agatggatgt gattgcttca gatatttcaa aagaagcttt agtggttgca caaaagaaca
     3181 atgaaaaact aggcgcaaat gtgaatttca tttgtggaag tatgttagat ccatttgtgg
     3241 atcgtgattt gcattgtgat attttagttt gtaacccgcc atatattcct agcgaagaaa
     3301 agatggaaca aagtgttgta gattatgaac cgcatgtagc tttatttggt ggaaatgatg
     3361 gattgaaatt ctatcgtgac gtatttgaaa aagcacatct tgtattgaat gaaaaatcct
     3421 tccttgcctt tgaaatggga tgggatcaag gtgaagcttt atcaaattta gctcgtgaat
     3481 actttgaatc agcaacgatc actgtacata aagatatgtc aggaaaagat cgtatgttga
     3541 cgattgaatt ataatttaaa aattgttgag caatactttt gaaatattga aatgaatatg
     3601 cgatagtata tgcataagag gaaatattat atttcaagag gaggattatt tatgaaatat
     3661 agatgcttaa tttgtggaca catttatgat gaggaaaaag aaggcgtaaa attcgctgat
     3721 cttcctgcag attggaaatg tccaacttgt aaacaaccaa aagaaaaatt tgttccagta
     3781 gaagacgaaa tgacttgggc tgctgaacac gtggtaggag tcgcaaaaga tgttgctgaa
     3841 gacattaaaa atgatttacg tgctaacttc gaaggtgaat gctcagaagt aggtatgtac
     3901 ttggcaatgg caagagttgc tcacagagaa ggatatccag aagttggatt gtattatgaa
     3961 aaagcagctt gggaagaagc agaacatgct gcaaaatttg ctgaattatt aggcgaagtt
     4021 gttacagatt caactaagag aaatcttgaa atgcgtgtag aagctgaaaa tggtgcgaca
     4081 gcaggtaaag ttgatttagc tactcgtgct aaaaaggcaa acttagatgc aattcatgat
     4141 acagtacacg aaatggctcg tgatgaagct cgtcatggta aggcatttgc tggattatta
     4201 aaacgttatt ttggtgaata atgcatttag aagcttctga atcaatcgga agcttttttt
     4261 cttgttttcg tgtataataa gttgtgttta acgaatatat agaaagaagg atcaagatga
     4321 ttggtaagaa tagtccatgt tggtgtggtt ctggtaaaaa atataaacat tgtcatgaag
     4381 aatgggacaa tacaattaat gtattaaaac tacaaggtaa aatagttcct agtcataatt
     4441 taatcaagtc tgaagaagat ataaaatgga tcaaaaaagc ggctaaaatc aataatgccg
     4501 tacttgattt agttggagaa aaaatctgtg cgggtatgac tacagaagat atcgataaat
     4561 tggtatatga ttatacaaca agtcatggtg gaatccctgc ctgtttaggt tatgaaggat
     4621 tccctaagag tgtttgtaca tctattaata atgaaatttg tcatggtatt ccaagcgaaa
     4681 aagttgtatt aaaagatggc gatattataa atgtagactg cactactatt gtacataaac
     4741 actttgcgga tgcatctaga atgttctgta ttggtcatgt aagtgatgag gcaaaacgct
     4801 tagttgaagt gactaaagaa tgtctagaaa taggtaaaga agccgtacgt ccttggggat
     4861 acattggaga tattggtgct gctattcaag aacatgcaca taaaaatggc tacagtgttg
     4921 ttgttgattt ttgtggacac ggtgttggta atgctttcca tgaagatcca tatgtacatc
     4981 atgtaggaat tcgtaataca ggtatggtta ttgcaccagg catggtcttc actattgagc
     5041 ctatgatcaa tcaaggaacg cgtgatttat acattgataa agataatggt tggacatctt
     5101 atacaaaaga tggaaagtta tcggcgcaat gggaaaacac aattctagtt acagaaaaag
     5161 gaattgaagt attagcttgg taagaagtca ataaaatgac ttcttttttg atggggttaa
     5221 aagaagggaa tatgcaaaag agtatgattg aggggaatac tttaaaactc attttgaatt
     5281 ttagtattcc attattatta ggaaatttat ttcaacaatt ttacaatgtt gtcgatgcgg
     5341 ctattgttgg gcaaacacta gggtcttatg cattggcagc cgtaggtgca agtagtagtg
     5401 tacaattttt gattctagga ttttgtatgg gaatatgtca aggcttcgca attatggttt
     5461 cgcaaagtta tggtgcaaaa agactcgatg aaatgcaaac ttatatctat gcaggagctg
     5521 tatggacagc cattacagct tttgtggtat ccatcttagt cgttgtgttt tgtgcagaca
     5581 ttcttcatat attacaagtt aaccatgaaa tctttaaaga tgcctatgcc tatttagttg
     5641 tcatatttgc agggatacca ttcacattac tctataatta tttatcggct atattaagag
     5701 ctattggaga ttcaaaaacg ccatttttct ttttagcttt ttccgctgtt ttaaatattg
     5761 gccttgattt tttctgtatt cttattttga aatggggatg tgcaggagct gccatcgcaa
     5821 ccatttttgc tcaagctgta agtggtattt tgtgcttact attgattgtg tttaaagtca
     5881 atgttttaca tatacctaaa tcaagtcgct atgttgataa gaaacattcg gttaaattgt
     5941 tggcaatggg cgttcctatg ggcttacaat tttcgattac agccataggt tctatggtca
     6001 tgcaatcagc aaataatgcg ttaggaacca tttatgtttc gggctttacg gccggtatgc
     6061 gtattaaatc gttgatgatg tgtccatttg atgcattggg tgcagctgtt tctacattcc
     6121 tttcccaaaa ttatggagct tgcaagttag aacgaatcta tgatggatat cataaaggcg
     6181 taatggtgag tattttatat ggtgcattcg cctgtgtcgt aatggttttc tttggaagaa
     6241 ttcttagtat gatgtttgtg agcgccaatg aatcacaagt gttaaatgcg agtgcgttat
     6301 atttaagaag aatgggctat ttctatccta ttttaggaat tttaatcaca acaagaatgt
     6361 ccgttcaagg acttggctat tctgctcgag ctatgcttgc aggttttgta gaaatgattg
     6421 cacgatgtat tgtggcatta tgctttgttc ctattttcaa atataatgcg atcacatggg
     6481 cagatcaaac agcatgggtt gcagctacac tggtattgat tccattatgg atcagtacaa
     6541 tacgtatggt tcaaaagaaa atgtgttgaa ttagaagtct aacgacttct ttttttgact
     6601 tttaatatct ttttcacatg catctgattt aattaaggaa agaaatcatc aattaaagat
     6661 gattgacacc aaatatgaca ccaaatttaa tggatgtgaa aagatgtctt cttgggataa
     6721 gctgttaaaa agatttttag aattggatca tgatattcgt tttgaagaga tcgttaaagt
     6781 aatgaaagaa tatgggtatg agattaaaca atcagttatt catttgtaaa accaggatgt
     6841 agtcgtattg tgattctaag acataaacct ataaaaaaag tgtatgtaaa aatgataaaa
     6901 aaagtgatag aagaggagat gaaggaagat gaatcaagaa ctcaactact acatgaatct
     6961 atggtatcgt atggaaattg ttgaagataa agatgaagga gggtatgtct tatcttatcc
     7021 ggatcttcct ggatgtatga catgttcaga ttcattagat gacttgttag aactagccaa
     7081 agacgcaaga gaaacatgga ttttaggtat gttagaagat gaaaaagaga ttccgcttcc
     7141 cagtgatcta caatcgtatt caggacaatt taaattaaga atacccaaaa gtcttcataa
     7201 atccttatcc atgcatgcca aagaagaagg cattagtatg aatcaatatt gtttgtatct
     7261 tctcgcaaaa aatgatgcgg aatataaaaa agaaagacgt tgattgtctt tcttagatat
     7321 aatggatttc aactccacca aaatgaacac gacctgtgat atttaatgta ggatagccac
     7381 tagatgatgg ctgtcctttt tcatcaactc caccaaagaa ggcacgaaca tgactctgta
     7441 cattccattc tttaggtaca tagagttcaa tacctgtaaa atttccatcg atttcaatcg
     7501 tagcttgatt ttgaatggta gcctgatcaa aatagagctt caatcctgaa aactttgaat
     7561 caatggtcac atattgtaga tcattggttt gaatatattg aacttttgca ccaaaactag
     7621 aagtaatatt gatgattgaa tttgtagaat attcaatatc ttctatttca atgtgtcttt
     7681 ttttgggatg aataatgctt gtaagtcccc aagctaatag ccaagtagct aataatagaa
     7741 gccataaaga aatatcttca aaacctaaat cttctttgaa taaatagaaa ataatcgcaa
     7801 tcccaaataa gggactgtag aagcttcttt ctttgattcc atcgataata ac
//
LOCUS       DBMDWL010000097         7742 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3489_length_7742_cov_2.154430, whole genome shotgun sequence.
ACCESSION   DBMDWL010000097 DBMDWL010000000
VERSION     DBMDWL010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3489_length_7742_cov_2.154430"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            141..1286
                     /locus_tag="ACZPDN_07425"
     CDS             141..1286
                     /locus_tag="ACZPDN_07425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1298490.1"
                     /translation="MNYYKTGKFARLANVSERTIRYYDKIGLLKPSFVMENGYRQYSD
                     LDLLKLQKILSLKHLGFSIEEIFPMVMDNTNLKESFELQIDLIEDKIRHLQSLKDALK
                     RASQSSNLSWNMILSLVQLSNEETNIIEQYKNAKNLNDRIRLHEKYSTNKQGWFNWLF
                     EKIDFSKVNRLLELGCGNGKLWQENKIELRNREIFLSDISEGMVEEVRNKLGSDFNCI
                     VADAEKIPFKDAYFDSIIANHVLFYLNDLNQGLKEIRRVLKPNGVLYCSTYGSKHMKE
                     ITEIVQNFDSRINLSNHSLYDVFGLENGESILKQYFTNVQRMDYQDSLEITESKPLID
                     YIMSCHGNQNEILGPRLSEFKEYIEELLVNNGKIIVTKQAGLFVCEK"
     gene            1307..3445
                     /locus_tag="ACZPDN_07430"
     CDS             1307..3445
                     /locus_tag="ACZPDN_07430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1298491.1"
                     /translation="MNKAMEVLKREGISGLAKHASHYIHKKRESRPKSDAFKDVLFIN
                     GCPRDLLPHPPRYRVTHQMEQLKVNGYTCDEVFFENVDLKQVRMYSCFIIFRAPYTEK
                     LDAFIQLAKTWNKPVLYDVDDLVIDTKYTDQIAYVKNMEASQKARYDLDVMKNQKLLK
                     LCDGCITTTLALKKELEKYNPNVWINRNTSSIEMVTLSQEIKKTSKDTVDIGYFSGSI
                     THNEDFEMIKPALIDVLNAYDYVRLHLVGEIDLPQDLKMYASKIEVHPFMDYKKLPEL
                     ISKMDINLAPLTSNIFNEAKSENKWVEASLVNTCTIASNLGAFKEMIEDHKTGILCSN
                     IVEWKKKLTCLIENKKLRETLAGNAYAYCLKHCVTLYTGSNIVSILQENRKRIICMGF
                     AKLEISGGVMVALRHAAYLQDAGYQVMLLSFYDTCTEFTFMNHSFPVLPFKNEKLDAK
                     IDCGVATMWPTVKMLDDVRCIENKKYLVQGYEVDFYDFKDPRKVEASVTYSYPFDCIT
                     VSKWCKEWLKSEFNKDAKWIYNGIDIEQYKNHKREFNGKIRILIEGDSSSKYKNVDEA
                     FTITNKLDSSKYEIWYMSYNAKPKDWYRVDKFLHRVPYEEVQKVYADCDILLKTSVFE
                     SFSYPPLEMMATGGYVVALLNDGNKEYLEDGKNCLIYPNGDIDKAVHCIERIVSDQIL
                     RDVLYTNGVETAKSRDWNLIKESIIRVYCA"
     gene            3513..4391
                     /gene="rfbA"
                     /locus_tag="ACZPDN_07435"
     CDS             3513..4391
                     /gene="rfbA"
                     /locus_tag="ACZPDN_07435"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR1298492.1"
                     /translation="MKGIILAGGSGTRLYPLTKVTSKQLLPIYDKPMIYYPMSILMQA
                     GIREILIISTPDDTPRFQELLGDGHQFGIELSYKVQPSPDGLAQAFILGEEFIDDDSC
                     AMILGDNIFHGHGLTKRLRAAAENEVGATVFGYYVEDPERFGVVEFDSEGKAISLEEK
                     PACPKSNYAVTGLYFYDNKVVEYAKNLKPSARGELEITDLNRIYLENGELNVTLLGDG
                     FTWLDTGTHESLVDATNFVKTIEEHQHRKIACLEEIAYDNGWIGDEELASTYEIYKKN
                     QYGAYLKKIMDRKYIK"
     gene            4405..4977
                     /gene="rfbC"
                     /locus_tag="ACZPDN_07440"
     CDS             4405..4977
                     /gene="rfbC"
                     /locus_tag="ACZPDN_07440"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR1298493.1"
                     /translation="MKVTETKIPGVLIIDTDVFGDHRGYFTETYSKPKYEKMGITVDF
                     VQDNMSFSAQKGTLRGLHWQNPPYAQSKLVSCTKGTVIDVAVDIRKGSPKYGQWVSVE
                     LSAENHRQFFIPQGFAHGFLTLTDDVEFRYKVDNVYNKESEGGMRYDDPTCNVDWGSL
                     LNGIEPVLSEKDQVGPTLEESNNQFVYKGE"
     gene            4982..>5793
                     /gene="rfbB"
                     /locus_tag="ACZPDN_07445"
     CDS             4982..>5793
                     /gene="rfbB"
                     /locus_tag="ACZPDN_07445"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1298494.1"
                     /translation="MKILVTGGAGFIGGNFVHYMVNKYPEDMIVNLDKLTYAGNLETC
                     KPVEDKPNYKFIKGDIADREFIMDLFEKEKFDVVVNFAAESHVDRSIEDPEIFVKTNV
                     MGTTTLLDACVKYGIQRYHQVSTDEVYGDLPLDRPDLFFTENTPLHTSSPYSSAKASA
                     DLFVLAYHRTYGLPVTISRCSNNYGPYHFPEKLIPLIISRALNDETIPVYGTGENVRD
                     WLHVYDHCVAIDLIVRKGRVGEVYNVGGHNERTNLEVVKTVLHALNKPESLIT"
     assembly_gap    5794..5803
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5804..6027
                     /locus_tag="ACZPDN_07450"
     CDS             <5804..6027
                     /locus_tag="ACZPDN_07450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR1298495.1"
                     /translation="YVTDRKGHDMRYAIDPTKLETELGWKPKYTFDTGIPMTIQWYLD
                     NKEWWENIISGEYQNYFEKMYVEKGRAKE"
     gene            6066..6980
                     /gene="rfbD"
                     /locus_tag="ACZPDN_07455"
     CDS             6066..6980
                     /gene="rfbD"
                     /locus_tag="ACZPDN_07455"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR1298496.1"
                     /translation="MKVFVTGVAGQLGHDVMNELASRGYVGVGTDLAESYSGIQDGTY
                     VTTAEYVSLDITNSEAVMNTIQSVKPDVVVHCAAWTAVDLAEDEDKQAKVKAINVDGT
                     QNIADACKEIDAKMVYISTDYVFDGQGTDPWQPDCKDYKPLNVYGQTKLGGELAVSNT
                     LSKYFIVRIAWVFGKNGNNFIKTMLNVGKKFDTLKVVNDQIGTPTYTYDLARLLVDMI
                     ETDKYGYYHATNEGGYISWYDFACEIFKQAGYTNTVIPVTTKEYGLSKAARPFNSRLD
                     KSKLVENGFKPLPTWQDALKRYSQEIEF"
     gene            7007..>7742
                     /locus_tag="ACZPDN_07460"
     CDS             7007..>7742
                     /locus_tag="ACZPDN_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298497.1"
                     /translation="MNKLLARIRNKKKFTLFILIFLMFFMVSSIVTYKSFISPFSTND
                     NVIHSSKPDSEKEKWIRLDGSYVNQTLTYVTPYLTGVGVHLHDVDPGSENTLHFLLKK
                     DNETIYSWDLKEKDLNDGMCILRMDTNPVDMANVDFEFYSDGITNIKFGIVQNDREKF
                     AKINKCKINNVQTNYSIAYDVVSGNCAEIKGLFLLVVLLFVLSIFLFYMFICLETKIS
                     TIAFSMIFILGLIYSLVLPQFSVPDEW"
BASE COUNT         2605 a          917 c         1618 g         2592 t
ORIGIN      
        1 gagttggatt tctatatgta ttattatggt gaagactgca ttgataacat tattaatttg
       61 attgatacaa atcatagatt aaatcaagaa ttaagtgaaa ttaaggagtc taagatatat
      121 agattaacaa aaaataagat atgaattatt ataaaacagg taaattcgca cgtcttgcga
      181 atgtgagtga aagaacaatt cgttattatg acaagatagg gttattaaaa ccctcttttg
      241 ttatggaaaa tggatatcgt caatatagtg atttggattt attgaagtta cagaagatct
      301 tatctttaaa acatttgggg tttagtattg aggaaatctt tccaatggtt atggacaata
      361 ccaatcttaa ggagagcttt gaattgcaga ttgatttgat tgaggataag attcgtcatc
      421 tacaatcttt aaaagatgct ttaaaaaggg cgagtcaatc ttcaaatctt tcttggaata
      481 tgattttatc tttagttcaa ttgtcgaatg aagaaacgaa tattattgag caatataaga
      541 atgcgaagaa cttaaatgat cgtattcgtc tgcatgaaaa gtattctaca aataaacagg
      601 gatggtttaa ttggttgttt gaaaagattg atttttctaa agtaaatcgc ttattagagc
      661 taggttgtgg caatggtaag ttgtggcaag agaataagat tgaattaaga aatcgagaaa
      721 tattcttatc ggatattagt gaaggtatgg tggaagaggt tcgtaataaa ttgggttctg
      781 attttaactg tattgtggcg gatgctgaaa agataccttt taaagatgct tattttgatt
      841 ccatcattgc caatcatgtt ttgttttatt tgaatgattt aaatcaggga ttaaaagaga
      901 tccgtcgagt attaaaacca aatggagtgt tatattgtag tacatatggt agtaagcata
      961 tgaaagaaat tacagaaatt gtacaaaact ttgattctag aataaatctt tcgaatcata
     1021 gtttgtatga tgtatttggt ttagaaaatg gtgaaagtat tttaaaacaa tatttcacga
     1081 atgttcaaag aatggattat caagattcat tagaaattac agaatccaag cctttgattg
     1141 attatattat gtcttgtcat ggcaatcaaa atgaaatatt ggggccaaga ctatctgagt
     1201 ttaaagaata tattgaagag ttgttagtaa ataatggtaa aataattgta actaaacaag
     1261 ctggcttgtt tgtgtgtgag aagtaggagg tgttattttg gagttagtga ataaagcaat
     1321 ggaggtatta aaacgagaag gaatttccgg tttagcaaaa catgcctcgc attatattca
     1381 taagaaaaga gaatcaagac ctaaaagtga tgcatttaag gatgtcttgt ttataaatgg
     1441 gtgtcctaga gatttgttgc cacatccacc tcgttatcgt gtaacacatc aaatggaaca
     1501 gctaaaagtg aatggctata cttgtgatga agttttcttt gaaaatgtag atctaaaaca
     1561 agtgcgtatg tattcttgtt tcattatttt tagagcgcct tatacagaaa aattggatgc
     1621 atttattcaa ttagctaaaa catggaataa acctgtactt tatgatgtgg atgatttagt
     1681 cattgataca aaatatacag atcagattgc gtatgttaag aatatggagg cttctcaaaa
     1741 agctcgttat gatttagatg ttatgaagaa tcaaaagtta ttaaagcttt gcgatggatg
     1801 tatcacaacg actttggcct tgaagaagga gcttgaaaaa tacaatccaa atgtatggat
     1861 caatcgaaat acgtcttcta ttgaaatggt aactttatca caagaaatta agaaaacatc
     1921 aaaagatact gtggatattg gttactttag tggtagtatt acgcacaatg aagattttga
     1981 aatgattaag ccagccttga ttgatgtgtt gaatgcatat gattatgtgc gtttgcatct
     2041 tgttggagaa atcgatttac ctcaagattt aaagatgtat gcttctaaga ttgaagtaca
     2101 tccttttatg gattataaaa aacttcctga attgatttct aagatggata ttaatttagc
     2161 gcctttaact tcaaacattt ttaatgaagc aaaatcagag aataaatggg tagaggctag
     2221 tttagtaaat acttgtacga ttgcttcaaa tctaggtgct tttaaagaga tgatagaaga
     2281 tcataaaact ggaatacttt gttctaatat agttgagtgg aagaaaaaat taacttgttt
     2341 gattgagaat aagaaattaa gagaaacttt agctggtaat gcgtatgcat attgtttaaa
     2401 gcattgtgtt actttatata caggttcaaa tattgtttct attctacaag agaatcgtaa
     2461 acgaattatt tgtatgggct ttgcaaaact tgaaatttct ggtggagtta tggtggcttt
     2521 gcgtcatgcc gcttatttac aggatgcagg ttatcaagtg atgttattat ctttctatga
     2581 tacttgtaca gaatttactt ttatgaatca ttcatttccg gtcttgccat ttaaaaatga
     2641 aaagttggat gcgaaaattg attgtggtgt agcgacaatg tggcctacgg ttaagatgtt
     2701 ggatgatgtt cgttgtattg aaaataaaaa gtatttggtg caggggtatg aggttgattt
     2761 ttatgatttt aaagatcctc gtaaagtaga agctagtgtg acttattcat atccatttga
     2821 ttgcattact gtctccaaat ggtgtaaaga atggttgaag tccgaattta acaaagatgc
     2881 caagtggatt tataacggaa ttgatattga acagtataaa aatcataaac gagagttcaa
     2941 tggaaaaatt cgaattttga ttgagggaga ttcttcaagt aagtataaaa atgtggatga
     3001 ggctttcacg attacaaata aattggattc ttccaaatat gaaatatggt acatgtctta
     3061 taatgccaag ccgaaggatt ggtatcgtgt tgataaattc ttgcatagag taccttatga
     3121 agaggtgcaa aaggtatatg cagattgtga tattttgtta aaaacttctg tttttgaaag
     3181 ctttagttat ccaccacttg aaatgatggc cactggtggt tatgtggtgg ccttattaaa
     3241 tgatggcaat aaagaatatt tagaggatgg aaaaaattgt ttgatttatc caaatggaga
     3301 tattgacaag gctgttcatt gtattgaaag aattgtttca gatcaaattt tgcgtgatgt
     3361 tttatataca aatggtgtag aaactgcgaa atcaagagat tggaatttga ttaaagaatc
     3421 aataattcgt gtatattgcg cttgacccta acgtaacgtt agggtttatt cttttattgt
     3481 gatataatga tgttaattat ggaggactcg acatgaaagg tattatttta gctggtggta
     3541 gtggtactcg tttgtatcct ttgacaaagg ttacaagtaa gcaattgctt cctatttatg
     3601 acaagccaat gatttattat ccaatgtcta ttttaatgca ggcaggaatt cgtgagatct
     3661 tgattatttc aactccggat gatacacctc gttttcaaga attactaggg gatggtcatc
     3721 aatttggaat tgagctttct tataaagttc agccaagtcc tgatggactt gcacaagcct
     3781 ttatcttggg cgaagaattt attgatgatg atagttgtgc aatgattctg ggcgataata
     3841 ttttccatgg tcatggtttg acgaagcgtc ttcgtgcggc tgctgaaaat gaagtgggag
     3901 ctacagtatt tggttattat gtagaagatc ctgaacgttt tggtgttgtt gaatttgatt
     3961 ctgaaggcaa agcgattagt ttagaggaaa agccagcttg tcctaagtca aattatgcgg
     4021 taacaggact ttatttctat gataataaag tggtagaata tgcgaaaaac ttaaagccaa
     4081 gtgctcgtgg agaacttgaa attacagatt tgaaccgtat ttatttagag aatggcgaat
     4141 tgaatgtaac tttacttggt gatggattta cttggttaga tacgggtaca catgaatctt
     4201 tagtggatgc cactaatttt gtgaagacaa ttgaagagca ccaacatcgt aagattgctt
     4261 gtcttgaaga gattgcgtat gacaatggat ggattggtga tgaagaattg gcttctacgt
     4321 atgaaattta taagaagaat cagtatggtg cttatttaaa gaagatcatg gatcgtaagt
     4381 atattaagta atgggggtat agtaatgaag gtaactgaaa caaagattcc tggcgtattg
     4441 attattgata cagatgtatt tggagatcat cgtgggtatt ttacagaaac gtattcaaag
     4501 cctaaatatg aaaagatggg tattacggtt gattttgtgc aggacaatat gtcttttagt
     4561 gcacagaagg gtacgcttcg tggattgcac tggcaaaatc cgccttatgc acaaagtaaa
     4621 ttggtttctt gtacaaaggg aactgtcatt gatgttgcgg ttgatattcg caaaggtagt
     4681 cctaaatatg gtcaatgggt gagtgtggaa ctgagtgcag aaaatcatag acaattcttt
     4741 attccacaag gatttgcgca tggtttctta actttaacgg atgatgttga atttagatat
     4801 aaagtcgata atgtctacaa taaagagtct gagggtggaa tgcgttatga tgatccgact
     4861 tgtaatgtag attggggatc attattaaat ggtattgagc ctgtcttaag tgaaaaggat
     4921 caggtaggtc caactttaga agagtcaaat aatcaatttg tatataaagg agaataaaaa
     4981 tatgaaaatt ttagtaacgg gtggtgctgg tttcattggt ggaaactttg tacattatat
     5041 ggtcaataaa tatcctgagg atatgattgt aaacttagat aaattaacat atgcaggtaa
     5101 cttagagact tgtaagcctg tagaggataa gcctaactac aagtttatca agggtgatat
     5161 tgcagatcgt gaatttatca tggatttgtt tgaaaaagaa aaatttgatg tcgttgttaa
     5221 ctttgcggct gaaagtcatg tggatcgtag tattgaagat cctgaaatct tcgtaaaaac
     5281 aaacgtaatg ggtacgacta ctttattaga tgcttgtgtt aagtatggta ttcaaagata
     5341 tcaccaagta tctacagacg aagtatatgg tgatttgcca ttagaccgtc ctgacttgtt
     5401 ctttactgaa aacacacctt tacatacttc aagtccttat tcaagtgcaa aggcaagtgc
     5461 tgatttattt gtacttgcat atcatagaac atatggttta ccagtaacaa ttagtcgttg
     5521 ttcaaataac tatggtcctt atcatttccc ggaaaaatta attccattaa ttatttctcg
     5581 tgccttaaat gatgaaacaa ttccagtata tggaactgga gaaaatgtgc gtgactggtt
     5641 acatgtatat gatcactgtg tcgcaattga tttaattgtt cgtaagggac gtgttggtga
     5701 agtctataat gtaggtggtc acaatgaaag aacaaactta gaagttgtaa agactgtact
     5761 tcatgcatta aacaaaccag aaagcttgat tacnnnnnnn nnncgtatgt aacagatcgt
     5821 aaaggtcatg acatgcgtta tgcgattgat ccaactaagt tggaaacaga acttggatgg
     5881 aaaccaaaat atacatttga tacaggtatt ccaatgacaa tccaatggta tttagacaac
     5941 aaggaatggt gggaaaacat cattagtggt gaataccaaa actattttga aaagatgtat
     6001 gtagaaaaag gaagagctaa ggaataatct ttagcttttc ttattaaatg aaaggaagat
     6061 attctatgaa ggtttttgtg acaggtgttg caggtcaatt aggtcatgat gtcatgaatg
     6121 aattggcttc tagaggatat gttggagtag gtacagattt agctgaaagc tatagtggaa
     6181 ttcaagatgg tacgtatgta acaacagctg aatatgtatc tttagatatc actaattcag
     6241 aggctgtaat gaatacaatc cagtctgtaa agccagatgt tgttgtgcat tgtgcagcat
     6301 ggactgcagt tgatttagcg gaagatgaag ataaacaagc aaaggtaaaa gctatcaatg
     6361 tggatggtac acaaaatatt gcggatgctt gtaaggaaat agatgcaaag atggtctata
     6421 tttctacaga ttatgtattt gatggtcaag gaacagatcc ttggcaacca gattgtaagg
     6481 attataaacc attaaatgtg tatggacaaa caaagcttgg tggagaacta gctgtttcaa
     6541 atactttatc taagtatttc attgttcgta ttgcgtgggt atttggaaag aatggaaata
     6601 actttattaa gacgatgtta aatgtaggaa agaaatttga tacattaaaa gtcgtaaatg
     6661 atcaaattgg tacaccaacc tatacttatg atttagcccg tttattagtt gatatgattg
     6721 aaacggataa atatggttat tatcatgcga caaatgaggg tggctatatc agttggtatg
     6781 attttgcgtg tgaaatcttt aaacaagcag gttatacaaa tactgtaatt ccagtaacaa
     6841 cgaaagagta tggtttatct aaggcagctc gtccttttaa ttctcgtttg gataaatcaa
     6901 aattggttga aaatggattt aaacctcttc caacttggca ggatgcttta aagagatatt
     6961 cacaagaaat tgaattttaa ttaaattaga aataggagta gtttgtatga ataaattatt
     7021 ggctagaatc cgaaataaaa aaaaatttac acttttcata ttgatatttc taatgttttt
     7081 tatggtatct tctatagtca cttataaatc atttatttct ccattttcga ctaatgataa
     7141 tgtgattcat tcttcaaaac ctgattctga gaaagaaaaa tggattcgtt tagatggaag
     7201 ttatgttaat caaacgttaa cgtatgtaac tccatattta acgggtgttg gtgttcatct
     7261 acatgatgta gatccaggct cagaaaatac attgcatttc ttacttaaga aagataatga
     7321 gacgatttat tcttgggatt taaaagaaaa agatttaaat gatggaatgt gtattttaag
     7381 aatggataca aatcctgtgg atatggcaaa tgttgatttt gaattttatt cggatggcat
     7441 aacaaatata aaatttggaa tcgtgcaaaa tgatcgtgaa aaatttgcga aaatcaataa
     7501 atgtaaaata aacaacgtgc aaacgaatta cagtattgcc tatgatgtag taagtggtaa
     7561 ttgcgcagaa atcaaaggtc tttttttgtt agtagtttta ttgtttgtgt tgagtatttt
     7621 cttgttttat atgtttattt gtttagaaac aaaaatatct acaattgcat tttcaatgat
     7681 ttttatttta gggcttattt actcattggt attgccacag tttagtgtac ctgatgaatg
     7741 gt
//
LOCUS       DBMDWL010000098         7681 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3536_length_7681_cov_1.896702, whole genome shotgun sequence.
ACCESSION   DBMDWL010000098 DBMDWL010000000
VERSION     DBMDWL010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3536_length_7681_cov_1.896702"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            172..1905
                     /locus_tag="ACZPDN_07465"
     CDS             172..1905
                     /locus_tag="ACZPDN_07465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR1298498.1"
                     /translation="MNTHFNYSDTLSKDEIKEAKAYLDEQEQVDFMNHTQIRKDLNQL
                     LKNGSYQVILYKTKSNKPDDKVIDSVLAFCGRAPLKNAQKYSVFIKSSSKTSCIEWYP
                     SFKNYYASYRIFDCILSLIITILFYLLLKNLKPLRSKKNVFYSLSSRMMFSFLCATLL
                     VLTSFSFLYLNRSAVFEFLMDTFYQSEDYSSYVKKLEKQLQNFDLTKENKKAINHILK
                     ENALNHAYVELYDQDGIYFTDKSPLSSNNFLLLYSFDDLDVLEAPLYYDYPIHFKNQS
                     VYLYITSFPMIPFQIPYIILSLLISFSFYLIILQSFIRKRIHSIQSLQEDVFTLAIGE
                     WNHEIKVSDKDEIGRLAQDLNQMRIAFLQTMDNEQQAHVANKELISSLSHDLRTPLTT
                     LKGYLEIMNLKRDDIEFRDQYLQKCLAKVEEITYLSNKMFEYSLVFSTEYNSDLSNIP
                     IQRVINTLVDHIQYLREMDLHILYEPLQTSLSMDANFAMIQRILNNVFSNIQKYCDPW
                     KDIVIQATIEEDRLKMSFTNSINRHLDKVESNGIGLKSVKKMIELHHGTFYQDETNDL
                     FTIILTFPIHQ"
     gene            2039..2491
                     /locus_tag="ACZPDN_07470"
     CDS             2039..2491
                     /locus_tag="ACZPDN_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIR1298499.1"
                     /translation="MPKTKFENIVFTLLMSFLMVYAMICYNIALNMGGMSNQVFLLAF
                     HELVIMWPVAFILEYFFVDKLAHHFAFKVVTPKDRPFFITLAISFYIICIMCPIMSLI
                     ATILFKTPGVQIISVWFMTILFNFPMAFFWQICYCGPTIRWLFGKLFN"
     gene            2559..3176
                     /locus_tag="ACZPDN_07475"
     CDS             2559..3176
                     /locus_tag="ACZPDN_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR1298500.1"
                     /translation="MNKELIEELKAQDFNVVAVSKTRTKEEIDEVAKMGLTTFGENRV
                     QEFIDKYDPKYTWHIIGHLQTNKVKYIVGKVDVIESLDSLKLAEEIEKQANKHGIIQK
                     VLVELKISEDPNKTGYPFKDAKNFISQLQEFKHIQIKGIMCVASKTEDQELVESEFEQ
                     MHTLYLELKEQYPEIDTLSMGMSQDYKLAVKHGSNTVRIGHAIFE"
     gene            complement(3385..4656)
                     /locus_tag="ACZPDN_07480"
     CDS             complement(3385..4656)
                     /locus_tag="ACZPDN_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR1298501.1"
                     /translation="MDSDLWIQILELVVLLALSGFFSSAETAMVSVSKIRLRTLEEEG
                     NKKAALALKILENQSKMLSAILIGNNLVNTSAASIASVIAYSFGGAAVSIATFIITFL
                     ILVFGEITPKTWATINADKLALAYAPIITFLMKILTPVIWFVNLFSSGILKLMGIQNS
                     NKNISMTESELRTIVDVSHEEGVIEEDEKDMINKVFDLGDAKAKDVMVPRVHVVMAEI
                     DSTYKDLLEIFREEKFTRIPIYQDKIDNIVGVVNMKDLLMYDDFSHFDMQKVLRKPKF
                     TYENKKVSELLIEMKQSTFNLAIVMDEYGEFSGIVTLEDIIEEIVGDVQDEYDAHEEE
                     NIIKLNNQTYDVKGYLSLHDLNDYLDLDLDSEDFDSVGGLVIDALGRLPQTNDETTLP
                     NGIRIKVLKVEKNRIESVRLILPDTQNEKDA"
     gene            complement(4695..5594)
                     /locus_tag="ACZPDN_07485"
     CDS             complement(4695..5594)
                     /locus_tag="ACZPDN_07485"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1298502.1"
                     /translation="MKDVFIVNPKSGKNIQYELIKEIKEHFQGKRIIIEKTKGPEHAT
                     FIAKKYALSNEPVHLFVCGGDGTLHEVINGCAEKENVTISVIPIGTGNDFVKYFEDLK
                     REDFLNLANYSEPEYIDCDLIKVNGEYSINTVSFGFDVEVAKQVNELKKKMPTEGIIP
                     YALSALISLRKPICKEYQIQIDTKKLPKDKYGFLVFANGKYYGGGFKPCPDANIDDGW
                     MDVCLISNVKRHQIIKLAKKYQEGNHLQYTNLVSMYQAKTVHLDTENELIYANLDGEV
                     KGFKNPTIEIVEKAIRLALPRIS"
     gene            complement(5612..6301)
                     /locus_tag="ACZPDN_07490"
     CDS             complement(5612..6301)
                     /locus_tag="ACZPDN_07490"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1298503.1"
                     /translation="MMKTNERSELLLGKKALETLNDKTVLVVGVGGVGSFCVEALART
                     GIGHLILIDKDCVEPSNINRQLVATLDTVDQIKVNVLKERIRTLNPNCIVDTFAFFYD
                     QTKDDAIFSQPIDFVVDCIDSIQSKKDLMQACIDRNIPFLSSMGMARRKDPTKLVVTE
                     IEKTSYDPMAKQIRQWKRKNRIRNKIWVVASTEIPIPVESGQPLPSAIFVPASAGLLL
                     ASTCVDRLIEV"
     gene            complement(6285..7199)
                     /gene="miaA"
                     /locus_tag="ACZPDN_07495"
     CDS             complement(6285..7199)
                     /gene="miaA"
                     /locus_tag="ACZPDN_07495"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR1298504.1"
                     /translation="MDRQKVLAIVGPTGIGKTSLSVWLAKQLNGEIISCDSMQVYKKM
                     DVGTAKVTKEEMQDIHHELIDVQNYDEPYNVKVFQEKCRTAIEDVVKRGKFPILCGGT
                     GLYLKAALYDYEFQEEQEDTAYTTYLNSKTNEELVVMLEEKDAKALEKIHPNNRKRLI
                     RALQICHSGTSKSEQEDKQQHIPLYDVYFLGLDVKREILHDRINKRVDIMFENGLVKE
                     VEELFSNPKTWEYTSFQGIGYKEFKDYFLKEKSIEEVKTAIKTHSRQYAKRQYTWFKN
                     QMPVHWFEAQNRDEIYSAVKEWLDDEDK"
     gene            complement(7192..>7681)
                     /locus_tag="ACZPDN_07500"
     CDS             complement(7192..>7681)
                     /locus_tag="ACZPDN_07500"
                     /inference="COORDINATES: protein motif:HMM:NF020260.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1298505.1"
                     /translation="AAQERYHYEQLNEKLLVQCTNKQPLMVPIQLDVSSNVLAQYMTI
                     NEKTAFFGIEFEPFGTDQLILREIPLWFHDVDQKQFLQDLLDYFVENQDVDMAKLRKH
                     MIATMACHSSIRFNRPLSMQEMEQVILDLQKCKQPYHCPHGRPTVIVMSDGELRKEFE
                     RG"
BASE COUNT         2432 a         1308 c         1075 g         2866 t
ORIGIN      
        1 acaaataaat tacaagcata aaaaaatgag catcactatt tgtgacactc atttaacaag
       61 aactgtcaga tatttgtcct tgacttttga accagtttat atctttccct tcttgttttt
      121 ttattgagtt ttttcgcaat atattctcaa agatttgtcg tatacgatta tttgaataca
      181 cattttaatt attcggatac tttgtctaaa gacgagatca aagaggcgaa ggcatatttg
      241 gatgaacaag aacaagtgga ttttatgaac cacactcaaa ttcgtaaaga tttaaatcaa
      301 ttattaaaaa atggaagtta tcaagtcatt ttatataaaa ctaaatcaaa taaacccgat
      361 gataaagtca tcgattctgt acttgcgttt tgtggtcgtg ctcctttaaa aaatgcacaa
      421 aagtatagtg tttttataaa aagttcaagt aaaacaagtt gtattgagtg gtatccatct
      481 tttaaaaatt attatgcttc ttatcgtatt tttgattgta ttttaagtct tattatcaca
      541 attctttttt atttactttt aaaaaatctt aaaccactcc ggtcaaagaa aaacgtcttc
      601 tattccttat ccagtcgtat gatgttctct tttctttgtg caacattgtt ggtattaact
      661 tctttttcat ttctatattt aaatcgtagt gctgtctttg agtttttaat ggatactttt
      721 tatcaatcgg aagattattc ttcttatgta aagaaattag aaaaacaact ccaaaatttt
      781 gatttgacga aagaaaataa aaaagccatc aatcatatat taaaagaaaa tgcactcaat
      841 catgcgtatg tggaattata tgatcaggat ggaatctatt ttacagacaa atctccatta
      901 agcagcaata attttttgtt gttatatagt tttgatgatt tggatgtctt ggaggcccca
      961 ttgtattatg attatccaat acattttaaa aaccaatccg tttatttata tatcacttca
     1021 ttcccaatga ttccattcca aattccttat attattcttt cgcttttgat ttctttctct
     1081 ttttatttaa ttatcctaca aagttttatc cgtaaacgaa ttcattctat tcaaagttta
     1141 caggaagatg tatttacttt agcaatcggt gaatggaatc acgaaatcaa agtaagtgat
     1201 aaagatgaga ttgggcgttt agcccaggat ttgaatcaaa tgcgcattgc ctttctacaa
     1261 acaatggaca atgaacaaca ggcgcatgtt gcgaataaag aattaatttc ttctttatcc
     1321 catgatttaa ggactccact aacaacttta aagggatacc ttgaaatcat gaatttaaaa
     1381 agggatgata ttgaatttcg tgatcaatat cttcaaaagt gtctagctaa agttgaagaa
     1441 atcacatatc tttccaataa aatgtttgag tattctcttg tcttttctac agaatacaat
     1501 tcggatttat cgaatattcc cattcaaaga gtcatcaata cattggtaga tcatattcag
     1561 tatttaagag aaatggatct acacatttta tatgagcctc tacaaacttc tctatctatg
     1621 gatgcgaatt ttgcgatgat tcaaagaata ttaaataatg tattttcaaa tattcaaaag
     1681 tattgtgatc cttggaaaga tattgtgatt caagcaacga ttgaagaaga tcgattaaaa
     1741 atgagtttca cgaattcaat caatcgtcat ttagataaag tcgaaagtaa tgggattggt
     1801 ttgaagagtg ttaagaaaat gatagaactc catcacggta ctttctatca agacgaaaca
     1861 aatgatttat ttactattat attaactttc cctattcatc agtaagagtg attcttactg
     1921 atttttttat tacaaaatta gaaaatttat ggtattatta ctagcgttca ctttttggta
     1981 gatattggaa acgtctttga ttgaacaaca caaaaagtca tttaggagga gaaaatatat
     2041 gccaaaaaca aaatttgaga atattgtatt cactttatta atgtcttttt taatggttta
     2101 tgccatgatt tgttacaaca tcgcactcaa tatgggtggt atgtcaaatc aagtcttttt
     2161 gttagctttt catgaattgg ttatcatgtg gccagtagct tttattttag aatatttctt
     2221 tgttgataaa cttgctcatc attttgcctt taaagttgta acaccaaagg atcgaccttt
     2281 ctttattaca cttgcgattt ctttctacat tatttgtatt atgtgtccaa tcatgagttt
     2341 gattgcgacc attctattta aaactccagg tgtacaaatt atttctgtat ggtttatgac
     2401 catcttgttt aattttccaa tggctttctt ttggcagatt tgttattgtg gaccaactat
     2461 aagatggttg tttggaaagc tatttaacta gaagcaacgt attgcttctt ttttttcttc
     2521 gtctataatt aaatttgtat tgaaaggact gttttaatat gaataaagaa ttgattgaag
     2581 aactaaaagc acaggatttt aatgtggtag ctgtatctaa aacgcgtaca aaggaagaaa
     2641 ttgatgaagt cgccaaaatg gggctaacta cttttggaga aaatcgtgta caggaattta
     2701 tagataagta tgatccaaaa tatacttggc acattattgg gcatctacag acaaataaag
     2761 tgaagtatat tgttggaaag gttgatgtga ttgaaagctt ggattcattg aaacttgctg
     2821 aagaaattga aaaacaagcc aataaacatg gcattattca aaaggtattg gtagaactaa
     2881 agatttcgga agatccaaac aagacgggat atccttttaa ggatgctaaa aactttattt
     2941 ctcaactaca agaatttaaa catattcaaa tcaaaggcat aatgtgtgtt gcttcaaaaa
     3001 cagaggatca agaattagtt gagtctgagt ttgaacaaat gcatacttta tatttagaat
     3061 taaaagaaca atatcctgaa atagatactt tatctatggg tatgtctcaa gattacaagt
     3121 tagctgttaa acatgggtca aatacagttc gtattggtca tgctattttt gaataatatc
     3181 aaaagaaaaa accgatatat tgattattta aaatcattta tatcggttct tattttatag
     3241 tttagtaaac accagacagg cagcacctag taatccacta tcttcattta atgtcgattt
     3301 tcttaccttt actaaaggtg ctttttcttt taaaagcttg aatacctacg acataataca
     3361 atttactttg catgcttgtc ggtactaagc atccttttca ttttgtgtat ccggtaaaat
     3421 cagtctaact gattctatac ggtttttttc tacttttaag actttaatgc ggattccatt
     3481 tggtaaagtt gtttcatcgt ttgtctgtgg caaacgtcct agtgcatcaa tgacaagtcc
     3541 tccaacagaa tcaaagtctt cactatctaa gtctaaatct aaataatcgt ttaaatcatg
     3601 taaacttaaa tagcctttta catcatatgt ttgattattt aatttaataa tattttcttc
     3661 ttcatgtgca tcgtattcgt cttgtacatc tccaacaatt tcttcaataa tgtcttctaa
     3721 cgtaacgata cctgagaatt ctccatattc atccatgact atcgcaagat taaatgtgga
     3781 ttgtttcatt tcaatcaata attcagatac ttttttattt tcatacgtaa atttaggttt
     3841 acgtagaact ttctgcatat caaaatgtga aaaatcatca tacatcaata agtctttcat
     3901 atttacaacc ccgacaatat tatcaatctt atcttgataa attggaatac gcgtgaattt
     3961 ttcttctcta aatatctcca ataaatcttt atatgtactg tcgatttctg ccatcacaac
     4021 atgaacacga ggaaccatta catcttttgc cttggcatcc cccaaatcaa atactttatt
     4081 gatcatatct ttttcatctt cttcaatgac accttcttca tgtgaaacat ctacaattgt
     4141 acgcaattca ctttcagtca ttgatatatt tttatttgaa ttttgtattc ccattaattt
     4201 tagtatgcct gaactaaata aattcacaaa ccaaataaca ggcgttaata ttttcatcaa
     4261 aaaagtaatt ataggtgcat acgcaagcgc aagtttatct gcattgatcg tagcccatgt
     4321 ttttggtgtg atttcaccga atactaaaat taaaaaagta ataataaatg tagctatact
     4381 aacagctgct ccaccaaaag aatatgcgat cacagaagca atacttgcag ctgaagtatt
     4441 cactaagtta ttaccaatca atatagcact tagcattttc gactgatttt ccaatatttt
     4501 taaggctaat gcagcctttt tatttccttc ttcttctaat gttctcaaac gaattttact
     4561 gactgaaacc attgccgttt cagctgaaga aaaaaagcct gataatgcta ataaaactac
     4621 taactctagg atttgtatcc ataaatccga atccattttt cacactccct atatttttga
     4681 atattgtatc atactcacga tattctagga agggcaagtc gaattgcctt ctctacaatt
     4741 tcgattgttg gatttttaaa tcctttgact tctccatcta gatttgcata aataagctca
     4801 ttttccgtat ctaaatgaac tgtttttgcc tgatacatag agactagatt tgtgtattga
     4861 aggtgatttc cttcttggta tttttttgcc aattttatga tttgatgtct tttcacattt
     4921 gaaatcaagc aaacatccat ccatccgtca tcaatattgg catctggaca tggtttaaag
     4981 cctccaccat agtattttcc attcgcaaat actaaaaatc catatttatc tttcggtaat
     5041 ttttttgtat cgatctgaat ttgatattct ttacatatcg gttttcttaa actgatcaaa
     5101 gcggataatg cgtatggaat aataccttct gtaggcattt ttttctttag ttcatttact
     5161 tgttttgcga cttcaacatc aaaaccaaag gaaacggtat ttattgagta ttctccattt
     5221 acttttatta agtcacaatc tatatattct ggttctgaat agtttgccaa atttaaaaag
     5281 tcttctcttt ttaaatcttc aaaatatttc acaaaatcat ttcctgttcc aatcggaatc
     5341 acagaaatgg tcacattttc tttttctgca catccattaa taacttcatg caaagttcca
     5401 tctccaccac aaacaaataa atgtacaggt tcattggata gagcatattt ttttgcgata
     5461 aatgtcgcat gttcaggacc tttggttttt tcgataatga ttctttttcc ttgaaaatgt
     5521 tctttaattt ccttaatcaa ttcatattga atgttctttc cagatttagg atttacaata
     5581 aatacgtcct tcatatactc ttccttccgt tttatacttc aatcaatcga tctacacagg
     5641 tactcgcaag aagtagtcct gcacttgcag gaacaaaaat tgcacttggt aatggttgtc
     5701 ctgattctac tggtatagga atttctgtag atgctacaac ccatatttta tttcgaatac
     5761 gattttttcg tttccattga cgaatttgtt tagccattgg atcataactt gtcttttcta
     5821 tttctgtaac aacaagtttt gtaggatctt tgcgcctagc cattcccata cttgataaga
     5881 atggtatatt tcgatctata caggcttgca ttaaatcctt tttactttga atggaatcaa
     5941 tgcagtctac cacaaaatca ataggttgtg aaaaaatcgc atcatctttt gtttgatcat
     6001 agaaaaatgc aaatgtatcc acaatacaat ttggatttag tgtacgaatt ctttctttca
     6061 atacattgac tttaatttga tctactgtat ctaaagtcgc aaccaactgt cggttgatat
     6121 tacttggctc tacgcaatct ttgtctataa gaattaaatg accgattcca gttcttgcta
     6181 aggcttctac acaaaaagat ccaactccac ccacaccaac aacgagaacc gttttatcat
     6241 ttagtgtttc tagagctttt tttccaagca acaattcact tctttcattt gtcttcatca
     6301 tccaaccatt cctttactgc actatatatt tcatccctat tctgtgcttc aaaccaatgt
     6361 acaggcattt gatttttaaa ccatgtatat tgtcttttcg catattgtct agaatgtgtt
     6421 tttatagctg tttttacttc ctctatactt ttttctttta agaaataatc tttaaattct
     6481 ttatatccaa taccttgaaa acttgtatat tcccatgttt tgggatttga gaataattct
     6541 tctacttctt tgaccaaacc attttcaaac ataatatcca ctcttttatt gattcgatca
     6601 tgcaaaattt ctcgtttcac atctaaacct aaaaagtata catcatacaa tggaatatgt
     6661 tgttgtttgt cttcttgttc tgattttgaa gtaccactat gacaaatttg tagagcacga
     6721 attaaacgtt tacgattgtt aggatgaatt ttttctagag cttttgcatc tttttcttct
     6781 aacatcacaa ccaattcttc atttgtttta gaatttaaat aggttgtata agctgtatct
     6841 tcttgttctt cttgaaattc atagtcatat aacgctgcct ttaaataaag tcctgttcca
     6901 ccgcataaaa taggaaactt tcctcttttt acaacatctt cgatagctgt acgacatttt
     6961 tcttgaaaaa cctttacgtt ataaggttca tcataatttt ggacatcaat caattcgtga
     7021 tgaatatcct gcatttcttc tttagtaact tttgcggtac caacatccat ttttttatat
     7081 acttgcatac tatcgcaaga tattatttct ccattcaatt gttttgccaa ccatacagaa
     7141 aggcttgttt ttccaattcc ggttggtcct acaattgcta aaactttttg tctatccacg
     7201 ttcaaattcc tttctcaact caccatcact catcacaatg actgtaggtc ttccatgagg
     7261 acagtgatat ggttgtttac acttttgtaa atctaaaatg acttgctcca tttcctgcat
     7321 agataatggt cgattaaaac gaattgaact atggcaagcc atagtcgcaa tcatatgttt
     7381 tctaagtttt gccatatcta catcttgatt ttccacaaaa taatctaata aatcttgtaa
     7441 gaattgtttt tggtccacat catgaaacca tagtggaatt tcacgaagaa tcaattgatc
     7501 tgtgccaaat ggttcaaatt caatcccaaa aaatgctgtt ttttcattga ttgtcatata
     7561 ttgagctaaa acattacttg atacatccaa ttgaatagga accattaatg gttgtttatt
     7621 tgtacattga accaatagtt tctcattcaa ttgttcataa tgatatcttt cttgggcagc
     7681 a
//
LOCUS       DBMDWL010000099         7601 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3590_length_7601_cov_3.747733, whole genome shotgun sequence.
ACCESSION   DBMDWL010000099 DBMDWL010000000
VERSION     DBMDWL010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7601)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3590_length_7601_cov_3.747733"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(51..452)
                     /gene="rpsI"
                     /locus_tag="ACZPDN_07505"
     CDS             complement(51..452)
                     /gene="rpsI"
                     /locus_tag="ACZPDN_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR1298506.1"
                     /translation="MKKSNTVQYHGVGRRKKSVARVFLRPGTGNIVVNGKTLEEYLPL
                     ETLRMVVKSPLEITNTADQFDVIINVQGGGYTGQSGAMRHGITRALMEASEDYRPVLK
                     AAGFVTRDPRAKERKKYGLKAARRAPQFSKR"
     gene            complement(466..903)
                     /gene="rplM"
                     /locus_tag="ACZPDN_07510"
     CDS             complement(466..903)
                     /gene="rplM"
                     /locus_tag="ACZPDN_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR1298507.1"
                     /translation="MRQTTMAKPADVTRTWYVVDGNGQTLGRLASTCAHVLKGKHKPT
                     YTPNVDCGDYVIVINADKVEMTGNKLNTEKYYNHSGYFGGMRVRTAKEMKEKYPVEWV
                     ERAIKGMLPHTKLGDVQRGHVFVYAGSEHPHAAQKPVELKVRG"
     gene            complement(1021..1845)
                     /locus_tag="ACZPDN_07515"
     CDS             complement(1021..1845)
                     /locus_tag="ACZPDN_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR1298508.1"
                     /translation="MRQTNFHTHTSRCHHAYGEDEEMVRAAIQNGFEVLGISDHTCWK
                     YDSDFVAHMRMKLDEFADYKDSVLYLKDRYKKQIEIRFGLEAEYFPKYMDWLLDFCIE
                     NEIDYLIFGNHYYGTDEDHIYLGGAKGEYIKGYFDTCVEGMKTGMYAYLAHPELILRD
                     TGGFITPEIEEGFHRICKTSQDYNVPLEFNVLGMQHNMRMGYEEYPHTKFWQIAAQYN
                     VKAILGMDAHRIGDLDQKLYNLALEKLSKFDVEIIDDVPSIDYKKIKAEKIKVWQK"
     gene            complement(1832..2575)
                     /gene="truA"
                     /locus_tag="ACZPDN_07520"
     CDS             complement(1832..2575)
                     /gene="truA"
                     /locus_tag="ACZPDN_07520"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1298509.1"
                     /translation="MIVKALVSYDGFNYAGWQKQENALGIQQVIEEALYKITKENVDV
                     VASGRTDAGVHAFGQVFHFETNKNIPAIQYERALNALLPKDIRILKTEEMPDDFHARF
                     SAVGKRYDYYCSYDIKNPFNYKYRNLLTKKLDIEAMKNASKVFLGSHDFTSFASSKID
                     PRKPRVKTISNIEIIEEGMDVHFIFEGTGFLRYQVRMMTGTLIAVGQHKIGVDDVQRM
                     LDAKDKSACRYNASSCGLYLVEVKYNETN"
     gene            complement(2572..3369)
                     /locus_tag="ACZPDN_07525"
     CDS             complement(2572..3369)
                     /locus_tag="ACZPDN_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1298510.1"
                     /translation="MNNVALGQYMPLDSVVHKMDPRSKIMIMLFLMVAIFIPAGVLGY
                     VIIGIFILLSLYLSKLNIKYALRTMKPMLWMMAFLLVINLLVIKTGTPLFSINGFTIY
                     SDAVNQTLYIVVRLMLMIIITTVLTATTKPLDLTLGIEKLLKPFEKIGVPAHIIAMMI
                     SIALRFIPTLIEETQRIMNAQASRGVDLENGSIKEKIMAILSLIVPLFVSAFDRADQL
                     ANAMEARGYDPSRKRTRYKVLKMQTIDYASMILSVVVLCACIGIWVL"
     gene            complement(3362..4222)
                     /locus_tag="ACZPDN_07530"
     CDS             complement(3362..4222)
                     /locus_tag="ACZPDN_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1298511.1"
                     /translation="MSIEIKKLEHTYNENTPFSHAALKGINLSIPEGKVTAIIGQTGS
                     GKSTLVQHLNGLLIPTAGTLDICGFHIQPLLKIKDVKQLRKEVGLVFQFPEYQLFEET
                     IGKDIAFGPKNFGTSEEDASQLVKKILPVVGLDESYLDRSPFDLSGGQKRRVAIAGIL
                     VLNPKVLVLDEPTAGLDPQGAQEMMTLFMNLNKKEGKTVLLVTHDMEHVMNYCDHVIV
                     LSHGEVTQEADVKEFFKHPEYLQEIGINPPSIVRLKMQLEENGFEMDPDIMDMNSLVD
                     SIEKQVKKHE"
     gene            complement(4207..5025)
                     /locus_tag="ACZPDN_07535"
     CDS             complement(4207..5025)
                     /locus_tag="ACZPDN_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1298512.1"
                     /translation="MEAIEVSHLTFSYDGKNDILKDVSFSIPKGSYTTIIGHNGSGKS
                     TMAKLIIGLLEAKKGKIQILGKELNEESVYELRSHVGIVFQNPDNQFIGSTVADDIAF
                     GLENHCVEQEKMQGIIEDVASRVGMTDFLSAEPTKLSGGQKQRVAIAGILAIEPDIII
                     FDESTSMLDPQGKASINAQIRKLHEEKNITILSITHDMEEVAQSENVIVLENGEIAMQ
                     GTPMEIFKQEDKLAKMQLDIPFALKISKELKKRKIFKENVCRLDEVVEKLCRLK"
     gene            complement(5092..5544)
                     /locus_tag="ACZPDN_07540"
     CDS             complement(5092..5544)
                     /locus_tag="ACZPDN_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298513.1"
                     /translation="MKLFKFLLGAATITVGACAVAGKMRRDKQRQEELDEFLCPDIDE
                     PIEKEIKSNESPLKHMEADIMGFKDAQEDILPVTLTYSFDSKDSAKQFQTEIAKNGLT
                     SSYDDEDKIVDVIYHDGVNESDLKILSDTLVNACTNASAEYKGFHFNK"
     gene            complement(5547..6116)
                     /locus_tag="ACZPDN_07545"
     CDS             complement(5547..6116)
                     /locus_tag="ACZPDN_07545"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR1298514.1"
                     /translation="MSDLEQYAKEHQVPIMMDDGLSFLLEKLQENQCLSFLELGTAIA
                     RTSIEVAKLDPRMRVVTIERNSEMIVQAKKNIEESGLSNQITLIEGDALEVNVDGMFD
                     CIFIDAAKAQYQRFFEKYCMHLNENGIIVSDNMNFHGLVQHPELTQNRNTRQLVRKIH
                     DYHTYLANLSDYETAFYDYGDGVAVTRRK"
     gene            complement(6158..6520)
                     /gene="rplQ"
                     /locus_tag="ACZPDN_07550"
     CDS             complement(6158..6520)
                     /gene="rplQ"
                     /locus_tag="ACZPDN_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR1298515.1"
                     /translation="MWNRKLGRKADHRKALLRNMATSLIENGQIETTEMKAKELSAVM
                     DSLVTLAKRGDLHARRQAAAYVRNVEVDEEGTTALQKLFNEIGPAYADRNGGYTRVVK
                     TRIRRGDAAPMAIVQFVK"
     gene            complement(6535..7485)
                     /locus_tag="ACZPDN_07555"
     CDS             complement(6535..7485)
                     /locus_tag="ACZPDN_07555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR1298516.1"
                     /translation="MQEFERASLHKDFVNEENNTGSYIFEPLERGFGITIGNTICRTL
                     LTSVPGTRIVGFKVSGFDGKATMVDGILEDIYRISLNLKAVQLYNDGEGFQTLHISKK
                     GPATIQAADIICPEAVEVVDPEQVICTLEKGASLEMEIYAVTGTGYKSSIENKVSYDF
                     GEDVVVLDATFTPIRKADYLSEPARVGLDKKYDKVHINVETDGTITPLQAITMAAKVC
                     MENLDEVLTVSDLELEESFMVQKPQVEDVKKVNTMMIEDLDLSVRSYNCLKRAGIQTV
                     DELTQKTEDEMMHVKNLGKKSLKEVKDKMYQLGLFFKSYE"
     gene            complement(7502..>7601)
                     /gene="rpsK"
                     /locus_tag="ACZPDN_07560"
     CDS             complement(7502..>7601)
                     /gene="rpsK"
                     /locus_tag="ACZPDN_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR1298517.1"
                     /translation="RALQVAGLEITMINDVTPIPHNGCRPPKRPRG"
BASE COUNT         2351 a         1627 c         1033 g         2590 t
ORIGIN      
        1 cttgattttc cggggttttt gaaccatttt gatacggttt gaacagcaga ttatctcttg
       61 ctgaactgag gtgcacgacg tgctgccttg agaccgtatt ttttacgttc tttagcacga
      121 gggtcacgag taacgaatcc tgcagctttt aatactggac ggtagtcttc actagcttcc
      181 attaaagcac gagtaattcc gtgacgcata gcaccagatt gaccagtgta tcctccacct
      241 tgtacgttga taatcacatc aaattgatca gctgtgttag tgatttctaa tggtgattta
      301 acaaccatac gtaaagtttc caatggaagg tattcttcca aagtttttcc gttgactacg
      361 atattacctg tacctggacg taaaaagaca cgagcaacag attttttacg acgtccaacg
      421 ccatgatatt gaacagtgtt agattttttc atattctatt cctccttatc ctcttacctt
      481 caattcaaca ggtttttgag cagcatgtgg atgttcgcta ccagcgtata cgaatacgtg
      541 tccacgttgt acatcaccta atttagtgtg aggtaacata cctttgattg ctctttcaac
      601 ccattcaaca gggtatttct ctttcatttc tttagctgta cgcacacgca tacctccaaa
      661 gtatccagag tggttgtaat acttttcagt attcaacttg tttccagtca tttcgacctt
      721 gtcagcattg atcacaatta cataatcacc acagtcaaca tttggtgtgt atgtaggttt
      781 gtgtttacct tttaatacat gagcacaagt tgaagccaaa cgtcctaaag tttgtccgtt
      841 tccatcaact acataccatg tacgtgttac atcggcaggt tttgccatag tagtttgacg
      901 catatctaat ttcctccttg atttgagcgt ttaccggggc tcaaaaggcc agagtttagc
      961 catctaatat gatatgcaat atccacataa aagtcaatga aaaaaagaaa aagtttagct
     1021 ttatttttgc caaactttaa tcttttctgc ttttattttt ttataatcaa ttgaaggtac
     1081 atcatcaatg atttctacat caaatttaga tagtttttcc aaagccaaat tatataattt
     1141 ttgatccaaa tcacctattc tatgcgcatc catacctaga attgccttca cattatattg
     1201 agcagcgatt tgccaaaact tagtgtgtgg atactcctca taccccatac gcatattgtg
     1261 ttgcataccc aatacgttaa attctaatgg aacattataa tcttgactgg tcttacaaat
     1321 acggtgaaat ccctcttcaa tttcaggcgt gataaaaccc cctgtatctc ttaaaatcaa
     1381 ttcaggatga gctaagtatg catacatacc tgttttcata ccttcgacac aagtatcgaa
     1441 atatccttta atatattctc ctttggcccc acctaaataa atatgatcct catctgtacc
     1501 ataataatga ttgccaaaaa tcaaataatc aatttcattt tcaatgcaga aatctaacaa
     1561 ccaatccata tacttaggaa agtattctgc ttctaagcca aaacgaattt caatctgctt
     1621 cttatatcga tctttcaaat ataaaacact atctttataa tctgcaaatt catccagttt
     1681 catacgcata tgtgccacaa aatcggaatc atatttccaa catgtatgat ctgaaatacc
     1741 taatacttca aagccatttt gaatggcagc acgaaccatt tcctcatcct caccatacgc
     1801 atgatgacaa cgtgaagtat gtgtatgaaa attagtttgt ctcattatat tttacctcca
     1861 ctaaatataa accacagcta gacgcattat aacgacatgc cgacttatct tttgcatcta
     1921 acattctttg gacatcatca acacctatct tatgttgacc aaccgcaatt aaagtacccg
     1981 tcatcattcg aacttgatat cttaaaaaac ctgtaccctc aaagataaaa tgcacatcca
     2041 taccctcttc aataatttca atatttgaaa tcgttttgac tctaggtttt ctcggatcga
     2101 ttttacttga ggcaaaactc gtaaaatcat gagatcccaa gaatactttt gatgcatttt
     2161 tcatcgcctc aatatccaat tttttagtta ataaattacg atacttataa ttaaaaggat
     2221 tctttatatc gtacgaacaa taataatcat aacgtttgcc tacagcacta aaacgtgcat
     2281 gaaaatcatc gggcatttct tccgttttta aaatacgaat atctttggga agcaatgcat
     2341 ttaatgctct ttcatattga atagccggaa tatttttatt tgtttcaaaa tgaaaaactt
     2401 gaccaaacgc atgaacacca gcatccgttc ttccactcgc aacaacatcc acattctctt
     2461 ttgtgatctt atacaaagct tcttcaatca cttgttgaat gcctaaggca ttctcttgtt
     2521 tttgccagcc tgcataatta aaaccatcat agctgacaag tgcttttaca atcataatac
     2581 ccaaatacca atacaagcac aaagaacgac aacacttaaa atcattgaag cataatcgat
     2641 agtctgcatc tttaacactt tgtaacgtgt tctttttcta gaaggatcat accctctagc
     2701 ttccatcgca ttcgcaagtt ggtctgcacg atcaaatgca gaaacaaata gtggaacaat
     2761 caacgataaa atggccataa ttttttcttt gatactccca ttttccaaat ccacaccacg
     2821 actagcctgc gcattcataa tacgctgtgt ttcttcaatt agtgttggaa taaaacgcaa
     2881 cgcaatactg atcatcatcg caataatgtg agctggtaca ccaatttttt caaaaggctt
     2941 caacaatttt tcaattccca aagtcaaatc caaaggcttt gttgttgctg ttaatacagt
     3001 tgtgataata atcatcaaca ttaaacgtac cacaatatac aaagtttgat ttacggcatc
     3061 actataaatt gtaaatccat taatcgaaaa caaaggtgtt cctgttttaa taaccaatag
     3121 gttaataacc aacaagaaag ccatcatcca taacatgggt ttcattgttc ttaaggcata
     3181 tttaatattt aattttgata aatacaaact caataaaata aaaataccta taatcacata
     3241 tccaaggact cctgcaggaa taaatatcgc aaccattaaa aacaacatga tcataatctt
     3301 agatcgagga tccatcttat gaaccacaga atctaatggc atatattgtc ctaacgcaac
     3361 attattcatg tttcttcacc tgcttttcta ttgaatccac aagtgaattc atatccataa
     3421 tatctggatc catctcaaat ccattttctt ctaattgcat ctttaaacgc acaatactag
     3481 gtggattgat accaatctct tgtaaatatt caggatgctt aaaaaattcc ttcacatccg
     3541 cttcctgcgt tacttcacca tgactcaata cgatcacatg atcacaataa ttcatgacat
     3601 gctccatatc gtgagttact aaaagaaccg tttttccttc ttttttattt agattcataa
     3661 acaaagtcat catttcttga gcaccttgag gatcaagccc tgctgttggc tcatctaaaa
     3721 ctaagacttt tggatttaat actaaaatcc ccgcaatcgc aacacgacgt ttttgtcctc
     3781 cactcaaatc aaaaggagaa cgatccaaat aactttcatc caaacctaca acaggcaata
     3841 ttttctttac aagttgggaa gcatcttctt cacttgttcc aaagtttttt ggtccaaacg
     3901 caatatcctt accaatcgtt tcttcaaaca attgatattc aggaaactgg aatacaagcc
     3961 caacttcctt tcttaattgt ttaacatcct taattttcaa caaaggctga atatggaaac
     4021 cacaaatatc caaagtaccc gctgttggaa tcaacaaacc atttaaatgc tgaactaaag
     4081 tagatttacc agaaccagtc tgcccaataa tagctgtaac ttttccttct ggaattgaaa
     4141 gattgatacc ctttaatgca gcatgtgaaa aaggtgtatt ttcattatat gtgtgttcta
     4201 acttctttat ttcaatcgac ataacttttc aaccacctca tctaaacgac atacattttc
     4261 tttgaagatt ttacgcttct ttaattcctt cgaaattttt aacgcaaaag gaatatctaa
     4321 ctgcatttta gccaatttat cctcttgttt aaaaatttcc attggcgttc cctgcatcgc
     4381 aatctcacca ttttctaata ctatgacatt ttcagattga gctacctctt ccatatcatg
     4441 ggtaatggat aaaattgtga tattcttttc ttcatgtaac ttgcgaatct gcgcattgat
     4501 actcgcctta ccttgaggat ctaacatgct tgtggattca tcaaaaataa tgatatcagg
     4561 ttcaatggcc aaaatacctg caatcgcaac acgttgtttt tgtccaccag ataatttagt
     4621 cggctctgca cttaaaaaat ccgtcatacc aacacgactt gcgacatctt caataatacc
     4681 ttgcatcttt tcttgttcta cacaatgatt ttctaaacca aaggcaatat catctgccac
     4741 tgtagaacca ataaattgat tatccggatt ttgaaatact attccaacat gacttctcaa
     4801 ttcataaaca ctttcttcat ttagttcttt acctaaaatc tgaattttcc cctttttcgc
     4861 ctccaacaaa ccaatgatta gttttgccat tgtactctta ccagaaccat tatgtccaat
     4921 aatcgttgta tagcttcctt ttgggattga aaaagaaaca tcttttaata tatcattttt
     4981 cccatcatag gaaaaagtta aatgtgaaac ttcaattgct tccatcacat atcctccacc
     5041 ataaacggaa ctattatacc atattaaaat ggcttgagca ccctcaagcc actatttatt
     5101 aaaatgaaat cctttatatt cagcactcgc atttgtgcaa gcatttacaa gtgtatcgga
     5161 taagattttt aaatcactct cgtttacacc atcatgataa attacatcta caattttatc
     5221 ttcatcatca taagaagaag ttaagccatt ctttgcaatt tctgtctgga attgttttgc
     5281 gctatctttt gaatcaaaac tataagttaa agttactgga agaatatctt cttgtgcatc
     5341 tttaaatccc atgatatctg cttccatatg ttttaatgga ctttcattcg atttgatttc
     5401 tttttcgata ggttcatcaa tgtctggaca taagaattca tctaattctt cctgtctttg
     5461 tttgtcacga cgcattttac ctgcaactgc acaagctcca actgtaatgg ttgcagcacc
     5521 taataaaaac ttaaaaagct tcatagttat ttcctccttg ttacggcaac tccatctcca
     5581 taatcataaa atgcggtttc ataatctgaa agatttgcca aatacgtatg ataatcatgg
     5641 atcttacgaa ctaattggcg tgtattacga ttttgagtca attccggatg ttggacaagc
     5701 ccatgaaaat tcatattatc tgatacaatt ataccattct cattcaaatg catacaatat
     5761 ttctcaaaaa aacgttgata ttgagcctta gctgcatcta taaaaatgca gtcaaacata
     5821 ccatccacat tcacttcaag agcatcgccc tcaatcaatg taatttgatt ggataatcca
     5881 ctttcttcaa tgttcttttt agcctgaaca atcatttccg aatttctttc aattgtgaca
     5941 actctcattc tagggtctaa cttagcaact tcaatcgatg tacgagcaat cgcagttcct
     6001 aactctaaaa atgatagaca ttgattttct tgaagttttt ctaataaaaa ggaaagtcca
     6061 tcatccatca tgataggcac ttgatgttct tttgcgtatt gttctaaatc actcatattt
     6121 ttttaagctc cttaaaagaa aagtgaagta gtaactacta cttcacgaat tgtacaatag
     6181 ccattggagc tgcatctcca cgacggatac gtgtcttaac tacacgagtg tatccaccat
     6241 tacgatctgc atatgctggt ccaatttcat tgaataattt ttgaagagct gttgttccct
     6301 cttcatctac ttctacatta cgaacataag ccgctgcttg tctacgtgca tgtaaatctc
     6361 cacgttttgc taatgttact aatgaatcca ttacagcgct taattctttt gctttcattt
     6421 ctgttgtttc gatttgtccg ttttcaatca aagatgttgc catgttacgc aacaatgctt
     6481 tacgatgatc ggctttacgt cccaatttac gattccacat gattcttact cctcttattc
     6541 atatgatttg aagaacaatc ccaattggta cattttgtcc ttcacttctt tgagtgattt
     6601 cttacccaaa ttcttaacat gcatcatttc atcttctgtt ttttgtgtaa gttcatcgac
     6661 ggtttgaatt cctgcacgtt ttaaacaatt gtaagaacga acagataaat ccaaatcttc
     6721 aatcatcatt gtatttactt tcttaacatc ctcaacttga ggtttttgaa ccatgaaact
     6781 ttcttctaat tctaagtcag aaacagttaa aacttcatct aagttttcca tacaaacctt
     6841 tgcagccatt gtaatagcct gtaaaggtgt aattgttcca tctgtttcta cattgatatg
     6901 tactttgtca tattttttat ccaatccaac acgagcaggt tctgataaat aatctgcttt
     6961 acggattgga gtgaaagttg catctaatac tacgacatct tcaccaaagt cgtatgacac
     7021 tttgttttca attgaactct tatatccagt tccagttact gcataaattt ccatttctaa
     7081 acttgcacct ttttctaaag tacaaatcac ttgttcagga tctacaactt ctacagcttc
     7141 cggacaaata atgtcagccg cctgaattgt tgctggtccc ttctttgaaa tatgcaatgt
     7201 ttggaatcct tctccatcat tgtataattg tacagctttt aaatttaatg aaatacgata
     7261 aatatcctct aaaataccat caaccattgt tgcttttcca tcaaatccag atactttaaa
     7321 tccaacaatg cgtgtgccag gtacacttgt taataatgta cggcaaattg tatttccaat
     7381 cgtaattcca aaacctcttt ctagaggttc aaagatataa gatccagtat tgttctcttc
     7441 attcacaaaa tctttatgaa gtgaagctct ttcaaattcc tgcatcgtta tcctcctttt
     7501 actatccacg aggtcgtttt ggtggacgac atccgttgtg tggaattggt gttacatcat
     7561 tgatcattgt aatttctaaa cctgcaactt gtaatgctct t
//
LOCUS       DBMDWL010000100         7579 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3604_length_7579_cov_2.790853, whole genome shotgun sequence.
ACCESSION   DBMDWL010000100 DBMDWL010000000
VERSION     DBMDWL010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3604_length_7579_cov_2.790853"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /locus_tag="ACZPDN_07565"
     CDS             <1..1795
                     /locus_tag="ACZPDN_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIR1298518.1"
                     /translation="QFQGNISDLQKSIKAICANAFSETIHEDEMEVRIYHLPTHLLEQ
                     MSVDKYEKEEATFVRIDSFEKHELGEKMIQMWEQLLNAYVDLKNKVNSQARFLEIGQD
                     AVRHYYDVLVFEQSYHDARIDAMNSLVMDVLNIVKNAKNIILPVNCAYVLTRMIISTG
                     RNTSLLNQWESNYSKDIQECLQTMSDMMMNESIIADQIIKQIKTATNVSLSNMNKIFL
                     MLNIRMYNKELQGQDTMGIILSHGYSTASSIADAANSLLNHYIFEAIDMPLDTPVEEI
                     TKKLNEYINNNMHLKNIILLVDMGSLEEIGDVISDTMNVGVINNISTSLALNVGMKMI
                     QHHDLETILSESCEENQCHYKILSVAKKEKAIAITNDVSVSVSEKLSHLLQNSLPKKV
                     DIKFIEYDYDCLQKNKHDDPLFEKYDVVLMVKPLSLKLKGVRSVSLEDIMNFKDIDIL
                     NSIFKPYLDEDEIEEFNQNLLKNFSIQSVMENLTILNPQKLLDFVSESVQNLQLVMQK
                     KFQSRTIIGIYIHICFLVERLVTKTAIEIYENLDDFANNHSDFIEQVNQSFDSMLKNY
                     HVKMPISEIAYLYEYIADDEDERKVASDDEF"
     gene            1782..2615
                     /locus_tag="ACZPDN_07570"
     CDS             1782..2615
                     /locus_tag="ACZPDN_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1298519.1"
                     /translation="MMNFKDKLCGMNSHYRFYSFERFFQEMNRLGIKNVEIWTGPMHF
                     YVDYNKNQSVEQLKEYAKKYDVKIIGICPEQTNPKPNNVASPTRQEDVYAYYCRMVDI
                     AKEVNAQHILITTGWAYYDESKEDAWNRSVEMMRKVCDYAKANDILVAIEALQPDESV
                     LVNSVEQLKAYLDAVNHPQLKVCIDFGAMARVNNTIQDYFDAFGKDVIHTHFVDGKPT
                     GHLAWSDGTRDLKQDLLDLEANGYHGYLSLETATSRYYEKPWVAEETTLKAFEKLGKE
                     D"
     gene            2618..3028
                     /locus_tag="ACZPDN_07575"
     CDS             2618..3028
                     /locus_tag="ACZPDN_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1298520.1"
                     /translation="MRHFILASHGTFSRGIYNSVQIILGEQPNTHIITAYVEEGQDVN
                     DLIEESLKDIPDTDPVIVCTDVFGGSVNNEWMKRLSRPNTHLITGMNLGLLMNVFMRQ
                     DDDIENMIRQSVQEAKDGLIYCNDLLSNDNEEEF"
     gene            3040..3510
                     /locus_tag="ACZPDN_07580"
     CDS             3040..3510
                     /locus_tag="ACZPDN_07580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1298521.1"
                     /translation="MIKLCRVDHRLLHGQVAFSWTNAVQADCILVASDEVVNDEMWKT
                     TLKLGKPAGVKLVIKDIENSVKALNSGVTDKYKLLIVVQTIHDAYRLVQEVPAIKSIN
                     LGGAKKQDGYRQISKAIFVGPQDEEDIKAMLDKGVEVEIRQLASESSVPVQSVL"
     gene            3538..4314
                     /locus_tag="ACZPDN_07585"
     CDS             3538..4314
                     /locus_tag="ACZPDN_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008687636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIR1298522.1"
                     /translation="MLLQAILIGLVAALGVLGDQMGSLYINRPIILAPLVGLIVGNLE
                     QSIVIGASLELFFMGAVSVGAYIPPDTIVGGVLATAFALSTGRGIETAVTLAMPIALV
                     SQALGNLCNVFNSIILRWTDDYAEKGNYNGVVFTHWLIGMITVVRRFLLVLFAYYLGG
                     DAVGNLINAIPEFVTNGMAAAAGLLPALGFAMLMRMTINKQNIPYYFLGFILAAYMGV
                     PVLGVAMIGVILVLVKFNFLDKQAATEAEAVQGVSDDDDF"
     gene            4301..5122
                     /locus_tag="ACZPDN_07590"
     CDS             4301..5122
                     /locus_tag="ACZPDN_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1298523.1"
                     /translation="MMTSKITKKDITNLAFNQGSLGMEFSWNYERQMHIAFAMMMNHN
                     LKKIYEGDEEGYRDALVRHVEFFNVTPQLAPFIGGVVTSMEEMKARGEVDGEAIASIK
                     TALMGPLSGIGDSIFVGCLRVIAVGIGISLAQAGNIMGPILYFLLYNIPCFIVRYFGA
                     HFGYNLGFNYLQKMQENGMMDKLLSAASILGVMVVGCMSKDMVWTTLNVELSRIGDEV
                     TTLQSILDGIMPGLIGLGGTWLYYFLLKKKVNPVILIFGTMIIGVIGAYFGVLAG"
     gene            5149..6147
                     /locus_tag="ACZPDN_07595"
     CDS             5149..6147
                     /locus_tag="ACZPDN_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1298524.1"
                     /translation="MIKFNEQEKIDSVNGALALRPQINEFVDEICERGYSNICWLGVG
                     GTWASAMQATVHMKEMSAIETWAENAAEYLTTGNKRITKDTVVIISSVTGNTKEVVDA
                     IKKIKTEVGATVIAFVDAKEAILLDLADYKISYPINEQLKFFMVADRFMFNNGEFEDY
                     EDMYAEFDKYLAQDLVEVEKQAEPFAIEFAKKHWNDEMHYFVGAGNQWGATYSYAMCY
                     WEEQLWLKTKSITSNEFFHGMFEIVTKETPVTIYIGEDAQRPLSERVANFIPRICANY
                     TIIDSKDYELKGISEKYRKHLSHHVMHAVNNRIDVHMEIETRHPMEIRRYYRRLEY"
     gene            6252..6827
                     /locus_tag="ACZPDN_07600"
     CDS             6252..6827
                     /locus_tag="ACZPDN_07600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1298525.1"
                     /translation="MKKLLTILCSFVLCISLFGCQKETKNYIAKIEVKDYGTITLKLD
                     GKTAPITVQNFVDLSQSGFYDGLTFHRIMKGFMIQGGDPNANGTGGSDKTIKGEFKDN
                     GVKNNIKHKRGVISMARSSDYDSASSQFFIMQEDNDSLDGQYAAFGHVTKGMKIVDKI
                     CEDADPMDDNGTIAESKQPVIKTIKVSEIND"
     gene            complement(6988..7152)
                     /locus_tag="ACZPDN_07605"
     CDS             complement(6988..7152)
                     /locus_tag="ACZPDN_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298526.1"
                     /translation="MTEHTEKDVLMKCTKCGYEEEVPRWLIDELFPNEPEENYMMHCP
                     ECDHKMIVKK"
     gene            complement(7365..>7579)
                     /locus_tag="ACZPDN_07610"
     CDS             complement(7365..>7579)
                     /locus_tag="ACZPDN_07610"
                     /inference="COORDINATES: protein motif:HMM:NF016847.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1298527.1"
                     /translation="NYFIRYAGRPAMAENRITEYNKESKTVSWFYNDHKDEKRHDVTD
                     NVIDFINRLIIHIPDYHFLIIRCQCF"
BASE COUNT         2582 a         1033 c         1547 g         2417 t
ORIGIN      
        1 ccaatttcaa ggaaatataa gtgatcttca aaaatctatc aaggctattt gtgccaatgc
       61 gtttagtgaa accattcatg aagatgaaat ggaagtaaga atttatcact taccgacaca
      121 tcttcttgaa cagatgagtg tggataaata tgaaaaagaa gaagctacat ttgtgcgtat
      181 cgattcattt gaaaaacatg aattaggtga aaagatgatt caaatgtggg aacagctttt
      241 gaatgcatat gtggatttga aaaacaaagt gaattcccaa gctagatttt tagagattgg
      301 tcaagatgca gtccgtcatt attatgatgt tttagtgttt gaacagtcat atcacgatgc
      361 tcgtattgat gcgatgaata gtttagtgat ggatgtattg aatatagtga aaaatgcgaa
      421 aaacatcatt cttccagtaa attgtgcgta tgtattaacg cgtatgatta tttcaacagg
      481 tagaaatact tctttattga atcaatggga atcgaattat tcaaaagata ttcaagaatg
      541 tttacaaaca atgtcagaca tgatgatgaa tgagtcgatt attgcagatc agattattaa
      601 gcaaataaaa acagctacga atgttagttt gtcaaatatg aataagattt tcttgatgct
      661 gaatattcgt atgtacaaca aagaacttca aggtcaagat accatgggaa ttattttgag
      721 tcatggatat tcaacagctt cttccattgc ggatgcagca aactcattgt tgaatcatta
      781 tatttttgaa gcaatcgaca tgccactaga tacgccagtg gaagaaatta ctaaaaaatt
      841 aaatgaatat atcaacaata acatgcattt aaagaacatt atcttattag tggatatggg
      901 atctttagaa gagattggag atgtaattag tgacacgatg aatgtgggtg taatcaataa
      961 tatctctact tctttagcat taaatgttgg aatgaaaatg attcagcatc atgatttaga
     1021 aacaattcta agtgagtcgt gtgaagaaaa tcagtgtcat tataaaatat tatctgtcgc
     1081 aaaaaaagag aaggcgattg ccatcacaaa tgatgttagt gtttcagtaa gtgaaaaatt
     1141 atcgcatttg ttgcagaata gtcttcctaa aaaggtggat attaagttta tagaatatga
     1201 ctatgattgt ttacagaaaa ataaacatga tgatcctttg tttgaaaaat atgatgtggt
     1261 tttaatggta aaaccattga gtttgaaatt aaaaggggtt cgaagtgttt ctttagaaga
     1321 tattatgaat tttaaagata ttgatatatt aaattcaatc tttaaaccat atttagatga
     1381 ggatgaaatt gaagagttta atcaaaattt attaaagaat ttctcaattc aatctgtcat
     1441 ggaaaatcta acaattttaa atccacaaaa attattggat tttgttagtg aatccgtgca
     1501 aaacttacag ttggttatgc agaaaaaatt tcaaagtcgt acaatcattg gaatttatat
     1561 ccatatttgt ttccttgttg agcgtttggt aacaaagaca gcaattgaaa tatatgagaa
     1621 tttagatgat tttgcgaata accatagtga ttttattgag caggtgaatc aaagttttga
     1681 ttccatgttg aaaaattatc atgtaaagat gccaatttct gaaattgcat acttatatga
     1741 atatatagcg gatgatgaag atgaaagaaa ggtagcaagc gatgatgaat tttaaagata
     1801 aattatgtgg aatgaacagt cattatagat tttattcttt tgaacgtttt tttcaggaaa
     1861 tgaatcgttt aggtattaaa aatgtagaga tttggacggg tcctatgcat ttctatgtgg
     1921 actacaataa aaatcagagt gtagaacagc ttaaggaata cgcaaaaaaa tatgatgtaa
     1981 aaatcattgg tatttgtcca gaacaaacaa atccaaagcc aaacaatgtg gcaagtccaa
     2041 cacgtcaaga agatgtatat gcatattatt gtcgaatggt ggatatcgca aaagaagtca
     2101 atgcccaaca tatattaatc acaacaggct gggcttatta tgatgagtca aaagaagatg
     2161 catggaatcg ttcggttgaa atgatgcgta aggtttgtga ttatgcaaag gcaaatgata
     2221 ttttggtggc aatcgaagct ttacaaccag atgaatctgt tcttgtgaat agtgtagaac
     2281 aattaaaggc atatttggat gcggtaaatc atccacagtt aaaggtatgt attgattttg
     2341 gtgcaatggc acgagtcaat aatacaattc aagattattt tgatgcattt ggaaaagatg
     2401 tgatccatac acactttgtg gatggaaagc caacaggtca tttggcatgg tcggatggaa
     2461 caagagattt aaaacaagat cttcttgatt tagaggccaa tggataccat ggatatctgt
     2521 cactggaaac agcgacttct agatattatg aaaagccttg ggttgcagag gagacaactt
     2581 taaaagcttt cgaaaaatta ggaaaggagg attagtaatg agacacttta ttttggcaag
     2641 tcatggaacg ttctcacgtg gcatttacaa ctctgttcaa attattttgg gagaacagcc
     2701 aaatacgcat ataattacag cttatgttga agaaggacaa gatgtaaatg atttgattga
     2761 agaatcacta aaagatattc cagatacaga tcctgttatt gtatgtacag atgtatttgg
     2821 tggtagcgta aataatgaat ggatgaagcg tttaagtcgt ccaaatacac atttaattac
     2881 aggtatgaat cttggattat tgatgaatgt gtttatgcgt caagatgatg atattgaaaa
     2941 catgattcgt caaagtgtac aagaagccaa agatggcttg atttattgta acgatcttct
     3001 ttcaaacgac aatgaagaag aattttagga ggaagaaata tgattaagtt atgtcgtgta
     3061 gaccatcgac tactacatgg tcaggtagca ttctcttgga caaatgcagt acaagcagac
     3121 tgtattttgg ttgcaagtga tgaagtagtg aatgatgaaa tgtggaaaac aactttgaaa
     3181 ttaggtaaac cagcaggtgt taagttggtt atcaaagaca ttgaaaattc tgtaaaagca
     3241 ttaaacagcg gtgttacaga taagtacaag ttactaatcg tagttcaaac aattcatgat
     3301 gcatatcgat tagtacaaga agttccagcc attaagagta ttaacttagg tggagcaaaa
     3361 aaacaagacg gatatcggca aattagcaaa gctatatttg taggccctca agatgaagag
     3421 gatataaaag caatgttgga taagggtgtt gaagtagaaa tccgacaatt ggccagcgaa
     3481 agtagcgtgc cggtacaaag cgttctttaa caaacatagc aaggaggaga gcagaatatg
     3541 ttgttacaag caatcttgat tggtttggtt gcagcacttg gtgttttagg ggatcaaatg
     3601 ggttctcttt atatcaaccg tccaatcatc ttagctccat tagtagggct aatagttggt
     3661 aatttggagc aatccatcgt aattggtgca agcctagaat tattctttat gggggcagtt
     3721 agtgttggtg cctatatacc gcctgataca atcgttggtg gtgttttggc tacagcattt
     3781 gctttatcga ctggtagagg aattgaaaca gctgtaactt tggcaatgcc tatcgcatta
     3841 gtttctcaag cattaggtaa cttatgtaac gtatttaact caatcatctt acgttggaca
     3901 gatgattatg cagaaaaagg aaattataat ggtgtagtat ttacgcactg gttaattggt
     3961 atgatcacag tagtacgtcg tttcttgttg gtattatttg cttactattt aggaggcgat
     4021 gcagttggta atttgatcaa tgcaattcca gaattcgtta ctaatggtat ggcggcagct
     4081 gctggattat tacctgcact aggttttgcg atgttgatgc gtatgacaat taataaacaa
     4141 aatattcctt attacttctt aggatttatt ctagctgctt atatgggtgt tccagtatta
     4201 ggtgtagcaa tgattggtgt tatcttagta ttggttaaat ttaacttctt agataaacaa
     4261 gcagctacag aagcagaagc tgtacagggg gtgtctgacg atgatgactt ctaagattac
     4321 aaaaaaagat attacaaatt tagcatttaa ccaaggttct ttaggaatgg aattctcatg
     4381 gaactatgaa cgtcagatgc acattgcctt tgcgatgatg atgaatcaca acttgaaaaa
     4441 gatttatgaa ggtgatgaag aaggatatcg tgatgcatta gtaagacacg ttgaattctt
     4501 taatgtcact ccacaattgg ctccatttat tggtggtgta gttacttcaa tggaagaaat
     4561 gaaggctaga ggagaagttg atggagaagc gattgctagt atcaaaactg cattaatggg
     4621 tcctttatca ggtattggtg actcaatctt tgttggatgt ttacgtgtta tcgctgttgg
     4681 tatcggtatt tctttagcac aagctggaaa tatcatggga ccaatcttat atttcttact
     4741 ttataatatt ccatgcttca tcgtacgtta ctttggtgct cactttggat acaatttagg
     4801 tttcaattac ttacaaaaga tgcaagaaaa tggaatgatg gataaattgt tatctgctgc
     4861 aagtatcctt ggagttatgg tagttggttg tatgtctaaa gatatggttt ggacaacatt
     4921 aaatgttgaa ttatctagaa ttggtgacga agttacaaca ctacaatcaa ttttagacgg
     4981 aattatgcca ggattaattg gtttaggtgg tacttggttg tattacttct tgttgaaaaa
     5041 gaaagtcaat ccagtaattt taatttttgg aacaatgatc attggtgtaa tcggtgctta
     5101 ttttggcgta ttagcaggtt aattcattag atgaggagga acaataaaat gattaaattt
     5161 aatgaacaag aaaaaattga tagcgtgaat ggagcgttgg ctcttcgccc tcaaatcaac
     5221 gaattcgtgg atgaaatttg tgaacgtgga tattcaaata tctgctggtt aggtgttggt
     5281 ggtacatggg caagtgctat gcaggcaact gtacacatga aagaaatgtc agctattgaa
     5341 acttgggcag aaaatgctgc agaatactta actacaggaa acaaacgtat tactaaagat
     5401 acagttgtta ttatctcttc tgtaactgga aatacaaaag aagtagttga tgcaatcaaa
     5461 aaaatcaaaa cagaagttgg agctacagtt attgcattcg tagatgctaa agaagctatc
     5521 ttattagact tagcagacta caagatttct tatccaatta acgaacaatt aaaattcttc
     5581 atggttgctg accgtttcat gttcaacaat ggtgaattcg aagactacga agatatgtat
     5641 gcagaattcg acaaatactt agcacaagat ttagtagaag ttgaaaagca agctgaacca
     5701 ttcgcaattg aattcgctaa aaaacactgg aatgatgaaa tgcactactt cgtaggtgct
     5761 ggtaaccaat ggggagcaac ttactcatac gcaatgtgct actgggaaga acaattatgg
     5821 ttaaaaacta aatcaatcac atcaaatgaa ttcttccacg gtatgttcga aatcgtaact
     5881 aaagaaactc cagttacaat ttatattggt gaagatgctc aacgtccatt atctgaacgt
     5941 gttgctaact tcattccaag aatttgtgca aactacacaa ttatcgattc taaagattac
     6001 gaattaaagg gtattagcga aaaatatcgt aaacacttgt ctcaccatgt aatgcatgct
     6061 gtaaacaaca gaattgacgt tcacatggaa atcgaaactc gtcacccaat ggaaattcgt
     6121 cgttactaca gaagattaga atactaagag ctaattatag ttgaatgcaa ggatgtttac
     6181 ctgtcttttg atgggaaaca tccttttttt ctttggcttg tcctgttaaa ataaggtaga
     6241 ggtggaaaat aatgaaaaaa ctattaacga ttctatgttc gtttgtttta tgcatatctt
     6301 tatttgggtg tcaaaaagag acgaagaatt atattgctaa aattgaagtg aaggactatg
     6361 gaacaattac attaaagttg gatgggaaaa cagctccaat tactgtacaa aactttgtag
     6421 acctttccca atccgggttc tatgatggat tgacattcca tcgtatcatg aaaggtttta
     6481 tgattcaggg aggagatccg aatgcgaatg gtacaggtgg atcggataag acaatcaagg
     6541 gtgaatttaa ggataatggt gtaaaaaata atatcaagca taagcgtggt gtgatttcga
     6601 tggcaagaag ttcagattat gattcagcca gttcacaatt cttcattatg caagaagaca
     6661 atgattcatt agacggtcaa tatgcagctt ttggacatgt tactaaagga atgaagattg
     6721 tagacaaaat ttgcgaagat gcagatccaa tggatgataa tggtacgatt gctgaaagta
     6781 aacagcctgt tattaaaaca atcaaagtat ctgaaataaa cgactagaga aatctagttg
     6841 ttttttatat attaagattt tggttttgga atagagaaat gcggaatgtg aaatagtgtt
     6901 aaaatatcac aagatatgaa ttgtcaggga cgacaaaaag gactattgaa aatagccaac
     6961 attttgtatt catctttagt tgttgaatta tttttttaca atcatcttat gatcgcattc
     7021 aggacagtgc atcatatagt tttcttcagg ttcatttgga aataattcgt cgatcaacca
     7081 tctaggtact tcttcttcgt aaccgcattt tgtacatttc atcaatacat ctttttctgt
     7141 atgttctgtc atttctgttg tccttgaatg gattatatgt ataagtatac tgcgtaatgg
     7201 ctccacattt acattgaatt ggatctctat taaaagaatc aatcaaatga gtgcggtatc
     7261 tgaatttact gagtttgttt ttaagggctt tagttcttgt tttacgagaa taatccttat
     7321 tctttttgat tccaagaagt gcatgtactt tatctatggt tttcttagaa gcattggcat
     7381 cgaataatta agaaatgata gtccggtata tgtataataa ggcggtttat aaaatcaatc
     7441 acattatctg tgacatcatg acgtttttca tccttatgat cattgtagaa ccagctgaca
     7501 gtctttgatt ctttattgta ttctgtgatt ctattttcag ccatggcagg cctacctgca
     7561 tagcgaataa aatagttta
//
LOCUS       DBMDWL010000101         7501 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3660_length_7501_cov_2.144189, whole genome shotgun sequence.
ACCESSION   DBMDWL010000101 DBMDWL010000000
VERSION     DBMDWL010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7501)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_7501_cov_2.144189"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(139..2769)
                     /gene="alaS"
                     /locus_tag="ACZPDN_07615"
     CDS             complement(139..2769)
                     /gene="alaS"
                     /locus_tag="ACZPDN_07615"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR1298528.1"
                     /translation="MKQLTGNQIRQMFLDYFKSKGHMIEPGASLIPHNDPTLLWINAG
                     VAALKKYFDGSEKPASNRIANAQKSIRTNDIENVGRTARHHTFFEMLGNFSIGDYFKD
                     EAIQFAWEFLTSEEWMGIDKDKLYVSVYTDDARAYEVWTTICGVDPSHILKTDDNFWE
                     IGKGPGGPDSEIFFDRGEKYDPEGLGEKLFFDEMENDRYVEVWNVVFSQYDCDPSIDR
                     KDYKELPQKNIDTGMGLERLVALVQDGETNFDTDLFLPIIRATEAMAKHPYEGEYKMA
                     YRVIADHVRTVTFALSDGANFSNSGRGYVLRRVLRRAVRYGLKLGLDEPFLYKLVPVV
                     ADLMKDFYPYLQEHVEFNQKLIKVEEETFKKTLKVGQALLDDEISKAKDGKLSGEVVF
                     KLYDTYGFPFELTQEIAEESGISVSHDEFDAEMNKQKERARNARNVKDSFASQNEELM
                     NFNEPSEFIGYDHLTCDGKIIALFNAEGKMVDSLEDEGMIILDETCFYAESGGQVADK
                     GTFSADGVEVEVLDVQKTRNKQHIHTVKINSGVLEKGMALHGEVNVKDRLATTANHSC
                     THLLQSALVKVLGDHIHQAGSYNCPEYLRFDFNHYEKVTAEQLAEVERIVNEYISAAY
                     PVTKEVMPIEEAKKSGATALFDEKYGDTVRVVTMGDVSKEFCAGCHVENTAQIGLCKI
                     ISEESIGSDSRRITAKTKFAAYEDFASEHAMLENIADSAKQKGIKNIDTKVEAAYKTM
                     HDMQKEIDNLKNQIFTLKSKEWATEAKDFGKVNVLIKSVSGMDAGALKDIVSNLKAND
                     DKMVVFFVNTNGEKVVFVSGAGKEAVKAGVHAGQLVKKAAQICSGNGGGKPDMAQAGG
                     KDASKVDEAINAITEELKSL"
     misc_binding    complement(2803..2998)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3008..4288)
                     /locus_tag="ACZPDN_07620"
     CDS             complement(3008..4288)
                     /locus_tag="ACZPDN_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR1298529.1"
                     /translation="MKKYEVIYNTYRESILSGILKYGDRVDSIRECVKKMNVSKTTVE
                     LAYNKLVLDGFISSKEKVGYVVSMNPERVLLHHEILDYSSSHKEKEYDYDFRIQSVSI
                     DSFETSIWKRYIKGVLNDKRLMSSYGLAQGEYGLRQALCKYVYQNRNILCFPEQMVIG
                     SNYQSLLYIFCGMLDKQKVIGLSTLVDEQAKQVFLSYGFSVKYLDPDHFIEDIQTKCV
                     DVVYVNTTCFSKNRQSMSEKLRKKLVGLSHILILEDDYNGELVYASKIKTSLCSMSNH
                     VIYFSSFSRLLLPSLRIGYMILNEEYTKKYCASFFGPTASKLEQVAFSQYIVDGYLQK
                     HLRKLVREYREKHLYLKKLLDTYLGCSYILDETYMAYWIKLDVDMEKFKSLCESNLMG
                     VSYSSSGIGLSFASMDKELMKDGVIRLAELVKEC"
     gene            4384..4881
                     /locus_tag="ACZPDN_07625"
     CDS             4384..4881
                     /locus_tag="ACZPDN_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1298530.1"
                     /translation="MKNKQTYEIVLTGLGMALVFVATMFIKVPNALDGYFNLGDGFIL
                     LFASFLNPFESFLIGGLGSALADVAGGYGHYFFFTLVIKGLEGIVVSLLMKNYSKPKQ
                     ISSYVLGSIIMVFGYFIAKYMLKGSWIVAASEIPANIVQGVAGIVIALVAYPMVKKIV
                     QDKLN"
     gene            complement(4950..5249)
                     /locus_tag="ACZPDN_07630"
     CDS             complement(4950..5249)
                     /locus_tag="ACZPDN_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298531.1"
                     /translation="MGFFDYMDTIEKPTIDCIRLIYKAINVKYDELIDMAVEPNSKNY
                     NKWMQTLDCLKASEDLIIECIGKNAITDMEWLQLKCNIYKFQVKYGGLKYLNVEA"
     gene            5774..6886
                     /locus_tag="ACZPDN_07635"
     CDS             5774..6886
                     /locus_tag="ACZPDN_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIR1298532.1"
                     /translation="MDEFNKEVCKLYKNIDEQIEYLKMFKKIIINENERSILEDRNYI
                     SVINPYKEFFATNQIVKNIEGYTKKIHIYESETPIQNILSVVKYDDKISDYFFRTIGQ
                     FERKFKNVLINAICELYVHNSQMPNESLKCLEYITEIEKFINQYTIELTLNNGSTYTC
                     LNKPYLIDAIQRNVVVFPKFATNFPNSLSKKGYVYNEFVLENRFMILKKLYDIGTGDK
                     SSSKNILLQHYYNSQKMLPLWVIPNALTLGELNVLFSMLNMSTQKQICAKLMNVDITK
                     IKEKNVSTFMGYVENIRRIRNVINHYEPLIPFLLNNIKEKHLKDSQIIKTIEFLATYS
                     EPIIITMPYIPVTDYNKKKVAVLKKVQQVMQKSNKL"
     gene            7093..7452
                     /locus_tag="ACZPDN_07640"
     CDS             7093..7452
                     /locus_tag="ACZPDN_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298533.1"
                     /translation="MLFVYKDRKAIFIADRGYESINSFEKIKLSGNKYLVRIKNIYST
                     GILRSFGPFSYDEFDVQVKRILTRKQTNEIKAHPEIYKFVPQNQRFDYFGDTPFYDFE
                     SRLVRFKITEDTYENAS"
BASE COUNT         2613 a         1520 c          972 g         2396 t
ORIGIN      
        1 tttaatattg ccatctgaac actagaaaaa atcggaaatt ctttaataga gtttccgatt
       61 tcgtttattt aagccatttt atttttcatg cgtaactact aacaaaaagg atatgcacat
      121 ggcataccct aatatttttt ataagctttt taattcttct gtgattgcat tgattgcttc
      181 atcaacctta gaagcatcct taccaccggc ttgagccata tctggctttc caccaccatt
      241 accagaacag atttgagccg ctttcttaac caattgaccc gcatgtacac cagccttaac
      301 agcctcttta cctgcaccag acacaaagac aaccttttca ccatttgtat tcacaaagaa
      361 tacaaccatc ttgtcatcat tcgcttttaa gttagaaaca atatccttta atgcacctgc
      421 atccatgcca gaaacagatt tgatcaatac attgacttta ccaaaatcct tagcctctgt
      481 agcccactct ttagacttca aagtaaagat ttgatttttc aagttatcga tttccttttg
      541 catatcatgc atcgtcttat aagcagcctc aacctttgta tcaatattct taataccctt
      601 ttgttttgca ctatccgcaa tattttctaa cattgcatgt tcagatgcaa agtcttcata
      661 agccgcaaac ttagttttcg ctgtaatacg acgagaatcc gaaccaatac tttcctcact
      721 aatgatctta cataaaccaa tctgagctgt attttctaca tgacatcctg cacagaattc
      781 cttcgataca tctcccattg taacaacacg aactgtatct ccatactttt catcaaataa
      841 tgcagttgca ccagactttt tagcctcttc aatcggcata acctctttag taactggata
      901 agccgcacta atatattcat tcacaatacg ttccacttca gccaattgtt cagctgtaac
      961 cttttcataa tgattaaagt caaaacgcaa atattcagga caattgtaag aaccagcttg
     1021 gtgaatatga tcccccaaca ctttaactaa agccgactgc aacaagtgtg tacaagagtg
     1081 gttcgctgta gttgccaaac gatctttgac atttacttca ccatgtaaag ccataccctt
     1141 ctctaataca ccactattga ttttaactgt gtggatatgt tgtttattac gcgttttttg
     1201 aacatctaat acttctactt ctacaccatc tgcactaaat gttcctttat cggcaacctg
     1261 tccaccactt tctgcataga aacaagtttc atctaaaatg atcattcctt catcttctaa
     1321 agaatcaacc atcttaccct cagcattaaa taacgcaatg attttaccat cacaagttaa
     1381 gtgatcataa ccaataaact cactaggctc attgaagttc atcaactctt cattttgaga
     1441 cgcaaaagaa tcctttacat tacgcgcatt acgagcacgc tctttttgtt tattcatctc
     1501 tgcatcaaat tcatcatgac ttacactgat tccactttct tccgcaattt cttgtgttaa
     1561 ttcaaatggg aatccatatg tatcatacaa tttgaagaca acttcaccac tcaacttacc
     1621 atctttagcc ttgctgattt catcatccaa taaagcttga ccaaccttta aagtcttctt
     1681 gaaagtttct tcttccacct taatcaattt ttgattgaac tctacatgct cttgtaagta
     1741 aggatagaaa tccttcatta aatctgcaac aacaggaact aatttatata agaatggttc
     1801 atccaaacct aattttaaac cataacgaac cgcacgacga agtacacgac gaagcacata
     1861 tccacgacca ctatttgaga agttagctcc atcactcaat gcgaaagtca ctgtacgaac
     1921 gtggtccgca ataacacgat atgccatctt atattcccct tcataaggat gtttagccat
     1981 agcttcagtg gcacgaataa taggcaagaa taaatcagta tcgaagtttg tttcaccatc
     2041 ctgaactaag gcaaccaaac gctcaagacc catacctgta tcaatattct tctgaggaag
     2101 ttccttataa tctttacgat caatacttgg atcacaatca tattgagaga atacaacatt
     2161 ccatacttct acataacgat cattttccat ttcatcaaag aataattttt ctcccaaacc
     2221 ttcaggatca tatttttctc cacgatcaaa gaaaatttca ctgtcaggtc caccaggtcc
     2281 cttaccaatt tcccagaagt tatcatctgt ctttaaaata tgacttggat ctacaccaca
     2341 aattgtagtc caaacttcat aagcacgcgc atcatctgta tatacagaca catacaattt
     2401 gtccttatca atacccatcc attcttcgct tgttaaaaat tcccatgcga actggatagc
     2461 ttcatctttg aaataatctc caattgagaa gtttcctaac atttcaaaga atgtgtggtg
     2521 acgagctgta cgcccaacat tttcaatatc atttgtacga attgacttct gcgcattcgc
     2581 aatacgattt gaagctggtt tttcactacc atcaaaatat tttttcaaag ccgcaacacc
     2641 cgcattgatc cataataaag ttgggtcatt gtgcggaatc aaacttgcgc ccggctcaat
     2701 catatgtccc tttgatttaa aataatccaa gaacatctga cgaatctgat taccagttaa
     2761 ttgtttcatc tttctttcct ccaataaaaa aacgctccta tttctaggaa cgtataatta
     2821 cgcggtacca tcctagttca ttttcccaaa aaagaaaatg ccctcaattc actcgttaac
     2881 gccgactacg tttcaactcc aaagtagctt caaaaataaa ttcctgaaag gcttgcacca
     2941 aacgccctct ctctagacaa gaaaattatt ttctacttat cttcttcact gtcgaatcgt
     3001 taaaattcta acactctttc accaattctg caagacgaat cacaccatct ttcatcaatt
     3061 ctttatccat actcgcaaaa gataagccta tcccactact tgaataacta acacccatta
     3121 aatttgattc acacaaagac ttgaactttt ccatatccac atccaacttg atccaatacg
     3181 ccatatacgt ttcatccaaa atataagaac aaccaagata tgtatccaat aacttcttta
     3241 aatacaaatg cttctctcta tattcacgca ccaatttcct taaatgcttc tgtaaatacc
     3301 catccacaat atactgacta aaagccacct gctctagttt actagccgta ggaccaaaga
     3361 aagaagcaca atacttcttt gtatattcct catttaaaat catatatcca atacgaagac
     3421 taggaagtaa aagtctagaa aaagaagaaa aataaatcac atgattcgac atagaacata
     3481 aactcgtctt aattttagaa gcatacacaa gctccccatt ataatcatct tctaaaatca
     3541 aaatatgact tagtccaacc aacttttttc tcaacttttc agacatagac tgacgattct
     3601 tactaaaaca agtcgtattc acatacacaa catccacaca ctttgtttga atatcttcaa
     3661 taaaatgatc cgggtctaaa tatttcacag aaaagccata acttaaaaac acttgtttag
     3721 cttgttcatc caccaaagtt gacaagccaa tcaccttttg tttatccaac attccacaaa
     3781 atatatacaa caaagactga tagttcgaac caatcaccat ctgctcagga aaacacaata
     3841 tatttcgatt ttgatataca tacttacaca aagcctgtct taaaccatac tccccttgag
     3901 ctaaaccata acttgacatt aatctcttat cattcaatac accctttata tatcgtttcc
     3961 aaatcgatgt ctcaaatgaa tcaatcgaaa ccgactggat tcgaaaatca taatcatatt
     4021 ccttttcttt gtgacttgaa gaataatcca agatttcatg atgcaataaa accctttccg
     4081 gattcatact cacaacatat ccaacctttt ccttacttga aataaaccca tctaacacaa
     4141 gcttattata agctagctcc accgttgtct tagacacatt catcttcttg acacattcac
     4201 gaatcgaatc gacacgatcc ccatatttta agattccaga aagaatcgac tccctataag
     4261 tattataaat cacttcatat ttcttcataa actgtcccct atttattttt tataattgta
     4321 tattttttta aatacagaaa caagtataat agtcattaag ttgtaagaaa gaaggaaatg
     4381 atcatgaaaa ataaacaaac ctatgaaatc gtattaacag gacttggcat ggcactagtc
     4441 tttgtggcaa ccatgtttat caaagtacca aacgcactcg atggatattt caatttaggc
     4501 gatggattca tcctattatt cgcaagcttt ttaaatccat ttgaaagctt cttaattgga
     4561 ggactaggaa gtgcattagc cgatgtagcc ggaggatatg gacactactt cttctttacc
     4621 ctagtcatta aaggacttga aggaattgtc gtatctctac tgatgaaaaa ttactctaaa
     4681 cccaaacaaa tctcaagcta cgtattagga tctatcatta tggtctttgg atacttcatt
     4741 gccaaatata tgttaaaagg aagctggatc gttgccgcta gtgaaattcc agctaacatt
     4801 gtacaaggtg tagctggcat tgtgattgca ctagtcgcct atccaatggt caaaaaaatc
     4861 gtacaagata agttaaacta aagcctggaa attccaggtt tttttatttc caaataaaaa
     4921 aaggatatgc tcagcacata ccctaatatc tatgcttcta cattcaaata ttttaagcca
     4981 ccatatttta cttgaaactt ataaatatta cacttcaatt gaagccattc catatccgta
     5041 atcgcattct ttccaataca ttctataatc aaatcttcag atgcctttaa acaatccaat
     5101 gtctgcatcc atttattata atttttacta tttggttcaa ccgccatgtc tatgagctca
     5161 tcatacttca cattaatagc cttatatatc aatcgaatac aatcaatcgt aggcttttca
     5221 attgtgtcca tataatcaaa aaatcccata ataaatcatc ctttcgtttt ttatggttta
     5281 ataatacacc tcgaaaaaac gtttgtatag aaaaacaccc aaataaatgc aatattatat
     5341 gtctaaaaaa agcggtttct ttaagaaaat aattaaaatt ttacgatatt aaaacaattg
     5401 ctttttgcgg tattaaccat ataatgcgtg tgcaaaaaga agcttttaaa agcttgggct
     5461 ggccggatcc atatagtggc gggtaagaca cccgccattt ttttagaaag ttgaggaagc
     5521 atgaaagagt taagcgtttt tgttgacgaa tctaaagatt ttggtgaata tgattatcat
     5581 gctccttatt atattatttc attagtcttt cataatcaaa atatgaatat atagatgcac
     5641 aagatataat tgaattgatc aaatacttca agacgatgga atataaaatc aacatttttt
     5701 ataatttttg ctctatatcg aagtctagac agaatcatga taaaatatgt aatatagaaa
     5761 tggaagtatt caaatggacg aatttaataa agaagtatgt aaactatata aaaacattga
     5821 tgaacaaatt gagtatttga aaatgtttaa aaaaatcata ataaacgaaa atgagcgatc
     5881 tatattagag gatagaaatt atatatctgt aatcaatcca tataaagaat tttttgcaac
     5941 taatcagatt gttaaaaata ttgaagggta tacgaagaaa attcatattt atgaatcgga
     6001 aacacctata caaaatatac ttagtgttgt taaatatgat gataaaattt ctgactattt
     6061 ttttagaacc attgggcaat ttgaaaggaa atttaaaaat gtattaatta atgcaatttg
     6121 tgaattgtat gttcataatt ctcaaatgcc aaatgaatct ttaaaatgct tagagtatat
     6181 cactgaaatc gaaaaattta taaaccaata cacaattgaa ctaacactta ataacggaag
     6241 tacatatact tgtttaaaca aaccatattt aatagatgca atacagagaa atgtagttgt
     6301 ttttcctaaa tttgcaacaa attttccaaa ttcattgtct aaaaaaggtt atgtgtataa
     6361 tgaatttgtt ttggaaaatc gatttatgat attaaaaaaa ttgtatgata taggtactgg
     6421 agataaaagt tcatcaaaaa atatactttt acagcattat tacaattctc aaaagatgct
     6481 acctttatgg gttattccta acgctttaac cctcggggaa ctaaacgttt tgttttcgat
     6541 gttaaatatg tcaacacaaa aacaaatctg tgcaaaacta atgaatgttg atattacaaa
     6601 aattaaagaa aagaatgtat ctacctttat gggctatgta gaaaatattc gaagaataag
     6661 gaatgtgatt aatcattacg aaccacttat cccgttttta ttaaataaca taaaagaaaa
     6721 gcatcttaaa gattcacaaa ttataaaaac aatagaattc cttgctactt actctgagcc
     6781 aataattatt actatgccat atattccagt tacagattac aacaagaaaa aggtagctgt
     6841 tttgaaaaaa gttcaacaag ttatgcaaaa gagcaacaag ttgtaaagtt gctcttttat
     6901 tctaaacaaa aaaagagaat ggacgtatcc actctccctg tcagccaatg ggccaacctt
     6961 cgtcatgagt aatataccac actttttgaa aaatgcaaat aaattttacg caattccccc
     7021 ttcccccaca ataagccata agtgtttttt taatattcac tattttccgc gatagaataa
     7081 tcacatagaa atatgttatt tgtttataaa gacagaaaag ctatattcat tgcagacaga
     7141 ggatatgaat ccatcaattc ctttgagaag attaaattat ctggaaataa gtatctggtc
     7201 cgaatcaaga atatttattc cactggtata ttacgtagtt ttggaccatt ttcatatgac
     7261 gaatttgatg tgcaagtcaa acgcatattg actagaaaac agacgaatga aataaaagct
     7321 catcctgaaa tctataagtt tgtgccacaa aatcagcgat ttgattattt tggtgatact
     7381 ccattctatg attttgaatc tcgattggtt cgttttaaaa ttacagagga tacatatgag
     7441 aatgcatctt aacaaattta gatgaaaatg aattcagtat gcaagatatc aaaaagtcgt
     7501 a
//
LOCUS       DBMDWL010000102         7491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3668_length_7491_cov_3.115156, whole genome shotgun sequence.
ACCESSION   DBMDWL010000102 DBMDWL010000000
VERSION     DBMDWL010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_7491_cov_3.115156"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="araD"
                     /locus_tag="ACZPDN_07645"
     CDS             <1..562
                     /gene="araD"
                     /locus_tag="ACZPDN_07645"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HIR1298534.1"
                     /translation="YDVMTRDDIVLVDLDGNIVEGNLRPSSDLDTHLEFYRNFPNIGG
                     VVHTHSTWATSFAQAGKDIIPLGTTQADYFHGAVPCTRLMTEEEIHGDYELETGKVIV
                     EEFKTRNIDPDRVPGVLVHSHGPFTWGTDGFNAVHNAVVLEECAKMNAIASLLSNNEI
                     DSMQQVLLDKHFNRKHGPGAYYGQAK"
     gene            546..1394
                     /locus_tag="ACZPDN_07650"
     CDS             546..1394
                     /locus_tag="ACZPDN_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HIR1298535.1"
                     /translation="MDKLNREYRLGLYEKSMPSTLTWVEKLTIAKKSGFDYLEISIDE
                     SDAKLARLDQPTDEIKEAIQKTGVPISSMCLSGHRKYPLGSHDKEIQKKSLEIMKKAI
                     DFAADLGIRIIQLAGYDVYYEDGDLYTNHDFETNLKKCVEMAAKKGIVLGFETMETPF
                     MDTVEKAMRYVNLVSSPYLGVYPDIGNLKNASLLYGVDVDEDLMCGKGHIFASHLKET
                     VPGKYREIPFGTGHTEFVKNIKTLKRLGVRMFVGEFWYVNNEDWQDVVKNAGVFLRDK
                     LDKGFE"
     gene            1395..2213
                     /locus_tag="ACZPDN_07655"
     CDS             1395..2213
                     /locus_tag="ACZPDN_07655"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1298536.1"
                     /translation="MNIKELELHANRIRKNILTEVHSANSGHPGGSLSGADILTEIYF
                     EQMDINKENIDSIDRDRFVLSKGHASPLLYGTLKEKGLLEDDLTTFRKINSNLQGHPN
                     MNEVAGVDMSTGSLGQGISCAVGMAIVNKLVDKNNHRIYCLLGDGECEEGQVWEACMA
                     AAHYKLDNLCAVLDYNHLQIDGNIDDVISPEPFASKFESFGWNVLDVNGHDFNELRNA
                     FEQAKQVEDKPTMIIAHTIKGKGVSYMENNYAWHGAAPNKEQYEQAMKELGGLE"
     gene            2213..3139
                     /locus_tag="ACZPDN_07660"
     CDS             2213..3139
                     /locus_tag="ACZPDN_07660"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1298537.1"
                     /translation="MAKEATRNAYGKALAELVVERDDVLVLDADLTKSTKTIEAKKAR
                     PEHHFNMGIAEGNMMAVAAGMAASNKVVYASSFAMFAAGRAFEQIRNSICYPNLNVKV
                     CATHAGITVGEDGASHQCIEDIALMRSIPNMKVFVPCDQYQAKAIVKAVADIDGPCYV
                     RLGRGAVEEVYDENYKFELGKGKVLREGQKVALVATGMMVQEALKAADILSKEGILVT
                     VVDMPCIKPIDEELIEEIAKSHEIIITCEEHNVYGGLGSAISEVTSKKSPVRMEMIGM
                     QDTFGESGTPNELLAKYGLNADHIVEKVKECL"
     gene            3130..3909
                     /locus_tag="ACZPDN_07665"
     CDS             3130..3909
                     /locus_tag="ACZPDN_07665"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1298538.1"
                     /translation="MSLKKIKLIVADLDGTLLHDDKSLDRNIKSVLIEKNVPLTFVSG
                     RNVHIIQDYIKELNITLPYITNNGANMFLGDTCIYECPIDSSELKTCLNILVANKVAF
                     LAYTNQTIYSVGYQEGLDAFKNRLIGKCEIVENANIEDIVSESIFKVVMIHPNMEKIK
                     NQLNSVCDKTLCMQSEGIIYTLTNKDSTKGKTLLKLLKYLDMEPSSVLAFGDNYNDIS
                     MFEVADGVAVENAQESLKEVAKYLTKSNEDDGVSYFIQNKL"
     gene            4068..4997
                     /gene="cysK"
                     /locus_tag="ACZPDN_07670"
     CDS             4068..4997
                     /gene="cysK"
                     /locus_tag="ACZPDN_07670"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018578801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1298539.1"
                     /translation="MTVYKAISDLIGNTPLVELTHIEEKEGLDASVVAKVEFFNPAGS
                     VKDRIAKKMIEDAEKKGLIKPGATLIEPTSGNTGIGIASVAAAKGYKAIMTMPETMSV
                     ERRNLLKAYGAKVVLTDGKAGMKGAIAKAQELAAATPNSFIPSQFENPSNPQAHYEST
                     GPEIWKDTEGKVDIFVAGVGTGGTVSGTGKYLKDQNPNVKVVAVEPATSPVLSQGHAG
                     PHGIQGIGAGFVPNTLDTSVYDEVFTVTNEQAYETGRLIAHNEGMLVGISSGAATYAA
                     IQIAKRPENKGKTIVVLLPDTGERYLSTPMFAE"
     gene            complement(5020..6000)
                     /locus_tag="ACZPDN_07675"
     CDS             complement(5020..6000)
                     /locus_tag="ACZPDN_07675"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HIR1298540.1"
                     /translation="MPINIPDALPAKDILESEKIFAIEDKSAHRQRIRPLKIVILNLM
                     PKKIETETQILRLISKSALQVDIDFMMVKNHVSKNTSQDHLVKFYTYFEKLKNNYYDG
                     MIITGAPVEHLEYEDVDYWNELEEIMEWSKTHVYSTLHICWGAQAGLYYHYGVQKHIL
                     PEKVFGIFPEQLVDEYDFLTNGFDEIHYTPHSRHTAIDEDAVKKVKCLSILSKNDEIG
                     SNIIVTKDYRQIFILGHLEYGKETLKDEYFRDLKANKPIHIPFNYFPDDDPEKEPILK
                     WRSHANLLYRNWLNYVYQLTPFEIEEIGKIKEPIGTGSREDHGYAKTFTK"
     gene            complement(6080..7357)
                     /locus_tag="ACZPDN_07680"
     CDS             complement(6080..7357)
                     /locus_tag="ACZPDN_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1298541.1"
                     /translation="MKTETKCLHAGYEPKNGEPREVPIYQSTTFKYDSSLQMGRLFDL
                     EDSGYFYSRLQNPTNDIVASKIAALEGGIAAMLTSSGQAANFFAVFNICEAGDHLIAS
                     SAIYGGTYNLFGVTMKKMGIDCTFIDPEISEEDLQKEFKPNTKCVFGETITNPTVRIF
                     DIEKFAKVAHANGVPLIIDNTFATPIMCQPIQWGADIVTHSTTKYMDGHASCVGGAIV
                     DSGNFNWLEHADKYPGLTTPDESYHGIVYAEKFGKLAYITKATSQLMRDLGSIQAPQN
                     AYYLNLGLESLAVRMKAHCANALAVAKYLEANEDVAWVKYADLESDPHHELAKKYCPN
                     GTCGVLSFGLKADRDQTEKFMDSLKLASIVTHVADAKTCLLHPASHTHRQMSEEQLKE
                     AGVAPDLIRFSVGLEDAQDIINDLQQALDAMKA"
BASE COUNT         2483 a         1072 c         1563 g         2373 t
ORIGIN      
        1 atatgatgtc atgacgcgtg atgatattgt gcttgtggat ttagatggaa atattgtaga
       61 aggaaatcta cgtccaagct ctgatttaga tacacatttg gaattctatc gtaactttcc
      121 aaatattgga ggtgttgttc atacgcattc cacttgggca acgagctttg cacaggcagg
      181 aaaagatatc attccattag gaactacaca ggcagattac tttcatggag ctgttccttg
      241 tactcgtttg atgacggaag aagaaattca tggagattat gaattggaaa caggaaaagt
      301 gattgtagag gaatttaaaa cacgcaacat tgatccagac agagttcctg gtgtattggt
      361 acattcacat ggacctttca cttggggaac ggatggattc aatgccgtac ataatgcggt
      421 tgttttagaa gaatgtgcaa agatgaatgc gatcgcatct ttattaagca acaatgaaat
      481 tgattccatg cagcaggttc tattagataa acactttaat cgtaaacatg gaccgggagc
      541 ttattatgga caagctaaat agagaatata gactaggtct atatgaaaag agtatgccaa
      601 gtacacttac ttgggtagaa aaattaacaa ttgccaagaa gagtggattt gattatttgg
      661 aaatcagtat tgatgaaagt gatgcaaaac ttgcaagatt ggatcagcca acggatgaaa
      721 tcaaggaagc cattcaaaaa acgggggttc ctatttcttc aatgtgttta agtggacatc
      781 gtaaatatcc acttggatca catgataagg agattcaaaa gaagtcattg gaaatcatga
      841 agaaagcgat tgattttgcg gcagatctag gaattcgcat tattcagtta gctggatatg
      901 acgtctatta tgaagatggg gatctataca caaatcatga ttttgaaaca aatctaaaga
      961 aatgtgtgga aatggcagct aaaaaaggaa tcgtattagg gtttgaaaca atggaaacac
     1021 catttatgga tacagtagaa aaggcaatgc gctatgtaaa tctagtaagc agcccatacc
     1081 taggtgtata tccagatatt ggaaatttaa agaatgcatc cttattgtat ggtgtagatg
     1141 tagacgaaga tttgatgtgt ggaaaaggac atatctttgc gagtcattta aaagaaactg
     1201 ttccaggtaa atatcgtgaa attccatttg ggacaggtca tacagagttt gtgaaaaata
     1261 ttaaaacatt gaaacgacta ggcgtgcgta tgtttgtcgg agaattttgg tatgtgaaca
     1321 atgaagactg gcaagatgtt gtaaagaatg caggtgtgtt cttaagagat aagttagata
     1381 agggatttga gtaaatgaat ataaaagaat tagaacttca tgcgaatcgt attcgtaaaa
     1441 atatattaac cgaagtacat agtgctaact caggacatcc aggaggatcc ttgagtggtg
     1501 cagatatttt aacagaaatc tattttgaac agatggatat taataaagaa aatatagatt
     1561 caatcgatcg tgatcgtttt gtattaagta aaggacatgc gagcccatta ttgtatggaa
     1621 cgttaaaaga aaagggattg ttagaggatg atttaacgac atttagaaag atcaattcaa
     1681 atttacaagg acatccaaat atgaatgaag ttgcaggtgt agatatgtct acaggatctt
     1741 taggacaagg tatttcttgt gctgttggta tggctattgt gaataaatta gtagataaga
     1801 ataatcatcg aatctattgc ttattaggtg atggtgaatg tgaagaaggt caagtatggg
     1861 aagcttgtat ggcagccgct cactataaat tggataattt atgtgccgtt ttagactaca
     1921 atcatttaca gattgatgga aatattgatg atgtaatttc gccagaacct ttcgcaagta
     1981 aatttgaatc ttttggatgg aacgtattgg atgtaaatgg acatgatttt aatgaattaa
     2041 gaaatgcgtt tgaacaagct aaacaagttg aggataaacc aactatgatt attgcccata
     2101 caatcaaggg taaaggtgtt tcttatatgg aaaataacta tgcatggcat ggagctgcac
     2161 caaacaaaga acagtatgaa caggcaatga aggaattagg gggtcttgaa taatggcaaa
     2221 agaagcaaca cgtaatgcct atggaaaggc tttggcagaa ttagttgtag aaagagatga
     2281 tgttttagta ttagatgcgg atcttacaaa atctacaaag acaatcgaag ccaaaaaggc
     2341 aagaccggaa catcatttta atatgggaat tgccgaagga aatatgatgg cagtcgctgc
     2401 aggtatggct gcaagtaata aggttgttta tgcatcaagt tttgcgatgt ttgcggcagg
     2461 acgagctttt gaacaaattc gtaatagtat ttgttatcca aacttgaatg taaaagtatg
     2521 tgcaacgcat gctggaatta cagttggaga agatggtgca agccatcagt gtattgaaga
     2581 tattgcactt atgagaagta ttccaaatat gaaggtgttt gtaccatgtg atcaatatca
     2641 agcaaaagca attgtaaagg cagtggccga tatagatggg ccatgttatg taagacttgg
     2701 gcgtggtgca gtggaagaag tatatgatga aaattataag tttgaattgg gtaaaggaaa
     2761 agttttaaga gaaggtcaga aagtggcatt ggttgcgaca ggcatgatgg tacaagaggc
     2821 attaaaagca gcagatattt tatcgaaaga aggaattttg gttacggttg tagatatgcc
     2881 ttgtatcaaa ccaatcgatg aagagttgat tgaagaaatc gcaaaatccc atgaaatcat
     2941 catcacttgt gaagaacaca atgtgtatgg aggtttagga agtgccatta gtgaagtaac
     3001 ttcaaaaaag agtccggtgc gcatggaaat gattggtatg caggatactt ttggagagag
     3061 tggaacaccg aatgaactac tagctaaata tggtttgaat gcggatcata ttgttgaaaa
     3121 ggtaaaggaa tgtctttaaa aaaaattaaa ttgattgttg ctgatttaga tggcacgctt
     3181 ttgcatgatg ataaaagttt agatcgaaat attaaaagtg ttctgattga aaagaatgtt
     3241 ccacttactt ttgtatcggg tcgaaatgtg catattattc aggattatat aaaagaatta
     3301 aatattactt taccttatat tacgaataat ggagcaaaca tgtttttggg tgatacatgt
     3361 atttatgaat gtccaattga ttctagtgaa ttaaagactt gtttaaatat attagtcgca
     3421 aacaaagttg cgttcctggc ttatacaaat caaaccattt attcagttgg atatcaagaa
     3481 gggctggatg cgtttaagaa tcgattgatt ggtaagtgcg aaatagttga aaatgcgaac
     3541 atagaagata ttgttagtga atcaatattt aaggttgtga tgattcatcc aaatatggag
     3601 aagataaaaa atcaattaaa ttctgtttgt gacaaaacat tatgtatgca atcggaaggt
     3661 attatttata ctttaacaaa taaggattcg acgaagggta aaaccttgct aaagctttta
     3721 aaatatcttg atatggagcc aagttctgta ttagcttttg gggataacta taatgatatt
     3781 tctatgtttg aagttgcaga tggtgttgct gtagaaaatg cacaggagag tttaaaagaa
     3841 gtcgcaaagt atttaacaaa atcaaatgaa gatgatggtg tttcttattt tatccagaat
     3901 aaattgtgat agatgaattt ctatcacttt tttattgact ttggaaaagt aaactcctat
     3961 aatgcctata aagttactag gaataataag tggggtacta attatgaaac gatgttgtag
     4021 ctctttaaat gaataatatt gaatttgttt aataggagga aaagaaaatg acagtatata
     4081 aagcaatttc agatttaatt ggaaatacac cattagtaga attaacacat attgaagaaa
     4141 aagaaggatt agatgctagt gttgttgcga aagtagaatt ctttaatcca gctggaagtg
     4201 taaaggatcg catcgcaaag aaaatgattg aagatgctga gaaaaaaggg ttgattaagc
     4261 caggagctac attgattgag ccaacaagtg gaaatacagg tattggtatt gcgtctgttg
     4321 cagcagctaa gggatataaa gctattatga caatgcctga aacaatgagt gtagagcgtc
     4381 gtaacttatt gaaggcgtat ggcgcaaaag ttgttttaac agatggcaaa gcaggtatga
     4441 agggggctat cgcaaaagct caggaattgg cagctgctac accaaacagt tttattccaa
     4501 gtcaatttga aaatccatca aatcctcagg cccattatga atcaacaggt ccagaaattt
     4561 ggaaggatac agaggggaaa gttgatattt ttgttgccgg tgttggaacg ggtggtactg
     4621 taagtggcac aggtaaatat ttgaaggatc aaaatccaaa tgtgaaagtg gttgcggttg
     4681 aacctgcaac aagtccagta ctttcacaag gtcatgcagg tcctcatggt attcaaggta
     4741 ttggtgctgg ttttgttccg aatactttgg atacaagtgt atatgatgaa gtctttacag
     4801 tcacaaatga acaggcttat gaaacaggtc gtttaattgc acataatgaa ggtatgttag
     4861 tgggtatttc aagtggtgct gctacatatg ccgctattca aatcgcaaaa cgtcccgaaa
     4921 acaaagggaa aacaatcgtc gtattgttac cagatacagg tgagcgttat ttatctactc
     4981 cgatgtttgc ggaataataa aaaaatacga agtaaaaaat tacttcgtaa aagtcttggc
     5041 atagccatga tcttctctag aacccgtacc aatgggttct tttatttttc caatttcttc
     5101 aatttcaaat ggcgtcaatt gatatacata gtttagccag ttgcgatata gaagattggc
     5161 atggcttctc cattttaaga ttggttcttt ttctggatca tcatcgggaa aataattgaa
     5221 tggaatatgg atgggtttat tggcttttaa atctctaaaa tactcatctt ttaatgtttc
     5281 ttttccatat tctaaatggc caagaataaa gatttgacga tagtcctttg tgacaatgat
     5341 gtttgatcca atttcatcgt ttttagatag tatggataag catttcactt ttttgacggc
     5401 atcttcatcg attgccgtat gtctagagtg aggtgtatag tggatttcat caaagccatt
     5461 cgttaaaaag tcatattcat cgactaattg ttctgggaag atgccaaata ctttttctgg
     5521 caagatatgc ttttgaactc cataatgata ataaagtcca gcttgtgctc cccaacaaat
     5581 atgtaatgta gaatagacgt gagtcttaga ccattccatg atttcttcaa gttcattcca
     5641 ataatcaaca tcttcatatt caagatgttc tactggagct cctgtaatga tcattccatc
     5701 gtaataatta ttttttaatt tttcaaagta tgtgtagaat ttaactaaat gatcttggct
     5761 tgtattttta gaaacatgat ttttgaccat cataaaatca atatcaactt gtagtgcact
     5821 ttttgaaatt aaacgtaaga tttgtgtttc cgtttctatt tttttgggca ttagatttaa
     5881 aatcacgatc tttaatggtc gaattctttg tctatgtgca cttttatctt caatcgcaaa
     5941 gattttttca ctttctaata tgtcttttgc gggcaatgca tcgggtatgt tgattggcat
     6001 atataaacaa ctcctttttt tgtatacact ttattatact cgaaaaaaaa cagaatggaa
     6061 aattccattc tgtaaaagac tatgctttca ttgcgtctag tgcttgctgt aaatcgttga
     6121 taatgtcttg tgcatcttca agacctacag agaaacgaat taaatctgga gcaacaccag
     6181 cttcttttaa ttgttcttca ctcatttgac ggtgtgtatg acttgctggg tgtaatagac
     6241 aagttttggc gtcggcaaca tgggttacaa tagaagcaag ttttaagctg tccatgaatt
     6301 tttcggtttg atcacgatcg gcttttaatc caaatgacaa tacaccacaa gttccgtttg
     6361 gacaatattt tttagctaat tcatgatgag ggtcactttc aagatctgca tattttaccc
     6421 atgcaacatc ttcattagct tctaagtatt tagctacagc tagtgcattt gcacaatgtg
     6481 ccttcatacg aacggctaat gattctaatc ctaagttaag atagtatgca ttttgaggtg
     6541 cttggattga tcctaaatca cgcatcaatt gacttgtagc ttttgtgata taagctaatt
     6601 taccaaattt ttcagcatag acaattccat gatacgattc atcgggtgta gttagtcctg
     6661 gatatttatc ggcgtgttct agccagttga aattaccact atctacaata gctccaccta
     6721 cacaagatgc atgtccatcc atatattttg tggtagagtg agtaacaata tcagctcccc
     6781 attggattgg ttgacacata attggtgtag cgaatgtgtt atcgataatt aatggaactc
     6841 cattcgcatg tgcaactttc gcaaattttt caatatcaaa aatacgtaca gttggatttg
     6901 tgattgtttc tccaaagaca cattttgtat ttggcttgaa ttctttttga agatcttctt
     6961 cactgatttc tgggtcaata aatgtacaat caatacccat tttcttcatt gttacaccaa
     7021 ataggttgta tgttccacca tagatagcac ttgaagcaat taggtgatct ccagcttcgc
     7081 agatgttgaa taccgcaaag aagttggcag cttgtccact tgatgtaagc atagcagcaa
     7141 taccaccttc taatgcagca attttactag cgacaatatc atttgttggg ttttgaaggc
     7201 gtgaatagaa atatccactg tcttctaagt caaataatct tcccatttga agacttgaat
     7261 catatttaaa agttgtactt tgataaattg gaacttctct tggctcacca ttttttggct
     7321 cgtatccggc atgtaaacat tttgtttcag ttttcataat aaatttacct catttcctaa
     7381 ccattattgt aatattatct ttaaaaaaag taaaagaaaa gcttagatta tataatcaaa
     7441 gctttgttca cgatttaaaa tgtcttgtag tgagattcct tttaaatagg t
//
LOCUS       DBMDWL010000103         7465 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3685_length_7465_cov_2.590847, whole genome shotgun sequence.
ACCESSION   DBMDWL010000103 DBMDWL010000000
VERSION     DBMDWL010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3685_length_7465_cov_2.590847"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="ACZPDN_07685"
     CDS             complement(<1..175)
                     /locus_tag="ACZPDN_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR1298542.1"
                     /translation="MIRIGVYGLGKISYRVIQGILYANNADLYAVCSSNLEKANAFKE
                     EYRARKAYDSYEEM"
     gene            complement(312..1451)
                     /locus_tag="ACZPDN_07690"
     CDS             complement(312..1451)
                     /locus_tag="ACZPDN_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1298543.1"
                     /translation="MEKEFKAIYGQAMSIVTHKPSQYAKNITLRYPMKSTFKGDKVRI
                     CLDNFTGKEGVVFDSVTIGRVISGRDLDPTSIVDITFNGSKGLHLDVGEQCYSDPLDF
                     KVHEQETIAISFYLKEFTHLRCGVYTQGPLSTGYFGEGDFSHAGIMDHASSMKTPWVF
                     FLSEVAVLTDAKNQVITCYGDSITSQDWPDYLQKLMWENNKKVSVVRKAVSGTRILRQ
                     YSCIQYEAYGLKGDYRFGHEMHVAGSDTLIILQGINDIIHPVGVEKNEFRPWQDLPSS
                     QELIEGLRKYVKHAKSLGMKVYIGTLIPIEGWRTYAYFRENLRNEVNEWIRTTDEIDG
                     VIDFDKDICDGHKMQDAFDSGDHLHPSDEGYAQMAKTAYNKMSME"
     gene            1589..2353
                     /locus_tag="ACZPDN_07695"
     CDS             1589..2353
                     /locus_tag="ACZPDN_07695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1298544.1"
                     /translation="MSKLENYYGKFCEDKRLLSRHGQVEYTTSMKYIHKHLKSEDRIL
                     DIGAGTGRYSVALSNEGYQVDAIELVKYNLGVLKAKKSNVKAYQGTALDLSRYQDNTF
                     DVTLLFGPMYHLFSYEDKLKALSEAKRVTKKDGIIFVAYVMNEYSVLVHGFRDGHIKE
                     SLEAKKLDETFQTQTNIDDLYSYIRLDTINQLNEDVGLERIQIIAADGPSDYMRPILN
                     KMDDETFELFIKYHLATCERQELIGASSHTIDILRK"
     gene            complement(2376..2678)
                     /locus_tag="ACZPDN_07700"
     CDS             complement(2376..2678)
                     /locus_tag="ACZPDN_07700"
                     /inference="COORDINATES: protein motif:HMM:NF017273.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR1298545.1"
                     /translation="MSNPMMYVATMAVVTYLIRVLPLTLFNKPITNIYIQSFLYYVPY
                     VTLAVMTFPTILYETNSQLAGLAALMIGIVLAWKDKSLFQVSFACCFVVLTMEYII"
     gene            complement(2671..3357)
                     /locus_tag="ACZPDN_07705"
     CDS             complement(2671..3357)
                     /locus_tag="ACZPDN_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015549.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1298546.1"
                     /translation="MEFKKGIKDSIPVGMGYFAVSFSLGIIAKNVGLSPIQGFLTSLF
                     INASAGEYAAFWMISTHGTFIQMIIMTLVSNARYFLMSFALSQKIEEKTTLFHRMCIG
                     FDLTDELFGLAINHKGKVNYKYIYGTYLVALPCWAFGTALGIIAGNSLPTKIVASLSV
                     ALYGMFLAIIIPQAKKDRIVGVLVVVSFLLSYICKDIPISSGTRTILLTIIISMVAAI
                     IKPRKKEENV"
     gene            3450..4355
                     /locus_tag="ACZPDN_07710"
     CDS             3450..4355
                     /locus_tag="ACZPDN_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1298547.1"
                     /translation="MNLTKYDVFFKVLETKTISQAAKELGYSQSAVSQTIKSLEEELE
                     ATLFIRQKSGVILSKDGHAYLPYLKSVQASLANLRTKKQEMKGLDQAILRIGTFTSVS
                     RHLLPSLMKEFIEMYPNVQFELYQSEYTNIEQQLLEGSLDLGFTCIDAIQQVQYQELY
                     YDEMFALVPQNHVLANYEVLSMEQIAKYPFIQLDEGEYSLPIKTFQNLGLHVQIKHKV
                     YDDFTILAMIRQNLGISILYQNALSDYDDLCLIPIQENLFRHVGIAYNNYDTLSYAGK
                     TFLDFILRKTPEILKNKDSFHIIQI"
     regulatory      4419..4585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            4706..6046
                     /gene="mgtE"
                     /locus_tag="ACZPDN_07715"
     CDS             4706..6046
                     /gene="mgtE"
                     /locus_tag="ACZPDN_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR1298548.1"
                     /translation="MMNKEEFIQLLQDKKYKTAQNILSEMNDMDTAAFLEELDAKECA
                     LAFRLISKEKAADVFSCMNSKMQTFLVDMFSETELKELLDDLYMDDTVNMLEDLPANL
                     VTRILKNLDSTKRSTINKLLKYPEDSAGSIMTTEYVTLKKAMTVKQAMNHIKEIGIHK
                     ETIYTCYVTENKRLMGIVTAKILMTSDDDENITNLMNTNIISVHTHTDQEQVAHLFRK
                     YDLIAMPVLDSDGCLVGIVTFDDAMDVMVDETNEDISRMAAMEPNEKTYFETTVWQQA
                     KHRILWLLVLMFSATITGSIITRYENAFSAVPLLVSFIPMLMDTGGNCGSQSSTLIIR
                     GLALGEIHFKEIFKVMFKEFRISLIVGSILAVANGLRIFIQYQNFNIAIVVALSLMVV
                     VIIAKFIGCILPLFANKFGVDPAIMASPLITTIVDTCSILVYFNIAVHIFNIVV"
     gene            complement(6135..7106)
                     /gene="pta"
                     /locus_tag="ACZPDN_07720"
     CDS             complement(6135..7106)
                     /gene="pta"
                     /locus_tag="ACZPDN_07720"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR1298549.1"
                     /translation="MIIDELEKKVQGKGVRIVFTEGWDPRVQKAAIDLKNNDVVCPVL
                     LGTPEEIAGCAQKNSLNVEGIEQIDPNNFEHMDEMVRKMVELRRGKMDEEKVRTALKS
                     TNYFGTMLVQMGYADGLLGGATYSTADTVRPALQLVKAAPGNKLVSSCFILIKEETQL
                     IMGDCSININPNTDDLVEITMQTAKSARQFGVEPKVALLSYSTMGSAKGECVAKMTEA
                     SDRLRRIADFDIDGEMQFDCAVSEEVAKLKAPNSKVAGHANTFIFPNLSSGNIGYKIA
                     ARLGGYEAVGPILQGLNAPINDLSRGCTSDEIYKMAIVTAAQKDMDI"
BASE COUNT         2484 a         1329 c         1163 g         2489 t
ORIGIN      
        1 acatctcttc atagctatcg taagcctttc ttgccctata ctcttcttta aaagcatttg
       61 ccttttctaa gtttgaagaa cacacggcat ataaatctgc attattcgca tatagaattc
      121 cttgaatcac acgataacta atttttccaa gtccatagac tcctattcga atcatttttc
      181 attcaccctc tttaagatta ctttaagcta tattgcttat tctatatata gaaataagca
      241 gtcaacctct tttgctcact atacttatct cttctgccta tttctttcat atatgacaaa
      301 cgtccatgga actattccat ggacatctta ttgtatgcag tttttgccat ttgcgcatat
      361 ccttcatcac ttggatgtaa atgatctcca ctatcaaatg catcttgcat tttatgtcca
      421 tcgcaaatat ctttatcaaa atcaattacg ccatcaattt catccgttgt acgaatccat
      481 tcattgactt cattacgcaa gttctctcta aaatatgcat aagtgcgcca accttcaatc
      541 ggaatcaacg tcccaatata gaccttcatt cctaatgact tcgcgtgctt tacatatttt
      601 cttaagccct caatcaattc ctgacttgac ggaagatctt gccatggtct aaattcattc
      661 ttttcaacac cgactggatg aataatatcg ttgattcctt gcaaaataat taaagtatca
      721 cttccagcca catgcatttc gtgtccaaag cgataatcac cttttaatcc ataagcttca
      781 tattgaatac aagaatactg tcttaaaata cgtgttccac taacggcttt tcgaactaca
      841 gacacttttt tattattttc ccacatgagc ttctgtaaat aatccggcca atcctgtgaa
      901 gtaatagaat caccataaca tgtgataact tggtttttgg catcggtaag taccgcaact
      961 tcacttaaga aaaataccca aggtgttttc atacttgaag catgatccat aatgcctgca
     1021 tgagaaaaat cgccttcccc aaagtagcct gtgcttaatg gtccttgtgt atagacgcca
     1081 caacgtaaat gcgtaaattc ttttaggtaa aaacttatcg caatggtttc ttgttcatgc
     1141 accttaaaat ccaatggatc actataacat tgttctccca catcaagatg aagtcctttt
     1201 gaaccattaa aagtaatatc aacaatggat gttggatcca aatcacgccc actaatcact
     1261 ctacctattg tgactgaatc aaagaccaca ccttctttac ctgtaaagtt atccaaacaa
     1321 atacgtactt tatccccttt gaatgtcgat ttcataggat aacgcaatgt aatattttta
     1381 gcatattgac taggtttatg cgttacaatt gacattgcct gtccatatat cgctttaaat
     1441 tctttttcca tagttgacct ccaaattatt tattatctat ttctacattc ataaaacata
     1501 tgtttttatc cacaaataat catacatgaa acgttgcaaa aatccaaaca tataccttat
     1561 tatattaaag aagttgagga aataatgcat gagtaaatta gaaaattatt acggtaaatt
     1621 ttgtgaagat aaaagactct tatccagaca tggccaagtt gaatatacaa caagcatgaa
     1681 atatatccat aaacatctaa agtctgaaga tcgcatactc gatattggag ccggaactgg
     1741 cagatatagt gttgccttaa gcaacgaagg atatcaagtc gatgcgattg aacttgttaa
     1801 atataatctt ggtgttttaa aagctaaaaa aagcaatgtg aaggcctatc aaggcactgc
     1861 tttagattta tcaagatatc aagataacac ttttgacgtc accctactat ttggacctat
     1921 gtaccactta tttagttatg aagataaatt aaaagcatta tccgaagcta aacgtgttac
     1981 aaaaaaagat ggaattatat ttgtggctta cgttatgaat gaatatagcg ttctagttca
     2041 cggctttaga gatgggcata ttaaagaatc attagaagct aaaaaactag atgaaacatt
     2101 tcaaacccaa acaaatattg atgatttata cagttacatt cgtctagata caattaatca
     2161 attaaatgaa gatgtaggtt tagaacgaat ccaaattatt gcagccgatg gcccaagtga
     2221 ttatatgcgt cctatcttaa acaagatgga tgatgaaaca tttgaattat tcatcaaata
     2281 tcatcttgcg acatgtgaaa ggcaagaact aattggtgct agctcacata caatagatat
     2341 attaagaaaa taatgtcact tttgtggcat tattttcata ttatatattc cattgttaaa
     2401 actacgaaac agcatgcaaa cgatacttga aataagcttt tatctttcca tgccaaaaca
     2461 attccaatca taagtgctgc tagtcctgca agttgtgaat ttgtttcata caatatagta
     2521 ggaaaggtca tcaccgccaa agtcacataa ggaacataat atagaaatga ctgaatgtat
     2581 atattggtaa tgggtttgtt aaaaagtgtt agtggcaata cacgaattaa atatgtgaca
     2641 actgccattg tagctacata catcattgga ttagacattt tcttccttct ttctaggttt
     2701 aataatggcc gctaccattg aaataatgat tgttaataaa atagttcttg tcccacttga
     2761 gattggtata tctttacaaa tgtagctcaa taaaaagctc acaacaacaa gaacacctac
     2821 aattcgatct ttctttgcct gaggaataat aatagctaaa aacatgccgt acagtgcaac
     2881 gcttaaactt gctacaattt ttgtaggaag actatttccg gcaatgattc ccaatgccgt
     2941 tccaaatgcc caacaaggaa gtgccactaa ataggtacca tatatatatt tataattcac
     3001 ttttccttta tggttgattg ccaaaccaaa caattcatct gttaaatcaa agccaataca
     3061 cattctatga aacaaagttg tcttttcttc aatcttttgg cttagcgcaa agctcattaa
     3121 gaaatagcgt gcattactga ctaaagtcat gataatcatc tgaatgaaag taccatgtgt
     3181 actaatcatc caaaaagccg catattctcc tgctgatgca ttgataaata aactagtcaa
     3241 aaagccttgt attggtgata ggcctacatt ttttgcgatg attcccaacg aaaaggaaac
     3301 cgcaaaatat cccattccta ctggaatact atctttgatt ccttttttaa actccataat
     3361 ctcactcctt atttctatgc attataagaa tcgaattgtt atttgtaaat caaatattta
     3421 taatgtatcc ataaggaaat atgatagata tgaatctaac aaaatacgat gtttttttta
     3481 aagtattgga aacaaaaaca attagtcaag ctgctaaaga acttggctat tcacaaagtg
     3541 cagtttctca aacaataaaa tctttagaag aagagctaga agccacttta ttcattcggc
     3601 aaaaaagtgg tgtcattcta agcaaagatg ggcatgcata tcttccctat ttaaaatcag
     3661 ttcaggcaag tcttgctaat cttcgtacta aaaagcagga aatgaaagga ttggaccagg
     3721 ccatattaag aattggtaca tttactagtg tatctcgaca tcttttgcct tcattaatga
     3781 aagaattcat tgaaatgtat ccaaatgtac aatttgaact gtatcaaagt gaatatacaa
     3841 atattgaaca acaattatta gaaggaagtc ttgatttggg attcacttgt attgatgcca
     3901 tccaacaagt tcaatatcaa gaattgtatt atgatgaaat gtttgcactt gtccctcaaa
     3961 atcatgtgtt agctaattat gaagttttaa gcatggaaca aatcgcaaaa tatcctttca
     4021 ttcaattaga tgagggagaa tattctttac cgataaaaac atttcaaaat ctaggattac
     4081 atgtacagat taaacacaaa gtatatgatg actttactat tttagctatg atacgacaga
     4141 atttaggtat ttccatactt tatcaaaacg ctttgagtga ctatgatgat ttgtgtttga
     4201 ttccaataca agaaaattta tttcgtcatg ttggaatcgc ctataataat tacgatactt
     4261 tgtcttatgc aggtaagaca tttctagatt tcattcttag aaaaacacca gaaattctta
     4321 aaaataaaga tagctttcat attattcaaa tttaacatag tttttatact gttaaggttg
     4381 actcacttac taatttatat tattcttttc acgtacataa agatatacgg taggtgaggt
     4441 taccgcaggg atttggattt cctaagatta ctgccgcaaa gttgtggaga cacgactcgt
     4501 tggtcagcag gcaaagtcgc aaaggcttgg cttaatgtaa tttatatgcc ctgtgatact
     4561 aaagcttgaa cggtgatact agattaataa cgtcattgtt taagcaatgg cgttttcttt
     4621 tgttgataag gaggtgaggc ttgtatgtgt acggatccgg atgggaatag tttgaattat
     4681 aacaagaaag cagaggtaca taatcatgat gaacaaagaa gaattcattc aattactaca
     4741 agacaaaaaa tacaaaactg cacaaaatat actttccgaa atgaacgata tggatacagc
     4801 tgccttttta gaagaattag atgcgaaaga atgtgctttg gcatttcgct taatttctaa
     4861 agaaaaagct gccgatgtat tctcatgcat gaattcaaaa atgcagacat ttttggtaga
     4921 catgttttcg gaaactgaat taaaagaatt attagacgat ttatatatgg atgatacggt
     4981 caatatgtta gaggatctac ccgccaactt agttacgcgt attttaaaga acttggattc
     5041 aacaaaaaga tcaacaatta ataagctttt aaaatatccg gaagacagtg ccggttccat
     5101 catgacaact gaatatgtca ctttaaaaaa ggcaatgaca gtaaaacagg ccatgaacca
     5161 tattaaagaa attggtattc ataaagaaac tatctatact tgttatgtca ctgaaaataa
     5221 aagattgatg ggtattgtca ctgccaaaat tttaatgaca agtgatgatg atgaaaacat
     5281 cacaaatttg atgaacacaa atatcatttc tgtgcatacg catacagatc aagagcaagt
     5341 agctcactta tttagaaaat atgatttaat tgcgatgccc gtacttgata gtgatggatg
     5401 cttagttggt attgtcacat ttgatgatgc catggacgtt atggttgatg aaactaacga
     5461 agatatttcg cgtatggctg ccatggagcc caatgaaaaa acatattttg aaactacggt
     5521 ttggcagcag gcaaagcacc gtattttatg gttacttgtt ttgatgtttt ctgcaacaat
     5581 caccggaagc attattactc gatatgaaaa tgcctttagt gctgtaccat tacttgtgtc
     5641 ctttattcct atgttgatgg atacgggtgg aaactgtggt tctcaaagtt ccactttgat
     5701 tattcgtggt ttggctttgg gggaaattca ctttaaagaa atttttaaag ttatgtttaa
     5761 agaatttaga atttcattaa ttgtcggatc tattttggct gtagctaatg gcttgcgtat
     5821 tttcattcag taccaaaact tcaatatcgc aatcgttgtt gctctttcat taatggttgt
     5881 cgttattatt gccaaattca ttggttgtat tcttccatta tttgcgaata aatttggtgt
     5941 agatcctgcc attatggcaa gtccattgat tactacaatc gtggatactt gctctattct
     6001 agtttatttc aatattgcgg ttcatatttt taatatcgta gtttaagagg tgagaaatca
     6061 tctctttttt actaaatacg aaaaaaaaag aacctcaatt gagattcttt tatcaaaaaa
     6121 tataattatt taaattagat atccatatct ttttgagcag cagttacaat agccatcttg
     6181 tagatttcat ctgaagtaca tccacgagac aagtcgttga ttggagcgtt caatccttgt
     6241 aagattggtc caactgcttc atatccacct aaacgagcgg caattttgta tccaatattt
     6301 ccacttgaca agttagggaa gataaatgta ttcgcgtgtc cagcaacttt tgaattagga
     6361 gcttttaatt tagcaacttc ttcacttact gcacaatcaa attgcatttc tccatcgata
     6421 tcgaaatcag caatacgacg taaacgatcg cttgcttcag tcatcttagc aacacattca
     6481 ccttttgcac tacccattgt tgaatatgat aataatgcaa cttttggttc tacaccaaat
     6541 tgacgagctg acttagctgt ttgcattgtg atttctacta aatcatctgt gttagggttg
     6601 atgttaattg agcagtcccc catgatcaat tgagtttctt ctttaattaa aatgaagcaa
     6661 gaagaaacta atttgtttcc tggagctgct tttactaatt gtaatgctgg acgtacagta
     6721 tccgctgtag aatatgtagc accacctaat aatccatcag cgtatcccat ttgaactaac
     6781 atagttccaa aatagtttgt gctcttcaat gctgtacgaa ctttttcttc gtccatttta
     6841 ccacgacgta actcaaccat tttacgaacc atttcatcca tatgttcgaa gttatttgga
     6901 tcgatttgtt cgattccttc aacatttaaa ctattttttt gtgcacagcc agcaatttct
     6961 tctggagttc ctaataatac tgggcaaaca acatcattgt ttttcaaatc aatagctgcc
     7021 ttttgtacac gtggatccca tccttcagtg aatacaatac gtactccctt tccttggacc
     7081 tttttttcta attcatcaat aatcataact ttaactacct cttttctatc aaagtatagt
     7141 ccaaaaatgc tttatttacc acaaaaacaa tgtgaacttt ttctcatagt ttatttataa
     7201 aatgtgtatg tatttaataa catgttgttc gtatgttatg tacattatgt ataaaatgta
     7261 attattttac atacaattga aaattaatgt aaatttatct tgcatttctt tctgtaaagc
     7321 tttacactaa tgacatcaca agaagggata ggtgcaaatt atgaaaacaa gtcactattt
     7381 aaccttaact ttgcagaatt tgtataacta aaaaataaaa gaagcattag tagctgaatt
     7441 caccaatgct tctgtcaata tattt
//
LOCUS       DBMDWL010000104         7377 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3748_length_7377_cov_2.630979, whole genome shotgun sequence.
ACCESSION   DBMDWL010000104 DBMDWL010000000
VERSION     DBMDWL010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3748_length_7377_cov_2.630979"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      145..242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            312..863
                     /locus_tag="ACZPDN_07725"
     CDS             312..863
                     /locus_tag="ACZPDN_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIR1298550.1"
                     /translation="MNQYFVMLKENARSLKNVRVIVGVALFFALNVILNLFGSIQITN
                     QLKLGFASIATAASCLLYGPVPNLVLAPLLDLVNYMVKPVGAYYPIFMISTMASACIF
                     SAYFYNCEKITWVRVILCRITYDVVVSLFLNTLFTSMLWSTPFLVIAGPKLIKNLISL
                     PFQVVILYLVMKTCIQLKPKVNR"
     gene            complement(878..1495)
                     /locus_tag="ACZPDN_07730"
     CDS             complement(878..1495)
                     /locus_tag="ACZPDN_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013756.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HIR1298551.1"
                     /translation="MDNTVLYDLSYGMYAVGVKDGMRECGCIVNTVFQVSTIGPLIAL
                     SMNKDNYTCSLIEKSKCFSLSILPETIDSQVITDLGFQTGKDKDKWAKLNHHLFRELP
                     VVDDALGYMMCEVQSQMDAGTHFVFLAKVVDAKKGDSGKPMTYAYYHNVLKQSAPAKA
                     PTYRKEEKWVCSVCGYVYDGDDFENEPDDYVCPVCGAKKEMFKLQ"
     gene            complement(1554..1985)
                     /locus_tag="ACZPDN_07735"
     CDS             complement(1554..1985)
                     /locus_tag="ACZPDN_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1298552.1"
                     /translation="MFTIEEFLRISNQMRDYYAKKIKDSFQEFNFSPNEISILIILKN
                     NSTITTSTELKVVLGVSKTLISRSVDSLEKKGLIRICIDGKDTRIHHLRLTDRCQPVL
                     KIIDEEIGKINKTLFYDVSVEEMKSLKQTMNKLQKRVEEGK"
     gene            complement(2037..3197)
                     /locus_tag="ACZPDN_07740"
     CDS             complement(2037..3197)
                     /locus_tag="ACZPDN_07740"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1298553.1"
                     /translation="MNIQEKINKMENCRGKNHDLLEFRQWLTDAMPNHTALKKIQVAG
                     TNGKGSTSIWLRDLLMKKGYRVGVFTSPHLIHHTERIRVNDTCISLADWERIYDQYEE
                     LFSFHKMTMFEMDLWMSLAYFIEQQVDYCIIEAGMGGRLDATTALDYQACLVTNVGLD
                     HTEYLGNTFEQIAYEKSGIFKPNVPALTTEDKPECQKVMELVADYMNTPLCFIDCQET
                     DVLRWNGMTFHLNPPLYQAKNLSLALETLNVLGIWLEENEIQEVIDHFQWAGRFMVLR
                     KAPLVLLDGAHNVHGITALTHSLKDFKGKIYFSVLKEKDAPHMIELLQTISKDITLVE
                     ISSARLYPLKKLGYPIITVDELISILLDAKEDSLLCGSLYYVGEVLEKWGNV"
     gene            3234..3893
                     /locus_tag="ACZPDN_07745"
     CDS             3234..3893
                     /locus_tag="ACZPDN_07745"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1298554.1"
                     /translation="MIMHNDWDQLFDQEIQKDYLQNLRYFLAKEYKTKNIYPAKENIF
                     AAFKTTSLKDTKVVILGQDPYHGPGQAQGYSFSVPSNFPLPPSLQNMYKELENEYQTP
                     VHRTGDLTDWAKQGVLLMNTILTVEEHKPLSHQDMGWQNFTNEALKWINEKDGPVVFL
                     LWGAKAIQAKKLLTNPKHLILTSPHPSPLSAYRGFFGNNHFKLANEYLVKNNETPIQW
                     I"
     gene            complement(3943..4797)
                     /locus_tag="ACZPDN_07750"
     CDS             complement(3943..4797)
                     /locus_tag="ACZPDN_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1298555.1"
                     /translation="MKIAFVTDTGTGQHPDYWKEKGIYCLPLQITCDGESKDEYTEIT
                     YSEVIQKLHEKKVLKTSLPSLGKIQDCFEKLKHEGYDTIFAVPICRGLSGTLDSMEMI
                     ANQLEMKFVGVDCYCTAVEQGCMIETAKKMYEANIPIDEIIKKLQSIADSCETVLLCD
                     DLDHMKRGGRLTPMAAALGGLLKIKPILHIGKSTSGKVDVLDKVRTMKRAQDRVIKHL
                     KELNVDSNYTFIVAHVDALEAAKEYAQKIESQIEGAKVKVIDLVSAVGIHTGLGCLAL
                     QVFNEMKD"
     gene            complement(4800..5660)
                     /locus_tag="ACZPDN_07755"
     CDS             complement(4800..5660)
                     /locus_tag="ACZPDN_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1298556.1"
                     /translation="MKIRVLTESGCGLTKQEANDLGIDFLPLQVQIEDKTYLDGVDLT
                     VDELNSFLERGFMPQTSMPPLGDIEALLESYKKDGITDVILITLSNGLSGTNQAIQAS
                     GKWHGVKIHTLDIYTTLGVEKYLVLSAQKLVEQKIHPEEIISRLKESVEHSRGFLIPE
                     DLDHLAKGGRLTPMAAKLAGMLKIKPILEVSKNTEGKVDVFDKVRTMSKAIKKAVKVI
                     SKDANAQDYEFYVLNGENQEGTQLVIDELHDVFGENIQIHTDPMYAVIAVHTGLGAVG
                     VQFVKKVKGA"
     gene            5751..6455
                     /locus_tag="ACZPDN_07760"
     CDS             5751..6455
                     /locus_tag="ACZPDN_07760"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298557.1"
                     /translation="MSAFISFENVKKTYHMGEIDIHALDDVSFEIEQGEFVIIAGASG
                     AGKSTILNLLGGMDTVSEGKIIVDGREISSYKEKDMTLYRRYDVGFVFQFYNLVQNLT
                     LRENVELATQICKKPLDIDETIDLVGLKDRTYNFPSQLSGGEQQRVAIARALAKNPKL
                     LLCDEPTGALDYQTGKQVLKVLQDTCKTQNKTVIVITHNLALTPMGDKVIKVRSGKIE
                     SITYNEHPMDVNDIEY"
     gene            6457..>7377
                     /locus_tag="ACZPDN_07765"
     CDS             6457..>7377
                     /locus_tag="ACZPDN_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1298558.1"
                     /translation="MPATYLKDIFREIKISLGRFLSILCIVAIGVAFFAGIKASAPDM
                     KNSADTYFDKYNVQDIQVYSTIGLTKKDVAAIKKIKGVKSVQPSFSMDTLSQIDSTQM
                     VIKVISYGIDQKMNKIRVVEGRMPERENECLVEASSATNKLYGTFHIGDTIKLQSGTD
                     EALSKSLKHTKYKIVGTCYNPNYLSYEKGSSNIGSGTVNSFIYIQNTNVLKDYYTEVD
                     VCVKGAKDLDCYSDEYFDVVDPVLKKIKKISNKQIDARIQSYQSELDEKKQEATDKFK
                     DAENQFNDAQNKIDSGLSEIQSNELKLQNSK"
BASE COUNT         2399 a         1341 c         1088 g         2549 t
ORIGIN      
        1 agtgtacacc aatacactat taaagtatat aaaaaaataa ataatcttca aacatgaaaa
       61 attgaagatt atttcatgcg taatcactaa caattaaaga gatctgttga cagatctctt
      121 tttaatacat ataattcaac tacatgcaga gtaaaaatat gtgcgttaag tgtctttaac
      181 acggggagtt ggttaaggaa cgaaaagtta ttcttgcggt acatattttg catcccgctg
      241 ttgtgatcga aaaaaattaa cgactttctt tttttgtatc gcaatatgga aaaaaggagg
      301 tctttttata tatgaatcaa tactttgtaa tgttaaaaga aaatgcacgt tccctaaaaa
      361 atgttcgtgt cattgttggg gtagctttat tttttgccct aaatgttatt cttaacttgt
      421 ttggatccat ccaaatcaca aaccagctaa aattaggctt tgcatccatt gcgacagctg
      481 ctagctgctt actttatgga cccgttccaa acttagtgct tgcaccatta ttagatttag
      541 tcaattacat ggttaaacct gtgggtgcat attatccaat ctttatgatt tctacaatgg
      601 caagtgcatg tattttttca gcttacttct ataactgcga aaaaatcact tgggttcgcg
      661 tcatcttatg tcgaataacc tatgatgtag ttgtcagttt attcttaaac acactgttta
      721 catctatgct gtggtcaacg ccatttctag ttattgctgg ccctaaatta atcaaaaact
      781 taatttcatt accattccaa gttgttattc tatatttagt gatgaaaaca tgtatccagt
      841 taaaaccaaa ggtgaaccgt taatcggtcc acctttttta ctgcaattta aacatctctt
      901 ttttggcccc acaaactgga catacataat catctggttc attttcaaag tcatctccat
      961 catatacata tccgcaaaca ctacataccc acttttcttc ttttctgtat gtaggtgctt
     1021 ttgccggtgc gctttgtttt aagacattat gataatacgc ataggtcatt ggtttaccag
     1081 aatcaccttt cttggcatca acgactttgg ccaagaatac aaagtgtgtt cctgcatcca
     1141 tctgactttg tacttcacac atcatgtatc ctaacgcatc atctacaact ggcaattccc
     1201 taaacaaatg atgattcaac ttcgcccact tatctttatc ttttcctgtt tgaaagccta
     1261 aatccgtaat cacttgtgaa tctattgttt caggcaagat tgataaagaa aaacacttac
     1321 ttttttcaat caatgaacaa gtataattat ccttattcat actcaaggca atgagtggtc
     1381 ctatcgttga aacttgaaat acagtattca caatacaacc acattcgcgc atgccatctt
     1441 ttacacccac ggcatacata ccataagaaa gatcatacaa aactgtgttg tccataagaa
     1501 cacctccctt gttactttaa ttatagcaag tttagtggtt atatactata caactattta
     1561 ccttcttcaa cacgtttttg taatttgttc attgtctgct tcaaactttt catttcttct
     1621 acagacacat cataaaataa tgttttatta atttttccaa tctcttcatc aataatcttt
     1681 aaaacaggtt gacacctatc tgttagtcgt aaatgatgta ttcgggtatc ttttccatct
     1741 atacaaatac gaatgagccc ttttttctcc aaagaatcaa cacttctaga aatcaaagtt
     1801 tttgaaacgc ctaatacaac ttttaattct gtactcgttg taattgttga attatttttt
     1861 aagataatca aaatagaaat ttcatttggt gaaaaattaa actcttgaaa actatctttt
     1921 attttttttg cataataatc tctcatttga ttggatatac gcaaaaactc ctcaattgta
     1981 aacatattat cgctccaact ctaatagaac aattgtaagt gctaaaaagg ggcgtgtcaa
     2041 acattgcccc atttttctaa tacttctcct acataataga gagaaccaca taataaactg
     2101 tcttcttttg catctaaaag tattgaaatc aactcatcca ctgtaataat aggatatcct
     2161 aattttttaa gcggatataa acgtgcactt gaaatttcta ccaatgtaat atctttacta
     2221 atggtttgta gaagttcaat catgtgtggc gcatctttct ctttcaatac agagaaataa
     2281 atctttcctt taaaatcttt caaagaatgt gtcaacgcag taattccatg tacattatgt
     2341 gctccatcca acaaaaccaa tggagctttt ctaagaacca taaatcggcc tgcccattga
     2401 aaatggtcaa tgacttcttg tatttcattt tcttcaagcc aaatacctaa tacatttagt
     2461 gtttccaagg caagagataa atttttagct tgatacaatg gtggattcaa atgaaaggtc
     2521 attccattcc atctcaatac atctgtttct tggcaatcga taaaacatag tggcgtattc
     2581 atataatcgg caacaagttc cattactttt tgacattctg gtttatcttc cgttgtcaaa
     2641 gcaggaacat ttggtttaaa aatacctgat ttctcatatg caatctgttc aaaagtattc
     2701 cctaagtatt ctgtatgatc gagtccaaca ttggtaacca aacatgcttg ataatccaat
     2761 gccgttgtcg catctaaacg ccctcccatt cctgcttcaa taatgcagta atccacttgt
     2821 tgctctataa aataggcaag tgacatccat agatccattt caaacatagt cattttatgg
     2881 aatgagaata actcttcata ttgatcatag attctttccc aatcagctag tgaaatacat
     2941 gtatcattca cgcgtattct ttctgtatga tgaatcaaat gtggggatgt aaaaacacct
     3001 actctatatc cttttttcat caacaaatct cttaaccaaa ttgatgtact accctttcca
     3061 tttgttcctg caacctgaat tttttttaag gcagtatgat ttggcattgc atctgttaac
     3121 cattgtctaa attctaataa gtcgtgattt tttcctcgac aattttccat tttattaatt
     3181 ttctcttgta tattcatgat attattatat cactaaagaa agaagtgaat cacatgatta
     3241 tgcataatga ctgggatcaa ttgtttgatc aagaaataca aaaagattat ttacaaaatt
     3301 tacgttattt tttagctaag gaatataaaa caaaaaatat ttatcctgca aaagaaaata
     3361 tttttgcagc ttttaaaaca acttcattaa aagatacaaa agttgttatt ttaggacaag
     3421 atccatatca tggtccagga caagctcaag gttactcatt tagtgtacca agcaactttc
     3481 ctttacctcc atctttacaa aacatgtata aagaactaga aaatgagtat caaactcctg
     3541 tacatcgtac tggcgattta acggactggg ctaaacaagg agtactatta atgaatacca
     3601 ttcttacagt tgaagagcat aagcctttat ctcaccaaga tatgggatgg caaaatttta
     3661 cgaatgaagc tttaaaatgg attaatgaaa aggatggtcc tgttgttttc ttattgtggg
     3721 gtgctaaggc tatccaagcc aaaaaattat tgacaaatcc aaaacattta attcttacaa
     3781 gtccgcatcc aagtccttta agtgcttatc gaggtttctt tggaaacaat cattttaagc
     3841 ttgcaaatga atatttagtt aaaaacaatg aaacgcctat ccaatggata taggcgtttt
     3901 tcttatagtg caaaagaaaa agaagcatca ttcttgatac ttctagtctt tcatttcatt
     3961 aaacacttgt aatgctaagc agccaagtcc tgtatgaatt ccaactgcac taactaaatc
     4021 aatgactttg acttttgctc cttcaatttg actttcaatc ttttgtgcat attcttttgc
     4081 agcttctaac gcatcaacat gtgcaacaat aaatgtataa ttagaatcta cattcagttc
     4141 ttttaaatgt tttataacac gatcctgggc acgtttcatt gtacgtactt tatctaatac
     4201 atccactttt ccacttgtac ttttaccaat atgcaaaata ggtttgattt tcaataagcc
     4261 acctaatgca gcagccatcg gtgtcaatct tccaccacgt ttcatgtgat ctaaatcatc
     4321 acacaacaac acagtttcac aactatccgc aatgctttgt agttttttta taatttcatc
     4381 aattggaatg tttgcctcat acatcttttt agctgtctca atcatacatc cttgttcaac
     4441 cgctgtacaa taacagtcaa cgcctacaaa tttcatttct aattgattcg caatcatttc
     4501 catagaatcc aatgttccag ataatcctcg gcaaatagga accgcaaaaa tcgtgtcata
     4561 tccttcatgt tttaattttt caaaacaatc ttgaatttta cctaaactag gcaaggatgt
     4621 tttcaacact tttttctcat gtaatttttg aatcacttct gaatatgtaa tttcggtata
     4681 ttcatctttt gattcaccat cacatgtaat ttgtagaggt aagcaataaa ttcctttttc
     4741 tttccaatag tctggatgct ggcctgttcc ggtatccgtt acaaatgcaa tcttcatggc
     4801 taggccccct ttactttttt cacaaattga acgcctacag cacctaatcc tgtgtgtact
     4861 gcaataacgg catacattgg atctgtatga atttgaatgt tttcgccaaa cacatcgtgt
     4921 agctcatcaa tcaccaattg tgtaccttct tgattttctc cgtttaatac ataaaattca
     4981 taatcttgtg cattcgcatc ttttgaaatc actttaacag cttttttaat agcttttgac
     5041 attgttctta ctttatcaaa aacatctacc ttaccttctg tatttttaga cacttctagg
     5101 attggtttaa tttttaacat acctgcaagc ttagcagcca ttggagttaa tcttcctcct
     5161 ttagctaaat gatccaaatc ttcgggaatc aagaatcctc tcgaatgttc aacactttcc
     5221 tttaaacgtg agatgatttc ttctggatgt attttttgtt caactaattt ttgggcagat
     5281 aacactaaat atttttccac acctaaagtt gtatatatat ctaatgtatg gattttaact
     5341 ccatgccatt taccagatgc ctgtattgct tgatttgttc ctgataaacc attagacaat
     5401 gtaattaaaa taacatctgt aatgccatct tttttatatg attctaataa agcctcaata
     5461 tcgcctaatg gtggcatgct tgtttgaggc ataaagcctc tttctaagaa tgaatttaat
     5521 tcatctactg ttaaatccac cccatctaaa tacgttttat cttctatttg aacctgtaaa
     5581 ggtaaaaaat caattcctaa atcgtttgct tcttgttttg ttaatccaca accactttct
     5641 gttaatacac gaatcttcat taaaaccctc cttttataaa atcatcctaa tcctaacacg
     5701 aaatattaat tttctcaaat gtctgatata attggattga ggtgatttat atgagcgctt
     5761 tcatttcttt tgaaaatgtt aaaaagactt atcatatggg agaaatcgat atacatgcat
     5821 tagatgatgt aagttttgaa attgaacaag gtgagtttgt gatcattgct ggcgcaagtg
     5881 gtgctggtaa gagtactatc ctaaatttac ttggtggaat ggatactgtc agcgaaggaa
     5941 aaatcatcgt agatggtcgt gaaatcagtt cttataaaga aaaagatatg acactttatc
     6001 gaagatatga tgttggattt gtttttcaat tttataattt agtccaaaat ttaactcttc
     6061 gagaaaatgt cgagctagca acacaaattt gtaagaagcc acttgatatt gatgaaacta
     6121 tagatcttgt cggattgaaa gatagaactt acaattttcc tagtcagtta tctggtggtg
     6181 aacaacaacg tgttgcgatt gcgagagctt tggctaaaaa tccaaaactt ctactttgcg
     6241 atgagcctac aggtgcttta gattatcaaa ctggtaaaca agtccttaaa gttttgcaag
     6301 atacatgtaa gacacaaaat aaaacagtta tagtaattac ccacaactta gcattaacac
     6361 caatgggaga taaggtcatt aaagttcgta gtggaaaaat cgaaagtatt acatataatg
     6421 aacatccaat ggatgtcaat gatatagagt attaatatgc ctgctactta tttaaaggat
     6481 attttccgtg aaattaaaat ctcccttggc cgttttctct ctatcctttg tatcgtggcg
     6541 attggcgttg ctttttttgc aggaattaaa gccagtgctc cagatatgaa gaacagtgcc
     6601 gatacatatt ttgataaata taatgttcag gatattcaag tttattctac aattggtcta
     6661 acaaaaaaag atgtagctgc tatcaagaaa attaagggtg ttaagtctgt acaaccaagc
     6721 ttttcaatgg atacattatc tcaaattgat tccacgcaaa tggttataaa agtcatttct
     6781 tatggaattg atcaaaagat gaataagata cgtgtggtgg aagggcgaat gcctgaaaga
     6841 gaaaatgaat gtttggttga agctagttct gcaacgaata agctgtatgg aactttccac
     6901 attggagata ctatcaaact acaaagtggt acagatgaag ctttatctaa atcgttgaaa
     6961 catacaaaat ataaaattgt aggcacttgt tacaatccaa attacttatc ttatgaaaaa
     7021 ggttcaagta atattggttc tggaactgta aactcattta tatatattca aaatacaaat
     7081 gttttaaaag attattatac agaagtggat gtatgtgtaa aaggggctaa agatttagat
     7141 tgttatagtg atgaatattt tgatgtggtt gatccagtat taaagaagat caaaaagatt
     7201 tcaaacaaac aaatcgatgc acgtattcaa tcttatcagt ctgaattaga tgaaaaaaag
     7261 caagaagcca ccgataaatt taaggatgct gaaaatcaat tcaatgatgc tcaaaataag
     7321 attgattctg gactttctga aattcaaagc aatgaattaa agctacagaa ttctaaa
//
LOCUS       DBMDWL010000105         7330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3772_length_7330_cov_2.045096, whole genome shotgun sequence.
ACCESSION   DBMDWL010000105 DBMDWL010000000
VERSION     DBMDWL010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3772_length_7330_cov_2.045096"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            334..726
                     /locus_tag="ACZPDN_07770"
     CDS             334..726
                     /locus_tag="ACZPDN_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1298559.1"
                     /translation="MCIFCMIANGEIPSNKIYEDESVIAFLDINPTSYGHTLVVPKEH
                     CDSFLDCPDETRNHVFEVASKLANKLEQTLHCDGINILSNVHEAAGQSVNHFHIHLIP
                     RYKDNDRVTIEFHEIEKPDFEELMNKLK"
     gene            complement(744..1520)
                     /locus_tag="ACZPDN_07775"
     CDS             complement(744..1520)
                     /locus_tag="ACZPDN_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298560.1"
                     /translation="MDKQKIENKFIYFISLLGMVMILVLIAYFFFLRNVEVDIMDNAQ
                     YTYVGENGNASVVVSAKQGELNQRMQDFLNSVKYEVSPSSDLSNGDTIHVTATYDEAL
                     ANQYHYKPKSIEANVVVEGLANRYLALQDIPKTLIQDGRNAALDYVKENQDAIYKLDG
                     KEEKTPSLDKMKIVYSAYLKSNQKKNSDRFVYIVQMIYDSEVLYYMVCIPNINDSNEI
                     DTHNIYGEKAYLTQEELDGKDFNGYVDRVYSSKYQIEQKK"
     gene            complement(1531..3504)
                     /gene="recG"
                     /locus_tag="ACZPDN_07780"
     CDS             complement(1531..3504)
                     /gene="recG"
                     /locus_tag="ACZPDN_07780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1298561.1"
                     /translation="MQINELKITSRRLGLLVKMNILTVEDLLKYYPLRYDTVQTLPYA
                     SWNEKENVVFQGLICSAARVIRLSKNRSMTKFKVMSWNEELNITLFNRPWPSQFGFGK
                     TITIYGQYQGNNNVLASTYNFKPLDQQLGLHPVYPLIEGLKQSDIQAIMKEALKHVDI
                     LPQRVPQRYVEKYKLLDISTAYKWIHFPENEKQLHAAIRTLKYEEFLCFQCVMQSMHE
                     KKEIKTKKDFSVSTVKNWISNLPFELTKDQLSSIDDILYDLKSTNVMFRLVQGDVGCG
                     KTIVAQISMYANQLAGYQSALLAPTEILARQHVKNMHKLGLEATLYVSSLAAKEKKEI
                     LEKLENGKIFNVVGTHALFQENVVFHKLGLVIADEQQRFGVKQRRSLLEKGKCVDFLM
                     MSATPIPRTYAHFLYGDMDISSIHTMPKGRKSVVTKYFKTASMAPCLNDVLNFIKEGR
                     QCYVVCPAIEENDEYKMRNVFEIYEGMTKTLKDVRIGLLHGKMTSQEKEETMDKFSKH
                     EFDILVSTTVIEVGIDVANASVMVIYDAHRFGLSTLHQLRGRVARDKKQGYCFLLSSS
                     NDLQAIERLKKMEELKDGFEVSNYDLHMRGPGDILGIRQSGMPCLVFGDFEKDQAMME
                     MCIQDAKEIIQDQVDVDLLLYISNAIENAQYFD"
     gene            complement(3494..4249)
                     /locus_tag="ACZPDN_07785"
     CDS             complement(3494..4249)
                     /locus_tag="ACZPDN_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298562.1"
                     /translation="MKKLMSKLHFRSIYKTIVLTLVLCFLYNIAYLLMSASAMNVYIR
                     QSLEVVLSLLSSLGFYFICTKIWKAKNFSASAILKVFVLQGIYILIVSGILSNLVNVC
                     ANNTSLMLVLQLICAFCLVMMVPFQLIFYYGLIHDKNLKEFIPYALKKHQKKVLNWYC
                     TLLILIVVLDTMSGGLFSAAQGFNAQSILVGSMYMGNPMISWMMYLFLGVSFQSSLAS
                     MFTYLFVNFLMGFFYCVLELNYVSYVGSLLDAD"
     gene            4386..5444
                     /locus_tag="ACZPDN_07790"
     CDS             4386..5444
                     /locus_tag="ACZPDN_07790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1298563.1"
                     /translation="MNSKIKRILDFSTAFGPSGMEDEVSKLAMEDVKDICQVHDDTMR
                     NTYMHFKQDKEHKTTILLDAHADEVGFIVQAIKPNGTIRFLPLGGWDPKNIVSGEVWI
                     LNDKGEKISGIVASQPVHFLSEAKRNGKVDFDDLVIDVGATSATEVKEDFHISVGNFM
                     CPAVTCKYDEAHKIFLGKAFDCRIGCAAVMDVLHQLKDVNHNIEATLTTQEEVGERGM
                     DTAIKNLHPNIAICFEGCPSDDTFEQDYMIQSALKKGPMLRWFDRSYITSPRFMRYAI
                     DLAHKKDIPVQESVRKGGGTNAGITHRYNIPTIIIGIPVRFAHASFGICSEEDYDNAV
                     NLAVEIIKNIDAETIRGF"
     gene            5445..6299
                     /locus_tag="ACZPDN_07795"
     CDS             5445..6299
                     /locus_tag="ACZPDN_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1298564.1"
                     /translation="MEKIAVVTDTGSNLSFAQAKELGIYLLPLQITIDETTYQDTLEI
                     STQDIYNELANGKMPKTSMAAYQKIYDLFEELKKDYDTIFAVPLTNGLSTNASTMQSI
                     ARELEMNVHVIDMYATCALEKHVAIWIKKLVDEHKSSEEILKIIQPAIDESNSLILVK
                     NLQHLKRGGRLTPMAAALAGLLKIYPVLHINKSTEGRIDVLNKVRTEKRADAYAIDKI
                     MDDIDIQHTHIYIIHSNFLEGANHFKKCFIEKGVPEQNIHIDYISSVIAVHTGLGCIA
                     IQYIREEN"
     gene            6303..6788
                     /locus_tag="ACZPDN_07800"
     CDS             6303..6788
                     /locus_tag="ACZPDN_07800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1298565.1"
                     /translation="METKNILITMKNGDQIKAELYPEIAPETVENFLNLIEKKFYDGL
                     TFHRVIKGFMIQGGCPIGNGTGGPGYTIHGEFASNGFKNDLKHTRGVLSMARAMDPNS
                     AGSQFFIMHQDAPHLDGEYAAFGKVIEGMENVDKIAKTPTTFNDRPRNPQVIDTITLV
                     D"
     gene            6788..>7330
                     /locus_tag="ACZPDN_07805"
     CDS             6788..>7330
                     /locus_tag="ACZPDN_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1298566.1"
                     /translation="MTLQQLRYVIQVVESGSFNSAAKALFISQPSLSKAIHDLEEEMS
                     ICLFVRTNKGILLSEQGEKFLGYARQVVEQANLLEEQYKNAVPARTVFAISSQHYAFV
                     VNAFVALVKEYNQSEYEFSLREQRTYDIIDDVKNHRSQLGILYLSHFNREVIQSILKK
                     EHLTFTKLFEAKPHVFVSKTN"
BASE COUNT         2490 a         1348 c         1116 g         2376 t
ORIGIN      
        1 ttatatttca agaaaaaacc aaattttccc atttttaact cttgcaaact catacatttt
       61 catgaattga gacaaaaaat catcaaaagt ggggcctaaa cactatatcg ttgaaatatc
      121 acatattttc ccacactttt cacattttat ttgacacaac ttatggtata cttctttaca
      181 agcaaggagt ttgaagacct tgccggtatg aaatcagtct atggtattca tactattttc
      241 tatcccctta ttgaaaagcc agacaccccc ttagtctggc ttttcatttt atctaaaacg
      301 atgtattatt tttaataagg aggacttaaa cctatgtgta ttttttgtat gattgctaat
      361 ggcgaaattc caagcaataa aatttatgaa gatgaaagtg tcatcgcctt tctagatatc
      421 aatccaactt cttatggaca cacccttgtt gttccaaaag aacattgcga ttcatttcta
      481 gattgtccag atgaaaccag aaatcatgtt tttgaagttg caagtaaact tgccaataaa
      541 ttagaacaaa ctctacattg tgatggcatt aacatcttaa gtaatgttca tgaagcggct
      601 ggacaaagcg taaatcattt ccatattcat ttaattccac gctataaaga taatgatcgt
      661 gttacgattg aatttcatga aatcgaaaaa cctgattttg aagaattaat gaataaacta
      721 aaataaagaa gtcgtcgact tctttatttt ttttgttcaa tttgatattt tgatgaatag
      781 acacgatcta catacccatt aaaatcttta ccatccaatt cttcttgcgt taaatatgct
      841 ttttctccat atatattatg tgtatcaatt tcattggaat cattgatatt tggaatacac
      901 accatataat aaagaacttc tgaatcataa atcatttgta caatatacac aaaacgatca
      961 ctattctttt tctggttcga ttttaaatat gcagaatata caattttcat tttatcaaga
     1021 cttggtgtct tttcttcttt tccatctaac ttatagattg catcctgatt ttcctttacg
     1081 taatcaagtg cagcatttct accatcttga atcaatgtct taggaatatc ctgcaaagcc
     1141 aaatagcgat tggcaagtcc ttcaacgaca acatttgcct ctatactttt aggtttgtaa
     1201 tgatattgat ttgctaaagc ctcatcatag gttgccgtaa catgaattgt atctccattg
     1261 gataaatctg aacttggact cacttcatat ttgactgaat tcaaaaagtc ctgcattctt
     1321 tgattcaatt ccccctgctt tgcgcttacc acaacacttg catttccatt ttctccaaca
     1381 taggtatatt gcgcattatc cataatatcc acttcaacat tacgcaaaaa gaagaaataa
     1441 gcaatcaata caagaatcat taccatacca agtaagctga taaaatatat gaatttattc
     1501 tctatttttt gtttatccat cattagtctc ctaatcaaaa tattgtgcat tttcaatcgc
     1561 attcgaaata tacaacaaga gatccacatc cacttgatct tgtataattt cttttgcatc
     1621 ctgaatacac atttccatca tagcctggtc tttttcaaaa tcaccaaaca caagacaagg
     1681 cataccactt tgtcttattc ctaaaatatc acctggaccg cgcatatgca aatcataatt
     1741 tgatacttca aagccatcct tcaattcttc catctttttt aaacgctcaa tcgcctgcaa
     1801 atcatttgaa gatgacaaca agaaacaata tccttgtttt ttgtcacgag ctacacgtcc
     1861 tcttaactga tgtaatgtac ttaagccaaa gcgatgcgca tcataaatga ccatcacact
     1921 cgcattggct acatcaatcc caacttcgat aactgttgta gaaacaagaa tatcgaattc
     1981 atgcttagaa aacttgtcca tcgtctcttc cttttcttga cttgtcattt tcccatgcaa
     2041 caagccaatt cgcacatcct ttaatgtctt tgtcattccc tcataaattt caaacacatt
     2101 acgcatctta tactcatcat tttcttcaat cgcaggacat acgacataac attgtcttcc
     2161 ttctttaata aagtttaaaa catcatttaa acaaggtgcc atacttgccg ttttaaagta
     2221 ttttgtgaca acactctttc ttcctttagg catcgtatga atcgaactaa tatccatatc
     2281 tccatataag aaatgcgcat acgttctagg aatcggggtt gcggacatca ttaaaaaatc
     2341 aacacacttt cccttttcta acaaagacct tctttgtttc accccaaaac gctgttgttc
     2401 atccgcaatc acaagaccta atttatgaaa tacaacattt tcttgaaaca aagcatgtgt
     2461 tcccactaca ttaaaaattt tgccattttc aagtttctct aaaatttctt tcttttcctt
     2521 agccgcaaga cttgaaacat ataaagtcgc ttcaagacct aatttatgca tattctttac
     2581 atgttgtctt gccaaaattt cagttggtgc aagtaaagcc gattgataac ctgcaagttg
     2641 attggcatac atactaattt gtgccacaat cgtcttgcca cagccaacat ccccttgaac
     2701 aaggcgaaac atcacatttg tactttttaa atcatataga atatcatcaa tacttgaaag
     2761 ctgatctttt gtcaattcaa aaggaagatt tgaaatccaa ttttttactg tagaaactga
     2821 aaaatccttt tttgttttaa tttctttctt ttcatgcatc gactgcatta cacattgaaa
     2881 acacaaaaac tcttcatatt ttaatgtacg aatggctgca tgcagctgct tttcattttc
     2941 cggaaaatga atccacttat aagcggttga tatatctaat aacttatact tttcaacata
     3001 tctttggggt actctttgag gcagaatatc cacatgttta agcgcctctt tcataatcgc
     3061 ttgaatatcc gactgtttta agccttcaat taatggatat acaggatgta aacccaattg
     3121 ttgatctaaa ggtttgaaat tataagtgga agctaataca ttattatttc cttggtattg
     3181 cccataaatc gttatcgtct taccaaaacc aaattgactt ggccatggac gattaaataa
     3241 agtaatattt aattcttcat tccatgacat cactttaaat ttagtcatcg aacgattctt
     3301 agataaacgg atcactcgtg ctgccgaaca aatcaagcct tgaaaaacaa cattttcttt
     3361 ttcattccac gaagcataag gcaaagtttg taccgtgtca taacgcaaag gataatattt
     3421 caataaatct tcaaccgtta agatattcat ctttactaaa aggcccagtc ttcgactggt
     3481 tattttcaat tcattaatct gcatctaaaa gacttcccac ataagaaaca tagtttagtt
     3541 caagaacgca atagaaaaag cccatcaaaa aattcacaaa taaataagta aacatcgatg
     3601 caaggctgga ttgaaagctt acacctaaaa ataaatacat catccaagat atcatgggat
     3661 tacccatata catagagccc actaaaatac tttgggcatt aaaaccttgg gctgcactaa
     3721 acaaaccacc tgacattgta tccaatacaa cgattaagat gagtagtgta caataccagt
     3781 tcaacacctt tttctgatgt ttttttaatg cataaggaat aaattccttt aagtttttgt
     3841 catgaatcaa accataatag aaaatcaact ggaatggcac catcataact aagcagaatg
     3901 cacaaataag ttgtaacact aacattaagc ttgtattatt tgcgcaaaca tttacaagat
     3961 ttgaaagaat tccactcaca atcaagatat aaataccttg tagtacgaat acttttaaaa
     4021 ttgcagaagc cgaaaagttc tttgccttcc aaatctttgt acagatgaag taaaagccta
     4081 gacttgataa taagcttagt acaacttcta gactttgtct tatatagaca ttcatagcag
     4141 atgcactcat caacaaatat gcgatattgt ataaaaagca taaaactaac gttaatacga
     4201 ttgttttata tatacttcta aaatgtagtt ttgacattaa ttttttcatt gaaaaatctc
     4261 ctttttggtt aagatacatg tattctaatc ttttgtccaa atacaatttg attcaaatga
     4321 ttagattcac atccacaagc atgtatctgt gctataatat taacaaattt atggaggaaa
     4381 tgtatatgaa ttcaaaaata aaacgaattc ttgacttctc aactgccttt ggaccaagtg
     4441 gcatggaaga tgaagtatcg aaattagcca tggaagatgt caaagacatt tgtcaagttc
     4501 atgatgacac aatgcgtaat acatatatgc attttaaaca agataaagaa cacaagacaa
     4561 ccattctttt ggatgcacat gcagatgaag ttggttttat tgtgcaagcc atcaagccaa
     4621 atggtacaat tcgcttttta ccacttggtg gatgggatcc aaaaaacatc gtatcaggtg
     4681 aagtctggat tctaaatgat aagggtgaaa aaatttctgg tattgtcgca agtcaacctg
     4741 ttcatttctt atcggaagca aaacgtaatg gaaaagtaga ttttgatgat ttagttattg
     4801 atgtaggagc tacaagtgct actgaagtta aggaagattt ccatatttct gtaggtaatt
     4861 tcatgtgtcc tgccgttact tgtaaatatg atgaagccca caaaatcttt ttaggtaaag
     4921 cttttgattg ccgtattgga tgtgctgccg taatggatgt tcttcaccaa ttaaaagatg
     4981 taaatcataa cattgaagcc acattaacaa cgcaagaaga agtgggtgaa agaggtatgg
     5041 atacggccat taaaaactta catcctaata ttgcgatttg ttttgaaggg tgtccaagtg
     5101 atgatacttt tgaacaagat tacatgattc agtcggctct taaaaaaggt ccaatgttac
     5161 gttggtttga tcgttcatac atcactagcc ctcgttttat gcgctatgcg atcgacttag
     5221 cccacaaaaa agatatccct gtacaggaaa gtgtaagaaa aggtggagga accaatgcag
     5281 gtatcacaca tcgttacaac ataccaacga taatcattgg aattcctgtt cgttttgcgc
     5341 atgcttcttt tggcatttgt tctgaagaag attatgacaa tgctgtgaat cttgcggttg
     5401 aaatcattaa aaatatcgat gctgaaacga ttcgaggttt ctaaatggaa aaaatcgcag
     5461 ttgtaacaga tacgggttca aatttatctt ttgcgcaggc aaaagaatta ggaatctatc
     5521 tactcccttt acagattacg attgatgaaa caacctatca agatacatta gaaatttcta
     5581 cccaagatat atataacgaa ttagcgaatg gtaaaatgcc caaaacaagt atggctgctt
     5641 atcaaaaaat ttatgattta tttgaagagc taaagaaaga ctatgacaca atatttgccg
     5701 ttcctttgac aaatggatta tctacaaatg ctagcaccat gcaaagcatt gcacgtgaac
     5761 tcgaaatgaa tgtacatgtc attgatatgt atgcaacatg cgcacttgaa aaacatgttg
     5821 cgatttggat caagaagttg gtggatgaac ataaatcttc agaagagatt ttaaagatca
     5881 ttcaacctgc tattgatgaa tccaactcat tgattcttgt caaaaatctt caacatttaa
     5941 aacgcggtgg aagattaacg ccaatggctg cagcacttgc gggtttatta aagatctatc
     6001 ctgttttaca tataaataaa agtacagaag gtagaattga tgttttaaat aaagttcgta
     6061 cagaaaaaag agccgatgcc tatgcgatcg ataaaattat ggatgatatt gatattcaac
     6121 atacgcatat ttatattatt cattctaatt ttttagaggg ggccaaccat tttaaaaaat
     6181 gttttatcga aaaaggtgta cctgaacaaa atattcatat agactatatt agttctgtaa
     6241 ttgccgtaca tactggtctt ggttgtatcg caattcaata tataagagag gaaaactaaa
     6301 acatggaaac aaaaaatatt ttaatcacaa tgaaaaatgg agatcaaatc aaagctgaac
     6361 tatatcctga gattgctcca gaaacggttg aaaacttttt gaatctaatc gaaaagaaat
     6421 tctatgatgg acttactttc caccgtgtta tcaaaggatt tatgattcaa ggtggatgcc
     6481 ctatcggaaa tggtactggt ggtccaggtt atacaattca tggagaattc gcatctaatg
     6541 gatttaaaaa cgatttaaaa cacactcgtg gagttttatc tatggcacgt gctatggatc
     6601 caaatagtgc aggaagtcaa ttcttcatta tgcaccaaga tgcacctcat ttagatggtg
     6661 aatatgctgc gtttggaaaa gtgattgaag gtatggaaaa tgtggacaaa attgcaaaga
     6721 cccctactac attcaatgac cgccctagaa atcctcaagt gattgataca atcacacttg
     6781 ttgactaatg acgctacaac aattgcgcta cgtgatccag gtcgttgaat ctggatcttt
     6841 taattccgca gctaaagcct tatttatttc acaaccatct ttatcaaaag ccattcatga
     6901 tttagaagaa gaaatgtcca tctgtttatt tgttcgtacg aacaaaggta ttttactttc
     6961 tgaacaaggt gaaaaatttt taggatatgc tagacaagtc gttgaacaag caaacttact
     7021 tgaagaacaa tataaaaatg ccgttcctgc aagaactgta tttgcgatat caagtcaaca
     7081 ttatgccttt gtggtcaatg cctttgttgc cttagtcaaa gagtataatc aaagtgaata
     7141 tgaattttct ttaagagaac aaagaacata cgatattatt gatgatgtta aaaaccatcg
     7201 cagtcaattg ggaattcttt atctttctca ttttaatcgt gaagttattc aaagtatttt
     7261 aaagaaagaa catttaacat tcacaaaact atttgaggct aaacctcatg tctttgtatc
     7321 taagacaaat
//
LOCUS       DBMDWL010000106         7283 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3820_length_7283_cov_2.661236, whole genome shotgun sequence.
ACCESSION   DBMDWL010000106 DBMDWL010000000
VERSION     DBMDWL010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3820_length_7283_cov_2.661236"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            199..408
                     /gene="rpmE"
                     /locus_tag="ACZPDN_07810"
     CDS             199..408
                     /gene="rpmE"
                     /locus_tag="ACZPDN_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR1298567.1"
                     /translation="MKQGIHPNYHKVKCICTSCGAEFETGSTIKGDELRVDTCSNCHP
                     FFTGRQRFAAAQGRIEKFNKKYGLK"
     gene            635..1801
                     /locus_tag="ACZPDN_07815"
     CDS             635..1801
                     /locus_tag="ACZPDN_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1298568.1"
                     /translation="MKRAKRMDLFQSSIFTELKNMKLDYMKRTGNTTIDFSVGSPNIA
                     PEPSIMKALQDAIMVLENYKYAINELPEMKAAIHDWYKDRYHVELTNDEMICLQGSQE
                     ALSTLFLAMCDPGDIVLVPDPYYPIFSDGPKIAGAKVEYMPLLEEYNYIIQLDKIDEE
                     IAKKAKMMIVSYPNNPTCAIAPDSFYEELIAFAKKYDIVVLHDNAYSELVFDGKVGKS
                     FLSFAGAKEVGIELNSFSKTYGMAGARLGVCVGNKEVIECYNTLKSNMDYGIFLPVQY
                     AGIEALRHGGASIHSTCQAYQDRRDALCEIFDSVGWHIASSPATMFAWAKIPDQYEDS
                     YVFTKDLLEKTGIVVTPGQAFGVHGKKYVRLALVQDIEDIKEAAQRIKKSEIFK"
     gene            1839..3005
                     /locus_tag="ACZPDN_07820"
     CDS             1839..3005
                     /locus_tag="ACZPDN_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR1298569.1"
                     /translation="MKCNRCQNEDPKLFAYDHGIYYCRKCIGFSRLEVHEKIKPVELM
                     HKTWKGKPKLDYELTASQKLASHDVYKHLSNGKDVILYASTGAGKTEICFESICGYLA
                     QGKKVGFAISRRQVVLEIATRLRKAFPELSICEVAQGYTQITDADIIVCTTHQLYRYP
                     YAFDLLILDELDAFPFAGNEVLQAIANQACVGQKLMLSATPDEESMRLVEEKKTELVC
                     LFERPHKHPLIVPKVIQVSVFLQVLIVIYECIKFCRDNKQTLVFVPRKRDGTWLKMIL
                     NLFVKTSLVHSSTENKDEILDCFRNKELDVLVCTTLLERGITIPSVQVVVFKGNHSVF
                     TTASLIQIFGRVGRSFKDPKGEGICLCQFSNQSIKDCVYQISKMNDTVYAATKK"
     gene            3002..3616
                     /locus_tag="ACZPDN_07825"
     CDS             3002..3616
                     /locus_tag="ACZPDN_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012383.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR1298570.1"
                     /translation="MSEIRNFDIFFYNNLIICPDCMKNFKVHKQTYMIKGILIHVLYE
                     YDEFLERLFFQYKEQRDIVLKEVFLDSHKYLSKTFKHYCVCSLCSSDAKRMERGFEPV
                     VDIYATLGVFVHSPFYKKENVKQSSKSRLERAHIKDVLALKEMYPLPKKKKILLVDDV
                     CTTGNTLYAALDLIHPDCIFVIAAHPLWIKANKENIVVKSNGFW"
     gene            3644..4975
                     /locus_tag="ACZPDN_07830"
     CDS             3644..4975
                     /locus_tag="ACZPDN_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR1298571.1"
                     /translation="MDKKRKNENDEISQLLDSFQADQEDTLEQKMDRFTKTQNRSRVK
                     KHKPEPEIPFQSKKEEPSLGDTFVVGSKDLNEPKEDLSATRTVMWNADELKQETDATN
                     KTVVIDDDEIQSLLEEGKGPQLKREVINKKKDSKKKASKKNNSKTGLYIGLGVVGVLL
                     ISLLIFGGIKLVTSGLSGSDEKTEEVQKKNYQELLDFANNYDSLSSSEKKEIVDLESK
                     YNSLTRKQREKIDEILEKATGKNFNELLAKANKKTNKNKKNNNTEIAEKKAELRKKIS
                     DLKNQLSEANADLERAQKAQESAQSDVNSLEQQVNEAQSTLDKAQSAEDSAQTAYDNL
                     VSILKRRAELDNKPNDQITQAELSELSDIRTNHPNLENEVNLAKDDLDAAKSANTEAK
                     NKSNKSSLQSKLSTAQTKLNSASSDVKDAQSKVDSLNSQINDYQSQLDDLD"
     gene            5066..6067
                     /locus_tag="ACZPDN_07835"
     CDS             5066..6067
                     /locus_tag="ACZPDN_07835"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR1298572.1"
                     /translation="MRNAIVLAAGKGTRMKSDRSKVMHTIIDRSMLAYIIDALRAVSV
                     QRIVVIVGYQAESIKEAFPDVEFALQQPQLGTGHAVMQATQLKDEDGDTLIINGDGPC
                     IQPETLEKLFQTNQDASLTLLTSVLDDGAHYGRIVRDENGHVQAIVEAKDCTEEQKKI
                     CEINAGMYCFKNRDLFDNLDKLTTNNAQNEYYLTDLVKILANQGKVVKGLVVEDRDEV
                     MGINDCVELNKAYEWMRNRINNNWLKEGVQIVDPKRTVIGKDVKIGHDVIIHPNVEIL
                     GKTEIGDFVEILPGSYLNNSKIENCAVVDSSKIVDSCISEGTIVGPMSYVSNNKIIK"
     gene            6096..7052
                     /locus_tag="ACZPDN_07840"
     CDS             6096..7052
                     /locus_tag="ACZPDN_07840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR1298573.1"
                     /translation="MKNTIVFALSSSVGLAKDICKELGLELGKCEVKHFADGEILVEL
                     EESVRGKHVYFVQSTNKPVNSNLMEILIGIDACKRASAASITAIIPYFGYARQDRKAK
                     PRQPITSKLVASLLERAGADRVVTVDLHATQIQGFFDIPADDITANGLIADYFKHKEG
                     LGEIVVVSPDHGGAVRARNLANALGAPIAIIDKRRPKPNVAEAMNLIGDVDGKTAIII
                     DDMVDTAGTLTAGIKMLKDKGAKAVYASCSHGILSGPAIDRLKAAELAGFVCTNTIDQ
                     TENQKLYPEMTVLSMAPLLAGLIHAVEENSSLSEVLAHAFDD"
     gene            7054..>7283
                     /locus_tag="ACZPDN_07845"
     CDS             7054..>7283
                     /locus_tag="ACZPDN_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HIR1298574.1"
                     /translation="METLFIGYPPCSTCKKAYKALLDLGIEATYRNIKEENPTKEEIQ
                     SWIDRGVKLKKLFNTSGMLYRELNIKEKRETYT"
BASE COUNT         2655 a          923 c         1477 g         2228 t
ORIGIN      
        1 ataataatag tgtacaccaa tacactatta aagtatataa aaaaataaat aatcttcaaa
       61 catgaaaaat tgaagattat ttcatgcgta atcactaatt ttaaaaaaac aacttgcgat
      121 tttgaaaatg gcttgctata attgtatagc acttactttg ggtcgatatg acttaaggcg
      181 gaaggaggat acatcgatat gaaacaagga attcatccta attatcataa agttaaatgt
      241 atctgtacat cttgtggtgc agaattcgaa acaggtagta caatcaaagg tgatgaatta
      301 cgtgtagata cttgcagtaa ctgccaccca ttcttcacag gtcgtcaacg tttcgctgct
      361 gctcagggac gtattgagaa atttaacaag aaatatggtt tgaagtagta atataatgac
      421 agtgtcagaa atggcactgt tttttatttt ggttataaaa aaattacaat ttaattttta
      481 aaatccttta aaataggctt attttgatag tttggttata ttaaaatcaa caaatgtaca
      541 caaagtagaa tagaaatcat tgtggtgttt gaaaactttg ttttgtacgc ttataataaa
      601 gtcaataaat tagaaaatag cgaggtattt tgaaatgaaa agagcaaaaa gaatggattt
      661 atttcaatct tctatattta cagaattaaa gaatatgaaa ttagattaca tgaaacgtac
      721 aggaaatacg acaattgatt tttctgtagg ttcacctaat attgcaccag aaccatcaat
      781 tatgaaggct ttacaagatg caatcatggt acttgaaaat tataaatatg cgatcaatga
      841 attacctgaa atgaaggctg ccattcatga ttggtataaa gatcgttatc atgtagaact
      901 tacaaatgat gaaatgattt gtttacaagg ttctcaagaa gctttaagta cattattttt
      961 agctatgtgt gatccaggtg atattgtatt ggttccagat ccatattatc caatctttag
     1021 tgatggacct aaaattgcag gcgctaaagt agaatatatg cctttgttag aagaatacaa
     1081 ttatattatt caattggata aaattgatga agaaatcgca aaaaaggcaa agatgatgat
     1141 tgtatcctat ccaaacaatc caacttgcgc aattgctcca gattctttct atgaagaatt
     1201 aattgcgttt gcgaagaagt acgatattgt tgtattacat gataatgcat atagtgaatt
     1261 ggtatttgat ggaaaggtag gaaagagttt cttatctttt gcaggtgcta aagaagttgg
     1321 aattgagtta aattcattct ctaaaacata tggaatggca ggtgcaagac ttggtgtatg
     1381 tgtaggaaat aaagaagtaa tcgaatgtta taacacactt aaatcaaata tggattatgg
     1441 cattttctta cccgtacaat atgcaggtat agaagcttta agacatggtg gtgccagcat
     1501 tcattcaact tgtcaggctt atcaagatcg aagagatgca ttgtgtgaaa tatttgattc
     1561 tgttggatgg catattgctt caagtcctgc aacaatgttt gcgtgggcaa agattccaga
     1621 tcaatatgaa gattcttatg tcttcacaaa agatttgttg gaaaaaacag gaatagtcgt
     1681 aacgcctggt caagcctttg gagtccacgg gaaaaaatac gttcgtttag ctttagtgca
     1741 agatatagaa gatattaaag aagcggctca aagaataaaa aaatcagaaa tttttaaata
     1801 aagtgcaaat tttgcacttt tttttcgtat aagagtatat gaagtgtaat cgatgtcaaa
     1861 atgaagatcc aaaattattt gcgtatgatc atggaattta ttattgtcgt aaatgcattg
     1921 gtttttctag attggaggtt catgaaaaaa taaaaccagt agaattaatg cataaaacgt
     1981 ggaaagggaa acctaaatta gattatgaac tgactgcttc gcaaaaatta gctagtcatg
     2041 atgtatacaa gcatttaagt aacggaaaag atgtgattct ttatgcaagt acgggagcag
     2101 ggaaaacaga gatttgtttt gaaagtatat gtggatattt agcacagggg aaaaaggtag
     2161 gatttgcgat tagtcgtaga caagtggtgc ttgaaatagc tacaagactt agaaaggcat
     2221 ttccagaatt gagtatatgt gaggtagccc aaggatatac acaaataaca gatgcagaca
     2281 ttattgtgtg tacgactcat caactttatc gatatccata tgcgtttgat ttattaattt
     2341 tagatgaatt agatgcattt ccttttgcgg gtaatgaagt gttgcaagcc atcgcaaatc
     2401 aagcgtgtgt tggtcagaag ctaatgttga gtgcaacacc ggatgaagaa agtatgaggc
     2461 ttgtagaaga gaaaaaaaca gaacttgtat gcttatttga aaggcctcat aagcatcctt
     2521 tgattgtgcc taaagtgatt caggtttctg tgtttttaca agtccttatt gtgatttatg
     2581 aatgcataaa attttgtagg gataataaac aaacccttgt gtttgtgcct cgtaaaagag
     2641 atggaacatg gttaaaaatg attttgaatc tctttgtgaa aacatcattg gttcattctt
     2701 cgactgaaaa taaggatgaa atattagatt gttttcgtaa taaagaatta gatgttttag
     2761 tttgtacaac acttttagaa agaggaatca caataccaag tgttcaggtt gttgttttta
     2821 aagggaatca ttctgtattt acaacggcta gtttaattca gatttttggc cgtgtgggaa
     2881 gaagttttaa agatcctaaa ggagaaggta tatgtctatg tcagttttca aatcaatcta
     2941 tcaaagattg tgtttatcaa atatcgaaga tgaacgatac tgtttatgct gccacaaaaa
     3001 aatgagtgaa atacgaaatt ttgatatatt cttttataat aatttgatta tttgtccgga
     3061 ttgtatgaaa aattttaaag tgcataaaca gacatatatg ataaagggaa tcttaataca
     3121 tgtattgtat gaatatgatg agtttttaga aagattgttt tttcaatata aagaacagag
     3181 ggatattgtt ttaaaagagg tttttttaga ttctcataaa tatttgagta agacttttaa
     3241 acattattgt gtgtgtagtt tatgtagtag tgatgctaag cgcatggaaa gaggatttga
     3301 gccagtggtg gatatatatg cgactttagg tgtctttgtg cattcgccgt tttataaaaa
     3361 agaaaatgta aaacaatctt caaaatccag gcttgaaaga gcacatataa aagatgtatt
     3421 agctttaaaa gagatgtatc cactgcctaa aaaaaagaaa atattgcttg tggatgatgt
     3481 gtgtacaacg ggaaatacat tgtatgctgc tttggatttg attcatccag attgtatatt
     3541 tgtgatagcg gcgcatcctt tatggattaa ggcaaataaa gaaaacatag tggtcaaatc
     3601 aaatgggttt tggtaaaata gtttcaaagg ggcgatagaa gctatggata agaaaagaaa
     3661 gaacgagaat gatgagattt ctcaattgct agattcgttt caagcggatc aggaagatac
     3721 attagaacaa aaaatggatc gatttacaaa gactcaaaat cgtagtcgag ttaagaaaca
     3781 taaacctgaa ccggagattc catttcagtc aaaaaaagag gagccatcat taggagatac
     3841 gtttgtagtt ggatcaaagg atttgaatga gcctaaagaa gatttgtctg caacacgcac
     3901 agtgatgtgg aatgcggacg aactaaaaca agagacggat gctacaaaca aaacagttgt
     3961 gatcgatgac gatgagattc aatctttatt agaagaaggt aaggggcctc aactaaaaag
     4021 agaggtcatc aataagaaga aggattctaa gaaaaaagca tctaagaaaa acaactctaa
     4081 gacaggatta tatattggat taggtgttgt aggtgtttta ttgatttcat tattgatttt
     4141 tggtggtatc aaactagtaa ctagtggatt atcgggttcg gatgaaaaaa cagaagaagt
     4201 tcaaaagaag aattatcaag aattattaga ttttgcgaat aactacgatt cattatcttc
     4261 tagcgagaag aaagaaattg ttgatcttga gagtaaatat aacagtttga ctcgtaaaca
     4321 aagagaaaaa attgatgaaa ttttagagaa ggctacgggt aaaaacttta atgaattgct
     4381 tgcgaaagca aacaaaaaaa cgaataagaa taaaaagaac aacaacacag aaattgcgga
     4441 aaagaaagca gagttaagga aaaaaatcag tgatttaaag aatcagcttt cagaggctaa
     4501 cgcggattta gagcgtgcac aaaaagcaca ggaatctgca cagagtgatg tgaactcttt
     4561 ggagcaacaa gttaatgaag cacaaagtac actagataag gcgcaaagtg cagaagattc
     4621 ggctcaaact gcttatgata atttggttag tatattaaag cgaagagcgg aacttgataa
     4681 taaaccaaat gatcaaatta cacaggctga actttctgaa ttatcggata tcagaacaaa
     4741 tcatccgaat ttagaaaatg aagtgaattt agctaaagat gatttggatg cagctaaatc
     4801 tgcaaataca gaggcaaaaa ataaatcgaa taaatcttct ttacaaagta agttgagtac
     4861 agcacaaaca aaattaaata gtgcaagttc agatgtaaaa gatgcacaat caaaagtgga
     4921 ttcattaaat tcacaaatca atgattatca aagtcaatta gacgatttag actagacaat
     4981 acatttttgt attgtctttt tctattgtaa gcttttaatg tcttacgatt tttgttagaa
     5041 tagatacgga aagtcaggtg aaaatatgcg taatgcgatt gttctggcgg ctggaaaagg
     5101 aactcgaatg aaatcagatc gtagtaaagt aatgcatacg attattgatc gttcaatgtt
     5161 ggcttatatc attgatgctt taagagctgt atctgtacaa agaatcgttg tgattgttgg
     5221 atatcaagca gagagtatca aagaagcttt tccagatgtt gaatttgctt tgcagcaacc
     5281 ccaattaggg actggtcatg cggttatgca ggctacacaa ttaaaggatg aagatggaga
     5341 cacattaatt atcaatggtg atggtccttg tattcaacct gaaactttgg aaaaactatt
     5401 ccaaacaaat caagatgcat cattaacttt attaacttct gtattagatg atggagctca
     5461 ttatggtcgt attgttcgtg atgaaaatgg acatgttcaa gcgattgtag aggcaaaaga
     5521 ttgtacagaa gaacaaaaga aaatctgtga aatcaatgcg ggtatgtatt gtttcaaaaa
     5581 tagagattta tttgataatc ttgataagct aacaacaaat aatgctcaaa atgaatatta
     5641 tttaacagat ttagttaaaa ttttagctaa ccaaggtaaa gttgttaagg gattagttgt
     5701 tgaagaccgt gatgaagtca tgggaattaa cgattgtgta gaattaaata aagcgtatga
     5761 atggatgcga aatcgaatca acaataactg gctaaaagaa ggggtacaaa ttgtagatcc
     5821 taagcgcaca gttattggta aagatgtaaa aattggacat gatgttatta ttcatccaaa
     5881 tgtcgaaatt ttaggcaaaa cagagattgg agatttcgtt gagattttgc caggttctta
     5941 tttgaataat tcaaaaattg aaaattgtgc tgtagtagat tcaagtaaaa tcgtcgattc
     6001 ttgcataagt gaaggaacta ttgtgggacc tatgtcttat gtaagtaaca ataaaattat
     6061 aaaatagcct gatagggtat gagaaaggat aaatcatgaa aaacactatt gtatttgctt
     6121 tatcatctag cgttggttta gcaaaagata tttgtaaaga attaggattg gaattaggaa
     6181 aatgtgaagt aaaacatttt gctgacggag aaattttggt agaattagaa gaatctgttc
     6241 gtggaaaaca tgtatatttt gtacaaagta caaataaacc tgtaaactca aatttaatgg
     6301 aaattctaat tggtattgat gcttgtaaac gtgcaagtgc tgctagtatt acagctatta
     6361 ttccatattt tggatatgca cgtcaggatc gtaaggcaaa acctcgtcaa ccaattactt
     6421 ctaaattagt agctagttta ttggaaagag ctggagctga tcgtgttgta acagttgatt
     6481 tgcatgctac tcagattcaa ggattctttg atattccagc agatgatatt actgcgaatg
     6541 gattgattgc tgattacttc aaacataagg aaggattagg agaaatcgtt gtagtatctc
     6601 ctgatcacgg tggagctgta cgtgctcgta atttagcaaa tgcattaggt gcacctatcg
     6661 caatcatcga caaacgtcgt ccaaaaccaa acgtagcaga agctatgaac ttaattggag
     6721 atgttgatgg aaaaactgca attatcattg atgatatggt agatacagct ggaacgttaa
     6781 ctgctggtat taaaatgtta aaagacaaag gtgcaaaagc tgtatacgct tcatgttcac
     6841 atggtattct ttcaggacct gctattgatc gtttaaaagc tgctgaatta gctggatttg
     6901 tttgtacaaa tactattgat caaacagaaa accaaaagct ttatccagaa atgactgttc
     6961 tttcaatggc accattatta gctggattaa ttcacgctgt tgaagaaaac tcttcactat
     7021 cagaagtatt agcacacgct tttgatgact aatatggaaa cgttgtttat cggttatcca
     7081 ccttgttcaa cttgtaaaaa agcatataag gctttgttag atttagggat tgaagctaca
     7141 tatagaaata ttaaagagga aaatccgaca aaagaagaga ttcaaagttg gattgatcga
     7201 ggtgtgaaat taaaaaaatt gtttaacaca agtggtatgt tgtatcgtga attaaatata
     7261 aaagaaaaac gtgagacata tac
//
LOCUS       DBMDWL010000107         7256 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3838_length_7256_cov_3.115723, whole genome shotgun sequence.
ACCESSION   DBMDWL010000107 DBMDWL010000000
VERSION     DBMDWL010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3838_length_7256_cov_3.115723"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(112..465)
                     /locus_tag="ACZPDN_07850"
     CDS             complement(112..465)
                     /locus_tag="ACZPDN_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1298575.1"
                     /translation="MIGTHCDCKPKNTELVQKVKENMPAFDEIMNLSDFFKVMGDSTR
                     LQLLLSLQQSEMCVSDLANVLDMTKSAVSHQLKALRLSKLIKSRKEGKTVYYSLDDEH
                     IETILGMAFDHTKEH"
     gene            605..823
                     /locus_tag="ACZPDN_07855"
     CDS             605..823
                     /locus_tag="ACZPDN_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR1298576.1"
                     /translation="MKKSYKIDVDCANCANKMEVAANDTEGVKSAVVNFMMLKMNVEF
                     EDGVQPKEVMARVLENCKKVEDDCEIFF"
     gene            914..2782
                     /locus_tag="ACZPDN_07860"
     CDS             914..2782
                     /locus_tag="ACZPDN_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1298577.1"
                     /translation="MNKKQKKMLKRIIVASILFIIIKVVKLPEYIEIPLFLVAYLEIG
                     YDILLKAFKGIRNRQVFDENFLMAVATVGAIGLKSFDEATAVMLFYQIGEWFQSYAVG
                     KSRKNITELMDIRPDYANVEDEDGNLEQVDPDEVEIGTIIVVKPGEKVAIDGIVIEGS
                     STLNTAALTGESLPREVSENDEVISGCINMTGLLKIKTTKEFGESTVSKILDLVENAS
                     SKKSRSEAFISRFARIYTPAVCYSALALFLLPPVFNLIMSNPADFGTWLYRALTFLVI
                     SCPCALVISIPLSFFAGIGGASNQGVLVKGSNYLEALASCKYVVFDKTGTMTQGVFEV
                     AGIHHANIPEEKLLEYAAMAESYSTHPISKSLLKAYGNTIDKSRIENIEEISGHGVKA
                     VIDGVQVLAGNDKLMKMYNIPYQECHSVGTIVHMAIDGKYAGHIVISDMLKPHAKEAI
                     EALKKAGIKQTVMLTGDVKSVADKVASELHIDKVYSELLPQDKVNKVEELLSLKHSKE
                     NLAFVGDGINDAPVLSRADIGIAMGALGSDAAIEAADIVLMDDDPLKISKAIKIAKKC
                     IHIVYENIYFAIGVKLICLLLGAIGIANMWVAIFADVGVMIIAVLNAIRALNVKNL"
     gene            2870..3739
                     /locus_tag="ACZPDN_07865"
     CDS             2870..3739
                     /locus_tag="ACZPDN_07865"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1298578.1"
                     /translation="MQELKPIKEGKVREIYDNGDNLIMVATDRISCFDVILNNEVTKK
                     GTVLTQMSKFWFDYTSDILPNHMISVDTKDMPEYFQQEKFEGKSMLCRKLNMLPIECI
                     VRGYITGSGWASYKETGKVCGIELPEGLQESQQLPEPIYTPSTKAEIGDHDENISFEQ
                     SIAHLEKHFPGHGEEYATKIKDCTIALYKKCAEYALSKGIIIADTKFEFGLDENGNIV
                     IGDEMLTPDSSRFWPADGYEAGHSQPSFDKQFARDWLKANPDNDWTLPQEIVDKTIEK
                     YLQAYTMLTGKTL"
     gene            3929..4561
                     /gene="tmk"
                     /locus_tag="ACZPDN_07870"
     CDS             3929..4561
                     /gene="tmk"
                     /locus_tag="ACZPDN_07870"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR1298579.1"
                     /translation="MKQGVFITFEGNDGSGKTTVCKAVQEELIKMGYDCIYTREPGGS
                     KIAESIRNILLDVNNTEMDNRTEALLYAASRRQHLKEVVLPALENKKIVLCDRFIDSS
                     LAYQGIARGIGLKEVWDINQFAIDGCMPEKTLFLSVSVETGKKRMNLRGDLNRLDQEE
                     SSFHKAVRQGYETLVSMYPERIEVIDAEPCKEEVLKSAMECVLKVIKEHE"
     gene            4554..5468
                     /locus_tag="ACZPDN_07875"
     CDS             4554..5468
                     /locus_tag="ACZPDN_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR1298580.1"
                     /translation="MNKEYLKKAQPVVYQTLSNALKNQRVANAYLFHGPKGSSKLETA
                     ILFAQSIVCEHTDNDGFACQECESCKRIENEESLDYMIVSNDRIKKKDIVDLQTFFES
                     TSAGKQNARIYILDHYDKATPDASNSLLKFLEEPSEGIYGILIADEKSNVLPTIQSRC
                     QAIHFKPESCEHLLLENTSEENAKMLAESGYTYDKAIELLSLPDFEELKQIALEYIEH
                     ASSFSQIEKMQTNIFVNKSDRMNKDWVQLWIQWVLFYVKNDRVNLPLEKKVKLYSILV
                     ENMDMLYRPVDLGLFMDKLYYDIRKAVI"
     gene            5468..6301
                     /gene="ricT"
                     /locus_tag="ACZPDN_07880"
     CDS             5468..6301
                     /gene="ricT"
                     /locus_tag="ACZPDN_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HIR1298581.1"
                     /translation="MNTEGKVTYKYIVYIQFEESKKAYTFGSDVKYYTNDVVVVETVR
                     GQELGKVCVPTVDFDALKVKGDIKPVLRKATQEDIKCKEENVEKAKEAMKICHECVAN
                     LKLDMHLISSEYTLDRTKVIFTYVSDDRVDFRQLLKDLAQHLHCRIELRQVGPRNKAK
                     IVGGIGNCGMECCCSRFMSDFDTVSINMAKNQMLALNIQKLSGQCGKLMCCLRFENEE
                     YTRMRKDLPKMNSIVSYQGKKYRISSMNVLQKQAKLENKEEVLFVDFKELWPDKDFNN
                     D"
     gene            6294..7139
                     /gene="rsmI"
                     /locus_tag="ACZPDN_07885"
     CDS             6294..7139
                     /gene="rsmI"
                     /locus_tag="ACZPDN_07885"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1298582.1"
                     /translation="MIRQKSFESNTSTLYLVPTPIGNLQEMTPRALDILKEVSVIAAE
                     DTRNTKKLLMAYGIDTMLMSHHEHNQQVSIPKIIERLENGEDVAVVSDAGYPLVSDPG
                     QKLVQETISHGFNVVSVSGANAAMNALVASGLSCYHYLFYGFLDNKSSKRKKQLEEIK
                     TIPYTMIFYEAPHRIEDTLQDMLEVLGNRQMCLARELTKVHEEYIRGTIEEVLEVATT
                     CKGEMVIVMDGFKQDEVVFDMYTAITKVDEYIESGMRTKEAIGLVAKEMACKKNELYE
                     AYHKR"
BASE COUNT         2499 a          923 c         1538 g         2296 t
ORIGIN      
        1 ttggctcttg acatatttat tttaaaacaa cgaaaaaaga tacccacaag tgagtatcac
       61 aactgtcacg ttgttgtcct ccttatgggt accattttat cgatcttcct cttagtgttc
      121 ttttgtatga tcaaaagcca tgcctagaat ggtttcaata tgttcatcgt ctaatgaata
      181 gtagacagtc tttccttctt ttctagattt gatgagttta gaaaggcgta aagcttttag
      241 ttggtgtgat acagcagatt tcgtcatatc taaaacgtta gctaaatcgg atacgcacat
      301 ttcactttgt tgaagggata ataaaagttg aaggcgtgta ctatctccca tcactttaaa
      361 gaaatcggat aggttcatga tttcatcaaa ggccggcatg ttttctttta ctttttgtac
      421 taattctgta tttttaggct tgcagtcaca atgtgtacca atcataagtt ctcctttttt
      481 ctttattata gttgaatgat tgaacaattg tcaaaaagtt gttgacaaca tgtatgattc
      541 ctatataatg aaagtgtaaa aagttgaata tgtgttcaac tattcaaacg tggagggaaa
      601 tcatatgaaa aagagttata agattgatgt agattgtgca aactgtgcaa acaaaatgga
      661 agtagctgct aatgatacag aaggtgttaa gagtgcagtt gtaaacttca tgatgttaaa
      721 gatgaatgtt gaatttgaag atggagttca acctaaagaa gttatggctc gtgttttaga
      781 aaattgtaaa aaagttgaag atgattgcga aattttcttt taatgaaaag taagatgcga
      841 aagcatctct atttttcggt tattatagtt gaatgattga acaattaatg tttttggaga
      901 ggtagagata agtatgaata agaaacagaa aaagatgtta aagcgaatca ttgttgcgag
      961 tattcttttt atcataatta aggtcgtaaa actacctgaa tacattgaga ttcctttgtt
     1021 tctagtggcc tatctagaaa ttggttatga tattcttctt aaggctttta aaggaattag
     1081 aaatcgtcag gtatttgatg aaaatttttt gatggctgtc gcaactgtag gcgctattgg
     1141 tttgaaatct tttgatgaag caacagctgt tatgttgttc tatcagattg gggaatggtt
     1201 ccagagttat gcggttggaa aaagtcgtaa aaatatcacg gaattaatgg atattcgtcc
     1261 ggattatgcc aatgttgaag atgaggatgg aaatcttgaa caggtagatc cagatgaagt
     1321 tgaaatcggt acaattattg tggttaagcc tggtgaaaaa gtggctattg atggaattgt
     1381 cattgaagga agttctactc taaatacagc ggctttaaca ggggaaagct tgcctagaga
     1441 agtcagtgaa aatgatgaag taatcagtgg ttgcatcaat atgacgggct tattaaaaat
     1501 taagacaaca aaagaatttg gggaatccac tgtatcaaaa attttggatt tggttgaaaa
     1561 tgcatcaagt aagaaatctc gttctgaagc ctttatttca cgttttgcga gaatttatac
     1621 gccagccgta tgttatagtg ctttggcatt gttcttactt ccacctgtat tcaatttgat
     1681 tatgtcaaat cctgctgact ttggtacatg gctatatcgt gccttaacat tcttagttat
     1741 ttcttgtcca tgtgcattgg taatcagtat tcctttgagt ttctttgccg gaattggtgg
     1801 cgccagcaat cagggcgttt tggttaaagg atcgaattat ttagaagcat tggcttcatg
     1861 taaatatgtt gtatttgata aaacgggtac aatgacgcaa ggtgtatttg aggtggcagg
     1921 cattcatcat gcgaatattc cagaagaaaa gttattagag tatgcggcaa tggctgaaag
     1981 ttattctacg catcctattt ctaaaagttt attaaaggcg tatggaaata caattgataa
     2041 atcacgtatt gaaaatatag aagaaataag tggtcatgga gttaaagctg taattgatgg
     2101 agtgcaagta cttgcaggta atgataaatt aatgaagatg tataatattc cttatcaaga
     2161 atgtcatagt gttggtacga ttgttcatat ggctattgac ggtaaatatg caggtcacat
     2221 tgtgatttcg gatatgttga agccgcatgc caaggaagca attgaagcat tgaagaaggc
     2281 tggcattaaa caaacagtca tgttgactgg ggatgtaaaa agtgtggcag ataaagtcgc
     2341 ttctgaatta catattgata aagtgtattc ggaattattg cctcaagata aggtgaataa
     2401 agttgaagaa ttattatctt taaagcactc aaaagaaaat cttgcctttg tcggagatgg
     2461 aatcaatgat gctccagtat tatcaagggc cgatataggt attgcgatgg gtgctttagg
     2521 cagtgatgca gccattgagg cagcggatat tgtattaatg gatgatgatc cattaaagat
     2581 ttctaaagcc attaagattg cgaaaaaatg tattcatatt gtatatgaga atatttattt
     2641 tgcgattggt gttaaattga tctgtttatt attaggtgcc attggtattg cgaatatgtg
     2701 ggttgcgatt tttgcggatg ttggagttat gattattgcc gtattaaatg cgattcgggc
     2761 attaaatgtt aaaaatttgt aaggaaatct taggatttcc ttattttcat attgtgttaa
     2821 atttgtttta gttgtatctt tgtcttgtac gcagaaaagg agattcgaga tgcaggaatt
     2881 aaaaccaatt aaagaaggaa aagtacgtga aatttatgac aatggggaca accttattat
     2941 ggttgcgaca gatcgtatca gttgttttga tgtaattcta aacaatgagg ttacaaaaaa
     3001 aggaactgtt ttaacacaga tgtcaaaatt ttggtttgac tacacaagtg atatcttacc
     3061 taatcacatg attagtgtgg atacaaaaga tatgccagaa tatttccaac aagaaaaatt
     3121 tgaagggaaa tcaatgttat gtcgtaaatt gaatatgctt ccaatcgaat gtattgttcg
     3181 tggttacatc acaggtagtg gatgggctag ctacaaagaa acaggaaagg tttgtggtat
     3241 tgaacttcca gaagggttac aagaatcaca acaacttcca gaaccaattt atacacctag
     3301 tacaaaggct gaaattggtg atcacgatga aaatatttct tttgaacaaa gtattgcgca
     3361 cttagaaaaa cacttcccag gacatggtga agaatatgca actaagatca aagattgcac
     3421 aatcgcttta tataaaaaat gtgctgagta tgctttaagc aaaggaatta ttattgcgga
     3481 tacaaaattt gaatttggtt tagacgaaaa tggtaatatc gttatcggcg atgaaatgtt
     3541 aactcctgat agttctcgtt tctggcctgc agacggatat gaagctggtc attcgcaacc
     3601 atcatttgac aaacaatttg cgcgtgactg gctaaaggca aatccagata atgactggac
     3661 acttcctcaa gagattgtgg ataagacaat tgaaaaatat cttcaagctt acacaatgtt
     3721 gacaggaaaa acattataat tgaaagatac ccataagtgg aatgcttatg ggtgtttttt
     3781 tgaaaactgt attttttgat gcctaaatat atttgaaaac cgtaaaatat gatgttaata
     3841 atcacttgaa aaccgaagaa cgctatgttt aaatgaggga tagatattag aaaataataa
     3901 ttattgaata tatatggtga ggaaaaatat gaaacaaggt gtatttataa catttgaagg
     3961 aaatgatgga tcaggaaaga caactgtatg taaagcagta caagaagaat taatcaaaat
     4021 gggatacgat tgtatctata caagagaacc cggaggaagt aagattgccg aatctattcg
     4081 taatatatta ttagatgtaa acaatacgga gatggacaat cgtacagaag cattattata
     4141 tgcagcaagt cgtagacaac atttaaaaga agttgttctt ccggcattgg aaaataagaa
     4201 gattgtattg tgtgatcgct tcattgattc tagtttggca taccaaggaa ttgcacgagg
     4261 tattggttta aaagaagtgt gggatatcaa tcagtttgcg attgatggat gtatgcctga
     4321 aaagactcta tttttatccg ttagtgtaga aacgggtaaa aaacgtatga atcttcgtgg
     4381 cgacttgaat cgtttggacc aagaagaaag ttcattccac aaagctgtac gtcaaggcta
     4441 tgaaacatta gtttctatgt atccagaacg cattgaagtg attgatgccg agccttgtaa
     4501 agaagaagtt ttaaaaagtg ctatggaatg tgttttaaag gttattaaag aacatgaata
     4561 aagagtattt aaaaaaagca caacctgttg tgtatcagac gttgtcgaat gctttaaaga
     4621 atcaaagagt tgcgaatgcc tatttatttc acgggccaaa aggctcaagc aaattagaaa
     4681 cggccatctt atttgcacaa agtattgtat gtgaacatac agataatgat ggatttgcat
     4741 gccaagaatg tgaaagttgt aaacgtatag agaacgaaga atctttagac tacatgattg
     4801 tatcgaatga tcgtattaaa aagaaagata tagtggattt acagacgttt tttgaatcaa
     4861 cgagtgcagg aaaacaaaat gctcgaatct atattctaga tcattatgat aaggctacgc
     4921 cagatgcttc aaatagcttg ttgaagtttt tagaggagcc aagtgaaggg atttatggta
     4981 tattaattgc ggatgaaaaa tccaatgtat taccgactat tcaatctagg tgtcaggcta
     5041 ttcattttaa accggaaagc tgtgaacact tattgcttga aaatacaagt gaagaaaacg
     5101 caaagatgct tgcagaatct ggatatacat atgataaggc aattgaatta ttgtctttac
     5161 ctgattttga agaattaaaa caaattgcac ttgaatatat tgaacatgcc tcttcttttt
     5221 cacaaattga aaagatgcag acaaatatat ttgtgaataa atcagatcgt atgaataagg
     5281 attgggtaca gttgtggatt cagtgggttt tattctatgt aaaaaatgat agggtaaatt
     5341 tacctttaga aaaaaaagta aaactatatt cgattttggt tgaaaatatg gatatgttgt
     5401 atcgacctgt cgatttaggt ttatttatgg ataagcttta ttatgatatt aggaaggctg
     5461 tgatttagtg aatacagaag gaaaagtaac atataaatat attgtttata tacagtttga
     5521 agaatcaaaa aaagcctaca cttttggatc agatgttaaa tattatacaa atgatgttgt
     5581 cgttgtagaa actgtacgtg ggcaagaact aggaaaggtt tgtgtgccaa cggttgattt
     5641 tgatgctttg aaagtaaagg gagatattaa accagtattg cgtaaggcaa cgcaagaaga
     5701 tattaaatgt aaagaagaaa atgttgaaaa agctaaagaa gcaatgaaga tttgtcacga
     5761 gtgtgtcgca aatttgaagt tggatatgca tttaatcagc tcagaatata cattagatcg
     5821 tacaaaagtc atttttacat atgtgtcgga cgatcgtgtt gattttagac aactattgaa
     5881 agacttagct caacatttac attgtcgtat cgagttaaga caagtagggc caagaaacaa
     5941 agctaaaatc gtaggtggaa ttggaaattg cggtatggaa tgttgctgct caagatttat
     6001 gtcagatttt gatacggtat ctataaatat ggcaaaaaat caaatgttgg cattgaatat
     6061 tcagaaattg agtggtcaat gtggaaagct aatgtgttgt ttacgtttcg aaaatgaaga
     6121 gtatacaaga atgcgtaaag atttaccaaa gatgaattcg attgtgagtt atcaaggtaa
     6181 aaaatatcgt atttcaagta tgaatgtgct tcaaaaacag gcaaaactag aaaacaaaga
     6241 agaagtcttg tttgtggact ttaaagaatt gtggccagat aaggatttta acaatgatta
     6301 gacagaagag ttttgaatcg aatacttcaa cattgtattt ggttcctacg cctattggca
     6361 atttacagga aatgacacct agagctctag atattttaaa agaagtgagt gtgattgcgg
     6421 cagaagatac gcgaaataca aaaaaacttt taatggcata tggcattgat acaatgttga
     6481 tgtcgcatca tgaacataat cagcaagtta gtattccaaa aatcattgaa cgattggaaa
     6541 atggtgaaga tgttgcggta gtatccgatg caggttatcc cttggtttcg gatccagggc
     6601 aaaaattagt tcaagaaacc atttcgcatg gatttaatgt ggtttcagtt tcaggtgcca
     6661 acgcggctat gaatgcattg gttgcgagtg gattgagttg ttatcattat ttattctatg
     6721 gctttttaga caataagagt tcaaaaagaa agaaacaact cgaagaaatc aaaacaattc
     6781 cttatacaat gattttttat gaagcacccc atcgtattga agatactcta caagatatgt
     6841 tggaagtatt gggaaatcgt caaatgtgtt tagctagaga actcacaaaa gtgcatgaag
     6901 aatatattcg tggaaccatt gaagaagtgt tagaggtagc gactacttgt aaaggtgaaa
     6961 tggttattgt catggatgga tttaaacaag atgaagttgt atttgatatg tatacagcca
     7021 ttacgaaagt ggatgaatat attgaatctg gaatgcgtac aaaagaagcg attggtttag
     7081 ttgccaaaga aatggcttgt aaaaagaatg aattatatga agcttatcat aaaagatagg
     7141 cttttttctt ttatataaag caaataacct taactttgta aaaaatgtat aactacaatt
     7201 gaatttttaa atgaatagtt gaaatttcct gtatttatgg acgatttcaa cttttt
//
LOCUS       DBMDWL010000108         7219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_3871_length_7219_cov_3.343074, whole genome shotgun sequence.
ACCESSION   DBMDWL010000108 DBMDWL010000000
VERSION     DBMDWL010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_7219_cov_3.343074"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="ACZPDN_07890"
     CDS             <1..1027
                     /locus_tag="ACZPDN_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1298583.1"
                     /translation="SELLSKMLTKKKIPHEVLNAKNHAREAEIIAKAGRPKSVTIATN
                     MAGRGTDIKLTEESRKLGGLAVLGSERHESRRIDNQLRGRSGRQGDPGFSRFYVSLED
                     SLMVRFGGDKLQKLFEKMGDEQIESKAVTKSITMAQKRVEGYNFDMRKQLLDYDDVLR
                     RQREIIYAQRNRILEADEVHDMVHVVFEKTIEQTLQANLSDQKKQTIDVAGVVKSIEM
                     MGMAEDKAIRASELEGKSYDEVKEYCVNKIWNEYEAEIKDVKRQFLPFEKTVVLRNID
                     RNWINHIDMMDKLRSGIYLRSYAQNNPLQQYVQEGFDMFEEMNQRIDREITFFLLKVR
                     IRKEHAA"
     gene            1027..2128
                     /gene="prfB"
                     /locus_tag="ACZPDN_07895"
     CDS             join(1027..1098,1100..2128)
                     /gene="prfB"
                     /locus_tag="ACZPDN_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143395314.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR1298584.1"
                     /translation="MELYELKQGVSTHLEKLHSLGESLDLSSKNKQIEDYDKQTLEPD
                     FWQDQKKAQQVIRAKNTLKDIVDRYEELDLQLTSLNDTADELKSEFDEELMMIAEEEY
                     MDMDKKFENFEIQVLLSHEYDQNNAILEIHPGAGGTESCDWASMLYRMYTRYAEKHGF
                     KVSVLDYLPGDEAGIKSVTFLVEGDKAYGYLKSEKGVHRLVRISPFDSGARRHTSFAS
                     LDVMPQFNDEIEIDIKPEDLIVETKRASGAGGQHINKTDSAVRMVHKPTGIVATCQNG
                     RSQHENREECLRVLKSRLYQLEIEEQEKKIAQIKGVQSANEWGSQIRSYVMHPYSLVK
                     DVRTGYETSQVQSVLDGDLDEFIFAYLKSQIQ"
     gene            2237..3124
                     /locus_tag="ACZPDN_07900"
     CDS             2237..3124
                     /locus_tag="ACZPDN_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298585.1"
                     /translation="MNVYYHLPGLFEFYEFYKKFLPLFKNKPEYFYDWCKIGSIYGSP
                     SDCIWSGGRISYADCDPKKVFALMKEYNISSRLTFSNSLIEEKHLSDIKCNELCRLLN
                     LDTNNGIIIHSDILMKYLKKKYPNLYFASSTTKVLTNFNDFKKEVENTDFTYVVPDFR
                     LNKQLEKLNSLSESYKPKVEFLCNECCWYGCKDRKECYKSVSKQNLGIDCMDHVCKAP
                     FSKEGYRFSRVMENPAFISLEDILNIYVPMGYSNFKIEGRDLGSALLLEFILYYMVKP
                     KYQIHVREAMYLDAMLDLF"
     gene            3136..3804
                     /locus_tag="ACZPDN_07905"
     CDS             3136..3804
                     /locus_tag="ACZPDN_07905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1298586.1"
                     /translation="MMIEKRHTVRKYLDQPLDMALVDLLNDRIKQNNKLYNLTFKLVT
                     NKSDGLSSLAKIMSNNTVQNYIVLAGKECSSLDEKIGYCGADLILYAQSLGLNTWWCG
                     GMLNGKNALKHLDDKDVRVNGVIVIGYGKIQGVPHKSKTADQVSHYKGKAPEWFNSGI
                     KALLLAPSALNRQPYIVTGAGNKVIFKVKSGFLSQVDLGIGKYFFELGAGKENFEWSS
                     YDRN"
     gene            3791..4453
                     /locus_tag="ACZPDN_07910"
     CDS             3791..4453
                     /locus_tag="ACZPDN_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR1298587.1"
                     /translation="MIEIENKIFLKKKFDFEKLKEFGFSFENNIYVYTEDFLNSNFKA
                     VIEISDVLKGKVIDNLSNEEYMPLRIESFDGDYVCSVRKAYKDILKRIVDICCTDVFF
                     ADDQANRITKFIDETYGVKPDFPWKDDNGVFRHLDNNKWFSLIMYVSLDALLKNGDNR
                     KLNIINLKIDTSKRDELYEIDGIYPAFHMNHKMWISVVLDDTISDEKVMELVTCSFDL
                     TK"
     gene            4466..5425
                     /locus_tag="ACZPDN_07915"
     CDS             4466..5425
                     /locus_tag="ACZPDN_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HIR1298588.1"
                     /translation="MYGIIATWRMALEGISKADSMLKNNESAANSIEEAIKAVENFEY
                     YKSVGYGGLPNEEMEVELDAAFMDGDTLEFGCVGGVKDFANPVSIARSLMHYDANNFL
                     AGQGAEKYASRHGFERKTMLTDRAKIHYHNRIKEITNTELKPYSGHDTVGMVCLDTAG
                     HMTCATSTSGLFMKHPGRLGDSCVCGSGFYVDSLVGGASATGLGEDVMKGCVSYEIVR
                     LMKEGMHPQQACEKAVHDFDLELKRRRGKAGDMSLIAMNNKGEWGCATNIEGFSFVVA
                     TEKQAPTVHLVNRDADGKCTFEVASKEWMDNYMALRTAPLVRK"
     gene            5427..6548
                     /locus_tag="ACZPDN_07920"
     CDS             5427..6548
                     /locus_tag="ACZPDN_07920"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HIR1298589.1"
                     /translation="MEEELLKKIDEISDEMISGIKRIAQIESVQSEAKLGMPFGEGVN
                     KALEEALKLARELGFETENVDHMVGIARYGTGEDYIGIMGHLDVVPVGEGWNHPPFSA
                     YEDEKGRIYSRGILDNKGPTLSCLYALYAIKKLGIQLKRPVHILFGTNEETGFEDLKH
                     FLKVRKPPIMGWTPDCKYPVVYAERGRSTYRVSTAIENKTVFNQFINEYILSDNGFGN
                     KLGLNIEDLEFGKMQMSNKKLVDLEGKLGFDFSFSYPASIRNDTIERTIKSKLSKGLQ
                     VECIHNYDPVYFDKNGFLCKTLKATYEKVTGMDGTPVTTTGGTYAKIMPNIVPFGPSF
                     PGQKGIGHNPNEWMDRSDLILNAKIYALSIVRLANGEND"
     gene            6532..>7219
                     /locus_tag="ACZPDN_07925"
     CDS             6532..>7219
                     /locus_tag="ACZPDN_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIR1298590.1"
                     /translation="MERMIEVCAGSYQDCIAAYQGSAKRVELNSALSVGGLTPSVASL
                     IRVKKETDLTVICMVRVRAAGFCYEKEDVEIMFEDAKILLEHGADGIAFGFLNEDGTI
                     CEEDTKRMIDLIHSYNKEAVFHRAFDVTPDAFEAIETLIDLGCDRVLTSGQKSKAMEG
                     IDLIKVLNNQYGEKIQILPGSGMNAENAKMMMNYTGVYQVHSSCKNYKTDTTTTKNGV
                     SYSYLASPHEN"
BASE COUNT         2549 a          930 c         1534 g         2206 t
ORIGIN      
        1 ttctgaacta cttagtaaaa tgcttactaa aaagaagata cctcatgaag tgttaaatgc
       61 gaagaaccat gctcgtgaag cagaaatcat cgcaaaggca ggtcgtccaa aatcagttac
      121 aattgcgaca aacatggctg gtcgtggtac cgatatcaaa ttaacagaag aatcacgtaa
      181 attaggtggt ttagctgtat tgggtagtga aagacatgaa tctcgtcgta ttgataacca
      241 gttacgtgga cgttcaggtc gtcaaggaga tcctggattc tcacgtttct atgtatcttt
      301 agaagattca ttaatggttc gttttggtgg cgacaagcta caaaaactat ttgaaaaaat
      361 gggtgacgaa caaattgagt caaaggcagt cacaaaatca atcacaatgg ctcaaaaacg
      421 tgttgaagga tataactttg atatgcgtaa acaattatta gattatgatg atgtattgcg
      481 tcgccaacgt gaaattattt atgcacagcg aaatcgtatc ttagaagcag atgaagtaca
      541 tgatatggtt catgttgtgt ttgagaaaac tattgaacag actttgcaag ctaatttatc
      601 agatcaaaag aaacaaacaa ttgatgttgc aggcgttgtt aaatccattg aaatgatggg
      661 tatggctgaa gataaggcga ttcgtgcaag tgaacttgaa ggaaagtctt atgatgaagt
      721 taaagaatat tgtgtaaata agatttggaa tgaatatgaa gctgagatca aagatgtgaa
      781 gcgtcaattc ttaccttttg aaaagacagt tgtattacgt aatattgacc gtaactggat
      841 caaccatatt gatatgatgg ataaattgcg tagtggtatc tatttgcgtt cttatgcaca
      901 aaacaatcct ctacaacaat atgtacaaga aggatttgat atgtttgaag aaatgaatca
      961 aagaattgat cgtgaaatca cattcttctt attaaaggtt cgtattcgta aggagcatgc
     1021 agcctaatgg aattatatga attgaaacaa ggagtgagta ctcacctaga gaaattacat
     1081 tctttaggtg agtcactttg acttatctag taaaaataaa caaatcgaag actatgataa
     1141 acaaacttta gaaccagatt tttggcaaga tcaaaaaaaa gctcaacaag tgattcgtgc
     1201 aaaaaataca ttaaaagata ttgtagatcg ttatgaagaa ttggatttac aattaacttc
     1261 gttaaatgat acggcagatg aattaaagtc tgaatttgat gaagaattga tgatgattgc
     1321 cgaagaagaa tatatggata tggataagaa gtttgagaac tttgaaatcc aagtcttgtt
     1381 aagtcatgaa tatgatcaaa acaatgcgat tctagaaatt cacccaggag ctggtggaac
     1441 cgagtcttgt gactgggcaa gtatgcttta tcgtatgtat acaagatatg ctgaaaaaca
     1501 tggctttaaa gtgagtgttc tagactattt acctggagat gaagctggta ttaaaagtgt
     1561 aactttcctt gtcgaaggag ataaagctta tggctatctg aaatcagaaa agggtgtaca
     1621 tcgtttagtt cgtatttcac catttgattc aggagctaga agacacactt cgtttgcgag
     1681 tttagatgtt atgcctcaat tcaatgatga aattgaaatt gacatcaagc cagaagattt
     1741 aattgttgaa acaaaacgtg cttctggtgc tggaggacaa catatcaata aaacggatag
     1801 tgccgtacgt atggtgcata agccaacggg tattgtggct acatgtcaaa atggacgtag
     1861 tcagcatgaa aatagagaag aatgtttgcg tgtcttaaaa tctcgtttgt atcaattaga
     1921 aattgaagaa caagaaaaga agattgctca aattaaaggt gtacaatccg ctaatgaatg
     1981 gggcagccaa attcgaagct atgtcatgca tccatatagc ttagtcaaag atgttcgtac
     2041 aggatatgaa acaagtcaag ttcaatctgt attagatgga gatcttgatg aattcatctt
     2101 tgcgtattta aaatcacaaa tccaataaaa tccaatcgaa aggttggatt ttttctatat
     2161 tcgctcataa aaatgtaatt attttaaatt attccctcat aaaagtgtaa aatactctta
     2221 tcaagaggtt aagaatatga atgtttatta tcatttaccg ggcttatttg aattctatga
     2281 attttataaa aagtttttgc ccctatttaa aaataaacca gaatacttct atgactggtg
     2341 taagattggc tctatctatg gctcaccatc tgattgtata tggtctggtg gacgcatcag
     2401 ttatgcagat tgtgatccaa aaaaagtatt cgctttgatg aaagaatata atatatcttc
     2461 acgtcttaca tttagtaatt ctttgattga agaaaaacat ttgtccgata ttaaatgcaa
     2521 tgaattatgt cgattgttga atttagatac aaataatgga attattattc attcggatat
     2581 attgatgaaa tatctgaaaa agaaatatcc gaatctatat tttgcatctt caaccacaaa
     2641 agtattaaca aattttaatg attttaaaaa ggaagtcgaa aacacagact ttacgtatgt
     2701 agtccctgat tttcggttga ataaacaatt agaaaagttg aactcactat ctgaatcata
     2761 taaaccaaag gtagaatttt tatgcaacga atgttgctgg tacggatgta aagatcgtaa
     2821 ggaatgttat aaatcggttt ctaaacaaaa tctaggcatt gattgtatgg atcatgtatg
     2881 taaggcccct ttttctaagg aagggtatcg tttttctaga gtgatggaaa atcctgcatt
     2941 tattagttta gaggatattt taaatatcta tgtccctatg ggctattcaa attttaaaat
     3001 cgaaggaaga gatttaggaa gtgctctatt attagaattt attctttact atatggtaaa
     3061 accaaaatac caaatccatg ttcgtgaagc aatgtattta gatgcaatgc tagacttgtt
     3121 ctaaaaggag agattatgat gatagaaaaa agacacacag tacgtaagta tttagatcaa
     3181 ccattagata tggctttggt tgatttgttg aatgatcgta ttaaacaaaa taataaattg
     3241 tataatttga catttaaact cgtgacaaat aaatcagatg gactttctag tttagctaaa
     3301 attatgtcga ataataccgt acaaaactat atcgttcttg caggcaaaga atgttcaagt
     3361 ttagatgaaa agataggcta ttgtggtgct gacttgattt tgtatgccca aagtttaggc
     3421 ttaaatacat ggtggtgtgg aggtatgctt aatggcaaga atgctcttaa acatttagat
     3481 gataaagatg ttagagtgaa tggtgtcatt gtgattggat atggaaaaat acagggggta
     3541 ccacacaaat caaagacggc tgatcaagtg agccattata aaggaaaagc tccggaatgg
     3601 tttaattcag gtatcaaagc tttattatta gcacctagtg ctttaaatag acaaccatat
     3661 atagtgactg gtgcgggtaa taaagtaatt tttaaagtaa aaagtggatt tttatcacaa
     3721 gtcgatttag gcataggaaa atatttcttt gaacttggtg caggaaaaga aaattttgaa
     3781 tggagttcat atgatagaaa ttgagaataa aatcttttta aaaaagaaat ttgattttga
     3841 aaaattgaaa gaatttggtt tttcttttga gaataacata tatgtttata cagaagattt
     3901 tttgaattca aattttaaag ctgtcattga aatatcggat gttttaaaag gtaaagtcat
     3961 tgataattta agtaatgaag aatatatgcc actgcgcata gaatcctttg atggggatta
     4021 tgtttgtagt gttcgcaaag cttataaaga catattaaaa agaattgtgg atatatgttg
     4081 tacagatgta ttctttgcgg atgatcaagc gaatcgtatt acgaaattca ttgatgaaac
     4141 gtatggagta aaaccagatt ttccatggaa ggatgacaat ggtgtttttc gtcatttaga
     4201 caataataaa tggttttccc ttatcatgta tgtttcgtta gatgctctat taaaaaatgg
     4261 agataataga aagttgaata ttatcaattt aaagatagat acttctaaaa gagatgaact
     4321 ttatgaaata gacggtattt atccggcctt tcatatgaat cataaaatgt ggatatctgt
     4381 cgtacttgac gatactataa gtgatgaaaa agtaatggaa cttgtaacgt gtagttttga
     4441 tttaacaaaa taaggagaga atgatatgta cggaattatc gcaacatgga gaatggcttt
     4501 agaaggaatt tcaaaagcag attctatgtt aaaaaataac gaaagtgcgg cgaattcgat
     4561 tgaagaagca atcaaagctg tagagaattt tgaatactat aaaagtgtag gatatggtgg
     4621 acttccaaat gaagaaatgg aagtcgaatt agatgctgct tttatggatg gagatacact
     4681 agaatttgga tgtgttggag gcgttaaaga ttttgccaac cctgttagta ttgcacgtag
     4741 tctaatgcac tatgatgcca acaatttctt agcaggtcaa ggagctgaaa aatatgcttc
     4801 aagacatggc tttgaaagaa aaacaatgct tacagatcgt gctaagattc attatcacaa
     4861 tcgtataaaa gaaatcacaa atacggaatt aaaaccatat tcaggacatg acacagtagg
     4921 tatggtttgt ctagatactg caggacacat gacttgtgct acaagtactt ctggattgtt
     4981 catgaaacat ccaggacgtc ttggcgattc ttgtgtatgt ggttctggat tttatgtaga
     5041 ttctttggtc ggaggagcga gtgctacagg tttaggtgaa gatgttatga agggctgtgt
     5101 ttcttatgaa attgttcgtc taatgaaaga aggcatgcat cctcaacaag cgtgtgaaaa
     5161 agctgtacac gattttgatt tggaattaaa acgaagaaga ggcaaagctg gagacatgtc
     5221 tttgattgcg atgaataata aaggtgaatg gggttgtgct acaaatattg aaggttttag
     5281 ttttgtggtg gcaactgaaa agcaagcacc aacggtacat ttagtcaatc gtgacgcgga
     5341 cggaaaatgc acatttgaag tcgcaagtaa agaatggatg gacaactata tggctttacg
     5401 tacagctcca ttagttagga aataatatgg aagaagaatt gttaaagaag attgatgaaa
     5461 tcagtgatga gatgatttca gggatcaaaa gaattgctca aatcgagagt gtgcaaagtg
     5521 aagctaagtt aggtatgcct tttggtgaag gcgtaaataa agctttagaa gaagcattga
     5581 aattagctcg tgaattaggt tttgaaacag aaaacgtcga tcatatggtg ggcattgcca
     5641 gatatggtac gggtgaagat tatattggta ttatggggca tttagatgtt gttcctgtag
     5701 gagaaggatg gaatcatcct ccattctcag cttatgaaga tgaaaagggt agaatttatt
     5761 caagaggaat cttagataat aaagggccga ctttatcttg tctatacgca ttatatgcca
     5821 taaaaaaatt gggcattcaa ttaaagcgtc ctgttcatat tttgtttgga acaaatgaag
     5881 aaacgggatt tgaagattta aaacattttc ttaaggtcag aaaaccacct attatggggt
     5941 ggacacccga ttgtaaatat ccggttgtat atgcggaaag aggaagaagt acctatcgtg
     6001 tgagtacggc tattgaaaat aaaactgtat tcaatcaatt tattaatgaa tatattttat
     6061 cagacaatgg atttggaaat aaattgggac tcaatattga ggatcttgaa tttggtaaaa
     6121 tgcagatgag caataaaaaa ttggtggatt tagaaggaaa acttggcttt gatttctctt
     6181 ttagctatcc tgcaagtatt aggaatgata caatagagag gacaatcaaa tctaaattaa
     6241 gcaaaggact acaagtagaa tgtatacata attatgatcc tgtttatttt gataaaaatg
     6301 ggttcttatg taaaactcta aaggcgactt atgaaaaggt aacaggcatg gatggtacgc
     6361 cagttacaac tacgggtgga acgtatgcga agatcatgcc aaatatagtt ccttttggac
     6421 cgagttttcc tggacaaaag ggcattggac acaatccgaa tgaatggatg gatcggagtg
     6481 atttgatttt aaatgcgaaa atatacgcat taagtatagt gaggttggca aatggagaga
     6541 atgattgaag tatgtgcagg tagctatcaa gattgtatag ctgcatatca aggtagtgca
     6601 aagagggttg aattaaacag tgcgttaagt gttggtggct taacgccaag tgtcgcaagt
     6661 ctaattcgtg tgaaaaaaga aacagactta accgtaattt gtatggtacg tgtgcgtgct
     6721 gcaggtttct gttatgaaaa agaagatgta gaaattatgt ttgaagatgc caagattctt
     6781 ttagagcatg gagctgatgg aattgcgttt ggtttcttaa atgaggatgg cacaatttgc
     6841 gaagaggaca caaaaagaat gatcgacttg attcattcat acaataagga agctgtattc
     6901 cacagagctt ttgatgttac gccagatgca tttgaagcca ttgaaacatt gattgattta
     6961 ggttgtgatc gcgtacttac aagtggacaa aaatcaaaag ctatggaagg tattgatttg
     7021 attaaagtat tgaataatca atatggagaa aagattcaaa ttcttcctgg tagtggaatg
     7081 aatgcagaaa acgcaaaaat gatgatgaat tatacaggtg tatatcaggt gcattcgtct
     7141 tgtaaaaact acaaaacaga tacaaccaca acaaaaaacg gtgtttcata tagttacttg
     7201 gctagtccac atgaaaatg
//
LOCUS       DBMDWL010000109         7086 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4004_length_7086_cov_2.397566, whole genome shotgun sequence.
ACCESSION   DBMDWL010000109 DBMDWL010000000
VERSION     DBMDWL010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_7086_cov_2.397566"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="ACZPDN_07930"
     CDS             <1..894
                     /locus_tag="ACZPDN_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HIR1298591.1"
                     /translation="VGLGGAGFPTYVKYMKPDGVDYVLINAVECEPYITADYRSVMDD
                     AQDLVTGAKIMKKMAEAKTVYICIKESHPDLIEKVKSALTDAAGVEVYPVPDVYPMGW
                     ERVLVREVLHKEYGGLPAEAGAIIGNASSAIAIAKAFEKGEAITQKNLTVSGECVKDP
                     HNVCVPVGMPVSEIIQACGGYTQDTVRLISGGPMMGKTIVNDMFVVDRAMNALTVLPA
                     ANDDAIACLRCGKCSDHCPSGLQPVRITAALKANDVDEMSKRGVMSCIECGMCTYICP
                     SHIDVTENVRKAKRIVMLKKK"
     gene            906..2048
                     /locus_tag="ACZPDN_07935"
     CDS             906..2048
                     /locus_tag="ACZPDN_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR1298592.1"
                     /translation="MKFTFHVSPSIKNNLSTQRIMKELSLSLLVVFVAAVIYYASAWG
                     TDAALHCVLLLACSLITTFVCESIFAKIMKKDIKTFLKSSFGWVTAIILTLMVPVNMS
                     CYAVIIATVFAIVLAKLLFGGFGQNIFNPAAVGRAVILQAFTGVSVGLISQATPTTVI
                     ASAYHWLPANATVLDSFLSQYGGFAGLALGTYPGSIGETFSILILIIGVVLAVRQIID
                     WRVPVVYLGTIFVLTAVVALITGIDSYNGIPAFIWYPLLHLLTGGVIFGAVFMLTDPV
                     TSPTSASGRVVFAMGAAIITTLIRLKANLPEGCLYSILLMNMFTPWIESCFDGKQLEL
                     VKKSRIVCACLALIGLAISFYAASSIQKVDTIPETAAVVETMEVTL"
     gene            2045..2554
                     /locus_tag="ACZPDN_07940"
     CDS             2045..2554
                     /locus_tag="ACZPDN_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1298593.1"
                     /translation="MNKIVHLGLFLAIVSAVAGGALAFANQMTAPVIAENNERQEKES
                     LLAMYPDANSNDFKLVNKTLDSKTVQKVYEYKNVYIFNMSVSGYKDGTVFLVSIDKDS
                     KKIDKYNAISNGDTKGLGSQVTEAPFKKSLEGKDATGQLDTISGATVSSGPVVSGIHE
                     AAKLVDQLK"
     gene            2568..3227
                     /gene="rsxE"
                     /locus_tag="ACZPDN_07945"
     CDS             2568..3227
                     /gene="rsxE"
                     /locus_tag="ACZPDN_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR1298594.1"
                     /translation="MNRFENFKAGLIKDNPVFSLFLGICSTLAITTTVNNAIGMGVSV
                     IIVLIMSNVIISCVRKITPDEIRIPVYIVIIATLVKIIQMLIQAYAPSLNTSLGVFIP
                     LIVVNCIILGRAEAFASKNGILDSALDGLGMGLGYTLAVLSMSFIRELISTGGIKVVN
                     PFNEAQVWLNLHIIPENFTISIFGSSVGAFITFAVLAAAVGAYKQHVTAKEEAATKEE
                     K"
     gene            3229..3804
                     /locus_tag="ACZPDN_07950"
     CDS             3229..3804
                     /locus_tag="ACZPDN_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HIR1298595.1"
                     /translation="MGELMALLVTSVLVNNVILTQFLGMCPFMGVSKKKSSAIGMGVA
                     VVFVIVVAALVTYALYYLVLVPLQLEFMDLITFILVIASLVQLTEMFIKKTSPALYKS
                     LGVYLPLITTNCVVLNVCLVNISNSYNFAQMLVYSIGTPLGFALVLFIFSTIRERLEQ
                     SEVPNAFVGNPIALIVAAIMAMAFSCFTGIV"
     gene            3804..3986
                     /locus_tag="ACZPDN_07955"
     CDS             3804..3986
                     /locus_tag="ACZPDN_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298596.1"
                     /translation="MRYLLAILFFGALVAIYIVLYLMNKKTPKPEGCEDLKADCDGCK
                     DFACTNNPAHNQVKGE"
     gene            3989..4297
                     /locus_tag="ACZPDN_07960"
     CDS             3989..4297
                     /locus_tag="ACZPDN_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR1298597.1"
                     /translation="MVIVAVIMMLVLGAVLGLGLGVADKYLKVEADVRVEKVTSLLPG
                     YNCGGCGFPGCSGMAEALVSGDMDHIACKPCKADKREEIIDYLKNTPGPDGSTIEVKG
                     "
     gene            complement(4325..5959)
                     /locus_tag="ACZPDN_07965"
     CDS             complement(4325..5959)
                     /locus_tag="ACZPDN_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990112 - RQC complex [Evidence IEA];
                     GO_function: GO:0001968 - fibronectin binding [Evidence
                     IEA];
                     GO_process: GO:0072344 - rescue of stalled ribosome
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein"
                     /protein_id="HIR1298598.1"
                     /translation="MAIDGLFLYNIQEKLNAYTPCKIGKIQNISDEEILIHLHTRNDG
                     NQKLVINVHSNTNRVYIANFIPGIQPEPSNFVMVLRKLCSQAIVESFEQVGYDRILCL
                     HLSCRNEFQDLVKYDLNIELMGKYANLILVKEGVIVDALKRIPVYENAKRFIHPGAKY
                     VAPTQPEKKNPFEVQDLDLDQSLVKQIYGFSPLLSNEFLYRMNNNEKYIDILNEVVNS
                     NTLYIYKKDFHCIELKHLNEQAKTYTIMEGLNHLYEEDEQKKRIKEQYGDIIRMVDKE
                     KNKQIKKLPKLEQALEDGKDYHKYQEYGDLIFAYMHQIKKEKTIILPSFETNEDVTIP
                     IDMRYDLKTNANKYYQKYHKMKLSLVILEEQINQCKEDIEYYTQLEEQLQHCSVFDAG
                     EIREELIKQRVLMPKKENKRKKKNNKPNVLHLVFDDCEIYVGKNNIQNNYVTHQVSRK
                     NDLWFHVKDYHGSHVLLKSDDFTETQIRLCAMLAAYYSEGKDSSSVPVDYCLISQIKK
                     VPGSKIGFVTMKSNKTIYIDPDETYLLEIIKNHQVK"
     gene            6065..6643
                     /gene="gmk"
                     /locus_tag="ACZPDN_07970"
     CDS             6065..6643
                     /gene="gmk"
                     /locus_tag="ACZPDN_07970"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1298599.1"
                     /translation="MERGLLIILSGPSGVGKGTIRKYFEKDERLNLAYSTSMTTRKPR
                     AGEVDGKDYFFSTREDFEKAIQEGGLLEWAEFVGNYYGTPLKEVNRLRDEGKNVLLEI
                     EVQGAIQVQEKCPDAVSIFIIPPSMEELERRIRGRSTEPEEIIQQRLAKASNEMKMVS
                     QYKYIVCNDDPKLAAELIKTIILRHVELEENK"
     gene            6738..>7086
                     /locus_tag="ACZPDN_07975"
     CDS             6738..>7086
                     /locus_tag="ACZPDN_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR1298600.1"
                     /translation="MDNLKEWIEEVLNENGCHLYDLEWITNQGTPLLRVSVEKEDGTM
                     DLDTCASCSDVIGQMLDEKDWYSHEYMLEVCSPGAERELRTDEEVQRAVGKYVFVKMK
                     DPKQGLDQVIGDLV"
BASE COUNT         2166 a         1025 c         1425 g         2470 t
ORIGIN      
        1 gttggtttag gtggagctgg ttttccaacc tatgtaaaat atatgaaacc agatggagtt
       61 gattatgtat tgatcaatgc cgtagaatgt gagccttata ttacagccga ttaccgctct
      121 gttatggatg atgcccaaga tttagttaca ggtgctaaaa tcatgaagaa gatggctgaa
      181 gctaaaacgg tttacatttg tatcaaagaa tctcacccgg atttaattga aaaagtgaaa
      241 tctgcattga cagatgcggc tggtgttgag gtatatccag tccctgatgt ttacccaatg
      301 ggttgggaac gtgttttagt acgtgaagta ttacataaag aatatggtgg attacctgct
      361 gaagctggtg ctatcattgg aaatgcttca agtgccattg cgattgcgaa agcctttgaa
      421 aaaggggaag ccatcacaca aaagaattta acagtgagtg gtgaatgtgt aaaagatcca
      481 cacaatgtat gtgtaccagt tggtatgcct gtaagtgaga tcattcaagc ttgtggtgga
      541 tatacacaag atacagttcg cttgatttct ggtggaccta tgatgggtaa aaccattgtc
      601 aatgatatgt ttgtcgttga tcgtgctatg aatgcattaa ctgtacttcc agctgcaaat
      661 gatgatgcga tcgcctgttt acgttgtggt aaatgtagtg atcattgtcc aagtggttta
      721 cagcctgtaa gaattacagc tgcattgaag gcaaatgacg tggacgaaat gtcaaaacgt
      781 ggtgttatga gttgtatcga atgtggaatg tgtacttata tttgtccttc tcatatcgat
      841 gtaactgaaa atgttcgtaa ggcaaaacgt attgttatgc ttaagaaaaa gtagggagga
      901 ttcaaatgaa atttactttt catgtaagtc caagcattaa gaataacttg tcaacacaac
      961 gtatcatgaa agaattatct ttaagtttat tagttgtgtt tgtagcagcc gtaatttact
     1021 atgcatctgc atggggtact gatgctgctt tacattgtgt tcttttatta gcttgttctt
     1081 taattactac atttgtttgc gaatccatct tcgcaaagat tatgaagaaa gatattaaaa
     1141 ctttcttaaa gagctcattt ggatgggtaa cagctatcat tttaactttg atggtacctg
     1201 ttaacatgtc ttgttatgca gttattattg cgacagtatt cgcaattgta cttgcaaaat
     1261 tattatttgg tggttttgga caaaatatct ttaaccctgc agctgtaggt cgtgctgtta
     1321 ttttacaggc atttacaggt gtgagtgtag gacttatttc acaagcaaca ccaactacag
     1381 tgattgcgag tgcttatcat tggctaccag caaatgctac tgtattagat tcatttttat
     1441 cacaatatgg aggatttgca ggtctagctt taggaacata tcctggttct attggtgaaa
     1501 cattctctat cttaattttg attattggtg ttgtgcttgc ggttcgtcaa atcattgact
     1561 ggagagttcc cgttgtctat ttaggaacga tttttgtact aacggctgtt gtagctttga
     1621 ttacaggaat tgattcatac aatggtattc cagcctttat ttggtatcca ttattacact
     1681 tgttaacggg tggtgttatt tttggtgctg tatttatgtt aacagatcca gtaaccagtc
     1741 caacaagtgc aagtggacgt gttgtatttg cgatgggtgc agctattata actacattga
     1801 ttcgtttaaa agccaatctt ccagaaggat gtttatattc catcttattg atgaatatgt
     1861 ttactccatg gatcgagtca tgtttcgatg gtaaacaatt agaattggtt aagaaatcta
     1921 gaattgtttg tgcttgttta gcattgattg gtttagctat tagtttctat gcagctagct
     1981 ctattcaaaa ggtagatact atccctgaaa cagcagctgt tgttgaaacg atggaggtaa
     2041 ctctatgaac aagattgtac atttaggttt attcttggct attgtaagcg ctgttgctgg
     2101 tggtgcctta gcattcgcaa atcaaatgac agctccagtt attgctgaaa ataacgaaag
     2161 acaagaaaaa gaatcattat tagctatgta tccagatgca aattcaaatg attttaaatt
     2221 agtgaataaa actttggatt caaaaaccgt tcaaaaggtt tatgaatata aaaatgttta
     2281 tatttttaac atgagtgtta gtggatataa ggatggaact gtattcttag tatctattga
     2341 taaagattca aagaaaattg ataaatacaa tgccattagt aatggagata caaaaggtct
     2401 aggatctcaa gtaacggaag ctccatttaa gaaatcttta gaaggaaaag atgcaactgg
     2461 acaattggat acaatttctg gtgcaactgt ttcttctggt cctgttgtta gtggtattca
     2521 tgaagcggca aaacttgtcg atcaattaaa gtaaggaggt tatctagatg aatcgttttg
     2581 aaaactttaa agcgggttta attaaagata acccagtatt ctcattattc ttaggaattt
     2641 gttcgacact agccattact acaactgtaa ataatgcgat tggtatgggt gttagtgtta
     2701 ttatcgtttt gatcatgtca aacgtaatca tttcatgtgt tcgtaaaatt acgccagatg
     2761 aaattcgtat tccagtttat attgttatta tcgcaacttt ggttaagatc attcaaatgt
     2821 tgattcaagc ctatgcgcca agtttaaata caagcttagg tgtatttatt ccattgatcg
     2881 tagttaactg tatcatcttg ggtcgtgcgg aagctttcgc tagtaaaaac ggaattttgg
     2941 attcagctct tgatggttta ggaatgggtc taggatatac tttagcagtg ctttcgatgt
     3001 catttattcg tgaattgatt tcgacaggtg gaattaaagt ggtaaaccca ttcaatgaag
     3061 cgcaagtttg gttgaactta cacattattc ctgaaaactt cacaattagt atctttggat
     3121 cgagtgttgg agctttcatt acatttgcgg ttttagcagc cgctgttggt gcttataaac
     3181 aacatgtaac agctaaggaa gaagctgcaa caaaggagga aaagtaagat gggtgaatta
     3241 atggcacttt tagtaacgag tgtattagta aataacgtta tcttaactca attcttaggt
     3301 atgtgtccat tcatgggtgt ttctaaaaag aaaagctcag caattggtat gggtgtggcc
     3361 gttgtcttcg ttatcgttgt tgctgcattg gttacatatg cactttatta tttagtactt
     3421 gtacctttac aacttgaatt tatggacttg attacattta tcttagtaat tgcctcttta
     3481 gttcagttga cagaaatgtt tattaaaaag acaagtcctg cgctttataa atctttaggt
     3541 gtttatttac ctttgatcac aacaaactgt gttgtattaa atgtatgttt agtaaatatc
     3601 tcaaatagtt ataattttgc acaaatgtta gtatactcaa ttggtactcc attaggattt
     3661 gcccttgtat tattcatttt ctcaacaatt cgtgaaagat tagagcaaag tgaagttcca
     3721 aatgccttcg ttggtaatcc aatcgccttg attgtagctg ccatcatggc tatggcattc
     3781 tcttgcttta caggaattgt ataatgagat atttattggc aatattgttt tttggcgcat
     3841 tagtcgctat ctatattgtt ttgtatttaa tgaataaaaa aacacccaaa ccagaggggt
     3901 gtgaagattt aaaagcggat tgtgatggat gtaaagattt tgcgtgcact aacaatccag
     3961 ctcacaatca agtgaaggga gaataaatat ggtaatcgta gccgtaatta tgatgcttgt
     4021 acttggtgca gtgcttggat taggtctagg tgttgccgac aaatatttaa aagtagaagc
     4081 agatgtacgt gttgaaaaag taacatcatt actacctggt tataactgtg gtggttgtgg
     4141 attccctgga tgtagcggta tggctgaagc cttggtttca ggtgatatgg atcatattgc
     4201 ttgtaaacct tgtaaagcgg ataagcgtga ggaaatcatt gattacttga aaaacacacc
     4261 aggaccggat ggttctacta ttgaagtaaa aggataaaaa aagagatgga attttccatc
     4321 tcttttactt tacttgatga tttttaatga tttctaataa ataagtttca tctggatcta
     4381 tatagattgt tttattggat ttcatggtca caaaaccaat tttagagcca ggtactttct
     4441 tgatttgaga aatcaaacaa tagtcaaccg gtacggaact tgaatcttta ccttcggagt
     4501 agtaggccgc caacattgca catagtcgaa tttgtgtttc tgtaaagtca tcactcttta
     4561 atagaacgtg agatccatga tagtctttta catgaaacca taaatcgttt tttcttgaga
     4621 cttggtgtgt tacataatta ttttgaatgt tgtttttacc gacatagatt tcacaatcat
     4681 caaaaacaag atgaagaacg ttgggtttat tgttcttctt ttttctttta ttttcttttt
     4741 taggcattaa aacacgttgc ttgatcaatt cctcacggat ttctcctgca tcaaatacgc
     4801 tacaatgctg taattgctct tcaagttgtg tatagtattc aatatcttct ttacattgat
     4861 tgatttgttc ttctaagatc actaaactta gtttcatctt atgatatttc tgatagtatt
     4921 tattcgcatt tgtttttaga tcataacgca tatcaattgg aattgttaca tcttcattgg
     4981 tttcaaacga aggtaagatg attgtctttt cttttttgat ttggtgcatg tacgcaaaaa
     5041 tcaaatcccc atattcttga tatttatgat agtctttacc atcttctaag gcttgttcaa
     5101 gctttggaag tttttttatt tgtttatttt tttctttatc gaccatacga ataatatcac
     5161 catattgttc tttaatacgt ttcttttgtt catcttcttc atataaatga tttaagcctt
     5221 ccatgattgt atatgtttta gcttgttcat ttaaatgttt taattcaatg caatgaaaat
     5281 cttttttata aatgtataaa gtatttgaat tcaccacttc atttaatatg tcgatatatt
     5341 tttcgttatt gttcatgcga tacaaaaatt catttgaaag taatggagaa aagccataga
     5401 tttgtttgac tagactttgg tctaaatcaa gatcttgaac ctcaaatgga tttttctttt
     5461 ctggttgggt aggagctaca tattttgcac caggatgaat gaatcgtttt gcattttcat
     5521 atacgggaat acgttttaat gcatctacaa ttacaccctc tttgactaaa ataagattgg
     5581 catatttacc catgagttcg atatttaaat cgtattttac taaatcttga aattcattac
     5641 gacaagataa atgtaaacat aaaatacgat catagccaac ttgttcaaag gattcgacaa
     5701 tggcttggga acataatttt cttaatacca tcacaaagtt ggatggttcg ggttgaatgc
     5761 ctggaataaa gtttgcgata tatacacgat ttgtattgga atgtacgttg atgacaagtt
     5821 tttgatttcc atcattacgc gtgtgaagat ggatcaagat ttcttcatcg ctaatatttt
     5881 gtattttacc aattttacat ggggtatatg cgtttaattt ttcttgaata ttgtataaaa
     5941 ataatccgtc gattgccata aaaacctcct tggtctgata aaaattataa catagttaaa
     6001 taaaatttga cacacatggt ttggacttgt aaaatggatg gaaaagaggg agagcaagat
     6061 tcacatggaa agaggtctat taattatttt gagtggtcct agtggtgtag gtaaagggac
     6121 aattcgtaaa tattttgaaa aagatgaacg tctaaattta gcttattcaa cttcaatgac
     6181 tacacgtaaa ccgcgtgctg gagaggtcga tggaaaagat tattttttct caacacgaga
     6241 agactttgag aaagccattc aagaaggtgg tcttttggag tgggctgagt ttgttggaaa
     6301 ttactatgga acacctttaa aagaagttaa tcgtttacgt gatgaaggaa agaatgtttt
     6361 attagaaatt gaagtgcaag gtgctattca ggtacaggaa aaatgtccgg atgccgtttc
     6421 aatctttatt attccacctt caatggagga attagaaaga cgtattcgtg gtcgtagtac
     6481 agaaccggaa gaaatcattc agcaaagact tgcgaaagct agcaatgaaa tgaagatggt
     6541 atctcaatat aaatatattg tatgtaatga tgatccaaaa cttgcggctg aattgattaa
     6601 gacgattatt cttcgccatg tcgagcttga agaaaacaaa tagttgcaaa taaagtcaat
     6661 ttgtgctata aattatgtgt atagaaatta aaggagtggg gaaccactcc ttatgttttg
     6721 ctactaagga gaaatgtatg gacaatctta aagaatggat tgaagaagtt cttaatgaaa
     6781 atggatgtca tttatatgat cttgaatgga tcacaaatca gggcacacca ttattaagag
     6841 tttcggttga aaaagaagac ggtacaatgg atttggatac ttgtgcatct tgctcagatg
     6901 taattggaca aatgcttgac gaaaaagact ggtattctca tgaatacatg cttgaagttt
     6961 gttcgccagg agcagaaaga gaacttagaa cagatgaaga agttcaaaga gctgttggca
     7021 aatatgtatt tgtgaagatg aaagatccaa aacaaggttt agatcaagtt attggtgatc
     7081 ttgttg
//
LOCUS       DBMDWL010000110         7023 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4051_length_7023_cov_2.605027, whole genome shotgun sequence.
ACCESSION   DBMDWL010000110 DBMDWL010000000
VERSION     DBMDWL010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4051_length_7023_cov_2.605027"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="ACZPDN_07980"
     CDS             <1..1133
                     /locus_tag="ACZPDN_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIR1298601.1"
                     /translation="EFERISYVFYDREVELINAINAHLKTSLYRFKYGIQLGNPMLNN
                     IKTEYEELFELTKRACKILENDLDSLISDAEVAYLTLHFGAFMPTQKANKHAFRIMII
                     CPNGIGTGNMLRQEVSTLVPQATEIKNIPLSSYRPNHDYDVVISTVMLANEQRLIMVH
                     PILTDQDRVAILRNCMATEPQAKMQIDDILKIASNYINPDQLNDFHKNLQDYYSSIQV
                     QKVPHKTYDRGLLDFLKGSHIQCVNSQVSWEEAIHISAQPLLDDGSISNDYVNAIIND
                     QKNKGLYMFLADDLVLAHSAIENGVNRLDVSMCTFKEPVPFLNGQNARIILVLCAEDK
                     TKHIHVLNDMLNIFSKKKSIDQIASLNSPAEIYSYIDKHIEK"
     gene            1143..1799
                     /gene="deoC"
                     /locus_tag="ACZPDN_07985"
     CDS             1143..1799
                     /gene="deoC"
                     /locus_tag="ACZPDN_07985"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1298602.1"
                     /translation="MEFNKYFDHTFLKAFATEKDIDTLCQQARELNTASVCVNEEWVA
                     YCKELLKDTNVMVCSVVGFPLGQCGLNTKAYETKEALKDGADEIDYVLNVGDVKMGKF
                     DKVKEEMETLTNICHEQNKGIKVIFENCYLTKEEIKKCAEIAKEVKPDFIKTSTGFGT
                     GGATIEDVKLMKDTVGSDVEVKAAGGIHSYAEVKEMIEAGATRIGASATVQIVEEYNK
                     "
     gene            1888..3222
                     /locus_tag="ACZPDN_07990"
     CDS             1888..3222
                     /locus_tag="ACZPDN_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298603.1"
                     /translation="MKKNQSVDLLHGPIFKSLSLLAIPIMATYFIQMAYNLVDMLWIG
                     FLGSGAVASVGVSGNFMYLANGLVNMPKIGSQALVGQSLGANNKEETKNYIEAAFQSS
                     ILFALIYGIIIIVFQKNLIQLFNLKDSTIIQDAQNYLWITCGFIIFSFVNQIFTGILT
                     AAGHSKNTFIATTTGLVLNLILDPVLIFVCNLRVIGAALATIIAQIIVTLVFLYDAKK
                     LDLFQEIKLLSKPDKNHISKILKIGFPPSIQSMLFTCISMYIARLISSFGAISVAVQK
                     VGSQIESISWMAADGFSASINAFTSQNYGAKNYNRVQKGYKTGMLVVGLWGLLTSCIL
                     IFLPGPIFSCFIHESDILPYGIDYLVILGFSQLFMCIEIATQGAFNGLGKTLPPSIVS
                     IVFTSARIPMAIVLSHFLGVNGVWWAISISSIIKGTILVTWFILYSKRRLIA"
     gene            3219..4556
                     /locus_tag="ACZPDN_07995"
     CDS             3219..4556
                     /locus_tag="ACZPDN_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298604.1"
                     /translation="MKNHSLTQGSIAKGLLFFVIPLFFSNLFQQLYNTIDTILVGHFL
                     GDNALAAMGATAAIFELIVQFSNGCGTGFSIVTARYFGSRNENELKKSVAASLILGLV
                     ISIVITLISYFSMPYLLTILKTPQSIYHDSLNYIRCISAFLIITMFYNLGAGMLRAIG
                     DSLRPLIVLFCSSIINIALDILCITILDMGVFGAAVATVIAQGISTIICFFLIWRSAK
                     ILIPKKEHFHIESNMVNDLLGQGFSMGFMFSIVSIGTIILQTGINSLGTQIIAAHTAA
                     RKLMSLFCLPLSTLAASMATFVSQNKGAQNYDRIIKGVHLANMCGIIYPIFLSILIFF
                     SADNLVHFLSGSNNPIVLENGAMYLKINIPFFIILSILLNLRNAIQGFGYKITPLISS
                     VIEFLGKIVFTLFFVPIFKYLGVCLCEPIIWCLMASQLLYSYQKIIKPFKAQN"
     gene            complement(4577..5233)
                     /locus_tag="ACZPDN_08000"
     CDS             complement(4577..5233)
                     /locus_tag="ACZPDN_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1298605.1"
                     /translation="MGSNYLIKFLEEKEVPTITKKRHTYLTYYGLEQQDTYVLKEGVI
                     KTSIILRDGREFNISYIKGPDIISLLKDEVSQYTSAPFNVRIESETATFYRIPRVLFW
                     EYVNQDSKLQDYVNSYYRNKLAEAIFRQQLMTMNGKNRAVCAFIYQLIPLFGREVNNG
                     ILIDFQVTNDDIAGFCGISTRNSVNRILRGLREENVITMVNQKIVVLDKDYLKQFIQA
                     "
     gene            complement(5363..5536)
                     /locus_tag="ACZPDN_08005"
     CDS             complement(5363..5536)
                     /locus_tag="ACZPDN_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298606.1"
                     /translation="MKKLGYLAGASLLFVPMPLYAAGIGSSNQIFTHTGWFSVICMAT
                     IGVGVAICSKHQK"
     gene            complement(5652..6083)
                     /locus_tag="ACZPDN_08010"
     CDS             complement(5652..6083)
                     /locus_tag="ACZPDN_08010"
                     /inference="COORDINATES: protein motif:HMM:NF023116.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HIR1298607.1"
                     /translation="MKVSVELNVSDKEFYDVMMKSLQEELNHVSKKKLELKEGLKYKK
                     KSAQRKGIGSEITVYIKKLVPNKLYVATFNTAIDHTMISYKIEALDESKIRVTYEEVY
                     ENISSQNIPAWKQKMAEKQSSKKAKKMFKEIEKFILNERNK"
     gene            6137..6886
                     /locus_tag="ACZPDN_08015"
     CDS             6137..6886
                     /locus_tag="ACZPDN_08015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1298608.1"
                     /translation="MYTDSHCHITCDRLYSRIDEIIENIKTKNITSCMIMCTSPEELE
                     RAKRIKEKYPFFKVAFGWFPSDAKDVTDKEIQYLIDQTPYLDCLGEIGLDYYWDTSFN
                     DLQKELFIKQIQIANEHNLPISIHMRESTKDCMDILKQYAKTKIIFHCFSGSKETMME
                     CLKMNSMISFAGPITFKNARHAPECIKACPIDRIITETDSPYLTPVPFRGKENEPMYV
                     EYVAKKICEIKQLDEANACKQIEENFNSIFR"
BASE COUNT         2333 a         1158 c         1091 g         2441 t
ORIGIN      
        1 atgaatttga aagaatctct tatgtattct atgatcgcga agtagaattg atcaatgcaa
       61 tcaatgctca tttaaaaact tctctatatc gttttaaata tggtatacag cttggaaatc
      121 ctatgttaaa taacataaaa actgagtatg aagaattatt tgaacttaca aaacgtgctt
      181 gtaagatctt agaaaatgat ttagattctt tgatttctga cgctgaagtt gcttatctaa
      241 cattacattt tggggctttt atgcctacac aaaaagcgaa taaacatgca tttagaatca
      301 tgattatctg cccaaatggc attggaacag gaaatatgct acgccaagaa gtttctactt
      361 tagtgccaca agcaaccgaa atcaaaaaca taccactcag tagttatagg ccaaatcatg
      421 attatgatgt tgtaatttca acagtaatgt tagccaatga acaaagatta atcatggttc
      481 atccaatact tacagatcaa gatcgtgtgg caatacttag aaattgtatg gctacagagc
      541 cacaggcaaa aatgcaaatt gatgacattt tgaaaattgc atcaaattat attaatcccg
      601 atcaattaaa tgacttccat aaaaatctac aagattatta ttcaagtata caagtacaaa
      661 aagtacctca taaaacttat gatcgaggtt tattagactt tttgaaagga tcccatattc
      721 aatgtgtaaa ttcacaagtc agctgggaag aagctattca catctcagca caaccattat
      781 tagatgatgg ttctatttca aatgattatg tgaatgccat cataaatgat caaaaaaata
      841 aaggcttata tatgttttta gctgatgatc ttgttctagc acactctgcg attgaaaatg
      901 gcgtaaatcg attagatgta tcaatgtgta catttaaaga gcccgttcct tttttaaatg
      961 gtcaaaacgc cagaattatt cttgtacttt gtgcagaaga taaaacgaaa catattcatg
     1021 tattaaatga tatgcttaat attttctcta agaaaaaaag tattgatcaa atcgcaagct
     1081 taaattcacc agcagaaata tattcatata tagataaaca tatcgaaaaa taggaggtta
     1141 tcatggaatt caataaatat ttcgatcaca catttttaaa ggcatttgcc acagaaaaag
     1201 acattgatac actttgtcaa caagcaagag aattaaatac cgcaagcgtt tgtgttaatg
     1261 aagaatgggt tgcttattgt aaggaattac taaaagatac aaacgtaatg gtttgcagtg
     1321 ttgtgggttt tccactagga caatgtggat taaacacaaa agcttacgaa acgaaagagg
     1381 cattaaaaga tggtgcagat gaaattgact atgtcttaaa tgttggcgat gtgaaaatgg
     1441 gcaaatttga caaagtaaaa gaggagatgg aaactctaac aaatatttgt cacgaacaaa
     1501 acaaaggaat taaagtgatt tttgaaaatt gctatctaac aaaggaagaa atcaaaaaat
     1561 gtgctgaaat tgctaaagaa gttaaaccgg atttcattaa aacaagcact ggatttggta
     1621 ctggcggtgc cacaatagaa gatgtgaaat tgatgaaaga taccgttgga agtgatgttg
     1681 aagtaaaagc agccggtggc atccattctt acgctgaagt aaaagaaatg atcgaagctg
     1741 gtgccactcg tattggtgca agtgcaacag tacaaattgt agaagaatac aataaataat
     1801 tatttgttat gtcaaaaaga aatttaagca tctcttagat ttcttttttt tgttttataa
     1861 ttaaaaaatt gctacaatag tcactgtatg aaaaaaaatc agagtgttga cctattacat
     1921 ggtcctatat ttaaatcgtt aagtttactg gcaattccaa tcatggccac ttattttatt
     1981 caaatggcct ataacctagt tgatatgcta tggattggat ttttgggttc tggcgccgtt
     2041 gccagtgttg gtgtctctgg aaatttcatg tatttggcaa atggattagt aaatatgcct
     2101 aaaattggtt ctcaagcttt agttggacaa agcttaggag ctaataataa agaggaaaca
     2161 aagaattata ttgaagccgc ttttcaaagc tcaatattgt ttgcgttgat ttatggaatc
     2221 attattattg tttttcaaaa aaatttaata caactcttca atttgaaaga ttcaacaata
     2281 attcaagacg cacaaaatta tctatggatt acatgtggtt ttattatttt ctcatttgtg
     2341 aatcaaatct tcacgggtat tttaacggct gccggtcatt caaaaaacac ttttattgca
     2401 accacaacag gtttagtttt aaatcttatt ttagatcctg ttttgatttt tgtttgtaac
     2461 cttagagtca ttggtgccgc tctcgcaacc attattgccc aaattattgt tactttagtt
     2521 tttttatatg atgctaaaaa attagattta tttcaagaaa taaagttatt atcaaaacca
     2581 gataaaaatc acatatctaa aattttaaaa ataggcttcc caccttctat tcagagcatg
     2641 ttatttacat gcatctccat gtacattgct cgtttaattt ctagctttgg agctataagt
     2701 gttgccgttc aaaaagtagg atctcaaatc gaatccattt catggatggc tgccgatgga
     2761 tttagtgcat cgatcaatgc gttcacttcg caaaattatg gagcaaaaaa ctataatcga
     2821 gttcaaaaag gttataaaac tggaatgctt gtcgttggtt tatggggatt gcttacttca
     2881 tgtatattaa ttttccttcc tggtcctatt ttttcttgtt ttatccacga atccgatatc
     2941 cttccatatg gaattgacta tctagttata cttggatttt cccaactctt catgtgcata
     3001 gaaatcgcga cacaaggagc gtttaacgga ttaggtaaaa cattacctcc aagtattgtt
     3061 tctattgttt ttacttctgc cagaattccc atggccattg tattatctca ttttttaggt
     3121 gttaacggcg tatggtgggc tatctctatt tctagtatta taaagggtac tattttagta
     3181 acatggttta ttttgtattc aaaaaggagg cttatcgcat gaaaaatcac tctttaaccc
     3241 aaggaagcat cgcaaaagga ttacttttct ttgtgattcc acttttcttt tccaatctat
     3301 ttcaacaact ctataacaca attgacacta tattagttgg tcatttttta ggggataatg
     3361 cactggctgc tatgggagca acagctgcaa tctttgaatt gattgtacag ttttccaatg
     3421 gctgtggaac tggttttagt attgttacag ctcgttattt tgggtcaaga aatgaaaatg
     3481 aattaaaaaa aagcgtcgcg gcttctttaa ttttaggact cgttatttca atcgtcatta
     3541 ctcttatttc atatttttcg atgccctacc tattaacaat tctaaaaacc ccacaaagta
     3601 tttatcatga ttcattaaac tacatccgct gtatttcagc tttcttgatt attactatgt
     3661 tttacaattt aggagccggc atgttacgag ctataggaga tagtctacgt cctttgattg
     3721 tgcttttttg ttcttctatc atcaatattg cacttgatat actttgtatc acaatacttg
     3781 atatgggtgt cttcggagct gctgttgcga ctgttattgc acaaggcata tctaccatta
     3841 tatgtttctt tctaatttgg agaagtgcta aaatattgat tccaaaaaaa gaacactttc
     3901 atattgagtc gaatatggtt aatgatttat tgggacaagg attttcaatg ggatttatgt
     3961 tttccattgt atccatcggt acaatcattc ttcaaacagg aatcaatagt cttggaacac
     4021 agattatagc ggctcataca gctgcacgaa agttgatgtc tttattttgt cttccacttt
     4081 ctactcttgc cgcttctatg gcaacctttg tatctcagaa taaaggtgca caaaactatg
     4141 atcgtattat aaaaggtgtc catcttgcga atatgtgtgg catcatttac ccaatattct
     4201 taagtattct tatctttttt tcagcagata acttagttca ttttctttct ggatccaaca
     4261 atcctatagt acttgaaaat ggagctatgt atttaaaaat caacattcca ttcttcataa
     4321 ttctaagtat cttacttaat ttaagaaatg cgattcaagg ttttggatat aaaatcacgc
     4381 cattaatttc gagtgtaatt gagttccttg gaaaaatcgt ttttactcta tttttcgttc
     4441 caatatttaa atatttaggt gtttgtctat gcgaacccat tatctggtgc ttaatggcca
     4501 gccaattatt atattcatat caaaagatta taaaaccatt taaagcgcag aattaacttc
     4561 tgcgccttgt tttgtattat gcctgaataa actgttttaa ataatctttg tctaaaacga
     4621 ctattttttg attcaccatt gtgatgacat tttcttcacg taaacctctt aaaatacgat
     4681 ttacactatt tcttgttgaa ataccacaga atcctgcaat atcatcattt gtaacttgga
     4741 aatcaatcaa aataccatta tttacttccc ttccaaatag cggaatcaat tgataaataa
     4801 atgcgcaaac agccctattc ttgccgttca ttgtcataag ttgctgtcta aaaatagctt
     4861 ctgctaattt attacgataa tacgagttta cataatcttg taattttgaa tcttggttta
     4921 catattccca aaataacact cttggaatac gataaaatgt cgctgtttca ctttctatac
     4981 gaacattaaa tggtgccgat gtatattgac ttacttcatc ttttaaaagc gagataatat
     5041 ccggtccttt aatataagat atattaaatt cgcggccatc acgcaaaatt atacttgtct
     5101 taataacgcc ttcttttaaa acatatgtat cttgttgttc caatccataa tacgttaaat
     5161 atgtatgtct tttctttgtg attgtaggca cttctttttc ttcaagaaat tttattaaat
     5221 aattactgcc cataaaatat ccctttctgt atcagttaaa tcagtataac acaatcccta
     5281 aaacaagtag gtaacatatg ttattcataa ttctttaata caaaagaaaa agggatacaa
     5341 ctattgtatt ccctcttcac tattattttt gatgttttga acaaatcgct actcctacac
     5401 caattgttgc catacaaatt actgagaacc atccagtgtg agtaaaaatt tgattgctag
     5461 aaccaattcc tgctgcatac agaggcattg gaacaaacaa taatgatgct cctgctaaat
     5521 accctaactt tttcatatcc aatccctcct tttagttggg ttaaagcttt tctacacaaa
     5581 ctttataacg cattcataga aaaaaagaaa ccaatatggt aaacttggcc tcttttatgg
     5641 aataatctgc tttatttgtt tctttcattt aaaatgaatt tttcgatttc tttaaacatc
     5701 ttcttagctt tcttgctaga ttgcttttcc gccatttttt gtttccaggc aggaatattt
     5761 tgagaagata tattttcata cacttcttca taagtgactc taatttttga ttcatctaaa
     5821 gcctcaatct tatatgaaat cattgtatga tcaatagccg tattaaaggt tgctacatat
     5881 aatttatttg gaactaattt ttttatatag acagtaattt cggatccaat cccttttctt
     5941 tgtgctgatt ttttcttata tttcaatccc tcttttaatt ccaatttttt ctttgaaaca
     6001 tgatttaatt cttcctgcaa tgatttcatc atcacatcgt aaaactcttt atcactcaca
     6061 tttaactcta cactaacttt catgttgcac ctcgcttaac acaattatat actattttaa
     6121 agaaagaagg gtatttatgt atacagatag tcattgtcac atcacttgtg atcgtttata
     6181 tagtcgaatt gacgagatta ttgagaatat taaaactaaa aatattacat cttgcatgat
     6241 tatgtgcaca agtccagaag agttagaacg agcaaaaaga ataaaagaaa aatatccatt
     6301 ctttaaagtg gcgtttggat ggtttccaag cgatgctaaa gatgttacag ataaagaaat
     6361 tcagtatctc atcgatcaaa cgccttattt agattgttta ggagaaattg gattggatta
     6421 ttattgggat acaagtttta atgatttaca aaaagaactg tttatcaagc agattcaaat
     6481 cgcaaatgaa cacaatcttc ccatttcgat tcatatgcgt gaatccacaa aagactgcat
     6541 ggacatttta aaacaatacg caaaaacaaa aatcatcttc cattgtttta gcggaagtaa
     6601 agaaacaatg atggaatgtt taaaaatgaa ttctatgatt tcttttgcag gacctatcac
     6661 ttttaagaat gctagacatg cacctgaatg cattaaagca tgcccaattg atagaattat
     6721 tacagaaaca gattctccat acctaacacc cgttccattt agaggcaaag aaaatgagcc
     6781 tatgtatgta gaatatgtcg caaaaaagat ttgtgaaatt aaacaacttg atgaagccaa
     6841 tgcatgtaaa caaatagaag aaaatttcaa ttccattttt agataatgaa agacctgtca
     6901 aaatttgaca ggtctatttt tgtaacttct cgtattctga aactttgttt cctatgataa
     6961 tcatatcgcc ttgaatcaca ataggtcttt ttactaacat accattggaa gcaagcaaat
     7021 caa
//
LOCUS       DBMDWL010000111         6974 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4089_length_6974_cov_2.315583, whole genome shotgun sequence.
ACCESSION   DBMDWL010000111 DBMDWL010000000
VERSION     DBMDWL010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_6974_cov_2.315583"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="ACZPDN_08020"
     CDS             <1..978
                     /locus_tag="ACZPDN_08020"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR1298609.1"
                     /translation="AQMLMPYHQYQDELEEQAASNKIGTTKRGIGPCYADKYARHGIR
                     YHEFKDFEHFKVRLKDCLDFKNKLFTAVYGGKAMDYDKMVADFEAIYDRIVPMIQETT
                     HLVNEALDEDKTVLFEGAQAAMLDINYGTYPYVTSSSPTSAGVTTGACIAPSRIQTVV
                     GVVKAYSTRVGEGPFVTELLDEQGEWIREHGGEYGATTGRPRRCGWLDLLTVKHANLM
                     SGLTDIVITKIDVLDGLDEIKVCVGYEIDGKEYNYIPSDQADVAKAKPIYKTFKGWKT
                     DITKMTSVDQLPQECLDYVHFIEEFTGVRVSMISVGPDRVNNIYVHEIK"
     gene            1020..1850
                     /locus_tag="ACZPDN_08025"
     CDS             1020..1850
                     /locus_tag="ACZPDN_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1298610.1"
                     /translation="MILDTIHGHYNSFFDSEKKIADYIIQHPKEVVNMTIKELAQACN
                     TSQASVSRFARKCQMDSFHHLKVNLARDCVNEIELKSNTISTSDISGSLETILSNKIS
                     ELTATIRNIDVDQLSVILSLIQNAKHVLVCAVGNTIPVALDCMFKFNEIGILTFTLTI
                     WENQASYALGLDANDVVIAISNSGESSQVLAVCREMTKRGVPTIGITNNEDSVVAKEC
                     LYHIQTSTREKLFLNEFCFSRIPVATVIEILYLFLTNERTDSYNRISQCEELYASLKV
                     "
     gene            1937..2611
                     /gene="rpiA"
                     /locus_tag="ACZPDN_08030"
     CDS             1937..2611
                     /gene="rpiA"
                     /locus_tag="ACZPDN_08030"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HIR1298611.1"
                     /translation="MKKKCALYASKLVKDGMVVGLGGGSTIQYLIDYVKDKDIQVVTP
                     SAKTALKARKEGLTVLNTQYVDHVDIAFDGCDEVDCNLNALKSGGGIHTQEKIIASMA
                     DEYIVLVDETKFHKTLAFQVPVVVEVLPKAYSIVKKKLEELNGIVEWRTASNKDGFLI
                     SDEGNILLDVYFKNVEDIRLLNQTLLMMPGIVDTSLFVDILTGMILVNDMGVFKKEKD
                     GEFHSL"
     gene            2649..>2888
                     /locus_tag="ACZPDN_08035"
     CDS             2649..>2888
                     /locus_tag="ACZPDN_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR1298612.1"
                     /translation="MANALIQEDVGVYGEASGHYSSTKKFSEEFSIKHVYENYQAIFK
                     DGNIDIVYNATPHNSHYEIMKDALLHNKHVFSEKAI"
     assembly_gap    2889..2898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2899..3203
                     /locus_tag="ACZPDN_08040"
     CDS             <2899..3203
                     /locus_tag="ACZPDN_08040"
                     /inference="COORDINATES: protein motif:HMM:NF046806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR1298613.1"
                     /translation="NHKKRNLVISDGVTLFHMPLFKELQKKVQLNDLGPVKMIQVNFG
                     SFKEYDVKNRFFNPDLAGGALLDIGVYAVSFARWFMANKPNTVLTAMTPFETGVDE"
     gene            3196..3504
                     /locus_tag="ACZPDN_08045"
     CDS             3196..3504
                     /locus_tag="ACZPDN_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298614.1"
                     /translation="MNKNIENEIATISLTMCAKQPKRGLVACENGYIEIYNFPRADKA
                     VITYTSDGHTDQITCGESCLALNYKIRDMEEYVTDLSGKQNPRCDGCVNSSTESVGEI
                     "
     gene            3501..4274
                     /locus_tag="ACZPDN_08050"
     CDS             3501..4274
                     /locus_tag="ACZPDN_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR1298615.1"
                     /translation="MNKKYFFFDIDGTLTDRKTGKIVPSAQVALNELQKAGHFVAIAT
                     GRAHYKARKFMESVGLHDMVCCGGGGLVIHDELVQNIPLDLEKSKAIAKQALSLGYGV
                     LFALDDSIKVYTLDHTFQNQVGNRKEPTEYIYDENLDVDELNVIYKMYISIPKEKEEE
                     LTLKDTLGNMRFVPDYLMFQYDAKHQGILDMMERIGGDLKDVVVFGDDTNDMVMFDPQ
                     WTSVAMGNACQELKDIATLVTDANVDDGIYNICKKMEWI"
     gene            4347..5300
                     /locus_tag="ACZPDN_08055"
     CDS             4347..5300
                     /locus_tag="ACZPDN_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1298616.1"
                     /translation="MPTRKEVLIRSANLLNDFAFKYVFGEDCKEANDALKSLLTVFLE
                     RKVNKVVVKNSEIVKDFSKMKSPRLDLLVEFDDRTTVDLEMQLRQTKDNLPIRFSYYL
                     ARLHGCQELEGKYYGELKETIVLVFFNVNLIDNHRMCNTFTLRNEEGLSFVEESQDRM
                     KLRTVEMAKLDSNKPLKDMNEQEKMIYYFLNCHKGMEDSKIKVMIESDGVIQMLEKRV
                     ETISDDGWKKIIEDFQKLHENEERMERQLELEEAHKQVEEAHKQVEDANKQVEDANKQ
                     VEQKNQEIKQMIQLMLTNGMSLEFISKSLSKSEDEIRDLLR"
     gene            5428..6690
                     /locus_tag="ACZPDN_08060"
     CDS             5428..6690
                     /locus_tag="ACZPDN_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR1298617.1"
                     /translation="MQKIQNVLMKYLMPIANKLEQQKHIQAIKDGIVASIPIIIVGSF
                     CTIFLGIANLLGSGPVYDFLNANMGVLTYASAFTNDMLAVYATYFIAKTLSEKYDLKG
                     SQPAVTALVVFFILSAVVKDGQLNTSYLGSTGLFTGIVAALFTVEIYHLCYERKWYIK
                     MPESVPPMVQDMFAALVPLVLNTIIAVAIANLSLTFGKVAFPQLIMNVLAPAITGMDT
                     LPALLIVMFFTQLLWFFGLHGPSITSAVWASFAIAYAAENIAAYTAGQPVQHIFTFGL
                     FYCILCVSGSGLTLGLNILMMRSKAKSLSSVGKVSIIPGLFGINEPLIFGTPIILNPF
                     MFIPFVFGPLICTTIVYLTMNLGLVGKPIANPPGFLPPGVSAFLMTLDWKAVVLVFVL
                     LILQTVFYYPFFKMMEKEELQKEQALEK"
     gene            6701..>6974
                     /locus_tag="ACZPDN_08065"
     CDS             6701..>6974
                     /locus_tag="ACZPDN_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019884.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIR1298618.1"
                     /translation="MLTARQLKIIKLIMNNPGIHGKEISENLNVSTRTIRNEITFMND
                     VTNCILIRSSTKQGYMINDEHLDIIHNIVNQDNENMDLAVYRIYKII"
BASE COUNT         2389 a          902 c         1441 g         2232 t
ORIGIN      
        1 gcacagatgt taatgccata tcatcaatat caagatgaat tagaagagca agctgcatct
       61 aataaaattg gaactacaaa acgtggaatt ggtccatgtt atgcagataa gtatgctcgt
      121 catggtattc gttatcatga atttaaagat tttgaacatt ttaaagttcg tttaaaagac
      181 tgtttagatt tcaaaaacaa attatttact gccgtatatg gtggaaaagc tatggattac
      241 gataaaatgg tagctgattt tgaagctatt tatgatcgta ttgtccctat gattcaagaa
      301 acaacacact tagtaaacga agctttagat gaagataaga cagtattgtt tgaaggggct
      361 caggcagcta tgcttgatat caactatggt acatatcctt atgtaacttc ttcaagtcca
      421 actagtgctg gtgttactac aggtgcttgt attgcaccaa gtcgtattca gactgttgta
      481 ggtgtagtaa aagcatattc aacacgtgta ggagaaggac cattcgtaac tgaattatta
      541 gatgaacaag gtgaatggat tcgtgaacac ggtggagaat atggagctac tacaggtcgt
      601 cctcgtcgtt gtggatggtt ggatctatta acagtaaaac acgcaaactt aatgagtgga
      661 ttaactgata tcgttatcac taagattgac gtattggatg gtttggatga aatcaaggtt
      721 tgtgttggat acgaaatcga tggtaaagaa tataactata ttcctagtga tcaggcagat
      781 gttgccaagg ctaaaccaat ctataaaact ttcaaaggtt ggaagacaga tatcactaag
      841 atgacttcag tcgatcaatt acctcaagaa tgtttagact atgtacactt cattgaagag
      901 ttcactggcg tacgtgtttc tatgatttct gtaggaccag atcgtgttaa caatatttac
      961 gtacatgaga tcaagtaatt tgatctcttt tttgataata tagaggtggt ggtgatttta
     1021 tgatactcga tacaattcat ggacattata attctttctt tgattctgaa aagaagattg
     1081 cggattatat tatccaacat cctaaagaag tggtgaatat gacgattaag gagcttgccc
     1141 aagcatgtaa tacttcgcag gcgagtgttt cgcgttttgc cagaaaatgt cagatggata
     1201 gttttcatca tttaaaggtg aatttggcta gagattgtgt gaacgaaatt gaactaaaat
     1261 caaataccat ttctacaagt gatatttcag gttctttaga aaccattctt tccaataaga
     1321 tttcagaact tacggctaca atacgtaata tagatgtgga tcaattgagt gtgattcttt
     1381 ctttgattca aaatgctaaa cacgtacttg tgtgtgctgt aggaaatacg attccggtag
     1441 cgcttgattg tatgtttaaa tttaacgaga ttggtatttt aacctttaca ttaaccattt
     1501 gggaaaatca ggcatcttat gcattaggac tagatgcaaa tgatgtagtg atcgccatct
     1561 caaatagtgg agagagcagc caggttttag cagtttgtag ggaaatgaca aaacgtgggg
     1621 taccaacaat aggaatcaca aataatgagg attcagtggt tgccaaagaa tgtttgtatc
     1681 atattcagac atctacgcgt gaaaagctat ttttaaatga attttgtttt tcaagaattc
     1741 ccgttgcgac cgtcattgaa attttatatt tatttttaac gaatgaacgc acagatagtt
     1801 ataatcgaat cagtcaatgt gaagaattat atgcttcttt gaaggtatag ttcttctttt
     1861 tttgcgtgaa tgataattac attaaaagta tattgacgaa aataaattac atgatatatt
     1921 gaaggtggtg atgattatga aaaagaaatg tgcattgtat gcatcaaaat tagtgaagga
     1981 tggaatggtt gtaggcctag gcggtggcag taccattcaa tatttgattg attatgtaaa
     2041 ggataaagat attcaagtgg ttacaccttc tgcaaaaaca gctttaaaag cccgaaaaga
     2101 aggattaact gtattgaata cacaatatgt ggatcatgtt gatattgcct ttgatggatg
     2161 tgatgaagta gattgtaatt taaatgcttt aaagagtggt ggaggtattc atacgcaaga
     2221 aaaaattatt gcgtctatgg ccgatgaata tattgtattg gttgatgaaa ctaaatttca
     2281 caagactttg gcatttcaag tgcccgttgt tgtggaagta ttacctaaag catatagtat
     2341 tgtaaaaaag aaattagaag aattaaatgg gattgttgaa tggcgtacag cttcaaataa
     2401 agatggcttt cttattagtg atgaagggaa tatcttatta gatgtgtact tcaaaaatgt
     2461 tgaagatatt cgtttgttga atcaaacctt gttgatgatg ccaggtattg tggatacaag
     2521 tttatttgtg gatatattga caggtatgat tctagtgaat gatatgggtg tattcaaaaa
     2581 ggaaaaggac ggagaatttc attcattata attgggaaat tgttggtcct ggctggattg
     2641 cgcatgatat ggcaaatgct ttgattcaag aagatgttgg cgtttatggt gaggcttctg
     2701 gtcattatag ttccactaaa aagttttctg aagaattttc aattaaacat gtgtatgaaa
     2761 attatcaagc tatatttaaa gatggaaata ttgatattgt ctacaatgca actccacata
     2821 attcgcatta tgaaattatg aaggatgctt tgcttcataa taaacatgtt tttagtgaga
     2881 aggcaatcnn nnnnnnnnca aatcacaaaa aaagaaattt agtcatcagt gatggtgtca
     2941 ctttatttca tatgcctttg tttaaagaac ttcaaaagaa ggtacaattg aatgatttgg
     3001 gtcccgtaaa aatgattcaa gtaaattttg ggagctttaa agaatatgat gtaaagaatc
     3061 gcttctttaa tccagatcta gcaggcggtg ccctattaga tattggtgtc tatgccgtaa
     3121 gttttgcgcg ttggtttatg gcaaacaagc caaatacagt attaactgcg atgacgccat
     3181 ttgaaacggg tgtggatgaa taaaaatata gaaaatgaaa ttgcgacaat ttctttgaca
     3241 atgtgtgcta aacagccaaa gcgtgggtta gtggcttgcg aaaatggata tattgaaata
     3301 tataattttc ctagagcgga taaggctgta ataacatata cgagtgatgg acatacagat
     3361 cagattactt gtggagaaag ttgtttggca ctaaattata aaattcgaga tatggaagaa
     3421 tatgttacgg atttgagtgg gaaacaaaat ccaagatgtg atggatgtgt taactcaagt
     3481 acagaaagtg taggggaaat atgaataaaa aatacttttt ctttgacatt gatggcactt
     3541 taacggatcg caaaactggt aagatcgtac caagtgcaca agtggcttta aatgaacttc
     3601 aaaaggcagg tcactttgta gctattgcga caggtcgtgc tcactataaa gcacgtaagt
     3661 ttatggaaag tgtaggtctt catgatatgg tttgttgcgg tggtggcgga ttagtcattc
     3721 atgatgaatt ggttcaaaat attcctttag atttagaaaa atcaaaggca attgccaaac
     3781 aagcattatc tttaggatat ggtgttttat ttgcgttaga tgattcaatc aaagtatata
     3841 cattagatca tacgtttcaa aatcaagtag gaaatcgtaa ggaacctaca gaatatattt
     3901 acgatgagaa cttggatgta gacgaattga atgtgattta taaaatgtat atttctattc
     3961 caaaagaaaa agaagaagaa ttaacattaa aggatacatt aggaaatatg cgttttgtac
     4021 cagattattt aatgtttcaa tatgatgcaa aacatcaagg catcttggat atgatggaac
     4081 gtattggtgg tgatttaaaa gatgtagttg tctttggcga tgacacaaat gatatggtta
     4141 tgtttgatcc gcaatggaca agtgtagcga tgggaaatgc gtgccaggaa ttaaaggata
     4201 tcgcaactct agtcacagat gccaatgtag atgatggaat ttataatatt tgtaagaaaa
     4261 tggaatggat ttaaggcact aaaaagacat ttttttgatt tttattcgaa aaagtgtctt
     4321 tttttacgta tatgagtatg taggaaatgc ctactagaaa agaggtgctt ataagaagtg
     4381 caaatttatt aaatgatttc gcctttaagt atgtatttgg cgaagattgt aaggaggcca
     4441 atgatgcctt aaaatcatta ctcacggtat ttttagaaag aaaggtgaat aaggttgttg
     4501 taaagaattc agaaatagta aaagacttta gcaaaatgaa aagtccgcgc ttagacttat
     4561 tagttgaatt tgatgatcgg acaacggtag atttagaaat gcagttaaga caaacgaaag
     4621 ataatttgcc aattcgcttt agttattatt tggctagact tcatggttgc caagaattgg
     4681 aaggaaaata ttatggagag ttaaaggaaa cgattgtctt agtgttcttt aatgtgaatt
     4741 taattgataa tcatagaatg tgtaacacat tcacattaag gaatgaagaa ggtttatctt
     4801 ttgtagaaga aagccaggat cgtatgaagt tacgtacagt tgaaatggca aagttagatt
     4861 caaataaacc gcttaaagac atgaatgaac aggaaaagat gatttattac tttttgaatt
     4921 gccacaaggg aatggaagat agtaaaatta aggtgatgat agaaagtgat ggggtgattc
     4981 aaatgttaga gaagcgtgta gaaaccatat cggatgatgg ttggaagaaa ataatagaag
     5041 attttcaaaa attgcatgaa aatgaggaac gaatggagcg acagcttgaa ctagaagaag
     5101 ctcataagca agtagaagaa gctcataaac aagtagaaga tgcgaataaa caagtagaag
     5161 atgcgaataa acaagttgag cagaagaatc aagaaataaa acagatgatt caattgatgc
     5221 ttacgaatgg aatgagttta gaatttattt ctaagtcgtt gtctaagagt gaagatgaaa
     5281 ttagggactt attgagatag ccacttttta gtggcttttt tttatttccg ctttaagtgg
     5341 aaaaagaaaa tttgagaatt atagcgagaa catttaatat atagataaca atttggtcga
     5401 caaaaattgt atacgaggag agcgaaaatg caaaaaattc aaaatgttct tatgaagtat
     5461 ttgatgccaa ttgcgaacaa gcttgaacaa caaaaacaca ttcaagcaat caaagatggt
     5521 atcgttgctt caatacctat tattatcgtt ggttcattct gtactatttt cttaggtatt
     5581 gcaaaccttt taggaagtgg acctgtttat gattttttaa atgcgaatat gggtgtcttg
     5641 acatatgcat cagcattcac aaacgatatg cttgcggtat atgcaacata ctttattgcg
     5701 aagactttat ctgaaaaata tgatttaaaa ggaagtcaac ctgctgttac agctttagtt
     5761 gtattcttta ttcttagtgc tgtagttaaa gatggtcaat taaatacatc ttatttagga
     5821 tccactggat tatttactgg tattgttgca gctttattta cagttgaaat ttatcacctt
     5881 tgttacgaac gtaaatggta tattaaaatg ccagaaagtg taccaccaat ggtacaagat
     5941 atgtttgcgg cattagtacc attagtattg aacacaatta tcgcagttgc gattgcgaat
     6001 ctttcattga cttttggaaa ggttgccttc ccacaattga ttatgaatgt attagcacca
     6061 gctataacag gtatggacac actaccagct ttattgattg tgatgttctt tacgcaatta
     6121 ttatggttct ttggattaca tggaccaagt attacatctg ctgtatgggc atcttttgcg
     6181 attgcgtatg ccgctgaaaa tattgcagca tatacagcag gacaacctgt tcaacatatt
     6241 tttacttttg gattattcta ttgtatatta tgtgtatcag gatctggttt aacattagga
     6301 ttaaatattt taatgatgag atcaaaggca aaatcattga gttcagtagg taaagtatct
     6361 attatccctg gcttgtttgg aatcaatgaa ccattaattt ttggtacacc aattatctta
     6421 aatccattta tgtttattcc atttgtgttc ggaccattga tctgtacaac aattgtatat
     6481 ttaacaatga atttaggctt agtcggtaaa ccaattgcca atcctccagg attcttgcct
     6541 ccaggagtaa gtgcattctt aatgacatta gattggaagg ctgtagtcct agtatttgta
     6601 ttgttgattc tacaaactgt gttctattat ccattcttta agatgatgga aaaagaagag
     6661 ctacaaaaag aacaagcttt agaaaaatag gaagagaaac atgttaacag ctagacaatt
     6721 aaaaattata aaattgatta tgaataatcc ggggattcat ggaaaagaaa tatccgaaaa
     6781 tctaaatgtg tcaacacgaa ctatacgtaa tgaaatcaca tttatgaatg atgtgacaaa
     6841 ctgtatatta atacgttcga gtaccaaaca aggatatatg atcaatgatg agcacctaga
     6901 tattattcat aacatagtca atcaggataa tgaaaatatg gatctagctg tctatcgtat
     6961 ttacaaaatt atag
//
LOCUS       DBMDWL010000112         6928 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4135_length_6928_cov_1.955471, whole genome shotgun sequence.
ACCESSION   DBMDWL010000112 DBMDWL010000000
VERSION     DBMDWL010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4135_length_6928_cov_1.955471"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(113..940)
                     /locus_tag="ACZPDN_08070"
     CDS             complement(113..940)
                     /locus_tag="ACZPDN_08070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1298619.1"
                     /translation="MLKIGSCTVLFNPDRDVISNVETYKDLVDVCVVVDNSENKNEVS
                     KYFEEDSSYIYIDMHGNQGIAAALNAGIEYLHSKNMDMALTMDQDSLFPTSDYLAIYQ
                     LIEKYKDEYSLIGLDVNRVDPSKEIVLVPYWITSGNFVNISDFYLAGKMNESLFIDYV
                     DFDLGYRFKKAHLKIGYLSGYCITHTIGNPIPIHVLNKTFYALNHSPIRYYYRYRNAY
                     YLYHYVDKEFFKKEYRREMFGSLIKMFIFEKKRNEKIKMIKKGLSDAKKRRLGKFNV"
     gene            complement(933..1649)
                     /locus_tag="ACZPDN_08075"
     CDS             complement(933..1649)
                     /locus_tag="ACZPDN_08075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1298620.1"
                     /translation="MISVCMATYNGEKYLKDQLDSILKQIQSSDEVIISDDGSNDSTR
                     YIIEKFQKEYKNIYLVDGPKRGVQKNFENALKHARGDILFLSDQDDIWMDGKVERVLK
                     EFENSKTLCVVHDAEIVDGNMNKVKDSFFELKNGKPGLLHNLYRNAFIGCCMAFRKCV
                     FEKSYPFPDHIEMHDWWIGLVAECMGNTVFIYEPYLKYRRHGDNASSLHHHPLKKMIF
                     NRLYFIEQLMIRWIQVGWHA"
     assembly_gap    1845..1854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1855..2188)
                     /locus_tag="ACZPDN_08080"
     CDS             complement(<1855..2188)
                     /locus_tag="ACZPDN_08080"
                     /inference="COORDINATES: protein motif:HMM:NF022521.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 family protein"
                     /protein_id="HIR1298621.1"
                     /translation="MKRLMNASIVYGVLGLIGGVFYREFTTLSVVHTHYLMLEMVLFL
                     LLVVVEKNFHFVDNKVLKYLTFYHIGLNLTVVMLVIRGVVQVFSLDVSSAAISGVAGI
                     GHLILGISM"
     gene            complement(2414..2926)
                     /locus_tag="ACZPDN_08085"
     CDS             complement(2414..2926)
                     /locus_tag="ACZPDN_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298622.1"
                     /translation="MITEMCDSITVNDLSIQKINVKVAELESIKLEIEDAKEEFDSFT
                     ANHPLQSNKIRSGIVKSLYEKIDVMKEAYRNYEEALKAFENADMLNSENVKALELASK
                     NYTLARLDLENANNNLKVYLENVEDDVEIKEKESVNTATETALGFYTLSGILGLAGLA
                     LAGKKMKRKE"
     gene            complement(3021..5615)
                     /locus_tag="ACZPDN_08090"
     CDS             complement(3021..5615)
                     /locus_tag="ACZPDN_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012415.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR1298623.1"
                     /translation="MKNRNKTLTSLAAASMVLTQVGQMSVFAKEEPMKNLSDTEEVSV
                     EKTQKELLEEQIKNILDNVNEAKKKFDEAQEKYETYNKNDYAFAVSNRDLAQKNYLSA
                     KDDAQEAIVSALEKQIQELEANQAALKDANNKKKGLESRLENANEELKQAQDDLIEQQ
                     RKYESLLNGSSKEQIEQSVNELKTQLESATLAYQAASNRVNELLNAKQEAEAKVLDLQ
                     NTLEGARVELSNAKSDVVNAQNAYDVASADYNEKLEIYNGASNPELKAEYEKQVAEAK
                     NTLDLAQGELQNAKLIQQSKVDAVANAESCVVEVENEICNLKSLIDAKENELLGINES
                     IGIVEKELSAAKEQLDRAIESENSMQEALNKAQIALDKAKKEVEAQQTNVDKAQSDVN
                     AQQKIVNQLRVDKEQVSQKISLGSKGFFEAYGYTNALKVLEENSVEIGKYTEVGAEND
                     ATSLENFKKAIAMVKTGNTLRTTDDNFKGLNPLKVSAEMFAISQVQVNQMAKTEYGHT
                     RLYNVSENIAVGYDDPYVGWYTEEKAVYDYLQKKGWDINDIRDSQGNYIDVNKANEIV
                     KACNLPDIPWVQTGHYTNILKSRYVGTGTAWIEGYTSNGHHKHAGQVFSSQCSLDSLL
                     TIDEFESQFNTYYDSLMNADGAYEDANVILNQLKGELYKQNALLTQKTNDQKVTEQNK
                     VNAENKLNSAKNEVGRLESVVRKKQKNLDDLCSEETTSNIVNEIQNLKALKSNKESLD
                     LVVARQEVENAKKEMAEANVNVNNKEALVKNAQMELDKKNEILEESNKGKEIAYNNLK
                     ISETVLKEKGKVLEDTKKVVFEKQSNVTSKESELDCASELLDGISKDLSECEKDGDIK
                     KVEMNIYK"
     gene            complement(5739..>6369)
                     /locus_tag="ACZPDN_08095"
     CDS             complement(5739..>6369)
                     /locus_tag="ACZPDN_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298624.1"
                     /translation="KDYKTAQNEYNEMLRLQEEAYNQIIEDEYITLDGLPTIHVIPGN
                     SLKIGDVQKYMDLFVKSQPNFLLSNCRMIHICEPKNFIDIAIADGVDVTDDGLGTAYA
                     YASSDDFSITLQIDVDEDYGQKDAVSHELSHIFDFASGSGYGDYGISNSAQLQSLYQN
                     YPDCVGAYGATDSAEYFAQAGAMYVNDPENLKSVCMDLYNFVDSLYHMY"
     assembly_gap    6370..6379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6380..6827)
                     /locus_tag="ACZPDN_08100"
     CDS             complement(<6380..6827)
                     /locus_tag="ACZPDN_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298625.1"
                     /translation="MNRSKVMFSGLVFSVVFGLMYWYRDLLGNKEITIMDQSLINYFD
                     LKLCLTVAVLSMLLIVVLLYSKEVDPDQYRFEYIRSTLSEDELKRIDGLDKEGRRIAY
                     EKRSNEFSYKQILECRNYVNENKPKTSWLLKVGLLSLISAALVMVLS"
BASE COUNT         2258 a         1539 c          675 g         2436 t
ORIGIN      
        1 aaactactta ttttatagtc aaaatgactg aataaccaat acatctcatt tcctgcagga
       61 attattccca cgcacaaaag tataccataa ttacaacact aaaacaaaaa aattagacat
      121 tgaactttcc taaacgcctt ttcttcgcgt ctgaaagtcc ctttttaatc atttttatct
      181 tttcatttcg cttcttttca aaaataaaca tcttaatcaa actaccaaac atctcgcgac
      241 gatactcttt tttaaagaat tctttatcca cataatgata taaataatac gcattccgat
      301 accggtaata ataacgaatt ggcgaatgat tcaacgcata aaaagtctta ttcaacacat
      361 gaattggaat gggatttcca attgtatgcg ttatacaata tccacttaaa taaccaatct
      421 ttaaatgtgc ctttttaaat ctatatccca aatcaaaatc cacatagtca ataaataaag
      481 actcattcat tttaccagcc aaatagaaat ctgaaatatt cacaaaatta cccgaagtaa
      541 tccaataagg aactaaaaca atttccttac ttggatccac tcgattcaca tccaaaccaa
      601 ttaatgaata ttcatcctta tatttttcaa tcaattgata aatagctaaa taatccgatg
      661 taggaaataa actatcctga tccattgtca aagccatatc catattttta gaatgaagat
      721 attcaatacc agcatttaat gcagccgcaa tgccctgatt gccatgcata tcaatgtaaa
      781 tatatgaaga atcttcctca aaatatttcg acacttcatt tttattttcc gaattatcca
      841 ctacaacaca aacatccact aaatctttgt atgtttcaac attcgaaatc acatccctat
      901 ccggattgaa taatactgta caagaaccta ttttaagcat gccatcccac ctgtatccaa
      961 cgaatcatca attgttcaat aaaatataag cgattaaaga tcattttctt taatggatga
     1021 tgatgcaaag aagacgcatt atcgccatgc cttctatatt ttaaatacgg ctcgtaaata
     1081 aataccgtat tccccataca ttcagccact aaaccaatcc accaatcatg catctcaata
     1141 tgatctggaa acggataact cttctcaaaa acacatttac gaaatgccat acaacatcca
     1201 ataaacgcat ttctatataa attatgtaaa aggccaggct tcccattctt caattcaaaa
     1261 aaggaatcct tcaccttatt catatttcca tccacaattt cggcatcatg aaccacacac
     1321 aaagtctttg aattctcaaa ttccttcaaa actctttcaa ccttgccatc catccaaata
     1381 tcatcttgat cactcaaaaa aaggatatct cccctggcat gttttaaggc attctcaaaa
     1441 ttcttttgaa ccccacgttt aggaccatcc accaaataaa tattcttata ttccttttga
     1501 aatttctcaa taatatatct tgtagaatca tttgaaccat catccgaaat gatgacttca
     1561 tcactacttt gaatctgctt taaaatagag tctagttgat cttttaaata cttctcacca
     1621 ttatatgtgg ccatacatac tgaaatcatg cttgactccc cttctaattc gataggtatt
     1681 cctatcttta aaagtataac acatgaaaaa gcatcacaca aaaacacaag cacaaaaaaa
     1741 gcggatgaaa atacatttat ccgctttata taaagcgcat tcattaaatt atgtgatatt
     1801 gtcaaattca atccttgata caatttcgaa ttgaaatcaa cactnnnnnn nnnnccatag
     1861 aaatacctaa aattaaatga ccaataccag caacaccaga aatagctgca cttgaaacat
     1921 ctaaactaaa gacttgtact acaccacgaa taactaacat aactaccgtt aaattcaatc
     1981 caatatgata aaatgtcaaa tatttcaaaa ctttattatc tacaaaatga aaattctttt
     2041 cgacaacaac caacaataag aaaagcacca tttccaacat caaataatgc gtatgaacta
     2101 ccgacaatgt tgtaaattca cgataaaaca caccaccaat taaaccaagt actccatata
     2161 caattgatgc attcattaaa cgcttcatat tatcaacacc ctggcctatt atggaccttt
     2221 ctcaaataac cttcaattga tacgcatcaa aaaaatggag gtagcatcta cctccaattt
     2281 aaatcactat ttatctttta ttcatccttc tacaaacaca agaatcgcaa aaatcatttc
     2341 accttctttc cttgaccata acaatgacat tcaacccttt caaatttcca gcctattcct
     2401 aaaaatgatt aaattattct ttccttttca ttttctttcc cgctaacgca agtcctgcta
     2461 aacctaaaat accacttaat gtatagaatc ctaatgctgt ttcagttgca gtatttacac
     2521 tttctttttc tttgatttct acatcatcct ccacattctc taagtaaact ttcaaattat
     2581 tattcgcatt ttctaaatcc aatctcgcca aagtataatt cttactagcc aactctaatg
     2641 cttttacatt ctcactattc aacatgtccg cattctcaaa agcttttaaa gcctcttcat
     2701 aatttctata agcctctttc atgacgtcaa tcttttcata taaagattta actataccac
     2761 tacgtatttt attggattga agtggatgat ttgcagtgaa agaatcaaat tcttcttttg
     2821 catcctcaat ctctaatttt atagattcta attctgcaac cttaacattt attttctgaa
     2881 tactaagatc attgactgtg atactatcac acatctcagt aatcatatta tgcaaatcct
     2941 gaattctagt atttaaatgc ataattgaat cacttgtatt attcaatcta ttttgaaccg
     3001 ataaagattc ttgataataa ttatttatag atattcattt ctaccttttt gatatctcca
     3061 tctttttcac attcacttaa atctttagaa ataccatcta atagttcact agcacaatcc
     3121 aattcactct cttttgaagt cacatttgac tgcttctcaa aaactacttt tttagtatcc
     3181 tccaatactt tacccttttc ttttaataca gtttcagaaa tttttaaatt attatacgct
     3241 atctctttcc ccttatttga ttcctctaaa atctcatttt tcttatccaa ttccatctga
     3301 gcattcttca ctaaagcttc tttattattt acatttacat tcgcttcagc catctctttt
     3361 ttagcattct cgacctcttg acgtgcaaca accaaatcta acgactcttt atttgatttc
     3421 aatgccttta agttttgaat ctcattcaca atatttgaag ttgtttcttc ggaacacaaa
     3481 tcatccagat tcttttgttt ttttctaaca acactttcta aacgacccac ttcatttttt
     3541 gctgaattca atttattttc tgcatttact ttattttgct cagtaacctt ttgatcattt
     3601 gttttttgtg ttaataaagc attttgctta tacaattccc cttttaattg atttaaaatc
     3661 acattcgcat cttcataagc accatccgca ttcattaatg agtcataata agtattaaat
     3721 tgagattcaa actcatcaat cgttaataaa ctgtctagac tacattgact cgaaaatact
     3781 tgtcctgcat gtttatgatg tccatttgaa gtatacccct caatccaagc tgttccggta
     3841 ccaacatatc tagactttaa tatatttgta taatgtccag tttgaaccca aggaatatct
     3901 ggaagattac aagccttaac aatctcattt gctttattca catcaatata atttccttga
     3961 gaatctctta tatcattgat atcccatccc tttttttgaa gataatcata gacagctttc
     4021 tcctcagtat accaaccaac gtaaggatca tcataaccaa ccgcaatatt ttcactcaca
     4081 ttatacaatc gagtatggcc atattccgtt ttagccatct gatttacctg cacctgagaa
     4141 attgcaaaca tttccgcact cacctttaaa ggatttaacc ccttaaaatt atcatccgtt
     4201 gtacgtaacg tattgcccgt cttaaccata gcaattgcct ttttaaaatt ttctaaactc
     4261 gtcgcatcat tttcagcacc aacctcagta tacttaccaa tctctacact gttttcttcc
     4321 aataccttta aagcattcgt atacccatac gcctcaaaga accctttaga acccagagat
     4381 attttttgac ttacctgctc cttatctaca cgtaattgat ttacaatctt ttgttgtgca
     4441 tttacatcac tttgagcctt atctacattc gtttgttgtg cttcaacttc cttttttgct
     4501 ttatccaaag caatttgagc cttatttaaa gcctcttgca tactgttttc tgattctata
     4561 gccctatcca attgttcttt agctgcactc aattcctttt ctacaattcc tatagattca
     4621 tttataccta acaattcatt ttcttttgca tctatcaaag actttaaatt gcaaatctca
     4681 ttttctacct caacaacaca tgactcagca tttgcaacag catccacctt actttgttga
     4741 atcaacttag cattctgcaa ctcaccttgt gcaagatcta aagtgttttt tgcttctgca
     4801 acctgctttt catactccgc ctttaattct ggattactag ctccattata aatttcaagc
     4861 ttttcattat aatccgcact tgcaacatca tacgcatttt gcgcattaac cacatcactc
     4921 ttagcattcg ataactcaac acgcgcacct tctaaagtat tctgtaaatc caatacttta
     4981 gcttcagcct cttgtttagc attcaaaagc tcatttactc tatttgaagc cgcttgataa
     5041 gctagtgttg cactttccaa ttgagttttt aattcattta cactctgttc aatctgttcc
     5101 tttgaactac cgttaagtaa agattcatat ttcctttgct gttcaattaa atcatcttga
     5161 gcctgcttta actcctcgtt cgcattctct aatctagact ctaacccctt ctttttatta
     5221 ttggcatctt ttaaagcagc ttgattcgcc tctaattctt gaatctgttt ttctaaggca
     5281 gaaacaattg cttcttgagc atcgtccttt gcactcaaat aatttttttg tgccaaatcc
     5341 ctatttgaaa ctgcaaaagc ataatcattc ttattgtaag tttcatattt ttcttgagcc
     5401 tcatcaaatt tctttttagc ctcatttaca ttatccaaaa tattcttaat ttgttcctct
     5461 aacaattcct tttgagtttt ttcaacgcta acctcttcag tatctgataa attcttcatt
     5521 ggctcttctt ttgcaaatac actcatttga ccaacctgtg tcaaaaccat actagcagca
     5581 gccaaacttg ttaatgtttt attcctattt ttcatttcta taccttcttt ctaaatcaac
     5641 cacaactcaa caactatata tttctaactc atatttctgt cctcctcact atataagacg
     5701 aaacagattc ataattggtt gtatacaatt taattttttt aatacatatg ataaagacta
     5761 tccacaaagt tatataaatc catacaaaca gatttcaaat tctcaggatc attcacatac
     5821 atagcaccag cttgcgcaaa atattctgct gaatccgtag ctccatatgc ccctacacaa
     5881 tccggataat tttgatataa actctgtaat tgtgcgctat ttgaaatgcc ataatctcca
     5941 tatccagatc cacttgcaaa atcaaaaata tgacttaact catgactcac tgcatctttt
     6001 tgaccataat cctcatctac atcaatctgt aatgttattg aaaaatcatc cgatgaagca
     6061 tacgcatatg cagttcctaa tccatcatct gttacatcca ctccatcagc tattgcgata
     6121 tctataaaat tctttggctc gcaaatatgg atcatacgac aattactcaa taaaaaatta
     6181 ggttgagatt ttacaaataa atccatatat ttttgtacat caccaatttt taaagaattc
     6241 ccaggaatta catgaattgt tggtaaacca tccaaagtga tatattcatc ctcaataatc
     6301 tggttataag cctcttcttg aagtcttaac atttcattat attcattctg agcagtctta
     6361 taatctttan nnnnnnnnng acttaatacc ataaccaaag ctgctgatat taaacttaat
     6421 aatccaactt tcaaaagcca acttgtcttt ggcttatttt cattcacata atttctacac
     6481 tccaatattt gtttataaga aaattcatta gaacgcttct catacgcaat acgtctaccc
     6541 tctttgtcca aaccatcaat tctttttaac tcatcctcac ttaaagtact acggatatat
     6601 tcaaatctat attgatcagg atctacttcc ttgctataaa gaagaactac aataagcaac
     6661 atactcaata ctgcaaccgt taaacaaagt ttcaaatcaa aataattaat aagcgactga
     6721 tccataatgg taatttcctt attacccaac aaatcccgat accaatacat caatccaaat
     6781 acaactgaaa acactaaacc tgaaaacata actttacttc tattcatata aaatttctcc
     6841 ttctattttg ccttacacta ttaagacgaa gaaaaagtaa gtatggttgt ataaatgatc
     6901 aataaatttt atcttttttt tttttttt
//
LOCUS       DBMDWL010000113         6741 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4319_length_6741_cov_2.475904, whole genome shotgun sequence.
ACCESSION   DBMDWL010000113 DBMDWL010000000
VERSION     DBMDWL010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4319_length_6741_cov_2.475904"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(68..547)
                     /locus_tag="ACZPDN_08105"
     CDS             complement(68..547)
                     /locus_tag="ACZPDN_08105"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1298626.1"
                     /translation="MRKDVLSWDQYFMGMAHLSAMRSKDPNTRVGACIVNPQKRVVGL
                     GYNGFPYGCEDDEFPWARDGQFLETKYPYVVHAELNAILNSIQDLHGCTLYVSLFPCN
                     ECAKAIIQAGITCVVYESDKYDGTEGNIASKRMFHDAGVKLIQLPYDIEVETKINNR"
     gene            complement(547..1461)
                     /locus_tag="ACZPDN_08110"
     CDS             complement(547..1461)
                     /locus_tag="ACZPDN_08110"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1298627.1"
                     /translation="METRILKIDEDTKLDRLDKVLAEKLDFSRKRIKDLLDEGNVLVN
                     QKVMKASYKVCIEDEIEVSIPELDSTEVLPEPIDLDIVYEDHDIIVINKPKGMVVHPA
                     PGHYSGTLVNALLYHCKDLSGINGVMRPGIVHRIDKDTSGLLVVAKNDVAHEALSQQL
                     QDKTCHREYVAIVHKPFTHEYGTIHAPIGRDEKDRQKMAVTANNSKDAITHFSVLENF
                     SKFAYIKCQLETGRTHQIRVHMQYIEHPIAGDPKYGPRKTIQANGQLLHACALEFVHP
                     TTHEKMHFEVPLEPTFEKVLNELREEEE"
     gene            complement(1443..1937)
                     /gene="lspA"
                     /locus_tag="ACZPDN_08115"
     CDS             complement(1443..1937)
                     /gene="lspA"
                     /locus_tag="ACZPDN_08115"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1298628.1"
                     /translation="MKQGKTVVASLLIVFVTLGLDQWTKWAIVKSIKLNETVEMINHF
                     FYLTYVQNTGAGFSMFAQAGMGFFAALTLIALVAMVYMFFKTNDSRYQICLSLVFSGA
                     IGNFIDRMSLGYVRDFFSFYIFGYPFPVFNVADICITVGIALILISMFIDDKKEQAKW
                     KQES"
     gene            complement(1951..3546)
                     /locus_tag="ACZPDN_08120"
     CDS             complement(1951..3546)
                     /locus_tag="ACZPDN_08120"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR1298629.1"
                     /translation="MSDTKTMSTSARFAQIVSILRKHHLQKGMDPVKFRMILEDLGPT
                     FVKIGQIMSTRQDLFSQRYCKELMKLRSHVTPMPFSMVEQIIQDTYGDLFHEEFESID
                     EICLGSASIAQVHAATLKTKERVVLKIQRPKIYEWMERDVALLRKAVKILNLSDIVSS
                     VVDLDMVIDEFWYTAKQEMDFTIEAQFAKRFKNEYKDCKFIDAPKIYDEYTRKNILVM
                     EYVDGIEITDSKKLDELGYDRSEIADKLAFNYISQIIENGFFHADPHSGNLRIRDNQI
                     VWIDFGMMGILDANERETMKSAVRAIALKDTQKLVDCILMIGDCKKEIDYSAFCNDMD
                     KFVNQYLSVPYAQIDVAKLVQDMFSICHVYSISLPKGISMLARSMMTIEGTLTALDPN
                     MNTMKIAMSHKSSLTQIDWQKEMKSGFKRSVDALSHSIDIPVQASDVLKMVQRGQIKV
                     NLNLMGSQAPLAKIDQMVNRLIVCVLIAALVVGSSLICTTKMKPTIMGIPALGFFGYI
                     FALGMSVWLFFKMLFLHRRNKSF"
     gene            complement(3543..3683)
                     /locus_tag="ACZPDN_08125"
     CDS             complement(3543..3683)
                     /locus_tag="ACZPDN_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298630.1"
                     /translation="MKNNKDWSRLLKMILVEDKKKFNTRKVLKKSKVTNTPLHRRQDV
                     KK"
     gene            complement(3739..4209)
                     /locus_tag="ACZPDN_08130"
     CDS             complement(3739..4209)
                     /locus_tag="ACZPDN_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298631.1"
                     /translation="MRKLGVLLVASILLFVFGVGTFVYEFSQIRPHQMDLSQETQTMT
                     TSMPNSARLYTKTYLSSVGDVRVVVDEMIEDDKLQDDVLVITYPKMLHIVQDEDQLDL
                     QMDDYEMSKDLQTLFNTFRTKSYDEYYAKNNEIHISIRYGKALKDKITLVDDYY"
     gene            complement(4202..5089)
                     /locus_tag="ACZPDN_08135"
     CDS             complement(4202..5089)
                     /locus_tag="ACZPDN_08135"
                     /inference="COORDINATES: protein motif:HMM:NF046861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1298632.1"
                     /translation="MLKSEFLDLLNQKLSLINDKEKEDIILEYGTYIDDKIASGVSEE
                     EAVAGFGDVDELAKEILSAYKINTDSMDPLSSKADKTIDKVYAKLEGMFSKLGNFSMN
                     EIFHIIFDAFVLVLILWIGKLIVVDLLCRIVLSMIFSFFAGFYAITDFMLSLCRLLYV
                     CIAIYFFVKVMTKRVERYRLHANHVGVMDDIKETWNDNIKSNDLPPTPDRPLYHERKV
                     HHVESDILKVILVFAMIPVSCILIGSGIGFVVMIYVSMMYATTSLGLYCIDIAFVLGS
                     LAILLMLFRAWPKKVDTNA"
     gene            complement(5091..5405)
                     /locus_tag="ACZPDN_08140"
     CDS             complement(5091..5405)
                     /locus_tag="ACZPDN_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1298633.1"
                     /translation="MNTQFKKGVLELIVLKAIVADDLYGYQLVSRINEVIQVNEGTIY
                     PLLKRLSNDHLLESYLKESTEGPPRKYYHITSLGLARYEALLEEWTNFSESVNTYLKG
                     VK"
     gene            complement(5389..5805)
                     /locus_tag="ACZPDN_08145"
     CDS             complement(5389..5805)
                     /locus_tag="ACZPDN_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298634.1"
                     /translation="MQPRAVIYCSKHGATKELAQCLGKKYNLPVISIDHISGYSFQNI
                     PVYFCGWIRNGKIMGLTKASKLFMCVQVIGVGAVEYNEAYELKLKYKNKLVNQDFKYI
                     QSSKGLSLNPLEKMYVDVFQPSLIGKSKGVAHEYSI"
     gene            6220..>6741
                     /locus_tag="ACZPDN_08150"
     CDS             6220..>6741
                     /locus_tag="ACZPDN_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298635.1"
                     /translation="MMTTYKPAKNAQEQIEYLKTNKKITFNSIHEQQARDILYKYNYI
                     NVITPYKHHFSKFDKDGNEVKIDGKHIYERDVEFSEYFNLFKNERLQYPIIIKNILGY
                     ELRFKSIMAYRILTSTEINDENKLVAYLGSVRLRIPMNSDYSDSRLEHMNNHIKQLEK
                     SISGYADIYCFFDR"
BASE COUNT         2265 a         1301 c          905 g         2270 t
ORIGIN      
        1 atttctaact ttaattataa acccataaca agctaagcgc aaaaaagaag taacataagc
       61 tacttcttta acgattattg atctttgttt caacctcaat atcataagga agttgaatca
      121 attttacacc cgcatcatga aacatgcgtt tactcgcaat atttccttct gtaccatcat
      181 acttgtcact ttcatataca acacaagtaa taccggcttg aataatggct tttgcgcatt
      241 cattacatgg aaataatgaa acatacaaag tacaaccatg tagatcttga atactattta
      301 agatcgcatt caattcagca tgaaccacat aaggatattt tgtttctaga aactgcccat
      361 ctcgagccca aggaaattca tcatcttcac atccataagg aaaaccatta tagcctagtc
      421 caaccactct tttttgtgga ttgacaatac aagctcctac acgcgtattt ggatcttttg
      481 aacgcatagc actcaaatgt gccataccca taaaatactg atcccaactc aatacatctt
      541 tacgcattat tcttcctctt ctctaagttc atttaatact ttttcaaaag ttggttctaa
      601 cggtacttca aaatgcatct tttcatgtgt tgttggatga acaaattcca aggcgcatgc
      661 atgcaataac tggccattgg cttggattgt ttttctagga ccatattttg gatcgcctgc
      721 aattggatgt tcaatatatt gcatatgtac acgaatctga tgggttcttc ctgtttccaa
      781 ttgacattta atatacgcaa acttactgaa gttttctaga acactaaaat gcgtgatcgc
      841 atcctttgag ttgtttgcgg taaccgccat tttctgacga tccttttcat cacgaccaat
      901 cggtgcatga atcgtaccat attcatgtgt aaatggttta tgtacaatgg ccacatattc
      961 acgatgacac gtcttatctt gaagttgttg actcaatgcc tcatgtgcca catcattttt
     1021 ggcaaccaca agtaagccag acgtatcttt atcaatacga tgtacaatac ctggtcgcat
     1081 cacaccgttt ataccactca aatctttaca atgatataat aaggcgttta ctaaagtacc
     1141 agaataatgt cctggagctg ggtgtaccac catacctttt ggtttattga tcacaataat
     1201 atcatgatcc tcatatacaa tatctaaatc aataggttcg ggtaatactt cggttgaatc
     1261 aagttcagga attgaaactt caatttcatc ttctatacaa actttataac ttgccttcat
     1321 caccttttga ttgaccaaga catttccttc atccaataaa tctttaattc tcttacgcga
     1381 aaaatctaac ttttcagcta atactttatc taaacgatcc aattttgtat cttcatctat
     1441 ttttaggatt cttgtttcca tttcgcttgc tccttcttat cgtctataaa catactgatt
     1501 aatattaatg caattcctac ggttatgcaa atgtccgcca cattaaatac gggaaatgga
     1561 taaccaaaaa tatagaaact aaaaaagtct ctaacatatc ctagactcat acggtcaata
     1621 aagttaccaa tagctcctga gaaaactaaa gataaacaaa tttgatagcg tgaatcattc
     1681 gttttaaaaa acatatatac catcgcaact agggcaatta gagtgagtgc tgcaaaaaag
     1741 cccattccag cttgtgcaaa catactaaat ccagctcccg tattttgaac atatgtcaag
     1801 taaaagaaat gattgatcat ttccacggtt tcatttaatt tgatcgactt cacaatagcc
     1861 cattttgtcc attgatctag tcctaaagtg acaaatacta ttaataagga tgccactact
     1921 gtttttcctt gtttcatctt atatcccctt ttaaaacgat ttgtttcttc tatgtaaaaa
     1981 taacattttg aaaaataacc atacactcat gccaagcgca aagatatatc caaaaaagcc
     2041 aagtgcaggt atgcccataa tggtcggttt catttttgtt gtacagatta agcttgaacc
     2101 tacaaccaag gctgcaatca acacacatac aatcaaacga ttcaccatct gatcaatttt
     2161 ggcaagtgga gcttgagaac ccattaaatt taagtttacc ttgatttgtc ctctttgaac
     2221 catctttagt acatcactag cctgtacagg aatatcgatt gaatgtgaaa gtgcatctac
     2281 acttcgctta aatcccgact tcatttcttt ttgccaatca atctgcgtca aacttgactt
     2341 atgactcatc gctatcttca ttgtattcat attcggatct aaagccgtta atgttccttc
     2401 aattgtcatc atcgaacgag ctagcatact tatgcctttt ggcaaagaaa tcgaatagac
     2461 atgacatatt gaaaacatat cttgtacaag ttttgcgaca tcaatttgag cataaggaac
     2521 actcaaatat tgatttacaa atttatccat gtcgttacaa aatgcagaat aatcaatttc
     2581 tttcttacaa tcaccaatca ttaaaatgca atcgactaat ttctgtgtat cctttaatgc
     2641 gatggcacga accgccgatt tcatcgtttc cctttcattc gcatctaaga ttcccatcat
     2701 accaaaatca atccatacga tttgattatc acgaattctt aaatttcctg aatgcggatc
     2761 cgcatgaaag aaaccattct caataatttg tgaaatataa ttaaatgcca atttatcggc
     2821 aatttcacta cgatcatagc ctagttcatc caattttttt gaatctgtaa tttctatacc
     2881 atccacatat tccatcacta aaatattctt tcgtgtatat tcatcataaa ttttaggtgc
     2941 atcaataaat ttacaatcct tatattcatt tttaaatcgt ttcgcaaact gtgcttcaat
     3001 cgtaaaatcc atttcttgtt tggccgtgta ccaaaattca tcgataacca tatctaaatc
     3061 gacgacactt gaaacaatat cggatagatt taatattttt acagcttttc ttaataatgc
     3121 gacatcacgt tccatccact cataaatttt tggccgttga atctttaaaa caactctttc
     3181 ttttgtcttt agagttgctg catgtacttg cgcaatcgat gccgatccta aacatatttc
     3241 atcaatactc tcaaattctt catgaaacaa atcaccatat gtatcttgaa taatttgttc
     3301 aaccatcgaa aaaggcatag gcgttacatg agagcgaagc ttcatcaatt ctttacaata
     3361 acgctgtgaa aacagatctt gtcttgttga catgatttgg ccaattttga caaatgttgg
     3421 tcctaaatct tctaaaatca tacgaaattt tactggatcc attccctttt gaaggtgatg
     3481 ttttctaagt attgatacaa tctgggcaaa tctagcactt gttgacatcg tttttgtatc
     3541 actcattttt tcacatcctg tcttctatgt aaaggtgtat tggtcacttt actctttttt
     3601 aataccttac gtgtattgaa tttcttctta tcttccacaa gaatcatttt caacaaacga
     3661 ctccaatctt tattattttt cataacagta ttttaacata cttgttaaaa atcaaaaaga
     3721 aacagtattc actgttcctt aatagtaatc atcgaccaac gtaattttat cttttaaagc
     3781 cttaccataa cgaatactta tatgaatttc attatttttc gcgtaatatt catcatagga
     3841 ttttgttcga aacgtgttaa ataatgtttg taaatccttt gacatctcat aatcatccat
     3901 ctgcaaatcc aattgatctt catcctgtac aatatgaagc attttaggat atgtgattac
     3961 taacacatca tcttgtaact tatcatcttc gatcatttca tccacaacga cacgcacatc
     4021 acctacagaa cttaaatacg tctttgtata taagcgagcg ctatttggca ttgaagttgt
     4081 cattgtctgt gtttcttgag ataaatccat ttgatgggga cgaatttgac tgaattcata
     4141 tacaaaggtt ccaacaccaa acacaaataa taagatggaa gcaacaagta atacacctaa
     4201 tttacgcatt tgtatccacc ttctttggcc atgctcgaaa taacatcaat aaaatagcca
     4261 aacttcctag tacaaaggca atatctatgc aatagagtcc taaactggtt gtggcataca
     4321 tcatacttac gtaaatcatc acaacaaaac cgataccact gccgatcaat atgcatgaaa
     4381 ctggaatcat tgcgaaaacg agtatcacct ttaatatatc cgattcaaca tgatgcactt
     4441 ttctttcatg atataatggg cgatctggtg ttgggggcaa atcattcgat ttgatattat
     4501 cattccacgt ttccttaatg tcatccatca cacctacatg atttgcatgt aaacgatagc
     4561 gttctactct tttggtcatg accttcacaa agaaataaat cgcaatacat acatatagaa
     4621 ggcgacataa acttaacata aagtctgtaa tcgcatagaa tcctgcaaag aaagagaaaa
     4681 tcatagacaa cacaattcga cataatagat ctaccacaat cagttttcca atccacaaaa
     4741 tcaatacaag tacaaatgca tcgaatatga tgtggaagat ttcattcatc gaaaaatttc
     4801 ctagtttaga aaacattccc tccaattttg catatacctt atcaattgtt ttgtccgctt
     4861 tacttgataa tggatccatc gaatctgtat tgattttata ggcagacaaa atctctttag
     4921 ccaattcatc cacatctcca aagccggcta ctgcctcttc ttcggatacg ccacttgcga
     4981 tcttgtcatc aatataggtt ccatattcta ggataatatc ttctttttct ttatcattga
     5041 tcaatgataa tttttgattc aaaagatcta aaaattcact ttttaacatt ttattttact
     5101 cccttcaaat atgtatttac actctctgaa aaattggtcc attcttctaa taaagcctca
     5161 tatcttgcaa gtcctagact tgtgatatga taatactttc ttggagggcc ttctgttgat
     5221 tcttttaaat aagattctaa taaatgatca ttcgataaac gcttcagtaa aggataaatc
     5281 gttccttcat tcacttgaat cacttcatta attctagata caagttgata tccatataga
     5341 tcatcggcca caatcgcctt taatacaatc aattctaaga cgcccttttt aaattgagta
     5401 ttcatgcgcc acacccttac tctttcctat caaagatggt tgaaacacat ccacatacat
     5461 tttttctaaa ggatttaatg ataaaccttt gctggattga atatatttaa aatcttgatt
     5521 cacaagctta tttttatatt ttaatttcag ttcataagct tcattatatt caacagcacc
     5581 tacacctatc acttgaacgc acataaataa tttagaagct ttagtcaatc ccattatctt
     5641 tccattacga atccatccac aaaaatatac aggaatattc tgaaaggaat aacctgaaat
     5701 atgatcgatg gaaataacag gaagattgta tttttttcct aaacattgtg ctaattcttt
     5761 tgtagctcca tgtttagagc aatatataac agctcttggt tgcatattat cacctcttta
     5821 ctattatgat atacacacta ccttggattg tcaagtactg tataatatgt atttaattta
     5881 gatgaatata acattcaata ttcactttat tttaaaatat ccatcgatcg aagattttag
     5941 tagaatatag gtgtaatgat atatagtaat attcgatctg ttatcgtatt aacaaaaacg
     6001 cctagaaata tgttgattac aattcaacaa aggtcaacac atacagcaaa cattagctca
     6061 aacggaatat aaaacaatta agaagaataa taagtaattt ctagtatgta attatatctg
     6121 atttttgatt atagatttaa gaaagaaggt taaaaattat gacataaaag ataaaagaaa
     6181 caattggatt ggtattaaaa gtgatgaaaa aggagaaata tgatgacaac atataaaccg
     6241 gctaagaatg ctcaagaaca aatcgaatat ttaaagacaa ataagaaaat aacttttaat
     6301 agtatccacg agcaacaggc tcgagatata ctttataaat ataattatat aaatgtgatt
     6361 acgccttata aacatcattt ctctaaattt gataaagatg gtaacgaggt taaaattgat
     6421 ggtaaacata tttatgaaag agatgttgaa tttagcgaat atttcaattt attcaagaat
     6481 gaaagattac aatatccaat aataataaaa aatattcttg gctatgaatt gcgatttaaa
     6541 tctataatgg catatcgaat tttaacatca acagaaataa acgatgaaaa taaattagtt
     6601 gcttatttag gatccgttcg tttaagaatc cctatgaatt ctgattatag cgatagtcgt
     6661 ttagaacata tgaacaacca tattaagcaa cttgaaaaaa gtattagtgg ttatgcagat
     6721 atatattgtt tttttgacag a
//
LOCUS       DBMDWL010000114         6619 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4444_length_6619_cov_2.073489, whole genome shotgun sequence.
ACCESSION   DBMDWL010000114 DBMDWL010000000
VERSION     DBMDWL010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4444_length_6619_cov_2.073489"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="ACZPDN_08155"
     CDS             <1..576
                     /locus_tag="ACZPDN_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024117.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR1298636.1"
                     /translation="IDLYGYIDRIDSSNTSFCIFDYKSSDKDINMNEFESGLALQLAT
                     YTLAYEKESNLIPVGCFYIALRTSPEALTYGKLNYRKKVPELSVTNESDMLEAFTKNR
                     KLKGWHFSDASIYCDDIKQYMPVKKTNPALETIKSEWSQVIDSLLEDISSGQALPNHV
                     KDACKYCAYRSICRNSANEVEPKLRVEKEEE"
     gene            578..3688
                     /locus_tag="ACZPDN_08160"
     CDS             578..3688
                     /locus_tag="ACZPDN_08160"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1298637.1"
                     /translation="MDFSIPQKEAIDIRNTNVVVSASAGSGKTAVLVERLCQLVLKDH
                     ISIDSILAMTFTKDAAAEMKARLLSKLKEQPKTDYILQQMALLETASISTIDSFCLSI
                     VQNYYYKIPISYTMSKQTASSAQTRIAFENAYKHAIQDLDLNSYTKLKMYFHSFGKTE
                     EDIQKYIEDILAIINSKSEEDAKAWMENIQESYTYLNPKIMEYALKYFHNHCLAMTEI
                     LNEVIGQISKPEDYEIKKEYLSKCLDTTSYSDFKSQFELYLKNTIGFKKTIDKVDYSK
                     YQTSFKEHETRIVENLFDEAFYLKDIKNHKDLVDTLFELTNKVKLYFQLEKKKMEVID
                     FNDMEHFAYQLLQDSMIKEEMYNKYQMILVDEFQDTNELQEKIIASFCHENNVFRVGD
                     IKQSIYGFRQANPKIMQAWMEKEDDNNTPLLLQENYRSNASVIEFNNDFYSKIMNNEL
                     LGEQFKDIDIANVGTKGQMESPQYPVRFLYTEYKDEEGNKATIKKNHNENRFDLIAQD
                     IIEKHNAGMPYKSMCVLTRNHAPQEKLKAVFESYNIPAMITIDHGFFTNPAIQIVIST
                     LKALEDLTDDISLCASLMSPLFNVSFSQIANCCIGKEKGTSLYMSIKNEDFMKPFFEL
                     YANKNKTITQLLQYIYAYNNFYYASTTIQDKNNLDYLLELAGQFENQNDIHSFVLQLF
                     KDAMQDQTQEASLYGKEDDVVQVKTMHQSKGLQFPVVYILSQHEQRDKHGSSCILLDS
                     TLGLSLKGLSLDYKLKYNSIYHLALQTKKELDDLAEEMRVFYVATTRPQKELIIVDTI
                     ESIEDFYSPLNAYTLLEDESYTGWLLHTYANTSDSLVVFDKKSGLYERPLKVRQNNTP
                     YALPVYSKGSKAFSSQTASGAKIKLDWKEVSLSKNMGTIRGTLFHEIVAQCSYPYQEK
                     EIISYATSYGYTLKDHDIKQILALNSDTLYASWMKEKHIFEQSYIIEEKNQVVHGFMD
                     LVIYKEDEVIIVDFKTDAVDNEKKLIDLYAIQLQTYKKAMKKLTNVPINTYIYAFSLS
                     KCIYLE"
     gene            3738..5069
                     /locus_tag="ACZPDN_08165"
     CDS             3738..5069
                     /locus_tag="ACZPDN_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR1298638.1"
                     /translation="MVEKFFKLNEKGSNVKTELIAGLTTFLAMAYILGVNPSVLADAG
                     MDKASVFLATAISAGVASIIMGLVANYPVALASGMGVNALFAYTICGQMGFSWAAALS
                     AVFISGVIFILISVTGVRKAIINAIPVQLKLAIGAGIGFFIAFVGLKNAGIIVASPAT
                     FVTIGNLTDPTVLLAIVGLLITIALVIKNVPAAVFVGMVITAIIGIILGFMGIANMPA
                     LPERFISFDFELQAFGSCFSGISELFANPFQAFVVIFSFLFVDFFDTAGTLVAIGNRI
                     GLINDKGELENAEQALVADAFGTVFGAVLGTSTVTSFVESSSGVGVGGRTGLTACTSG
                     VCFLLAVFLSPLILSTVTNAVTAPALIVVGIMMAQQLKGIDWDDMIFAAAGFVTVIMM
                     ILMYSISNGIACGFIVYTVAMIAAGKAKEVHPTVWVLMVIFVLYFATPYIM"
     gene            complement(5096..5758)
                     /locus_tag="ACZPDN_08170"
     CDS             complement(5096..5758)
                     /locus_tag="ACZPDN_08170"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1298639.1"
                     /translation="MEKNVIITISRQFGSNGREIARKLAEYLDISYYNKEILERIAVN
                     MGVCADFFKDQNQDANGLFTIESKGLLGLRNITELSVNSQIYDMASDFIQKISQREDA
                     VIVGRCADYILKDNPDVISVFCYSDVEERIQWSIDEYQVPAKQAKKIVQEKDTQRARF
                     YEFYTNQKWGNARNYTLMINTSKMSTDEAVQLLASFYDVKKGIPTFKNAFENQYEKNN
                     TY"
     gene            5824..6606
                     /locus_tag="ACZPDN_08175"
     CDS             5824..6606
                     /locus_tag="ACZPDN_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1298640.1"
                     /translation="MKKIIFLDLDGTLWTFEKIPSSALEAIELERKNGHKIYINTGRT
                     RCEVPQFLFDMNLDGYCFGSGSEIIIDNKQVLFEPLKLRDTQFIQHIAKDSGFSLEGS
                     KTTFTSEKNRERMRQFNTDNRMGNRFLSFPDISTITDDDYNQIMKISIHFDSLSQREE
                     ILRQIPKHLTFTNFMHLGGEITHSEYNKATAIQFVKNYYKEKYETVAMGDSENDLPML
                     EYADISIAMGNGVKEVKEKADYVTDKIDNDGLYKAFKHLKLF"
BASE COUNT         2284 a         1044 c         1112 g         2179 t
ORIGIN      
        1 attgacttat atggatatat tgaccgtatt gatagttcaa atacatcctt ttgtatcttt
       61 gactataaat caagtgacaa agatattaac atgaatgaat ttgaatccgg tcttgcccta
      121 cagttagcta catacacttt ggcttatgaa aaagaatcga atttaattcc tgttggatgt
      181 ttctatattg ccttaagaac gtcaccagaa gctttaacct atggtaaatt aaattatcgc
      241 aaaaaggttc ctgaattaag tgtcacaaat gaatccgaca tgttagaggc attcacaaaa
      301 aatcgcaaac tcaaaggctg gcattttagt gatgcaagta tttattgtga tgacattaaa
      361 caatatatgc ctgtaaaaaa gacaaatcca gctttagaaa cgatcaaatc cgaatggagt
      421 caagttattg atagtctttt agaagatatt tctagtggcc aagcccttcc aaatcatgta
      481 aaagatgcat gtaaatattg tgcatataga tccatttgta gaaattcagc gaatgaagtt
      541 gaaccaaaac ttcgtgtaga aaaggaggaa gaataatatg gatttttcaa ttccccaaaa
      601 agaggctata gacattcgaa atacgaacgt tgttgtttct gcaagtgcag gaagtggtaa
      661 aactgccgtt ttagttgaac gcctatgtca attggtatta aaagaccata tttcaattga
      721 ttccatttta gccatgacct tcacaaagga tgctgcggct gaaatgaaag ctcggttgct
      781 ttcgaaatta aaggaacaac caaaaacgga ctatattctg caacaaatgg cactccttga
      841 aacggctagc atttcaacaa ttgatagttt ctgtttatct attgtacaaa attattacta
      901 taagattccc atctcttaca caatgtctaa acaaacggca tcctcagctc aaacacgcat
      961 tgcctttgaa aatgcctata agcatgcgat tcaagattta gacttaaatt catacacaaa
     1021 attaaagatg tatttccatt cttttggcaa aaccgaagaa gatattcaaa aatacattga
     1081 agatattttg gcaattatta actctaaatc tgaagaagat gccaaggctt ggatggaaaa
     1141 cattcaagaa tcttatacat atttaaatcc taaaataatg gaatatgcct taaagtattt
     1201 ccataatcat tgtctagcca tgactgaaat tttaaatgaa gtaatcggcc agatttcaaa
     1261 gcctgaggat tatgaaatca aaaaggaata tttatccaag tgtttagata caacatccta
     1321 ttcagatttt aaatcacagt tcgaattgta tttaaagaat accattggct ttaaaaagac
     1381 cattgataaa gtcgattatt ctaaatatca aacttctttt aaagaacatg aaacacgtat
     1441 tgtagaaaac ttatttgatg aagcattcta cctaaaggat attaaaaatc ataaagattt
     1501 agttgataca ttatttgaac tcacaaataa agtaaaattg tattttcaat tagaaaagaa
     1561 aaaaatggaa gtgattgact ttaatgatat ggagcacttt gcgtatcaac tcctgcaaga
     1621 ttctatgatt aaagaagaga tgtacaataa gtaccaaatg attctagtcg atgaatttca
     1681 ggataccaat gagctacagg aaaaaatcat tgcgtctttc tgtcatgaaa ataatgtatt
     1741 tagagtgggg gacattaaac aatctatcta tggatttaga caagccaatc ctaaaatcat
     1801 gcaagcttgg atggaaaaag aagatgacaa taacactcct ttgttgctac aagaaaacta
     1861 tcgttccaat gcgagtgtta ttgaattcaa taatgatttc tatagtaaga tcatgaataa
     1921 tgaattgcta ggagaacaat ttaaagatat tgatattgcc aatgtaggta caaaaggaca
     1981 aatggaaagt cctcaatatc cagttcgttt cttatataca gaatataaag atgaagaagg
     2041 caataaagct acaatcaaga aaaatcacaa tgaaaatcgt tttgatttaa tcgcacaaga
     2101 cattatagaa aagcataatg cgggaatgcc ctataaatcg atgtgtgtat taacacgtaa
     2161 tcatgctcca caagaaaaac tgaaagctgt ttttgaatcc tataacattc cggctatgat
     2221 taccattgat catggattct ttacaaatcc agccattcaa atcgtcattt ctactttaaa
     2281 agctttagaa gatttaacag atgacatttc tttatgtgcc tctttaatgt ctccattatt
     2341 taatgtatct ttttctcaaa tagcgaattg ttgtatagga aaagaaaaag gcacatcttt
     2401 atatatgagt attaaaaacg aagattttat gaaaccattc tttgagttat acgcaaataa
     2461 aaataaaaca atcacacaac ttctacaata tatttatgcg tataataatt tttattatgc
     2521 ttctacaaca attcaagata agaataactt agattattta ttagaacttg cagggcaatt
     2581 cgaaaatcaa aatgatattc atagttttgt cttacaatta tttaaagatg caatgcaaga
     2641 tcaaacacaa gaagcaagct tgtatggcaa agaagatgat gttgttcaag taaagacaat
     2701 gcatcaatct aaaggtttac aattccctgt tgtatatatt ctttcgcaac atgaacaaag
     2761 agataaacat ggaagttctt gtatcttgct tgattcaaca ttaggattat ctttaaaagg
     2821 attaagttta gattacaaat taaaatacaa ttcgatctat catttagctc tacaaacgaa
     2881 aaaagaactc gatgatcttg cagaagaaat gcgtgtattc tatgtggcga caacccgccc
     2941 tcaaaaagaa cttatcattg tagatacaat tgaaagcatt gaagattttt atagtccatt
     3001 aaacgcctat acgctattag aagatgaaag ttatacagga tggcttttac atacatatgc
     3061 caatacgagt gattctctag tggtttttga taaaaaatca ggattatatg aaagaccatt
     3121 aaaagtccgt cagaataata cgccttatgc attacctgta tattcaaaag gttctaaggc
     3181 tttttcaagt caaactgcaa gtggtgcaaa aattaaatta gattggaaag aggtttctct
     3241 ttctaaaaac atgggaacca ttcgtggaac tttatttcat gaaattgtag cgcaatgctc
     3301 gtatccttat caagaaaagg aaattatttc ttatgcgact tcttatggat atactttaaa
     3361 agatcatgac atcaaacaga ttctagcttt aaattcggat acactctatg cttcttggat
     3421 gaaagaaaaa catatatttg aacaaagcta tattattgaa gaaaaaaatc aagttgttca
     3481 cggttttatg gacttagtta tctataaaga agatgaagtg atcattgtcg attttaagac
     3541 ggatgcagta gataacgaga aaaaattaat tgatttatac gcgatacagc ttcaaaccta
     3601 taaaaaagcc atgaaaaaac taacaaatgt accaataaat acgtatattt atgctttttc
     3661 tttatcaaag tgcatctatt tagaataact tatgttacaa tggtaaagtc tttttaaaaa
     3721 agagagaagg agagagaatc gtggaaaaat tctttaaatt gaacgagaaa ggttcaaacg
     3781 ttaagacaga gcttatcgct ggtcttacaa cattcttggc tatggcctac attcttgggg
     3841 ttaaccctag tgttttagca gatgctggta tggacaaagc ctctgtattc cttgcgactg
     3901 caatcagtgc aggtgtagca agtattatta tgggtcttgt tgccaattat ccagtcgcat
     3961 tagcttcggg tatgggtgta aatgcattgt ttgcctatac aatctgtgga cagatgggct
     4021 ttagctgggc agctgcttta agtgctgtat ttatttcagg tgttatcttt attcttattt
     4081 ctgttactgg tgttcgtaaa gctattatca atgcgattcc agtacaatta aaattggcaa
     4141 tcggagctgg aattggcttc tttattgcgt ttgttggttt aaaaaatgca ggtattattg
     4201 tagctagtcc tgctacattt gtaacaattg gaaacttaac agatccaact gtattattag
     4261 ctattgttgg tttattaatt acgattgcgt tggttattaa aaatgttcct gcagctgttt
     4321 ttgtaggtat ggttatcaca gctattattg gaatcatcct tggattcatg ggaatagcta
     4381 atatgcctgc tttacctgaa cgtttcattt cgtttgattt tgaattacaa gcctttggat
     4441 cttgcttctc aggaatctct gaattgttcg caaatccatt ccaggctttc gttgttattt
     4501 tctcattctt gttcgttgac ttctttgata cagcaggaac acttgttgcg attggtaacc
     4561 gtattggttt aatcaatgat aaaggtgaac ttgaaaatgc tgaacaagca ttggttgcag
     4621 atgcatttgg cacagtattt ggtgctgttt taggtacgag tacagttaca agctttgttg
     4681 aatctagctc tggtgttggt gtcggaggac gtacaggttt aactgcatgt acatcaggtg
     4741 tttgtttctt actagccgta ttcttatcac cacttatctt aagtacagtt acgaatgcag
     4801 ttactgcacc agcattgatt gttgttggta ttatgatggc tcaacaatta aaaggtattg
     4861 actgggatga tatgattttt gccgctgctg gatttgtcac tgttattatg atgatcttaa
     4921 tgtattctat ttcaaatggt atcgcatgtg gatttatcgt ttatacagtt gctatgattg
     4981 ctgcaggtaa agcaaaagaa gttcacccaa ctgtttgggt attaatggtt atcttcgtac
     5041 tatactttgc aacaccttat attatgtaaa agaaaaagtc ggatttccga cttttttaat
     5101 acgtattatt cttttcatat tgattttcaa aggcattctt aaaagttggg atgccttttt
     5161 ttacatcata aaatgatgct agtaattgta ctgcttcatc tgtgctcatc tttgatgtat
     5221 tgatcatcaa tgtataatta cgtgcatttc cccatttttg attggtatag aattcataaa
     5281 atcttgccct ttgcgtatct ttttcctgca caattttctt ggcttgttta gcaggcactt
     5341 gatattcgtc aatcgaccat tgaatacgtt cttctacatc actgtaacag aatacagaaa
     5401 taacatccgg attatctttt aaaatataat ccgcacatct tcctacaata acagcgtctt
     5461 ctctttggga aatcttttga ataaaatcac tcgccatatc ataaatttgt gaatttacac
     5521 ttaattccgt aatatttcta agtcctaaaa gacctttaga ttctattgta aataatccat
     5581 tcgcatcttg attctggtct ttaaagaaat ctgcacaaac acccatattc actgctattc
     5641 tttccaaaat ttctttatta taataactaa tatccaaata ttcagctaac ttacgtgcaa
     5701 tttctcttcc atttgaacca aattgtctag atatcgttat aatcacattc ttttccatgc
     5761 caataccctc ttttttctta tatcatacaa taatatgtta aaatattgta gtggaggaag
     5821 gttatgaaaa aaattatttt tttggattta gacggaactc tatggacatt tgaaaagatt
     5881 cctagttctg cattagaagc gattgaacta gaaagaaaaa atggtcataa aatttatata
     5941 aacacaggaa gaacacgttg tgaagtccct caatttttat tcgatatgaa tttagatggc
     6001 tattgttttg ggtctggtag tgaaatcatc atagataata aacaagtcct ttttgaacca
     6061 ttaaaactaa gggatacaca atttattcag cacattgcca aagattcagg attctcacta
     6121 gaaggcagta aaacaacatt tactagtgag aaaaacagag aaagaatgcg ccaatttaac
     6181 acagacaatc gcatgggaaa tcgtttcttg tcttttccag atatttcaac aattaccgat
     6241 gatgattaca atcaaatcat gaaaatcagt attcattttg actcactttc gcaaagagaa
     6301 gaaatattaa gacaaattcc taagcattta acattcacaa acttcatgca tctaggcgga
     6361 gaaatcacac attcagaata caataaagct acggctattc agtttgtcaa aaactattat
     6421 aaagaaaaat atgaaacagt tgcaatgggg gattctgaaa atgatttacc aatgttagaa
     6481 tatgctgata tttctattgc gatgggaaac ggtgtcaaag aggtaaaaga aaaagccgat
     6541 tatgtcacag ataaaattga caatgacggc ttatacaaag cttttaaaca tttaaagcta
     6601 ttttagaatc agaaatgat
//
LOCUS       DBMDWL010000115         6564 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4493_length_6564_cov_2.249575, whole genome shotgun sequence.
ACCESSION   DBMDWL010000115 DBMDWL010000000
VERSION     DBMDWL010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4493_length_6564_cov_2.249575"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="ACZPDN_08180"
     CDS             complement(<1..516)
                     /locus_tag="ACZPDN_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR1298641.1"
                     /translation="MQAIMRYELVINEALRSALMFDTPDEQINEFIRFFGKHIGCDRI
                     YIFEDNKKKHVTNNTYEWCSEGIQPEIDFLQGVDMDIIDWWYKAFDKKENVIIQDVET
                     IKKDHAYTYNTLKVQNVTRLVVCPIRYKDEISGFFGVDNPPIDNHSSLTTFFDMIATL
                     VVSFLKIRNSQN"
     gene            complement(647..1279)
                     /locus_tag="ACZPDN_08185"
     CDS             complement(647..1279)
                     /locus_tag="ACZPDN_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1298642.1"
                     /translation="MKKNCTEVVFILDRSGSMCGLEKDTIGGFNSLINKQKKQDGEVL
                     VSTVLFNDSSVVIHDRKPIEKVRLMTNKDYEVYGCTALLDALGSSIRHIGNIHKYARI
                     EDVPEHTMFVIITDGMENASRYYSKERVKSMIEYEKEKYGWEFLFLAANMDAIDTASQ
                     YGIDKDHAVNYQCDSVGTRLNYEVLDEAIQCICSGVPLSKSWKSKIEKRK"
     gene            complement(1351..2283)
                     /locus_tag="ACZPDN_08190"
     CDS             complement(1351..2283)
                     /locus_tag="ACZPDN_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR1298643.1"
                     /translation="MPIKIIRQDITKLKVDAIVNTTNPSLDATGGLDHYIHQLAGKKL
                     DVECRRIGKLKVGQACLTSGYKLCKYIIHTAGPVWSENNHLEEVLLKSCYLSSLRLAD
                     SYKLKSIAFPLISSGNNQFPKELALQVAMHSIVSFLTDHEMMVYLVVYDRNSYKISSE
                     LFDSIEAYIDDYYEDEHMLVCNSIISPRFSLIDALNQMDESFSTMLLRKIDESGMSDV
                     ECYKKANIDCKLFSKIRSNPNYKPSKQTVIAFALALELSLEDMEDMLNKAGFSLSHSN
                     KFDIIVEYFVSQGNYNVFEINEALFAFDQSLIGV"
     gene            complement(2313..4388)
                     /locus_tag="ACZPDN_08195"
     CDS             complement(2313..4388)
                     /locus_tag="ACZPDN_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HIR1298644.1"
                     /translation="MRDFDSNRIYEILGEEFKNIYIVDCETQQIHAFKETDVLPGFSA
                     HMLYNDAMNQYIDHCVYEKNRDMLRIALSFNSLYTRLENAEHFNIHYQSKTDSEIHFM
                     YTHFGCIKDHDKITQIVIGFANEDLDIRRNNLDMINNVITPSGLRRKVLIVEDNELNR
                     EMLKATLEDEYDVLEAVNGEDGLQILSQNYKDISIVLLDVVMPVCDGFEFLTRQKSDS
                     LLASVPVIVTTGNNSQEDELKCLGLGAVDFITKPYNTRIVMSRIDSVIKLRESSMTLA
                     AIEKDELTGLYTRQAFYHHARTLTNFMTDEIFNIVVLDVADFKLINGTYGTKKGNEVL
                     IYLANAFKYYIKNGLLTRYEGDQLLALFHSKEKIDVDRIENNLNKIMEEAPIPNIRVK
                     LGIYENVDTSLQIPIMCDRALMAVKSISKDFKQNIAFFTQEMNQKLLAQRQMENDFKD
                     AIKNREFVVYFQPKYDVSSEQIVGAEALVRWQKEDGTLISPGLFIPLFESDGLVVQLD
                     EYVFETVCRFQKDRMKQGLPILPISVNLSRASIHFSDVVQRYVNIIKENEIPFSCVPI
                     ELTESAALYSQQILEITNQMVNAGFKLHMDDFGSGYSSLTTLNELKFTTVKLDKSLID
                     YIAKPRGMKIVRQTIDLGHGLDMKVVAEGIETKEQRDCLIEMNCDEIQGFYYSKPLKQ
                     DDFIEKLRA"
     gene            complement(4490..4702)
                     /locus_tag="ACZPDN_08200"
     CDS             complement(4490..4702)
                     /locus_tag="ACZPDN_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HIR1298645.1"
                     /translation="MGVSIFYTIVGAIIGGLVTFYFTRKKFEKEIKENPPINEKMIRA
                     MFLQMGRKPSEAQIRQIMKSVNANRK"
     gene            complement(4747..5616)
                     /locus_tag="ACZPDN_08205"
     CDS             complement(4747..5616)
                     /locus_tag="ACZPDN_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1298646.1"
                     /translation="MSKKQANLVCLIVAAIWGGGFIATDAALQTFDPFTVLMIRFMGA
                     SLVSIVVCLCSHVKVSKQALFRGSISGALMYLAFAFQTFGLAMTNTGQNAFLTAVNVV
                     LVPYIVWLLWKKKPSKRQLGASVICLVGIACLSLSKGYFQFSFGDCLSLICALFFACH
                     IISLEYATENSNSITINAVQMSVAALLSIPFALSLETIPQNISMHAYMSCGYMILVAT
                     FLAFQLQTLAQKYTDASSASVLLCTESLFANVFGFVLLHEAKSPIMILGGLLIFFSVI
                     LTEYSGQFVLSKA"
     gene            complement(5613..>6564)
                     /locus_tag="ACZPDN_08210"
     CDS             complement(5613..>6564)
                     /locus_tag="ACZPDN_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR1298647.1"
                     /translation="TGDGYIKRVSMRSYNASSDSMTQRKDTDSLVCYGASHTLQTLVF
                     FTNRGTYGYIPVYQIEEAKWKDLGNHISNYIKMDSSEKIIAAYVLDAFKTGVHVVSAS
                     RHGMIKRTELKEFEVSRSNKTMTCMKIGAMDEMISVSLSYKPDDQVILVSEQGYGLMY
                     PVEQIPLVSNKSKGVKAMNLAKDDCVASICIRKNRDEQVLISTTTLSLKRMKQLEIAP
                     LNRPAKGNRICKLVKSNPNVIFNLQMVDLNTKIEIYTDEILTFEAKEISLMNAQSTFS
                     MPYGKVESFEWICPLENVLDGAWVKQEDFKQPSLFEENNA"
BASE COUNT         2216 a         1312 c          837 g         2199 t
ORIGIN      
        1 attttgtgaa ttacgaatct ttaaaaaaga tacaaccaac gttgcgatca tatcaaaaaa
       61 tgttgtcaaa cttgaatgat tatcaatcgg cggattatca acaccaaaga atccactaat
      121 ttcatcttta tagcgaatag gacaaacaac tagacgtgta acattttgta ctttcaatgt
      181 attatacgta tacgcatgat cctttttaat tgtttctaca tcttgaatga tcacattttc
      241 tttcttgtca aaagctttat accaccaatc aataatatcc atgtccacgc cttgtagaaa
      301 atcaatttcc ggttgaatac cttcactaca ccattcataa gtattatttg tcacatgctt
      361 ttttttatta tcttcaaaaa tataaatgcg atcacaccca atgtgttttc caaaaaaacg
      421 aataaactca ttgatctgct catccggtgt atcaaacata agtgcagaac gtaatgcttc
      481 attgatgacc aactcatagc gcataattgc ctgcataagt tacctgcctt tctttaagaa
      541 atatttcaat tatctaacca cattatatct tttaaacggt tatacatcag ttatatttta
      601 taaaaattta gtcaattaaa caagaaaaga accacatagt tggttcttac ttacgctttt
      661 ctatttttga tttccaagat tttgacaaag gtacacccga acatatacac tgaatggctt
      721 catccaatac ttcataattc aaacgagtac caacagaatc acattgataa tttaccgcat
      781 gatccttatc aattccatat tgacttgccg tatcaatcgc atccatattg gctgcaagaa
      841 ataagaattc ccagccatat ttttctttct catattcaat catcgacttg actctttcct
      901 ttgaataata tcgactcgca ttttccattc catccgtaat aatcacaaac atcgtatgct
      961 ctggaacatc ctcgatccga gcatatttgt gaatatttcc aatatggcga atacttgaac
     1021 ctaatgcatc taataatgcg gtacaaccat atacctcata atccttattt gtcataagtc
     1081 taactttttc aatgggctta cgatcatgaa tgaccacact tgaatcatta aataagacgg
     1141 tcgaaactaa aacttctccg tcttgtttct tctgtttgtt gattagagaa ttaaaccctc
     1201 caattgtatc cttttctaaa ccgcacatcg atccacttcg atctagaata aaaactactt
     1261 ctgtacaatt ttttttcatc actgtatcct ccttgattga ttacagtata gtttttgttt
     1321 ctatcaaaat ggtcgcctac aaagcgacat ttatacgcca atcaaacttt gatcaaacgc
     1381 aaacaacgct tcattgattt caaagacatt ataatttcct tgggatacaa aatattcaac
     1441 aataatatca aacttattgg aatgcgataa actaaaaccc gccttattta acatatcttc
     1501 catatcttcc aaacttaact caagtgcaag cgcaaaggca atcacagttt gtttcgaagg
     1561 tttgtagtta ggattcgaac gaatcttaga aaacaatttg caatcgatat tggccttttt
     1621 atagcattcc acatcactca taccactctc atcaattttt cttaaaagca ttgtcgaaaa
     1681 actttcatcc atttgattta aagcatctat taaagaaaat cttggagaaa tgatcgaatt
     1741 gcataccaac atatgctcat cctcataata atcatcaata taagcttcaa tggaatcaaa
     1801 taattcagaa ctaattttat atgaatttcg atcataaaca actaaataca ccatcatttc
     1861 atgatctgtt agaaaagaga caatactatg cattgccact tgtaaagcta actcttttgg
     1921 aaattgatta tttccggatg aaatcaaagg aaatgcgatt gatttcaatt tatatgaatc
     1981 cgccaatctt aaacttgata agtaacaaga tttcaacaag acttcttcca aatgattatt
     2041 ttcactccat acaggaccag ctgtatgaat aatatactta cacaatttat atccacttgt
     2101 aagacaagct tgtcctactt tcaattttcc tattcttcta cactctacat ctaatttttt
     2161 acccgcaagt tgatgtatgt aatgatctaa gccacctgtc gcatccaaac ttggatttgt
     2221 cgtattcaca atcgcatcca cttttaattt tgtgatatcc tgacgaatta tttttattgg
     2281 cataagccct cctatacaac aaaagagctt tattaagctc ttagtttttc gataaaatca
     2341 tcttgtttta aaggctttga ataatagaag ccctgaattt catcacaatt catttcgatc
     2401 aaacaatccc tttgttcttt ggtttcaata ccttccgcaa ccactttcat atctaaacca
     2461 tgccccaaat caatcgtttg tcgcacaatt ttcattccac gtggtttggc aatatagtca
     2521 attaaactct tatccaattt aaccgttgtg aatttcaatt catttaaagt tgttaatgaa
     2581 gaatatccgg atccaaaatc atccatatgt aatttaaagc ctgcattgac catttgattt
     2641 gtgatttcaa gaatttgttg cgaatataaa gctgcagatt ccgtcaattc aatcggaaca
     2701 caactaaaag gaatttcatt ctcttttatg atattgacat atctttgtac cacatcacta
     2761 aagtgaatac ttgcacgaga tagattgaca gaaataggaa gaatcggtaa accttgtttc
     2821 attcgatctt tttggaatcg acatactgtt tcaaatacat attcatcaag ttgaacaact
     2881 aaaccatcac tttcaaataa tggaataaat aaacctggtg aaatcaatgt tccatcttct
     2941 ttttgccaac gaacaagtgc ttctgcacca acaatttgtt cagatgaaac atcatatttg
     3001 ggttgaaaat agactacaaa ctctctattt ttaatagcat ctttaaaatc attttccatt
     3061 tgtctttgcg caagtaattt ttgattcatt tcctgcgtaa agaaggcgat attctgttta
     3121 aaatcttttg aaatactctt cacagccatt aatgcacgat cacacatgat tggtatctga
     3181 agacttgtat ctacattttc ataaattcct agtttcacac gaatatttgg aataggtgct
     3241 tcttccataa tcttatttaa attgttttcg atacgatcca catcaatttt ttctttacta
     3301 tggaataaag ccaataattg atctccctca tagcgcgtca acaatccatt tttgatatag
     3361 tatttaaacg cattcgcaag gtaaatcaaa acttcatttc ccttttttgt tccgtaagtt
     3421 ccattgatca atttaaaatc agccacatct aaaacaacga tattaaaaat ttcatctgtc
     3481 ataaaattag tcaatgtacg agcatgatga taaaaagcct gtcttgtata tagacctgtc
     3541 aactcatctt tttcaatggc tgccaaagtc atacttgatt ctcttaactt aataacacta
     3601 tcaattcgac tcattacaat tcttgtatta tagggctttg taatgaaatc aacagcacct
     3661 aaacctaaac atttcaattc atcttcctgc gaattatttc ccgttgtcac aatgactgga
     3721 acactagcta acaaagaatc tgatttttga cgagttaaga attcaaatcc atcacacaca
     3781 ggcattacaa catctaataa aacaattgaa atatctttat aattctgaga tagaatttgt
     3841 aaaccatctt ccccatttac agcttctaat acatcatatt catcctctaa agtagccttt
     3901 aacatttctc gatttaattc attatcttca acaattaaaa cttttcttct caaaccactt
     3961 ggtgtaatca cattgttgat catatccaaa ttatttcttc gaatatccaa atcttcattc
     4021 gcaaaaccaa tgacaatttg agtaatttta tcatgatctt taatacaacc aaagtgtgta
     4081 tacataaaat gaatttcgga atccgtttta gattgataat gaatattaaa atgttccgca
     4141 ttttctaaac gagtataaag ggaattaaac gaaagcgcaa tacgtaacat atcacgattc
     4201 ttttcgtata cacaatgatc aatatattga ttcatcgcgt cattataaag catatgtgcg
     4261 gaaaaaccag gcaacacatc tgtttcctta aaagcatgaa tctgttgagt ttcgcaatca
     4321 acaatataga tgtttttaaa ttcttcgcct agtatttcat agatgcgatt tgaatcaaaa
     4381 tctctcataa taaccctctt ctaaaaatat ataactaacc aacagtatta tataaccaaa
     4441 atttaattta ggcaacgaaa caaaaaaagt gtcttgcgac actttatcat tatttacggt
     4501 ttgcgtttac cgatttcatg atttgacgaa tttgtgcttc actaggttta cgtcccattt
     4561 gtaagaacat tgcacgaatc atcttttcat taataggtgg attttcttta atttcttttt
     4621 caaacttctt acgagtgaag tagaaagtaa ccaagccacc aatgatggct cccactatcg
     4681 tataaaaaat tgatacaccc atattttagt caacctcact atctactcat ctattctact
     4741 atctttttaa gcttttgaca atacgaattg accactatat tcagttaaaa tcactgaaaa
     4801 gaagatcaaa agtccaccta aaatcataat tggactcttt gcttcatgaa gcagaacaaa
     4861 gccaaataca ttcgcaaata aagattctgt gcataaaagt acacttgcac tcgatgcatc
     4921 tgtatacttt tgtgccaagg tctgtaattg aaatgccaag aatgttgcca ctaaaatcat
     4981 atagccacaa ctcatgtaag catgcattga aatattttgt ggaattgttt ccaaagacaa
     5041 tgcgaaagga attgaaagta aagcagcaac actcatctgt acggcattga tcgtaataga
     5101 attggaattt tcagttgcat actccaaaga aataatgtga cacgcaaaaa acaaggcaca
     5161 tatcaaagat aaacaatctc caaaactaaa ttggaaatag ccttttgata agcttaaaca
     5221 agcaatgcct actaagcata ttacactagc gccaagttgt cttttacttg gcttcttttt
     5281 ccataaaagc catacaatat atggaacaag cacaacattg acagctgtca aaaatgcatt
     5341 ttgacccgta tttgtcatgg ccaagccaaa agtttgaaat gcgaatgcca aatacattaa
     5401 cgcccctgaa atgcttcctc gaaatagtgc ttgttttgaa actttaacat gactacaaag
     5461 acaaaccaca atactaacta ggcttgcacc cataaaacga atcattaaaa cggtaaaagg
     5521 atcaaacgtt tgtaaagcag catctgtggc aatgaatcca ccaccccaaa tggctgcaac
     5581 aattaagcaa actaaatttg cttgtttttt actcatgcgt tattctcctc aaataaagaa
     5641 ggttgtttaa aatcttcttg ttttacccaa gctccatcca atacattttc aagagggcag
     5701 atccattcaa atgattcaac ttttccatat ggcattgaga aagttgactg tgcattcatt
     5761 aaactgattt ctttcgcttc aaatgtaagg atttcatctg tatatatttc aattttcgta
     5821 tttagatcca ccatttgtaa attgaaaata acatttggat tcgattttac caacttacaa
     5881 atacgatttc ccttagccgg acgatttaat ggtgcaattt caagttgttt catacgcttt
     5941 aaagaaagtg tggttgtaga aatcaatact tgttcatctc tatttttacg aatacaaata
     6001 gaagcaacac aatcatcctt tgcaagattc attgccttaa caccttttga tttattagaa
     6061 accaatggaa tctgttcaac cggatacatt aaaccatatc cttgttcact tactaaaatc
     6121 acttgatcat ctggtttata agatagagat acagatatca tttcatccat agcaccaatt
     6181 ttcatacaag tcatcgtttt atttgaacga gaaacctcga actcttttaa ttctgtacgt
     6241 ttgatcatgc catgacgact tgcactcaca acatgtaccc ctgttttaaa agcatctaat
     6301 acataggccg caatgatttt ttcacttgaa tccatcttaa tataatttga gatatgattt
     6361 cctaaatctt tccattttgc ctcttcaatt tgataaacag gaatatatcc atatgttcca
     6421 cgatttgtaa agaaaaccaa agtctgtaat gtatgactcg ccccataaca aaccaaagaa
     6481 tctgtatctt ttctttgtgt cattgaatca cttgatgcat tatagctacg catagaaaca
     6541 cgtttaatat atccatctcc agtt
//
LOCUS       DBMDWL010000116         6554 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4501_length_6554_cov_2.581590, whole genome shotgun sequence.
ACCESSION   DBMDWL010000116 DBMDWL010000000
VERSION     DBMDWL010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_6554_cov_2.581590"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="ACZPDN_08215"
     CDS             <1..690
                     /locus_tag="ACZPDN_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1298648.1"
                     /translation="SELAKYGYSSFEQLNDYCITSVKEKEMNKKYITKHFDEVKKAWE
                     EKKPRTVSIIKMEVSDADNLTETETKKKNNIDKALEKESFADVAKAFSEDSNTANKKG
                     FAGYVDSDSTSSETSSDSSSTSTVPADVVTAAINLKKGETSDWITVTNSSGTSLYRVY
                     VKETDSNKIFNAKNDTVSNQALYAILNSDSSLSYKILDSYAKKLDIKFNDKDTKKKFD
                     NYVKTTYGGDQ"
     gene            690..1589
                     /locus_tag="ACZPDN_08220"
     CDS             690..1589
                     /locus_tag="ACZPDN_08220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1298649.1"
                     /translation="MKKTPILLVCAAMMLSACSGATATIKDKDEAIMTIGNTTYTKGD
                     EYDLLKISTGTDLTMELVKQAIYKQEVKVTDEMKEKAQEQIDNYKENMSDFESQIKSL
                     GYSSKKQYMNKVLIPSLQASELTEKYFTDAKKDVQNTYKPSKARIIQCENKATAKKAL
                     KALKDGTDPEEVASQYMVDSATYSGKETLITTKKTDISTRMINKLYKTKKAGVIDEIF
                     TNESSGTTYAYVAVLVTNTYKDIKDEVYTTLSSDDDVKKACLVYYLKKYNFEVHDQDV
                     FDNLKANNPEYLVSRPDLAKSKD"
     gene            complement(1612..1884)
                     /locus_tag="ACZPDN_08225"
     CDS             complement(1612..1884)
                     /locus_tag="ACZPDN_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HIR1298650.1"
                     /translation="MKDLLPIGSVVTLKEGTKRLMIIGRLQQNVRTKKVYDYAGCLWP
                     EGYMDKDSCYVFNHEDIDCLYYIGLQDIEEFNFRFELDEMRKKTSQ"
     gene            complement(1896..2690)
                     /locus_tag="ACZPDN_08230"
     CDS             complement(1896..2690)
                     /locus_tag="ACZPDN_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298651.1"
                     /translation="MKADSLSCKNLSAQYNLLASRNIALKNPALKERQNRIAKEIMDI
                     SNYLSKSANLIENTENKQLLFQPGKHNFTKYVNLNLVSQLTKQSRYSYGSIGYTASLK
                     EWNKNYTRDYFHVGANAAILDGEIKSSISARLWKNKKFDPRVVLQGEASVALLSSTVN
                     ARIGNSKVYASARATGQVGVAYANCKAVFSKKVQSFEAGVGVAALRGETRCVLNILGA
                     KVTLTAQGSVGSAEANFSYHFSNREWEIGSKLGFIAGLGFKINVSY"
     gene            complement(2843..3127)
                     /locus_tag="ACZPDN_08235"
     CDS             complement(2843..3127)
                     /locus_tag="ACZPDN_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1298652.1"
                     /translation="MTQIQISSEDVMAYSKQVDAYANEIRTVFNEIESKMYYVESIWQ
                     SPASKYFMEQFQTLNPAMMSYLETLTRFSTYLHQTANLYQENEQNLMHHG"
     gene            complement(3139..3432)
                     /locus_tag="ACZPDN_08240"
     CDS             complement(3139..3432)
                     /locus_tag="ACZPDN_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HIR1298653.1"
                     /translation="MKISVDYSEVYQASVYVKNKADSYNDLIQNLYKRVEQMQSIWQG
                     VDHLAFQNQLEEFRPSLNEMYQVILEYSNVLKQTASVYEKLQQDRVQQAKLLL"
     gene            complement(3441..3647)
                     /locus_tag="ACZPDN_08245"
     CDS             complement(3441..3647)
                     /locus_tag="ACZPDN_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298654.1"
                     /translation="MNEIGIYLSFDRKMIFVSESYRVQDFFDEFLEYEVRYVFNVTVM
                     CFLDLNKTFKENHIAHCDMLIYGR"
     gene            complement(3640..>6554)
                     /locus_tag="ACZPDN_08250"
     CDS             complement(3640..>6554)
                     /locus_tag="ACZPDN_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013727.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HIR1298655.1"
                     /translation="FHFKIEKDVLTDLDSLNGTYVNTKRVHSYKLNMQDEIVIANVRM
                     IYFKNYLLVDMVDNPFENKDLKREEPMFYPSNLNVQLPTIQKWDIQMPQIHRVVCKQS
                     LFSAIGPSCMIASSGFISSILIGYLQKQSVETLFMSMISSLTMAVTFMIYGLYNRNYQ
                     YKLSVKENQKSLDMYDGYLKRMYEKEGLLKNDFEKKVNLYIKKYVDTFKHETKDIVYV
                     GCTKLKWCAISYREVSYEYALNDLIQKRERLIESLNSKILQPVFLKKGEVCWIKGNIE
                     ESLILFENYLWYSNSMRKWLWLQDFDEIKDVLLNPFCIVNEKSNSEFIVVTRDLKCQP
                     KEYYCLIYIGKKEPSFHYDYCIQNICISNINKDQKRYLLQSQNIDFYQQLIENGPIRK
                     ERYVMRVPVGMRESNQIVYMDFRKYGSHGLVAGMTGFGKSEFISFLLMMMIWHNAPSQ
                     FQYILIDFKGGAFGQPFYEFAHCAGIVTNLDAQSMERFFMSMNYELEKRQRLFLAAKV
                     ADINAYNETHTLSHLWIFVDEFAQLKTRFPQFMSQLQEIARIGRSLGIHLVLSTQKPM
                     GIIDDQVMSNTSWKVCFHVNNVQDSREILQNEKAYTLKNPGDMVLQTKNESLECKSFY
                     LQKYVDEKSWREVNERKEVIQSKQHLSKRVIDALKEKINVLKEEKSWVLLPKKVSKED
                     FGILDLPFKQKQCELVFDHLQLIYTKSMDIVYSLINYFKDETIYVYGTHVLNDYVDFN
                     FFKSRCFHQILSGVCIVFEDENLDLSLLNENVRAFIITENENTRLKWIQSKYVFDVDS
                     LDDKRIYFDTYQLPSYLNMTCYENTYVECIYNRVIESNLKRRACKLFSYRKERNCIGF
                     ESQSDRPVFIDEKRKLYILYMHEEYKCKAYKLCENLSHLKICSDLGSNSDIYVICVDF
                     KQLTNEVFQKALYQAQVLWVGYGLKEYGYTLRRKLPYESFDCVFFKDQNEGIGVYE"
BASE COUNT         2327 a         1261 c          831 g         2135 t
ORIGIN      
        1 tcagaactag ctaaatatgg atattcatcc tttgaacaat taaatgacta ttgcataaca
       61 agcgttaaag aaaaagaaat gaataaaaaa tatatcacaa aacattttga tgaagtaaaa
      121 aaagcctggg aagaaaaaaa acctagaact gtttctatca ttaaaatgga agtaagtgat
      181 gcagataatt taacagaaac agaaacaaaa aagaaaaata acattgataa agctttagaa
      241 aaagaaagtt ttgcggatgt cgcaaaagca ttctctgaag attcaaatac cgcaaacaaa
      301 aaaggttttg caggatatgt agattcagat tcaacaagct cagaaacaag ctcagactca
      361 agttcaactt ctactgtacc tgctgatgta gttacagctg ccattaatct taaaaaaggt
      421 gaaactagcg actggattac agttacaaat tcaagtggca caagcttata tagagtttat
      481 gtaaaagaaa cagattcaaa taaaatcttc aacgcaaaaa atgacacagt ttcaaaccaa
      541 gcattatatg cgatcttaaa cagcgactct tctctttcat acaaaattct tgattcatac
      601 gcgaaaaaat tagatattaa attcaacgat aaagacacta agaaaaaatt cgacaactat
      661 gtaaaaacga cttatggagg tgatcaataa tgaaaaagac acctatacta cttgtatgtg
      721 cagccatgat gttgagcgca tgttctggag caactgccac aattaaagat aaagatgaag
      781 ccatcatgac cattggcaat acaacatata caaaaggcga tgaatatgat ttattaaaaa
      841 tttcaacagg tacagatctt acaatggaac ttgttaagca agcaatctat aaacaagaag
      901 ttaaagtaac cgatgaaatg aaagaaaaag cccaagaaca aatcgacaac tataaagaaa
      961 atatgtccga ttttgaaagt caaattaaat ctcttggtta ttcaagcaaa aaacaatata
     1021 tgaataaagt tttgattcca agtttacaag caagtgaatt aacagaaaaa tatttcacag
     1081 atgctaaaaa agatgttcaa aacacatata aaccaagtaa agctcgtatc attcaatgtg
     1141 aaaataaggc aacagctaaa aaagcattga aagctttaaa agatggaaca gaccctgaag
     1201 aagtagcttc acaatatatg gtcgattcag ccacatattc aggaaaagaa acattaatta
     1261 caacaaagaa aacagatatt tccactcgaa tgatcaataa gttatataag acaaaaaaag
     1321 ctggagtcat cgatgaaatt ttcacaaacg aaagcagtgg aacaacatat gcttacgttg
     1381 ccgtattagt aacgaacaca tacaaagata ttaaagatga agtctataca acattaagca
     1441 gtgatgatga tgtcaaaaaa gcttgtcttg tatattactt aaagaaatac aattttgaag
     1501 tgcatgatca agatgtattc gacaatttaa aggcaaacaa tccagaatat ctagtttcta
     1561 gaccggatct tgcgaaatct aaagactaat aaaacgccaa tagacaaatg tctattggct
     1621 tgtttttttt ctcatttcat cgagttcaaa ccgaaaattg aactcttcta tatcctgcaa
     1681 tccaatatag taaagacaat caatatcttc atgattaaat acgtaacaag aatccttatc
     1741 catatagcct tctggccata aacatcctgc ataatcatac actttctttg tccttacatt
     1801 ttgttgaaga cgcccaataa tcattaatct tttagtcccc tcttttaaag tcactacact
     1861 tccaattggt aataaatctt tcatttagcc tccttttaat agctcacatt gattttaaag
     1921 cccagccccg caataaaacc aagcttagat ccaatttccc actctctatt tgaaaaatga
     1981 tatgaaaagt ttgcctcagc acttccaaca ctcccttgtg cagttaaagt tacttttgca
     2041 ccaagaatat ttaaaacaca acgcgtttct cctcttaagg ctgcaacacc aacaccagcc
     2101 tcaaacgact gcaccttttt agaaaatacg gccttgcaat tcgcatacgc cacaccgact
     2161 tgtcctgttg cacgagcact cgcatacact tttgaattac caatacgtgc attcaccgtt
     2221 gatgataata aggcaacact agcctcacct tgtaatacaa cacgaggatc aaatttttta
     2281 tttttccata accgagcact tatactcgat ttaatttctc catctaatat agccgcattg
     2341 gcccccacat gaaaataatc cctcgtatag tttttattcc attcctttaa tgaagctgta
     2401 tatccgattg agccataaga atatcgactt tgtttagtca gctgcgaaac taaattcaag
     2461 tttacatact tagtgaaatt atgtttacca ggctgaaaca acaattgttt gttttcggta
     2521 ttctcgatta aattagcact tttcgataaa taattcgaaa tatccataat ttcttttgcg
     2581 atacgatttt gtctttcctt taacgcagga ttctttaacg caatatttct tgatgccagt
     2641 aaattatatt gtgcacttaa attcttacaa gaaagactat cggctttcat gatttaaaat
     2701 ttcctgtatt tgtatctgaa tcaaatattg aattcgcgat accttttgat ttacatagac
     2761 aaactcttca ctcgatactg catttttggt ttgattcaaa agagagctta aatcacttga
     2821 taatacttta taaaactgta tctcatccat gatgcatcaa attttgttcg ttttcctgat
     2881 atagatttgc ggtttgatgt aaatatgtag aaaaacgcgt taatgtttct aaatacgaca
     2941 tcatagcagg atttaatgtt tgaaactgtt ccataaaata tttagatgca ggagactgcc
     3001 aaatcgattc cacataatac atcttagatt caatttcatt aaatactgta cgaatctcgt
     3061 tggcatacgc atcgacttgt tttgaatacg ccattacatc ttcactagat atttgaattt
     3121 gtgtcataac cattcctcct acaataataa tttagcttgt tgaacacgat cttgttgaag
     3181 cttttcataa accgaagctg tttgttttag aacattacta tattctaaaa tcacttgata
     3241 catttcattt aggcttggtc taaattcttc taactgattt tgaaacgcca aatgatctac
     3301 accctgccaa atgctttgca tctgctccac tcttttatat aaattttgaa ttagatcatt
     3361 ataactatcc gctttgtttt tcacatagac acttgcttga tatacttctg aataatccac
     3421 tgaaattttc ataaataaat ttacctccca taaatcaaca tatcgcaatg cgcaatatga
     3481 ttttctttaa atgttttatt caaatccaaa aaacacatta ccgtaacatt aaacacatat
     3541 cgtacttcat actctaaaaa ctcatcaaaa aaatcttgta ctcgatacga ctcagaaaca
     3601 aaaatcatct ttcgatcaaa agaaagatat atgccgatct cattcataaa ctccaatccc
     3661 ttcattttga tctttaaaaa atacacaatc aaagctttca taaggaagtt tacgcctcaa
     3721 tgtatatcca tactccttca atccatagcc aacccacaat acttgcgcct gatataaagc
     3781 tttttgaaac acttcatttg tcaattgttt aaaatctaca caaataacat aaatatcact
     3841 atttgaacct aagtctgaac aaattttaag atgtgaaaga ttctcacata gcttataagc
     3901 tttacactta tattcttcat gcatatataa aatataaagc tttcttttct catcaataaa
     3961 cacagggcga tcactctgac tttcaaagcc aatacaattt cgttcttttc gataagaaaa
     4021 tagcttacac gctcttcttt ttaaattgga ttcaattact cgattataaa tacactctac
     4081 atatgtattt tcataacatg tcatgtttag ataagaaggc aattggtagg tgtcaaaata
     4141 tattctttta tcatccaaac tatccacatc aaatacatac ttactttgta tccatttcaa
     4201 tcgagtgttc tcattttccg taataataaa tgcacgcaca ttttcattca ataaagataa
     4261 atcaagattt tcatcttcaa aaacaataca aactccactt agaatctgat gaaaacacct
     4321 agatttaaaa aagttaaaat ccacataatc atttaataca tgagttccat atacataaat
     4381 ggtttcatcc ttaaagtaat ttattaagct atatacaata tccatactct ttgtatatat
     4441 aagctgtaaa tgatcaaaaa caagctcaca ctgtttttgt ttaaatggta aatccaaaat
     4501 tccaaagtcc tcttttgaaa ctttctttgg caacaatacc catgattttt cttctttcaa
     4561 cacattgatt ttttctttta atgcatctat tacacgctta cttaaatgtt gcttagattg
     4621 gataacttct tttcgctcat tgacttcacg ccacgacttt tcatccacat atttttgtag
     4681 ataaaaactt ttacattcca aactctcatt ttttgtttgt agaaccatat cccctggatt
     4741 ctttaaagta taagcctttt cgttttgtaa aatctcacga ctatcttgta cattattcac
     4801 atgaaagcac accttccaac ttgtatttga cataacttga tcatcaatga ttcccatagg
     4861 tttttgtgta cttagaacta aatgtatccc taaagacctt ccaatacgtg caatttcttg
     4921 aagctgtgac ataaattgcg gaaaacgagt ctttaattgt gcaaactcat ccacaaatat
     4981 ccatagatgc gaaagtgtgt gcgtttcatt ataggcattg atatcggcta cttttgccgc
     5041 taaaaataag cgttgccttt tttccagttc ataattcata ctcataaaaa agcgttccat
     5101 ggattgtgca tccagatttg taacaattcc tgcacaatgc gcaaattcat aaaatggctg
     5161 tccaaaagca cctcctttaa aatcgattaa tatatattga aattgacttg gagcattatg
     5221 ccaaatcatc atcatcaaca aaaaacttat aaattcactt tttccaaatc cggtcatacc
     5281 cgcaaccaat ccgtgagatc catatttacg aaaatccata tacacaattt gattggattc
     5341 acgcattcca acaggaactc tcatcacata gcgctctttt cgaatgggtc cattctcaat
     5401 caactgttga taaaaatcta tattctgtga ttgtaaaaga tatctttttt gatctttatt
     5461 tatattggaa atacatatat tttgaataca atagtcataa tggaaggatg gttctttctt
     5521 tcctatatag atcaaacaat aatactcttt cggctgacat tttaaatccc tagtaaccac
     5581 tatgaattct gaattcgact tttcattcac aatacaaaat ggattcaata aaacatcttt
     5641 tatttcatca aaatcttgca gccacaacca cttacgcatc gaatttgaat accacaaata
     5701 gttctcaaac aaaatcaaac tctcttctat attccctttg atccaacata cttccccttt
     5761 ttttaagaag acaggttgta gtattttgga atttaaagat tctatcaatc gctctctttt
     5821 ttgaattaaa tcatttaatg catattcata agacacttca cgatatgaaa tagcacacca
     5881 ctttaatttt gtgcacccca cataaacaat atccttcgtt tcatgtttaa aagtatccac
     5941 gtactttttg atatatagat ttaccttctt ttcaaaatca ttcttcaaca agccctcttt
     6001 ttcatacatt cgttttaaat atccatcata catatctaga cttttctgat tttcttttac
     6061 gctcaattta tattgatagt ttcgattata taaaccataa atcataaatg ttacagccat
     6121 tgttaaagag ctgatcatac tcatgaatag tgtttccacg ctttgttttt gaagataccc
     6181 aatcagaatc gaagaaataa aaccagaact tgcgatcata cacgatggcc caatggcact
     6241 aaaaagactc tgtttacaaa ctacacgatg gatttgtggc atctgtatgt cccacttttg
     6301 tattgttggt aattgcacat ttaaatttga tggatagaac atgggttctt ctcgttttaa
     6361 atccttattt tcaaaaggat tatccaccat atctacaagc agataatttt taaaataaat
     6421 catacgaaca ttcgcaatca caatttcatc ctgcatgttt aacttatatg aatgtacacg
     6481 cttagtgttc acatatgtac catttaaact atctaagtct gtcaatacat ccttttcaat
     6541 tttaaaatga aacc
//
LOCUS       DBMDWL010000117         6457 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4610_length_6457_cov_2.912996, whole genome shotgun sequence.
ACCESSION   DBMDWL010000117 DBMDWL010000000
VERSION     DBMDWL010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4610_length_6457_cov_2.912996"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(306..1097)
                     /locus_tag="ACZPDN_08255"
     CDS             complement(306..1097)
                     /locus_tag="ACZPDN_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015534.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR1298656.1"
                     /translation="MKLYIDENNKVVTMHEATSDMKCFEEFENSKPYTFDTVKEVKGL
                     ENSVHILLNTSMIDEKWIYMNLKKYISKQDRVCILPFSFFEDTKNEADWNKQYAPGQG
                     IWYRSNQDVFYKYGIGKDQIVWVNYFKDTIDEMKMKILNSSILMLTGGAPDLMMKRIK
                     EKKLKKIIKNYKGIMIGYSAGAMIQLDLYHISPDEDYPKFSYQNGLGCLSGFDIEPHF
                     RRSKIQMESIEKVKEEKGISIYGIYEDGGMIIDKGITCFGKIDLF"
     gene            complement(1131..1769)
                     /locus_tag="ACZPDN_08260"
     CDS             complement(1131..1769)
                     /locus_tag="ACZPDN_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298657.1"
                     /translation="MAKMKRKRVASNKSKGQGHKLVWFTLIIIMIPVVIVGYVLLTSI
                     GGQNHPVEGSRFSKTDLDPAITDDNISSLESALSNIENVDKVSVNLLSATLRVHIDLV
                     DSATDDVASAAIDSAYNIVNEQLPIDTYFTNKDGSKMYDLEIDSYNYLIDDTHTADGW
                     TYLKLTKTGASSGPITDNMTAAKDAELSNQLKAASEQIQQNNANAQNEEDGQ"
     gene            complement(1843..3264)
                     /gene="gatB"
                     /locus_tag="ACZPDN_08265"
     CDS             complement(1843..3264)
                     /gene="gatB"
                     /locus_tag="ACZPDN_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR1298658.1"
                     /translation="MNYFVTIGIEIHCELKTNTKMFSGAPVRFGETANTCTSVVDLGH
                     PGCLPCVNKEAVALAIKACTAMHCDLETLVRFDRKNYYYSDLPKGFQITQQFHPIGTN
                     GYVEIDVDGKKKQIRIERIHMEEDTAKQFHKETGTYIDFNRAGTPLIEIVSKPDMHSA
                     KEAAAYVETLRKNLLYLNVSDVKMEEGSMRCDVNISLSKDKDTLGTKTEIKNLNSIAN
                     VHKAVQAEIERQSALLDAGEKVEQATRRYDEAQKTTILMRKKEGNVDYKYFPEPNIFP
                     IQLDLDWVKSIQDNLEELPDARLARFMKDYELGEYDASVLVSDREMADFFEEVLKSTK
                     FAKKACNWVIGDVSAYLNKNNLSFKNIPCSSENLASLINVIEAGEISGKQGKVVFEEV
                     MQGKDPKKVIEEKGMKQVSDDGAILAIVNSVLDANPQSIEDFKNGKDRAVGFLVGQVM
                     KASRGQANPKRTNELIQEELKKR"
     gene            complement(3267..4640)
                     /locus_tag="ACZPDN_08270"
     CDS             complement(3267..4640)
                     /locus_tag="ACZPDN_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR1298659.1"
                     /translation="MEISNCVNKNVAIEKINAVQPKLNAVVTMIDEDVNTNAGKLAGV
                     PVALKDNISTCGIKTTASSRILDNYVPVYDATIVKKLREAGADFVCKASMDELGMGGT
                     NKNAYTGAVHNPYDLDRIPGGSSGGCAALVAAGCVPLAIGTDTGDSVRKPAAYCGVVG
                     VKPTYGRISRYGIIPYASSLDHVGYFTTNVKDAALALEVLAGRDDHDMTSSYKEVGEY
                     SKLLNSDLTNKTIGVFKTVFDEVENEELKAQFNALVSKLEEKGAKIVNVEMPKEYMEL
                     IAPVYKTIANAEATANHSNLDGIRFGMREEGQDLEEVMTNTRTKGFSSYVRARFVIGS
                     YSLFEENQDRLFRKAQKIRRLIVQAYAKMFEECDAVLSVAAPTIAPLIHESSNVRFGV
                     SSFAEEHMQLQNFSGYPSITLPLGKVDSMPVAVNLTCKPFEEQKMFDVSSAIEECTGL
                     KGQCVEG"
     gene            complement(4642..4935)
                     /gene="gatC"
                     /locus_tag="ACZPDN_08275"
     CDS             complement(4642..4935)
                     /gene="gatC"
                     /locus_tag="ACZPDN_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR1298660.1"
                     /translation="MEKFSTEYFHKLANDLKFDLSDEEIEELKKDFVAVEKQVSLFEK
                     VDTQGVEPMVYPFETPTVFLREDVVSDVLTQEEALKNVKDARMGHVHVPKVVK"
     gene            complement(4945..6015)
                     /locus_tag="ACZPDN_08280"
     CDS             complement(4945..6015)
                     /locus_tag="ACZPDN_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HIR1298661.1"
                     /translation="MRKSKLLIGLLAMSLCACSTGNKNQANALDEDSYQAILPYEASD
                     TRSKHVGLINDTDLRVEMESGLMDLSKKYFSTSSVGYKTHQFLDYDELDATDGSRGLL
                     GTVRDGNPNGLNPSADEEFDTGNGIVTNATILVDIYELDWYTNDTLKGISLGLVVNGN
                     LNASDGSSVDITDEKMQNYLEVTFSKLASYMHERFNEINKNIPIYIAAYKLDDTNTTD
                     KGGYVYEGYYKGGQGNFTSLTQEWVLVPSSRFTELDATAADEFTTFKEEIANVLPDNT
                     FVTGEAKFESKKLRKLNLTITAHGKTAGEVLAIIEYAKDQMSTFETKKCDYLITILND
                     DTVYALIERKSGSSECNMISKI"
     gene            complement(6002..>6457)
                     /locus_tag="ACZPDN_08285"
     CDS             complement(6002..>6457)
                     /locus_tag="ACZPDN_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1298662.1"
                     /translation="GKKAAMILAEQFGDIDTLMHACVDELVLIHDIGLITASSVVAFF
                     KEEKNLHLIDVLKQAGCNFKQEKKQVVESRFTNKTCVLTGTLEHYTRNEAKAILESLG
                     ANVSGSVSKKTDYVIYGTAAGSKLTKAQSLGVMTMSEEEFVQEVENAKE"
BASE COUNT         1952 a         1390 c          840 g         2275 t
ORIGIN      
        1 attactgtaa tttgtaacct catcattgac cttcatattc tgacaaagca tggccggtgt
       61 tttataatac acctgtccat tttcttcaat acgatcaatg ggatatccat tatttaaaag
      121 ccactccaac aattctagat ccacttctgt gatcaactta caaaatttca tctacgctct
      181 cctatttcta accatttcac atgtgactca ctatattaag tatcccatat taatgtgaat
      241 ttaacatgaa aaaataacta aaaactaaat tgtgcaaatt ttagattaat ttgtgcaaat
      301 aataatcaaa ataaatcgat ttttccaaaa caagtaatac ccttatcgat gatcatgcca
      361 ccatcttcat agattccata tatggaaatt cctttttctt ctttgacttt ttcaatactt
      421 tccatttgaa tcttacttct tctaaaatgg ggttcaatat caaaaccgga taaacaacct
      481 aaaccatttt gatacgaaaa ttttggataa tcttcatccg gacttatatg atacaaatcc
      541 agttggatca tagctccagc actatatcca atcataatac ccttataatt tttaatgatt
      601 ttcttcagct ttttttcttt gatgcgcttc atcatcaaat ctggagctcc acctgtcaac
      661 attaaaatgg aactatttaa tattttcatc ttcatttcat cgatcgtatc tttaaaataa
      721 ttcacccata caatttgatc tttgccaata ccatatttgt aaaatacatc ttgattcgaa
      781 cgataccata tgccttgtcc tggtgcatat tgtttattcc agtcagcctc attttttgta
      841 tcctcaaaaa agctaaatgg taatatacaa acacgatcct gttttgaaat atactttttt
      901 aaattcatat agatccattt ttcatcgatc atacttgtat tgagcaatat atggactgaa
      961 ttttctaaac cctttacttc ttttactgta tcaaacgtat agggtttact attttcaaac
     1021 tcttcaaaac acttcatatc actcgttgcc tcatgcatag tgactacttt attattctca
     1081 tcaatatata atttcatttt tgttttacct ccaagaaaaa agatggacgc ttattgtcca
     1141 tcctcttcat tttgtgcatt tgcgttattc tgttgaattt gttcacttgc tgcttttaat
     1201 tgatttgaaa gctctgcatc tttagcggct gtcatattgt ctgttatagg accactactt
     1261 gcacctgtct ttgtcaattt taaatatgtc caaccatccg cagtatgtgt atcatcaatt
     1321 aaataattat aagaatcaat ttctaaatca tacatttttg atccatcttt gtttgtaaaa
     1381 tatgtatcaa ttggaagttg ctcattcaca atattataag cactatcaat agctgcactt
     1441 gccacatcat ccgtagcact atctaccaaa tcaatatgta ctctaagtgt agcactcaat
     1501 agattcacac ttactttatc tacattttca atattggaaa gtgcactctc taatgatgaa
     1561 atattatcat ccgtaatagc tgggtctaaa tctgttttag aaaaacggct tccttcaaca
     1621 ggatgatttt gcccacctat actcgtcaat aatacataac ccacaatcac aaccggaatc
     1681 ataattataa ttaacgtaaa ccatacaagt ttatgtcctt gtcctttaga tttattggaa
     1741 gcaactcttt tacgtttcat cttggccata aaaaacctcc taacaacatt cttattatac
     1801 cacaggatat aaaaacatta aaagtctgaa tgaatcagac ttttatcttt tctttaactc
     1861 ttcttgaatc aattcgtttg tacgcttagg atttgcctgt ccacgacttg ccttcatgac
     1921 ctgtccaacc aagaatccta cagcacgatc cttaccattt ttaaaatctt caatagattg
     1981 agggttggca tctaaaacac tattgacgat tgccaaaata gcaccatcat cactcacttg
     2041 tttcattccc ttttcttcga tgaccttctt aggatctttt ccttgcataa cttcttcaaa
     2101 aacgacttta ccttgtttac cactaatttc accagcctca ataacattga tcaaactagc
     2161 taagttttca cttgaacaag gaatattttt gaagcttaaa ttatttttat ttaaataagc
     2221 tgaaacatcc ccaatcaccc agttacatgc ttttttcgca aattttgtag atttcaatac
     2281 ttcttcaaag aaatccgcca tttcacgatc tgaaaccaat acactcgcat catattcacc
     2341 aagttcataa tctttcatga aacgtgctaa acgtgcatct ggaagctctt ctaagttatc
     2401 ttgaatggat ttaacccagt caagatctaa ttggattggg aaaatatttg gttctgggaa
     2461 atatttatag tcaacatttc cttctttttt acgcattaga attgtagttt tttgtgcttc
     2521 atcataacga cgagtcgcct gttctacttt ttcacctgca tctaacaagg cactttgacg
     2581 ttcaatttca gcttgaactg ccttatgtac attcgcaatc gagttcaagt tcttgatttc
     2641 tgtttttgtt cctaaagtat ctttatcttt actcaacgaa atattgacat cacaacgcat
     2701 acttccctct tccatcttaa cgtcactgac attcaaatat agaaggtttt tacgtaaggt
     2761 ttctacataa gccgctgctt cttttgcgct atgcatatct ggcttagata caatttcaat
     2821 caatggcgta cccgcacggt tgaaatcaat ataagtacct gtctctttat ggaactgttt
     2881 tgcggtatcc tcttccatat gaatacgttc gatacgaatt tgtttctttt tgccatctac
     2941 atcaatttct acataaccat ttgttccgat tggatggaat tgttgtgtaa tttgaaatcc
     3001 cttaggaaga tctgaataat aatagttttt acggtcaaaa cgaaccaatg tttctagatc
     3061 acaatgcata gctgtacatg ccttaatagc taaagctacg gcctctttat ttacacaagg
     3121 taaacatcca ggatgtccta aatcaacaac gcttgtacat gtatttgccg tttctccaaa
     3181 acgaacggga gcacctgaaa acatttttgt gtttgtcttt aattcacagt gaatttcaat
     3241 accgattgtt acaaaataat tcattcctat ccctccacac attgtccttt taaaccagtg
     3301 cactcttcaa tagcacttga cacatcaaac attttttgtt cttcaaatgg cttacaagtt
     3361 aaattcacag ctacaggcat agaatcaact ttacctaaag gaagtgtaat acttggataa
     3421 ccagagaaat tttgtagttg catatgctct tcggcaaaac tagatacacc aaaacgaaca
     3481 tttgaacttt catggatcaa tggtgcaatt gttggtgctg caacacttaa tacagcatca
     3541 cattcttcaa acatcttcgc ataagcttga acaattaaac gacgaatctt ttgtgctttt
     3601 ctaaataaac gatcttggtt ttcttcaaat aaagaataag acccaatcac aaaacgagca
     3661 cgaacataac ttgagaaccc ttttgtacgt gtatttgtca tgacctcttc taaatcttga
     3721 ccttcttcac gcataccaaa acgaatacca tctaaatttg aatggttggc cgttgcttct
     3781 gcattcgcaa ttgttttata aacaggggca atcaattcca tgtattcttt cggcatttca
     3841 acattgacaa tttttgcccc tttttcttct aatttagaaa ctaaggcatt aaattgcgct
     3901 tttaactctt cattttcaac ttcatcgaaa acagttttaa atacaccaat tgttttattt
     3961 gtaagatcac tattcaataa tttcgaatat tcaccaactt ctttgtagct tgaagtcata
     4021 tcatggtcat cacgacctgc caaaacttct aaggctaaag ctgcatcttt tacatttgtc
     4081 gtaaaatatc caacgtgatc caaagaactt gcataaggaa taattccata acgtgaaata
     4141 cgtccataag ttggttttac accaacaaca ccacaatatg ctgcaggttt tcgaacacta
     4201 tcacctgtat ctgtaccgat tgccaaaggt acacatcctg cggcaactaa agcagcacat
     4261 cctccactag atcctcctgg aatacggtct agatcgtaag gattgtgtac agctcctgta
     4321 taggcatttt tattggttcc acccatacca agctcatcca tacttgcttt acatacaaaa
     4381 tcagcaccag cttctcttaa tttctttacg attgtcgcat cataaacagg aacatagtta
     4441 tctaaaatac gactgcttgc cgtagtttta ataccacaag tagaaatgtt atctttcaat
     4501 gcgactggaa caccagcaag ctttcctgca tttgtgttca catcttcatc gatcatagtt
     4561 acaaccgcat ttaactttgg ttgtacggca ttgatttttt caatagccac atttttattt
     4621 acacaatttg aaatttccat cttacttcac cacctttgga acgtgaacat gtcccatacg
     4681 agcatcttta acatttttta atgcttcttc ttgcgttaat acgtcactta caacatcttc
     4741 tcttaagaag actgtaggtg tttcaaaagg atagaccata ggttctaccc cttgggtgtc
     4801 cactttttca aaaagggata cttgcttctc gacggcaaca aaatcttttt tcaattcttc
     4861 gatttcttcg tcactcaaat caaactttaa gtcgtttgct aatttgtgaa aatattctgt
     4921 actgaacttt tccatattct cctcctatat cttactgatc atattacatt cacttgaacc
     4981 agattttctt tctattaatg catacactgt atcatcattt agaatggtaa tcaaatagtc
     5041 acacttcttc gtttcaaatg tactcatttg atctttcgca tattcaatga tagctaaaac
     5101 ttctccagct gtttttccgt gtgctgtaat tgtcaaattt aattttctta gttttttcga
     5161 ttcgaacttc gcttctcccg tcacaaacgt gttgtctggt aaaacgttcg ctatctcttc
     5221 tttaaaggtt gtaaattcat ccgcggccgt tgcatccaat tctgtaaagc gagagcttgg
     5281 aactaagacc cattcttgtg ttaaagatgt aaaatttcct tgtccaccct tataataacc
     5341 ttcatataca tagccaccct tatctgtcgt atttgtatcg tccaatttat aggccgctat
     5401 atagatagga atatttttat taatttcatt aaaacgctca tgcatataag atgctaattt
     5461 agaaaaagtc acttccaaat aattttgcat cttttcatct gtaatatcta cactcgatcc
     5521 atcggatgca tttaagtttc cattgacgac taaacccaat gaaatacctt ttaatgtatc
     5581 gttggtatac cagtctaatt cataaatatc cactagaatt gtcgcatttg taacaattcc
     5641 atttcccgta tcaaattctt catctgcact tggatttaga ccattgggat tgccatcacg
     5701 aaccgttcca agtaatcctc tagatccgtc tgtcgcatct aattcgtcat aatctaagaa
     5761 ttggtgtgtc ttataaccta cagaagaagt ggaaaaatat ttcttcgaaa gatccatcaa
     5821 tccactttcc atttcaacac gcaaatctgt atcgttgatc aaaccaacat gtttacttcg
     5881 tgtatcactt gcttcataag gcaaaatagc ctgatacgaa tcttcatcta aggcattcgc
     5941 ctgattttta ttaccggtag aacaagcgca taaactcatt gctaataaac caatcaataa
     6001 tttactcttt cgcattttct acctcctgta caaattcttc ttcactcatt gtcataacac
     6061 ctaagctttg tgcctttgta agcttagatc ctgcagccgt accataaata acataatccg
     6121 tctttttgga tacagagcca gaaacattcg ctcctaatga ttctaaaata gcttttgcct
     6181 cattacgcgt ataatgttct aatgtacccg ttaaaacaca tgttttatta gtgaatcgac
     6241 tttcaacgac ttgtttcttt tcttgcttga aattacatcc agcctgtttt aatacatcaa
     6301 ttaaatgaag attcttttct tctttaaaga atgccacaac acttgaagct gtaatcaagc
     6361 caatatcatg aatcaaaact aattcatcaa cacaagcatg cattaaagta tcaatatcac
     6421 caaattgctc agctaagatc attgcggctt ttttacc
//
LOCUS       DBMDWL010000118         6429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4633_length_6429_cov_3.102876, whole genome shotgun sequence.
ACCESSION   DBMDWL010000118 DBMDWL010000000
VERSION     DBMDWL010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_6429_cov_3.102876"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            397..2109
                     /locus_tag="ACZPDN_08290"
     CDS             397..2109
                     /locus_tag="ACZPDN_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1298663.1"
                     /translation="MNKQRIVAGLASVAMASTLVACSGGSNSDAKTLTVSVNQSLTGQ
                     FTPQYASSAYDQYVVNLCYESMLKYNADNELEPELAKDLPEVSADGLTLTYKLEKGHK
                     FSDGTEVTSKDVKFTFATLADPSYAGPNGGGLENLVGYKEYNSGAAKELTGIETPDDY
                     TVVFHLVSPQIDAIAKFGGMGITSADHYSKYKQGKVKVVEKNQEESCGSGAYQIKSYD
                     KASGASFVRNEEFKREKGQYQVDQIIIKKTDVTTEVSELKKGTVDLIPDVIETNKVSE
                     ASQDKNMAFNYYSRSAVGFVAMNANNGATADKAVRQALMYATDRQGFCDSYFGWDKKA
                     SDEVKKVKGGYVPAAYWNPASVNCGAVVRNEETIDGLNTYAFDMDKANQVLDEAGWVR
                     GADGIREKDGQKLEIKFLLSEGNSVLETLIPMVKKTWSDLGVDLKQTTVDFSTLLSNI
                     QDESHDSEWNMCFLATSFTDNQDAGANDSYTNNPNNMAVNNYSRINDQALVDQLTKAR
                     AESDAEKSKAEYVEAMKMAADDAGYMPVYSGMQFNLYNKKVDLTGTGTLRNWSQSMDT
                     ITVK"
     gene            2291..3274
                     /locus_tag="ACZPDN_08295"
     CDS             2291..3274
                     /locus_tag="ACZPDN_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1298664.1"
                     /translation="MLKYVFKRILAMIPVLFIISIVIFGTVKAMPGDEVTAYFGAGSK
                     VTQEQRDHVRELLGLDKSLPEQYVRWVGRMCTGDLGSSITLKKPVGTIIGDYVWNTFY
                     LNVVSLIVALIFAIPVGIKQAVKKGSVFDNFWTVFSLLGISVPTFFFALVLIFFIGLN
                     IPGMPINGMRDTMLSSFGYSSIFQEIGDIALHSILPVIVLAFSSFASFSRYVRNSMVE
                     VINMDYVRTARSKGLKEKTVIYKHAFKNAQIPLVTLLGLYIPSLFSGAVILESVFIWP
                     GIGKVLIDAINQRDNSLVMACLMFSALLMLLGNLLSDICYTLVDPRIKVDE"
     gene            3288..4199
                     /gene="opp4C"
                     /locus_tag="ACZPDN_08300"
     CDS             3288..4199
                     /gene="opp4C"
                     /locus_tag="ACZPDN_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HIR1298665.1"
                     /translation="MAKKDAVVLESGEKIESIGPWTIAWRKFRQNRVAMAGLIIFILI
                     VIAVIVIPPMLGYSVNDYDLSNPNLAPGGEHLLGTDKQGRDCLFRLFLGGRISIMVGI
                     IAAVITVILGCTIGGISGYYGGKLDNVLMRFAEIVYSLPFTPMIIAVASTMLWVPQSQ
                     KMYTVSLLIGLLSWPGLARLVRGQILTLREQEFMQACEALGISDFSKIFKHLLPNVIS
                     LVIVNATLQMASAILTEAGLSFLGMGVTAPTPSWGNLMNLARDSYVFQNYPWEWIPAG
                     LMCLLTVISINLIGEGLRDAFDPKELQ"
     gene            4215..5234
                     /locus_tag="ACZPDN_08305"
     CDS             4215..5234
                     /locus_tag="ACZPDN_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298666.1"
                     /translation="MSVNKKVIKVEDLRVYFHTKKGDSKAVDGNHFTMYENETLAIVG
                     ESGCGKSVTAMSILGLVSEPGEIMPGSKIVYMGNVDRDEDGLNLATATDEQIRHIRGN
                     EISMIFQEPMSSLNPVITVGDQIMENILTHEEVSKEEAKQRAVELLSEVGISRPEKVV
                     DNYPFQLSGGMCQRVMIAMALSCHPKLLIADEPTTALDVTIQAQILSLMNKLKNETGT
                     SIMLITHDLGVVAQVADNVNVMYAGKVVETAPVEELFSNPKHPYTVGLMNSMPSLAEE
                     GKRLNTIEGSVPNPLYLPKGCYFADRCPYVTDRCKEGQPTDTKIKSRHHVWCFKVEEE
                     MKQEG"
     gene            5237..6220
                     /locus_tag="ACZPDN_08310"
     CDS             5237..6220
                     /locus_tag="ACZPDN_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298667.1"
                     /translation="MAEKRKILEVKNLKKYFPLKKGKYKEGDPCVKAVDDITFDLYEG
                     ETLGLVGESGCGKSTLGRTIIRLYEPTSGEVIFEGEDVAKKSRKEMRALREEMQFIFQ
                     DPYSSLNPRMTVFNILAEPLIAHGKFKRGPELDAYVKNLMDRCGLPSYYCYRYPHQFS
                     GGQRQRIGIARSLALDPSFIICDEPVSALDVSIQSQIINLMKDMQEEKNISYIFISHD
                     LSVVKHISDRVGVMYLGSMMELADKNEIYSNPQHPYTRALIGAIPLPDPTKRKEMKVI
                     QGEIPSNVNIPKGCKFNPRCPFAKDICREQEPATKEVKPNHFVKCHFGGEF"
     gene            6220..6411
                     /locus_tag="ACZPDN_08315"
     CDS             6220..6411
                     /locus_tag="ACZPDN_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298668.1"
                     /translation="MSKRPSMREMLEKKDKQKGKEDRLEKGDFLALFIAASSTFWPIL
                     LGAIVILALLIFGWNAIFA"
BASE COUNT         2168 a          988 c         1278 g         1995 t
ORIGIN      
        1 tgtcacaatt ttattttaac tttggtaaaa gttgtgaaat tgtaaagtaa accattttca
       61 agaattagag tagttttctt tatgtaatat gaattataaa ttgtagattc atattttaaa
      121 agtgatatat agataaaaat ttgaattgta ttttcatggt ttacataaaa atctagtgtg
      181 tatagacaaa tgtttgtaaa tgaaatacga aaaaccaaat gaaagcgaat acaatgaaaa
      241 tacttgatat aaagacaaaa tgatgtgttg tgttcatgaa aaatatacat taatgtaaaa
      301 ttgtatgtaa atatttttct taaacccttg aaaacgtgtt tctaacatag tataatttca
      361 tcacacatag tgagcagact ataggaggaa agagaaatga ataaacaaag aattgttgct
      421 ggtttagcat ctgtagcaat ggcttctact ttagtagcat gttcaggcgg atctaacagt
      481 gacgcaaaaa cattaacagt atctgtaaac caaagtttaa ctggacagtt tacacctcaa
      541 tatgcatcaa gtgcttatga tcaatatgta gtaaacttat gctacgaatc aatgctaaag
      601 tacaatgctg ataacgaatt ggagccagaa ttggctaaag atttaccaga agtaagtgca
      661 gatggtttaa cattaactta caaattggaa aaaggacaca aattcagtga tggaactgaa
      721 gtaacttcaa aagacgttaa atttacattc gcaacattag ctgacccaag ctatgctggt
      781 cctaatggtg gtggattgga aaacctagta ggatataaag aatataatag cggagctgct
      841 aaagaattaa ctggtattga aactccagat gattacacag tagtattcca cttagtatca
      901 cctcaaatcg atgcaattgc aaaatttggt ggaatgggaa tcactagtgc agatcactac
      961 tcaaaataca aacaaggtaa agtaaaagtt gttgaaaaga accaagaaga atcatgtggt
     1021 tctggtgctt accaaataaa gagctatgat aaagcaagtg gagcttcatt tgttcgtaat
     1081 gaagaattca aacgtgaaaa gggacaatac caagtagatc aaatcattat taagaaaaca
     1141 gatgtaacta cagaagtttc tgaattgaaa aaaggaacag ttgatttaat tccagatgta
     1201 attgaaacaa ataaagtaag tgaagctagc caagataaaa acatggcatt caactactac
     1261 tctcgtagtg ctgttggttt cgtagctatg aacgctaaca atggagctac agctgacaaa
     1321 gctgtacgtc aagcattgat gtatgcgaca gatcgtcaag gattctgtga ttcttacttc
     1381 ggatgggata aaaaagcaag tgacgaagtt aaaaaagtta aaggtggata tgtaccagct
     1441 gcatactgga acccagcatc agtaaactgt ggtgccgttg ttcgtaatga agaaacaatc
     1501 gatggattaa atacatatgc atttgatatg gataaagcga accaagtatt agatgaagct
     1561 ggttgggttc gtggtgctga tggaatccgt gaaaaagatg gacaaaaatt agaaattaaa
     1621 ttcttattgt cagaaggtaa ctcagtactt gaaactttaa ttcctatggt taagaaaact
     1681 tggagcgatt taggtgttga cttaaaacaa actacagttg acttctctac attattaagc
     1741 aacattcaag atgaaagcca cgatagcgaa tggaatatgt gtttcttagc tactagcttt
     1801 acagataacc aagatgctgg agctaatgat tcatatacta ataatccaaa taacatggct
     1861 gtaaataact attctcgtat caatgatcaa gcattggttg accaattgac aaaagctcgt
     1921 gctgagtcag atgcagaaaa atcaaaagca gaatatgttg aagctatgaa gatggctgct
     1981 gatgatgcag gttacatgcc agtttactca ggtatgcaat tcaaccttta caataagaaa
     2041 gttgacttga ctggaacagg aacattacgt aactggtctc aatcaatgga tacaattact
     2101 gtaaaataaa tttaaaaatt gcacaaagat gtgttaaaat agtggatgtt aaaggacaaa
     2161 tgttcattaa cagaaacgct gcaacgttaa atagtctgca tgaaaaattc gtggattgtt
     2221 tataaaatta agggcaagcc gtattgactt gctcttatat gcatttagtg gatagaaagg
     2281 atcgataact atgctaaagt atgtatttaa aagaatttta gcgatgattc ctgtcctttt
     2341 catcatttca atcgttattt tcggtactgt aaaggcgatg ccaggtgatg aggtaacagc
     2401 ttattttgga gccggttcaa aggttacaca agagcaacgt gatcacgttc gtgagttgtt
     2461 aggattggat aaatcgttac ctgaacaata tgtacgttgg gttggacgta tgtgtacagg
     2521 agatttagga tcaagtatta ctcttaaaaa accagttgga actattattg gtgactatgt
     2581 atggaataca ttctatttga atgttgtttc tttaatagtg gctttaattt ttgcaattcc
     2641 agttggaata aagcaagccg taaagaaagg ttcagtgttt gataacttct ggacagtatt
     2701 ttcattgtta ggaataagtg ttccaacatt cttctttgcc ttagtattaa ttttcttcat
     2761 tggtttgaac atcccaggca tgccaatcaa tggaatgcgt gacacaatgc tttcaagctt
     2821 tggttactca agtattttcc aagaaattgg agatatcgca ttacactcaa ttttacctgt
     2881 aatcgtattg gcgtttagtt catttgcgtc attctcacgt tatgtacgta actctatggt
     2941 tgaagtcatc aacatggatt atgttcgtac tgcgcgttca aagggtctaa aagaaaagac
     3001 tgttatttac aaacatgcat ttaaaaatgc tcaaattcct ttagtaacat tactaggatt
     3061 atacattcca tcattattct caggagccgt aattttggaa tctgtattta tctggccagg
     3121 aattggtaaa gtattaattg atgcgatcaa ccaacgtgat aactctttag ttatggcttg
     3181 tttgatgttt agtgccttgt tgatgttgtt aggtaactta ttgtctgaca tttgttatac
     3241 attagttgac ccacgtatca aggttgatga ataggaggga actatagatg gctaaaaaag
     3301 atgctgtagt attagaatct ggtgaaaaaa ttgaatctat aggtccttgg accatcgcgt
     3361 ggcgtaagtt tagacaaaac cgtgttgcca tggcaggttt gattattttt attttaattg
     3421 ttattgctgt tattgttata ccaccaatgc ttggatatag cgtaaatgac tatgatttat
     3481 caaatcctaa cttagctcca ggaggagaac atttattagg aacagataaa caaggtcgtg
     3541 actgcttatt ccgtttattc ttaggtggac gtatctctat tatggttggt attattgctg
     3601 ctgttatcac tgtcatctta ggatgtacaa ttggtggtat ctcaggatat tatggtggaa
     3661 aattagataa cgtattaatg cgttttgcag aaatcgttta ttcattacca tttacaccaa
     3721 tgattattgc ggttgcctca actatgttgt gggttccaca aagtcaaaaa atgtatactg
     3781 tatcattatt gattggttta ttatcatggc caggacttgc tcgtttagtt cgtggacaaa
     3841 ttttaacttt acgtgaacaa gaatttatgc aagcttgtga agctttaggt atttcagatt
     3901 tctcaaaaat ctttaaacac ttacttccaa acgttatttc tttggtaatc gtaaatgcta
     3961 cattacaaat ggcgagtgct atcttgactg aggctggttt atcattctta ggtatgggtg
     4021 ttacggcacc aactccttct tggggtaact taatgaactt ggcacgtgat tcttacgtgt
     4081 tccaaaacta tccttgggaa tggatccctg caggtttaat gtgtttatta actgtaatct
     4141 ctatcaactt aattggtgaa ggtttacgtg atgcattcga ccctaaagaa ttacagtagt
     4201 aaggaggata cacaatgtct gtaaataaaa aagtaattaa agtggaagac cttcgtgtat
     4261 acttccatac aaaaaaaggt gattcaaaag cagttgatgg aaaccacttt acaatgtatg
     4321 aaaatgaaac acttgcgatc gtaggtgaat caggttgtgg taaatctgta actgccatgt
     4381 ctattttagg cttagtcagt gaaccaggag aaattatgcc tggatcaaaa atcgtatata
     4441 tgggaaatgt agatcgtgat gaagatggtt tgaacttagc tacagctaca gatgaacaaa
     4501 ttcgtcacat tcgtggtaac gaaatttcta tgattttcca ggaacctatg tcaagtttga
     4561 acccagttat tacagttgga gatcaaatca tggaaaacat tttgacacac gaagaagttt
     4621 ctaaagaaga agctaaacaa cgtgcagttg aattgttgtc agaagttggt atttctcgtc
     4681 cagaaaaagt tgttgataac tatccattcc aattatcggg tggtatgtgt cagcgtgtta
     4741 tgattgcgat ggctttatct tgtcatccaa aattattgat tgccgatgaa cctacaacag
     4801 ctctagacgt tacaattcag gcccagattc tatctttaat gaacaaatta aagaatgaaa
     4861 caggtacatc tattatgttg attacacatg acttaggtgt agttgctcag gttgccgata
     4921 acgttaatgt tatgtatgct ggtaaagttg ttgaaacagc tccagtagaa gaattgttca
     4981 gcaatcctaa acacccatat acagttggat taatgaattc aatgccttca cttgcagaag
     5041 aaggaaaacg attaaataca atcgaaggta gcgtacctaa cccattgtat ttaccaaaag
     5101 gatgctactt tgcagatcgt tgtccatatg taacagatcg ttgtaaagaa ggtcaaccaa
     5161 cagatacaaa aattaaatca cgtcaccacg tatggtgctt caaagttgaa gaagaaatga
     5221 aacaggaggg atagtcatgg cagaaaaaag aaaaattctt gaagttaaaa atcttaaaaa
     5281 atacttccct ctaaaaaaag gaaaatataa agaaggcgac ccatgtgtta aggccgtaga
     5341 tgatattact ttcgatttat atgaaggaga aacattaggt ctagtaggtg aatcaggatg
     5401 tggtaaatct actttaggac gtacaatcat tcgtctttat gaaccaacaa gcggagaggt
     5461 aatcttcgaa ggtgaagatg ttgcgaaaaa gtctcgtaaa gaaatgcgtg ctttacgtga
     5521 agaaatgcag tttatcttcc aggatccata ttcttcattg aatccacgta tgactgtatt
     5581 caatattttg gcagaaccat tgattgctca cggaaaattc aaacgtggtc cagaactaga
     5641 tgcatatgtt aaaaacctaa tggaccgttg tggtttgcca agttattact gctatcgtta
     5701 tcctcaccaa ttctcaggtg gacaaagaca acgtattggt atcgcacgtt ctttggcatt
     5761 agatccaagt ttcattattt gtgatgaacc agtaagtgca ttggacgttt ctatccagtc
     5821 acagatcatc aacttaatga aagatatgca agaagaaaag aacatttctt atatcttcat
     5881 ttcacatgac ttgtctgttg taaaacacat ttctgaccgt gttggtgtaa tgtacttagg
     5941 tagtatgatg gaacttgcag ataagaatga aatctattca aaccctcaac acccttatac
     6001 aagagcgttg attggtgcaa ttcctttacc tgaccctaca aaacgtaaag aaatgaaagt
     6061 tattcaaggt gaaattccaa gtaacgtaaa cattcctaag ggatgtaaat tcaaccctcg
     6121 ttgtccattt gcgaaagaca tttgtcgtga acaagaacca gcaacaaaag aggttaagcc
     6181 aaatcacttc gttaaatgtc actttggagg tgaattctaa tgtctaagcg cccatctatg
     6241 cgtgagatgt tagaaaaaaa agataaacaa aaaggtaaag aagatcgtct tgaaaaaggg
     6301 gattttctag ccttattcat tgctgctagt agtacattct ggccaatttt attaggtgcg
     6361 attgttattt tagcactatt aatttttggt tggaatgcca tctttgcata atacgagaag
     6421 ctttcggct
//
LOCUS       DBMDWL010000119         6429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4635_length_6429_cov_2.512326, whole genome shotgun sequence.
ACCESSION   DBMDWL010000119 DBMDWL010000000
VERSION     DBMDWL010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4635_length_6429_cov_2.512326"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            21..791
                     /locus_tag="ACZPDN_08320"
     CDS             21..791
                     /locus_tag="ACZPDN_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298669.1"
                     /translation="MFNSNGVSLADIAAVTGNNRNNDGMWGNGAWWIVILLIFGWGNN
                     GWGGFGGNGNGAGYTDSAIQRGFDNQAVISKLDGISNGLCDGFYAMNNSMLTGFNGIN
                     TNIMQTGYGIQQAINADTVANMQNTNALQAQLANCCCETREAIQGVNYNMATNTCALQ
                     NTMNNNTRDIIDSQQAGTRAILDYLCTKENADLRDKVQKLELSASQDRQNALLTTAMT
                     AQTQQIVNSVNPTAIPAYVVPNPNAYAYGYGCNTGCNC"
     gene            914..1333
                     /locus_tag="ACZPDN_08325"
     CDS             914..1333
                     /locus_tag="ACZPDN_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298670.1"
                     /translation="MEITGIALQTVAAGEDVAFTETPVCGTKCIVHRQGSGIIKLRGI
                     TNQCKARFLVSYSGNIQIPTGGTVGAISLAIAVDGEPLQSTRMVVTPAAVENLFNVSA
                     QAYVDVPCGCCSTVAVQNTSTQAVEVQNSNLIAVREA"
     gene            1338..1976
                     /locus_tag="ACZPDN_08330"
     CDS             1338..1976
                     /locus_tag="ACZPDN_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298671.1"
                     /translation="MHIERIHKMVECLTEKTLSELDKGIENVNVEEMSEAVDMIKDLC
                     EAEYRAVIVKSMKKADEEEEEYDKELLRSLKTEYGEENGRRFYDHYRYANGRFAPKGK
                     GTYRRGYEEPPYMHMYPEAEHMRDMDRDYGKMYYTEPMNESGYDRAKRNYTETKEMHS
                     GTTPEDKEQRMKALDKYIKTVTNEIIKIVSDNATTEEKSLIKNNMNNLMSKF"
     gene            2036..2356
                     /locus_tag="ACZPDN_08335"
     CDS             2036..2356
                     /locus_tag="ACZPDN_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR1298672.1"
                     /translation="MIFNINGTMWRVQYENSNSSELKRSDNVSVLGVTDRNTHTIYLS
                     NALRGFMQRKVLIHEVCHAICMSYDVYLPIEQEEILCDFVATYGDEVFDIVDMVLGAA
                     RRVG"
     gene            2634..3332
                     /locus_tag="ACZPDN_08340"
     CDS             2634..3332
                     /locus_tag="ACZPDN_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298673.1"
                     /translation="MRVVRLKMYQEMARFNNPSAPKGADCYPLPPFSTVNGFIHSMCQ
                     WKRYHKLDYFVTGKGIYNTKVQKEWHGGYNFNKISDEMLKRWDVITDYADGSHTGWVS
                     TVKYHLMLVDLYTTIYIKADDSDIDDIYHALLNPPVYPSLGEYGDLCKIEAVDIVELK
                     ELDKCVPAPLAMQSYIPVNKGNFAGTIYRINNKYEIIEGLRRFQKVSCYLVDKGQEVV
                     SNLFDDDKPIIFIN"
     gene            complement(3756..4259)
                     /locus_tag="ACZPDN_08345"
     CDS             complement(3756..4259)
                     /locus_tag="ACZPDN_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298674.1"
                     /translation="MHFLRFGEIPKNEKSINFLKMTNDQNDNFTYAFDVYGYNEALEC
                     VPECAYEKGVSCFGFKNNVPVLDSIELLRSFCARIKDKCYIIEAKQISTGNDSEPLVK
                     NVQIVEEWQTTEKERAQLAHNILCAMFSNVKKLSEDDCTDYILYSFTDWKTNKKSYVF
                     NGFEYYN"
     gene            complement(4390..4590)
                     /locus_tag="ACZPDN_08350"
     CDS             complement(4390..4590)
                     /locus_tag="ACZPDN_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298675.1"
                     /translation="MDFVNGSKALVVLGRTKRFCCNAFEVYDFLIENGIVWELAFEAR
                     VWVQTADIGETYNENGFDIYIE"
     gene            complement(4604..4765)
                     /locus_tag="ACZPDN_08355"
     CDS             complement(4604..4765)
                     /locus_tag="ACZPDN_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298676.1"
                     /translation="MEISRIKKILDAHSTPYYIKGGRIFADCVFAFHEKFEDVTGWSK
                     SQLYAWLGY"
     gene            complement(4853..5026)
                     /locus_tag="ACZPDN_08360"
     CDS             complement(4853..5026)
                     /locus_tag="ACZPDN_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298677.1"
                     /translation="MNRLEEAKKAFLEVRSILTEKHEDFALAKAYKKPWKWYKEHTTQ
                     EAIKILRAEAKAN"
     gene            complement(5109..5549)
                     /locus_tag="ACZPDN_08365"
     CDS             complement(5109..5549)
                     /locus_tag="ACZPDN_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298678.1"
                     /translation="MKTIKLQGIHTPQKAIPAAELKPGMVTVWNFGYTSTVKSVEPTK
                     SGKSVKCVIISDESGNEHTRTMRADRLVGVKEEEPKNPIDKALASRQKTYSGIYSDVG
                     TALDTFSTSELAEYYIQRFGGSALRYFLEQGIISAEISKEKEAI"
     gene            complement(5636..5890)
                     /locus_tag="ACZPDN_08370"
     CDS             complement(5636..5890)
                     /locus_tag="ACZPDN_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298679.1"
                     /translation="MIIGLLENGNKCVYDLPTEIKTAAELKALIYGYNNGRMAESQRE
                     ELYNQPKLLGLNGPMWNGWGTLASTGETVAIIRYEKPVKY"
     gene            complement(5887..6054)
                     /locus_tag="ACZPDN_08375"
     CDS             complement(5887..6054)
                     /locus_tag="ACZPDN_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298680.1"
                     /translation="MDKQYRLVTESGKILLGGETYSRRGAESWFDGFNGIYEDDETGA
                     EERIYIEEVTE"
BASE COUNT         2046 a         1169 c         1221 g         1993 t
ORIGIN      
        1 aataaatgga ggtaaacaag atgtttaatt caaacggagt tagtctcgca gatattgccg
       61 cagtaacagg caataatcgt aataacgatg gtatgtgggg caatggtgca tggtggattg
      121 taattctctt aatctttggc tggggaaata acggctgggg cggtttcggt ggaaatggca
      181 acggcgcagg ctacactgat tcagctatac agaggggttt tgacaatcag gcagttatca
      241 gcaagttaga tggcatttct aacggacttt gtgatggttt ctacgctatg aacaacagta
      301 tgctcactgg ctttaatggc attaacacaa atattatgca gacaggctat ggcatacagc
      361 aggctatcaa tgctgataca gtcgctaata tgcagaatac aaatgcatta caggcgcagc
      421 tcgctaactg ctgctgtgaa actcgtgaag ctatccaagg tgtaaactac aacatggcaa
      481 ctaacacttg tgctttacag aacacaatga acaataatac aagagatatt attgacagcc
      541 aacaggcagg aacaagagct attcttgatt acttatgtac aaaggaaaat gcagatttga
      601 gagataaggt acagaaactt gagctttctg cttcacagga tagacagaat gcacttctga
      661 ctactgcaat gacagcacag acacagcaga ttgtcaactc tgtaaaccct acagctattc
      721 cagcttatgt tgtgcctaat cctaacgctt atgcttatgg atacggttgc aataccggct
      781 gtaattgcta aaactgaata attgagtatc ttaattgagt ttaactcgat tatgtctgct
      841 aagcagtatt acttataatc aaagggcaga ctataatgtt tgcccttatt tttatgaaag
      901 agaggtaaag ataatggaaa taacaggaat tgcattacaa acagttgccg ccggagaaga
      961 tgttgcattt acagaaacac cagtatgcgg aactaaatgt atagtccaca gacaaggaag
     1021 cggaattatc aagttaagag gtattacaaa tcagtgcaag gctagatttt tagtatctta
     1081 tagcggaaac attcagatac ctacaggcgg tacagttgga gctatatcac ttgccattgc
     1141 agtagatgga gagcctttgc agtcaacacg aatggttgta acgccagccg cagttgaaaa
     1201 tttatttaac gtatcagcac aagcatatgt tgatgtacct tgtggctgct gcagtacagt
     1261 agcggtgcag aatacatcta cacaggctgt tgaggtgcag aacagcaact taatcgcagt
     1321 aagggaggct tgacgttatg catattgaaa gaatacacaa aatggttgag tgccttaccg
     1381 aaaagacact atctgaactt gataagggca ttgaaaatgt aaatgttgag gaaatgtcag
     1441 aagctgtgga tatgattaag gatttatgtg aagctgaata tcgtgcagtt atcgttaagt
     1501 ctatgaaaaa ggctgatgaa gaggaagaag agtacgacaa agagctccta agaagtctta
     1561 agacagaata tggcgaagaa aatggcagaa gattttacga ccactaccgc tatgctaatg
     1621 gcagattcgc cccaaaaggt aaaggaacat accgcagagg atatgaagaa ccgccttata
     1681 tgcacatgta cccagaagca gagcatatga gggatatgga tagagattat ggcaagatgt
     1741 actatacaga gccaatgaac gaaagcggct atgacagagc aaagagaaat tatacagaaa
     1801 ctaaggaaat gcacagtggc actacgccag aggataaaga acaaaggatg aaagcgttag
     1861 ataaatacat caaaactgtc actaacgaaa taattaaaat agttagtgat aacgcaacaa
     1921 ccgaagaaaa gagtttaatt aagaataata tgaataatct tatgtcaaaa ttctaagatt
     1981 aagggctatg agtagcaata ttcatagcct atttcattca gaaaggagca tacagatgat
     2041 ttttaatatt aatggcacaa tgtggcgagt acaatatgaa aattcaaatt caagcgaatt
     2101 aaagcggtca gacaatgttt ctgtgctagg tgtaaccgat agaaatacac atacaattta
     2161 tctatcaaat gccttgcgtg gatttatgca acgcaaagtg ctgatacacg aagtatgtca
     2221 cgcaatctgt atgtcctatg atgtgtattt gcctattgaa caagaagaga tattgtgcga
     2281 ttttgtagca acttatggag atgaagtgtt tgacattgtt gatatggttt taggagcagc
     2341 taggagagtg ggatgatgag tatagatgag ttgttaaaga taattcaaaa gactaatccg
     2401 actatgacaa aggaaatatt aatatatgag cttagtcaat gccggtattc aagtaaagca
     2461 ttgatttata cagaaaaatg ttgtattgac agtaatgctt aaaaatgcta ttatttaata
     2521 gatgtaaaca attgataatt aatatatcat ttcaccttaa tagaaccata gtggaaagtt
     2581 gcattgatac atttttgtat aggtgcaact tattttattt tagaggtttt attatgagag
     2641 ttgtaagatt aaaaatgtat caagaaatgg ctagattcaa taatccatca gcgccaaaag
     2701 gtgcagattg ctaccctttg ccaccattta gcacagttaa tgggtttatt cattcaatgt
     2761 gtcaatggaa aaggtatcat aaattagatt attttgttac tggcaaagga atttataata
     2821 ctaaggtgca aaaagaatgg cacggtggct ataatttcaa caaaattagc gatgaaatgc
     2881 ttaagcgttg ggatgttata acagattatg cagacggaag ccataccggc tgggttagta
     2941 cagttaaata tcatctaatg ctagttgatt tatatacaac tatatacatc aaagctgatg
     3001 atagtgacat agatgatata taccatgctt tactaaatcc gccggtatat ccatcattgg
     3061 gcgaatatgg tgatttatgt aagattgaag cagtagatat tgtagaactt aaggagcttg
     3121 ataaatgtgt acctgctcca cttgctatgc aatcttatat tcctgttaat aaaggcaatt
     3181 ttgcaggaac tatatataga attaataaca aatacgaaat cattgagggt cttaggcgat
     3241 ttcagaaagt ttcttgttat ttagtggata aagggcaaga agttgtgagc aatctttttg
     3301 atgatgataa gccaattatt tttataaact aatttaaaac ccacggaata taggtaaaat
     3361 ttttctttac ccccgtgggt tgactttttg tattcacaat ttcattttta aaaaatctca
     3421 aaatttggtt tagattttgt ttaaatctta ctctaaaaat tgaaaaaatt ttctcataaa
     3481 atataatgtg aaatttttga aacccccggt catatgcaat tttgtattca aaaatccgtg
     3541 aaaaactttt cccaaaattt gacctcaatt ttgttcagat ttgcccttga aaattgatga
     3601 aaaactttag cagggtaaag caccatatat aaactttgct ggctgcggtt cgtgtttatt
     3661 ttggctttgt ggctttgtga tttgccctgt gcggcggttt tattgtaccg atgtagattt
     3721 ataagcttac aaagtaaaac agccttaaaa cgctttcaat tataatattc gaagccgtta
     3781 aaaacatagc ttttcttgtt tgtcttccag tctgtgaagc tgtacaatat ataatccgtg
     3841 caatcatctt ccgacagctt tttaacattg ctaaacatgg cgcataatat attgtgtgca
     3901 agctgtgcgc gttctttctc cgttgtttgc cattcttcca caatctgcac atttttaaca
     3961 agcggctcac tgtcgttgcc tgtgcttatc tgcttagctt cgataatata acatttatct
     4021 tttattctag cacaaaaaga gcgtaaaagc tcaatagaat caagaaccgg tacattgttt
     4081 ttaaaaccaa agcaagaaac acctttttca tatgcgcatt ctggcacaca ttccaacgct
     4141 tcgttatatc cgtatacatc aaatgcatat gtaaagttgt cgttctggtc gtttgtcatt
     4201 tttagaaaat tgatagattt ttcatttttg gggatttccc cgaaccttaa aaaatgcata
     4261 aaatgccgcc tttcaaatat tataatatta taccatatta ttattaatat gggaatgctt
     4321 gcggctggaa tcgaaccaac cagaacccac agcaagccaa aaaggacgca gattgtacgc
     4381 ccttttgaat tattctatgt aaatatcaaa accgttttca ttgtatgttt cgccaatatc
     4441 cgccgtttga acccaaaccc tcgcttcaaa tgctaattcc cacacaatac cattttcaat
     4501 taaaaaatcg taaacctcaa aagcgttaca acaaaatctt tttgtgcgcc ccaatacaac
     4561 aagcgcctta gatccgttta caaagtccat aatatacccc ctgttaatat cctagccatg
     4621 cgtaaagctg cgacttgctc caacctgtta catcttcgaa tttttcgtga aacgcaaaca
     4681 cgcaatctgc gaaaatgcgc ccacctttaa tgtaatatgg cgtgctgtga gcgtctaaaa
     4741 tcttcttaat tctactaatt tccatataat ttaaaacctc catattctta atattatccc
     4801 ttataggaaa aaccgccgcc ggcatcggtc cggctggcat tctctgcggc ggttagtttg
     4861 cttttgcttc tgctcttaaa atcttaatag cttcttgcgt ggtgtgttcc ttgtaccact
     4921 tccaaggctt tttatatgct tttgcaagcg caaagtcttc atgtttttct gtcaaaatgc
     4981 ttctaacttc taaaaatgcc ttttttgctt cttctaatct gttcataatg tttttacctt
     5041 tcttttattt attcccttac gggtaaagca agccggggaa tcgaaccccg ggaaagccga
     5101 ccttgctatt atatcgcttc tttttctttg ctaatttctg cggaaattat tccttgctcg
     5161 aggaaataac gcaatgcgct gccaccaaaa cgttgtatat aatactctgc aagctccgaa
     5221 gtactaaaag tgtctaatgc tgtgccgaca tcgctatata ttccgctata tgttttttgt
     5281 ctgcttgcaa gtgccttatc aattggattt tttggctctt cctctttaac tcctacaagc
     5341 ctatcagctc gcattgttct agtgtgttcg tttccgcttt cgtcggaaat aataacgcat
     5401 ttaacgcttt ttccgctctt ggtaggctct acgcttttaa ctgtggaagt gtaaccaaaa
     5461 ttccaaactg taaccattcc cggctttaat tctgccgccg gaattgcttt ttgcggtgtg
     5521 tgtattcctt gtagtttaat tgttttcata atattaacca tcctttcatt atgcgccctg
     5581 tctcatcagt gcaggtgggg cagttcctgc agaccgccaa aagtggcggt ttcgactagt
     5641 atttgacagg cttttcataa cggataattg cgaccgtctc gcccgtgctt gcaagagttc
     5701 cccagccgtt ccacatagga ccatttaagc ctaataactt aggctggtta taaagttctt
     5761 ctctctggct ttctgccatt cttccgttat tatagccata tataagagcc ttaagctctg
     5821 ctgctgtctt aatttccgtc ggcaaatcgt aaacgcattt attaccattt tctaataacc
     5881 ctattatcat tctgttacct cctcaatgta tattctttct tctgctcctg tttcgtcatc
     5941 ctcatagatt ccattgaaac catcaaacca gctttcagct cctcggcggc tgtatgtctc
     6001 accgcctaat aaaattttac cgctttctgt tacaagtctg tattgtttat ccatgtttgt
     6061 gtcctcactt tctttaattg catgattata ataacatatt gcttttataa ttgcaatagt
     6121 taattgcaat aaaaacaaaa taaattattg caatttaaaa ttgatatatt agtagagaca
     6181 attagtactt gacatatagc aattatttat tgtattttat aattgcaata ttaaattaca
     6241 atataaaaga ggtgcttata tatgattaaa acagataaag aaattgcaga gaaggcaaga
     6301 gagagagcaa gaaagcagaa cgaagccgca aaaaacaact gggactgtat tacttgcaga
     6361 cttccaaaag gcacaaaaga acgcatacag gcgcaagggc taacaattaa cggatttgtc
     6421 aatcagtta
//
LOCUS       DBMDWL010000120         6401 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4689_length_6401_cov_2.742381, whole genome shotgun sequence.
ACCESSION   DBMDWL010000120 DBMDWL010000000
VERSION     DBMDWL010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4689_length_6401_cov_2.742381"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="ACZPDN_08380"
     CDS             complement(<1..1135)
                     /locus_tag="ACZPDN_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013348.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR1298681.1"
                     /translation="MKISVEQLKQLNKEEYILIDIRDAEEAFKNPIENSIVLSGESVL
                     SKEFDSTKKIIVVCAQGYVSDIVAQRLQEKYEAYSVDGGYVSIVMDKMNTNVSDDFCA
                     QVERSIIKTYRRKIWSKFTKAIRDYELVKEGDCIAVCISGGKDSMLMAKCFQELHKHS
                     PVPFDVKYIVMDPGYSKENRDVIERNAQRLNIPIEIFESDIFDNVFNIEKNPCYICAR
                     MRRGHLYNYAKNLGCNKIALGHHYDDVIETILMSMLYGGQIQTMMPKLHSNNFKGMEV
                     IRPLYLIHEDDIKAWAKHNNLYFIQCACKFTQACANETCLTQSKSKRLEMKNLIKELK
                     KVNPFVEQNIFKSVENVSLKTVIAYKDDHARHHFLDTYDEEGNE"
     gene            1210..2535
                     /locus_tag="ACZPDN_08385"
     CDS             1210..2535
                     /locus_tag="ACZPDN_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298682.1"
                     /translation="MTQDMTQGKIMPMLVHFTIPLVLGNLFQLTYNVVDSIIVGHFVG
                     KEALAAVGICNPITTLFILFLNGLCMGASILIGMYYGAKEEEKLARQMSTTMLAGLVF
                     SLVLTLVCIFFSPQILKFMQVDLSILNVTTTYLQIIFAGLVFTFLYNFFASTLRALGD
                     SKSPLYFLIISSILNIFGDLFFVVVLHAGSKGCAISTVISEALCCLFCILYIQKKVPL
                     LQLGKKWLVFDISLLKRTIAYGWASAMQQATVQLGKLGIQMIVNTMGVSVVAAFTAVN
                     RIDDFAYTPEQNIAHSMTALMAQNKGAGNNNRMQEGFRCGMVLEFIYGIFIFLVCFIF
                     AKPLMCLFVQDEEVIMHGVTYLHLISIMYILPAFTNGIQGFFRGIGDLKITLISSLVN
                     MTVRVIVAAPLVFSLHLGIEALPYSYLAGWIGMLVAELPLLIRTYKAYK"
     gene            2811..3344
                     /locus_tag="ACZPDN_08390"
     CDS             2811..3344
                     /locus_tag="ACZPDN_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR1298683.1"
                     /translation="MILICVYFFSQGYFTNPQLLQATLSKAGILAPLLFILLQIIQVI
                     IPIIPGGASSALGITAFGAVNGFIYNYIGICIGSICIFLLVRKYGQRIILKLCKKKDY
                     DKYMKYTYDQNKFDTFFMLAIFLPCAPDDFLCMLAGLTHMSLKKFVWIILLGKPLSLI
                     AYSYGLTQLFQIIERWL"
     gene            3413..4114
                     /locus_tag="ACZPDN_08395"
     CDS             3413..4114
                     /locus_tag="ACZPDN_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1298684.1"
                     /translation="MEHTILVVEDEQGIREAIGIYMKNQGYNVILATNGKEGLEKIET
                     NDIHLAIVDIMMPIMDGISMVMEVRKHHDFPILFLSAKSEDIDKITGLNIGADDYITK
                     PFQSMELVARVRSHLRRYEQILNLKNETKNEDEYMIGDLVLNNTTKRVTLNSRDIKLT
                     PKEFDILKLLISHPGQVFSSQQIYELVWKEEAINTETIMVHIRKLREKIEANPKKPIY
                     IKVVWGQGYKMENIQ"
     gene            4111..6150
                     /locus_tag="ACZPDN_08400"
     CDS             4111..6150
                     /locus_tag="ACZPDN_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR1298685.1"
                     /translation="MKKKNIFLTILCALCIISSCIVTSFYPNVSTATPTKLPETLEQN
                     LTAFILAKKLEQDPKYEYITFEKDTPEDIQKDIKKGLNSSLTSAKNIFENDPNFFYTC
                     DVNNKITSKQFNVNVKKKDTKYYDTLDSNTLNVTDNIVNLINYNSEKYYEYCDGTYYC
                     DGEPFPGYTLHMPSDVVLTFYIPAVLNYDNTSLIDLLDLDADQYTYFFVTSFLICSAI
                     IALYVFLNKYAYEKEAYIFRHVNNWLFEPAFFLFLVVDVLLASGTCILTTYSIEGTFL
                     HILNRYHIELSQPIVYGANILAWSITLFFIGLSVYWLKCQFTTSIKDWFFHRTLIGNF
                     ILYFSNKVEQIISTDLSDKILKKYIIFSTCLILILAFISLLNIPFFSFFVVVTALIGI
                     SVVGYKKIKNVQSQYQDILHMTEDLSSGNFENIKPANSGLFQSLNNNIYQIKDGFKQA
                     VKEQVQLQNLKTELISNVSHDLKTPLTGIKNYVELLNDPSISEEDKKSYLERLNQYTD
                     RLSILITDLFEVSKANSGNIDLEYSDINIIEFMEQVCAENEELLQAKNLQLVTNLPEK
                     EIHVCLDGNKTYRIFENLFTNISKYALENTRVFLDVKDIGNSVVITMKNTSKAPLDFD
                     SDITERFVRGDKSRHESGSGLGLAIVKSFTEVQNGTFMIETDGDLFKSILSFHKS"
BASE COUNT         2113 a         1053 c         1017 g         2218 t
ORIGIN      
        1 attcatttcc ctcttcatca tacgtatcta aaaaatgatg tcttgcgtga tcatccttat
       61 aggcaatcac ggttttaaga cttacatttt ctacactctt aaaaatattc tgttcgacaa
      121 acggattgac tttcttcaat tccttgatta agtttttcat ttctaaacgc tttgacttag
      181 attgtgttaa acatgtttca ttagcacaag cctgagtgaa tttgcatgca cattgaatga
      241 aatacagatt gttatgttta gcccaagcct taatatcatc ttcatgaatc aagtataaag
      301 gtcgaatgac ttccataccc ttaaagttat ttgaatgtaa ctttggcatc atcgtttgaa
      361 tttgtccccc ataaagcata gacattaaaa tggtttcaat cacatcatca taatggtgtc
      421 ccaacgctat tttattacac cctaaattct tagcatagtt atataaatga cctcttcgca
      481 ttcttgcaca tatataacat ggattctttt caatattaaa aacattgtca aaaatatcac
      541 tctcaaagat ttcaattgga atatttagcc tttgggcatt cctttcaatc acatcacgat
      601 tttcttttga ataacctgga tccatcacaa tgtatttcac atcaaaagga accggtgaat
      661 gtttatgcag ttcttgaaaa cactttgcca tcaacatcga atcttttcca cccgaaatac
      721 ataccgcaat acaatctcct tctttgacaa gctcataatc acggatggct ttggtaaatt
      781 ttgaccaaat ctttcttcga tacgttttaa taatacttct ttcgacttga gcgcaaaaat
      841 catccgacac atttgtgttc atcttatcca taacgattga aacatatcca ccatctacac
      901 tatacgcttc atatttttcc tgcaatcttt gtgccactat atcactgaca tagccttgtg
      961 cgcaaacgac aataatcttc ttagttgaat caaattcttt ggataaaaca ctttctccac
     1021 ttaatacaat actgttctcg attggatttt taaaggcttc ttcggcatca cgtatatcaa
     1081 ttaagatata ttcttcttta tttaattgtt ttaattgttc tactgaaatt ttcatgagac
     1141 tattctaaca taatattgaa tttttggatg aaaaagcaca aaatatgaac aaaaaaagga
     1201 aatttgatta tgacacaaga tatgacacaa ggcaaaatca tgcctatgtt agttcacttt
     1261 accattccct tagtattggg aaatctattc caattaacat ataatgtcgt tgacagtatt
     1321 attgtaggcc actttgtggg gaaagaagct ttagctgctg tcggaatttg taatccgatt
     1381 acgacattat tcatcctatt tctaaatgga ctttgcatgg gtgcaagcat attgattggc
     1441 atgtattatg gggctaaaga agaagaaaaa cttgctcgtc aaatgagcac gactatgctt
     1501 gcagggcttg tattttcact tgtattaaca cttgtctgca tattcttttc accacaaatt
     1561 cttaaattta tgcaggtaga tctatccatt ttaaatgtga ctactaccta tcttcaaatt
     1621 atctttgccg gtttagtctt taccttttta tacaactttt ttgcgagtac actacgcgct
     1681 ttaggcgaca gtaaatcccc cctatatttc ttaattatca gttccattct aaatatcttt
     1741 ggagacttat tctttgtggt tgttttacat gcaggaagta aaggatgtgc catttctact
     1801 gtgattagtg aagctttatg ttgcctattc tgcattctat atattcaaaa gaaagtgcca
     1861 cttctacaac tgggtaaaaa atggctggta tttgatattt cattattaaa aagaaccatt
     1921 gcgtatggct gggccagcgc aatgcagcag gcaactgtac aacttggaaa gcttggtatt
     1981 caaatgattg tcaatacgat gggcgtatcg gttgtagctg cctttacggc cgtcaatcga
     2041 attgatgact ttgcgtatac ccctgaacaa aacattgctc actctatgac cgctttaatg
     2101 gcacaaaaca agggtgccgg aaataacaat cgtatgcaag agggatttcg ttgcggaatg
     2161 gtcttagaat tcatctacgg tatatttatc tttctagtat gttttatctt cgcaaaacca
     2221 ttaatgtgtc tatttgtaca agatgaagaa gttattatgc atggagtgac ttatctacat
     2281 ttaatttcca tcatgtatat tcttcctgcc ttcaccaatg gaatccaagg tttctttaga
     2341 gggattggag atttaaagat tacactcatt tcaagtctag tgaatatgac agttcgagtt
     2401 attgtggctg ctccacttgt atttagctta catctaggca tcgaagcttt accatactca
     2461 tatctagctg gatggattgg tatgttggtg gctgaacttc cattgcttat tcgtacatac
     2521 aaagcttata aataagactt gtgtataatt gacacaagtt ttttgttttc cttttatata
     2581 aagcatttat tacaatttac actatttttc caacccatat ttttgtattt catccacttt
     2641 tccaaccttt tttatgccat ttttaacaac ttttccaact ttttgattca attatttaga
     2701 aaactttaag atttatcacc atgattaata tttattcgtt atattaatca tggtgataat
     2761 ttatgacaaa taaaaacata caaaacataa cacattttgg atttatcctt atgattctta
     2821 tttgtgttta tttcttttca caaggatact tcacaaatcc acagcttctg caggctacac
     2881 tgtcgaaagc cggaatttta gcacccctac tttttattct tctacaaatc attcaagtga
     2941 ttattcctat tattcccggt ggtgcatcaa gtgccttagg tattacagct tttggagcag
     3001 taaatggatt tatatataac tatataggta tttgtatcgg atccatatgc atctttttac
     3061 ttgttcgtaa atatggtcaa agaatcattc taaaattatg taaaaagaaa gattacgaca
     3121 aatatatgaa gtatacatat gatcaaaata aatttgatac cttctttatg ttagctattt
     3181 tcttaccttg tgcacccgat gatttcttgt gtatgcttgc aggactgaca catatgtcgc
     3241 tcaaaaaatt tgtctggatc attcttttgg gtaaaccctt atctttgatt gcctattctt
     3301 atggtcttac gcagttgttt caaattatcg aaaggtggtt ataattatgt tatacattag
     3361 aatattatgt gcatgcattg gattttattt acattattaa aaggagaact ttatggaaca
     3421 tacaatatta gtggtagaag acgaacaagg gattcgtgaa gccatcggaa tttatatgaa
     3481 gaatcaagga tacaatgtga ttcttgccac aaatggaaaa gaaggcttgg aaaagattga
     3541 aacaaacgat attcatttag ccattgtaga tattatgatg cctattatgg atggcatttc
     3601 tatggtcatg gaagtaagga aacatcatga ctttccgatt ctatttttaa gcgcaaaaag
     3661 tgaagatatc gataaaatca caggtttaaa tattggtgcc gatgactata tcaccaagcc
     3721 atttcaaagt atggagctag tcgcaagagt acgttcgcat ttaagaagat acgaacaaat
     3781 cttaaatcta aaaaatgaga caaagaacga agatgagtat atgattggtg atcttgtgtt
     3841 aaataatacg acaaaaagag tgacgttaaa ttcaagggat attaaattaa cgccaaaaga
     3901 gtttgatatt ttaaaactat tgatttcgca ccctggacaa gtcttttcta gccaacaaat
     3961 ctatgaattg gtatggaaag aagaagctat taacacagaa acgattatgg ttcatattcg
     4021 taaattacgt gaaaagattg aggccaaccc taaaaagcca atctacatca aggttgtatg
     4081 ggggcagggc tataaaatgg agaatataca atgaaaaaga agaatatttt tttaacaata
     4141 ctatgtgctt tatgcatcat tagttcttgt atagtgacaa gtttttatcc aaatgtttct
     4201 acagctactc caacaaagct tcctgaaact ttagaacaaa atcttacagc ttttattcta
     4261 gctaaaaaat tggaacaaga tccaaagtat gaatatatta cttttgagaa agatacaccg
     4321 gaagatattc aaaaggatat aaaaaaaggg ttaaactcgt ctttaactag cgcaaaaaat
     4381 atttttgaga atgatccaaa cttcttttat acgtgtgatg tgaacaataa aatcacttca
     4441 aaacaattta atgtgaatgt aaagaaaaaa gatacaaaat attatgacac tttggattcg
     4501 aatactttaa atgtaacgga taatattgtg aatcttatta actataacag tgaaaagtat
     4561 tatgaatact gtgatggcac atattattgt gatggagagc catttccagg ctatacactt
     4621 catatgccaa gtgatgtcgt attaacattc tatattccag ctgttttaaa ttatgataat
     4681 acaagtctaa ttgacctctt agatttagat gccgatcaat atacctattt ctttgtgact
     4741 tccttcttga tttgtagtgc cattatcgct ctatatgtct tccttaataa atatgcgtat
     4801 gaaaaagaag cctacatctt tagacatgtg aataactggt tatttgaacc tgcattcttt
     4861 ttatttttag ttgtcgatgt cctactcgct agtggtacat gcatattaac tacctattcg
     4921 attgaaggta cattcttaca cattctaaat cgttatcata ttgaattgag tcagcctatc
     4981 gtatatggag ccaacattct tgcgtggtcc atcactctat tctttatcgg tttatccgta
     5041 tattggttaa aatgtcaatt tacaacatcg attaaagatt ggttctttca tagaacttta
     5101 attggtaact tcattcttta tttctcaaat aaagtggaac aaatcatttc aaccgattta
     5161 tcggataaaa tccttaaaaa atatattatt ttctcaacat gtctaatttt gattcttgcg
     5221 tttatatcat tgcttaacat tccattcttc tctttctttg ttgtcgttac agcattaatt
     5281 ggcattagtg ttgtgggcta taagaagatc aaaaatgtgc aaagtcaata ccaagacata
     5341 ttacatatga cagaagatct atcgagtggt aattttgaga atatcaagcc tgcaaattct
     5401 ggtttattcc aatctttaaa taataatatc tatcaaatta aggatggttt taaacaagct
     5461 gtaaaagaac aagtacaatt acaaaatcta aagacagaat tgatttcaaa tgtatcacat
     5521 gatttaaaaa caccattaac aggtattaaa aattatgtag agttattaaa cgatcctagt
     5581 attagtgagg aagataaaaa gagttattta gaacgattga atcaatatac agatcgttta
     5641 agcattctaa tcacagattt gtttgaagta tccaaagcca atagtggtaa tattgattta
     5701 gaatattctg atattaacat cattgaattt atggagcagg tatgtgctga aaatgaagag
     5761 cttctacaag ctaaaaatct tcagcttgtg acaaatcttc cagaaaaaga aattcatgta
     5821 tgtttggatg gaaataagac atatcgtatc tttgaaaact tatttacgaa tatttctaag
     5881 tatgcacttg aaaacacacg tgtattcttg gatgtgaaag atattggtaa ttcagttgtg
     5941 atcacaatga aaaacacttc aaaagcaccg cttgattttg atagtgatat caccgaacgt
     6001 tttgtgcgtg gagataaatc aaggcatgaa tctggaagtg gtttaggact agccatcgta
     6061 aagagtttta ccgaagtaca gaatggtaca tttatgattg aaacagatgg ggatctcttc
     6121 aaatccattc taagttttca taaatcctga aatatgtaga attctacaga aaaaatgatt
     6181 gtagataaaa taaagtggtc aaaaatccta aagtaaagga tacaggccac tttttatctg
     6241 tgtatcatta atttatagtc agaaaaaata tagaattcta caaaaaaatg attagaaatg
     6301 ataaaatcag aattattttg gcacacttac cagtaaatcg atattacctt ggatggagta
     6361 tttttactgg taagtgtgcc aaaataattc tgattttatc a
//
LOCUS       DBMDWL010000121         6354 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4749_length_6354_cov_2.131297, whole genome shotgun sequence.
ACCESSION   DBMDWL010000121 DBMDWL010000000
VERSION     DBMDWL010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4749_length_6354_cov_2.131297"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            53..346
                     /locus_tag="ACZPDN_08405"
     CDS             53..346
                     /locus_tag="ACZPDN_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR1298686.1"
                     /translation="MSTIQVRVDDDLKSKADALFKELGTDTTSAIRMFLTQAVAYDGI
                     PFEIKKFNKTKEMKIMTEDEFLDRLAYSRAQSKEGKVIDADIAIDSIRNKYGL"
     gene            336..650
                     /locus_tag="ACZPDN_08410"
     CDS             336..650
                     /locus_tag="ACZPDN_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR1298687.1"
                     /translation="MDYKVIFTENAMKDMDSIVEYLLFHLRNKQAANHFIDSVKNGKF
                     VLSNSAESFQLCLNPRLRSKGYRRLNLDKTKYFLLYRIENSIVFIDGIYHQFQDYENK
                     IK"
     gene            complement(888..2624)
                     /gene="aspS"
                     /locus_tag="ACZPDN_08415"
     CDS             complement(888..2624)
                     /gene="aspS"
                     /locus_tag="ACZPDN_08415"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1298688.1"
                     /translation="MYRTHNNGELRLQDVNSEVTLCGWVSKRRNFGALVFIDLRDRYG
                     ITQLVFNEDIAAQISDVRNEYVLQVKGTVVERKDKNPKLETGEIEVVVSEVKIVNTAI
                     TTPMIIADETDALEDTRLKYRYLDLRRPVLQKNLILRNRITLLVRNYLAKYGFTEVET
                     PILCRSTPEGARDYLVPSRISKGQFYALPQSPQLYKQLLMVGGMDRYFQIARCFRDED
                     LRADRQPEFSQIDIEMSFVDEEDIWSMTEGLMKEIFKDIKGIELPEFKRIPYDTCMEK
                     YGSDKPDLRFDMPLYNVSEVFANTEFKVFENCLNEGGIIQAMNVKNGADKFSRKQLDK
                     LQDYVKVYGAKALANLKLTSEGFAGSVTKVLSDAEKEALRTMLNIEENDIVFFVADKK
                     KVAQTSLGALRVKLGHDLDLINKDAYEFLWVTDFPMFEYDENENRYVAAHHPFTSPNL
                     EDVDKLMSDPAHCYSRAYDLVLNGYELLSGSIRIHDQKLQEKVFEAIGMTLEEAHEKF
                     SWFMDAFQYGTPPHGGVGIGLERLTMILAGTDNIRDVVAFPKTASASDLMAQAPSPVD
                     PAQLKELGIETK"
     gene            complement(2627..3937)
                     /gene="hisS"
                     /locus_tag="ACZPDN_08420"
     CDS             complement(2627..3937)
                     /gene="hisS"
                     /locus_tag="ACZPDN_08420"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR1298689.1"
                     /translation="MAYQIPRGCQDVFGSDSYKWQALEQTLRSFCYMYNYDEIRTPIF
                     EHTEVFKRENDSSDMVNKEMYTFKLENSKTSLTLRPEGTAGVVRSFVENKMYANPDLP
                     AKFYYMGPMSRHERPQKGRMRIFNQFGVEALGVKSPYVDVETIVLAFSILKSLGLKDI
                     KVCLNTLGDDESRANYRSALKEYFAPYVDELCGDCKRRYEQNPLRILDCKVDHDHECM
                     KNAPKMSDYLNEASQEYFNTVCELLDKLEIPYEVDDKLVRGLDYYTHTVFEIVSMNSE
                     MGAQSTVLAGGRYDGLISYFGGPEGMSGIGWALGMERLLIALEAEGIELETKPALDAY
                     VMCLDPEARTYAFEVLTSLRAYGYRCDMDMMERSFKAQFKAVDRSKAKLAILIGKSEL
                     EEKKVTVKNIAHKEQESIEVEKLIPYVDSLLEEESHHHHEHEGE"
     misc_binding    complement(3976..4165)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     assembly_gap    4168..4177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4178..4856)
                     /locus_tag="ACZPDN_08425"
     CDS             complement(<4178..4856)
                     /locus_tag="ACZPDN_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1298690.1"
                     /translation="MGRHFEVRAAAMQKTANAKAKIYSRYSKEILVAAKNGDPNPDMN
                     QTLKKAIDRAKANNVPADVIKRAIEKAKSSADESYSSARYEGFGQGAGSTIIIDCLTD
                     NANRTIANLRACFNKSHAKLGVGGSVSFGYEHLGVIVIQYDNEEAMMDALLDAEIELN
                     DIEIEEGYMTITVDPTQLDDAKVVVEKLIPDVKFEVLEDKMIPNEEVELHGEDLTLFK
                     RLVTLLDE"
     gene            complement(4916..5902)
                     /locus_tag="ACZPDN_08430"
     CDS             complement(4916..5902)
                     /locus_tag="ACZPDN_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1298691.1"
                     /translation="MQIFFLRRRFVSFLEFDCSDRTFEEIQIFAGFQDSNLEMLNKCF
                     KSVFMIRDQKIKVEVNDTLDTHKVEEVIDACFSIIDTQHRLNSQDVNYICSLAFKNRL
                     KDFKAHQLQAVCKTKTGKMIYPKTIGQAILCDAMRHNEIVFATGVAGTGKTYLAVAYA
                     VNMLKSERVEKIILTRPAVEAGESLGFLPGDMKEKVDPYLRPLYDALNEMLGLETVEK
                     YVEKQVIEIAPLAFMRGRTLNDAVVILDEAQNATCAQMKMFLTRMGKNCKMVVTGDVT
                     QIDLIKKKDSGLMQACTILDHIDGISIIHLENSDVVRNPLVQKIIERYAKVE"
     gene            complement(5915..6094)
                     /gene="rpsU"
                     /locus_tag="ACZPDN_08435"
     CDS             complement(5915..6094)
                     /gene="rpsU"
                     /locus_tag="ACZPDN_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1298692.1"
                     /translation="MPKVVLKENEGLDDALRRFKRQVSRNGTLAEARKREFYVKPGVR
                     RKLKSEAARKARRGK"
BASE COUNT         1998 a         1264 c          907 g         2175 t
ORIGIN      
        1 atgtacatta gcaatgaatt tgcatataat ttaaatgtgg aggtgatgcc gtatgtctac
       61 tattcaagta agagtggatg atgatttgaa atctaaggct gatgcattat ttaaggaatt
      121 aggtacagat acaactagcg caattcgtat gtttttaact caagcagttg cttatgatgg
      181 aattcctttt gaaattaaaa aattcaataa aacaaaagaa atgaaaatta tgacagaaga
      241 tgaatttttg gatagacttg cctattctag agctcaatct aaagaaggaa aagtaataga
      301 tgcggatata gcaatagata gcattagaaa taaatatgga ttataaggta atatttactg
      361 aaaatgcaat gaaagatatg gattctattg tagaatacct tctttttcac ttgcgtaata
      421 aacaagcggc aaatcatttt atcgattctg ttaaaaatgg aaagttcgta ttaagtaatt
      481 ctgctgagtc atttcaactg tgtttgaatc cgagacttcg aagcaagggg tatcgaagat
      541 taaatttaga taaaactaaa tatttcttgt tgtatcgcat tgaaaattcg atagttttta
      601 tagatggtat ttatcatcaa ttccaagatt acgaaaataa aattaaataa ttcttctact
      661 agacgagtta cactcgtctt ttctagttat agcttctatt caacaattga acggtcattg
      721 attgcttatt taataaaaga aaagaatcgt tcttcgattt tttaaagcat gatacatgct
      781 aaaatgtacc aattcaagcg cagatggaaa aatgttacaa ttaataagcc tacatctaaa
      841 atcaaattcg ctctctttgt tgaaaaaaga caggaaaaaa tcctgtctta ctttgtttca
      901 atacctaatt cttttaattg tgctggatct actggacttg gagcctgtgc cattaaatcc
      961 gatgcacttg ctgttttagg gaatgccact acatcacgaa tgttgtctgt acccgctaaa
     1021 atcattgtta aacgttctag accgatacct acacctccat gaggtggagt tccatattgg
     1081 aatgcatcca taaaccagct gaatttttca tgtgcttctt ctaaagtcat accaatggct
     1141 tcaaagactt tttcttgaag tttttgatca tgaatacgaa tggatccaga taataattca
     1201 tatccgttta atactaagtc ataagctcta gaataacaat gtgctggatc cgacatcaat
     1261 ttatcgacat cttctaagtt aggagatgta aatggatggt gagccgctac ataacggttt
     1321 tcattttcat catattcaaa catagggaaa tcagtaaccc ataagaattc ataggcatct
     1381 ttgttaatca aatctaaatc atgacctaat ttcacacgca atgcacctaa agatgtttga
     1441 gccactttct ttttatctgc tacaaagaat acgatatcat tttcttcaat atttaacatt
     1501 gtgcgtaacg cttctttttc agcatctgac aatacttttg tgacactacc cgcaaaacct
     1561 tctgaagtta attttaagtt ggcaagtgct tttgcaccat atacctttac ataatcttgt
     1621 agtttatcca attgttttct tgagaactta tcggcaccat ttttaacatt catagcttga
     1681 atgatgccac cttcattcaa acaattttca aacactttaa attcagtatt tgcgaagacc
     1741 tcactcacat tgtataaagg catgtcaaag cgtaaatctg gtttatcgga accatatttt
     1801 tccatacaag tatcataagg gatacgctta aattctggta attcaatgcc tttgatatct
     1861 ttaaagattt ctttcattaa accttccgtc attgaccaaa tgtcttcttc atccacaaaa
     1921 ctcatttcaa tatcgatctg agagaattca ggttgacggt cggcacgtaa atcttcatct
     1981 ctaaaacaac gtgcaatctg gaaataacga tccattccac caaccatcaa taattgtttg
     2041 tatagctgag gtgattgtgg aagtgcatag aactggcctt tactaatacg actaggtact
     2101 aaatagtcac gtgccccttc tggtgtagaa cgacataaaa taggtgtttc cacttctgta
     2161 aaaccatatt tagctaagta gttacgaact aataaagtaa tacgatttct taaaattaaa
     2221 ttcttttgaa gaacaggtct tcttaaatcc aaataacgat attttaaacg cgtatcctct
     2281 aatgcatctg tttcatccgc aatgatcata ggtgtcgtga ttgctgtatt gacgattttt
     2341 acttcactaa ctacaacttc aatttcacct gtttcaagtt taggattttt gtcttttctt
     2401 tctacaacag tcccctttac ttgaagtaca tattcattac gtacatcact gatttgtgct
     2461 gcaatatctt cattgaatac aagttgagta ataccataac gatctcttaa atcaatgaaa
     2521 accaaagctc caaagtttct acgtttactt acccagccac ataaagtgac ttctgaattt
     2581 acatcttgaa gacgcaattc accattattg tgtgttctat acatttttat tctccttcat
     2641 gctcgtgatg atgatgactt tcttcttcta ataaagagtc aacataagga atcaatttct
     2701 cgacttcgat tgattcttgt tctttatgcg caatattttt tactgtgact ttcttttctt
     2761 ccaattccga tttaccaatt aaaatcgcaa gtttggcctt tgaacgatcc acagccttga
     2821 attgtgcctt aaaacttctt tccatcatat ccatatcaca acgatatcca taagctctaa
     2881 gcgaagttaa aacttcaaac gcatatgttc tagcttctgg atctagacac attacatagg
     2941 catctaatgc aggctttgtt tcaagttcaa taccttctgc ttctaaagca atcaataatc
     3001 gttccatacc taaggcccag ccaataccac tcatgccttc tggaccaccg aaatatgaaa
     3061 tcaatccatc ataacgtcca cctgctaaaa cagtcgattg tgcacccatt tctgaattca
     3121 tagatacaat ttcaaataca gtatgcgtat aataatccaa tccacgaact aatttatcat
     3181 caacttcata tggaatttcg agtttatcta ataattcaca tactgtgttg aaatattctt
     3241 ggcttgcctc atttaagtaa tccgacatct taggtgcatt tttcatacat tcatgatcgt
     3301 gatccacttt acaatccaaa atacgtaatg gattttgttc ataacgacgt ttacaatccc
     3361 cacacaattc atctacataa ggtgcaaaat attcttttaa tgcgctacga tagttagcac
     3421 gagactcatc atcacctaaa gtatttaagc atactttaat atcttttaat cccaatgatt
     3481 taagaattga gaatgcaagt acgattgttt cgacatcgac ataaggactt tttacaccca
     3541 atgcctctac accaaattga ttaaagatac gcattctacc tttttgagga cgttcatgtc
     3601 ttgacatagg tcccatataa tagaattttg cgggtagatc tggattggcg tacattttgt
     3661 tttctacaaa actacgaaca acaccagctg taccttctgg acgtaatgtt aatgatgttt
     3721 ttgagttttc aagtttaaaa gtatacatct ctttgtttac catatcactt gagtcatttt
     3781 ctcttttgaa gacttctgta tgttcaaaaa ttggtgtacg aatttcatca taattgtaca
     3841 tgtaacaaaa gcttcttaat gtttgttcaa gagcttgcca tttgtaagaa tcagatccaa
     3901 atacatcctg acaacctcta ggaatttgat atgccatgcg ttttcctcct ttaaataaac
     3961 aaaaaaacgc cctgatataa ggacgtttta aacgcggtac caccttattt tatacgtaaa
     4021 gtatacacct caagtcttaa cgcgaccaac ggttggtttt tgtccaactc ctccaaagtg
     4081 tcccaataag tattctacga aaattttcac caaccatttt ctctctacag tatatcctta
     4141 tcttcttctt tttcttagga ttaattannn nnnnnnnctt catctagtaa agttactaaa
     4201 cgtttgaata aagttaaatc ttcaccatga agttcaactt cttcatttgg aatcatctta
     4261 tcttctaaga cttcaaattt aacatctgga atcaattttt caacaacaac ttttgcgtca
     4321 tccaattgtg ttggatctac tgtaattgtc atatatcctt cttcgatttc aatgtcattt
     4381 aattcaattt cagcatctaa taaagcatcc atcatagctt cttcattatc atattgaata
     4441 acgatcacac ctaagtgttc atatccaaat gatacagatc cacctacacc taatttagca
     4501 tgtgatttat tgaaacaagc tcttaagttg gcaattgtac ggttcgcatt atctgttaaa
     4561 caatcgataa tgattgtaga tcctgcacct tggccaaaac cttcataacg agcacttgaa
     4621 taactttcat ctgcactact ttttgccttt tcgattgcac gtttgattac gtcagcaggc
     4681 acattgttgg ccttagcacg atcaatggct ttctttaaag tttggttcat atctggattt
     4741 gggtcaccgt tctttgcagc aactaaaatc tctttagaat atctagaata aatttttgct
     4801 ttcgcattgg cagttttctg cattgcggca gcacgtacct caaaatgtct tcccatagat
     4861 ttatcacctt tcaatatatt ttttacagtt tttaattata acagtcaaaa ccaaattatt
     4921 caaccttggc ataacgttca atgatttttt gaaccaaagg attacgaact acatcactat
     4981 tttctaagtg aatgatcgaa ataccatcta tatgatccaa gatcgtacaa gcttgcatca
     5041 agcccgagtc ttttttcttg atcaaatcga tctgtgtaac atcccctgtc acaaccatct
     5101 tacaattttt acccatacgc gttaaaaaca tcttcatttg tgcacaagtc gcattttgag
     5161 cttcatccaa aattacaacg gcatcattta atgtacgacc acgcataaaa gctaaaggcg
     5221 caatttcaat cacttgtttt tctacatatt tttcaaccgt ttctaaacca agcatttcat
     5281 ttagcgcatc atataaaggt cttaaataag gatcgacttt ttctttcatg tcacccggta
     5341 aaaaacctaa tgattcaccc gcttcaactg cagggcgtgt aagaataatt ttttcaacac
     5401 gttcactttt aagcatattt accgcatacg caaccgctaa ataggtttta cccgtacccg
     5461 caacaccagt cgcaaataca atttcattat ggcgcatcgc atcacataaa attgcttgtc
     5521 caattgtctt tggatatatc atttttcctg tttttgtctt acatacagct tgtagctgat
     5581 gcgctttaaa atctttcaaa cgattcttaa aagccaacga acaaatataa ttcacatctt
     5641 ggctatttaa acgatgttgc gtatcaatga ttgaaaaaca agcatcaatc acttcttcga
     5701 ccttatgtgt atctaaagta tcattcactt ctactttaat tttttggtca cgaatcataa
     5761 atacagattt aaagcactta ttcaacattt ctaaattgga atcttgaaaa cctgcaaaaa
     5821 tctgaatttc ttcaaatgtt cgatcggaac aatcaaattc taaaaaagac acaaatctcc
     5881 tcctcaagaa aaaaatctgc attaaaaatg cagattattt acctctacgt gctttacgtg
     5941 cagcttcaga ttttaacttt ctgcgtacac ctggttttac gtaaaattct cttttgcgcg
     6001 cctcagcaag ggtgccatta cgagaaacct gacgtttaaa tcttcttaaa gcgtcatcaa
     6061 gtccttcgtt ttctttaaga actacttttg gcatattcac aaacctccct tcccaagatt
     6121 agcaaaaata ttatatcaaa gatttcgtat aatgcaagtc tatttataaa attttaacct
     6181 aaatttgcct gaatacactc tttagccgct acattatcgc ctacccaagg agctcttccg
     6241 ttctcccgat aaacccgtct ttagaggttt gcaccatgaa tgtttagagg ctattctgag
     6301 aaaaagttga gattctccag tatttatcgt tgttttttgt gctttaaatt tatt
//
LOCUS       DBMDWL010000122         6172 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4956_length_6172_cov_2.383627, whole genome shotgun sequence.
ACCESSION   DBMDWL010000122 DBMDWL010000000
VERSION     DBMDWL010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4956_length_6172_cov_2.383627"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(165..749)
                     /locus_tag="ACZPDN_08440"
     CDS             complement(165..749)
                     /locus_tag="ACZPDN_08440"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1298693.1"
                     /translation="MTVLDTSIPYEKVLMVMARKTIRKEIELNPEFHYVEFDESLKEA
                     WCKLQTQVCLFENIDEARKKWDSMLTRDKDFFSKHFLFVENENNELVGSAGLWFGNDF
                     EESRLRVHYVAVSLSAQHKKIAQAMLTKLCMMYDMIPSKYPLYLATQSQSYGAIKLYS
                     RLGFTPYLGAYKGCTEQKSKNAWQNVTEILRCKA"
     gene            896..1570
                     /gene="deoC"
                     /locus_tag="ACZPDN_08445"
     CDS             896..1570
                     /gene="deoC"
                     /locus_tag="ACZPDN_08445"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR1298694.1"
                     /translation="MNKTQLANYFDHTFLKPYATEADLTKLCNEAKEIGAAMVAINTT
                     WTKFCKEQLKGTNVHVGAAISFPLGQAGLASKIAETKIAIEDGADEIDYVIDIGQAKM
                     HHWDYIKEEMESIVSICREHNVISKVIFENCYLEKDEIKQVALIAKEVKPDFIKTSTG
                     FGTGGATLEDVKLMVETVEGQVKVKAAGGIRDWKTCKAMIDAGAQRIGTSSSLKILEE
                     FEAEEK"
     gene            1572..2489
                     /locus_tag="ACZPDN_08450"
     CDS             1572..2489
                     /locus_tag="ACZPDN_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIR1298695.1"
                     /translation="MKPFLLEPIPDYTIWGTNHISKARGIDEDYGTWWEVSAHPYCSN
                     KIVGTDKTLMEMIQENPDEVLGKGYTLHETLRLSYLDTKDALSIQVHPEDDYALEHSN
                     DYGKYESWYILDAKPGATLVAGTTINDADEIKNALMNNDVEKYLNKVEVHKGDYIIIP
                     SGMLHALGKDILALEVGTNSNTTYRFYDYNRKGKDGKTRPLHVKESFDVTNFNLKPIF
                     VPQKQETHRIGNCPNFTVDEVYADSDITLTCEEHYMILSNIDSDCTIVWNSEEIVLPQ
                     YDSLFVPFASKKITIKQGAHLLVSNPRKD"
     gene            2493..3365
                     /locus_tag="ACZPDN_08455"
     CDS             2493..3365
                     /locus_tag="ACZPDN_08455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1298696.1"
                     /translation="MKTILVTGANGYIASLVRAYQKDNFNWICMTRKDADLTNPEDVK
                     RFVASQDFDICFHTAANATTAFCNEHPDLVHKINVESTQAIIDCCKEKGAKLIFCSTE
                     QAFNGKENHGPFKEDEPLSAVTVYGQNKIECEELIQKQLDDAIILRFTWMMGLSYPGI
                     KASPSIIKNVMNALINHTPTLFTVNEKRGMTYAKHLATQFDKITELEPGIYHVASKNT
                     MTTYESAKYVAKALGASDELIEEIILPNNERYQDRFRDYRLDSEKLESLGIHFATFEE
                     DVNEILMDFGWKKS"
     gene            3388..3804
                     /locus_tag="ACZPDN_08460"
     CDS             3388..3804
                     /locus_tag="ACZPDN_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HIR1298697.1"
                     /translation="MTILEENVKKYYQSGYNCSETLLHACNETYQLDISEEDMKMMAG
                     FGGGMFIGSTCGALVGSIAALSKIVCKTKAHEMLPELRPLIQKHTRNFKELLGNLDCA
                     HIKPVHHSTDPNIKCMNTCLLAAKALEKTMEEEHLL"
     gene            complement(3821..5047)
                     /gene="hflX"
                     /locus_tag="ACZPDN_08465"
     CDS             complement(3821..5047)
                     /gene="hflX"
                     /locus_tag="ACZPDN_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1298698.1"
                     /translation="MEKAILIGVQTYTSHSFEERMEEMKELALACDIEICMSVSQNLE
                     HPHKKYYMNPGKIEEIKRILEDQACSMVLVLEAISPSVQRNLSDFFDCEVMDKSALIL
                     EIFASRAKTKESMLQVECARLKYLLPRLQGSYKHMDRQRGGSKNKGTGEKKIELDARR
                     IESKIHFVEKELDKIHTQRSVQRNQRKKGMVKSVALTGYTNAGKSSIMNGFVNEEKSV
                     FEKDMLFATLTTSTRQIETENHNVFTLSDTVGFISDLPHSLVQAFHSTLEEAVVSDLI
                     LKVVDISDEHKDSKLQVCEETLKEIGCEKVPYLYVFNKCDKTNIEYPKRVENRIYICA
                     KEKSSIEFLKQEIEKELFHMETLHVVIPYEKVSVLDYIHTNGKVHKQEYKEDGIEVDF
                     SIQQDLIHRVDRLLRI"
     gene            5104..5979
                     /locus_tag="ACZPDN_08470"
     CDS             5104..5979
                     /locus_tag="ACZPDN_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HIR1298699.1"
                     /translation="MKIAYLMHYMKNNFEEITENLDILINQGDDVYLMVNDDDLRDDL
                     ILSYCDTPQLHVAHHQQTALPADMSLPRGFIVLMKDALEYEKEDGSFTYDRFIIMTDG
                     MIPTKSKAQLVEYLEAHEDEDIYYEVDNSKDNEEVKKRFENYAFFTNSYDFQKSKTIR
                     AMNKMTSSLVHSFKKREIEDTIYVSYPWFVLTHESAKALADNLAYCSNTFKMCLYPEE
                     MAIATMLHKFSNVDHHNENIWATGKDGEYKLQKPMENVELDAIKESTYFAALAHSEDN
                     LQVYEDYFDAYTCES"
     gene            5967..>6172
                     /locus_tag="ACZPDN_08475"
     CDS             5967..>6172
                     /locus_tag="ACZPDN_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024258.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1298700.1"
                     /translation="MRIIIVSDNHGNPDILKILYHIYSSLYKDNVKFIHLGDSQATNI
                     NDLNNFICVKGNNDTLDLPKFQRLT"
BASE COUNT         2170 a         1133 c          975 g         1894 t
ORIGIN      
        1 catatcctga ttttttcaat gaaaatgtaa ttccacgatt taaattttca tcatcttcaa
       61 ccacgagtat acctggcata gcatatgcac ctctctttat taactatata tatcagttgg
      121 gagatgaact tccatatttt atccctcaac ttccgattta tcaattaagc tttacaacgc
      181 aagatctctg tgacattttg ccacgcattc ttactctttt gttctgtaca acccttataa
      241 gcccctaaat agggtgtaaa gccaagtctt gaatataatt tgatagcacc ataactttgc
      301 gattgagttg ccaaatacaa tggatattta cttggaatca tgtcatacat catacaaagt
      361 tttgtaagca tggcctgcgc aatcttttta tgttgagccg ataaacttac cgctacataa
      421 tgaacacgaa gtctactttc ttcaaaatca tttccaaacc aaagtcctgc actaccaact
      481 aattcattat tttcattttc gacaaacaag aaatgtttag aaaagaaatc tttatctcta
      541 gtaagcatac tatcccattt ctttcttgcc tcatctatat tttcaaataa acaaacctgg
      601 gtttgtagct tgcaccaagc ttcttttaat gattcatcaa attcaacata atgaaattct
      661 ggatttaatt cgatctcttt acgaatggtc tttctagcca tgaccatcaa tactttttca
      721 taaggtatac ttgtatctaa tactgtcata aaactctcca tttcttttaa aattagtata
      781 tcataatctt cctacttcaa atgatataac agtttctttt taaatgttat aacatttgct
      841 tgacttctct ttttttacgt tctagaatga aaacgtaaaa gagaggtaaa caattatgaa
      901 caaaacacaa ctcgcaaatt attttgacca cacattttta aaaccttatg caactgaagc
      961 agatcttact aaattatgta acgaagccaa agaaatcgga gccgctatgg ttgcaatcaa
     1021 tacaacttgg acgaaattct gtaaagaaca attaaaagga acaaacgtac atgtaggagc
     1081 tgccattagc tttcctttag gacaagccgg tctagcatct aaaattgccg aaacaaagat
     1141 tgcgattgaa gatggagctg acgaaatcga ttatgtcatt gatattggac aagcaaaaat
     1201 gcaccactgg gactacatta aagaagaaat ggaaagtatt gtttctattt gtagagaaca
     1261 caacgtcatt tcaaaagtca tctttgaaaa ttgttattta gaaaaagatg aaatcaaaca
     1321 agttgcttta attgccaaag aagtaaaacc agacttcatt aaaacaagca caggctttgg
     1381 aactggtgga gccactttag aagatgtaaa attaatggtt gaaactgtcg aaggacaagt
     1441 aaaagtcaaa gctgcaggag gcattcgtga ttggaaaaca tgtaaagcca tgatcgatgc
     1501 aggtgcacaa agaattggaa caagctcatc cctaaaaatc ttagaagaat ttgaagctga
     1561 ggaaaagtaa catgaaacct tttcttttag aacccattcc tgattataca atttggggaa
     1621 ccaaccacat ctctaaagct agaggcatcg atgaagacta tgggacttgg tgggaagtca
     1681 gtgcccaccc ttattgttca aacaaaattg ttggaacgga taaaacattg atggaaatga
     1741 ttcaagaaaa tccggatgaa gtattaggta aaggatatac gcttcatgaa acattacgcc
     1801 tttcttatct agatacaaag gatgctttaa gcattcaagt tcacccagaa gatgactatg
     1861 ctttagaaca ttcaaatgat tatggaaaat atgaaagctg gtatattcta gatgctaaac
     1921 caggcgctac gctagttgcc ggaacaacaa tcaatgatgc agatgaaatc aaaaatgcct
     1981 taatgaacaa cgatgttgaa aaatatctaa ataaagtcga agtacataaa ggagactata
     2041 tcatcattcc tagtggtatg cttcatgcct taggaaaaga catcttagct ttagaagtag
     2101 gtacaaactc gaacacaacc tatcgtttct atgactacaa ccgtaaggga aaagatggaa
     2161 aaacacgtcc tttacatgtc aaagaatctt ttgatgtcac aaacttcaat ttaaaaccta
     2221 tctttgttcc acaaaaacag gaaacacacc gaattggcaa ttgccctaac tttacagtgg
     2281 atgaagttta tgctgattct gatattacct taacatgtga agaacattac atgattctat
     2341 ccaatattga ttcagattgt acgattgtat ggaacagtga agaaattgta ttaccacaat
     2401 atgacagtct atttgttcca ttcgcatcca agaaaatcac aatcaaacaa ggagcacacc
     2461 tattagtgtc aaatcctaga aaggactaat ttatgaaaac cattttagtt actggagcca
     2521 atggctatat cgcaagctta gtaagagcgt atcaaaaaga taactttaac tggatctgca
     2581 tgacaagaaa ggatgcagac cttacaaatc ctgaagacgt aaaaagattt gtagcttctc
     2641 aagattttga catttgtttt cacacagccg ccaatgcaac tacagccttc tgcaatgaac
     2701 atccagattt agttcataaa atcaatgtag aaagtaccca agctatcatt gattgttgta
     2761 aagaaaaagg tgctaaattg attttctgtt caaccgaaca agccttcaat ggaaaagaaa
     2821 atcacggtcc ttttaaagaa gacgaaccat taagtgctgt gactgtatat ggacaaaata
     2881 aaatcgaatg tgaagaattg attcaaaaac aattggatga tgcgattatc ttacgcttta
     2941 catggatgat gggactaagc tatccaggca tcaaggcaag tccaagtatc attaaaaatg
     3001 ttatgaatgc gctaatcaac catactccaa cattatttac tgtgaatgaa aaacgtggta
     3061 tgacatatgc caagcatttg gcaacacaat ttgataaaat cacagaactt gaaccaggca
     3121 tttatcatgt ggcatccaaa aacactatga ccacatatga atctgcaaaa tatgtggcta
     3181 aagctctagg tgcaagtgat gaactcattg aagaaatcat tttgccaaac aacgaacgat
     3241 accaagatcg attcagagat tatcgtttag atagtgaaaa actagaaagt cttggaattc
     3301 attttgcgac atttgaagaa gacgtaaatg aaattctaat ggattttgga tggaagaaaa
     3361 gttaaaatag cttgtaggag gctatttatg actatacttg aggaaaatgt taaaaaatat
     3421 taccaaagcg gatacaattg ttcagaaacg ctacttcatg cctgcaatga aacctatcaa
     3481 ttagatattt cagaagaaga catgaaaatg atggctggat ttggtggcgg catgtttatt
     3541 ggaagtacat gcggcgcatt agtcggatcg attgctgccc tatcaaaaat agtatgtaag
     3601 acaaaagcac atgaaatgtt accagaatta agacctttga ttcaaaaaca tacacgtaac
     3661 tttaaagaac tattaggcaa tttagattgt gctcacatca aacctgtaca tcatagtacg
     3721 gatccaaaca ttaaatgtat gaacacttgt ttattagcgg caaaagctct agaaaaaaca
     3781 atggaggaag aacacctatt ataggattct tcctcttttc ttatattcgt aataacctgt
     3841 ctactcgatg aatcaaatct tgttgaatac taaaatcaac ctcaatacca tcctctttat
     3901 attcctgctt atgcacctta ccattggtat gaatatagtc aagtacactt accttttcat
     3961 aaggaatcac gacatgtaaa gtttccatat gaaataattc cttttcaatt tcctgcttta
     4021 aaaattcaat actcgacttt tccttagcac aaatataaat acgattctct acacgttttg
     4081 gatattcgat atttgtcttg tcacacttgt taaagacata caaatatgga actttctcac
     4141 aaccaatttc ttttaatgtt tcttcacaaa cctgaagttt actatcctta tgttcatctg
     4201 aaatatccac gacttttaaa atcaaatccg aaacaacagc ttcttcaagt gtagaatgaa
     4261 aggcttgtac taaactgtgt ggtaaatcac taataaatcc caccgtatca cttaaagtaa
     4321 atacattatg attctccgtt tcaatttgac gtgttgaagt tgtcaaagtc gcaaataaca
     4381 tatccttctc aaataccgac ttctcttcat tcacaaatcc attcataata ctcgatttgc
     4441 ctgcattggt ataacctgtt agtgctacac ttttaaccat acctttttta cgctgatttc
     4501 tctgtacact tctttgcgta tggatcttat ccaattcttt ttcgacaaaa tgaatcttac
     4561 tctcaattct tcgcgcatcc agttcgattt tcttttctcc tgttccctta ttttttgaac
     4621 caccacgctg cctatccata tgtttgtagc ttccttgaag tcttggcaat aaatatttaa
     4681 gtcttgcaca ttcaacctga agcatactct cttttgtctt agcacgagaa gcaaagattt
     4741 ctaaaatcaa agcactctta tccatgacct cacaatcaaa aaagtccgat agatttcttt
     4801 gtacagaagg cgaaatagcc tctaatacaa gaaccatact acatgcttga tcttcaagaa
     4861 tgcgtttgat ttcttcaatc ttaccaggat tcatataata ttttttatga ggatgctcca
     4921 aattttgtga tacactcatg caaatttcaa tatcacaggc aagagctaat tctttcattt
     4981 cctccattct ttcttcaaaa gaatggcttg tgtatgtttg tacaccaatc aatattgctt
     5041 tttccataaa tgtattttat catacgatgc taaaatgtta taatatttca tgaggtgaat
     5101 tccatgaaaa ttgcttattt aatgcactat atgaaaaata attttgaaga aataacagaa
     5161 aacctagata tattgatcaa ccaaggcgat gatgtttact tgatggtaaa cgatgatgat
     5221 ctacgtgatg atttgattct atcctattgt gatacaccac agttacatgt ggctcaccac
     5281 caacaaactg cccttccagc cgatatgtca ctacctagag gctttattgt attgatgaaa
     5341 gatgcgttgg aatatgaaaa agaagacggt tcctttactt atgatcgttt catcataatg
     5401 acagatggta tgatccctac aaaatcaaaa gcacaacttg tagaatattt agaagcacat
     5461 gaagacgaag atatctatta tgaagtggat aattccaagg ataatgaaga agtaaagaaa
     5521 agatttgaaa actatgcctt ctttacaaac tcttatgatt tccaaaaatc aaaaaccatt
     5581 cgtgccatga ataaaatgac atccagtctt gttcattcct ttaaaaaacg tgaaattgaa
     5641 gacacaatct atgtcagcta tccttggttt gtactaacgc atgaaagtgc aaaagccttg
     5701 gcagacaatt tagcctattg ttcaaataca tttaaaatgt gtttgtaccc agaagaaatg
     5761 gccattgcga caatgttaca taaattctca aatgtagatc atcacaatga aaacatttgg
     5821 gcaacaggaa aagatggaga atacaaactt caaaagccaa tggaaaatgt agaattagac
     5881 gcaatcaaag agtctactta ttttgcagct ctagctcata gtgaagataa tcttcaagtt
     5941 tatgaagatt attttgatgc ctacacatgc gaatcataat cgtttcggat aatcatggca
     6001 atccggatat cctaaaaatt ctttatcaca tctattcttc gctctataaa gacaatgtga
     6061 aattcattca tttaggagat tctcaagcaa caaatatcaa tgatttaaat aactttatat
     6121 gtgtcaaagg aaataatgat acgttagatt tacctaaatt tcaaagatta ac
//
LOCUS       DBMDWL010000123         6145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_4986_length_6145_cov_1.854070, whole genome shotgun sequence.
ACCESSION   DBMDWL010000123 DBMDWL010000000
VERSION     DBMDWL010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4986_length_6145_cov_1.854070"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="ACZPDN_08480"
     CDS             <1..523
                     /locus_tag="ACZPDN_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1298701.1"
                     /translation="DDTKKLVDQFQTVVDESEAWYDRNRTTIVNSDRLYFLGYGIDVA
                     TAIEAQLKVGEMLRLPALGYEIEEYSHGPTMAISKKQTICIIGSDEAEFEHSVDFSKS
                     FKKYTDRVHFITCKDLGNDPRDCVFSVKTNKYLAPLMFTVPFQFVAAKGAKDIGIDTA
                     INPFNENLAHYKD"
     gene            523..1155
                     /locus_tag="ACZPDN_08485"
     CDS             523..1155
                     /locus_tag="ACZPDN_08485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1298702.1"
                     /translation="MKAIIFDMDGVLINSEVKYQELFMQFFKDRGVFIEKEELLFLVG
                     CSRKTEDEFIASRLDISVERAQDLKNNFFENHKVDYLKIRMPYVLELLSYLKNKQITM
                     ALASSSPMDNIEDVLKQCEIETYFKYIVSGENFKRTKPDPEIYEYTCKQIGVSKDEIW
                     VVEDSEYGIEAAKNAGLKVMGLYNSKLYQDLTDANLIIGSLKQIMEEDIW"
     gene            1149..1574
                     /locus_tag="ACZPDN_08490"
     CDS             1149..1574
                     /locus_tag="ACZPDN_08490"
                     /inference="COORDINATES: protein motif:HMM:NF015567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA
                     domain-containing protein"
                     /protein_id="HIR1298703.1"
                     /translation="MVGILITGHGHFASGIASSVELIMQSLEGVEVVDFPQGDTATEL
                     KENIHSALSKFDDEIVVFADLLSGSPFNTAVMEALENDKIQVVYGTNLGMLIECIMMR
                     NMGSSAIEIANKACEVGQTQIGKFEKQLESEDDSSDEEW"
     gene            1576..>1889
                     /locus_tag="ACZPDN_08495"
     CDS             1576..>1889
                     /locus_tag="ACZPDN_08495"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase family protein"
                     /protein_id="HIR1298704.1"
                     /translation="MTRSIMWQYIEEEPTYLLNILNNPENKDVIQKIKDFDTIYICAH
                     GSSYNAACSVAGFISDMAHVRVYVYTPSNFKHNAKSINYENKNKTLVIGISETGTSRG
                     VL"
     assembly_gap    1890..1899
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1900..2659
                     /locus_tag="ACZPDN_08500"
     CDS             <1900..2659
                     /locus_tag="ACZPDN_08500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1298705.1"
                     /translation="VLEVLEQMKDKGFTLLTLTNVKDSPMDTLGDYTLYYHCDEEDSN
                     AKTKGYSCTLLLLMLIGIYSGYFKGFVSNEEVKKYFEELKNEIKNLESLMDSFVSWLK
                     DASYGKDMKDIYVIGDGMQFGSCLEGQLKLMETMCMPTMFNDILEFSHGMHRSLNTYS
                     HVLLLDDGTEKELMYKTYQYLKAKKIPVLLITNQSYAYEDYIYPVDLYHVDHSIFSII
                     YLIQIISVFVPELHGLDPNRDANNDYTDFVNTRV"
     gene            2709..3362
                     /locus_tag="ACZPDN_08505"
     CDS             2709..3362
                     /locus_tag="ACZPDN_08505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1298706.1"
                     /translation="MLELVVFDVDGLMLDTESVWKEAFDQAGNKYGIQNMGSTLFPKL
                     IGKSGRDEKEVLDRYLSQDIQKLVIKEWERIGYGMLEKEVPVKPGLNEILDFLDAHHI
                     KKAVATTTRRELTEERLKRVGVYDRFDYVLCGDEVINRKPDPEIYLSVLKKMDTDARN
                     ALVLEDSVVGVEAAYRAGIDCIQVPDIIAPTDVQKKQTIYTAKDLIEAKDYIKENRV"
     gene            3364..4713
                     /locus_tag="ACZPDN_08510"
     CDS             3364..4713
                     /locus_tag="ACZPDN_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1298707.1"
                     /translation="MRQNDFENAPMIKLVMGLAIPSMIAQLVNILYSIVDRMFVGQIE
                     EVGNLCLAAVGICGPIVTLLSSFGTLVGIGGSIWLSMCLGQKNEKRASQILYNSFVML
                     VVLSICLTLGFILLKHNLIYWFGGSDALYPYANTYLTIYTLGTFFALMSIGLNYFITG
                     QGYATIAMLSVCIGAITNIVFDFILIRTLKIGVAGAAIGTVVAQFISCMFVLCFLRFK
                     SKIKLKKEELSIEKMKHIVKLGFSPFLILASDSVILIVMNMMLQKYGGNMGDVYISAI
                     TVAQSYFLLITGPLLGISSGTQPIYAYHFGSQNLKKIKEAFKIVISFAFLFCLIMFII
                     SMCYSNVFVAMFTNDAMTMPIADRAIKIFCLGILMMSIQYVLVDGITGLSNVKLSLFL
                     SLNRKMMYLGCTCLLPAFLGVSNIFYAQPAADIYASIVTIVCFIKIIPSYLKSHGVK"
     gene            4793..>5146
                     /locus_tag="ACZPDN_08515"
     CDS             4793..>5146
                     /locus_tag="ACZPDN_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1298708.1"
                     /translation="MAIFLNPDNANFNEAVHSKIYVDKTGLIEYTNSVLSTTAKFICN
                     SRPRRFGKSMTADMLCAYYSKGCDSRELFQPYTISSCSTFEKYINQYDVIHIDVQWCK
                     DQVENINDIVNYIKES"
     assembly_gap    5147..5156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5157..>5551
                     /locus_tag="ACZPDN_08520"
     CDS             <5157..>5551
                     /locus_tag="ACZPDN_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1298709.1"
                     /translation="IDYNGIISLSGTLSKINDETGHRFVVIIDEWDVLIRDNANNKKL
                     LEEYIDFLKGLFKGSQPSRYIALAYLTGILPIKRTMTQSALNNFDEYTMLNPGPLASF
                     IGFTEGEVKVLSNKYNIDFERIKKWYDGY"
     assembly_gap    5552..5561
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5562..>6145
                     /locus_tag="ACZPDN_08525"
     CDS             <5562..>6145
                     /locus_tag="ACZPDN_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1298710.1"
                     /translation="GYYLNHQHVYNPKAVVSLFTLGVFQSYWAQTSSYESIHSLIQMN
                     FDGLKEAVLEMLSGNEVKMQTTSFQNDMVSFKNMDDVLTALVHLGYLGYNQTYKTVFI
                     PNEEIRQEFVDSVSEDKWNDLIQFERESDTILEATCQIKTEVVAKQMEMIHNTYASNI
                     AYHNENSLSSVLTIAYLSTLKYYFKPIRELPTGI"
BASE COUNT         2078 a          741 c         1218 g         2078 t
ORIGIN      
        1 ggatgatact aagaaattgg tagatcaatt ccaaactgta gttgatgaaa gtgaagcttg
       61 gtatgatcgt aaccgcacaa caatcgtaaa tagtgatcgt ttatatttct taggttatgg
      121 tattgatgtt gcaaccgcaa ttgaagctca attaaaagtt ggagaaatgt taagacttcc
      181 tgcattggga tatgaaattg aagaatattc tcatggtcct acaatggcga tttctaaaaa
      241 acaaaccatt tgtattattg gaagtgatga agctgaattt gaacacagtg tagacttcag
      301 taaatccttt aaaaaataca cagaccgtgt tcactttatc acatgcaaag atttaggaaa
      361 cgatcctaga gattgtgtat tctctgttaa gacaaataaa tatttggctc cattgatgtt
      421 tacagttcca ttccaatttg ttgccgcaaa aggtgcaaag gatattggta ttgatacagc
      481 tatcaaccca tttaacgaaa atttagctca ctacaaagac taatgaaagc tattattttt
      541 gatatggatg gtgtcctaat aaatagtgaa gtgaagtatc aagaattatt tatgcagttt
      601 tttaaagata gaggagtttt tattgaaaaa gaagaacttc tctttcttgt tggttgttct
      661 agaaagacgg aagacgaatt tatcgcaagt cgattagaca taagtgtaga aagggcacag
      721 gatttaaaga ataacttttt tgaaaatcat aaagtggatt atttaaaaat acgcatgcct
      781 tatgtattgg aattactttc atatttaaag aacaaacaaa ttacgatggc gcttgcttca
      841 tcttctccaa tggacaatat tgaagatgtt ttaaaacaat gtgagattga aacctatttt
      901 aaatatattg tgagtggaga aaattttaaa aggacaaaac cagatccaga aatttatgaa
      961 tatacatgta aacaaatcgg tgtttcaaaa gatgaaatat gggttgtaga agattcggag
     1021 tatggaatag aagctgcaaa aaatgcagga ttaaaagtaa tggggttata caatagtaaa
     1081 ctttatcaag atttgacaga cgcaaattta attataggtt cactaaaaca gattatggag
     1141 gaagatatat ggtaggaatt cttattacag gtcatggtca ttttgccagt ggcattgctt
     1201 caagtgtaga gttgatcatg caaagtctag aaggggtaga agttgttgac tttccacaag
     1261 gagatacggc aacggaatta aaagaaaata tacatagcgc attaagtaaa tttgatgatg
     1321 aaattgtcgt atttgcggat ttactaagtg gatctccatt taatacggct gttatggaag
     1381 ctttggaaaa tgataagatt caagttgtct acggcacaaa tctaggtatg ttgattgaat
     1441 gtatcatgat gcgtaatatg ggttcaagtg caattgaaat tgcaaataag gcttgtgaag
     1501 ttggccaaac tcaaattggt aaatttgaaa agcagctaga atctgaagat gattcatcgg
     1561 atgaggagtg gtaaaatgac tcgaagcata atgtggcagt atatagaaga agagcctaca
     1621 tatttgttga atatcttaaa taatccagag aataaagatg ttattcagaa aataaaggat
     1681 tttgatacca tttatatatg tgcacatgga agttcttata atgcagcttg ttcagttgct
     1741 ggttttataa gtgatatggc tcatgttcgt gtatatgtct atacacctag taattttaag
     1801 cataatgcca aatccatcaa ttatgaaaat aaaaataaga cattggtgat tggaattagt
     1861 gaaacaggta cgagtcgagg tgttttagan nnnnnnnnnt gttttagaag ttttagaaca
     1921 aatgaaagat aaaggtttta cacttctaac acttacaaac gtaaaagatt cacctatgga
     1981 tacattagga gattacactc tttattatca ttgtgatgaa gaagatagta atgccaagac
     2041 aaaagggtat agttgtactt tattattatt gatgttgatt ggtatatatt caggttattt
     2101 caagggattt gtttcaaatg aagaagtgaa gaaatatttt gaagagttaa aaaatgaaat
     2161 caagaatctt gaatcattaa tggatagttt tgtatcttgg cttaaagatg caagttatgg
     2221 aaaagatatg aaagatatct atgtgatcgg tgatggaatg caatttggtt cttgtttaga
     2281 aggccagctt aaactaatgg aaacaatgtg tatgccaacc atgtttaatg atattttaga
     2341 attttcacat ggtatgcaca gatctttaaa tacatattca catgtattgt tgttggatga
     2401 tggtacggaa aaggaattga tgtacaaaac atatcaatat cttaaagcta aaaaaattcc
     2461 agttttattg atcacaaatc aaagttatgc atatgaggat tatatttatc ctgttgattt
     2521 atatcatgtg gatcattcaa tattttctat catttacttg attcaaatca tatctgtatt
     2581 tgtacctgaa cttcatggtt tagatccaaa tcgtgatgcg aataatgact atacagattt
     2641 tgtgaatact agagtataat taaaaagtaa agcccagatg ggcttttcgc atatagggga
     2701 ggatgcacat gttagagtta gttgtttttg atgttgatgg gttgatgtta gatactgaaa
     2761 gtgtatggaa agaagctttt gatcaggcgg gtaataagta tggtattcaa aatatgggtt
     2821 ctactttatt tccaaaattg attggtaaaa gtggtagaga tgaaaaagaa gtgttagatc
     2881 gatatttatc tcaagacatt caaaaattgg taattaaaga atgggaacgc attggatatg
     2941 gtatgttgga aaaggaagtg ccagtaaagc caggtttaaa tgaaatccta gattttctag
     3001 atgcacatca tattaaaaag gctgtcgcaa ctacgacaag aagagagctt actgaagaaa
     3061 gattaaaacg agttggcgta tatgatcgtt ttgattatgt attatgtggg gatgaagtca
     3121 taaatcgtaa accagatcca gaaatttatc taagtgtttt aaagaaaatg gatacagacg
     3181 caagaaatgc tcttgtttta gaggatagtg tagttggtgt tgaagctgct tatagagcag
     3241 gtattgattg tattcaagta ccagatatta ttgcgcctac agatgtgcaa aagaagcaga
     3301 cgatttatac tgcgaaagat ttgatcgaag ctaaagatta tattaaagag aatagggtat
     3361 aaaatgcgtc aaaatgattt tgaaaatgca cctatgataa aacttgtcat gggtttagct
     3421 ataccgagta tgattgctca gttagtcaat attttatatt ccattgtcga tcgtatgttt
     3481 gtaggacaaa ttgaagaagt agggaattta tgtttggcag ccgttggaat atgcgggcca
     3541 atcgttactt tattatccag ttttggaacc cttgttggta ttggtggttc aatttggcta
     3601 tcgatgtgtt tgggtcaaaa gaatgagaaa agggccagtc aaatcttata taacagtttt
     3661 gtgatgttgg ttgttctttc catttgttta acacttggat tcattctttt aaaacacaat
     3721 ttgatctatt ggtttggagg cagtgatgcc ctttatcctt atgccaatac ctatttaaca
     3781 atttacacat taggaacttt ctttgcccta atgtcaattg gattgaatta ttttattacg
     3841 ggacagggct atgccacgat tgcgatgtta agtgtatgta ttggtgccat cacaaatatt
     3901 gtttttgact ttattttgat tcgtacactt aaaattggtg ttgccggagc tgccattggt
     3961 acagttgtag cccaatttat atcgtgtatg tttgtgcttt gttttttaag attcaaatca
     4021 aaaatcaaat tgaaaaaaga agaactatcc atagagaaga tgaagcatat agtaaaactt
     4081 ggcttttcac catttttgat tcttgcatcc gattccgtta ttttaatagt gatgaatatg
     4141 atgcttcaaa agtatggtgg aaatatgggt gatgtttata tttcagccat tactgtagcc
     4201 caaagctatt tcttgttgat tacaggtcca cttttaggta ttagttcagg aacacagcca
     4261 atatatgcgt atcattttgg atcgcaaaat ttaaagaaga tcaaagaagc ctttaaaatt
     4321 gtgatttcat ttgcgttttt attttgtttg atcatgttca ttatttcgat gtgctattcc
     4381 aatgtatttg tggctatgtt tactaatgat gccatgacta tgccaatcgc agatagagct
     4441 attaaaatct tctgtttagg tattttaatg atgtccattc aatatgtttt agtggatgga
     4501 attacaggat tatctaatgt aaaattatcg ttatttttat cattaaatcg taagatgatg
     4561 tatttgggtt gtacatgttt attgcctgct tttttaggtg tttctaatat tttctatgca
     4621 caacctgcag cggatattta tgcaagtatt gtgactattg tttgttttat aaaaatcata
     4681 ccttcttatt taaaaagtca tggagtgaag taaggcgaga tgaactaaca tctcgttttt
     4741 ttttaatagt atagtctttt tctgatataa tagtggtgta ggaggaatct gtatggctat
     4801 atttttaaat cctgataatg cgaattttaa cgaagcggtt cattcaaaaa tatatgtgga
     4861 taaaacgggt ttaattgaat atacaaatag tgtgctttca accacagcta aattcatttg
     4921 taattcaaga cctaggcgtt ttggaaaatc tatgactgcc gatatgcttt gtgcttatta
     4981 ttcaaaggga tgtgattcta gagaattgtt tcagccatat acgatttctt cgtgttctac
     5041 atttgagaag tatataaatc aatatgatgt cattcatatt gatgtacagt ggtgtaaaga
     5101 tcaagttgaa aatataaatg atattgtaaa ttatatcaaa gagagtnnnn nnnnnnatat
     5161 tgactataat ggaattattt ctttgtctgg aacactttct aaaatcaatg atgaaacagg
     5221 acatcgcttt gtggtaatca ttgatgagtg ggatgtattg attcgtgata atgcgaataa
     5281 taaaaaatta ctagaagaat atattgattt cttaaaagga ttatttaaag gatcacaacc
     5341 ttctcgttat atcgcattag cgtatttaac aggtatttta ccaatcaaaa gaacgatgac
     5401 acagtccgca ttgaataatt ttgatgaata tacaatgttg aatccaggtc cattggcttc
     5461 ttttatcgga tttacagagg gggaagtaaa agtattaagt aataaataca atatagattt
     5521 tgagagaatt aaaaaatggt atgatggata tnnnnnnnnn ntggatatta cttaaatcat
     5581 cagcatgtat ataatccgaa agccgttgtg agtttattta ctttaggtgt gtttcaaagt
     5641 tattgggcac agacaagcag ctatgaatcg attcatagtt tgattcaaat gaattttgat
     5701 ggcttaaaag aagctgtttt agaaatgtta agtggtaatg aagtgaagat gcagacaacg
     5761 tcttttcaaa atgatatggt ttcatttaaa aatatggatg atgtcttaac ggcattggtt
     5821 catttaggat atttgggata caatcaaaca tataagacgg tatttattcc aaatgaagaa
     5881 attcgacaag aatttgtgga ttctgtttca gaagataaat ggaatgattt gattcaattt
     5941 gaaagggaat cggataccat tttagaagca acttgtcaaa taaaaacaga agttgtagct
     6001 aaacaaatgg aaatgattca taatacgtat gcttctaata ttgcatatca taatgaaaat
     6061 agtttgagca gtgttttaac gattgcgtat ttatcaactt tgaaatatta ttttaaacca
     6121 atacgagaat tgccaacagg aatag
//
LOCUS       DBMDWL010000124         6050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5112_length_6050_cov_2.179526, whole genome shotgun sequence.
ACCESSION   DBMDWL010000124 DBMDWL010000000
VERSION     DBMDWL010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5112_length_6050_cov_2.179526"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..63)
                     /locus_tag="ACZPDN_08530"
     CDS             complement(<1..63)
                     /locus_tag="ACZPDN_08530"
                     /inference="COORDINATES: protein motif:HMM:NF023742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1298711.1"
                     /translation="MEKAFKYRIYPNREQRILLAK"
     gene            complement(87..488)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_08535"
     CDS             complement(87..488)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIR1298712.1"
                     /translation="MEVVKGRGYVYSIQYHIVWCVKYRRNILNGQVATRLKELLTQIS
                     KDNGFQILEMNIEEDYIRMLINCTPQHYIPNIIKAMKGVSARLLMKEFGDDLRSKLWG
                     GHLWNPSYFVATVSENTEEQIIEYIRSQKRK"
     gene            complement(844..1233)
                     /locus_tag="ACZPDN_08540"
     CDS             complement(844..1233)
                     /locus_tag="ACZPDN_08540"
                     /inference="COORDINATES: protein motif:HMM:NF020438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HIR1298713.1"
                     /translation="MDERWYTMDRPKSKYPKMSMLDRAAQFAPFAALTGHKEAVIEQG
                     RITQEKRILSNEEKLEINEKITELMHLKSKCRITYFEKDKTKSGGQYMESVLSFKRID
                     ELNKVLYFKENIQIQIDDIVDIEILEQ"
     gene            complement(1233..2669)
                     /locus_tag="ACZPDN_08545"
     CDS             complement(1233..2669)
                     /locus_tag="ACZPDN_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1298714.1"
                     /translation="MKSKIYLAIDLKSFYASCECVSRGLDPLTTNLVVADASRTEKTI
                     CLAVSPSLKQYGIGGRCRLFEVNQKVRDVNAKRKKGFLHFDGKSSDDEILKQNPRFEV
                     DFLIAPPRMKYYLDISTQIYNIYLKYVSSQDIHVYSIDEVFIDATPYLNTYQLTPKEF
                     AMKMILDIYETTGITATAGIGTNLYLAKVAMDIVAKHKDPDENGVRVAALNEYRYRKL
                     LWNHKPLTDFWRVGKGYEKRLNKLGIDTMGKLCAFSLEHEDVLFKTFGVNAELLIDHA
                     WGYESCTMEDIKAYKPEKNSMGSGQVLSCGYEKEKAKILVKETVESLALSLVDKHLVT
                     NKVVLTIGYETNEAYTGETMVDFYGRVLPKSLNKIVHLPQKTNANSILIQNILDVYDR
                     YVDSSLLVRRINVAAVEVVDEKNRGFEQIDLFSNADEEELKKENDLQKVTLKIKNKYG
                     KNSILKGTDFEKGAKAKERNEQIGGHKA"
     gene            complement(2666..2830)
                     /locus_tag="ACZPDN_08550"
     CDS             complement(2666..2830)
                     /locus_tag="ACZPDN_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298715.1"
                     /translation="MNIINMVKKYQKKKAKKAAKEELKQMIPTLRSALNEVTSGLDEL
                     NGLLKKITRS"
     gene            complement(2833..3312)
                     /locus_tag="ACZPDN_08555"
     CDS             complement(2833..3312)
                     /locus_tag="ACZPDN_08555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1298716.1"
                     /translation="MIRKGTIEDAEILSKVEATCFPPNEAATSKEIQERLNVYANHFL
                     LLFEKNQLISFIDGFCTNQRDLTVEMYSDASMHDENGDWQMIFGLNTLPEYQRKGYAT
                     KIMNAMIDLARQEKRKGLVLTCKDRLVPYYSKFGFKNEGVSSSVHGGVVWYQMRLEF"
     gene            complement(3352..5919)
                     /locus_tag="ACZPDN_08560"
     CDS             complement(3352..5919)
                     /locus_tag="ACZPDN_08560"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR1298717.1"
                     /translation="MLQCKNIMKDYVSGDEIVHALKGVSLSFREHEFVSILGQSGCGK
                     TTFLNIIGGLDHYTSGDLIINGKSTKDYSDKDWDTYRNHQVGFVFQSYNLIMHQSVLS
                     NVELALTLTGVNKEERRKRAIEALNKVGLSDQIHKKPTQMSGGQMQRVAIARAIVNNP
                     DIILADEPTGALDSTTSVQIMEILKEISKDKLVIMVTHNPQLADEYSSRIIRLKDGTL
                     VSDSNPFNEQEMNVDTSVLKRPGMSFKMACSLSLNNLMTKKARTFLTSFAGSIGIIGI
                     ALIMSLSHGMQSYIDQMENDTMASYPIEIQSSSSDMSTLMTTMMGMKKKTEEHKDSKI
                     YSRPYVEDVLESLSSSKKNNLSAFKSYIESSKGKEFRKTAKAIEYDYNLNLQVYNENT
                     DSGLVQVSPNGLLDKLGMSDMMSIQSQFMDSSAMTNDQVWLSLPESKKLRDDEYQLVE
                     GKWPTNYNEVVLEVDENNEITDYALYSLGLLDQDELVKNYQKILNGETDKISKTNLKS
                     YSVDDILNLKFRLVLNSDLYQKVNGLWINQSENESYMQDVVSKSPEIKVVGIIKPSES
                     TVSQPTMGGVYYTKAMEEYVTSKTENSQIVIEQKTNPNINIFTQAEFASGQKMSMSNL
                     TNEQMMQLSSMSQEELMNYMNTYNENINATYDSNLTKLGVVDYSNPTKISLYASSFDG
                     KEKLGDLITSYNKKQTKSNVITYNDFIGTMLSSVTSVVNIISYVLIAFVSVSLIVSSI
                     MIGIITYISVLERTKEIGILRSIGASKKDITRVFNAETFLIGLISGVLGILITLVLNV
                     PISVVVKNMTGVAHIAKLPVNGAVFLIFIDLVLTILAGLIPSKIASKKDPVEALRSE"
BASE COUNT         1917 a         1293 c          729 g         2111 t
ORIGIN      
        1 cttggctaat aatattcttt gttctctatt tggatagata cgatacttga atgccttttc
       61 catgatgatt tctccttatt gattcgctat tttctttttt gacttcttat atactctata
      121 atctgttctt ctgtattttc agaaactgta gctacaaaat aagaaggatt ccatagatgt
      181 ccaccccata acttggatct tagatcatcc ccaaactctt tcatcaagag tctggctgat
      241 acacccttca tagcttttat aatatttgga atgtaatgct gcggcgtaca attgatcagc
      301 atacggatgt aatcttcctc tatattcatt tccagaattt gaaaaccatt atcctttgaa
      361 atctgagtca gcagctcttt caatcttgtt gcgacctgtc cattcaagat attgcgacga
      421 tactttacac accatacaat atgatactgt attgaataga catagcctct acctttgaca
      481 acttccatat gtttttacct catcaatatt ataacatatg tatatattta aagcactata
      541 cttatcattt atttttaaat tatttatcta acatctgaac atctaattca tcgcatgact
      601 aaagtcacga gtgttcttag attgtactat acataaatgt attcagaata ttttctatct
      661 gatactcatg ttcgatatcc cataataaaa gatatcatct ttccactaac aagtattcta
      721 tttaaaacga ttgaaaggca taaacttcac aagacaaaaa tacaagcgtt ttaaattagg
      781 aatgcaatct ctatcaagca gccaatggct gttttttttc gtatttccaa agaaaaagga
      841 acatcactgt tccaatattt caatatctac aatatcatct atttgtattt gaatattctc
      901 tttaaaatat aacaccttat tcaattcatc tatacgctta aaagataaaa cgctctccat
      961 gtactgacca ccactttttg tcttatcctt ttcaaaataa gtgattctac atttcgattt
     1021 caaatgcatc aattcagtga ttttctcatt gatttcaagc ttttcttcat ttgataaaat
     1081 tcttttttcc tgcgtgattc taccctgttc tatcacagct tctttatgac ctgttaaagc
     1141 tgcaaaaggt gcaaattgag cagcacgatc taacatcgac atctttggat atttactttt
     1201 aggacgatcc attgtatacc atctttcatc cattatgcct tatgccctcc aatctgctca
     1261 tttctttctt tggccttggc acccttctca aaatccgttc cctttaaaat tgaatttttg
     1321 ccatacttat tctttatttt taaagtcacc ttttgaagat cattttcctt cttcaattct
     1381 tcttcatccg catttgaaaa taaatcgatc tgctcaaagc cacgattctt ttcatccacc
     1441 acttctactg ctgccacatt gattcttcgc accaataaac tagaatccac ataccgatca
     1501 tatacatcca atatattttg aattagaatt gaattcgcat ttgtcttttg aggtaaatga
     1561 acaattttat ttaagctttt cggcaaaaca cgtccataaa aatcaaccat tgtctcaccc
     1621 gtataggctt cgtttgtttc ataaccaatt gtaagaacca ctttatttgt caccaaatgc
     1681 ttatccacca aacttaaagc taaagattcc accgtttcct ttactaaaat cttagccttt
     1741 tctttttcat atccacaaga taagacctga cccgaaccca tactattctt ttccggttta
     1801 tacgccttaa tatcttccat tgtacaagat tcatatcccc aggcatgatc aatcaatagt
     1861 tcggcattta caccaaatgt tttaaacaaa acatcctcat gttctaagct aaaggcacaa
     1921 agcttaccca ttgtatcaat acctaattta tttaatcttt tctcataccc ttttccaaca
     1981 cgccaaaaat cagtcaaagg cttatgattc cacaacaatt tacgatacct atactcattt
     2041 aaagccgcaa cacgaacccc attttcatct ggatctttat gtttggccac aatatccatt
     2101 gccaccttgg caagatataa attcgtacca atacctgccg tagccgtgat gccagttgtt
     2161 tcataaatat ctaaaatcat tttcattgcg aattcttttg gtgttaattg atacgtattt
     2221 aaatatggcg ttgcatcaat aaacacttca tcaatggaat atacatgaat atcctgactt
     2281 gaaacatatt tcaaatagat attataaatt tgtgtactga tatccaaata gtatttcatt
     2341 cttggaggtg caattaaaaa atcgacttca aatctaggat tctgctttaa aatttcatca
     2401 tcactcgatt taccatcaaa atgcaaaaac ccctttttcc ttttggcatt cacgtctctt
     2461 accttttgat tcacctcaaa caaacgacac cttccaccaa ttccatactg tttcaaagaa
     2521 ggcgatacag ccaaacatat tgtcttctca gttcttgaag catccgcaac aactaaattt
     2581 gtagttaaag gatccaatcc tctagaaaca cattcacaag aagcatagaa agactttaag
     2641 tcgattgcca aataaatctt tgacttcatg aacgcgtaat ctttttaaga agaccattta
     2701 attcatccaa accagaggtc acttcattca atgcacttct taacgtagga atcatctgtt
     2761 tcaactcttc ttttgcggct tttttcgctt ttttcttttg atatttcttc accatattta
     2821 ttatattcat atttaaaact ccaatcgcat ctgataccaa actacaccac catgaacact
     2881 tgaggataca ccctcatttt taaatccaaa cttactatag taaggcacca atctatcttt
     2941 acaagttaac actaaccctt ttcgtttttc ttgtctagcc aaatcaatca tcgcattcat
     3001 aatcttagtg gcataaccct ttctttgata ctccggcaaa gtatttaacc caaatatcat
     3061 ttgccagtcc ccattttcat catgcatcga agcatcacta tacatttcaa ccgttaaatc
     3121 tctttggttt gtacaaaaac catcgataaa actaatcaat tgattttttt cgaacaataa
     3181 aagaaaatga ttcgcatata catttaatct ttcttgaatc tcttttgaag ttgctgcttc
     3241 attgggcgga aaacaagttg cttctacctt agataatatt tctgcatctt caatcgttcc
     3301 ttttcgaatc atgaaatcac ctcaccctta attataccaa aaaagaagtg attactcact
     3361 tcttaatgcc tctacaggat cttttttact cgcaatcttg gatggaatca atccggcaag
     3421 aatcgttaat actaaatcaa tgaaaatcaa gaatacagct ccattgactg gtaatttggc
     3481 aatatgcgca acaccagtca tattctttac gaccacacta attggaacat tcaatactaa
     3541 agtaattaat atacctaata caccactaat caatccaata aggaatgttt ctgcattaaa
     3601 tacacgtgta atatctttct tactagcacc aatggagcgt aagataccaa tctcttttgt
     3661 acgttctaat actgaaatat acgtaatgat accaatcatg atgcttgata caataagcga
     3721 tacagataca aaggcaatca aaacataact tatgatattt acaacacttg ttactgaact
     3781 taacatcgtt ccaataaaat cattatatgt gatcacattg ctctttgtct gtttcttatt
     3841 ataagaagta atcaaatcac ccagcttttc ttttccatca aaacttgatg catataaaga
     3901 aatctttgtc ggatttgagt aatctacaac accaagttta gtcaaatttg aatcatatgt
     3961 cgcattgata ttctcattat acgtattcat gtaattcatc aattcttctt gcgacatact
     4021 tgaaagctgc atcatctgct catttgtaag atttgacatg ctcatctttt gacctgaagc
     4081 aaattcggcc tgtgtaaaaa tattgatatt tggatttgtc ttttgttcta ttacaatttg
     4141 agaattctcc gtcttagaag taacatactc ttccatagcc tttgtataat acacaccacc
     4201 cattgttggt tgtgaaaccg tggattcact tggcttaata attccaacca ccttaatttc
     4261 aggactctta gaaacaacat cctgcatata cgattcattt tcactttggt tgatccataa
     4321 accatttacc ttttgatata aatcgctatt tagaaccaat ctaaacttca aatttaagat
     4381 atcatccaca ctataagatt ttagatttgt ctttgaaatc ttgtccgtct ctccatttaa
     4441 aatcttttga taattcttaa ccaattcatc ctgatctaac aatcccaaag aataaagcgc
     4501 ataatcggta atctcattat tttcatcgac ctctaaaacc acctcattat agtttgttgg
     4561 ccacttacct tcgacaagct gatattcatc atctcgaagc tttttcgatt cgggtaaaga
     4621 aagccacact tgatcattgg tcatagcact tgaatccata aattgggatt gaatcgacat
     4681 catatccgac atgcctaatt tatccaacaa accatttggt gatacctgca ctaaaccaga
     4741 atctgtattc tcattataaa cctgaagatt taaattataa tcatactcaa ttgctttcgc
     4801 tgtctttcta aattctttac ccttactact ttcaatatac gatttaaaag ctgataaatt
     4861 attcttcttt gaacttgata aagattctaa tacatcctcc acataaggac gagaatagat
     4921 tttagaatcc ttatgttctt ctgtcttttt cttcatgccc atcatcgttg tcattaatgt
     4981 agacatatca ctcgaactcg attgaatctc aatcggataa ctggccattg tatcattttc
     5041 catttgatca atatacgact gcataccatg cgacaatgac atgatcaaag caattccaat
     5101 aatgccaatg cttccggcaa aagatgttaa aaatgtacgt gccttttttg tcattaaatt
     5161 atttaaactt aatgaacaag ccattttaaa agacatgcca ggtcttttta acacactcgt
     5221 atccacattc atttcttgtt cattaaaagg attggaatcc gatactaaag taccatcctt
     5281 taaacgtata attcgactcg aatattcatc tgccaattgc ggattatggg taaccataat
     5341 gacaagctta tcttttgaga tttcctttaa gatctccata atttgcacac ttgttgtact
     5401 atccaaagca ccggttggct catcggccaa aataatatct ggattattga caatagcacg
     5461 ggcaatggcc acacgctgca tttgtccgcc cgacatctgg gttggctttt tatgaatttg
     5521 atccgaaagt ccaaccttat ttaaagcctc tatagctctt tttcttcttt cctctttatt
     5581 gactcccgtt agtgttaaag ccaattctac attacttaaa acactttgat gcatgatcag
     5641 attataagat tgaaacacaa aaccaacctg atgattacga tatgtatccc agtctttatc
     5701 actataatcc tttgtacttt ttccattaat aattaaatcc cccgaagtat aatgatccaa
     5761 accaccaata atatttagaa atgtagtctt gccacatcca ctctgtccaa gtatggaaac
     5821 aaattcatgt tcacgaaaag aaagtgatac cccttttaaa gcatgtacaa tttcatctcc
     5881 tgaaacataa tctttcataa tatttttaca ttgaagcata aaaagccctc catttgaatt
     5941 aattataaaa aaatatctct tgatttttca atcgactgat tttaacatat tgttacgaaa
     6001 gcaacaggtg ttgattatga atagaaaaat acaaatgaca aaaagatgta
//
LOCUS       DBMDWL010000125         6009 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5159_length_6009_cov_2.195667, whole genome shotgun sequence.
ACCESSION   DBMDWL010000125 DBMDWL010000000
VERSION     DBMDWL010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5159_length_6009_cov_2.195667"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(143..352)
                     /locus_tag="ACZPDN_08565"
     CDS             complement(143..352)
                     /locus_tag="ACZPDN_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298718.1"
                     /translation="MILGIAWQLMGFLGSIAILCSATLHQWDYNGITGILGSLLGLDL
                     IIPFIVCITLFICGLVVCFKVIGEE"
     gene            complement(381..806)
                     /locus_tag="ACZPDN_08570"
     CDS             complement(381..806)
                     /locus_tag="ACZPDN_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298719.1"
                     /translation="MKSKRIIVVVVSSIVLCLCLFFIFQIEKDTVSPNKKYVSNKKDI
                     VHYTIKIYQEDKNKVYVYAESNSPIFENTNYRVDYNQKISKEDIQIKWMTLSGSTEPK
                     KNDQLGLANVKILKDGSVFNEKVISFVGKGVKAITDVIG"
     gene            complement(803..1504)
                     /locus_tag="ACZPDN_08575"
     CDS             complement(803..1504)
                     /locus_tag="ACZPDN_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR1298720.1"
                     /translation="MIDKYLKETCMLDYSNPEIQELIQKKKWKELDEFERIKEIYNFV
                     RDDILFGYNEDDAICASKITLDGYGQCNTKGTLFMALLRACQIPCRIHGFTIDKRLQK
                     GAMTGLVYRSAPQNIFHSWVEVYFENQWYELEGFILDQAYLKKLQNQFPNCKGAFCGY
                     GVAVKDFKNPTIDFNRNSTYIQSEGITQDFGVYDCPDDLLKEHHQQVSRLKGFAYRHI
                     GRHFMNRNVKKVRRR"
     gene            complement(1673..2437)
                     /locus_tag="ACZPDN_08580"
     CDS             complement(1673..2437)
                     /locus_tag="ACZPDN_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298721.1"
                     /translation="MVFKTIGNKSNPAILFFHAMGVTGESSMPVAKKLADKYYCVLPT
                     STVYCSNQKYLGKSDEIQQIKRFLSDHGIKEIELVVASSIGADLAMVFLTDTKIPVKH
                     IFFDGGQFAQIGKITRRIMVPFLYVAMKSLYWTNGKTLKKIMWCDDDNIKPYFIEAGK
                     NLSYTNLRKQMDDSLEDKSFPKLSEELQKHTFWEFGSREDHFKYRNAVMQAYKYGNFP
                     VFEGLNHMEYQIRYPEGFARMLEMIIKTNQLPELTF"
     gene            complement(2460..3113)
                     /locus_tag="ACZPDN_08585"
     CDS             complement(2460..3113)
                     /locus_tag="ACZPDN_08585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1298722.1"
                     /translation="MSDKIQNAYESSKNIYDDVLTQGNIFSKLYIKLFWSGTNDNDIA
                     QKVLSYIPNDFSGNLLDVPVGTAVFTQCKWIALKDAHITCLDYSMDMLKQAKKRFDGY
                     THIKCIQGDVSNLKMNAESYDIVVSMNGFHAFPNKKKAFQETWRVLKPGGTFIACFYI
                     KGKSKRTDWLVNHILSKKGWFTPPFQTEEELRNILKEMYKQIDLHIDGSMVYFCCTK"
     gene            complement(3103..4038)
                     /locus_tag="ACZPDN_08590"
     CDS             complement(3103..4038)
                     /locus_tag="ACZPDN_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HIR1298723.1"
                     /translation="MKKIHFEVHTDGFYGDYWKCKTDSDCAMIAMIGDDSQDYLARTS
                     VKWLHKLGLNVMTMSPAKKDYGHHNYPLERIENAIRWLKVHGNKKVGIVGASTTGTLA
                     LTAASYFEDITLTIGLTPSDFIWQGFMQGKKDGCKEWPIEGESLFSYREKPLLYMPFC
                     YKHPQYWHVIEQETKRTGDMINSRKLFDDSEAVHPILEEEKIKIEKIHGKLLLIGAED
                     DVLWDAAKYIRRMEEREHICKLEAVVYKYGTHFVFPESMLKMMLPIGSASFVKFAFQS
                     ARKHPKECLETRIDIDVRIRDAIEEWKKGEVGYVG"
     gene            complement(4044..4844)
                     /locus_tag="ACZPDN_08595"
     CDS             complement(4044..4844)
                     /locus_tag="ACZPDN_08595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1298724.1"
                     /translation="MKSKIDITGVSETMLQTLYARAKETNSQNAKIKDDIAVHIVENI
                     DYDFSKADKDSAMSSGVIARTIVLDRMVEQYLNVHTNAVVVNIACGLDTRCYRMKGKY
                     LRWYNLDLPQTMKIREQFLTDTRPMYQIAKSAMDDSYIHDIEYHGEDILVIIEGLTMY
                     LDEKEVLKMFSIIEKSFKKVTVLVETMSPFVVKHMKEKSIDQSNAKFLWGVKNRNELQ
                     KILPEFIFDKEVSLIEGMKELHPIYHLFGWIPLVRNISNKIIVLKNRS"
     gene            complement(4841..5617)
                     /locus_tag="ACZPDN_08600"
     CDS             complement(4841..5617)
                     /locus_tag="ACZPDN_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298725.1"
                     /translation="MKFYESGNQSKPVIFLFPGTCCLYSSFDHVLEGLHVYFYTVAVS
                     YDGFDPNENTQFYSMEEECEKIEQEIMDHYGGRIKAAYGCSLGGSFVSLLIQRKRIHM
                     DHGIIGSSDMDEAGKVMATIQASIVTPIMYKMVHTETLPKFMEKKIAQMDDSKKKLVN
                     GFLDMFGLGSPWISKCSIYNQFYSDLVTKVQHNMDVPNTKIHVFYATKMGKKYEKRYY
                     TYFKNPDIHRFNMQHEELFCCHANKWIEEVRNVVIGDVGL"
BASE COUNT         1922 a         1224 c          709 g         2154 t
ORIGIN      
        1 caaacatatt ataccaaaaa agaccgatat tcagattttt aaatcttgaa tattcggtcc
       61 ttttttatta agctgaaact tctttatccg ttacagctcc tattcccatc catagagatt
      121 tgtcaagtat ataaattgaa atttattctt cccctattac tttgaagcag actactaaac
      181 cacaaataaa tagtgttata caaacaatga atggaattat taagtctaat cccaacaaac
      241 tacctaaaat tccagtaatc ccgttataat cccattgatg aagtgttgct gagcacaaaa
      301 tagctattga gccaagaaac cccataagtt gccaagcaat gcctaatatc atttttttca
      361 cacaatcaca ctcccttatt ttatccaatt acatccgtaa tggccttaac acctttacct
      421 acaaaactaa tgactttctc attgaataca cttccatctt ttaatatttt gacattcgca
      481 agacctagtt gatcattctt tttaggttct gtactccctg aaagcgtcat ccatttaatt
      541 tgaatatctt ctttcgatat tttctgatta taatcaactc tataatttgt attctcaaaa
      601 ataggagaat tcgattcagc ataaacataa accttattct tatcctcttg ataaatttta
      661 attgtataat gcacaatatc ttttttatta gaaacatact tcttatttgg agatacagtg
      721 tctttttcaa tttgaaatat aaagaataag cataaacata aaactatact actaacaact
      781 acaacaatta ttctttttga cttcatcttc gacgtacctt ttttacatta cgattcatga
      841 aatgccttcc tatatgtcta taggcaaatc cttttagcct tgacacttgt tgatggtgtt
      901 cttttagtaa atcatccgga caatcatata caccaaaatc ttgtgtaatt ccttcacttt
      961 gaatatatgt agaattgcga ttaaaatcaa tggtaggatt tttaaaatct tttacggcta
     1021 caccatatcc acaaaatgca cctttacaat ttggaaattg attctgtaat ttcttaagat
     1081 atgcctgatc taaaataaat ccttcaagct cataccactg attctcaaaa taaacttcta
     1141 cccaactatg aaagatattt tggggtgcac tccgatacac caagcctgtc atggctcctt
     1201 tttgaagtct tttatcaatt gtgaaaccat gaattctaca tggaatctga caagctcgta
     1261 aaagagccat aaataaagtt ccttttgtat tacattgacc atatccatct aatgtaattt
     1321 ttgatgcaca tatcgcatca tcctcgttgt acccaaacaa aatatcgtct cgaacaaaat
     1381 tatatatttc cttaattctt tcaaattcat ccagttcctt ccacttcttc ttttgaatca
     1441 gttcttgtat ttcaggattg gaataatcaa gcatacatgt ttcttttaaa tatttatcta
     1501 tcatttttca cttatcctta acattctaat aattatgaac ctgcttatat ttatattctt
     1561 caaaatataa aagactttga aataggatct actaaagtgt ttttcttaac ttcatcattt
     1621 gaagcaagtc aacattaatc tctacaacaa tgcatttatc tgatcccttc tttcaaaatg
     1681 tcaactcagg taattgattt gtcttaataa tcatttctaa cattctggca aatccttctg
     1741 gataccgaat ttgatactcc atatggttca atccctcaaa aactggaaag ttcccatatt
     1801 tataggcttg catcactgca tttcgatatt taaaatgatc ttctctactt ccaaattccc
     1861 agaatgtatg tttttgtagc tcttctgata attttggaaa tgacttatct tctaaactat
     1921 catccatctg ttttctcaaa tttgtatatg acaaattctt tcctgcttct atgaaataag
     1981 gcttaatatt atcatcgtca caccacataa ttttcttcaa tgttttccca tttgtccaat
     2041 acaaactttt catggccaca tataaaaatg gaaccataat tctacgtgtt atcttaccaa
     2101 tctgtgcaaa ttgaccacca tcaaagaata tatgcttaac cggtattttt gtatctgtta
     2161 gaaataccat cgcaagatct gcaccaatcg aagatgctac cactagttca atctctttga
     2221 tcccatgatc acttaaaaat cgtttaatct gttgtatctc atcactttta cctagatact
     2281 tttgatttga acaatatact gtagaagtgg gtagaacaca ataatattta tccgctaatt
     2341 tcttggcaac tggcatactg ctttctccag ttacacccat cgcatgaaaa aaaagtatag
     2401 ctggatttga tttgttccct attgttttga ataccataat ctcaccttct atccatacat
     2461 tattttgtgc aacaaaaata taccatcgat ccatctatgt gaagatctat ttgtttgtac
     2521 atctctttta gaatatttct aagttcttcc tccgtttgaa atggtggggt gaaccatcct
     2581 ttcttagaaa gaatatgatt cacaagccaa tctgttcttt tagactttcc tttgatatag
     2641 aaacacgcta taaaggtacc acctggtttt aaaactcgcc acgtttcttg aaatgccttt
     2701 ttcttatttg gaaaagcatg aaatccattc atggaaacca caatatcata ggattcagca
     2761 ttcattttta aattggatac atccccctga atacatttaa tatgcgtata cccatcaaat
     2821 cttttcttag cttgtttaag catatccata ctataatcaa gacaagtaat atgcgcatct
     2881 tttaaagcta tccacttgca ttgagtaaat acagctgttc cgactggaac atctaaaaga
     2941 ttgcctgaaa aatcatttgg aatatatgat aagacttttt gtgctatatc attatcatta
     3001 gtaccactcc aaaataattt aatatacagc ttactgaaaa tatttccctg tgtcaaaaca
     3061 tcatcatata tattcttaga tgattcatac gcattttgta ttttatccga catatcctac
     3121 ttcgcctttt ttccattctt caatcgcatc cctaatacga acatcaatat ctatgcgtgt
     3181 ttcaaggcat tcctttggat gttttcttgc ggattgaaac gcaaatttca caaagcttgc
     3241 tgaaccaatt ggaagcatca tctttaacat gctttccgga aaaacaaaat gagtcccata
     3301 tttatacaca acagcttcta acttgcatat atgttctcgt tcttccatgc gacgaatgta
     3361 ttttgcagca tcccacaaca catcatcttc agcaccaatc aacaatagtt ttccatgaat
     3421 cttttcaatc ttgatttttt cttcttccaa aatcggatga acggcttctg aatcatcaaa
     3481 caactttcta gaattgatca tatcacccgt tcgctttgtt tcttgttcaa tcacatgcca
     3541 atattgagga tgtttataac aaaatggcat atatagaagt ggtttttctc tatatgaaaa
     3601 tagtgattcc ccttcgattg gccattcttt acaaccatct tttttgcctt gcataaagcc
     3661 ttgccaaata aaatcacttg gagttaagcc aattgtcaaa gtgatatctt caaaataaga
     3721 agctgccgta agtgctagtg ttcctgtagt ggatgcaccc acaattccaa ccttcttatt
     3781 tccatgtact tttagccatc ttatcgcatt ctctattcgt tctaaaggat aattatgatg
     3841 tccataatct ttttttgcgg gtgacatagt cattacattt aatcctagtt tatgtaacca
     3901 ttttacagat gtacgggcca aataatcctg ggaatcatca ccaatcatgg caatcatcgc
     3961 acaatctgaa tctgttttgc atttccaata gtcaccataa aatccatctg tgtgtacttc
     4021 aaaatgaatt ttcttcatcc gtattaactc ctattcttca aaactataat tttatttgaa
     4081 atatttcgaa ccaatggaat ccatccaaac aaatgataaa taggatgcaa ttctttcata
     4141 ccttcaatca aactaacttc cttatcaaaa ataaattctg gtaaaatctt ttgtagttca
     4201 tttctattct ttacacccca taaaaattta gcattacttt gatctatcga cttttctttc
     4261 atatgcttta ctacaaaagg cgacatagtt tcgacaagaa cagttacttt tttaaaggat
     4321 ttttcaataa tagaaaacat cttcaacact tctttttcgt ctaaatacat agttaaccct
     4381 tcaatgatca ctagaatatc ttctccatga tactcaatat catggatata agaatcatcc
     4441 atagctgatt ttgcgatttg atacattggc cttgtatcag taagaaactg ttcccttatc
     4501 ttcatagttt gaggcaagtc aagattatac caacgaagat atttaccttt cattctgtaa
     4561 caacgagtat ccaatccaca tgcaatattt actacaactg catttgtatg aacatttaaa
     4621 tattgttcga ccatacgatc caatacaatc gttcttgcga taacaccact actcatagca
     4681 ctatctttat cagctttaga aaaatcataa tctatatttt caacaatatg tacggcaata
     4741 tcatccttaa tttttgcatt ttgactattc gtttcttttg ctctagcata taaagtctga
     4801 agcattgtct ctgaaacacc tgtaatatct atttttgatt tcataatcca acatctccaa
     4861 ttacaacatt tcttacttct tctatccact tgttagcgtg gcaacaaaac aattcctcat
     4921 gctgcatatt aaatctatga atatccggat tcttaaaata tgtatagtat ctcttttcat
     4981 attttttacc cattttggtc gcatagaata catgaatttt tgtatttgga acatccatat
     5041 tgtgttgtac ttttgtcact aaatccgaat agaattgatt ataaatacta cacttactaa
     5101 tccaaggact tcctaaacca aacatatcta aaaatccatt caccaatttc ttcttcgaat
     5161 catccatttg tgcgatcttt ttctccatga actttggaag tgtttctgta tgcaccattt
     5221 tatacataat aggcgttact atagaggctt gaatggtagc catcaccttt ccagcctcat
     5281 ccatatcact acttccaata atgccatgat ccatatgaat tctttttctt tgaatcaaca
     5341 aagaaacaaa actgcctcca agtgaacatc cataagcggc tttaattctt ccgccataat
     5401 gatccattat ttcttgttca atcttttcac attcttcttc catcgaataa aattgagtat
     5461 tctcatttgg atcaaagcca tcataagaaa cagccaccgt ataaaaatat acatgtaatc
     5521 cttctaacac atgatcaaaa ctactatata ggcaacaagt gcctggaaac aaaaagatga
     5581 ctggtttact ttgatttccg ctttcataaa atttcataat caaccctcaa aaatgttaga
     5641 taaaactaac tttttcttgg tttcagttta ccactgcaat taatgacacg caagaacaat
     5701 atgtagtaca aaagaaaaga caactcctat taagaattgt ccaatatatg atttatttct
     5761 tcaataaatt ataatatcat ttgttgatat agaaaaagag tagaaattgt atcacacaat
     5821 acaatttcta ctctagtttt gtagttatct ttccatttta aattagcgta agtttggcgt
     5881 aaatgcgtaa atttacacca aaaatttctc atttttttat cctagtaaag cttagctcct
     5941 gcaggaatct tgtcatcaac catcaataag ttcaagcatt catgaccatc ttcttcatgt
     6001 acagctgag
//
LOCUS       DBMDWL010000126         5972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5205_length_5972_cov_2.428717, whole genome shotgun sequence.
ACCESSION   DBMDWL010000126 DBMDWL010000000
VERSION     DBMDWL010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5205_length_5972_cov_2.428717"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="ACZPDN_08605"
     CDS             <1..1458
                     /locus_tag="ACZPDN_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1298726.1"
                     /translation="VNKIYTTQFQEQALSDLENEKGNGNYTLQNIFMKYNPFSTNTQS
                     MYVYFNTSKAAKITYTVSCADYADFTATPYQAKEFQKEHEFQLIGLIPDCTNTITITA
                     TYKNGTSQSVSTNYTMGSLLGDEDIQLEQVSGSKQDLGNGLYTLLGNDSDDQDFVYMY
                     DTNGILRSEIPIIGYRSHRMLMQNQTLYMSVSTHRMAAINHLGMIEHIYNLDNYIVHH
                     DYQFDQDGNLILLATNEEKNSVEDCIIKLDVETEEVSELLDLEDLFKSYKESTNHKEG
                     SKWDWMHINTLQYLSDDSVILSSRETSSIIKIKDVSTNPSIDYIIGNEDFWKDTDYTE
                     YLYTKVGDFLTSGGQHTVTYMEDDSLESGQYYLYMFDNNFGYSKTRKDYDWIVNEGIQ
                     TSITEGTTSYYYQYLVDENEGTYTLVNSFEVPFSAYVSSAQNLGEHIVVDSGIAGLLS
                     IYDTDGNLEQQLSIKKNDRFIYRIYYYDYKGYYFS"
     gene            1400..2038
                     /locus_tag="ACZPDN_08610"
     CDS             1400..2038
                     /locus_tag="ACZPDN_08610"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:NF016488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1298727.1"
                     /translation="MIDLFIESIIMIIKDIILVREQSLTFFKAILSVVSYVYGMTYKE
                     FYGSDYDQFKKVLDSVLNTIESSSLACIQYTTSRIKSPESVLKKIGNDYALLHDIIGV
                     RIICSFVDEIYEVKDWINSSFEVVEVRDYLRHPKPSGYRSLHVIIKVDGCFVEIQVRT
                     IALDFWANLEHQIHYKKDVEDEKLIRSELKRCADEIASLDLSFQTIKDRIEG"
     gene            2040..2708
                     /locus_tag="ACZPDN_08615"
     CDS             2040..2708
                     /locus_tag="ACZPDN_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1298728.1"
                     /translation="MRLLIADDEENLAYALKAVLEDAGYNCDVCFDGKSAIRLISQEI
                     YDGLILDIMMPEKNGYEVLSIIRNKKIDIPILLLSALNEVNDKVQGFDLGANDYLSKP
                     FSTKELIARIKVMLKEIIVDDSYLQYQDILLNKKEETLSCKNIKYQLSKYECSILSLL
                     IRSELNRVPVWLVIEKVWQNKVDVDVKTVSLYISYVNRKLKASASKAYIKFDEENIEL
                     CVNI"
     gene            2732..3409
                     /locus_tag="ACZPDN_08620"
     CDS             2732..3409
                     /locus_tag="ACZPDN_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1298729.1"
                     /translation="MNILVVDDEKNIAYAIAQILEEQKWQVTMAYDGQEGLDLALSGY
                     YDVAILDIMLPGMNGFEIVQKMRANKVETPTLLLSALDEIPDKVKGLNVGADDYMTKP
                     FSAAELVARINALTRRKGEVIVHELKYEDLSLQLDTCELYCASHHIHLGYKEFEIMKQ
                     LMSHPEMITSKDDLIISVWGSESDAMDNNVEVYISFLRKKLKFLKSQVSIKVIRKVGY
                     RLEVCDA"
     gene            3402..4634
                     /locus_tag="ACZPDN_08625"
     CDS             3402..4634
                     /locus_tag="ACZPDN_08625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1298730.1"
                     /translation="MLKKLQTKFILILMSFVSVILLSVFGIVCCSNYVHDKKDVERSL
                     EMALRLKDKDFSISFGNSRNFNGNSFVIYTDSNYEYMITSRTVWNIYAEDAQSLIESV
                     EHKEDVGVLKKLGFAYKKEKITNYQVNEDTLIPSGYAVAFVDISNMQSSFQRMLIISV
                     QIGSSVLLVFFILSCILSKWALKPVEQAWDKQKQFVADASHELKTPLTVILADSDVLL
                     NHSTESISSQKKWIESIQSEANRMRKLVENMLFLAKNDANRIEISMQNCSLSDICFHC
                     VLPFESIAFEKGIDLIDEVEENIEVHGNESQLKQLILIFLDNAIKYTPSGGKIYFKLA
                     KIQNKPVLTIRNTDSYIPPEDLKHVFERFYRVDKSRKYQGGAGLGLSIASQIAQSHKI
                     KLSVTSDEKQGTEFTLNF"
     gene            4645..5718
                     /locus_tag="ACZPDN_08630"
     CDS             4645..5718
                     /locus_tag="ACZPDN_08630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1298731.1"
                     /translation="MFQKRVDQVIEKMKADGLKQILVSEPCSIYYLTGVDVGPGERLF
                     ALYLNDEGRKVLFLNTLFTVEQKDCEEIWYSDTDDSISMLASVIDSKETLGIDKEWTA
                     RFLIPLMEKCKGLKVVLGSKYVDATRAIKDEKEIECMIENSQINDVVMERTRDFISEG
                     MTEKQVEAFIVEQYKLLGCQDVSFSPICSFGANGADPHHMPDDSVLKAGDSIVIDIGG
                     RKDRYCSDMTRTYFCKEASEEYKKIHDIVRVANEKAEKIIRPGVRLCDIDLTARNYIS
                     SFGYGEYFTHRLGHFIGQTDHEFGDVSSTNTETVKPGMIFSIEPGIYLPNKMGVRVED
                     LVLVTEDGCIVLNKVDKKYAMIG"
     gene            complement(5736..>5972)
                     /locus_tag="ACZPDN_08635"
     CDS             complement(5736..>5972)
                     /locus_tag="ACZPDN_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1298732.1"
                     /translation="QNDLLKEDPLLSNERQIGLLNLAKEDMLRAKEAMNRMVEPDLIE
                     MDIQAAHDHLKEILGEVHREDLLDTLFSKFCLGK"
BASE COUNT         2114 a          759 c         1166 g         1933 t
ORIGIN      
        1 gtcaataaaa tctatacgac tcagtttcaa gagcaggcat tatcagattt agaaaatgaa
       61 aaaggaaatg gaaactatac acttcaaaat atatttatga aatacaatcc tttttcaacc
      121 aatacacaat cgatgtatgt ttattttaat acatccaaag cggcaaaaat tacatataca
      181 gtaagttgtg ctgattatgc ggattttaca gctacacctt atcaggcaaa agaatttcaa
      241 aaagaacatg agtttcaatt gattggattg attcctgatt gtacaaatac aatcacaatt
      301 acagctactt ataagaatgg tacaagtcaa agtgttagta caaattatac aatgggatca
      361 cttttaggtg atgaagatat acaacttgaa caagtgtctg gttctaaaca agacttaggc
      421 aatggtttat atacactatt aggaaatgat tccgatgatc aagactttgt ctatatgtat
      481 gatacaaatg gcattttgcg ttcagaaata cccattattg gatatcgttc tcatcgtatg
      541 ttgatgcaaa atcaaacatt atatatgagt gtatcaacac atcgaatggc agctattaat
      601 catttgggaa tgatagaaca catttataat ttggacaatt atatagttca tcacgattat
      661 caatttgatc aggatggaaa tttgatttta ttagctacaa atgaagaaaa gaatagtgta
      721 gaagactgca ttattaaatt agatgtagaa actgaagaag taagtgaatt attagattta
      781 gaagatttat ttaaaagcta caaagaaagc acgaatcata aggaaggctc aaaatgggat
      841 tggatgcata tcaatacact tcaatatcta agtgatgata gtgttattct ttcttctcga
      901 gaaacaagtt caatcattaa aatcaaggat gtaagcacaa atccaagtat tgattatatt
      961 attggaaatg aagatttctg gaaagatacg gattatacag aatatctata tacaaaagta
     1021 ggtgatttct tgacaagtgg aggacaacat actgtaacct atatggaaga tgattcgctt
     1081 gaatcaggtc aatattatct atacatgttt gacaataatt ttggttattc aaagacaaga
     1141 aaagattatg attggattgt aaatgaagga attcaaacaa gtattacaga aggtacaaca
     1201 tcgtattact atcaatattt agtggatgaa aatgaaggta catatacatt ggtcaattca
     1261 tttgaagttc cgtttagtgc ctatgtatcc agtgcacaaa atctagggga gcatattgtc
     1321 gtggattcag gaattgcagg actattatcc atttatgata cggatggaaa tcttgaacag
     1381 caattgagta taaagaaaaa tgatcgattt atttatcgaa tctattatta tgattataaa
     1441 ggatattatt ttagttagag agcaatctct aacttttttt aaggcaattt taagtgttgt
     1501 ttcgtatgtt tatggtatga catataaaga attttatgga tctgactatg atcagtttaa
     1561 gaaagtactc gattctgttt taaatacgat tgaatcgagt tcattagctt gtatacaata
     1621 tactacttct aggatcaaat cgccggaaag tgttttaaaa aagattggca atgactatgc
     1681 attacttcat gacattattg gtgttcgcat catttgttca tttgtggatg aaatctatga
     1741 agtaaaagat tggatcaatt ctagttttga agtggtggaa gtaagagact atttaagaca
     1801 tcccaaacca agtggatatc gaagtctaca tgtgattatt aaagtagatg gatgtttcgt
     1861 tgaaattcag gtgcgaacga ttgcacttga tttttgggca aatctagaac atcaaattca
     1921 ttataagaaa gatgttgagg atgaaaaatt gattcgtagt gagttaaaac gatgtgccga
     1981 tgaaattgct tctttagact tatcatttca aacaattaaa gatagaatcg agggataata
     2041 tgcgattgtt gattgcggat gatgaagaaa atttggctta tgcattaaaa gcggttttag
     2101 aagatgcggg ctataattgc gatgtgtgtt ttgatggaaa gagtgccatt cgtttgattt
     2161 ctcaagaaat ttatgatgga ttgatcttag atattatgat gccagaaaag aatgggtatg
     2221 aagtattgtc tattatacga aataaaaaga tagatattcc tatattactt ttaagtgcat
     2281 taaatgaagt gaatgataag gttcaaggtt ttgatttagg agccaatgat tatttatcca
     2341 agcccttttc gacaaaggaa ttaattgctc gaatcaaagt gatgttaaaa gaaattatag
     2401 tggatgattc ttatttacaa tatcaagata ttctattgaa taagaaagaa gagacgcttt
     2461 cttgtaagaa tataaaatat caattgtcta aatatgaatg tagtattctt tctttgttga
     2521 ttcgaagtga attaaatcga gtacctgtat ggcttgtgat tgaaaaagta tggcaaaata
     2581 aggtggatgt agatgtaaaa actgtttcac tttatatatc ttatgtaaat agaaaattaa
     2641 aagcctcagc ttcgaaagca tatataaaat ttgatgaaga aaacatagaa ctgtgtgtta
     2701 atatataaaa aagaagaaag ggcgggaaaa tatgaatata ttagttgtgg atgatgaaaa
     2761 aaatattgcg tatgcgattg cacaaatatt agaagaacaa aaatggcaag ttacgatggc
     2821 ctatgatggt caagaaggtt tagaccttgc gctaagcgga tattatgatg tagctatttt
     2881 agatattatg cttccgggta tgaatggttt tgaaattgta caaaagatgc gtgctaataa
     2941 ggttgaaact cctacactat tattatcggc attagatgaa attccagata aagtcaaagg
     3001 cttaaatgta ggtgcagacg actatatgac aaagccattt tcagctgctg aacttgtagc
     3061 ccgcatcaat gcactgacaa gacgaaaagg ggaagtcatc gttcatgaat taaaatatga
     3121 agatctttct ttacaacttg atacttgcga gttgtattgt gcgagtcatc atattcattt
     3181 gggatataaa gagtttgaaa taatgaaaca attaatgtcg catccagaaa tgattacctc
     3241 taaagacgat ttgattatct ctgtatgggg aagtgaaagt gatgcgatgg ataataatgt
     3301 cgaagtatat atttcattct taagaaagaa attgaagttt ttaaagagtc aagtttcaat
     3361 caaagtgatt cgaaaagttg gatatcgact tgaggtatgc gatgcttaaa aaattacaga
     3421 caaaattcat tttgatttta atgtcctttg tcagtgtaat tctactatct gtatttggca
     3481 tagtttgttg ctcaaattat gtccacgata aaaaggatgt cgaacgaagt ttggaaatgg
     3541 ctttgcggtt aaaagataaa gatttttcga tttcatttgg aaatagtcgt aattttaatg
     3601 ggaattcctt tgtgatctat acagattcta attatgaata tatgatcaca agtcgtacag
     3661 tatggaatat atatgcagaa gatgcacaaa gtttgattga aagtgtggaa cataaagagg
     3721 atgtgggcgt actaaaaaaa cttgggtttg cgtataaaaa agaaaagatt acgaactatc
     3781 aagtgaatga ggataccctt attccttctg gttatgccgt agcctttgtg gatatatcga
     3841 atatgcagtc ctcttttcaa agaatgttga ttatatctgt acaaattgga tcgagtgtat
     3901 tacttgtatt ctttatatta agttgtattt taagtaaatg ggcactaaaa ccagttgagc
     3961 aagcatggga taaacaaaaa cagtttgtcg ccgatgccag ccatgaattg aaaacgccat
     4021 taaccgttat tttagctgat agtgatgtgt tattaaatca ttctacagaa tctatttctt
     4081 cacagaaaaa atggatagaa tccattcaaa gtgaagcaaa tcgaatgcgt aagcttgtag
     4141 aaaatatgtt gtttcttgcc aaaaatgatg cgaatcgaat tgaaatttcg atgcagaatt
     4201 gttctttgag tgatatatgt tttcattgtg ttttaccatt tgaatcgatt gcgtttgaaa
     4261 aaggtattga tttaatcgat gaagtggaag agaatataga ggtacatgga aatgagtcac
     4321 aattaaaaca attgattttg attttcttgg acaatgcgat caaatataca ccttcaggtg
     4381 gaaagatata ttttaagttg gcaaagatac aaaataaacc cgttcttacg attcgtaata
     4441 cggatagtta tattccgcct gaagatttaa aacatgtatt cgaacgtttc tatcgtgttg
     4501 ataaatcaag aaaatatcaa ggtggagccg gtttaggtct atcgatcgca agtcagattg
     4561 cacaaagtca taaaattaaa ttaagtgtga ctagtgatga aaaacaaggt acagaattta
     4621 ctttgaattt ctaggaggaa aattatgttt caaaaaagag tcgatcaagt aattgaaaaa
     4681 atgaaggcag atgggcttaa acaaatctta gtgagtgaac catgcagtat ctattattta
     4741 accggtgtgg atgtaggtcc aggagaaaga ttgtttgcgc tatatttgaa tgatgaagga
     4801 agaaaagtat tatttttaaa tacattattt acagttgaac aaaaggattg cgaagaaatt
     4861 tggtacagtg atacagacga ttcgatttca atgttggcaa gtgtgatcga ttctaaagaa
     4921 acacttggca ttgataaaga atggacagca cgcttcttga ttcctttaat ggaaaagtgt
     4981 aaaggattaa aagttgtgtt aggatctaaa tatgtggatg ctactcgtgc tattaaagat
     5041 gaaaaagaaa ttgaatgcat gattgaaaat tcacagatca acgatgtggt gatggaacgt
     5101 acaagagatt tcatttcaga aggtatgact gaaaaacaag tggaagcttt tattgttgaa
     5161 caatataaat tgttaggatg tcaagacgtg tcattctcac ctatttgttc atttggagca
     5221 aatggtgccg atcctcacca catgccagat gattctgttt taaaagccgg agatagtatt
     5281 gtgatcgata ttggtggaag aaaagatcgt tattgttcgg atatgacacg tacttatttc
     5341 tgtaaagaag caagtgaaga atataaaaag attcatgata ttgttcgtgt tgccaatgaa
     5401 aaagcggaaa aaatcattcg tccaggcgtt cgtctatgtg atattgattt aacagcacgt
     5461 aattatattt caagttttgg ctatggcgaa tactttacac accgccttgg acattttatt
     5521 ggtcaaaccg atcatgaatt tggagatgta agttctacaa atacggaaac tgtaaaacca
     5581 ggcatgattt tctcaattga accaggcatt tatttaccta ataaaatggg tgttcgtgta
     5641 gaagacttag ttttagttac tgaagatggc tgtattgtat taaataaagt agataaaaaa
     5701 tatgcgatga ttggctaatc atcgcatttt tcttactatt tacctaaaca gaatttagag
     5761 aataaagtgt ccaataaatc ttctctatga acttcgccta agatttcttt caagtgatca
     5821 tgagctgcct gaatatccat ctcaattaaa tcaggctcta ccatcctgtt catggcttct
     5881 ttcgccctta acatatcttc tttggctaga ttcaataaac caatttgacg ctcatttgaa
     5941 agtaatggat cttcttttaa taaatcattt tg
//
LOCUS       DBMDWL010000127         5967 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5213_length_5967_cov_2.132117, whole genome shotgun sequence.
ACCESSION   DBMDWL010000127 DBMDWL010000000
VERSION     DBMDWL010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5967)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_5967_cov_2.132117"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="ACZPDN_08640"
     CDS             <1..213
                     /locus_tag="ACZPDN_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298733.1"
                     /translation="YKRVHINKVFGFDPSDNFNFKYAYGVCSLKKGWFYYLDRIHTSR
                     DIIFNEENIELLSDGVCKLVKTTRTF"
     gene            403..981
                     /locus_tag="ACZPDN_08645"
     CDS             403..981
                     /locus_tag="ACZPDN_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RQC-minor-1 family DNA-binding protein"
                     /protein_id="HIR1298734.1"
                     /translation="MSKKVRRVPVILDAGEIKDLPQEDIQMILRGADELISTGGRSML
                     AKILKGSKDKKIFEHKLNECPAYGYYRDMKLDDIAKCIDWMIKKDYLRIEYDYRLPLL
                     VFSEKGWQIEKETFAQELYQRICLDVEEKKARVLFEMKEVNRQVVMRVLDKIEKDGTE
                     KFLPYLEAWKMLEVKKVAARIAEVENKISRRI"
     gene            991..1527
                     /locus_tag="ACZPDN_08650"
     CDS             991..1527
                     /locus_tag="ACZPDN_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013375.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1298735.1"
                     /translation="MKILIFGMGNIGKSTVGELLAKKIGYDFIDMDTKIKEKYGTMLG
                     FQDEYNDQYERDELRAEMISSWIQENENVVIALSPIAYLDAYEDFFEDSDIICFDLTD
                     RTENIFKRLVFTDDNDNLLHIPQSYLNKHKAYYMREIQADFDYFHTLYASKMDSISMD
                     DKSLDEIVEKICKKYKLV"
     regulatory      1559..1645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1689..2450
                     /locus_tag="ACZPDN_08655"
     CDS             1689..2450
                     /locus_tag="ACZPDN_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1298736.1"
                     /translation="MKKNRRIVMPLLVFILVIGGWELYVRTFDISLYILPSPSKIISA
                     LIENMDILMQHSLVTLEESVLGLLISTFFAIVISIGMDLSKTFKTSIYPYLIVTQTVP
                     IMVLGPLFSIWFGFDLLPKILIVIFMCFFPIAISFTDALSQVSEKEINLLKSFGANLF
                     QIYKIVKIPSGAIGLFSGLKVAATYCVGGAIVGEWLSSRAGLGYYMLRVKNGYMLDKV
                     FACVLMIIFWSILLNLGVSLLENILFPHLRKGERK"
     gene            2447..3445
                     /locus_tag="ACZPDN_08660"
     CDS             2447..3445
                     /locus_tag="ACZPDN_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1298737.1"
                     /translation="MKILKAMCAAAVALSLVGCAQTSNSDDKLTKVTLMLDYTPNTNH
                     TGFYVAKNKGYYKEAGLDVDIIEPGDNATTSMVAAGKADFGVSYQEDVTYALTSEDPL
                     PIKTIATIIQHNTSGFVSLKSANINSVKDFEGKTYAGWGAPSEEAVIKAVMNEAGADF
                     SKLNIVGSDGSGIAGLSDEVGKNKVNLGWEFYGWAIIKAMQDGYDLNYMPLADLDSRL
                     DYYTPVIITNNKMIKKNSDTIQKFMEATKKGYEYAIDHPSKAAEILHDDALTDTDLKF
                     LKESQKYLSSQYAKDADSWGVMKDSVWDNYTDFMYETGLIDHKIDAKDQYTNEFVK"
     gene            3445..4179
                     /locus_tag="ACZPDN_08665"
     CDS             3445..4179
                     /locus_tag="ACZPDN_08665"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298738.1"
                     /translation="MKLQVEKLNLEFSGKEILRDISFSIQEGEFVSILGPSGCGKSTI
                     LNILAGLIEDYSGQLFVDDERIKGVSSHFAYMPQNDLLLEWRTILDNVCLYGEINHDK
                     SMKQRALKEFETFGLSGYENAYPSSLSGGMRQRAAFLRTSLCKADILLLDEPFGALDV
                     ITRNDMQDWLLQLRKNYNRTTLLVTHDIDEALYLSDRILILSNKPSRILQEIDLSKEK
                     KSRDWLFSQSVLKKQVYELLKGENHA"
     gene            4172..4855
                     /locus_tag="ACZPDN_08670"
     CDS             4172..4855
                     /locus_tag="ACZPDN_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1298739.1"
                     /translation="MHDAHYHYSKEINALQNEFDISGICNVANEKEFELVQQKRLLYS
                     CGVHPWNASLDTLNSMFPLLKNAPIIGEIGMDSVWCDVDLKIQKEVFEKQLQLAQALN
                     KPVILHTKGQEKTILELIRKYPNTYVVHWYGCMDYVKEYDEVVSYFTIGPSIGKEEEV
                     THLVKQISMDKLLMESDGIEAVKWAIDSDDYLAALKNEITMVALLKNQSYSEVESILD
                     HNFSKLIKK"
     gene            complement(4870..>5967)
                     /locus_tag="ACZPDN_08675"
     CDS             complement(4870..>5967)
                     /locus_tag="ACZPDN_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014542.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1298740.1"
                     /translation="TGYLGAGKTTLLNHILKNQDGYKVAIIVNDIGEINVDQKLIDKN
                     AQIQKEDTDVVGLTNGCICCTLKLDLLQQLSALASSNKFDYIIIEASGICEPIPIAQT
                     ITAANETLYSRGLPELFHLDNIICVADAARLSNEFNCGKHLLEPVEDEDSLDSLLMQQ
                     LEFCNTVILNKAETVNEDEKNEIKAVIHKLCKDAGIIEASFGQVNLEELLNTHNFDYE
                     KAQNNIGWLHEIEEHEHHHDHEHEGEALEYGISTFVWQTRKPLDLKKFTNLLNNYPNV
                     IRTKGYVWFDNQPDSAYLFEQAGKLTSLQEDSLWIASAPKQDQIDLLKANPQLAKNWD
                     EEYGDRINQLVFIGKNMDEEQIKRQFNDCLA"
BASE COUNT         1989 a          771 c         1205 g         2002 t
ORIGIN      
        1 tataaaagag tacacataaa taaagtattt ggatttgatc catccgacaa cttcaacttt
       61 aaatatgcat atggagtatg cagtttaaag aaaggttggt tctattatct tgatcgaata
      121 catacttcta gagatataat tttcaatgaa gaaaatattg aacttctaag tgatggtgta
      181 tgcaagcttg ttaaaacaac ccgtacattc tgatgaatat tctcgtttat agatgttttc
      241 agaaaaagaa atatttgtcc aagaacttta tcaaagaatg tgcttagatg aagaagaaag
      301 aaggtcgtgt catttttgaa atgaaagaag taaattggtt ctatgtagga catttagtgt
      361 tatgtggatt gattcgtgta atgatgtaga ggtaaagaat ggatgagtaa aaaagtacgt
      421 agagtgccag ttattctgga tgcaggagaa attaaagatt taccgcagga agatatacaa
      481 atgattttaa gaggggcaga tgaattgatt tcaactggcg gaagaagtat gttggctaaa
      541 atattaaagg gttcaaagga taaaaagata tttgaacata aactaaatga atgtcctgct
      601 tatggatact atcgggatat gaagttggat gatatcgcaa aatgcattga ttggatgatt
      661 aagaaagatt atctcagaat tgaatatgat tatcgtcttc ctttattggt attttcagaa
      721 aaaggatggc aaatcgaaaa agaaacattt gcccaagaac tttatcagag aatctgttta
      781 gatgtagaag aaaaaaaggc tcgtgttcta tttgagatga aagaagtaaa tagacaggtt
      841 gtcatgcgtg tgcttgataa aatagagaaa gatggaacag agaaatttct accttactta
      901 gaagcatgga agatgttgga agtgaaaaaa gttgcggcaa gaattgcaga agtagaaaat
      961 aagataagta ggcgtatata gaggtgcaaa atgaaaatat taatatttgg gatgggtaac
     1021 attggaaaga gcactgttgg tgagttgttg gcaaagaaaa taggttacga ttttatcgat
     1081 atggatacga aaatcaaaga aaagtatgga acaatgcttg gttttcaaga tgaatataat
     1141 gatcaatatg aacgggatga attgcgtgca gaaatgattt cttcttggat tcaagaaaat
     1201 gaaaatgtag tgattgcctt aagtcctatt gcgtatttag atgcatatga agatttcttt
     1261 gaagattcag atattatttg tttcgactta acggatcgaa cagaaaatat ttttaaacgc
     1321 ttggtattca cggatgataa tgataatctt ttgcacatcc cacaatcgta tttaaataag
     1381 cataaggcat attatatgcg agaaatacag gcggattttg attatttcca tactttgtat
     1441 gcatcaaaaa tggattcgat ttcaatggat gataaaagtt tagatgaaat tgtagagaag
     1501 atatgcaaga aatataaatt agtctaagat tgtggtataa tctttaggca agtaaaaaca
     1561 tacatgacag gggtgcaatt gctgagaaaa acccttatta cctgatctgg ttaattctag
     1621 cgtagggagt catatcgatt gatagtggca ctctagggtg ccttttttat tttggaaagg
     1681 aggcaatcat gaagaaaaat cgtagaattg tcatgccgtt gcttgtgttt attctagtga
     1741 ttggtggatg ggaattatat gttcgtacat ttgatatttc actttatatt ttgcctagtc
     1801 catcaaaaat tatcagtgca cttattgaga atatggatat tttgatgcaa cattcgcttg
     1861 ttacattgga agaatctgtt ttaggtttat tgattagtac tttctttgcg attgtgattt
     1921 ccattggcat ggatttatcc aaaacattta agacaagtat ttatccatat ttgattgtga
     1981 cacaaaccgt tcctattatg gttttaggac ctttgttttc aatttggttt ggatttgatt
     2041 tgttgcctaa aatcttgatt gttatattta tgtgcttttt tcctattgcg atatccttta
     2101 cggatgcttt gtcgcaagtg agtgaaaaag aaatcaattt attaaaaagt tttggtgcca
     2161 atctatttca aatctataaa attgttaaga ttccttctgg tgccattggc ttattttcag
     2221 gattaaaagt agcggctact tattgtgttg gcggagctat tgtaggcgaa tggctttcaa
     2281 gtagagcagg acttggctat tatatgcttc gtgtgaaaaa tgggtatatg ttggataaag
     2341 tttttgcgtg tgtattgatg attatatttt ggagcatcct attgaattta ggagtgtctt
     2401 tattggaaaa tatattgttt ccacatttac gaaaaggaga aagaaaatga aaattttaaa
     2461 agcgatgtgt gcagctgctg ttgcgttaag cttagttggt tgtgctcaaa catcaaattc
     2521 agatgacaaa ttgactaagg ttacattgat gttggattat acgccaaaca caaaccacac
     2581 tggtttttat gtggcaaaaa acaaggggta ttataaagaa gctggtttag atgtagatat
     2641 tattgaacca ggtgataatg cgacaacttc tatggttgca gccggaaaag ctgattttgg
     2701 tgtttcttac caggaagatg ttacatatgc cttgacaagt gaagatccac ttccaattaa
     2761 aacgattgcg acaattattc aacataatac ttctggattt gtgtctttga agagcgcaaa
     2821 catcaattct gtaaaggatt tcgaaggaaa gacatatgca ggatggggag caccaagtga
     2881 agaagccgtt attaaagcgg ttatgaatga agcgggtgct gattttagta aattaaatat
     2941 tgtaggatcc gatggatctg gtattgcagg acttagtgat gaagtaggaa agaataaagt
     3001 gaatcttgga tgggaattct atggttgggc tattattaaa gctatgcagg atggatatga
     3061 tttaaactat atgcctctag ctgatttaga tagccgtttg gattactata ctccagtaat
     3121 tatcacaaac aataaaatga ttaaaaagaa ttcggataca attcaaaaat ttatggaagc
     3181 tacaaaaaaa ggatatgaat atgcgattga tcatccaagt aaagcagctg aaattttaca
     3241 tgatgatgct ctgacagata cagatttgaa attccttaag gaaagccaaa aatacttaag
     3301 tagccaatat gcaaaagatg cagattcatg gggtgttatg aaagattctg tatgggataa
     3361 ttatacagat tttatgtatg aaacaggatt gattgatcac aagatcgatg cgaaagacca
     3421 atataccaat gagtttgtaa aataatgaag ctacaggttg aaaaactaaa tcttgaattt
     3481 tctggaaaag aaatattgcg cgatatttct ttttctattc aagaaggaga atttgtttcc
     3541 attctaggtc cgagtggttg tggaaaatct actattttaa atatattagc cggattaatt
     3601 gaagattatt caggacagct ttttgtggat gatgaacgta ttaaaggtgt cagctctcat
     3661 tttgcgtata tgccacaaaa tgatttgttg ctcgaatgga gaaccatttt agataatgta
     3721 tgtttatatg gtgaaatcaa tcatgataaa tctatgaaac aaagagcttt aaaagaattt
     3781 gaaacatttg gcctttctgg atatgaaaat gcatatcctt ctagtctttc aggaggaatg
     3841 cgccaaaggg cagccttctt aaggacttct ttatgtaaag cggatattct tttattagat
     3901 gaaccatttg gagctttgga tgtcatcacg cgtaatgata tgcaagattg gttgttacaa
     3961 ttgagaaaga attataatcg tacgacttta ttagttacac atgatattga tgaagcttta
     4021 tatttatcgg atcgtatttt gattttatcg aataagccaa gtcgtatttt gcaggaaatt
     4081 gatttatcga aagaaaagaa atcaagagat tggttatttt ctcagagtgt tttgaaaaaa
     4141 caggtctatg aattattaaa aggtgaaaat catgcatgat gcacattatc attattcaaa
     4201 agaaataaat gcgctacaga atgaatttga tatttctggt atttgtaacg tcgcaaatga
     4261 aaaagaattt gaacttgtcc aacaaaagcg gttgctctat tcttgtggag tacatccttg
     4321 gaatgcaagt ttagatacct tgaattcgat gtttcctttg ttaaaaaatg cacccattat
     4381 aggggaaatt ggcatggata gtgtatggtg tgatgtagat ttaaaaattc aaaaagaagt
     4441 atttgaaaaa caacttcagc ttgcacaagc attaaataaa cccgtcattc ttcatacaaa
     4501 gggtcaggaa aaaacgatac ttgaattgat tcgtaaatac ccaaatacat atgttgtaca
     4561 ttggtatgga tgtatggatt atgttaaaga atatgatgag gtggtatctt attttacgat
     4621 tggtccttcc attggaaaag aagaggaagt aacgcattta gtaaaacaaa tatctatgga
     4681 taaattactt atggaatccg atggtattga agctgtgaaa tgggctattg atagtgacga
     4741 ttatctggct gctttaaaaa atgaaattac gatggtggct ttgttaaaga atcaatcgta
     4801 ttctgaggtt gaaagtattt tggatcataa cttttcaaaa ttaataaaaa aataggagaa
     4861 tttcttctcc tatgcaagac agtcattgaa ctgtcttttt atttgttctt catccatatt
     4921 ctttccaata aagaccaatt gattgattcg atctccatac tcttcatccc agtttttggc
     4981 tagttgagga ttggccttta ataaatctat ttgatcttgt tttggtgctg atgcgatcca
     5041 taatgagtct tcttgaaggg atgttaactt accggcttgt tcaaataagt aagcagagtc
     5101 aggttgatta tcaaaccata cataaccttt agtacgaatg acgtttggat agttgtttaa
     5161 tagatttgtg aattttttta aatccaatgg ctttcttgtt tgccatacaa aagtagaaat
     5221 accatattct aaggcttcac cttcgtgttc atgatcatgg tggtgttcgt gttcttcaat
     5281 ttcatgaagc cagccaatgt tattttgagc tttttcataa tcaaagttat gtgtgtttaa
     5341 aagttcttct aaattaactt ggccaaagct agcttcaatg attccggcat ctttgcataa
     5401 tttatgaatg actgccttga tttcattttt ctcatcttca ttaacggttt ctgctttgtt
     5461 taaaattaca gtattacaga attcaagttg ctgcatcaat aaagagtcta aagaatcttc
     5521 atcttctact ggttctaata agtgtttacc acaattgaat tcattcgata atcttgccgc
     5581 atcggcaaca caaataatgt tatctaagtg aaataattca ggtaatcctc tagaatatag
     5641 tgtttcattg gcagctgtga tggtttgcgc gatagggata ggttcacaaa taccactggc
     5701 ttcaataata atataatcaa atttgttgga tgaagctaag gcagaaagtt gctgtaaaag
     5761 atctaatttt agtgtgcaac aaatacatcc gttggtaaga cctacaacgt ctgtatcttc
     5821 tttttgaatt tgtgcatttt tatcaatgag tttttggtca acattgattt caccaatatc
     5881 attaacgata atcgcaactt tatatccatc ttgatttttt agaatgtgat ttaataaagt
     5941 agttttacca gctcctaaat agccggt
//
LOCUS       DBMDWL010000128         5912 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5295_length_5912_cov_1.685768, whole genome shotgun sequence.
ACCESSION   DBMDWL010000128 DBMDWL010000000
VERSION     DBMDWL010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5295_length_5912_cov_1.685768"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            539..2959
                     /locus_tag="ACZPDN_08680"
     CDS             539..2959
                     /locus_tag="ACZPDN_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1298741.1"
                     /translation="MKFFKRQKKNNMELDEVYRLLKGYYRCSNKEGYPFLFMSDDFLN
                     TLHWTQKEIETEFDNKLVNMLHPEDRSRLEKNRDSLKNPYKVGSCQNVIFRLKGKDGY
                     HWVSDSYSALKQDSAPFFQGNITDVTSFMLDKEKRAEERNQMLTALTMEYSTVVMGDL
                     MENVVVVVKHDGHMYDHGGLDDFSSEDKMNYTRCLRDFYDRVLVKESCKDFMDILSPV
                     PFMNALLKNETVELQYQIYPNSNGYESLYARAIRLYDEKHHFRFVMGFRPMDEVRKKE
                     NVLELQREIIEGISKDYFSVLLVDLENGQIYSYREAGSNGKRIADFCRSYHDRWTELL
                     PAYAKDLVSDASRENFLEELSFEKLKSTNEDYSFTYEYMSDEGVLYYQARVSYVKKKD
                     RTRAVVIGTRDIDDLIQKERLQKEQLRKAYIQAENASKAKTDFLNSMSHDIRTPMNVI
                     LGYNQLMKKKLSDPVLVDYQKKIEQSGNLLLSIINDVLNMARIESGKSVLVESSESIV
                     DMVEEVSNVFGAMAKEKEIVFDTKMDIKHKQVLCDCTKVKEILVNLIGNAIKYTPNGG
                     HVTVDVKELECSKEGYGTYQIRIIDTGIGMSESFIPTLFDPFTREATGDIIGTGLGMP
                     IVKKLVDMMGGTIDVESKLGKGSTFTITIDHKLGNKEKDKVKQEKLVDSKHSLEGKHV
                     LLAEDNELNAEFAKIILEDCGLHVDLARDGLECVSKVIQNPVGTYDFILMDIQMPNMN
                     GYLATQKIRSIPEKNTIPIIAMSANAFDEDKKKALKAGMDGYISKPIDMEKLQSQIIE
                     ILKEKAGS"
     gene            3131..3802
                     /locus_tag="ACZPDN_08685"
     CDS             3131..3802
                     /locus_tag="ACZPDN_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1298742.1"
                     /translation="MKMITGVSKMCCDGVIQYLEKEKVSILIKDRHTCLDLNDSNMYV
                     LKKGIVKVSLIMQDEREFNITYLQGPDVVSLYCDCLTQFRDCLPKIRVESEQAELYCI
                     SKEKFYKRVKEDANLQNYVNTYYRNRLKIAITREQVMIMNSKAGAVCSWLYRLASLFG
                     IQTQKGIFIDLQVTNEDIANFCGITTRNSVNRVIRGLKEKHIIQVKNNRIIVLDMDYL
                     KQFTK"
     gene            4236..4874
                     /gene="lepB"
                     /locus_tag="ACZPDN_08690"
     CDS             4236..4874
                     /gene="lepB"
                     /locus_tag="ACZPDN_08690"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1298743.1"
                     /translation="MEKRDLRHLKRKELIDLIDKMQTESDLVSNVEVKEELGRLKERE
                     KYLKHLRKTLSVLVVVAAISVLVATLWMPVLKIYGSSMDPTLENGQVVVSIKTKKLKS
                     GDVVAFWQGNKLLVKRVIAGPGQKVDIDVNGKVSVDGKAIHETYLDSESLGNTDIDFP
                     HQVEDSRWFCMGDNRESSIDSRSAAIGDISKEQIEGKVLFSVWPLNKIGIVK"
     gene            4894..5868
                     /locus_tag="ACZPDN_08695"
     CDS             4894..5868
                     /locus_tag="ACZPDN_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298744.1"
                     /translation="MQEYLRRLLKDKARLRKWKRIMIALSCIVVVCTVYALSLPAQTL
                     ACDKEEHTHTAECYDENNELICEKEEHTHNEDCNKQEEVANDEPETQNKDEQVSQVSE
                     EEVEATTTTTTETTTETTKQPFDLSYHTENIESVKFVYKKTEIIDGKEKTTEYDVPTD
                     GSILSYDTLDMTYKLFFKGISAKTLKECGGIIMYQLPNGFKIRKMIEGKTILSGGVVI
                     GNMNVSTDGLVTITYNQQFLSRLQENETLTGSFEVDAQIDMNKIDSSTGKVTITTPKG
                     NITLNYGLYYKEHYGSVSVDKSCKKEATSDYIKYTITLTAGDDGCCYT"
BASE COUNT         2100 a          662 c         1287 g         1863 t
ORIGIN      
        1 tgtgcgtcca tatggtgtat ttgtttctga agtggattat gtaaagcaga agtattgttt
       61 tgaatttgta tatttatata tgataaatta atttatgata aactggttta tactacttgt
      121 ttactagata ttaagagaag taaaaaagct gacaagtatg tgggatttgt cagcttaagt
      181 tggttacaag ccaaggatta gtactcagcc aatttcatta tagcatctaa aaataaaaaa
      241 atggagaact agctatgttt agtttttggg aagggctagt cctcatgtcg aaatgtgtta
      301 gatgtataaa gattgggctt ctttaatttt tatctaacgg ataaattata acatagctta
      361 aaatgtgtgc aatagtaaaa tataactaaa agtattttaa atgcattaaa aaggattaaa
      421 taatatacaa acaaaacatg aaatataacc gtgtaattat ttctttattt tatcgtgaca
      481 aatgaagttt gtgttgttat tatacttgta agggtttaca tgaaattcgg ggtataatat
      541 gaaatttttt aagcgtcaaa agaagaataa tatggagttg gatgaagtat ataggttgct
      601 taaaggatac tataggtgtt ctaataaaga gggatatcct tttttgttta tgagcgatga
      661 ttttttaaat actttacatt ggacgcaaaa agaaattgaa acggaatttg ataataagtt
      721 agtgaatatg ttacatcctg aagatagaag tcgtctagaa aagaatagag attctttaaa
      781 gaatccatat aaggttggtt cttgtcagaa tgtaattttt cgcttgaaag gtaaggatgg
      841 gtatcattgg gtatcggatt cttattcagc tttaaaacag gactctgcgc ctttctttca
      901 ggggaatatt acggacgtta caagttttat gttggataaa gaaaaaaggg cagaggagcg
      961 taaccaaatg cttacggcac tgacaatgga atattcaacg gttgtcatgg gtgatttgat
     1021 ggagaatgtt gtggttgtcg ttaagcatga tgggcatatg tatgatcatg gtgggcttga
     1081 tgatttttca agtgaggata agatgaatta tacacgttgt ttacgtgatt tctatgatcg
     1141 tgtgttagta aaagaatcgt gtaaagactt catggatatt ttatctcccg ttccatttat
     1201 gaatgcactt cttaaaaatg agacggtaga attgcagtat cagatttatc caaattctaa
     1261 tggatatgaa tctttatacg caagagcaat tcgtctatat gatgaaaaac accattttcg
     1321 ttttgtgatg ggatttcgtc ctatggatga ggttcgtaaa aaggaaaatg ttttagaact
     1381 tcaaagagag attattgaag ggattagtaa ggattatttt tctgtacttc ttgtggattt
     1441 ggagaatggt caaatctatt cgtatcgtga agcgggtagt aatggtaagc gtattgcgga
     1501 tttttgtcgg tcttatcatg atcgttggac tgaacttttg ccggcttatg ccaaagattt
     1561 ggtttcggat gcttcaagag aaaatttttt agaagaactt tcctttgaaa aactgaaatc
     1621 gaccaatgaa gattattctt ttacttatga atatatgtcg gatgagggtg ttttatatta
     1681 ccaggctcgt gtttcttatg taaagaaaaa ggatcgtaca agagcggttg tgattggtac
     1741 aagagatatt gatgatttga ttcaaaagga gcgtcttcaa aaggaacagt taagaaaggc
     1801 ttatattcaa gctgaaaatg caagtaaggc caagacggat ttcttaaata gtatgtcaca
     1861 cgatattcgt acgcctatga atgtaattct tggatataat caattgatga aaaaaaagct
     1921 ttcagatcct gttttggtgg attatcaaaa gaagattgag cagtcgggga atttattact
     1981 ttccattatt aatgatgtat tgaatatggc tcgtattgaa agtggtaaga gtgtgttagt
     2041 tgaaagtagt gagtccattg tggatatggt tgaagaagta tcgaatgttt ttggtgctat
     2101 ggccaaagaa aaagagattg tattcgatac aaaaatggat ataaaacata aacaagtatt
     2161 gtgtgattgt acgaaggtga aggaaatttt ggtgaatttg attggcaatg ccattaaata
     2221 tacaccaaat ggtggtcatg tcacagtgga cgtaaaggag cttgagtgtt caaaagaggg
     2281 ttatggtaca taccaaattc gaatcataga tacgggtatt ggaatgagtg agagttttat
     2341 tccaacccta tttgatccat ttacgcgtga agcaacaggt gacattattg gtacagggct
     2401 tggtatgcct atcgtaaaaa aactagtaga tatgatgggt ggtacgattg atgttgagag
     2461 taagcttggt aagggcagta cttttacaat aacgatcgat cataagcttg gaaataaaga
     2521 aaaagataaa gtgaaacaag agaagcttgt tgattctaag cattctttag aaggtaaaca
     2581 tgtattacta gcagaagata atgaattgaa tgcagaattt gcgaagatta tattagagga
     2641 ttgtggctta catgtagact tggcaagaga tggtttagaa tgtgttagta aagtgattca
     2701 aaatccggtt ggtacgtatg attttatttt aatggatatt caaatgccaa atatgaatgg
     2761 gtatttagct acccaaaaaa tcaggtcgat tccagaaaag aatacgattc caattattgc
     2821 gatgagtgcg aatgcgtttg atgaagataa aaagaaggca cttaaagcgg gtatggatgg
     2881 atatatttct aaaccaattg atatggaaaa gcttcaaagt caaattatag agatattaaa
     2941 agagaaagcg ggcagttaaa atgtccgttt taattataga aaaagagttg aacataagtt
     3001 tgtggggggc ttagttcaac tcttatttag gaaactggat atgcaaatta caagcaatcc
     3061 agcaataagt attatagcac tatatgttta aagagacaaa gttataacat tttctccgca
     3121 atatgataca atgaaaatga taacaggggt gagtaagatg tgttgtgatg gtgtcataca
     3181 atatttagaa aaagaaaagg tttctatact tataaaagat cgacatacat gtttagattt
     3241 gaatgatagt aatatgtatg ttttaaagaa gggtattgtt aaagtgagtt tgatcatgca
     3301 agatgagcgt gaatttaata ttacctattt acaaggtccg gatgtggtga gcttgtattg
     3361 tgattgtttg acacaattta gagattgcct tccaaagatt cgtgttgaaa gtgaacaagc
     3421 tgaattatat tgtatttcaa aagaaaaatt ctataagcga gttaaagaag atgcgaatct
     3481 tcaaaattat gtaaatacat actatcgcaa tcgattaaaa atagccatca caagagaaca
     3541 agtgatgatc atgaattcaa aggcaggtgc ggtatgttct tggttatatc gtttggcgag
     3601 tttatttggc attcaaacac aaaagggtat atttattgat ttgcaggtca caaatgagga
     3661 tattgcgaat ttttgtggga ttacaacacg taatagtgtg aatcgagtga ttcgtggttt
     3721 aaaagaaaag catatcattc aagtaaagaa taatcgaatt attgttttag atatggacta
     3781 tttaaagcaa ttcacaaagt agggtagtgt gaaactatcc ttttttaatg ttaagaaaat
     3841 attaaaacaa actatcttta gttatatata aagaattaat tgaagtaaat aattcgtttg
     3901 cacaaattaa cagagtgatt tgcacaaatt aatcgacttt atataaagga gaaatgaatg
     3961 tatataacca aaaatgttaa aatatgtgat aaataaagga aaataccaca tatcaaagcg
     4021 ttaaattagc taaccagtga acaaaagtta taaaaagttg tttgaaaacg tttacaggaa
     4081 agagtatatt atatgaacag aaatgcgttc ttgttaggtt gtttataaca gaaaggttag
     4141 attagcttaa gataaaggga aaatgaatat atcaaccaat taacataaga tgatgtatgt
     4201 ttatcttaac ataatcttaa tgtgtagata gagatatgga aaaaagggat ttaagacatt
     4261 taaagcgaaa agagttgatt gacctcattg ataagatgca aacagaaagt gatcttgtat
     4321 caaatgttga ggttaaagaa gagcttggtc gtttaaaaga gagagaaaag tatttaaagc
     4381 atttacgtaa gactttgagt gtactcgttg ttgtggctgc catttctgtg ttggtcgcaa
     4441 cactgtggat gcctgtatta aagatttatg gttcgagtat ggatccaact ttagaaaatg
     4501 ggcaggttgt ggtgtctata aagacaaaga aattaaagtc tggagatgtt gtagcatttt
     4561 ggcagggcaa taagttgttg gtgaagcgtg ttattgcagg tcctggtcaa aaggtagata
     4621 tagatgtgaa tggaaaggtg tctgtggatg gtaaagctat tcatgaaact tatttggata
     4681 gtgagtcatt aggtaataca gacattgatt ttccccatca agtcgaagat tctcgttggt
     4741 tttgtatggg agataatcgc gagagttcga tagattctag aagtgctgca ataggggata
     4801 tttcaaaaga acaaattgag ggtaaagtct tattttcagt ttggcctttg aacaaaatag
     4861 gtattgtgaa ataaacagaa agtaggtgtg attgtgcaag agtatttaag gcgtttatta
     4921 aaagacaaag cgcgcttaag aaaatggaaa aggatcatga tagcactttc ttgtattgtg
     4981 gttgtttgta cggtctatgc attatcttta ccagctcaaa cattggcttg tgataaggaa
     5041 gaacatacac atacggcaga gtgttatgat gaaaacaatg aattgatttg cgaaaaagaa
     5101 gagcatacac ataatgagga ttgtaacaaa caagaagaag tagcaaatga tgagccagaa
     5161 actcaaaaca aggatgaaca agtatctcaa gtaagtgaag aagaagtaga ggcaacaacg
     5221 actacaacta cagaaactac aacagaaact acaaaacaac cttttgattt aagctaccac
     5281 actgaaaata ttgaatctgt aaagtttgtt tataagaaga cagaaattat tgatggtaaa
     5341 gaaaagacta cggaatatga tgttccaaca gatggtagta ttctttcata tgatacttta
     5401 gatatgacat acaaattatt ctttaaaggt atttccgcaa agactttgaa agagtgtggt
     5461 ggtatcatca tgtatcaact tcctaatgga ttcaaaatta gaaagatgat agagggtaag
     5521 actattttat cgggtggtgt agttattgga aacatgaatg tgagtacaga tggtttagtt
     5581 acgattactt acaaccaaca atttttgtct agattacaag aaaatgaaac acttactggt
     5641 agttttgaag tggatgccca aattgatatg aacaagattg attcaagtac tggtaaagta
     5701 acaatcacaa caccaaaggg aaacattaca ttaaattatg gtctatatta taaggaacat
     5761 tatggaagtg ttagtgtaga taagagttgc aaaaaagaag ctactagtga ttatataaaa
     5821 tatacgatta cattaactgc gggtgatgat ggttgttgtt acacatgaaa tgggttttgc
     5881 cagaaaggta gggacaaaac ccatttcatg tg
//
LOCUS       DBMDWL010000129         5908 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5300_length_5908_cov_2.425521, whole genome shotgun sequence.
ACCESSION   DBMDWL010000129 DBMDWL010000000
VERSION     DBMDWL010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5300_length_5908_cov_2.425521"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            45..887
                     /locus_tag="ACZPDN_08700"
     CDS             45..887
                     /locus_tag="ACZPDN_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1298745.1"
                     /translation="MFYKVKESFSKAAKILHNNQPNISRTMNNIESEFGCKLLVRSHS
                     GITLTPEGEQLYEHVAIAIEQLYQGESEILSNKTLEHGHISIGVSETALNIFLLSKLE
                     HFHKAYPHVKIKLSNHSSIQAVDALEKGIVDIAVVTSPINIKKPLHTTSLYCFEEILI
                     GGKEYESLSNEILDIQQISHYPFITLGKNTSTYTYYSNLFLNHKIPFHTDLEVATADQ
                     ILPLVKHNLGIGFYPKELISMDSDIFEIQTNIKNPERDILLVQDASRATNIATQKFID
                     TLSL"
     gene            complement(896..1921)
                     /locus_tag="ACZPDN_08705"
     CDS             complement(896..1921)
                     /locus_tag="ACZPDN_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR1298746.1"
                     /translation="MNFVNKYGKGILVCLLIAIPSWIIGKMFPVIGGAVIAILAGMII
                     AMFWNDKQKAEAGIKWTSKIVLQTAVVLLGFGMNLGVIFETGKQSLPIILCTITTSLV
                     LAWIMKKVLKVPTNTSILVGVGSSICGGSAIAATAPVIDADDDEIAQSIAVIFFFNVL
                     AAIFFPILGKALGFDTMHGDAFGIFAGTAINDTSSVTAAASTWDSMWGLGSATLNKAV
                     TVKLTRTLAIIPITLGLSFIRAKKERQASNFSLKRAFPMFILYFILAAIFTTVCVNLG
                     VDSSVFAPLKELSKFLIIMAMAAIGLNSNVIQLIKTGGKPIAVGATCWCGITIVSLVM
                     QHVMHIW"
     gene            2024..2899
                     /locus_tag="ACZPDN_08710"
     CDS             2024..2899
                     /locus_tag="ACZPDN_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1298747.1"
                     /translation="MLDYRTKTFLTVCKTLNFTTAAKQLNITQPAVSQHIHFLEKQYN
                     TTLFAYKNKELSLTPSGEILYKHLLAMKNNEQAIMNEINNITSYIETLSIGVTMTIGE
                     YAIIDKLANFIQNHPEINIHLHYGNTSKLLELLDQGQISMAIVEGNYPKENYSHIKYS
                     TEDYIAVCATSHHFTKEPHTIHDLISEHLLVREEGSGTRNILEQSLIAHGLHISNFTH
                     YTQVENMHTIMNLLKKDCGISFLYKIAVEKELKSKELKEITLDDFKLQHDFDIIWQKE
                     SIYADKYLSISEEFI"
     gene            complement(2892..3242)
                     /locus_tag="ACZPDN_08715"
     CDS             complement(2892..3242)
                     /locus_tag="ACZPDN_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1298748.1"
                     /translation="MKATIFIAYIFLLMYFVVGVAFIYLFVLVVRALKKYIKSEPVRK
                     EKAESAKSLGEVIKRYRTQRKMTQEFVAESLGVSRQAVSKWESGASDPSTTNLMALAK
                     LFGVTAEEILKEVK"
     gene            complement(3317..3775)
                     /locus_tag="ACZPDN_08720"
     CDS             complement(3317..3775)
                     /locus_tag="ACZPDN_08720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1298749.1"
                     /translation="MKIIKVINDEEKKKITSAILEALPEWFGIVEAREEYILDSVGKD
                     FFCAMEKDKVVGFLYLKQTGKDTVELAVMGVLKEYHRKGIGRALFEYAKESVKESGYS
                     FIQVKTVKMGMYEDYDRTNQFYISLGFKEFEVLNSLWDENNPCQIYVMAV"
     gene            complement(3817..4611)
                     /locus_tag="ACZPDN_08725"
     CDS             complement(3817..4611)
                     /locus_tag="ACZPDN_08725"
                     /inference="COORDINATES: protein motif:HMM:NF040149.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5700 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HIR1298750.1"
                     /translation="MIINTEIVDAYLNGEDINPYASMFIYNQGDRNIFQGPKKDKVEV
                     VSNIKDEIINHVKDFVPCNKRIWDTLFKDWQNDLDDIILDLVVGCKEPNDAFVLKDLN
                     GRHHMIFDLLCWEKYVGKISLSKLSQNLLTHELFHVLIGKYYTDIEKSEQFGNYIDKL
                     DAISFNEGFAHLVSYNQQEINSVEWEKLEDIYIQSINKMKLALMETNPQSQEQYIYEA
                     NFGNYYDKYACMCGMIYLAKEWQLGGYARLKEVFDQGYHGFARKCI"
     gene            complement(4656..5282)
                     /gene="nth"
                     /locus_tag="ACZPDN_08730"
     CDS             complement(4656..5282)
                     /gene="nth"
                     /locus_tag="ACZPDN_08730"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR1298751.1"
                     /translation="MNANEILDEMEKIFPNAKCELYHESAFQLIVAVVLSAQTTDAMV
                     NKVTPALFKAYPTAEKMAEATVSELEPYIKRIGLYRNKARSISNLSKDLVERYHGQVP
                     YTYKDLMSLAGVGRKTANVVRSVAFDIPSFAVDTHVNRVSKRLGLAKYNDSVEKVEEK
                     LKRKIDRSRWNQGHHDFIFFGRYLCHSRNPECERCPFKSFCKKDKYEK"
     gene            complement(5279..>5908)
                     /locus_tag="ACZPDN_08735"
     CDS             complement(5279..>5908)
                     /locus_tag="ACZPDN_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIR1298752.1"
                     /translation="PRLLKVERKEKYIVDDQLDGSITIYQEDTIMADFDFNAPYVDRR
                     TWIIDHLQDLVLEPKEILVVLLIDFFNQQHISIDHELISEKLKISVDEVEDIFTELSD
                     KGYLTLDYADGSLIFNIEGIFELSKASDTSIDRSLLEQFEMEFARPLSSTEMQRIIDM
                     ASMYDERRVICALNEAVCNEACDLNYIERILQNWQQKGLSTEDIENGKR"
BASE COUNT         2049 a         1228 c          720 g         1911 t
ORIGIN      
        1 aggaaatttc aactattcat ttaaaaattc aattgtagtt atacattttt tacaaagtta
       61 aggaaagctt ttcaaaagcc gcaaaaatcc tacacaataa ccaacctaac atttcaagaa
      121 ccatgaacaa catagaaagt gaatttggat gtaagctttt agtacgctcc cattccggta
      181 ttactctaac acctgaaggt gaacaactct atgaacatgt tgcgatagcg attgaacaac
      241 tttatcaagg agaaagtgaa attctttcaa ataaaacctt agaacatgga cacatctcaa
      301 ttggcgtaag tgaaacggct ctaaatatct ttttattatc caagcttgaa catttccaca
      361 aagcctatcc ccacgttaaa atcaaattat ccaaccactc ctctattcaa gctgttgacg
      421 ccttagaaaa aggaattgtc gatattgccg tagtcacaag tccaatcaac atcaaaaagc
      481 cattacatac tacttcactc tactgctttg aagaaatatt aattggtgga aaagaatatg
      541 aaagcttaag caatgaaatt ctagacatcc aacaaatctc gcattatcca tttattacct
      601 taggcaaaaa cacaagtaca tatacctatt attcaaactt gttcttaaat cacaaaattc
      661 cttttcatac cgatttagaa gtggctactg ccgatcaaat tcttccatta gtcaaacaca
      721 atctaggcat tggattctat cctaaagaat taatttctat ggattctgat atttttgaaa
      781 ttcaaacaaa tataaaaaat cctgaacgag atattcttct tgtccaggat gcaagtcgag
      841 caaccaatat tgccacacaa aaatttattg atacgctttc tttataattc aaaatttacc
      901 aaatatgcat cacatgctgc atcactaaac ttacaattgt gataccacac caacatgttg
      961 caccaaccgc aattggcttt ccacccgttt tgatcaactg aattacattg ctattaagtc
     1021 caatggctgc catagccata atgatcaaaa atttagataa ttctttaagt ggtgcaaata
     1081 cactagagtc tactcctaaa tttacacata cagttgtaaa gattgctgcc aaaataaaat
     1141 aaagtataaa cattgggaag gcacgtttca aactaaagtt tgatgcttgt ctttcttttt
     1201 tagcacgaat aaaagatagc ccgagtgtaa ttggaataat cgcaagagta cgagttaatt
     1261 ttactgttac cgccttattt aatgtggcac taccaagacc ccacatacta tcccaagtag
     1321 aagctgcagc cgttacactt gatgtatcat taatggctgt tccagcaaaa ataccaaagg
     1381 catcgccatg cattgtatca aaacctaaag cttttcctag aataggaaag aaaatagcag
     1441 caagtacatt aaagaaaaag ataactgcga tagattgggc aatctcatca tcatccgcat
     1501 caataacagg tgccgttgcc gctatggcag aacctccaca aatcgaagaa ccaacaccaa
     1561 caagtattga tgtatttgta ggtactttca aaactttctt catgatccac gcaagcacaa
     1621 gcgatgtcgt aattgtacaa agaataattg gtaaagattg tttacctgtt tcaaaaataa
     1681 cacctaaatt cattccaaat ccaagtaata caacagccgt ctgtaaaact atcttagaag
     1741 tccatttaat accagcttct gctttttgtt tatcattcca aaacatcgcg ataatcattc
     1801 ctgcaagaat cgcaatgaca gcaccaccaa tgactggaaa catctttcca ataatccatg
     1861 atggaatcgc aattaaaaga caaaccaaaa tgccttttcc atatttattt acaaaattca
     1921 tttaaaattc cctccatgcg aagaattgta tcactataca acaataaggt aaaattatat
     1981 aattctatcg aacaataatt atttcttatc aatagaggta cctatgctag attatcgaac
     2041 aaaaacattt cttactgtat gtaagacttt aaattttaca acagccgcca aacaattaaa
     2101 tatcactcaa cctgccgtat cacaacacat ccatttctta gaaaaacaat acaataccac
     2161 attatttgca tacaaaaata aagagttatc actaactcct tctggtgaaa tactatataa
     2221 gcatctatta gctatgaaaa ataatgaaca agccataatg aatgaaatca acaatatcac
     2281 atcctacatt gaaacactat ccattggagt cactatgact attggtgaat atgcgattat
     2341 tgataaattg gcaaacttta ttcaaaacca ccctgaaatt aacatccacc ttcactatgg
     2401 aaacacatct aaactactcg aattactcga tcaaggtcaa atcagtatgg ccattgtcga
     2461 aggaaattat ccaaaagaaa actattctca cataaaatat agcacagaag actatattgc
     2521 cgtctgtgct acatcccacc atttcacaaa agaaccccat accattcacg atctaatatc
     2581 tgaacatctt ttggttcgag aagaaggttc tggaacaaga aatattcttg aacaaagctt
     2641 aattgcccat ggtttacata tttcaaattt cacacattat acacaagttg aaaatatgca
     2701 taccatcatg aatctattga aaaaagattg tggaatttct ttcctatata agatagctgt
     2761 agaaaaagaa ttaaaatcaa aagaactcaa agaaattact ttagatgatt ttaaactaca
     2821 acatgacttt gacatcatct ggcaaaaaga aagtatctat gctgataaat atctttcaat
     2881 cagtgaagaa tttatttaac ttcctttaaa atttcttcag cagtgactcc aaatagctta
     2941 gctaaagcca ttagattcgt tgtacttgga tccgatgcac cactttccca ttttgataca
     3001 gcctgtcttg aaacacctaa agactctgcc acaaattctt gagtcatttt acgttgcgta
     3061 cgatatcttt taatcacttc tccaagtgac tttgcactct ctgctttctc ttttctaaca
     3121 ggttcagatt taatatattt ctttaatgcc ctaacaacca acacaaataa gtatatgaat
     3181 gcgacaccaa ctacaaaata catcaacaaa aaaatataag ctataaatat agttgccttc
     3241 ataatacata tttcctcctt ttcaccatca ttatacggta attcattgcg gttgctctac
     3301 caactatttg tcaactttac actgccataa catagatttg acaaggattg ttttcatccc
     3361 acaacgaatt caagacttca aattctttaa aaccaagtga aatataaaat tgatttgtac
     3421 gatcataatc ttcatacatt cccatcttca ccgtttttac ctgaataaac gaatacccac
     3481 tttcctttac actttccttt gcatattcaa atagtgctct tccaatacct ttacgatgat
     3541 attcttttaa cacacccatt acagctagtt caaccgtatc tttacccgtt tgtttcagat
     3601 acaaaaatcc aacgacctta tccttttcca tagcacaaaa gaaatctttt cctacgctat
     3661 caagaatata ttcttctctt gcttctacaa ttccaaacca ctcaggcaac gcttctaaaa
     3721 tagcgcttgt gattttcttt ttttcttcat catttataac tttaataatc ttcatattca
     3781 tacttccttt actattggct aaaaattaaa tctagtttat atacactttc tcgcaaagcc
     3841 atgataccct tgatcaaaca cttcctttag tcttgcatat ccacctaatt gccactcttt
     3901 agctaagtag atcattccac acatacatgc atatttatca taataatttc caaagttggc
     3961 ttcataaatg tattgttctt gtgattgtgg atttgtttcc atcaaggcta acttcatttt
     4021 atttatggat tgaatataaa tatcttctaa cttttcccat tcaacactat taatttcttg
     4081 ttgattatag gaaacaagat gtgcaaatcc ttcattaaac gaaatcgcat ccagtttatc
     4141 tatataattt ccaaattgtt cacttttttc aatatctgta taatattttc caatcaacac
     4201 atgaaataat tcatgagtta aaagattctg cgaaagttta gataatgaaa tctttccaac
     4261 atacttttcc caacacaata aatcaaagat catatgatgt cttccattta aatctttaag
     4321 tacaaatgca tcattaggtt ctttacaacc tacaactaaa tccaaaataa tatcatctaa
     4381 atcattttgc caatctttaa acaaggtatc ccagattctt ttgttgcaag gaacaaaatc
     4441 cttcacatga tttataattt catcttttat atttgatact acctcaactt tatctttttt
     4501 aggcccttga aatatatttc tatctccttg attatatata aacattgaag catatggatt
     4561 tatatcttcc ccatttaaat aagcatctac gatttctgtg tttataatca taattttctc
     4621 ctatataaca gaaaaacact acaagtgtag tgctattatt tctcatattt atctttctta
     4681 caaaagcttt taaaaggaca tctttcacat tctggatttc ttgaatgaca aagatagcga
     4741 ccaaaaaata taaagtcatg atgaccttga ttccatcttg aacgatcgat ttttcgcttt
     4801 agtttttcct caaccttttc aaccgaatca ttatatttgg ctaaacccaa acgcttactg
     4861 acacgattga catgggtatc taccgcaaag ctaggaatat cgaaagctac actacgaacg
     4921 acattcgctg tctttcttcc aacacctgct aaagacatta aatccttata tgtgtaaggt
     4981 acttgtccat gatatctttc aactaaatct ttacttagat ttgaaatgga gcgagcctta
     5041 ttacgataca aaccaattct tttaatgtat ggctctaatt cagaaactgt agcctctgcc
     5101 atcttttctg ccgtaggata cgccttgaat aatgcaggtg ttactttatt caccatcgca
     5161 tccgttgttt gtgcactaag tacaaccgca acaatcaatt gaaatgcaga ttcatgatac
     5221 aattcacact tggcattcgg aaagattttt tccatttcat ccaaaatttc attcgcattc
     5281 atcgtttacc attctctata tcctcagttg acaaaccttt ttgttgccaa ttctgtagaa
     5341 ttctttcaat ataatttaaa tcacaagcct cattacatac agcttcattt aatgcacaaa
     5401 tcacacgacg ttcatcatac atacttgcca tatcaataat tctttgcatt tccgttgaag
     5461 atagtggacg tgcaaattcc atctcaaact gttctaataa agaacgatca atggaagtat
     5521 cactggcttt agaaagttca aagatacctt caatattaaa aatcaaagat ccatccgcat
     5581 aatctaaagt caaataccct ttatctgata attcggtaaa gatatcttcc acttcatcca
     5641 cactaatctt tagtttttca ctaatcaatt cgtgatcaat tgaaatatgc tgctggttga
     5701 aaaaatcaat caacaatacg actaatattt cttttggttc tagaacaaga tcttgtagat
     5761 gatcaatgat ccaagttcta cgatccacat aaggtgcatt aaaatcaaaa tcagccatta
     5821 ttgtatcttc ctggtatatt gtaattgacc catctaactg gtcgtctaca atatacttct
     5881 cctttctttc aacctttaat aaccgtgg
//
LOCUS       DBMDWL010000130         5846 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5398_length_5846_cov_2.463185, whole genome shotgun sequence.
ACCESSION   DBMDWL010000130 DBMDWL010000000
VERSION     DBMDWL010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5398_length_5846_cov_2.463185"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="ACZPDN_08740"
     CDS             <1..196
                     /locus_tag="ACZPDN_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298753.1"
                     /translation="ESNISYKPFWGICRPLNSWRANSPNPIQIYCAFFTESAYFLNNS
                     FYSYDFLIVLIEAYSISFIE"
     gene            complement(1181..3322)
                     /locus_tag="ACZPDN_08745"
     CDS             complement(1181..3322)
                     /locus_tag="ACZPDN_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1298754.1"
                     /translation="MRIKNCYYHILIALLGVCMIFSSAFYFIQTQKDETEAKLVKIVN
                     YVKVQCSTYTHYNEASESKSLLRTIESCRQLQTNIQSEVDAGNPIDQAFLKENLSTLW
                     MDGSLVLDENGNIESSYSTNDSILKQVHKYIRKDIVLDYMNYPERTYSQRIELDDGSH
                     IDIATCARSDAPGIVATYFYTSAYFAQNYNLTLQNLLKGYSTKSDGTILVCDKGNIIA
                     SNDRSLLYASTKNNEVVQFLKKNKDSQHIYRFNSKGAGYYGIMLKQSNYYIFAYIPDH
                     VVFEGVFTKLVAILLIYILFVLVLRYIGYRNNRIRQVKDQEKEKAHQEELLKAAKKAE
                     AANVAKTEFLQRMSHDIRTPINGIIGMVEVGDYYRDDIKKQADCRAKIKDASNTLLEL
                     VNEVLDMSKLESGEIVLEEVGFNIDKLSDETIGIVEELAKERNIQIVEEEKSITHPYL
                     MGSPIHVKRVLMNVLSNAIKYNRDNGFIYISYRELESKEPSHVIIEFICRDTGIGMSK
                     EFQDKIFEPFAQEHMGSRSKYAGTGLGMPIAKNLVEKMGGTIEFTSQEGVGSQFVIRI
                     PFKIDEEHKNRKAKESASASIEGLRVLLVEDNELNMEIARFIIENEGARVTCATNGKE
                     AVDIYKNAPESFDIILMDIMMPEMDGYEATRMIHEMDLNIPIVAMTANAFMEDKIKAK
                     KAGMNAHVSKPLDKDILIRVISKLCKKDRVR"
     gene            complement(3312..4115)
                     /locus_tag="ACZPDN_08750"
     CDS             complement(3312..4115)
                     /locus_tag="ACZPDN_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR1298755.1"
                     /translation="MKGKRIITSLLSLSLLVCLLSGCKTSDLESDESNLPVITLGSDN
                     YPPYNYLNEDGNPTGIDVDLASEAFKRLGYRVEVVYIDWEKNKELLESGKIDCIMACF
                     SMEGRLDDYKWAGPYISSRQVIAVNKDSDIYTLDDLKGKNVAVQSTTMPEDILLNGNY
                     NVGNLISLNHRELIFTFLQKGYVDAIGAHEESIIQYMKDYDANFRILEEPLMKVGVGV
                     AFSKNDKRDLCEKLDKTLKEMRKDGTSKKIIGKYLSNPEKYLEVDDFAY"
     gene            complement(4117..5592)
                     /locus_tag="ACZPDN_08755"
     CDS             complement(4117..5592)
                     /locus_tag="ACZPDN_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024878.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR1298756.1"
                     /translation="MKSLEERQKILIVDDSKFNQELLMEILGENYQYIVANDGLEAIS
                     ILQKDWTIDLMLLDINMPKMNGFEVLEYMNKYQWINEFPVIVISAEEGTSIIEKAYNL
                     GVVEYIQRPFDAFIIKRRVMNTLVLYANQKRLTNVVVNQVYEKEENNKIMIGILSNVV
                     GTYNKESKEHILHIRIATQMLLRRLVEKTDAYPLTESDITLIATASSLHDIGKVGISK
                     DILNKPAKLTDEEYEIMKTHTLLGAKMIRSMEYPNDNPLVQMAYEICRWHHERFDGKG
                     YPDGLVGNDIPISAQVVSVADVFDALTSTRVYKKAYDYRTAIQMILDGKCGCFNPLLL
                     ECLKEMGTDIYVAFKTQKDDYKDYHEAKKLSGEILKSETLPYNDHSQRIIDFLQEKMD
                     FFELSQSKVLFDYNPLIGELTIIDREKNKKYKKSTLEFESFDVNEKVIEKVKKHLNKT
                     SVENKDFTLQFKELNIKFHTLWSGLDTDQYIGVIGQIDRRR"
BASE COUNT         1819 a         1222 c          730 g         2075 t
ORIGIN      
        1 cgagtccaat atcagctata aaccgttttg gggtatatgc cgccccttaa attcttggag
       61 agcaaattca cctaacccca tccaaattta ttgcgccttt tttactgaat ccgcctattt
      121 tttgaataat tcattttatt cttatgattt tttaattgtt ttaattgaag catattcaat
      181 atcttttatt gaataatcct tcaatttcaa gctatgtcaa cgatctttct atttttggta
      241 taaacttggt ataaaatgag tttataattt ccaattttct ctttatataa agcattttac
      301 tataaaagga atatattccc atggcaaata aaaaacactt tcttcatagt ctaataacct
      361 cacttttata taatttatca cattaaaact gaacattcca tctaacaaaa acatcatttg
      421 cataaacatt cctttatcgg tataatacaa atacagaaag actactagat acagtggtca
      481 ctctgaatta ttttagtaaa aagacactaa taatccattg tggcgatgtg aactagtgtc
      541 ttttacattt ttctataata atgaatgtaa ttcttagtat ttctactagt gccattaaac
      601 acatggcaac atcaatatat agataaatat acataaaaca cgtcctcctg caaaaaaata
      661 gaaggatctg tcgagaactc aagcagattt ctttatttaa ttcagagggt tcaccattac
      721 tacatccaac agcactcctt attttgacgt actcccaaca ttaaaatgac gagattctct
      781 ttctcacaat gcgagaaatg aagcatcgca agtctttcag cctgactcga agttttgcat
      841 acagtctgtt tttcgagaat ttccaatcca tagggactgc agtctgtact ctcatacttc
      901 attcagcaag gcttacgcct tagacctgac tggatgaaag attctttatg ctaatgattt
      961 tattgtgtgt ccacatttta accttgttgt ggaattgtca tactatattg gtcagcatga
     1021 ttagaccaat ttggctttac atctcaagct ttcactgaat ttgccaatag atcatgtcaa
     1081 attcattaaa cataccaagc tttgacatgt catcattttt ctaaccttta gaatcatatc
     1141 tcttatttta ttaatactat cgttatctaa tgttcgacaa ttatctaact ctatcttttt
     1201 tgcataattt cgatataact cgaatcagta tatccttatc taaaggcttt gatacatgtg
     1261 cattcattcc agctttttta gcctttattt tatcttccat aaacgcattg gctgtcatcg
     1321 ccacaattgg aatatttaaa tccatttcat gaatcattcg agttgcctca tatccatcca
     1381 tttccggcat cataatatcc attaagataa tgtcaaaaga ttcgggtgcg ttcttataga
     1441 tatccaccgc ttctttacca tttgtagcac aagtaactct agccccttcg ttttcgataa
     1501 taaacctggc aatttccata ttcaattcat tatcttctac aagtagaact ctaagtcctt
     1561 caatcgatgc agatgctgat tcttttgctt ttctattttt atgttcttca tcgattttaa
     1621 atggaatgcg tatcacaaat tgagaaccca caccttcttg acttgtaaac tcaatcgttc
     1681 cacccatctt ttcgacaagg ttttttgcga ttggcatacc caaaccagta cccgcatatt
     1741 tagaacgact tcccatatgt tcttgggcaa atggttcaaa gattttatcc tgaaattctt
     1801 tagacatacc aatacctgta tcacgacaaa taaattcaat aatgacatgg gatggttctt
     1861 tcgattcaag ttccctatag cttatataga taaatccatt atcccgatta tatttaatgg
     1921 cattggaaag aacattcatt aatacacgct ttacatggat tggacttccc atcaaatatg
     1981 gatgggtaat acttttttct tcttcgacaa tttgaatatt acgctctttt gcgagttctt
     2041 ctactattcc aatcgtttca tcactcaact tatctatatt aaatccaact tcttcaagga
     2101 cgatttcacc cgactcaagc ttactcatgt ctaatacttc attcactaat tcaagtaatg
     2161 tatttgaagc atctttgatc tttgcacgac aatccgcctg ctttttaata tcatccctat
     2221 aataatcacc aacttcaacc ataccaatga ttccattgat gggagtacga atatcgtgag
     2281 acattctttg aagaaattcg gttttggcaa cattcgccgc ctctgccttt ttagctgcct
     2341 ttaataattc ttcttggtgc gccttctctt tttcttgatc tttaacctgt cttatacgat
     2401 tattgcgata cccaatatat cttagtacaa gcacaaacaa tatatatata agtaaaatcg
     2461 caactaattt ggtgaaaaca ccttcaaata ccacatgatc tggaatatag gcaaagatat
     2521 aataattact ttgtttcaac ataatgccat aatagcctgc gcctttacta ttaaaacgat
     2581 aaatatgttg actatcctta ttctttttta agaattgaac gacttcattg ttttttgtcg
     2641 aagcataaag tagtgatcta tcattactcg caatgatgtt tcccttatcg caaacaagaa
     2701 tagttccatc cgattttgta ctatatccct ttaatagatt ttgaagcgtt aaattataat
     2761 tctgcgcaaa ataagcggaa gtataaaaat aagtagccac aatgccaggt gcatcacttc
     2821 ttgcacatgt cgcaatatct atatgagatc catcatccaa ttcaatacgt tgagaataag
     2881 ttctttctgg ataattcata tagtccaaaa caatatcttt acggatatat ttatgaactt
     2941 gttttaaaat ggaatcattt gtactatagg aactttctat atttccattt tcatccaata
     3001 caagacttcc atccatccat aacgtactca gattttcttt tagaaaagct tgatctatag
     3061 ggtttcctgc atccacttca ctttgaatat tcgtttgaag ctgacgacaa ctctcaatcg
     3121 ttcgtaacaa actcttcgat tcactcgcct cattataatg ggtataagtg gaacattgta
     3181 cctttacata atttacgatt tttaccaact tggcctctgt ttcatctttt tgtgtttgaa
     3241 taaaataaaa tgcggaagag aaaatcatgc aaacgcctag tagtgctatc agtatatggt
     3301 agtaacaatt tttaatacgc aaaatcatcc acctccaaat acttttctgg attcgataga
     3361 tatttaccta tgattttttt agaagtgcca tccttacgca tctcttttaa agtcttgtct
     3421 aacttctcac aaagatcacg cttatcattt tttgagaaag ccacaccaac gcctactttc
     3481 atcaatggct cttctaaaat acgaaaattc gcatcatagt ccttcatata ttgaatgatt
     3541 gattcttcat gagcgccaat cgcatccaca tatccttttt gtaggaatgt aaaaatcaat
     3601 tctctatggt ttaaactaat caaattacct acattataat tgccatttaa taaaatatct
     3661 tctggcattg tggtcgattg tacagctaca tttttacctt tcaaatcatc caaagtataa
     3721 atatcactat ccttattgac cgcaatcacc tgacgacttg atatatatgg accagcccac
     3781 ttgtagtcat ctaatcttcc ttccatggaa aaacatgcca tgatacagtc gatttttccg
     3841 ctttctaaaa gttccttatt cttttcccaa tcaatataaa ccacttcaac tcgatatccc
     3901 aatcgcttaa aagcttcact tgccaaatcc acatcaatac cggtaggatt tccatcctca
     3961 ttcaaatagt tgtatggagg ataattatca ctacctaaag taataacagg taaattggat
     4021 tcgtccgatt ctaaatcact tgtcttacat ccgcttagta aacaaacaag taaagataga
     4081 ctcaataaac ttgtaattat tcgtttccct ttcaaatcat ctcctcctat ctatttgacc
     4141 aatcacacca atatattgat ctgtatccaa accagaccat aacgtatgga attttatatt
     4201 taactcttta aattgtaaag taaaatcttt attttccaca gacgttttat ttagatgttt
     4261 tttaactttt tcaatcactt tttcattcac atcaaacgac tcaaattcca acgtactctt
     4321 tttatacttt ttatttttct ctcgatctat aatggttaac tcaccaatta atggattgta
     4381 atcaaataag actttagatt gactcaattc aaaaaaatcc atcttttcct gtaagaaatc
     4441 aatgatacgt tgcgaatgat cattatatgg caatgtttcc gattttaata tttcaccaga
     4501 caattttttt gcctcatgat aatccttata atcatccttt tgcgttttaa aagccacata
     4561 aatatctgta cccatctctt ttaaacactc tagtaacaac ggattgaagc atccgcactt
     4621 accatccaaa atcatttgaa tggccgttct ataatcatag gcctttttat atacacgcgt
     4681 acttgtcagt gcatcaaata cgtcggcaac cgatacaact tgggcagaaa taggaatatc
     4741 attacccact aaaccatccg gatagccttt tccatcaaag cgttcgtggt gccatctaca
     4801 aatttcataa gccatttgaa ccaatggatt atcattcgga tattccatac tacgaatcat
     4861 ttttgcgcca agtaaagtat gtgtcttcat aatttcatat tcttcatccg tcagctttgc
     4921 aggcttattt aaaatatcct ttgagatacc caccttacca atatcatgta aagacgatgc
     4981 cgtcgcaatc aatgtaatat cagactccgt taatggatac gcatccgttt tctcaactaa
     5041 ccttcttaaa agcatctgcg tagcaatgcg aatatgaaga atgtgctcct tactctcctt
     5101 attatatgta ccaacgacat tactcaaaat tccaatcatg atcttgttgt tttcttcttt
     5161 ttcatacacc tgattcacta ctacattggt caatcgtttt tgattggcat ataaaacaag
     5221 tgtattcata actcttcgtt taataataaa cgcatcaaaa ggacgttgaa tatattcaac
     5281 aacccctaag ttatacgcct tttcaataat tgaagtccct tcttctgcag aaatcacgat
     5341 taccggaaat tcattgatcc actgatactt attcatatat tccaaaactt caaacccatt
     5401 catttttggc atattgatat cgagcaacat taagtcaatt gtccaatcct tttgtagaat
     5461 ggaaatggcc tctaagccat cattggccac gatatattga tagttttcac ccaatatttc
     5521 catcaataat tcctgattga atttggagtc atccacaatc aaaattttct gtctttcttc
     5581 taaacttttc ataaccctat tcccacttca tcatataacc atatggtaag ttagctaaag
     5641 tatacatcta aaaaaaactt gttttcaaga acaaatgtca acattgcaca agcttttttt
     5701 ataaccaaaa aagcctggca tttatattgc caagcctata tttattgacc catcttgttc
     5761 attgccaaga tttcattttc aagcacttct ttataagaat ctaactgttt tgttctagca
     5821 ctttcatata ctttgatttc ttcact
//
LOCUS       DBMDWL010000131         5733 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5575_length_5733_cov_2.203125, whole genome shotgun sequence.
ACCESSION   DBMDWL010000131 DBMDWL010000000
VERSION     DBMDWL010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_5733_cov_2.203125"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="ACZPDN_08760"
     CDS             complement(<1..363)
                     /locus_tag="ACZPDN_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HIR1298757.1"
                     /translation="MLETPVLLLENFDEESQMLYQSFRTSGFDGPVFTFSDEGFLPDD
                     VTSIYTYYCGKKEGGKARYFNQIDVPDYWEIKASNSGGQVFDLNHERGKIFFASPLHK
                     RLVKIVDWYDDTHVVRVSD"
     gene            complement(357..1838)
                     /gene="gtfA"
                     /locus_tag="ACZPDN_08765"
     CDS             complement(357..1838)
                     /gene="gtfA"
                     /locus_tag="ACZPDN_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HIR1298758.1"
                     /translation="MIYNFNLGIGWASSGVEYAQIYRARMFRNIGVDAKFVFTDMFPS
                     ENMEHMTKNIGFLDSEVIWLYTFFTDFKTAPVTYSLSDLEKTFTDLNYTKTREGKICR
                     YVFGGSNNFYTAYMVNDHDDFVHRVELVSNGFLIRKDYFTYGRVYSEYYAPLDNKAHL
                     YHRRFFNENGSVAYEEIIDDDRVMYKFKDKILCSKEEFVGYMVSKLNLTSDDVVLIDR
                     STDIGQSILMNCKPARVGTVVHADHFSEKDTNDDYILWNNFYEYEFNMHKHIDFYICS
                     TQAQSDLMVQQFEKYYGVTPCVYTIPVGSLPELKYPKENRKPFSLITASRLASEKHVD
                     WICKAVIQARAEVPEVSLDIYGVGGEREKLETIIRDNNASDYIHLMGQHDLSEVYQNY
                     EAYIAGSTSEGFGLTLMEAVGGGLPIIGFDVRYGNPTFIRNGENGYLIDLPEEDKDKI
                     EALRKGIVDLFKQDLEKCHSVSYEVAKDFLTCNVQEKWREAVC"
     gene            complement(1857..4166)
                     /gene="secA2"
                     /locus_tag="ACZPDN_08770"
     CDS             complement(1857..4166)
                     /gene="secA2"
                     /locus_tag="ACZPDN_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HIR1298759.1"
                     /translation="MNKIDKRLHFLLGKVKEECKKVKELSDYELKNKTLEFKERLNNG
                     ETTDDLLPEAFAVCCEADYRVLGMFPYDVQILGGIALHLCYLCEMNTGEGKTLTATMP
                     MYLNGLTQKSSILVTANEYLAIRDAEEMGQVYKFLGLSVKAGVKRDTNEKLDNDSKKD
                     VYNADIVYTTHGALGFDYLLNNLVQSKEDRFMRDFYYVIIDEADSVLLDSASMPLVIS
                     GSPRVQSNLYGITDFFVTTLVEDVHYEIEDKKVWLTNKGIEYAKRYFRIENLYSKENF
                     DVLRHIVLALRAHFLLEKDIDYVATDEGEIVLLDKSTGRKMKGMKLRGGSHQAIEEKE
                     HLKLSQEQRSVASVTYQNLFNLFPKMSGMSGTIKDGKEELLEVYNKRVVVIPPRKPMI
                     RKDMPDKYFKTSEEQFDAVIQETVKRHKTGQPVLLIASLISDTEMLSKLLVQENIEHS
                     VLNANNAFWEAEIIKEAGQKNVVTVATSMAGRGTDIHLGPGVKELGGLCVLGIGRMNN
                     TRDERQARGRAGRQGEPGVSIFYTSLEDDVCTILGEDFLEKYIDKGRYISKRRIKKLI
                     NRSQKIKDESSVSQRKSSVDYDSVMKRQRTIMYKTRNDLLDGKSLDENVLIQIYKQNI
                     DDYVKENKKMDDKTMYRYILDNLSYRLKELSVSSANKKEYLKQYADMAFKDKKQLLGN
                     RFSEYCRLCTLKALDDGWIEEVDYLQQLQAAISGRSTAQRNLLYEYQREARLSFRDME
                     KSIKKAMIRNILLGEVSFGKDNEMIILYP"
     gene            complement(4168..4599)
                     /gene="asp3"
                     /locus_tag="ACZPDN_08775"
     CDS             complement(4168..4599)
                     /gene="asp3"
                     /locus_tag="ACZPDN_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HIR1298760.1"
                     /translation="MKNKSWKVVWNEDTFGTYLYGSKIWFHSREDIEFENELMPPGTV
                     IKEWYSKVNYQMMRTEPSLPIIDGESSYHIQVNMSDFESYLIRMVFYDRYENEAGTLI
                     IREPEMDFKCPLKTYSYRVQLINAGGTKMHFHSFVITEKEG"
     gene            complement(4589..>4866)
                     /locus_tag="ACZPDN_08780"
     CDS             complement(4589..>4866)
                     /locus_tag="ACZPDN_08780"
                     /inference="COORDINATES: protein motif:HMM:NF028239.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HIR1298761.1"
                     /translation="MDVLNHRFWDLFDQTDWSQTKFIISYMLEDDYDMTAYNRLISHI
                     DDVGVEVIGKGIHGRHNDDTYSIVAWFKNQYDDILKSDFNRGEPNEE"
     assembly_gap    4867..4876
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4877..>5733)
                     /gene="asp2"
                     /locus_tag="ACZPDN_08785"
     CDS             complement(<4877..>5733)
                     /gene="asp2"
                     /locus_tag="ACZPDN_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HIR1298762.1"
                     /translation="SQSFKGSIFWNGFSGVELEGDYGDDFYQIAYWRYNIPLFKGQTL
                     DLWLEYEKDDSVSIELKIEQFEGGSLSSLQNRWIYSEKDLSSIVHLSNDKIDGPVFIS
                     LLAKGRGKLKIVGLHDRHSRKEYGSFLPGGIRKVTSKREEVFMYFDPGDMKPPLNVYF
                     SGYKTQEGFEGFYMMRNMGAPFLLISESRLEGGAFYLGDKEYENLIVSGIQDCLKQLG
                     FDRSQLILSGLSMGTFGALYNGCKLRPHAILLGKPLASLGDIARNERISRPGGFPTSL
                     DVLSKNCGD"
BASE COUNT         1908 a         1267 c          661 g         1887 t
ORIGIN      
        1 atcacttaca cgaacaacat gcgtatcatc ataccaatct acaatcttca caagtctttt
       61 atgtaaagga gatgcgaaga atatcttacc tctttcatga ttcaaatcaa agacttgacc
      121 acctgaatta ctcgccttga tttcccaata atccggtaca tcaatttgat tgaaatagcg
      181 agcttttcct ccttcttttt taccacaata atacgtataa atgcttgtca catcatctgg
      241 taaaaagcct tcatcactaa aagtgaaaac cggaccatca aagccagaag ttctaaagct
      301 ttgatacaac atttgactct cttcatcaaa attttctaat aataaaactg gagtttctaa
      361 catacagctt ccctccattt ttcttgtacg ttacaagtta agaaatcctt agctacttca
      421 taagatacag aatgacactt ttctaaatct tgtttaaata aatccacaat accttttctt
      481 aaagcctcaa tcttatcctt atcctcttct ggtaaatcaa tcaaatagcc attttcccca
      541 tttcgaataa aagtgggatt tccataacga acatcaaaac caataattgg aagtcctcca
      601 ccaaccgctt ccattaaagt cagtccaaaa ccttcacttg tacttccggc aatataagcc
      661 tcgtagtttt gatacacttc actcaaatca tgttgaccca ttaaatgaat ataatcagaa
      721 gcattattat ctctaatgat agtttcaagt ttttctcttt cgccaccaac accataaata
      781 tccaaagata cttctggcac ttcagcacga gcttgaatta cagccttaca aatccagtct
      841 acatgttttt cactagccaa acgacttgcc gtaatcaatg agaaaggttt tctgttttct
      901 tttggatatt tcaattctgg taaggatcca actggaatgg tatatacaca aggagttaca
      961 ccataatatt tttcaaattg ttgaaccatc aaatcactct gtgcttgtgt tgaacaaata
     1021 tagaaatcaa tatgtttatg catattaaat tcatattcat agaaattatt ccataaaata
     1081 taatcgtcat ttgtatcctt ttcactaaag tgatccgcat gtacgactgt acctactctt
     1141 gcaggtttac aattcataag aatggattga ccaatatctg tagaacgatc aattaatacg
     1201 acatcatcac tagtaagatt taattttgat accatatatc ccacaaattc ttcttttgaa
     1261 caaagaatct tatccttaaa cttatacatc acacgatcat catcaatgat ttcttcatag
     1321 gcaacactac cattttcatt aaagaaacga cgatgatata aatgagcctt attatctaat
     1381 ggcgcataat attctgaata cacacgtcca tatgtaaaat aatcctttcg aatcaagaat
     1441 ccattggaaa ccagttctac acgatgcaca aaatcatcat gatcatttac catatatgcc
     1501 gtataaaaat tattacttcc accaaataca taacgacaaa tcttgccttc tctagtttta
     1561 gtataattta aatccgtaaa tgtcttttct aaatcactca acgaatatgt aactggtgct
     1621 gttttaaaat ccgtaaagaa agtatataac caaataactt ccgaatctaa aaagccaata
     1681 ttcttcgtca tatgttccat attttcagaa ggaaacatat ctgtaaatac aaacttggca
     1741 tcaacaccaa tattacgaaa cattcgagca cgatagatct gggcatattc aacaccacta
     1801 cttgcccagc caatgcctaa attaaaatta tatatcataa tcaagaactc ctttctttaa
     1861 ggatacaata taatcatttc attgtcttta ccaaatgaga cttctcccaa taaaatatta
     1921 cgaatcatcg ctttttttat cgatttttcc atatctctaa aagaaagtct tgcctccctt
     1981 tggtattcat ataaaagatt acgctgcgct gtagaacgac cagaaatggc tgcctgtaac
     2041 tgctgcaagt agtcaacctc ttcaatccaa ccatcatcta aagcttttaa tgtacaaaga
     2101 cgacaatatt ctgagaaacg atttcccaat aattgttttt tatctttaaa tgccatatcc
     2161 gcatactgtt tcaaatattc ttttttatta gcagaagaaa cagataattc tttaagtcga
     2221 tacgacaaat tatctaagat ataacgatac attgttttat catccatctt tttattttct
     2281 tttacataat catctatatt ctgtttataa atttgaatca agacattctc atccaaactt
     2341 tttccatcca acaaatcatt tcttgtctta tacataatcg ttctttgacg cttcataaca
     2401 ctatcataat ccactgaact ttttctttgg gaaacactcg actcatcttt aattttctgt
     2461 gaacgattga ttaatttttt gattcgacgc ttagaaatat atcttccttt atcaatatac
     2521 ttctctaaga aatcttcacc taaaatcgta catacatcat cctctaatga agtatagaaa
     2581 atggatacac ctggctctcc ttgacgtcct gcacgacctc ttgcctgtct ttcatcacgt
     2641 gtattgttca ttcgaccaat tcctaataca cataaaccac caagctcttt cacgcccgga
     2701 cccaaatgaa tatccgtacc acgacccgcc atacttgtag ctactgtcac aacatttttc
     2761 tgaccagcct ctttaataat ttccgcttcc cagaacgcat tattcgcatt caatacacta
     2821 tgctcaatat tttcttgaac caataatttc gacaacattt ccgtatccga aatcaaagaa
     2881 gcaatcaaca atactggttg tccagtttta tgtcttttta cagtttcctg aatgaccgca
     2941 tcaaactgtt cttcactcgt tttaaaatac ttgtccggca tatctttacg aatcattggc
     3001 ttacgtggag gaatcacaac aactctttta ttatagactt ctaaaagctc ctctttacca
     3061 tctttgattg taccactcat acctgacatt ttcggaaata aattaaataa gttttgatac
     3121 gtaacggaag caaccgaacg ctgttcttga ctcaatttta aatgttcctt ttcttcaatc
     3181 gcttgatgag aacctccacg aagtttcatt cccttcattt tacgccccgt actcttatca
     3241 agtaatacaa tctcaccttc atctgttgcg acataatcaa tatctttttc taataagaaa
     3301 tgggcacgta aagctaagac aatatgtctt aaaacatcaa agttttcttt actatataaa
     3361 ttttcaatac ggaaataacg cttcgcatat tcaattcctt tattagttaa ccataccttt
     3421 ttatcttcaa tttcatagtg aacatcttct actaatgttg tcacaaaaaa gtcagttatt
     3481 ccatataaat tggattgaac acgaggagaa cccgaaataa ctagaggcat acttgcacta
     3541 tctaataata cagaatccgc ctcatcgata attacgtaat aaaaatctcg cataaagcga
     3601 tcttctttag actgtactaa gttatttaac aaataatcaa atccaagtgc accatgcgtt
     3661 gtatatacta tatccgcatt atagacatcc ttttttgaat cattgtctaa cttttcattt
     3721 gtgtcacgct ttacacccgc ttttacactc aaacctaaaa acttatagac ttgacccatc
     3781 tcttcggcat cacgaatggc caaatattca tttgccgtaa ccaaaatcga acttttttgt
     3841 gtcaatccat ttaagtacat aggcatcgtt gccgttaaag tctttccttc ccccgtattc
     3901 atttcgcaca aatagcacaa atgaagtgca ataccaccta aaatttgtac atcataagga
     3961 aacataccta atacacgata atcggcttca caacataccg caaacgcttc aggaagaaga
     4021 tcatctgttg tttctccatt atttaaacgt tctttgaatt ccaacgtttt attcttcaat
     4081 tcataatcac taagttcttt aaccttttta cactcctctt tgaccttacc caaaagaaag
     4141 tgtaatcttt tatcaatttt attcatttta cccttccttt tctgtaatca caaaagaatg
     4201 aaaatgcatc tttgtgccac ccgcattaat cagttgtaca cgatagctat atgtttttaa
     4261 aggacactta aaatccattt ccggttcacg aatgatcagt gtacccgcct cattttcata
     4321 acgatcataa aataccattc gtattaaata cgattcaaaa tcactcatat tcacttgaat
     4381 atgataagaa ctctccccat caataattgg taaagatggt tctgttctca tcatttgata
     4441 atttactttg gaataccact ctttaataac cgttccagga ggcatcaatt cattttcaaa
     4501 ttcaatatct tcacgagaat gaaaccaaat ttttgaacca tataaatacg ttccaaacgt
     4561 atcttcattc catacgactt tccatgattt attcttcatt tggttcacct cgattaaaat
     4621 ctgattttaa aatatcatca tattgatttt taaaccacgc aacaatacta tacgtatcat
     4681 cgttgtgtcg tccatgaata cctttaccaa tcacttcaac acccacatca tcgatatgag
     4741 aaatcaaacg attataggcc gtcatatcat aatcatcttc taacatataa gaaataataa
     4801 atttagtttg agaccaatcc gtttgatcaa acaaatccca aaaacgatga ttcaatacat
     4861 ccatagnnnn nnnnnnatat ctccacaatt ctttgacaac acatctaatg aagttggaaa
     4921 acctccaggt cttgaaatac gctcatttct agctatatct cctaaactag ccaatggttt
     4981 acctaataaa atcgcatgtg gtcttaattt acaaccatta tataaagcgc caaaagttcc
     5041 catagaaagt ccagacaaaa tcaattgtga cctatcaaaa cccaattgtt tcaaacaatc
     5101 ttgaatacca gaaacaataa gattctcata ttctttgtct cccaaataga aagctccacc
     5161 ttcaagtctt gattcagaaa tcaacaaaaa tggtgctccc atatttcgca tcatatagaa
     5221 tccttcaaaa ccttcttgcg tcttataacc actaaaatac acattcaaag gtggtttcat
     5281 atctcctgga tcaaagtaca taaagacttc ttcacgtttc gatgtaacct tacggattcc
     5341 tcccggtaaa aaagatccat attctttacg tgaatgacga tcatgtaaac ctactatttt
     5401 taatttaccc ctaccctttg cgagtaaaga aataaataca ggaccgtcta tcttatcatt
     5461 acttaaatga acaatcgaac ttaaatcttt ttctgaatag atccaacgat tctgtaagga
     5521 tgaaagtgaa ccaccttcaa actgttcgat tttcaattca atcgaaaccg aatcatcctt
     5581 ttcatattct aaccataaat ctaaagtttg acctttaaat agagggatat tatatctcca
     5641 atacgcgatt tgataaaaat catccccata atcgccttct agttcaacac cagaaaatcc
     5701 attccagaaa atactgccct taaagctttg cga
//
LOCUS       DBMDWL010000132         5599 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5765_length_5599_cov_2.575118, whole genome shotgun sequence.
ACCESSION   DBMDWL010000132 DBMDWL010000000
VERSION     DBMDWL010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_5599_cov_2.575118"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(431..1921)
                     /gene="lysS"
                     /locus_tag="ACZPDN_08790"
     CDS             complement(431..1921)
                     /gene="lysS"
                     /locus_tag="ACZPDN_08790"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR1298763.1"
                     /translation="MEKLTQQEQVRRQKMQDLIDMGIDPFGSRYDRTSNSGIIKSSYE
                     DKTKEELDELQVTVKIAGRIMTKRRQGKAGFMNIQDREGQIQIYVRKDEIGDDQYEIF
                     KKNDIGDIVGIEGTVMKTDHGQLSVRAKNYTHLSKSLRPLPEKFHGLTDVEERFRRRY
                     VDLIMNPEAKHIALTRPKIIRAIQHYLDGQGLVEVETPVMQPILGGASARPFVTHHNT
                     LNMDFYLRIATELPLKRLIVGGLEGVYEIGRLFRNEGMDAMHNPEFTTVEAYVAYSDL
                     HGMMDLIEGLFDSVANEVLGTTDITYQGTQLSLKAPFKRIHMVDAIKEACGVDFWQDM
                     SYEEAVKLAEEHDIEVEKIHNTVGHIINLFFEKYVEETIVQPTFVYGHPTSISPLAKK
                     NKKDPRFADRYELFICGHEYANAFSELNDPIDQRERFEKQLELRELGDDEANEVDTDY
                     VEALEYGLPPTGGVGLGIDRFVMLLTDQRTIREVLLFPHMKNNNSN"
     misc_binding    complement(1966..2177)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2218..2778)
                     /locus_tag="ACZPDN_08795"
     CDS             complement(2218..2778)
                     /locus_tag="ACZPDN_08795"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR1298764.1"
                     /translation="MTLNEKVTMKDLLSEGLYILMQQKPFEKITIKQICDKTGVIRGT
                     FYNHFMDKYEALEYLIHRMFYKGIEHESNPQIIKHIFQTVSDNRDFFKSCYRITGQNG
                     FEDLMSNVFKEIFRNVFTYLDVSGMDSRFTVDFLVDYYANDLLYFLKYWISRNFEPDV
                     ETYYAKIDILFKNSIKELNIRYMDKS"
     gene            complement(2789..3346)
                     /locus_tag="ACZPDN_08800"
     CDS             complement(2789..3346)
                     /locus_tag="ACZPDN_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014689.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HIR1298765.1"
                     /translation="MLIFGILSICLSLDVFVCALEQGASIRNIRFSKALVYGLIFALC
                     SSGMFLLGHGMSTYIFGRPLATLNKYVAIFVFLILGNGMVIYSFKRGKFVERLQEFDT
                     KQIIKLALIGGLDCFLVGVGCYYMNLPYLMESFVLFLIVLAGYMIHFYIGYYNGAGYQ
                     KVYYFLCGVVYLFMSLSLIFKLIRL"
     gene            3401..4477
                     /locus_tag="ACZPDN_08805"
     CDS             3401..4477
                     /locus_tag="ACZPDN_08805"
                     /inference="COORDINATES: protein motif:HMM:NF022635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mbeg1-like protein"
                     /protein_id="HIR1298766.1"
                     /translation="MDEHISLNVFMHYNKPMKNIIDYIKEYGNLTLEEYPFNNVDSLI
                     LSQISYIKFENSSIDIESEIHLLSDFENEIDTLCMDTRVPELNEELFLQFIHSLRYEA
                     ITISHLQTNFDKDNEKQFCAMTFHLPNQIDYIAFRGTDASFVGWKEDFNLCFQENIPS
                     QISALNYVNNYKTNHKLILGGHSKGANLALYATIHTHNPIYCTYNHDGPGFLTPYQTD
                     KKVFKTIPQSSVIGLLLEETNNYQIIQSDAISILQHDPFTWCVENGDFIYLKQNDQLS
                     KHTQSTLSKWLKSIDVNTRKQVIDTIYSIFSNYDNPKDFRQNIDMIEIIKSIQNVDSQ
                     TKKMIIETIQVLFKCIQNEIKRGF"
     gene            complement(4474..5100)
                     /locus_tag="ACZPDN_08810"
     CDS             complement(4474..5100)
                     /locus_tag="ACZPDN_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIR1298767.1"
                     /translation="MLKNKWFMFSLALFVIPFALNLVFYNQLPDVVPTHFDVNGQVDG
                     TMPKFMGLFGMLFLCLAIHVFTCFVSVKDPKRNNIPDFMMNILFMICPIICIVTSMTI
                     IYFGLGYSFDVGFVMNILVGVVLIVLGNYLPKVKRNYAVGIKTSWALNSDENWVLSNR
                     VGGYCMVIAGILYIILGLLGYVTMGIVVILVATIIPCIYSYILFKRGI"
     gene            complement(5087..5365)
                     /locus_tag="ACZPDN_08815"
     CDS             complement(5087..5365)
                     /locus_tag="ACZPDN_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HIR1298768.1"
                     /translation="MFEKTFKALGAPVRRDILNLLKKGKMSAGDIASHFDMSQATVSY
                     HLSLLKDAGLISEEKDKNFIFYQINASVFEEMLCYFKSFIGGNEDVEK"
BASE COUNT         2001 a         1132 c          682 g         1784 t
ORIGIN      
        1 aatgtatcgt tggattaaat atcattcaaa aagataaaaa agaaatatgg attccatttc
       61 atgaaggaag tgtgactcgt tttaatcttg attatcatca gtatgtaaaa aatgctagag
      121 aattaaaaga taaaattttg aaatatatta tcaaaacaca agaataaact aaaagtgaat
      181 aatattctta cataaaatct atatttcgtg atatactctg attgcgataa gactacgtaa
      241 tgtagtaaca gaaaagagga agctaccaac ttcctctttt tttcgggtct ttttcgggtc
      301 ctaaattcta ttttcgggtc tttaatcggg tcttttctag atgtatctgt atcaatcttc
      361 aacagtatac atccatttaa tatcctttat gtaaagcttt tattgacatt caacctattt
      421 ataacaattt ttaatttgaa ttattattct tcatgtgagg gaataataat acttcacgaa
      481 ttgttctttg atctgtcaac aacattacga aacgatcaat tccaagtcct acaccgccag
      541 ttggaggaag accatattcc aatgcttcta cataatctgt atccacttcg ttggcttcat
      601 catcacctaa ttcacgaagt tctaattgtt tttcgaaacg ctctctttga tcaattggat
      661 catttaattc agagaatgcg ttcgcatatt catgtccaca aatgaataat tcataacgat
      721 ccgcaaatct aggatctttc ttattctttt tagctaatgg agaaatggat gttggatgac
      781 catatacaaa tgttggttgt acaattgttt cttcaacata tttttcgaag aataagttga
      841 tgatgtgtcc aactgtatta tggatctttt caacttcaat atcatgttcc tcagccaatt
      901 taacagcttc ttcataactc atatcttgcc agaaatctac accacaagct tctttgatcg
      961 catccaccat gtgaatacgt ttaaaaggag cctttaatga taattgtgtt ccttgataag
     1021 taatatctgt agtgcccaat acttcatttg caacagaatc aaataaacct tcaatcaagt
     1081 ccatcattcc gtgtaaatca ctataggcaa catatgcttc aacggtagta aattcagggt
     1141 tatgcatcgc atccatacct tcattacgga ataaacgtcc aatttcataa acaccttcta
     1201 aaccaccaac gatcaaacgt ttcaatggta attcagttgc gatacgcaaa tagaagtcca
     1261 tatttaatgt gttgtggtgc gttacgaaag gtctagcact tgcaccacct aagattggtt
     1321 gcataactgg agtttcaact tccactaaac cttgaccatc caaataatgt tggattgcac
     1381 gaataatttt tggtcttgtt agtgcaatat gcttagcttc tggattcatg atcaagtcta
     1441 cataacgacg acggaaacgc tcttcaacat ctgttaaacc atggaatttt tctggtaaag
     1501 gacgaagtga ttttgaaaga tgtgtataat ttttagcacg aacagacaat tgtccatgat
     1561 ctgttttcat aacagtacct tcaataccta caatatctcc aatatcattt ttcttgaaga
     1621 tttcatattg atcatcccca atttcatctt tacgaacata aatctgaatc tgaccttcac
     1681 gatcctgaat gttcataaaa ccagccttac cctgacgacg cttcgtcatg atacgacctg
     1741 caatcttaac tgttacttgt aattcatcta attcttcttt tgtcttgtct tcataagatg
     1801 atttgataat acccgaattt gaagtacgat cataacgaga tccaaatgga tcaataccca
     1861 tgtcgatcaa atcttgcatc ttttgacgac gaacttgttc ttgttgcgtt aacttttcca
     1921 ttgttttctc ctttttcttt tatccataaa aaaaggcact tgccacaaag ggacgaagtg
     1981 ctcgtgttac caccctaatt taccagacaa atcactttgt ctagccttag tcactgtcta
     2041 taaacagcgc ttcctttaat gcaggaaata caaatagctc caagtcttta ttcccaatta
     2101 gtcctacgct ttttttcacc aaccaaaagc tctctaaagt atttctaaaa ggtactcgcc
     2161 ctcttcattg cctttttata cgcttttatc ctactagaca atacacaatt tgtcaattta
     2221 agatttatcc atatatcgaa tatttaattc tttaatactg tttttaaata aaatatcgat
     2281 ctttgcataa tatgtttcta catccggttc aaaattacga gaaatccaat actttaaaaa
     2341 gtataataaa tcattcgcat aataatccac taaaaaatcg actgtgaacc ttgaatccat
     2401 cccactcaca tctaaataag taaatacatt tctaaatatt tctttaaaca cattactcat
     2461 taaatcttca aaaccattct gtcctgtaat tctgtaacaa gacttaaaaa aatcacgatt
     2521 atcacttaca gtctgaaaga tatgttttat aatttgagga ttggattcat gctcaatccc
     2581 tttataaaac atacgatgaa tcaaatattc caatgcctca tacttatcca taaaatgatt
     2641 atagaaagtt ccacgaataa cacctgtttt atcgcaaatc tgtttgattg tgatcttttc
     2701 gaaaggcttt tgttgcatca aaatatacaa tccctcactc agaagatctt tcattgtcac
     2761 cttttcattt aatgtcatga aatcacctct acaatcgaat taatttaaaa attaatgata
     2821 agctcataaa taagtatacg actccgcaaa gaaaataata aaccttttga tatccggccc
     2881 cattataata tccaatataa aaatgaatca tatagccagc aagtacaatt aaaaataaaa
     2941 cgaaagactc catcaaataa ggaagattca tataataaca gccaactcca actaaaaaac
     3001 agtcgagtcc tccaataaga gccaatttaa taatttgttt tgtatcaaac tcttgtagtc
     3061 tttccacaaa ctttccacgc ttaaaactat aaatgaccat tccatttcca agaatcaaaa
     3121 agacaaatat cgcaacatat ttattcaatg ttgccaatgg acgtccaaaa atatatgtgg
     3181 acatcccatg tcctaataaa aacatacccg aactgcatag cgcaaaaatc aatccataaa
     3241 ccaatgcttt tgaaaagcga atattacgaa ttgaagcccc ctgttctagt gcacatacaa
     3301 atacgtctaa actcagacaa atactcaaaa ttccaaatat caacataaca atccaccctc
     3361 actaacttta ttgtatacga actttaaaaa ttttaaaatt atggatgaac atatttcatt
     3421 aaatgtattt atgcactaca ataaacctat gaaaaacatc attgactaca tcaaagaata
     3481 cggaaattta acacttgaag aatacccatt taataatgtg gactctttga ttctctcaca
     3541 aatttcctat attaaatttg aaaactcaag cattgatatt gaatccgaaa ttcatttatt
     3601 aagcgatttt gaaaatgaaa ttgatacttt atgcatggat acccgagtac ctgaattaaa
     3661 cgaagaatta ttccttcaat tcatacactc ccttcgctat gaagccatta ccatttctca
     3721 tctacaaact aattttgaca aagataatga aaaacaattc tgtgctatga cttttcattt
     3781 acccaaccaa attgactaca ttgcctttcg cggaacagac gccagctttg taggatggaa
     3841 agaagatttt aatctatgtt ttcaagaaaa tattccaagt caaatttctg ccttaaacta
     3901 tgtaaacaac tataagacaa atcacaaact catcttaggc ggacactcca aaggagccaa
     3961 tcttgccctt tatgccacta tccatacaca caatccaatt tattgtacat acaaccatga
     4021 tgggcctgga tttcttacac cttatcaaac ggataagaaa gtatttaaga ccataccaca
     4081 atcaagtgtt atcggactct tacttgaaga aacaaataat tatcaaatca ttcaaagcga
     4141 tgccatatcc atccttcaac acgatccatt tacttggtgc gttgaaaatg gtgattttat
     4201 ttatcttaaa caaaacgatc aactatctaa acatacacaa tccacactat caaaatggct
     4261 taaatccatt gatgttaata cccgcaaaca agtcattgat accatttact ctatcttttc
     4321 aaattatgac aatccaaaag attttaggca gaatatcgac atgattgaaa tcatcaaatc
     4381 cattcaaaat gtagatagtc aaaccaagaa aatgattatt gaaaccattc aagtcttatt
     4441 caaatgcatt caaaacgaaa tcaagcgagg attttaaatt cctcgcttaa ataatatata
     4501 actatagata cacggaataa tagtcgctac aagaatcaca acaatgccca ttgtcacata
     4561 accaagtaat ccaagtatta tatataagat tccagcaatg accatacaat agccacccac
     4621 acgatttgac aacacccagt tttcatcact attcaatgcc cacgatgttt taattccaac
     4681 cgcataattt cgttttactt tcggcaaata attacctagt acaatcaata caactccaac
     4741 taaaatattc atcacaaaac caacatcaaa cgaatagccc aaaccaaaat aaataattgt
     4801 catacttgtc acaatgcata taatcggaca aatcataaat aaaatattca tcataaaatc
     4861 tggaatatta tttcgctttg gatcttttac actcacaaaa cacgtaaata catggattgc
     4921 caagcacaaa aacaacatac caaataaacc cataaactta ggcattgtac catcaacttg
     4981 tccatttaca tcaaaatggg ttggtacaac atctggaagt tgattataga aaactagatt
     5041 caatgcgaat ggaatcacaa acaaagctaa actaaacata aaccatttat ttttcaacat
     5101 cttcattccc tccaataaaa gatttaaaat aacacaacat ctcttcaaat accgatgcgt
     5161 tgatttgata aaagataaaa tttttatcct tctcttcact aatcaaaccc gcatccttta
     5221 aaagcgacaa atgataagaa accgtggctt ggctcatatc aaaatgactc gcaatatctc
     5281 cagctgacat cttgcctttc tttaataagt tcaatatgtc tcttcgcact ggagcaccta
     5341 gcgctttaaa tgttttttca aacaaagcat caccacctat ttagaaatat ttctaaatag
     5401 attatatacc ttttaaatat tatttcaact ctatttagaa aattttctaa atagataata
     5461 aaaagaatca ggaagactac tctccctgat acgatgtatc cttaactttg cagaatttgt
     5521 ataactaaaa aataaaagaa gcattagtag ctgaattcac caatgcttct gtcaatatat
     5581 ttttcatcca tatcaaatt
//
LOCUS       DBMDWL010000133         5583 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5794_length_5583_cov_4.509303, whole genome shotgun sequence.
ACCESSION   DBMDWL010000133 DBMDWL010000000
VERSION     DBMDWL010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5794_length_5583_cov_4.509303"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            215..1036
                     /locus_tag="ACZPDN_08820"
     CDS             215..1036
                     /locus_tag="ACZPDN_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HIR1298769.1"
                     /translation="MKDDLKLRQSNNLILATHKMSIQQMRLFFYACSQYKGSLEIEVS
                     LDEVNRVLTEDTGNRGGTQRDRIRETIPTLMRNAIVHIEDEDGEEWCSALRKSRINKD
                     DTVVFTFDKSIQKELEELRGYTWMYLSNLTGMSSHYAVRMYEFFAMRLGSQNMKGTFD
                     FDLNKLRMFLDCTNKYARFDLFEMRVLKVAKAQINEKTNIKLKYKKIKTGRSVTSIKF
                     SFAWKSKDDVIDVEHTVHDPNDPPAGQQTIYDAGVEDEELTKEKKEAMEMLKEYD"
     gene            1048..1446
                     /locus_tag="ACZPDN_08825"
     CDS             1048..1446
                     /locus_tag="ACZPDN_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298770.1"
                     /translation="MDDDELYELAKERIANDNGKRYSQEEIMEEFEIEDDDYEEKPFD
                     WTFIEFLLSWDVFKKAYDKLLKRVKEIEPTTRDLYVYNYIYSNMGKGRTDDCLYEPIN
                     DEYDLCYSPKFKQTLIIGWSKEEIEEFKNA"
     gene            1439..1606
                     /locus_tag="ACZPDN_08830"
     CDS             1439..1606
                     /locus_tag="ACZPDN_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298771.1"
                     /translation="MPSIHSVARQQHLKQERKEKMNNQITLKQLLLVATTEDISVYLK
                     DVNCMDIERLI"
     gene            complement(1753..2904)
                     /locus_tag="ACZPDN_08835"
     CDS             complement(1753..2904)
                     /locus_tag="ACZPDN_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HIR1298772.1"
                     /translation="MSKANKSNKAIKYRLYPNDEQKVMFAKTFGCCRFVYNQLLALQK
                     QRYKDGESHLSKLKSNEFATRTLKKDYDFLKEIDKFAVSNAVFHLADAYDRFFKKQNH
                     FPKFKSKRKSKKSYTTNFTNNNILIGKNVIKLPKVGMVKAVIHKLPKDDWKLKSVTVS
                     QDSVGNYFASVLFEYEQEDIPSVSKSSTNAIGLDYKSDGLYMDSNGNKAGVHKYYRES
                     HKKLAKQQRRLSRKAGSKKNETKSSNYFKQMRKVNRIYRKIANQRLDSLHKKSTEIAN
                     RYDIVCVEDLDMKAIGNKGFGNGKATFDNGYGMFLNMLEYKLKERGKYFVKVDKWYPS
                     SQICHCCGSVKKLDLKDRVYTCDCGYIGDRDHNAAINILTEGLRILQSL"
     gene            complement(2910..3305)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_08840"
     CDS             complement(2910..3305)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIR1298773.1"
                     /translation="MELDTNNHSVFMLNYHLIMCVKYRNNVIDDAISLRLKEIFKNIC
                     LNYNISLEEWNHDKDHVHILFRGQPNSEISKFINAYKSASSRLIKKEYPEIKKHLWKD
                     MFWSQSYCLISTGGVTVDIIKEYIQTQGR"
     gene            3924..5369
                     /locus_tag="ACZPDN_08845"
     CDS             3924..5369
                     /locus_tag="ACZPDN_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013258.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HIR1298774.1"
                     /translation="MKSMSQSITKGGGNLRHNKRTQDYPTSNIDESLTPLNRTLVDIP
                     LDQMYHGLFDDAVEEYNAKQKRNDRKIDDYYHKIINDKKTKVFQELIFQVGNRDDKLG
                     VEESNELYQNVLDQFIKNNPQMKVFGAFIHQDEPKGTPHIHIDYIPVANYSRGMSKRV
                     ANNRACEQMGYKSWDDWRDKQVEMFENVCRQHGIDREVMGNKDRHMDLQSWRKLKKEE
                     ERMKEAYAKELNQTQSPLEVKNMPFLGEVVKKNDVDERERQLKSTITFQKAQISVLSD
                     ELEESKFNLNNFKNKRYVQKNKALEEENEKLKAKMSNFEREVEKEVQKRTEEYKTKAD
                     WEKSMRISLQNKVSRMSAKIENYEKVVEENEVLKKENKNLNRRIKTVTSVLYNLNYVI
                     GELFEVPQNFIVKVFNRIRKTMDQVDEQFPDVEIVKSPEDVEKANRHQRAMDRGLDNV
                     VDQTQVEINQHQEQVKLQNQRSREVFHGMER"
BASE COUNT         2014 a          926 c          979 g         1664 t
ORIGIN      
        1 ttttcttttt atatctttat tttacatttt tttttcataa taaggataat acccctacgg
       61 atttacacaa aagcccctac ggatttacac ataaagcccc tacggattta cacaataccc
      121 ctacggattt acacgagtta tccctacatt aaaaaaataa agtagttgta ttaaatccac
      181 tactatgtta aaatacatgt aggaggtatc ttctatgaaa gatgatttaa agctacgaca
      241 atctaataat ttgatattgg caacacataa aatgagcatt caacaaatga gattattttt
      301 ttatgcttgt agtcaatata aagggagttt ggaaatagag gtttctcttg atgaagtgaa
      361 tagagtttta accgaagata caggaaatcg aggtggaaca caaagagata gaataagaga
      421 aacaatccca acgctcatga gaaatgcaat cgtacatatt gaagacgagg atggcgaaga
      481 atggtgttcg gcattgagaa aaagcagaat aaataaagac gatacagttg tatttacttt
      541 tgacaaatct attcaaaaag aactagaaga actacgaggt tatacatgga tgtatctttc
      601 taatctaact ggcatgagta gtcattatgc cgttagaatg tacgaatttt ttgcaatgcg
      661 tctaggctct caaaacatga agggaacatt tgatttcgac ttgaataaac tacgtatgtt
      721 tctagattgc accaataaat acgcaagatt tgatttattt gaaatgcgtg ttcttaaggt
      781 ggcaaaagca caaataaacg agaaaacaaa cattaaattg aagtataaga aaatcaaaac
      841 gggtcgttct gttacatcaa ttaagtttag ctttgcttgg aaatcaaaag atgatgtcat
      901 tgatgtagaa cacacagtac atgatccaaa cgacccacct gcaggacaac aaactatata
      961 tgatgcaggt gttgaagatg aagaactaac aaaagaaaag aaagaagcta tggaaatgct
     1021 aaaagaatac gattaggaga gggaacaatg gatgatgatg aactttatga attagctaaa
     1081 gaaagaattg ctaacgataa tggcaaaagg tattcgcaag aagaaatcat ggaagaattt
     1141 gaaatcgaag atgatgacta tgaagaaaaa ccatttgatt ggacatttat tgagttttta
     1201 ttgagttggg atgtttttaa aaaagcctat gacaaacttt tgaaaagagt aaaagaaata
     1261 gaaccaacaa cacgagattt atatgtgtat aattacattt actcaaacat gggaaaagga
     1321 agaactgatg attgcttgta cgaacctatc aatgatgaat acgacctttg ttattctcct
     1381 aaattcaagc aaacgttaat tatcggttgg agcaaagaag aaatagagga atttaaaaat
     1441 gcctagtatt cattcagttg caagacaaca acatttaaag caagaaagga aagaaaaaat
     1501 gaacaatcaa atcaccctta aacaacttct attggtagca actactgaag atattagcgt
     1561 gtatctcaag gatgtaaatt gcatggatat tgaacgcttg atctagtgaa ctaccccgac
     1621 ttacacttcg tttagaagtc ggagcttctt gcttcaacca ctacagcctc taatgacaat
     1681 acattatagg tcttacacag tgtccacaag cgtataagtt tggctcgtcc caagcctact
     1741 tttataattt atctataagg actgaagaat cctcaatcct tctgtaagta tattgattgc
     1801 tgcattatgg tctctgtctc caatataacc acaatcacat gtataaactc ggtcttttag
     1861 gtcgagtttt tttacacttc cacagcaatg acagatttgg ctagaagggt accacttgtc
     1921 aacttttaca aaatatttac ctctttcttt aagcttgtac tcaagcatat taagaaacat
     1981 tccataaccg ttgtcaaagg tcgcttttcc attgccaaaa cctttatttc caatagcttt
     2041 catatctaag tcttcgacac atacgatatc ataccgattg gctatctcag tagatttctt
     2101 atgaagactg tcgagacgtt gattcgctat ttttctatag attctgttta cttttctcat
     2161 ttgtttaaaa tagttgcttg attttgtttc attcttttta gaacctgctt ttcgtgaaag
     2221 tcttctttgt tgtttagcaa gcttcttatg actttctcga tagtatttat gaactcctgc
     2281 tttattccca ttactgtcca tataaagtcc atcagattta tagtcaagtc caatagcatt
     2341 tgtacttgat ttggaaacag atggaatgtc ttcttgttca tattcaaaaa gaacggaagc
     2401 aaaatagtta ccgacactat cttgagagac agtaacagat ttaagcttcc aatcatcttt
     2461 aggcagctta tgaataacag ctttgaccat tccaacttta ggaagcttta tgacattttt
     2521 accaataaga atattgttgt tggtaaaatt tgttgtatac gactttttag attttctttt
     2581 gcttttgaat ttagggaaat gattttgttt tttaaaaaat ctgtcatagg cgtctgctaa
     2641 atgaaaaacg gcatttgaaa cagcaaattt atctatttct ttgagaaaat cataatcttt
     2701 ttttaatgta cgagtagcga actcatttga tttaagctta gaaagatggg attctccatc
     2761 tttgtatctt tgtttttgta aggcaagtaa ttggttataa acaaaacgac agcaaccaaa
     2821 agtttttgca aacatgactt tttgttcatc atttggatag agtctatatt taattgcctt
     2881 gttacttttg tttgcttttg acatatattt taccttcctt gcgtttgaat atattcctta
     2941 atgatatcta cggtcacccc acctgtagat atcaaacaat agctttgtga ccagaacata
     3001 tccttccata aatgtttctt gatttctgga tattcttttt ttataagtct actgcttgct
     3061 gatttataag cattgataaa tttagaaatc tcactattag gctgccctct gaacagtata
     3121 tgaacatggt ctttatcgtg attccattct tccaacgaaa tattataatt aaggcatatg
     3181 tttttaaata tctctttaag ccgtaatgag atagcatcat caataacatt attcctgtat
     3241 ttgacacaca taattaaatg ataattaagc atgaacactg aatgattatt agtatcaagt
     3301 tccatagcaa tatcctttct aaaattaagt acgactgaac gtcttttata cttataatgt
     3361 atcacagctg cacgtctaaa acaacatcta ttataaaaac tataaaagga actagacccc
     3421 tccctacact tcgcttagag gaaggggatt gcgctcctat ttttgttcaa aagacgacaa
     3481 tagaaacaac ctacatccct atctcaatta cgtggttgaa tcagtagatg aaaattggag
     3541 cattgtcata agaggtcaaa aaatagcata aggtgcagcc taaagcacct ttttattttt
     3601 tctaaaaata cggctacccg tatttttgat gccgttcagt gaaaacaaca agtagtcttt
     3661 tggatctaaa acatgcaaat gtagtacatt gtattacatc cgccctattt tctaatggaa
     3721 cgagttccat ttttctctca aaatgcgact caaagtcgca atcttttttc tattagccat
     3781 aagacttcaa aaagcgaaaa tatcatgcta tcataaaaca aagttcttgt tacttcttga
     3841 caagaagtaa cgcaatcctt attttgacac tacgtatcaa aataagttgc gccctacggg
     3901 gttgaaagag aggtagaaac ataatgaaat caatgagcca aagcattaca aaaggtggag
     3961 gaaatctaag acacaacaaa cgtactcaag attatccaac aagcaacatt gatgaaagct
     4021 tgactccgct caatcgtacg ttggtcgaca tcccactcga ccaaatgtat catgggctct
     4081 tcgatgatgc ggtcgaagaa tacaatgcaa agcaaaagcg caacgataga aagattgatg
     4141 attactacca caaaatcatc aatgacaaaa agacaaaggt tttccaggaa ctcatttttc
     4201 aagttggaaa tagggacgat aaattgggag tagaagaatc caatgaactc tatcagaacg
     4261 tgttggatca attcattaaa aacaatcctc aaatgaaagt tttcggtgcg ttcatccatc
     4321 aagacgaacc gaaaggaaca ccgcatatcc atatcgacta cattcccgta gcaaactaca
     4381 gtagaggtat gtcaaagcgt gttgcaaaca atcgtgcttg tgagcaaatg ggctataaat
     4441 catgggatga ttggagagac aaacaagtag aaatgtttga aaacgtatgt cgacaacatg
     4501 ggattgatag agaagtcatg ggaaacaaag ataggcacat ggacttgcaa tcatggagaa
     4561 aactcaaaaa agaagaagaa cgtatgaaag aagcctatgc caaagaactc aaccaaacac
     4621 aaagcccgtt agaagtcaaa aatatgccat ttctaggaga ggtcgtaaaa aagaatgatg
     4681 tggatgaaag agaaaggcag ctaaaaagta cgataacctt tcaaaaggct caaatttccg
     4741 ttttaagcga cgaactagaa gaaagtaaat tcaatcttaa taatttcaaa aacaagcgct
     4801 acgtccaaaa aaacaaggct ctcgaagaag aaaacgagaa attgaaagcg aaaatgtcaa
     4861 attttgaacg tgaggttgaa aaagaggtac aaaaacgtac tgaagaatac aagaccaaag
     4921 cggattggga aaagagcatg aggatttctt tacaaaacaa agttagtcga atgagtgcga
     4981 agatagagaa ttatgagaaa gtggtagaag aaaatgaagt tcttaagaaa gaaaacaaaa
     5041 atttgaatag aagaatcaaa acagtaacaa gcgttttgta taatctcaat tacgttattg
     5101 gagaattgtt tgaagtccct caaaacttca tagtcaaagt ttttaatcgt atcaggaaaa
     5161 caatggatca agtagatgaa cagtttcctg atgttgaaat tgtcaaaagt cctgaagatg
     5221 tagaaaaagc gaatagacat caaagagcaa tggatagagg attggataac gtagtcgacc
     5281 aaacacaagt agaaatcaat caacatcaag aacaagtgaa actccaaaat caaagaagta
     5341 gagaagtgtt tcatggaatg gaaagataga agaggttagc acctcttttt ttggttttag
     5401 gactaaaaac aagattttgt ttgagcgaaa gcgagtacct ttttttcttt ttatatcttt
     5461 attttacatt ttttttcata ataaaggata atacccctac ggatttacac aaaagcccct
     5521 acggatttac acataaagcc cctacggatt tacacaatac ccctacggat ttacacgagt
     5581 tat
//
LOCUS       DBMDWL010000134         5490 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5968_length_5490_cov_3.987753, whole genome shotgun sequence.
ACCESSION   DBMDWL010000134 DBMDWL010000000
VERSION     DBMDWL010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5968_length_5490_cov_3.987753"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(180..1505)
                     /locus_tag="ACZPDN_08850"
     CDS             complement(180..1505)
                     /locus_tag="ACZPDN_08850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR1298775.1"
                     /translation="MNKQVWIEKALGLGIQEFEIYQSTTVEKEMTWFQGQMDTLVSSK
                     VTGTSIRGIVDGKMANMALEEIDDSMCETVLKSLIEQAHIISNEETDSLRKPEPIEYV
                     KKDVTWNMASTEYVKDILQSLEAKILAYDPRVIQVGYLGYSQGSGSRSIVNSLGMDVQ
                     DADEMQYIVASVVVKEEEEIKDASLVEVVYDFEKYDLDSFVSRVCDKALNKLKATPIP
                     SCTCPVIFEKDAMTSLFAAFTGLFNGELMYKGISPLKDKFNEQIFSEQITIVDDPKNT
                     DCLSIANFDDEGCPTSSKTLVDHGKYVMMLHDTRSANRMHTKSTGNGFKSGYASSVSV
                     QPMNCYIVPGNDSFEDLCTKIKDGFVVQNLAGLHAGLDFVSTNFSLQCSGFWVKDGKR
                     DKNVTLVTVANNFLNMMKHVISVGNDLDWSYHQVVSPSIAFVECTISGE"
     gene            complement(1498..2886)
                     /locus_tag="ACZPDN_08855"
     CDS             complement(1498..2886)
                     /locus_tag="ACZPDN_08855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR1298776.1"
                     /translation="MLKKEQLEKLLERCMASSCDFAEIFEEEGTTESLSMLNGKLEDT
                     NVLIRAGIGIRLYKGVQSVYGYTNETSEESLNALVDDLRGGFGIESKSVSISLVEETH
                     DNLHPIKENPIKASLETKVALMVRASDAAKAYSDKIQKVSVGLASVCQNVQISNSEGR
                     LVHDTRIRCRMSVSSFAQDGQNLQSGYEAPGASKGLEFFEERTPESIGKEASRIALVL
                     LEAKDCPSGKMPVIIDNGFGGVIFHEACGHALEASSVSKNQSVFCNKLGQKVASDKVT
                     AIDDGTIPNAWGSQNVDDEGNPQQKRVLIENGILKSYMVDRLNGRRMGLESTSSSRRQ
                     SYKFETTSRMTNTYIAAGNDDFDEMFEGIKRGLYAKKMGGGSVNPQTGEFNFAVLEGY
                     LIEDGKISYPVKGASLIGNGADILFNIDRVGKNLERGQGMCGASSGSIPTDVGQPVIR
                     VSQITVGGTANE"
     gene            complement(2889..3578)
                     /locus_tag="ACZPDN_08860"
     CDS             complement(2889..3578)
                     /locus_tag="ACZPDN_08860"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIR1298777.1"
                     /translation="MNNIESVKGHLIVSCQALPDEPLHSSFIMGRMARAAKWGGAKGI
                     RANTVEDINEIQKEVDLPVIGIIKRVYGDCPVFITPTIKEIDELIEGAHPEIVALDAT
                     CRVRPDGRTLDELYADIRAKYPEQKLMADCSTLEECLHAAQLGFDFVGTTLVGYTEES
                     KGDKVETNDFELMRKYLEQSNVPMIAEGNIDTPEKARRVLELGCFSVVVGSIITRPQL
                     ITKRFVDCIEG"
     gene            3756..3832
                     /locus_tag="ACZPDN_08865"
     tRNA            3756..3832
                     /locus_tag="ACZPDN_08865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3790..3792,aa:Arg,seq:acg)
     gene            3855..3931
                     /locus_tag="ACZPDN_08870"
     tRNA            3855..3931
                     /locus_tag="ACZPDN_08870"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3889..3891,aa:Pro,seq:tgg)
     gene            3948..4024
                     /locus_tag="ACZPDN_08875"
     tRNA            3948..4024
                     /locus_tag="ACZPDN_08875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3982..3984,aa:Met,seq:cat)
     gene            4038..4114
                     /locus_tag="ACZPDN_08880"
     tRNA            4038..4114
                     /locus_tag="ACZPDN_08880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4072..4074,aa:Ile,seq:cat)
     gene            4126..4216
                     /locus_tag="ACZPDN_08885"
     tRNA            4126..4216
                     /locus_tag="ACZPDN_08885"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4160..4162,aa:Ser,seq:tga)
     gene            4234..4310
                     /locus_tag="ACZPDN_08890"
     tRNA            4234..4310
                     /locus_tag="ACZPDN_08890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4268..4270,aa:Met,seq:cat)
     gene            4314..4389
                     /locus_tag="ACZPDN_08895"
     tRNA            4314..4389
                     /locus_tag="ACZPDN_08895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4347..4349,aa:Phe,seq:gaa)
     gene            complement(4584..5030)
                     /locus_tag="ACZPDN_08900"
     CDS             complement(4584..5030)
                     /locus_tag="ACZPDN_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298778.1"
                     /translation="MSKKRKQEWALLKRIKLLSDKIDNLKDENKTLNNKLDSTKNELV
                     QYKNDNRLLKIRLDDVKEQNSVLQTKVNDHNHSMLTMSKKLNKSEARNIALTRFVKET
                     TRNQIHLKNGQSITVYDAIMNKLSPEDKTRVRKTMDLEGNKNSRNR"
     gene            complement(5173..5475)
                     /locus_tag="ACZPDN_08905"
     CDS             complement(5173..5475)
                     /locus_tag="ACZPDN_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298779.1"
                     /translation="MENIIMLILGVFISVVGIVNIKGNISTIHSYNRRRVKEEDIPKY
                     GKVVGTGTLIIGISLVLGFIVSFWSEKTMGYIILLAVIVGLGFILYGQFKYNKGIF"
BASE COUNT         1593 a         1212 c          829 g         1856 t
ORIGIN      
        1 ttcaaagact aagactccct tttcattgcg agaaggatct ttatcccatt gtgctggaat
       61 tagaaaatta gtcgaaataa acgcatccaa caaatttcta aatgctgaat ctgttttttc
      121 tctattaaat acaattaatg tttcttgtag tttttcattt ttaaattctt ttaccataat
      181 tattcccctg aaatcgtgca ctcaacaaat gcgatacttg gacttacaac ctggtgataa
      241 ctccaatcta aatcgttacc cacactaatt acgtgtttca tcatatttaa gaaattattt
      301 gcgacggtaa ccaaggtaac attcttatct cttttaccat cctttaccca gaagcctgag
      361 cattgtagtg agaagtttgt agaaacaaaa tcaagtccag catgaagtcc agctaaattc
      421 tgtacgacaa agccatcctt aatttttgta cataaatcct caaaactatc atttcccggt
      481 acaatgtaac aattcattgg ttgtacactt acgctagatg catagcccga tttaaaacca
      541 tttcctgtac tctttgtatg cattcgattg gcacttcttg tgtcatgaag catcataaca
      601 tattttccgt gatccaccaa cgtttttgaa gaagttggac atccctcatc atcaaaattg
      661 gcaatactta aacaatccgt attctttgga tcatcaacaa ttgtgatttg ttcagagaaa
      721 atttgttcgt taaacttatc ttttaaaggt gaaattccct tatacattaa ttctccatta
      781 aataaaccag taaaggcggc aaataaagat gtcatcgcat ccttttcaaa gatgactgga
      841 catgtacagc ttggaattgg tgtggccttt aatttattta aagctttatc gcaaacacga
      901 gaaacaaatg aatccaaatc atacttttca aagtcatata caacttcaac taaactagca
      961 tccttgattt cttcctcttc tttaacaaca acactggcaa caatgtattg catttcatcc
     1021 gcatcttgca cgtccatacc taagctattc acaatactgc gacttccact accttggcta
     1081 tagcctaaat atccaacttg aatgacacgt ggatcatagg ctagaatctt agcttctaag
     1141 ctttgaagaa tatccttcac atattctgta ctagccatat tccaagtcac atcttttttt
     1201 acgtactcaa ttggttctgg ttttcttaat gaatcggttt cttcatttga aataatatgt
     1261 gcttgttcaa tcaatgactt taaaacagtt tcacacatag aatcatctat ttcttccaag
     1321 gccatattgg ccatttttcc atctacaatt ccacgaatac ttgtacctgt cactttgctg
     1381 gatactaaag tgtccatttg tccttgaaac caagtcattt ctttttcaac tgttgtagat
     1441 tgatagattt caaattcctg aatacctagg ccaagtgctt tttcaatcca tacttgttta
     1501 ttcattggcc gtacctccta ctgtaatttg tgaaacacga atgaccggtt gacctacatc
     1561 tgtaggaatt gaacccgaac tagctccaca cataccttgt ccacgctcta agtttttgcc
     1621 aacgcgatca atattaaata ggatgtctgc accattccca atcaaagaag ccccttttac
     1681 tggatacgaa atcttgccat cttcaattaa ataaccttct aatacggcaa agttaaattc
     1741 tccggtttgt ggatttacac ttccaccacc cattttcttc gcatacaatc cgcgtttgat
     1801 tccttcaaac atttcgtcaa aatcatcatt tccagccgca atatatgtat ttgtcatacg
     1861 cgaagttgtt tcaaatttat aagattgacg acgtgaactc gaagtcgatt cgaggcccat
     1921 tcttcgacca ttcaaacgat cgaccatata actctttaaa ataccattct caattaatac
     1981 acgtttttgt tggggatttc cctcgtcatc tacgttttgt gagccccatg catttggaat
     2041 tgtgccatca tcaatagctg tgactttatc acttgcgact ttttgaccta atttattaca
     2101 gaatacactc tgatttttac tgactgaact tgcctctagg gcatgaccac aagcttcatg
     2161 gaagataact ccaccaaatc cattatcaat aataactggc attttaccag atggacaatc
     2221 ttttgcctct aataatacta atgcgatacg acttgcttct tttccaatag attctggtgt
     2281 tctttcttca aagaattcaa gacctttact tgcacctggt gcttcatagc ccgattgtaa
     2341 gttttgacca tcttgggcaa aagaggatac agacatacga caacgaattc ttgtatcatg
     2401 tacaagtctt ccttcactat ttgaaatctg tacattttgg catacacttg ccaaccctac
     2461 agatactttt tgaatcttat ccgaatacgc ctttgccgca tcacttgcac gtaccattaa
     2521 ggcaactttc gtttctaatg aagctttgat aggattttcc ttgattggat gaagattatc
     2581 atgagtttct tcaaccaaag agatcgatac agatttagat tctatgccaa atcccccacg
     2641 caaatcatcc actaaggcat ttaatgattc ttcacttgtt tcatttgtgt atccatatac
     2701 agattgcaca cctttatata aacgaattcc aataccggca cgaatcaata cgtttgtatc
     2761 ttctaatttt ccatttaaca tggataaaga ttcggttgta ccttcttctt caaagatttc
     2821 ggcaaaatca caagaggaag ccatacaacg ctctaataat ttttctaatt gttccttctt
     2881 taacatattt atccctcaat gcaatccaca aatcgttttg tgattaattg aggacgagta
     2941 atgatagatc caaccacaac actaaagcat ccaagttcta atacacggcg tgctttttct
     3001 ggtgtatcaa tatttccttc agcaatcatt ggtacgtttg attgttctaa atatttacgc
     3061 attaactcaa aatcattcgt ttcaacttta tcgcccttac tttcttctgt atatccaact
     3121 aatgttgttc ctacaaaatc aaaacctaat tgggctgcat gaagacactc ttctaatgta
     3181 gagcagtctg ccattaattt ttgttctgga tacttagcac gaatatcggc atacaattca
     3241 tctaaagttc ttccatctgg acgaacacga caagttgcat ctaaggcaac gatttctgga
     3301 tgtgcccctt caatcaattc atcgatttct tttattgtag gtgtaataaa tactggacag
     3361 tccccatata ctcgtttaat aattccaata actggtaaat caacttcttt ttgaatttca
     3421 ttaatatcct ctacagtatt agcacgaata ccttttgcgc ctccccattt agccgctcta
     3481 gccatacgtc ccataataaa agatgaatgt aaaggttcat ctggaagtgc ctgacaagat
     3541 acgattaagt gtccttttac tgattcaatg ttattcatga tttaaatctc cttcttctta
     3601 cttatatata taggtaatta tactacttct ataggtgtaa aaacacgaaa ttaaaaaaat
     3661 ttaaaaattt tttaaaaaag tgcttgcaat cattttaaag tgatggtata tttaatgggc
     3721 actgagcgag acacaaaagc tcagttgaaa taaatgcgcc catagctcaa ctggatagag
     3781 cgtttgacta cggatcaaaa ggttgtgggt tcgattcctg ctgggcgcgc catttatttt
     3841 ttttctaaaa atatcgggat gtagcacagc ttggtagtgc acttgatttg ggatcaaggg
     3901 gtcgcaggtt caaatcctgt catcccgacc attattttat ttaattcggc tgggtagctt
     3961 agttggctag agcatccggt tcatacccgg aaggtcgtga gttcgagtct cactccagcc
     4021 accaacgagt ttaacttgga cccttagctc agttggtcag agcatccggc tcataaccgg
     4081 cgggtcgtag gttcgagtcc tacagggtcc accaattata catatgcaga agtacccaag
     4141 tggctgaagg gtccggtctt gaaaaccggt aggtcgtgcg agcggcgcct gggttcgaat
     4201 cccagcttct gcgccatctt aattaattca catcgcggga tagagcagtc tggtagctcg
     4261 tcgggctcat aacccggagg tcgttggttc aaatccttct cccgcaacca tgtggtctca
     4321 tagctcagtt ggtagagcaa aggactgaaa atccttgtgt cgttggttcg attccgactg
     4381 agaccaccat ttgtcaaaga ttgcacctag catttgccag gtgctttttt tataccttat
     4441 ttaaagcata ttctcacatt atggttatat tcatcatcat taatgggagt gaatttcttc
     4501 actcttttag ataaaagtta caaaaaaact acaaaatatt ttcaaagcat attaaaaacg
     4561 gatagtaaat tatccgcttt ttttcaccga tttcgactgt ttttatttcc ctctaaatcc
     4621 attgtttttc taacccttgt cttatcttca gggcttagct tattcattat cgcatcatat
     4681 accgtaatgc tctgtccatt ttttaagtga atctgatttc tcgttgtttc tttcacaaat
     4741 cgagtcagtg caatatttct agcttcagac ttattcagct tcttactcat tgtcaacata
     4801 ctatggttat gatcattcac tttagtctgt aaaactgaat tctgttcttt cacatcatct
     4861 agtctaatct tcaataacct attatcattc ttatactgaa ccaattcatt ctttgtacta
     4921 tccagcttgt tatttagcgt cttattctca tctttcaaat tatctatttt atcactcaac
     4981 aatttgatac gtttcaacaa cgcccactct tgtttccgct tcttgctcat tctcggcata
     5041 gcgccttaag tttcaccctt cctgcaatga gcaaagcaat cggtcccccc atcgagagga
     5101 caacggagca gataactcca gctatcgcca gacctgatat tgtgttcaat tccatcttta
     5161 gctccctgat tctcaaaaaa ttcctttatt gtatttgaac tgtccataca atataaagcc
     5221 taatccgaca ataactgcta gaagaatgat atatcccata gttttttcgc tccaaaacga
     5281 aacaataaag cctaatacaa gagatattcc tataatcagt gttcctgttc caacaacttt
     5341 tccatacttc ggtatatctt cttcttttac tcttcgtcta ttgtaagaat gaattgtgct
     5401 gatattgcct ttgatattta caattcccac aacagatatg aacactccca aaatcaacat
     5461 tataatattt tccataatga cctcctaaaa
//
LOCUS       DBMDWL010000135         5486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_5979_length_5486_cov_2.262767, whole genome shotgun sequence.
ACCESSION   DBMDWL010000135 DBMDWL010000000
VERSION     DBMDWL010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5979_length_5486_cov_2.262767"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            158..895
                     /locus_tag="ACZPDN_08910"
     CDS             158..895
                     /locus_tag="ACZPDN_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1298780.1"
                     /translation="METIIQIQHLQKHFGNLKVLNDVDFDIHKGEVVTIIGSSGSGKS
                     TMLRCVNLLERMDGGHVLYHGKDILSGQMDVNEFRSKVGMVFQGFNLFNNLTVLENCM
                     LGQIRVLKKSKEEAKATALKFLDVVGMKEFANKPSTKLSGGQKQRVAIARALCMDPEV
                     LLFDEPTSALDPEMVGDVLDVMKKLADEGLTMVIVTHEMQFAREVSDRVIFMDKGVIA
                     EQGSPEQLFEHPQQERTQQFLSRYQSR"
     gene            999..2537
                     /gene="gpmI"
                     /locus_tag="ACZPDN_08915"
     CDS             999..2537
                     /gene="gpmI"
                     /locus_tag="ACZPDN_08915"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1298781.1"
                     /translation="MEKRPVILVVMDGIGIREDKKNNAVALANKPTLDKLWAECPHTQ
                     LRAHGLAVGLPTDSDMGNSEVGHNALGCGQIYSQGAKLVNENIESKEIFNSKTWKDLA
                     ENAKGSKMHFIGLLSDGNVHSNISHLKALIKESKNEGIKEVRVHALLDGRDVPATSAL
                     EYVNDIESFMAELNDDNFHACIASGGGRMQITMDRYEADWSMVERGWKIHVMGEGRQF
                     ASTTEAIETYRKELNVVDQDLPGFVIAKDGTPVGTIDDGDSVVLFNFRGDRAQEISLA
                     FDGDASFDKFDRVKVPNVKFAGMLQYDADLQIPKNYLTEPPKIKYTLTEELCKHGIRE
                     YAISETQKYGHVTYFWNGNRSEKFDESLETYVEIPSDVVPFEQRPWMKAAEITDKLCE
                     AIESGNYDFIRTNYPNGDMVGHTGSLPATIIGVESVDLELARVIESVNKVNGILLVTA
                     DHGNADDESKTSHTLAPVPFIVYNAECELKEGKDLGLSNVAATVCDFLGLEPNEHWNP
                     SLKK"
     gene            2623..>3013
                     /locus_tag="ACZPDN_08920"
     CDS             2623..>3013
                     /locus_tag="ACZPDN_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1298782.1"
                     /translation="MERVLIVEDDENIRQLLKLTLASFNYELIDFENGKDAYDYLQDN
                     KVDLAILDLMLPGMNGYDILKFIRSKASLKDLPVIILSAKDKELDKIMGLDLGADDYL
                     TKPFSVEELLARLRVTQRRLLLLQASGD"
     assembly_gap    3014..3023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3024..3303
                     /locus_tag="ACZPDN_08925"
     CDS             <3024..3303
                     /locus_tag="ACZPDN_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1298783.1"
                     /translation="DLDKRIVMIHEEPVELTYKEFELLKYLAKNEGRAVSREELINQI
                     WGYDFIGESRTLDVHINSLRKKLGNEYAHWIQSVRQVGYRLVAGDNDA"
     gene            3416..>4562
                     /locus_tag="ACZPDN_08930"
     CDS             3416..>4562
                     /locus_tag="ACZPDN_08930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1298784.1"
                     /translation="MNTVKLVKNLEIDDLNTTRLGDTFKSEDIRITIIKRNGKVLYDN
                     YKDKISEKHLQREEVKQALEEGTGTCIRHSSTMQRSYLYVADYEDDNQTIVRLAMPFD
                     GLSQSISILLAPFIFSICISFMVVFILSKRMADEIVEPFQDITNTINSTNISEDVLTF
                     NTYNYPELYKITNALTNMSKENKKYLERLEKEKKVRQEFFSNASHELKTPLTSIRGYT
                     ELIRSHTIQDSNQIDTCLDCVLKESDHMTQLINDILTISKLETEEMQVTYSHIQVKKL
                     LDSIIETLRVQIEKMDLKVYVNATEFTVFASMDHIKGIFYNVISNAIKYNKVNGRIDI
                     VLKKNQNNMYFSVEDTGIGIPNKDQNRIFQRFYRVDKQRSKTIPGTGL"
     assembly_gap    4563..4572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4694..>5486)
                     /locus_tag="ACZPDN_08935"
     CDS             complement(4694..>5486)
                     /locus_tag="ACZPDN_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298785.1"
                     /translation="LSLGNKWFRILYWIDLVITLLLIYFPIHLLLSNLKNILPFFILV
                     ACMLVKTMALSQIGGYLKNNRWCKIYFDHTIELDDEDDDWVEKQTKNVDYNKIKEKYA
                     KEMEQDEQDDEDEYETEKAPMTLPQLSIRLGIIIYGELILFPILIQIFSNLFESMDMQ
                     KVFATKDIFVLCIFTAFIWTIPLFYMYYNQKATKKIIACCMIGELIRILVYLPSLMKY
                     YNSHEYSIRVFIFFILIDIIRLLILFTFAIHVLKSEQEYAPNPRD"
BASE COUNT         1933 a          730 c         1054 g         1749 t
ORIGIN      
        1 gttgcaggaa gtaactatcg tgttatggaa acatttttag tttcagctat tttatattta
       61 attttaacaa cacttgcttc attcttattg aattatcttg aaaaaagaat tgatagcgaa
      121 agcagtatga aagatttctg tagctaggag ggtatttatg gaaacaatta ttcaaattca
      181 acatctccag aaacattttg gaaatcttaa agtattaaat gatgtagatt tcgatattca
      241 taaaggagaa gtggttacga ttattggatc gagtggatct ggtaaaagta caatgttaag
      301 atgtgtaaac ttattagaaa gaatggatgg aggccatgtt ctatatcatg gtaaagacat
      361 tctaagtggt caaatggatg tcaatgaatt tcgttctaag gttggaatgg tgtttcaagg
      421 ctttaactta tttaataatt tgactgtttt agaaaactgt atgttaggtc agattcgtgt
      481 tttaaaaaaa tcgaaagaag aagcaaaggc tacagcttta aaattcttag atgtagtagg
      541 aatgaaagaa ttcgcgaata agccaagcac taaactttct ggtggacaaa agcaacgtgt
      601 tgcgattgca cgtgccttgt gtatggatcc agaagtgtta ttgtttgatg agccaactag
      661 tgcattagat ccagaaatgg ttggtgatgt tttggatgtc atgaaaaagt tagcggatga
      721 aggtttgact atggttatcg ttacacacga aatgcagttt gctagagaag taagtgatcg
      781 tgttattttt atggataaag gtgtcattgc agaacagggt agcccagaac aattgttcga
      841 gcatcctcaa caagaaagaa cgcaacaatt tttatctcgt tatcaaagcc gttaagcggc
      901 tttttctttt tgtgaaaaaa ttcatttgtt gagacgagtt cattgaaaaa aggcctagtt
      961 agtgttatat tattatcgat taatggagga aaaatacaat ggaaaaaaga ccagtaatat
     1021 tggttgttat ggacggtatt ggtattcgtg aagacaagaa gaacaatgcg gttgcattag
     1081 ccaacaaacc aacacttgat aaattgtggg ctgaatgtcc acatacacaa cttcgtgctc
     1141 atggacttgc agtaggactt cctacagatt cagatatggg taactcagaa gttggacaca
     1201 acgcattagg ttgtggacaa atttactctc aaggagctaa attagttaat gaaaatattg
     1261 agtctaaaga aatcttcaat tctaaaactt ggaaggattt agctgaaaat gcaaaaggta
     1321 gtaaaatgca tttcatcggt cttttaagtg atggaaacgt gcactctaat atttctcact
     1381 taaaggcttt gatcaaagaa tctaaaaatg aaggtattaa agaagttcgt gttcatgcat
     1441 tacttgatgg acgtgatgtt cctgcaactt cagctttaga atatgtaaat gatattgaat
     1501 catttatggc agaattaaat gacgacaact tccacgcatg cattgctagt ggtggtggac
     1561 gtatgcaaat cacaatggac cgttatgaag ctgactggtc tatggttgaa cgtggatgga
     1621 agattcacgt aatgggtgaa ggaagacaat ttgcttcaac tacagaagct attgaaactt
     1681 atcgtaaaga attaaatgtt gttgaccaag atcttccagg atttgtcatc gcaaaagatg
     1741 gtactccagt tggaactatc gatgatggcg atagcgtagt attattcaac ttccgtggtg
     1801 accgtgctca agaaatctct ttagcatttg atggagatgc ttcatttgat aaatttgatc
     1861 gtgttaaagt tccaaatgtt aaatttgcag gtatgttaca atatgatgca gacttacaaa
     1921 ttccaaagaa ctatttgact gaaccaccaa aaatcaaata cacattgact gaagaattat
     1981 gcaaacatgg tattcgtgaa tatgcaatta gtgaaactca aaaatatgga cacgttactt
     2041 atttctggaa tggtaaccgt tcagaaaaat ttgatgagtc tttagaaact tatgttgaaa
     2101 ttccaagtga cgtagttcca tttgaacaaa gaccttggat gaaggctgct gaaatcactg
     2161 acaaattgtg tgaagcaatc gaaagtggaa actatgattt cattcgtaca aactatccaa
     2221 atggtgacat ggtaggacat actggaagtt tacctgcaac tattattggt gttgaatctg
     2281 tagacttaga acttgcacgt gttattgaat ctgtaaataa agtaaatggt attttattag
     2341 taactgcaga ccatggtaat gcggacgatg aaagtaagac tagccacaca ttagcaccag
     2401 ttccattcat tgtatataat gctgaatgtg aattaaaaga aggcaaagat ttaggattgt
     2461 caaatgttgc agctacagtt tgtgatttct taggattaga accaaacgaa cactggaacc
     2521 catctttaaa aaaataattt agattataag gagtaatgta aaataaactt tactcctttt
     2581 ttatttgcaa tcgtgataaa atgacacaaa gaggttgatg atatggaaag agttttaatt
     2641 gtagaagatg atgaaaatat tcgtcagtta ttaaaattaa cgctggctag ttttaattat
     2701 gagttaattg attttgagaa tggaaaagat gcatatgatt atcttcaaga caataaagtt
     2761 gacttagcta ttctagattt aatgcttcca ggtatgaatg gatatgatat tttaaagttt
     2821 attcgttcaa aagcatcttt aaaagatttg ccagtaatca ttttaagtgc aaaagataaa
     2881 gaattagata aaattatggg attagatcta ggagcggatg actatttgac aaagccattt
     2941 tccgtagaag agcttttggc acggcttcgt gtcacacaaa gaaggctgtt gctcctgcaa
     3001 gcaagtggcg atgnnnnnnn nnntgatttg gataaacgca ttgtaatgat tcatgaagag
     3061 cctgtagaat taacatataa agaatttgaa cttcttaaat atttagctaa aaatgaaggt
     3121 agagccgttt ctcgtgaaga gttgatcaac caaatttggg gatatgattt tatcggtgaa
     3181 agtagaacgt tagatgtaca tattaattca ttacgaaaaa aactaggcaa tgaatatgca
     3241 cattggattc aatctgtaag acaagttggc tatcgtttgg tagctggaga taatgatgca
     3301 taagaaaatt gttcgttcct ttttaattac gtttttaatt acatttcttc ttgggtttgg
     3361 tttactttat gtaagcttgt acaaagcatc catttcacaa gatagacagt atttgatgaa
     3421 tacagtgaag cttgtaaaaa atttagaaat tgatgattta aatacgactc gtttaggaga
     3481 tacatttaaa tcggaagata ttcgtatcac aattattaag cgaaatggta aagttttata
     3541 tgataactac aaagataaga tcagtgaaaa gcatttacaa cgtgaagaag ttaaacaagc
     3601 tctagaagaa ggaacaggta catgtatccg tcattcaagt acgatgcaac gatcctattt
     3661 atatgtggca gattatgaag atgataacca aacaattgtt cgtttagcta tgccttttga
     3721 cggattgagt caatctattt ctattttatt agctccattt atttttagta tatgcatatc
     3781 gtttatggtt gtatttatct tatctaagag aatggccgat gaaattgttg aaccatttca
     3841 agatatcaca aatacaatta attcaacgaa catatccgaa gatgttttaa cttttaacac
     3901 atataattat ccagaacttt ataaaatcac aaatgccctg acaaatatgt caaaagaaaa
     3961 taaaaagtat ttggaaagac tggaaaaaga aaaaaaggtt cgccaggaat tttttagtaa
     4021 tgcgagtcat gaattgaaaa caccattgac ttcgattcgt ggatatacgg aattgattcg
     4081 ttcacatact attcaagata gtaatcaaat tgatacgtgt ttggattgtg tcttaaaaga
     4141 aagtgatcat atgacgcagt tgatcaatga tattttaacc atttctaaat tagaaacgga
     4201 agaaatgcaa gtgacatatt cacatattca agtaaagaag ttattggatt ctattataga
     4261 aactttaaga gtgcaaattg agaaaatgga tttaaaagta tatgtgaatg cgacagaatt
     4321 tacagtcttt gcgagtatgg atcatattaa aggaatcttt tataatgtga tttcgaatgc
     4381 gatcaaatat aataaggtaa atggacgtat tgatattgtg ttgaaaaaga atcaaaacaa
     4441 tatgtatttt agtgtagagg atacgggtat tggaattcca aataaagatc aaaatcgaat
     4501 ttttcaacgt ttttatcgcg tagataaaca acgttccaaa acgatacctg gaacaggact
     4561 tgnnnnnnnn nntatccatt gtaaagcatg ttgtttatta ttataaagga gatattcatt
     4621 taacttcaaa agaagatgta ggaactaaaa taacaattga attacctatt gttgtaaaag
     4681 aaaccccaga gagttagtct ctggggtttg gtgcatattc ttgttcggat tttagaacat
     4741 gaatggcgaa tgtaaataaa atgagtaaac gaatgatatc aattaaaata aagaatataa
     4801 atacacgaat ggaatattca tgtgaattat aatatttcat aaggctaggc aaataaacaa
     4861 gaatgcggat gagttcacca atcatgcaac aagcgataat tttctttgta gctttttggt
     4921 tgtagtacat atagaacaat ggaattgtcc atatgaatgc agtaaatata cagagtacaa
     4981 agatatcttt tgtcgcaaat accttttgca tatccatgct ttcgaataaa tttgaaaaga
     5041 tttgaattaa aattggaaat aaaatcaact ctccataaat aataatacct aaacgaatgg
     5101 ataattgagg taaagtcata ggcgcttttt ccgtttcata ttcatcttca tcgtcttgtt
     5161 catcttgttc catttctttt gcgtattttt ctttgatttt attgtagtct acattttttg
     5221 tttgtttttc aacccaatca tcatcttcat catccaattc aatggtgtga tcaaaataaa
     5281 tcttacacca acgattattt tttaaatatc caccaatttg gctcagtgcc attgttttta
     5341 caagcataca agcgacaagg ataaaaaatg gaagaatatt tttaaggttg gataatagta
     5401 aatggattgg aaaataaatc aataaaagtg tgataactaa atcgatccaa taaagtatgc
     5461 gaaaccattt gttgcctaaa gaaaga
//
LOCUS       DBMDWL010000136         5450 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6033_length_5450_cov_1.586091, whole genome shotgun sequence.
ACCESSION   DBMDWL010000136 DBMDWL010000000
VERSION     DBMDWL010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6033_length_5450_cov_1.586091"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="ACZPDN_08940"
     CDS             <1..876
                     /locus_tag="ACZPDN_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1298786.1"
                     /translation="LFGIVLIHYNRLTVGQLFGIAQASNMVIMPIVNYANLRNMIQSS
                     KPVLQKLLDNSMCYEENEPIVFDEQIHDIKIKHLRYSYGVRQILDLNNLVIDQGKKYL
                     VIGKSGDGKSTFLDILTKQKKADGIYVNDKDLKDVQFSTYADKFSYVNQNNDLLPFSF
                     EQNITLGRKMSKYSLKDLVTIFNLESIFDKERDNLDFEHLNLSGGEKQRICLAKAMYR
                     NKKWLFLDEAFSAIDKTNSDRIHQFILSDPDLTVLSIEHKVTKETVSLYDKVLLFENK
                     KIVSMDVEEYLNSIF"
     gene            1158..1832
                     /locus_tag="ACZPDN_08945"
     CDS             1158..1832
                     /locus_tag="ACZPDN_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1298787.1"
                     /translation="MRIAILEDSLIQQERIRNLLDLSHRLCFFCTVEEYMESNYYFDL
                     LLLDIELGNQNGIEFIKKHPDKQRFIVYVSSHSETMADTFDTNVLGFVTKDRIEEALV
                     EKVNQVEQRIHQLEKIALNLPYETRYVYRDDILYFQIDNGIFVLLKDGTKLSLVNETL
                     KEIEATLSDSFVRVNNNVLINFSKVDYLDVKNHKIIMLNGTIISVSVRRWKKVKEHYI
                     QMRTSI"
     gene            1784..2983
                     /locus_tag="ACZPDN_08950"
     CDS             1784..2983
                     /locus_tag="ACZPDN_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298788.1"
                     /translation="MEKGEGTLYSDEDKHMNILMNSMYYVCEQCFYLKITKNSLYPKW
                     ILSFLLNGLCVFLISEYIHINWLSVFLQLLIPILCLNISTQEHHVLESVISNLIVVLF
                     QTMNILLFRSLTLEYYAFSTVLTICGCAIFYQIFKYESLEFSENMQNGVSIILFIFSY
                     LYMLLMTDLFSKTEFTYMYFIKNSQIYVFMFTISVLVLLSVAIIVFSRIRSKLEIEMY
                     RNFIELSSHYTEQMIHEQTGIKELIHDMRNSLDDIKHLSQNKRYEELESEINRELVKY
                     GKLYPNSICINPYVDVILQNFIQEHKLNYDIQIHVPKRIDSMDTQDLTTLLTCVLDSS
                     LVKENETFMFHMNLKEKELILHVQYPLDIKNMTPSNKSILNIILNKYHGTKIMDSKDM
                     KIILFIE"
     gene            3066..3707
                     /locus_tag="ACZPDN_08955"
     CDS             3066..3707
                     /locus_tag="ACZPDN_08955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298789.1"
                     /translation="MIKIQNLKKVYKDCVALDTVSMHIRKGEFVAIIGASGSGKTTML
                     NIIGGLDTDYMGSCVVRNKEISSLSDVECCTLRSRYISYVYQFFNLISFLTVKENVCL
                     ASQIKGKKVDAKELNQVLKLLGLEHKKDCFANELSGGQQQRVAIARAILAHTDIILAD
                     EPTGNLDGENAENVMDILKVLNEKGKTIVLVTHDLKIANYADRIIRLEQGKIV"
     gene            3704..>5450
                     /locus_tag="ACZPDN_08960"
     CDS             3704..>5450
                     /locus_tag="ACZPDN_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1298790.1"
                     /translation="MNIVKKYRSRNKISYVLLVSILFLCTFLLTSLFVVKDSYDLYRI
                     NAAKSFYGDYDVKYTTHAYTQNKEYTDTYLDSLSYETPLPYAYKGTFDSLVSTTNFSV
                     YPIRLIEGKYPKSNEVLIHKKYQNKYKVGDTIKLYSDQKSKEYTISGVYENLNNQLVN
                     YSFYTATNSKKDAMYVYANLKDKSAIATLPVQDYELNSDMVVAKYHLNVEYENIFKFL
                     ILFMSVCCFLFVYSVLSVHLKKKKAFYDQLYILGMNKKKIRLYMIKECTLLFALIECM
                     GIGFTLLLWGLFLKWIQMNLPFSLHISKYHLCILLLILFVQYIFAFGLSLVRPKHYFF
                     RKLNISKLSIVETMRNGNGLFIALIVAMSSMFICVSMQSLTSWIQTNQQEVQYEHDVS
                     ATFNIFDHTLIPEFIEESKEILKSYEHEFNYTIQCDANINNEQIETKITNEYNDFVLS
                     NSYEDNIRLHLYKQGGELIDVLNLSVEEHINSGDNKVLYIPESKVMEWLNTYPDTFIT
                     GDLWVNTKQSALLKKKLDHIQLSRRDDFYVVDNKTDTKNLAEIMNMCQKIVQFYQFVL
                     MITSIVIIYMQLYQHF"
BASE COUNT         1972 a          662 c          967 g         1849 t
ORIGIN      
        1 ctctttggaa ttgtgttaat acattataat cgattgacag ttggacaatt atttggaatt
       61 gcacaagcta gtaatatggt gatcatgcca atcgttaatt atgcaaatct tcgaaacatg
      121 attcaatctt ctaaaccggt tctacaaaaa ctgttggata attcaatgtg ctatgaagaa
      181 aatgaaccta ttgtttttga tgaacaaatt catgacatta agattaagca tttaaggtat
      241 tcttatggtg ttcgtcaaat attggattta aataatttgg taatagatca gggaaaaaag
      301 tatttagtca ttggaaaaag tggagatggt aagagtacgt ttttagacat tttaacaaag
      361 cagaagaaag cagatggcat ttatgtgaac gataaggatt tgaaggatgt tcaatttagc
      421 acatatgccg ataagttttc ttatgtgaat caaaacaatg atttattacc gttttccttt
      481 gaacaaaata ttactttagg aaggaaaatg agtaaatatt ccttgaaaga tttagtaact
      541 atatttaatt tagaatcaat atttgataag gaaagagaca atcttgattt tgaacatttg
      601 aatttaagcg gtggagagaa gcaacgtatt tgtttggcaa aagcaatgta cagaaataaa
      661 aaatggcttt tcttggatga ggctttttct gcaatcgata aaacaaattc agatcgaata
      721 catcaattca ttttatccga tccagatctt acagtattgt ctatagaaca taaagtaaca
      781 aaagaaacag tttctttata cgataaagtc ttactgtttg aaaacaaaaa gattgtatct
      841 atggatgtgg aagaatattt aaacagtatt ttttgataaa caaaatacac aataaaaact
      901 ccatcgcaaa agactgaatg gatcgggata ccgaatgttt cagaaagacg gagtggatat
      961 tgaaatgtta ccggacggct ggaaacgaac cgggcatgtt atttaggtgt gagacttttt
     1021 cacagaaaaa ttctttctta tttggatgtt tatgtataaa atatgcaagt cacataattt
     1081 tatccgatgt cacaaaccat agataaagtt gaaaggatgc ttttgccatt tactatatta
     1141 cccattataa ttgacctatg agaattgcaa tattagaaga tagtttgatt caacaagaaa
     1201 gaatacgaaa tctattagat ttaagtcata gactttgttt cttttgcaca gttgaagaat
     1261 atatggaatc caattactat tttgatttat tgttgctaga tattgaacta ggaaatcaaa
     1321 atggcattga attcattaaa aaacatccag ataaacaacg ctttatcgta tatgtaagta
     1381 gtcatagtga aactatggcc gatacatttg atacaaacgt cttaggattt gttacgaaag
     1441 atcgaataga agaagcctta gttgaaaagg taaatcaagt agagcaacgt attcatcaat
     1501 tagagaagat tgcactgaat ttaccgtatg agacaaggta tgtgtatagg gatgatattc
     1561 tgtattttca aattgacaat ggcatattcg tcttattgaa agatggaacg aaattatctt
     1621 tagtgaatga aacattaaag gaaatagaag caacgctgtc ggattctttt gttcgagtaa
     1681 ataataatgt gcttattaac ttttctaaag tcgattattt ggatgtaaag aatcataaga
     1741 ttattatgtt aaatggtacg attatttctg tgagtgttcg aagatggaaa aaggtgaagg
     1801 aacactatat tcagatgagg acaagcatat gaatattcta atgaacagta tgtattatgt
     1861 ttgcgaacaa tgtttctatt taaaaataac gaagaattca ctatatccaa aatggattct
     1921 ttcattctta ttaaatggtt tatgtgtgtt tttaatcagt gaatatatac atataaactg
     1981 gctatcggta tttctacaat tgttgattcc cattctatgt ttgaatattt ctacacaaga
     2041 acatcatgta ttagaatctg tcatttcaaa tttgatcgtt gtcttatttc aaacaatgaa
     2101 tatacttcta ttccgttctt tgactttaga atattatgca ttttcaacag tgcttacaat
     2161 ttgtggatgt gcaatctttt atcaaatctt taaatatgaa agtttagaat ttagtgagaa
     2221 tatgcaaaat ggtgtttcta taattctatt tatatttagc tatctttata tgttgttgat
     2281 gacagacttg ttttctaaaa cagaatttac atatatgtat tttataaaga acagccaaat
     2341 ctatgtgttt atgtttacca tatctgttct tgtgttatta agtgtagcta tcattgtttt
     2401 ttctagaata cgctcaaaat tagagattga gatgtatcga aatttcattg aactatccag
     2461 tcattataca gaacaaatga ttcatgaaca gacgggaatc aaggagttga ttcacgatat
     2521 gcgaaattct ttggatgata taaagcattt atctcaaaac aaaagatatg aagaattaga
     2581 aagtgaaata aatagagagt tagtaaaata tggaaagtta tatcccaatt ctatatgcat
     2641 aaatccatat gtggatgtga tcctacaaaa ttttattcaa gaacacaaat tgaattatga
     2701 catacagata catgttccta aaagaatcga ttctatggat acacaagatt taacaacact
     2761 acttacatgc gtattggata gtagcttagt taaggaaaat gagactttca tgtttcatat
     2821 gaatctaaag gagaaagagc taattttaca tgttcaatat cctttggaca taaagaatat
     2881 gacaccttca aataaatcca tactgaatat cattttgaat aagtatcatg gaacaaaaat
     2941 catggattca aaggatatga aaatcatctt atttatcgaa taatcgaaat caattgacgg
     3001 ttattcgatt tttttaatgc ttcttttcct tatgtgtatt aataaagaaa aggagtgggt
     3061 gaattgtgat taaaatacag aatctaaaga aagtgtataa agattgtgtg gcactcgata
     3121 cggtttctat gcacataaga aaaggggaat ttgttgcgat tattggagca agcggaagtg
     3181 gaaaaactac tatgctcaat attattggtg gtttagatac ggattatatg ggaagttgtg
     3241 tagttcgaaa taaagagatt tcttctttat cggatgtaga gtgttgtaca ttaagaagtc
     3301 gatacattag ttatgtatat caatttttta atctgatttc ttttttgaca gtgaaagaaa
     3361 atgtgtgttt ggcaagtcaa attaaaggga aaaaagtgga tgcaaaagaa ttgaatcaag
     3421 tattgaaatt gctagggtta gagcataaaa aagattgttt tgcgaatgaa ttgtctggag
     3481 gtcaacaaca gcgcgttgcg attgctagag ctattcttgc acatacagat atcatattgg
     3541 cagatgagcc aacggggaat ttggatggtg agaatgctga aaatgtgatg gatattttaa
     3601 aagtgttgaa cgaaaaaggg aagacaattg tattggttac gcacgattta aaaatcgcca
     3661 attatgcaga tcgaatcatt cgtttagaac aaggaaaaat tgtatgaata tagtcaaaaa
     3721 atatagaagt cgcaataaga taagttatgt attgttagtg agcattctgt ttttatgtac
     3781 atttctttta acttctctct ttgtcgtgaa agactcttat gatttatatc gaatcaatgc
     3841 agctaaaagc ttttatgggg attatgacgt taaatatacg acgcatgcgt atacacaaaa
     3901 taaagaatat acagatacat acctagatag tttaagttat gaaacaccac tgccttatgc
     3961 gtataaaggt acttttgatt ctttggtttc aaccacaaat tttagtgtct atccaatacg
     4021 tttgatagaa ggaaagtatc ctaaatctaa tgaagtgtta attcataaaa agtatcagaa
     4081 taaatataag gttggagata caataaaatt atattcggat cagaagtcta aagaatatac
     4141 gattagtggt gtgtatgaaa atctaaataa tcagctcgtc aattatagtt tttatacagc
     4201 cacgaattca aaaaaggatg cgatgtatgt ctatgcgaat ttaaaagata aatcggcaat
     4261 cgcaacacta cccgtacagg attatgaatt gaattcggat atggtggttg ccaaatatca
     4321 tttgaatgtt gaatatgaaa atatttttaa atttctaatc ctgtttatga gtgtatgttg
     4381 tttcttgttt gtatatagcg ttttatctgt acacttaaag aaaaagaagg ctttttatga
     4441 tcaactctat atattgggaa tgaataaaaa gaaaattcgt ttgtatatga ttaaggaatg
     4501 taccttgctt tttgcactga ttgagtgtat gggaatcgga tttacattat tattgtgggg
     4561 tttgttttta aaatggattc aaatgaatct tccatttagt ttacatatat caaaatatca
     4621 tctatgtatt ttattattga ttttatttgt acaatatata tttgcatttg gattgagttt
     4681 agtacgacct aaacattatt tctttagaaa attaaatatt tcaaaactat ctatcgttga
     4741 aacaatgaga aatggcaatg gtctgtttat tgcactgatt gttgcgatga gttcaatgtt
     4801 tatatgtgta tccatgcagt cattaacatc ttggattcaa acaaatcaac aagaagtaca
     4861 gtatgaacat gatgtgagtg ccacatttaa tatatttgat catacattga ttcctgaatt
     4921 tattgaggaa tcaaaagaga ttcttaaatc gtatgagcat gaatttaact atactattca
     4981 atgcgatgcg aatataaata atgaacaaat agaaaccaaa atcacaaatg aatataatga
     5041 ttttgtctta agtaattcat atgaagacaa tataagattg catctgtata aacaaggcgg
     5101 agaattaatt gatgtactca atctatctgt agaagaacat ataaatagtg gggataataa
     5161 agttttgtat attcctgaat caaaagtaat ggaatggcta aatacatatc cagatacatt
     5221 tattacgggt gatttgtggg taaatactaa acaatcagca ttactaaaaa agaagcttga
     5281 tcatatccaa ttgtctagaa gagatgattt ctatgtagtc gataataaaa cggacacaaa
     5341 gaatcttgcc gaaatcatga atatgtgtca aaaaatcgta cagttctatc aatttgtatt
     5401 aatgattact tcgatcgtga tcatatatat gcagctgtat caacatttct
//
LOCUS       DBMDWL010000137         5402 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6104_length_5402_cov_2.308055, whole genome shotgun sequence.
ACCESSION   DBMDWL010000137 DBMDWL010000000
VERSION     DBMDWL010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6104_length_5402_cov_2.308055"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="ACZPDN_08965"
     CDS             <1..1219
                     /locus_tag="ACZPDN_08965"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR1298791.1"
                     /translation="VFSKVVDNVCFFFIQHQGYFERDSLYGYQDDGERFGYFQKAVIE
                     MLNQLNYWPNIIHCHDWHTGMIPCMVKETHDADPRYRAIRFVYTIHNMAYQGNFGPEM
                     LDSCLGLPYYLLDNGNVRFDGGISFMKSGILYADKVTTVSPTYAQEILSPQYGEHLEA
                     VLNMRKYDLWGIVNGIDIQLWNPSTDPEIPYHFDKVNIASQKKKAKMALQEELGLEKN
                     PDVMMVGVVSRLTWQKGFYLLMEQLDALCAAPIQLVILGSGESQIENKFRELEDGNKG
                     KICFYYGYNESLAHRIYAASDLFLMPSLFEPCGISQLCSMRYGTLPLVRETGGLKDTV
                     DAYNEYEKSGNGFSFENYDSNELIHTLYYACDVYYNRKEDWEMLQRNALNTDVSWQQS
                     AKTYSLLYDELVD"
     gene            1219..1644
                     /locus_tag="ACZPDN_08970"
     CDS             1219..1644
                     /locus_tag="ACZPDN_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR1298792.1"
                     /translation="MRILVSACLLGYNCKYNGKNNYNSKIVELLKEHEVVPVCPEMLG
                     GLSCPRIPCEKVGDKIMDKEGNDCTKQFIKGAEIALNIAKEKQVDFAILQRRSPSCGY
                     SLIYDGTFNGNLIVGSGIFAQKLQSLDIPIMEAESEKFV"
     gene            1706..1972
                     /locus_tag="ACZPDN_08975"
     CDS             1706..1972
                     /locus_tag="ACZPDN_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HIR1298793.1"
                     /translation="MKQVTVTVVDPVGLHARPATVAVNAASKFKSEINVAFKGREVNM
                     KSIMGVMSLGIPTQSEITISCSGEDEDAAVAAIEEVLRAQKIIA"
     gene            2039..3733
                     /locus_tag="ACZPDN_08980"
     CDS             2039..3733
                     /locus_tag="ACZPDN_08980"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1298794.1"
                     /translation="MSIVEERYSKWLNHENLAPQYKPVLEAMDAEEKNDAFYTTIEFG
                     TAGMRGLLGPGTNRINIHTIRKATQGYANYINENNGGKAGIAIGYDNRHMSKEFAFDC
                     ADLLAKNNIKSYVFESLRPTPELSFAVRHLNCFGGIMITASHNPKEYNGYKLYDDKGC
                     QLIPCLASQVIDKVNAIEDELAIDANCTEEQKKLITVIGKDVDEEYYKNVLSIQLNPE
                     VKKDIKIVFSPEHGTANIPVQEVYKRAGYTCIPVVEQCSPDPDFSNTPTPNPEQAGAY
                     ELSLKYAKENQADLILVCDPDADRMGVGVLHNGEYVVLTGNQSGSVEIEYICSQLQKQ
                     GKMPENPVMFNTVVTSDLGEKVASDYGVSTEKTLTGFKFIGEKVAKYEKTHEKNYVFG
                     YEESYGSLIKPFVRDKDAPQACLMLAEACAFYKEQGKDLVDVLDSLYDRHGTYEESQV
                     ALSLAGEAGAKRIKEILATLRKDAPTQIGGTKVVRSEDYKECTIKEGDTVTELTGFTK
                     SDVLKYYLEDGSWIAVRPSGTEPKCKFYYCIKGASKEDAHNKTVHFQKAMDDLIAK"
     gene            complement(3800..4960)
                     /locus_tag="ACZPDN_08985"
     CDS             complement(3800..4960)
                     /locus_tag="ACZPDN_08985"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1298795.1"
                     /translation="MKCLSTKATSIKPSGIRKFFDLASTMEGVISLGVGEPDFATPWH
                     ICENAIHYMSTGHTHYTANRGLLELREEICKFHKEHYNQVYDPENEVIVTIGGSEAID
                     LCMRALLNPGDEVIVLDPNYVAYAPSVILSDGVVVPLKLKEENDFKILPEDLKKVITP
                     KTKAMIINYPSNPTGGVMTKEDYAKIIDIIKESGIYVISDEIYAELSFDQPFASLAQF
                     DEIKSQIIVVNGFSKAYAMTGWRLGYCMANKELSSVMNKIHQYVIMSAPVMSQYAGIE
                     AIKNGYNDVLMMKEDYLKRRNFLVNRLNRMGLKTNMPHGTFYVFCNIQSTGLDSETFC
                     ERLLQEQKVACVPGNAFGDSGEGYVRISYAYSIDHIKEAVVRLEQFLKDLEN"
     gene            complement(4960..>5402)
                     /locus_tag="ACZPDN_08990"
     CDS             complement(4960..>5402)
                     /locus_tag="ACZPDN_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR1298796.1"
                     /translation="QDLADILNEDEASIKSEIQSLEKNKIICGYHTIINYNKALKDQK
                     VLAYIEVSCTPTRNRGYDKTAALIANYPEVDTMYLLSGDCDFLCLVQGKTMFEVARFV
                     SDKIACVEDVRSTKTLFVLKQYKQSGIVMIDKPEDKESRLVVTP"
BASE COUNT         1816 a          830 c         1029 g         1727 t
ORIGIN      
        1 tgtattctca aaagtggtag ataatgtttg tttcttcttc attcaacatc aaggttactt
       61 tgaaagagat tcattatacg gttatcagga tgatggtgaa cgttttggat atttccaaaa
      121 agctgttatc gaaatgttga atcagttgaa ctattggcct aatattatcc attgccatga
      181 ttggcataca ggtatgattc catgtatggt aaaagaaaca catgatgcag atcctagata
      241 tcgtgctatt cgtttcgtat atactattca caacatggca tatcaaggaa actttggtcc
      301 tgaaatgtta gatagttgtt taggtttacc atactatttg ttagacaatg gtaatgtacg
      361 ttttgatggc ggaatttcat tcatgaaatc tggtatttta tatgcagata aagttacaac
      421 tgtatcacca acttatgctc aagaaatttt atcacctcaa tatggtgaac acttagaagc
      481 tgtattgaat atgcgtaaat atgatttgtg gggaattgta aatggaattg atatccagtt
      541 atggaatccg agtacagatc cagaaattcc ttatcacttt gataaggtga atattgcatc
      601 tcaaaagaaa aaggcaaaaa tggcccttca agaagaatta ggattagaaa aaaatccaga
      661 tgttatgatg gttggagtcg tttcgcgtct aacttggcaa aaaggattct atctattgat
      721 ggaacaatta gatgcattat gtgctgctcc tattcaattg gttattttgg gtagtggaga
      781 aagtcaaatt gaaaataaat tccgcgaatt ggaagatgga aacaaaggca aaatttgttt
      841 ctattacgga tataatgaaa gtttggcaca ccgtatttat gcggcaagtg atctcttctt
      901 gatgcctagt ttatttgaac catgtggtat ttcgcaattg tgttctatgc gttatggtac
      961 tttacctctt gttcgtgaaa caggtggttt aaaagataca gtcgatgcat ataatgaata
     1021 tgaaaaatca ggaaatggat ttagttttga gaactacgat tcaaatgaat tgatccacac
     1081 attgtattat gcttgtgatg tttactataa tcgcaaagaa gattgggaaa tgttacaacg
     1141 caatgcgtta aacacagatg tatcatggca acaaagtgct aaaacatatt ctttgttgta
     1201 tgatgaatta gtagactaat gagaattctt gttagtgcat gtttgttagg atataactgt
     1261 aagtataatg ggaagaacaa ttataattcg aaaattgttg aacttttaaa agaacatgaa
     1321 gtggttcctg tttgtccaga aatgcttgga ggactttctt gtccgcgtat tccttgtgaa
     1381 aaagttgggg ataaaattat ggataaagag gggaatgatt gtacaaaaca gtttatcaaa
     1441 ggagcagaaa ttgctctaaa tatagcgaaa gaaaaacaag tggattttgc gattttacaa
     1501 agaagatcac ctagttgtgg atattcccta atttatgatg ggacttttaa tgggaatctt
     1561 atagttggta gtggtatctt tgcccaaaag ttgcaaagtt tagatattcc aattatggaa
     1621 gcggaatcag aaaaattcgt ttaggtattg ttttctttgg caaaatgtca taaaatacat
     1681 ttagatttga tttaggagga taattatgaa acaagtaact gtaactgtag tagatccagt
     1741 aggtcttcac gcacgtccag caactgtagc tgttaacgct gctagtaaat tcaaaagtga
     1801 aatcaatgta gctttcaaag gacgcgaagt aaacatgaaa tctatcatgg gagttatgtc
     1861 tttaggaatt cctacacagt cagaaatcac aattagctgc tcaggcgaag atgaagatgc
     1921 tgctgtagct gcaatcgaag aagtattgcg cgctcaaaag atcattgctt aattaagaaa
     1981 agtctcatag tttatgagac ttttttattt caaagttaga aaagaaggag aatgaataat
     2041 gtctattgtt gaagaaagat attcaaaatg gttgaaccat gaaaacttag cccctcaata
     2101 taagcctgtt ttagaagcta tggatgctga agaaaaaaat gatgcattct atactacaat
     2161 tgaatttgga actgcaggta tgcgtggtct tttaggtcca ggaacaaatc gtattaacat
     2221 tcatacaatt cgtaaagcaa cacaaggata tgcgaactac attaacgaaa ataatggtgg
     2281 caaagctggt attgcgattg ggtatgataa tcgtcatatg tcaaaagaat ttgcgtttga
     2341 ctgcgctgat ttattagcaa aaaataatat taaatcctat gtatttgaat cattaagacc
     2401 aacaccagaa ttaagttttg cggtacgtca cttaaattgc tttggtggta ttatgatcac
     2461 agcgagtcat aatccgaaag agtataatgg ttataagttg tatgatgata aaggatgtca
     2521 attgattcca tgtttagcaa gtcaggttat tgataaggtt aacgcaattg aagacgaatt
     2581 ggctattgat gcaaattgta cagaagaaca aaagaaattg attacagtga ttggtaaaga
     2641 tgtagatgaa gaatactaca agaatgtatt gagtattcaa ttgaatccag aagtgaaaaa
     2701 ggatatcaag atcgtattct caccagaaca tggtacggca aatatcccag tacaagaagt
     2761 atataaacgt gcaggatata cttgcattcc agttgttgaa caatgtagtc cagatcctga
     2821 tttttcaaac acaccaacac caaacccaga acaagcgggt gcttatgaat tatcattgaa
     2881 atacgcaaaa gaaaatcaag ctgatttgat cttagtatgt gatcctgatg ctgaccgtat
     2941 gggtgttgga gtattgcata atggagaata tgttgtttta actggtaacc aatctggttc
     3001 tgttgaaatt gaatatattt gttcacaact tcaaaagcaa ggtaaaatgc ctgaaaaccc
     3061 tgtaatgttc aatacagttg taactagtga tctaggtgaa aaggtagctt ctgattatgg
     3121 tgtatcaaca gaaaaaacat tgacaggatt taaattcatt ggtgaaaaag tcgctaaata
     3181 tgaaaaaaca cacgaaaaga attatgtatt cggatatgaa gagagttatg gttcattaat
     3241 caaacctttc gttcgtgaca aagatgctcc tcaagcttgt ttgatgcttg cagaagcttg
     3301 tgcattctac aaagaacaag gaaaagattt agtcgatgta ttagatagct tgtatgatcg
     3361 tcatggaaca tatgaagaaa gtcaagtggc attatcatta gctggtgaag caggagctaa
     3421 gcgcataaaa gaaattctag ctacattaag aaaagatgct ccaacccaaa ttggtggtac
     3481 gaaagttgtt cgtagtgaag actataaaga atgcacaatc aaagaaggag atacagtcac
     3541 tgaacttaca ggattcacta aatctgatgt attaaaatac tatctagaag atggaagttg
     3601 gattgcggta cgtcctagtg gtacagaacc aaaatgtaaa ttctattatt gtatcaaggg
     3661 agcttcaaaa gaagatgctc acaacaaaac agttcatttc caaaaggcaa tggatgattt
     3721 gattgctaag taaaattaaa ggatgtcaaa ataaaatggc atcctttttt tgtgtttatt
     3781 tgaaaaaaaa ggatttagac taattttcta aatcctttaa gaattgttct aggcgaacaa
     3841 cagcttcttt aatatggtca atactatacg cataagagat acggacatat ccttctccgc
     3901 tatctccaaa agcgtttcct ggaacacagg ctactttttg ctcttgtaat aatctttcac
     3961 agaatgtttc tgaatcaagc ccagttgatt gaatattaca gaaaacatag aatgtaccat
     4021 gtggcatatt tgtttttaga cccatacgat tcaatcggtt gactaagaaa tttcttcgtt
     4081 ttagataatc ttctttcatc atcaatacat cgttgtatcc atttttgatg gcttcaatac
     4141 cagcatattg ggacataaca ggagctgaca taatcacata ctggtgaatc ttattcatta
     4201 cagaagatag ttctttattg gccatacaat agccaagtcg ccatcctgtc atcgcatagg
     4261 cttttgaaaa accgttaacg acaataattt gagatttgat ttcatcaaat tgagctaatg
     4321 aagcaaatgg ctgatcaaaa ctaagttctg catagatttc atcactaatg acgtaaatgc
     4381 cagattcttt aatgatatca atgatctttg cgtaatcttc tttagtcatg acaccaccag
     4441 taggattgct aggatagtta atgatcatag cttttgtctt tggtgtaatc actttcttta
     4501 aatcttctgg aagaatttta aaatcatttt cttcttttaa ttttaaagga acaacgacac
     4561 catcacttaa tataacacta ggtgcatagg caacataatt tgggtccaat acaatgactt
     4621 catctccagg atttaaaagg gcacgcatac ataggtcaat ggcttcactt ccaccaattg
     4681 taacaatgac ttcattttca ggatcgtata cttgattgta gtgttcttta tgaaatttac
     4741 agatttcttc acgaagttct aataaaccac ggttagcagt atagtgtgtg tgacctgtgc
     4801 tcatataatg aattgcattt tcacagatgt gccatggcgt cgcaaaatct ggctcaccaa
     4861 cacctaaaga gataacccct tccatggtgc ttgctaaatc aaaaaatttt cggattccac
     4921 tcggtttaat ggatgttgct tttgtagata aacatttcat tatggagtca caaccaatct
     4981 tgattctttg tcttcaggct tgtcaatcat aacaatacca ctttgtttat attgctttaa
     5041 gacaaataaa gtcttggtag atcgtacatc ttctacacaa gcaattttat ccgatacaaa
     5101 gcgagccact tcaaacattg tttttccttg cactaaacac aagaaatcac aatctccact
     5161 taaaagatac atagtatcga cttctggata attggcaatc aatgctgccg ttttgtcata
     5221 ccctctattt ctagttggtg tacaacttac ttctatataa gctaagactt tttggtcttt
     5281 taatgctttg ttgtagttga taatcgtgtg ataaccacaa atgattttat ttttttctaa
     5341 agattgaatc tcggatttta tactcgcttc atcttcgttt agaatatctg ctaaatcttg
     5401 tg
//
LOCUS       DBMDWL010000138         5258 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6351_length_5258_cov_1.833818, whole genome shotgun sequence.
ACCESSION   DBMDWL010000138 DBMDWL010000000
VERSION     DBMDWL010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6351_length_5258_cov_1.833818"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(111..1724)
                     /locus_tag="ACZPDN_08995"
     CDS             complement(111..1724)
                     /locus_tag="ACZPDN_08995"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1298797.1"
                     /translation="MSTKYIFVTGGVVSGLGKGISAASLGRLLKSRGYKVTSQKLDPY
                     INVDPGTMSPFQHGEVYVTDDGLEADLDLGHYERFIDENLHTYSNLTTGRVYWNVINK
                     ERDGKYLGETVQVIPHITNEIKSFVIRAGKHAAADVVITEIGGTTGDIESLPFLESIR
                     QVALEVGRKNCLFIHVTYIPYISGSQEYKSKPTQHSVKELQSYGINPDIIVARCDETI
                     PQSVLDKISLFCNVEKRCVIQNKTLPSLYEVPFMLEEQNLADIVCEKTGLEIRKPDLT
                     EWKQVVENIKASTKPVNIALVGKYVALHDAYLSVSEALYHGGFENHAKVNIQWIDSEG
                     LENANLDEVFKDTDGIILPGGFGDRGIEGMISVANYARNKQIPYLGICLGMQIAAIAF
                     ARDVLGFEDANSYEFDSESKHTIIDFMEDQSNDIKKGGTMRLGSYPCSLKADTKIWSC
                     YKKDMIDERHRHRYEFNNEYRSEFERNGMNIVGTSPDKQLVEAIEYTNHPFYIGVQYH
                     PEFKSRPNRPHPLFVGLVKASLENKESHK"
     gene            1858..3015
                     /locus_tag="ACZPDN_09000"
     CDS             1858..3015
                     /locus_tag="ACZPDN_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1298798.1"
                     /translation="MDLHEKEKMYQRLIIFTLLIGMVIYYFEKIFCFLGWIGQMCLPF
                     LLGAGLAFVFNIISNNLMRYSIKWFDIKDTKIKRIISNVLSVLIVFAVFVAFVGIVIP
                     RILFSIQLLLTNFPKMIYELYIWLLDVTKNIDVIHDSLKNLDINAFSTFAIDNVSKKI
                     SSWLVSGGANDIFGSIFTILSTTFSWFAQAFITIVFSFLLLFNKEQVVHETKSLLKAY
                     LPEKNYDRVRHVLKLIVRIFTSYVGGTCLECLILASLVTVGSLILKIPYAFLVGLVVG
                     IGALVPMFGALVAAILCAFFIAMTSPVQGLTFIIMFICIQQVEGNFIYPHVVGKSVGF
                     PPMYIIVAITIGANVAGIIGIIIFIPICSCIYQLVSEDVLSRLQKVKEVTD"
     gene            3076..3414
                     /locus_tag="ACZPDN_09005"
     CDS             3076..3414
                     /locus_tag="ACZPDN_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298799.1"
                     /translation="MNAEKRLAMDLLDAVWKEDVQKVEYCLQLGASPSWVFNGYPILM
                     HAISIGNEEIVNILIDAGAIQKQEALGFALERGIGQMIRPLVFRGIVPKHFEPKKGFG
                     YYPQRFCSTH"
     gene            3427..4377
                     /locus_tag="ACZPDN_09010"
     CDS             3427..4377
                     /locus_tag="ACZPDN_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024676.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1298800.1"
                     /translation="MNRTNLCIRMLQLLKARGKMNTTQLASELEVNPRNIREFKKELV
                     LAGYNVEEVKGRYGGYILNDQYDLPVTIFSDEQKDALVQSYKFMRTQKDFKPLSLYCD
                     AMDKIIATSKISSNDSLHYMSNSIKLDQKTSTMIDKTQQAINLGLCVLLSYQSLQDET
                     PKEFEVEPYEIIHYHKSYYLIGFSLLRKDYRMYRFSLERMYSCEISSRHFIRDNHFHL
                     SNYIGEHSLIPLDLKQIQVRVYHNENGLRLFKEKYWGNKLQIINKQEGFTDFEFYTDD
                     MSNLYQSLFAMTNQVEILQPEDVKSNYVMRVKKILNAYKG"
     gene            4422..>5258
                     /locus_tag="ACZPDN_09015"
     CDS             4422..>5258
                     /locus_tag="ACZPDN_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HIR1298801.1"
                     /translation="MLHVSDLMREMKCDCFCWNSVHNSIHQESFYKMDCPFSNLWQTY
                     LNATNCGLGHSMDSNEQSLKLLEENDVVCFARFEYKECRTKIPYLKKVENGYMAVYPH
                     LSAYPKENEALIMKINEIILSHCGIHVTQNRIVYLNKEYIRKESLDLNELLCISDKLF
                     NKRNHLSKTIAECMDEVDIDLDRWIERTKEILNRTSITPVRTKQCTALRRCNYYSVCF
                     DESNEPDDSILFFTTNQHKLDAYNRGIRHIHELPLNQIEGFRLQYAQYMASKTNKPFM
                     DRA"
BASE COUNT         1737 a          860 c          908 g         1753 t
ORIGIN      
        1 tggtttatga tcgttcgttt ttctagtaca atggatataa agtctgtagc ttccatatta
       61 aatatgtctg tggatgaact tgaaaagtat atataaaaaa aggaaagtca ctatttgtga
      121 ctttctttgt tttctagact tgcctttact aaaccaacaa acaatgggtg agggcgattt
      181 ggtctagatt taaattcagg atgatattga acaccaatat agaatggatg atttgtatat
      241 tcaatagctt ctactaattg tttatctgga cttgtaccaa cgatgttcat accattacgt
      301 tcgaattcac tgcgatattc attattgaat tcataacgat gacgatgtct ttcatcgatc
      361 atatcttttt tgtagcaaga ccagattttt gtatccgctt ttaaagaaca aggataagaa
      421 cccaaacgca tagtgccacc ttttttgatg tcattgcttt gatcttccat aaaatcaatg
      481 attgtatgtt tactttcaga atcaaattca tacgagttgg catcctcaaa acctaataca
      541 tcacgcgcaa aggcaatggc tgcaatttgc atacccaaac agattcctaa ataagggatt
      601 tgtttatttc ttgcatagtt ggcaacactg atcatacctt caataccacg atctccaaaa
      661 ccaccaggta aaataattcc atctgtatct ttaaatactt catctaaatt ggcattttct
      721 aaaccttcag aatcaatcca ttgaatattc actttggcgt gattttcaaa accaccatga
      781 tataaagctt cacttacaga aagatatgca tcatgtagtg ctacatattt accaaccaag
      841 gcaatattta caggttttgt acttgcttta atgttttcaa caacttgttt ccattctgtt
      901 aaatctggtt tacgaatttc tagacccgtt ttttcacaga caatgtccgc aagattttgt
      961 tcttccaaca taaatggaac ttcataaagg gaaggaagtg tcttattttg gatcacacaa
     1021 cgcttttcaa cgttacagaa taatgaaatt ttgtctaaaa cagactgagg aatggtttca
     1081 tcacaacgcg caacaataat atcaggatta ataccataag actgtaattc tttgacagaa
     1141 tgctgagttg gttttgattt atattcttga gaaccagaaa tatatgggat atacgttaca
     1201 tggatgaata aacaattttt acgtcctact tctaaggcca cctgacgaat ggattctaaa
     1261 aaaggtaagc tttcaatatc acccgttgta ccaccaattt cagtgatcac aacatctgcc
     1321 gctgcatgct taccagcacg aatcacaaaa cttttgattt cattggtaat atgagggatg
     1381 acttgaacgg tttcaccaag atattttcca tcacgctctt tgttgatgac attccaatag
     1441 acacgtccag tggtaaggtt ggaatacgtg tgtaggtttt catcgataaa tcgttcataa
     1501 tgacctaaat ctagatcggc ttcaagacca tcatctgtga cataaacttc accatgttgg
     1561 aaaggggaca ttgttcctgg atccacattg atataaggat ctaatttttg gcttgtaacc
     1621 ttgtagccgc gagactttaa aagacggcca agacttgctg cactgattcc tttacctaaa
     1681 ccagaaacaa caccacccgt tacgaaaatg tactttgtac tcatttgttt cacctctact
     1741 ttttttcttt taactcggca attttacacc tatgaaaaaa agatgtaaat aaaaaattta
     1801 aaaaaatgat tttcatttta aaagatagta taataatttc gatttgaggt ggttttagtg
     1861 gacctacatg aaaaagagaa gatgtaccaa agacttataa tttttacact attgattgga
     1921 atggttatct attattttga aaagatcttt tgttttttag gttggattgg acaaatgtgc
     1981 ttaccatttt tacttggagc cggtttggct tttgtgttta atattatttc gaataatcta
     2041 atgcgctatt cgataaaatg gtttgacatc aaagatacaa aaatcaaacg aattatttcg
     2101 aatgtattat cggttctaat cgtttttgcc gtctttgtcg catttgttgg aattgtcata
     2161 ccaagaattt tattttctat tcaattgctg ctgacaaatt ttccaaaaat gatttatgaa
     2221 ttatatattt ggcttttaga tgtcacaaaa aatattgatg taattcatga ttccttaaaa
     2281 aacttagaca tcaacgcctt tagtacattt gcgattgata atgtctcaaa aaaaataagt
     2341 tcatggcttg tttctggggg agccaacgat atattcggct caatttttac aattttatcc
     2401 acaacatttt catggtttgc gcaagccttt atcaccattg tcttttcttt tttattgctc
     2461 tttaataaag aacaagtcgt acatgaaaca aaatctttat taaaagccta tcttcctgaa
     2521 aaaaactatg atcgagtgcg ccacgtatta aaacttattg ttcgcatatt tacaagttat
     2581 gtaggaggaa cctgcttaga atgtttaatt cttgcaagtt tagtcactgt aggatccttg
     2641 attttaaaaa taccatacgc atttttagtt ggacttgtcg tgggtattgg cgctttggta
     2701 cctatgtttg gagctttggt cgccgccata ttatgcgctt tctttattgc gatgacatcc
     2761 cctgtacaag gtctaacttt tatcattatg tttatttgta tccaacaagt cgaaggaaac
     2821 tttatttatc ctcatgtcgt tggaaaatcc gttggctttc cacccatgta tattattgtt
     2881 gcgatcacaa ttggagcaaa tgttgcaggt attattggaa tcattatctt tattccaatt
     2941 tgttcgtgta tttatcaatt ggtgagtgaa gatgtgttaa gtcgtcttca aaaagtaaaa
     3001 gaggtaaccg attaatttga cataaatgat gtctatttaa attgtatcct ttgagtatag
     3061 aaagaaggat acaatatgaa cgcagaaaaa agattagcta tggatttatt agatgccgta
     3121 tggaaagagg atgtacaaaa agttgaatat tgtttacaac ttggtgcaag tccaagctgg
     3181 gtatttaatg ggtatccaat tttgatgcat gccatcagca ttggaaatga agaaattgta
     3241 aatattttaa ttgatgccgg agccattcaa aaacaagagg ctttaggttt tgcgcttgaa
     3301 agagggattg gtcaaatgat acgtccactt gtatttcgtg gtattgttcc taaacatttt
     3361 gagccaaaaa aaggctttgg ttactatccc caaagatttt gcagcacaca ctaaaaggag
     3421 aaaacaatga atcgaacaaa cttatgcatt cgcatgttac aacttttaaa ggcaagagga
     3481 aagatgaata caacacaact tgcttcagaa ctagaagtaa atccacgaaa tattcgtgaa
     3541 tttaaaaaag agcttgtgct tgcaggctat aacgtagaag aagtgaaagg acgctatgga
     3601 ggctatatct taaatgatca atatgatctt ccggtcacta tttttagtga cgaacaaaag
     3661 gatgcattgg tacaatcgta taaatttatg cgtacacaaa aagattttaa accactctct
     3721 ttatattgtg atgccatgga taagattatt gcgacaagta aaatttcatc aaacgattca
     3781 ttacactaca tgtcaaattc aatcaagttg gatcaaaaga cttcaactat gatcgacaag
     3841 acgcaacaag ccataaatct aggtctttgc gtccttttgt cgtaccaatc tttacaagat
     3901 gaaaccccca aagaatttga ggttgagccc tacgaaataa ttcactatca taaatcctat
     3961 tatttaattg gcttttcctt attgagaaaa gattatcgta tgtatcgttt cagcttagaa
     4021 cgtatgtatt catgtgaaat ctcatcgaga cattttattc gtgataatca tttccattta
     4081 agtaactata ttggagagca ttccctaatt cctttagatt taaaacaaat acaagtcaga
     4141 gtctatcata acgagaatgg tcttcgtttg tttaaagaaa aatattgggg taataaacta
     4201 caaatcatca ataaacaaga gggtttcacc gactttgaat tttatacaga cgacatgtcg
     4261 aatttgtatc aatctttatt tgcgatgaca aatcaagtag aaatcttgca gccagaagat
     4321 gtgaagtcaa actatgttat gcgcgttaaa aaaatattaa acgcttataa aggatgaaaa
     4381 gatttgattt ttgaagtaca ataagaatgg tgaaaaatat tatgttacat gtatcagatt
     4441 taatgcgtga aatgaaatgt gactgttttt gttggaacag tgtccacaat tcaattcacc
     4501 aagaaagttt ttataaaatg gattgtccat tctcaaacct ttggcaaacg tatctaaacg
     4561 caacaaattg cggtttaggt cactccatgg attcaaatga acaatcttta aagttattag
     4621 aagaaaatga tgtcgtatgt tttgcacgat tcgaatataa ggagtgtcgt acaaaaattc
     4681 catatctaaa aaaagtagag aatggatata tggctgtata tccacattta agtgcatatc
     4741 caaaagagaa tgaggcgttg attatgaaaa tcaatgaaat cattctatct cattgtggta
     4801 ttcacgtgac tcaaaatcgc attgtgtatt tgaataagga atatattcga aaagaaagtc
     4861 tagatttaaa tgaattgtta tgcataagtg ataagctatt caataagcgt aatcatttat
     4921 cgaaaacgat agccgagtgt atggatgaag tggatattga tttggatcgt tggattgaaa
     4981 gaactaaaga aattttgaat cgtacgagca tcactccagt gcgtacaaaa caatgtacgg
     5041 cattaagaag atgtaattat tattccgtat gttttgacga atccaatgaa cccgatgatt
     5101 ctattttgtt ttttacgacc aatcaacata aattagatgc ctataatcgt ggtattcgtc
     5161 atattcatga attgccattg aatcaaattg aagggtttcg tcttcaatat gcgcaatata
     5221 tggcaagtaa aacgaataaa ccatttatgg atcgggcc
//
LOCUS       DBMDWL010000139         5250 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6370_length_5250_cov_2.903671, whole genome shotgun sequence.
ACCESSION   DBMDWL010000139 DBMDWL010000000
VERSION     DBMDWL010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6370_length_5250_cov_2.903671"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="ACZPDN_09020"
     CDS             complement(<1..400)
                     /locus_tag="ACZPDN_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298802.1"
                     /translation="MSLLAAIILISLILCAIAIPFFIRSFLWQSILKKIYAGQYDKAI
                     QQMNAPLYTFLFSEYEKNYNILRTYISKQDTNEITKQVEFMLSQNLNNSQAYQVASMT
                     YFYFLDVQNVELSKKLLDIIERKGNEDEIAY"
     gene            679..1125
                     /locus_tag="ACZPDN_09025"
     CDS             679..1125
                     /locus_tag="ACZPDN_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1298803.1"
                     /translation="MIKDLLKEKNCFIKESVKDWKDAICVACAPMIEQGYCTKEYEDA
                     VFKNTEKFGPYYVLCENLALIHASNQSGVNETQMAVTVLKEPVKFKPDGYDVRILVTL
                     VAKDNHSHMEGIQAVSNIFADELKVNSLLNASTPQEIYNIFVENVG"
     gene            1153..2574
                     /locus_tag="ACZPDN_09030"
     CDS             1153..2574
                     /locus_tag="ACZPDN_09030"
                     /inference="COORDINATES: protein motif:HMM:NF006922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HIR1298804.1"
                     /translation="MLNFIVNILSTPAILVGLLSLIGLALQKKPIEEIVKGTVKTIVG
                     FLVLSAGASFLQTGSLNAFGDIFNYAFNMQGVVPNNEAIVSLALKDFATDTAYIMCLG
                     MIANIILARFSRWHYIFLTGHHTLYMACMLAVILNVGGLSGAQLWLAGGLVLGFIMVV
                     SPAYCEPTMRKIVQTDELGFGHFGGAGYWFSAQCAKLFKGKSKSTEEVNFPQRLTFLR
                     DTTVAIGLTMVVFFLIVTGVAVGRGILAANPETILATYPNLGGLLNVGAETQTHWAVW
                     AITSGLSFAGGVYIILSGVRLIVGEIVPAFRGIAEKLVPGAIPAIDCPVVFPYAPNAV
                     LIGFLVSFLGGIVGLFVLAFIDKSLVPCALILPGVIPHFFCGATAGVFGNAEGGLKGC
                     VFGSFMHGLLITFLPAICMPVMGSLNFANCTFSDADFSWLGIVLGNIAQHVQGTGLLV
                     ICVVIFLLPIVYNFVAPKKAEEK"
     gene            2589..2867
                     /locus_tag="ACZPDN_09035"
     CDS             2589..2867
                     /locus_tag="ACZPDN_09035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR1298805.1"
                     /translation="MAIRSIMCCCGQGLGSSMMVSMNVEKALKNLGITGVSVDHTALS
                     EANPNSADLFVVGRDLAPQVASYPNKIILDRIMDMNELTEKLKEVFGK"
     gene            2917..3777
                     /locus_tag="ACZPDN_09040"
     CDS             2917..3777
                     /locus_tag="ACZPDN_09040"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1298806.1"
                     /translation="MEEIMDKKVEILAKKVRYDILDMLQHRGYGHLGGSLSLVELFAV
                     LYGKQMKYDPKNPKWEGRDRVVLSKGHAGPVWYSTLSECGFFDKEKLMTLNDGGTMLP
                     SHPDRTKTPGVDMTTGSLGQGTSTAAGIATALKMKHSDNYVYLIVGDGELNEGQCWEA
                     FQYLANYKMNNCIVIIDDNKRQLDGYTKDVINPFNIEAKMKAFGFNTQTVKGNDLDAI
                     DEAITKAKSVTDSAVCIVLDTIKGQGVKFFEELLDNHSVKFNTEEVNRATDEAKASLK
                     KEIEEEEQHV"
     gene            3770..4726
                     /locus_tag="ACZPDN_09045"
     CDS             3770..4726
                     /locus_tag="ACZPDN_09045"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1298807.1"
                     /translation="MYKLVENRSEKGKELRVAVVESIQELIKENDKVVAMEADLGGAS
                     KFTNIKKSNPENFIQCGISEANMIGVGCGLSSEGFIPYVHTFGPFATRRVYDQIYLSG
                     AYAHNTINIYGSDPGFTVGPNGGTHTTWEDVALMREIPGSVIVDPCDETQTRWVVKEL
                     SKHEGVHYIRANRKDVRNVYTPDSTFELGKGNVLREGKDILIIVSGQLVSDALDCAEK
                     LVEEGYSVEVIDMYCIKPLDIDLVLSESKGKKAVVTFENHSIYGGLGSAVAEVLMENG
                     VNVPFKRHGVDERFGQVGTPDFLQKEFQLTADDLYRTIKSML"
     gene            complement(4814..5002)
                     /locus_tag="ACZPDN_09050"
     CDS             complement(4814..5002)
                     /locus_tag="ACZPDN_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298808.1"
                     /translation="MKVLKEESANRGIQMGIKQDIEKGIEHGVKTIQIKIAIKTLEEI
                     SEIVGLDLNTLREPKKCI"
     gene            complement(5008..>5250)
                     /locus_tag="ACZPDN_09055"
     CDS             complement(5008..>5250)
                     /locus_tag="ACZPDN_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298809.1"
                     /translation="EEIEMCEAEKRWLEVKSKEWEAEGIRKGIEQGIEQGIEQGSKNN
                     RKEMYQTMMDKGFSISSIASIFSVSEDSIKKLLMKA"
BASE COUNT         1691 a          773 c         1013 g         1773 t
ORIGIN      
        1 catacgcaat ttcatcttca ttaccttttc gctcaatgat atctaataat tttttagata
       61 actctacatt ttgaacatcc aaaaagtaaa agtaggtcat actggcaact tggtatgctt
      121 gtgaattgtt taaattttgc gaaagcatga attcgacttg ttttgtaatt tcatttgtat
      181 cttgtttcga aatatatgta cgtaaaatat tatagttttt ttcgtattca gaaaataaga
      241 atgtatatag tggagcattc atttgttgaa tagccttgtc atattgtcca gcatagattt
      301 tttttagaat agattgccat aagaagcttc ggataaaaaa tggaatggcg atagcacata
      361 aaataagcga aattaatata attgcagcta atagactcat agattacaga aacccctttc
      421 ataaccatct ttaaaataac aattgttaaa gattttatca aatccaattt ttttcacgat
      481 aagcagccta gtttgtgcaa gaaaaagtat tttaagcata ttttgcacaa aaaatataca
      541 cgtatataat gataatagtg taaaacgctt acagacaagc acaaaatgta cattttgcac
      601 aatgtttgtg caaaacattg ttcttgtttt tgaaaaccat ttcacagata atgcaggtgt
      661 gaagaggaga gatacgagat gattaaagat ttgctaaaag aaaaaaattg ttttattaaa
      721 gaaagtgtaa aagattggaa agatgcaatt tgtgttgctt gtgcaccaat gattgaacaa
      781 gggtattgta caaaagaata tgaagatgct gttttcaaaa acacagaaaa atttgggccc
      841 tattatgtgt tatgtgaaaa cttggctctg atccatgcga gcaatcagtc tggtgttaat
      901 gaaacacaga tggcggttac ggttttaaaa gaacctgtaa aattcaagcc tgacggatac
      961 gatgtgcgaa ttttagttac acttgtcgca aaagataatc attctcacat ggaaggcatt
     1021 caagctgttt ctaatatctt cgcagatgaa ttaaaagtaa attcgttact aaatgcttca
     1081 actccacaag agatatataa tatctttgtt gaaaatgtgg gataagctca ggtaacatag
     1141 aaggagaaag atatgttaaa ttttatcgtt aatatcttgt ctacacctgc cattttagtt
     1201 ggtttattat ctttaattgg tttagcatta cagaaaaaac caatcgaaga aatcgtaaaa
     1261 ggtacagtta agacaattgt tggtttctta gtattaagtg ctggtgcatc gttcttacaa
     1321 actggatcat taaatgcatt cggtgatatc ttcaactatg cattcaatat gcaaggtgtt
     1381 gttcctaaca atgaagctat cgtttcatta gcattgaaag atttcgctac tgacacagct
     1441 tacattatgt gtttaggtat gatcgcaaat attattttgg cacgtttctc aagatggcac
     1501 tacatcttct tgactggtca ccacacatta tacatggctt gtatgttagc tgttattcta
     1561 aatgttggtg gattatcagg agctcaatta tggcttgctg gtggattggt gttaggattc
     1621 atcatggttg tcagtcctgc ttattgtgag cctacaatgc gtaaaattgt tcaaacagat
     1681 gaattaggat ttggtcactt tggtggtgct ggatactggt tctctgctca atgtgctaaa
     1741 ctattcaaag gaaagagtaa atcaactgaa gaagttaact tccctcaacg tttaactttc
     1801 ttacgtgata ctacagttgc tattggttta acaatggtag tattcttctt gatcgtaact
     1861 ggtgttgctg ttggacgtgg aatcttagct gcaaatcctg aaactatttt agctacttat
     1921 ccaaacttag gtggattatt aaatgttggt gcagaaacac aaacacactg ggctgtttgg
     1981 gctattactt ctggcttaag ctttgctggt ggtgtatata tcattttaag tggtgtacgt
     2041 ttgatcgttg gagaaatcgt tcctgcattc cgtggtattg ctgaaaaatt ggttccaggt
     2101 gctattcctg caatcgactg tccagttgta ttcccttatg ctccaaatgc tgtattgatt
     2161 ggtttcttag tatcattctt aggtggtatt gttggattgt tcgtattagc atttattgac
     2221 aaatcattag ttccatgtgc attgatttta cctggtgtaa ttcctcactt cttctgtggt
     2281 gctactgcag gtgtattcgg taatgctgaa ggtggattaa aaggttgtgt atttggaagt
     2341 ttcatgcatg gtttattgat tacattctta ccagcaatct gtatgccagt tatgggttca
     2401 ttgaactttg ctaactgtac attctcagat gctgacttct cttggttagg tattgtatta
     2461 ggtaatattg ctcaacatgt tcaaggaact ggattattag taatctgcgt tgtaatcttc
     2521 ttattaccaa tcgtttataa ttttgtagct cctaaaaaag ctgaagaaaa atagaaaggg
     2581 gaatgaaaat ggctattcgt agtattatgt gctgctgtgg tcaaggactt ggaagtagta
     2641 tgatggtaag tatgaatgtt gaaaaagcat tgaaaaacct aggtattact ggagtttctg
     2701 ttgaccatac agctttaagt gaagcaaatc ctaactctgc agatttattt gtagtaggac
     2761 gtgacttagc tccacaagta gcatcatatc ctaacaaaat cattcttgat cgtatcatgg
     2821 atatgaatga attaactgaa aaacttaaag aagttttcgg taagtaaaat ttgataaaaa
     2881 gaagggtttt atatctcctt ctttttgttt agccatttgg aggaaattat ggataaaaaa
     2941 gtagaaatat tagcaaaaaa agtgcgttat gacattttgg atatgttaca acatagaggt
     3001 tatggacact taggtggatc tttatctttg gttgaattat ttgcggtatt gtatgggaaa
     3061 caaatgaaat atgatcctaa aaatccaaaa tgggaaggaa gagatcgcgt tgtattgtca
     3121 aaaggacatg caggaccagt ttggtattca acattaagtg aatgtggttt ctttgataaa
     3181 gaaaagttaa tgacattaaa tgatggtgga acaatgttgc caagccatcc agaccgaaca
     3241 aaaacacctg gagtcgatat gacaacagga tcattaggtc aaggaacaag tacagctgca
     3301 ggaattgcca cagcattaaa gatgaagcac agtgataact atgtatatct tattgttgga
     3361 gatggtgaat taaatgaagg tcaatgttgg gaagccttcc aatacttggc aaactacaaa
     3421 atgaataatt gtatcgttat cattgatgac aacaaacgtc aattagatgg atatacaaaa
     3481 gatgttataa atccatttaa cattgaagct aagatgaagg catttggttt taatacacaa
     3541 actgtcaaag gaaatgattt agatgcgatt gatgaagcta tcacaaaagc taaatcagtg
     3601 actgattcag ctgtatgtat tgttttggat acaatcaaag gtcaaggtgt taagttcttt
     3661 gaagaacttt tggataatca ctcagtaaaa ttcaatacag aagaagtgaa tcgagcaact
     3721 gatgaggcta aagcttcttt aaaaaaggaa attgaagagg aggagcaaca tgtataaatt
     3781 agtagaaaat cgtagtgaaa aaggtaaaga attacgtgtt gcagttgttg aaagcattca
     3841 agaacttatt aaagaaaatg ataaagtggt tgctatggaa gccgatttgg gtggagctag
     3901 taagttcaca aatatcaaaa aatcaaatcc agaaaatttc attcaatgtg gtatttcaga
     3961 agcaaatatg attggcgttg gatgtggatt atcaagtgaa ggatttattc cttatgtgca
     4021 tacatttgga cctttcgcaa cacgtcgagt ttatgatcaa atttatttat caggtgcata
     4081 tgcgcataat acaatcaata tttatggttc agatccagga tttacagtag gacctaatgg
     4141 aggtacacat acaacttggg aagatgtggc tttgatgcgt gaaattccag gttcagtcat
     4201 tgtggatcct tgtgatgaaa cacaaacaag atgggttgta aaagaacttt ctaaacatga
     4261 aggtgtacat tatattcgtg caaaccgtaa agatgttaga aatgtgtata caccagattc
     4321 aacttttgaa ttaggcaaag gaaatgtttt aagagaaggt aaagatattc taattattgt
     4381 atctggccag ttggtaagcg atgcattaga ttgtgctgaa aaattagttg aagaaggata
     4441 ttcagtagaa gtaatcgata tgtattgtat taaaccttta gacattgacc tagtgttatc
     4501 tgaatcaaaa ggtaaaaagg ctgttgttac gtttgaaaat cattcgattt atggtggact
     4561 tggaagtgct gttgcagaag ttttgatgga aaatggagta aatgttccat ttaaacgtca
     4621 tggtgttgat gaacgttttg gacaagtagg aacgccagat ttccttcaaa aagaattcca
     4681 actaactgca gatgatttat atagaacaat taaatcaatg ctttaattgg atgataaaat
     4741 aatgtaaaaa aggaaattca cattttaata tgaatttcct ttttcaattc cattatcacc
     4801 gaccgcattt caatcaaata cacttttttg gttctcttaa tgtatttaaa tctaaaccaa
     4861 caatttcaga gatttcttca agcgttttaa tcgctatttt gatttgtatg gtttttacac
     4921 catgttcaat gcctttttca atatcttgct tgatacccat ctggattcct ctgtttgccg
     4981 attcttcttt caacactttc actctaatta agctttcatc aatagctttt taattgaatc
     5041 ttcacttacc gagaagattg atgcgatact tgaaatactg aatcctttat ccatcatcgt
     5101 ttgatacatt tcttttcggt tattcttact gccttgttca ataccttgct caattccttg
     5161 ttcgattcct tttcttattc cttcagcttc ccattctttt gactttactt caagccatct
     5221 cttttcagct tcacacattt caatctcctc
//
LOCUS       DBMDWL010000140         5227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6429_length_5227_cov_3.238002, whole genome shotgun sequence.
ACCESSION   DBMDWL010000140 DBMDWL010000000
VERSION     DBMDWL010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6429_length_5227_cov_3.238002"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            95..418
                     /locus_tag="ACZPDN_09060"
     CDS             95..418
                     /locus_tag="ACZPDN_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR1298810.1"
                     /translation="MKLILAILSNDDSPVASNALNKNGFSVTKLATTGGFLRAGNTTL
                     IIGCEDEKVEAAIDIIKEYSSKRTEIVPSTASYDIGRYASFPVEVTVGGATIFVLDVA
                     QFLKV"
     gene            539..1771
                     /gene="purD"
                     /locus_tag="ACZPDN_09065"
     CDS             539..1771
                     /gene="purD"
                     /locus_tag="ACZPDN_09065"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1298811.1"
                     /translation="MKILVIGKGGREHALAYACKRSALCDELICAPGNPGMAKIGECV
                     DVKDNDVDGLVALAKERNVDLTIVGPEATLALGVVDAFKKEGLKIFGPDKKATQVESS
                     KDFAKKIMAKYNIPTAAYKTFYDLDSAWTYVQEQGAPIVIKEDGLKAGKGVTVASTLE
                     QAKEALNIAFAIENNSVVIEECLFGFEFSLICLVHNETVVPLEVAQDHKCVFDGDKGP
                     NTGGMGSYSPVKKITSEIIDEAMHEIMKPMASAMVKEGVPFTGFLYGGLMLTEKGIKT
                     IEFNARFGDPEAEVILPRLESDFVQAILDLMDNKPVELKWTDKVSHGVVLASTNYPAS
                     STKGSLITGVDDVDGLVFHMGTKMTDEGLVTDGGRVLMVVTLEDDLQTAFDHTYKELE
                     KIQCKDLFHRNDIGKKDM"
     gene            1773..3443
                     /locus_tag="ACZPDN_09070"
     CDS             1773..3443
                     /locus_tag="ACZPDN_09070"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1298812.1"
                     /translation="MAKTDLEIAQECVMEPIEKVAAKIGIPADSLEHYGKYKAKLSFD
                     YLKSIENNPHGKLVLVTAINPTKAGEGKSTTTVGLGDALNRLGKKTMMALREPSLGPV
                     FGLKGGATGGGYAQVVPMEDINLHFTGDMHAITTANNLVSACLDNVIHQGNELNIDPE
                     KVTFKRCLDMNDRTLRNITIGCGAKANGVERKDGFNITVASEIMAALCLADDLMDLKK
                     RFGKMLVAYTYDDKPVYVHDLGIEGALAMVMKDAVLPNVVQTLEHNPVLIHGGPFANI
                     AHGCNSVIATKACLELADYTVTEAGFGADLGAEKFLDIKSRLANLKPNAVVIVATIRA
                     LKQHGGIALEDLKEENVEAMLAGCENLAKHIDTVQQFGLPYIVAINEFATDTPAEVAA
                     LEKWCEENNHPMSLSQVWAKGGEGAIDLANKVVALCDQENNYAPLYDLDKTIEEKIET
                     IAKKVYGADGVDFTEEAQAQIAKFNELGWDKLPICMAKTQMSLSDDGKVMGRPTGFRI
                     TVRELRPSLGAGFIVALTGKVLTMPGLPKHPSALDMDVDENGKIVGLF"
     gene            complement(3530..4321)
                     /locus_tag="ACZPDN_09075"
     CDS             complement(3530..4321)
                     /locus_tag="ACZPDN_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298813.1"
                     /translation="MKKLAIAITSLLLMTGCASTPTITNTNNIKEHILKDNVAYDSFS
                     SYSDSDTIIRLPNGEYIHGTKEVNGKYYDSDQDSGAIQAKKAKYYALLATDVDNYLNE
                     KFEGFNDSDEVFYNNKGSFTNASTVIDENGNETDLANNPDYDSMTIKEAKEKEYNRLI
                     QEDAKEEKKNLSSPVSELNKLLPKTDSISRTVFNKKNKYAIHYYEVEKNEYFDYIKKI
                     KEKGFDSIDPNSPEETFLGVNSDNISVNIHYDATNKTLDVDIRRQ"
     gene            4400..4687
                     /locus_tag="ACZPDN_09080"
     CDS             4400..4687
                     /locus_tag="ACZPDN_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298814.1"
                     /translation="MHNILFRFEKMTDASFKVLYTVETDDLKSFAGISGSVSMTLELN
                     VFISKELQGFLVVDEVKVVAKATKCALLIIIIIAGFGTGMLSPEILGVALS"
     gene            complement(4730..>5227)
                     /locus_tag="ACZPDN_09085"
     CDS             complement(4730..>5227)
                     /locus_tag="ACZPDN_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298815.1"
                     /translation="FPIVAVFLYPSEKLTVFQCVALIALLINFICCLLYHFYFFCAYD
                     ILLFIGLMFYLTIRIRKNIKIPDSISSSSNLNLYTTIKIEITDKEIILILPNKTIIDN
                     LTKYIEFIVIKNGILLITDVQNQKNDIQNLKNVKVIYIPTKKFSSSEKQKLINLFYDN
                     RLKRI"
BASE COUNT         1765 a          710 c         1031 g         1721 t
ORIGIN      
        1 ccgtatgcgg gaaaactgca cgtacggttc ttagaggagt ttatctccaa ctgtttaaag
       61 taaatatgag taaaagaagg aggtaaattt acttatgaaa cttatcctag caattttatc
      121 aaatgacgat agtccagtgg caagtaatgc actaaataaa aatggttttt ctgttactaa
      181 attagcaacg actggtggat tccttcgcgc aggcaataca acgttgatta ttggttgtga
      241 agacgaaaaa gtagaagcag ctattgatat catcaaggaa tattcaagta agcgaacgga
      301 aattgtacct agtacagcta gttatgatat tggccgttat gcaagttttc ctgtggaagt
      361 tactgttggt ggagccacaa tttttgtgct agatgtagca caattcttaa aagtttaaca
      421 aatggagggt ggaaagtctg tttctaccct ttttctgtta aaaatttgtg aataaagacc
      481 cattatggtc ttttttctgt tatgattgaa aggtaaagaa aaaggagatt aaaacgcaat
      541 gaaaatacta gttatcggta agggcggacg tgaacacgca cttgcgtatg catgtaaacg
      601 ttcagcttta tgtgatgagt tgatttgtgc accaggcaat cctggaatgg caaaaattgg
      661 agaatgtgtc gatgtgaaag acaatgatgt agatggtctt gttgcattgg caaaagaaag
      721 aaatgtagat ttaaccatcg taggtcctga ggccacttta gctctaggtg ttgtagatgc
      781 gtttaaaaaa gaaggcctaa aaatttttgg gccagataag aaggcgactc aagtcgaatc
      841 aagtaaggat tttgccaaga aaatcatggc aaaatataat attccaacgg cagcttataa
      901 aacattctat gatttagatt cagcatggac ttatgtacaa gaacaaggag ctcctatcgt
      961 tattaaggaa gatggtttaa aagcaggaaa aggtgttacg gtagcttcta cattagaaca
     1021 ggctaaagaa gccttaaaca ttgcctttgc gattgaaaat aattcagtag ttattgaaga
     1081 atgcttattt ggatttgaat tttctttgat ttgtttagta cataatgaaa cagtagtacc
     1141 tttagaagta gcccaagatc acaagtgtgt atttgatgga gataaaggtc ctaatacagg
     1201 tggtatggga tcttatagtc cagttaagaa gattacttct gaaattatcg atgaggctat
     1261 gcatgaaatc atgaaaccaa tggcaagtgc catggttaaa gagggtgtac catttactgg
     1321 attcttatat ggtggattga tgttgacaga aaaaggtatt aaaacaattg aattcaatgc
     1381 gcgctttgga gatccagaag ccgaagttat tttgcctcgt ttagaaagtg attttgtcca
     1441 agctatttta gatttaatgg ataataaacc agttgaatta aaatggacag ataaagtaag
     1501 tcatggtgtt gttttagcaa gtacaaatta tcctgcaagc tctacaaaag gatctttgat
     1561 tacaggtgtt gatgatgtag atggattggt tttccacatg ggaacaaaaa tgacagatga
     1621 aggattagtc acagatggtg gacgtgtttt aatggtcgtt actctagaag atgacttaca
     1681 gactgcattt gatcatacat ataaagaatt agaaaagatt caatgtaaag atttatttca
     1741 cagaaatgat attggaaaga aggatatgta aaatggctaa aacagattta gaaattgcac
     1801 aagaatgtgt catggaaccc attgaaaagg ttgccgcaaa aattggaatt cctgcagata
     1861 gcttggaaca ttatggaaaa tataaagcta aattgtcttt tgattattta aaatccattg
     1921 aaaataatcc tcatggaaaa ttagtattag tcacagctat taatccgact aaagctggag
     1981 aaggaaaaag tacgacaaca gttggtttag gggatgcctt aaatcgtttg ggtaagaaaa
     2041 caatgatggc tttacgtgaa ccaagtttag gtccagtatt tggattaaaa ggtggagcta
     2101 caggtggtgg ctatgcccaa gttgtaccaa tggaagatat caaccttcac tttacgggag
     2161 atatgcatgc gattacgaca gccaataact tagttagtgc atgcttagat aatgtgattc
     2221 atcagggaaa tgaattgaat attgatcctg aaaaagtcac attcaaacgt tgtttagata
     2281 tgaatgatcg tactttgcgt aatatcacaa ttggttgtgg tgctaaagca aacggtgtag
     2341 aacgtaaaga tggatttaat attacagttg cgagtgaaat catggcagct ttatgtttgg
     2401 cagatgattt aatggattta aagaaaagat ttggaaaaat gcttgtggca tatacttatg
     2461 atgataagcc tgtatatgta catgatctag gtattgaagg tgcattggct atggttatga
     2521 aagatgctgt attgcctaat gttgttcaaa cacttgaaca caatccagtc ttgattcatg
     2581 gtggtccatt tgcgaatatt gcgcatggat gtaactctgt tattgccaca aaggcatgtt
     2641 tagaacttgc tgattataca gttacagaag ctggatttgg tgcagatcta ggagctgaaa
     2701 aattcttgga tatcaaaagt cgattggcaa atcttaaacc aaatgcagtt gtgatcgttg
     2761 caacaattcg tgccttaaaa cagcatggtg gaattgcctt agaagattta aaagaagaaa
     2821 atgtagaggc tatgcttgcg ggatgtgaaa acttagcaaa acatattgat actgtacaac
     2881 aatttggttt accatacatt gttgcgatca atgaatttgc gacagataca ccggctgaag
     2941 tagctgcatt agaaaaatgg tgtgaagaaa acaaccaccc aatgtcatta tctcaagtat
     3001 gggcaaaagg tggagaaggc gctattgatt tagcgaacaa agtagttgcg ttatgtgatc
     3061 aagaaaataa ctatgctcct ttatatgatt tagataagac tattgaagaa aaaatagaaa
     3121 ccattgccaa aaaagtatat ggtgcagatg gtgttgactt tacagaagag gcacaagcac
     3181 aaattgcgaa gttcaatgaa ttaggatggg ataaattacc aatctgtatg gctaagacac
     3241 aaatgtcatt atccgatgat ggtaaagtaa tgggtcgtcc tacaggattt agaattacag
     3301 ttcgtgaatt acgcccaagt ttaggtgctg gcttcattgt tgctctaaca ggtaaagtat
     3361 taacgatgcc aggacttcca aaacatccaa gtgcattaga catggatgtg gatgaaaatg
     3421 gtaagatcgt aggattattc taatgtcaaa ggctggatga aaattccagt ctttttttct
     3481 ttgtattaaa aaagctcaga caactaaatg ttgtttgagc aatttaaatt tattgtcttc
     3541 gaatatctac atcaagagtt ttatttgtcg cgtcataatg tatattgact gatatattat
     3601 cactgttaac gcctaaaaat gtttcttctg gagaatttgg atcaatggaa tcaaaacctt
     3661 tctccttaat tttctttatg tagtcaaaat actcattttt ttctacttca taataatgaa
     3721 tggcatactt attctttttg ttgaaaaccg ttcttgaaat agaatctgtt tttggcaata
     3781 atttgtttaa ttcgcttact ggagaagata aatttttctt ttcttcttta gcatcttctt
     3841 ggatcaaacg attgtattct ttttcttttg cttcttttat tgtcatagaa tcatagtctg
     3901 gattattggc caagtctgtt tcattaccat tttcatcaat gactgtagat gcgtttgtga
     3961 agctaccttt attattatag aaaacctcat ctgaatcatt gaatccttca aatttttcat
     4021 ttaaatagtt atccacatca gtagctagta aagcataata tttcgctttt ttagcctgaa
     4081 tggcacctga atcttgatca ctatcataat attttccatt cacttctttt gttccatgta
     4141 tatattctcc atttggcaaa cgaataattg tatctgaatc actatatgaa gagaatgaat
     4201 cataagctac attatccttt agaatatgct ccttaatatt atttgtgttt gtgatggtag
     4261 gtgtagaagc gcatccagtc attaaaagta aacttgtgat tgcgatagct aattttttca
     4321 tagttatgtc ctgcctttct aatttaagta tactatatta ttttaattat tgcctagata
     4381 tgtataagag gcaaaaataa tgcataacat cttatttaga tttgaaaaaa tgacagatgc
     4441 aagttttaaa gtgttgtata cagtagaaac ggatgattta aaatcatttg caggaatttc
     4501 aggttctgtt tcaatgactt tagaattaaa tgtttttatt tcaaaagaat tacaaggatt
     4561 cctagttgtg gatgaggtaa aagttgtagc caaggctaca aaatgtgcat tattaataat
     4621 tataattatc gcaggatttg gaacaggtat gttatcacca gaaattttag gtgtggcgtt
     4681 atcttaacta ataaagttca aaaggatata acattataaa attatttcgt tatatccttt
     4741 ttagtctatt gtcataaaat agattgatta atttttgctt ttctgatgaa gaaaatttct
     4801 ttgtgggaat ataaattact tttacatttt ttagattttg aatatcattt ttttgatttt
     4861 gaacatcagt tattaaaagt attccatttt ttataactat aaattcaata tattttgtca
     4921 aattatcaat aatagtttta tttggtaaga tgagaattat ttctttatca gtaatctcga
     4981 tttttatagt tgtataaaga ttgagattac tactggatga tattgagtcc ggaattttta
     5041 tgttctttct tatgcgaatg gttaagtaaa acataagccc tataaaaagc aaaatatcat
     5101 aagcacaaaa aaagtaaaaa tgataaagta gacaacaaat aaaatttatt aataaagcaa
     5161 ttaatgccac acattgaaat actgtaagtt tttcagatgg atacaaaaaa accgcaacta
     5221 tggggaa
//
LOCUS       DBMDWL010000141         5189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6507_length_5189_cov_2.359277, whole genome shotgun sequence.
ACCESSION   DBMDWL010000141 DBMDWL010000000
VERSION     DBMDWL010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6507_length_5189_cov_2.359277"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="ACZPDN_09090"
     CDS             complement(<1..250)
                     /locus_tag="ACZPDN_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR1298816.1"
                     /translation="MRTVVIGSGSWGTALAQVLQDNHQEVMVYGVAENEVNDINENHK
                     NSKYFGDVELNPELKATTDIECVKGADIVLLAVPTIAID"
     gene            complement(264..1571)
                     /gene="der"
                     /locus_tag="ACZPDN_09095"
     CDS             complement(264..1571)
                     /gene="der"
                     /locus_tag="ACZPDN_09095"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR1298817.1"
                     /translation="MIRGTVAIVGRPNVGKSTIFNRLIGQRKSIVDDTPGVTRDRIYG
                     EVEWLTQTFRLIDTGGIQIEDQAFAQEINMQVDIAIEEADVIIFLTSAKEGVMTDDTV
                     IARKLQKCKKPVILACNKVDNPEMQMNVYEFYALGLGDPVVLSGAHGIGLGDLLDIVM
                     EKLPEKNLSPYEGITSFCVIGRPNVGKSSLVNSILKEDRVIVSNVEGTTRDAIDTPFH
                     VDDKEYMIIDTAGIRKKGKIYENIEKYSILRALSAIDRSNVVLFLIDGEKGIRDQDKH
                     VAGLAHDAGKGVIIVYNKWDTVEKDEKTMAKIEKEIRAQFLYLNYAPIAFTSALTGSK
                     VNQLIPLIDQVHDACTLRIPTNILNDVILDAQMMNPAKPHLGKQLKIYYASQVAVEPP
                     TIVLFVNDPELLHFSYARYIENKLREAFGFTGTPIKILARKRS"
     gene            complement(1592..2242)
                     /gene="cmk"
                     /locus_tag="ACZPDN_09100"
     CDS             complement(1592..2242)
                     /gene="cmk"
                     /locus_tag="ACZPDN_09100"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008792781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1298818.1"
                     /translation="MKKINIAIDGPSGVGKSTIADIVAEKLGMKHLDTGAMYRCVAYK
                     TLKDNMDIHDENAMSDLMDSIDIHFDEENHVYLDGEDVSKEIRMNDVSMQASKTSQLA
                     IVRKKLVNLQQKVCASKGFIVDGRDICSVVLPDAEVKIFMSASSKARAQRRYDEYISK
                     GINADYETIYQDIVARDYQDTHREISPLQKTEDAIEIDTSNLTIDEVVSKVLEIVE"
     gene            complement(2254..3024)
                     /locus_tag="ACZPDN_09105"
     CDS             complement(2254..3024)
                     /locus_tag="ACZPDN_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298819.1"
                     /translation="MTQLSRKQKYQELRDRLDEETTAAQAQPVKLQRLSRVDSGSHAN
                     KPLYPHDTVRVSPTVKEMPTSPVMEDLLGEVKQYNIDNGNRITDDTQINILKQLDSTS
                     ETRKRNQHVIPMETDDEDLGSTMNISKPTTQEFERPVRQEPKEEKIVLTSDDVEDVKK
                     PVDDNLDLMFLSGDDFDKTEEQQKNEKKKKSKRKKEKRDELESMPSAKMRMKTSDFEK
                     ASKKGKKGSEIVLNVVLGILIIALVAIIGYLVWTFKNI"
     gene            complement(3091..3651)
                     /gene="efp"
                     /locus_tag="ACZPDN_09110"
     CDS             complement(3091..3651)
                     /gene="efp"
                     /locus_tag="ACZPDN_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1298820.1"
                     /translation="MISSNDLKPGLTILVDGEIYTVLEASQNKTARSAMVVKAKVRNI
                     RTGSNVELSWGGGEKVEPAQIEKREMQYLYDTEDAMVFMDNETYDQVEIPMDRLKWER
                     NFIVPNSNVNISSYEGEILGVILPDKATLKIVECEQAVKGDTATSASKNAVLETGLEV
                     KVPLFINQDELIVVNTNDGKYSGRAK"
     gene            complement(3901..4875)
                     /locus_tag="ACZPDN_09115"
     CDS             complement(3901..4875)
                     /locus_tag="ACZPDN_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1298821.1"
                     /translation="MPNIITHTLFAQEIFDKVDEATHDLFEPRLQLLEIGSNGPDFLF
                     FHGMNPKDFYKKSDLRIAGSMFHAAHVNDFYQKALISIRNEQDEDIKKDMMTYVCGHL
                     CHWALDATSHPYVFYRTGPCKGKSAWYHHRFESLLDAIMLKVKRECTIEDFKFYEVCD
                     VTKEQARAIARIYVVAIRQILGFDIKPHQILESLNDWHFIETLFYDASGDKLKALQTL
                     ETFTKAYNSLSGYIVPNHPDDPYDVMNLLHTRWVHPSDSSFVSTESFFDLYDRAQLLA
                     MEAIRLFLAACQNPDLDEDLLLLIKDRNYNLGVSNQDEMTNFDLIFEK"
     gene            complement(4868..>5189)
                     /locus_tag="ACZPDN_09120"
     CDS             complement(4868..>5189)
                     /locus_tag="ACZPDN_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HIR1298822.1"
                     /translation="KEELDAICSRMVDLSKPFEAGLYYNSKMRGHYSLKSILPVFSKD
                     VSYLDLDIQNGLNAVFAYRTYDDKDEEEKNEVKKAISTYCQMDTYAEYIVYHGLIEEV
                     KNNA"
BASE COUNT         1600 a         1078 c          689 g         1822 t
ORIGIN      
        1 gatcaattgc gattgtagga acggccaata atacaatatc ggctcctttt acgcattcaa
       61 tatctgtcgt agcttttaat tcaggattta attctacatc cccaaaatat tttgagtttt
      121 tatgattttc attgatgtca ttcacttcat tttctgcaac accataaacc ataacttctt
      181 gatgattatc ttgtaagact tgagctaaag ctgttcccca gcttccacta ccaatcacaa
      241 ctgtacgcat atttaactcc ttactatgag cgtttacgag ccaaaatttt aattggtgtt
      301 cccgtaaagc caaatgcttc tcttaattta ttttcaatat aacgagcata actaaaatgt
      361 aataattctg gatcattcac aaataataca atcgttggag gttcaactgc aacttgacta
      421 gcataataaa tctttaattg tttacccaag tgtggttttg ccggattcat catctgtgca
      481 tctagaatca catcatttaa aatatttgtt ggaatacgca aagtacatgc atcatgtacc
      541 tgatcaatca aaggaatcaa ttgattcact ttacttccag tcagtgctga agtaaacgca
      601 attggtgcat agttcaaata taagaattga gcacgaattt ccttttcaat cttagccatt
      661 gtcttttcat ctttttcgac tgtatcccac ttattgtaga caatgatgac acctttacca
      721 gcatcatgcg caagaccagc aacatgttta tcctgatcac gaataccttt ttcaccatca
      781 attaagaata atacaacatt actacgatca atagcactta aagcacgtaa gatggaatat
      841 ttttcaatat tctcataaat cttacctttt ttacgaatac ctgccgtatc aatgatcata
      901 tattctttat cgtcaacatg aaatggcgta tcaatcgcat cacgcgttgt accttcaaca
      961 ttggatacaa taacacgatc ttcttttaag attgaattga ctaaacttga ttttccaaca
     1021 tttggacgcc caatgacaca gaatgatgta attccctcat atggagataa attcttttct
     1081 ggaagttttt ccatgacaat atccaataaa tctccaagcc caataccatg agctccacta
     1141 agtacaacag gatctcctag acctaaggca tagaactcat atacattcat ctgcatttct
     1201 ggattgtcca ctttattaca agccaaaata actggctttt tacacttttg tagttttctt
     1261 gcgataactg tatcatcggt cattacacct tcttttgccg aagttaagaa aataataaca
     1321 tccgcttctt caatcgcaat atctacttgc atattgattt cctgggcaaa tgcctggtcc
     1381 tcaatttgaa taccacccgt atcgatcaag cgaaaagttt gtgtcagcca ttccacttca
     1441 ccataaattc tatctcttgt tactccgggc gtatcatcta caatgctctt tctttgtcca
     1501 atcaaacgat tgaatatcgt tgattttcca acgtttggcc gaccaactat ggccacagta
     1561 cctcgaatca tattgtggtc ccctttctac attattcgac tatttctaat acttttgaaa
     1621 cgacttcatc tatagttaga tttgaagtgt caatttcaat cgcatcttct gttttttgta
     1681 aaggcgaaat ttcacgatgt gtatcctgat aatctcttgc gacaatatcc tgatagattg
     1741 tctcataatc tgcattgatt cccttagaaa tatattcatc atatcttctt tgggctcttg
     1801 ccttacttga agcactcata aaaatcttaa cttctgcatc cggcaaaaca acagaacaaa
     1861 tgtctctacc atctacaata aagcctttac ttgcacatac tttttgttga agattcacta
     1921 attttttacg aacgatcgca agctgactcg ttttacttgc ctgcatcgaa acatcattca
     1981 tacgaatttc tttagaaaca tcttccccat ctaaatatac atgattttct tcgtcaaaat
     2041 gaatatcgat cgaatccatt aaatcactca tcgcattttc atcatgaata tccatgttgt
     2101 cttttaaagt cttatatgct acacaacgat acatagcacc cgtatccaaa tgtttcatac
     2161 ctaacttttc agcaacaata tccgcaatcg tactttttcc gaccccacta gggccatcaa
     2221 tcgcaatatt aatttttttc atttcaggcc tcattaaata ttcttaaatg tccaaaccaa
     2281 atatccaata atcgcaacta aggcaataat caatatacct aatacgacat ttaatacgat
     2341 ttctgaaccc ttctttcctt ttttacttgc tttttcaaaa tccgaagttt tcatgcgcat
     2401 ttttgcacta ggcatagatt caagttcatc acgtttttct ttttttcgct ttgacttttt
     2461 cttcttttca tttttttgtt gttcttctgt tttatcaaaa tcatcaccag ataagaacat
     2521 taaatccaaa ttgtcatcca caggtttttt gacatcttct acatcatcac ttgttaagac
     2581 aattttttct tcctttggtt cttggcgaac aggtctttca aactcttgcg ttgtaggttt
     2641 tgaaatattc attgtcgaac ctaaatcttc atcatctgtt tccattggaa tcacatgttg
     2701 atttcttttt cttgtttcac tcgttgaatc caattgtttt aaaatattga tctgcgtatc
     2761 atctgtaata cgatttccat tatcaatatt gtattgtttc acttccccaa gaagatcttc
     2821 cataactgga gaagtaggca tttcttttac agttggagaa acacgcaccg tatcatgtgg
     2881 atacaatggc ttattggcat gtgaaccact atctacacgt gatagtcttt gtagttttac
     2941 tggctgcgct tgagctgcag ttgtttcttc atctaaacga tctctaagtt cttgatactt
     3001 ttgttttctt gaaagttgag tcataagtac ctcctaaaca ttccttacta ttatacacta
     3061 ttcaacaaaa aaagaaaaat aggcaatcgc ctattttgca cgtcctgaat atttaccatc
     3121 atttgtattt actacaatca actcatcttg attgataaat aaaggaactt ttacttctaa
     3181 tcccgtttct aaaacagcgt ttttacttgc agaagtagct gtatctccct taacggcttg
     3241 ttcacactca acgattttta aagttgcctt atccggtaaa ataacaccta aaatttcacc
     3301 ttcataactt gaaatattta catttgaatt tggaacaatg aagtttcttt cccattttag
     3361 acgatccata ggaatttcga cttgatcata tgtttcatta tccataaata ccatggcatc
     3421 ttctgtatca tataaatatt gcatttctct tttttcaatt tgagcaggtt caaccttttc
     3481 tccaccaccc caagacaatt caacattact tcctgtacga atgttacgaa cttttgcctt
     3541 aacaaccatg gcactacgag cggttttatt ttgtgacgct tctaaaactg tatagatttc
     3601 accatcgact aaaattgtaa gacctggttt taaatcattt gaactaatca tgtttttctc
     3661 ctttaaaatt gtatgtttaa cctaaattat tttatatcac gcttgaattt tgtcaagaaa
     3721 aagaaaaaaa gcaccgaagt gctttctttc atcgaaagga tcgataaagt aatttcttat
     3781 gtgttattaa ggaaggatat atacacacaa gatggatttt gagtcgaaag gtgagccata
     3841 ctttctatct cttaccaccc atataatacc cctatttcgt aaacttgtaa acccttccac
     3901 ctatttttcg aaaattaaat caaaatttgt catttcatcc tggtttgaaa cacccaaatt
     3961 ataatttcta tccttgatca ataacaatag atcttcatct agatctgggt tttgacaagc
     4021 cgccaaaaat agacgaatgg cttccatagc caataattga gcacgatcat atagatcaaa
     4081 gaaactttcc gttgaaacaa acgaactgtc actaggatga acccaacgcg tatgtaaaag
     4141 attcattaca tcatatggat catctggatg atttggaaca atatatccag ataaagagtt
     4201 ataggccttt gtgaatgttt ctaatgtttg aagcgccttt aacttatctc cagaagcatc
     4261 atagaacagt gtttcaataa aatgccaatc gtttaaactt tctaaaattt gatgtggctt
     4321 aatatcaaag cctaagattt gacggattgc cacaacataa atacgtgcaa tcgctctagc
     4381 ttgctcttta gttacatcac atacttcata aaatttaaaa tcttcaattg tacactcacg
     4441 cttcaccttc aacatgatcg catccaataa agattcaaag cgatgatgat accaagcact
     4501 ctttccttta catggtcctg tacgatagaa aacataagga tgacttgtcg catccaatgc
     4561 ccaatgacat aaatgcccac aaacataggt catcatatct tttttgatgt cttcatcttg
     4621 ttcattacga attgaaatca aagctttttg gtaaaaatca ttgacatgag ctgcatgaaa
     4681 catgcttcct gcaattctta gatcactttt cttataaaaa tcttttggat tcattccatg
     4741 aaagaataga aaatcaggtc catttgaacc gatttctaaa agttgtagtc ttggttcaaa
     4801 taaatcatgt gtagcttcat ctactttatc aaagatttcc tgtgcaaata aagtatgtgt
     4861 aataatatta ggcattgttt tttacctcct caatcaaacc atgatataca atatattcag
     4921 catatgtatc catttgacaa tatgttgaaa tggctttttt tacttcattt ttctcttctt
     4981 catccttatc atcatatgta cgatacgcaa agacagcatt caaaccattt tgaatatcta
     5041 aatcaagata agaaacatct tttgaaaaga cgggtaatat actctttaaa gaataatgtc
     5101 cacgcatttt tgaattataa tacaatccag cttcaaaagg ctttgataaa tctaccattc
     5161 tagaacagat tgcatctaat tcttcttta
//
LOCUS       DBMDWL010000142         5134 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6617_length_5134_cov_2.450825, whole genome shotgun sequence.
ACCESSION   DBMDWL010000142 DBMDWL010000000
VERSION     DBMDWL010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6617_length_5134_cov_2.450825"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="ACZPDN_09125"
     CDS             <1..686
                     /locus_tag="ACZPDN_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1298823.1"
                     /translation="FNTNRYLIQDNDVLSFLRWLINNTSFSYNSVLIKLSEYVNKMDL
                     ITVGNNRIESSTIDYSKFFEDLKNPVHLQNFEDFLNLMGIECKLVLKELPDGQVELYF
                     DHKKLVPFYSTASSGTLALTNLYQKIVSKAVESSLVYLDEFDAFYHYEMAEKLISFFK
                     TIYPKCQFIMTSHNTNLMANKIMRPDCLFILSSRGTLTALCDATERELREGHNLEKMY
                     ISGEFEKYE"
     gene            679..1692
                     /locus_tag="ACZPDN_09130"
     CDS             679..1692
                     /locus_tag="ACZPDN_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298824.1"
                     /translation="MNDFCVKPRRRGRSLLIVEGKHEKNQLFWLIFKCFPELNIDFDD
                     VWIYGTNIYKLYDDIQKEYGENWVDDDLDIDLPLVISKKENFDTKSYRNEFTNIILVF
                     DYERHDPSFSETKILKMQEIFMDSTDMGKLYLNYPMIESYLHFKGNVDEQYMDRKISV
                     TLRPGSKYKNMVKKESYIMDAIKFPHKIDDVLEKKCHINKQKRIECCESILSLKSNEM
                     KDKLDKILEIVGDVKQGKTLKFLLIDWVSKLNYFNDNMSYWEYMRAVFRNIVFYNIKK
                     AAKIQHVDDTVNDIREQFDCIDLTEILKNQNGVSKDEINGFIWVLSTCVFLIPDYNFN
                     LLQ"
     gene            1966..3324
                     /gene="trkA"
                     /locus_tag="ACZPDN_09135"
     CDS             1966..3324
                     /gene="trkA"
                     /locus_tag="ACZPDN_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR1298825.1"
                     /translation="MKIIVVGDGKVGFAIAKQLNQEGHDITVVENKSSVLSSTMNQLD
                     IVGVQGNGASLDTLMEARVSSSDLLIAATSTDEVNIICCLLAKKLGARHTIARIRNPE
                     YNNTVRLIKDELGLSMSINPEQAAAREILRAIRFSNSIKVSSFAKGRIELAEVKIKEN
                     SPLSNMRICDINRRMKSEVLFVAVQRNGEVIIPNGNTVIYEKDRVTLTGSTTQLEKFF
                     IKVGILSNRTVNEVMIVGGGRITYYLTNMLLELGMDVKIIEINKDKCVNLVEKFPQAT
                     VIHGDGTDHELLLSESLEEMDAFIALTDNDEENVIISMFAQNHGVTHVLPKVNRVSLG
                     FLLDKLGLENSITPKFITANQIVQYVRAMQNTVGSNVESLIKLVDDRLEALEFRVRDN
                     CRFIDVPIKDLNIRKGIIIAYVTHKGVSKVATGDTRIQLSDTVIIISKVAGLRDINDL
                     LS"
     gene            3308..4825
                     /locus_tag="ACZPDN_09140"
     CDS             3308..4825
                     /locus_tag="ACZPDN_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1298826.1"
                     /translation="MIYYLKHNLHKLNWAMIGNVLGMVLIAQACLMVLPVIVDLIYQE
                     GLYDSYLIVIGICLVLGYALTRTRAKVNNYFAKDGMIAVGLAWIVVSFFGGLPFYLSG
                     EIPSFVDCFFEAVSGFTTTGSSILTEVESLKHATLFWRSFTHWIGGMGILVFILAILP
                     KSNDRDMHIMRAEAPGPTIGKLVPRMRQSAMILYTMYMGLTIIQVILLLIGKMPLFDA
                     LCNTFGSAGTGGFAIKNTSIGAYNNSYYEIIITIFMILFGVNFNMYYFLLIKDFKQVF
                     KNEELRAYLGVILFSIVCITINILSMYNFDVLKSIRLASFQVGSIITTTGYSSCDYSV
                     WPQFSKMILFLLTVCGASAGSTGGGVKISRLLIIVKKIKLDIQKLLHPQKVEAITMDG
                     KVVDTEVVNQIMAYFCSFIIIVGILFFLISFNNFDFETTITSVITCIGNVGPGYGLCG
                     PMGNFSMFSDFSKIVLSFAMLIGRLEIYPIIIFLAPVLGVRKVSKSFHTRNKRKI"
BASE COUNT         1609 a          629 c         1061 g         1835 t
ORIGIN      
        1 cttttaacac gaaccgttat ttaatacaag ataatgatgt tttatctttt ttaagatggt
       61 taattaataa tacgtctttt agttataatt cggttttaat taaattatct gaatatgtta
      121 ataaaatgga cttgattact gtgggtaata atcgaattga atcatcaacg attgattaca
      181 gtaaattttt cgaagattta aaaaatccag ttcatttaca aaactttgaa gattttttga
      241 atttgatggg aattgaatgt aaattggttt taaaagaatt gccggatgga caggttgaat
      301 tatattttga tcataaaaag ttagttccat tctatagtac tgcatcaagt gggacgttgg
      361 cattaaccaa tctttatcaa aaaatagtgt ctaaagcagt ggaatcttca ttggtttatt
      421 tagatgagtt tgatgctttt tatcattatg aaatggcaga gaaattaatt tcttttttca
      481 aaaccatata cccaaaatgt caatttatta tgacttcaca taatacaaat ttaatggcaa
      541 ataaaattat gcgcccagac tgtttattta ttctatcaag tagaggtaca ttaactgctc
      601 tttgtgatgc aaccgaaaga gaattacgag aaggacataa tttggaaaaa atgtacatca
      661 gtggagaatt tgaaaagtat gaatgatttt tgtgtgaagc caagaaggcg tggtagaagt
      721 ttactgattg ttgaaggaaa gcacgaaaag aatcaattat tttggcttat ttttaaatgt
      781 ttccctgaat taaatattga ttttgatgat gtgtggattt atggaacaaa tatctataaa
      841 ttgtatgatg acatacaaaa agaatatgga gaaaattggg tagatgatga tctggatatt
      901 gacttacctc ttgtgattag taaaaaagag aattttgata caaaatctta tcgaaatgag
      961 tttacgaata taattcttgt atttgattat gagcgtcacg atccttcatt ttcagaaaca
     1021 aagattttaa aaatgcaaga aatattcatg gattcaacag atatgggtaa attgtatttg
     1081 aattacccaa tgattgagtc ttatcttcat tttaaaggga atgtagacga acaatatatg
     1141 gatcgtaaga tttcggttac tttgcgaccg ggaagtaaat ataaaaatat ggttaagaaa
     1201 gagagctata ttatggatgc gataaaattt ccccataaaa ttgatgatgt gcttgaaaaa
     1261 aagtgtcata taaataagca aaaacgaatt gaatgttgtg aatcaatatt aagtttaaag
     1321 agtaatgaaa tgaaagacaa acttgataaa attttagaga ttgttggtga tgtcaagcaa
     1381 gggaagacat tgaaattctt gcttatagat tgggtgtcaa agcttaacta ttttaatgat
     1441 aatatgtcgt attgggaata tatgagggct gtatttagaa atattgtttt ttacaatatt
     1501 aaaaaagccg ctaaaataca acatgttgat gatactgtaa atgatataag agaacaattt
     1561 gattgtattg atttaacgga aattttgaaa aatcaaaatg gtgttagtaa agatgaaata
     1621 aatggattta tatgggtttt atcaacttgt gtatttttga tacctgacta taattttaat
     1681 ttacttcaat agtttaaata gtgttttaag acgatcgact tgttgtaatt tgtccaataa
     1741 agttggatag tcaaaataga attgtagtaa ggtgtaagta ggatgcttat tttggtaagt
     1801 gtcctttttt gatggtttgt atttcgatat tgataatgtg ttcattagtt gtgatcaata
     1861 tacctattgt gttcattaca agcctttgta gtactagttt ttatacatgt tttgtattat
     1921 acttgagatg attccatatg ggaattcatt ttttttggag gagttatgaa aattattgta
     1981 gtgggtgatg gaaaagttgg gtttgcgatc gcaaaacaat tgaaccagga aggtcatgat
     2041 attacagtgg ttgaaaataa atcgagtgtt ctatcgagta ccatgaatca gttggatatt
     2101 gttggcgtac agggaaatgg tgcgagttta gatactttaa tggaagctcg tgtttcatct
     2161 tcggacttgt tgattgcggc tacatcaacg gatgaggtta atattatttg ttgtttattg
     2221 gctaagaaat tgggtgcaag acatacgatt gcacgtattc gtaatccgga atacaacaat
     2281 accgttcgtt tgattaagga tgaattggga ttgagtatga gtatcaatcc ggagcaggct
     2341 gcggctcgtg aaattttgcg tgcgattcga ttctcaaatt cgatcaaggt ttcttctttt
     2401 gcgaaaggaa gaattgagct tgcggaagtg aaaattaaag aaaattcacc actttcaaat
     2461 atgcgtattt gcgacatcaa tcgtcgtatg aaaagtgaag tgttatttgt ggctgttcaa
     2521 agaaatgggg aagtgatcat tccaaatgga aatactgtca tctacgaaaa ggatcgtgtc
     2581 acattaacgg gttcgactac acagcttgaa aagttcttta ttaaagttgg tattttaagc
     2641 aatcgtacgg tcaatgaagt aatgattgtt ggtggtggaa gaatcacata ctatttgaca
     2701 aatatgttgt tagaactggg tatggatgta aagatcatcg aaatcaataa ggataagtgt
     2761 gtgaatttgg ttgagaaatt cccacaggct acagtcattc atggcgatgg tacagatcat
     2821 gaattattat taagtgaatc tttagaggaa atggatgcgt ttattgcact tacagataat
     2881 gatgaggaaa atgtcattat ttctatgttt gcccaaaatc atggtgtaac gcatgtactt
     2941 cctaaggtaa accgtgtttc tttaggtttc ttgttggata agttggggct tgaaaactcg
     3001 attacgccta agtttattac ggccaatcaa attgtgcaat atgttcgtgc tatgcaaaat
     3061 acggttggat cgaatgttga atctttgatc aaacttgtcg atgataggct agaggcattg
     3121 gaatttagag tgcgtgacaa ttgtcgtttt attgatgtgc caattaagga tctaaatatt
     3181 cgtaaaggga tcatcattgc gtatgtaacg cataagggtg tttccaaagt ggcaacggga
     3241 gatacgcgta ttcaattgtc agatacggtt attattatta gtaaagttgc aggattgagg
     3301 gatatcaatg atttactatc ttaagcacaa tttacataaa ttaaactggg cgatgattgg
     3361 taatgtattg ggtatggttt taattgcgca agcttgccta atggttttac cggtcattgt
     3421 ggatttgatc tatcaagagg gcttgtatga ttcttacttg attgtgattg gaatctgtct
     3481 agttttagga tatgcgctaa cgcgtacacg tgcaaaagtc aataactatt ttgcgaaaga
     3541 tggaatgatt gcggtaggac ttgcgtggat cgttgtatct ttctttggtg gtttaccctt
     3601 ttatttatcg ggtgaaattc cttcttttgt ggattgtttc tttgaagccg tgagtggatt
     3661 tactacaaca ggatcgagta ttttaacgga agttgaaagt ttgaagcatg ctacactttt
     3721 ctggcgtagc tttacacact ggattggtgg tatgggaatt ttagtcttta ttttggcaat
     3781 acttccaaaa tccaatgatc gtgatatgca tattatgcgt gcagaagcac ctggtcctac
     3841 cattggtaag cttgtgcctc gtatgcgtca atcggctatg attttatata caatgtatat
     3901 gggtcttacg atcattcaag tgattttact tttaattggt aaaatgccat tgtttgatgc
     3961 gctttgtaac acatttggtt cagcaggtac gggtggtttt gcgattaaga atacaagtat
     4021 tggtgcctac aacaattcgt attatgaaat catcattacg atttttatga ttttatttgg
     4081 tgttaacttc aatatgtatt atttcctttt aattaaagat tttaaacaag tctttaaaaa
     4141 tgaagagttg cgtgcctatt taggggtgat tttattctct attgtttgta tcacaatcaa
     4201 tattttgtct atgtataatt ttgatgtatt gaaatccatt cgtcttgcga gttttcaagt
     4261 gggttccatc attacaacaa caggatattc aagttgtgat tatagtgtat ggccacaatt
     4321 tagtaagatg atattattct tattaacggt atgtggtgca agtgctggtt cgactggtgg
     4381 tggtgtaaaa attagtcgtc tattgattat tgtaaagaaa atcaagttgg atattcaaaa
     4441 gttattacac ccacaaaaag tagaggccat cacaatggat ggtaaagtag tggatacaga
     4501 ggttgtcaat cagatcatgg cttatttctg tagctttatc atcattgttg gcatcttatt
     4561 cttcttgatt tcctttaata attttgattt tgaaacaacc atcacatccg tcattacatg
     4621 tattggaaat gtgggtccag gatatgggtt gtgtggtcca atgggcaact tctccatgtt
     4681 ctctgatttt agtaagatcg tattatcttt tgcgatgttg attggtcgtt tggagattta
     4741 tccaattatc attttcttgg ccccagtact tggagttcga aaggtttcta aatcatttca
     4801 tacgcgtaat aaaagaaaaa tataattgaa gaataatcat ttgtctggca atgcattaag
     4861 ttaaaataac ggcttgatca atagtttatg attcattgcg tcttcttttt tcggaatatt
     4921 tgaagtataa gattaaatag agatgtatac aaaacttggc aaatgaaaag agcccataca
     4981 aatcatttta tatgatttga tgggcttttt ttatgttatt ttgatttggt ttttgtgttc
     5041 atcatgtcgg agttgtctgt atgaacgacg taagttgctc tgtcgcatct gacaattgtt
     5101 gatgaataat gaattggctt atcttcaaag tcat
//
LOCUS       DBMDWL010000143         5104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6674_length_5104_cov_1.833500, whole genome shotgun sequence.
ACCESSION   DBMDWL010000143 DBMDWL010000000
VERSION     DBMDWL010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6674_length_5104_cov_1.833500"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(122..448)
                     /locus_tag="ACZPDN_09145"
     CDS             complement(122..448)
                     /locus_tag="ACZPDN_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013777.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1298827.1"
                     /translation="MKTKKELPACPVATSVSLIGGKWKLMIIRNLRSRPWRFNELRRD
                     LEGISQKVLTDSLRQMIDDGLVYRNDYHENPPKVYYGLTDLGKEMMPIIDALADFGDY
                     YKTIVD"
     gene            594..947
                     /locus_tag="ACZPDN_09150"
     CDS             594..947
                     /locus_tag="ACZPDN_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006507191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR1298828.1"
                     /translation="MSNYKKTNVGNEGRTELHEALSLTGAEVSINSLPAGASVPFVHS
                     HKNNEEIYGVITGKGTVIIDGDTVEITAGDWLKISPSAKRQFFAASDSSITYICIQVK
                     ENSLDGFTVNDAIIY"
     gene            complement(1110..1322)
                     /locus_tag="ACZPDN_09155"
     CDS             complement(1110..1322)
                     /locus_tag="ACZPDN_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HIR1298829.1"
                     /translation="MKANPILLQKKYARVVELFAKENNVSLEKALDIFYRSKLYQLMS
                     EGVSDMHCMSDEYLVCELEEECKYTK"
     gene            complement(1319..1789)
                     /locus_tag="ACZPDN_09160"
     CDS             complement(1319..1789)
                     /locus_tag="ACZPDN_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HIR1298830.1"
                     /translation="MILYHGSFVVVNQPDLVHSRKNVDFGKGFYVTPIFEQAEKWCNR
                     FKRRGKEGIVSRYTFDEEACKDLNVLKFDAYSEEWLDFILNCRSGQDGTDYDLVIGGV
                     ANDKVFNTVELYFDGFIDKAEAIKRLQYEKPNLQIAFRTTKALSYLKFEGSEKL"
     gene            complement(1786..2025)
                     /locus_tag="ACZPDN_09165"
     CDS             complement(1786..2025)
                     /locus_tag="ACZPDN_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HIR1298831.1"
                     /translation="MKMDKMMCESKELEFAIFCIENIASRLHVDAQKVYVALSEQTNI
                     LKDYIIPEYEVLHTQSKDYIVDDIIDVMHERGVKL"
     gene            complement(2076..2651)
                     /locus_tag="ACZPDN_09170"
     CDS             complement(2076..2651)
                     /locus_tag="ACZPDN_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1298832.1"
                     /translation="MKIWITRHGQTALNKQKLMQGLTDEPLNETGIQQAKEARKKIGD
                     IHFDAVYASPLQRAIQTASIIGNVDQKDVKIDSRIIEVDFGDYELRNYFKLGLKMTSY
                     WTFPELLPNPKSVESVKSMVKRSQSFLKELEKKNYENVLIVCHGGIIRSLCGYLEDRK
                     NGIKWRPKPNNCEIRVYESNHGKHTFIETYK"
     gene            complement(2664..3254)
                     /locus_tag="ACZPDN_09175"
     CDS             complement(2664..3254)
                     /locus_tag="ACZPDN_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR1298833.1"
                     /translation="MQLRQLLLQYEIENNIKHDEAAKRIGIGRATYFRWLKGESTHLK
                     ANTLKKLSDMLDCDVKSILDEEDRIKPIVGSVKAGYDGFPMEDIEGYIELNRHDGKKG
                     DYFLRVRGDSMINAHIYEGDLVFVKQTNEVESGSIAIVLVGEEATLKRVLYKKGLMIL
                     EAANPKIDTKVFTPEEVEELPVKVIGKVLYARRDFD"
     gene            3407..4666
                     /locus_tag="ACZPDN_09180"
     CDS             3407..4666
                     /locus_tag="ACZPDN_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1298834.1"
                     /translation="MQKTYLCIDLKSFYASVECVEQGLDPFKVNLVVADPTRGGGAIT
                     LAATPAIKKLGVSSRGRIFEIDPKIEYMITPPRMHLYMEYSCKVYKVFLEFIAKEDIH
                     VYSIDESFLDVTSYLKLYQVNAKELAKKMLDRIYEKTGLTATVGIGTNLYLAKIAMDI
                     SAKHNADRMAYLNEDLYRQTLWHHTPMTDFWMLGNGTERRLHKLGIYDMYDLCQFPIE
                     ILYKEFGVNAEYIIDHAWGKEPTTIQDIKNYKSKQHSISNSQILFEDYSYKNAFVVMK
                     EMVDQNVLTLTEKHLVTKQISLMIGYSKDCIKPSCGSCKITNCTNSYSILLEEFKRLY
                     IRIVNPNYPIRQIAISFQDVKDEYYYENYDLFTDVEKVEREKKLQETLVQIKHRYGKN
                     AILKGMNALDYATAKKRNTLIGGHNAY"
     gene            complement(4675..5001)
                     /locus_tag="ACZPDN_09185"
     CDS             complement(4675..5001)
                     /locus_tag="ACZPDN_09185"
                     /inference="COORDINATES: protein motif:HMM:NF020438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HIR1298835.1"
                     /translation="MDLIHKKKENRAQIFQSFDSLKGFKEMIQECEVEIVSPRILSQD
                     DYEILNERVHEIQKGKLVTIEYYDRINYKRITGVVSKINLDTQILQIVKTKINLKQIS
                     YIELEE"
BASE COUNT         1607 a          980 c          726 g         1791 t
ORIGIN      
        1 tttcttttta taagcatatg ttttatatat caatagtgta acatattcta tcttaagtat
       61 attgatggaa aagtgaacct tttacatttt tggttcactt tatccgttaa gaggtcctat
      121 attaatcaac aatcgtctta taataatcac caaaatctgc caatgcatca ataatcggca
      181 tcatttcttt tccaaggtct gtaagtccat aataaacttt cggtggattc tcatgataat
      241 catttctata cacaagacca tcatctatca tttgtcttag actatcagtt agtacttttt
      301 gagaaattcc ttctaaatct ctccgtagtt cattgaatct ccatggacga cttcttaaat
      361 ttcttattat catgagcttc catttaccgc ctattaagga cactgatgtt gctactggac
      421 atgccggaag ctcttttttt gtcttcatat gtagtcacct catttcatat acattttgac
      481 acatttattt tattgtgtac agttacaaaa aggtgcgtac tattttttga gtgtgtgaat
      541 gattatacta taatcaagca acaaggttgc aaataaaaat ggaggtcatt caaatgtcaa
      601 actacaaaaa aactaatgtt ggtaatgaag gtcgtacaga acttcatgaa gcactttctt
      661 taactggtgc tgaagtaagt attaattctc ttcctgctgg tgcaagtgtt cctttcgttc
      721 attcccataa aaataatgaa gaaatttatg gggttattac tggaaaagga actgttatta
      781 ttgatgggga tactgtcgaa attactgctg gtgactggtt aaaaattagc ccatcagcaa
      841 aacgtcaatt ctttgctgca agtgattcaa gtatcactta tatttgcatt caggtgaaag
      901 aaaactcact tgacggattc acagtaaatg atgccatcat ttattaaaaa tattgcataa
      961 caacacgaaa tcctcccggc cctgatggtt tggaggattg tttttattaa tcgttttttt
     1021 caggaagatc ataaatcttt tcatcttttg aaatcagttg aacaccttat gcatatgaca
     1081 tccattttct tataatgcat gaaagcaagc tattttgtat acttacattc ttcttcgagt
     1141 tcacaaacta gatattcatc actcatgcag tgcatatcgg atacaccttc actcatgagt
     1201 tgataaagct ttgaccgata aaatatgtct aaagcctttt ccaatgaaac attattctct
     1261 ttcgcaaaaa gttctacaac acgcgcatat ttcttctgca aaagaattgg atttgccttc
     1321 atagcttttc actcccctca aatttcagat atgaaagtgc ctttgttgtt cgaaaagcta
     1381 tttgcaaatt gggtttctca tattgtaacc ttttaatggc ttcggcttta tcaatgaatc
     1441 catcaaaata cagttctact gtattaaata ctttatcatt tgcaacacca ccaataacaa
     1501 gatcataatc tgtaccgtct tgtccgcttc tgcagttcaa aataaaatcc agccattctt
     1561 ctgaataggc atcaaatttc aatacgttta aatctttaca tgcctcttca tcaaatgtat
     1621 atcttgatac aattccttct ttgccacgtc gtttaaatcg gttacaccat ttttctgctt
     1681 gttcgaaaat tggagttacg tagaagccct ttccaaaatc tacattcttt ctagaatgta
     1741 ccaaatccgg ctgattcacc actacaaatg aaccatgata taaaatcata gctttacacc
     1801 cctttcgtgc ataacatcaa taatatcatc cacaatataa tccttacttt gtgtgtgcaa
     1861 cacctcatac tctgggataa tataatcttt caagatgttt gtctgctcgc ttaaggctac
     1921 atataccttt tgtgcatcaa catgaagtct tgatgcaata ttttcgatac aaaaaatcgc
     1981 aaattctaat tctttgcttt cacacatcat cttatccatt ttcatgcact tgtaacctcc
     2041 aacctctatt tcgttttatt tattcttttc atctcttact tatatgtttc aataaatgta
     2101 tgcttaccat gattggattc ataaacacga atctcacaat tgtttggctt tggtctccac
     2161 ttaataccat ttttacgatc ttctagatag ccacataaag aacgaatgat accaccatga
     2221 catacaatca atacattctc ataattcttc ttttcaagtt cctttaaaaa cgattgactt
     2281 cgtttgacca tactcttgac tgattcaacc gacttaggat ttggcaacaa ttcaggaaaa
     2341 gtccaatagg aagtcatttt taatcctaat ttaaagtagt ttctcaattc ataatcccca
     2401 aaatccactt caataatacg tgaatcaatc tttacatcct tttgatctac attgccaata
     2461 atcgatgctg tttgaatcgc tctttgtaga gggcttgcat ataccgcatc aaaatgaata
     2521 tctccaatct tttttctggc ctctttcgct tgttgaattc ctgtctcatt taaaggctca
     2581 tctgtaagtc cctgcattaa tttttgttta tttaaagccg tctggccatg tctagtaatc
     2641 caaatcttca ttcaattact ccctcaatca aagtctctac gagcatataa gacttttcca
     2701 attactttta caggaagttc ttccacttct tccggcgtaa aaacctttgt gtcaatctta
     2761 ggatttgcag cttctaaaat cattaaaccc tttttatata aaacacgctt taatgttgct
     2821 tcctcaccca caagtacaat cgcaatactt ccagattcca cttcatttgt ctgttttaca
     2881 aagacaagat ctccctcata gatatgggcg ttgatcatag aatctccgcg tacacgtaaa
     2941 aagtaatcgc cttttttacc atcatgacga tttaattcaa tatacccctc aatatcttcc
     3001 attggaaaac catcatatcc agcttttaca ctaccaacga taggtttgat tctatcttct
     3061 tcatctaaaa tgcttttgac atcacaatcc aacatatcgg ataatttctt taatgtattg
     3121 gcctttaaat gtgtgctttc cccctttaac caacgaaaat aagtagcacg accaattcct
     3181 atccttttag ccgcctcatc atgtttaata ttattttcaa tctcatattg tagtaataat
     3241 tgtcttaact gcatagttac accaccttta tctatatagt atcaaaaaat aaataaaata
     3301 ccagtgaaaa tttattaact ttttaataaa gtctcaaatc tatcttgaat ttttatttat
     3361 ttttaactat gattttatac gaacgatatg gggtgtggag gtatttatgc agaaaacata
     3421 tttatgtatt gatttaaaat ccttttatgc atccgtagaa tgtgtagaac aaggccttga
     3481 tccattcaaa gtcaatcttg tggtggccga tcctacacga ggtggtggtg ccattacgct
     3541 tgctgcaaca ccagccatta agaaattagg cgtatcgagt cgtggtcgta tctttgagat
     3601 tgatccaaaa attgaatata tgattactcc acctagaatg catctatata tggaatatag
     3661 ctgcaaagtc tataaagtct tcttggaatt tattgccaaa gaagatattc atgtttattc
     3721 catcgatgaa tcttttttgg atgtcacaag ttatttaaag ctctatcaag tcaatgccaa
     3781 agaattggca aaaaaaatgt tagatcgtat ttatgaaaaa actggcctta cagcaactgt
     3841 aggcattgga accaatctgt atcttgctaa gattgcgatg gatatatccg ccaaacataa
     3901 tgccgatcgc atggcctatt taaatgaaga cttatatcgc caaacattat ggcatcatac
     3961 acctatgact gatttttgga tgcttggaaa cggaacagaa agaagattac acaaattagg
     4021 tatctatgac atgtatgact tgtgccaatt ccctattgaa atattatata aagaatttgg
     4081 tgtaaatgcc gaatatatca ttgaccatgc ttggggcaaa gagcctacga ccattcaaga
     4141 cattaaaaat tataaatcga aacaacattc tatatccaat tcccaaattc tttttgagga
     4201 ttattcctac aaaaatgcct ttgtggttat gaaagaaatg gtggatcaaa acgtattgac
     4261 attaaccgag aaacatttgg ttactaaaca aatctcatta atgattggct actccaaaga
     4321 ttgtatcaaa ccaagctgtg gctcatgtaa gattacgaat tgtacaaact cctattccat
     4381 cttattagaa gaattcaaac gactctatat tcgcattgtg aatccaaatt atcccatacg
     4441 tcagattgcg ataagttttc aagatgtgaa agatgaatat tattatgaga attatgattt
     4501 atttacagac gtagaaaaag tagaacgaga aaaaaagcta caagaaacct tagttcaaat
     4561 caaacatcgc tatggcaaga atgccatctt aaaagggatg aatgccttag attatgcaac
     4621 cgctaagaaa agaaacacat tgattggtgg ccataacgca tattagagga gttttcactc
     4681 ctctaattct atgtagctaa tttgttttag attgattttt gtctttacga tttgaagtat
     4741 ttgcgtatcc agattgattt tagaaactac gcctgtgatt cttttataat tgatacgatc
     4801 ataatactca atcgtgacaa gttttccttt ttgaatttca tgcactcttt catttaagat
     4861 ttcataatca tcctgcgata aaattcttgg acttacaatt tctacttcac attcttgaat
     4921 catttctttg aagcctttta aagaatcaaa gctctgaaag atctgagccc gattttcttt
     4981 tttcttatgt attaaatcca tatatatcac atccttataa ggatatcata cgtatttatt
     5041 atataatttc aagatagatt tgagactttc tttagaaatt ttctaaaatg tatttttgcg
     5101 tgtt
//
LOCUS       DBMDWL010000144         5073 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6737_length_5073_cov_2.043443, whole genome shotgun sequence.
ACCESSION   DBMDWL010000144 DBMDWL010000000
VERSION     DBMDWL010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6737_length_5073_cov_2.043443"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="ACZPDN_09190"
     CDS             <1..401
                     /locus_tag="ACZPDN_09190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1298836.1"
                     /translation="IDQRVCHVNGDDLYLYPDQTLIQKFLKQNTKKYIDLRFEFFCEQ
                     LHIHNISLQYGFYKSKWGSCTPSKHKICFNVNLIFMPLEFVDAIILHELAHLFHFNHS
                     KDFYNLLCTWMPNYRQVLKENKRNPIPNLY"
     gene            complement(429..1859)
                     /gene="purB"
                     /locus_tag="ACZPDN_09195"
     CDS             complement(429..1859)
                     /gene="purB"
                     /locus_tag="ACZPDN_09195"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR1298837.1"
                     /translation="MNEYYQSPLSQRYASKEMQALFSNDKKFTTWRRLWIALAESEKE
                     LGLDITEEQIEELKAHALDINYDVAKAREKEVRHDVMSHVYAYGVQCPKAKGIIHLGA
                     TSCYVGDNTDLIIMHEALELVQKKIVNVLEKLEEFALKYKDMPCLAFTHFQPAQPTTV
                     GKRASLWAQDLYMDYVEIQHLLNNKKLLGCKGTTGTQASFMELFEGDTDKVKALDKKI
                     CEKLGYDQYFAVSGQTYTRKYDTQVLQFLVQIAQSAHKFSNDIRLLQHMKEIEEPFEK
                     SQIGSSAMAYKRNPMRSERMAALANYIIADSINPAITASTQWFERTLDDSANKRISIA
                     EAFLATDGLLDLYLNVSDGLVVYPKVVEANLLKELPFMATENIMMDAVKAGGDRQELH
                     ELIRQHSMAAGRVVKEEGKPNDLIDRIAQDPKFGMTKEQILSIMKPENFVGRSVQQTE
                     EFFKDFIDPILAKEKDALGMQAAINV"
     gene            1972..3288
                     /locus_tag="ACZPDN_09200"
     CDS             1972..3288
                     /locus_tag="ACZPDN_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HIR1298838.1"
                     /translation="MKTLSILSTKLSAWGLHLIGRGGSMPGSIGCKVDKNILTKLKFN
                     GPVILVTGTNGKTSTANMITDLFQNAGYNVISNRKGDNLKAGIVTTLLTNCKLNGEIK
                     ANAVVLECDELNVRHILPFIPVTDFVVNNFFRDQLDRAREMEQLIDSIEKVLPDYKGR
                     LILNANDPNVVRLNLVAKNATCSYFGMDENKYSVTTTKEASEGKFCPKCGARLEYSYY
                     QYSHIGKFHCSKCDFKTPEMDVCLKNIDLDQETFTSDKVSFKSPYEGMYSMYNCAVVL
                     NIAKNYGISVDCVKSVFSHAPQPAGRNETFKSSDQTCILNLVKNPTGANEVMKVIEKD
                     DSNKTIVIVLNDREQDGTDVSWIYDTFFEKIMKDSTKEIICTGLRANDMALRIYYGGY
                     KGPLSVVDTLDIAIKAALATKRTTYAIATYTALLPTRNAIKKEMGL"
     gene            3285..4016
                     /locus_tag="ACZPDN_09205"
     CDS             3285..4016
                     /locus_tag="ACZPDN_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR1298839.1"
                     /translation="MKVVWMYHDIMDLYGDKGNMMVLKKRCLDRGIPFELDTCGIGEE
                     KDLSEYDLIFLGGGADKEQISLIPDLLSRKENIKKAMDEKTFVLLICGGYQLFGQYYI
                     AANNEKISGLQFYDYYTDTGKAGSRCIGNVVIDADLDGLKTRIVGFENHGGQTLNVTH
                     PLGKVVKGYGNSFDAGYEGFYDGKILGTYLHGPLLPKNPEVADFVISKAIQKRNPDFK
                     YSDLKPLEDKFETMAREAAFKKLEI"
     gene            4180..5025
                     /locus_tag="ACZPDN_09210"
     CDS             4180..5025
                     /locus_tag="ACZPDN_09210"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIR1298840.1"
                     /translation="MKNLILSIVVILSTLITPISAYELDDIPEYNGTPYVEIHDNEPQ
                     FNSSDMNKKSFESYSNLDSLDRPQVAYANISKDLMPNTKRTSIGTVKPTGWHTVRYKG
                     IDGKYLYNRCHLIGYQLTGENANRKNLMTGTRYLNVEGMLPFENEVCDYIKKTNHHVL
                     YRVTPIYDGDNLVANGVQMEAYSVEDKGQGISFNVYCYNVQPGIKIDYKTGDSSRLKE
                     NIIKEYKASEDNMTYILNVNTHKFHKPTCTSVKKMKDSNKIETKDSRDVLIQQGYLPC
                     KNCNP"
BASE COUNT         1612 a          711 c          974 g         1776 t
ORIGIN      
        1 atatcgatca aagagtttgt catgttaatg gtgatgattt atatttgtat ccagatcaaa
       61 cattgattca aaagttcttg aaacaaaaca caaagaaata tatcgatctt cgttttgaat
      121 tcttttgcga acaattacat atacacaata tttctttaca atatggcttt tataaaagta
      181 agtggggcag ttgtacacct agtaaacata agatttgttt caatgtgaat cttatattta
      241 tgcctttaga atttgtggat gcgattattc ttcatgaatt agcgcattta tttcatttca
      301 atcattcaaa agatttctat aatttacttt gtacttggat gccaaattat agacaggttt
      361 tgaaggaaaa taaaaggaat ccaattccaa atttgtatta aaaaagagct caagtgagct
      421 ctcatttttt atacgttaat agctgcttgc atgcctaatg catctttttc tttagctaaa
      481 attggatcaa tgaagtcttt gaagaattct tctgtttgtt gaacagaacg acctacaaag
      541 ttttctggtt tcatgataga aaggatttgt tcttttgtca tgccaaattt tggatcttgt
      601 gcaatacgat caatcaaatc atttggttta ccttcttctt taaccacacg accagctgcc
      661 attgaatgtt gacgaatcaa ttcgtgtaac tcttgacgat ctccaccggc tttaacagca
      721 tccatcataa tgttttctgt agccatgaat ggtaattctt ttaaaaggtt tgcctctaca
      781 acttttggat atacaactaa accatcactt acatttaagt aaagatctaa tagtccatct
      841 gtagctaaga aggcttcggc tattgaaata cgtttgtttg cggaatcatc caatgttctt
      901 tcaaaccatt gtgtacttgc cgtaattgca gggttgatgg aatcggcaat aatgtagttc
      961 gcaagtgctg ccattctttc tgaacgcata gggttacgtt tgtaggccat tgcgcttgaa
     1021 ccgatttgtg atttttcaaa tggttcttcg atttctttca tatgttgtaa taaacgaata
     1081 tcattactaa atttgtgtgc actttgtgcg atttgaacta agaattgtaa tacttgtgta
     1141 tcatatttac gagtataggt ttgtccactt accgcaaaat attggtcgta acctaatttt
     1201 tcacaaatct ttttatccaa tgcttttact ttgtctgtat ctccttcaaa caattccata
     1261 aagcttgctt gtgttcctgt tgtaccttta catcctaata gttttttatt gtttaataag
     1321 tgttggattt caacgtagtc catatataag tcttgtgccc ataagctagc tcttttacct
     1381 acagttgttg gttgagcggg ttggaagtga gtaaacgcaa gacatggcat gtctttgtat
     1441 tttaatgcga attcttctaa tttttctaat acattgacaa ttttcttttg tacaagctct
     1501 aaagcttcgt gcatgataat caaatctgtg ttgtccccaa cgtagcagct tgttgcccct
     1561 aagtgaataa ttccttttgc ttttggacat tgcactccat atgcatatac gtgtgacatt
     1621 acgtcatgtc gtacttcttt ttctctagct tttgcgacat cgtagttaat atctaaagca
     1681 tgtgctttta attcttcgat ttgttcttct gtgatatcta atcctaattc tttttcagat
     1741 tctgcaagag caatccaaag tcttctccat gtcgtaaatt ttttgtcgtt tgaaaataat
     1801 gcctgcattt ccttagatgc atatctctgt gaaagaggag attggtaata ttcgttcatt
     1861 tgttccacct attctagtta gtttcttcct aacatcaata ttatatgtgt ttcaattttt
     1921 aaattcaact tcaattgttg tataatagaa gagtctgaaa aaggagtact tatgaaaaca
     1981 ttatctatat tatctacaaa actaagtgcc tggggacttc atcttattgg aagaggtgga
     2041 tctatgcctg gatctattgg ttgcaaggtg gataagaata tattaacgaa attaaaattt
     2101 aatggacctg tcattttagt tacgggtacc aatggtaaaa cgagtaccgc aaatatgatt
     2161 acggacttgt ttcaaaatgc aggatacaat gttatttcga atcgtaaagg agataactta
     2221 aaggcgggta ttgtgactac attgcttaca aattgtaagt taaatggtga aatcaaagcc
     2281 aatgcggtcg tgttagagtg tgatgaatta aatgtaagac acattcttcc ttttattccg
     2341 gttacggact ttgtggtcaa caacttcttt cgtgatcaat tagatcgtgc gcgtgaaatg
     2401 gaacagttga ttgattcgat tgaaaaggtt ttaccagatt ataaggggcg tttgattcta
     2461 aatgcgaatg atccaaatgt tgtgcgttta aatttagttg ccaaaaatgc gacttgttct
     2521 tattttggaa tggatgagaa taagtattct gttacaacca caaaagaggc aagtgaaggg
     2581 aaattttgtc caaagtgtgg ggctagactt gaatatagtt attatcaata ttcgcatatt
     2641 ggtaaattcc attgttcaaa atgtgatttt aaaacaccag aaatggatgt ttgtctaaaa
     2701 aatattgatt tggatcaaga aacatttacg agtgataaag tcagctttaa atctccttat
     2761 gaaggaatgt attcgatgta taattgtgcg gttgtattaa atattgcaaa aaattatggt
     2821 atttctgtgg attgtgtaaa atcagtattt agtcatgcgc ctcagcctgc aggaagaaat
     2881 gaaacgttta agtcaagtga tcaaacatgt attttaaact tagttaaaaa tccaacgggt
     2941 gcaaatgagg taatgaaggt cattgaaaaa gatgattcaa ataaaacaat cgtaattgta
     3001 ttaaatgatc gtgaacaaga tggtacagat gtaagttgga tttatgatac tttctttgaa
     3061 aaaatcatga aggatagcac aaaagaaatt atttgtacgg gtctacgtgc aaatgatatg
     3121 gctttaagaa tctattatgg tggttataaa gggcctttaa gtgtggtaga tactttagat
     3181 attgccatta aagcagcttt agctacaaag cgtactacgt atgcgatcgc aacatatacg
     3241 gcattgcttc ctactcgtaa tgcgattaaa aaggagatgg gtctatgaaa gtagtttgga
     3301 tgtatcatga tatcatggat ctttatggtg ataagggaaa tatgatggtt ctaaagaagc
     3361 gttgtttaga tcgtggtatt ccttttgaat tggatacttg tggtattggt gaagaaaaag
     3421 atttaagtga atatgattta atatttttag gtggtggtgc ggataaggaa caaattagtt
     3481 tgattcctga tttactgtcg cgtaaagaaa atattaaaaa ggcaatggat gagaagacgt
     3541 ttgttctttt aatttgtggt ggatatcaat tgtttggtca atattatatt gcggcgaata
     3601 atgaaaaaat ttctggcttg cagttctatg attattacac ggatacgggt aaagctggta
     3661 gtcgttgtat tggaaatgtt gtgattgatg ccgatttgga tggtttaaaa acgcgtattg
     3721 ttggttttga aaatcatggt ggtcaaacat taaacgtaac acatccttta ggtaaggttg
     3781 ttaagggtta tggtaatagt tttgatgcag gctatgaagg tttctatgat ggcaagattt
     3841 taggaaccta tttacatggc ccattacttc ctaaaaatcc tgaggttgcg gattttgtga
     3901 tttcaaaagc aatacaaaaa agaaatccag attttaaata tagtgactta aaaccactag
     3961 aagataaatt tgaaacaatg gctcgtgagg ctgcgtttaa aaaattagaa atataaaaaa
     4021 gatgtcatta gacatctaaa aacatagtac ataaggggtt tattatattt tttgactcga
     4081 acgataattg cgtactatgt tttttaacac tcactccttt catggtttta gtatatgagt
     4141 tatattgaca ttatttatgt cgattatatg aggaatagta tgaaaaattt aattttatca
     4201 attgttgtca ttctttcaac tttgattacg ccaatatctg cttacgaatt ggatgatatt
     4261 ccagaatata atggaacgcc ttatgttgag attcatgata atgagcctca atttaattcg
     4321 agtgatatga acaagaagag ttttgaatcg tattcaaatt tggattcttt agaccggcct
     4381 caagtcgcat atgcgaatat ttcaaaggat ttaatgccaa atacgaaacg tacaagtatt
     4441 ggtactgtaa agccgactgg atggcatacc gttcgttata agggaattga tggaaagtat
     4501 ttatataatc gttgtcattt gattgggtat caattgacag gtgagaatgc gaatcgaaaa
     4561 aatttaatga caggcacacg gtatttgaat gtagagggta tgcttccttt tgaaaacgag
     4621 gtgtgtgatt atataaaaaa gaccaatcat cacgtattat atcgcgttac acctatatat
     4681 gatggtgata atctggttgc caatggtgtt caaatggagg cttattctgt tgaagataaa
     4741 ggtcagggta tatcctttaa tgtatattgt tataatgtgc agccaggtat taaaattgat
     4801 tataaaacag gcgattcaag tcgtttgaaa gagaatatta taaaggaata taaggcaagt
     4861 gaagacaata tgacgtatat tttaaatgta aatacgcata agtttcataa acccacttgt
     4921 acaagtgtaa aaaaaatgaa ggattcaaat aagattgaaa caaaagattc tagagatgtt
     4981 ttgattcagc agggatattt gccttgtaaa aactgtaatc cataggagaa ttaacatgaa
     5041 aaaaataata agtctaatat gtatatgtgt ttt
//
LOCUS       DBMDWL010000145         5052 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6771_length_5052_cov_3.608968, whole genome shotgun sequence.
ACCESSION   DBMDWL010000145 DBMDWL010000000
VERSION     DBMDWL010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6771_length_5052_cov_3.608968"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            130..879
                     /locus_tag="ACZPDN_09215"
     CDS             130..879
                     /locus_tag="ACZPDN_09215"
                     /inference="COORDINATES: protein motif:HMM:NF027486.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4866 family protein"
                     /protein_id="HIR1298841.1"
                     /translation="MPTIFPREEKAEALFDKIAKDPRACKRLMDAFYEGVACDEDSGV
                     EEERVLVDKQVFTNALFTAYKNKDLSAFLMAVCGNSMFDLLRNAFLIPLRFNDKGKEN
                     PILLSDENGNFIESDVHIPESKKHMFKKLVEKNRYLAYGFLEQHSFKEDMSIQTIQNE
                     SHLGILVVQEMPEEVHEKLSDAQIYSGLWDSMMKLEDLLYNAFFYCGDFKGKYCVGVF
                     LPFTHFEHNLEKNIEVSNSILYECIQKMLND"
     gene            complement(891..2201)
                     /locus_tag="ACZPDN_09220"
     CDS             complement(891..2201)
                     /locus_tag="ACZPDN_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR1298842.1"
                     /translation="MNNHMDIPWHEYTNKDSKVKIENASLTEKSSVIGRIGLMLLACG
                     TGAWRVRSSMNTIASELNITCIADIGLTNISYTCIDGIDSHAQSLSLHNTSVNTSKLA
                     RMEDFVYHFKDECKTCTCNEIHNQLDQIESIHSSYSPIILGLAAALACSCFTFLLGGG
                     PIEMLCAFVGAGLGNTLRMKLIKHNYTLFLNVAASVSLACLVYALLFNFLETCFGIAT
                     QHEAGYICSMLFIIPGFPFITSGIDLAKLDLRSGLERLAYAIIIILAATLTAWICALV
                     LHLQPVDFVKLHISTSTKLLLRLLTSFGGVFGFSIMFNSSKKIAASAGCIGAIANTLR
                     LTLVDLSLPAAAAAFIGALTAGLLASMIKGNTGYPRIAITVPSIVIMVPGLYMYRAIY
                     NLELAATMSIGANWLIQSLLIVLALPMGLIFARILTDPSFRYCT"
     gene            complement(2307..2669)
                     /locus_tag="ACZPDN_09225"
     CDS             complement(2307..2669)
                     /locus_tag="ACZPDN_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HIR1298843.1"
                     /translation="MTESEWFEQLFNRLSHSKFRSRFHLKQKEKEYIQAKGMDTIGKH
                     AVDFVEKRLAPAYIPNDGKQTPFKGHPVFIAQHATGCCCRQCLYKWHKIKPNQPLGPR
                     QKAYIVKVLMTWIHKEVD"
     gene            complement(2724..2799)
                     /locus_tag="ACZPDN_09230"
     tRNA            complement(2724..2799)
                     /locus_tag="ACZPDN_09230"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2764..2766),aa:Asn,seq:gtt)
     gene            complement(2920..2995)
                     /locus_tag="ACZPDN_09235"
     tRNA            complement(2920..2995)
                     /locus_tag="ACZPDN_09235"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2960..2962),aa:Asn,seq:gtt)
     gene            3103..3978
                     /locus_tag="ACZPDN_09240"
     CDS             3103..3978
                     /locus_tag="ACZPDN_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR1298844.1"
                     /translation="MLDAIVMDMDGTLLDPNNKILPETKEALIACQKQGTKLVLASGR
                     SYTRLLSYAKELEMDVYGGYLLEVDGIAYYDVQKDERHVLKQMYKEDVEPVFAYLMHK
                     NCETMAVFNDGLFDYIPDPIMEKKKALRNAQEYPEDFPWTGGPWSWLADMRDGYPKIT
                     YIKDLSEIKYPVNKIQTIEDEEVTTQLALDIQNEFKDQYEVFRTCPRELEVLPKGYSK
                     GATLVRMMDMFGWDKDKVFTFGDGENDVSMFGVVTHSFAMGQALDYVKEKAAHVTKSN
                     VEQGIVAALKDFNIL"
     gene            complement(3965..5002)
                     /locus_tag="ACZPDN_09245"
     CDS             complement(3965..5002)
                     /locus_tag="ACZPDN_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR1298845.1"
                     /translation="MQPFDKEYVCDIAKQILNIDSPTGYTKRAIDFMDEEAKKLGYTT
                     GRNQKGNLLIYVDGKDSEHTLGLSAHLDTLGLMVRSIKDNGKLAITKVGGPCLPTLDG
                     EYCKIYTRDGKVYTGTILSCSPSVHVYPDASTLERKEDTMEVRIDEVVHSKADVEKLG
                     ICNGDFICIDTKTQITESGFIKSRFLDDKISATALMGVLKYLKDNNITPQRDLVIMFS
                     TYEEVGHGASCLPEEVEELLAVDMGCIGTDLACTEQDVSICAKDSSGPYDYEMITRLV
                     ELSKENKLNYAVDIYPQYGSDVSAALRGGNDIKGALIGPGVHASHGMERTHYDGVEST
                     MKLVVSYITKY"
BASE COUNT         1566 a          798 c         1036 g         1652 t
ORIGIN      
        1 tattttatag gtgttagtat aatactttta gtctgagtag gcaagccatc aaaaacagat
       61 acaaaaagtg tgttaaagga agacttcttg tatctgtttg cccaaaggac tagaaaagaa
      121 gtgagaatca tgccaacaat ttttcctagg gaagaaaagg cagaggcttt gtttgataaa
      181 attgccaagg atcctagagc ctgtaaacga ttaatggatg ccttttatga aggtgttgcc
      241 tgtgatgagg attcaggtgt agaagaagaa agggtgcttg tagataaaca agtatttacc
      301 aatgccttat ttactgcata caagaacaaa gatctgtcgg catttttaat ggctgtatgc
      361 ggtaatagta tgtttgactt attgagaaat gcatttttga ttccattgcg atttaacgat
      421 aagggaaaag agaatccgat tcttttaagt gatgaaaatg gaaattttat agaaagtgat
      481 gttcatattc ctgaatcaaa gaagcatatg tttaaaaagc ttgtagaaaa aaatcgatac
      541 ttagcttatg gctttttaga acagcatagc tttaaagagg atatgtccat tcaaactatt
      601 caaaatgaat cgcatttagg tatcttagtt gtccaagaaa tgcctgaaga ggttcatgaa
      661 aagttaagtg atgcacaaat ttattctggc ttgtgggatt ctatgatgaa gcttgaagat
      721 ttgttgtata acgccttctt ctattgtggt gattttaaag gaaagtattg tgtaggtgta
      781 ttcttaccat ttacacattt cgaacataat ttagaaaaga atattgaagt ttcaaatagt
      841 attttatatg aatgtataca aaaaatgctg aacgattaag ttcagcattt ttatgtacaa
      901 tatcgaaatg agggatcggt taagatacgg gcaaatataa gtcccattgg aagtgccaaa
      961 acaatcaata aagattgaat gagccagtta gctccaattg acatggtagc tgcaagttcg
     1021 agattataaa tagctcgata catgtatagg cctgggacca taatcacaat ggatggtacg
     1081 gttatggcaa ttctaggata tcctgtattt cctttgatca tagaggcaag tagtccggct
     1141 gttaatgcgc caataaaggc tgcggctgca gcaggtaaag ataaatcgac aagtgttagt
     1201 cgtaatgtgt ttgcgatggc tccaatgcat ccggcacttg cggctatttt ttttgaactg
     1261 ttaaacataa tggagaatcc gaatacgcct ccaaaactgg ttaatagtct taatagtaat
     1321 ttggtggatg ttgaaatgtg tagttttaca aaatcaacgg gttgaagatg taagactaaa
     1381 gcgcagatcc atgcagtaag tgtagcagct agaataatga taatggcata ggcaagtctt
     1441 tcaagaccag aacgaagatc taatttagct aagtcaatac cgcttgtaat gaagggaaag
     1501 cctggaatta tgaaaagcat agaacagatg tatccagctt cgtgttgtgt ggctatgcca
     1561 aaacaggttt ctagaaaatt aaataataag gcatatacaa gacaggctaa ggaaacactg
     1621 gcagcgacat ttaagaatag ggtgtagtta tgtttgatga gtttcattcg aagtgtattg
     1681 ccaagtcctg ccccaacaaa ggcacaaagc atttcaatgg ggcctcctcc aagtagaaag
     1741 gtaaaacaac tacaggcaag ggctgcagct aatcctagaa taatggggga gtagctagag
     1801 tgaatgcttt cgatttggtc taattgattg tgaatctcat tgcatgtgca tgttttacat
     1861 tcgtctttaa agtgataaac aaagtcttcc atacgagcaa gtttagatgt gttgacactt
     1921 gtattatgta aagacaagga ttgggcgtga gagtcaattc catcaatgca tgtgtaggaa
     1981 atatttgtaa gtccaatgtc ggcaatacat gtaatattta attcactggc aatggtgttc
     2041 attgaacttc taactcgcca ggcacctgta ccgcaggcta gaagcatcaa tccaattctt
     2101 cctataacgg aagatttttc tgtaagactt gcattttcaa tcttaacttt tgaatctttg
     2161 ttcgtatatt catgccatgg aatgtccatg tggttgttca taagatcatc tcctatatca
     2221 tttatatata atacacatag tataaatata tattcgatat ttaattccat gaatagtata
     2281 gtcaagaaaa aaaagaagtc aatttcttaa tcaacttctt tatgaatcca cgtcattaat
     2341 actttaacaa tataggcttt ttgtctaggg cctagtggct gattgggttt gattttatgc
     2401 catttgtata gacattgtct gcaacaacag ccagtagcgt gttgagcaat gaatacagga
     2461 tgtcctttga atggtgtttg tttaccatcg ttgggtatat aggctggagc taatcttttt
     2521 tcaacaaaat cgacggcatg ttttccaatg gtatccattc ctttggcttg tatatactct
     2581 ttctcttttt gtttcagatg aaaacggctc ctaaatttgg agtgggataa gcgattaaaa
     2641 agttgttcaa accattcgga ttctgtcata actgctcctt atatataata aaaaaaacca
     2701 gcaacgctgg ttttataatc aagtggcgcc tgaaacaaga ctcgaacttg tgacctgccg
     2761 gttaacagcc ggatgctcta ccgactgagc tattcaggca tttgtttttc acttgactgc
     2821 ctaaacataa taccaaaaga actagagtta tgcaagtact ttttttaatt tttttaaaca
     2881 gacataaaaa aaaccagcaa tgctggtttt ataatcaagt ggcgcctgaa acaagactcg
     2941 aacttgtgac ctgccggtta acagccggat gctctaccga ctgagctatt caggcatttg
     3001 tttttcactt gactgcctgt atataatacc aaaagatgtt gtgttatgca agtacttttt
     3061 ataaaattat gatatattat tttttaaatg gagggatgga ctatgttaga tgcgattgta
     3121 atggatatgg atggtacttt attggatcca aataataaga ttttaccaga aacaaaagag
     3181 gcattaattg cttgtcaaaa gcaggggact aagttagtgc ttgcgagtgg aaggagttat
     3241 acaagattac tttcatatgc taaagaatta gagatggatg tatatggtgg ttatttgtta
     3301 gaggtagatg gtattgcata ttatgatgtt caaaaggatg agcgtcatgt tttaaagcag
     3361 atgtataagg aagatgttga gcctgttttt gcgtatttga tgcataaaaa ctgtgagaca
     3421 atggctgtgt ttaatgatgg attatttgat tatattccag atcctataat ggaaaagaaa
     3481 aaggcattaa gaaatgcaca agaatatcca gaggattttc cttggacggg tggtccttgg
     3541 tcttggcttg cagatatgcg tgatggctat cctaagatta cttatattaa ggatcttagt
     3601 gaaatcaagt atcctgtaaa taagattcag acaattgagg atgaagaggt aactacacaa
     3661 ttggctttgg atattcaaaa tgaatttaaa gatcaatatg aagtattccg tacttgtcct
     3721 agagaacttg aagtattgcc taaaggatat agtaagggag ctactttagt tcgtatgatg
     3781 gatatgtttg gttgggacaa ggataaagtc ttcacatttg gtgatggtga aaatgatgtg
     3841 agtatgtttg gtgttgtaac gcatagtttt gcgatgggtc aggctttgga ttatgtaaaa
     3901 gaaaaagcag ctcatgtgac caagtccaat gtggaacagg gtattgtagc tgctttaaag
     3961 gattttaata ttttgtgata tagctaacaa caagcttcat tgtgctttct acaccatcat
     4021 agtgtgttct ttccattccg tggcttgcat gaacaccagg accaattaag gctcctttga
     4081 tgtcgttacc accgcgtaat gcggcactga catctgaacc atattgtggg tagatatcaa
     4141 cggcataatt aagcttgttt tcttttgata gttcaactag acgtgtaatc atttcgtagt
     4201 cgtatggtcc gcttgaatct tttgcacaga ttgaaacatc ttgctcagta caagcaagat
     4261 ctgtaccgat gcatcccatg tctacggcta ataattcttc gacttcttct ggtaaacaac
     4321 ttgctccgtg acccacttct tcatatgtac tgaacataat gactaagtct ctttgaggtg
     4381 taatgttgtt gtcttttaag tattttagta cgcccattaa agcggtagct gaaatcttat
     4441 catctaagaa tctagattta atgaatccac tttctgtaat ttgtgttttt gtatcaatac
     4501 aaatgaagtc tccgttgcag atacctaatt tttctacatc tgcttttgaa tgcacaactt
     4561 catcaatgcg aacttccatt gtatcttctt ttctttctaa tgttgaagca tctggatata
     4621 cgtgtacact tggtgaacaa gaaagaattg ttcctgtata tacctttcca tcacgagtat
     4681 agatcttaca atattctccg tctaatgttg gtaagcatgg gcctccgact ttagtaatgg
     4741 caagttttcc attgtcttta atggaacgaa ccataagtcc taatgtatct aggtgagcac
     4801 tcaatcctaa tgtgtgttca gaatcttttc cgtctacata gatcaacaag tttccttttt
     4861 ggttacgtcc tgttgtatag cctaattttt tggcttcttc gtccatgaaa tcaatggctc
     4921 tttttgtata gcctgttggt gaatcaatat tcaaaatttg tttggcgatg tcacagacat
     4981 attctttatc aaatggttgc atattttaaa actcctcctg tatgtattat acgcttttat
     5041 acaaattcta aa
//
LOCUS       DBMDWL010000146         4990 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6928_length_4990_cov_3.012068, whole genome shotgun sequence.
ACCESSION   DBMDWL010000146 DBMDWL010000000
VERSION     DBMDWL010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6928_length_4990_cov_3.012068"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="ACZPDN_09250"
     CDS             complement(<1..933)
                     /locus_tag="ACZPDN_09250"
                     /inference="COORDINATES: protein motif:HMM:NF020036.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR1298846.1"
                     /translation="MGNYGEKIYSLGETVTGQTQAMSQAIQKTLENNGGVGIMTGSYD
                     QNLSILSVDNLLLHSTGYTFDTFMEQTKGSLRNFFYDEEDILEQDRFLQLHGTGEAQI
                     IAANGTVNNVRLCKEDVTDEAGRQIWVMSVQVNWDHVNLALLNEAIYSGFWYFDCDEN
                     SEIVNANWSHEFRKMLGYHDTLDFPNKLESWSDLLHPQDKERVMVQLQAAIKDKTNQI
                     KYQVEYRMRMKDNQYQWFRASAEVIRRLDGSASRIAGIFINIDAEKKEIMQTQKSAAF
                     HRAFTKADLCEYYVNLEANTFDTFKVEPSLMTVFE"
     gene            complement(1096..1323)
                     /locus_tag="ACZPDN_09255"
     CDS             complement(1096..1323)
                     /locus_tag="ACZPDN_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298847.1"
                     /translation="MSGVISIMQGMGNNGEYWVRMDGAKMLGDYIMVAADLNKHPRGS
                     IVETSLGQGIVCDTGSFTYTSDTQLDIATNW"
     gene            complement(1483..2247)
                     /locus_tag="ACZPDN_09260"
                     /pseudo
     CDS             complement(1483..2247)
                     /locus_tag="ACZPDN_09260"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2339..2632)
                     /locus_tag="ACZPDN_09265"
     CDS             complement(2339..2632)
                     /locus_tag="ACZPDN_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013677.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1298848.1"
                     /translation="MANKKKQFDQEFKQNAVNYCLTHPELSRKECAKNLGIGDSTLYR
                     WKQEAKANNNQAAFTGSGNLTEAELENRRLKRELKDTQDALEILKKAISILGE"
     gene            complement(2712..2987)
                     /locus_tag="ACZPDN_09270"
     CDS             complement(2712..2987)
                     /locus_tag="ACZPDN_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298849.1"
                     /translation="MFKEVEDSFGNIDRCLKVLSRYVYTHTQIENEKVMSVYDVYHVE
                     KKRSGTNLKHCQLYGNAVYEYHERTKKKNCRYPVYEIMQKDMMLAYK"
     gene            complement(3258..3428)
                     /locus_tag="ACZPDN_09275"
     CDS             complement(3258..3428)
                     /locus_tag="ACZPDN_09275"
                     /inference="COORDINATES: protein motif:HMM:NF017007.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIR1298850.1"
                     /translation="MNENLLSEKKKWVASLLVLFLGMMGIHRFYLGRNASGAVMLVMF
                     VLGVLIPIVGCA"
     gene            complement(3559..4263)
                     /locus_tag="ACZPDN_09280"
     CDS             complement(3559..4263)
                     /locus_tag="ACZPDN_09280"
                     /inference="COORDINATES: protein motif:HMM:NF022779.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HIR1298851.1"
                     /translation="MGKRLLSILGLLIAIVGIVFGGNLVLVIVGCVILGLGQMIKDKK
                     EESTASGMDVIIKEFFEDVQFNPNTHMLNVSDTNIPLPKHDYCSGNGYVQGKYKGLDV
                     ELCTIKLTDVTEVLRDETGLWEKNENQIYEGQWMLCNLDCKFSTWLTIYPHSLLDEQT
                     IKTGNDAFDKKFNLSCGDQEEALKILDSSCLKKIMTLTNGPFRKFALNLNMDGKLYIA
                     THSGHGFENASRDELK"
     gene            complement(4378..>4990)
                     /locus_tag="ACZPDN_09285"
     CDS             complement(4378..>4990)
                     /locus_tag="ACZPDN_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015407.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="X2-like carbohydrate binding domain-containing
                     protein"
                     /protein_id="HIR1298852.1"
                     /translation="DMDDTCNVCGYKRVLPTPVVTYKFIEGANGSWTKNSGKNLGFKV
                     NGEISRFTGVKVDGTLIGNDKYTAVSGSTVVTLKKDYLETLSVGKHTLSVVYTDGECT
                     TEFEVIADTKPTTPEEDKKPGTETEKPKPDVEKPTLPEEGKKPSTETEKPVPSKVDKK
                     ADVKSEKKKSLNTAYSYNMGQWMALLLMSGFVLVLTVFKKKKN"
BASE COUNT         1464 a         1111 c          638 g         1777 t
ORIGIN      
        1 ttcaaagacg gtcatcaggg acggttcaac cttaaaggta tcaaaagtat tcgcttccag
       61 attcacataa tactcgcaca aatctgcttt cgtaaaagcc ctgtggaaag cagcagattt
      121 ttgtgtctgc atgatttctt ttttctccgc atcaatgtta ataaaaatcc cggcaatcct
      181 gctggcagaa ccatccaaac ggcgtattac ttctgccgat gctcgaaacc actggtattg
      241 attgtccttc attctcatgc gatactctac ctggtatttt atctggttcg tcttatcctt
      301 aattgccgcc tgaagctgca ccattactct ttctttatcc tgtggatgca atagatctga
      361 ccaggattcc agtttattcg gaaagtccag agtatcatga tagccaagca tttttcggaa
      421 ttcatgactc cagtttgcat tcacaatctc actgttttcg tcacagtcaa aataccagaa
      481 gccagaatag atagcctcat tgagcagtgc caaatttaca tgatcccagt tgacctgtac
      541 ggacataacc cagatctgtc tgcccgcctc atctgtcaca tcctctttac aaagccgtac
      601 attattcact gtaccgtttg ctgcaatgat ttgtgcttcc cctgttccgt gaagctgcaa
      661 aaaacggtct tgctcaagta tatcttcctc gtcataaaag aagtttctca atgagccttt
      721 tgtctgttcc atgaaagtat cgaatgtata tcctgtacta tgcaacagca gattatccac
      781 agacaaaata gataggtttt ggtcataaga tcctgtcatt atacctactc cgccattatt
      841 ttccagtgtt ttctgtatgg cctgtgacat agcctgggtc tgtcccgtta cagtctcacc
      901 taaagaatat attttttctc cataatttcc catcttataa aaatcctctc ttcgtcttat
      961 gccttcttca tcctactttt atctgtatac agagaacctg tccctgcaaa ttttcttacc
     1021 cattttagca tatatattaa aaaaaacata aaaaaagtag aagcccttat aacttctacc
     1081 tttcactcat atatattacc agtttgtcgc aatatctaat tgagtatctg atgtatatgt
     1141 aaaactacct gtatcacaga cgattccttg tcctaatgaa gtctctacaa ttgatcctct
     1201 aggatgctta tttaaatcgg ctgcaaccat aatataatcc cccagcattt ttgcgccatc
     1261 catacgtacc cagtactcac cattatttcc cattccttgc atgatagaaa taactccact
     1321 catatctaaa ttgaactaac acactctggc gagtgtgggc ttttttttaa tatagcctag
     1381 ttcttctcct gtttcttatt ctatcatagt tttgtcttta ttttttgaaa ggatggtact
     1441 acgtcaagaa aaaggactca taaaatagat aaattttaaa ttctaatgtt ttgaataata
     1501 attattctca tattgatttg gtgattcata accgcaatgg ctatggattc gtattgtgtt
     1561 gtagaatgtt tcaatatatt cgaaacacaa atcatatgca tgatcataat cataaatcaa
     1621 agtacgattt aaccattctc ttttaattac tgaatgaaaa gattctatgc atgcattatc
     1681 ccatggtgtg cctttttttg aataacttag aatcagtcct gccgttaact ttttataaag
     1741 tttgcttgta taatgtactc ctctatccga atgtataatt acaggatcca ctgtttttct
     1801 gcgctttcta gccatctcta tacatttcaa tacttcttcc acttccaagg ttcttgataa
     1861 tgtccatgca attatttttc ttgaatacag atccattaca ctcgtaaggt atacaaatcc
     1921 ttccaaggta aatatatacg ttatatctgt acaccatgcc tcgtttggct tgcttggtga
     1981 aaagtctctt tttaaaatgt tatgcagttt tgttgaaaaa tcactatcaa aggttgtttt
     2041 tacatacggt ttaatgtaac aggctttgat acccatttct cgcatatata cacctacagt
     2101 tctttgtgat attttttcgc ctttcttttg catctctttc gctatctttg gtgcaccata
     2161 aatttgatga ttattatcat atatagtttg aatcgcttgc atcctatttt tcttagatat
     2221 agattgcttg cttggctttc gatgtaaata attgtaatat gcttacatag cactccagtc
     2281 actgaaaagt ctttgtcgac cttagactct tcagaaacaa gacagtacag aacttgtgtt
     2341 attctcccag aatgctgatg gcttttttta ggatttcaag agcatcttgc gtatccttta
     2401 attcacgttt taaacgtctg ttttcaagct cagcctctgt tagatttccc gaaccagtaa
     2461 atgctgcctg attgttattg gctttagctt cttgcttcca tctatataaa gtactatctc
     2521 caatacctaa atttttcgca cattctttac gactaagttc tggatgtgtt aaacaataat
     2581 tgactgcatt ttgtttaaat tcttgatcaa actgtttctt tttatttgcc attttctttt
     2641 gccttccttt gtctttgaat catcctcttt ctattgtacc actggtgtaa attttctctt
     2701 tttgactagt cctatttata tgctaacatc atatccttct gcatgatttc ataaactgga
     2761 taacgacaat tctttttctt ggttctttca tgatattcat ataccgcatt cccatatagc
     2821 tggcagtgtt taagatttgt tccacttctc tttttctcta catgatacac atcatatacg
     2881 ctcatgactt tttcattttc aatctgcgta tgcgtatata cataacgaga caataccttt
     2941 aaacaacgat caatatttcc aaagctgtcc tccacttcct tgaacacaat atcattttta
     3001 tgattccctg ttcccttacc attcttgtcc ttgcaagtcc ccttattaaa ctaatgcata
     3061 tacttttgta cacctgccat tgacttttga ttcatcattt ttttctctta attgttccat
     3121 aattttttac ccttagataa gctttcttat cttcccttca ctatggagac gtaaaagttt
     3181 ttaatttggt tcaaaaaaat aaaaaaagca taaacaaata atttgcctat gccattgatc
     3241 atcaacgaat ctttaaatta tgcgcatcct acaataggaa tcaaaacacc taaaacaaac
     3301 ataactaaca tcactgcacc ggatgcattt ctacctaaat agaatctgtg aatacccatc
     3361 attcctaaga ataataccaa taatgaggct acccattttt tcttttctga tagtaaattt
     3421 tcattcattt tcctttcctt tgacatctat agaatactaa agaatgaaat atataaatca
     3481 aaataattgc cattaaaaat gactctcatt aaagagagcc aattattttc ttctaaatac
     3541 atccatcaaa tccataaatc atttcaactc atccctactc gcattctcaa aaccatgtcc
     3601 actatgagtt gcaatataca gcttaccatc catatttaga ttaagggcaa atttacgaaa
     3661 tggaccattt gtcaaagtca tgattttctt taaacaactc gaatccaata ttttaagagc
     3721 ctcttcttga tccccacaac ttaaattaaa cttcttatca aaagcatcat tacctgtttt
     3781 aatggtttgt tcatccaata aactatgagg atatatagtt agccaagtac taaacttgca
     3841 atccagattg cacaacatcc attgaccttc ataaatttga ttctcattct tctcccacaa
     3901 accagtctca tcacgcaaaa cttctgtcac atctgtaagt ttaatagtac ataactctac
     3961 atctagacct ttatatttac cttgtacata tccattgcca ctacaataat catgcttagg
     4021 caaaggaata tttgtatcac taacattaag catatgagta tttggattaa attgcacatc
     4081 ctcaaagaat tcttttataa tcacatccat accagatgca gtactttcct cttttttatc
     4141 cttgatcatc tgtcctaagc caagaatcac acatccaaca atgaccaaaa caagattacc
     4201 accaaacaca atacccacta ttgcaatcaa caatcctagt attgacaaca atcttttacc
     4261 cataccattc atctcccttc actcccatat taacaagccc acaaatacat ctcaaacaat
     4321 tcactaaatt taccaaaata tagacaaaga aggccaatga tcactggcct tcattcatta
     4381 atttttcttt ttcttaaata cagttaatac caacacaaat ccactcatta ataataaagc
     4441 catccattga cccatattat atgagtatgc cgtattcaaa gatttcttct tttcagactt
     4501 aacatctgct ttcttatcta cctttgaagg tactggcttt tcagtttctg tactaggctt
     4561 cttaccctct tctggcaacg ttggtttttc aacgtccggt tttggttttt ctgtttctgt
     4621 tccaggcttc ttatcttctt ctggcgttgt tggttttgta tctgctatta cctcaaattc
     4681 agttgtacac tcaccatctg tgtaaaccac actcaatgta tgtttaccaa cagacaacgt
     4741 ttcaagataa tccttcttca gtgtaacaac agttgaaccg gaaacagcag tatatttatc
     4801 attaccaatt aaagtgccat caaccttaac acccgtaaat ctagagattt caccattcac
     4861 tttgaaacca agattcttgc cactattctt tgtccaagaa ccattagctc cctcaataaa
     4921 cttgtaagtt actacaggcg taggaagtac tctcttatag ccacaaacat tacaagtatc
     4981 atccatatcg
//
LOCUS       DBMDWL010000147         4982 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6951_length_4982_cov_2.358328, whole genome shotgun sequence.
ACCESSION   DBMDWL010000147 DBMDWL010000000
VERSION     DBMDWL010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6951_length_4982_cov_2.358328"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="ACZPDN_09290"
     CDS             <1..392
                     /locus_tag="ACZPDN_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR1298853.1"
                     /translation="QEDDLYIFCKEKISKYIIDNCTQIGKCTVQFEVCLDYEIIKNNK
                     QEIRVMCSSLRLDSVVAQLSHCSRSEALKKIHAGFVKVNDVVLEQNLQLCNNDFVSIR
                     KTGRFQFKEVVSTTKKGRLVLNFDQYI"
     misc_binding    386..600
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            637..3363
                     /gene="ileS"
                     /locus_tag="ACZPDN_09295"
     CDS             637..3363
                     /gene="ileS"
                     /locus_tag="ACZPDN_09295"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1298854.1"
                     /translation="MDYKETLHMPKTAFEMRGNLTKKEPGFQKRWQDEKLYEKMLERR
                     EGAESFVLHDGPPYANGDIHLGHALNKVLKDVIVKSHFMEGYKTPYIPGWDTHGLPIE
                     TAVTKLGYDRKKMGIAEFRKICYDYALKQVANQKAGFLSLGSIGDYDHPYITLQKGFE
                     KHQIEIFGKMAMKGLIYKGLKPVYWSPSSESALAEAEVEYKDVKSPTIYVKFAVKDGK
                     GVLDTDCNFVIWTTTPWTIPANLGISVNPDFDYSLVDTEKGKLIMLSSMVEELCDKFE
                     VEKRDVLKTFKGKEFEYITCTHPLYPERESLLMCGDHVTADAGTGCVHTAPGFGVDDF
                     NIGMKYGLPVYCNVDAQGCMMDDVGEWLAGQYVEDANKTVTQKLDELGVLLKLQFITH
                     SYPHDWRTKKPIIFRATTQWFASIDQIRDELLEQIHSVSWVPAWGEQRMHNMIADRND
                     WCISRQRAWGVPIPIIYGEDDTPIMDQAIFDHVAKLVGEYGSNVWFERDVKDLLPEGY
                     TNEHSPNGIFRKETDTMDVWFDSGSSHTGAMMDRGLGYPADLYFEGSDQYRGWFNSSL
                     IVGTAVHGKAPYKQVLSHGFVMDEKGVKMSKSQWNAVAPSEITKKYGADILRLWACSV
                     DYQADVSMGQKILKQVSENYRKVRNTLRFLMANLDDQQFTAKDCVNFNELSELDQYIL
                     TELKDAVDYCRKAYNEYRFSDVVTYLTNLMTNELSAYYLDYTKDILYIEKEDAPVRRQ
                     VQTVLYNAVEVMTKLWAPILAHTCEEVNDYMHFDAESIHLTTFKDLELDFDVEKIKAD
                     MEELFKVRKDVFKALENARAQGLIGKSLEAHVVLHVSDAQKEIMDRVLSNAAQWFIVS
                     KVTFTTDELEQFEVCQVKVEKATGHVCPRCWNYTESDNEDGLCDRCNHVLHD"
     gene            complement(3386..4471)
                     /locus_tag="ACZPDN_09300"
     CDS             complement(3386..4471)
                     /locus_tag="ACZPDN_09300"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1298855.1"
                     /translation="MQKNKSILSWLVLSICFLIFISSVIFFVQNKDNETTENLNVTLT
                     DVGFDTPITLNCSCSQADFAKYTKVLRKTFKENNKRFDQYHAYKNMNNLYTLNHEAYD
                     HPIQMDETFIDCLKLAMKMQSENSQFDISQGALLNLWHDARENTQVPPSDDKIQEALK
                     HIDLKNIQINKNEVSYLDSELSIDLGAIAKGYTAQLAKEALNKAGLKNGYINAGGNVV
                     LLGEKEDGSNWKIGIQSPDSSDALVQYETKKATCLVTSGDYQRYFTYKHKKYSHIIDP
                     KTGYPANYVRSVTVITNDSGKADALSTTLFCMSVEDGKKYLSTLDYDVQAIWIVDKDS
                     DVSSDLETKDYKIITTDKIKNRVTLAD"
     gene            4608..>4982
                     /locus_tag="ACZPDN_09305"
     CDS             4608..>4982
                     /locus_tag="ACZPDN_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HIR1298856.1"
                     /translation="MSLFLGPMRQHIPGKKELTEKSELLHLMPKKEVYIPLVAGRTLD
                     FDILVQEGDTVSVGTKLAETKSGFYVPIYSSVSGTYKGIVKRMHNSLRPQNHMVIECD
                     FKQTKVQSFAPLDWQSATREECV"
BASE COUNT         1646 a          720 c          988 g         1628 t
ORIGIN      
        1 tacaagaaga tgatttgtat atattttgta aggaaaagat ttcaaaatat attatcgata
       61 attgtacgca gattggtaag tgtacagttc agtttgaagt atgtttggat tatgaaatca
      121 taaaaaataa taaacaagaa atacgtgtta tgtgcagcag tttacgttta gatagtgtag
      181 ttgcgcaatt gtcacattgt tctagaagtg aagcattaaa aaagattcat gcaggatttg
      241 tgaaagttaa tgatgtggta cttgaacaaa atttacaatt gtgcaataat gatttcgtgt
      301 caatcagaaa aacgggtcga ttccagttta aagaggttgt ttcaaccaca aaaaaaggaa
      361 gacttgtttt gaactttgat caatatatct agctaagata aagacacgcc tttattcgtt
      421 atgattgtag agagcaatga agtttttggt gaaacattgt atgaataaga atttaggtat
      481 cccttttgag ctgatcttac gaaatcatag tagtttgatc cggttgaacc gttatctcat
      541 gaaagtgcat agtgaaaact atgaagtaag gtggtaccgc gtgtttatta cgtccttttt
      601 taagggcgtt tttttatttt gaaaaggaga taaagtatgg actacaaaga aactcttcat
      661 atgcctaaaa cggcatttga aatgcgtgga aatttgacta aaaaagagcc tggtttccaa
      721 aaacgctggc aagatgaaaa attgtatgaa aagatgttag aaagaagaga aggagcagaa
      781 tcatttgtgt tacatgatgg tcctccttat gccaatgggg atatccactt aggacatgcc
      841 ttaaataaag tgttaaaaga tgtaattgta aaatctcact ttatggaagg atacaaaaca
      901 ccatatattc caggatggga tactcatggt ttaccaattg aaactgcagt tactaaattg
      961 ggctatgacc gtaagaaaat gggaattgct gaatttagaa aaatctgtta tgactatgct
     1021 ttaaagcaag tagcgaatca aaaagcagga ttcttaagct taggatcaat tggagattat
     1081 gatcatcctt atatcactct tcaaaagggt tttgaaaaac accaaatcga aatctttgga
     1141 aagatggcta tgaaaggttt gatctacaaa ggattaaaac cggtttattg gtcaccaagt
     1201 agtgaatccg ctttagcgga ggcagaagtt gaatataaag atgttaagag tccaacaatt
     1261 tatgttaaat ttgctgttaa agacggaaaa ggcgtattag atacagattg caacttcgtc
     1321 atttggacaa caacaccttg gactattcct gcaaacttag gaatcagtgt gaaccctgat
     1381 tttgattatt cattagtgga tacagaaaag ggtaaattga ttatgctttc aagcatggtt
     1441 gaagaattgt gtgataagtt cgaagttgaa aagcgtgatg ttttaaaaac tttcaaaggt
     1501 aaagaattcg aatatattac ttgtactcac ccattgtatc cagaaagaga aagtctattg
     1561 atgtgtggtg atcatgtaac agccgatgct ggtactggtt gtgttcatac agcacctgga
     1621 tttggtgtag atgactttaa tattggtatg aaatatggtt taccagtata ttgtaacgtg
     1681 gatgcacaag gttgcatgat ggatgatgtt ggcgaatggc ttgctggtca atatgtggaa
     1741 gatgccaaca aaacagtcac tcaaaaatta gatgaattgg gtgtattatt aaaacttcaa
     1801 ttcatcacgc actcataccc gcatgactgg cgtactaaaa agccaattat tttccgtgct
     1861 acaacacaat ggtttgcgtc aattgatcaa attcgtgatg aattattaga acaaattcac
     1921 tctgtatctt gggtacctgc ctggggtgaa caacgtatgc acaatatgat tgccgatcgt
     1981 aatgactggt gtatttctcg tcaacgtgct tggggtgttc caattccaat tatttatgga
     2041 gaagatgata ctccaatcat ggatcaagcc atctttgatc atgttgccaa attagttggt
     2101 gaatatggat caaacgtttg gtttgaaaga gatgttaaag atttattacc agaaggatat
     2161 acaaatgaac attcaccaaa tggtatcttc cgtaaagaaa cagatactat ggatgtttgg
     2221 tttgactcag gatcaagtca tactggagct atgatggatc gtggcttagg ttatcctgct
     2281 gacttatatt ttgaaggtag tgaccaatat cgtggatggt tcaactcaag cttgatcgta
     2341 ggtactgctg ttcatggtaa agctccatac aaacaagtat tatcacatgg tttcgttatg
     2401 gatgaaaagg gcgttaagat gtctaaatca caatggaatg ccgttgcacc tagtgaaatc
     2461 actaagaaat atggtgcaga tatcttacgt ttatgggctt gtagtgtaga ttatcaagcg
     2521 gatgtttcaa tgggtcaaaa aatcttgaaa caagtaagtg aaaactatcg taaagtacgt
     2581 aatacattgc gtttcttaat ggctaactta gatgatcaac aatttactgc gaaagattgt
     2641 gtaaacttca atgaattgag tgaattggat caatatattt taaccgaatt aaaggatgct
     2701 gtagattact gtagaaaagc ttataatgaa tatcgtttct ccgatgtcgt tacatatttg
     2761 acaaacttaa tgacaaacga attatcagct tattacttag attacacgaa ggatattctt
     2821 tatattgaaa aagaagatgc accagttcgt cgtcaagttc aaactgtact ttataatgcc
     2881 gttgaagtaa tgactaaatt gtgggctcca atcttggctc atacttgtga agaagtaaat
     2941 gactacatgc actttgatgc agaaagtatc cacttaacta cattcaaaga tctagaattg
     3001 gattttgatg ttgaaaagat caaagcggat atggaagaat tattcaaagt tcgtaaggat
     3061 gtcttcaaag cattagaaaa tgcacgtgct caaggtttaa ttggtaaatc attagaagca
     3121 catgtcgtat tacatgtaag tgatgcacaa aaagaaatca tggatcgtgt attaagtaat
     3181 gcagcacaat ggtttattgt atctaaagta acattcacaa cggatgaatt agaacaattt
     3241 gaagtttgtc aggttaaagt agaaaaggct acaggccatg tatgtcctcg ttgctggaac
     3301 tacactgaat ctgataatga agatggattg tgtgatcgtt gtaaccacgt attacatgac
     3361 taatatgtaa atgaaaagag taactctaat cggcaagagt tactctgttt tttattttat
     3421 cggttgtgat gattttataa tcttttgttt ctaaatccga tgaaacatca ctatctttat
     3481 ctacgatcca tatcgcttgt acatcataat caagagtcga tagatatttc tttccatctt
     3541 ctacactcat acagaataat gttgtactta aggcatcggc tttgcctgaa tcgtttgtga
     3601 tgacggttac acttcgaaca tagtttgcag gataaccggt tttaggatca ataatatggg
     3661 aatacttttt atgtttatat gtaaaatatc tttggtaatc tccacttgta actaaacaag
     3721 tggctttttt tgtttcatat tgaactagag catcactaga atctggactt tgaataccaa
     3781 tcttccaatt ggatccatct tctttttcac ctagtaaaac aacatttcct cctgcattga
     3841 tatatccatt ttttaatccg gctttattta atgcttcttt ggcaagttga gcggtatatc
     3901 ctttggcaat ggcacctaga tcaatggata attcagaatc caaataggat acttcatttt
     3961 tattaatttg aatattcttt agatcaatat gttttaaggc ttcttgaatc ttgtcatcac
     4021 taggtggaac ttgtgtgttt tctctagcat catgccacaa atttaatagg gctccttgac
     4081 tgatgtcaaa ttgcgagttt tctgattgca tcttcatagc tagttttaaa caatctataa
     4141 aggtttcatc catttgaatc ggatgatcat aagcttcatg attcaaagta taaaggttat
     4201 tcatattttt ataggcatgg tattggtcga atcgtttgtt gttttcttta aatgtctttc
     4261 gtaagacttt tgtatatttc gcaaaatctg cttgtgaaca tgagcaattt agagtgatgg
     4321 gcgtgtcaaa gccaacatct gttaatgtaa cattgaggtt ttctgttgtt tcattgtctt
     4381 tgttttgaac gaagaatatg acagaactta taaagattag aaaacaaatc gataagacaa
     4441 gccatgataa tatagattta tttttttgca taaaagtatt atatgatgaa ttgcgtttaa
     4501 tgtaaatgta agtacttacc taaattatgg gaaattgtca aaaaattctt gaacttgtga
     4561 aaaaaaaggc ctatgattag aaagtataaa tgggaggtag gatttatatg tcactatttt
     4621 taggaccgat gagacagcat atccctggta aaaaagaatt gacagaaaag tctgaattat
     4681 tacatcttat gcctaaaaag gaagtttata ttcctttagt agcaggacgt actttagatt
     4741 ttgatattct tgttcaagag ggagatactg tatccgttgg aacaaaactg gctgaaacaa
     4801 agtcaggttt ttatgtgcca atttattcaa gtgtttctgg aacttataaa ggaattgtta
     4861 aacgtatgca caattctttg cgtccacaaa atcacatggt gattgaatgc gattttaaac
     4921 aaaccaaagt tcaatcattc gcaccattag actggcaaag tgctacaaga gaagagtgtg
     4981 tt
//
LOCUS       DBMDWL010000148         4974 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_6969_length_4974_cov_2.844654, whole genome shotgun sequence.
ACCESSION   DBMDWL010000148 DBMDWL010000000
VERSION     DBMDWL010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_4974_cov_2.844654"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="ACZPDN_09310"
     CDS             <1..776
                     /locus_tag="ACZPDN_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1298857.1"
                     /translation="VNKVVVKNSEMVKDYSKMKSPRLDLLVEFDDRTMVDLEMQLRQT
                     KDNLPIRFSYYLARLHGLQELEGKYYGELKETIVLVFFNVNLIDNHRMCNTFTLRNEE
                     GLSFVEESQDRMKLRTVEIVKLDLNKPLKDMNEQEKMIYYFLNCHKGMDDSKIKVMIE
                     SDGVIQMLEKRVETISDDGWKKIIEDFQKLHENEERMELQLEIEEAHKQVEEANKQVE
                     QKNQEIKQMIQLMLTNGMSLEFISKSLSKSEDEIRDLLR"
     gene            848..934
                     /locus_tag="ACZPDN_09315"
     tRNA            848..934
                     /locus_tag="ACZPDN_09315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:882..884,aa:Leu,seq:caa)
     gene            957..1043
                     /locus_tag="ACZPDN_09320"
     tRNA            957..1043
                     /locus_tag="ACZPDN_09320"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:991..993,aa:Leu,seq:caa)
     gene            1120..2277
                     /gene="mnmA"
                     /locus_tag="ACZPDN_09325"
     CDS             1120..2277
                     /gene="mnmA"
                     /locus_tag="ACZPDN_09325"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1298858.1"
                     /translation="MKVLVGLSGGVDSAVAAYLLKKQGYDVTCCFMRNWDSFANNDIA
                     GNPTIKDDTCPQEQDYLDALAVANHLDLPLQRVDFIEEYWDNVFKTFLSEYEKGRTPN
                     PDILCNKYIKFDSFFEYAMKQGFDKVATGHYASNAKENGFTYLTRAKDQNKDQTYFLC
                     QVEKSKIAKTIFPLAHLEKPEIRQIAAELNLESVATKKDSTGICFIGERNFRQFLSNY
                     LPSKDGDIVDVNTGKVIARHVGVLYYTIGQRKGLNIDHEKGPWFVVGKDVVKNILYVC
                     RTDQRDLLYSDSCIVKGINWILPSLEEIPTKCTCKFRYRQKDQDIEIERLDDTSVLVK
                     YPQTIASVTEGQEAVFYDGDKCIGGGVIEEVFKDGKNLNQLIMETANGYRG"
     gene            2261..4369
                     /gene="recD2"
                     /locus_tag="ACZPDN_09330"
     CDS             2261..4369
                     /gene="recD2"
                     /locus_tag="ACZPDN_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR1298859.1"
                     /translation="MDIEAKLEYIIFENKANHYVVAGFSELKTYHHFTAAGRIEDPIE
                     DQEYVLEGDYITHPKYGEQFRIDMAKKKLPDNSDAIIHFLCGENFPTIGKKTAESIYD
                     VLGENCLEKIHKDPKILNEVPNLSEKKIAIIKKGIQEFTGFNEAYAKLLKYGLSPRQI
                     QMLLDTYDNVLDVIEEDCFKPYYEVYGFGYKTALKIASAIGLSNEDPRRLDAYIYELA
                     RQLSMATGNTYITFATVFSNVQGVSEELIHDSIDRLVAMQYLYVENTRIYPFTLHEDE
                     VVIAKELKNHLFEVESVDVQDRIQKVEFSLAITYDQEQKDAIQLFFDRSFMILTGGPG
                     TGKTTTVKGILEICKEVYPDSKIQLCAPTGRASKRLAQLSNCDSRTIHSLLQWNLEDN
                     SFGKNEEDPLDVDFIIVDEFSMVDTHLFAQLLKALPSRCRILLIGDEDQLESVGPGKV
                     LEDLIKSNVIDTVHLKKIFRQSNGSGIVTLAKEIREETTCHYEDGVEFIERTTPKIMD
                     ALIDYVKDMDLDSMQILAPMYKGAAGIDEINRQMQVLFNPKSPQKNQMKVGTTIFREN
                     DKVMLLKNLPDDDVYNGDIGTILEIDTKRNVISVDFTNNVVDFSTDFLYYLKHAWCIS
                     VHKSQGNEYQSVFCIVDVNAKNMLEKRLLYTAISRAKKQLYIIGNKQLFETQVKLKQK
                     RVRQTTLQEMIERYADYSNA"
     gene            4347..4604
                     /locus_tag="ACZPDN_09335"
     CDS             4347..4604
                     /locus_tag="ACZPDN_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR1298860.1"
                     /translation="MQIIPMRDLKNTVEVERRCAEENGPVFVTKNGYGRLVVMDIEYY
                     EKTMRKMDEAKSILDGLEDAKSGNTVDGDTAISNIRSKYGI"
BASE COUNT         1632 a          605 c         1167 g         1570 t
ORIGIN      
        1 gtgtgaataa agttgttgta aagaattcag aaatggtaaa agactatagt aaaatgaaaa
       61 gtccacgttt agatctatta gtcgaatttg atgatcgaac gatggtggat ttagagatgc
      121 aattaagaca aacgaaagat aatttgccaa ttcgctttag ttattatttg gctagacttc
      181 atggtttaca agaattggaa ggaaaatatt atggagagtt aaaggaaacg atcgtcttag
      241 tgttctttaa tgtgaatcta attgataatc atagaatgtg taacacattc acattaagga
      301 atgaagaagg tttatctttt gtagaagaaa gtcaagatcg tatgaagtta cgtacagttg
      361 aaatagtaaa gttagattta aataaaccgc ttaaagacat gaatgaacag gagaaaatga
      421 tttattactt tttaaattgc cacaagggaa tggatgatag taaaattaag gtgatgatag
      481 aaagtgatgg ggtgattcaa atgttggaga agcgtgtaga aaccatatcg gatgatggtt
      541 ggaaaaaaat aatagaagat tttcaaaaat tgcatgaaaa tgaagaacga atggaattac
      601 aacttgaaat tgaagaagca cataaacaag tagaagaagc gaataaacaa gttgagcaga
      661 agaatcaaga aataaaacag atgattcaat tgatgcttac gaatggaatg agtttagaat
      721 ttatttctaa gtcattgtct aagagtgaag atgaaattag ggacttattg agatagccac
      781 tttttagtgg cttttttaaa aaatctcttg catgagattc tatcttttgg tataatggtc
      841 aagcaatgcc cgggtggtga aattggtaga cgcagtggac tcaaaatcca ccggtagcga
      901 taccgtgccg gttcgagtcc ggccctgggc accatttaaa atttaatatt tcttttgccc
      961 gggtggtgaa attggtagac gcagtggact caaaatccac cggtagcgat accgtgccgg
     1021 ttcgagtccg gccctgggca ccattttgaa ttgttggatc aagtttttcg ggcttggtcc
     1081 ttttttatga tataattttt gatagttgga ggattacata tgaaagtatt agttggatta
     1141 agtggtggag tagattctgc ggttgcagct tatttattaa agaaacaagg gtatgatgta
     1201 acatgttgtt ttatgcgtaa ctgggatagt tttgcgaata atgatattgc gggaaatcct
     1261 acaattaaag atgatacttg tcctcaggaa caggattatt tagatgcact tgcggttgcc
     1321 aatcatttgg atcttccttt gcagcgtgtc gattttattg aagaatattg ggataatgta
     1381 tttaagactt ttttaagtga atatgaaaag gggcgtacgc caaatccgga tattttgtgt
     1441 aataaatata ttaaatttga ttcatttttt gaatatgcca tgaaacaggg gtttgataag
     1501 gtggctacag ggcattatgc ttccaatgct aaagagaatg gttttacgta tttgacgcgt
     1561 gccaaagatc aaaataagga tcagacttat tttttgtgtc aggttgaaaa gagtaagatt
     1621 gccaaaacaa tttttccttt ggctcatctt gaaaagcctg agattcgtca gattgcggct
     1681 gaattaaatt tggaatctgt tgccactaaa aaggatagta cgggtatttg tttcattgga
     1741 gaaagaaact ttagacaatt cttatcaaac tatcttccta gtaaagatgg agatattgtg
     1801 gatgtaaata caggtaaagt gattgctcgt catgtgggtg ttttatatta tacgattgga
     1861 cagagaaagg gcttgaatat tgatcatgaa aagggtcctt ggtttgtggt tggtaaagat
     1921 gttgtaaaga atattcttta tgtatgtcgt acagatcaaa gagatttatt atattcagat
     1981 tcttgtattg tgaagggaat caactggatc ttgccaagtt tagaagagat tcctacaaag
     2041 tgtacttgta agtttagata tcgccaaaaa gatcaggata ttgaaattga aagattggat
     2101 gatacaagtg tacttgtgaa gtatccacaa acaattgctt ctgtcactga gggtcaggaa
     2161 gctgtattct atgatggtga taagtgcatt ggtggtggtg tgattgaaga agtatttaag
     2221 gatggcaaga atttaaatca gttgattatg gagacggcca atggatatcg aggctaagct
     2281 tgaatatatc atctttgaaa ataaggcgaa tcattatgtg gtggctggct tttcagaatt
     2341 aaagacgtat catcatttca cagcggctgg acgtattgaa gatccaattg aagatcagga
     2401 atatgtactt gagggggatt atataacgca tcctaagtat ggggagcaat ttcgtataga
     2461 tatggctaaa aagaagttgc cggataattc ggatgcgatt attcattttc tttgtggcga
     2521 aaattttcct acgattggaa agaagacggc ggaaagtatt tatgatgtct tgggtgaaaa
     2581 ttgtttagag aagattcata aggatcctaa aattttaaat gaagtgccaa atctttccga
     2641 aaaaaagatt gcgatcatta aaaagggaat tcaggaattt acaggtttta atgaggcgta
     2701 tgccaaattg ttgaagtatg gtttgagtcc tagacaaatt cagatgttat tggatacata
     2761 tgataatgtg ttggatgtga ttgaagagga ttgtttcaaa ccatattatg aagtatatgg
     2821 ctttggttat aagacggctt taaaaatcgc aagtgctata ggtttatcga atgaggatcc
     2881 aagacgcttg gatgcctata tttatgagtt ggctagacaa ttatctatgg caacgggaaa
     2941 tacgtacatt acgtttgcga cagttttttc aaatgtgcaa ggtgtaagtg aagaattgat
     3001 tcatgatagt attgatcgtt tggtggctat gcagtatttg tatgttgaaa atacgcgtat
     3061 ttatcctttt actttgcatg aagatgaggt tgtgattgcc aaagaattga agaatcattt
     3121 gtttgaagtg gaaagtgtgg atgttcaaga tcgtatccaa aaggtggagt tttctttagc
     3181 tatcacatat gatcaagaac aaaaggacgc gattcaattg ttctttgatc gttcctttat
     3241 gattttaacg ggtggtccag gtactggaaa gacaacaacg gtaaagggaa tcttggaaat
     3301 atgtaaagag gtatatccgg atagtaagat tcagctttgt gcaccaacag gaagggcttc
     3361 aaagcgttta gcccaattat ccaattgtga ttcaagaacg attcattctt tattgcagtg
     3421 gaatttggaa gataattctt ttggtaagaa tgaagaggat cctttggatg tagatttcat
     3481 tattgtggat gagtttagta tggtggatac gcatttgttt gcccaattat taaaggcttt
     3541 gccaagtcgt tgtcgaattc ttttgattgg ggatgaagat cagttagaga gtgttggtcc
     3601 aggtaaggtt ttggaggatt taatcaaatc caatgtgatt gatacggtac atttaaagaa
     3661 gatttttaga caatcaaatg gttctggtat tgtaacttta gcgaaagaga ttcgtgaaga
     3721 aacaacttgt cattatgagg atggggtaga gtttattgag cgaacgacac caaaaatcat
     3781 ggatgcgctt attgattatg taaaggatat ggatctagat tctatgcaga tcttagcacc
     3841 tatgtataag ggggcagctg gtattgatga gatcaatcgt cagatgcagg tcttatttaa
     3901 tccaaagagt ccgcaaaaga atcagatgaa ggttggaaca acgattttta gagagaatga
     3961 taaggtcatg ttgttgaaga atcttccgga tgatgatgtc tataatggtg atattggtac
     4021 gattcttgag attgatacga aacgaaatgt aatctcggtt gatttcacaa acaatgtggt
     4081 tgacttctct acggattttc tatattacct aaagcatgcg tggtgtatta gtgtacataa
     4141 atcgcaaggg aatgaatatc agagtgtatt ttgtatagtg gatgtgaatg cgaaaaatat
     4201 gttggagaaa cgtttattgt atacagcaat ttctcgtgct aaaaaacaat tgtatattat
     4261 aggaaataag cagttgtttg agacacaagt taagttaaaa cagaaaaggg tgcgtcaaac
     4321 gacgttgcag gaaatgattg aaaggtatgc agattattcc aatgcgtgat ttgaaaaata
     4381 cggttgaagt agaacgtcgc tgtgcagaag aaaatggtcc tgtgttcgta accaagaatg
     4441 gatatggaag attggttgtt atggacatcg agtattatga aaaaacaatg agaaagatgg
     4501 atgaagcaaa atcaatcttg gatggtttag aagatgcaaa atctggaaat actgtagatg
     4561 gagacactgc aatttctaat attaggagca agtatggcat ctaaatttgg ataacgactg
     4621 accaaaagag cggaatctga tttagatggg attgtctgat atattacagt tgatcttgca
     4681 aatccacagg ctgcatccga cttcgtagat aagttgcagg atactattga agaagcaaga
     4741 gcttttcctg aaagtgattc tcttgttcac aatgaatttt tacaaattta aaatgtgcga
     4801 aagaaattgg ttggaaatta tatcaagtac tatcttctgg atgtaggaga aaatataata
     4861 tacattcttc gtattgtata tggtaaacgg aatttggatg aaattcttaa aaaactgcat
     4921 atttgatcac gtaagcatca gtcatgagtt gattggtgct tttattatgt cgtc
//
LOCUS       DBMDWL010000149         4920 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7105_length_4920_cov_5.852874, whole genome shotgun sequence.
ACCESSION   DBMDWL010000149 DBMDWL010000000
VERSION     DBMDWL010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7105_length_4920_cov_5.852874"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="ACZPDN_09340"
     CDS             <1..888
                     /locus_tag="ACZPDN_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298861.1"
                     /translation="NFAQKGLMESQTDMQKMTYKFAMDTYQNRLLQEQFKAELQNWQG
                     FDAMYDARLKAFSLYNVMPQEVEKNVAQTMSFYASAFRDIAAGKYTLKQTENYGKWLS
                     IQQTFAHAATVQSSAALLQGRAALTNANANASYLKKLGGYYDSLTSGQTMSNDMQRYY
                     TDFMLGKMPIGKAESILRQTPYKHLLDLNIQQNEWSLNKLMQEPDLIRSLSGMYKSET
                     SLTNKRVDSYDTDKIFERGESVTRMFKNVSEGISSFTPKSRFSKSSSVDVGPTPPSSG
                     KSWLDAYRENPNYSPTGSK"
     gene            1713..1892
                     /locus_tag="ACZPDN_09345"
     CDS             1713..1892
                     /locus_tag="ACZPDN_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298862.1"
                     /translation="MSKINSRCFIVEVDGFQYVVKAGKLDEFVCLFLPSVVVINSMTT
                     SPVQWHHNLEWYKPI"
     gene            1905..2114
                     /locus_tag="ACZPDN_09350"
     CDS             1905..2114
                     /locus_tag="ACZPDN_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298863.1"
                     /translation="MTAKDYLIALKVIREIQRKQAYCSGTSQPFLAETLKTIELYCPL
                     DFSKSGGRVTDKTILSCYNGNLFST"
     gene            2117..3670
                     /locus_tag="ACZPDN_09355"
     CDS             2117..3670
                     /locus_tag="ACZPDN_09355"
                     /inference="COORDINATES: protein motif:HMM:NF049463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rolling circle replication-associated protein"
                     /protein_id="HIR1298864.1"
                     /translation="MALFPRCNNPVPVAGRHGLTLVGCHSCVQCRVAAQEHLCKILEV
                     EASKHKYVEFITNTYDDMHLPYIDTSYMYPFGYALRIPNRVIKKYNRKTHSFYYVEDK
                     ISKSFRLTDFSTIDTAGMLRDYYARIDKYYSRFPSRSRGIRNNSIVPILWYDDIRKYI
                     GRLRKWFFKEYGEKIRYYVICEYGTQSFRPHYHLLLFHDSPRARADFRNVRSLPMSTK
                     ENPREICVKLDLAELWVYGDSTSKVTDGNIQEYVSKYLTQHSDFPRVLAKFPQRSFHS
                     VLLGSKNRDETRELFKARDFEALTTDYVINKKGVRRPVSMSDAYYSQFAVRFTGSSCF
                     DVEQTSSLFRSVVYVARRFFASQGEIYDDAHVREFLIWLLKPSTSELYKHLYQFRAVH
                     WYAVSFAKPIYNSTDSVNPLKSLLYAAHHHYTLSSYLGLDFYECLKLRFDFILWKDYQ
                     TMVQYFQNLENDKLFAYENYASMSPYTGTYDFDILKTRSIFQYQVQKANMSYTENIKH
                     RAIVDSYKN"
     gene            3676..>4920
                     /locus_tag="ACZPDN_09360"
     CDS             3676..>4920
                     /locus_tag="ACZPDN_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HIR1298865.1"
                     /translation="MANKVLGMHRLKNKVNRNAFDLSHRHMFTAQVGELLPVFSQWVN
                     PNETFKIGYNGKTRTAALNTDAFTRIRENIQYYFVPFQSLWKYFEQQVNNMTKGDAGQ
                     NISKFADSSTEASKISVKLPYISYVDLAGWLSIMVDHALSALNVYIGSNTSFTTLSAS
                     GFKDFCDSSSSYSDVFVCDGYRYCRAAKLLMALGYGNFDTVIQYDIFALAALYVADGN
                     SWNSTSFSKSDYYISFSKFESSTILNSPNLSIFPLLAYHKICNDHYRNEKWQPFEPWT
                     CNIDYLSPSDNMNAKSFISVSTFTDLMTSIIDLENSNLPIDYFTSVLPRAQYGDESAV
                     SVDTKSSDTVFRIFDSADNTKGVIINSSSYVPNDYLAVANQAPAGSPNGYVNKLRASS
                     GNLVLGFRGTLSGDSSALKLSAL"
BASE COUNT         1367 a          854 c          877 g         1822 t
ORIGIN      
        1 aattttgctc aaaaagggct tatggaatct caaaccgata tgcaaaaaat gacctacaaa
       61 tttgctatgg atacgtatca gaataggtta ttgcaggaac aatttaaggc tgagttacag
      121 aattggcaag gttttgacgc tatgtatgat gcgcgcctta aggcttttag tctttataat
      181 gtcatgccac aagaagttga aaaaaatgtt gctcaaacca tgtctttcta tgcttctgca
      241 tttcgggata ttgctgccgg taaatatact cttaagcaaa cggaaaacta tggtaagtgg
      301 ttatctattc agcaaacatt tgctcatgct gctactgttc aaagttcagc cgcattattg
      361 caaggtcgtg cggcattgac aaatgctaat gctaacgctt cttatctcaa aaaacttggt
      421 gggtattatg actctttaac ctctggacaa actatgtcta atgatatgca gcgatattat
      481 accgatttta tgcttggtaa aatgcctatc ggtaaagctg aaagtatttt gcgtcagaca
      541 ccttataagc atttgcttga tttgaacatt caacaaaatg agtggtcttt aaacaagttg
      601 atgcaagagc ccgatttgat acgttctctt agtggtatgt acaagtctga aacatctctt
      661 actaataagc gtgttgatag ttatgataca gataagattt ttgagcgtgg tgaatctgtt
      721 actcgtatgt tcaaaaacgt ttctgaaggt attagtagct ttacacctaa gtctcgattt
      781 agtaagagtt catctgttga tgttggacct acaccaccat cgagtggcaa atcttggctc
      841 gatgcgtatc gtgaaaatcc taattatagc cctacaggtt ccaaataaca aattaggcgc
      901 aaaggataat tcccttgcgc cttttttatc atatcttttc tactttgatt aatatttcta
      961 ttacttctct gcttccctca gctgtcttct tgcttttgta gtaatatagg cttctaactg
     1021 ttacttctgt cgcgtatcct gttgtacgaa ttcttttcga cactactaaa taaattagtt
     1081 aaatatctgt taattgtacg ttaaataatg caaacacgtt ttcagataat tctcattcaa
     1141 cataaatacc ttctgtaaaa atccatattt ttttatgaaa taataaaaat tttatatatc
     1201 tctattaatg tatctgtttt gtatttgtgt gtgtgcgcat ttaatacgta cgtacacaat
     1261 ttaactaaac agatatatta ctttggtttg tcttttagaa aaaccttagc tttgcgcgga
     1321 aaaatctgta acatcttact aactaccttt ttatgaaaat tttccccgaa aattcctact
     1381 atcttgtttg aggtaggcaa aagtgggaat acacttattc ctaattattg cacttttgtt
     1441 ccttgtaact atattcttaa atattgttaa caaatttggt agttccaaaa ctttattcta
     1501 tctttgcacc atgaaaaatg aatttattaa aattatcatc aaagttgcat tgtatgcact
     1561 tggattgata gctgcttatt ttggtgtctc atccatgaca tcatgcagta cgtctcataa
     1621 tgtagctgct cgtggtcgca ctactattat ctcagttgat accacaattg ttaaacataa
     1681 cggctttgtc cgttctaaaa attacaaaac ttatgagtaa aattaattct cgctgtttca
     1741 ttgttgaagt cgatggcttt cagtatgttg ttaaggctgg taaacttgat gaatttgttt
     1801 gtctgttttt gccttctgta gttgtaatta attctatgac tacttcgcct gttcaatggc
     1861 accataattt agaatggtat aaaccaattt aaagcttttt gcttatgact gccaaagatt
     1921 atttgatcgc tcttaaggtt attcgtgaga ttcaacgcaa acaggcttat tgcagtggta
     1981 cttctcaacc ttttttggct gaaactttga aaactataga actttattgt cctttagatt
     2041 tttctaaaag tggtggtcgt gttacagaca aaactatcct tagttgttat aacggtaatt
     2101 tatttagtac gtaattatgg ctctttttcc tcgttgcaat aatcctgttc ctgttgctgg
     2161 tcggcatggt ttaactcttg tcggttgtca ctcttgtgtt cagtgtagag tagctgcgca
     2221 ggaacatctt tgcaaaattt tagaagttga ggcatctaaa cataaatatg ttgaattcat
     2281 taccaacacg tatgacgata tgcatttacc ttacatagat acttcttata tgtatccttt
     2341 tggttatgct ttacgcattc caaatcgtgt tattaagaaa tataacagga aaactcatag
     2401 tttttactat gtagaggata aaatttctaa gtcgtttcgt ctgacagatt tttctactat
     2461 tgatactgcc ggtatgttgc gagattacta cgcacgtatt gataaatatt atagtagatt
     2521 tccttctcgt tcacgtggta tacgaaataa ctctattgtt cctatactat ggtatgatga
     2581 tattagaaaa tatataggtc gtttaagaaa atggtttttt aaagaatatg gtgaaaaaat
     2641 tcggtactac gttatttgcg agtacggtac acaatcattc cgtccgcatt atcatctcct
     2701 attattccac gactcgccta gagcacgcgc agattttagg aatgttcggt ctttgccaat
     2761 gtcaaccaaa gaaaatcccc gagaaatttg tgttaaactc gatttggctg aattatgggt
     2821 ctatggtgat tcgacttcaa aggttacaga cggcaacatc caagaatatg ttagtaagta
     2881 tcttacacaa cattctgact tccctcgagt gcttgctaag tttccacaaa ggagctttca
     2941 ctcagtcctt ttgggttcaa agaaccgaga cgagactcga gaattattca aggctagaga
     3001 tttcgaagca cttacaacag attatgttat taacaaaaaa ggtgtccgac gccctgtttc
     3061 catgtcagat gcgtattact ctcaatttgc cgttagattt acaggttctt cctgttttga
     3121 tgttgaacaa acttcttccc tttttcgttc ggtggtatat gttgcccgcc gatttttcgc
     3181 ttcacaaggt gaaatctacg atgacgcaca tgtaagggaa ttcttgattt ggttgttgaa
     3241 accttcgact tctgagttat ataaacattt gtatcagttt cgggctgttc attggtatgc
     3301 tgtatctttt gctaaaccca tttataatag taccgattct gtaaatcctt taaaatcatt
     3361 gctttatgct gcacatcatc actatacgtt atcatcctac ttaggtttgg atttttatga
     3421 atgtcttaaa ttacgttttg acttcatatt atggaaagat tatcaaacta tggttcagta
     3481 tttccaaaac ttggaaaatg ataagttgtt tgcttatgaa aattatgcta gtatgtcgcc
     3541 ttatactggt acttatgatt ttgatatttt aaaaactcgc tctatttttc agtatcaagt
     3601 ccaaaaagct aatatgtctt atactgagaa tattaaacat agggctattg ttgattctta
     3661 taaaaattaa ttattatggc taataaagtt ttaggtatgc accgccttaa aaataaggtg
     3721 aataggaatg cgtttgattt gtctcaccgt catatgttta ctgcacaagt cggcgagctg
     3781 cttcctgtat tttctcagtg ggtaaatcct aatgagactt ttaagattgg ttataacggc
     3841 aaaacccgta ctgctgcact taacaccgat gcgtttactc gtattcgtga aaatattcag
     3901 tactattttg ttccttttca gtcactttgg aagtactttg aacaacaggt taacaatatg
     3961 actaagggag atgcaggtca gaatatctct aagtttgcag atagttctac tgaggcttct
     4021 aagatttcag ttaaacttcc atatatttct tatgttgatt tggctggttg gttgagcatt
     4081 atggtcgacc atgcattgtc tgctttgaat gtttatattg gttcaaacac ttcttttact
     4141 acgctttctg cttccggttt taaggatttt tgtgattctt cttcgagtta ttcggacgtt
     4201 tttgtttgtg atggttatcg ttattgtcgt gctgctaaac ttcttatggc tttaggttat
     4261 ggcaattttg atactgttat ccagtatgat atttttgctt tggctgcgct ttatgttgct
     4321 gacggcaatt cttggaattc tacatctttt tctaaatcgg actactacat ttcttttagt
     4381 aagtttgagt cgtctactat tttgaatagt cctaaccttt ccattttccc gttgttggct
     4441 tatcataaga tttgtaacga ccattataga aacgagaaat ggcagccttt tgaaccgtgg
     4501 acttgtaaca ttgactattt gagcccgtca gataatatga atgctaaatc tttcatatct
     4561 gttagtacgt ttaccgattt gatgacttct attattgatt tggaaaattc taatcttcct
     4621 atcgattatt ttacgtctgt acttcctaga gctcaatatg gtgatgaaag cgccgtttct
     4681 gtagatacga aaagttcaga tactgtcttt agaatttttg attctgctga caatactaaa
     4741 ggtgttatta ttaattcttc ttcttacgta cctaatgatt atttagcggt tgctaatcag
     4801 gcacctgccg gttcacctaa cggttatgtt aataagttgc gtgcttcttc gggtaatctt
     4861 gtacttggtt ttagaggtac tttatcgggt gattcttcgg ctttgaaact ttctgctctt
//
LOCUS       DBMDWL010000150         4858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7254_length_4858_cov_2.315325, whole genome shotgun sequence.
ACCESSION   DBMDWL010000150 DBMDWL010000000
VERSION     DBMDWL010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7254_length_4858_cov_2.315325"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="ACZPDN_09365"
     CDS             complement(<1..184)
                     /locus_tag="ACZPDN_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013980.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HIR1298866.1"
                     /translation="MEYILHKKSNCKRIKIRVVKGVVCVSAPLNVSTREIDDFVKEQS
                     AWIKNQLSKYTLSKEND"
     gene            complement(184..1017)
                     /locus_tag="ACZPDN_09370"
     CDS             complement(184..1017)
                     /locus_tag="ACZPDN_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR1298867.1"
                     /translation="MSCEGCPSQGKCGKSSETCGIQSNPDNHIKNIITVMSGKGGVGK
                     STVTVMLAKAMAKKGLKVGIMDADITGPSIPRLLALSNEHAFGNEKQQLIPVESKEGI
                     KVMSLNFLVQSESDPVIWRGPVIGGVVKQFYTDVVWGDLDVLLIDMPPGTGDIALTIM
                     QSIPVQGVVMVSTPQPMVSMIVEKACHMCEKMEVKVLGIIENMAYLECPNCHERIEFY
                     KKEDVETLCNSTCTKLYGTLPMLDLIRDINEYDAYSKDQQEKVDAYLSDIANEILEDI
                     A"
     gene            complement(1021..1923)
                     /locus_tag="ACZPDN_09375"
     CDS             complement(1021..1923)
                     /locus_tag="ACZPDN_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1298868.1"
                     /translation="MELKDAFEDYHLYLSVVQRKSKKTVQSYLHDIEVYLDFLKDRKI
                     SKCENISVLDIEAFLDFYAENHVASSLNRMIASIHSFHLQITTMHPEIKDVSLLIHGM
                     KSSKHLPVYLSVDEVKKVFSSFQDNEIDQYNKTILVVLYSCGLRVSELCDLKLNDVHL
                     TEQILKVKGKGDKERIIPISSYCVSQMKYYLDSIRLVWDVKNVSYFFVNRLGHICTRQ
                     YVHLMIKQKVNELNLNPHISAHSFRHSFATHLLDGDADLRIVQELLGHSNISTTQIYT
                     HIQDKRLTSVYDRCFQKIEKSKEE"
     gene            complement(1944..2597)
                     /locus_tag="ACZPDN_09380"
     CDS             complement(1944..2597)
                     /locus_tag="ACZPDN_09380"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF014442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1298869.1"
                     /translation="MSLTLDYFIHEDLKLYQDSEHFRFNTDTKLLAQFVRLKKQERIL
                     DIGTNNGALLVYFDQFHIKELVGVEVLEETSKVAQKNADCFIKHPVKIVNQPIQEYVD
                     DLFDVVVSNPPYFKMNANQKPEHLNFRHLGRMEENLTLEQLIFHANRLLKSYGRFYMV
                     HRPNRLNEINKVLYDNNFSIRNLRFAYDHRDHKVKSVLIEAIKESNCDMNVLEPIYI"
     gene            complement(2594..3763)
                     /gene="dinB"
                     /locus_tag="ACZPDN_09385"
     CDS             complement(2594..3763)
                     /gene="dinB"
                     /locus_tag="ACZPDN_09385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR1298870.1"
                     /translation="MARVILHIDLNAFFASCEEIKNPDLKEFPVAVGSTSKRGVISTA
                     NYEARKYGVHSAMPVYEALRLCPDLVLIQGDYGYYRSMSAQFFAYLKTFTHQIEPASI
                     DECYMDVTDIIKRYKRPLDLVFEIQNGVYEKCHLNCSIGVAPNRFLAKMASDMRKPKG
                     ITVLRKSELSTKLWPLKINEMHGIGKKSVPVLNEMGIYTIGDFADEKNEQKILRKLGK
                     SSYSLIQKARGNSSAALCYSTTQKSISISRTYSNDLYSIDEVSSNLQIIVNELTHKMQ
                     RENQKGRLVTLTLRDTAFHNVCHSMNLSTYSNTYPMIYGACMQLLEAYFEPIGYRYIG
                     VHVGSLKDAKQIVEQVDLFEVPQENTDFILNRLNAKMDSKCFIKASDLLKKEGSN"
     gene            complement(3756..>4293)
                     /locus_tag="ACZPDN_09390"
     CDS             complement(3756..>4293)
                     /locus_tag="ACZPDN_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1298871.1"
                     /translation="ESKVMEHCKDLMLENARFKISCTEKKPSSNGIDEIEFLVSMNPG
                     QDFSSLSVSASGGELSRLMLALKVVFQASNGIETIIFDEIDTGVSGKVALAMGAKMKA
                     LSNKYQVLCITHLASVAVMANTHYLVSKKSTFNETITRVQKLDHDQTIQELAIMTSGE
                     ASVKAKESMQDLWVKIHG"
     assembly_gap    4294..4303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4304..>4858)
                     /locus_tag="ACZPDN_09395"
     CDS             complement(<4304..>4858)
                     /locus_tag="ACZPDN_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298872.1"
                     /translation="SDSELEFLTAQLDEIENANIQEDELEMLNEQIHEASNWFKNKED
                     LSMCLYEMDKENGALDSLYTLYKQASKSSILNAYEETFKNLYYSLQSVDEELKHIRDT
                     HSNDALDLDSLQNRQYLIKKLYRKYGGSYMSLMESKKNIMDKIDRIIHRQDVFDKLEK
                     EKEESFAAYMKLANELSVKRKDVFS"
BASE COUNT         1592 a          986 c          621 g         1649 t
ORIGIN      
        1 agtcattctc tttagaaaga gtatacttac ttaattgatt cttgatccaa gcggattgtt
       61 cttttacaaa atcatcaatt tctcttgtag aaacatttaa aggagcgctt acacaaacca
      121 ctccttttac tacacgtatt tttattcttt tacaattgga ttttttatga agtatatact
      181 ccattaggca atatcttcta agatttcatt ggcaatatca cttagatatg catctacttt
      241 ttcttgttga tctttagaat atgcatcata ctcattgata tcacgaatca aatccaacat
      301 tggaagtgtt ccatataatt ttgtacaagt tgaattacac aatgtttcca catcttcttt
      361 tttatagaat tcaattcttt catgacaatt tggacattct aagtacgcca tattttcaat
      421 aataccaagc actttcactt ccatcttttc acacatatga caagcctttt ctacaatcat
      481 gctcaccatt ggctgaggtg tagaaaccat cacaacaccc tgcacaggaa ttgattgcat
      541 aattgttagg gcaatatcac cagttccagg aggcatatca attaataaaa catctaaatc
      601 accccataca acatctgtat agaattgttt tacaacacca ccaatgactg gcccacgcca
      661 aatgactgga tctgattcag attgaactaa aaagtttaaa gacatgactt taatgccttc
      721 tttggattcg actggaatca attgttgttt ttcatttcca aacgcatgtt catttgataa
      781 agctaaaagt cttggaatac taggacccgt aatatccgca tccataatac caacttttaa
      841 acctttttta gccatagcct tagctaacat aaccgtaaca gtagatttac caactccacc
      901 cttaccagac attacagtga taatattttt aatgtggtta tctggattgg attgaattcc
      961 acatgtttca ctgctcttac cacattttcc ttgagaagga caaccttcac atgacatata
     1021 ctattcctcc tttgattttt ctattttttg aaaacaacga tcataaacac tcgttaaacg
     1081 cttatcttga atatgcgtat agatttgcgt tgtagaaata ttggaatgcc ccaaaagctc
     1141 ttgaacaatt cttaaatcgg catctccatc taataaatgc gtcgcaaacg aatgcctgaa
     1201 actatgtgca gatatgtgcg gatttaaatt taattcattc actttttgtt taatcatcaa
     1261 gtgaacatat tgccttgtac aaatatggcc taaacgattc acaaaaaaat acgaaacatt
     1321 ctttacatcc caaactaagc gaatggaatc taaataatat ttcatctgtg aaacacaata
     1381 agaagatata ggaatgattc tttccttatc tcctttgcct ttgactttta agatttgctc
     1441 tgtcaaatgc acatcattta gctttaaatc acaaagctca ctaacacgca aaccacaaga
     1501 atataataca actaagattg ttttattata ttggtcgatc tcattgtctt gaaaagatga
     1561 aaaaactttt tttacttcat ctacacttaa ataaacaggc agatgcttag atgatttcat
     1621 cccatgaatt aataaagata catctttgat ttcaggatgc atagttgtta tctgcaaatg
     1681 aaaagaatga atactcgcaa tcatgcgatt cagacttgac gcaacatgat tctcggcata
     1741 aaaatccaaa aacgcttcaa tatctaaaac ggaaatgttt tcacacttag aaatctttcg
     1801 atcctttaaa aaatctaagt aaacctcgat atcatgtaga tacgattgta cagttttctt
     1861 tgattttcgt tgcacaacac tcaaatataa atgataatcc tcgaatgcat cttttaattc
     1921 catgaaacta ttataacaca aaattaaata tatataggtt ctaatacatt catatcgcaa
     1981 ttcgactctt tgattgcctc gatcaataca gattttacct tatgatcacg atgatcatac
     2041 gcaaaccgca aattacgaat actaaagtta ttgtcatata gaactttatt gatctcattt
     2101 aaacgattag gacgatgcac catataaaac cgcccataac ttttcaataa acgattcgca
     2161 tgaaatatca attgttctaa tgttaaattt tcttccattc ttcccaaatg acgaaaattt
     2221 aaatgttccg gtttctgatt ggcattcatt ttaaaataag gaggattgct aacaacgaca
     2281 tcaaataaat catctacata ttcttgaatt ggttgattta caatttttac cggatgctta
     2341 ataaaacaat ccgcattctt ttgagctact ttactcgttt cttctaacac ttcaacaccc
     2401 actagctctt ttatatgaaa ctggtcaaag tacaccaaca aagcaccatt gtttgtacca
     2461 atatcgagaa tacgttcttg tttttttaaa cgaacaaact gcgccaataa tttcgtatcc
     2521 gtattaaagc gaaaatgttc actatcttga tataacttta aatcttcatg aataaaataa
     2581 tccaacgtta aactcaattt gacccctcct tcttcaataa atcacttgcc tttataaaac
     2641 atttactatc cattttcgca tttaaacgat ttaatatgaa atctgtattt tcttgcggta
     2701 cttcaaacaa atccacctgc tctacaattt gtttggcatc ctttaaagaa ccaacatgaa
     2761 caccaatata acgatatcca atcggctcaa aataagcttc taatagctgc atacaagccc
     2821 cataaatcat tggatatgta tttgaatatg tactcaaatt catactgtga cacacattat
     2881 gaaaagcagt atctcttaat gttaaagtga caagtcttcc tttttgattt tcacgctgca
     2941 ttttatgcgt caattcattc acaatgattt gaagattact actcacctca tcaatggaat
     3001 ataaatcatt ggaataggtt ctagagatcg aaatactctt ttgtgttgtt gaataacaca
     3061 aagctgcgct tgaattacca cgagctttct gaatcaaaga ataactcgat ttacctaatt
     3121 tacgtaagat tttttgttcg tttttttcat ccgcaaaatc accaatcgta tagattccca
     3181 tctcatttaa aacgggaaca ctcttttttc caataccatg catctcgttg atttttaatg
     3241 gccacaactt cgttgaaagc tccgattttc taagtactgt gattcctttg ggcttgcgca
     3301 tatcactcgc cattttcgca agaaaacgat tgggtgctac accaatcgaa caattcaaat
     3361 gacatttttc atagactcca ttttgaattt caaacacaag atctaaaggc cttttataac
     3421 gcttgataat atcggtaaca tccatataac actcatcaat acttgctggt tcaatttgat
     3481 gggtaaatgt ctttaaatat gcaaaaaact gtgcggacat cgaacgataa tatccataat
     3541 caccttggat caatactaag tctggacaaa gacgcaaagc ctcataaact ggcatcgcac
     3601 tatgcacacc atattttctt gcttcataat tggcagtcga aatcacgccg cgctttgaag
     3661 tcgatcctac cgcaactgga aattccttta aatccggatt ctttatttct tcacaactcg
     3721 caaaaaaagc atttaaatca atatgtaaaa tcactctagc catgaatctt cacccataga
     3781 tcttgcatag attcttttgc ctttacactc gcttcaccac ttgtcataat agctaactct
     3841 tgaatcgttt ggtcatgatc tagtttttga acacgcgtga ttgtttcatt aaaagtcgat
     3901 tttttactta ctaaataatg cgtgttggcc attacggcaa cacttgctaa atgcgtaata
     3961 cataacactt gatacttatt ggataaagcc ttcattttag cccccattgc caaagcaact
     4021 ttaccactga cacctgtatc aatttcatca aaaataatgg tttcaattcc atttgatgcc
     4081 tgaaatacga cctttaatgc caacatcaaa cgagagagtt caccaccact tgcagataca
     4141 cttaaacttg aaaaatcctg gcctggattc attgacacta aaaattcaat ttcatcaata
     4201 ccatttgaag atggtttctt ttcggtacaa gaaatcttaa aacgagcatt ttctaacatc
     4261 aaatctttac aatgctccat cacctttgat tctnnnnnnn nnnagaaaag acatcttttc
     4321 ttttaacaga caattcattt gccaatttca tataagccgc aaaggattct tctttttctt
     4381 tttccaactt atcaaataca tcctgacgat gaatgattcg atcaatttta tccatgatat
     4441 tctttttaga ttccattaaa gacatgtagg aaccaccata cttacgatac aatttcttga
     4501 ttaaatattg acgattttga agactatcca aatctaaagc gtcatttgaa tgtgtatcac
     4561 gaatatgctt aagttcttca tctacacttt gtaaagaata atataaattt ttaaatgttt
     4621 cttcatatgc atttagtatt gatgattttg aagcttgttt atataacgta tacaaagaat
     4681 ctaacgcacc attttcttta tccatctcgt ataaacacat agaaagatct tctttatttt
     4741 taaaccagtt tgaagcttca tgaatctgtt cgttcaacat ctccaattca tcttcttgta
     4801 tattcgcgtt ttcaatttca tctagctgtg cagttaaaaa ctccaattcc gaatctga
//
LOCUS       DBMDWL010000151         4853 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7274_length_4853_cov_2.748737, whole genome shotgun sequence.
ACCESSION   DBMDWL010000151 DBMDWL010000000
VERSION     DBMDWL010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7274_length_4853_cov_2.748737"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="ACZPDN_09400"
     CDS             complement(<1..410)
                     /locus_tag="ACZPDN_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1298873.1"
                     /translation="MAEPTVLGYIKEQPERLKYVFENKDVFVKPFVDVFTKNNIKKVI
                     FFGSGTSYNVSILGTYYFKHLVGIDAEYQYPTVFKNYEKADWSGLLKKDEILYVGISQ
                     SGTSISTCEVMKYAKQEGYHTLAITGNLNSEITK"
     gene            complement(424..897)
                     /locus_tag="ACZPDN_09405"
     CDS             complement(424..897)
                     /locus_tag="ACZPDN_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HIR1298874.1"
                     /translation="MAIVEVRVDDRLIHGQVCGYWIPQFDVQRILIVDNEIVNDENRK
                     VSLKFGTPPRCKLSIFDSKKAAEKLLRKIDEGIRVMILVRSPEPLAEMLDYGYTFDHV
                     TVGNMSTKDGAKQIRNNMFVDEKEAAAFKKIKEHNIPMYYQITPNVSKDDISKEF"
     gene            complement(907..1743)
                     /locus_tag="ACZPDN_09410"
     CDS             complement(907..1743)
                     /locus_tag="ACZPDN_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR1298875.1"
                     /translation="MAKNDVISVENNLTKKDFRKVFWRSFTLLGSFNYEKMEGLAYLY
                     CIEHILKKIYKDDEEGLKAAMLRESAAFNMTIAPAPFVMGISIAMEESVKRDPNFDPA
                     SINAIKVSLMGPLSGIGDTFFWGIFRILACSLSIGFAKAGNPIAPFILLILFNIPTFL
                     TRYYGLKIGYTSGNQLLIDLEKSGKMQLFTYCAGILGVAAIGCMIASWVTVSCPLEFT
                     ISGNKIVIQEYLDQIMPKLLPLAATLGIYSAIKKNVKITKIISAIVVIGFVLGVFGII
                     AL"
     gene            complement(1747..2532)
                     /locus_tag="ACZPDN_09415"
     CDS             complement(1747..2532)
                     /locus_tag="ACZPDN_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HIR1298876.1"
                     /translation="MTFIQALLIGLIAGLTVLDGNWLGEAKFREPVVTGFLVGLALGD
                     VTKGLKVGAELQLLWMGATGIGPTAQLDIGTGGTIGAAVAIMTGKGAETAILFGVPVA
                     VVMQFINTLLIASYSGLMHKVDNDIEAGNFKSIALTHHFCNWATFALYSLVAFVAMYF
                     GHGVIQAIADGLPAWANAGLNGVAALLPALGFALLLNIIFEKDLTPYLIIGFVLAAYA
                     GQAITMVGITLIAFAIAMIIYQIRSNQGTVVQSASALDDEWED"
     gene            complement(2726..3448)
                     /locus_tag="ACZPDN_09420"
     CDS             complement(2726..3448)
                     /locus_tag="ACZPDN_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1298877.1"
                     /translation="MSEKPIYVQLMDSIKKKIQDGQLQVGDRLVSEREMSEQYGINRM
                     TVRNALKKLQEEGVIETKRGSGNYVAKVPYVEEKLELGRAGVYSLSAQIRQKGMKSSR
                     KTLSLTKIKPEDKLKEVFPNEDYIYEIIRLSYINDEPYALQKVYIPCSKFEDAERFDF
                     ESFSLYDYMDDLGHRPKTMVSYLRIDSLPIEYRKYMDIERNVFIFDYHGYDENRELVE
                     YTISYHNPKYSSFKYVTQRLEV"
     gene            complement(3445..3966)
                     /locus_tag="ACZPDN_09425"
     CDS             complement(3445..3966)
                     /locus_tag="ACZPDN_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HIR1298878.1"
                     /translation="MDKDNIYVLRDIDCFMSVTEAIREQGLTLENRIIEKKVIESNYY
                     LSKQLNVPLATKLFYLCRVRYVEGIAQSIEKTYIEYSKVEGLEEYDLGNTSFYGLLLR
                     EYGIQVYETQEELLLVEAKREEKRLLGLEGKCEILLNRGTSYVSKNDHEPFEYFEMSS
                     LPSFYRYRSVSEI"
     gene            complement(4062..>4853)
                     /locus_tag="ACZPDN_09430"
     CDS             complement(4062..>4853)
                     /locus_tag="ACZPDN_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298879.1"
                     /translation="HFRQLLDLYEKGELTNVINRVVEDMNHRFTKSVVERITANNLLR
                     DRVDSLNLDKVTLGIKNMLDILNKEEQSIDISDVHMTQIKKYLSILDLIKEIDLEYLP
                     EVNQKSKIIVVTQPGLRYAQAEAIVENLLLDEKFQELDIQKRTRILERLLSEIRGRMM
                     EDIVLLETKLAKKDKHVFKLQFPIGEFDMVVQDPKTLSCEIYEIKYSKERACEQYRHI
                     NDEEKCAMTTHRYGNITGRYVIYRGENTEMDTIQYLNVEDYLKSL"
BASE COUNT         1558 a          986 c          621 g         1688 t
ORIGIN      
        1 tatttagtga tttcactatt tagattccca gtgattgcca aagtatgata accttcttgt
       61 tttgcgtatt tcataacttc acaagtcgaa atggatgttc cagattgaga aataccaaca
      121 tatagaattt catccttttt taataatcct gaccaatctg ctttttcata atttttaaat
      181 acagttggat attgatattc tgcatcaatt cctactaggt gtttgaaata ataagttcct
      241 aaaatagata cattataaga tgttcctgaa ccaaagaaaa tcactttctt aatattattt
      301 tttgtgaata catctacaaa tggtttcaca aaaacatctt tattttcaaa aacatatttt
      361 aatctttctg gttgttcttt aatatatcct aatactgttg gctctgccat aatcttatcc
      421 tccttaaaat tcttttgaaa tgtcatcttt agatacattt ggtgtaattt gataatacat
      481 tggaatattg tgttctttga ttttcttgaa agcagctgcc tctttttcat ctacgaacat
      541 attgttacga atctgtttag cgccatcttt tgtagacata ttacccacag taacatgatc
      601 aaatgtatat ccataatcta acatttcagc aagtggttct ggtgaacgaa ctagaatcat
      661 aacgcgaatg ccttcatcaa ttttacgaag caatttttcg gctgcttttt tcgaatcaaa
      721 aattgacaat ttacatcttg gtggagtacc aaatttcaaa gatactttac gattttcatc
      781 attcacgatt tcattatcga caatcaaaat tcgttgtaca tcaaactgtg gaatccaata
      841 gccacaaact tggccgtgaa tcaaacgatc gtccacacgt acttctacta tagccataat
      901 aacctcctat agggcaataa tgccaaatac acctaacaca aatccaatca ctacaatggc
      961 tgaaataatt tttgtgattt taacattctt tttaattgct gaataaatac ctaatgttgc
     1021 agctaaaggc aataatttag gcataatttg atctaaatat tcttgaatta caattttgtt
     1081 tcctgaaatt gtgaattcta aaggacagga tacagttacc cagcttgcga tcatacaacc
     1141 aatggcagcc acaccaagaa tacccgcaca atatgtaaac aattgcattt ttcctgattt
     1201 ttctaagtca atcaacaatt ggtttccact tgtatatcca atctttaatc cataataacg
     1261 agttaagaat gttggaatat taaatagaat caatagaata aatggagcaa ttggatttcc
     1321 tgctttcgca aaaccaatag ataatgaaca agccaagatt ctaaagatac cccagaagaa
     1381 tgtatctcca ataccactta atggacccat caaactgact ttgatcgcat tgattgacgc
     1441 tggatcaaaa ttaggatcac gtttaacgct ttcttccatc gcaatagaaa ttcccataac
     1501 gaatggtgct ggtgcaattg tcatattgaa agctgcactt tcacgtagca ttgctgcctt
     1561 taagccttct tcatcatctt tataaatttt ctttaaaata tgttcaatac aatatagata
     1621 agcaagacct tccatctttt catagttgaa tgatccaagt aaagtaaatg atctccagaa
     1681 tacttttcta aaatcttttt ttgttaaatt attttcaaca ctaattacat catttttagc
     1741 catgttctag tcctcccatt catcatccaa agctgaagca ctttgaacaa ctgttccttg
     1801 gtttgagcga atttgataaa taatcattgc gattgcgaat gcgatcaatg tgataccaac
     1861 catagtgatt gcttgacctg catatgctgc aagaacaaat ccaataatta agtatggtgt
     1921 taaatctttt tcgaagataa tgttcaacaa taaggcaaat cctaatgcag gaagtaaagc
     1981 agctacaccg ttcaatccag catttgccca tgcaggaagt ccatctgcga ttgcttgaat
     2041 tacaccatgt ccaaaataca tagctacaaa cgcaactaat gaatataatg cgaatgttgc
     2101 ccagttacag aaatgatgag tcaatgcgat tgacttaaag ttacctgctt caatatcatt
     2161 atctactttg tgcatcaatc cagaataaga agcaattagt aatgtgttaa taaactgcat
     2221 aacaacggcg actggaacac caaatagaat cgctgtttca gcacctttgc cagtcataat
     2281 cgcaacagct gcaccaattg ttccaccagt accgatatca agttgagctg taggaccaat
     2341 accagttgcg cccatccata aaagttgtaa ttcagcacca accttcaatc ctttcgtaac
     2401 atctcctaaa gctaaaccaa ctaagaatcc tgtaacaaca ggttcacgaa atttagcttc
     2461 acctaaccag tttccatcca aaacggttag accagcaatc aagccgatta gtaatgcctg
     2521 aataaatgtc atatttctct cctccttaat gacattggta tacacttaat accatttttt
     2581 gattaattta taccatacat gtataccaat gtcaataatt tctttggcac acaggcatac
     2641 caattctaga tatgcctaca aaaaaagaga tatcctcaat ttgatacctc catttcgata
     2701 tacttatata ccaatttatt gacttctaaa cttcaagcct ttgggttaca tatttaaacg
     2761 atgaatactt tggattatga tatgaaattg tatactccac caattcacga ttctcatcat
     2821 agccatggta atcaaaaata aatacatttc tttcaatatc catatatttt cgatactcta
     2881 ttggcaaaga atcaatgcgc aaataagaaa ccatagtttt aggacgatgt cctaaatcat
     2941 ccatatagtc atataatgaa aaactttcaa aatcaaaacg ctctgcatcc tcgaatttag
     3001 aacaaggaat atataccttc tgcaatgcat aaggctcatc attgatataa gataaacgaa
     3061 tgatttcata tatataatct tcatttggaa agacttcctt taatttatct tcaggcttta
     3121 tctttgtcaa agatagtgtt ttacgactcg atttcattcc cttttgacga atctgcgcac
     3181 tcaatgaata aacaccggca cgacctaatt ctagtttttc ttcaacatat ggaactttcg
     3241 caacgtagtt gccacttcct ctttttgttt caattacccc ttcttcttgt agttttttta
     3301 atgcatttcg caccgtcatt cgattgattc catattgttc ggacatctct ctttcactca
     3361 ctaaacgatc tccgacttga agctgtccat cctgaatttt ctttttgatg ctgtccatca
     3421 gttgtacata gattggtttt tcactcatat ttctgacaca ctcctatatc tataaaagct
     3481 tggtagactt gacatttcaa aatattcaaa aggctcatga tcattttttg aaacataaga
     3541 agttccacga tttaataaaa tctcgcattt tccttctaat cctaataatc ttttttcttc
     3601 cctttttgcc tccaccaata ataattcttc ttgtgtttca taaacctgta taccatattc
     3661 ccttaataaa agcccataaa acgatgtatt cccaagatca tattcttcaa gtccttccac
     3721 cttcgaatat tcaatatacg ttttttcaat ggattgagcg atgccttcta catatcgaac
     3781 tcgacataaa tagaagagtt tagtagctaa tggcacattt aattgttttg acaaataata
     3841 attggattca atcactttct tttcaataat acgattttct aaagttaaac cttgttcacg
     3901 aatcgcttct gtaaccgaca taaaacaatc tatgtcgcga agtacatata tattatcctt
     3961 atccatactc ttttctccct atataattgg tatacccatc atatcatgtt ttggtataca
     4021 aaaaaaggag acgattcata aatatcgcct caatttgatc cctacaaact ttttaaataa
     4081 tcttcaacgt ttaaatactg aatcgtatcc atctctgtat tttctccacg ataaatcaca
     4141 taccttccgg taatattgcc atatcgatgt gtcgtcatcg cacacttttc ttcatcattt
     4201 atatgtcgat attgttcaca tgcccgttcc ttactgtatt ttatttcata tatttcacat
     4261 gataatgttt tcggatcttg cacaaccata tcaaactcac caattggaaa ttgcaattta
     4321 aagacatgtt tatctttttt tgcgagtttt gtttccaaaa gaactatatc ctccatcatt
     4381 ctgccacgaa tttcactaag taaacgttct aaaatacgtg tccttttttg gatatctaac
     4441 tcttggaatt tttcatctaa aagaagattt tcaacaatag cttcggcttg tgcatatctt
     4501 aaaccaggct gtgtaaccac tataatttta ctcttctggt ttacttctgg taaatattct
     4561 aaatcaattt cttttattaa atccagtatt gacagatact ttttaatttg agtcatatgc
     4621 acatcactaa tatcaatact ttgttcttcc ttatttaaaa tatcaagcat attttttatt
     4681 cccagagtca ccttatctag atttaatgag tccactctat ccctcaataa attattggca
     4741 gtgattcttt ccacaacact ttttgtaaat ctatgattca tatcttctac tactcgatta
     4801 atcacattcg taagctctcc tttttcatat aaatctaaaa gctgacgaaa atg
//
LOCUS       DBMDWL010000152         4806 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7377_length_4806_cov_2.107545, whole genome shotgun sequence.
ACCESSION   DBMDWL010000152 DBMDWL010000000
VERSION     DBMDWL010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7377_length_4806_cov_2.107545"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            364..2271
                     /locus_tag="ACZPDN_09435"
     CDS             364..2271
                     /locus_tag="ACZPDN_09435"
                     /inference="COORDINATES: protein motif:HMM:NF014740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR1298880.1"
                     /translation="MSKNRKENNTRWSLVVYDVIVYAISSLLLIWLYGGNESLTNTES
                     LKLTVLAFAIIFVCRLIGHIYGQIWRYGGIQGYIRMIVSDGVAFILLYVVQMAMPIQR
                     MSFARTLSLVCVNLLGSITIRMIYRYCYLYSDNVTKKGKFLAKLLYRFSSIETDTNKE
                     VQKIKIAIIGAGRVGASFAEDLLNDENAVYTPRCFIDTNKEKAGREVHGIPVWYADET
                     TLDKLKAYEVQEIVFAIPSMDVNKKKCLYEHYKNAGYKVKVYDYPTLYTAGSKRNLRE
                     FDIEELLFRKPLAVTNETTNAYYKDKVILITGGGGSIGSELCRQLAKMEPKLIVILDI
                     YENGAYDVQQELKIAYGNDLNLQIEICSITYKKALEKVFKKYHPQIVINAAAHKHVPL
                     MEHNCVEAIYNNVFGTKNLIELCEKYEAQRFMMVSTDKAVNPTNVMGATKRMCEMMVQ
                     SASTYGHVKYSATRFGNVLGSAGSVIPLFKRQIAKGGPVTLTDKRIIRYFMTIPEASQ
                     LVLQSGAMANNGELFVLDMGQPVKILDLAQNMIKLSGAHDIEIVETGLRPGEKLYEEL
                     LINSQTLTKTDNDLIFIEKDTPLSKEEIDEKLEVLKEAIESEDDNDAREALRSVVPTF
                     RRPEEINKQVA"
     gene            2289..2999
                     /locus_tag="ACZPDN_09440"
     CDS             2289..2999
                     /locus_tag="ACZPDN_09440"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR1298881.1"
                     /translation="MMNIPFSPPDISEEEVKEVTEALKSGWITTGPKTKQLEKEVADL
                     CGVNKAVCLNSQTACAEMTLRLLGVGPGDEVITSAYTYTASASVVCHVGAKLVLIDTQ
                     EDSLEMDYEKLENAITEKTKVIIPVDLGGVPCDYDRIFSIVEKKKDLFNPTNDIQKAI
                     GRCIIMTDAAHAFGATWHNKPVGSIADFSNFSFHAVKNFTTAEGGAVTWKDIDGIDNE
                     VIYKKYQLLSLHDNLKMP"
     gene            3059..3496
                     /locus_tag="ACZPDN_09445"
     CDS             3059..3496
                     /locus_tag="ACZPDN_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013227.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR1298882.1"
                     /translation="MTDVVAGIGLGQMRRYKGMLERRKEIIQKYDVAFKPLGVEVLNH
                     YTDEHQSSGHLYITRIPNITLEQRNEIIIKMAEQGIATNVHYKPLPMMTAYKNLGFDI
                     KDYPNAFKQFENEITLPLYTKLTDEEVDFIIENYCNMINKVKN"
     gene            3496..3957
                     /locus_tag="ACZPDN_09450"
     CDS             3496..3957
                     /locus_tag="ACZPDN_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014453.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR1298883.1"
                     /translation="MLKNWNELPEYMRTDEVRPYYDLLQRKKLSLFFKRVFDIVVSLI
                     MIILCSPILLIISILIVKDSKGGVFYRQERVTQYGRVFRIFKFRTMVQNADQIGTQVT
                     VSNDSRITKIGSKLRNCRLDELPQLFNIFLGDSGIIGTTKKNLDFTRVSLA"
     gene            4192..4704
                     /locus_tag="ACZPDN_09455"
     CDS             4192..4704
                     /locus_tag="ACZPDN_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR1298884.1"
                     /translation="MGRKLLIVASKRYGDYVKEIAESMGCFEEISFVDNDREGAIGKL
                     EEVETFYPEYNCAIAACDDGAKRLEWNKKLEALYNIPVLAHMDSTISPSANLMPGCIV
                     EPKAVVGCGSTIGQATVVGANTVVEPYSFIGDSCTLKSGTIVKSTSVLQIGTETEQGT
                     VLFTPLTSKQ"
BASE COUNT         1721 a          599 c          979 g         1507 t
ORIGIN      
        1 ggaaattttc taaaaaatat ttttaatttg ttatatgatg cttatgcgtt ttaaataagg
       61 gtaatattaa atatatttaa tgttttcacc taagcttaag taacaaatgt tcattgacgg
      121 ggggggtatt aggctataat atataagcaa ctgttattct tgggaagggt acggtttatt
      181 tttagaatca gtgtttatag ggtacataca taggatatta aaaatagctg gttttccgca
      241 tttttaattg aattgttcat ctttttattt gctttagata gggagaagtg tatctattta
      301 tagaatgatt atgtattgac tcgtgttttt tagcgagttt tttttatgga atgggagatt
      361 tggatgagta aaaatagaaa agaaaataat acgcgttggt ctttggttgt gtatgatgtg
      421 attgtttatg cgatatcttc cttattatta atatggttgt atggtggaaa cgaatcatta
      481 acaaacacag aatctttaaa acttacagta ctggcatttg cgattatctt tgtatgtcgt
      541 ttaattggtc atatttatgg tcagatttgg cgttatggcg gtattcaagg ctatattcgt
      601 atgattgttt cagatggtgt agcatttata cttctatatg tagttcaaat ggcaatgcca
      661 attcaacgaa tgagttttgc aagaactcta agtttagtat gtgtaaactt attgggttcc
      721 attactattc gtatgattta tagatattgt tatttatata gtgataatgt aacgaaaaag
      781 ggtaagtttc tagctaagtt actatataga ttttcaagta ttgaaacgga tacgaataaa
      841 gaagttcaaa agatcaagat tgcgattatt ggtgcaggcc gtgtcggtgc tagttttgca
      901 gaagatttat tgaatgatga aaatgcagta tatacaccac gttgttttat tgatacgaat
      961 aaagaaaaag cgggtagaga agtgcatggc attccagtat ggtatgcgga tgaaacgact
     1021 ttagataaat taaaggcgta tgaagtacaa gaaattgtat ttgcgatccc atctatggat
     1081 gtaaacaaga agaaatgttt atatgaacat tataaaaatg ctggatataa agtaaaggta
     1141 tatgactatc caacactata tactgcagga agtaagcgta acttaagaga atttgatatt
     1201 gaagaattat tatttagaaa accattagct gtcacaaacg aaactaccaa tgcttattat
     1261 aaagataagg taattttaat tactggtggt ggaggatcca ttggatctga actttgtaga
     1321 caacttgcta agatggaacc aaaattgatt gttatcttag atatttatga aaatggtgct
     1381 tatgatgtac aacaagaatt aaagattgcc tatggcaatg atttaaatct acagattgaa
     1441 atttgttcaa ttacatataa gaaagcatta gaaaaagtat ttaagaaata tcatcctcaa
     1501 attgtgatta atgcagctgc acataaacat gttcctctaa tggaacacaa ttgtgtggag
     1561 gctatttata acaatgtatt tggaacaaag aatttaattg aactttgtga aaaatatgaa
     1621 gcacaacgtt ttatgatggt atctactgat aaagcagtaa acccaaccaa tgttatggga
     1681 gccactaagc gtatgtgtga aatgatggtt caatccgcaa gtacttatgg acatgttaaa
     1741 tatagtgcaa caagatttgg aaatgtttta ggaagtgcag gaagtgtaat tccattattt
     1801 aaaagacaaa tcgctaaagg tggtcctgta acattaacag ataaacgaat tattcgttac
     1861 tttatgacta ttccagaagc aagtcaattg gtattacaat caggggctat ggcaaataat
     1921 ggagaattat ttgtactaga catgggtcaa ccagtaaaga ttttagattt agcacaaaac
     1981 atgatcaagc tttctggagc acatgatatt gaaattgtag aaacaggatt gcgtcctgga
     2041 gaaaaattat atgaagaact attaattaat agtcaaacat taacaaaaac agataacgac
     2101 ttgatcttca ttgaaaagga tacaccacta tctaaagaag aaattgacga aaaacttgaa
     2161 gtattaaaag aagcaattga atctgaagat gacaatgatg ctagagaagc attgagaagt
     2221 gttgtaccaa cattcagaag accagaagaa ataaataagc aagtcgctta ggggaggata
     2281 aagagtttat gatgaatata ccattttcac caccagatat ttcagaagaa gaagttaaag
     2341 aggttactga agccttaaaa tctggttgga ttactacagg gccaaaaaca aagcagttag
     2401 aaaaagaagt agctgatctt tgtggagtaa ataaagcggt atgtcttaat tcacaaacag
     2461 catgtgctga aatgacattg agattattgg gggtaggacc tggtgatgaa gtgattacca
     2521 gtgcgtatac atacacggca agtgcttctg ttgtatgtca tgttggagca aaattagttc
     2581 taattgatac acaagaagac tcattagaaa tggattatga aaaattagaa aatgcgatta
     2641 ctgaaaagac aaaggtaatc attcctgtgg atcttggggg agttccatgt gactatgata
     2701 gaattttctc tattgtagaa aagaaaaagg atttgtttaa tcctacaaat gacattcaaa
     2761 aagcaattgg aagatgcatt attatgacag atgctgctca tgcatttggc gctacttggc
     2821 acaataaacc agtaggcagt attgcagact tctctaactt tagtttccat gcagtaaaaa
     2881 actttactac tgctgaaggt ggagctgtta cttggaaaga tattgatggt attgataatg
     2941 aagtgattta taaaaaatat caattattat cattacacga caatctaaag atgccttagc
     3001 taagactcaa ttaggagctt gggaatatga tatcgttggt ccttggtata aatgtaacat
     3061 gacagatgtt gttgcaggta ttggcttagg acaaatgaga agatataagg gaatgttaga
     3121 aagaagaaaa gagatcatcc aaaagtatga tgtagcattc aaaccattgg gtgtagaagt
     3181 attaaatcat tatacggatg aacatcaatc ttctgggcac ttgtatatta caagaattcc
     3241 taatattaca ttggaacagc gtaatgaaat cattattaaa atggcagagc aaggtattgc
     3301 cacaaatgtt cattacaagc cacttccaat gatgactgcc tacaaaaact taggatttga
     3361 tatcaaagat tatcctaatg catttaagca atttgaaaat gaaatcactc ttcctttgta
     3421 tacgaagtta acagatgaag aagtggattt tattattgaa aactattgta atatgattaa
     3481 taaggtgaaa aattaatgct taagaattgg aatgaattgc ctgagtatat gcgaacagac
     3541 gaagttcgtc cttattatga tttattacaa agaaaaaaat taagtttatt ttttaagaga
     3601 gtatttgata ttgtagtttc acttatcatg attattcttt gttcaccaat tctattgatt
     3661 atttcaatat tgattgtgaa agattctaag ggtggcgttt tctatagaca agaaagagtt
     3721 actcaatatg gtagagtatt tagaatcttt aagtttagaa caatggtaca aaatgcagat
     3781 caaattggaa cacaggtaac cgtatcaaat gattcaagaa ttacaaagat tggaagtaaa
     3841 cttagaaatt gtagattaga tgaattacca caattgttca atatcttctt aggagatagc
     3901 ggtataatag gaacaaccaa gaaaaacctt gattttacaa gggtttcgct ggcttagtcg
     3961 aattttgtta tgataatttt tctgtatggt tgtgtcgagt aagttacgaa actgcgaaag
     4021 tgaatggttt cttggatgct tattttgacc cacactataa gctgaaaaat tatgataagg
     4081 aggtttgacc tagcctgcga atgatctttt taataagaac attcacaggc tagtcgtcgt
     4141 gttgaagaac ttacctaata aaaataaata attatgagga gtgtattgaa aatgggaaga
     4201 aaattattaa ttgtagcctc taaaagatat ggagattatg tgaaagagat agcagagtca
     4261 atgggttgct ttgaagaaat ctcctttgta gataatgaca gagaaggtgc tattggaaaa
     4321 ctggaagaag tagaaacttt ttatccagaa tataactgtg caatagctgc atgtgatgac
     4381 ggtgcaaaaa gacttgaatg gaacaagaaa ttagaagcac tttataatat tccggtttta
     4441 gcccatatgg attctacaat ttctccatca gctaacttaa tgcctggttg cattgttgag
     4501 ccgaaagcag ttgttggttg tggaagcact atcggtcagg caacagttgt tggtgctaat
     4561 acagtagtgg agccatatag ctttattgga gatagctgta cattaaaatc tggaacgatt
     4621 gtgaaatcaa caagcgtact tcaaattgga actgaaacag agcagggaac tgttttgttc
     4681 acgccactta caagtaaaca gtaaacagtg aaaaaataaa aaagtgaaag gatgaaaacc
     4741 tccccgattt tcgtgggggg ggggtacaca atgaatagtc aatcaaatga aaaaaatatg
     4801 gatatg
//
LOCUS       DBMDWL010000153         4801 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7390_length_4801_cov_2.571277, whole genome shotgun sequence.
ACCESSION   DBMDWL010000153 DBMDWL010000000
VERSION     DBMDWL010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_4801_cov_2.571277"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="ACZPDN_09460"
     CDS             complement(<1..912)
                     /locus_tag="ACZPDN_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR1298885.1"
                     /translation="MNQLTSKQQIYNYLQKMSQHFQLDRFSNFTTITISKELSISRSL
                     TSQYLNELVKDDLVVKISSRPVYYLSKNALEQIYHVVLKQNEYISIHELMQELKYSSP
                     DLKDFQKSVGYDGSLSYPISQIKSALLYPDGLPIILYGERGTGKMYLVSCMKEFCQNH
                     LNEKGHIVLDVKKVSLHEERTAQMEDLFGYEHNGEVKKGILENSDGKMLCIQDAQYLS
                     DQAQEKIADFLRYGYFCREGNEYVRVQSKVRLVLCIENEPRISLNENLLINIPVICHI
                     PSLQERSEDERTRLVVHFFQEEQERLKR"
     gene            complement(1201..2670)
                     /gene="malQ"
                     /locus_tag="ACZPDN_09465"
     CDS             complement(1201..2670)
                     /gene="malQ"
                     /locus_tag="ACZPDN_09465"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1298886.1"
                     /translation="MNRSAGILCPVFSIPANQGIGDLGQKTIRMIDIIADAGYSIWQI
                     LPLQMTGPTHSPYQTYSSFAGDPIYINLDRLCEMGLLTQSSIVNCNKFKDFIEYDKIR
                     EFKEPYFQKAFKVFKKEFDSFKEDFEAFKRDTFWLEEWSAYSLFKSFYNGAPWFEWED
                     EYKNWPKNRDIDLNDYQNEILYIQFLQFIFYKQLDEIQMYAHARNLKIMGDMPFYVDL
                     DSADVWCNREAFLLDEEGKPEFIAGCPPDYFSETGQRWGMPIYDFDAQEENGYLFWCN
                     RMSWMNRCYDMVRIDHFRAFDTYWKIPESCPTAIEGKWILGPAHKLMDAILKACPDIV
                     LVAEDLGLIRPEVIELEDDYGIPGMDVLLFRMETKQLKKKAKKNSVLYTGTHDNATCN
                     EDYHTYDSNKRISLRRFFKNQGYSSRSFNEMLCQYALDTDADTVILPIWDICGFKEEA
                     RINTPGTVSNKNWTWKLKDFKTFPDKLMSTKEWIKKSNR"
     gene            complement(2757..>4801)
                     /locus_tag="ACZPDN_09470"
     CDS             complement(2757..>4801)
                     /locus_tag="ACZPDN_09470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1298887.1"
                     /translation="NIRVYSDLENANMVVWGTYLENNQKKSFFTNTNSNQIASVEAII
                     SHYAVKIEDNKAIFKGRSDALYNFLDYGIPLIQKQADVYVSEELKRLKNRRSMNLSVK
                     VYVQNDLLKMELDSDINADELSHILNAYRKKKKFYQLKNGEMIDLEDTGLEQLDELAS
                     TMNLTAKDFKKETIEKPAYQAFHLMDVDSELDVRNDDSVTEYTNRLMKVKEQTIQLKD
                     EYKSLLRTYQQQGIQWLYDLKNMGLNGILADDMGLGKTLQVLVFYEQYVSKDKPSLIV
                     CPSSLMYNWMSEIEKFKIDVNAVCVTGMQEARKDIIKENYELYITTYDYLRRDVELYA
                     PMEFEYIILDEAQFIKNPKTKNAQSVKSLKSRHRLALTGTPIENGLSELWSIFDFLLP
                     GYLYSLNYFTKNFEKPIQMGDEKRKSQLQKLVSPFILRRTKKQVLKDLPDKVEKDMWL
                     NFSPEEKQLYLANLAQVNEQLQQQLELEKVDSILILAMMTRLRQICCEPRMLYENYKG
                     ESTKFSMCLDLIETLKENGKKVLLFSSFTSIFDDFIEEFDRRGIKYHMITGAVDKKKR
                     KEEVDAFQNDDSNVFLISLKAGGTGLNLTKAQAVIHFDPWWNISAQNQATDRAYRIGQ
                     TKNVLVYQLLMKNTIEEKIYEMQKRKKEMSDLFVENSNGGISTLSKEELKDLFSMD"
BASE COUNT         1524 a         1006 c          575 g         1696 t
ORIGIN      
        1 tctttttaac cgctcttgtt cctcttggaa aaaatgaaca actaaccgtg tacgctcatc
       61 ttcactacgt tcttgtaaac taggaatatg acagatcact ggtatattga ttaacaaatt
      121 ttcatttaat gaaattcttg gttcattttc aatacaaaga acaagcctta cttttgattg
      181 aacacgaaca tattcattgc cctctcgaca aaagtatcca taacgaagga agtctgcaat
      241 tttttcttga gcttgatccg acaaatattg tgcatcttgt atacacaaca ttttcccatc
      301 tgaattttcc aagattccct tctttacttc accattatgt tcatatccaa acagatcttc
      361 catttgagct gttcgctctt catgtaaaga aacctttttt acatctaaaa caatatgacc
      421 tttttcattc aaatgattct ggcaaaactc tttcatgcag cttaccaagt acatctttcc
      481 tgttccacgc tctccatata gaataattgg aagtccatct ggatataata atgcagattt
      541 tatctgagaa atcggataac tcaagctacc atcatacccg actgattttt ggaaatcctt
      601 caaatctgga gaactatatt ttaattcctg cattaattca tgaattgaaa tatattcatt
      661 ttgttttaga acaacatgat aaatttgttc taaagcattt ttcgacaaat aataaacagg
      721 tcttgaactg attttcacaa ccaaatcatc ttttactaac tcatttaaat attgagaagt
      781 taaagaacgc gaaatactta attctttact aattgttatt gttgtgaaat ttgaaaaacg
      841 atctagttga aaatgttgcg acattttttg aagatagtta taaatctgtt gttttgacgt
      901 taactgattc attttacacc tcctaacaaa tatcgcctgt cgacgatatc attttattaa
      961 gaaatcaatt tttagtctgt agttccaaat aaaaaagaca ctattcggta aattttaagt
     1021 aaaatcgaca ctttcgacat ctactttcaa ttactgttaa gccctttcat ttttaattgt
     1081 atcagcattc gacactaaat tgcaaaaatt ctctattgtg tctaattgca cgacatttac
     1141 ttttttacga tagaaaaaga gcaaaaattg atgatttcta ctctataatt cactttctct
     1201 ttaacgattt gactttttga tccactcttt tgtactcata agcttatctg ggaatgtttt
     1261 aaaatctttt aatttccaag tccagttttt attcgacaca gttcctggtg tattgattcg
     1321 tgcctcttct ttaaatccac aaatatccca aatcggcaat atgacagtat ctgcatccgt
     1381 gtctagagca tattgacaaa gcatctcatt aaaggaacga cttgaatatc cctgattttt
     1441 aaagaatcga cgcaaactaa tacgcttatt tgaatcatat gtatgataat cttcattaca
     1501 tgtcgcatta tcatgtgtac ctgtatataa cacagaattt ttctttgcct ttttctttaa
     1561 ttgcttagtt tccattctaa acaataaaac atccatgcca ggtataccat aatcatcttc
     1621 aagttcaatc acttctggac gaatcaatcc taaatcttca gccaccaaaa caatatctgg
     1681 acacgctttt aaaatcgcgt ccatcaattt atgagctggt cctaagatcc attttccttc
     1741 aatagctgtt ggacaagatt ctggtatctt ccaatatgta tcaaaagctc taaaatgatc
     1801 aattcttacc atgtcataac aacggttcat ccaactcata cgattgcacc aaaaaagata
     1861 tccattctct tcttgcgcat caaaatcata gattggcata ccccaacgtt gtcctgtttc
     1921 actaaaataa tccggaggac agccagcaat aaattctggt tttccttctt catctaataa
     1981 gaaagcctca cgattgcacc acacatctgc actatctaaa tctacataga aaggcatatc
     2041 ccccataatt ttcagattcc ttgcatgtgc atacatctgg atctcatcta attgtttata
     2101 aaatataaac tgtaaaaact gaatatacaa aatttcattc tgatagtcat ttaaatcgat
     2161 atcacgattt tttggccaat ttttatattc atcttcccat tcaaaccatg gagccccatt
     2221 gtaaaaactc ttaaacaacg agtaagcaga ccattcttct aaccagaatg tatcgcgttt
     2281 aaacgcttca aaatcttctt tgaatgaatc aaattctttt ttaaaaactt taaaggcctt
     2341 ttgaaaataa ggttccttaa attcacgaat cttatcatat tcaataaagt ctttaaactt
     2401 attacaattc acaattgaac tttgcgtcaa taatcccatt tcacacaaac gatccaagtt
     2461 aatataaata ggatctcccg caaaactaga ataagtttga tatggagaat gagtgggacc
     2521 agtcatttgt agcggcaata tttgccatat actgtaaccc gcatccgcaa taatatcaat
     2581 cattcgaatt gttttttgcc ctaaatcacc aatcccttga tttgcaggga tagagaaaac
     2641 aggacataaa attcctgcac ttctattcat tttcgcgcct tatgtaatcc ctttcattac
     2701 aactatttta tcacaattca taagaaaaag gcaatatatt tcaaaaatat attgccttaa
     2761 tccattgaaa acaaatcttt tagctcttcc ttagataatg ttgatattcc accattcgaa
     2821 ttttcaacaa ataaatcact catttctttt ttgcgctttt gcatttcata aatcttttcc
     2881 tcaatcgtat tcttcatcaa caactgataa acaagaacat tctttgtctg tccaatacga
     2941 tatgcacggt ctgtcgcttg attttgtgca cttatattcc accaaggatc aaaatgaata
     3001 acggcctgtg cctttgttag attcaaacct gttccgcctg cctttaaaga aatcaagaat
     3061 acattggaat catcattttg aaaagcatct acttcttctt tacgcttctt tttatctacc
     3121 gcacccgtaa tcatatgata tttgatgcct cgtcgatcaa attcttcaat gaaatcatca
     3181 aaaatacttg taaaactaga aaacaacaaa accttcttgc cattctcttt taaggtttca
     3241 atcaaatcca aacacatcga aaattttgta ctttcaccct tatagttttc gtataacatt
     3301 cttggttcgc aacaaatctg acgtaaacgc gtcatcatcg caagaatcaa aattgaatct
     3361 accttttcta gttctagttg ctgttgaagc tgctcattga cctgagccaa attcgcaaga
     3421 tacaattgtt tttcttccgg actaaaattc aaccacatat ccttctcaac tttatccggc
     3481 aaatctttta gtacttgttt ttttgttctt cgcaaaataa atggagaaac caacttttgt
     3541 aactgcgact tccttttttc atctcccatt tgaattggtt tttcaaaatt ctttgtgaaa
     3601 taattcaaac tatataaata tccaggcaat agaaaatcaa aaatactcca caattcactt
     3661 aaaccattct caattggcgt acccgttaaa gctaaacgat gtctactctt taatgatttt
     3721 acactttgcg cattcttcgt ttttgggttc tttataaact gcgcctcatc caaaataata
     3781 tattcaaatt ccattggcgc atataattcc acatctcttc gcaagtagtc gtatgttgtg
     3841 atataaagct catagttttc tttaataata tctttacgag cttcttgcat ccccgtaaca
     3901 catacggcat tcacatcgat tttaaatttt tcgatttctg acatccagtt atacattaaa
     3961 ctacttggac atacaatcaa gcttggttta tcttttgaaa catactgctc ataaaatacc
     4021 aagacttgta atgtttttcc aagtcccata tcatcggcaa gaattccatt taatcccata
     4081 ttttttaaat catacaacca ttgaatgccc tgctgctgat atgtgcgcaa taaagattta
     4141 tattcatcct tcaattgaat cgtctgttct tttactttca ttaatcgatt tgtatattct
     4201 gtaaccgaat catcattacg aacatccaat tctgaatcta cgtccattaa atggaacgct
     4261 tgatacgcag gcttttcaat tgtctctttt ttgaaatctt tcgctgttaa attcattgtc
     4321 gaagccaatt catccaactg ttctaatcct gtatcttcca aatcaatcat ttcaccattc
     4381 tttaattgat aaaacttttt cttctttcga tatgcattta aaatatgcga caattcatcc
     4441 gcattgatat cactatccaa ttccatcttc aataaatcat tttgcacata gactttcaca
     4501 ctcaaattca tactgcgacg atttttcaat cgtttcaact cttccgaaac atacacatcc
     4561 gcctgttttt gaatcaatgg aataccataa tccaaaaaat tatagagtgc atcacttcta
     4621 cctttaaaaa tagccttgtt atcttcaatc tttaccgcat aatgcgaaat aatagcttca
     4681 acactcgcta tttgattgga atttgtattc gtaaagaaac tcttcttctg gttattctct
     4741 aaatatgttc cccatacgac catattggca ttttctaaat ccgaatacac acgaatattc
     4801 t
//
LOCUS       DBMDWL010000154         4771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7458_length_4771_cov_1.949679, whole genome shotgun sequence.
ACCESSION   DBMDWL010000154 DBMDWL010000000
VERSION     DBMDWL010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7458_length_4771_cov_1.949679"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="ACZPDN_09475"
     CDS             <1..1260
                     /locus_tag="ACZPDN_09475"
                     /inference="COORDINATES: protein motif:HMM:NF021436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HIR1298888.1"
                     /translation="WSHYLTAYSQSSVLLHKKAFDEHGNVILYEDGSIYFVREHSPTR
                     STYAIEYQELKGQQLVSYKGDTITRGPLKLATFAYVPQTFGMTLARLMHLNSTQVAFV
                     GRIFALLFYAFLISQGIKLAPEFAKRILLVVSLLPMVMQQVCSYNYDSILFGICFFSI
                     GYLLNLIHREEKINKKDFIILALCATVIASIKFIYLPVFGLGLLIPKERFRIKRGKEL
                     TILGILCICAFAFIGVTLFNKRFWDLHPTNPGLPNEYMTYSISYLFTHVKDELVLLSR
                     TVQNYTADYISQMISSPLGWVEISMPATQLMGFGCMLALSCMGTEKDKKYSLIENLWL
                     VVLFTGVAFTVLIALQTSYTPTFLDMVYGVQGRYFLPILPLLMIAVRGLFVLKEDSTK
                     IENSLIVGNIFFHITEVSTILFIVIGR"
     gene            1332..2048
                     /locus_tag="ACZPDN_09480"
     CDS             1332..2048
                     /locus_tag="ACZPDN_09480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1298889.1"
                     /translation="MEEKVLIIIPAYNEEESILNTYKTILEYNQTHTRQLSTIVINDG
                     SKDKTEQILTENKIPHIQLIHNLGIGGAVQTGYKYAYENGFDIAIQFDGDGQHDITFV
                     DALVAPIENGNANMTIGSRFVEGSTSEFKSTKARQMGIRMISGFIKFFTKVKLYDVTS
                     GFRAIDKSLIKEFSMNYPTEYPEPISTTRVLKHGMKVAEVPVKMNERQGGTSSIHSWK
                     NAYYMINVLMSIINIGIWRD"
     gene            2052..2402
                     /locus_tag="ACZPDN_09485"
     CDS             2052..2402
                     /locus_tag="ACZPDN_09485"
                     /inference="COORDINATES: protein motif:HMM:NF021575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR1298890.1"
                     /translation="MPFNLRFAIFIVACILAFTVMRILHKDMIPVKYSLLWWIAILIL
                     VVLALWPDFFLFFVNLLRFQTTSNMVIGVFIVILIFITMSLTVIVSSQKNKINLLIQE
                     VSMLKQEIKDKANE"
     gene            2395..3642
                     /locus_tag="ACZPDN_09490"
     CDS             2395..3642
                     /locus_tag="ACZPDN_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR1298891.1"
                     /translation="MNNVYAYLLNKKVSLKENTLWYTMGTMCSSATSFLLMIYVTRIL
                     GVDEAGVFSISYSVGQLMLSIGWFGTRQFQVSDINEEFKFSDYLSLKLFMTIIMMVGC
                     LIYSVFLHFNTYKMLVTFLYCLFLICDVFADLFSARFQQVDKLFLSGMSYIIRILGYN
                     LVILFSLLCFKNLIVAIVLAMIYSALELTFFDLQLIKRISQIKIEFHMDKIIQLIKNC
                     FPLFISSFLTTFIVNVPKNAIELNFDSSVQTYYNIIFMPSYIINLFCMFIFVPLYTSI
                     ANTWLNSTKDKFINTVVKLMIFDVLLSLVVFAGCYFLGIPLLELVYGVDLHSVKSSFL
                     VLIVAGCFTSMNSILSYIFTVIRRQKFMIYIYVVAMVLAQVMVKTLTLNYGIFGASLD
                     YLIGIASITVMFMLGLVLVLRKD"
     gene            3653..4531
                     /locus_tag="ACZPDN_09495"
     CDS             3653..4531
                     /locus_tag="ACZPDN_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1298892.1"
                     /translation="MSNVLFKQYDPIVLKKLQGVQTQMLKDVLDVCEKQDLPCFLIYG
                     TAIGAVRHQGFIPWDDDIDVGMLRKDYDRLMEIFDKELGEHYELLTPEIDSRYACTVT
                     HVQRKNTVFISEMTKDLKCNCRIFMDIFPFDNVPESVEDQKRLLRKSTLYGKLLFLSG
                     SGSPHIPYTGLKYHILHFGCMVAHYGLKLFHITPKFLYKKFKKVSTSFNDVDCEYVTS
                     YEYTGCLKDKIKKADLLPLEKVPFESIEAYIPANNDEFLKKVYGDYMQIPPVEKRVNH
                     APLVIQFEGEEPIYGE"
     gene            4521..>4771
                     /locus_tag="ACZPDN_09500"
     CDS             4521..>4771
                     /locus_tag="ACZPDN_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1298893.1"
                     /translation="MANKKVCIFSAQHLPHMGGVERYTLNLCKKLIEKGNEPVVVTSK
                     IGDMPNFEIMHDTRVYRFNCYPFLKGRYPFLKKDSEYKK"
BASE COUNT         1496 a          571 c          899 g         1805 t
ORIGIN      
        1 tggtcacatt atttaacggc gtattcccaa agtagtgttt tattgcataa aaaagcattt
       61 gatgaacatg gtaatgtaat tttgtatgaa gatggttcga tttactttgt aagagagcat
      121 tctccaacta gatctacata tgcgattgaa tatcaggaat taaaagggca acagttggta
      181 tcatataaag gagatacgat aacacgtggt ccattaaaat tagctacttt tgcgtatgta
      241 ccacaaacat ttgggatgac attggctcgc ttgatgcatt taaatagtac gcaagttgcc
      301 tttgtaggac gtatttttgc gttattattc tatgcatttt taatttcgca aggaattaaa
      361 ttggctccgg aattcgcaaa acgtatttta ttagttgttt cattattgcc aatggtaatg
      421 cagcaagtat gttcttataa ttatgattca attttgtttg gaatttgttt ctttagtatt
      481 ggatatttat tgaatttgat tcatcgcgag gaaaaaatca ataaaaaaga ttttattatt
      541 ttagctttat gcgcaactgt aattgcttca atcaaattta tttatttacc ggttttcggg
      601 cttggtttgt tgattccaaa agagcgattt agaattaaaa gaggcaaaga attaacgatt
      661 ttgggtattc tatgtatatg tgcttttgct tttataggtg taacattatt taataaacga
      721 ttctgggatt tacatccaac aaatccaggt ttaccaaatg aatatatgac gtatagtatt
      781 tcatatctat tcacgcatgt taaggatgaa ttagttctat tgtcgcgtac agtgcaaaac
      841 tatacagctg attatatttc gcaaatgatt tcatctcctt taggttgggt ggaaatctca
      901 atgcctgcaa ctcaattgat gggctttgga tgtatgcttg ctttgtcttg tatgggtaca
      961 gaaaaagata agaagtatag tttgattgaa aatttatggt tagttgtttt atttacagga
     1021 gtagccttta cggtattgat tgctttacaa actagctata caccgacttt tttggatatg
     1081 gtatatggtg tgcagggccg ctatttctta ccaatacttc cattgttgat gattgccgta
     1141 agggggttgt ttgtattaaa agaggattcc actaaaattg agaatagttt gatagttgga
     1201 aacattttct ttcatattac agaggttagt acaatattat ttattgtcat aggacgatag
     1261 aataatttaa tagaatgtga taacatatag agcggttatt acattctttt ttaatggatg
     1321 ggggttacac aatggaggaa aaagtattaa tcattattcc tgcatataat gaagaggaaa
     1381 gtattttaaa tacatataaa acaattttag aatacaatca aactcatact cgtcaattga
     1441 gtacgattgt gatcaatgat ggatctaaag ataaaacaga acaaatttta actgaaaata
     1501 aaattccaca tattcaattg attcataatt taggtattgg tggagccgta cagacaggtt
     1561 ataaatatgc ctatgagaat ggttttgata ttgcgattca atttgatgga gatggtcaac
     1621 atgatattac atttgtagat gcgctagttg caccgattga aaatggcaat gcgaatatga
     1681 caattggttc acgttttgtc gagggttcta caagtgaatt taaatcgaca aaagcacgtc
     1741 aaatgggaat tcgtatgatt tctggattta tcaaattttt tacgaaagtt aaattgtatg
     1801 atgtaacatc tggttttcgt gccattgata aatctttaat taaagaattt tctatgaatt
     1861 atccaacaga atatccagag ccaatctcaa ccacaagagt attgaaacat ggaatgaaag
     1921 tagcagaagt acctgttaag atgaatgaaa gacagggtgg tacttcaagt atacatagtt
     1981 ggaaaaatgc atattatatg atcaatgttt taatgtcgat tattaatatt ggaatttgga
     2041 gggattagta tatgccattc aatttgagat ttgcgatttt tattgttgcg tgtattttag
     2101 cttttaccgt tatgcgtatt ttgcataaag atatgattcc tgttaaatat tctttattat
     2161 ggtggattgc gatccttatt ttagttgtat tggcactttg gccggatttc ttcttatttt
     2221 ttgtgaattt attaagattt caaacaactt caaatatggt gattggcgtt tttatcgtca
     2281 ttttaatttt tatcacaatg tcattaactg tgatagtatc ttcgcaaaag aataaaatta
     2341 atttattgat acaagaagtt tcgatgttga aacaagagat aaaggataag gcgaatgaat
     2401 aatgtatatg cgtatttatt aaataaaaaa gtttcgttaa aggaaaacac attatggtac
     2461 acaatgggta caatgtgtag ttcggcaacg agctttttat tgatgattta tgtgacacgt
     2521 attttaggtg ttgatgaggc gggtgttttt tcaatttctt atagtgtagg tcagttgatg
     2581 ttaagtattg gatggtttgg tacaagacaa tttcaagtat cagatattaa tgaagaattt
     2641 aagttttcag attatctttc tttaaaatta tttatgacca ttattatgat ggttggatgt
     2701 ttgatttatt ctgttttctt acactttaat acatataaga tgttagttac ttttttatat
     2761 tgtctatttt tgatttgtga tgttttcgca gatctctttt ctgcacgttt tcaacaagtc
     2821 gataaactat tcttatccgg tatgtcttat atcattcgaa tcttaggata caatttggtt
     2881 atcttgtttt ctttattatg ttttaagaat ctaattgttg cgattgtttt ggctatgata
     2941 tattcggcat tggaacttac tttctttgat ttacaattaa tcaaaagaat cagtcaaatt
     3001 aaaattgagt tccatatgga taagattatt caattgatta agaattgttt tccattgttt
     3061 attagttctt ttttaacaac atttattgta aatgtcccta aaaatgcgat tgaattgaat
     3121 tttgattcaa gtgtacagac ttattataat attatattta tgccttcata tattattaat
     3181 ttattttgta tgtttatttt tgtgccacta tatacatcta tcgcgaacac atggctcaat
     3241 tcaacgaaag acaaatttat taatactgtt gtcaaattaa tgatatttga tgtcttattg
     3301 tcattggttg ttttcgcagg atgttatttc ttaggcattc cattgttaga acttgtatat
     3361 ggtgtagatt tacatagtgt taaatcatct ttcttggttt tgattgttgc aggatgtttt
     3421 acaagtatga attcgattct atcttatatt tttacggtaa ttcgaagaca aaaatttatg
     3481 atctatatct atgtagtggc aatggtgctt gcgcaagtaa tggttaaaac tttaacgttg
     3541 aattatggta tttttggtgc gagtttggat tatttgatag gtattgcttc gataacagtg
     3601 atgtttatgc ttggtttggt attggtttta agaaaagatt agggtgagaa aagtgagtaa
     3661 tgtattattt aaacaatatg atcctatagt attaaagaaa ttacagggtg tacaaacaca
     3721 gatgttgaaa gatgtacttg atgtttgtga aaaacaggat cttccttgtt ttttgatata
     3781 tggtacagcc ataggtgcag ttcgtcacca aggttttatt ccttgggatg atgatattga
     3841 tgttggaatg cttcgtaaag attatgatag actaatggaa attttcgata aagaattagg
     3901 ggagcattat gaattattaa cgccagagat tgattctcgt tatgcttgta cagttacgca
     3961 tgtgcaacgt aaaaatacag tttttattag cgagatgaca aaagatttaa aatgcaattg
     4021 tcgtattttt atggatattt ttccttttga taatgtacct gaatctgttg aggatcaaaa
     4081 gcgtttatta agaaaatcaa cattatatgg aaagctatta tttctttcag gttcaggctc
     4141 acctcatatt ccttatacag gtttgaaata tcatatttta cactttggat gtatggttgc
     4201 tcattatggt ttgaagttat ttcatattac tccaaaattt ttgtacaaga aatttaaaaa
     4261 ggtgagtact tcatttaatg atgtggattg tgaatatgta acgtcttatg agtatacggg
     4321 ttgtttaaag gataaaatca aaaaggcgga tttattgccc cttgaaaagg ttccatttga
     4381 atctattgag gcgtatattc ctgcaaataa tgatgaattt ttaaagaagg tatatggtga
     4441 ctatatgcag attcctccag tggaaaaaag ggtcaaccat gcaccgttag tcattcagtt
     4501 tgagggggag gaaccgattt atggcgaata aaaaagtttg tattttttct gcacagcatt
     4561 taccacatat gggtggtgtc gaaagatata ctttaaattt atgtaaaaaa ctcattgaaa
     4621 aaggtaatga gccggttgtc gttacttcta aaattgggga tatgcctaat ttcgaaatca
     4681 tgcatgatac tcgtgtatat cgatttaatt gttatccttt tctaaaaggg cgttatccat
     4741 ttttaaaaaa ggatagtgaa tataaaaaga t
//
LOCUS       DBMDWL010000155         4754 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7506_length_4754_cov_4.483989, whole genome shotgun sequence.
ACCESSION   DBMDWL010000155 DBMDWL010000000
VERSION     DBMDWL010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7506_length_4754_cov_4.483989"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            166..1854
                     /locus_tag="ACZPDN_09505"
     CDS             166..1854
                     /locus_tag="ACZPDN_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR1298894.1"
                     /translation="MAYFLKKTKRNDRLYLSIYESFYSPETKNTRHKSFRKIGFVDAL
                     IEQGIDDPISHFQKEVNELNSKRETEKCRINFQQISDISPELCLGYVPIAKILNMLDV
                     KEHFGYLSSSRKFEFDVYDVFSALVYARVVAPLSKHQTFHDILPKLYVKKNFSYDQLL
                     EGLEFMGEEYEKLVEILTVATDDNFILDSSRSYFDCTNYYFEIDKESTLQKRGPSKEN
                     RRDPIVGMGLLLDAQMLPVGMKIFPGNESEKPVMRDLIHDLKSRNNIKGRTIQIADKG
                     LNCAKNIIEAIDNGDGYLFSKSVKTLPEKERQWVLLDDGFETVFDQNGEPVYKIKECI
                     DTFTYSYKDDYGNVITRNIKEKRTVTYNFSLAKKKLMEINRMIEKAKAHRACQAKKEE
                     YGESSKYMQFLDDQGKSIKPQLNQKAIDKDKELAGYNMLVTSEINMSSKDIYNAYHQL
                     WQIEESFRIMKSELDTRPVYLQKENRIKGHFFICYTAVLLSRILQFKILENKYSASTI
                     YDFMRKFRVVRINSTRFINLLTSKEFVTDLSKKLNQPITNYYLTDKQIKMMHTR"
     gene            complement(1908..2456)
                     /locus_tag="ACZPDN_09510"
     CDS             complement(1908..2456)
                     /locus_tag="ACZPDN_09510"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1298895.1"
                     /translation="MIVLASKSPRRKEILKALGYDFIVCPALKDEVFDLNLGLDEALK
                     KVAESKAKEVKESYLDSIIISADTIVCLDDKILGKPKSKKDAIHTLKSLSNRRHQVKT
                     GVCIVYKNQTFLHVETTDVYFKKLADEDILSYVNSGKCMDKAGSYGIQECDFVDHIEG
                     DYTNVVGLPKYVVETMMKDVAL"
     gene            complement(2453..3142)
                     /locus_tag="ACZPDN_09515"
     CDS             complement(2453..3142)
                     /locus_tag="ACZPDN_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIR1298896.1"
                     /translation="MRRVLSFLFGWCFIIVCVVFSIKSTALNPNFYIPKYEEMELASD
                     IGVSKKDLNQSIRLLLDYLDDKRDDIKGHITWYGVSQETFNEKETSHMVDVKVLYQNA
                     LKVAKMAFIILGLVVLYFYLNDKTLMFAYLSKGFLMAMFTFILMLVFFGFWILTDFTS
                     FWTWFHTIFFSNQLWLLDPNTDFMICMLPETIFYKLVLACVVKVICFMALASVLAIYY
                     MVKKAPIGFER"
     gene            complement(3142..3576)
                     /locus_tag="ACZPDN_09520"
     CDS             complement(3142..3576)
                     /locus_tag="ACZPDN_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1298897.1"
                     /translation="MSKYYVVSGDILPDVLEQVMQARILLQSGKAKRISEAVKMVGIS
                     RGTYYKYKDAVFSFNAEQSNRKAIISMILRNEKGTLSKVLSLVSVKQVNVLAINQTIP
                     INGIANVALTLDISDLEISIQSLVSLIEAMPMVEKADLVAVE"
     gene            complement(3569..4441)
                     /gene="thrB"
                     /locus_tag="ACZPDN_09525"
     CDS             complement(3569..4441)
                     /gene="thrB"
                     /locus_tag="ACZPDN_09525"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR1298898.1"
                     /translation="MISVHVPATSANCCVGFDCLGMAVDWWAHFTFEESDELIVEGCP
                     EEFKGSKNLVVQAFYTACDYLRLDYPTFKLTIDTDIPFARGLGSSSTCVVAGILACDA
                     WFKKNMDQLEILKIATSIEGHPDNVAPAIFGQATACFMEKEDVKMSLVPCADYHCLAI
                     VPCYEVKTSQARKVLPTKMAFKDCVSQVSHALVFIQALQQGDESLLASSCKDLLHEPY
                     RRQFIKEYDRIKTYCEQKQLPMWISGSGSTMMVVSLEKKRLIELSKFIDENLNGLDHR
                     CLTIAKKGAFIKYE"
     gene            complement(4434..>4754)
                     /locus_tag="ACZPDN_09530"
     CDS             complement(4434..>4754)
                     /locus_tag="ACZPDN_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1298899.1"
                     /translation="VYDKTCYVLDPHSAIAYKTAKECKDRPIVSLATASPYKFSQDVL
                     KALGYDEENEWKAMETLSKYCQDAIPSQLKELQDLDILHNKVIDVASMKDVVCQSTKE
                     VFHD"
BASE COUNT         1653 a          953 c          677 g         1471 t
ORIGIN      
        1 cccgtcttta gaggtttgca ccatgaatgt ttagaggcta ttctgagaaa aagttgagat
       61 tctccagtat ttatcgttgt tttttgtgct ttaaatttat tttatttttt ttatttattt
      121 ggtataatta tggtgtaaat gtgtaatgct tggagggcac tttctatggc ctattttctt
      181 aaaaaaacaa aacgaaatga cagactttat ctttccatct atgagagttt ttattctcca
      241 gaaaccaaga acacccgaca taaaagtttt cgtaaaatcg ggttcgttga tgctcttatc
      301 gaacagggta tagatgatcc aatctctcat tttcagaaag aagtaaatga actgaattct
      361 aaacgggaga cagaaaagtg tcgcatcaac tttcaacaga tttccgacat ttcaccagaa
      421 ctatgtctgg gttatgtacc aattgcgaaa atcttgaaca tgctggatgt aaaagaacat
      481 tttgggtatt taagctcttc aaggaaattc gagttcgatg tatatgatgt cttttccgct
      541 cttgtatatg ctcgtgttgt tgcaccttta tctaaacatc agacttttca tgatattctt
      601 ccaaagctat atgttaaaaa aaacttcagt tatgatcaac tcctggaggg cttggaattc
      661 atgggcgaag aatatgagaa actggttgaa atactgaccg ttgcgactga tgacaatttt
      721 attctggatt ccagccgttc ttattttgat tgtacaaact attactttga aatcgataag
      781 gaatctactc ttcaaaaaag aggtccttcc aaagaaaatc gaagggaccc tattgtggga
      841 atgggccttc ttttagacgc acagatgtta ccagtcggca tgaagatatt cccaggcaat
      901 gaatctgaaa agccggtcat gcgtgatcta atccacgatt tgaaatcaag aaacaatatc
      961 aagggccgta ccatccaaat tgctgataaa ggccttaact gtgctaaaaa tattattgaa
     1021 gctatcgata atggagatgg ctatctgttt tcgaaatctg taaaaaccct tcctgaaaaa
     1081 gaaagacaat gggttcttct tgatgacgga ttcgaaacag tgtttgatca gaacggagaa
     1141 cccgtttata aaatcaagga atgcatcgat acgtttacct attcatacaa ggatgattac
     1201 gggaatgtca taactcgtaa tataaaagaa aaaagaacag ttacctataa cttcagtctc
     1261 gcaaagaaga aactgatgga aatcaataga atgattgaaa aagccaaggc acaccgtgca
     1321 tgccaggcca aaaaagaaga atatggtgaa tcatcaaaat atatgcagtt tctagatgat
     1381 caggggaaaa gtataaaacc ccagctaaat caaaaagcta tagacaaaga caaagaacta
     1441 gccggatata atatgctcgt aacatctgaa atcaatatgt catcaaaaga catatacaat
     1501 gcttatcatc agctttggca aatcgaagaa tccttccgca tcatgaagtc cgaacttgat
     1561 acacgtccag tctatctaca aaaagaaaat cggatcaaag ggcatttctt catctgttac
     1621 acagccgtgt tattatcacg aatccttcaa ttcaagattc tggaaaataa atactcagca
     1681 agtacaatat acgatttcat gagaaaattc cgagttgtaa gaatcaactc cacaagattc
     1741 ataaatctat taaccagcaa agaattcgta acagatctat ccaaaaaatt aaatcaacca
     1801 ataacaaatt attatctaac agataaacaa ataaaaatga tgcatactcg ataatcaaat
     1861 atcgagtgtt gcaccatttt ttactattca acctctaaag tcaggtacta gagtgcaacg
     1921 tctttcatca tcgtttcaac gacatattta ggtaaaccta ccacattggt ataatccccc
     1981 tcaatatgat ctacaaaatc acattcctga atcccataag agcctgcttt atccatacat
     2041 tttccactat ttacatagga taaaatgtct tcatctgcca acttcttaaa ataaacatct
     2101 gttgtttcga catgtaaaaa tgtctgattt ttataaacta tacaaacccc tgttttcact
     2161 tgatgtctac gatttgataa agatttcaac gtatgtatcg catccttctt tgatttaggt
     2221 tttcccaata tcttatcatc caaacataca attgtatctg ccgatataat aattgaatct
     2281 aaataagatt cttttacttc tttcgccttt gattcagcca ctttttttaa tgcctcatct
     2341 aaacctaagt ttaaatcaaa cacttcatcc tttaaagcag gacaaactat aaaatcatag
     2401 cctaatgctt ttaatatctc ttttcttcga ggagactttg atgcaagtac aatcatcttt
     2461 caaaacctat tggtgccttt ttcaccatat aatatatcgc caaaacacta gctaaagcca
     2521 tgaaacaaat cactttaact acacaagcaa gtactagctt ataaaaaatt gtttctggaa
     2581 gcatacaaat cataaaatcc gtattgggat ctaataacca caattgattc gaaaaaaaga
     2641 ttgtatgaaa ccaagtccaa aagcttgtaa aatctgtcaa gatccagaat ccaaaaaaca
     2701 caagcatcaa tatgaatgta aacatggcca tcaaaaagcc cttacttaaa tacgcaaaca
     2761 tcaatgtttt atcatttaag taaaaatata aaactaccaa tcctaaaata ataaatgcca
     2821 ttttagctac ctttagagca ttttgataca aaactttcac atccaccata tgactcgttt
     2881 ctttttcatt aaacgtttct tgagatacac cataccacgt aatgtgtccc tttatatcat
     2941 cacgcttatc atccaaataa tccagtaaca aacgtatact ttgatttaaa tctttcttag
     3001 aaacaccaat atcactagcc agttccatct cttcatactt aggaatataa aaatttgggt
     3061 ttaatgctgt actcttgata ctaaaaacaa cacatacaat aataaagcac cagccaaaca
     3121 gaaaacttaa aacacgtctc attattctac agctaccaaa tcagcctttt ctaccatagg
     3181 catagcttca atcaaactca ccaaagattg tatactaatt tctaagtcgc taatatctag
     3241 agttaatgcc acattggcaa ttccattgat tggaattgtt tggttaatcg ccaacacatt
     3301 aacttgtttt accgaaacta aagataagac tttagaaagt gtaccctttt catttcgtaa
     3361 aatcatagaa atgattgcct tacgattgga ttgttcggca ttaaatgaaa aaactgcatc
     3421 tttatattta taataagtac cgcgagaaat acctaccatt ttaacagctt cactaattct
     3481 ttttgccttg cccgactgca ataaaatacg cgcctgcatg acttgttcta atacatctgg
     3541 taaaatatca ccactcacga cataatattt actcatactt tataaacgcc ccctttttgg
     3601 ctattgttaa acagcgatga tccaatccat tcaaattttc atctataaac ttagataatt
     3661 caatcaaacg ttttttttct aaagaaacaa ccatcattgt tgaaccacta cctgaaatcc
     3721 acattggaag ttgtttttgt tcacaatacg tctttatcct atcatattct ttaataaatt
     3781 gcctacgata tggttcatgt aatagatcct tacaagaact tgcaagtaaa gactcatccc
     3841 cctgttgcaa agcctgaata aagactagtg catgcgatac ttgtgaaaca caatctttaa
     3901 atgccatttt agtcggcaaa acttttcttg cctgagaagt tttaacttca taacaaggta
     3961 caatagctag acaatggtaa tctgcacacg gaacaagact cattttcaca tcttcttttt
     4021 ccataaaaca agctgttgcc tggccaaaaa tagcaggtgc cacattatca ggatgtccct
     4081 caatacttgt ggcaatcttt aaaatttcca actgatccat attcttttta aaccaagcat
     4141 cacaagctaa gataccagca acaacacaag ttgaacttga acccaacccc ctcgcaaatg
     4201 gaatatctgt atcaattgtg agtttaaaag taggataatc caaacgcaaa taatcacatg
     4261 ccgtataaaa agcttgtaca actaaatttt tactaccctt aaattcttct ggacatcctt
     4321 ccacaatcaa ttcatcactt tcctcaaaag taaaatgtgc ccaccaatcc acagccattc
     4381 ctaaacagtc aaatcccacg caacaatttg cacttgttgc cgggacatgt acactaatca
     4441 tggaatacct ctttagtact ttggcatacg acatctttca ttgaagctac atcaatcact
     4501 ttattatgta aaatatctaa atcttgaagt tcttttaact gagaaggaat tgcatcttga
     4561 caatactttg ataatgtttc catagctttc cattcatttt cttcatcata tcctaatgcc
     4621 tttaaaacat cttgactaaa tttatatgga gaagccgttg ccaaagaaac aataggacga
     4681 tctttacatt cttttgcggt tttatatgcg attgcagaat gaggatctaa gacatagcaa
     4741 gtcttatcat atac
//
LOCUS       DBMDWL010000156         4743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7524_length_4743_cov_3.364283, whole genome shotgun sequence.
ACCESSION   DBMDWL010000156 DBMDWL010000000
VERSION     DBMDWL010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7524_length_4743_cov_3.364283"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="ACZPDN_09535"
     CDS             complement(<1..367)
                     /locus_tag="ACZPDN_09535"
                     /inference="COORDINATES: protein motif:HMM:NF018682.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HIR1298900.1"
                     /translation="MKLAVIADDFTGGADAASFLKRQNAKVVLVTKIPHEQVDCDCLV
                     FALKIRSIPKAEAIESVKLVCEYLKSHPVEHLYYKYCSTFDSTPKGNIGPILDFLLEY
                     YHLNRCIICPSLPENGRTVE"
     gene            complement(364..831)
                     /locus_tag="ACZPDN_09540"
     CDS             complement(364..831)
                     /locus_tag="ACZPDN_09540"
                     /inference="COORDINATES: protein motif:HMM:NF015444.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIR1298901.1"
                     /translation="MIRFNEACGNAGKVVTARLLPDTDLIEGIEEICKSNQIKYAYVS
                     CFGSFKKCGYMYLVPKSTAKVKAGYGDIITKEGPIEFLNGTGVVCQNNNKYDTHFHAT
                     MCDQNGTVFGGHIVKGHTPTLTTVDLIIMEIKDVEMLRVYDEETDLTQFLPQK"
     gene            complement(845..1684)
                     /locus_tag="ACZPDN_09545"
     CDS             complement(845..1684)
                     /locus_tag="ACZPDN_09545"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR1298902.1"
                     /translation="MLVTMKEILLHAKENNYGVTAPNIQNEDMARAVVRVAEKCNAPM
                     IIDINSWVHPDIPWLVNFCRDLAIRSTVPIAINLDHGKNYHDMILGIRSGFTSIMVDR
                     SSLPYEENVTQTKEVVKMCHELNISVEAELGHVGQGEQYDTDGISNLTVPEEAAQFIK
                     ETGVDCLAVAIGTAHGRYSGVPHIDFDRLAKIVELCPDTPLVLHGGSGTGDANLKKAV
                     HSGIQKVNLATELVVAGRDTLAEFIQNPKFDKWELMPSFIKGYSDKLEHYVRLFDQVD
                     KAW"
     gene            complement(1804..2658)
                     /locus_tag="ACZPDN_09550"
     CDS             complement(1804..2658)
                     /locus_tag="ACZPDN_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1298903.1"
                     /translation="MKIIQSSVIDNIKVNLESLFPAEKKVAQYILEHLNEVIFLNISE
                     LATNSNSSESSVVRMAKHLGYNGYFQMRLLLSNDISKKDKDILSNQPLLTSEKIFSSC
                     ANRIQSLSNMISTEQLVKAAKLIQNARICHITSIGNTIPVAIDLGFRLERNGQACTYS
                     NQPEQYYNHIALGHKDDLLIAITRSGASKQVLKSISLAEKKGMKILIITASLNEEFTK
                     KADCILQINDTNEIINQKVRADSHLLEIALNDALIYVVKNYLLFDNSFEQSQNLNEVE
                     LLLSETKL"
     gene            2937..3833
                     /gene="gnd"
                     /locus_tag="ACZPDN_09555"
     CDS             2937..3833
                     /gene="gnd"
                     /locus_tag="ACZPDN_09555"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR1298904.1"
                     /translation="MKLKMVGLGKMGLNLALNMKEHGVDVEGFDVNEEARKKAESENI
                     KTYASLEDVASKDEKNVIWVMLPAGKITNSVLLQLVELCKANDIVIDGGNSDHRDSLK
                     TAHLMEEKGIYFFDIGTSGGVYGARHGASFMCGGDPDVFKNELQEMLESIATTNGCLY
                     TGKTGSGHYLKMIHNAMLYGYMQTLGEGFELLEKSEFDYDLQNVADSLSKSSVIRGWL
                     LELAADAFKKDPDLSKIKGVVGASKTTGWTIESACELGVPIPVISTSLMMRLRSKQDD
                     SFSAKVIASLRDEVGGHKAQSK"
     gene            3844..4149
                     /locus_tag="ACZPDN_09560"
     CDS             3844..4149
                     /locus_tag="ACZPDN_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298905.1"
                     /translation="MWFYKIEKEGKDIHVTWNTTMTYLKNTFGFVGIVACLFNVVWLA
                     TICLGTLAIALAYFVYRYGDLVKILHGLERKDILKYTGSQYSFKDPLIVTIPRVNSL"
     gene            4146..4679
                     /locus_tag="ACZPDN_09565"
     CDS             4146..4679
                     /locus_tag="ACZPDN_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016901.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIR1298906.1"
                     /translation="MNRQEELIRILLKSKKPLTTTELAEMLDVSSRTIRSDLVKIETE
                     LLVHHLCLEKKPHVGVWIQGAKEDKVALFLNVDQQNSTVNTYSKDYRIGCILVQILLG
                     NSKIYPDKFADELYVSRSTIEKDLSAVSKWLEKHQLELSRNANNGLYVKGNEENIRNA
                     VGSLANDLNTRNLYYIR"
BASE COUNT         1592 a          729 c          821 g         1601 t
ORIGIN      
        1 tttcaacggt tcttccattt tccggtaatg aaggacaaat gatacaccta tttaaatgat
       61 agtactcaag taaaaaatct aatataggcc ctatatttcc tttaggcgta gaatcaaatg
      121 tggaacaata tttatagtat agatgttcaa cgggatgcga ttttaaatat tcacaaacca
      181 gtttcacaga ttctattgct tcggctttag ggatactacg tatttttaat gcaaatacca
      241 aacaatcaca atctacctgt tcatgtggta tttttgttac tagaacaact ttcgcatttt
      301 gtctttttaa aaaactagcc gcatcagctc caccggtaaa atcatccgct ataacagcta
      361 acttcatttt tgtggaagaa actgcgttaa atctgtttct tcatcatata ctcgtaacat
      421 ttctacatct ttgatctcca taattattaa atcgacagta gttaatgtgg gagtatgccc
      481 cttgacaata tggccaccaa atactgttcc attttgatcg cacatagttg catgaaagtg
      541 ggtgtcgtat ttattgttat tttgacaaac aacgcctgtt ccatttagaa attcaatagg
      601 gccttcttta gtgataatat ctccataacc tgcttttact ttagccgttg atttaggaac
      661 caaatacata tatccacatt ttttaaaact accaaagcaa gaaacatatg cgtattttat
      721 ttgattgctt ttacaaatct cttcgattcc ttcaattaaa tcagtatcag gcaataatct
      781 agcagtaact actttcccag catttccaca agcttcatta aaacgtatca tatttaaatc
      841 ctccttacca agccttatct acttgatcaa ataaacgtac ataatgttct aacttatctg
      901 aatatccttt aataaatgaa ggcattaatt cccatttatc aaatttagga ttttgaatga
      961 attctgcaag tgtatctctt ccggcaacaa caagttcagt tgctaaattc actttttgga
     1021 taccactatg aacagctttt tttaaattgg catcgcccgt acctgatcct ccatgcaata
     1081 ctaatggagt gtctgggcaa agctccacaa tctttgctaa acgatcgaag tcaatatgtg
     1141 gaacaccact gtatcgacca tgagcagttc cgattgcaac agccaaacag tctactcccg
     1201 tttctttaat aaattgtgcg gcctcttcag gtacagtaag attagaaatt ccatctgtgt
     1261 catattgttc gccttgacca acatgtccta attcagcttc aacactgata tttaattcat
     1321 gacacatttt taccacttct ttagtttgag taacattttc ttcgtaaggt aaacttgaac
     1381 gatcaaccat aatagatgta aatcctgagc gaattcctaa aatcatatca tgatagtttt
     1441 ttccatgatc taaattaatg gcgattggta cagtagagcg aatggctaaa tctctacaaa
     1501 agtttactag ccatggaata tctggatgta cccatgaatt aatgtcaatg atcatagggg
     1561 cattacattt ttctgcaacg cgaacgactg ctctagccat atcttcattt tgaatatttg
     1621 gtgcagtaac tccataattg ttctctttag catggagtaa aatttctttc attgttacaa
     1681 gcatattatt taatctcctc gtgaaatata attttatgga ttagctaatt ccaaattcag
     1741 tatagaaata aattttatat atgtcaatga aaaaagagat gaacgaaata atatttcatc
     1801 tctttatagc tttgtttcgc ttaataaaag ctctacttca tttagatttt gagattgctc
     1861 aaaactatta tcaaaaagaa gataattctt aacaacataa attaaagcat cgttcagagc
     1921 aatttctagc aggtgagaat cagcacgaac tttttgattt attatctcat ttgtatcgtt
     1981 aatttgaaga atacagtctg cttttttagt gaactcttca ttcaaggagg ctgtaatgat
     2041 aagaatcttc atgccttttt tctcggctaa agatattgat tttaaaactt gttttgatgc
     2101 accagagcga gtaatagcga ttagtaaatc atctttatgg cctaatgcaa tgtgattata
     2161 gtattgttct ggctgatttg aatatgtaca agcttgtcca tttctttcta aacgaaatcc
     2221 tagatcaatt gcaactggaa tggtgtttcc aatactagtg atatggcaaa tgcgagcatt
     2281 ttgaatcaat tttgcagctt tgactaattg ttctgtactt atcatatttg ataaagattg
     2341 aatacgattt gcacaggaac taaatatttt ttcactcgtt aataaaggct gattactcaa
     2401 aatatcttta tcttttttgc taatgtcatt tgaaagaagt aatctcattt gaaagtatcc
     2461 attgtatcct aaatgtttag ccattcttac aacggaagat tcactcgaat ttgaattagt
     2521 agccaattct gagatattta aaaaaattac ctcatttaga tgttcaagaa tatattgagc
     2581 tacttttttt tcagctggga ataaggattc taagtttact tttatattgt ctataactga
     2641 agattgtata attttcataa aattcctctt ttcatgtaat ataatcagca atataaaaac
     2701 gattacatta gtaaacgtta atatttgcta atttttttat atagtatatc atactatata
     2761 tgattgattt tgacttaatg tggaaatcac tttgcggaaa ccatcatgaa tgtggaaaaa
     2821 caaatgtgga aacacgaatc ggatgaataa aagcttgatt tatgtacagc gcttacattt
     2881 tttattatga tgtacctgtc aaaagacata gggatcaaaa gaaaggaggg tacgtcatga
     2941 aattaaaaat ggtaggtctt ggtaaaatgg gcttaaatct tgcattgaat atgaaagaac
     3001 acggtgtaga tgttgaagga tttgatgtta atgaagaagc tcgtaaaaag gctgaatcag
     3061 aaaacattaa gacgtacgca tctttagagg acgttgcttc taaagatgaa aaaaatgtga
     3121 tttgggtaat gttacctgct ggaaaaatta caaattctgt attgcttcaa ttggtagagt
     3181 tgtgtaaagc aaatgatatt gtaattgatg gaggaaactc agatcataga gatagcttga
     3241 aaacagcgca tttgatggaa gagaaaggta tttacttctt tgacattgga acaagcggtg
     3301 gcgtttatgg agcaagacat ggagcaagct ttatgtgtgg aggagatcca gacgtattta
     3361 aaaatgagct tcaagaaatg ttggaaagta ttgctactac aaacggatgc ttgtacacag
     3421 gaaaaactgg tagtggtcac tatttgaaaa tgattcacaa tgcaatgcta tatggctata
     3481 tgcaaacatt aggtgaagga tttgaattgt tggaaaagag tgaatttgat tatgacttac
     3541 aaaatgtcgc agattctttg agtaaatcaa gtgtaattcg tggatggtta cttgaacttg
     3601 cagcggacgc attcaagaaa gatccagatt tatcaaaaat caaaggtgtt gttggagcaa
     3661 gtaaaacaac aggttggaca attgaatccg cttgtgaatt aggtgttcca attccagtaa
     3721 tctcaacatc attaatgatg cgtttacgtt caaaacaaga tgattcattc tcggctaaag
     3781 tgattgcctc tttacgtgat gaagtaggag gacacaaagc tcagtcaaag taaaggagtg
     3841 cctatgtggt tttataagat tgaaaaagaa ggaaaagata tacatgttac atggaataca
     3901 acaatgacat atttaaaaaa tacttttgga tttgttggta tcgtagcttg tctgtttaat
     3961 gtagtatggt tagcaaccat ttgtttagga accttagcta tagctttagc gtattttgta
     4021 tatcgctatg gtgatctggt taaaatttta catggtttag aacgtaaaga tatattgaaa
     4081 tatactggta gtcagtattc gttcaaagat ccattaattg taactatccc tcgggtgaat
     4141 agtctatgaa tagacaagag gaattaatac gaattctttt aaaatcaaaa aaaccactta
     4201 ctacgacgga attagctgaa atgttggatg taagtagtcg aacaattcgg tctgatttag
     4261 taaaaattga aacggaatta ctggttcatc atttgtgtct tgaaaaaaag cctcatgtag
     4321 gcgtttggat tcaaggagca aaagaggata aggttgcatt atttctaaat gtagatcaac
     4381 aaaatagtac agtaaataca tattcaaaag attatagaat aggctgtata ttagtacaaa
     4441 ttttactagg aaatagcaaa atttatcctg ataaatttgc agatgaatta tatgtaagtc
     4501 gttctactat tgaaaaagac ttgtctgcag tttctaaatg gttggaaaaa catcagttgg
     4561 agttatctag aaatgcaaat aatggattgt atgttaaagg caacgaagaa aatattcgta
     4621 atgcggttgg aagcttagct aatgatttaa atactcgaaa tttatattat atcagataaa
     4681 taaaaagcct gctgtgaaaa tccacaacag gctttattta gttttattat atttattttt
     4741 tat
//
LOCUS       DBMDWL010000157         4724 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7571_length_4724_cov_2.980965, whole genome shotgun sequence.
ACCESSION   DBMDWL010000157 DBMDWL010000000
VERSION     DBMDWL010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_4724_cov_2.980965"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            213..2765
                     /locus_tag="ACZPDN_09570"
     CDS             213..2765
                     /locus_tag="ACZPDN_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1298907.1"
                     /translation="MNIEQMSERLQKILMDAINLAKSNQHPSVDTVDMLETIFKDDVL
                     DGLFERIGLDKARALQMVQKESNRVAKSSTSNINLSNEVQKSFEDAQNWASQHEETYL
                     SVASVFLALLFNRSYISKELVRTFNLNKDACFKAELERRNGKKFDTPNSEENVEALKK
                     YGRDLVQDVRDGKIDPIIGRDDEIRRVMQILSRKTKNNPVLIGEPGVGKTAVVEGIAW
                     RIMKGDVPESLKEKRLIELDMGSLIAGAKYRGEFEERLKSILDEVKKSDGQIILFIDE
                     IHNLVGAGKTEGSMDAANLLKPMLARGELHCIGATTFNEYRKYIEKDAALERRFQKVQ
                     VAQPSVEDTISILRGLKDRFESYHGVRILDEALIAAATMSDRYITDRFLPDKAIDLVD
                     EACATLKVEMESMPQELDELQRKILQLQIEKTSLQKEEDKRAVERREEIEGELASLQS
                     KRDEMHSKWEDEKRGLANEKEDKQRLEKARLDLEQARNEARYEDAAKLQYGMIPELEA
                     RIQKEQASQKEDALIQETVNEELIAKIVSRWTGVEVTRLVASEREKLLNLKAELEKRV
                     VGQDEALELVTDAILRSKAQIQDENRPIGSFMFLGPTGVGKTEVAKALAEQLFDDERH
                     IVRIDMSEYMEKHSVARLIGAPPGYVGYDEGGQLTEAVRRNPYSIVLFDEIEKAHPDV
                     FNVLLQILDDGRITDSKGVTVDFKNTIIILTSNLGSQYAFESGNIHEKYMEEVKRHFK
                     PEFINRIDEIIVFNALNDSAFIKIAHKFVGLLQKRLADRDITLNVSDAVYNQIAKMGV
                     DPVYGARPMKRYIQRNLETQIAKEMIKTGAMQGDVIHVDCQDGEYTFDIQGR"
     gene            2824..3558
                     /locus_tag="ACZPDN_09575"
     CDS             2824..3558
                     /locus_tag="ACZPDN_09575"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1298908.1"
                     /translation="MSLKDWIQESNNIVFFGGAGVSTESNIPDFRSDNGLYKKKYPYP
                     AEIMLSHSFYLSHPKEFFDFYFNQMIYSDAKPNKAHLALSKLEQMGKLKAIVTQNIDG
                     LHQMAGSKHVYELHGSVLRNTCTHCQTKYSLEDMMKYRDSDGIPRCPKCGAIIKPDVV
                     LYEEGLDEYTLYSAVHAIEQADLLIVGGTSLVVYPAAGLINYFHGKHLVLINRDSTNM
                     DSKADLVIHDSIGKVLDDSLCEVYVK"
     gene            3603..4142
                     /gene="epsC"
                     /locus_tag="ACZPDN_09580"
     CDS             3603..4142
                     /gene="epsC"
                     /locus_tag="ACZPDN_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIR1298909.1"
                     /translation="MSEKSKRSFIRDGLYNINRTMQLDPAAHSKWEVFWLYPHIKAIG
                     LHRMAHWFWVHNHTFIARWVSNRARHWTGIEIHPGAVIGRGLMIDHGMGVVIGETAII
                     GDDCQLYHGVTLGGTGKQHVKRHPTLEDGVYIGCGAKCLGNITLKKGCRIGANAVLLK
                     DVPEGATAVGIPARIIEKK"
     gene            4151..>4724
                     /locus_tag="ACZPDN_09585"
     CDS             4151..>4724
                     /locus_tag="ACZPDN_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298910.1"
                     /translation="MWKYRCKSHLIAMSVAFVVGLGLSAVVFSSGVDLNSDMFSSALS
                     QAPGVNTEALNQVLTYLQNNMWILYLGDALLISGIINIIYIGQYVTARFNISPWIVMC
                     LIFFLPEYMIYIGTLLVIPAYIVCIYGMLSLRSSICKERREFNFSSDDELVRMYKIHH
                     ELDESYKDLAKTCRKNVRKLTGIYALGIVAL"
BASE COUNT         1523 a          619 c         1065 g         1517 t
ORIGIN      
        1 caaacatatt ataccaaaaa agaccgatat tcagattttt aaatcttgaa tattcggtcc
       61 ttttttatta agctgaaact tctttatccg ttacagctcc ttttcttttt caaaaaatat
      121 tagcacttaa gtgttgacag tgccaaaaag agtgttatac tatcattagc aatcataaag
      181 agtgagtgct aagtacaagg aggtgcttat ttatgaatat cgaacaaatg tcagagcgtt
      241 tacagaaaat tttaatggat gcgatcaatt tagcgaaatc taaccaacat cctagtgtgg
      301 atacagtgga tatgttggag acgattttta aggatgatgt attagatggt ttatttgagc
      361 gtattggctt agataaagcg cgtgctttac agatggttca aaaagaatca aatcgtgtcg
      421 caaagagctc gacaagtaat ataaatttat caaatgaagt ccaaaaatca tttgaagatg
      481 cacaaaactg ggcaagtcaa catgaagaga cttatttatc ggttgccagt gtattcttag
      541 ctttattatt taatcgttct tatatttcta aagaattggt aagaactttt aatttgaaca
      601 aggatgcttg ttttaaagct gaattagagc gtcgtaatgg aaagaaattt gatacgccaa
      661 attcagaaga gaatgtggaa gctttaaaga aatatggtcg tgatttagtc caggatgtgc
      721 gcgatggtaa gattgatcca atcattggtc gtgatgatga gattcgtcgt gttatgcaga
      781 ttttatcgcg taagacaaag aataatcccg ttttaattgg tgagccaggc gttggtaaaa
      841 cagccgttgt tgaaggtatt gcttggcgta ttatgaaggg ggacgtacct gagtctttaa
      901 aagagaaaag attgattgaa ttagatatgg gatctttgat tgcgggtgct aagtatcgtg
      961 gtgaatttga ggagcgttta aaatccattt tggatgaagt gaaaaaatcg gatggacaaa
     1021 tcatcttatt tattgatgag attcataact tagttggtgc tggtaaaaca gaaggtagca
     1081 tggatgcggc taacttatta aagccaatgt tagctcgtgg tgaacttcat tgtattggtg
     1141 caacaacatt taatgaatat cgtaagtata ttgaaaagga tgcagcttta gagcgtcgtt
     1201 tccaaaaagt gcaagttgca caacctagtg ttgaagatac aattagtatt ttgcgtggat
     1261 tgaaagatcg ttttgaaagc tatcatggtg tgcgtatttt ggatgaagct ttaattgcgg
     1321 ctgccacaat gtcggatcgt tatattacag atcgtttctt gccagataaa gcgattgatt
     1381 tggtagatga ggcttgtgct acattaaaag ttgaaatgga aagtatgcct caagaattgg
     1441 atgaattaca gcgtaagatc ttgcagcttc aaattgaaaa gacttcactt caaaaagaag
     1501 aagataaaag agcggttgag cgtcgagaag aaattgaagg tgaattggct tctttacaat
     1561 caaaacgtga tgagatgcat tctaagtggg aagatgaaaa gcgtggtttg gccaatgaaa
     1621 aagaagataa acaacgtttg gagaaggctc gtttagattt ggaacaagct cgtaatgaag
     1681 ctcgttatga agatgcagct aaacttcaat atggaatgat tccagaatta gaggctcgta
     1741 ttcaaaaaga acaggcaagt caaaaagagg atgcattaat tcaagaaacg gtaaatgagg
     1801 aattgattgc gaaaatcgtt tctagatgga caggtgttga agttacgcgt ttggttgcga
     1861 gtgaaagaga aaaattgttg aatttgaaag cagaacttga aaaacgtgta gttggtcaag
     1921 atgaagctct cgaacttgta acggatgcga ttttgcgttc aaaagcacag attcaagatg
     1981 aaaatcgtcc aattggatct ttcatgttct taggccctac aggtgttggt aaaacagaag
     2041 ttgccaaagc tttggcagaa cagttatttg atgatgaaag acacattgtg cgtattgata
     2101 tgagtgagta tatggaaaag cacagtgtgg cacgattaat tggtgcacct ccaggatatg
     2161 taggttatga tgaaggtggt caattgaccg aggcagttcg tcgtaatcca tatagtattg
     2221 tcttatttga tgaaattgaa aaggcacatc cagatgtatt taatgtgcta cttcaaatct
     2281 tggatgatgg tcgtattacc gattcgaaag gtgttactgt tgattttaag aatacaatta
     2341 ttatcttaac gtcaaattta ggtagtcaat atgcctttga atctggcaat attcatgaaa
     2401 aatatatgga agaagtaaaa cgtcacttta aaccggaatt tattaatcgt attgatgaaa
     2461 ttattgtgtt taatgcatta aatgattcag cctttattaa gattgcccat aaatttgttg
     2521 gtttactaca aaaacgtttg gcagatcgtg acattacatt aaatgtaagt gatgccgtat
     2581 acaatcagat tgccaagatg ggtgtagatc ctgtgtatgg ggctcgtcct atgaagcgtt
     2641 atattcaaag aaatcttgaa acacagattg ccaaggaaat gatcaaaact ggtgctatgc
     2701 aaggagatgt aatccatgta gattgtcaag atggtgagta tacatttgat attcaaggaa
     2761 gatagtgaat tatcttcctt tctttttgga tttcgaggta taatagggtc aggaagtgat
     2821 tttatgtctt taaaagattg gattcaagaa agtaataata tagtattttt tggaggagct
     2881 ggtgtgtcta cagaaagtaa cattccagat tttcgtagtg ataatggatt gtataaaaag
     2941 aagtatcctt atcctgctga aattatgttg tcacatagtt tttatttaag tcatccaaaa
     3001 gagttttttg atttttattt taatcagatg atttattcag acgcaaagcc gaataaggcg
     3061 catcttgctc tttcaaaact ggaacagatg ggtaaattaa aggctattgt tacgcaaaat
     3121 attgatggtt tacatcagat ggcgggatct aaacatgtat atgagcttca tggaagtgta
     3181 ttaagaaata catgtacaca ttgtcaaacg aagtattctt tagaagatat gatgaaatat
     3241 agggatagcg atggtattcc tagatgtcca aaatgtggtg ccattattaa gccagatgta
     3301 gtcttatatg aagagggctt agatgaatat actttgtatt cagccgttca tgcgattgaa
     3361 caggcagatc ttttaattgt aggtggaaca agcttggttg tatatccggc agctggtttg
     3421 attaattatt tccatggtaa acatttggtg ttaataaata gagacagtac gaacatggat
     3481 tcaaaggcag atttagttat tcatgattca attggaaaag ttttagatga ttcactatgt
     3541 gaagtttatg tgaaataaac actaaacttg aaatttaaac acaaatcaac taaaatttta
     3601 tcatgtcaga aaaaagcaag agatctttta tccgtgatgg attatataac ataaatcgaa
     3661 caatgcagct tgatcctgca gcccattcta aatgggaagt gttttggcta tatccgcaca
     3721 tcaaagcgat tggcttgcat cgaatggccc attggttttg ggtgcacaat catacattta
     3781 ttgcccgttg ggtaagtaat cgagcaaggc actggacggg tattgaaatt catcctggtg
     3841 ctgtgattgg tcgtggattg atgattgatc atggtatggg tgttgtgatt ggtgaaacgg
     3901 caattattgg tgatgattgt cagctttatc atggcgttac actaggtgga acaggtaagc
     3961 aacatgtaaa aagacatcct actttagagg atggtgtata tattggttgt ggggccaaat
     4021 gtttaggaaa tatcacttta aaaaaaggtt gtcgtatagg ggccaatgcg gtacttttaa
     4081 aagatgtacc tgaaggtgca acagccgtgg gtattcctgc aagaattata gagaagaaat
     4141 aggtggttat atgtggaaat atcgttgtaa atcccattta atagcgatga gcgtcgcttt
     4201 tgtggttggc ttaggcttga gtgcagttgt attttcaagt ggagtggatt taaattcaga
     4261 tatgttttca agtgctttaa gtcaagctcc aggtgtaaat acagaagcat tgaatcaagt
     4321 tttgacctat ctacaaaata atatgtggat tttatatctt ggggatgccc tattgatttc
     4381 ggggattata aatattattt atattggtca atatgtaacg gctcgtttca atataagtcc
     4441 atggatcgta atgtgtttaa tatttttctt accagaatat atgatttata ttggaactct
     4501 acttgtgatt ccagcctata ttgtttgtat atatggaatg ttgagtttac gctctagtat
     4561 ttgtaaagag cgtagagaat ttaatttttc aagtgatgat gaattggttc gtatgtataa
     4621 gattcatcat gaattggatg agtcttataa ggatctcgca aaaacttgcc gtaaaaatgt
     4681 gcgtaaatta acaggtattt atgcgttagg tattgttgcc ctat
//
LOCUS       DBMDWL010000158         4719 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7582_length_4719_cov_2.690992, whole genome shotgun sequence.
ACCESSION   DBMDWL010000158 DBMDWL010000000
VERSION     DBMDWL010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7582_length_4719_cov_2.690992"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(66..1049)
                     /locus_tag="ACZPDN_09590"
     CDS             complement(66..1049)
                     /locus_tag="ACZPDN_09590"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR1298911.1"
                     /translation="MTTIAIAGATGAVGQQMLECLKERNIQANLKLLASARSKGKEID
                     GHIVEELTQESFQGVDYVLGATGNDITKMWMPWAKEAGCIVVDNSSAFRLDQDVPLVI
                     PEVNKEDIKNHHGLIANPNCATIIALVALQALHEKYHIKRMIVTTFQAVSGAGVQGIE
                     DLKNQIEDENIPSKAFPYQIAFNLIPQIGDFDNLGISKEEWKMQNEARKMWHDDALKV
                     NCTCVRVPIWRSHSESITIECENEVDVNEARALLSKAEGVLLKDDPKNQIYPMPKDTT
                     NQDLVYVGRIRKDITDETNHTLSLFCCGDQIRKGAATNAVQILDELLKAND"
     gene            complement(1046..2365)
                     /locus_tag="ACZPDN_09595"
     CDS             complement(1046..2365)
                     /locus_tag="ACZPDN_09595"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1298912.1"
                     /translation="MLKITKFGGSSVANAEQFKKIKNIIQQDPTRRYIVVSAPGKRFS
                     ADNKITDLLYLLDAHRKYHVDASSVFKLIKNRFIEIKNELGLKQPIEKELDAFYTNLN
                     QMSQAVIVSRGEYFCAKLMAEYLGYAFVDAKDIIRFKLDKSIDWDATSAKLQAKYAQY
                     DHFVFPGFYGTSANGHIQVFPRGGGDITGAILAKCLHADVYENWTDVSGFLMADPTIV
                     QNPKGITHITYSELRELSYMGASVLHEEAIFPVKEANIPIHILNTNRPQDAGTIIQEH
                     SKIKSGYPITGIAGKKDFMSIYIYKKHMSNEVGFIRKALSILEKYHISIEHIPSGIDS
                     FNIVVEKKEIEESLYEILAHFKEELKPDKLTVQDDLALISTVGKNMSDKPGTSGKLFG
                     ALGKNNINIVMIAQGSDEINITVGVKKKDFTKTVQVIYDTFVGGNEE"
     gene            complement(2371..3483)
                     /locus_tag="ACZPDN_09600"
     CDS             complement(2371..3483)
                     /locus_tag="ACZPDN_09600"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1298913.1"
                     /translation="MNAVILGYGVVGKGIEILTNSLEDINIKYVYVRKEKENLPYFSN
                     NEDMVVQDDIDIVFECLNGLEPANTLIQKALKKGKHVISSNKAVVATYLDTYLELEKE
                     YGGSIQVEACVAGGIPFIDALLKLKRLEPLQGYEGIFNGTSNYILDSMQKNNLDFEDV
                     LKQAQEKGYAEKDPSNDIDGVDVYYKTKISNSLAFNTPIVDIPYYAGIRTIKMLDIKF
                     IKMNNKVLRHLSISRQIGDKIISIIAPCCMDENDFLANVPSNYNAQKILSHSFDTLGY
                     YGQGAGQLPTAQAMVQNALDTLEDKERKIVYAKPKEFDTSLLKETWLIRSDIALENYY
                     PIQKKEANYYWIENQDMSIIKKVKELDKNSFIALWR"
     gene            3574..>4719
                     /gene="thrC"
                     /locus_tag="ACZPDN_09605"
     CDS             3574..>4719
                     /gene="thrC"
                     /locus_tag="ACZPDN_09605"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1298914.1"
                     /translation="MNDYISTRDAHQSVSSKQAILNGMSPDGGLYVLPTLDQIHLDLS
                     LICTMSYKENAVYILSHLLTDYTKEELEACVENAYANSFSKQEITPIKKVDDVYVLEL
                     FHGPTCAFKDVALTLLPQLMSCALKSTSQKALILTATSGDTGKAALSGFCDVENIGIN
                     VFYPYKKVSAIQYRQMVTQKGKNVNVFGIQGNFDDAQSQVKRLFLDEELNEYCAKQNI
                     LLTSANSINVGRLVPQIVYYFDSYKQLVLQGDIQLGDKVSFSVPTGNFGDVLAGYYAY
                     LMGLPVEKFYVASNANRVLTDFLTTGIYDRNRDFIQTISPSMDILISSNLERLLYYAS
                     NKDTQKVSKWMQELNEKGRYQIDDETFKTIQNLFACNSVDDENTSLEI"
BASE COUNT         1417 a          789 c          810 g         1703 t
ORIGIN      
        1 atttttatac atcaacatat caccaatgca tctgttaagc caattaaaaa aataggcaag
       61 cgaaattaat cgtttgcctt taatagttca tctaggattt ggactgcatt tgtagcagca
      121 cctttacgaa tttggtcacc acaacagaat agagataatg tatgatttgt ttcatcggta
      181 atatctttac gaattctacc cacatagact aagtcttgat ttgtcgtatc ttttggcatt
      241 ggatagattt gattctttgg atcatccttt aataatacgc cttctgcctt gcttaatagt
      301 gctcttgctt cattgacatc aacttcattt tcacattcga tcgtgatgct ttcagagtga
      361 gaacgccaaa ttggaacacg gacacaagta cagttaactt ttaatgcatc atcatgccac
      421 atctttcttg cttcattttg catcttccat tcttctttag agatacctaa attatcaaaa
      481 tctccaattt gaggaataag attgaatgcg atctgataag ggaaggcttt tgatggaatg
      541 ttttcatctt caatttgatt ctttaaatct tcaattcctt gtacacctgc tcctgaaaca
      601 gcttgaaatg tagtcacaat cattcgtttg atgtggtatt tttcatgtaa agcttgtaag
      661 gctacaaggg caatgatagt agcacagttt ggattggcta ttaaaccgtg atgattttta
      721 atgtcttctt tattcacttc aggaatgact aaaggaacat cttgatctaa acggaatgca
      781 cttgaattat cgacaacgat acatcctgct tctttggccc atggcatcca catttttgtg
      841 atgtcattgc cagttgcacc taaaacgtaa tcaacacctt gaaagctttc ttgcgtaagt
      901 tcttcaacga tgtggccatc gatttctttt ccttttgatc ttgcactggc taataatttt
      961 agattcgctt gaatgtttct ttcttttaaa cattctaaca tttgttgtcc tacagcgcct
     1021 gtagcaccgg caattgcgat tgttgtcatt cttcatttcc tcctacaaaa gtatcatata
     1081 taacttgaac tgtttttgtg aagtcttttt tcttcactcc gactgtaata ttgatttcat
     1141 cactaccttg ggcaatcatg acgatgttaa tgttattttt acctaaagca ccaaatagtt
     1201 ttccacttgt acctggctta tcagacatgt ttttacctac agtggaaatt aaagctaagt
     1261 catcttgaac ggttaattta tctggtttta attcttcttt aaaatgagct aaaatttcat
     1321 atagactttc ttcaatttct ttcttttcta cgacaatgtt aaatgagtcg attccacttg
     1381 gaatatgttc aatactaata tggtattttt ctaaaataga tagagcttta cgaatgaagc
     1441 caacttcatt ggacatatgt tttttataga tgtagatcga catgaaatct tttttaccag
     1501 caatacccgt aattggatag ccactcttta ttttggaatg ctcctggatg atggttcctg
     1561 catcttgagg gcgattggta tttaaaatgt ggattggaat gttggcttct tttacaggga
     1621 aaatagcttc ttcatgtaaa acgctagctc ccatgtaaga aagttctctt aattctgaat
     1681 aagtgatatg tgtaattcct tttggatttt gaacgattgt gggatccgcc attagaaatc
     1741 cagatacatc ggtccagttt tcatatacgt cagcatgaag acattttgct aaaatagcac
     1801 ctgtaatatc gccaccaccg cgtgggaaga cttgaatgtg tccattggca cttgttccat
     1861 agaagcctgg gaaaacaaag tggtcatatt gggcatactt ggcttgtagt ttagcgctcg
     1921 ttgcatccca gtcgatggat ttatctaatt taaaacgaat aatatctttg gcatccacaa
     1981 aagcatagcc tagatattca gccattaatt tagcacagaa atattctccg cgacttacaa
     2041 taacagcttg cgacatttga tttaggttgg tataaaaagc atctaattct ttttcaatag
     2101 gttgcttaag gcctaattca tttttgattt cgatgaaacg atttttgatg agtttaaata
     2161 cacttgatgc atccacgtgg tattttctgt gtgcatctaa taagtataat agatcggtga
     2221 ttttgttgtc ggctgaaaat cgctttccag gagccgatac aacgatgtat cggcgtgttg
     2281 gatcttgttg aatgatgttt ttgatttttt taaattgttc tgcgtttgcg acactagaac
     2341 caccaaattt agtgattttt aacatgtgag ctacctccat aatgcgataa agctattttt
     2401 atctaattcc tttactttct taataattga catatcttga ttttcaatcc agtaataatt
     2461 ggcctccttc ttttggattg gataataatt ttctaaagca atatcacttc gaatcaacca
     2521 agtttctttt aatagagatg tatcgaattc ttttggcttt gcgtaaacaa tttttctttc
     2581 tttatcttca agtgtatcta gagcattttg taccatggct tgagctgttg gtaattgtcc
     2641 agctccctgt ccataatagc ctaatgtatc aaaactatgt gaaagaattt tttgagcgtt
     2701 gtagttgctt ggaacgttgg ctaaaaaatc attttcatcc atgcagcatg gagcgataat
     2761 ggaaataatc ttatctccaa tttgtcttga aatggataga tggcgtaata ctttgttgtt
     2821 cattttgatg aatttaatgt ctagcatttt gattgtacga attcctgcat aataaggaat
     2881 gtcaacaatt ggagtgttga aggctaaact gttagaaatc tttgtcttat agtaaacatc
     2941 aacgccatca atatcattgg atggatcttt ttctgcataa cctttttctt gagcttgttt
     3001 taaaacatct tcaaaatcta aattgttttt ttgcatagaa tctaagatat agttgctcgt
     3061 accattaaaa ataccttcat aaccttgtag tggttcaagt ctttttagtt tcaataaggc
     3121 atcaataaag ggaataccac cggcaacaca agcttctact tgaatggatc caccatattc
     3181 tttttcaagt tctaaatatg tatctaagta tgtcgcaaca accgctttgt ttgaactgat
     3241 gacatgtttg ccttttttta aagctttttg aatcagtgtg ttggctggtt ctaatccatt
     3301 taaacattca aaaacaatat caatatcatc ttgaactacc atatcttcat tgtttgaaaa
     3361 atatggaaga ttttcttttt ctttacgtac atatacgtat ttgatattga tatcttctag
     3421 tgaatttgta aggatttcaa tcccttttcc gacaactcca taacctaaga taactgcgtt
     3481 catatcgttc ctcctgtatc tttttagaaa acacttgttt ttaaagtgtg aacattgtat
     3541 catatgacat atagattttc aaggaggatt ctcatgaacg attatatttc tacaagagac
     3601 gcacaccaaa gcgtttcaag caagcaagct atactaaatg gaatgagtcc agatggaggc
     3661 ttatatgtac ttccaacttt agaccaaatt catttggatc tatctttaat atgtacaatg
     3721 tcatataaag aaaatgcagt ttatattctt tcacatcttc ttacagacta tacaaaggaa
     3781 gaattagagg catgtgttga aaatgcatat gcgaattcat tctctaaaca agaaattaca
     3841 cctattaaaa aagtagatga tgtttatgtt ttagaacttt tccacggtcc tacatgtgct
     3901 tttaaagatg ttgccctaac tcttttacct caattgatgt cctgtgcatt aaaaagcaca
     3961 agtcaaaaag ctttaatttt aactgccaca agtggagata caggtaaggc tgcattatct
     4021 ggattttgtg atgtagagaa tattggaatt aatgttttct atccatataa aaaggtaagt
     4081 gcgattcaat atcgtcagat ggttacgcaa aaaggtaaaa atgtgaatgt atttggaatt
     4141 caagggaatt ttgatgatgc gcaaagccaa gtaaaacgtt tgttcttaga tgaagaattg
     4201 aatgaatatt gtgcgaaaca aaacattttg ttgacaagcg caaattccat taatgtgggt
     4261 cgtttagttc ctcaaattgt ttattatttt gatagttata aacaattggt tctacaaggt
     4321 gatattcaat tgggggataa agtatctttc agtgttccaa caggaaattt tggagatgtt
     4381 ttagctggat attatgcgta tttaatggga cttccagtag agaaatttta tgtagctagt
     4441 aatgctaatc gtgtattaac ggatttttta acgacaggta tttatgatcg aaatcgtgat
     4501 tttatccaga ctatttcgcc aagcatggat attttgatta gttcaaattt agaaagattg
     4561 ttatattatg cttcaaataa agatacgcaa aaagtatcaa aatggatgca ggaattaaat
     4621 gaaaagggtc gttatcagat tgatgatgaa acttttaaaa ccattcaaaa tttatttgcg
     4681 tgtaacagtg ttgatgatga aaatacaagc ttagaaatt
//
LOCUS       DBMDWL010000159         4713 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7594_length_4713_cov_2.672593, whole genome shotgun sequence.
ACCESSION   DBMDWL010000159 DBMDWL010000000
VERSION     DBMDWL010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7594_length_4713_cov_2.672593"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="ACZPDN_09610"
     CDS             <1..540
                     /locus_tag="ACZPDN_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HIR1298915.1"
                     /translation="ELLTFCHTNGYKFYPTLIYCVTKVLNRIENFRMFKDKNGDLCVW
                     DSLVPNYTIFHEEDHTFSDCWTDFTDDFDSFYKNITSDMELGKTKRGIKVKLGQPANF
                     YCVSCTPWTAFTAVSSRMVNGGAAFFPIITAGKYDDNYQMPVNITIAHAVADGYHIGL
                     FFSDLQKEVSLKSLKSNLE"
     gene            629..2278
                     /locus_tag="ACZPDN_09615"
     CDS             629..2278
                     /locus_tag="ACZPDN_09615"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR1298916.1"
                     /translation="MNQSPLQKARYQYSPKLPGMLRNGISEICVKEGNATQSVADQEK
                     IAALFPNTYGEKEITFEKGENTSAAKKQVVGVILSGGQAPGGHNVICGLYDALKATSK
                     ENVLYGFKNGPIGLLEDNYVEFDDAYIDAYRNTGGFDIIGSGRTKLETEEQFAVAAKV
                     CEKHGITAIVIIGGDDSNTNAAVLAEYFAAHNTGVQVIGCPKTIDGDLKNEDIECSFG
                     FDTATKTYSELIGNIERDANSAKKYWHFVKVMGRSASHVALECALETQPNICLISEEV
                     AAKKQSLSEIADYIADAVEKRSANGNNFGVAIIPEGVVEFVPEFKALIAEINELLAGN
                     KTEEFNALGSWEEKYAFIEKGLTAESMAVFAILPQTIQQQLFLERDPHGNVQVSLIES
                     EKLFSALVKDKLTERGFTGKFNALHHFFGYEGRCAFPSNFDADYCYSLGYNAFMLIQY
                     GYNGYLSKVSNLSKPAEEWVAGGMPITKMMNIERRNGEDKPVIKKALVELDGKPFKFF
                     EANRDTWAVETAYTYPGAIQYYGPTEVCDLTTRTLALEKGE"
     gene            2324..2869
                     /gene="yfcE"
                     /locus_tag="ACZPDN_09620"
     CDS             2324..2869
                     /gene="yfcE"
                     /locus_tag="ACZPDN_09620"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR1298917.1"
                     /translation="MKYMIASDIHGSSYYCQKLLDAYKYEKPDRLILLGDILYHGPRN
                     DLPKDYAPKKVIEMLNSISDSILCVRGNCDCEVDQMVLDFPCLADYSMLDIDGLFIYC
                     THGHLKPVKLKSGSLLLCGHTHVPAIEDRDGIIYMNPGSVSIPKENSPHSYMILEDCV
                     FTWKDLETLSQYRYKEFPKSK"
     gene            complement(2829..4130)
                     /locus_tag="ACZPDN_09625"
     CDS             complement(2829..4130)
                     /locus_tag="ACZPDN_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1298918.1"
                     /translation="MNKNGNFKSTVGFVLACVGSAVGMGNIWMFPYRLGQYGGATFLI
                     PYLIFIALFGLVGLSAEFALGRMAKTGTLGAYAYCWKNKFGKYIGWLPLLGSLGIAIG
                     YSVILGWVFRSIQGVLTNELLTNDIPAFFTNMTQSFSNVPCHFLVLFVTCLLLFTSAT
                     NAIEKANKVLMPAFFILFIILAIRVSLFDGAIEGYKFLFVPKWEYLLKKETWIMAMGQ
                     AFFSLSITGSGMIVYGAYLKDDVDIPKASMQTAIFDTIAAMLAALAIMPAVFSFGIDP
                     VSGPSLMFLTLPEVFKQMPLGNFFALFFFVSVAFAGITSLINMLEAVCESWQNRFHIS
                     RKKAVLLCGIITFIISVCIENGDIVGKWMDVVTIYIIPFAALLGAITIYYVLGWNALK
                     RELDKGRKKPVGPTFKFLGKYVYVFLAIIILILGILYNGIG"
     gene            4196..>4713
                     /locus_tag="ACZPDN_09630"
     CDS             4196..>4713
                     /locus_tag="ACZPDN_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1298919.1"
                     /translation="MFVEERQNLILEDLQENGKVLVKDLSTRFGVTPDLIRKDLAFLE
                     SKGKCKKIYGGAILNRLNVHLEDANSRKNVNSKEKQKIAQMAYDLIEPNMTVFLDIST
                     TNIELAKLLVLSPISDITVVTNMLDVIQILSTSQIHSIFIGGEFDYARNGFVGNMCDE
                     FLQRFHFDLAFLG"
BASE COUNT         1639 a          756 c          880 g         1438 t
ORIGIN      
        1 gagttattaa ctttttgtca tacaaatgga tataaatttt atccaacgtt gatttattgt
       61 gtgactaaag tattgaatcg tattgaaaac tttcgcatgt ttaaagataa aaatggagat
      121 ttatgtgtgt gggattcttt agttcctaac tatacaatct tccacgaaga agatcataca
      181 ttttcggatt gttggaccga ttttacggat gattttgatt ctttctacaa aaatattact
      241 tctgatatgg aattaggtaa aacaaaaagg ggaatcaagg tgaaattagg ccaacctgca
      301 aacttttatt gtgtttcatg tacgccctgg actgcattta cagcggtttc aagtcgtatg
      361 gtcaatgggg gtgctgcctt ctttccaatc atcacagctg gaaaatatga tgataattat
      421 cagatgcctg taaatatcac aattgcgcat gcggttgcgg atggatatca tattggcttg
      481 tttttttctg atttgcaaaa agaagtaagt ttaaaaagct tgaaatcaaa ccttgaataa
      541 aggccttgga tggggtgctc tatttacttt ttgggcaaag tgttataaaa tttttaatgt
      601 aggtataaat tgaaaaggag atataaaaat gaatcaatca ccacttcaaa aagctagata
      661 ccaatacagt ccaaaattgc ctggaatgtt aagaaatggt atttcagaaa tctgtgttaa
      721 ggaaggaaac gcaactcaaa gtgttgcaga ccaagaaaaa atcgcggctt tattccctaa
      781 cacttacgga gaaaaagaaa ttacattcga aaaaggagaa aatacttctg cagctaaaaa
      841 acaagttgta ggtgtaatct tatctggtgg acaagcacct ggtggacaca acgttatttg
      901 tggtctatat gatgcattaa aagctactag taaagaaaac gttctttacg gatttaaaaa
      961 tggtcctatt ggattattag aagacaacta tgtagaattc gatgatgctt atatcgatgc
     1021 atacagaaat actggtggat ttgatattat cggatctgga cgtacaaaat tagaaactga
     1081 agaacaattc gcagttgctg caaaagtttg tgaaaaacac ggaatcactg ctatcgtaat
     1141 tatcggtggt gatgactcaa atacaaatgc tgctgtatta gctgaatact ttgctgctca
     1201 caatacaggt gttcaagtta tcggttgtcc aaaaactatc gatggtgact tgaaaaacga
     1261 agacatcgaa tgttcattcg gattcgatac tgcaactaaa acatatagtg aattaatcgg
     1321 aaacatcgaa cgtgatgcaa actcagctaa aaaatattgg cactttgtaa aagttatggg
     1381 acgtagtgca agtcacgtag ctttggaatg tgctttagaa actcaaccaa acatttgttt
     1441 aatttcagaa gaagttgctg ctaaaaaaca atctttatct gaaatcgctg actatattgc
     1501 agatgctgtt gaaaaacgtt ctgcaaatgg aaataacttc ggtgttgcaa tcatccctga
     1561 aggtgtagtt gaattcgttc cagaatttaa agcattgatt gctgaaatca acgaattatt
     1621 agctggaaac aaaacagaag aatttaatgc attaggtagc tgggaagaaa aatatgcatt
     1681 cattgaaaaa ggtttaactg ctgaatcaat ggctgtattc gcaatcttac ctcaaactat
     1741 ccaacaacaa ttattcttag aaagagatcc acacggaaac gtacaggtat ctttaattga
     1801 atcagaaaaa ttgttctcag ctttagttaa agataaatta actgaaagag gatttacagg
     1861 taaattcaat gcattacacc acttcttcgg atacgaagga cgttgtgcat tcccatcaaa
     1921 ctttgatgct gactactgct actcattagg atacaacgca ttcatgttga tccaatacgg
     1981 atataatgga tatttatcaa aagtatctaa cttatctaaa ccagctgaag aatgggttgc
     2041 tggtggtatg ccaatcacta agatgatgaa tatcgaacgt cgtaatggtg aagataaacc
     2101 agttatcaaa aaagcattgg ttgaattaga tggaaaacca ttcaaattct tcgaagcaaa
     2161 ccgtgataca tgggctgttg aaactgcata tacttatcca ggagctatcc aatattatgg
     2221 accaactgaa gtatgtgatt taacaactcg tacattagct ctagaaaaag gtgaataata
     2281 aataaaagaa tgcttcggca ttcttttttt aaaggagaaa tttatgaagt atatgatcgc
     2341 aagtgacatc catggttcaa gttattattg tcaaaaacta ttggatgctt acaaatacga
     2401 aaaaccagat cgattgattt tattggggga tattttatat catggcccta gaaatgatct
     2461 gccaaaagat tatgcaccaa aaaaagtgat tgagatgttg aactcgattt cagactcgat
     2521 tttatgtgtt cgtggaaatt gtgattgcga agttgatcaa atggtattgg attttccatg
     2581 tttggcagat tattcgatgt tggatataga cgggctcttt atttattgta cacacggaca
     2641 tttaaaacct gttaagctaa aatctggatc tttattacta tgtggacata cacatgtgcc
     2701 tgcaattgaa gatcgagatg gtattattta tatgaatcca ggctcagttt cgatcccaaa
     2761 agaaaacagc cctcatagtt atatgatact agaggactgt gtatttactt ggaaagattt
     2821 agaaacgctt agccaatacc gttataaaga attcccaaaa tcaaaataat aatggcaaga
     2881 aatacgtaaa catatttacc taagaacttg aaagtcggtc ctaccggctt ttttcttcct
     2941 ttgtctaatt ctcgttttaa agcattccac cctaaaacat agtaaatggt aatggctcca
     3001 agtagtgctg cgaaaggtat aatatagatt gtgacaacat ccatccattt acctacgata
     3061 tctccatttt caatacatac agaaatgatg aatgtgataa ttccacaaag taaaaccgct
     3121 ttttttctag aaatgtgaaa gcgattttgc caagattcac acacagcttc taacatgttg
     3181 attagactgg tgattccggc aaaggctact gaaacaaaga agaatagggc aaagaaatta
     3241 ccgagtggca tttgtttaaa tacttcagga agagttaaaa acattaaact aggaccacta
     3301 acggggtcaa tgccaaaaga gaatacggca ggcataatcg caagtgctgc aagcatcgca
     3361 gcaattgtat caaatatagc cgtctgcata cttgcctttg gaatatctac atcatccttt
     3421 aaataagctc catatacaat cattccagaa ccggtaatgg ataaggagaa aaacgcttgt
     3481 cccatagcca tgatccatgt ttcttttttg agtaaatatt cccatttagg aacaaataaa
     3541 aatttatatc cttctatagc accatcaaat agactgacac gaatggcaag gataataaat
     3601 aaaataaaga aggctggcat taatacttta ttggcctttt caatggcgtt tgtggcactt
     3661 gtaaaaagaa gtaaacaagt gacaaacaag actaaaaagt gacatggcac attcgaaaag
     3721 gattgcgtca tatttgtaaa gaaagctgga atatcatttg tgagtagttc gtttgtgagt
     3781 acaccttgaa tactacgaaa tacccatcct aaaatgacac tatatccaat cgcaatccca
     3841 agagatccaa gtagcggaag ccatccaata tatttaccaa acttattttt ccaacaatat
     3901 gcataagcac ctaacgttcc tgtttttgcc attcttccta gcgcaaattc agccgataaa
     3961 ccaacaagac caaataaggc gataaaaatt aaatatggta ttagaaaggt agctcctcca
     4021 tattgtccta agcgatatgg aaacatccaa atgtttccca ttcctacggc agaacctacg
     4081 caggcaagca caaatcctac cgtactttta aaatttccgt ttttattcat ttgtagtacc
     4141 tcgatgtttc ttagtatatc acaaaaatga tataatattt aaacaggtga tgagtatgtt
     4201 tgtagaagaa agacaaaatt taattttaga agatttacaa gagaacggta aagtgttggt
     4261 aaaagatttg agtacacgtt ttggtgtaac ccccgattta attcgtaaag atttagcttt
     4321 tttagaatcg aaagggaaat gtaaaaagat ttatggtgga gcgattttga atcgtttaaa
     4381 tgtgcattta gaggatgcca attctagaaa gaatgtgaat agtaaggaga aacaaaaaat
     4441 agcgcaaatg gcttatgatt tgattgaacc aaatatgacc gtctttttgg atatttcgac
     4501 tacgaatatt gaattagcta aattattagt cttaagtcct atttcagata ttactgtcgt
     4561 taccaatatg ttggatgtaa ttcaaattct ttctacaagt caaatccatt ctattttcat
     4621 tggtggagaa tttgattatg caagaaatgg ctttgtgggt aatatgtgtg atgaatttct
     4681 tcaacgtttt cattttgatt tagctttttt agg
//
LOCUS       DBMDWL010000160         4643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7770_length_4643_cov_2.448481, whole genome shotgun sequence.
ACCESSION   DBMDWL010000160 DBMDWL010000000
VERSION     DBMDWL010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7770_length_4643_cov_2.448481"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="ACZPDN_09635"
     CDS             <1..302
                     /locus_tag="ACZPDN_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024258.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1298920.1"
                     /translation="QRLTVLDKRIHFSHGDEGLDEIKKDIDQHHPDIYLYGHTHLVKE
                     QIYNNCLILNPGSITLPRDDWHGSYIILTIDKKVTYKVVRINVETFLNQYQCFIE"
     gene            complement(338..1339)
                     /gene="galE"
                     /locus_tag="ACZPDN_09640"
     CDS             complement(338..1339)
                     /gene="galE"
                     /locus_tag="ACZPDN_09640"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1298921.1"
                     /translation="MNILVTGGTGYIGSHICVELLESGYDVVVIDDFSNSKPDVLDSI
                     YKITGKHVKFYEFNVLDEEKTEAVFKENKLDAVIHCAAFKAVGESVQKPIEYYTNNLM
                     TTLVVAKMMKKYHVPSIVFSSSATVYGDPEVVPLTEDCKLGETTNPYGSTKAMMERIL
                     TDVQFANPEMSVTLLRYFNPIGAHESGLIGENPKGIPNNLMPYIMKVATGELSELGVF
                     GNDYDTPDGTGVRDYIHVVDLAKGHVLAIEKYNTPGVHICNLGTGKGYSVLDIVHAFE
                     RVNGIKIPYVIKPRRAGDIATCFADPTRAKVQLGWVAEKNLDDMCRDTWNFAKQFIK"
     gene            complement(1339..2619)
                     /locus_tag="ACZPDN_09645"
     CDS             complement(1339..2619)
                     /locus_tag="ACZPDN_09645"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1298922.1"
                     /translation="MKTTEIKEQVVSGKYDALIEDLYADPSLLDYQKQRYAAALDKYQ
                     TLFGDDEVSIYSAAGRSEISGNHTDHQHGCVLAGSINLDAIGIVSKQDHVIKVISDDF
                     DIKPIDLNDLDKKEDELGTSEALIRGVVYKLKELGYNVGGFKAFITSDVLMGAGLSSS
                     AAFESIIGTIIDGLYNDMKIDMVTIAKVGQYAENVYFGKPCGLMDQCACAVGGLISID
                     FKDTSNPIVNSVNVDFSKYDHSLCIVDTKGSHADLTDAYGAVPQEMKEVAHYFGKEVL
                     REVDEDEFYANIANLRTALNNDRAILRAIHFFNENRRVNTIVERLNKDDFEGFKTLIQ
                     ESGNSSYKFLQNVYADFDYKNQAVSLGLAMSEMILKDHGVCRVHGGGFAGTIQAFVEN
                     SYVETYKEEIEKVFGKGSCHILKVRKLGGCKVID"
     gene            complement(2616..3629)
                     /locus_tag="ACZPDN_09650"
     CDS             complement(2616..3629)
                     /locus_tag="ACZPDN_09650"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1298923.1"
                     /translation="MVEVKERLENGIDVLSISNSHLTVEVTNFGCTLLKIVAKDKNNQ
                     DCDCILGFENVSDYQKRDGTYLNALVGRVCNRIEKGTFTINDTEYHVPINNGPNSLHG
                     GIEGFSYKVFDYSLIEDGVKFHYVSKDGEEGYPGTLDFYAIYWLDGPSLHIQYSGVSD
                     KDTLINITNHGYFNLNGYASNIDDHTLRILASKFGCVDPDGLYTGELKDVKDTAFDFN
                     TEKRIGDSIHAEDEQLIIARGYDHPYVFDTKENQVCLYSPLSGIELKVSTTYPTAQIY
                     SANYLDGQTDKYGKIMHPQDGLCIETSYIADSIHKEENPKVLLKAGQVFEEETTYTFE
                     VRK"
     gene            complement(3623..>4643)
                     /locus_tag="ACZPDN_09655"
     CDS             complement(3623..>4643)
                     /locus_tag="ACZPDN_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1298924.1"
                     /translation="PEKDPKEIAKAKNAVSTSYPACLLCKENVGFAGNLNHPARQTHR
                     IIPLDLNGHTYNLQYSPYVYYNEHCIVFNSEHTPMKMDRSTFEHLFAFIDKFPHYMIG
                     SNADLPIVGGSILSHDHYQGGRHHFPMEDAKVVKSYTYKDVQVDMLYWPLSTLRLTSE
                     SKENVLDLATKIFETWCTYNDESLDIIANEDGVRHNTVTPIVRKKDGKYQMDVVLRNN
                     RTTEEYPLGIFHPHSEHHHIKKENIGLIEVMGLAILPARLKTELEEIKLCLSGKADFD
                     SYPELEKHKDWYEHLKTCSYTNVDTFMEMEVTKKFVSVLENAGVYKMDDEGMDGFSRF
                     VKTLW"
BASE COUNT         1425 a          966 c          624 g         1628 t
ORIGIN      
        1 accaaagatt aactgtttta gacaaacgca tccatttctc acatggagat gaaggtttgg
       61 atgaaataaa aaaagatatc gatcaacatc atcccgatat ctatctttat ggacatacac
      121 atttagtcaa agaacaaatc tacaacaatt gcttgatttt aaatccaggt tcaatcactt
      181 tacctagaga tgattggcat ggatcttata tcattctaac aatcgataag aaagtcacat
      241 ataaagtggt tcgaatcaat gtagaaactt tcttaaatca atatcaatgc ttcattgaat
      301 aaggcagcca aatcattggc tgcctgtttt ctttaattta ttttataaac tgttttgcga
      361 aattccatgt atctctacac atatcatcta aattcttttc agctacccaa ccaagctgaa
      421 cctttgctct tgtaggatct gcgaaacatg tcgcaatatc tcctgcacgt ctaggtttaa
      481 tgacataagg aatcttaata ccattcactc tttcaaacgc atgtacaata tctaatacac
      541 tatacccttt tcctgttccc aagttacaaa tatgaacacc aggcgtattg tatttttcaa
      601 tcgctaatac atgtccttta gccaagtcga ctacatgaat atagtcacga acacccgttc
      661 catctggcgt atcataatca ttgccaaata caccaagttc actaagttct ccagtcgcaa
      721 ccttcataat atatggcatc aagttatttg ggattccctt tggattttct ccaatcaaac
      781 ccgactcatg cgctccaatt ggattgaaat aacgaagtaa cgttacactc atttcaggat
      841 ttgcgaactg cacatccgtt aaaattcttt ccatcatcgc ttttgttgaa ccatatggat
      901 ttgtcgtttc accaagctta caatcttctg ttaaaggaac cacttcagga tctccataaa
      961 ctgttgcact tgaactaaat acaatactag gcacatgata ctttttcatc atcttggcaa
     1021 ccactaaagt agtcatcaag ttatttgtat aatactcaat tggtttttga acactttcac
     1081 caaccgcttt aaaagccgca caatgaatga ccgcatccaa tttattttcc ttaaatacag
     1141 cttccgtctt ttcctcatct aacacattaa attcataaaa cttaacatgt tttcctgtaa
     1201 tcttatagat tgaatccaat acatctggtt ttgaattgga gaaatcatcg atcacgacaa
     1261 cgtcataacc actctctaac aattcaacac aaatatgact accaatatat ccggtaccac
     1321 ctgttactaa aatattcatt agtcaataac cttgcagcca cctaattttc ttacttttaa
     1381 gatatggcag cttccctttc cgaatacttt ttcaatttct tctttatatg tttctacata
     1441 gctgttttca acaaaagcct gaatcgttcc ggcaaaacct ccaccatgaa cacgacatac
     1501 tccatgatct tttaaaatca tttcagacat agctaaacct aaagatacag cctggttctt
     1561 atagtcaaag tctgcatata cgttttgaag gaacttgtag cttgaatttc ctgattcttg
     1621 aatcaatgtt ttgaagcctt caaaatcatc cttattcaat cgttctacga ttgtattgac
     1681 acgtctattt tcattgaaga aatgaatcgc tcttaagata gcacgatcat tatttaaagc
     1741 cgttcttaag tttgcgatat ttgcatagaa ctcatcttca tccacttcac gaagaacttc
     1801 cttaccaaaa tagtgagcta cctctttcat ttcttgtggt actgcaccat aagcatctgt
     1861 taaatcggca tgacttccct ttgtatctac aatgcataaa ctgtgatcat attttgagaa
     1921 atcaacattc acactattta caattggatt tgaagtatct ttaaagtcaa ttgaaataag
     1981 cccaccaaca gcacatgcac attgatccat caatccacat ggttttccaa agtatacgtt
     2041 ttcagcatat tgacctactt tagcgatcgt taccatatcg atcttcatat cattgtataa
     2101 tccatcaata attgtaccaa taatagattc aaatgctgca gagcttgata aaccagcccc
     2161 cattaataca tcactcgtaa taaaagcttt aaatccacca acgttatagc ctaattcttt
     2221 taatttatag acaacaccac gaatcaaggc ttcacttgtt cctagttcat cttctttttt
     2281 gtctaaatca tttaaatcaa ttggtttgat gtcaaagtca tctgaaatca ctttaatgac
     2341 atgatcttgt ttagatacaa ttccaatcgc atctaaattg atagatcctg caagtacaca
     2401 accatgttgg tggtctgtat gatttcctga aatttcactt cttcccgccg cactataaat
     2461 actcacttca tcatctccaa ataaagtttg gtatttatct aatgcagctg cataacgttg
     2521 tttttggtag tccaataaac ttggatctgc atatagatct tcaattaatg catcatattt
     2581 tcctgatacg acttgttctt taatttctgt tgttttcatt ttcttacctc gaatgtatat
     2641 gttgtttctt cttcaaatac ttgtcctgct tttaataaaa cttttggatt ctcttcttta
     2701 tgaatcgaat cggcaatata gcttgtttca atacacaatc catcttgtgg atgcataatc
     2761 tttccatatt tatctgtctg tccatctaag tagttcgcac tataaatttg tgccgttgga
     2821 tatgttgtgg atacttttaa ttcaatacca cttaatggac tatataaaca tacttgattt
     2881 tcttttgtat caaatacata tggatgatca tatcctcttg cgatgattaa ttgttcatcc
     2941 tctgcatgaa tagaatctcc aattcttttt tctgtattaa aatcaaatgc cgtatccttc
     3001 acatctttta attcacctgt atataatcca tctggatcta cacaaccaaa tttagatgcc
     3061 aaaattcgta acgtatgatc atcaatattt gatgcatagc cattcaaatt gaaataacca
     3121 tgatttgtga tattgatcaa agtatccttg tcactgactc cactatattg aatatgaaga
     3181 cttggtccat ccaaccaata gatcgcatag aaatctaatg ttccaggata gccttcttct
     3241 ccatcttttg atacatagtg gaatttaacg ccatcttcaa tcaaagaata atcaaatact
     3301 ttataagaga atccctcaat acctccatgt agtgagtttg gtccattatt aataggtaca
     3361 tgatattctg tatcattaat cgtgaatgta cctttttcaa tacgattaca aacgcgtcct
     3421 actaatgcat ttagataggt tccatctctt ttttgatagt cacttacatt ttcaaatcct
     3481 aaaatacaat cacagtcttg attattctta tctttcgcta caatctttaa aagagtacaa
     3541 ccaaagtttg taacttctac agttaaatgt gaattggaaa tcgataatac atcaattcca
     3601 ttctctaatc tttcttttac ctctaccata atgtcttcac aaatcggcta aagccgtcca
     3661 tcccttcatc atccatttta tatacgccgg cattctctaa tacagatacg aatttctttg
     3721 tgacttccat ctccatgaat gtatccacat ttgtataaga gcatgttttt aggtgttcat
     3781 accagtcttt atgtttttca agttctggat aagaatcaaa atccgctttt ccagataaac
     3841 ataatttaat ttcttctaac tccgtcttta atcgtgctgg caaaatcgca agtcccataa
     3901 cttcaatcaa accaatattt tcttttttaa tatggtgatg ctctgagtga ggatggaaga
     3961 tacctaatgg atattcttct gtcgtacgat tgttacgaag tactacatcc atttgatact
     4021 tgccatcttt ctttcttaca attggcgtta ctgtattgtg acgaacacca tcttcattgg
     4081 cgatgatatc taaactttca tcattatatg tacaccatgt ttcaaagatt ttagtagcta
     4141 gatccaatac gttttctttc gattcagatg ttaatcgaag tgttgatagt ggccagtaaa
     4201 gcatatccac ttgtacatcc ttatatgtat agctcttaac aaccttggcg tcttccattg
     4261 gaaaatggtg tcttccacct tgatagtgat catgacttaa aatcgatcca ccaacgatag
     4321 gaaggtctgc attacttcca atcatatagt gcgggaactt atcaataaat gcgaataaat
     4381 gctcaaatgt agaacgatcc atcttcattg gtgtatgttc actattaaat acgatacagt
     4441 gttcgttata atacacatat ggtgaatatt gtaaattgta tgtatgtcca tttaaatcta
     4501 atggaataat acgatgtgtt tgtctagctg gatgatttaa gtttccggca aaaccaacat
     4561 tttccttaca taataaacat gcaggatatg aagtgcttac ggcattttta gctttagcaa
     4621 tttcctttgg atctttttct ggt
//
LOCUS       DBMDWL010000161         4614 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7844_length_4614_cov_1.948815, whole genome shotgun sequence.
ACCESSION   DBMDWL010000161 DBMDWL010000000
VERSION     DBMDWL010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7844_length_4614_cov_1.948815"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(135..323)
                     /locus_tag="ACZPDN_09660"
     CDS             complement(135..323)
                     /locus_tag="ACZPDN_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298925.1"
                     /translation="MGRPRTNNVQINIAIPGEWKKQLENLARIYSVEEGKTITFLDLM
                     RRGIQEKYQLKDVNDHEE"
     gene            complement(659..2398)
                     /locus_tag="ACZPDN_09665"
     CDS             complement(659..2398)
                     /locus_tag="ACZPDN_09665"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298926.1"
                     /translation="MFEILAKFFKFSGEENSKKFKLSIGIGLVEALASAMKIPAIMYI
                     LMGLIEEKSMGKYIFGSLVIMIIAIVVDVICKRISSVLQTEGGYNASAFMRIKIAEHL
                     RYLPMGYFNSNSIGEIASVTTNTMEVLGDVASRVVMLTMQGILETTMIICMILIFDVR
                     IGLISVVGVIVFFIINSMMQKAGKKDSEQKVQCDTELVNQIMEYVQGISEVKSYNLLG
                     KQAKRLNDANDACAKINTKMELVFVPYHFLQGIVTKITGTIIIICCAAFYINGTMNSV
                     YAIGMSISAFILYSSLETAGNYSSLLHVVSVCVDKANDILALNTMDIEGREIESKSHD
                     IQLDHVSFSYDKRKIIDDVSLSIPEKTTTAIVGPSGGGKSTLCNLIARFWDVDEGEVS
                     LGGINVKDYSMNSLMNNFAFVFQRVYLFADTIENNIKFGNQEATHEQVVMAAKKACCH
                     EFISQLPDGYNTVIGEGGATLSGGEKQRISIARAIMKDAPIIILDEATANVDPENEKE
                     LIEAIDALTKEKTIIMIAHRLKTVRNADQIVVIDKGRIVQQGTHNQLMKQDGIYKRFV
                     DSREQAVSWKLAH"
     gene            complement(2400..4154)
                     /locus_tag="ACZPDN_09670"
     CDS             complement(2400..4154)
                     /locus_tag="ACZPDN_09670"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1298927.1"
                     /translation="MEEKKKSTMRWILEWAGQKKMDYVWSVILGMGNVIFKIIPYFLI
                     ATIVRMFLEGNKDFNTYLKNSICITISFIVAELCHSLSTTLSHKATFTVLSNIRKACF
                     EKLARVPLGYVKDTSSGTFKNIMVERIDSIETTLAHIIPEFTSNLLGPIAILIYFFTI
                     DYRLALWSLLPIIIGFLSYFGMMIDFKPNFEKTVNATKDLNDAAVEYIDGIEVIKAFG
                     KTESSYAKFTSAAKAYANSFISWMKKCSIYHALMMTITPYTLLTVLPFGVHYVENGTL
                     TMPDFILCIILSLGIAGPLITAFSYTDDLGKIGVIVDEVVGILDQPELSRPYKSMAVP
                     KDNSIELSDVKFGYHDKEILHGIHMECKAGSVNAIVGPSGSGKSTIAKLIAALWDVNS
                     GSIKIGGVDIKQMSLMDFNQKIAYVAQDNYLFNETVRENIRQGNPNASDEDIIEVTKK
                     SGCYDFIMQLENGFDTIVGGAGGHLSGGERQRISIARAMLKDAPIVILDEATAYTDPE
                     NEAILQNSIARLVEGKTLIVIAHRLSTIKDSDQIFVVNDGNVVAHGKHEQLLLSCSLY
                     KDMWNAHISVKDTVKEGK"
     gene            complement(4222..>4614)
                     /locus_tag="ACZPDN_09675"
     CDS             complement(4222..>4614)
                     /locus_tag="ACZPDN_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1298928.1"
                     /translation="KLSAYDQTNNMSNVATDGTDQMIDYIYKHYDHFRLLFKCGDSGK
                     YEAFIHNMVNKEMKATKKYMQIMKEAGINLLNVDESLMHMIYTGFFSSILQIVEHDMD
                     KEIAKKNVHQLKEFNTGGWERIWNIQFK"
BASE COUNT         1506 a          964 c          582 g         1562 t
ORIGIN      
        1 tatatccata cgcatcacaa acttcagaaa gaatcttctt caaggtttca tccataccat
       61 cacatctgaa ctttggacac catataatat gatactgaac taaatactta caatgagaag
      121 cactatgata attctcattc ttcatgatca ttcacatctt ttaactgata cttttcctga
      181 atgcctctac gcatcaaatc tagaaaagtg atcgttttac cttcttcaac cgaatagatt
      241 ctggcaaggt tttccagttg ttttttccat tcaccaggaa tagcaatatt gatctgaaca
      301 ttattcgttc taggtctacc catgatatca acccccaact atttctacat atattatata
      361 tttttcatac gcattataca atacaaaaag tggctttcat ctttacagca agctatggag
      421 ttttcgccac ttatttataa catattaaag tgcgtttaag ggtactgttt ttttaatatt
      481 tttaaaaaca gtacccttaa acgccattta tctttttgct tgtattttct tagcatctac
      541 aaaaagaatc taccaatcaa aataaagaaa acaagcaata atgaacagag ttaaactatt
      601 caacacaata aaatagcgta atcatttttg gactacgcta tctgttttta ttactaaatt
      661 aatgtgcaag tttccaactt actgcctgct ctctagaatc cacaaaacgt ttataaatac
      721 catcttgttt cattaattga ttgtgtgttc cttgttgtac aattcttccc ttatctatca
      781 caactatttg atccgcgttt cggacggttt ttaatctatg tgcaatcata ataattgtct
      841 tttcttttgt caaagcatcg atagcttcta ttaattcctt ttcattttct ggatccacat
      901 tggctgttgc ctcatccaaa ataataatgg gtgcatcctt cataatggca cgtgcaatag
      961 aaatgcgttg tttttcaccg ccagaaagag ttgctccacc ttcaccaata actgtattat
     1021 atccatctgg caattgactt ataaactcat ggcaacaagc tttcttagct gccattacaa
     1081 cttgttcatg agtcgcttct tgattgccaa atttaatatt attttcaatg gtatccgcaa
     1141 acaagtacac tctttgaaat acaaacgcaa agttattcat caaactattc atactataat
     1201 cctttacatt tataccccca agactcactt caccttcatc tacatcccaa aatctcgcaa
     1261 tcaaattgca caatgtagat ttacctcctc cagaaggacc tacaatcgct gtagttgttt
     1321 tttctggaat cgacaaggat acatcatcaa tgatttttcg cttatcataa gaaaatgaaa
     1381 catgatccaa ttgaatatca tgactcttgg attctatttc ccttccttca atatccatcg
     1441 tattcaatgc aagaatatca ttggctttat ctacacagac acttaccaca tgtaaaaggg
     1501 atgaataatt tccagctgtt tctaaacttg aataaagtat aaaagccgaa atactcatac
     1561 caatcgcata caccgaattc attgttccat taatataaaa tgcagcacaa caaataatga
     1621 tgattgtccc agtaatcttt gtcacaatac cctgaagaaa atgataaggt acaaatacaa
     1681 gttccatttt tgtattgatt ttagcacatg catcgttggc atcattcaat cgtttcgcct
     1741 gtttacccaa taagttataa gattttactt ccgaaatacc ttgcacatat tccatgattt
     1801 gattcacgag ttctgtatca cactgcacct tttgttctga atctttttta ccagcttttt
     1861 gcatcataga attaattata aagaatacaa taacaccaac aacagatatc aatccaatcc
     1921 ttacatcaaa aataaggatc atacatataa tcattgtagt ttctaaaatt ccctgcatcg
     1981 taagcattac cactcttgat gcaacatctc caagtacttc cattgtattt gtcgtaacag
     2041 aagcaatctc accaatacta tttgaattaa aatatcccat tggaagataa cgaaggtgtt
     2101 ctgcaatctt aattcgcata aatgcactcg cattatatcc accttctgtc tgaagtacag
     2161 acgaaattct tttacaaata acatccacca caatcgcaat aatcataatg actaatgagc
     2221 caaaaatata tttacccatt gatttttctt caatcaagcc catcaatata tacataatag
     2281 ccggtatttt catagctgaa gcaagtgctt ctactaagcc aattcctatt gataatttaa
     2341 atttcttact gttttcttca ccagaaaatt taaagaattt tgccaagatt tcaaacatat
     2401 tattttccct cctttactgt atccttcaca gaaatgtgtg cattccacat atctttataa
     2461 agtgaacaag acaaaagaag ttgttcatgt tttccatgtg ctactacatt tccatcattt
     2521 accacaaaga tttgatcact gtcttttatt gtagaaagcc tatgtgcaat cacaatcaat
     2581 gtttttcctt ctacaagtcg tgcaatagaa ttttgaagaa ttgcttcatt ttcaggatcc
     2641 gtatacgctg tagcctcatc taaaatcaca ataggtgcat cttttaacat ggctctagcg
     2701 atagaaatac gttgtctttc tcctccagaa agatgacctc ctgcaccacc gacaatcgta
     2761 tcaaagccat tctctaactg cataataaag tcgtaacaac cactcttttt tgtgacttca
     2821 attatatctt catcactcgc attgggattt ccttgacgga tattttcgcg tacagtttca
     2881 ttaaacaaat aattatcctg tgctacatat gcgatttttt gattaaagtc cataagagac
     2941 atttgtttga tatctacacc accaattttt atacttccag aattcacatc ccataaagcc
     3001 gcaatcaact ttgcgatggt tgacttacca cttccagacg gacccacaat cgcattcaca
     3061 cttccagcct tacactccat atgaatgcca tgaagaattt ctttatcgtg atatccaaac
     3121 ttaacatctg aaagttcgat cgaattatct tttggcacag ccatactttt atatggccta
     3181 cttaactctg gctgatctag aattccaaca acctcgtcaa caatgacacc tatctttccc
     3241 aaatcatccg tataagaaaa ggctgtgatc aatggtccgg caatccctaa tgataaaatt
     3301 atgcataaga taaaatccgg cattgtaagt gtgccatttt caacataatg aactccaaaa
     3361 ggaagcacag tcaataatgt atagggagtt attgtcatca tgagtgcatg ataaatagaa
     3421 cattttttca tccaggaaat aaaagaattg gcataggcct tggcagcact cgtaaatttc
     3481 gcataagaac tctctgtttt tccaaaagct ttaatgactt cgattccatc aatatattca
     3541 acggccgcat catttaaatc tttcgttgca tttactgttt tttcaaagtt tggtttaaaa
     3601 tcaatcatca ttccaaaata agataagaag ccaataataa tgggtaataa tgaccaaaga
     3661 gccaatctat aatcaattgt aaaaaaataa atcaatatag caataggacc aagtagattt
     3721 gaagtgaatt caggaataat atgtgccagc gttgtttcaa tactatcaat tctttccacc
     3781 ataatattct taaatgtacc tgatgaagta tctttcacat atcctaatgg cactcgtgct
     3841 agcttttcaa aacatgcctt acgaatgttt gaaagcacag taaatgttgc cttatgcgaa
     3901 agagttgttg agagcgaatg acaaagctcg gccacaataa aggaaatagt tatacatatt
     3961 gaatttttta gatatgtatt aaaatcttta ttcccctcta aaaacatcct tacaatcgtt
     4021 gcgataagaa aataaggaat gattttaaaa atcacattac ccattccaag tatcacactc
     4081 catacataat ccattttctt ttgtccagcc cactctagta tccagcgcat tgtagatttc
     4141 tttttttctt ccatacaaat ccctcctaat tattagtcat aactaactgc attgttataa
     4201 gtataggtgg caattgccac cttatttaaa ttgaatattc caaattcttt cccaaccacc
     4261 tgtattaaat tcttttaatt gatgaacatt tttcttagct atctctttat ccatatcgtg
     4321 ttcaacaatt tgcagtatag acgagaaaaa gcctgtgtaa atcatatgca taagactttc
     4381 atcaacattt aataaattta ttccagcctc tttcataatc tgcatatatt tcttggttgc
     4441 cttcatttct ttattcacca tattatgaat aaaagcttca tatttcccac tatctccaca
     4501 tttaaacaat aatctaaaat gatcataatg cttataaata tagtcaatca tttgatctgt
     4561 tccatcagta gctacatttg acatattgtt tgtctgatca taagcagaaa gctt
//
LOCUS       DBMDWL010000162         4610 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7857_length_4610_cov_2.063650, whole genome shotgun sequence.
ACCESSION   DBMDWL010000162 DBMDWL010000000
VERSION     DBMDWL010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7857_length_4610_cov_2.063650"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /locus_tag="ACZPDN_09680"
     CDS             <1..2062
                     /locus_tag="ACZPDN_09680"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1298929.1"
                     /translation="KSLKRARQTISVDQVISNRILILAVSITVCFLIIFLRLFYLQIV
                     SYNDYTAKREDYTSIKQYTSAPRGQIYDCKGRILAKTVVSHNIVFTSPNNMDSDDYEL
                     YAKRIVKVFRVSAKDFTEEDKKQAYITYKSFLSVNDSEYAANHLLTSKELKQYKNGAW
                     GDNAESKRYQILMNRIGKKQIGEMTQSELKMCVIYQRMIANQSTGQENVVLEDVSDDD
                     VAYLVEHKTEFPGFDVDFGGWKREYPYGESLSDVLGSVTTSTQGLPSELSSYYLSKGY
                     QLNASVGKSGLEYQYNDLLAGTPEIAKITYNSKGLAQKEVVQEAKKGYDIHLSIDIDL
                     QTSLDDILKKTLNEYGGSANRENFKSLFTTVLNAKDGSVLAMSGYQMDLDTKGMTYFS
                     TGNYMSLVNPGSCIKGATVYMGESEHVMSPGETIVDKTMNIGGQEFSSYKDYGTVDDI
                     MALEVSSNIYMFNVAIRLANASYQEEQPLYVNDLQGTLNKMRGYYSMFGLGNKTGIDA
                     PNEASGYMGYGAEPGMLLYYSIGQFDMYSPLQLATYGATIASNGNLYKPRFMSYATEV
                     NSQEVVVTNAKKIKSKLPEKNAAYLDRVKQGFSACVSSGYCGDPMQPFAGKVSAKTGT
                     AEVGDWTTANLVGYAPSDDATMAFACSAPTSSINDQSVSPNICSNMVMPEFIQKYFEL
                     YPAS"
     gene            2142..2291
                     /gene="rpmG"
                     /locus_tag="ACZPDN_09685"
     CDS             2142..2291
                     /gene="rpmG"
                     /locus_tag="ACZPDN_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1298930.1"
                     /translation="MRDRITFKCSVCGEENYIGTRNKKKHPERMTVKKYCPKCNQQTE
                     HKEKK"
     gene            2331..2954
                     /locus_tag="ACZPDN_09690"
     CDS             2331..2954
                     /locus_tag="ACZPDN_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1298931.1"
                     /translation="MKVIQQPLGPVQANCYLVMENHHALIIDPADILPNLDEILNQND
                     CTLDAIVLTHAHFDHISGVDKIVNKFACDVYLNPNEFDFLLDPDLNSSSAFYMDVTCN
                     ANAKPLIEGKNEIAGFEVEAIYCPGHSIGSTVLKIEDCLFTGDVLFQGSIGRMDLATG
                     SVSSMKQSLKKLVQFNKDYKVYPGHGPTTSLSQEKKWNESLKYAEIY"
     gene            2999..3988
                     /locus_tag="ACZPDN_09695"
     CDS             2999..3988
                     /locus_tag="ACZPDN_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR1298932.1"
                     /translation="MEDLLKDLIEYAIDKKASDIHFVLHNQRLQIQLRTQNEMIDVYQ
                     DIWKPSFFEYLKFISQMDLTNPYTPQSGQFKVYLHNHDIFCRFSILHNHNIQTGVIRL
                     LNTKQAIQLYELSLWKKDTQFFQSLIPLRQGLVLCVGPTNSGKTTTLHALLHEIAKTR
                     KHKVVSLEDPIEIEDDSYLQLQINETMGFTYDVGIEQLLRHDPDIICIGETRNSYTAK
                     MVLRASLTGHLVLTSIHAKNGKECISRLEDLGVSKQDLLSVCTAIISQRLYSNGDKKV
                     CVYEIMDQKTLEYVIRYGKYPDQFQTLSSKIKEGIEKGYILDEQAECDMFSLS"
     gene            3948..>4610
                     /locus_tag="ACZPDN_09700"
     CDS             3948..>4610
                     /locus_tag="ACZPDN_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298933.1"
                     /translation="MTNKPSATCLVCREEIEQFQLLQNQGVPFDTLVHLVFKKDDEIL
                     DELKSGNCLMDLFCRNQKKLYFKYLKILGSKMNLKEAIECVTQIEKSSNVFFEQLLKK
                     IAYPFFLLVFAYFMICFFSDFVLVQMKDYISSNSVLILIQVLKVLFGTSILCILLYLG
                     LYYLFFYKYDARLKCPFSLMKKMISLQFVCMYQALEKTYSSTQEVLETLSLMDFSIVG
                     MVS"
BASE COUNT         1591 a          659 c          889 g         1471 t
ORIGIN      
        1 aaaatcattg aaaagggcaa gacaaactat ttctgttgat caagtcattt cgaatcgtat
       61 attgattctt gcggtttcca ttaccgtatg ttttttaatc atctttttac gattgttcta
      121 tcttcagatt gtttcatata atgactatac agcgaaaaga gaagattata cttccatcaa
      181 acaatatact tcagcaccac gtggtcaaat ctatgattgt aagggaagaa ttttagctaa
      241 gactgtggtt agtcacaata ttgtctttac atctccaaac aatatggatt cagatgatta
      301 tgaattgtat gcgaaacgta ttgtgaaagt gtttcgtgta agcgcaaaag attttacaga
      361 ggaagataaa aagcaggcat atattacgta caaatctttt ttatctgtaa atgattctga
      421 atatgcagcc aatcatttat tgacttcaaa agaattgaaa caatacaaaa atggtgcgtg
      481 gggtgacaat gccgaaagta aacgatacca gatcttaatg aatcgtattg gcaagaaaca
      541 aattggtgag atgacacaat ccgaattaaa aatgtgtgtg atttaccaaa gaatgattgc
      601 caaccaaagt acaggacaag aaaatgttgt acttgaagat gtcagcgatg atgatgtggc
      661 ctatcttgtc gaacataaaa cggaatttcc tggatttgac gttgattttg gtggatggaa
      721 acgagaatat ccatatggag aatctttatc ggatgtttta ggaagtgtaa ctacaagtac
      781 acaaggtttg ccgagtgaat tatctagtta ttatctttct aaaggctacc aattaaatgc
      841 gagtgtgggt aaatcaggcc ttgaatatca atataatgat ttacttgcag gaacacctga
      901 aattgccaag attacgtata attcgaaggg attggcgcaa aaagaagtgg ttcaagaagc
      961 aaaaaaagga tatgatatcc atctttctat cgatattgat cttcaaactt cattagatga
     1021 tattttaaag aaaacattaa atgaatatgg gggatcggcc aatcgtgaaa atttcaaatc
     1081 tttatttacg acggtattaa atgcaaaaga tggctcagtc cttgcgatgt caggctatca
     1141 aatggatttg gatacaaaag gtatgacata tttttcaacg ggtaactata tgtctttagt
     1201 taacccagga tcatgcatta aaggggccac tgtatatatg ggtgaaagtg aacatgtgat
     1261 gagtccagga gaaacgattg tcgataaaac aatgaatatt ggtggacaag aatttagttc
     1321 ctacaaagat tatggtacag ttgatgacat tatggcttta gaagttagta gtaacatata
     1381 tatgtttaat gtggcaatac gtttggccaa tgcctcatat caagaagaac aaccgcttta
     1441 cgtgaatgat cttcaaggta cattaaataa aatgcgtggc tactattcga tgtttggttt
     1501 aggcaataaa acaggcattg atgcaccaaa tgaagcaagt ggctatatgg gttatggtgc
     1561 agagccaggt atgttacttt attattctat agggcagttt gatatgtatt caccacttca
     1621 attggccact tatggggcaa ctattgcatc taatgggaat ttatataaac cacgttttat
     1681 gtcttatgct acggaagtga attcacaaga ggtagttgtg acgaatgcta aaaaaattaa
     1741 atctaaattg cctgaaaaga atgcagccta tttagatcga gtcaaacaag gctttagtgc
     1801 ctgtgtaagt tcaggttatt gtggagatcc aatgcagcca tttgcaggta aagtttcagc
     1861 caaaacaggt acagccgaag taggagattg gacaaccgca aaccttgttg gatatgctcc
     1921 aagtgatgat gcaaccatgg catttgcgtg ctcagcacca acatcaagta tcaatgatca
     1981 aagtgtttca ccaaacattt gttcgaatat ggtgatgcca gaatttattc aaaagtattt
     2041 cgaattgtat cctgcaagtt aaaattttga aaatgacttg aaattgactt gtaagttatg
     2101 ctagaatcaa ctagtaatta ctaatgtgag gaggactgaa catgagagat cgtattacat
     2161 ttaagtgctc tgtttgcggt gaagaaaatt atattggtac aagaaataag aaaaaacatc
     2221 ctgaacgtat gactgttaaa aaatattgtc caaaatgcaa tcagcagact gaacataagg
     2281 agaaaaagta agaaaggccg ttttattcgg tcttttttat gtattagacg atgaaagtta
     2341 ttcaacaacc tttaggacct gtacaggcaa attgttattt ggtgatggaa aaccatcatg
     2401 ctttaattat tgatccggcc gacatattgc caaatcttga tgaaattctg aatcagaatg
     2461 attgtacatt agatgcgatt gttttaacac atgcacactt tgatcatatt tcaggcgtag
     2521 ataagattgt aaataaattt gcctgtgatg tatatttaaa tccaaatgaa tttgattttc
     2581 ttttagaccc cgatttaaat tcatccagtg ccttttatat ggatgtgaca tgtaatgcca
     2641 acgcaaaacc acttattgaa ggtaaaaacg aaattgcagg ctttgaagta gaagctatat
     2701 attgtccggg acattcgatt ggttctacag tattaaaaat agaagattgt ttatttactg
     2761 gagacgtgct atttcaaggt tccattggaa gaatggattt agcaactggc agtgtttcaa
     2821 gtatgaaaca atccttaaaa aaattggttc aattcaacaa agattataaa gtatatccag
     2881 gtcatggacc cacaacttct ttatcgcaag agaaaaaatg gaacgaaagt ttaaaatatg
     2941 cagaaattta ttagaatttc tgcttttttt ttcgtattag ataagggggt gataaaaaat
     3001 ggaggattta ttaaaggatc tcattgaata tgcgattgat aaaaaagcga gtgatatcca
     3061 ttttgtacta cataatcaaa ggttacagat tcaacttcgc acacaaaatg aaatgattga
     3121 tgtatatcaa gatatatgga aaccgtcgtt ttttgagtat ttaaaattta tttctcagat
     3181 ggatctaacc aatccttata cgccgcaaag tggacagttt aaagtatatc ttcataatca
     3241 tgatatcttt tgtcgcttca gcatcttgca taatcataat attcaaacag gtgtcattcg
     3301 gttgttgaac actaaacaag ctattcaact ctatgaattg tctttatgga aaaaggatac
     3361 acaatttttt caatctttaa ttccgcttag acaaggactt gtgctatgtg taggacctac
     3421 aaatagcgga aagacgacaa cacttcatgc tttacttcat gaaatcgcaa aaacaaggaa
     3481 acataaggtt gtcagtctag aagatccaat cgaaattgaa gatgattcct atttacagct
     3541 acaaatcaat gaaacaatgg gatttacata cgatgtaggc atcgagcaat tattaaggca
     3601 tgatccggac attatatgta ttggagaaac aagaaatagc tatacagcga agatggtatt
     3661 gcgtgcaagt ttaacagggc atttggtctt aacgagtatt catgccaaaa atggaaagga
     3721 gtgtatcagt cgtcttgaag atcttggcgt ttctaaacaa gatttattaa gtgtatgtac
     3781 cgccatcatt tcacaacgct tatattcaaa tggcgacaag aaagtgtgtg tatatgaaat
     3841 catggatcaa aaaacattgg aatatgtcat acgatatgga aaatatcctg accaatttca
     3901 aacattatct tcaaaaatta aagaaggtat tgaaaaagga tatatccttg acgaacaagc
     3961 cgagtgcgac atgtttagtt tgtcgtgaag aaattgaaca atttcaattg ttacaaaatc
     4021 aaggcgtgcc atttgataca ttggttcatt tggtatttaa aaaggatgat gaaatattgg
     4081 atgaattaaa atccggaaat tgtttaatgg atttattctg taggaatcaa aagaagttgt
     4141 attttaaata tttaaagatt ttaggtagta agatgaatct taaagaagct atcgaatgtg
     4201 ttactcaaat tgaaaaaagt tcgaatgtat tctttgaaca attgttaaaa aagatcgcat
     4261 atccattctt tttacttgta tttgcgtatt ttatgatttg tttcttttca gactttgtgc
     4321 ttgttcaaat gaaagattat atttcaagta attctgtctt aattttgatt caagtattaa
     4381 aggttctatt tggcacaagc attttgtgta ttctattgta tctaggctta tattatttgt
     4441 ttttctataa gtatgatgca agactgaaat gtccattttc tttgatgaag aaaatgatct
     4501 ctttacaatt tgtatgtatg tatcaagctt tagaaaagac ctatagttca acacaagaag
     4561 tattagaaac tttatctttg atggatttta gtattgtggg tatggtatcc
//
LOCUS       DBMDWL010000163         4572 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_7949_length_4572_cov_2.128159, whole genome shotgun sequence.
ACCESSION   DBMDWL010000163 DBMDWL010000000
VERSION     DBMDWL010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7949_length_4572_cov_2.128159"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="ACZPDN_09705"
     CDS             <1..259
                     /locus_tag="ACZPDN_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298934.1"
                     /translation="EIEKDVIKDCLLALPERKNFDTEFKVVPESLNNKRTKKFQLSQG
                     IELTIRSDAMEYPDKIVSTVVDGKRVIQIVCEDDDTYDAFA"
     gene            339..662
                     /locus_tag="ACZPDN_09710"
     CDS             339..662
                     /locus_tag="ACZPDN_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298935.1"
                     /translation="MKKTKSLTILLNVFAVLSLAYTCYAAIQAYNTFISYYSTNIDVL
                     NLVMYMITTCYQSLCFSVLFYALGVFSENFKKPEEPVVVEEKKEPKESEETKTVEENE
                     KKEEK"
     gene            911..1498
                     /locus_tag="ACZPDN_09715"
     CDS             911..1498
                     /locus_tag="ACZPDN_09715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1298936.1"
                     /translation="MERIEKQMEFILEIDKEKQIKRKTLQSNGKNFEDDAQHAWHMAI
                     MTLLLNEYANEEIDVLKTISMLLIHDLVEIDAGDTYAYDSQGLKTQSTREAKAANRIF
                     NLLPEDQAKKMFDLWNEFEERKTAEAKFARVLDNFQPVMLNAATDGRMWVENGVHLEQ
                     ILKRNEKTHEGSEVLWKYMYEHFIMPNVEKKRIKR"
     gene            1569..2315
                     /locus_tag="ACZPDN_09720"
     CDS             1569..2315
                     /locus_tag="ACZPDN_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024743.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR1298937.1"
                     /translation="MKKGFIIVIILVLVCIAGGSFLAIHLITNTNIDIDKVSEEISIA
                     KEPLNHEGIENFTSVNLDLISMDVKVEKSSENAISYRLQDAKKQKLDYSVQNGVLTVK
                     EIVSDKHFVSHDDNEVIIYTTNDSLSVNGNLVAGDVEIQNLTVNDSRVTSVSGDISIE
                     NSNLNNSTFVTTSGDIEFDGQLTGDNTLETTSGDVSVENISNVSVTASTTSGDIEIKE
                     EELTGSVTRVLGSNNATLTLNTTSGDIEIN"
     gene            2417..2563
                     /locus_tag="ACZPDN_09725"
     CDS             2417..2563
                     /locus_tag="ACZPDN_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298938.1"
                     /translation="MTRTSKYLNQDTIAALQSFQIEKIDREITDYLDTNNKMNTYHFE
                     YCLV"
     gene            2596..2820
                     /locus_tag="ACZPDN_09730"
     CDS             2596..2820
                     /locus_tag="ACZPDN_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1298939.1"
                     /translation="MLRCKECKKRFVVDRGQLTFYSHHDQSKWNELILDTLNGVSLKE
                     TAVKINVNERNVFNMRHKLLVSLKTEEHPK"
     gene            3417..4094
                     /locus_tag="ACZPDN_09735"
     CDS             3417..4094
                     /locus_tag="ACZPDN_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6619 domain-containing protein"
                     /protein_id="HIR1298940.1"
                     /translation="MKHKIIVIFVLALMFSLAGCGNTNKLAEYDMTGISFISYADIDT
                     VCEDEELTTAGKKLLSEQEENLLLSYVMDSAIELSAELGNYDHLVFTNPQWIERFGDL
                     EKLKPIEYSNLSKDMQIFLETQMPILTNDGSVLPEGIGLYEYEDGSLFAFPVNVTLGA
                     AEPIEAKKPLIVLIDNPAETLKADSCMLPLTSSGNVLFLDNDNFQQLFENSELKDYGD
                     IQKFDEN"
     gene            complement(4351..4500)
                     /locus_tag="ACZPDN_09740"
     CDS             complement(4351..4500)
                     /locus_tag="ACZPDN_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298941.1"
                     /translation="MLMYKNGIPISNVKDFLGLSDISTTSRYAYADNEDIKKALETVQ
                     EKLSH"
BASE COUNT         1686 a          586 c          875 g         1425 t
ORIGIN      
        1 agaaatcgag aaagatgtta ttaaagattg cttattagca cttcctgaac gtaaaaattt
       61 tgatacggaa tttaaggtag ttcctgaaag cttaaataac aagcgtacaa agaaatttca
      121 attatcacaa ggtattgaat taacaattcg ttcggatgcc atggaatatc cagataagat
      181 tgtatcgact gtagtagatg ggaagcgtgt cattcaaatt gtatgtgaag atgatgatac
      241 atatgatgct tttgcttaaa ataaactgaa agaatttaag tttgtataat tctaaaaata
      301 gtggtttaat tatatcatta tttaaagggg gattttagat gaaaaaaaca aaatcattaa
      361 cgattctatt aaatgtattt gccgttctat cattagctta tacatgttat gcggcaatcc
      421 aagcctataa tacgtttatt tcatattaca gtacaaatat tgatgtattg aatttagtta
      481 tgtatatgat cacaacatgt tatcaatcac tatgctttag tgtgcttttc tatgctttag
      541 gtgtgtttag tgagaacttt aaaaagccag aagaacctgt agtcgttgaa gaaaagaaag
      601 agccgaaaga atcagaagaa acaaaaacag ttgaagaaaa tgagaagaaa gaagagaaat
      661 agttgttcta tttctttttt ttgtattcgc gtttacatat aaatgtaaaa tatttacaat
      721 gtaattgaaa acaattgcaa ataatgatat aattttattg gcttcgaaag gaagttttaa
      781 gaaggtttgc gtcaatagaa atcaattttg ctccttcctc aagttcactg atgatttcgc
      841 atttgacgta atagattagg cctgagtgaa tccgggcctt tttgtgtgtt acaatggttt
      901 tggtgatagt atggaacgaa ttgaaaaaca gatggagttc atcttagaaa tcgacaaaga
      961 gaaacaaatc aaaagaaaaa ctttacagag taatggaaaa aattttgaag atgatgcgca
     1021 acacgcttgg catatggcta ttatgacatt acttttgaat gaatatgcga atgaagaaat
     1081 tgatgtatta aagacaattt caatgttgtt gatccatgac cttgtagaaa ttgatgcggg
     1141 tgatacatat gcctatgata gtcagggttt gaaaacacaa agtacaagag aggcaaaagc
     1201 cgcaaatcgt atttttaatt tattgccaga agatcaggca aaaaaaatgt ttgatttatg
     1261 gaatgaattt gaagaaagaa aaacggctga agctaagttt gccagggtgt tggataattt
     1321 tcagccagtt atgttgaatg cagcaacgga tggaagaatg tgggttgaaa atggtgtgca
     1381 tttagaacag attttaaaaa gaaatgaaaa gacacatgag ggtagtgaag tgttgtggaa
     1441 gtatatgtat gagcatttta ttatgcccaa tgttgaaaag aagagaatca aaaggtgatt
     1501 cttttttgag tatactacta tacaaaacaa agtagcggtg ataaactagt agtgaaagag
     1561 gtgatttgat gaaaaaagga ttcattatag taattattct tgttcttgtt tgtattgcag
     1621 gaggtagctt tcttgcgatt catttaatta cgaatacgaa tattgacatt gacaaagtat
     1681 ctgaagaaat atctattgcg aaagaaccat tgaatcatga ggggatagag aattttacat
     1741 ctgtgaattt agatttgatt agtatggatg ttaaagttga aaaatcgagt gagaatgcga
     1801 tttcttatcg tttgcaggat gcaaagaaac aaaagctaga ttattctgtt cagaatggtg
     1861 ttttaactgt aaaagaaatc gtttcagata aacattttgt aagtcatgat gataatgaag
     1921 tgattattta tacgacaaat gattctttaa gtgtgaatgg taatcttgtt gcgggagatg
     1981 ttgaaattca aaatttaaca gtcaatgatt cgagagtaac cagtgtatct ggggatattt
     2041 caattgagaa ttcaaatcta aataatagta cctttgtcac aacaagtgga gatattgaat
     2101 ttgatggtca attgacaggg gataatacac tagaaaccac ttctggagat gtaagtgtag
     2161 aaaatatcag taatgtttca gtaacagcta gtacaacgag tggagatatt gaaattaaag
     2221 aagaagaatt aactggttct gtaactcgag tattaggctc aaataatgca acattgacat
     2281 tgaatacgac aagtggtgat attgagataa attaaaagga ttaacagata cattggtgac
     2341 atgttgatat attcgacatt aatggtgtct atttaagaat tattgtatag gtgtaaacaa
     2401 aagaaagact acacttatga caagaacttc gaaatattta aaccaagata caattgctgc
     2461 tttacaatct tttcaaatcg aaaagattga tcgtgaaatc actgattatt tagatacaaa
     2521 caataaaatg aatacgtatc attttgaata ctgtctcgtt taattaagag cggttttgcc
     2581 aactctggta aacagatgct tcgttgtaaa gaatgcaaaa aacgttttgt tgtggaccgt
     2641 ggtcagctta ctttttattc tcaccatgat caatctaaat ggaatgaact tatcctggat
     2701 acattgaatg gtgtatctct taaagaaaca gctgttaaaa tcaatgtaaa tgaacgcaat
     2761 gtttttaata tgcgtcataa actattagtt agtctaaaaa ctgaagaaca tcccaaatga
     2821 ggttgaaatg aatgaaaagt atactccata caatcttaaa ggaactaaga ttcatggagt
     2881 ctaacctaaa catcgtggaa cacctgctac aaaaagagga ttatcgaatc aactggtttg
     2941 tatcatcact gcaatacaga gatttgctca tacaattgcc agaacattaa attatggtaa
     3001 gccatcattc atagatctat taagatttgg taaatgtctt gaatcaaaat cttttgtaat
     3061 gctagatggt tcaaacagct acaacgaact tttagaaagt aagaactgca caaagaaagt
     3121 attaatcagt catgagtcat atgataaatt caatcattta aatacagtga acagctttca
     3181 caaatgaatt gaagaagggt tgcaaaaaga taagggagta gcatcgaaat atataaatcg
     3241 ttacaatgct ctttttgtca tgcaaagaga aacgcaagaa atggataata cagaaaagtt
     3301 acagtacgta ctatttagat tgaaaaacga atggttgttc gcataagtga taaaaagtag
     3361 aattatagca atgtcaccaa attgctagtt aagccaaata taaaatggag gtatctatga
     3421 aacataaaat tatagtcata tttgtattgg cgctcatgtt tagtttggct gggtgtggaa
     3481 atacaaataa acttgctgaa tacgatatga caggaattag ttttatatca tatgcagata
     3541 tagatacagt atgcgaagat gaagaattga caactgccgg aaaaaaatta ttgtctgaac
     3601 aagaggagaa tttgctttta tcttatgtaa tggacagtgc tattgagctt tctgctgaat
     3661 tgggaaatta tgaccattta gtgtttacaa atcctcaatg gattgaacgc tttggcgact
     3721 tggaaaaatt aaagcccata gagtatagta atctgtcaaa agatatgcaa atatttttag
     3781 aaactcagat gcctatatta acaaacgatg gaagcgtgtt gccggaagga ataggactgt
     3841 atgaatatga agatggcagt ttgtttgcgt ttcctgtaaa tgtaacactt ggagccgcag
     3901 agccaataga ggcaaaaaaa ccgctgattg ttttaataga caatccagca gaaaccctta
     3961 aagcggatag ctgtatgttg ccattgactt caagcggaaa tgtgttattt ttggataatg
     4021 ataatttcca acagcttttt gaaaatagtg aacttaaaga ttacggagat attcagaagt
     4081 ttgacgagaa ctaacttttc acaattgcat acttacccac aagggacagc cgagaaaatc
     4141 gattgtcctt ttttctatgc caataggcag aaaggagcga ccagacatga cgaaaccgag
     4201 agattatatt cagaaaaaga ttatctctaa ttatttaaat ataaccttat aaaatcgtgt
     4261 atctagaatt aataattgaa gataatgttt tttactctaa gccacaatat tttagtaaat
     4321 cttttccttt ccacttcttt tctttaacac ttaatgagat aatttttctt gaactgtctc
     4381 aagagctttt ttaatatctt cattatctgc gtaggcatat cttgatgtag ttgagatatc
     4441 agaaagacca agaaaatctt taacattaga aatagggata ccatttttat acatcaacat
     4501 ataaccaatg catctgttaa aaaaaaaaaa aaaataggca agcgaaatta atcgtttgcc
     4561 tttaatagtt ca
//
LOCUS       DBMDWL010000164         4555 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8000_length_4555_cov_2.115402, whole genome shotgun sequence.
ACCESSION   DBMDWL010000164 DBMDWL010000000
VERSION     DBMDWL010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8000_length_4555_cov_2.115402"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="scpB"
                     /locus_tag="ACZPDN_09745"
     CDS             <1..375
                     /gene="scpB"
                     /locus_tag="ACZPDN_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1298942.1"
                     /translation="VEYANRFKFVTKEFVYPYGKQLFEEYKQSTLSQAAMETLAIIAY
                     KQPITRVEIEEIRGVNCEMMLKKLVARGLIEAKDRLDAIGKPLLYRVTDEFLDAFQLE
                     DLKELPELPQTQNNNEELFEGE"
     gene            379..1107
                     /locus_tag="ACZPDN_09750"
     CDS             379..1107
                     /locus_tag="ACZPDN_09750"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1298943.1"
                     /translation="MERLQKVIAQAGICSRRKAEELILDGKVKVNGEICDVLGTKVSK
                     QDIIEVNGNMISKEDKVYYIMNKPKGCLCTSSDDKGRKTVLDYMPKNERIFSVGRLDY
                     DTSGVLLLTNDGEFCNHMIHPRYHLEKTYVVNLQGILSEDDIKQLRKGLKTKTEKYQP
                     AKVFVLQKDLTRNRTQFELTISEGKNHQVKNMMLALGHEVRRLHRVKFAFLDVKDLRA
                     GEYRHLKPYEIKQLNRLAMEGENK"
     gene            1107..1826
                     /locus_tag="ACZPDN_09755"
     CDS             1107..1826
                     /locus_tag="ACZPDN_09755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR1298944.1"
                     /translation="MKQVYLDAVLSIHDVIELDTKQAHHIFDVLRTTPKEKIRIITKN
                     SGVFLGHVENKPTLIIDSIVDVFEENQSITLCCALIKQDKFEWMLQKACELGVTKIVP
                     FVSKNTVVKLDEKKAEKKQSRWNEILLAATKQCNRNTLVELEPVIPLKKLSNYKSECN
                     LVAYEKESDPSKHMAHYLKENPKSITVCIGPEGGFEESEIQVLNEFGYENCSLGKNIL
                     RAETAACYVLSAIEYQNHVEG"
     gene            1834..3111
                     /gene="mtaB"
                     /locus_tag="ACZPDN_09760"
     CDS             1834..3111
                     /gene="mtaB"
                     /locus_tag="ACZPDN_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR1298945.1"
                     /translation="MKFSIITLGCKVNAYESQYYAGQLEKLGYEQVSPEKACDICIIN
                     TCTVTNTAASKSRQKIHYAKRMNPNALCVVVGCFVQCASEEERKALDADLIVGAKQKN
                     ELVNLIQQALKDHEKIDVVHEVTQFKDFEAMPVHCFESMHRAFLKVQDGCNQFCSYCA
                     IPFARGRERSLNHEQVIQIAKDLCDKGHTEIVLTGIHTGRYFDGEYDLAKLMKALLEN
                     TPDYVYYRISSIEITEVSDELIGLMQENSRMCHHLHIPVQSACNETLKRMNRPYTIEQ
                     FKEKIEYIRSCIPDISISTDVIAGFVQESDEEFETTYKNLAELELSFFHVFPYSKRNG
                     TKASTLKGEINGKIVKARVARLLELSNECRKRDMQRFNPVQVLIERQQDGYYTGYTNQ
                     YHPCKILSDIPLSGRITIEWQRIEDGTYYGKKD"
     gene            3114..>3672
                     /locus_tag="ACZPDN_09765"
     CDS             3114..>3672
                     /locus_tag="ACZPDN_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR1298946.1"
                     /translation="MRLSKLFKNAPTVNITGLSFDSRTVRPGNIYFCLPGLTNDGHDF
                     IDSAIKNGAVCIVHSKELLNMAPGAVYIRVEDVNDAMNKVARIFYAKPSDKLKMYGVT
                     GTNGKSTITNIIRDMINDKTPCGYIGTIAIKYGDIELQPNLTTPDALFLQSKLADMVR
                     VGMKACALEVSSHGLAQHRVDGISFD"
     assembly_gap    3673..3682
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3683..4546
                     /locus_tag="ACZPDN_09770"
     CDS             <3683..4546
                     /locus_tag="ACZPDN_09770"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR1298947.1"
                     /translation="TYDHLDFHGTMENYFEAKSLLFKNLVKENGVSVLNRDDEKYEAL
                     KDCSKARVVSYGINKEADYRAINIKMSSQGTQFDLVYSGHMYPIKTNLVGNFNVYNLL
                     AAMCALNETGYDLDKIIEKCSHIAQVEGRMERIDCGQPYNVIVDFAHTPDGMEKMMQF
                     GRNITMPGHRLIAVFGSAGKRDVHKRKVFGELADKYCDYIIVTEDDPRDEDPKEIADQ
                     IKSGIQNTLHVFVQDRYEAIHQAISSAQEGDTVLLLGKGDESFIYRENGRAPWVGDNV
                     AAKECIQANLG"
BASE COUNT         1623 a          620 c          926 g         1376 t
ORIGIN      
        1 gtagaatatg cgaatcgatt taaattcgtc acaaaagaat ttgtatatcc ttatggaaaa
       61 caattatttg aagaatataa acaatccaca ctatcacaag cggctatgga aactttggct
      121 atcattgcct ataaacaacc aattactcgt gtggaaatcg aagagattcg tggtgtcaat
      181 tgtgaaatga tgttaaaaaa attagtggca cgtggcttga ttgaagctaa agatcgtttg
      241 gatgcgattg gcaaaccttt attatatcgt gtaacggatg aatttttaga tgcttttcag
      301 ttagaggatt taaaagaatt accggaatta ccacaaacac aaaacaataa tgaagaatta
      361 tttgaaggag aatagaaaat ggaaagacta caaaaagtaa ttgcccaagc gggtatttgt
      421 tcaagaagaa aagcagaaga attgatttta gatggaaaag tcaaagtgaa tggtgaaatt
      481 tgtgatgtat taggtacaaa agtatcaaaa caagatatca tcgaagtcaa tggcaatatg
      541 atttcgaaag aagataaagt ctattatatc atgaataaac ctaaaggttg tctatgtaca
      601 agtagtgatg ataagggaag aaaaacggta ctagactaca tgcctaaaaa tgaaagaatc
      661 ttctctgtag gacgtttaga ttatgacaca agtggcgttt tattattgac aaatgatggt
      721 gaattttgta accatatgat tcatcctcgt tatcatttag aaaaaacgta tgttgttaat
      781 cttcaaggta tcttgagtga agatgatatc aaacaattaa gaaaaggttt aaagacaaaa
      841 acagaaaaat atcagccggc taaagtattt gtgctccaaa aagatttaac gcgtaatcgt
      901 acgcagtttg agcttacaat ttctgaaggt aaaaatcatc aggtaaagaa tatgatgttg
      961 gcattaggcc atgaagttcg tcgcttgcac agagtaaaat ttgcgttttt agatgttaaa
     1021 gatttaagag cgggtgaata tcgtcattta aaaccttatg aaatcaaaca attaaatcgt
     1081 ttagctatgg aaggcgaaaa caagtaatga aacaagtata tttagatgcg gttttatcga
     1141 ttcatgatgt gattgaatta gatacaaaac aagcacatca tattttcgat gtacttcgta
     1201 caacaccaaa agaaaagatt cgtatcatca caaaaaattc gggtgttttc ttaggacacg
     1261 ttgaaaataa acccacacta atcattgatt ccattgtaga tgtgtttgaa gaaaaccaat
     1321 caatcacatt atgttgtgct ttgatcaaac aggataaatt tgaatggatg cttcaaaagg
     1381 catgtgaact gggtgtaact aaaatcgttc cttttgtatc gaagaatacg gttgttaaat
     1441 tggatgaaaa aaaggcagaa aagaaacaat ctcgatggaa tgaaatctta cttgcggcaa
     1501 caaaacaatg taaccgcaat acattggtgg aattagagcc tgtaattcct ttaaaaaaac
     1561 tgtcgaatta taaaagtgaa tgtaatttgg tggcttatga gaaggagagc gatccttcta
     1621 aacatatggc acactattta aaagagaatc ccaaatccat tactgtatgt attggaccgg
     1681 agggtggatt tgaggaaagt gaaattcaag tattaaatga atttggttat gaaaattgtt
     1741 ctttaggtaa aaatatttta cgagcggaaa cagcagcttg ttatgtatta agtgcaattg
     1801 aataccaaaa tcatgtggaa ggataaaaaa tcaatgaaat tttcaattat tacactcggc
     1861 tgcaaagtaa atgcgtatga atcgcaatac tatgcaggtc aattagaaaa actaggatat
     1921 gaacaagtat cgcctgaaaa agcttgcgat atttgtatca tcaatacatg tactgtcaca
     1981 aatacggcag ctagtaagtc acgtcaaaag attcattacg caaaacgtat gaaccctaat
     2041 gccttatgtg ttgtggtagg atgttttgta caatgtgcaa gtgaagaaga aagaaaagcg
     2101 ttagatgcag atcttatcgt tggggctaaa caaaagaatg aattggtgaa tttgatccag
     2161 caagcattaa aggatcatga aaaaattgat gttgttcatg aagttacaca gtttaaagat
     2221 tttgaggcga tgcctgtaca ttgttttgaa tcgatgcatc gagcctttct aaaagtacaa
     2281 gatggctgta atcaattctg ttcatattgt gcaattcctt ttgcgcgtgg tcgagagcga
     2341 agtttaaatc atgagcaggt cattcaaatt gccaaagatc tatgtgataa aggacataca
     2401 gaaattgttc taaccggtat tcatacaggt cgatattttg atggagaata cgatttagca
     2461 aaattaatga aagctttatt agaaaataca ccagactatg tatattatcg tatttcaagt
     2521 atcgaaatta cagaagtgag tgatgagttg attggattga tgcaagaaaa ttctcgtatg
     2581 tgtcatcatt tgcatattcc tgtacaatct gcatgcaatg aaacattaaa acgtatgaat
     2641 cgtccgtata caattgaaca attcaaagaa aaaattgaat atattcgttc ttgcattcca
     2701 gatatttcaa tttctaccga tgtgattgct ggatttgttc aagaaagtga tgaggaattt
     2761 gaaacaacgt ataaaaatct agccgaacta gaattaagct tcttccatgt tttcccttat
     2821 agtaagcgta atggaacaaa ggcaagtact ttaaagggcg aaatcaatgg taagattgtc
     2881 aaagctagag tggctcgttt actcgaactt tcaaatgaat gtagaaaacg tgatatgcaa
     2941 cgttttaacc ccgtgcaagt gttgattgaa agacaacagg atggctatta tacgggttat
     3001 acaaatcaat atcatccttg taagatattg agtgatatac cattaagtgg tagaatcact
     3061 atcgaatggc aacgtattga agatggtacg tactatggaa agaaggatta agtatgcgat
     3121 taagcaaact atttaaaaat gcacctacag tgaacattac aggcttaagc tttgattcaa
     3181 gaacagtaag acctggaaat atctatttct gtttacctgg ccttacaaat gatggacatg
     3241 actttattga ttctgccata aaaaatggag ccgtatgtat tgttcattct aaagaattat
     3301 tgaatatggc acctggtgct gtttatattc gtgtggaaga tgtcaatgat gcgatgaata
     3361 aggtggctcg tattttctat gccaagccta gtgataaact aaaaatgtat ggtgttactg
     3421 ggacaaatgg aaaatcaaca atcacaaata tcattcgtga catgatcaat gataaaacgc
     3481 cttgtggtta tattggtacc attgcgatta aatatggtga tattgaattg cagccaaatt
     3541 taactacacc agatgcactt tttttacaat ctaaattagc cgatatggtt cgtgtgggaa
     3601 tgaaggcatg tgcattagaa gtatcaagtc atggattagc tcaacatcgt gtggatggta
     3661 tttcatttga ttnnnnnnnn nnacatatga tcatttagat tttcatggaa caatggaaaa
     3721 ttattttgag gcgaagagct tgttgtttaa aaatcttgtc aaagaaaatg gtgtttctgt
     3781 tttaaatcgt gatgatgaaa aatacgaggc cttaaaagat tgctccaaag cacgtgttgt
     3841 atcttatggt attaataaag aagcggatta tcgtgctatt aatattaaga tgagttcaca
     3901 aggaacacaa tttgatttag tatactcagg tcatatgtat ccaatcaaga caaatttggt
     3961 gggcaacttc aatgtatata acttacttgc agctatgtgt gctttaaatg aaacgggtta
     4021 tgatttagat aaaattattg aaaaatgcag tcatattgca caagtagaag gtcgtatgga
     4081 acgtattgat tgtggtcaac catataatgt gatcgttgat tttgcgcata ctccagatgg
     4141 tatggaaaag atgatgcagt ttggaagaaa tatcacaatg ccaggacatc gtttaattgc
     4201 ggtatttggt tctgcaggta aacgtgatgt acataagcgt aaagtatttg gcgaattagc
     4261 tgataaatat tgtgattata ttattgttac ggaagatgat cctcgtgatg aagatccaaa
     4321 agaaattgca gatcaaatta agtctggaat tcaaaataca ttacatgtat ttgtgcaaga
     4381 tcgttatgaa gcaattcatc aagctattag ttcagctcaa gaaggtgata ccgttttact
     4441 tcttggtaaa ggagatgaaa gctttattta tcgggagaac ggaagagctc cttgggtagg
     4501 cgataatgta gcggctaaag agtgtattca ggcaaattta ggttaaaatt ttata
//
LOCUS       DBMDWL010000165         4515 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8092_length_4515_cov_2.863616, whole genome shotgun sequence.
ACCESSION   DBMDWL010000165 DBMDWL010000000
VERSION     DBMDWL010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8092_length_4515_cov_2.863616"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="ACZPDN_09775"
     CDS             complement(<1..303)
                     /locus_tag="ACZPDN_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298948.1"
                     /translation="MKILRLIDQCIDSFEKRIFLFIFFLCLYWLWQNVWQLILFGKLF
                     FVTDYESLFHIQSTLSMYQSSVFIRILYYVISNPTLNVLKLISCIKLIDILAIAGVI"
     gene            complement(281..802)
                     /locus_tag="ACZPDN_09780"
     CDS             complement(281..802)
                     /locus_tag="ACZPDN_09780"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR1298949.1"
                     /translation="MLHVVLYEPEIPQNTGNIIRTCMATGSRLHLIEPLGFSLDEKHL
                     RRSGMDYIKDADIHIHKNWDAFVAENPCEHYYFMTRYGKKAPSEFDFTKEEGDVYLVL
                     GKESTGVDKKILKEHLDTCMRLPMVANARSLNLSNCAAIIVYEVLRQLDYPGLSTHEE
                     LKGEDWLENLKVD"
     gene            complement(805..1380)
                     /gene="rdgB"
                     /locus_tag="ACZPDN_09785"
     CDS             complement(805..1380)
                     /gene="rdgB"
                     /locus_tag="ACZPDN_09785"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1298950.1"
                     /translation="MKKTIWIATSNAHKVEEFQTMLKDCDVKCLKDLDHTIHIIEDGT
                     TFEENALIKARTLFNELHEPVISDDSGLEVDAMDKKPGVYSARFLGEDTSYDIKNQYI
                     IDQVQGKVRTARYVCVIAYIDEDGKEHVYRGECEGLIHDKLEGENGFGYDPIFYYPAF
                     KTTLANVSEEKKNSVSHRGIALEKFLKDWSK"
     gene            complement(1492..1770)
                     /locus_tag="ACZPDN_09790"
     CDS             complement(1492..1770)
                     /locus_tag="ACZPDN_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298951.1"
                     /translation="MSEKIDIATELVFSSVANIVSDWQATLNAVELVLYTIHIKENSE
                     IKMNLFLFLLKFEDNILLPILATSNKESTNVIVVLDKFASCDIAIGHI"
     gene            1877..3088
                     /gene="tgt"
                     /locus_tag="ACZPDN_09795"
     CDS             1877..3088
                     /gene="tgt"
                     /locus_tag="ACZPDN_09795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR1298952.1"
                     /translation="MEYKNDNKFYFEIEKRIPGHLGRAGIIHTPHGDIKTPAFMCVGT
                     HGEVRFVSMEELKSINAQAMLSNGYHLRNISPEIAANGGLSAWSKWDGPTLTDSGGFQ
                     VMSLGSGCGKVVSMKREDNMKNTEEEKRLAHVTEDGVHFHDPFTGQEDFIGPEESMQI
                     QCRIGADIHMAYDELTSLADTYEYNVESLARTERWAKRSLDEHKKHCDDLGYHQSLYG
                     VIQGGRWEDLRRSTCKKYAQMDFDGYGLGGAFLKETLGDILTWCNEELPENKPRHLLG
                     LSHPDDILIGTENGADTFDCVAPTREARHGRLYTMHGPINLSKYRDSDEIIDEGCDCP
                     TCQSKITLGELRRLWKSQDRTESAKYFELASMHNLRFIIRLTEQARQAILDGTFKEFK
                     ESFLNSYYGNK"
     gene            complement(3133..4233)
                     /locus_tag="ACZPDN_09800"
     CDS             complement(3133..4233)
                     /locus_tag="ACZPDN_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR1298953.1"
                     /translation="MLNIKYDIVGSFLRPQEIKEARAKFNNNEITYEQLRDIEDEQIA
                     KLVAKEVQHGLKFVTDGEFRRRWWHLDWLKEFDGFTTKHLDKFINGRNNHIELGMIEG
                     KIFYDPNKAHKEMYAWDFLKKEASKYGVEAKKCISGPNMILIDHFLQLGIKDTPYYGT
                     DIEAVIEDIGLAYQAAIRDFYNHGCRYIQIDDTSWTYMIDDTFVSKVEGLGYTKAQVL
                     DWFVRVSTKALENRPEDMQIATHFCKGNFKGNPLFHGFYDTIAKSVASIPYDGYFVEY
                     DDARSGTFDPWAVLKGTDRTFVAGLISTKRKELEEHDAIKARYEQAKAVVGENIALSP
                     QCGFASVEEGNCIDEETQWKKIDLLVSCQDFI"
BASE COUNT         1525 a          843 c          689 g         1458 t
ORIGIN      
        1 tatgactcct gcaatagcca aaatgtctat tagttttata caactaatga gttttaatac
       61 attcaaagtt ggatttgaaa tcacataata taggatacga atgaaaacac tcgattgata
      121 catacttagt gttgactgaa tatgaaataa actctcataa tccgtcacaa agaataattt
      181 accaaacaga atgagctgcc atacattctg ccacaaccaa tataaacaaa gaaagaaaat
      241 aaaaagaaag attcgcttct caaacgaatc tatgcattga tcaatcaacc ttaagatttt
      301 caagccaatc ctctccctta agctcttcat gcgtactaag tcctggataa tcaagttgac
      361 gcaatacttc atagacaata atggcagcac aattggacaa attcaagctt cttgcgtttg
      421 cgaccatagg aagacgcata caagtatcta agtgttcttt gaggattttt ttgtctacac
      481 ccgtactttc ttttccaagc actaaataaa catcaccctc ttcttttgtg aagtcaaatt
      541 cacttggtgc cttttttcca tagcgagtca taaaataata atgttcacat ggattttcag
      601 ccacaaacgc atcccaattc ttgtgaatat gaatatcggc atctttaata taatccattc
      661 ccgaacgacg caaatgtttt tcatccaacg aaaaacccaa tggttcaatc aaatgcaaac
      721 gagaacctgt agccatacaa gttcgaataa tatttcctgt attttgtgga atttccggtt
      781 catacaatac aacatgcaac atacttattt actccaatct tttaaaaact tctcaagcgc
      841 aataccacga tgtgaaacac tatttttctt ttcttcactc acattggcaa gcgttgtctt
      901 aaatgcagga taatagaaaa taggatcata accaaatcca ttttcacctt ccaatttatc
      961 gtgaatcaaa ccttcacatt caccacgata cacatgttct tttccatctt catcaatata
     1021 ggcaataaca cacacataac gagctgtacg taccttacct tgtacttgat ctataatata
     1081 ttgattctta atatcatagc ttgtatcttc acctaaaaaa cgagcggaat aaacacccgg
     1141 ctttttgtcc atcgcatcca cttccaaacc tgaatcatca ctaatgacag gttcatgtag
     1201 ttcattaaat aatgtacgag ctttaatcaa tgcattttct tcaaaagttg ttccatcttc
     1261 aataatatgg attgtatgat ctaaatcttt taaacatttc acatcacaat ccttcaacat
     1321 cgtctgaaac tcttccactt tatgagcatt gctcgttgct atccaaattg ttttcttcat
     1381 aaattatcac cgatatatat tatagaaaga aaaaatgggg ttttcaataa aacttatggt
     1441 aaaataaaaa tcgtttttga aagataaata tttattgttt gttagtgtgt actatatatg
     1501 acctatagct atatcgcaag aggcaaattt atccaataca acaattacat ttgtactttc
     1561 tttattggat gttgccagaa tcggcaaaag tatattgtcc tcaaatttca ataagaacag
     1621 aaagagattc atcttaattt cactattctc tttaatgtgt attgtataaa gaactagctc
     1681 tactgcattt aatgtggctt gccaatcaga aacaatgttg gcaactgatg aaaatacaag
     1741 ttctgttgcg atgtcgattt tctcagacat ctgatctagg tattagatat gttgcaggta
     1801 taatcagtat acttggcata atcttttata taagccgacg ctaatatatg ataaaataaa
     1861 tgagttggag gcatttatgg aatataaaaa tgacaataaa ttttattttg aaattgaaaa
     1921 aagaattcct ggccatttag gaagagccgg aattatacat acacctcatg gtgacattaa
     1981 aacgccagca tttatgtgcg taggaacaca tggtgaagtt cgttttgttt ctatggaaga
     2041 attaaagagc attaatgctc aagccatgtt aagtaatgga tatcatttac gaaacatttc
     2101 cccagaaatt gctgctaatg gaggcttatc tgcatggtca aaatgggatg gtccaacctt
     2161 aacggattct ggtggatttc aagtcatgtc tttaggttca ggttgtggaa aagttgtcag
     2221 catgaaacgt gaagacaata tgaaaaatac ggaagaagaa aaacgtttag cccacgtaac
     2281 agaagatgga gtacactttc atgatccatt tacaggtcaa gaagatttta ttggtcctga
     2341 agaaagtatg cagattcaat gtcgtattgg agccgatatc catatggctt atgatgaatt
     2401 aacttcttta gcggatactt atgaatataa tgttgaatcc ttggcaagaa cggagcgttg
     2461 ggcaaaaaga agtcttgatg aacataaaaa acattgtgat gatttaggat atcaccaatc
     2521 tctatatggc gtaattcaag gtggtcgttg ggaagatctt agaagatcta catgtaaaaa
     2581 gtatgcacaa atggatttcg atggctatgg attaggcggt gccttcttaa aagaaacatt
     2641 aggcgatatt ttaacttggt gtaacgaaga actacctgaa aataagccaa gacatttatt
     2701 aggtttatct catccagatg atatcttgat tggtactgaa aatggtgcag atacatttga
     2761 ttgtgttgcc cccacaagag aggcaagaca tggaagattg tatacaatgc atggtcctat
     2821 caacctatct aaatatagag atagcgatga aatcattgat gaaggatgcg attgtccaac
     2881 atgtcaaagt aaaatcacat taggcgaatt aagaagactt tggaaatcac aagaccgcac
     2941 agaatcagcc aaatattttg aactagcttc tatgcataat cttcgtttca tcattcgtct
     3001 tacagaacag gctagacaag ccattttaga tggtacattt aaagaattca aagaatcttt
     3061 cttaaattca tactacggga acaaataatt gttccttttt ttgtgaaaaa aagagaaatc
     3121 gaacgatttc ccttaaatga agtcttggca agataccaat aaatcaattt tcttccattg
     3181 tgtttcttca tcaatacagt ttccttcttc aacagaggca aatccacatt gtggagaaag
     3241 tgcaatattc tcaccaacta cagctttagc ttgttcataa cgagctttaa tcgcatcatg
     3301 ctcttctaat tctttacgct tagtcgaaat caagccagct acaaatgtgc ggtcagtgcc
     3361 ttttaaaaca gcccaaggat caaaggttcc actacgagca tcatcatatt ctacaaaata
     3421 gccatcgtat ggaatacttg cgaccgattt ggcaatcgta tcatagaatc catggaataa
     3481 tggatttcct ttgaagtttc ctttacagaa atgtgttgcg atctgcatat cttctggacg
     3541 attttctaaa gcttttgtac ttacacgcac aaaccaatct agtacttgtg cttttgtata
     3601 acccaaacct tctacctttg atacaaacgt atcatcgatc atataggtcc atgaagtatc
     3661 atcaatttga atatagcgac atccatgatt atagaaatca cgaatggctg cttgatatgc
     3721 caatccaata tcttcaatca ccgcttcaat atccgtacca taatatggtg tatctttaat
     3781 gcctagttgt aagaaatgat caatcaaaat catgtttggt ccagaaatac atttcttggc
     3841 ttctacacca tatttacttg cttccttctt taagaaatcc catgcgtaca tttctttatg
     3901 tgccttattt ggatcataaa aaatctttcc ttcaatcata cccaattcaa tatgattatt
     3961 tcttccatta ataaacttgt ctaagtgttt tgtcgtaaaa ccatcaaatt cttttagcca
     4021 atccaaatgc caccagcgac gacgaaactc tccatccgtc acaaacttta atccgtgttg
     4081 tacttctttg gcaaccaatt ttgcgatttg ttcatcctca atatcacgta attgttcata
     4141 tgtaatctca ttattattaa atttagcacg agcctcttta atttcttgtg gtcttaaaaa
     4201 agatcctaca atatcatatt taatattcaa cattagtcaa tttcctccaa caacatcatt
     4261 ttatcatttc ttattgatta tttataatat ttaaaaatat gcacttgtta tagtttttat
     4321 ctataatcaa aaagaaaaga atgtgataaa atacattcta tgaacacaaa agaatatata
     4381 cttaatcaat ttaaattata cccacaactt gaattacaag accttatgaa gtttttgtat
     4441 caaagtagtt ttggatgtga acaccttgtt tctgatttct ctaccgtcaa atcaaatatt
     4501 gaacaagaac tacaa
//
LOCUS       DBMDWL010000166         4473 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8205_length_4473_cov_2.680695, whole genome shotgun sequence.
ACCESSION   DBMDWL010000166 DBMDWL010000000
VERSION     DBMDWL010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8205_length_4473_cov_2.680695"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="ACZPDN_09805"
     CDS             complement(<1..596)
                     /locus_tag="ACZPDN_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298954.1"
                     /translation="MEKVVYYCPNCGKTVSRLKGNKDNCSNCNGKMVQTPIDVEKWKE
                     LSDDEKAKIKSEISSYGIPEYSGEPLSVLKHRYDLIQIQKISVTTTDIKRDYVIIGPV
                     FYQINDAGSGKMIFQKQKEYRSVINALKDQNQLVNQNASITESIGALSGMIELFSTGD
                     ISASTKDLLGNGHNQFDEAFFISVEEIKKRAYYMGADA"
     gene            complement(656..1069)
                     /locus_tag="ACZPDN_09810"
     CDS             complement(656..1069)
                     /locus_tag="ACZPDN_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1298955.1"
                     /translation="MKIVEEHIGSRIYKIRKHFNLSMEKFGKQIGISKGSINNIEKGT
                     TNPSSQTINSICREFNVDYVWLTEGIGDNMFISIPNSKIDQLFEDYGLKPEDKWLVRG
                     YLEAPPDIKKQVVNYLQSIVVRELAKREKEKSNKK"
     gene            1488..1904
                     /locus_tag="ACZPDN_09815"
     CDS             1488..1904
                     /locus_tag="ACZPDN_09815"
                     /inference="COORDINATES: protein motif:HMM:NF020049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1298956.1"
                     /translation="MKDHIETLCLERFLQFPPHYLLNNGTGWTNDTGIDSNSYIPGGS
                     AQSTPTVLNSVPSDFIRESATFYPNTTLKIRKAPTESDVDTGLIYAPGMSVCYDGYVK
                     REGFVWISASTGERRWMKAGVLNSKGYNTNPYGRFV"
     gene            2112..2187
                     /locus_tag="ACZPDN_09820"
     tRNA            2112..2187
                     /locus_tag="ACZPDN_09820"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Asp,seq:gtc)
     gene            2266..3120
                     /locus_tag="ACZPDN_09825"
     CDS             2266..3120
                     /locus_tag="ACZPDN_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HIR1298957.1"
                     /translation="MIINVSGRTDVVAYYMDWFVNRWKEGYFDVRNPFNPKLVSRIFV
                     SDVDMIVFCTKNPLPLLDTIHLFSVPIQLQVTITGYFKDMEPNVLDKKQVIECIKKLS
                     SYLGKENVCVRYDPILLNSKYNVDYHVRAFNKLCTMLKGYVSKMIVSFVDDYKNVRNN
                     HLDYHEPSNEEYLKLKEEFEKNDIKIISCMENKYQIGDEKDCCVSIKYAFERTGKLFK
                     EWKARDCRCVNMVDVGAYNSCLHGCKYCYANFDSKQIVSNYKMHDVNSSLLIGQLNLD
                     DQIKIRRK"
     gene            complement(3132..3701)
                     /locus_tag="ACZPDN_09830"
     CDS             complement(3132..3701)
                     /locus_tag="ACZPDN_09830"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025105.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR1298958.1"
                     /translation="MKKFLLILLSLTLCACQTQTKSTTSTSKKACDVTSEEKTCNTKD
                     DTNTNFNEISFDEAIQYFTQEKSGVLYFGFSSCPWCKEVKPILKKVAKENGIDIQYVK
                     VRDEEKNRLYTDDQKAIIEPYIQDYMSNNDEGVLTLYVPLVLVVKDGKVIDGHEGTLE
                     SHDATERKMTNDEKEQLTKIYTKLMSEAN"
     gene            complement(3775..3851)
                     /locus_tag="ACZPDN_09835"
     tRNA            complement(3775..3851)
                     /locus_tag="ACZPDN_09835"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3815..3817),aa:His,seq:gtg)
     gene            3956..>4473
                     /locus_tag="ACZPDN_09840"
     CDS             3956..>4473
                     /locus_tag="ACZPDN_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR1298959.1"
                     /translation="MKVKEMEVEQRPREKALRYGLESLSDLELVALILQSGNKNRSVF
                     EIASDVLKESEGLSKLMTMHVNTLMQIQGIREVKALQLLASVELSKRVIKSKVYHAPI
                     LKPEDVIEWFKFEYGTMSQECFIALYLDTKSKLISHRVLFKGTLNESVVHPREVFKEA
                     FLQNANSVLIAH"
BASE COUNT         1455 a          685 c          772 g         1561 t
ORIGIN      
        1 atagcatctg cacccatata atatgcacgc ttttttattt cttctaccga aataaaaaag
       61 gcttcatcaa attggttatg gccatttcct aacaaatctt ttgtggatgc tgaaatatca
      121 cctgtactga ataattctat catccccgat aatgctccta tcgattctgt tatggatgcg
      181 ttttgattaa ctagttgatt ttgatctttt aatgcattga ttacactacg atattctttt
      241 tgcttttgaa aaatcatttt tcctgatcct gcatcattta tttgataaaa tacaggccca
      301 ataataacat aatcacgttt tatgtctgtt gtagtgacgg aaatcttctg aatttgaatt
      361 aagtcatatc tatgctttaa aacgcttaat ggttctccac tatattcagg gattccataa
      421 gatgatattt ctgacttgat ttttgctttt tcatcatcag acaactcttt ccatttttct
      481 acatcaatcg gtgtttgaac cattttccca ttgcagttag aacaattgtc tttatttcct
      541 tttaatctag aaactgtttt tccacaatta gggcaataat aaactacttt ttccattact
      601 attcctccat aaataagcta tgtacatatt taatttataa aacaaagagt aacagtcatt
      661 tcttgttact cttttctttt tctcttttag ctagttccct gacaactatt gattgcaaat
      721 aatttacaac ttgctttttt atatcaggtg gtgcttcgag atatcctcga acaagccatt
      781 tatcttctgg ttttaatccg taatcttcga atagttgatc tatctttgaa ttgggtatgg
      841 aaatgaacat attgtctcca ataccttcag ttaaccatac atagtcaacg ttgaattctc
      901 gacatataga attgatggtt tggcttgatg ggttagtagt tcctttttca atgttattga
      961 ttgaaccttt tgagatacct atttgtttac caaatttttc catactcaaa ttaaagtgtt
     1021 tacgtatttt atatattctc gaccctatat gttcttccac gattttcacc tttcaactgc
     1081 ttacactgtg attatacatt aaaaagtatg tctaatatac aaaaaaacaa attatttgta
     1141 taatttaaag ttgatatagt atgcaacaca tccttttaat gtatgtgtta catacaaaaa
     1201 gtattttaaa aggaaggatg taataactga tgagtaaaat gtcactaaag atgctttgga
     1261 tatgtttgca aagatgggat atgaaatcaa tgagtatgac cgaggcttta tcgcatgtat
     1321 tttgactcag agcaacagaa aatgttcaga aggaaaggag aacgaagaca aatgaaagca
     1381 tatgtgactg ttagagatgt gatgcttgtt tttacctgta aaggatacac aagctagaaa
     1441 gattttacat aatctacgca gacaaaaaaa taaaaaaggt gaaatatgtg aaggatcata
     1501 tcgagacact atgcttggaa agattcttgc agttcccacc ccattatttg ttgaataatg
     1561 gtactggatg gacaaatgat acaggtattg attcaaattc ttatattcct ggaggaagtg
     1621 ctcaatcaac accgactgta ttaaacagtg ttccttctga ttttattaga gaaagtgcta
     1681 cattctatcc aaatacaact ttgaaaatta gaaaagcgcc tacagaaagt gatgtggata
     1741 ccggtttgat ctatgcgcca ggtatgtctg tatgctatga tggctacgtt aaacgcgaag
     1801 gattcgtttg gattagtgct tcaacaggtg aacgtcgttg gatgaaagct ggtgtgttaa
     1861 attcgaaagg atacaatacc aatccatacg gaagatttgt ttaaggatga aatttataaa
     1921 aaataaaatt taaaaaaaga ttgacataat atagtttata ctgtattatc tttcttgcgt
     1981 gaagcagtga ggtacatttt ggggtacaga acaacaaagt gttatcaaaa cacgcagata
     2041 atgatgtaaa taacatcaaa tattaataga tatgatgtat ttataatctg ctccatttaa
     2101 aattaaatat tggtccagtg gtgtagtggt taacatgcct ccctgtcacg gaggagatcg
     2161 tgggttcgag tcccatctgg accgccattt agaaattgaa gtcatctttg gatgactttt
     2221 tttctttata ttgactattt ttttgtttct tagtttaata aacctatgat tattaatgta
     2281 agtggaagaa cagatgttgt cgcctattat atggattggt ttgtgaaccg gtggaaagaa
     2341 ggatattttg atgtaagaaa cccttttaat ccaaaacttg tcagtcgtat ctttgtatcg
     2401 gatgtagata tgattgtatt ttgcacaaaa aatccattgc cattgttaga tacaatacat
     2461 ttattttctg tacccattca gctacaagtt accatcactg gatactttaa agatatggag
     2521 cctaatgttt tggataagaa acaagtcatt gaatgcatta aaaagctatc atcttattta
     2581 ggcaaagaaa acgtatgtgt tcgatatgat cctatccttt tgaattccaa atataatgtt
     2641 gattatcacg ttcgtgcttt taacaaatta tgcacaatgt taaaaggata cgtatctaaa
     2701 atgattgttt cttttgtgga tgattataaa aatgttcgaa ataatcattt ggattatcat
     2761 gaaccctcta acgaggaata tcttaaatta aaagaagagt tcgaaaaaaa cgatataaag
     2821 atcattagtt gcatggaaaa taaatatcaa attggggatg aaaaggattg ttgcgtcagt
     2881 attaaatatg cttttgaaag aacaggcaag ctttttaaag agtggaaggc aagagattgt
     2941 cgttgtgtga atatggtaga tgttggtgct tacaattctt gtttacacgg atgtaaatat
     3001 tgttatgcca attttgattc gaaacaaatt gtgtcaaact ataaaatgca tgatgtgaat
     3061 tctagtttat tgattggaca attgaatttg gatgatcaaa tcaaaatacg aagaaaataa
     3121 aatgggtgaa gtcaattggc ttcactcatt aattttgtat agatttttgt tagttgctct
     3181 ttttcatcat ttgtcatttt tctttcggtc gcatcatgac tttctaatgt tccttcatgt
     3241 ccatcaatga cttttccatc ctttaccaca agtactaaag gaacgtataa ggttagaaca
     3301 ccttcatcat tattggacat ataatcttgg atatatggtt caataatcgc tttttgatca
     3361 tccgtataaa gacgattttt ttcttcatct cttaccttta catattggat gtcgattccg
     3421 ttttctttgg ccactttctt taaaatgggt tttacttctt tgcaccaagg acaacttgaa
     3481 aatccgaaat ataaaacacc tgatttttct tgtgtaaaat attgaatagc ttcatcaaaa
     3541 ctaatttcat taaaattagt gtttgtgtca tcttttgtgt tgcatgtttt ttcttcactt
     3601 gtaacatcac aagccttttt tgaagttgaa gttgtactct ttgtttgtgt ttgacacgca
     3661 cataatgtaa gacttaaaag tatgagtaaa aattttttca tgcccctaat atacaaataa
     3721 attaaaaaaa agtaaagaaa aaagcttcga tctctcgaaa cttaaaaatc ttagtggggc
     3781 ggctgacgcg attcgaacgc gcgcgtgacg gagccacaat ccgctgtgtt aaccaacttc
     3841 accacaaccg ccataattgc ttaacgcttg tataatatac catgtgattc tcttctttgc
     3901 aagcaaaaaa tgaaaataaa ttaaaaaaag tgcatttttt ttcgtataag agtatatgaa
     3961 ggtaaaagag atggaagtgg agcaacgccc acgagaaaaa gcgttgcgct atggtttaga
     4021 atcattaagc gatctagaat tggttgcgtt gattctgcaa agcggaaata aaaaccgttc
     4081 cgtatttgaa attgcatcgg atgtgttgaa agaaagtgaa ggtttatcaa aattaatgac
     4141 aatgcacgta aatacattga tgcagattca aggaattcgt gaagttaagg ctttacaact
     4201 tttggcaagt gtagaactta gtaagcgtgt tattaaatca aaagtatatc atgctcctat
     4261 tttaaaacca gaagatgtga ttgaatggtt caaatttgag tatggaacta tgtcgcaaga
     4321 atgttttatc gccctttatc tagacacaaa aagcaagcta atttcgcatc gtgttttatt
     4381 taagggaaca ttaaatgaaa gcgtggtaca tccacgtgaa gtatttaagg aggctttttt
     4441 acaaaacgca aattcagtat tgattgcaca taa
//
LOCUS       DBMDWL010000167         4408 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8376_length_4408_cov_2.023682, whole genome shotgun sequence.
ACCESSION   DBMDWL010000167 DBMDWL010000000
VERSION     DBMDWL010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8376_length_4408_cov_2.023682"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="ACZPDN_09845"
     CDS             <1..463
                     /locus_tag="ACZPDN_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1298960.1"
                     /translation="TYAILMGKEEILIKNGKILNPSLLSSGTKSGLDIAGIVASIKSD
                     KHSFYYCDEKFSYVHSDIEKGFLSLMISLLKPNSQLFFTTHNLDILDLPLPKHSFNFL
                     KKEMFEDDQVISVINAADYLKKSTDSLRNAVDNDLFSVSPNLDLLYEIEEL"
     gene            481..696
                     /locus_tag="ACZPDN_09850"
     CDS             481..696
                     /locus_tag="ACZPDN_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298961.1"
                     /translation="MSKNIYHFFVEGEGERAVINTLKTDFQCIQAGKVDVFNVIQNKL
                     NKNILMKLKPGKLLFWCLIQIKMKLIY"
     gene            660..983
                     /locus_tag="ACZPDN_09855"
     CDS             660..983
                     /locus_tag="ACZPDN_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298962.1"
                     /translation="MFDTDKDEIDILDYNLRFLKKQSNIKKVIIIPEVKNLEDELVRS
                     CNIANVKELMKSKSNSEYKSDLARCKNLKQVLTNYQFDINSFWNQNPKGVFVKYKNDS
                     NLIKK"
     gene            complement(1280..1705)
                     /locus_tag="ACZPDN_09860"
     CDS             complement(1280..1705)
                     /locus_tag="ACZPDN_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1298963.1"
                     /translation="MLSIYPACFFEEEENHYSVIFPDFNYLSTCGDSLQDAMKMAVDC
                     LAGALFSAKLDNEDIPIPSKLEEMDPTKIASELEFEFKQSFVTLVSVDVEEYAKTHFN
                     KAIKKTLTIPEWLNKEAISKNINFSQVLQEALIAKLHSN"
     gene            complement(1796..4111)
                     /locus_tag="ACZPDN_09865"
     CDS             complement(1796..4111)
                     /locus_tag="ACZPDN_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR1298964.1"
                     /translation="MWRFSGIFRDVMVYKQPSQHIQDLKIDTNIDLDTKKSILTCTCK
                     MSSNESHILQAKLYDPQGNIVWNGNMQDTFEIPVENTSLWSSEYPNLYQLEIVLYDAN
                     NEIIETIQEQVGFRKFTLEYGIMKLNGKRIVFHGINRHEWNAKTGRAITYEQMEEDIH
                     MLKALNINAVRTSHYPNNSYWYKLCDQYGIYVIDEMNLETHGANDIIPQNKTEWTDCV
                     LDRARSMYERDKNHACILMWSLGNEAGSGSNFMILHDYFKEQDASRLVHYEGITQDRT
                     FENASDIESRMYATPQSIKDYLESHPMKPFMLCEYMHAMGNSLGGMEEYTNLEDEYEQ
                     YQGGFVWDYVDQAIEKDGKYYYGKDFNDYPNDSNFCGDGILLANRQETGKSQELKQLY
                     RYVDMEIHFDSVTIKNKNLFYDLGKDTFIFELKRNGLAIQSGIFKTSVEPGTTKTMPV
                     QFKQINTPGYYTKTIYYKTPLGIQAFDQQVFEVKQDQSKESAMLVVEGQNTIGIQFMD
                     TEYLFDKRKGLVSIQVNNTELLQEAPKPVFYRALTDNDRGAKQTYANWLNASLFQNVI
                     DFKMIRNSKCAQMIYTIQLESIEEECKLVYTVYNNQSIQIQLHLDKNSKRQTLPLFGI
                     EFALKETFKNFAYFGKGEKDTYIDRNHALTDFYSENVDTNYIPYLKPQEHGNHVDCKW
                     CCLSDGTNAFNVKSENMECQASKYSFMHLYNANHTFELPQTHTTYLRITKQQMGVGGI
                     NSWGAKVQPQYLIDQSKNLDFTCTLYISKKD"
     gene            complement(4170..>4408)
                     /locus_tag="ACZPDN_09870"
     CDS             complement(4170..>4408)
                     /locus_tag="ACZPDN_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298965.1"
                     /translation="PEEETEYTMDLKEYTAKRIGTNLAVTKLKQNIKKGEEIDVVQPL
                     NAKTRLETKELELKKGEKIDLTVFSSQEIHTRKR"
BASE COUNT         1202 a          638 c          874 g         1694 t
ORIGIN      
        1 tacttatgcg attttgatgg gaaaggaaga gatacttata aaaaatggta agatattaaa
       61 tccttctctg ttgtctagtg gtacaaaatc tggtcttgat attgcaggga ttgtggcttc
      121 tattaaatcg gataagcata gtttttatta ttgcgatgaa aagttttcgt atgtacattc
      181 agacattgaa aaaggctttt tatctttaat gatttctttg ttgaaaccta atagtcagtt
      241 gttctttaca acacataatt tagacatttt ggatttgcct cttcctaagc atagctttaa
      301 ttttttaaag aaagaaatgt ttgaagacga tcaagtcatt agtgtaataa atgcggctga
      361 ttatctaaag aagagtacgg attcattgag aaatgcggtt gataatgact tgttttcggt
      421 ttcaccaaat ttagatttac tatatgaaat tgaagaatta taagattagg agcaattgtc
      481 atgtctaaaa atatttatca tttctttgtt gaaggtgaag gtgagcgtgc cgttattaat
      541 acattaaaaa cagattttca atgtattcag gctggaaaag tagatgtttt caatgtgatt
      601 cagaataaat tgaataagaa tattttaatg aaattaaaac ctggaaaatt gttattctgg
      661 tgtttgatac agataaagat gaaattgata tattagatta taatctgcgt ttcttaaaga
      721 aacagtccaa tataaaaaag gtgattataa ttcctgaagt gaaaaattta gaggatgagt
      781 tagtacgtag ttgcaatatc gcaaatgtaa aggagctcat gaagagtaaa tcgaatagtg
      841 agtataagag tgatttagca agatgtaaga atcttaagca ggttttaaca aactatcagt
      901 ttgatatcaa ttccttttgg aatcaaaatc caaaaggagt attcgtaaag tataagaatg
      961 atagtaattt gattaagaag taatggtgaa aatatctgtg gattgtcaat aaatgagaat
     1021 cctttagaga taaagtttgg agttgctttt tagtaataat tcaatgaact tattctttgt
     1081 gaaataagaa aatgagctga agtttgattg atacaattca atctgtttga tgaagcaact
     1141 gttgtatcat agtcgagttc tgttacgacc tcaattgaat ttacggtttt tgatggtgct
     1201 ggtagtatca ttatttcttt gatggtaaaa aagaatagtt taaagcccca taccttctat
     1261 agatatgggg cttgatgttt taattggagt gtaattttgc aattaatgct tcttgaagta
     1321 cttgtgaaaa attgatgttc ttgctaatag cttctttgtt taaccattca ggtattgtga
     1381 gtgttttttt gatggcctta ttgaaatgtg ttttcgcata ttcttcaaca tcaacagata
     1441 caagggtgac aaaagattgt ttaaattcga attcaagttc agatgcgatt tttgttggat
     1501 ccatttcttc taactttgaa ggaattggaa tatcttcatt gtctagtttt gcactgaaca
     1561 atgcaccggc tagacaatca acggccattt tcatggcatc ttgtagtgaa tccccacatg
     1621 tagaaagata attgaagtct ggaaaaataa cggaataatg attttcttct tcttcaaaga
     1681 aacatgctgg ataaattgat agcatattgt ttgcctcctc tttaaatagt atattgaaat
     1741 atacgtatat attcaatacg tatatttacg aaacgtgtaa ataaaaagaa ggaaattaat
     1801 ccttcttaga aatgtaaagt gtacatgtaa agtctaagtt tttcgattga tcaatcaaat
     1861 attggggttg tactttagca ccccagctat tgattccgcc tacgcccatt tgttgttttg
     1921 tgattcttaa ataggttgtg tgggtttgtg gcaattcaaa cgtgtggttt gcgttatata
     1981 agtgcataaa ggaatatttg ctggcttgac attccatgtt ttctgacttt acgttaaatg
     2041 catttgttcc atcagatagg cagcaccatt tgcagtcaac atgattgccg tgttcttgtg
     2101 gttttaagta tggaatgtag tttgtatcta cattttcaga gtagaaatcg gttagtgcgt
     2161 gatttcgatc tatatatgtg tctttttcgc cttttccaaa gtacgcaaag tttttaaatg
     2221 tttcttttag ggcaaactca attccaaata gtggaagggt ttgtcttttt gagtttttgt
     2281 ctaagtgcaa ttggatttga atgctttgat tgttgtagac tgtatagacg agtttacatt
     2341 cttcttcaat ggattctagt tgaattgtat agatcatttg tgcgcatttg gagttgcgta
     2401 tcattttaaa gtcaatgaca ttttggaata ggcttgcgtt aagccagttg gcataggttt
     2461 gtttggcacc acgatcattg tcggttaagg ctctgtagaa gacaggtttt ggtgcttctt
     2521 gaagtaattc tgtattgttt acttgaatgg atactaagcc ttttcgtttg tcgaatagat
     2581 attcagtgtc cataaattga atgccaattg tattttgtcc ttccactaca agcattgcac
     2641 tttctttgga ttggtcttgt ttgacttcga agacttgttg gtcaaaagct tgtataccaa
     2701 gtggtgtttt gtagtagatg gtttttgtat agtagcctgg cgtattgatt tgtttaaatt
     2761 gtacgggcat tgtttttgta gttcctggtt ctacacttgt tttaaagatg ccggattgga
     2821 tggcaagtcc atttcgtttg agttcaaaga taaaggtgtc tttgccaagg tcgtagaata
     2881 ggtttttgtt tttaatggtt acagaatcga aatggatttc catatcgaca tatctgtaga
     2941 gttgtttgag ttcttggctt ttgccagttt cttgtcgatt ggctagaagg ataccatctc
     3001 cacagaagtt agaatcgttt ggatagtcgt taaagtcttt tccgtagtag tatttgccgt
     3061 ctttttcaat ggcttggtct acatagtccc atacaaagcc gccttggtat tgttcgtatt
     3121 cgtcttctag gtttgtatat tcttccatgc ctcctagact atttcccatg gcgtgcatat
     3181 attcacatag cataaatggt ttcattggat ggctttctag atagtctttt atactttgtg
     3241 gtgtggcata cattctagat tcgatgtcac tggcattttc aaatgttcga tcttgtgtga
     3301 taccttcata gtggacaagt cttgatgcat cttgttcttt gaagtagtcg tgtagaatca
     3361 taaagttgga tccgcttcca gcttcatttc ctagtgacca cattaaaatg catgcgtggt
     3421 ttttatctct ttcgtacatg cttcttgcac gatctaatac acaatcggtc cattctgttt
     3481 tgttttgtgg gatgatgtca ttagcaccat gtgtttctag attcatttcg tcaatgacat
     3541 agattccata ttggtcgcat aacttatacc aatagctgtt gtttggatag tgggatgttc
     3601 ttacggcatt gatgtttaat gcctttagca tatggatgtc ttcttccatt tgttcatatg
     3661 taatggctct tcctgttttg gcgttccatt cgtggcggtt gatgccgtgg aatacgatgc
     3721 gttttccatt gagtttcata atgccatatt cgagtgtgaa tttacgaaat cctacttgtt
     3781 cttgaatggt ttcaatgatt tcgttgtttg catcgtatag aacaatttct aattggtata
     3841 ggtttgggta ttcactactc caaagacttg tattttctac aggtatttca aatgtatctt
     3901 gcatattgcc attccatacg atatttcctt gtggatcgta tagtttggct tgtagtatat
     3961 gactttcatt actgctcatt ttgcatgtgc atgtaagaat actttttttg gtgtctaaat
     4021 caatatttgt atcgatcttt aagtcttgta tgtgttggct tggttgtttg tagaccatga
     4081 catctctaaa gattccgcta aagcgccaca tgtcttggtc ttctagccat gtgcctgtgc
     4141 atttttgaaa gatgcgtacg cagagtacgt tatctttttc ttgtgtgtat ttcttgagat
     4201 gaaaaaacgg tcaaatcaat tttctcgcct ttttttaatt ccagctcttt tgtttctagc
     4261 cttgtttttg cgtttaatgg ctgaactaca tctatttctt ctcctttttt gatattttgt
     4321 tttagctttg taactgctaa gtttgtcccg atcctttttg cagtatattc tttcaaatcc
     4381 attgtatatt ctgtctcttc ttcaggaa
//
LOCUS       DBMDWL010000168         4396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8404_length_4396_cov_3.071478, whole genome shotgun sequence.
ACCESSION   DBMDWL010000168 DBMDWL010000000
VERSION     DBMDWL010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8404_length_4396_cov_3.071478"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="ACZPDN_09875"
     CDS             complement(<1..1510)
                     /locus_tag="ACZPDN_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1298966.1"
                     /translation="MAGIFGKLFSEEQRELSALEKIADQVLKYEPEMQALSDDELRAK
                     TDEYRKRMADGETVDDLLPEAFATAREAAYRVINEKPYKVQIMGSVAMHKGDISEMKT
                     GEGKTLTATMCVYLNALAGQGVHVITVNEYLAGRDAAWMGEIYRFLGLTVGVNTRDLK
                     PREKRDAYACDITYTTNSELGFDYLRDNMVTEVEDRVMRGLHMAVIDEVDSVLIDESR
                     TPLIISGGKKQTANLYIQSDRFVKTLIAPEYETDKFTHEKTLISGDYDIDEKTRQIML
                     SEDGVHKAEKFFKIKNLYDIEHTQLVHHINQALKANYIMMREVEYVVSDEKEIVIVDQ
                     FTGRMMPGRAYSDGLHQAIEAKEGVPIKEETSTLATITYQNFFRLYEKLAGMTGTAKT
                     EEEEFLSTYNMKVVVIPTNRPVARKDLPDEIYAHKKDKYAALVREVKRLYETGQPVLV
                     GTIAVETSEVISQLLNKEHIPHEVLNAKNHAREAEIIAKAGRPKSVTIATNMA"
     gene            complement(1609..2085)
                     /locus_tag="ACZPDN_09880"
     CDS             complement(1609..2085)
                     /locus_tag="ACZPDN_09880"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HIR1298967.1"
                     /translation="MEKVEIESFTLDHTKVKAPYVRLISEEKGPNGDIVSNFDVRLCQ
                     PNRQEIPTGVMHTLEHLFALYLRPRITGYLDCSPFGCRTGFHLLAWGKHSSKDVAIAV
                     KEALELITTTEWEDVPGTEEKECGNYKDHSLFGAKEWAKEILEKGMSCDPFERKIV"
     gene            complement(2066..3103)
                     /locus_tag="ACZPDN_09885"
     CDS             complement(2066..3103)
                     /locus_tag="ACZPDN_09885"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR1298968.1"
                     /translation="MDLQQGLIPCFFLAMKKFQKDLIDWYIHNHRPLEFRLKKDPYEI
                     WISEIMAQQTRIEAMLPYFKRWIQQLPDIESVAKCDDEKLNKLWQGLGYYSRCKNIKK
                     CAIECVEKYNGKLPCTKEELLKLPGIGPYTAGAIASIANGQRVSAVDGNVIRVFSRLY
                     DVCEDVTKTSVKKQIEELVDESLPSKEEISYYNQAIMELGALICIPKNPRCELCPVKE
                     YCDAKDPGSLPYKAKKKARKIEYKHLYILVHKDKVHVVKRADTGLLAGLYGFDEMKPE
                     KIIESVELKPYIHIFSHIEWHMDATLCIVDKEDINYKKIEEIEEHIALPSAFMPFFKQ
                     IKEIIYGKSRN"
     gene            complement(3082..4044)
                     /locus_tag="ACZPDN_09890"
     CDS             complement(3082..4044)
                     /locus_tag="ACZPDN_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIR1298969.1"
                     /translation="MSIIKLNPAFKDYLWGGTKLKENYGKKTDMDPVAESWELSVHPD
                     GPSVVASGEYEGLSFPEYLEKKGKGVLGTKGNTFEFFPILIKFIDAKQPLSIQVHPDN
                     EYALRVEHEYGKTEMWYILDCEPDSFLYFGVNKEITKEEFERRIQDNTLLDVLKAVPV
                     HKGDVFFIKSGTIHAIGAGIQICEIQQNSNTTYRVYDFGRVGKDGKPRELHIQKAIDV
                     SNLSPIESDFKPCGPEEKNGDCDTVRLASCEYFTTYESTLNGTTTLHVDDESFGSILL
                     IEGSATIENEGTTLEANKGDSVFIDANSGDVKIQGNCQWIYSRV"
BASE COUNT         1320 a          937 c          587 g         1552 t
ORIGIN      
        1 cagccatgtt tgtcgcaatt gtaactgatt ttggacgacc tgcctttgcg atgatttctg
       61 cttcacgagc atggttcttc gcatttaaca cttcatgagg aatgtgttct ttatttaata
      121 attgagaaat aacctcactt gtttctaccg caatcgtacc aactagaact ggttgtcctg
      181 tttcatataa acgttttact tcacgtacca atgcggcata tttatctttc ttatgtgcat
      241 aaatttcatc tggaagatcc ttacgagcta ctggacggtt tgtaggaatc acaacaacct
      301 tcatgttgta agttgataag aattcttctt cttcagtttt tgcagtacca gtcatacctg
      361 ctaatttttc atataaacgg aagaagtttt ggtatgtgat tgttgccaaa gtcgaagttt
      421 cttctttaat tggaacccct tctttagctt caatagcctg gtgtaaacca tctgaatatg
      481 cacgtccagg catcatacga cctgtaaatt ggtcaacgat aacgatttct ttttcatcac
      541 taaccacata ttcaacttca cgcatcataa tatagtttgc ctttaaagcc tggttaatgt
      601 ggtgaacaag ttgtgtatgt tcgatatcat aaaggttttt gatcttaaag aatttttctg
      661 ccttatgaac accatcttca ctcaacatga tttgacgtgt tttttcatca atatcatagt
      721 ctccagaaat caaagttttt tcatgtgtaa acttatctgt ttcgtattct ggagcaatta
      781 aagtcttcac aaaacgatcc gattgaatgt ataagtttgc ggtttgtttt ttaccacccg
      841 aaataatcaa tggtgtacga ctctcatcaa ttaatacaga gtcaacttca tcgattacag
      901 ccatgtgaag tccacgcatt acacgatctt caacttctgt aaccatatta tcacgcaaat
      961 aatcaaatcc cagttcagag tttgttgtat aagtaatatc acatgcatac gcatcacgtt
     1021 tttctcttgg ttttaaatcg cgtgtattta caccaaccgt taaacctaag aaacgataga
     1081 tttcacccat ccacgctgca tcacgaccag ccaaatattc attaactgtg attacgtgaa
     1141 caccttgacc tgcaagagca tttaaatata cacacattgt tgctgttaaa gttttacctt
     1201 caccagtctt catttctgaa atatcacctt tgtgcattgc aaccgaaccc ataatctgga
     1261 ctttataagg tttttcgttg attacacgat atgccgcttc tctagctgtt gcgaatgctt
     1321 ctggaagaag atcatctact gtttctccat ccgccattct ttttctatat tcatccgttt
     1381 ttgcacgcaa ctcatcatcc gataatgcct gcatttctgg ttcatatttt aatacttgat
     1441 ctgcaatctt ttctaatgca gataattcac gttgttcttc tgaaaataat tttccaaaaa
     1501 ttcctgccat aatataagtt ctccttttaa agactatact atattttaac atagaaatga
     1561 cacgataaat agggcaactc aaataaaaaa agagaatttc acattctctt acacaatttt
     1621 tctttcgaac ggatcacaac tcattccttt ttctagaatt tccttagccc attctttagc
     1681 accaaataaa gaatggtctt tatagttgcc acactctttt tcttcagtac ccggaacatc
     1741 ttcccattct gtagttgtga ttagttccaa agcttctttt acagcaatcg caacatcctt
     1801 acttgaatgt tttccccatg ctaaaaggtg aaatcctgta cgacacccaa atggcgaaca
     1861 atctagatac ccagtgattc taggacgaag atataatgcg aacaaatgtt ctaaagtatg
     1921 cataactcct gttggaatct cttgtctatt tggttgacat aaacgaacat caaaattcga
     1981 tacaatatct ccattgggtc ctttttcctc acttattaaa cgaacataag gggcttttac
     2041 ttttgtatga tctaaagtaa agctttcaat ttctactttt tccataaata atctccttta
     2101 tctgtttgaa gaaaggcata aatgcacttg gcaatgcaat atgctcttct atttcttcaa
     2161 tctttttata atttatatct tctttatcta ctatacataa tgttgcatcc atatgccatt
     2221 caatatgcga aaaaatatgt atataaggct ttaattcaac actttctata atcttttctg
     2281 gtttcatttc atcaaaacca taaagcccag ccaataagcc ggtatccgct cttttaacta
     2341 catgtacctt atctttatgc accaatatat ataaatgttt atattctatt tttcgcgctt
     2401 tctttttcgc cttgtaaggc aaactgccag gatctttggc atcacaatac tctttaactg
     2461 gacacaattc acacctagga tttttaggaa tacaaataag agctcccaac tccattatgg
     2521 cttgattata ataggaaatt tcttcttttg aaggaagact ttcatctacc aactcttcaa
     2581 tctgtttctt cacacttgtc ttggtaacat cctcacaaac atcatacaac ctagaaaata
     2641 cacgaatcac atttccatcg actgcactta ctctttgtcc attcgcaatc gaagcgatag
     2701 ctcctgctgt atatggacca atacctggta attttaataa ttcttctttt gtacaaggca
     2761 atttcccatt atatttttca acacattcaa tagcacactt cttaatattt ttgcaacgcg
     2821 aataataacc tagtccttgc cacaatttat ttaacttttc atcatcacac tttgcgacac
     2881 tctcaatatc tggcaactgt tgtatccacc ttttaaagta gggaagcata gcttcaatac
     2941 gagtttgttg ggccataatt tcactgatcc atatttcata aggatctttc tttaaacgaa
     3001 attctagtgg tcgatgatta tgaatatacc aatcaattaa atctttttga aattttttca
     3061 tagccaaaaa gaagcagggg attaaaccct gctgtagatc cattggcaat ttccttgaat
     3121 tttcacatca ccactgttcg catcaataaa tacagaatca cctttatttg cttctaaagt
     3181 tgtaccctca ttttcaattg tagcactacc ctcaatcaac aagatagagc caaagctttc
     3241 atcatcaaca tgaagtgtag tcgtaccatt caatgtagat tcatatgttg taaaatattc
     3301 gcaacttgcc aaacgaaccg tatcacaatc cccattcttc tcttctggtc cacatggttt
     3361 aaagtcagat tcaattgggc ttaaatttga aacgtcaatt gccttttgaa tatgtaattc
     3421 acgaggctta ccatcttttc caacacgacc aaaatcataa acacgatatg ttgtatttga
     3481 attttgttga atttcacaaa tctgaatacc agcaccaatc gcatgaattg taccagactt
     3541 aataaagaaa acatctccct tatgtactgg gactgccttt aacacgtcta ataacgtatt
     3601 atcttggatt cgacgttcaa attcctcttt agtgatttct ttatttactc caaaatataa
     3661 gaatgaatct ggttcacaat ctaaaatata ccacatttca gtttttccat attcgtgctc
     3721 tacacgaagt gcatattcat tatctggatg cacttgaatc gataatggtt gtttggcatc
     3781 aataaattta attaagattg ggaaaaattc aaatgtattc ccctttgttc ctaaaacacc
     3841 cttccccttt ttttctaaat attcaggaaa tgataagcct tcatattcac cactcgcaac
     3901 aacacttggt ccatctggat gtacagacaa ttcccaactt tctgcgacag gatccatatc
     3961 agtcttttta ccataatttt cttttaattt tgttccgccc caaagataat ctttaaaggc
     4021 aggatttaac ttaataattg acattttatg ttagctccct tctagattca ttataaacag
     4081 atggttaaca attgtccact tactaaccac tcacaaaata ggtctgcatc atgtctacat
     4141 cttacattta cttcatatat ttcatttcta ctgtatatat atgaaaaaaa gttaatattt
     4201 caaacatttt atcgaatgtt cttcataatt attgataaag aacgaatgca taacatccac
     4261 tcttctaagc tttcatcaac aactttttaa cagactcttc acttactgag aagatagatg
     4321 cgatagcttt aatttcaaat cccttgtcca tcattttttg atacatttca agctcttttt
     4381 tctcactacc ttgttc
//
LOCUS       DBMDWL010000169         4372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8472_length_4372_cov_2.511590, whole genome shotgun sequence.
ACCESSION   DBMDWL010000169 DBMDWL010000000
VERSION     DBMDWL010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8472_length_4372_cov_2.511590"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="ACZPDN_09895"
     CDS             <1..798
                     /locus_tag="ACZPDN_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1298970.1"
                     /translation="NDDEIAYIAFHLGSALETQKNLTSKVKAVLYCPNYYGINSRITD
                     AIYNDFENDLLITNILTDESQIDKVNDIDLVITTIPLATPIQIPTIRINIILNDRDIS
                     NLRNRISEIQTQKKKNEFEKHLKELLSPELFERKNNLDTHEKCINYMVKKLVKLDYVD
                     THFKDEVLEREKMSSTAFGNFAIPHAMKMHANKTGINILISENPISWNEHPVNLVIMM
                     CFNKNERYIFNEIYDPITMILSEPENVKKILHATDYDDFIKIMVSLL"
     gene            complement(951..2495)
                     /locus_tag="ACZPDN_09900"
     CDS             complement(951..2495)
                     /locus_tag="ACZPDN_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298971.1"
                     /translation="MENIIISKEFLDWYEYYNECKEKYSSLVNDVEDKLICGEYKDIE
                     LPFSNMNTNNLNRDISSLEKIQVLMMQGSTSQINVEIELVKEKMFESCRNVNFGILCN
                     PLASTLYRMILDSSRVKKHIEDVSKRCKENDFYTYVFIPVYLRHFYASICEECIKGYS
                     SYEFVQSVDSLIEEDKFSEACSFCPIYEEIYIQANEKGLLSKELIQVMQQYGVNVPVS
                     KKKVTKQLQVPASVKKKETPKKSKLDIETRKKIVGDGVKKILDMSDEELISRYDGKSE
                     DEVQEMLYEEPWCEDEYFDDDLLAFLSVLTCMSFDFENVFINWLAHFIFIRLLDIHMK
                     VMGEPPKMVKITPPKVQSSKVRKVNQGGTSSNKSVTRSPKIRSKHVSQSSRNVNQARA
                     SVAIKQEETSNGKGGIIFLIIIGLLFGYGYISLKKEEAEWDMAQEQYRIESRRKAEQN
                     EFKVREEKRKKKEGQSSNSSSSSSSSSYDEGSDDAYSGDYDENRYDNDENYRDGVDDM
                     LDELGE"
     gene            2576..3814
                     /locus_tag="ACZPDN_09905"
     CDS             2576..3814
                     /locus_tag="ACZPDN_09905"
                     /inference="COORDINATES: protein motif:HMM:NF037510.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1298972.1"
                     /translation="MNYQNIQSGLSTAFLDKNISSNVLYRPQFISNDYKNGRKVLITI
                     EDELLHCDSFLISVAFITMGGITPLLQTLRTLEDKGIKGKILTTDYLAFSDPKALDKL
                     ATFSNIELKMYLVPDSKNGFHTKGYIFQSNEIYKILIGSSNMTNTALTTNREWNTKIV
                     SIKSGEFVSEMLDEFEELWNHPNTYSYNAFIGAYRVHYEEYKKVKQKEEIKLPSKQLI
                     PNSMQQAFISSLRNIRNSNESKALLISATGTGKTYASAFALQDQNPKKALFLVHREQI
                     AKQALQSYKNVFKDTKTFGLLSGNSKDTNVDYLFATMQTMSKKEVYSSFAPDTFDTII
                     IDEAHRIGAKSYQEIMEYFKPKFWLGMSASPERTDDFDVYAAFDHNIAYEIRLQQALE
                     ENLLCPFHLFWHYRLFNRRK"
     gene            3921..4364
                     /locus_tag="ACZPDN_09910"
     CDS             3921..4364
                     /locus_tag="ACZPDN_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012493.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR1298973.1"
                     /translation="MFCSSKKEAAALSERLNLRGYRTLALSGEDSQEKREDAIDRLVS
                     NTRSDYLQYILTVDIFNEGVDIPEINQVVLLRPTQSPIVFVQQLGRGLRKSQDKEYVV
                     ILDFIGNYKNNFMIPIALSGDRSYNKDNIRRYVLEGESESYLILE"
BASE COUNT         1547 a          850 c          590 g         1385 t
ORIGIN      
        1 aatgatgatg aaatagctta tattgccttt catttaggaa gtgcattaga aacacaaaag
       61 aatttaactt caaaagtaaa agcagtttta tattgtccta actactatgg aatcaattct
      121 agaattacag atgccattta taatgatttt gaaaatgatt tgttgatcac aaacattctt
      181 acagatgaaa gtcaaattga taaagtcaat gacatcgatt tagttatcac aacgatccct
      241 ttggcaactc ctattcaaat tccaacaata cgaatcaata tcatactaaa tgatcgtgat
      301 atctcaaatt taaggaatcg tatttctgaa attcaaacac aaaaaaagaa aaatgaattt
      361 gaaaaacact taaaagagtt attatctcca gaactatttg aaagaaaaaa caatttagac
      421 acacatgaaa aatgtattaa ttacatggta aaaaagcttg tgaagttaga ctatgtagac
      481 actcatttta aagatgaagt attagaacga gaaaaaatgt cttctaccgc atttggaaat
      541 tttgcgattc cacacgccat gaaaatgcat gccaataaaa cgggtatcaa catattaatt
      601 agtgaaaatc caatatcatg gaatgaacac cctgtaaatc tagtcattat gatgtgcttc
      661 aataaaaatg aacgctacat atttaatgaa atctatgatc caatcacaat gattttaagt
      721 gaaccggaaa acgtaaaaaa aatactacat gctacagatt atgatgattt cattaaaatc
      781 atggtatctc tattataaga agtgttgttg cacttctttt tttcatcaac aaaaaaagca
      841 cctatcctaa acagatgcct aaattatcat ttgttaataa atttgtatac tcgtaagtat
      901 actactcatg agtatacaaa tattcaacga ttctttcact gtatttttct ttattctccc
      961 agttcatcca acatatcatc tacaccatca cgataatttt catcgttatc gtaacgattc
     1021 tcatcataat caccagaata tgcatcatca cttccttcat cataagatga ggacgaagaa
     1081 gatgatgatg agtttgaact ttgtccctct ttctttttac gtttttcttc ccttacctta
     1141 aactcatttt gttcagcttt ccgcctactc tcaatacgat actgctcctg tgccatatcc
     1201 cattctgcct cttccttttt caaagaaata tatccatatc caaacagaag tccaataatg
     1261 atcaagaaaa taattccacc cttaccattt gatgtttcct cctgtttaat agctactgaa
     1321 gccctggctt gatttacatt tctagaagat tgactcacat gcttactgcg aatctttggg
     1381 gatctcgtca cacttttatt agatgaagtg ccaccctgat ttaccttacg aacctttgaa
     1441 gattgtactt taggtggagt tatcttcacc attttaggtg gttcacccat cactttcata
     1501 tgtatatcaa gcaatcttat aaatataaaa tgagctaacc aattaataaa gacattctca
     1561 aaatcaaaac tcatacatgt taatacgcta agaaacgcaa gtaaatcatc atcaaaatat
     1621 tcatcctcgc accaaggctc ctcataaagc atttcttgaa cctcatcctc tgacttacca
     1681 tcataacgag atataagctc ctcatcactc atatctaaga ttttcttaac gccatcccct
     1741 acaatctttt tacgcgtttc tatatctaat tttgactttt tcggtgtttc tttcttcttt
     1801 acagaagcag gtacctgcag ctgctttgtc accttctttt ttgaaacagg tacattcaca
     1861 ccatactgct gcattacctg tattaattct tttgaaagca atcccttttc atttgcttgg
     1921 atataaattt cttcatagat tggacaaaaa ctacaagctt cactaaactt atcttcttca
     1981 attaaagaat caacagattg aacaaactca tacgaactat atcccttaat acactcttca
     2041 caaatagacg cataaaaatg tcttaaataa acaggaataa atacataggt ataaaaatca
     2101 ttttctttac atcgctttga aacatcctca atatgtttct taactctact cgaatccaaa
     2161 atcatacgat atagcgtact cgccaatgga ttgcacaata tgccaaagtt cacattccga
     2221 caagattcaa acatcttctc ctttacaagc tctatttcta cattaatctg acttgtactc
     2281 ccctgcatca taagcacttg aatcttttct aagctcgata tatcacgatt caaattattc
     2341 gtgttcatat ttgaaaaagg aagttcaata tctttatatt caccacaaat caatttatcc
     2401 tctacatcat ttactaaact tgaatacttt tctttacact cattataata ttcataccaa
     2461 tctagaaact cttttgaaat tattatattt tccattccct atacctctgg tcttatactg
     2521 ttattataac cttttcgatc tatgatttca ttttaatgct ggtataatgt taggtatgaa
     2581 ctatcaaaat atacaaagcg gattatccac cgcatttcta gacaaaaata tatcttccaa
     2641 cgtattatat cgacctcaat ttatttcaaa cgattataag aatggaagaa aagtattaat
     2701 caccattgaa gatgaactat tacactgcga ctccttttta atcagtgtag ccttcattac
     2761 gatgggaggt attaccccct tacttcaaac acttcgtact ttagaagata aaggcatcaa
     2821 aggtaaaatt ctaacaacgg actatttagc ttttagcgat ccaaaagcat tagataaact
     2881 cgctacattt tcaaatattg aacttaaaat gtacctagta cccgactcaa agaatggctt
     2941 tcataccaag ggatatatct ttcaatcaaa tgaaatctat aagatcctta taggttctag
     3001 taatatgaca aatacagcac ttactacaaa tcgtgaatgg aatacaaaaa ttgtgtccat
     3061 aaaatccgga gaattcgttt ccgaaatgtt agatgaattt gaagaattat ggaatcatcc
     3121 aaatacatat tcctacaatg catttattgg tgcttataga gtgcattatg aagaatacaa
     3181 aaaagtgaaa caaaaagaag aaattaaatt accttctaaa caactgatcc caaattctat
     3241 gcaacaggct tttatttcaa gtcttagaaa cattcgcaat tcaaatgaat cgaaagcact
     3301 tttaatctca gctaccggaa ccggtaagac ctatgcctct gcctttgcac ttcaagacca
     3361 aaatccaaag aaagccctat ttttagtgca tagagaacaa attgccaaac aagctcttca
     3421 aagctacaaa aatgtattta aagatacaaa aacatttggc cttttatctg gaaatagtaa
     3481 agataccaat gtagactact tgttcgcgac gatgcaaacg atgtctaaaa aagaagtgta
     3541 ttctagtttt gcccctgata catttgatac catcatcatt gatgaagcac accgcattgg
     3601 agctaaaagc tatcaagaaa tcatggaata ctttaagcca aaattctggt taggaatgag
     3661 cgcaagccca gaaagaaccg atgactttga tgtatatgca gccttcgacc ataatatcgc
     3721 atatgaaata cgtcttcaac aagctctaga agaaaactta ttatgtccat tccacttatt
     3781 ttggcattac cgacttttta acagacggaa atgaaacgga ttttacggat tttaactatt
     3841 taacatccaa tcaaagagtt gactacatta tcaaacaaac taattattat ggctatagcg
     3901 gagaaagagt caaaggactc atgttttgca gctccaaaaa agaagcggct gcccttagtg
     3961 aaagattaaa tttgagaggc tatagaaccc tagctctaag tggagaggat tctcaagaaa
     4021 aaagagaaga tgcgattgat cgcttagtat ccaatacaag aagtgactat ctacaataca
     4081 tattaactgt agacattttt aatgaaggtg tcgacattcc tgaaatcaat caagttgtcc
     4141 ttttaagacc cacacaatcg cctatcgttt ttgtgcaaca attaggtcga ggattaagaa
     4201 aatcacaaga caaagaatat gttgtcattc tagacttcat tggaaactac aaaaacaact
     4261 ttatgattcc aatcgcccta tctggagatc gttcttacaa taaagacaat ataagacgct
     4321 atgtattaga aggtgaaagt gaatcgtact tgatcctgga atgattcttt ca
//
LOCUS       DBMDWL010000170         4321 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8635_length_4321_cov_1.379384, whole genome shotgun sequence.
ACCESSION   DBMDWL010000170 DBMDWL010000000
VERSION     DBMDWL010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8635_length_4321_cov_1.379384"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            182..1138
                     /locus_tag="ACZPDN_09915"
     CDS             182..1138
                     /locus_tag="ACZPDN_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012708.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1298974.1"
                     /translation="MTEWIEQDKKDENHLYVHLKDPSLLDLSIFDRMSNDDFCLPCML
                     TNEKSASIVYATEGYIPLHDFLSQYVFENEEGYIFLHQLFEQAIASNRNKPVLFDPDY
                     VFVSGFGDRFAFVVVPIQISNWMFQKDMSEKWVEYIAKTMQTTTAFEIPGFLLKFLKS
                     AEFSLPNLVLGLDNIRMLYYPKKFSFFKHRRIQTFKVKEPIQAFHRNQPVESSSEDKT
                     QLLQVQVNFSAYLEIEQDTYALSNEINLVGRAMICDVRLQDISVSLKHAKIVCENDRY
                     YIQDLKSTNKTYLNGKEVKRKMRLKNGMKVRFGDVECEFKEG"
     gene            1110..2456
                     /locus_tag="ACZPDN_09920"
     CDS             1110..2456
                     /locus_tag="ACZPDN_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1298975.1"
                     /translation="MLNVNLKKDRYQILESYNSNVYRIQDELSTCIYILKVCQNYETK
                     RYKHQFKTEIDVLSKLNSFLAPSLVTCFEDEMAQYFVETYIPGMNAKQFMKRYKGFIK
                     RYRLLLDILIVLQALHEIGYLYIDLKLENIIYYQNRFYLIDFNACIENHSRVAIMASK
                     SNCAPELMNLREKDVRCDIYALGSLVHELFGFWMMPIVFLCHQKQERRISHLSTFKTM
                     IVLHMFIHILVVLSLCILSVFLILKSTSPKNVFDAYYYHHNIALFEKAYHESDQKDRL
                     YTWVLNGWILDDVYQNEQASIFLMKEAISTSDATLIQYVYDRVSLKKGSKLETFVMMY
                     LKPNANRVNQCMTLANEGNMLLKDKLDIYNQCLQIAIHEEFKISLTPVRTWLESVDES
                     QLLKYTKEFEDLGCNYLEYLLLLRNKEGEIMEVPVVFVDHLKSNRWNDIYAFWRSL"
     gene            2453..4213
                     /locus_tag="ACZPDN_09925"
     CDS             2453..4213
                     /locus_tag="ACZPDN_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298976.1"
                     /translation="MIWMQDVAYTDYIYWQETNSFINGVASSSQNIELFFDKKVDRDS
                     SLVIYDGKQVDLSHDHIYIDVQKEGVTQLVYILKDVDGYALEQGEKSILLDTMIPDVV
                     VKVKNHNIMDVLPLENKETIHVEILEENLKSVEVYLDDEKMDCEGKEFDIEVTSQNHT
                     LSILCRDVAGNEVERKIDILPIVFPECELKDTLYTKENKMDLKFDGICEMPFYLKVYC
                     GQDLCYSLDLENRDAITVDFTSNGTYTFVLEHKEYPQIKKALEGSIVYSNITPIITLQ
                     PSSKVSNEDVFVGLNWYVPYIKKAYVDVELDGIKDRHPFNEEICLKAVENKDLFYKVF
                     AYAMDAFGNEVTDMVIVRIDKRAPSTSLYLNNEQVLDKKIIKKLPEFEFLIDDNRANM
                     RIEYYINGVLMDMDIEKVFNRMIKDDVLKVKTYTYDALSNFEIKEYEFMFSPDKKIEM
                     VSKSEQILRSDEVATFERIWSVNEKNELVLEKNTKVLTSKLKPKIHYVRKKNKVQIFS
                     KDQIESCFVNGKKIAIQKDVLGHDFVEISLKKSKTTIEVKAKNDSGVTTIKKSEIKKP
                     ILKKSFLKQLWIWIQRVFKL"
BASE COUNT         1557 a          506 c          818 g         1440 t
ORIGIN      
        1 tacgtcgatc caggaaaaag gaagaatatt gagtatggtt tggattaaat aaacgtgaat
       61 ataagttaaa gatttcatca ttgtttcatt ttagacaaaa aagtgcgaaa aatcaaaaaa
      121 ataatgaaag ttttctcgaa aaacctcttt taaaaacgtt taagaatatg gaggtgatga
      181 aatgaccgaa tggattgaac aagataaaaa ggatgaaaac cacttgtatg tacatttaaa
      241 agatccaagt ttattagatt tatccatctt tgatcgaatg tcgaatgatg atttttgtct
      301 tccttgtatg ctgacaaatg aaaaaagtgc atccattgtt tatgcgactg aaggctatat
      361 tcctttacat gattttttga gtcaatatgt gtttgaaaat gaggaaggat atattttctt
      421 gcatcaactt tttgaacagg ctatcgcatc caatcgaaat aaacctgttt tatttgatcc
      481 ggattatgta tttgtttctg gctttggcga tcggtttgcg tttgtggttg tccctataca
      541 aatctcgaat tggatgtttc aaaaagatat gtctgaaaaa tgggttgagt atatcgcaaa
      601 gactatgcaa acgacaactg catttgaaat tcctggcttt ttattgaaat ttttaaagag
      661 tgcagagttt agtttgccaa atcttgtctt aggactggat aatatccgta tgttatatta
      721 tccaaaaaaa ttctcttttt tcaaacacag gcgtatacag acatttaaag tcaaagaacc
      781 catacaagct tttcatagaa atcaacctgt tgaatctagt tcggaagata aaacacagct
      841 cttacaagtt caagtaaact tttctgctta tttagagatt gaacaagata catatgcttt
      901 atcaaatgaa attaatttag tgggaagagc tatgatttgt gatgtcagac ttcaagatat
      961 aagtgtctct ttaaaacatg ccaagattgt ttgtgaaaat gacaggtatt atattcaaga
     1021 tttaaagagc acgaataaaa cgtatttaaa tggaaaagaa gtcaagcgaa agatgcgttt
     1081 aaaaaatgga atgaaagtac gatttggtga tgttgaatgt gaatttaaag aaggatagat
     1141 atcagatcct agagtcttac aattcaaatg tatatcgtat tcaagatgag ttgagtactt
     1201 gtatttatat attaaaagta tgtcaaaact atgaaacaaa gcgatataaa catcaattta
     1261 aaacagaaat cgatgtgctt tcaaagttga attcgtttct tgcacctagt ttagtcacgt
     1321 gttttgaaga tgagatggct caatattttg ttgaaacata tattccagga atgaatgcga
     1381 aacaatttat gaaacgatat aaaggcttta ttaaaaggta tcgcttgttg ttggatattt
     1441 tgattgtttt acaagctttg catgaaattg gatatctcta tattgatttg aaattagaaa
     1501 atattattta ttatcagaat cgtttttatt taattgattt taatgcatgt attgagaatc
     1561 attcgcgtgt tgcgattatg gcatctaaat ccaattgtgc ccctgaatta atgaatcttc
     1621 gtgaaaagga tgttcgctgt gatatctatg cacttggctc tttagtacat gagctatttg
     1681 gattttggat gatgcccatt gtgtttttgt gtcatcaaaa acaagaaaga agaatttctc
     1741 atttatctac ttttaagaca atgattgttt tacatatgtt cattcacatc ttggttgtac
     1801 tttctttatg tattctgtct gtcttcttaa ttttaaaaag tacaagtcca aaaaatgtgt
     1861 ttgatgccta ctattatcat cataatattg ccttgtttga aaaggcatat catgaatcgg
     1921 atcaaaagga tcgtttatat acatgggttt taaatggctg gatcttagat gatgtatacc
     1981 aaaatgaaca agcatccata tttttgatga aggaggcgat ttctacgagt gatgcgacat
     2041 tgattcaata tgtatatgat cgtgtgtctt taaaaaaggg gtcaaaactg gaaactttcg
     2101 ttatgatgta tttaaaaccc aacgcaaatc gtgtgaatca atgtatgact ttggcaaatg
     2161 agggcaacat gttattaaaa gataagcttg atatatataa tcagtgtcta caaatcgcaa
     2221 ttcatgaaga gtttaagatt tcattaacgc ctgtgcgtac ttggttagag agtgtggatg
     2281 aatcacagct attgaagtat acaaaagaat ttgaagattt aggatgtaat tacttagaat
     2341 atttactttt attacgaaat aaggagggtg agatcatgga agtacctgtg gtgtttgttg
     2401 atcatttaaa aagtaatcga tggaatgata tatatgcgtt ttggaggagt ttatgatttg
     2461 gatgcaggat gtagcttata ctgactatat ttattggcag gaaacaaatt cgtttatcaa
     2521 tggtgtagct tcatcgagtc aaaatataga attgtttttt gacaagaaag tagatcggga
     2581 ttctagttta gttatttatg atggaaaaca agtggatcta agtcacgatc atatttatat
     2641 tgatgttcaa aaagaaggtg ttacacaact cgtttatata ttaaaagatg tggatggtta
     2701 tgctttagaa caaggtgaaa aatccattct tttagataca atgattccag atgttgtagt
     2761 taaggtaaag aatcataata ttatggatgt tttgccttta gaaaataaag aaacaattca
     2821 tgttgagatt ttagaagaaa acttaaaaag tgtagaagtg tatttagatg atgaaaaaat
     2881 ggattgtgaa ggcaaagaat ttgatataga agtgacaagt caaaatcata cattaagcat
     2941 tctttgtaga gatgttgcgg gcaatgaagt agaacgaaaa attgatatat taccaatcgt
     3001 atttcctgaa tgcgagttaa aggacacgtt atatacaaaa gagaataaga tggatcttaa
     3061 atttgatgga atttgtgaaa tgccatttta tttgaaggta tattgtggac aagatttatg
     3121 ttattctctg gatttagaaa atcgagatgc aataacagtt gattttactt caaatggaac
     3181 gtatacattt gtgctagaac ataaggaata tcctcaaatt aaaaaagcat tagaaggatc
     3241 gatagtatat tcaaacataa ctcccatcat cactttacag ccatcaagta aagtttcaaa
     3301 tgaagatgta tttgtaggtt taaattggta tgttccatat ataaagaaag cttatgtaga
     3361 tgtagaactc gatggaatca aagatcgtca tccgtttaat gaagaaattt gtttaaaggc
     3421 tgtagaaaat aaagatttat tctataaagt ttttgcgtat gccatggatg cctttggaaa
     3481 tgaagttacg gatatggtta ttgtacgtat tgataagagg gctccatcaa caagtttgta
     3541 tctaaataat gaacaggtat tggataaaaa aataattaag aaattacctg aatttgaatt
     3601 tttgatcgat gacaatcgtg caaatatgcg tattgaatat tacataaatg gtgtattgat
     3661 ggatatggat atcgaaaaag tattcaatcg tatgattaaa gatgatgtgt taaaggttaa
     3721 aacgtacact tatgatgcat tatcaaattt tgaaataaaa gaatatgaat ttatgtttag
     3781 tccagataag aaaatagaaa tggtttccaa aagcgaacag attctacgta gtgatgaagt
     3841 ggctacattt gaacgaatat ggagtgtaaa tgaaaagaat gaattggtct tagaaaaaaa
     3901 tacaaaagtt cttacttcaa aattgaagcc gaagattcat tatgtgagaa aaaagaataa
     3961 agtacaaatt ttttccaaag atcagattga atcttgtttt gtgaatggaa agaagattgc
     4021 gattcaaaaa gatgtgttgg gacatgattt tgtggaaata tcattaaaaa aatcaaaaac
     4081 gaccattgaa gtcaaagcta aaaatgattc gggtgttact acgataaaga aatcagaaat
     4141 taaaaaacca atcttaaaaa aatcatttct aaaacaattg tggatttgga ttcagcgtgt
     4201 ctttaaatta tgatcaagct ttggatatat acaaaggatc attgtgcgtg gtttgatgta
     4261 gattccattc aaaaatcagg atcatcatac gcagaaaaat caagctgcat aaaattaaaa
     4321 a
//
LOCUS       DBMDWL010000171         4298 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8712_length_4298_cov_3.447701, whole genome shotgun sequence.
ACCESSION   DBMDWL010000171 DBMDWL010000000
VERSION     DBMDWL010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8712_length_4298_cov_3.447701"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /gene="pepF"
                     /locus_tag="ACZPDN_09930"
     CDS             <1..1774
                     /gene="pepF"
                     /locus_tag="ACZPDN_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HIR1298977.1"
                     /translation="VKRNEQKVENTWKTEDIFKDEAAFLACLDTCKELGNELCAYDGK
                     LDDASNLLSFLKGYEKLTCLLDDCLGYSSLLSDQDTADAHHQELKGKANALAVEIFTK
                     LSFSDVQIMQIENLESFYISEPILERYRVYLEEVCRLKEHVLSETEEKLMASSSLMQD
                     TPYSIFGMLNNADLKFEDAIDSKGNKHALTTGTFIHMLEQEDEVLRESAYRNFYAGYG
                     SMIHTLAACLNGQTNQLKFNSTARKYASSLECAVDANNVSPRVYEQLIETVHKNIHVL
                     HKYMRVRKEVMKKDKLHMYDLYVPMVNECNVPVSFEQAKEDVLHSIQLYGTEYAKVYE
                     RGLNSRWIDVYENEGKRSGAYSSGQRVHPFVLMNFANSLDTEFTLAHEMGHAMHSYMS
                     TKYQAPLDRHYKIFVAEVASTCNEALLMDYLRKQNSDPKFQAYLINHFMEQFRTTLFR
                     QCMFAEFEKIINEKTANNEVLTSDALNQIYADLNKFYYGEDVIVDDEISMEWARIPHF
                     YMNFYVYQYATGFSAAMALSQKLLHGTQADIEAYLSFLKGGCTKSPVELLRMAGVDMA
                     SPKPIEDAIELFDSLIDEFESIMK"
     gene            1899..2972
                     /locus_tag="ACZPDN_09935"
     CDS             1899..2972
                     /locus_tag="ACZPDN_09935"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR1298978.1"
                     /translation="MNKLEQARTQIDAIDREMAKLYEARLDAVKDVIAYKIENDLPIL
                     DSGREQAVIEKNLQFIQNPDYKEYYESFIKFIMKESKAYQRTLQSHDKVGYCGVEGAF
                     GHMVSEKLFPSNPKVGVSSFEEVFQMVVHSDVQYGVIPFENTNSGLVGEVLDLLMEYP
                     VYIQSIYDQKIEQCLLGVPGSTLKDVEWVYSKDQALSQSKEFLKELNVQTVAYPNTAM
                     AAQYISAQKDVHKAAIGAKENAELYGLKVLATNIQENATNTTRFLVIGLKPQMQGNRF
                     SMVLSVKHESGALAKIIDCIAKNGLNMESIQSRPMKNKPFEYFFFVEIEGDMSKANDC
                     IEQIQSVCESVKVLGAYTLKEEK"
     gene            2975..4201
                     /locus_tag="ACZPDN_09940"
     CDS             2975..4201
                     /locus_tag="ACZPDN_09940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1298979.1"
                     /translation="MNFVKESVNQTPIVDTVFSIVAKAKEAKAKVGSENVVDATIGSL
                     YDEEGTLVALDSVYSSLKNLDNKVLAAYAASFRGNPDFRKKVYDWVLNGNSHLEHEVI
                     ATPGGTGAVGMTLQECLDEGQTVILPEIAWGSYALMAQMHNLNVEKYSLFEEDHFNLN
                     SFKVACKRVMEKQNRLVMVINDPCHNPTGYSLSEEEWKEVISFLNECSKTHAVVLLND
                     IAYIDFSSRQEKAKEYFAQFDQIEKNFVVIVAFSLSKSMTSYGLRCGAAIVLGQEKEA
                     VEQVKIVFEKDARATWSNINNGAMATFVDVLDNHKADYDKERLHYVNLLKERGDIFVK
                     EANEVGLKHYPYKEGFFVTLSMDNETRDKFHEALMENNIFTVKVNLGIRVAICSLSVE
                     KTKGLAKKMKDILDTV"
BASE COUNT         1495 a          555 c          907 g         1341 t
ORIGIN      
        1 tgtgaaacgt aatgaacaaa aggtagaaaa tacttggaaa acagaagata tatttaaaga
       61 tgaagctgca tttctagctt gtttagatac ttgtaaagaa ttaggaaatg agctttgtgc
      121 atacgatgga aaattggatg atgcatctaa cttattatcc tttttaaagg gatatgaaaa
      181 attaacatgt ttattagatg attgtttagg ctattcttct ttgttgtcag atcaagatac
      241 ggcagatgca catcatcaag aattaaaagg taaggccaat gcgttagcgg ttgaaatttt
      301 tacaaaacta tcttttagtg atgtacaaat catgcagatt gaaaatttag aatcattcta
      361 tatttctgaa cccatcttag aaagatatcg tgtatatcta gaggaagtat gtcgtttaaa
      421 agaacacgta ttatctgaaa cggaagaaaa attaatggca tctagttcat taatgcaaga
      481 tacgccttat tcgatttttg ggatgttgaa caatgcggat ttaaaatttg aagatgcgat
      541 tgattcaaaa ggaaataaac acgctttaac tacaggtaca ttcatccata tgttagagca
      601 ggaagatgaa gtattaagag aaagtgcgta tcgtaatttt tatgctggct atggttctat
      661 gatccatact ttagctgcct gtttaaatgg acagacaaat cagttgaaat ttaatagtac
      721 agcacgtaaa tatgcatcga gtttagaatg tgcagtcgat gcgaataatg tttcaccaag
      781 agtgtatgaa caattgattg aaacggtgca taaaaatatt catgttttac ataaatatat
      841 gcgagtacgt aaagaagtta tgaagaaaga taaacttcac atgtatgatc tatatgttcc
      901 tatggtcaat gaatgtaatg ttcctgtttc ttttgaacaa gctaaagaag atgttttaca
      961 ttccattcaa ttgtatggaa ctgaatatgc caaggtgtat gaaagaggat tgaattctcg
     1021 ttggatcgat gtatatgaaa atgaaggaaa gcgttcaggt gcatattcta gtggacaaag
     1081 agtacatcca tttgtgttga tgaactttgc gaattcttta gatacagaat ttactttagc
     1141 tcatgaaatg gggcatgcga tgcattctta tatgtctaca aaatatcaag ctccattaga
     1201 tcgtcactat aaaatatttg tagctgaagt tgcgagtact tgtaatgaag ctttattgat
     1261 ggattatttg cgcaaacaaa actcagatcc taaattccag gcttatttga ttaatcactt
     1321 tatggaacaa tttagaacaa cattgtttag acagtgtatg tttgcggaat ttgaaaaaat
     1381 catcaatgaa aagacagcaa acaacgaagt cttaacaagt gatgcattga atcaaatcta
     1441 tgctgattta aataaattct attatggtga agatgtgatt gtagatgatg aaatcagtat
     1501 ggaatgggct cgtattccac atttctatat gaatttttat gtataccaat atgcgacagg
     1561 atttagtgca gctatggctc tatcacaaaa attgcttcat ggaacacaag ccgatattga
     1621 agcgtactta tctttcttaa aaggaggatg tacaaaatct ccagttgaat tattacgcat
     1681 ggctggtgtt gatatggcaa gtccaaaacc aattgaagat gcgattgaat tgtttgattc
     1741 tttaattgat gaatttgaga gtattatgaa gtagaaagtc gtttgatttt ctacttttag
     1801 tcattttaaa attagtaata aataaatgac taaaaatgat acattcattt tcagaaaaca
     1861 gatgaaaatt atggtatgat attcattgag gtgaacccat gaataaatta gaacaagcac
     1921 gtacacaaat tgatgcgatc gatcgtgaaa tggctaagct gtatgaagcc aggttggatg
     1981 ccgtcaaaga tgtaattgcg tataaaatag aaaatgattt accaattcta gattcgggta
     2041 gagagcaggc tgtgattgaa aagaatctgc agtttattca gaatccggat tataaagaat
     2101 attatgagtc ttttataaag ttcattatga aagaatcgaa ggcctatcaa agaacgttgc
     2161 agtctcatga taaagttgga tattgtggcg tagaaggtgc ctttgggcat atggtgagtg
     2221 aaaagctgtt tccttccaat ccaaaagtgg gtgtttctag ttttgaggaa gtctttcaaa
     2281 tggttgtaca ttcggacgtg caatatggtg tgattccttt tgagaacaca aactctggtt
     2341 tagttggaga agtcttagat ttattaatgg aatatccagt gtatattcag tcaatttatg
     2401 atcaaaagat tgaacaatgt ttacttggag ttccgggcag tacattaaaa gatgtggagt
     2461 gggtctattc aaaggatcaa gcgctatcac agtctaaaga atttttaaag gaattaaatg
     2521 tacaaacggt agcctatcca aatacggcga tggcagctca atatatttca gcacagaagg
     2581 atgtgcataa agcagctatt ggtgctaagg aaaatgcaga gctgtatggc ttaaaggtgt
     2641 tggctacaaa cattcaagaa aatgcgacta acacaacgcg attcttagta attggattaa
     2701 agccacaaat gcagggcaat cgctttagta tggttttatc ggttaaacat gaaagtggtg
     2761 ctttggctaa aatcattgat tgtattgcca agaatggatt gaatatggaa agtattcagt
     2821 ctagaccgat gaaaaataag ccttttgagt atttcttctt tgttgaaatt gaaggggata
     2881 tgtcaaaggc aaatgattgt attgaacaga ttcaatctgt atgtgaaagt gtaaaagtat
     2941 tgggagcata tactttaaag gaggaaaaat aagtatgaat tttgtgaagg aaagtgtcaa
     3001 tcaaacacca atcgtcgata ctgtattctc aattgttgcc aaggcaaaag aggctaaagc
     3061 taaggtggga agtgaaaatg ttgtcgatgc gacaatcggt tctttatatg atgaagaagg
     3121 tactttggtt gccttggata gtgtatatag ttcattaaag aatttagata ataaagtatt
     3181 ggcagcgtac gcagctagtt ttagaggaaa tccagacttt agaaaaaaag tatatgactg
     3241 ggtattgaat ggaaattcac acttagaaca tgaagtgatc gcaacacctg gaggaacggg
     3301 tgctgttggt atgactttac aagagtgttt agatgaaggt caaaccgtaa tcttgccaga
     3361 aatcgcatgg ggatcttatg cattaatggc acagatgcac aatttaaatg tggaaaagta
     3421 tagtttattt gaagaagatc actttaattt aaattctttt aaagtggctt gtaaaagagt
     3481 gatggaaaaa caaaatcgct tagtgatggt aatcaacgat ccttgtcaca atccaacagg
     3541 atattcttta agtgaagaag aatggaaaga agtgatctct ttcttgaatg aatgttcaaa
     3601 aacacatgct gttgtattgt tgaatgatat tgcgtatatt gatttttctt ctagacaaga
     3661 aaaggcgaaa gaatactttg cgcagtttga tcaaattgag aaaaactttg tcgtgattgt
     3721 cgcatttagt ttatccaaat caatgacaag ctatggactt cgttgtggag ctgccattgt
     3781 gttaggacaa gaaaaagaag cggttgaaca agttaaaatc gtatttgaaa aggatgcacg
     3841 tgcgacttgg tcaaatatca acaatggagc tatggctaca tttgtggatg tattagacaa
     3901 tcataaagca gattacgata aagaaagatt gcattatgta aatttattaa aagaacgtgg
     3961 agatatcttt gtcaaagagg ctaacgaagt gggattaaaa cattatccat ataaagaagg
     4021 tttctttgta actttatcta tggataatga aacgcgtgat aaattccatg aggcattaat
     4081 ggaaaacaac atttttactg tcaaagtaaa tttgggtatt cgtgttgcga tttgttcttt
     4141 atctgttgaa aagacaaagg gattggcaaa aaagatgaaa gatattttgg atacagtata
     4201 aaatggtacc cataaggagg acaacaacgt gacagttgtg atactcactt gtgggtatct
     4261 tttttcgttg ttttaaaata aatatgtcaa gagccaaa
//
LOCUS       DBMDWL010000172         4246 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8877_length_4246_cov_1.650422, whole genome shotgun sequence.
ACCESSION   DBMDWL010000172 DBMDWL010000000
VERSION     DBMDWL010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8877_length_4246_cov_1.650422"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="ACZPDN_09945"
     CDS             complement(<1..631)
                     /locus_tag="ACZPDN_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1298980.1"
                     /translation="MIKQYKFAICDDDIQFCNKIKSYIIEYYGENKAEISIFNSGEDF
                     IKSKVKYDCIFLDIEMINLNGIEIATMIRNYDLDTYIIIVSGFPKYKNVAYRLHVFDY
                     LDKPLDKTMLFKTLDDMTILLQNSQIDIYEYFQTNHGLVKLKVDDIMYFEYSERKINL
                     YTNSDSIYQFYDTIYKLEERFKQYHFISPHKAFLVNIDYIQNIHLNDLIL"
     gene            complement(1204..1488)
                     /locus_tag="ACZPDN_09950"
     CDS             complement(1204..1488)
                     /locus_tag="ACZPDN_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298981.1"
                     /translation="MNDKQKNIIDKLNKDNESLNQGHKSMVEYSKGMICIGLFVVCFL
                     IYYFTKNIFFLLGFLSFEIFNLIYAKNDKKKIGIGIIVGVVIYILLELWI"
     gene            complement(1494..1772)
                     /locus_tag="ACZPDN_09955"
     CDS             complement(1494..1772)
                     /locus_tag="ACZPDN_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298982.1"
                     /translation="MKKNRLQRLQEDMNEDLQKYLDEDLLDTTPKEKKESSFWMKCVA
                     LCLLCSYGIAQFLGRVSGDKQAIVQYGLSGIIVICALAFYIWLRRKYR"
     gene            complement(1774..2073)
                     /locus_tag="ACZPDN_09960"
     CDS             complement(1774..2073)
                     /locus_tag="ACZPDN_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298983.1"
                     /translation="MRMSVLINKFKGIRYFIKNGIFNEERAIEISKFDHNTIEALVHS
                     QLLVQVDGRVYLDKPLYDYRYKEKYIYYGEIILLIILLIFAFHVSYLKTTLFRRW"
     gene            complement(2048..2200)
                     /locus_tag="ACZPDN_09965"
     CDS             complement(2048..2200)
                     /locus_tag="ACZPDN_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298984.1"
                     /translation="MSFPKWGLISMVVLLVGGTVYGLYIHDYLQAMMCVVDIGFFVYA
                     YERFNK"
     gene            complement(2187..2369)
                     /locus_tag="ACZPDN_09970"
     CDS             complement(2187..2369)
                     /locus_tag="ACZPDN_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298985.1"
                     /translation="MKKRQRKKNNKNVFIPLIPVEMTEVEVKQILKTRGEDKSKDRRN
                     MHHSTCDGVHVSNELS"
     gene            complement(2374..2505)
                     /locus_tag="ACZPDN_09975"
     CDS             complement(2374..2505)
                     /locus_tag="ACZPDN_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298986.1"
                     /translation="MTLDWLVYVLGVVVVVFLVGMFFKYKKHNRYADKFEDTYPDAK"
     gene            2762..3277
                     /locus_tag="ACZPDN_09980"
     CDS             2762..3277
                     /locus_tag="ACZPDN_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298987.1"
                     /translation="MYLYTYNKKKFGLKINSKNHKNKVVPIHAKEFDVVDQLRFECMK
                     EELKIVQKSALDFVRNILRDIDQNLINHDLEHSISFSQKYPVGTVLSCTYNESEEYMY
                     LYTINDIHYAIYLEDACEGVYIVEPIILYDYKPNGELVDEDTKEILTGILETTQRLNT
                     YNCTKALLEQL"
     gene            complement(3295..4038)
                     /locus_tag="ACZPDN_09985"
     CDS             complement(3295..4038)
                     /locus_tag="ACZPDN_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HIR1298988.1"
                     /translation="MNRIKTEVKEVSVLLRSIPSLTMTLFIVSVITMNLLANKSINLP
                     FSFMALDCGIVVSWLSFLCMDVITKHFGPKAATQASLVAVLVNLGMCLVFFLASKIPG
                     VWGESYVEGSEAIINGALDGTIGGTWYVLFGSTIAFIVSAIVNNYTNSMLGKISNNNS
                     NFKSYAFRSYVSTGIGQFCDNLTFALIVSHQFFGWTLVQCVVCAFTGMIVETLCEVIF
                     SYPGFKILQNWKKNKVGREYFEYMKESSR"
BASE COUNT         1484 a          745 c          527 g         1490 t
ORIGIN      
        1 ctaaaattaa atcgttcaaa tggatgtttt gaatatagtc tatattgact aaaaaagctt
       61 tatgtggact aataaaatgg tactgtttaa atctttcttc caatttatag atggtatcat
      121 aaaattgata gatactatca ctatttgtgt acagatttat tttacgttca ctatattcaa
      181 aatacatgat atcatctacc tttaatttta ctaagccatg gtttgtttga aaatattcat
      241 agatatctat ctgactattt tgtaatagta tagtcatatc atctaaagtt ttaaataaca
      301 ttgttttatc cagtggttta tcaagataat caaatacatg caatctatag gctacatttt
      361 tatatttagg aaatccagaa actattatta tatatgtgtc taagtcgtag ttacgaatca
      421 tagtcgcaat ctctatacca ttcaagttga tcatttcaat atctaaaaaa atacagtcat
      481 atttaacttt tgatttaata aaatcctctc cggaattaaa aatagatatt tccgccttgt
      541 tttctccata atattcaata atatatgatt taatcttatt gcaaaattga atatcatcat
      601 cacaaattgc gaatttatac tgctttatca tttataacac ctactttaca tcattatatc
      661 agatttgaaa tcaacctttt acaaaattgt gtataacact tttcaaaaag ataaatagca
      721 agaaaaagta tccaagattc cgttgtttta agatagaagc ggacaccatt atatatataa
      781 gcagtaaaca tatgtaaatt tctactataa tgttatgtaa acctatagac gtataattct
      841 atcgcatgaa tttacaaagg tcatgtcgag agtcactata attaattctt ttccaaattc
      901 atttaattct cttaatattt taaaaactat atctctattt ctatcttttt ttatttacgt
      961 ccaatccctt ctaaaaatac tgcaacaaaa agataggaaa tgttcaccct aaaagtgttt
     1021 aaaggatatc tctttctcag agctaaaatc cctatgactt ttaaaggttg atttatccct
     1081 atccagtttc atatattcac cacatttacc aaagattgca cctagcattt gcaaggtgct
     1141 ttttttatat cctttaaaaa gaacaaacga tccatctttt atattggatt cactccaata
     1201 tgattatatc cacaattcca aaagaatata gattacaact ccaactataa taccaattcc
     1261 aatcttcttc ttatcattct ttgcgtagat caaattaaag atttcaaatg ataaaaatcc
     1321 aagcagaaaa aatatattct tcgtaaaata gtaaatcaag aaacacacca caaataagcc
     1381 tatacaaatc attcctttag aatattccac catggattta tgtccttgat ttaatgattc
     1441 attatcctta ttcaatttat caataatgtt tttctgttta tcattcataa tcattaccta
     1501 tactttcttc gtagccaaat atagaaagcg agtgcacaaa tcacgattat acccgaaagt
     1561 ccatattgca ctattgcctg tttatcacct gatactcttc ctagaaattg tgcaattcca
     1621 taactacata ataagcataa agctacacac ttcatccaaa agctagattc cttcttttct
     1681 tttggagttg tatctaacaa gtcttcatct aaatattttt gaagatcttc attcatatct
     1741 tcctgtaatc tttgaagtcg attctttttc atatcaccac ctacggaaca atgtagtttt
     1801 taaataagat acatgaaacg caaatattaa gaggatgatc aacaatatga tttctccata
     1861 atagatatat ttttctttgt atctatagtc atacaatggt ttatccaaat atacacgacc
     1921 atctacttgt actaataatt gagaatgtac taaagcttct attgtattat gatcaaattt
     1981 ggaaatctca attgcccttt cttcattgaa tattccattc ttaatgaaat atcgaatacc
     2041 tttgaattta tttattaaaa cgctcatacg catacacaaa aaatccaata tctacaacac
     2101 acatcattgc ttgtaagtaa tcatggatat acaacccata aacagttcca cctacaagta
     2161 gaacaaccat agaaatgagt ccccatttag gaaagctcat ttgaaacatg aactccatca
     2221 caagtactat gatgcatatt acgcctatcc ttggatttat cctcccctct agttttaaga
     2281 atttgcttaa cctctacttc cgtcatttca acaggaatca acggaataaa gacattttta
     2341 ttattctttt ttctctgtct ctttttcata atcttacttt gcgtctggat atgtgtcttc
     2401 aaatttatcg gcatatcgat tgtgcttttt atacttgaag aacataccaa ctaagaaaac
     2461 aacaactact acacctaata cgtaaactag ccaatctaat gtcatcttac tatatcctcc
     2521 ttttctaatt tcatattaac atgtctgtaa tcgttttcat actactttta tttcattatc
     2581 aatcaaactt gtttatatca cattacttaa acaagtttga ttcacttttt aaaacaatct
     2641 atgttttaga catcgataat gtcaattaag atgatatggt tggcttgaat gagggcttga
     2701 acataaatta gaatattccg taggaaccat tctagaaagt cgccttgatg aaattaaata
     2761 tgtgtattta tatacctaca acaagaaaaa atttggttta aaaatcaatt ctaagaatca
     2821 taaaaacaaa gttgtaccaa tccatgccaa agaattcgat gttgttgatc agttgcggtt
     2881 tgaatgcatg aaagaagagt taaagattgt tcaaaaaagc gctctggatt ttgtacgaaa
     2941 tattttaagg gacatcgatc aaaatttaat taatcatgat ttggaacatt ctatttcatt
     3001 tagtcaaaaa tatccagtcg gcactgtact atcttgcaca tacaatgagt ctgaagaata
     3061 tatgtattta tatacaatta atgatataca ctatgcgatt tatttagagg atgcttgtga
     3121 aggtgtttat attgttgagc caattatcct atatgactac aaaccaaatg gtgaattggt
     3181 cgatgaagat acgaaagaga tattaacagg catcttagaa acaacgcaaa gactaaatac
     3241 ctataattgt acaaaagcat tattagaaca actttaagca aaagaaaaag cagtttaacg
     3301 actgctttct ttcatatatt caaaatattc tctacctact ttattcttct tccagttttg
     3361 tagaatcttg aaaccaggat atgagaagat gacttcacat agtgtttcta caatcatacc
     3421 tgtaaaggca cacacaacac attgtaccaa tgtccatcca aagaattggt gtgacacgat
     3481 caatgcgaac gttaggttgt cgcaaaactg accaatacca gtagatacat aagagcggaa
     3541 tgcatacgat ttaaagttgc tgttgttgtt agaaatctta cctaacatgg aattcgtata
     3601 gttattcaca atggcagaaa caataaacgc aattgtactt ccaaatagta cataccaagt
     3661 accaccaatt gttccgtcta atgccccatt aataatggct tctgatcctt ctacatagga
     3721 ttcaccccat acgccaggaa tctttgatgc caagaagaag actaggcaca tacctaaatt
     3781 caccaacacc gctacaagtg atgcttgtgt tgcagcttta ggaccaaaat gttttgtgat
     3841 gacatccata cataagaatg aaagccagct taccacaatt ccacaatcta gtgccataaa
     3901 gctaaatggc aagttaatac ttttgtttgc caaaagattc atagtgatga ccgaaacaat
     3961 aaaaagtgtc atcgtaagtg aaggtatact tcttagtaat acactcactt ctttaacttc
     4021 cgttttaatt ctattcattt tttaccctcc tgttttttta aagatggttg tgaagcaaaa
     4081 catctttgtt caaaccatat aagtctaata tgtttttaat tatttgtcaa agtttgtatc
     4141 acttattagt gattacgcat gaaataatct tcaatttttc atgtttgaag attatttatt
     4201 tttttatata ctttaatagt gtattggtgt acactattat tatgaa
//
LOCUS       DBMDWL010000173         4229 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8932_length_4229_cov_2.162306, whole genome shotgun sequence.
ACCESSION   DBMDWL010000173 DBMDWL010000000
VERSION     DBMDWL010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8932_length_4229_cov_2.162306"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(4..1146)
                     /gene="rsmB"
                     /locus_tag="ACZPDN_09990"
     CDS             complement(4..1146)
                     /gene="rsmB"
                     /locus_tag="ACZPDN_09990"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR1298989.1"
                     /translation="MRKWIWKALDEVVNKDVYSNLYLRNHLHEVDEKDRALATRIFYG
                     TIQNYRYCKACWSKYVKNKLNPKMDVLLTMSTYQLLFLDKVPSYAIVNDAVNIAKKIN
                     VKYAGLTNAVLHKVRPIETDNMALKYSLPDWLYKMWTSQYGQEKALVMASASVNILPM
                     YVRRNVLNTNESDFDLDMFVRVQDPLYIYKGNDYFHHDYYQKGYMSAQDEGSFEIAKF
                     VDPQENERILDCCAAPGTKSMAMAEMMHNKGHIDAYDLHAHRKDLIENDAKRLGMDII
                     HAGVQDATEFKSSNMYDRILCDVPCSGYGVLSRKPDIKLHMVPEDMDTLIPLQYSILN
                     NVCQYVKVGGTLVYSTCTMNKKENEKQIEKFLKVHKEFSLVEEKTI"
     gene            complement(1148..3241)
                     /gene="priA"
                     /locus_tag="ACZPDN_09995"
     CDS             complement(1148..3241)
                     /gene="priA"
                     /locus_tag="ACZPDN_09995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1298990.1"
                     /translation="MLINVYVEYNRLRNTFTYSCEQDVEVGCRVRVEFNNRTLVGFVE
                     EIEVESDFKNIKPVIEVIDEKPLLNNELKLLANYMADFYASSKVTCYKTMLPPALRPS
                     SKHSKIIYEDYAVLKESNETLTSRQKEVLEMFNYPCKMSELRKVSPSITKVLLEKGYI
                     GIEKRIKDSNTHVTSISDVQHELTIEQKDAISKITSTEKMVSLLHGVTGSGKTEVFLR
                     LAENVLSCGKQVLFLVPEIGLTPMMIQRVQARFKQKIAIYHSGLNAQEKLEQYNLVKD
                     HKVDIVVGTRSACFMPFSSLGLILMDEEHDSSYKQDNTPRYHTRDIVLFRAAYHKCKV
                     ILASATPSLESYARAYKNVYELIELKNRIHEMPKICLVDMKKERITNGLSETLLQAIH
                     KRLSKNEQVILLLNRRGYLPVVRCSDCGHVRICPDCGVALTYHKNQNALVCHCCGRQF
                     KFMEECPSCHSKNYFKAGMGTERLEEQIVEIFKDHHVVRMDADSTRKKNAHEKLLQEF
                     EESGDLLIGTQMVAKGLDFERVTLVGILNADATLCRNDYRASETAYEMLEQASGRSGR
                     GKLKGEVMIQTFDPDHFVMQCVKMHDYKRFFNQEMRYRHLGMYPPYVYLCTLIFTHFD
                     FNEAYKQASAAKEYFSSLRVLGPIEIRMQQKKHRVRLILKSKDDQLLHKIVRNYLDET
                     KYVVDVNMHPIMMEE"
     gene            3314..3466
                     /locus_tag="ACZPDN_10000"
     CDS             3314..3466
                     /locus_tag="ACZPDN_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298991.1"
                     /translation="MYIFIGILNILCGILVCFVIGPFDNKVLNICISLLFFVNGILDI
                     RKAIKN"
     gene            complement(3505..3702)
                     /locus_tag="ACZPDN_10005"
     CDS             complement(3505..3702)
                     /locus_tag="ACZPDN_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR1298992.1"
                     /translation="MPQIIPIKELKNTTEITDLCHKTNELTLNKLEMYQDIAISEYQI
                     DIGETKDAKLAVQEMRKKYGL"
     gene            3935..>4229
                     /locus_tag="ACZPDN_10010"
     CDS             3935..>4229
                     /locus_tag="ACZPDN_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298993.1"
                     /translation="MKTIQRTLGIFIVLFSILVMPVQAKENSSSSWMESIAGDTKLSE
                     LSIPGTHDSATQYLSLSPIFQCQDTDIQTQLENVYRYLDIRLVLKNDNLILKHN"
BASE COUNT         1335 a          845 c          561 g         1488 t
ORIGIN      
        1 ttactagatt gttttttctt ctactaaact aaactcttta tgcaccttca aaaacttctc
       61 aatctgtttt tcattctctt ttttattcat tgtacaagtt gaataaacta aagttccccc
      121 aacctttaca tattgacata cattattcag aatagaatat tgtaaaggaa tcaatgtatc
      181 catatcttct ggaaccatat gtaatttaat atctggtttt ctagatagta caccataacc
      241 tgaacaagga acatcacata agatacgatc atacatatta gagcttttaa attcagtcgc
      301 atcttgaaca ccggcatgta taatatccat tcctaagcgt ttggcatcat tttcaatcaa
      361 atctttacga tgtgcatgta aatcgtacgc atcaatatgc cctttattat gcatcatttc
      421 ggccatggcc atactttttg tccctggagc cgcacaacaa tctagaatcc tttcattttc
      481 ttgcggatct acaaacttcg caatttcaaa actcccctca tcttgtgcgc tcatatagcc
      541 tttttgatag tagtcatgat gaaaataatc atttccttta tagatgtaca atgggtcttg
      601 aacacgaaca aacatatcca aatcaaaatc cgattcgttt gtattcaaca catttcggcg
      661 aacatacatt ggcaaaatat tcacactagc actagccata acaagcgctt tttcttgacc
      721 atattgactt gtccacatct tgtatagcca atctggcaaa gaatatttta aagccatatt
      781 atccgtttca atcggccgca ctttatgtaa tacagcattt gtcaaaccag catatttaac
      841 gttgatcttt tttgcgatat tgaccgcatc attaacaatc gcatagcttg gtactttatc
      901 caaaaacaac aattgatatg tactcattgt aagtaataca tccatttttg gatttaattt
      961 attcttcaca tactttgacc aacacgcttt acaatatcga taattttgaa tcgtaccata
     1021 aaagattctt gttgccaaag cacgatcttt ttcatccact tcatgcaagt gatttcttaa
     1081 atataaattg gaatatacat ctttatttac cacttcatcc aaagctttcc atatccactt
     1141 tcgcattcta ttcctccatc ataattggat gcatattcac atccacaaca tattttgttt
     1201 catccaaata atttcgcaca attttgtgta ataattgatc gtcctttgat tttagaatca
     1261 agcgcacacg atgcttcttt tgttgcatac gaatttcaat gggccctaaa acacgcaaag
     1321 aagaaaaata ttctttcgca gcacttgcct gtttataagc ttcattaaaa tcgaaatgtg
     1381 taaagattaa agtacataaa tatacataag gtggatacat acctaggtgg cgatagcgca
     1441 tttcttgatt aaagaacctc ttataatcat gcatctttac acattgcatc acaaaatgat
     1501 cgggatcaaa agtctgaatc ataacttcac cttttaattt tcctcgacca cttcttccac
     1561 ttgcctgttc taacatttca taagccgttt cacttgctct atagtcatta cgacaaagcg
     1621 tggcatctgc atttaaaatc ccaactaaag tcactctttc aaaatcaagt ccttttgcga
     1681 ccatttgcgt accaatcaat agatcgccag actcttcaaa ttcttgtaac aacttttcat
     1741 gcgcattctt ttttcgcgta ctatccgcat ccatacgaac gacatgatga tctttaaaaa
     1801 tttcaacaat ctgttcttcc aatcgctctg tacccatacc ggccttaaaa tagttcttag
     1861 aatgacaact tggacattct tccataaatt taaattgtct tccacaacaa tgacaaacta
     1921 aggcgttttg atttttatga tacgttaagg ctactccaca atctggacat atacgaacgt
     1981 gtccacaatc gctacaacga acaacaggaa gataccctct tcgatttaaa agtaagatca
     2041 cttgttcatt tttagaaagc cgcttgtgaa tggcttgaag taaagtttca gataggccat
     2101 ttgtgattct ttcctttttc atatctacca aacaaatttt tggcatttca tgaatacgat
     2161 ttttcagttc aatcaattca tatacattct tataagctct tgcgtacgat tctaatgaag
     2221 gcgtcgcact tgccaagata actttacact tatgataagc cgcacgaaat aaaacaatat
     2281 cccgcgtatg atagcgtggt gtattgtctt gtttgtagct tgaatcatgt tcttcatcca
     2341 ttaaaatcaa acctaaagaa gaaaacggca taaaacaagc actacgtgtt ccaaccacaa
     2401 tatcgacttt atgatctttt actaaattat attgttctaa tttttcctgt gcattcaacc
     2461 ctgaatgata gatcgcaatt ttttgtttga aacgcgcctg cactctttgt atcatcatcg
     2521 gcgtcaaacc aatttcagga acaagaaata gaacttgttt tccacaagac aatacatttt
     2581 cagctaaacg caaaaagact tcggttttac cagatcccgt tacaccatga agcagtgata
     2641 ccatcttttc tgtacttgtg atcttagaaa tcgcatcttt ttgttcgata gtcaattcat
     2701 gttgtacatc tgaaatactc gttacatgcg tatttgaatc tttaattctt ttttcaatcc
     2761 ctatatagcc cttttctagt aaaacctttg tgatggatgg acttactttt ctaagttcac
     2821 tcatcttaca tggataatta aacatttcaa gcacttcttt ttgacgagaa gtcaatgttt
     2881 cattcgactc ttttaaaacc gcataatctt catagatgat ttttgaatgc ttactagaag
     2941 gacgcaaagc tggtggaagc attgttttat aacaagtcac tttcgaactc gcataaaaat
     3001 cagccatata atttgccaat aactttaatt cgttattcaa taaaggcttt tcatctatga
     3061 cttcaataac aggtttaata tttttaaaat cgctttctac ctcaatttct tcgacaaatc
     3121 ctacaagcgt tcgattgttg aattcaacac ggactctaca tccaacttca acatcttgct
     3181 cacaagagta agtaaaggta ttgcgtaggc gattgtattc tacatataca ttaattagca
     3241 tgcatctatt ataacatcat tcttgatttg acatttatat ttattttagt atatttggca
     3301 taaggagata ggtatgtata tttttattgg aattttaaac attctttgtg gcattctcgt
     3361 ttgttttgtc ataggaccct tcgacaacaa agtattgaat atttgtatat ccttattatt
     3421 ctttgtgaat ggtatcttag acattcgaaa ggccattaaa aactagtaaa agcaccaaaa
     3481 cggtgctttt atacttttat cctattataa accatacttc tttctcattt cttgaacggc
     3541 tagttttgca tcttttgtct ctccaatatc aatttggtat tcagaaatcg caatatcttg
     3601 atacatttca agtttattta aagttaattc attcgtttta tggcataaat cggttatttc
     3661 agtagtattt tttaattctt taatcggtat tatttgtggc atattatctc ccttttatac
     3721 ctttatcata ccacaattat cctatcgttt aggaatcgaa acccaggata aatatctttt
     3781 gtatataaaa tattcaatac tgcttgttct ttcttattca caattattag attacaaaag
     3841 atttatgaat gaatatacaa actgaaattc aatctttgca aaatatgtta cactattctt
     3901 aagcatacac acatacttaa aggaaaataa tattatgaaa accattcaaa gaacacttgg
     3961 gatttttata gtcctctttt ctatacttgt catgccagtt caggcaaagg aaaattcatc
     4021 atcaagctgg atggaaagta ttgcaggaga tacaaagctt agtgaattat cgattccagg
     4081 tacccatgat tcagctacac aatatttaag tttaagtcca atctttcaat gtcaagatac
     4141 agacattcaa acacaattag aaaatgtcta tcgctattta gacatacgat tggttttaaa
     4201 gaatgataat cttatcttaa aacacaact
//
LOCUS       DBMDWL010000174         4214 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_8973_length_4214_cov_1.962315, whole genome shotgun sequence.
ACCESSION   DBMDWL010000174 DBMDWL010000000
VERSION     DBMDWL010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8973_length_4214_cov_1.962315"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="ACZPDN_10015"
     CDS             <1..295
                     /locus_tag="ACZPDN_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298994.1"
                     /translation="AIIGIDGSQMMTAPANYDGDDLELKMYELSETLEYNAEFYSWTY
                     NMNQSQEPVNTDTESIKMSPQYRFIYYKATVQHKTIVVEYVLSKVLKEYKESK"
     gene            315..1130
                     /locus_tag="ACZPDN_10020"
     CDS             315..1130
                     /locus_tag="ACZPDN_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298995.1"
                     /translation="MKKYFALLLSFVLAFSLTACSDAETEKAKSSFEETASIVEKNNE
                     TINKDVKALQQLTKSKVKPLDDTVLKTARETISQAKQQIVEVPECPSNKEDIKEANKK
                     LKKKADKAEIIQALTDSKKAMKDSIAQRKQVTNPSESFVLERLNGIPNVSQALAVNEE
                     NDVNGMLHKAGGYTSAIFFTSDLVDTAANYIEDGDSIEKGTDGGGCIEVFETEEDAQK
                     RDTYLSTFDGGGMLCSGSHKVVGTVIIRTSNYLTATQQNDITTNIMNSLIALK"
     gene            1556..2032
                     /locus_tag="ACZPDN_10025"
     CDS             1556..2032
                     /locus_tag="ACZPDN_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298996.1"
                     /translation="MHRYYQKNLKKWHKTLNSYMNYISYELESLTGQSYDTLIQEIWT
                     NYQEEMLERFPYIGGDAASGTKNLIGAYMFVSMGEVLKKYGVTTAQSGHLMILIYERQ
                     FKKIPSFLRSIMGKIFTNPRFLTKIFLKKDRKNALNAAQNPGSFETQTQIPPEKGV"
     gene            1980..2270
                     /locus_tag="ACZPDN_10030"
     CDS             1980..2270
                     /locus_tag="ACZPDN_10030"
                     /inference="COORDINATES: protein motif:HMM:NF025559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR1298997.1"
                     /translation="MPEVSRHRHRFHQKRGYDFSYHNLVCPLADFAKKYGYEEYMPYL
                     CNLDYVMFGILGAPLYREHTCFDDDYCDFKLKLSAKPMDYDPPVFTQANGYK"
     gene            complement(2491..3204)
                     /locus_tag="ACZPDN_10035"
     CDS             complement(2491..3204)
                     /locus_tag="ACZPDN_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298998.1"
                     /translation="MKRNRLIAAVLTTSLILSGCAASEKDKDKKVDEKVSQKERDKSK
                     SIKKEKLKEVKETTEKASQEQETQASQVEETPVITGISSLYTGEELLAYANDHLLDFW
                     RTYYCFMAGTYFEGSGEIGNKLITDPNIRSLQDVENVWYQKFSRRYPAPYLDMNINPY
                     KELPFWEENGQVYERYRIDGFVGVSFFFDHITQKTDDEVWFAYYSKGADGTINDTQEQ
                     WSFVYEDGHLKYGTITRNY"
     gene            complement(3742..3900)
                     /locus_tag="ACZPDN_10040"
     CDS             complement(3742..3900)
                     /locus_tag="ACZPDN_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1298999.1"
                     /translation="MEKTNTITKNEFKKSFVKHYRVGEKVVCTNNMIAEIIEYRNYKD
                     IDVRYEDG"
     gene            complement(3908..>4214)
                     /locus_tag="ACZPDN_10045"
     CDS             complement(3908..>4214)
                     /locus_tag="ACZPDN_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1299000.1"
                     /translation="CEEKYGNCSGKLQEMEGPALSEKLLKQLEFDDDISKRVQYLIAH
                     HHTYDHVDGIDYQILIEADCLVNLYEDGVSKEAVKKAYNRIFKTDCGKTICREMFGI"
BASE COUNT         1406 a          752 c          674 g         1382 t
ORIGIN      
        1 tgcgattatt ggtatagatg gctctcaaat gatgactgct cctgcaaatt atgatggtga
       61 tgatctagaa ttaaaaatgt atgagctatc agaaacatta gaatataatg cagaatttta
      121 ttcgtggact tataatatga accaatctca agaacctgta aatactgata ctgaatcaat
      181 taaaatgtct ccgcaatata gatttattta ttataaagcc actgttcaac ataaaactat
      241 agttgtagaa tatgtattga gtaaagttct taaagagtac aaagaaagta aataagaaag
      301 aagatgtcgt tattatgaaa aagtactttg cactactatt atcgtttgtt ttagcattta
      361 gcttaacagc ctgttcagat gctgaaactg agaaagctaa atcttcattt gaagaaacag
      421 cttcaatcgt tgagaagaac aacgagacta tcaacaagga tgttaaagca ttgcaacagt
      481 tgacaaaatc caaggttaaa cctttggatg atacagtttt aaaaacagca agagaaacta
      541 tcagccaagc aaaacaacaa atcgttgaag ttcctgaatg cccttctaat aaagaagata
      601 ttaaagaagc taacaaaaag ctaaaaaaga aagctgataa agctgagatt atccaagctt
      661 taacagactc aaaaaaagct atgaaagata gtattgctca acgtaaacaa gttaccaacc
      721 catctgaatc ctttgttttg gaaagattga acggaattcc aaacgtatcg caagcattgg
      781 ctgtgaatga agaaaacgat gtaaatggta tgcttcataa agcgggtgga tatacttctg
      841 ctattttctt cacatcggat ttagtagaca ctgctgcaaa ctatattgaa gatggtgatt
      901 ccattgaaaa aggtacagac ggtggtggat gtatcgaagt ctttgaaact gaagaagatg
      961 ctcaaaaacg tgatacatac ctgtctactt tcgatggtgg tggaatgtta tgcagtggat
     1021 cacataaagt tgttggtact gtaatcattc gtacatctaa ctatctaaca gcaacgcaac
     1081 aaaatgatat tacaacaaat atcatgaaca gtttaattgc tttaaagtaa ttaaatacta
     1141 caggaccgaa tcatcaatca atgatttcag tcttttttta tacccttctc ttttctcgaa
     1201 tcctctcaca acgctctcga aaaccgacaa cgacgaattg cacactttga aaataatgtt
     1261 ttcataggat tagttataaa agtaacattc gttctagaga gcgttcttaa agcaatcgta
     1321 aatcactcct ttaattgaga taacatcaaa aatcaagtat actagataca taattatatg
     1381 gagtgattgt gattaattta attaacgtta tatttgtaat cttaataggc atcgggttcc
     1441 ccatgacatt ctctaaaaag aggcggttaa gagtgtgttc tcttgggcta gtaactaaag
     1501 gtaaattttt ataaagaaaa caggtcaatt gtgataatat aggagaagaa aatatatgca
     1561 cagatattac cagaaaaatc taaaaaaatg gcataaaact ttaaattctt acatgaatta
     1621 tatcagctat gaactagagt cgcttactgg gcagtcttat gataccctca ttcaagaaat
     1681 ttggacaaac tatcaggagg agatgttgga aagattccct tacattggcg gagatgctgc
     1741 tagcggaaca aagaacctta tcggagctta catgtttgtg agcatgggtg aagtattaaa
     1801 aaaatatggt gttacgacag cgcagagcgg tcatttgatg attttgattt atgagcgtca
     1861 gtttaaaaag attccgtctt ttttacggag tataatgggt aaaatcttta ctaatccccg
     1921 atttcttaca aaaatattcc taaaaaagga taggaagaat gcattaaacg ctgcacagaa
     1981 tcccggaagt ttcgagacac agacacagat tccaccagaa aagggggtat gatttcagct
     2041 atcacaacct tgtttgtccg ctagcggatt ttgcaaaaaa atacggatat gaagaataca
     2101 tgccttacct ctgtaatctg gattatgtca tgttcggtat tctgggtgcc ccactgtaca
     2161 gagagcatac ctgctttgat gatgattatt gtgatttcaa gttgaaactt tctgccaaac
     2221 cgatggacta tgatccaccg gtgttcacgc aggcgaatgg ttataaatag ctaactccca
     2281 cattttaatt gaattctatc tagaacagga taacgaattg acagatgcag aagtggaaga
     2341 aacccatcgt acgctgctta tgaatgattc agattttaaa tagaaaccaa aggactgatt
     2401 aataagtcag cccttttatt atgctcgctc atcatgagaa accttctcat ccccatccaa
     2461 gcaaaacaaa agaaggcttt tttaagcctt ctagtaattt ctggtaatcg ttccgtattt
     2521 taaatgcccg tcctcgtata cgaatgacca ttgctcttgt gtatcattga ttgttccatc
     2581 agcgcctttt gagtaatatg caaaccacac ttcatcatct gttttttgtg taatatgatc
     2641 aaaaaagaat gaaactccga caaatccatc aatacgatat ctttcataaa cttgtccatt
     2701 ttcttcccag aatgggagtt ctttatatgg attgatattc atgtctaaat aaggagcagg
     2761 atatcttctt gaaaacttct gataccatac attctcaaca tcctgtaacg agcgaatatt
     2821 tggatcagta atcaatttat ttccaatttc acctgagcct tcaaaatatg tacccgccat
     2881 aaaacaatag taagtacgcc agaaatcaag aagatgatca tttgcgtaag ctagaagttc
     2941 ttcgcctgta tatagacttg aaatacccgt aataactggt gtttcttcaa cctgactagc
     3001 ttgtgtttcc tgttcttgcg atgctttttc cgtagtttcc ttaacctctt ttaatttctc
     3061 tttctttatt gatttggatt tgtctcgctc cttttgagag accttctcat caaccttctt
     3121 atctttatcc ttttcagaag cagcacatcc agacaatatc aaagaagttg tcaagacagc
     3181 tgcaatcaac ctatttcttt tcatattctc accatttccc tgcttctagt atatcattca
     3241 gaataaaaac aaataaaatc ctgataaaat atctatataa gtgtgattta tggattactg
     3301 tatatatatg aacatgaaat tgagtgataa ctatcgatga agggcttctc tttttctgaa
     3361 tcggacttcc ggcgatgata gcaacaattg cttctgccaa ttattatcct ctaagctata
     3421 tcattttgat taaaacgtta gattgtttta ttgttcaata ataagcaatt ttcctcttcg
     3481 tatttgatga tgaagcattg tatatttata ataaaatgtc gcaaagcttt tgtgatatac
     3541 agtttcacca ttatcatcta tataatcaac atcgaaataa tttcttacgt taagtaacgt
     3601 tctgtatcca tgtaaagtac gtcttttctt tccaatattc ttttttacaa cattgaaacc
     3661 tctacaccat atcaccggtt tcttatctgg tctaggacaa ttattttgaa aaaaactttg
     3721 atatgttgta tgttcgataa tttacccatc ttcatatctg acgtcaatat ctttatagtt
     3781 tctatattcg ataatctctg caatcatatt atttgtacaa acaacttttt ctccaactct
     3841 ataatgctta acaaaacttt ttttaaattc attctttgtt attgtatttg tcttttccat
     3901 atttcaccta tattccaaac atttctctac aaatggtttt accgcaatct gttttaaata
     3961 tacgattata agcttttttt acagcttctt ttgatacacc atcctcatag agattaacca
     4021 aacagtctgc ttcaatcaat atttgataat caataccatc tacatgatca taggtatggt
     4081 gatgtgcaat taaatactga actcgtttgg atatatcatc atcaaattcc aattgtttca
     4141 acaatttttc agataatgca ggaccttcca tttcctgtaa tttaccgcta caatttccat
     4201 atttttcttc acac
//
LOCUS       DBMDWL010000175         4171 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_9108_length_4171_cov_2.203686, whole genome shotgun sequence.
ACCESSION   DBMDWL010000175 DBMDWL010000000
VERSION     DBMDWL010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9108_length_4171_cov_2.203686"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="ACZPDN_10050"
     CDS             <1..800
                     /locus_tag="ACZPDN_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1299001.1"
                     /translation="LLDTFTPIELLFMRFLIGFLTLCILSPKILKLKNRKDEIWYILS
                     GLTGVFLYYFIESTALVYTYATNVGVIISIAPFFSSILAYLCFKDEPFKMNFVLGFIV
                     AFIGIFFISFNGQQMHLSPKGDILTIVAAIMWAIYSVVLKKVNALGHTSIQNTKRIFA
                     WGLFFMTLSLPILGFDVSIDEILIPRNLLNMLFLGVIACAGCFVIWNHSCSILGSVRT
                     NQYVYLNPIATIIFSVIILHEKITLCIVLGTILTLSGLIISEYKKES"
     gene            complement(797..1075)
                     /locus_tag="ACZPDN_10055"
     CDS             complement(797..1075)
                     /locus_tag="ACZPDN_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299002.1"
                     /translation="MYSDILKCSIQNEDANFKGKTKPFTHTILGQTPFMAGAIDPMMY
                     VGIKLTLKDGTVLPIYVSKKKALLNSDMYLSDLKETKSILLKIQERIG"
     gene            complement(1690..>2169)
                     /locus_tag="ACZPDN_10060"
     CDS             complement(1690..>2169)
                     /locus_tag="ACZPDN_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1299003.1"
                     /translation="GLSILFNRNASSGGLDIVAKIMNKYLHMELGKAMSLSGMCVALS
                     AALVYDKKTVVLSILGTYFNGIVLDHFIFDHNIKRRVCIITPKEKELREFIIHELHSG
                     ATVYEAYGAYNMEKRNEIITIVDKAEYQKLMKFINTLDPKAFITVYNVSDMRYQPKI"
     assembly_gap    2170..2179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2180..2515)
                     /locus_tag="ACZPDN_10065"
     CDS             complement(<2180..2515)
                     /locus_tag="ACZPDN_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1299004.1"
                     /translation="MNKQYNYKNILKEAMILTLAVGIIAAAVYFFLVPSHTSVSSISG
                     LGIILSNFVPLPLSAITMILNVVLLIIGFLTCGKEFGVKTVYTSIMLPIFLAFFEILF
                     PNFVSFTNSQ"
     gene            complement(2653..3687)
                     /locus_tag="ACZPDN_10070"
     CDS             complement(2653..3687)
                     /locus_tag="ACZPDN_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIR1299005.1"
                     /translation="MLTYTYLEKGKFELIEKPKPVLLHERDAIVKVTLASICSSDLHI
                     KHGSVPRAVPGITVGHEMVGIVEQVGNKVTHVKPGDRVTVNVETFCGECFFCQNGYVN
                     NCTDDNGGWALGCRIDGGQAEYVRVPFADQGLNKIPDGVSDRQALFVGDVLATGYWAT
                     RISEIKEEDTVLIIGAGPTGICTLLCVMLQKPKRIIMCEKDESRLAFIHEHYPEVLTV
                     TPEKCKDFVLENSDHGGADVVLEVAGVPSTFQLAWQCARANAIVTIVALYDAPQVLPL
                     PDMYGKNLTFKTGGVDGCDCEKTLKLIAQGKINTEPLITHTYPLSRIAQGYELFENKR
                     DGVIKVAIEC"
     gene            complement(3962..4129)
                     /locus_tag="ACZPDN_10075"
     CDS             complement(3962..4129)
                     /locus_tag="ACZPDN_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299006.1"
                     /translation="MHVCVKESRIRRIAKPIISKLSERYTIMKFVLNELPLKIVKEDL
                     VQQRMHGNYDA"
BASE COUNT         1337 a          809 c          566 g         1449 t
ORIGIN      
        1 tactacttga tacatttaca cccattgaac ttctttttat gcgcttttta attggttttt
       61 taacactatg catcttaagt cctaaaatat taaaactaaa aaatagaaaa gatgagattt
      121 ggtacatcct tagcggatta accggtgttt tcttatatta ttttattgaa agtacagctc
      181 tagtctatac atatgccaca aatgtaggtg tcattatatc catagccccc ttctttagtt
      241 ccatacttgc ctatctatgt tttaaggatg aaccctttaa aatgaacttt gtgctaggct
      301 tcattgtagc ttttattgga atcttcttta ttagtttcaa cggtcaacaa atgcacctat
      361 ctccaaaggg agatattctt acaattgtag ccgccataat gtgggctata tatagtgttg
      421 tattaaagaa agtcaatgca ttaggacata cttccattca aaatacgaaa cgaatctttg
      481 cgtggggact tttctttatg acactcagcc ttccaatatt aggatttgat gtatccatag
      541 atgagattct aatcccacgc aatttattga acatgctttt cttaggtgtg atagcttgtg
      601 caggatgctt tgtgatttgg aaccattctt gttccatatt aggaagtgtt agaaccaatc
      661 aatatgtata tttaaacccc atcgccacaa ttatcttttc agtaattatc ctacatgaaa
      721 agattacgct atgtattgtt cttggaacta tacttacttt atctggctta attatatccg
      781 aatacaagaa ggaatcttaa ccaattcttt cttgtatttt taataaaatt gatttagttt
      841 ctttcaaatc acttaagtac atatcactat tcaacaacgc cttctttttc gatacatata
      901 ttggtaaaac tgtaccatcc tttaaagtca attttattcc cacatacatc ataggatcta
      961 ttgcaccagc cataaatggg gtttggccta gtattgtatg tgtaaatggt tttgtcttcc
     1021 ctttaaaatt ggcatcttca ttttgaatag aacattttaa tatatctgaa tacaagcatc
     1081 ttttaccatc aacttctaaa tattcatcct taaatttaaa acatatcttt tatcacttct
     1141 tttatctttt ctgcatccat acgatcttta atatattgat tcgtattgac catcgtcttt
     1201 tctttagata catatagggc caaaaaagtt tcatctttta atacaacctt tactccaata
     1261 aatagatatg gattttgttg aatccaagat ggtaatggtc cgctaggaag tatagccgta
     1321 aatggaacac cctttccttt ttactaagct ttttcattta agacacaaaa acgaactaca
     1381 tcactataag aatatgtatc ggatggaatg gtaaactgtt tattcaaatc atcaaatctt
     1441 atatatttat ctatattcag aattaaacct ccacattatt aagcagccaa taatcaggac
     1501 aatgccactc acaatgatac ttgtattttt agataagtaa taataagcca agcgattaca
     1561 caatttccaa ataccctttt atccataccc tatctcctta tcttcatatt aatcatttag
     1621 ccatttagaa tcaaatgtat tgtccaaata ggtcatttaa aaggaaaaaa aagtaaggtc
     1681 ttgccttact taaatctttg gttgatatcg catatccgat acattatata cagtgataaa
     1741 tgcttttgga tcaagtgtat taatgaattt catcaacttt tgatattccg ctttatctac
     1801 gatggtgatg atttcattac gcttttccat attgtatgca ccatatgctt catataccgt
     1861 tgctccacta tgtagttcat ggatgataaa ttcacgaagc tctttttcct ttggcgtgat
     1921 tatacatact cttcgtttaa tattatgatc aaaaataaaa tgatcgagaa cgattccatt
     1981 aaaataagtg cccaaaatac ttaacacaac cgttttctta tcataaacaa gagctgcaga
     2041 taaggcaaca cacatacccg ataatgacat tgcttttcct agttccatat gcagatattt
     2101 attcataatc tttgcgacaa tatcaagacc gcctgatgat gcatttcgat taaataaaat
     2161 actcaatccn nnnnnnnnnt tgactgtttg tgaaagatac aaaatttgga aacaggattt
     2221 caaaaaatgc aagaaaaata ggtagcataa tacttgtata gactgttttt acaccaaatt
     2281 ctttgccaca agttaaaaag ccaataatta ataaaacaac atttaagatc atggtaatgg
     2341 cagataatgg taatggtaca aaattagata gtatgatacc taatccagaa atactactta
     2401 cagaagtatg actaggcact agaaaaaaat agacggctgc tgcaataatt ccaactgcga
     2461 gtgtcaaaat catggcttcc tttaagatat ttttataatt atattgttta ttcatgaaaa
     2521 ctgtctccta ataattcgct attactatta ttttatcaaa aagtaatagt tcattccatc
     2581 ctattgtgaa tacggatctt tacccaaatc aaaaggaatc ctaaaacaca tgattatgga
     2641 ttccttttta aattaacact caatggctac tttaattaca ccatcacgtt tgttttcaaa
     2701 tagttcatac ccttgcgcaa ttcgactaag aggatatgta tgtgtaatca aaggttctgt
     2761 attgatctta ccttgtgcaa tcaattttaa agtcttttca caatcacaac catcaacacc
     2821 acctgttttg aaagtcagat ttttcccata catgtcggga agtggtaaaa cttgtggtgc
     2881 gtcatataaa gctacgattg tcacaattgc atttgctctt gcacattgcc atgcaagctg
     2941 aaaagtagat ggaacacccg caacttcaag aacaacatct gcaccaccat gatcactatt
     3001 ttctagcaca aagtctttgc atttttctgg agttactgtc aaaacttctg gataatgttc
     3061 atggataaaa gcaagacgag attcatcttt ttcacacata attattcttt ttggcttttg
     3121 aagcataaca catagtaaag tacaaatacc tgtaggacca gcaccaataa tcaatacagt
     3181 atcctcttcc ttgatttctg aaatacgagt tgcccaatat cctgttgcta atacatcccc
     3241 tacaaacaaa gcttgtcgat cactgacacc atcaggtatt ttattcaatc cttgatctgc
     3301 aaatggaaca cgcacatatt ctgcctgtcc gccatcaatt cgacatccaa gagcccatcc
     3361 tccattatca tccgtacagt tgtttacata gccgttttga cagaaaaaac attctccaca
     3421 aaatgtttct acattcactg ttacacgatc tccaggtttt acatgagtta ctttatttcc
     3481 aacttgttct acaataccaa ccatttcatg accaacagta ataccaggca cggcacgagg
     3541 aacactacca tgtttaatat gcagatcact tgaacaaata ctcgcaagtg ttacttttac
     3601 aatcgcatcc ctttcatgaa gaaggactgg ttttggtttt tctatcaatt caaattttcc
     3661 tttttctaaa tacgtatatg tcaacatatt tttcgactcc ttttctattc gtacttatta
     3721 aacgatttac caatttttta ctaaatgcaa tcctttttta tagcctgctg cactttcttt
     3781 tctacttcat cttccaaaac attatccatg ttctgtgcag atatcacatc cagaggacca
     3841 atccccctat ggaacataat tcaaaaaaat tcttaagtgc agccggatag gacatattcc
     3901 aaaacgaaga ggctataacg attcaatctg catcacgaat tgtcattgcc tatgatagtg
     3961 attatgcatc atagtttcca tgcattcttt gttgaacaag atcttccttt acgattttaa
     4021 gaggaagttc atttaaaaca aacttcataa tagtatatct ttcagatagc ttagaaataa
     4081 ttggcttggc aattctgcgg attctagact cttttacgca tacatgcatc tatatacact
     4141 agtgatttca tatttttcct catttctttt t
//
LOCUS       DBMDWL010000176         4098 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_9353_length_4098_cov_2.853390, whole genome shotgun sequence.
ACCESSION   DBMDWL010000176 DBMDWL010000000
VERSION     DBMDWL010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9353_length_4098_cov_2.853390"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(30..458)
                     /locus_tag="ACZPDN_10080"
     CDS             complement(30..458)
                     /locus_tag="ACZPDN_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013577.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1299007.1"
                     /translation="MTLDKKITLYENLTPVEVRIGNYIMNHKEVILNSTIQDLEKEVF
                     VSKPAIHRFCKKIGLNGFNDLKVELAKEEKMEEKNWVDANVPFYEKDQSKEIANKLMD
                     LYKSTIQGTYKCLDAKCMDEIAKFVNENGLYNAFKHLELI"
     gene            551..2698
                     /locus_tag="ACZPDN_10085"
     CDS             551..2698
                     /locus_tag="ACZPDN_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIR1299008.1"
                     /translation="MDENIISLIAKELNISISQVKNTLELLEEGATVPFIARYRKERT
                     KGLDEEQIRVIQENYAYQVNLAKRKEEVLARIETLGKLDDEIIKNVNACTKLSQVEDI
                     YRPYKQKKKTRASVAIANGLQPLADTFMSFPRYFKETELDAYINENVKDRDAAIQGAC
                     DIIAEKVSDDVDVRNKILDSMTNFGRIVTSEKKDHEDDHKVYKMYYDYSERVNTLAPH
                     RIMAIDRGEKEKVLNVSISFNEEYIENWVCRRFIRFNNSGTSEYVRTAILDGLKRLAY
                     PSIERMVRSALSEKAHESSIDVFSMNLEKLLLQPPMKDKVILGFDPAFRTGCKLAVID
                     ASGKKLTVDVIYPHQPNAKVKESEQKLVQLCNEYHVNLIAIGNGTASRESEAFVANTI
                     KKFNLPVSYTIVSEAGASVYSASKLAIEEFPDLHVEQRSAISIARRLMDPLSELIKID
                     PQSIGVGQYQHDLPTARLKERLDFVVEKAVNRVGVNINTASVSLLKNVAGLNNASATS
                     IVSYREENGKIESRTQIKKIPKIGPKAFEQAAGFLRIEDGKEPLDRTSIHPESYKATK
                     VLLKELGLDTLDLGTQKAKDVISNCDTKQLMQDTGLDSYTLKDILDAICMPLRDYRDK
                     YDAPLLRKDVLEIEDLHINDKLEGTVRNVVDFGAFVDIGLHEDGLVHVSKMSTKRVKH
                     PSDVVSVGDIVTVWVYNIDQEKQKVQLTMVNPN"
     gene            2780..3682
                     /locus_tag="ACZPDN_10090"
     CDS             2780..3682
                     /locus_tag="ACZPDN_10090"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1299009.1"
                     /translation="MDINTIVTQMTILLLIMIVGFVGAKKNIFNDEVNVYASNILLKI
                     GIPGLIISSVKNGSPFTGNELFNVLGIFSIFVIFTGIVSKIIVEVLHIQENKALWQFM
                     ILFTNAGFMGLPAIQAILGDQAMLYGALFLLPLNCLMYGYGESLFRKGGFSLKKFMNG
                     PMIASVIALVLCIFNVKLPYILAQTCTYVGNVTTPLSMMVIGAGLVKMDVKELLDLKL
                     WAFTIFKMILLPLFYMFSLSLFHIDPLIENTLILIMCMPVASNSSVYALMYGEDATLA
                     SKAIFLTSVACVVTIPIVFILHTL"
     gene            3777..4082
                     /locus_tag="ACZPDN_10095"
     CDS             3777..4082
                     /locus_tag="ACZPDN_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299010.1"
                     /translation="MEKWTQEQYAARLQEMKQEAHDKMWLYIEVNAKEFMNECEPGAK
                     NLATCCKAMLDAMLEGDGFIVEPKIRTKVAGTLTVRYYVDNLHPGRRKYADVMKEQQ"
BASE COUNT         1394 a          569 c          792 g         1343 t
ORIGIN      
        1 agaaaaaaga agtgaaattg attcacttct tatataagtt ctaaatgttt aaaggcattg
       61 tacaaaccat tttcatttac gaatttggca atctcatcca tacatttggc atctaaacac
      121 ttgtaagtac cttgaattgt acttttatac agatccatca atttattggc gatttcctta
      181 gattgatcct tttcgtaaaa aggcacattc gcatcaaccc agtttttctc ttccatcttt
      241 tcttctttgg caagttcaac ctttaaatca ttgaatccat tcagtccaat tttcttacaa
      301 aaacgatgaa ttgccggttt tgatacaaac acttcttttt ctaagtcttg tattgtactg
      361 tttaatatca cttctttatg attcattata taatttccaa tacgaacttc aaccggagtt
      421 aagttttcat ataaagtaat tttcttgtct aatgtcataa atacctctac taataatact
      481 ttaacacaca aaataggaat aaacgaatga attctttggt ataataatag agttgaaatg
      541 aggttttact atggatgaaa atataatttc cttaattgcg aaagaattga atatttcaat
      601 ttcgcaagtt aagaatacat tggaattatt agaagaagga gctacagttc cttttattgc
      661 acgttatcgt aaagagcgta caaaaggttt ggatgaagaa caaattcgtg tcattcaaga
      721 aaattatgcg tatcaagtaa atttagctaa gcgtaaggaa gaagttttgg cacgtattga
      781 aacattagga aaattggatg atgaaattat taagaatgtc aacgcatgta caaaactatc
      841 acaagtagaa gatatttatc gtccttacaa acaaaagaaa aagacgcgtg caagtgttgc
      901 gattgccaat ggtctacagc cattagccga tacattcatg tcgtttccac gttattttaa
      961 agaaacagaa ttggatgctt acatcaatga aaatgtaaaa gacagagacg ctgctattca
     1021 aggtgcttgt gatatcattg ccgaaaaagt gagtgatgat gtagatgtac gtaataaaat
     1081 attagattca atgacaaact ttggtcgtat tgttacaagt gaaaagaaag atcatgaaga
     1141 tgatcataaa gtatataaga tgtattatga ctattcggaa agagtcaata ctttagcacc
     1201 acatcgcatt atggctattg atcgcgggga aaaagaaaaa gtattaaatg tttcgatttc
     1261 ttttaatgaa gaatatattg aaaactgggt atgtcgtaga tttattcgtt ttaataacag
     1321 tggcacaagt gaatatgttc gtactgctat tttagatggt ttaaaaagat tggcatatcc
     1381 ttcaattgaa agaatggttc gttctgctct aagtgaaaag gcacatgaat caagtattga
     1441 cgtattttct atgaacttag agaagttgct acttcaacca cctatgaaag ataaagtaat
     1501 tctaggcttt gacccggcct ttagaaccgg atgtaaattg gctgttattg atgcatctgg
     1561 taaaaaatta acagtcgatg tgatctatcc acatcagcca aatgcgaaag taaaagagtc
     1621 tgaacaaaaa ttagttcaat tatgtaatga atatcatgtg aatttaattg cgattggcaa
     1681 tggtacggca agtcgagaaa gtgaagcttt cgttgcgaat acaatcaaga aatttaattt
     1741 accagtatcc tataccattg tttctgaagc cggtgctagt gtatatagtg catctaaatt
     1801 agctattgaa gaatttccag atttacatgt tgaacaaaga agcgccattt cgattgcgcg
     1861 aagattgatg gatccacttt ctgaattgat taaaatcgat cctcaatcga ttggtgttgg
     1921 tcaatatcaa catgatttac caaccgcaag attaaaagaa cgtttggatt tcgtagttga
     1981 aaaagccgtt aaccgcgtag gtgtaaatat caatacggca agtgtttcct tacttaaaaa
     2041 tgttgctgga ttgaataatg cgagtgctac aagcattgtg tcatatcgtg aagagaatgg
     2101 aaaaattgaa tcgcgtacac aaattaaaaa gattcctaag attggtccaa aagctttcga
     2161 acaagcagct ggattcttgc gtattgaaga tggaaaagaa ccgttggatc gtacaagcat
     2221 tcaccctgaa tcgtataagg caacgaaagt attgttgaaa gaattagggt tggatacttt
     2281 agatttagga acacaaaagg caaaagacgt gatttctaat tgtgatacaa aacaattgat
     2341 gcaagacact ggattagata gttatacatt gaaggatatc ttagatgcga tttgtatgcc
     2401 attaagagat tatcgtgata aatatgatgc gccattacta agaaaagatg tattggaaat
     2461 cgaagactta catattaatg ataagctaga aggtacagta cgtaatgtgg ttgactttgg
     2521 tgcctttgtc gatattggac tacatgaaga tggtttagtt cacgtttcaa agatgagtac
     2581 aaaacgagta aaacatccaa gtgatgtagt atctgtagga gatattgtta cggtatgggt
     2641 atacaatatt gatcaggaga aacaaaaggt acaactaaca atggtcaatc caaattgaca
     2701 atcaacaaac agagggttat tatttttaca catgtaggac aacgcattgt tgtcctttta
     2761 cttttagaag ggaattatta tggatattaa tacgattgtt acgcaaatga ctattttatt
     2821 gttgattatg attgttggtt ttgtgggagc taaaaagaac atctttaatg atgaagtaaa
     2881 tgtatatgca tcaaatattt tgttaaaaat aggtattcca ggcttgatta ttagttcagt
     2941 taaaaatgga tctccattta caggaaatga actatttaat gtacttggta ttttttcaat
     3001 atttgtgata tttacaggga ttgtatctaa aattattgtt gaggttttac acattcagga
     3061 aaataaagcc ctttggcaat ttatgatttt atttaccaat gccggtttta tgggattgcc
     3121 cgccattcaa gctatattag gggatcaagc tatgctttat ggagctttat ttttattacc
     3181 actaaactgt ttgatgtatg gatatggaga gagtttattt cgtaagggag gtttttcgct
     3241 taagaaattt atgaatggcc caatgattgc ttctgttatt gcgcttgtcc tttgtatttt
     3301 taatgtgaag cttccgtata tcttggcaca gacatgtaca tatgtcggta atgtgactac
     3361 acctttatct atgatggtaa ttggtgctgg tttagtcaaa atggatgtta aagagttatt
     3421 agatctgaag ttatgggcat ttacaatatt taaaatgatt ttattacctt tgttctatat
     3481 gttttctctt tctttatttc acattgatcc attgattgaa aatacgctta tactcattat
     3541 gtgtatgcca gtggcaagta attcaagtgt atatgcactg atgtatggtg aagatgcaac
     3601 attagctagt aaggccatat tcttaacaag tgtggcgtgt gtagtcacga ttccaattgt
     3661 gtttatttta catactttgt aaaaagcatg acaaatgcag ctataaataa aggcgaaagt
     3721 ttgagttatt gggtagaatg ttgtatgatt aggatataag aaaggacgtt gtgattatgg
     3781 aaaaatggac tcaagaacag tatgctgctc gtttacaaga aatgaaacaa gaagctcatg
     3841 ataaaatgtg gttatacatc gaagtgaatg ccaaagaatt tatgaatgaa tgtgaaccag
     3901 gagcaaagaa tttagctaca tgctgcaaag ctatgttaga tgcaatgtta gaaggagatg
     3961 gctttattgt agagcctaag attcgtacta aggttgctgg aactttaaca gttcgttact
     4021 atgttgataa cttacatcca ggtcgtagaa aatacgcaga tgtaatgaag gaacaacaat
     4081 aaaaaaaaga atcatttc
//
LOCUS       DBMDWL010000177         4075 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_9430_length_4075_cov_2.188978, whole genome shotgun sequence.
ACCESSION   DBMDWL010000177 DBMDWL010000000
VERSION     DBMDWL010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9430_length_4075_cov_2.188978"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="ACZPDN_10100"
     CDS             <1..816
                     /locus_tag="ACZPDN_10100"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1299011.1"
                     /translation="LMRVHENDDSRTKDAMAYSLMNGGKRVRPILLFAVLEGYGMDAH
                     VGYPCACAIEMIHTYSLVHDDLPAMDNDDLRRGKPSCHKAYDEATAILAGDGLLTKAF
                     EVVLDSECSAKQKVALVKALSWYAGIDGMIYGQTLDLQAENDTNPTFALLQEIDHYKT
                     SKLLTLPLVCGCILANKFDDIDHFRKIGLVLGLEFQMQDDILDVVGSEEVVGKSLSDE
                     DNHKVTAVSLLGLEGAKDMVKSYDETIRKSLDKVSCDVTSLTKVLDTLLKRTY"
     gene            905..>2693
                     /gene="dxs"
                     /locus_tag="ACZPDN_10105"
     CDS             905..>2693
                     /gene="dxs"
                     /locus_tag="ACZPDN_10105"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1299012.1"
                     /translation="MRKISQVYDIESPRQIKDLTIDELTQLCSDIRAFLIDSISKTGG
                     HLSSNLGIVEVTVAMHYVFDSPKDKMIFDVGHQCYTHKILTGRSTQFATLRKKGGLSG
                     YQKRSESKYDCWEAGHSSTSLSAALGYAIARDLKQEDYNVIALIGDGSLTGGMALEAL
                     NDIGSKQKKMVIIFNDNNMSISKNYSGVEKRITDIRASHLYIDLKHDVKNNLKSNKLG
                     SNVLNTLSHFRDRIKDEVIDAPLFKEFNLDYMGPVDGHDIQSLIKVFEAAKEHDGPIV
                     VHVLTQKGKGYKYAEQDKVGKWHGVSPFDVATGKSLSLLPPNEISWSQVISNTVMDLA
                     EKDKTIVAITPAMAQGSKLLEFQKRFPDRFFDCGIAEQHAVTMACAMALGGLKPFVSI
                     YSSFLQRAYDQVNHDMARMNVGVVIGIDRCGLVGDDGETHQGVFDIAMLRSVPNLILS
                     QPKDAKEAQNMIYTAFDSKKPFCIRYPRGNALYAKNKSYSLIPIGSWEKFVVGTPTQI
                     VITYGPDVDHVINKARENKMGLLVVNARFFKPIDEVMMKDLLKMDLSITVYETDVKIG
                     GLSSAVLEFINTLDEKIHIIGIEDHYVCHG"
     assembly_gap    2694..2703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2704..3436
                     /locus_tag="ACZPDN_10110"
     CDS             <2704..3436
                     /locus_tag="ACZPDN_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1299013.1"
                     /translation="MDKIRLDKALLKTISSRAKAQDMIKEGKISVNKKVITKASYLVS
                     DEDDIVVTHTQDEFVSRGAFKLKGCLDKYNVDISNQVVLDIGASAGGFTDVCLRYGAK
                     KVYAVDVGHLQLAKELDQDCRVVKMEGHNAREIVPDWFDESIDFMCMDVSFISCKTIL
                     KHVLHVLSISHIAILVKPQFECGPAALNKHGVLKNSKLALQIVEDVKAFLFQYYKSVE
                     AMPSIIEGRSGNQEYMVYAKDLRIS"
     gene            3542..>4075
                     /locus_tag="ACZPDN_10115"
     CDS             3542..>4075
                     /locus_tag="ACZPDN_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024867.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1299014.1"
                     /translation="MIEQLSVKDYVLFESCIIDFTTGMSVITGETGAGKSLLIDAIGY
                     LSGDRIKSNVIRNGKDKAILSMVLTSNEKVNSILDENGFEVDDQVIISRTILNNNKST
                     VRINQQITTLSFVRKIVNLLVDVHSQMDTYRLMDTKLQMELLDSYAKVEDLKSSVKEA
                     YFSYSNVLNELETLKNEE"
BASE COUNT         1360 a          519 c          845 g         1341 t
ORIGIN      
        1 ttgatgcgag tacatgaaaa tgatgatagt cgtacaaaag atgcgatggc ctattcattg
       61 atgaatggcg gaaaaagagt acgtcctata ttgttgtttg ctgtactaga aggctatgga
      121 atggatgctc atgttggata tccttgtgct tgtgccattg aaatgattca tacttattca
      181 ttagttcatg atgatcttcc agctatggat aatgatgatt tacgtcgcgg taaaccaagt
      241 tgtcataaag cttatgatga ggcaacggcc attcttgcag gggatggatt acttaccaaa
      301 gcttttgaag tcgtacttga tagtgaatgt agtgcgaaac aaaaagtggc attagttaaa
      361 gcactttctt ggtatgcagg tattgatggt atgatctatg gtcaaacatt agatttgcag
      421 gctgaaaatg atacgaatcc aacatttgcc cttttacaag aaatcgatca ctataaaaca
      481 agtaaattat taactttgcc tttagtatgt ggttgtatat tagcaaataa atttgacgat
      541 attgaccatt tccgtaagat tggacttgtg ttagggttgg aatttcaaat gcaagatgat
      601 attttagatg ttgttggtag tgaagaagtg gttggaaaat cattgagtga tgaagataat
      661 cataaagtaa cagctgtatc tttattaggt cttgaaggag ctaaagatat ggttaaaagt
      721 tatgatgaaa ccattcgtaa atctttagat aaagtcagtt gtgatgtaac ttcactaaca
      781 aaagtattgg atactttatt aaagcgtaca tattaatata tggaatttct ttgaaaaaag
      841 gggtatatta ataatagcta aaactagtct ttagcggact agttttttta acgagggtta
      901 taaaatgaga aagattagtc aagtgtatga tattgaatca ccacgtcaaa tcaaggattt
      961 gacaatcgat gagcttacac aattgtgtag tgatattcga gcttttttaa ttgattcaat
     1021 ctcaaaaaca ggtggtcatt tatcaagtaa cttaggcatt gttgaagtca cagtggctat
     1081 gcactatgtc tttgattcac ctaaagataa aatgattttt gatgttggac atcaatgtta
     1141 cacacataaa atattaactg gaagatccac acaatttgcg acattacgca aaaaaggtgg
     1201 tctttcaggt taccaaaagc gtagtgaaag taaatatgat tgttgggaag ctgggcattc
     1261 ttcaacctct ttatcggctg cactcggcta tgcgattgca cgcgatctaa aacaggaaga
     1321 ttataatgtc attgccctta ttggagatgg ttcattaaca ggtggaatgg cacttgaggc
     1381 attaaatgat attggttcta agcaaaaaaa gatggtcatt atatttaatg ataataatat
     1441 gtcaatttct aagaattata gcggagttga aaaacgtata acggatattc gtgcttcaca
     1501 tttatatatt gatttaaaac atgatgtaaa gaataactta aaatcgaata aattaggtag
     1561 taatgtatta aacacactgt ctcattttag agacagaatt aaagatgaag tgattgatgc
     1621 acctttattt aaagagttta atttagatta tatgggacct gtggatggtc atgatattca
     1681 aagtttaatt aaggtgtttg aagctgccaa agaacatgat ggtcctattg tagttcatgt
     1741 attaacacaa aagggtaaag gttataaata tgcggagcaa gataaagttg gaaaatggca
     1801 tggtgtaagt ccttttgatg tggctacagg taaatcctta agtcttcttc caccaaatga
     1861 aatttcttgg tcccaagtta tttctaatac ggtcatggat cttgccgaaa aagataaaac
     1921 gattgttgcg attacaccag ccatggcgca gggaagtaag ttattagaat ttcaaaaacg
     1981 ttttccagat cgtttctttg attgtggtat tgcagaacaa catgccgtaa caatggcttg
     2041 tgcaatggca ttaggtggtt taaaaccatt tgtcagtatt tattcttctt tccttcaaag
     2101 agcttatgat caagtcaatc atgatatggc tagaatgaac gttggcgttg tgattggaat
     2161 cgatcgttgt ggtttagttg gagacgatgg tgaaacccat caaggtgtat ttgatattgc
     2221 gatgcttaga agtgttccga atttgatttt gtcacaacca aaagatgcta aagaagctca
     2281 aaatatgatt tatacagctt ttgattcgaa aaaaccattc tgcattcgtt atcctagagg
     2341 aaatgcgttg tatgccaaaa ataaatcgta ttcgttaatt ccgattggtt cttgggaaaa
     2401 atttgtggtt ggaactccaa cacaaattgt gattacatat ggtccagacg ttgatcatgt
     2461 gatcaataag gcaagagaaa ataaaatggg tttattggtt gtcaatgcac gtttctttaa
     2521 gcctatagat gaagttatga tgaaagattt attaaagatg gatctttcga ttacagtata
     2581 tgaaacagat gttaaaattg gaggactttc aagtgctgta ttggaattta taaatacact
     2641 tgatgaaaag attcatataa taggtattga ggatcactat gtatgtcatg gttnnnnnnn
     2701 nnncatggat aagatacgat tagataaagc tttacttaaa acgatatcta gccgtgcaaa
     2761 agcacaggat atgattaaag aagggaaaat cagtgtcaat aaaaaagtaa tcacgaaagc
     2821 tagttattta gtgagtgatg aagatgatat tgtcgtaaca catacccagg atgaatttgt
     2881 gtcgcgtggt gcttttaaat taaagggttg tcttgacaaa tacaatgtcg atatttcaaa
     2941 tcaagttgta ctggatattg gagcaagtgc aggtggtttt acagatgtat gtcttcgtta
     3001 tggcgctaaa aaagtatatg cggttgatgt tggtcattta caactcgcaa aagaattaga
     3061 ccaagattgt cgtgttgtaa aaatggaagg gcataacgca agagaaattg tgcctgactg
     3121 gtttgatgaa tccatagatt ttatgtgtat ggatgtaagt tttatatctt gtaagaccat
     3181 tttaaaacat gttttgcatg tattatccat atctcatatt gcgattttgg taaagcctca
     3241 atttgaatgt gggcctgctg ctttgaataa gcatggtgtt ctaaaaaatt cgaaacttgc
     3301 cttacagatc gttgaagatg taaaagcgtt tttatttcaa tattataaaa gcgttgaagc
     3361 catgccaagt attatagaag gaagaagtgg caatcaagaa tatatggtat atgccaaaga
     3421 tttaagaata tcttaatgtg aagatattct tattttttta tttacagctt cacccgtgat
     3481 gataatcatt tattttcctt tgaatttaag ttaaaataat aagtagactt gaggtggttt
     3541 tgtgattgaa caattgtcag taaaagatta tgtattattc gaatcttgta tcatagattt
     3601 tacaactgga atgagtgtga ttacgggtga aacaggtgcc ggtaaatcgt tattgattga
     3661 tgcgatcgga tatttgagtg gggatcgcat taaatccaat gtgattcgta atggaaaaga
     3721 taaggccata ttatctatgg ttttaacttc gaatgaaaaa gtgaattcga ttttagatga
     3781 aaatggattt gaagtggatg atcaagtgat tatttcgcgt acaattttga ataataataa
     3841 aagtactgta cggatcaatc aacagatcac aactctttct tttgttcgta aaatcgtcaa
     3901 tttattagtg gatgttcatt cgcaaatgga tacatatcgt ttaatggata caaaacttca
     3961 aatggaatta ttggatagtt atgccaaggt agaggattta aaatcatctg taaaagaggc
     4021 ttatttttca tattcaaatg ttttaaatga attagaaaca ttaaaaaatg aagaa
//
LOCUS       DBMDWL010000178         4071 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_9445_length_4071_cov_1.507305, whole genome shotgun sequence.
ACCESSION   DBMDWL010000178 DBMDWL010000000
VERSION     DBMDWL010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9445_length_4071_cov_1.507305"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="ACZPDN_10120"
     CDS             <1..1301
                     /locus_tag="ACZPDN_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1299015.1"
                     /translation="AKNTVSVIAMNSSSMEDVKSLEGAKLGVLTNIGKESTEKSLKDI
                     KKNKVTYTKSDYDNVPSLIKALYDGEVDAVVLNEAYRSNVCDIEEYANFNNETKVIHK
                     TVYYTKENSSSLAVSDITAKPFNILISGNHSFGSLDENSRSDVDMLVTINPVTSTILL
                     TSIPRDSYVTEVCDDYACNYGVTDKLTHTGMYGVDTTKDTIENLLDIEVNYVYRVNFT
                     SMINIVDALGGVDVTVPEGMAVSKFYTNSNLEGVHEGENHLDGKRALAYSRERKAYLD
                     GDVQRARNQQQVLQAMFKKATSPEIIKNYVNLLNALIGAFDTNMTTDEITSFIKYQIQ
                     AKPNWKFEQFVLKGDNDLRVSPELGSEVSVVILYDSYISIAHDKIQAVLDGKSSDTVE
                     AQEDTPAGTLSEEEIEAQIQYGLMTEAPIEEEGSEIYYGN"
     gene            1388..2263
                     /locus_tag="ACZPDN_10125"
     CDS             1388..2263
                     /locus_tag="ACZPDN_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR1299016.1"
                     /translation="MKVLFKFVLLSAMLISFTGCQKSNEVAIYQLSNQSYSQMMSYII
                     EDEGTTIVVDGGTAEDEDNLIKTIKKVSKDRKVEAWFITHYHKDHTGALAKYLQSGSN
                     EIKIDKVYYNFPEESWVERNEPHRYEDVVAIDQGLKTFDNKEVVSFNQKITVGEDQID
                     VLRTYNPEITENAGNNSSSVYKMNVQGTKILFLGDLGVEGGEELLSLNKEAIKNMDYV
                     QMAHHGQAGVNEDVYQVIDPKYCLWPTTNWLWKNEGKAYKTDETKKWVKALHVKKNYV
                     ADNGTVRIELKKKEK"
     gene            2280..3080
                     /locus_tag="ACZPDN_10130"
     CDS             2280..3080
                     /locus_tag="ACZPDN_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1299017.1"
                     /translation="MDFLRSLKKNKELIWFLAKDDIKKRYAGSSLGIVWAFAQPLVTI
                     TIYWFVFQIGLRSVAPGQYGDMPFLVWIMCGLIPWFFFSDGINSVTSVFLEYSYLVKK
                     VVFDINILPVIKIISAMFVHIFFIFLIFIVMALFGYMPSICYIQAFYYSFCMICLILA
                     IGYFTSSLSVFFRDLPQLVQVCLSAGMWLTPIMWAFDMLQGYWFSFIFKLNPMFYVVE
                     GYRSAILGHQWLFSNWKLTIYFWCVVVLLTLIGRNVFKKMKPHFADVL"
     gene            3092..>4071
                     /locus_tag="ACZPDN_10135"
     CDS             3092..>4071
                     /locus_tag="ACZPDN_10135"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1299018.1"
                     /translation="MDSVIEIKNISKIYNLYNKPSDRLKEALFARQSRHTEFAALNDV
                     SFNVNKGEILGIIGKNGSGKSTILKIITSVLTPTSGECIVKGKIAALLELGAGFNMEY
                     TGIENIYLNGQMIGFSKDEMDKKLQDIIDFADIGEHIYQPVKTYSSGMFARLAFSVAI
                     SVDPDILIVDEALSVGDVFFQNKCYRRFEEFRERGKTILFVTHDMGSVIRYCNRCVLL
                     NAGKKVAEGKPQEMVDLYKRIMVGQWNENEESSEESSPVQSSNVKNDQLWKDQISTNP
                     DIEVYGDGRADIIDFGIFSDTGDIGNNVYKGDYYSVKMKVRINEDNLNPI"
BASE COUNT         1432 a          482 c          819 g         1338 t
ORIGIN      
        1 aagcgaaaaa cactgtttca gtcattgcga tgaattctag cagcatggaa gatgtaaaga
       61 gtttagaagg ggctaagctt ggtgttttga caaacatcgg taaggaatcg actgaaaagt
      121 cattgaagga tattaaaaag aataaggtaa cttatacaaa atcagattat gataatgtgc
      181 ctagtttgat taaggctttg tatgatggtg aagtggatgc ggttgtatta aatgaagcgt
      241 atcgttcaaa tgtatgtgat attgaagaat atgccaattt caataatgaa acaaaagtga
      301 ttcataaaac ggtttattat acaaaagaaa acagttcttc attagcggtt tcggatatta
      361 ctgcaaaacc atttaatatt ttaattagtg gaaaccattc atttggctca ttagatgaaa
      421 attcaagatc cgatgtagat atgttagtta caatcaatcc agtgacttca actattttat
      481 taacaagtat tcctcgtgat tcttatgtta cagaagtatg tgatgactat gcatgtaact
      541 atggagtaac ggataagtta acacatacag gaatgtatgg tgtagatacg acaaaagata
      601 caattgaaaa tttattagat attgaagtca actatgtata tcgtgtgaac tttacaagca
      661 tgatcaacat tgtggatgct ctaggtggag tggatgtaac tgtaccagag ggtatggccg
      721 tttctaaatt ttatacaaat agtaatttag aaggtgtaca tgaaggtgaa aaccacttag
      781 atggaaaacg tgcattagct tattctcgtg aaagaaaagc gtacttagat ggggatgtac
      841 aacgtgctcg taaccagcaa caagtattgc aggctatgtt taaaaaggct acatcaccag
      901 agattattaa gaattatgtg aacttattaa atgccttgat tggtgctttt gatacaaata
      961 tgacaacaga tgaaattact tcttttatca aataccaaat tcaggcaaaa ccaaattgga
     1021 aatttgaaca atttgtgctt aaaggagata atgatctacg tgtaagtcca gaactcggtt
     1081 cggaagtcag tgtcgttatc ttatatgatt cttatatcag tattgcacat gataagattc
     1141 aagctgtact ggatgggaaa tcaagtgata cagtagaagc acaagaggat actccagcag
     1201 gaactttatc tgaagaagaa attgaagctc aaattcagta tggtttaatg acagaagctc
     1261 caattgaaga agaaggttca gaaatttatt acgggaacta acaagattct ttatttagaa
     1321 tgatgattga aaaagattag gggaagcttg gcttcccctg taatgttatg aaagactagg
     1381 tgtatttatg aaagtgttat tcaaatttgt tttattatca gctatgttga ttagctttac
     1441 tggttgccaa aaaagtaatg aagttgcgat ttatcaatta tcaaatcaat cctactcaca
     1501 aatgatgtca tacattattg aagatgaagg aacaacaatt gtggtagatg gtggtacagc
     1561 agaagatgaa gataatttaa taaagacaat caaaaaggtc agtaaagatc gtaaggtaga
     1621 agcttggttt ataacacatt atcataaaga tcacacaggt gctttagcaa aatatcttca
     1681 gagtggttca aatgaaatta aaattgataa agtgtattat aattttccag aagaatcttg
     1741 ggtagaaaga aatgaacctc atcgttatga ggatgtggtt gcgattgatc aaggcctaaa
     1801 gacatttgat aataaggaag ttgtttcatt caatcaaaaa attacagtgg gtgaagatca
     1861 aatagatgta ttaagaacat ataatccaga aatcactgaa aatgccggaa ataattcatc
     1921 gagcgtatat aaaatgaatg tacaaggaac gaaaatttta tttttaggag atttaggtgt
     1981 tgaaggtggc gaagaacttt tgagtttaaa caaagaagct atcaaaaata tggattatgt
     2041 tcaaatggct caccatggac aagcaggtgt aaatgaagat gtatatcaag tcattgatcc
     2101 taaatattgt ttatggccaa ccacaaattg gttatggaaa aatgaaggca aagcctacaa
     2161 aacagatgaa acaaaaaaat gggttaaagc tttacatgta aagaaaaatt atgtagcaga
     2221 caatggaact gtccgtattg aattaaagaa aaaagaaaaa tagattgcga ggaagaaata
     2281 tggatttttt gagaagtctt aaaaagaaca aagagttaat atggttttta gctaaggatg
     2341 atattaaaaa aagatatgcc ggatcctctt taggaatcgt atgggctttt gcacagcctt
     2401 tggtaactat tactatttat tggtttgtat tccagattgg attacgttca gttgcgccag
     2461 gacaatatgg agatatgcca tttttggtct ggatcatgtg tggtttgatt ccgtggttct
     2521 tttttagtga tggtataaac agtgtaactt cagtctttct tgaatatagt tatttagtaa
     2581 agaaagttgt atttgatatt aatattttac cggtcattaa aattatttca gccatgtttg
     2641 tacatatctt ctttatcttt ttgattttta ttgtcatggc tttatttgga tatatgcctt
     2701 cgatttgtta tattcaagcg ttttattatt cattttgcat gatttgtttg attttagcaa
     2761 ttggatattt cacatcaagt ttatccgttt ttttcagaga tttaccacaa cttgtacaag
     2821 tatgtttatc agcaggaatg tggttaacac ccattatgtg ggcttttgat atgcttcaag
     2881 gttattggtt ttcatttatt tttaaattaa atccaatgtt ttatgttgta gaaggatatc
     2941 gtagtgccat tttaggtcat caatggttat tttcaaattg gaagcttaca atttatttct
     3001 ggtgtgttgt tgtattgttg actttaattg ggcgaaatgt atttaaaaag atgaagcctc
     3061 atttcgcgga tgttttatag gagataagaa aatggatagt gttattgaaa taaaaaatat
     3121 ttccaaaatc tataatttat ataataaacc aagtgatcga ttaaaagagg ccttatttgc
     3181 acgtcaatct agacatacag aatttgcggc attaaatgat gtttctttta atgtaaataa
     3241 aggagaaatc ttaggaatca ttggtaagaa tggaagtgga aaatccacaa ttttaaaaat
     3301 tattactagt gtattaacac caacatcggg cgaatgtatt gtcaaaggta aaattgcagc
     3361 tttacttgaa cttggtgcag gtttcaatat ggaatatacg ggtattgaaa atatttattt
     3421 aaatggacag atgattggtt tttctaaaga tgaaatggat aaaaaattac aagacattat
     3481 tgattttgcc gatattggag aacatattta tcaaccggtt aaaacatatt caagtggtat
     3541 gtttgcacgt ttagcttttt cagtggctat tagtgttgat ccagatattt tgattgtaga
     3601 tgaagcttta tcggttggag atgttttttt tcaaaataaa tgttatcgtc gttttgaaga
     3661 atttagagaa cgaggaaaaa ctattttatt tgttacacat gatatgggta gtgtcattcg
     3721 ttattgtaat cgttgtgttt tattaaatgc aggaaagaaa gtggctgaag gaaagccgca
     3781 agaaatggtc gatctatata aacgaattat ggttggccaa tggaatgaaa atgaagagag
     3841 ttcagaagaa tcttcacctg ttcaaagttc aaatgtaaag aatgatcaat tatggaaaga
     3901 tcaaatcagt acaaatccgg atattgaagt ttatggagat ggaagagcgg atattatcga
     3961 ttttggaata ttctcagata caggagatat tggaaataat gtttataaag gagattatta
     4021 ttccgtaaaa atgaaggttc gtatcaatga agataacttg aatccaatct t
//
LOCUS       DBMDWL010000179         3988 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_9760_length_3988_cov_2.323900, whole genome shotgun sequence.
ACCESSION   DBMDWL010000179 DBMDWL010000000
VERSION     DBMDWL010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9760_length_3988_cov_2.323900"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="ACZPDN_10140"
     CDS             complement(<1..593)
                     /locus_tag="ACZPDN_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR1299019.1"
                     /translation="MKTQNIITVKPQGYCGGVLKAIEVAKKTRIQYPNQKITILGNLV
                     HNQYVKKALQSYSIDTIENKNKTRYELLDEINEGIVIFTAHGVSPKVYEKAKEKGLIL
                     VDASCPFVLQTQKIVKQYLDEDYTIFYIGKNKHPEAESIYTMSKNVYLIEPKKEIPNV
                     DTNKIFVTNQTTMSIYELKDTFDQIKQLYPSAIFHDE"
     gene            675..1997
                     /locus_tag="ACZPDN_10145"
     CDS             675..1997
                     /locus_tag="ACZPDN_10145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1299020.1"
                     /translation="MNNRIQEIMDLEHFKTLTPIQEQVLNRKNKNRDIIGVSSTGSGK
                     SHAFFMPIFEKLDFDQDCVQAVISAPTRELAYQLYDRCRKIAKHFNVRVKLVTGGMEK
                     VTQMEKQPQIVIGTPGRMKDMFIKDNVLRLDTAKIVVIDEADMTLEFGFLEDIDEILS
                     KMDKKVQMMVFSATIPESLQPFLKKYLYDPEFIEIKKEEAFQSDVKHILVPCKHKSYE
                     QKILDVLPCIQPYVCLIFANTTQEAQNIANTMRENGYDLVELHSGLESRQRMQAIKMI
                     QSQKKSYIVASDIASRGIDLEGITHVISCGFPKDLKFYIHRAGRTGRASRDGQCIALY
                     KESDTRSIQSLIKQGIEFEHQDVKNKQFVDLKPYVNKRPMKKDAFHEEVEKIVHKKKK
                     VKPNYKKRQRQEIDRMKRKKRREMIKSDIKRQQKERAKERQRQKGMDI"
     gene            1994..2833
                     /locus_tag="ACZPDN_10150"
     CDS             1994..2833
                     /locus_tag="ACZPDN_10150"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR1299021.1"
                     /translation="MIYLGSHVSFKSPNYFKGAIEEALSYKANACMIYTGPPSNTRRV
                     DVSKLKIEEAKKIMAEHDFSIDRVIVHAPYIINLANALKPETALFGQDFLKAELERVS
                     KIGAKTLVLHPGSHVGAGMDVGIEWIINGLNEVLDHDTSDVKIALETMAGKGNECGFT
                     FEQLQKIYLGIHKKSRIGFCMDTCHIWDAGYDITHFDEVLNQFDSILGLENLLCMHIN
                     DSKNELGAHKDRHENIGKGYIGFDVLYAIVHHPKLEHVTKILETPFVDGKACYKEEIS
                     ALR"
     gene            complement(2847..3896)
                     /gene="ispG"
                     /locus_tag="ACZPDN_10155"
                     /gene_synonym="gcpE"
     CDS             complement(2847..3896)
                     /gene="ispG"
                     /locus_tag="ACZPDN_10155"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1299022.1"
                     /translation="MNRTQTRQVQVKNLEIGHNNHVIIQSMCNIETKKADEVAKQILN
                     LEKMGCELIRVSCMDMEDAKAIKDIKKQIHIPLVADIHFDYRLALACIEAGADKIRLN
                     PGNIGSRENVEKVVKACKEKHIPIRIGINGGSLEKDIHEKYGKPTAQGMIESAKRHVK
                     ILEDLDFYDTVLSFKSSDPLLCIEAYRLAAKTFDYPLHLGVTEAGTTMTSAIKSSLAL
                     GTLLNEGIGNTIRISVNGAPEKEIPIVKELLKDCKLIKNVPNLIACPTCGRTQWDMEP
                     VVNEIESYLQTIHKEVTVAIMGCAVNGPGEAKHADIAIAGGKKEGLLIKKGKIIEKIP
                     QDQIVARLKEEIDKF"
BASE COUNT         1294 a          654 c          705 g         1335 t
ORIGIN      
        1 atttcatcat ggaagattgc ggatggatac agttgtttga tttgatcaaa agtgtctttt
       61 aattcataga tcgacattgt tgtttgattt gtcacaaaaa ttttgtttgt atcaacattt
      121 ggaatctctt tctttggctc aatcaaatat acatttttac tcattgtata aatcgattca
      181 gcttctggat gtttgttttt gccaatatag aaaatagtat agtcctcatc caaatattgt
      241 tttacgatct tttgtgtctg taagacaaat ggacaactcg catccacaag aatcaaccct
      301 ttttctttgg ctttttcata aacttttgga ctgacaccat gcgctgtaaa aatcacaata
      361 ccttcattaa tttcatccaa taattcgtaa cgtgttttat tcttgttttc aattgtatca
      421 atggaataag attgaagggc ctttttcaca tattgattat gtacaagatt tcctaaaatc
      481 gtaatctttt gatttggata ttgaattcgt gttttctttg cgacttcgat ggcttttaaa
      541 acgcctccac aatacccctg aggcttgact gtaatgatat tttgtgtttt catatataca
      601 ttatgcccta attatttgta tttgtaaaat ggaaagaatt ttgggataat actagagtaa
      661 aggatgtgaa atttatgaat aatcgtattc aagaaataat ggatttagaa cattttaaaa
      721 cattaacacc catccaagaa caggtgttaa atcgtaaaaa taaaaataga gatattatcg
      781 gtgtttcaag tacaggttca ggaaagtctc acgctttctt tatgccaatc tttgaaaaac
      841 tagattttga tcaagattgc gtacaagccg tcatttctgc gccaactcgt gaacttgctt
      901 atcaattata tgatcgttgt cgtaagattg ccaaacattt taatgtgcgc gtaaagcttg
      961 taacaggtgg tatggaaaaa gtaacgcaaa tggaaaaaca accacaaatc gttattggta
     1021 cacctggaag aatgaaagat atgtttatta aagataatgt cttacgttta gatacagcta
     1081 aaattgttgt cattgatgaa gcggatatga cattagaatt tggattctta gaagatattg
     1141 atgaaatctt atctaaaatg gataagaaag tacaaatgat ggtcttctcg gcaaccattc
     1201 cagaatcttt acaaccgttc ttaaagaaat atttgtatga tcctgaattt attgaaatta
     1261 aaaaggaaga agcttttcaa agtgatgtaa aacacatttt agttccttgt aagcacaaga
     1321 gttatgaaca aaaaatctta gatgtacttc catgtattca gccctatgtt tgtttgattt
     1381 ttgcgaatac gacgcaagaa gcacaaaata ttgccaatac gatgcgcgaa aatgggtatg
     1441 atctcgttga acttcattca ggtcttgaat cgcgtcaacg tatgcaggct attaaaatga
     1501 ttcaaagcca aaagaagagt tatatcgttg ccagtgatat tgccagtcgt ggaattgact
     1561 tagaaggaat tacacatgtg attagttgtg gcttccctaa agatttaaaa ttctatattc
     1621 accgtgcagg tcgtacggga agagcgagtc gtgatggtca atgtattgct ttatataaag
     1681 aaagcgatac tagatcgatt caatctttaa tcaaacaagg tattgaattt gaacatcaag
     1741 atgtgaagaa caaacaattt gtagatttaa aaccatatgt aaacaagcgt ccaatgaaaa
     1801 aggatgcgtt ccatgaagaa gttgaaaaaa tcgttcataa gaaaaagaaa gtaaaaccaa
     1861 attataaaaa aagacagcgt caagaaattg atcgcatgaa acgtaaaaaa cgtcgtgaaa
     1921 tgatcaaatc ggatattaag cgccaacaaa aagaacgtgc taaagaaaga caacgccaaa
     1981 aaggaatgga tatatgatct atttaggaag tcatgtttct tttaagagtc ctaactactt
     2041 taaaggagcc attgaagaag ctttatccta taaagcgaat gcctgtatga tctatacagg
     2101 accgcctagt aatacacgtc gagtggatgt atcgaaatta aagattgaag aagctaaaaa
     2161 aataatggct gaacatgatt tttcaatcga tcgtgttatt gtgcatgcac cctacattat
     2221 caatttggcc aatgctctaa aacctgaaac ggctttgttt gggcaagact ttttaaaggc
     2281 ggaattagaa agagtttcta aaattggtgc taaaacacta gtgctacatc caggaagcca
     2341 tgtaggtgct ggaatggatg ttggtattga atggatcatc aatggcttaa atgaagtctt
     2401 agaccatgat acaagtgatg tcaagattgc gcttgaaaca atggccggaa aaggcaatga
     2461 atgtggcttt acatttgaac aacttcaaaa aatttattta gggattcata aaaaaagccg
     2521 cattggtttt tgtatggaca catgccatat ttgggatgcg ggttatgata tcactcactt
     2581 tgatgaagtc ctcaatcaat ttgattcaat tttaggctta gaaaatctat tatgtatgca
     2641 tatcaatgat tctaaaaatg aattgggtgc ccataaagat cgtcatgaaa atataggtaa
     2701 aggctacatt ggctttgatg tattgtacgc tatcgtgcat catccaaagt tagaacatgt
     2761 cacaaaaata ttagagactc catttgtgga tggaaaagct tgttataaag aagaaatctc
     2821 agcactgcgc tgagattttt ttctaattaa aatttatcaa tttcttcttt taaacgagct
     2881 acaatctgat cttgtggaat cttttcaatg attttgccct tcttgatcaa taagccttct
     2941 tttttgccac ctgcaatcgc aatatccgca tgtttagctt caccaggacc attgaccgca
     3001 caacccatga tggctactgt tacttcttta tgaatcgttt gaagatagct ttcaatttca
     3061 ttgacaactg gctccatatc ccattgagtt cgtccacatg ttggacatgc gattagattg
     3121 ggtacatttt tgatgagctt gcagtctttt aataattctt ttacgattgg aatttctttt
     3181 tcaggtgcac cattaacact gattcgaatt gtatttccaa tgccttcatt taataaagtg
     3241 cctaaagcta aactcgattt gatggcactt gtcatggttg taccggcttc tgttacacct
     3301 aaatgtaaag ggtaatcaaa tgtcttagct gcaagacggt aagcttcaat acatagtagt
     3361 ggatcactcg atttaaaaga aaggacagta tcataaaaat cgagatcttc taagattttt
     3421 acatgtcttt tggcactttc aatcatacct tgtgcggttg gcttaccata tttttcatga
     3481 atatcttttt ctaaagaacc accattgatc cctatacgaa tgggaatatg tttttcttta
     3541 caagccttaa cgactttttc tacattttct ctagaaccaa tatttccggg atttaaacga
     3601 atcttatccg cacctgcttc aatacaagct aaagctaaac gataatcaaa gtgaatatcg
     3661 gcaactaatg gaatatgaat ttgttttttt atatctttta tggctttggc atcttccata
     3721 tccatgcagc tgacacgaat taattcgcag cccatttttt caaggtttaa aatttgtttg
     3781 gctacctcat ccgccttttt tgtttcaatg ttacacattg attgaataat aacgtgatta
     3841 ttatgtccaa tttcaagatt ttttacttga acttgacgag tttgagttct gttcatagtc
     3901 atcctccata tctcaagtat acgcctttct ttcttttttt tccatttcat cttggaaacg
     3961 tgtataatat atagtgtttt cgtataaa
//
LOCUS       DBMDWL010000180         3967 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_9848_length_3967_cov_2.454475, whole genome shotgun sequence.
ACCESSION   DBMDWL010000180 DBMDWL010000000
VERSION     DBMDWL010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9848_length_3967_cov_2.454475"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..>506
                     /locus_tag="ACZPDN_10160"
     CDS             29..>506
                     /locus_tag="ACZPDN_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299023.1"
                     /translation="MGTLFMMVIFLGALAGDGWLIYYFFKEILSDFAKEPSIEMMFLC
                     MPIAFVATLATIWLGYISSWFVAVDSFVNGGPYVPDFYDFAFSGKGFIYSCKISLITP
                     VLAATILISVEVYNKFCNCNLDDFKKFLLRLILIDLTTSVCINAFINSSLMVLLKTF"
     assembly_gap    507..516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <517..1153
                     /locus_tag="ACZPDN_10165"
     CDS             <517..1153
                     /locus_tag="ACZPDN_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299024.1"
                     /translation="KTIKKIETQKRIIKSTSRKINTYTANYLFADKVVDLFGVLDTSE
                     SQKLLIETKEKAKKRDYNQLCISVRKQWKKSKLKKIFNDKFIENKAVVYMLGILKECD
                     NSNNTAILIKNYEKLKKNAEFSRVPQLPRKINVKKQLDISSIVNFNNAITNEIKNKDR
                     DNTMLKNKDFFEGIAERYLHLKKKSIIFNTTLILLCLVGLAYNVLIWKGMF"
     gene            1253..2617
                     /locus_tag="ACZPDN_10170"
     CDS             1253..2617
                     /locus_tag="ACZPDN_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299025.1"
                     /translation="MAWVDIVKQRDAALNLVAEIAMHQADMNYILILNRNKTIRRKIH
                     MSGPKTSAYTVTSEVRRKLLEQAKLEQQRQLEEQQRIERENKKAEEQKKIKNLLVEIA
                     ALPDSLEEACNIAEIQQKTYGIGKQFLNKVSLLKEDIKSEIKENSNQDLTLEQLLEVK
                     QQLYVAKKAFASRIASMQKEGLSIHKELSDNLEKDIENGFKINIPRYNEHFVVQADQA
                     QKQSIDFSSEISDVKDELLALTTKCSKTLKEEIIKAITSLEEITEYDFFQNFKSITVH
                     RIKKEYEIEQRQIQQKFLEAQLYQKEQSYISDTIDEVMEEMGYTIIGNREVTKKSGKR
                     FRDELYTFSEGTAVNIRYDSQGKIAMELGGIDSTDRLPSSLEALKLENEMVMFCDKFT
                     ELEKRLETKGIVCKNRISHLPPKAEYAQIINTSDYDMETEPEIFKAECRNIKGNRKKQ
                     HRNE"
     gene            2623..3216
                     /locus_tag="ACZPDN_10175"
     CDS             2623..3216
                     /locus_tag="ACZPDN_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012708.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR1299026.1"
                     /translation="MEKYKVCPGCGEHNNPIFIECQNCGTDLMSVKVVDEATEKIVYE
                     NISEPEMVKICECGAENPAAARKCGSCGEDISDILPIPKTVNYCKQYVLSEISGTYTY
                     DVPNNFEIIIGRDNVLKEFIYDKSYVSRSHAKIISKYGNLYISNLSGTNFTYVNNKKI
                     SNDTPTLLKVDDEIGLGGCVSNGHRQDLAVYLVVREK"
     gene            3213..3779
                     /locus_tag="ACZPDN_10180"
     CDS             3213..3779
                     /locus_tag="ACZPDN_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024747.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HIR1299027.1"
                     /translation="MMYIARILYPVEVLGPGKRVGIWFCGCSHRCKGCSNPELWKRQE
                     KYRVSLESLLELIYKISDNNMIDGFTITGGEPMEQSEELMLLLENLKAISDDILVYTG
                     FKIEWLTEKQLQNISVLIDGKYIEELNVNSLLRGSSNQKIYILEKNQKDKYEMYLNTE
                     TNKIQNFTTNDGIVSVGIHKPDFMKEAK"
     gene            3780..>3967
                     /locus_tag="ACZPDN_10185"
     CDS             3780..>3967
                     /locus_tag="ACZPDN_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299028.1"
                     /translation="MSDFIPKWHKELEIFSKIKPLIIIEGNVLDVYSYPEDGSISKGS
                     ILRLTTYMHYFFKDLGYR"
BASE COUNT         1522 a          533 c          759 g         1143 t
ORIGIN      
        1 atagagagat tacatcggga ggttttgtat gggtacttta tttatgatgg tcatattttt
       61 aggagcatta gctggtgatg gttggcttat atattatttt tttaaagaaa tattatcaga
      121 tttcgcaaaa gaaccatcaa ttgaaatgat gtttctttgt atgcctattg cgtttgtcgc
      181 aacattggca actatttggc tggggtatat ttcttcatgg tttgtggctg tagatagctt
      241 tgtaaatggt ggtccatacg tcccggattt ttatgacttt gctttctctg gaaaaggatt
      301 tatttatagt tgtaagattt ctcttatcac tccagttctt gctgctacca ttttgataag
      361 tgtagaggta tacaataaat tttgtaattg caatttagac gattttaaaa aattcttatt
      421 aagattgata ttaattgatt taacaacatc cgtatgtatt aatgcattca taaatagtag
      481 cttgatggtc ttattaaaaa catttgnnnn nnnnnnaaaa actataaaga aaatagaaac
      541 gcagaaaagg attattaaat ctacatcacg taagataaat acatacacag caaattattt
      601 atttgctgat aaggtagttg acctatttgg tgtattagat acttccgaga gtcaaaagtt
      661 attaattgaa acaaaagaaa aagcaaaaaa gagagattat aatcaattgt gtataagtgt
      721 gagaaaacaa tggaaaaaaa gtaagttaaa aaagattttt aatgacaaat ttattgaaaa
      781 taaggccgtt gtttatatgc ttggtatttt aaaagagtgt gataatagca acaatacagc
      841 aattctcata aaaaactatg aaaaactcaa aaagaatgcg gaattttcga gagtgcctca
      901 actaccgaga aaaataaatg taaaaaaaca gcttgatata tcttcaatag taaattttaa
      961 taacgcaata acaaatgaaa ttaaaaataa agatagagat aatacaatgt taaaaaacaa
     1021 agattttttc gaaggtattg cggaaaggta tttgcattta aagaaaaaaa gtattatttt
     1081 taatacaacg ctaattcttt tatgtcttgt tggattggct tataatgtgc ttatttggaa
     1141 aggaatgttt taaaaatgac attagataaa tttaaaaagt tagaaaccgg tgattattct
     1201 gctatatgcc catatgaagg tgagtgtact attcacataa atagaattcc taatggcatg
     1261 ggtggatatt gtcaagcagc gggatgcagc attgaatctt gttgccgaga ttgctatgca
     1321 tcaagcggat atgaattata ttttgatatt aaacagaaat aagacaattc ggaggaaaat
     1381 tcatatgtct gggccaaaaa catcagcata tactgtaaca tcggaagtca gaaggaaatt
     1441 attggagcaa gcaaaattag aacaacaaag acaattggaa gagcaacaac gaattgaacg
     1501 agaaaataag aaagctgaag aacagaaaaa aataaaaaac ctgctcgtcg aaattgcggc
     1561 attgcctgac agccttgaag aagcatgcaa tattgcagaa atacaacaaa aaacatatgg
     1621 cataggcaaa caatttttga ataaagtgtc attgcttaaa gaagatataa aaagtgaaat
     1681 taaagagaat tctaatcaag acttgacact tgaacagtta cttgaggtta aacaacaatt
     1741 gtatgttgcg aaaaaagctt ttgcgtcaag aattgcaagc atgcaaaaag agggactatc
     1801 aatacataaa gagctgtccg ataatttaga aaaagatatt gaaaatggat ttaaaattaa
     1861 cataccaagg tataacgaac attttgtggt tcaagcggat caggcacaaa aacaatctat
     1921 agacttttca tctgaaatat cagatgtcaa agatgaactt ttagcactca ccacaaagtg
     1981 ttcaaaaaca ttaaaagagg aaatcataaa ggcaataaca agtcttgaag aaataacaga
     2041 gtacgatttt tttcaaaatt ttaaaagtat tacggttcat cgaataaaaa aagaatacga
     2101 gattgagcaa cgacaaatac agcaaaaatt tcttgaggca caattatacc aaaaggaaca
     2161 atcctatatt tccgatacca ttgacgaagt tatggaagaa atgggttata cgataatcgg
     2221 aaacagagaa gttacaaaga aaagcggcaa gcgtttccgc gatgagttat atactttcag
     2281 tgaaggtact gctgttaaca ttcgctatga cagccaaggt aaaattgcaa tggagcttgg
     2341 cggcatagac agcacagatc gccttccttc ttcgttggag gcgttaaaac ttgaaaatga
     2401 aatggtaatg ttctgcgaca aatttactga attagaaaaa cggcttgaga caaaaggtat
     2461 tgtttgcaaa aacagaatta gtcatctccc tcccaaggct gagtatgcac agattataaa
     2521 cacctcagat tacgatatgg aaacggaacc cgaaatattt aaagcagaat gcagaaatat
     2581 aaaaggcaac agaaaaaaac agcatagaaa tgagtgatac atatggaaaa atacaaagtg
     2641 tgtccgggtt gtggcgaaca caacaatccg atatttatag aatgtcaaaa ttgcggaacg
     2701 gatctgatgt ccgtcaaggt tgtagatgaa gcaacagaaa aaattgtata tgagaacatt
     2761 tctgaacccg aaatggtgaa gatatgcgaa tgtggtgcgg aaaatccggc tgccgcaagg
     2821 aagtgcggtt cctgcggcga ggatatttct gacattttac ccataccaaa aactgtaaat
     2881 tattgcaaac aatatgttct ttccgagatt tcgggaactt atacatatga tgttcctaat
     2941 aattttgaga ttattattgg cagagataat gtgctgaagg aatttatata cgataaatct
     3001 tatgtaagca gaagtcatgc aaagatcatt tcaaaatatg gtaatcttta tatatcaaac
     3061 ttaagcggta caaacttcac ctatgtaaat aataagaaaa tatcaaatga cacacctacc
     3121 ctccttaaag tagatgatga aataggactt ggtgggtgtg ttagcaatgg gcatcgacaa
     3181 gatttggctg tctatcttgt cgtgagggaa aaatgatgta tatcgcaaga atattatatc
     3241 cggttgaagt tttaggcccg ggcaaacgcg ttggtatttg gttttgtgga tgttcccaca
     3301 gatgcaaagg atgtagtaat ccagaactgt ggaaaagaca agaaaaatat agagttagtt
     3361 tagaatcttt attagaattg atttataaaa tatcagataa taacatgatt gatgggttta
     3421 caataaccgg cggtgagcca atggaacaat cggaagagtt aatgcttctg ttggagaatt
     3481 taaaagctat aagcgatgat attttggttt ataccggctt taaaattgaa tggctgacag
     3541 aaaaacaact tcaaaatata tcagtcctga ttgatggaaa gtatatagaa gaattaaatg
     3601 tcaattcatt gctacgaggt tcttcaaacc aaaaaatata tattttagaa aaaaaccaaa
     3661 aagataagta cgaaatgtac ttaaacacgg aaaccaacaa aatacaaaat ttcactacaa
     3721 atgacggtat tgtatcagta gggatacata agccagattt tatgaaggag gctaagtaag
     3781 tgagtgactt tattccaaaa tggcataaag aattagaaat cttcagcaag ataaaacctt
     3841 taataattat tgagggtaat gttcttgatg tttatagtta tccagaagat ggcagtattt
     3901 caaaaggaag tatattaaga cttactacgt atatgcacta tttcttcaaa gatttaggct
     3961 atcgcaa
//
LOCUS       DBMDWL010000181         3914 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_10066_length_3914_cov_2.349331, whole genome shotgun sequence.
ACCESSION   DBMDWL010000181 DBMDWL010000000
VERSION     DBMDWL010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10066_length_3914_cov_2.349331"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="ACZPDN_10190"
     CDS             complement(<1..348)
                     /locus_tag="ACZPDN_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnmM family RNA methyltransferase"
                     /protein_id="HIR1299029.1"
                     /translation="MKSMVDLSHEFIRPVLHKQAICVDATLGQGKDTDFFLSQNVHKV
                     YGFEIQRDVFESTKKRLGNQRTCFYNVGHEYMEEYIHEEVDAILFNFGYFPKGDPKIT
                     TQSSSSVSAVRQAL"
     gene            complement(345..872)
                     /locus_tag="ACZPDN_10195"
     CDS             complement(345..872)
                     /locus_tag="ACZPDN_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR1299030.1"
                     /translation="MISTLKGNIALLDFDLIVDPTNQQVLPMQGVSAQIFHQAGSEMM
                     KACQELNGLEVGKAKMTKAFNLPCKAVIHTCGPRYMDGTHNEDEYLAACYWNSMALAY
                     DYMRRNDMESINIAFPCISTGINAYPNHEACVIAIQTVKRLMNKFPETKAIHVCFVCD
                     KTEDYMLYKEALRLR"
     gene            complement(869..1750)
                     /gene="rmuC"
                     /locus_tag="ACZPDN_10200"
     CDS             complement(869..1750)
                     /gene="rmuC"
                     /locus_tag="ACZPDN_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR1299031.1"
                     /translation="MEIIILILLSIVIVCLIVLLMQNKSKESVSSNSDSLILQKNFEY
                     LLKQVQDVQKNQATSKMSLNQMEDYLYRMNQVMTNTKLRGNWGEYQLDMLLSVYCGQN
                     PSIYSMQYTLPNGKIADCAFHLPDTNKVLCIDSKFPMENYLNLQEDMDTYLRPFKMNM
                     KKHIDDVANKYINIDTMPYALLFVPSEAIYQFVCAKCDDLFTYALQKHVMLVSPTTLV
                     AEVMTLLSSTKDFYRTSHMEEIERNILLLQEDANRLVERSIKAEKTLETLATQFHAVS
                     ISAQKLSSRMTKMGEDE"
     gene            complement(1835..2584)
                     /locus_tag="ACZPDN_10205"
     CDS             complement(1835..2584)
                     /locus_tag="ACZPDN_10205"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1299032.1"
                     /translation="MRLERMSWKDCEEYFKNHDMVILTTGSTENHGIHNPLGTDTMIP
                     NKILELVEKQSDVLICPTLPYGDADFHTEYTGTISIGSDLLEMVMERISDRLYDCGAR
                     HFVFLNGHGGNSHALSKVCRHLYKKGAVGTIFDWWTIAGKINPKWAGGHGAGQETAAM
                     LYVDESLVQKDKIEDSTLKVFTPSLTASNAQTVNFKGVDIPVPRSNRLSSDNGWFGKD
                     HPKYATYEWGKEMVEATADFLVEYMKEFQKM"
     gene            2641..3147
                     /locus_tag="ACZPDN_10210"
     CDS             2641..3147
                     /locus_tag="ACZPDN_10210"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1299033.1"
                     /translation="MDIQILLFIQENIRHYILTPFFTLITNEYFLYILLGIVLIYMFK
                     KNEDTKRLAKETIIAFIICTILFVVLKLIFHRPRPFDAFNELIPLVDKPSDYSFPSGH
                     TASAFICAFMVYDGLPKKYSILIIILAILVAFSRLYVGVHYPTDILAGIILAYIVYKF
                     MKKTMSTK"
     gene            3225..>3914
                     /locus_tag="ACZPDN_10215"
     CDS             3225..>3914
                     /locus_tag="ACZPDN_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1299034.1"
                     /translation="MLHEVSFTSYNERDQVQGWIYVPACKPNGIVQLIHGFGEHSRRY
                     IHMISAFLDAGYIVAADDHVGHGKTAMVNNVWGDWGDKGCHTMMEDEHTLKKIVCEKY
                     PNLPYFLFGHSMGSFITRDFIAKYGNELNGATICGTTGIFRSAKEVKEKLKEIIDAGH
                     GEESDPNIVSSLMGWMCERCGEISIGNEWICHDPYVQKDHAQDPFDAFTRPTSNRSIY
                     DFIQMMLVIEGT"
BASE COUNT         1267 a          767 c          562 g         1318 t
ORIGIN      
        1 taaagcttgt ctaactgcac ttacacttga agaagattgg gttgtaattt taggatctcc
       61 tttaggaaaa taaccaaaat taaagagaat cgcatccact tcttcatgaa tatattcttc
      121 catatattca tgtccaacat tatagaaaca agttctttga ttccctaatc gtttttttgt
      181 actttcaaaa acatcccttt gaatttcgaa gccatatact ttatgcacat tttgcgacaa
      241 aaagaaatct gtatcttttc cctgtcctaa tgtagcatct acacatattg cttgtttatg
      301 aagaactggc cttatgaatt cgtgggataa atcaaccata gatttcatcg cagtctaagt
      361 gcctctttat ataacatata atcttctgtc ttatcacata caaaacatac atgaatcgcc
      421 tttgtctcag gaaacttatt cattaatcgc tttacagttt gaatcgcaat tacacaagcc
      481 tcatgatttg gatatgcatt gatacctgta gaaatacatg gaaaagcaat attaatcgat
      541 tccatatcat ttcttcgcat ataatcataa gccaatgcca tactattcca ataacaagcg
      601 gccaaatatt catcttcatt atgcgttcca tccatatagc gtggtccaca cgtatgaata
      661 actgccttac atggtaaatt aaaagctttg gtcattttag ctttcccaac ctctaaacca
      721 ttcaattctt gacatgcttt catcatttca cttccggctt gatgaaatat ttgtgcacta
      781 actccttgca tcggtaatac ttgttgattg gttggatcga caatcaaatc aaaatccaac
      841 aaagctatat ttccctttaa tgtactaatc attcgtcttc tcccatctta gtcattcgac
      901 ttgataattt ctgtgcagaa atagaaaccg catgaaattg cgttgccaaa gtttccaatg
      961 ttttttctgc ctttatactt ctttccacaa gacgattcgc atcttcttga agcaataata
     1021 tatttctttc gatttcttcc atatgacttg ttctataaaa atctttagta cttgataata
     1081 atgtcatcac ctctgctaca agtgtcgttg gtgatactaa catcacatgt ttttgaaggg
     1141 catatgtgaa taaatcatcg cattttgcac aaacaaattg ataaatagct tcactaggca
     1201 caaacaataa ggcataaggc atcgtatcta tattgatata tttattagcc acatcatcaa
     1261 tgtgcttttt catattcatc ttaaaaggcc ttaaatacgt atccatatct tcttgtaaat
     1321 ttaaatagtt ttccattgga aactttgaat caatacacaa tactttatta gtatctggca
     1381 aatgaaaagc acaatccgca atctttccat tcggcaaagt atactgcata gaataaatgg
     1441 atggattttg accacaatat acacttagaa gcatatccaa ttgatattct ccccaatttc
     1501 ctcttaattt tgtgtttgtc ataacttgat tcatacgata caaatagtct tccatttgat
     1561 tcaaactcat tttagatgta gcctgattct tttgaacatc ctgtacttgt tttaaaagat
     1621 attcaaaatt cttttgaaga atcaaagaat ccgaattcga agaaacactt tcctttgatt
     1681 tattttgcat caacaaaaca attaaacata caatcacaat acttaacaat attaaaataa
     1741 tgatttccat taaaccctcc acacgaaaag aaaagagaca aaaatatttg tcccctattt
     1801 gtatatttat tataattcaa cttcaaatga attactacat cttttgaaat tctttcatgt
     1861 attccacaag aaaatcagcc gtagcctcaa ccatctcttt accccattca tatgtcgcat
     1921 atttaggatg atccttgcca aaccagccat tatcactact taaacgattg cttctaggaa
     1981 caggaatatc tacaccttta aagttaaccg tttgagcatt agaagctgtt aaagatggag
     2041 taaaaacctt taatgttgaa tcttcaatct tatccttctg tactagactt tcatccacat
     2101 acaacatagc ggccgtttcc tgacctgcac catggcctcc agcccatttt ggattaattt
     2161 ttccagcaat ggtccaccaa tcaaaaatcg tacccacagc tcccttttta tataaatgac
     2221 gacacacttt acttagtgca tgactatttc ctccatgtcc atttaaaaat acaaagtgtc
     2281 tagcaccaca atcataaagt cgatcactaa ttctttccat gaccatctct aataaatcac
     2341 taccaattga tattgtacct gtatattctg tatgaaaatc cgcatctcca taaggcaatg
     2401 ttggacaaat caatacatca ctctgttttt ctacaagctc taaaatctta ttaggaatca
     2461 tagtatcggt tcctaatgga ttatgtattc catgattttc tgtacttccc gttgtcagaa
     2521 tcaccatatc atgattctta aaatattctt cacaatcttt ccaactcatt ctttctagtc
     2581 gcatagcttc acttcctatc tgtatatatg tataatactc ctaacgaaag gaaaatttcc
     2641 atggatattc aaattttatt atttatacaa gaaaacattc gtcactatat actcacacca
     2701 ttcttcactt tgatcacaaa cgaatacttt ttatacatac ttctaggaat cgtattgatt
     2761 tatatgttca agaaaaatga agatacgaaa agactcgcaa aagaaacaat cattgccttt
     2821 ataatttgta ctatattatt cgttgtatta aaactcatat ttcatagacc tagacccttt
     2881 gatgcgttta acgaacttat tccattagtg gataaaccta gtgattattc atttccatct
     2941 ggacacaccg catctgcctt tatttgtgcc tttatggtgt atgatggtct tcccaaaaaa
     3001 tacagtattc taatcatcat actcgcaatc cttgttgcgt tttctagact ctatgtaggc
     3061 gtccattatc caacggatat tcttgcaggt attattcttg cgtacattgt ttataaattc
     3121 atgaaaaaga ctatgtcaac taaatgacat agtttttttg tgggcgtata ataaaaacag
     3181 atataaaacg agttaacatt acgcaaaaaa aggaggatta ttgtatgtta cacgaagtat
     3241 cttttacatc ttataatgaa cgcgatcaag tgcaaggttg gatttatgtt cctgcatgca
     3301 aaccgaatgg aattgttcaa ttgatccatg gatttggcga acattcaaga cgctatatcc
     3361 atatgatttc ggcttttcta gatgcaggct atattgtagc cgctgatgat catgtaggtc
     3421 atggaaaaac cgcaatggta aataatgtct ggggtgactg gggagacaaa ggatgccaca
     3481 ccatgatgga agatgaacac acattgaaga aaattgtatg tgagaaatat ccaaatttgc
     3541 cttacttctt atttggtcac agtatgggtt catttatcac aagagatttt attgcgaaat
     3601 acggaaatga attaaatggt gcaacaattt gtggtacaac aggcattttc cgtagtgcta
     3661 aagaagttaa agaaaaattg aaagaaatca ttgatgcagg acacggtgaa gagtcggatc
     3721 ctaatattgt tagctcactt atgggctgga tgtgcgaacg ttgtggtgaa ataagtattg
     3781 gaaatgaatg gatttgccat gatccatatg tacaaaagga ccatgcacaa gatccttttg
     3841 atgcctttac aagacccaca agcaatcgtt ccatttatga ctttatccaa atgatgcttg
     3901 tgattgaagg aacg
//
LOCUS       DBMDWL010000182         3842 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_10349_length_3842_cov_2.464582, whole genome shotgun sequence.
ACCESSION   DBMDWL010000182 DBMDWL010000000
VERSION     DBMDWL010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10349_length_3842_cov_2.464582"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            97..1113
                     /locus_tag="ACZPDN_10220"
     CDS             97..1113
                     /locus_tag="ACZPDN_10220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIR1299035.1"
                     /translation="MNYKELMEKSREVMGTKLCKSCPICDGKACRNTIPGPGAKGIGD
                     VAMRNYDAWKNIRVNMDTITDNEPVSTELELFGHTFKYPIFAGPVGAVGMHYSDAYDD
                     NSYNDILVRGCMNAGICAFTGDGKDPNIMINATRIIKENNGYGIPTIKPWSLETYLEK
                     LDLALNSNAFAVAMDVDAAGLPFLKGCQPPAGRMNTEQLKAIISNTPVPFIVKGVMSV
                     KGALKAKEAGASAIVVSNHGGRIQDQTFATAEVLEDIVKAVGGSMKIFVDGGLRNGVD
                     IFKALALGADAVIVARPFVNAIYGAKEEGIQVLVNKLGSELQDTMEMCGAKSLKDITR
                     DMVG"
     gene            1257..2603
                     /locus_tag="ACZPDN_10225"
     CDS             1257..2603
                     /locus_tag="ACZPDN_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299036.1"
                     /translation="MSEEEKKVPLEDGNMKEVKEGEEEQQKENSTEIPPVPEENVENT
                     NVETEKQKDVKNKIADFIKGHKKMVAIIVALIIVFILMTSSSKIVGKDLTYDIGDTVT
                     LDVDHLVDKDATDVDLTDIEIESELISDTSSYTYNSYKNTVITKGLNCLDAGTYEVTL
                     KDKSNESVKDTIEITVVDKKAPKFVDFKDVVEVDEGDTDFSLKDAFEAKDDSKVSISV
                     DKGNFNVNKKGEYKIKVTAKDTSGNKITKKATVKVVQKVLSGIEATYNGKTGEGTTID
                     KSSNIKVTAKYEDDSSKEVYDWTIDNPATLKADETSTVTVKYKDKTYDLLVQCSTVGE
                     QGFKKQCQNIAYEELARNGNSHIGEKVKFYGQVLQVMNGSGNSVTLRVSTKSSAYGNW
                     YDDVVLVEYEYKSGQPKFLEDDMITFYGYVYGDYSYEAVSGATITIPAVLASYIDM"
     gene            2861..3754
                     /locus_tag="ACZPDN_10230"
     CDS             2861..3754
                     /locus_tag="ACZPDN_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024571.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1299037.1"
                     /translation="MKRNAILKLVQWKNSPERKPMVLRGARQVGKTWLMKEFGKNYYD
                     NYVYFNFDEEDELKSIFETNKNPHRIIELLSMISDEKIEPEKTLIIFDEIQECPEVLN
                     TLKYFKEKANEYHVITAGSLLGTLLAKPKSYPVGMVNLLDIYPLTFDEFLNAIDSGLY
                     AYYESIQKEQVIEQIFHQRLLDAYNYYLIIGGMPECVSSWIKYKDPTMISRIQRELVE
                     VYENDFSKHNGKVNSGRILMVFRSIVAQLAKPNEKFMYGAVREGARARDFEEAIEWLV
                     SAGMLNRVYNVSKMEHPRSLN"
     gene            complement(3781..>3842)
                     /locus_tag="ACZPDN_10235"
     CDS             complement(3781..>3842)
                     /locus_tag="ACZPDN_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025645.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HIR1299038.1"
                     /translation="DGSREQEVEIYYRFIGKID"
BASE COUNT         1394 a          441 c          839 g         1168 t
ORIGIN      
        1 agccgtatgc gggaaaactg cacgtacggt tcttagagga gtttatctcc aactgtttaa
       61 agtaaatatg agtaaaagaa ggaggtaaat ttacttatga attataagga attgatggaa
      121 aaaagtcgtg aagtgatggg gacaaaatta tgtaagtctt gtcctatatg tgatggaaag
      181 gcatgtcgaa atacgattcc aggtcctggg gctaaaggga ttggggatgt tgcgatgcgt
      241 aactatgatg cttggaaaaa cattcgtgta aatatggata caattacaga caatgagcca
      301 gtaagtactg aattagaact atttggtcat acatttaaat atccgatttt tgcagggcct
      361 gtaggagctg taggaatgca ctattcagat gcatatgatg acaatagcta taatgatatt
      421 ttagttcgag gttgtatgaa tgctggtatt tgtgcattta caggagatgg aaaagatcca
      481 aatattatga tcaatgcgac gcgtattatt aaagaaaata atgggtatgg tattccaacc
      541 attaaacctt ggagtttaga aacatattta gaaaaattag acttagcttt aaatagtaat
      601 gcctttgcgg tagctatgga cgtagatgca gctggacttc ctttcttaaa ggggtgtcag
      661 cctcctgcag gaagaatgaa tacagaacaa ttaaaggcta tcatttcaaa tacacctgta
      721 ccatttattg tgaagggtgt catgagtgtg aagggcgctt taaaggcaaa agaagcagga
      781 gctagcgcaa ttgtggtttc gaatcatggt ggtcgaattc aagatcagac ttttgcgaca
      841 gctgaagtgc ttgaagatat tgtaaaagcg gttggtggaa gtatgaaaat atttgtcgat
      901 ggtggacttc gtaatggtgt cgatatcttt aaggctttgg cattaggtgc agatgccgtc
      961 attgtagctc gtccatttgt gaatgcgatc tatggagcaa aagaagaagg tattcaagtc
     1021 ttagtgaata aacttggtag tgaactgcaa gatactatgg agatgtgtgg ggctaaaagc
     1081 ttaaaagaca ttactcgtga tatggtagga tagtttaaat gaagattaag atttagttgt
     1141 tgaaataatc ttaatctttt tttgcgctca ttttggatag ataattagca attgattatt
     1201 gataaaaaat ggggggtatc attaaaatag aaaggttata ttttagggtg aaaaatatga
     1261 gtgaagaaga aaagaaggta cctcttgaag atgggaatat gaaagaggtg aaggaaggag
     1321 aagaagaaca acaaaaagaa aattcaacag agattcctcc agtacctgag gagaatgtag
     1381 aaaataccaa tgttgaaacc gaaaaacaaa aagatgtaaa gaataaaata gctgatttca
     1441 tcaaaggtca taaaaaaatg gttgctatta ttgtggcatt gataattgtt tttattttaa
     1501 tgacgtctag ttctaagatt gttggcaaag acttaacata tgatattgga gatacagtga
     1561 cattggatgt cgatcatttg gtagataaag atgcaactga tgtagatttg actgatatcg
     1621 aaatagaaag tgaattgatt tcggatacta gttcttatac gtataatagt tataaaaata
     1681 ctgtaattac taaaggattg aattgtttgg atgctggaac ttatgaagtt acattaaaag
     1741 acaaatctaa tgaaagtgtt aaggatacaa tagagattac agtagttgat aaaaaagctc
     1801 ctaaatttgt ggattttaaa gatgtagtag aagtagatga aggagacaca gatttttcgt
     1861 taaaagatgc ctttgaagca aaagatgatt caaaagttag cattagtgta gataaaggca
     1921 actttaatgt taataaaaaa ggtgaatata agattaaagt aacagctaag gatacttctg
     1981 gtaataaaat aactaaaaag gctactgtaa aagtagtgca aaaagttctt tctggcattg
     2041 aagctactta taacggtaaa acaggcgaag gaactacaat tgataaatct tcaaatataa
     2101 aggtaactgc aaaatatgaa gatgacagta gtaaagaagt atatgattgg actatagata
     2161 accctgctac tttaaaagca gatgaaacta gcacggtaac cgtaaaatac aaagataaaa
     2221 cttatgattt gttagtacaa tgtagtactg ttggagaaca aggatttaag aaacaatgtc
     2281 aaaatatagc atatgaagaa ttggctagaa atggaaattc tcatattgga gaaaaggtta
     2341 aattttatgg gcaagtatta caggtgatga atggtagtgg aaattctgtt actttacgtg
     2401 tctcaacaaa atctagtgct tatggaaatt ggtatgatga tgtagtttta gtagaatatg
     2461 aatataaatc aggtcaacca aaattcttag aggatgatat gattacgttc tatggctatg
     2521 tatatggtga ttatagttac gaggctgtaa gtggagcaac aattacaatt ccagctgtat
     2581 tagcatcata cattgatatg taaaatgaat atgataaaaa ctgtaacaaa agttgtgtta
     2641 cagtttttta aaccctgtga taatcggatt gcgttagaaa atacattttt taagctgtta
     2701 aaatcacaga aaaatcgata aaaaggaaaa atgtgtaaaa tagatactaa aaacctcaga
     2761 aaaatgtgtt aaatcaatta taaaaagact ggaaaaatgt gttaaatagg tatagaaaat
     2821 cgtggaaaaa tgtgttaact taaattcaag aggtggtttc atgaagcgaa atgcgatttt
     2881 aaagctagtt caatggaaaa atagtcctga gagaaaacct atggtattaa gaggtgcaag
     2941 acaggttggt aagacttggt tgatgaagga atttggtaag aattattatg ataattatgt
     3001 ttattttaac tttgatgaag aagatgaatt aaaatctatt tttgaaacca ataaaaatcc
     3061 acatcgtatt attgaacttc tttctatgat cagtgatgaa aaaattgagc ctgaaaaaac
     3121 tttgattata tttgatgaaa ttcaggagtg tcctgaagta ttaaatacac tgaagtattt
     3181 caaagaaaag gcaaatgaat atcatgtgat aacggcaggt agtcttttag gcacattatt
     3241 ggcaaagcca aaatcatatc ctgtaggcat ggttaatctt ttagatattt atccgttgac
     3301 gtttgatgag tttttaaatg cgatagattc aggattgtat gcatattatg aaagtattca
     3361 aaaagaacaa gttatcgaac aaattttcca tcaacgctta ttagatgctt ataattacta
     3421 tcttattatt ggtggtatgc ctgaatgtgt atcttcatgg atcaagtata aggatcctac
     3481 catgatatct agaatccaaa gagaattggt tgaggtttat gaaaatgact tttctaagca
     3541 taatggaaaa gtgaatagtg gacgtatttt gatggtgttt agaagtattg ttgctcaact
     3601 tgcaaaaccg aatgagaagt ttatgtatgg tgctgtccgt gaaggagcta gagctagaga
     3661 ttttgaagaa gctattgaat ggctagtatc cgctggtatg ttgaatcgtg tatataatgt
     3721 gtctaagatg gaacatcccc gctcccttaa ttaaagatat tgttgggtaa aaaagaaagg
     3781 tcagtcgatt ttgccgatga agcggtagta gatttctact tcctgctcac ggcttccgtc
     3841 ct
//
LOCUS       DBMDWL010000183         3814 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_10475_length_3814_cov_2.550229, whole genome shotgun sequence.
ACCESSION   DBMDWL010000183 DBMDWL010000000
VERSION     DBMDWL010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10475_length_3814_cov_2.550229"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="ACZPDN_10240"
     CDS             complement(<1..440)
                     /locus_tag="ACZPDN_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299039.1"
                     /translation="MKIVKKKLDDMYRCYCASNIYFDNQNHILLASEDPDVACNMYYG
                     KDYETKENVWTHPGGCMSIVPIPGKEKEFLCVQEFYLKVTPSLAKIVWGKYDNGTWQF
                     KDVVSVPYVHRFGIFNQNGINYLILATVATSKKEKNDWSVPGRI"
     gene            complement(535..1596)
                     /locus_tag="ACZPDN_10245"
     CDS             complement(535..1596)
                     /locus_tag="ACZPDN_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HIR1299040.1"
                     /translation="MFKDVFKNFKSHCMTGISYMIPVIVIGGFCTAIARLCGNVDVQG
                     TLGYAFISAGNAAFGLMMSVLCAGIAYSICGKPGIAPGVVAGYLSTQVKASFLGALIC
                     GFLIGMMVLWMQEKFPDSKALKSMYPIVIYPVLSGIVSTLLIMFVFGPPLAALTQIIV
                     DFFMGMNTGSKFILGFLLGCMTGFDMGGPVNKICFSVVSAFAASGVWGPAAGKNAAAM
                     APPLGMAISALILTPKKYTAEEREDAKVAIAMSLCQVTEGALPFAFNDPKRVIPAVTI
                     GSGVAHGLVLAWGVTCPVLHGGVFSIPLTSNPLLWVAAYLIGAMVTAVIVSVLKPARP
                     VEAESEDEELKVDFDIEIG"
     gene            complement(1764..2081)
                     /locus_tag="ACZPDN_10250"
     CDS             complement(1764..2081)
                     /locus_tag="ACZPDN_10250"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HIR1299041.1"
                     /translation="MKIVGITSCTCGIAHTYMAREKLLETGKKLGWSVKIETQGSGGI
                     EFALTDQDIQEADCVLLATDVAVSGTERFKGKPVVKVPVSTAIKSPEHLLKKIEEKLS
                     AKK"
     gene            complement(2081..2569)
                     /locus_tag="ACZPDN_10255"
     CDS             complement(2081..2569)
                     /locus_tag="ACZPDN_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1299042.1"
                     /translation="MNLIEILDVNLIDLEMEASSKDEVIHQLCSLLYENGRVKDLEKF
                     KEAVYEREQIGETGMGNQIAIPHGLTDQVNKASVAIAKVKNPVEWESLDDQPVRLIFL
                     LAAPTNELEKTHLQMLAQLASVLAYKEHVDALMECETKEQFFKLFESYFDEFTKRKEE
                     NK"
     gene            complement(2587..>3814)
                     /locus_tag="ACZPDN_10260"
     CDS             complement(2587..>3814)
                     /locus_tag="ACZPDN_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012579.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HIR1299043.1"
                     /translation="ILKNKELEMLDCDELQEESHSYKEEFEQLIYELSEYIHSDIPKA
                     ESNYLLMHLFGMYLNEPTFIKNDFLSELRTLAENISDDFICNLDKIVALNLKQNKMFK
                     QSLVLHLLPTVYRLKYGFNLYNPLLGEIKTNYAGSFSLATIINSSFEKFLNVTVSEEE
                     IAYVALHISVAVEQPMEKQQVAIVCTMGVGVSRFLSVKLQENFPNITFIHCSSEDSQA
                     IENCNYILSTVKLSVSKPYLQINPLLNETDIQRIRTFIYGNPSVNKNNFSLQTVMVEH
                     GHTDRLTVLQEMTNRLQLCGAVTPYFLESVLKRETMGSTEVGNGIILTHGFYEAVKRT
                     QIAFYKLDNPIFWNTQKVNFIVLLAVAKVDAKNVMQMSWLYKTLNSESIIEQVHKCSC
                     EKELYELLINASKNNE"
BASE COUNT         1236 a          726 c          493 g         1359 t
ORIGIN      
        1 taaattcttc ctggaacact ccagtcattc ttttcttttt tagaagtagc tactgtagct
       61 aaaatcaaat agttgatacc attctggtta aagattccaa aacgatgaac ataaggtaca
      121 gacacaacat ctttgaattg ccatgttcca ttgtcgtatt ttccccatac gatttttgcc
      181 aaagatggcg taacctttaa atagaattct tgaacacaaa gaaattcttt ttcttttcca
      241 ggaattggaa cgattgacat acatccacct ggatgcgtcc atacgttttc ttttgtttca
      301 taatcttttc catagtacat attacatgcg acatctggat cttcacttgc caataaaata
      361 tggttttgat tatcaaaata aatgttactt gcacaatagc aacggtacat atcatctagt
      421 tttttcttaa caattttcat gattttctcc tctttttagg taattttggg tataagaaaa
      481 gctgcataaa cgcagctttc atttttttat tattttactg cgaattggtt tgatttatcc
      541 aatttcaata tcgaaatcaa ctttcaattc ttcgtcttca gattcagctt ctactggtct
      601 agctggtttt aaaacagata cgataacggc tgtaaccata gcaccaatta agtaagctgc
      661 aacccataac aatggattac ttgttaatgg aattgagaat acacctccat gtaataccgg
      721 acatgttact ccccaagcta aaaccaatcc atgggcaact ccagatccaa ttgtaacggc
      781 tggaataact cgtttaggat cattaaatgc gaatggtaaa gcaccttctg ttacctgaca
      841 taatgacatt gcgatagcaa cttttgcatc ttctctttct tctgcagtat atttctttgg
      901 agttaaaatc aaagcagaga tagccattcc taaaggtgga gccatggcag ccgcattttt
      961 accagcagca ggtccccaaa caccactagc agcaaatgca cttactactg agaaacaaat
     1021 cttattaaca ggacctccca tgtcaaagcc agtcatacaa cccaacaaga atcctagaat
     1081 aaatttagat ccagtattca ttcccataaa gaaatcaaca ataatttgag ttaaagctgc
     1141 taaaggtggt ccaaagacaa acataatcaa caatgtagaa acaattcctg ataatactgg
     1201 ataaataacg attggataca tagatttcaa agcttttgaa tctgggaatt tttcttgcat
     1261 ccaaagaacc atcataccaa tcaagaatcc acaaattaaa gcacctaaga atgaagcttt
     1321 tacttgtgtt gataaataac ctgcaaccac acctggtgca atacctggtt taccacaaat
     1381 tgaataagca atacctgcac ataatacgga catcattaat ccaaatgctg cattacctgc
     1441 tgaaataaat gcataaccta atgtgccttg tacatcaaca tttccacata aacgtgcgat
     1501 tgccgtacag aatccaccaa ttacaatgac tggaatcata tacgagatac ctgtcataca
     1561 atggctttta aagtttttaa aaacatcttt aaacattttt tctctcctat attatctatt
     1621 attcacttat attttcattg aagaacatgt tctattcata tataatgaac ttggttaaat
     1681 taaccaaaga acgattttat gtgaagaaca attaaaaggt gctcgccaaa gtttctttta
     1741 ttttcaatga agatcgtttt tttttacttt tttgcgctta atttttcttc aattttcttt
     1801 aataaatgtt ctggtgattt aattgctgtt gaaacaggaa ccttaacaac tggttttcct
     1861 ttaaaacgtt ctgttccaga aactgcaaca tcagtagcta ataaaacgca atctgcttct
     1921 tgaatatctt gatcagttaa agcaaactca attccaccac ttccttgagt ttcaattttt
     1981 acgctccatc ctagcttttt acctgtttca agcaattttt ctcttgccat atatgtgtgt
     2041 gcaataccac aagtacatga agtaattcct acgattttca ttatttattt tcctcttttc
     2101 tttttgtaaa ttcatcaaaa tatgattcaa acaatttaaa aaattgttct ttcgtttcac
     2161 attccatcaa agcatccaca tgctctttat atgctaatac acttgcaagt tgtgctagca
     2221 tttgtaaatg tgttttttcc aattcatttg ttggagcagc taataagaaa atcaatcgaa
     2281 ccggctgatc atctaagctt tcccattcta caggattttt aactttagca atcgcgacac
     2341 tcgctttgtt tacctgatct gttaaaccat gaggaatagc tatttgattt cccattcctg
     2401 tttcaccaat ttgttctctt tcatatactg cttctttgaa tttttctaag tcttttactc
     2461 ttccattttc ataaagcaat gaacataatt gatgaataac ttcatcttta ctagatgctt
     2521 ccatttctaa atcaattaaa ttgacatcta aaatttctat caaattcata caattaacct
     2581 ttcattttat tcgttatttt tacttgcatt gatcaataat tcatacaact ctttttcaca
     2641 actacattta tgtacttgtt caattattga ttcactattt aacgttttat acaaccaact
     2701 catctgcatt acgtttttcg catctacttt tgccacagca agcaaaacta taaagtttac
     2761 tttttgtgtg ttccaaaaaa tcggattatc taatttgtaa aaagcaattt gtgttctttt
     2821 cactgcttca tagaatccat gtgttaagat gattccatta ccgacttctg tagatcccat
     2881 tgtctctctt tttaatacac tctctaagaa ataaggagta acagctccac atagttgcaa
     2941 gcgatttgtc atttcctgca aaactgttaa tcgatctgta tgtccatgtt caaccatcac
     3001 agtttgaagc gagaagttat tcttattaac cgaaggattt ccataaatga aagttcttat
     3061 tctttgtata tctgtctcat ttaacaaagg attaatttgt aaataaggtt tagaaacgct
     3121 taattttaca gttgataaaa tgtaattaca attttcaatt gcttgcgaat cttctgaaga
     3181 acaatgaata aatgttatat ttggaaaatt ctcctgcaat ttaacagata agaatctaga
     3241 aactccaacg cccattgtac aaactatcgc aacttgttgt ttttccattg gctgttctac
     3301 agctacagaa atatgtaaag caacatatgc gatttcttct tcactaactg ttacatttaa
     3361 aaatttttca aaggaagaat tgataattgt agctaatgaa aaggatcctg cataatttgt
     3421 ctttatttct cctaataatg gattatataa attaaatcca tattttaatc gatacactgt
     3481 tggcaacaaa tgtaaaacca atgattgttt gaacatttta ttttgtttta aatttaaagc
     3541 aactatctta tccagattac aaataaaatc atcactaata ttttccgcca atgttcttaa
     3601 ttcgcttaag aaatcatttt taataaaagt tggttcattt aagtacattc caaataaatg
     3661 catcaacaaa tagtttgatt cagcttttgg aatatcagaa tgaatatact ctgatagttc
     3721 ataaattaat tgttcaaatt cttctttata agaatgagat tcttcttgca attcatcaca
     3781 atccaacatt tctaattctt tgtttttaag aata
//
LOCUS       DBMDWL010000184         3771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_10663_length_3771_cov_1.801362, whole genome shotgun sequence.
ACCESSION   DBMDWL010000184 DBMDWL010000000
VERSION     DBMDWL010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10663_length_3771_cov_1.801362"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="ACZPDN_10265"
     CDS             complement(<1..875)
                     /locus_tag="ACZPDN_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012694.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1299044.1"
                     /translation="MLSNNEKYIFTSELALAISNGLQVEDGLKMLVGFDADVSMCAKK
                     LEDIMKGSSFTDALKESKEFDEYMIQMVVVGQSIGNLDVVFKELSTYYARQKELNYQI
                     QDAITYPFVLILMMFVIVATLIFKVFPIFENILSQMSMSLSLMYTARILSYIGFFILL
                     FILVFGLILYFIYKKSPNKTWMTKLPFFSKLNYQKEMTKFTYILSLFVSSGYALIDAV
                     DIILLSIEHPVLKDKILHVKNRMLEGESLANALVKENVYDQGYGALLMAADQSGHQDE
                     VLKTLSNRYKEDLER"
     gene            complement(887..1792)
                     /locus_tag="ACZPDN_10270"
     CDS             complement(887..1792)
                     /locus_tag="ACZPDN_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR1299045.1"
                     /translation="MRKYRIVKNVVYILMAFCLLCGCQDSTKYPTKCEESPSQTETEV
                     LWPSQEELSVLSDGSLAEVDLTHANDGYICARLLQETSGIKLQISKDDKKYNYDLKTT
                     EFKPFPMNMDNGSYTIKILQQIEGTRYAICASNTIDVQLPNDLVPYLYPNQIVDYTKD
                     SYVYKKSFELVKDDRDDLTRIAHLFKYVVDTLDYDDQKAKDVSDTFVLPDIDGSLKTG
                     KGICFDYAASLAALCRIQGIPAKVIVGWTDIEYHAWVEIYLKDTGWINPKIYFDKEKW
                     NLVDPTFSDSKNSDYEGKYDEVYHY"
     gene            complement(1811..>3771)
                     /locus_tag="ACZPDN_10275"
     CDS             complement(1811..>3771)
                     /locus_tag="ACZPDN_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1299046.1"
                     /translation="NTFARNLNKEPDLDSSLFEMKKESDFSSIQFIDMNHNVYSSKKD
                     SKFSYNNLSDEYKDKIKNGFLGEQSTFKQKNKNYKLTYIVPVHGNENQIVGVLCADSS
                     LKPYTDLMRKSISGGNLYITDFKDFYGIQKNLESNEVLAVIKNMNLSENVNGLIGVKG
                     QEHGIVVKKIGIQGWYLCYVNSAKSLNYPLYVMSRTTNYVLMLFVIVVILLLWIAYRM
                     MVKNNEEMEKLAYTDQLTGAVSFARFTQLVRIYALKNNNYSLVSLNMRRFKFMNEILR
                     RDKSDDFLCRIVTCIKPMLKKDEIICRDSADVFYMLLMDTAENKVSNRIHAIFNSIRQ
                     NTKDFCYEYEFCCGVICNGARQEKYSFEQMLTNVMFALAQSKEMASENICFFDEEVHK
                     KKEFENFVESNMQQALDSNRFEMVLQPKVDLDTNLVVSAEALVRWKLEDGTYLPPSSF
                     VGIFEKNRFCSKLDFYMLRKAIQQIRKWIDMGIQPVNLSVNQSKIVFYHENYISELKS
                     LLKEYDVSASYITLEVLESTVIEDFKLFNQILLEVKKLGFSVSLDDFGSGYSSLNVLS
                     KLTIDELKIDRIFLHIDSDLENKKTKWILEGIINIAKKSNIRVVVEGVESSKDDEFIR
                     CLGANVGQGYYYSRPLSIDAFNDLIHKK"
BASE COUNT         1318 a          741 c          404 g         1308 t
ORIGIN      
        1 attctttcta aatcttcctt ataacgattt gataaggttt ttaatacttc atcttgatga
       61 ccactttgat ccgctgccat caataaagca ccatatcctt gatcatacac attctctttt
      121 actaatgcat ttgccaggct ttctccttca agcatacgat tctttacatg taaaatttta
      181 tcctttaata caggatgctc aatcgataga agtataatat ctacagcatc aatcaatgca
      241 tatccacttg aaacaaataa agataatata tatgtaaact tagtcatttc tttttgataa
      301 ttcaacttag aaaagaatgg taattttgtc atccatgttt tattcggact ttttttataa
      361 ataaaatata aaataagacc aaaaactaga ataaacaata aaataaaaaa gccaatataa
      421 cttaagattc gagctgtata catcaaagac aatgacatcg acatttgact caagatattt
      481 tcaaaaatag gaaacacttt aaatattaag gtggcaacaa tcacaaacat catcaaaatc
      541 aaaacaaatg gatatgtgat tgcatcttga atttgataat tcaattcttt ttgtcttgca
      601 taatatgtac tcaattcctt aaacactaca tctaaattcc caatactttg cccaactaca
      661 accatctgaa tcatatattc atcaaattct ttagattctt ttaatgcatc tgtaaaagaa
      721 gaacctttca ttatatcttc taattttttt gcacacatag atacatctgc atcaaaacca
      781 acaagcattt tcaagccatc ttccacttgc aaaccatttg aaatagccaa tgccaattca
      841 cttgtaaaaa tatatttttc attattcgat aacatacata cgcctcctaa tagtgataca
      901 cttcatcata tttcccttca taatccgaat tttttgaatc cgaaaatgta ggatctacaa
      961 gattccattt ttccttatca aaatatattt taggattgat ccatcccgta tcctttaaat
     1021 aaatttcaac ccaggcatga tactcaatat ccgtccatcc aacaatcacc tttgccggaa
     1081 taccttgaat tctgcataga gcagcaagac ttgccgcata atcaaaacaa attcccttgc
     1141 cagtttttaa tgaaccatca atatctggta agacaaacgt atcacttaca tcctttgcct
     1201 tttgatcgtc ataatctaat gtatctacaa catatttaaa caaatgtgct atacgcgtca
     1261 aatcgtcacg atcatctttt acaagttcaa aagatttttt atatacataa gaatcctttg
     1321 tataatcaac aatttgattc ggatataaat agggaacaag atcatttggc aactgaacat
     1381 ctatcgtatt agaagcacat atcgcatatc ttgttccttc tatttgctgc aaaattttta
     1441 tcgtataact tccattatcc atattcattg gaaaaggctt gaattctgtt gtctttaaat
     1501 catagttata ttttttatca tcctttgaaa tctgtagttt gattcccgaa gtttcttgaa
     1561 gtaacctagc acaaatatat ccatcatttg cgtgcgttag atccacttca gctagactac
     1621 catcactcaa aacactcagt tcttcctggc taggccataa aacttcagtt tccgtttgcg
     1681 aaggactttc ttcacatttt gtggggtatt ttgtagaatc ttgacatcca catagaagac
     1741 aaaatgccat caagatataa actacatttt ttactatcct atactttctc atactacact
     1801 tataatacac ctatttctta tgaatcaaat cattaaatgc atcaatcgac aaaggacgac
     1861 tataataata tccttgtccc acattggcac ctagacatct tataaattca tcatccttgc
     1921 ttgattcaac accttcaacg acaacacgta tatttgattt tttcgcaata ttgataattc
     1981 cttctaaaat ccattttgtt tttttgtttt ctaagtccga atctatatgc aagaagatac
     2041 gatcaatttt taattcatca atagtaagtt tcgataaaac atttaaagat gaatatccac
     2101 taccaaaatc atctagagat acagaaaaac ctaatttttt tacttccaaa agaatttgat
     2161 taaacaattt aaaatcttca ataaccgtac tctctaagac ctctaaagtg atataggaag
     2221 ccgaaacatc gtactccttt aacaaggact ttaattctga aatataattt tcatgataaa
     2281 aaacaatttt tgattgattc actgaaagat tgactggttg aattcccata tcgatccatt
     2341 tacgtatttg ttgaatagct tttcttaaca tgtaaaagtc tagttttgaa caaaaccgat
     2401 tcttttcaaa aatccctaca aaacttgatg gaggaagata ggttccatcc tctaatttcc
     2461 atcgaaccaa ggcttcggca gaaacaacta aattcgtatc taaatcaacc ttaggctgta
     2521 ataccatttc aaaacgattt gaatctaaag cttgttgcat attggattcc acaaaatttt
     2581 caaactcttt tttcttatgc acttcttcat caaaaaaaca aatattttca gaagccattt
     2641 ccttcgactg agctaaggca aacataacat ttgtaagcat ttgttcaaat gagtattttt
     2701 cttgccttgc accattacaa ataacaccac aacaaaattc gtattcataa caaaaatctt
     2761 ttgtattttg acgaatacta ttaaatatcg catgaatacg atttgaaacc ttgttttcag
     2821 ccgtatccat caataacata taaaaaacat cggcactgtc tctacaaata atttcatcct
     2881 ttttcagcat tggctttata caagttacaa tacgacacaa aaaatcatct gacttatctc
     2941 ttcttaaaat ctcattcata aatttaaatc gacgcatatt taaagatacc aatgaataat
     3001 tattattttt aagggcatat atacgaacca actgcgtaaa tcgagcaaaa gaaacggcac
     3061 cagttaattg atctgtataa gctaactttt ccatctcttc attgtttttt accatcattc
     3121 tataagcgat ccacaataaa agaataacta caatcacaaa taacatcaaa acataattcg
     3181 tagtacgcga catcacatat aaaggataat ttaatgattt agctgaattt acataacata
     3241 aataccaacc ttgaatccca atttttttta caacaattcc atgttcttga cctttgactc
     3301 ctatcaatcc attcacattt tcggatagat tcatattttt tatgacagct aacacttcat
     3361 ttgattctaa attcttttga attccataaa aatctttaaa atccgtaatg tacaaattcc
     3421 caccacttat tgattttctc atcaaatctg tatatggttt caagctacta tcagcacaca
     3481 atactccaac aatctgattt tcattcccat gaacaggcac tatatatgta agcttataat
     3541 tcttattttt ttgtttaaat gtactttgct cccccaaaaa accattctta attttatctt
     3601 tatattcatc tgataaatta ttatacgaaa acttagaatc cttttttgaa gaatacacat
     3661 tatgattcat atcgataaat tgaatcgatg aaaaatcact ctcttttttc atttcaaata
     3721 atgaggaatc taaatctggt tccttattta aatttcttgc gaaagtattc a
//
LOCUS       DBMDWL010000185         3765 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_10678_length_3765_cov_3.766648, whole genome shotgun sequence.
ACCESSION   DBMDWL010000185 DBMDWL010000000
VERSION     DBMDWL010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10678_length_3765_cov_3.766648"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            96..1442
                     /locus_tag="ACZPDN_10280"
     CDS             96..1442
                     /locus_tag="ACZPDN_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1299047.1"
                     /translation="MKVKDMTKGNELKLIILFSLPLMLGNIFQQLYTVCDTIIVSRHL
                     GVKALAAIGCCDWLTWMGIAVVTGLAQGFSITVSHAYGTHDKSNVQKCISALVVNAII
                     STVVLLAIIYPLLPRILVLLKTPVDIMDRALAYTHVIYIGLFATMFYNALASILRAFG
                     NSKTPLHAMIIASICNVGLDVLFVMGLEWGIIGAGVATVIAQILAGCFCLYHVLKLKE
                     YMPNSICRDLSYYMNQFRLGIPMALQNVLIAIGGMVLTSAVNRYGTYFLAGFTAMNKL
                     YGVLEISAVSYGYAMVTYTGQNYGAKRYDRIKSGVKKVVLLSLATSILISIILWFFGP
                     FFLSCFISNTSQGAKEAMTYGLTFLRCLTIFLPILYMLHAYRSTIQGLSNAFIPMVSG
                     LFELMMRISGALVLPIFFGKSGLYPVEVLAWVGAVVILIPSYYHMERNFQNVSENS"
     gene            1556..3241
                     /locus_tag="ACZPDN_10285"
     CDS             1556..3241
                     /locus_tag="ACZPDN_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118114685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR1299048.1"
                     /translation="MEKILTEVFGIWYLIGAAFVFFMQAGFAMVEAGFTRAKNAGNII
                     MKNLMDFCLGTVAFLIIGYSLLMGHDFAGLIGWGESPLTDFAGTNWSSFTFNLVFCAT
                     AATIVSGAMAERTKFISYCIYSFVISLIVYPIEAHWVWGGGWLSSLGFHDFAGSAAIH
                     YVGGLTALIGAWMLGPRIGKFDKDGTPRGIPGHSLTIGALGVFILWFGWYGFNGAAAT
                     NGIQLATIFATTTVAPAIATCTVMLITWIKYGKPDVAMCLNGSLAGLVAITAGCDAVN
                     VFGSFVIGILSGCMVCFIVWLLDYKLKVDDPVGAVAVHFGNGVLGTICVGLFACGTDT
                     MPKAQGLFYGGGFHLLGVQLLGLLAIGAWTAVTMCITFFIIKKTVGLRVSAHEEIVGL
                     DKMEHGLESAYAGFALEADVPGDYLETVQDSSYTPSVDVEDAVPTKVIHSDGSISKVV
                     IITKAEKFDKIKAALNEIGIGGMTVTKVSGCGVQKGQTSYYRGAKVNMELLPKLKVEV
                     VVSEVPVSDVVKAAKKALYTGNIGDGKIFVYDVVDVVRISTSACGKDALKYEE"
     gene            complement(3326..>3765)
                     /locus_tag="ACZPDN_10290"
     CDS             complement(3326..>3765)
                     /locus_tag="ACZPDN_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299049.1"
                     /translation="CYYDFMINCEGIDYSPEEMQMCSIADLCLEKNDMFLLSFFNSET
                     DEFFPVTGKLINEELNNKEIELERIHVLEAKNDGPWVDENEHRTLEEQNDQLVSGFFF
                     NKMFYERPDLFEELENGKDIEELLFEMIDEELNDDVFDTDLLN"
BASE COUNT         1082 a          526 c          815 g         1342 t
ORIGIN      
        1 gccgtatgcg ggaaaactgc acgtacggtt cttagaggag tttatctcca actgtttaaa
       61 gtaaatatga gtaaaagaag gaggtaaatt tacttatgaa agtaaaagat atgacgaagg
      121 gaaatgaatt aaaactaatt attttgtttt ctctaccttt aatgttagga aatatatttc
      181 aacaactcta tacagtgtgc gatacaatca ttgtttctag acatttaggc gtcaaggcgt
      241 tagcggctat tggatgttgt gattggctta cttggatggg aatagctgta gtaactgggc
      301 ttgcccaagg tttttcaatt actgtatctc atgcatatgg tacacatgat aagagtaatg
      361 ttcaaaagtg tatttctgct ttagttgtta atgcgatcat atcgactgta gttttattag
      421 ctattattta tccattatta ccaagaattc ttgtgctatt aaagacgccg gttgacatta
      481 tggatcgtgc attggcttat acacacgtaa tttatattgg tttatttgcg actatgttct
      541 acaatgcgtt ggcaagtatt ttacgtgctt ttggaaattc aaaaacacca ttgcatgcca
      601 tgatcattgc cagtatttgt aatgttggtt tagatgtatt gtttgtcatg ggacttgagt
      661 ggggcattat tggagcaggt gttgcaacag tgattgcaca aattctagct ggatgtttct
      721 gtttatatca tgtattaaag ttaaaggaat atatgccaaa ttcaatttgt agagatttgt
      781 cgtattatat gaaccagttt agattgggta ttcctatggc tttacagaat gttttaattg
      841 cgattggtgg catggtttta acaagtgctg taaatcgata tggaacatat ttccttgcag
      901 gttttacggc tatgaataaa ttatatggag tgcttgaaat ttcggctgtt tcttatggat
      961 atgccatggt tacatatacg ggacaaaatt atggtgcaaa aagatatgat cgtattaaga
     1021 gcggtgtaaa aaaagttgtg ttactatctt tagccacttc cattttgatt tctataatac
     1081 tatggttctt tggaccgttc ttcctttctt gttttatttc aaatacaagc caaggagcta
     1141 aagaggcgat gacatacgga ttaactttct taagatgttt aaccattttc cttccaattt
     1201 tatatatgtt gcacgcatat cgctctacaa ttcaaggttt atcgaatgct tttattccga
     1261 tggtgtcggg attatttgaa ttaatgatgc gaatttcagg cgctttagta ttacctatct
     1321 tttttggtaa atctggatta tatccggtag aagtattggc gtgggttggt gctgttgtca
     1381 ttttgattcc gagttattat catatggaaa gaaattttca aaatgtttca gaaaatagtt
     1441 gacaaactgt ttcatagaga ataaaatacg tacatacata aggcaaaggt gcttatagtt
     1501 catgaaatat atggactgta agtgcctttt tcttttgtgt aagggaggaa atattatgga
     1561 gaagattttg acagaggtat ttggaatatg gtacctgatc ggagctgcct ttgtattctt
     1621 tatgcaggca ggatttgcga tggtagaagc cggatttaca agagccaaga atgccggtaa
     1681 catcattatg aaaaacttga tggatttctg tttaggaact gtcgcattct tgattattgg
     1741 ttatagtcta ttgatgggac atgattttgc aggattaatt ggttggggag aaagtccatt
     1801 gacagatttt gccggaacaa actggtcaag ttttacattc aacttagtat tctgtgcaac
     1861 agccgctacc attgtttctg gagctatggc tgaaagaact aaatttattt cttattgtat
     1921 atatagtttt gtgattagtt taattgtata tccaattgaa gcacactggg tatggggcgg
     1981 tggatggcta agtagccttg gcttccatga ttttgccggt agtgctgcca tacactatgt
     2041 tggtggttta acggctttaa ttggtgcatg gatgttaggt ccaagaattg gtaagtttga
     2101 taaagatgga acgcctcgag gaattccagg acacagctta acaattggtg cattaggagt
     2161 ctttatctta tggtttggct ggtatggatt caatggtgca gctgctacaa atgggattca
     2221 gttagctaca atttttgcga caactactgt tgctcctgct attgcgacat gtacagttat
     2281 gttgattact tggatcaaat atggaaaacc ggatgttgcg atgtgtttaa atggatcatt
     2341 ggcaggttta gttgcgatta ctgctggatg tgatgccgta aacgtatttg gatcatttgt
     2401 gattggtatc ttatctggat gtatggtttg tttcattgta tggttattgg attataaatt
     2461 gaaagtagat gatccagtag gagctgttgc cgtacacttt gggaatggtg tattaggaac
     2521 aatctgtgtt ggattatttg cgtgtggtac cgatacaatg cctaaagcac aaggattatt
     2581 ctatggtgga ggattccatt tattaggtgt tcaattatta ggtttattgg caattggtgc
     2641 atggactgca gtaacgatgt gtattacttt ctttattata aaaaaaacag ttggattacg
     2701 tgtaagtgca catgaagaaa ttgttggtct tgataaaatg gagcatggat tggaaagtgc
     2761 atatgctggt tttgccttag aagccgatgt tcctggtgat tatttagaaa ctgtacaaga
     2821 ttctagttac actccaagtg tggatgtgga ggatgctgta cctacaaaag tcattcatag
     2881 tgatggttct atttctaagg ttgtcatcat tactaaagct gagaaatttg ataagatcaa
     2941 ggctgcatta aatgaaattg gcattggtgg tatgacggtt acgaaagtat ctggttgtgg
     3001 tgtacaaaag ggtcagacaa gttactatcg tggtgcaaaa gtaaatatgg aattgcttcc
     3061 taaattaaaa gttgaagtcg ttgtaagtga agtgcctgta tctgatgtag taaaagcagc
     3121 taaaaaggct ttatataccg gtaatattgg ggatggaaaa atctttgtct acgatgttgt
     3181 tgatgttgtt cgtatttcaa catctgcttg tggtaaagat gcattaaaat atgaagaata
     3241 atatatttcg tggcttgtaa aattgctctt ttctcttatt cattacaagc cataagaaaa
     3301 ggatcgaatt taatttcgat ccttttcagt ttaataaatc tgtatcaaat acatcatcat
     3361 ttaattcttc atcaatcatt tcaaaaagca gttcttcaat gtctttaccg ttttctaatt
     3421 cttcaaatag atcgggtctt tcatagaaca tcttattaaa gaagaatcca gaaactaatt
     3481 gatcattttg ttcttctaaa gtgcgatgtt cattttcatc tacccaaggg ccatcgtttt
     3541 tagcttcaag tacatgaatg cgctctaatt cgatttcttt attatttagt tcttcattga
     3601 tgagttttcc tgtcactgga aagaattcat ccgtttcaga attgaagaag gatagtaaga
     3661 acatgtcatt tttttctaaa cataaatcgg caatggaaca catttgcatt tcttctggtg
     3721 aataatcaat gccttcacaa ttgatcataa agtcatagta acatg
//
LOCUS       DBMDWL010000186         3726 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_10838_length_3726_cov_2.080000, whole genome shotgun sequence.
ACCESSION   DBMDWL010000186 DBMDWL010000000
VERSION     DBMDWL010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10838_length_3726_cov_2.080000"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="ACZPDN_10295"
     CDS             <1..1028
                     /locus_tag="ACZPDN_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012234.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1299050.1"
                     /translation="TLNLMPIVKALYAQFVYELGRYYLFNKNDKKDIVKDHFIQYIKL
                     LDIKQEKKAIVVDEPKEESSDSESLEDLQKQLDSMIGLDGVKQQVHSIINELKIDELR
                     KSKGLKVSSTSKHLVFKGNPGTGKTTIARLLSKIYKELGILEKGQLVEVDRGGIVAGY
                     VGQTALKTKEKIDEAMGGILFIDEAYTLAKGENDFGQEAIDTLLKAMEDHRDEFIVIV
                     AGYDEPMEAFLQSNPGLKSRFNEYIHFDDYSAEELFKIFGLLCEQNDFKMDLDAQETL
                     KSYLNELCIHKPDNFANGREMRNLFDKSKSAHANRLASLNEISDKDLITFKKDDVLKA
                     IEEMKLI"
     gene            1176..1487
                     /locus_tag="ACZPDN_10300"
     CDS             1176..1487
                     /locus_tag="ACZPDN_10300"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1299051.1"
                     /translation="MLGAIIGDIVGSVYEFSNNKTKDFELFKPDCQFTDDTVMTCAVA
                     DAIMNGRSEKDFILSMKKYGRMYPHAGYGGNFRFWLRSKNTEPYNSFGNGSAMRVSPC
                     A"
     gene            1491..1763
                     /locus_tag="ACZPDN_10305"
     CDS             1491..1763
                     /locus_tag="ACZPDN_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299052.1"
                     /translation="MDCGFCARTGMWPNYDLARLSASVTHNHPEGVKGALAICDAIFM
                     ARFYSDDCKRRIKEHIEKEYGYDLSMSLDSIRKDYKFDETCQGCVP"
     gene            1770..1991
                     /locus_tag="ACZPDN_10310"
     CDS             1770..1991
                     /locus_tag="ACZPDN_10310"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1299053.1"
                     /translation="MAFLESTDFEDAIRNAISIGGDSDTIVAITGSIAEAAYGIPKEM
                     KEKALSYLDEPLKDVLRRWKKYMRGKNHG"
     gene            1984..2136
                     /locus_tag="ACZPDN_10315"
     CDS             1984..2136
                     /locus_tag="ACZPDN_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299054.1"
                     /translation="MDEVVDLLNEILDELRTMNEKLDDIRGNEYSDGSLEDIFDKLDE
                     INDNIG"
     gene            2251..>3726
                     /locus_tag="ACZPDN_10320"
     CDS             2251..>3726
                     /locus_tag="ACZPDN_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024865.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR1299055.1"
                     /translation="MQDFKLLTRQLLETYLGSDTLGEVAIQDFLDANASIIGTGKQEF
                     FKNIEHFLNSFKFDVEKRKNIHFEFKNLEIEENIVDDQCVFVYGSVQIYGLYDKEVTI
                     INLDSRFTIIYGVRDGKWKVLHIHHSIPDKEQLEDEEFPITLGKQVQQARHEVEALSA
                     GYSYICLINLETGDVELIKGNNIPGLNGRYTQTDHNILLEQVNRLIAEPFRKNSVEFY
                     DSKTIAKRLEGKNSLFHTCERTDGTWILSMIVPQKYDVNGRVVSVLLANRDFTEEKKR
                     ELEQEEALRQEKIKAEKANEAKSIFLFNMSHDIRTPMNAIVGYSQLMKKELTNPKLIH
                     YQEMIEQSSKLLLSIINNVLDMARVESGKMELDENYEVIGNISQLVCSAFAAEASRKN
                     IELNIIVNVEHKHIIVDSTKMQEILSNLISNAIKYTSAGGKVTIDTKELPYDKEGYVL
                     IQTKVSDTGIGMSEDFLPSLFELFTRERNTTLSKIPGTGLGM"
BASE COUNT         1302 a          429 c          793 g         1202 t
ORIGIN      
        1 aaacattaaa tttaatgcct attgtaaaag ctttgtatgc acaatttgtg tatgaactgg
       61 gtcgttatta tttgtttaat aagaatgata aaaaagatat tgtaaaggat cattttattc
      121 aatacattaa attattggat attaaacaag agaaaaaggc aatagttgtg gatgagccta
      181 aagaagaatc tagtgatagt gagtctttag aagatctgca aaaacaattg gattctatga
      241 ttggattgga tggtgtaaaa caacaagtcc attcaattat taatgagttg aaaatagatg
      301 agcttcgtaa aagtaaaggc ttgaaagttt ccagtacttc taaacatctt gtgtttaaag
      361 gaaatcctgg tacgggaaag acaacgattg ccagattact atctaaaatc tataaagagc
      421 ttggcatatt ggaaaaaggc caattggttg aagtggatcg tggtggaatt gtagcgggat
      481 atgttggtca aacggcttta aagactaagg aaaagattga tgaggctatg ggtggaattc
      541 tctttattga tgaagcctat actttagcta agggtgagaa tgattttggt caagaagcca
      601 ttgatacttt attaaaagcg atggaagatc atcgtgatga gtttattgtg attgtagctg
      661 gatatgatga gccaatggaa gcattcttac aatcgaatcc tggactaaaa tcgagattca
      721 atgaatatat tcattttgat gattattctg cagaagaatt atttaagatt tttggattat
      781 tgtgcgaaca aaatgatttt aaaatggatt tagatgccca agaaacttta aagtcctatt
      841 tgaatgaact atgtattcat aaaccggata attttgcgaa tggacgagaa atgcgtaact
      901 tgtttgataa gagtaagagt gctcatgcga atcgtttggc tagtttgaat gaaatatccg
      961 ataaggattt aattacattt aaaaaagatg atgtattaaa agcaatcgaa gaaatgaagt
     1021 taatataaaa agaaaaaact aaccccagga gcaaaggcat ctcgatctac atcttactgg
     1081 cacagccagc ccagctttat ccatcacaag ttagtttctg tatgtatgat actatatatt
     1141 tttaaaaaaa tccataatag aaaaaagatg atactatgtt aggagctatt attggtgata
     1201 ttgtaggttc agtttatgaa ttttcgaata acaagacaaa agattttgaa ttgtttaaac
     1261 cagattgtca atttacagat gataccgtta tgacttgtgc tgtggcagat gcgattatga
     1321 atggtagaag tgaaaaagac tttattttat ctatgaaaaa gtatggtcgt atgtaccctc
     1381 atgctggata tggtggtaac tttcgttttt ggttaagaag taagaatact gagccatata
     1441 atagttttgg caatggttcg gccatgcgtg tatctccttg tgcttgagtg atggattgtg
     1501 gtttttgtgc aagaacaggt atgtggccca attatgactt agcaagatta tcggctagtg
     1561 taacacacaa tcatccagaa ggtgttaagg gtgctttagc tatatgtgat gcgatcttta
     1621 tggctaggtt ttattcggat gattgtaaaa gaagaatcaa agaacatatt gaaaaagaat
     1681 atggctatga tttatcaatg tctttagatt caattcgtaa ggattacaag tttgatgaga
     1741 cttgtcaagg ctgtgttcca taggccatta ttgcattttt agaaagcacg gattttgagg
     1801 atgcgattcg aaatgcgatt tcgattggtg gagatagtga taccattgtg gcaattacag
     1861 gaagtattgc agaagcagcg tatggtattc ctaaagagat gaaagaaaag gctttatctt
     1921 atttggatga gcctttaaaa gatgtattaa gaagatggaa gaagtatatg aggggtaaaa
     1981 atcatggatg aagtagtgga tttattaaat gaaattctag atgaattaag aacaatgaat
     2041 gagaaattgg atgatattcg tggaaatgaa tatagtgatg gttctttaga agatatattt
     2101 gataaattag atgaaattaa tgataacatt ggttaaaatt aatggtatga tataagcaat
     2161 gatatatctt ttcaaatcag ttagaaataa taatataatg tggcattgtc tgatttgttt
     2221 gatgatgcct tttgtttgtg gggttgggtt atgcaggatt ttaagttact tactagacaa
     2281 ttgttagaaa catatctagg atcggataca ttgggcgaag ttgcgattca ggatttttta
     2341 gatgcaaatg ctagtattat tggtacggga aaacaagaat tctttaaaaa tattgagcac
     2401 tttttaaatt ctttcaaatt tgatgtggag aaaagaaaga atattcattt tgaatttaaa
     2461 aatttagaaa ttgaagaaaa tattgtggat gatcaatgtg tttttgtgta tggatctgtt
     2521 caaatttatg gtctttatga taaagaagtt acaattataa atttggattc tcgctttact
     2581 attatttatg gagttaggga tggaaaatgg aaggtgcttc atattcatca ttcgattcca
     2641 gataaagaac aattggaaga tgaagagttt ccaattactt taggtaaaca ggtgcaacaa
     2701 gctcgacatg aagttgaggc attatcggct gggtattcct atatttgttt gattaatcta
     2761 gagacaggtg atgtggaatt aattaaagga aataatattc ctggcttaaa tggacgctat
     2821 actcagacgg atcataatat attattagag caagtaaatc gtttgattgc cgaaccattt
     2881 agaaagaata gtgttgaatt ttatgatagt aagaccatcg caaaacgtct tgaaggaaag
     2941 aattccttat tccatacttg tgaaaggaca gatggaactt ggattctttc gatgattgta
     3001 cctcaaaaat atgatgtgaa cggtcgtgtt gtgagtgtat tgcttgcaaa tcgtgatttt
     3061 acggaagaaa agaagcgtga gttagaacaa gaggaggctt tacgccaaga aaagatcaag
     3121 gctgaaaagg ccaatgaagc caaatccata ttcttattta atatgtcaca tgatattcgt
     3181 acaccgatga atgcgattgt gggatattca caattaatga agaaagaatt gacaaatcca
     3241 aagctgattc attatcaaga aatgattgaa caatccagca aattattact ttcaattatt
     3301 aataatgtat tggatatggc tcgtgttgaa agtggtaaga tggaattgga tgaaaattat
     3361 gaagtcattg ggaatattag tcaacttgtt tgtagtgcat ttgcagcaga ggcttcaaga
     3421 aagaatattg aattaaacat aattgtgaat gtcgaacaca aacatattat tgtcgattct
     3481 acaaagatgc aggaaattct atctaaccta atcagtaatg cgataaaata tacatctgct
     3541 ggtggtaagg taacaatcga tacaaaagaa cttccttatg ataaagaagg ctatgtattg
     3601 attcaaacaa aagtatcgga tacaggtata ggtatgagtg aagatttctt gccatcttta
     3661 tttgaattat ttacacgtga aagaaataca actctatcta aaattccagg tactggactt
     3721 ggtatg
//
LOCUS       DBMDWL010000187         3652 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_11169_length_3652_cov_4.390876, whole genome shotgun sequence.
ACCESSION   DBMDWL010000187 DBMDWL010000000
VERSION     DBMDWL010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11169_length_3652_cov_4.390876"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(803..874)
                     /locus_tag="ACZPDN_10325"
     tRNA            complement(803..874)
                     /locus_tag="ACZPDN_10325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Gln,seq:ttg)
     gene            complement(884..1258)
                     /locus_tag="ACZPDN_10330"
     CDS             complement(884..1258)
                     /locus_tag="ACZPDN_10330"
                     /inference="COORDINATES: protein motif:HMM:NF023181.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HIR1299056.1"
                     /translation="MNNIQISGYCVDCINQFALFSAEPCKSCINRGGKKDNFAPLKDF
                     DFAPSVNEKPVNDNVNHPSHYATGKYECIDVMLEIFGVEAVKTFCLLNAFKYNYRTGN
                     KNGLEDIKKAKWYIDKYIELSE"
     gene            complement(1258..1659)
                     /locus_tag="ACZPDN_10335"
     CDS             complement(1258..1659)
                     /locus_tag="ACZPDN_10335"
                     /inference="COORDINATES: protein motif:HMM:NF015758.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HIR1299057.1"
                     /translation="MQGAEYQALAMRTNNKKSTDRLLNKINNLKIGNRGEDTPEIELG
                     GVLNAALGLSGEVGELNDMLKKWIFHEKQLDSEHLKREISDVCWYLALMCDSFEFNLD
                     EIMQINIDKLKARYPEGFDTYRANHRQAGDV"
     gene            complement(2202..2912)
                     /locus_tag="ACZPDN_10340"
     CDS             complement(2202..2912)
                     /locus_tag="ACZPDN_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299058.1"
                     /translation="MEKVQEAVESQEIFENEIEAHFKRFCEDENIEDMAAAPQSLFYA
                     ALIYVYNNTFKGTNRLKLKGKLQGYNNNNYNNQYSNINNSNCNSYNYEYLNYIADYYI
                     YMCYKYNKICTISGYCKLTGINETVIYDWANEKRASKLSTSAYDLWEKLSKDYESSGE
                     ARLWSGKNPVGQLAVMNRRFGWNLPGVSRESTAKVIKTAADLPRLGTSGNAQGSNVRQ
                     IAQQEIIVQDVQENPQSQ"
     gene            complement(3116..3346)
                     /locus_tag="ACZPDN_10345"
     CDS             complement(3116..3346)
                     /locus_tag="ACZPDN_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299059.1"
                     /translation="MKEFKMYNGNMLEVGEGIRLADLWQSDDGDEEEILDSGCCWVGD
                     DENDMPITADFEILEKDEEDLLNSLVKVTDIR"
BASE COUNT         1092 a          777 c          544 g         1239 t
ORIGIN      
        1 cttgtgctta atctgtgaat aacaaaaaca acaaacaggc agttcacagg tctgcttttc
       61 gcttcgtcaa gctagtttgt tgtaatcgga tagacagggc tcgaacctgt gactacttga
      121 ataaatcaag cgttactccc atctgaacta ctatccgttg tacagtttct tgtgttgaaa
      181 gtatttatgg cacttcatta cactatctgc catcctgttc gcaaatcaac caacacaagc
      241 attttaatta ttcagcaggg aatactgcaa cgcctgctta ttcgggagct acccgaaaac
      301 ttgctatggt gaggatttgc acctccacat gacacttaag acgagttatc taagttgcag
      361 atttcaactc ataaatctac tgcaatactg gctacctatt tcagcacata gcaacttact
      421 cacacctctt aacctaggat aagtccgcaa acagcattac gcacgcagac ctaagaagtg
      481 ctttcaaaac gccgacatcg tgaatcgaac acgaacaaca tttctgctgg atagcttagc
      541 aagctactgg aataccttta tcccatatcg gcaaataccg cctgtaacgg ctatcaagaa
      601 acaagaacag aaacaataaa atattagggg tattttagta aggagtgctt cttgataagt
      661 tggttttcac acctctgtgc gaggtaaaac tctccgagtg gtcttgcacc acccttaact
      721 gaaacaaatc caagagagca tatgaaggag gactaccctg taaaatgcaa aacatggtag
      781 tctacgataa aagtaagaca aactacacca gtcggattcg aaccgacgca tacagaggtc
      841 aaagctctgt gccttaccgc ttggctatgg tgcattgatg tgattattct gacaattcta
      901 tgtacttgtc aatgtaccac ttggcttttt taatatcctc taagccattc ttgttgccag
      961 tgcggtagtt atacttaaaa gcattaagca agcaaaatgt ctttacagct tcaacaccaa
     1021 atatctcaag cataacatct atgcactcat atttaccggt tgcataatgg ctaggatgat
     1081 taacattgtc atttaccggc ttttcattaa cactaggtgc aaaatcaaaa tctttgagcg
     1141 gagcaaagtt atctttcttg ccgccgcgat taatacaact cttacacggt tctgcactga
     1201 ataatgcaaa ttggtttatg caatcaacgc aatatccact aatttgaatg ttattcatta
     1261 aacatcacct gcctgtctat gattagctct gtaggtatca aatccctctg gatatcttgc
     1321 tttcagctta tcaatgttaa tctgcatgat ttcatcaagg ttaaactcaa aagaatcaca
     1381 cattaaagct aagtaccaac atacatcgct gatttcacgc tttaaatgtt cagaatctaa
     1441 ctgcttttca tggaaaatcc atttcttaag catgtcgtta agttctccaa cctcgccgga
     1501 taaacctaat gcagcattaa gaacaccacc taattcaatc tctggcgtat cttcaccgcg
     1561 attaccaatc tttaagttat taatcttatt cagaagccta tctgtagact ttttattgtt
     1621 agtacgcata gccaaagcct gatactctgc tccctgcatt tctaactcct aactcttttt
     1681 tattttttaa aattttttgg aatttactcg gctgaattag ccgttttgat gtgtgtattt
     1741 attgaatatc ttgtgaataa ttaagatgtg tctattatac acctatctat cagatttgta
     1801 cagtagattt attgattata ttatatgggt tattatcagg actatatatt aataaatata
     1861 atggttattg tatatagttt aataaattat tattggttgg ttatgtatat ataaatatat
     1921 ataataagcc tttttatctt tggggttgga aaagcgactt agttgggcgt gcaatgcgtg
     1981 tatatataac ccccacgccc tgcgtttgtg tatcttgcac aacgaaatca gccagagcgg
     2041 agccattgcg cgatgaataa ttatcacgca atcgctgtta atccgcttgt tcactagctt
     2101 tgtcgtgctt ttatcgttca aatgttctgt tttatcactt cgctaaactc aactttagcg
     2161 aaatcattac ataatatgca aaagtgccac aaaccgcttg tttactggct ttgtgggttt
     2221 tcttgtacat cttgcacaat aatttcctgt tgtgcaattt gacgaacatt agaaccttgg
     2281 gcatttccag atgtgccaag ccgcggaagg tctgcggctg ttttaatgac ctttgcagtg
     2341 ctttctctgc tcacacctgg aagattccaa ccaaaacgac gattcatgac cgcaagctgt
     2401 ccgactgggt tcttgccaga ccagagccgt gcttccccac tagattcata atcttttgac
     2461 agtttttccc acaaatcgta agccgaagta cttagttttg acgctctctt ctcattagcc
     2521 caatcatata taacagtttc atttatacca gttaatttac aatatcctga tatagtacat
     2581 attttattat acttataaca catatatata taatagtctg ctatataatt taagtactca
     2641 taattataac tattacaatt actattattt atattactat attgattatt ataattatta
     2701 ttattatatc cctgtaattt accctttaat tttagtctgt tagtgccctt aaaagtatta
     2761 ttatatacat aaattaaagc ggcataaaaa agggattgcg gagccgctgc catatcttca
     2821 atgttttcat cttcgcagaa tcttttgaaa tgtgcttcaa tctcattttc aaaaatttct
     2881 tgactttcta ctgcttcctg tactttctcc atgtgttccc ctttctgctg gatctgctcc
     2941 agctaattaa ttattatata tactaataac ataaaaataa cccgataact attatataac
     3001 tatcgggtgt aaatcttata tatttaatta ttagcaatat aataacacaa taaatataat
     3061 taatcaatag gcattaaaaa agcgatgtat aacaatatac accgcctaaa tcatactatc
     3121 ttatatctgt aacttttaca agagaattca gcaaatcttc ttcatctttc tccaagattt
     3181 caaaatctgc agttattggc atgtcattct catcgtcgcc aacccagcaa caaccagaat
     3241 caaggatttc ttcttcgtct ccgtcgtcac tctgccataa atcagcaagt ctgattccct
     3301 cgccaacctc taacatatta ccattgtaca ttttaaactc tttcatatcg tccacctttt
     3361 aacctttctt aattgttttc tttttcgcat tcaaacccga ataaaatatc attcgccaac
     3421 tcttcgctta tttcttcctc tgcgattggc tttctgttct ctgctccaat cacttcatca
     3481 aggcttatat ctatgtctgc aagtgccttt ttctgctaaa tccaagctta ataactttgt
     3541 ttaataattc aattgttttc atcctttcca ccttttcagc ctttcggatg ccctttctta
     3601 atttgtataa ttataatatc ataagcaata cataaatgca atacataatt ga
//
LOCUS       DBMDWL010000188         3646 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_11201_length_3646_cov_1.753738, whole genome shotgun sequence.
ACCESSION   DBMDWL010000188 DBMDWL010000000
VERSION     DBMDWL010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11201_length_3646_cov_1.753738"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="ACZPDN_10350"
     CDS             <1..255
                     /locus_tag="ACZPDN_10350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1299060.1"
                     /translation="PNDKMIQLAKNLSNTMNAINLSQFEGIDSDHYENFPILYDSQIP
                     AILLELGYLSNSDDYTKLVDETFQQKIADAITESFLAKIN"
     gene            309..821
                     /locus_tag="ACZPDN_10355"
     CDS             309..821
                     /locus_tag="ACZPDN_10355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1299061.1"
                     /translation="MEEKLIYKGIIFDLVQKDVKIKDKMYKRDIIRHPGGVGVICIID
                     GKILLVRQERPAIGKETLEIPAGKLEYGEDPMECGLRELNEETGMACDKLELLLSFVS
                     TPGFCDERIWIYKAIHPRMADFKLSLDEDEEIDTLWLDLETAYEYTRNGKIDDGKTVI
                     AISQMMAERK"
     gene            826..2022
                     /locus_tag="ACZPDN_10360"
     CDS             826..2022
                     /locus_tag="ACZPDN_10360"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR1299062.1"
                     /translation="MNFVVISPNYPESYWMFCRGLKENGAKVLAIVDTPYNQLKQELK
                     EYCDEIYVVKSFHDYDEMIKACGYFTFKYGKIDWIESNNEAWLDLDARLRDDFNVKTG
                     FSLERIEELQSKSQMKKYYKEANVPVADYKVLHTLQDGLDFADKVGYPLVMKPDHGVG
                     ASMTYKIMNAEQLESVYMQTKDHVMILEQYIDGDVFTLDGICDENGDIRYLNSLEYVG
                     NCMDSVLYQQSIGCFTTLEISDECRDIVQRTIHAFKIKNRFFHCEFFRLNHDVQGLGE
                     KGRIFGLEVNFRPPGGFCPDLMNYAGELDVYRLWAEVILKQQASYSKLRRYSAGFVGR
                     RNSIKYRFTVKEIQDMFKEELIDILYLPEAIAAAMGDVAIVAKFTSPSRRDEFFKVAL
                     KRKDRL"
     gene            2043..2774
                     /locus_tag="ACZPDN_10365"
     CDS             2043..2774
                     /locus_tag="ACZPDN_10365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1299063.1"
                     /translation="MSKLNEKRKIFIYLPKDYDMSNKQYPVLYAFDGHNIFLDAYATY
                     GMAIHIQEHIEKLNLDLIVVGQECSHIGNNRLKEYAPYPFYDPEYGSFEGLGDATMDF
                     FVNELKPYIDQNYRTKKTRKNTFIGGSSCGGLMALYAAYTYSDIYSKAIVISPYVLES
                     MDAILKDIHHKNIQKNTSIYLSWGSNEASLMHEFVWQTKACTEIGNSLSKKGVKVLYN
                     VIPEGRHCEEDWEKECDTFLPFLVQ"
     gene            2771..>3646
                     /locus_tag="ACZPDN_10370"
     CDS             2771..>3646
                     /locus_tag="ACZPDN_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1299064.1"
                     /translation="MNRDVVISKLETKAKRKIVISDIHGNLDLYQALLHKIDYKPGVD
                     CLILLGDLIEKGKRNLDTLHYIMHQVQTEDVHCIMGNCDFIAKNVLYSYRLDFLKHVL
                     GFRSASLIHEMAKSLNIEINENSNMSQICQILRKHYLDELCFLNDLPHVLEDDEHIYV
                     HAGIESNENYGTDFKQVMTHAFFLETNQVFKKNIIVGHMPVTEYCSHIASFDPIYDVK
                     HNIYSIDGGNMVKFGGQLNALIFEKNIVKVERCDFLKERKVIQNVDYHTQIPFFITFN
                     HGNIQILERNSIQSKV"
BASE COUNT         1294 a          513 c          687 g         1152 t
ORIGIN      
        1 cccaacgata agatgattca attggctaaa aacctatcca atacaatgaa cgcaatcaac
       61 ctatcgcaat ttgaaggtat tgattccgac cattatgaaa acttccctat tttatatgat
      121 agtcaaattc cggctatcct tttagaactt ggatatttaa gtaatagtga tgactataca
      181 aaattagtgg atgaaacttt ccaacaaaaa atagcggatg ccataacgga aagcttttta
      241 gctaaaatca attaacactc ttaggagtgt ttttttgtgc tataatctta acgaaaaaga
      301 ggtattgtat ggaagaaaaa ttgatctata aaggtattat ctttgattta gttcaaaagg
      361 atgtaaaaat caaagataaa atgtataaac gagacatcat tcgtcatcca ggaggtgtgg
      421 gtgtcatttg tatcatcgat ggcaagatct tacttgtacg acaagagcgt ccggcaattg
      481 gtaaagaaac acttgaaatt cctgcaggta aactcgaata tggtgaagat cccatggaat
      541 gcggacttcg tgaactaaac gaagaaacag gcatggcctg tgataaatta gaactattat
      601 taagttttgt atcaacacca ggcttttgtg atgaaagaat ttggatctat aaagccatcc
      661 atccaagaat ggcagatttt aaactttctt tagatgaaga tgaggaaatc gatactctat
      721 ggttggattt agaaacggct tatgaatata caagaaatgg aaaaattgat gatggaaaga
      781 ctgtcattgc gattagtcaa atgatggcag aaaggaaata agattatgaa ttttgtggtg
      841 atttcaccaa actatccaga aagttactgg atgttttgta gggggttaaa agaaaatgga
      901 gctaaagtat tggctatcgt agatactcct tacaatcaat taaaacaaga attaaaagag
      961 tattgtgatg aaatctatgt tgtgaaaagc ttccatgact atgatgaaat gattaaggca
     1021 tgtggctatt ttacatttaa atatggaaaa atcgactgga ttgaatccaa taatgaagct
     1081 tggcttgatt tagatgcacg tcttcgtgat gattttaatg taaaaacagg gttttcatta
     1141 gaaagaatcg aagaacttca atcgaagtca caaatgaaaa aatattataa agaagccaac
     1201 gtcccagtag ctgattataa agtattacat acacttcagg atggcttaga tttcgcggat
     1261 aaagtgggat atccactcgt tatgaagcca gatcatggtg taggtgcaag tatgacttat
     1321 aaaatcatga atgccgaaca attagaatct gtttatatgc agacaaaaga tcatgttatg
     1381 attttagaac aatatattga tggagatgtt tttacattag atggtatttg cgatgaaaac
     1441 ggagatattc gctatctaaa tagtttagaa tatgttggta actgcatgga ttctgtgctt
     1501 tatcaacaaa gtattggatg cttcacaaca ctcgaaatca gtgatgaatg tcgtgatatt
     1561 gttcaaagaa cgattcatgc ctttaaaata aagaatcgtt tcttccattg tgaattcttc
     1621 cgattgaatc atgatgtaca aggattaggt gaaaaaggcc gtatctttgg tctagaagta
     1681 aacttcagac ctccaggagg attctgtcct gatttaatga attatgcagg tgaactcgat
     1741 gtataccgtt tatgggctga agtgatctta aaacaacaag catcttattc aaaacttaga
     1801 agatattctg caggttttgt gggcagaaga aattccatca aatatcgttt tacagtgaaa
     1861 gaaattcaag acatgtttaa agaagaatta attgatatct tatatcttcc agaagccatt
     1921 gcggctgcca tgggtgatgt tgcgattgtt gccaaattta caagtccttc aagacgagat
     1981 gagttcttca aggttgcctt aaaaagaaag gatcgattat gatagttgaa aaagaacttt
     2041 tcatgtcgaa attaaatgaa aagcgaaaaa tctttattta tttacctaaa gattatgata
     2101 tgagcaataa acaatatcct gttttatatg cgtttgatgg tcataatatc tttttagatg
     2161 cctatgccac atatggtatg gccattcata ttcaagagca tattgaaaag ctcaatttag
     2221 atttaattgt cgttggacaa gaatgtagcc atatcggtaa caatcgttta aaagaatatg
     2281 caccctaccc tttctatgat cctgaatacg gatcatttga aggtttaggt gatgctacaa
     2341 tggacttttt tgtgaatgaa ttaaaacctt atattgatca aaattacaga accaaaaaga
     2401 caagaaaaaa tacatttatc ggtggaagta gttgcggagg cctaatggcc ttatatgcag
     2461 cctatactta ttccgacatc tattcaaaag ccattgtgat atccccttat gtcttagaga
     2521 gtatggatgc gattttgaaa gatattcatc ataaaaacat acaaaaaaat acttcaatct
     2581 atttatcttg gggttcaaat gaagcaagct taatgcatga atttgtctgg caaactaagg
     2641 cttgtactga aattggtaat tctctatcaa aaaaaggagt caaagtctta tataatgtga
     2701 ttccagaagg aagacattgt gaggaagact gggaaaaaga atgtgataca ttccttccgt
     2761 ttttagtgca atgaaccggg atgtagtgat ttcaaagctt gaaacaaaag ccaagcgaaa
     2821 gattgtgatt agtgatattc atggaaattt agatctatat caagctctct tacacaagat
     2881 cgattataaa ccgggtgtag attgtttaat tcttttaggt gatttaattg aaaaaggaaa
     2941 aagaaattta gatacccttc attacattat gcatcaagtt caaacggaag atgtacattg
     3001 tattatgggc aattgtgatt ttattgccaa aaatgttctt tattcctatc gtttggattt
     3061 tttaaagcat gttcttggat ttagaagtgc aagtttgatt catgaaatgg ctaaaagtct
     3121 aaatattgaa atcaacgaaa atagtaatat gtcccaaatt tgtcaaatct tacgcaaaca
     3181 ttatttagat gaactttgtt tcttaaacga tttaccccat gtattagaag atgatgaaca
     3241 tatttatgtg catgcaggta ttgaaagtaa tgaaaattat ggaacggatt ttaaacaagt
     3301 catgacacat gccttctttt tagaaacaaa tcaagtattc aaaaagaata tcattgttgg
     3361 acatatgccg gtgacagaat attgtagtca tattgcctct tttgatccca tttatgatgt
     3421 aaaacataac atttatagta ttgatggtgg aaacatggtt aaatttggcg gccaattaaa
     3481 tgctcttata tttgaaaaga atattgttaa agttgaacga tgtgattttc ttaaagaaag
     3541 aaaagtcatt caaaatgtag attatcatac acaaattccc ttcttcataa cttttaatca
     3601 tggtaatatt caaattcttg aacgaaattc gatacaatcg aaagta
//
LOCUS       DBMDWL010000189         3615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_11358_length_3615_cov_1.785714, whole genome shotgun sequence.
ACCESSION   DBMDWL010000189 DBMDWL010000000
VERSION     DBMDWL010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11358_length_3615_cov_1.785714"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="ACZPDN_10375"
     CDS             <1..1458
                     /locus_tag="ACZPDN_10375"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1299065.1"
                     /translation="VTGRTLFGAAPVKGQELDDHYFGSLSERVKAYMEEVDEELWKLG
                     VYAKTEHKEVAPCQFELAPVFTSTNIANDQNQLTMEVLQKVASHHGLVCLLHEKPFDG
                     VNGSGKHNNWSFCTEEGENILEPGDSPKDNIRFLTVLAAIIKGVDEYQDLLRISVASA
                     GNDHRLGADEAPPAIISIYLGEELTAILEAIEAGNEYVDTIDRRLELGVSTLPPIAKD
                     STDRNRTSPFAFTGNKFEFRMLGSNLNISCPNTILNTIVAEELTQFADELECVKKEDM
                     TKALIALIQKTLKNHKRIIFSGNGYSEDWKKEAQKRGLLELKTTADALPHYTDPKNLQ
                     LFEKHNVYSASELHARESILLTNYYQVVQIEAKTMAYMLRKQILPAIFSYEAKLAQII
                     QTKKSSGIDAPLEVKILKNINTPLELVSNHLDTLETLIHKETTTEKQAACFAANELLP
                     LMETIRQLTDLIETNVSKEDWPLPDYNDILFKSML"
     gene            1556..2725
                     /locus_tag="ACZPDN_10380"
     CDS             1556..2725
                     /locus_tag="ACZPDN_10380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR1299066.1"
                     /translation="MTKLFVHAKLLIDGINDTPKLDQLISIEDDKIKAIEDYHEIHED
                     VIEVNTIMPGFFNCHVHILYPVGFDSKKEFSLIEKIFYAQKHCKDYLESGVTFIRVVG
                     TEENYDLQIKEAIQNDVIQGPHMYCAGKVICMTGGHGWQEGIEADGKDACLKAVRTQL
                     KSGVDLIKMMATGGVMTKGVEPGNAQFSVEEMKVMIEEAHKAGRKTATHAQGLQGIKN
                     ALYAGIDSIEHGCFLDEECLELMKKQNTFLVPTLCAPQCIIDKGVENGVAKYMVDKTL
                     KVKDAHVKSVKKAYEKGIQIALGTDAGTPFNYHNNTAYEMELLAKLNIPNMDILKIAT
                     INSARCVGVEKDYGSIEVGKQADLVCLEENPLENISNVRKINKVIQSGKIVVENN"
     gene            complement(2834..>3615)
                     /locus_tag="ACZPDN_10385"
     CDS             complement(2834..>3615)
                     /locus_tag="ACZPDN_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299067.1"
                     /translation="KDEFFNHLLNGTVNEEKMEWIPFFYQFSKPLSNRYHKAVVQIAL
                     PNYYGLSMDMYQKMKDQAHFNEKLRQLNEPQTNACIEQKDTRLFYKLYFSILDYVNSF
                     EQIIPNKKIDPNIYIEPDELVNLIEVFWKDKDRFIDEYIEKNPSNFTFRNLNIISDFR
                     YGMRKNFLLVAYEKNYTVLNDEGINYMVKGLNENLDQFIAPEKTPMLMQTAIMPFNGR
                     IIYDGFISTSNIRLAQDIISKAFEDYSYGQKIYSLLPENLN"
BASE COUNT         1281 a          591 c          638 g         1105 t
ORIGIN      
        1 gttacaggaa gaacactatt tggtgcggct ccagtaaaag gtcaagaatt agatgatcac
       61 tactttggaa gtttatccga acgtgtaaaa gcctatatgg aagaagtaga tgaagaactt
      121 tggaaattag gtgtatatgc caaaacagaa cataaagaag tggctccatg tcaatttgaa
      181 ttggcacctg tatttacatc aacaaatatt gcgaatgacc aaaaccaact tacaatggaa
      241 gttttacaaa aggtagcttc acatcatgga ttggtatgtt tattacatga aaagccattt
      301 gatggtgtca atggtagtgg taaacacaac aactggtctt tctgtacaga agagggcgaa
      361 aacattttag agcctggtga ttctccaaaa gacaatattc gtttcttaac agtacttgcg
      421 gccatcatta aaggcgtcga tgaatatcaa gacttattaa gaatctctgt tgccagtgct
      481 ggaaacgatc atcgtttagg tgcagatgaa gcaccaccag ctattatttc tatctattta
      541 ggtgaagaat taacagctat cttagaagct attgaagctg gaaatgaata tgtagataca
      601 atcgatcgta gattggaact tggtgtttct accctacctc caatcgcaaa agattctaca
      661 gaccgtaacc gtacttctcc atttgcgttt actggtaata aattcgaatt tagaatgctt
      721 ggatccaact taaacatttc ttgtccaaac acaattttaa atacaatcgt agctgaagaa
      781 ttaacgcagt ttgcggatga attggaatgt gttaaaaaag aagatatgac aaaagccttg
      841 attgcattaa ttcaaaaaac attaaagaat cacaaacgaa ttatcttctc tggaaatgga
      901 tattcagaag actggaagaa agaagcacaa aaacgtggtt tattagaact taaaacaact
      961 gcggatgcac ttcctcacta tacagatcca aagaatcttc aattgtttga aaaacacaat
     1021 gtatattctg caagtgaatt gcacgcacgt gaaagtatcc ttcttactaa ctattatcaa
     1081 gtggttcaaa tcgaagctaa gacgatggct tatatgttgc gcaaacaaat cttgcctgca
     1141 atctttagct atgaggctaa actagctcaa atcattcaaa ctaaaaaatc aagtggaatc
     1201 gatgcacctt tagaagtgaa gatcttaaaa aatatcaaca cacctttaga actcgtttca
     1261 aaccacttag atacattgga aacattgatt cacaaagaaa caacaacaga aaaacaagcc
     1321 gcttgttttg cggccaatga attacttcca ttaatggaaa caattcgtca attgacagat
     1381 ttgattgaaa ccaatgtttc gaaagaagac tggcctttac cagactataa tgacattcta
     1441 tttaagtcta tgctttaggg aatccaagga ttccctcttt ttttacattt attttctgta
     1501 aatacttgca taaatagtat tcaaggttca taatcactta aaaaggagtt tagttatgac
     1561 taaattattc gtacacgcaa aattattgat tgatggcata aatgatacgc ccaaactgga
     1621 tcaattaatt tcaattgaag atgataaaat caaagcgata gaagattatc atgaaataca
     1681 tgaagatgtc attgaagtaa atacaattat gccaggcttc tttaactgcc atgtccacat
     1741 cctttatcct gtcggattcg actcaaaaaa agaatttagc ttaatagaga agatattcta
     1801 tgcacaaaag cactgtaaag attatcttga atccggagta acatttattc gtgttgtagg
     1861 cacggaagaa aattatgatc tacaaattaa agaagccatt caaaatgatg tgattcaagg
     1921 acctcatatg tattgtgccg gtaaagtcat atgtatgaca ggcggtcatg gatggcaaga
     1981 aggtattgaa gcagatggta aagatgcttg tttaaaagca gttcgtacac aactaaaaag
     2041 tggtgtagac ctcattaaaa tgatggctac aggcggcgtt atgactaaag gtgttgagcc
     2101 gggtaatgcc caatttagtg tagaagaaat gaaggtcatg attgaagaag cgcataaagc
     2161 gggaagaaaa acggctacac atgcccaagg tttacaaggt attaaaaacg ccttatatgc
     2221 tggtattgat tccattgaac atggttgttt tttagatgag gaatgtcttg aacttatgaa
     2281 aaaacaaaat acattcttag tccctaccct ttgcgctcct caatgtatta ttgataaggg
     2341 tgtagaaaat ggagtggcca aatatatggt agataaaacc ttgaaggtta aggatgcaca
     2401 cgtcaaaagt gtaaaaaaag cttacgaaaa aggtattcag attgcactag gtacagatgc
     2461 cggtacacca tttaactacc acaacaatac agcttatgaa atggagcttt tggcaaaatt
     2521 aaacattcca aacatggata ttttaaaaat cgcaacaatc aattccgcaa gatgtgtagg
     2581 tgttgaaaag gactatggtt cgattgaagt aggaaaacaa gcagatttag tatgtcttga
     2641 agaaaatcca ttagagaata tttcaaatgt aagaaaaatc aataaagtca ttcaatctgg
     2701 taagattgtt gtcgaaaaca attaaagacg ggattatatc caattcccgt ctttttctat
     2761 atcgattcat ttatcgtgat cttatttatc tattttaaag ctacaatttg aaatgtttaa
     2821 acaacattct cttttaattt aaattttcgg gcaataatga ataaatcttt tgtccgtaac
     2881 tataatcttc aaatgccttt gatataatat cttgggctag acgtatattc gatgttgaaa
     2941 taaagccatc ataaataatg cgtccattaa atggcataat ggctgtctgc atcaacatag
     3001 gtgttttttc tggggcaatg aattgatcta agttttcatt taagcctttt accatataat
     3061 tgataccttc atcatttaat accgtataat tcttttcgta tgccaccaac aagaaatttt
     3121 tgcgcatacc atatctaaaa tccgaaatga tatttagatt gcggaatgta aaattagaag
     3181 gattcttttc gatatattca tcaataaaac gatccttatc tttccagaag acttcaatca
     3241 aattaacaag ttcatcgggt tcaatataaa tatttggatc aattttttta ttaggaataa
     3301 tttgttcgaa tgaattcaca taatccaaga tactaaaata tagtttatag aataaacgcg
     3361 tatctttttg ttcgatacaa gcatttgtct gaggctcatt taattggcgc aacttttcat
     3421 taaaatgcgc ctgatccttc atcttttgat acatatccat actcaaacca taatagtttg
     3481 gcaatgcgat ttgtacaaca gccttatgat agcgatttga aagtggtttc gaaaattgat
     3541 aaaagaatgg aatccattcc atcttttctt cattgactgt accatttaat agatgattaa
     3601 agaactcgtc tttaa
//
LOCUS       DBMDWL010000190         3605 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_11402_length_3605_cov_2.972317, whole genome shotgun sequence.
ACCESSION   DBMDWL010000190 DBMDWL010000000
VERSION     DBMDWL010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11402_length_3605_cov_2.972317"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="ACZPDN_10390"
     CDS             complement(<1..261)
                     /locus_tag="ACZPDN_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299068.1"
                     /translation="MRYTTTSQEIFETFCISKKHKKHLKIDEEKKTIYFNYPEKDVEI
                     NYEPVDILYENTFLIVAFKPPFLLVHDDGNNQDTLQARVNGYL"
     gene            353..721
                     /locus_tag="ACZPDN_10395"
     CDS             353..721
                     /locus_tag="ACZPDN_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HIR1299069.1"
                     /translation="MKIFKNLENGSVVEGLLMDVVPNGYRELKTGEVDAAQEKHVPQV
                     SVEDNKLKVVVGSVEHPMLEAHYITNIWVEYSDGTVDRAGLVPGMKPEYVFDPKGRTG
                     KVTVYEYCNLHGLWKNEIEL"
     gene            784..2004
                     /locus_tag="ACZPDN_10400"
     CDS             784..2004
                     /locus_tag="ACZPDN_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR1299070.1"
                     /translation="MYKEKLRESFKVYDEIVLKCFCGIFIGAIVAICEVVFGKGLEWI
                     LNFREHMGCVMLLGLPFAGLAIVFLFDHWGRISRKGMGLVFEVDQGKSDWIPLRMAPF
                     MVVSTWITHFFGGSAGREGVAMQIGATVSHYFGKYFRFKNSGVIFMVAGMAAGFAGLF
                     GTPITAIFFALEVLVAGTLKYRAMSCAIPASFTAAYVSSLFGLHKSTFKIGSVSDLDV
                     ELSIKLLVLGILFGLIGGLFAFSLKHTKAFVVNKIKDPYKRIFMMGILVAILLFVFGK
                     CRYSGVGENLIVGSFTSEKIYSYDWILKFVLTILTLSAGFQGGEVTPLFAIGSSLGVI
                     IAPVFGLNPLFVAALGYCSVFGAATNTFLAPIAIGMEVFGYQYFPFFFVVCAIAYIVN
                     QNESIYALQRQVRE"
     gene            2120..3259
                     /locus_tag="ACZPDN_10405"
     CDS             2120..3259
                     /locus_tag="ACZPDN_10405"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1299071.1"
                     /translation="MKQNNMLAMILAGGRGTRLLELTKKNAKPAVYFGGKYRIIDFPL
                     SNCANSDINVVGVLTQYESVELNAYAGAGQRWGLDARGSGVYVLPPREKDGTKFDVYQ
                     GTADAITQNIDFIDKFDPEYVLILSGDHIYKMDYADMLSCHKANNADCTIAVLPVPMK
                     EASRFGIMNTDDEGRIVEFEEKPEHPKSNLASMGIYIFNWKQLRKALILDMTAEGSHH
                     DFGKDIIPNFLNANKRLYAYKFEGYWKDVGTIDSLWEANMDLLNKNNELNLDDPNWKI
                     YTEDIPTLPHYVGPTGKVEHCYINQGAVIRGHAVDSVLFNNVDIDEDAFVSQAVLMPN
                     VKVGKGAKIYRAIVADGVKIDDGAVVGSPDSEEIVLISKRVKKGE"
     gene            3262..>3605
                     /locus_tag="ACZPDN_10410"
     CDS             3262..>3605
                     /locus_tag="ACZPDN_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR1299072.1"
                     /translation="MCNALGIITYNDSSVYVEGMQKYRPIAAFSFLGRYRLVDFAISN
                     MSNSGIDDIQIYVNGNPRSLIDHVGTGRHYNINSKHGHLSLVPVYTDGENSRFTTDIS
                     CYAENIHTVMEN"
BASE COUNT         1133 a          448 c          769 g         1255 t
ORIGIN      
        1 taaatatcca ttgactcgag cttgtagagt gtcctggttg tttccatcat cgtgaaccaa
       61 taaaaatgga ggcttaaagg ctacgatcaa aaatgtattt tcgtataaaa tgtcaaccgg
      121 ttcataattt atttcaacat ctttttctgg gtaattaaaa taaatcgtct ttttctcttc
      181 atctatttta agatgctttt tatgtttttt tgaaatacaa aaagtctcaa agatttcttg
      241 agaagttgtt gtatagcgca tgaagttttc atttatattt tttatgagca tatcaataga
      301 atattttaat gatgcgtgtc ataatgtcaa tagaagaaca ggaggaatac ttatgaaaat
      361 ctttaaaaat ttagaaaacg gtagtgttgt tgaaggattg ttgatggatg ttgttcctaa
      421 tgggtatcgt gaattaaaaa cgggtgaagt ggatgcggca caagaaaagc atgttccaca
      481 agtaagtgta gaagataata aacttaaagt tgtagttgga agtgttgaac atccaatgtt
      541 agaagcacac tatatcacaa atatctgggt tgaatatagt gatggaactg tggatcgtgc
      601 aggactagta ccaggaatga aaccagaata tgtttttgat ccaaaaggaa gaacaggtaa
      661 ggtgactgtg tatgagtatt gtaacttgca tggtttgtgg aaaaatgaaa tagaattgta
      721 acggaaatgt aaaaatgata acagattgtt gaaataaaca atctgttttt ttataatagg
      781 tttatgtata aagaaaagtt aagagaatca tttaaagtat atgatgaaat cgtattaaag
      841 tgtttttgtg gaatttttat tggcgcaatt gttgcgattt gtgaagttgt ttttggcaaa
      901 ggtctagaat ggatcttaaa ttttagagaa catatgggat gtgttatgtt attaggactt
      961 ccgtttgctg gtttagccat tgtgttcttg tttgatcatt ggggaagaat ctctagaaaa
     1021 ggtatggggc ttgtctttga agttgatcaa ggaaagagtg attggattcc tcttcgtatg
     1081 gcaccgttta tggtcgtgtc gacatggatt acacatttct ttggtggtag tgctggtcga
     1141 gaaggagttg caatgcagat tggagcgact gtatcccatt atttcggtaa gtattttcgt
     1201 tttaaaaata gtggtgtcat cttcatggtt gcaggtatgg cagctggttt tgctggttta
     1261 tttggaactc caattacggc tattttcttt gccttagaag tacttgttgc gggaacctta
     1321 aaatatcgag ctatgtcatg tgcaattccg gctagtttta cagcagccta tgtttctagt
     1381 ttatttggat tacataagag tacatttaaa attggaagtg taagtgattt agatgtcgaa
     1441 ctatccataa aactattagt tttaggaata ttatttggct tgattggagg attgtttgca
     1501 ttttctttaa agcatacaaa agcttttgtg gtaaataaaa ttaaggatcc ttataaacgc
     1561 atttttatga tgggtattct tgttgcgatt ttattgtttg tatttggcaa gtgtcgttat
     1621 tctggggttg gtgaaaactt gattgtggga tcttttacat ctgaaaagat atatagttat
     1681 gattggatct taaaatttgt tttaaccatt ttaactttat cagctggttt tcaaggtgga
     1741 gaagttacac cattatttgc gattggatca agtcttggag ttataattgc gcctgttttt
     1801 gggttaaatc ctttatttgt ggcagcttta ggatattgta gcgtttttgg tgctgcaacg
     1861 aatacttttt tagcgccgat tgcgataggc atggaagtgt ttggatatca atattttcca
     1921 ttcttctttg tagtatgtgc tatagcttat atagtaaatc aaaatgaatc aatttatgct
     1981 ttacaaagac aagtcagaga atgatttgtc tttttttata gtgatttgaa aacgcataca
     2041 aatttcctta aataagtaga ttttatttga aaaagtgcta tactttagtt atataaagag
     2101 tattattagg aggatgatta tgaaacagaa taatatgctc gccatgattt tagcgggtgg
     2161 ccgtgggaca cgtcttttag aactaactaa aaagaatgcg aagccggctg tttatttcgg
     2221 aggaaaatat cgtattatcg atttcccgct aagtaactgt gcgaattcag atatcaacgt
     2281 tgtaggggtt ttaacacagt atgaatctgt agaattaaat gcttatgctg gcgctggaca
     2341 aagatgggga cttgatgcac gtggaagtgg tgtttatgtt ttgccgccac gagaaaaaga
     2401 tggaacaaaa tttgacgttt atcaaggaac ggcagatgcc attactcaaa atattgactt
     2461 tattgacaag tttgatcctg aatatgtatt gatcctttca ggtgaccaca tctataaaat
     2521 ggattatgca gatatgttat cttgtcataa ggcaaacaat gcagattgta caattgcagt
     2581 acttccagtt ccaatgaaag aagcaagccg ttttggtatt atgaatacgg atgatgaagg
     2641 aagaattgtg gaatttgaag aaaagccaga acatccaaag agtaatttag caagtatggg
     2701 tatttatatc tttaactgga aacaattgcg taaagcattg attttggaca tgactgcaga
     2761 aggctctcac catgactttg gtaaagatat tattcctaac ttcttaaacg caaataagcg
     2821 tttatatgct tacaaatttg aaggatattg gaaagatgtt ggtacaattg attcattgtg
     2881 ggaagctaac atggatttgt taaataaaaa taatgaattg aatttggatg atccaaattg
     2941 gaaaatttat acagaagata ttccaacttt acctcactat gtaggcccaa ctggaaaagt
     3001 agaacattgt tatatcaacc aaggtgctgt cattagagga catgcagtgg attccgtatt
     3061 atttaataat gttgatattg atgaagatgc atttgtatct caggctgtat taatgccaaa
     3121 tgttaaagtt ggtaaaggtg caaaaatcta tcgtgcaatt gttgcagatg gtgtaaaaat
     3181 tgatgatggt gctgttgttg gtagtccaga cagtgaagaa atcgtgttga tttcaaaaag
     3241 agtaaagaag ggtgaataat tatgtgtaat gctttaggaa taattactta caatgattct
     3301 tctgtatatg tagaaggtat gcaaaaatat cgtccaattg ctgcatttag ttttttagga
     3361 cgttatcgct tagttgactt tgcgatttca aatatgtcaa attcaggaat tgatgatatc
     3421 caaatttatg taaatggaaa tcctcgttct ttaattgatc atgttggaac aggtcgtcac
     3481 tataatatca actcaaaaca tggacattta tctttagtgc ctgtatatac agatggtgaa
     3541 aattcacgtt ttactacaga tatctcatgt tatgcagaaa atattcatac agttatggaa
     3601 aacca
//
LOCUS       DBMDWL010000191         3579 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_11536_length_3579_cov_2.004600, whole genome shotgun sequence.
ACCESSION   DBMDWL010000191 DBMDWL010000000
VERSION     DBMDWL010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11536_length_3579_cov_2.004600"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>536)
                     /locus_tag="ACZPDN_10415"
     CDS             complement(<1..>536)
                     /locus_tag="ACZPDN_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR1299073.1"
                     /translation="EGCTYNAYLVVDDEVTLFDTVEEEFTEELLERVESVLQGREIDN
                     IVVQHTEPDHSGGFKKVYAKYPNAKVYASISGKKNMIEQYFDQVPYQVVKTNDTINTG
                     KFDFVFVEMQMIHWPDNMLTYCPQLKTVFSNDAFGQHIVNFNLTDEGLDKAYCLKQAK
                     EYFANIVLPYTTPVQAKL"
     assembly_gap    537..546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            776..1915
                     /gene="nagA"
                     /locus_tag="ACZPDN_10420"
     CDS             776..1915
                     /gene="nagA"
                     /locus_tag="ACZPDN_10420"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1299074.1"
                     /translation="MIIQSKRVWVVNTWLAAQIEVEEGKIVNIYPYGQKDVDEDYGDK
                     RIIPGFIDVHTHGAYGFDTNDANEEGLRNWMKNIVKEGVTGILPTTITQSEEVLTNAV
                     KNVAKVVEEGYEGAEILGIHFEGPYLDQVYKGAQPEQYCVKPDVEQFKRYLAASNNLI
                     KIVTMACEHDDNFELTKFLRANGIVCSQGHSGATFEQARLAIANGANSMTHVFNGMTK
                     FHHREPGLVGAAMRYRDVYGEIICDGNHSTYEALNDYFTAKGPDHCIMITDSLMVKGL
                     PVGTKVLFGGNEIELYPDGSAHLTDTKGLAGSTLHVNDGLRILVEKALVPWQTAINAC
                     TLNPARMLNVDDRKGSIQTGKDADFVVLNDDYSVEMTYTKGTKAF"
     gene            complement(1934..2677)
                     /locus_tag="ACZPDN_10425"
     CDS             complement(1934..2677)
                     /locus_tag="ACZPDN_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1299075.1"
                     /translation="MNTTQRRYEIYEKLKKDKTVQVNELAKVYAVSLMTIRRDLDMLE
                     NQGLATKSYGGATLNESISTEPAFEIKEGISQSDKKEIAIFASTLIHDKDSIYLDCGT
                     TCLELFKRIHHKKITIFTNFWKILQYVDRNTKAKIIMAPGTYNPITQAALSESTIQFF
                     QNYYIDKAFISVLGIDLDYGVSIPSMSDALVKKAILASSSKKICMADHTKFNKKYMSK
                     IANIDDFDMIITDDKLESDIHAKNIIKAH"
     gene            2818..3501
                     /locus_tag="ACZPDN_10430"
     CDS             2818..3501
                     /locus_tag="ACZPDN_10430"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HIR1299076.1"
                     /translation="MELIIDSSNIEQIKELNDLLTITGVTTNPTILTKSGREAMDVVK
                     DLCEVLSEDQLLFIQTVQTSFEGIMEEAKMISSIRNKNMYVKIPVTHEGLRAIKECKK
                     LGIHTLATAIYTADQAFLAAMNGAEYLAPYTNRMCNYGDGVQDVKDLIEMLRVNHMPA
                     KVIAASFKNTYQVHELIKAGIQAVTVPCDVLYQMIDHPGTKIAVGEFSVNWQRAYNRN
                     TFEGVVTNK"
BASE COUNT         1154 a          507 c          672 g         1236 t
ORIGIN      
        1 taatttagct tgtaccggtg ttgtatatgg tagaacaata ttcgcaaagt attctttggc
       61 ttgttttaaa caataggctt tatctaatcc ttcatctgtt aagttaaagt ttacaatgtg
      121 ttgtccaaag gcatcgtttg agaatacagt ttttaattgt ggacaatatg tcaacatgtt
      181 atctggccaa tggatcattt gcatttcaac aaagacaaaa tcaaattttc ctgtgttgat
      241 tgtatcgttt gtcttaacga cttgataagg tacttgatca aagtattgtt caatcatgtt
      301 tttctttcca gaaatagatg cataaacttt cgcatttgga tatttggcat atactttttt
      361 aaatccacca gaatgatccg gttctgtatg ttgaacaacg atattgtcaa tttctcttcc
      421 ttgtagtacg gattccactc tttctaacag ttcttctgta aattcttctt ctactgtatc
      481 gaataatgta acttcatcat ctaccactaa ataggcattg tatgtacatc cttcatnnnn
      541 nnnnnntggc ggttgtttaa gtctcttact ccgacccagt atacgttatc tataattttt
      601 ttagctcttt tcattttgat tcacctcttt ctttatttta aaatttttta tgagttatgt
      661 ctattcattt gcacactttt acatttttta aataaaaaat ataaaaagta aactaaaaag
      721 tgtaagtgtt ttctttttct tgagagtata ataaaagcta ggaggaattg tcgatatgat
      781 tattcaaagt aaaagagttt gggtggtaaa tacttggttg gcagcccaaa ttgaagtaga
      841 agaaggaaag attgtaaata tctatccata tggccaaaaa gatgtggatg aagattatgg
      901 agataagaga attattcctg gatttattga tgtgcacaca catggtgctt atggatttga
      961 tacaaatgat gcgaatgaag aaggtcttcg taactggatg aaaaacatcg ttaaagaagg
     1021 tgttacaggt attcttccaa caacaattac acaatccgaa gaagttttga caaatgcggt
     1081 taaaaatgtt gcgaaagtgg ttgaagaagg gtacgaaggt gcagaaattt taggtattca
     1141 ctttgaagga ccttatttgg accaagtata caagggagct caaccagagc aatattgtgt
     1201 aaaaccagat gtagaacaat ttaaacgtta tttagctgct tctaataatt taattaaaat
     1261 tgtgactatg gcttgtgaac acgatgataa ttttgaatta actaaattct tacgtgcaaa
     1321 tggaattgta tgtagtcaag gtcactcagg agctactttt gaacaggctc gtttagctat
     1381 tgcgaatggg gcaaactcaa tgactcacgt attcaatggt atgactaaat tccaccaccg
     1441 tgaaccagga cttgttggtg cagctatgcg ttatagagat gtatatggtg aaattatttg
     1501 tgatggtaac cattcaactt atgaagcatt aaatgattac tttacagcta aagggccaga
     1561 tcactgcatt atgattacag attcattaat ggtgaaggga ttgccagttg gtacaaaagt
     1621 attatttggt ggtaacgaaa tcgaattata tccagatgga agtgcacact taacagatac
     1681 aaagggtctt gcaggaagca cattgcatgt caatgatgga cttcgcattt tagtagaaaa
     1741 ggcattggtt ccttggcaaa cagctatcaa tgcatgtaca ttgaacccgg ctcgtatgtt
     1801 aaatgtggat gaccgtaaag gatctattca aactggaaaa gatgcagact tcgttgtatt
     1861 gaatgatgac tattctgttg aaatgacata tacaaaggga acaaaagcgt tctagtcaac
     1921 aaaagcacat ttctcaatgt gcttttatta tgttctttgc gtgtatatct gattctaatt
     1981 tatcgtctgt gatgatcatg tcaaaatcat caatattggc aatttttgac atatattttt
     2041 tattaaattt tgtatggtct gccatacaaa tttttttgct tgaagaagca agaatggctt
     2101 ttttgacaag tgcatcactc atgctaggga tagatacacc atagtctaaa tcgattccta
     2161 aaacagagat aaaagcttta tcaatataat aattttgaaa gaattgaatc gtactttcag
     2221 ataatgctgc ttgtgtaata ggattatagg ttcctggagc cataatgatt ttagcttttg
     2281 tattacgatc aacgtattga agaatcttcc aaaagttagt gaatattgtg atctttttat
     2341 ggtggattct tttaaagagt tcaaggcagg ttgtaccaca atctaaatag atggagtctt
     2401 tatcatgaat gagtgtactt gcgaatatcg caatttcctt tttatcggat tgagaaatgc
     2461 cttctttgat ttcaaaagcc ggttctgtag atatagactc gtttaatgta gctcctccat
     2521 agctttttgt ggcaaggcct tgattttcaa gcatatctaa atctcttcga atcgtcatta
     2581 aggaaactgc gtatacttta gctaattcat taacttgtac agttttatct ttttttaatt
     2641 tttcatagat ttcatatctt ctttgtgttg tgttcataat tctattaaag cacaaaaaag
     2701 aacagaaaga aacatgtttt attgtgttta tttaagataa tttgtttgat gtgaaatgga
     2761 tgttgaaacg gattttatct agtgttagga tacatatgaa cttaaggagg atctatcatg
     2821 gaattgatta ttgattcatc aaatattgaa caaattaaag aattaaacga tttactaaca
     2881 attactgggg ttacgacaaa tcctacaatt cttacaaaat caggtagaga agcaatggat
     2941 gttgtaaaag atttatgcga agtgttgagc gaagatcaat tgttgtttat tcaaactgta
     3001 caaacttctt ttgaaggaat tatggaagag gctaaaatga tttcttccat tcgtaataaa
     3061 aatatgtatg tgaagattcc tgttacacat gaaggattaa gagctatcaa ggaatgtaaa
     3121 aaattaggta ttcacacatt ggctacagcc atctatactg cagatcaagc atttttggca
     3181 gctatgaatg gagctgaata tttagcacct tatacaaatc gtatgtgtaa ttatggggat
     3241 ggtgtacaag atgttaaaga tttgattgaa atgttaagag tgaatcatat gccggctaaa
     3301 gtgattgcgg caagctttaa aaatacatat caagtacatg aattgatcaa ggcaggtatt
     3361 caagctgtga ctgttccttg tgatgtttta tatcaaatga ttgatcatcc aggaacaaag
     3421 attgcggttg gtgaatttag tgtaaattgg caaagagctt ataatcgtaa cacattcgaa
     3481 ggggttgtaa cgaataagta aattttatta cttatggagt tcagctcctc tctttttgtt
     3541 tgattagctt ttacataaat aaaaaatgaa aatacacat
//
LOCUS       DBMDWL010000192         3569 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_11592_length_3569_cov_2.490773, whole genome shotgun sequence.
ACCESSION   DBMDWL010000192 DBMDWL010000000
VERSION     DBMDWL010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11592_length_3569_cov_2.490773"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            207..656
                     /locus_tag="ACZPDN_10435"
     CDS             207..656
                     /locus_tag="ACZPDN_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299077.1"
                     /translation="MMRGLHKKKTNAFILVSTVLICFLSTFINISGYYTNTKLTENTF
                     EITNANAYIFENKNNPPSLLFYLDNQYILGGYTNLKEIQDEQKLPTYKDLGKTRNIQS
                     ITNTQNTVIKNSKQTTYRWKYKLEDGYVYRRLYNACKNTWVSDWMSA"
     gene            complement(857..1951)
                     /gene="metK"
                     /locus_tag="ACZPDN_10440"
     CDS             complement(857..1951)
                     /gene="metK"
                     /locus_tag="ACZPDN_10440"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1299078.1"
                     /translation="MKDYIFTSESVTEGHPDKICDQIADAIVDAALSQDPYSNMAVEC
                     TIKDDFVLIYGECNTKAELDYETIALNEIKRIGYTEEYHVHTVVNQQSPEIHNAVNRD
                     EVCAGDQGIMFGYACDENDEYMPLTIYYAHLLARQLTKVRREHPDLLKPDGKTQVSVE
                     YENDEIKRIDTIVVSTQHSKDITQEEIKELIMNEVIKPCIDEKYLDENTKYFINPSGS
                     FVVGGSWGDSGTTGRKIVCDTYGGRGRIGGGCFSSKDPTKVDRSAAYYSRYVAKNIVA
                     NGLARRCEVEVAYAIGKAQPVSLCVDTFGTGKFDDEKLLEIVKKNFNFEVGNIISELD
                     LRKPVYHKTSCYGHFGDPQFTWEKVKKLEY"
     gene            complement(2218..2847)
                     /locus_tag="ACZPDN_10445"
     CDS             complement(2218..2847)
                     /locus_tag="ACZPDN_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HIR1299079.1"
                     /translation="MITKRNDLLSKKVIQSLRNRHMNGYYVHSKQEALDLAFEIMKPG
                     TTVGWGGSDTLNKIGIKEELYKRNFKVIDRDKARDAEEKYKLERDCFYADYYLMSTNA
                     MTEDGILVNMDGHCNRVSCLCFGPKHVIMIVGMNKVTKDIESAISRVRNIAAPINSQR
                     FAGNRPCQVTGSCANCLAQGCICDQLVITRNSMEPDRIHVILVDEVLGY"
     gene            complement(2851..>3569)
                     /locus_tag="ACZPDN_10450"
     CDS             complement(2851..>3569)
                     /locus_tag="ACZPDN_10450"
                     /inference="COORDINATES: protein motif:HMM:NF025930.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1299080.1"
                     /translation="NHSKAFYTIENNSIRMPFQVLKGVGKTIYPTIIKHVPYTDLFDF
                     LAKASMYKISESTIRILIDGGALDCFGYNRATLNENLRKLMDYVNIVRIEDPNDLRFD
                     FSIVSRPGIQRIKENPIQKAQKEQYVYGFYVSEHPVQSLRMHKYPNCIPLLNAVNRDG
                     YMNILVRVASFRTHKTKKGDWMCFMSVEDEAGKMDAVIMPRLYEQQKENIQKDRICLI
                     QGKKDRPTSIVVNRIEWIEV"
BASE COUNT         1169 a          703 c          462 g         1235 t
ORIGIN      
        1 ggtttatccc catatatttc ttcaaaaaat atattttcct atatttagaa atgaaaactg
       61 atgattctat aatcaaaaat cttgatgaaa aagaaagctt tgcctatatg cagactctga
      121 ttcaaattca gaagcaatcc tctcagaaat cattcacgtc ctgtatttct tcaaatggat
      181 ttattcttaa ttatagaatc cattacatga tgagaggtct acacaaaaag aaaacaaacg
      241 cttttatttt ggtttcaaca gtattgatct gtttcttatc tacctttata aatatttctg
      301 gatattatac aaatactaag ttaacagaaa atacatttga aatcacaaat gcaaatgcat
      361 atatattcga aaacaaaaac aatcctccat ccttattatt ttatttagat aatcaatata
      421 tcttaggtgg atataccaac ttaaaagaaa tacaagacga acaaaaacta ccaacataca
      481 aggatcttgg aaaaacccga aacattcaat caatcacaaa tacacagaat acagtcatca
      541 agaattcaaa acaaactact taccgatgga aatataaatt agaagatggt tatgtataca
      601 gaaggctata taatgcatgt aaaaacactt gggtatccga ttggatgtcc gcctaagatt
      661 tttaacgaag attaccacag tcttcgtttt tttattcaac aaggataact taaacattag
      721 aatgcctgaa aaaaaaggac ccagacaaat aaatattgtc cgagcccaat caactataaa
      781 cagttttggg gtatatactt tccgttgaaa tattggatag caaatttatc gtgccaaacc
      841 taatagttct taacgattaa tattctaatt ttttaacctt ttcccaagta aattgaggat
      901 caccaaagtg tccatagcat gatgttttgt ggtaaactgg ctttcttaaa tccaattctg
      961 aaatgatgtt tccaacttcg aaattgaagt ttttcttaac gatttctaat agtttttcat
     1021 catcaaattt acctgtccca aaagtgtcta cacataaaga tacaggttgt gctttaccaa
     1081 ttgcgtacgc aacttcaact tcacaacgac gagctaaacc attggcaaca atgtttttag
     1141 ctacataacg agaatagtaa gcggcactac ggtctacttt tgtagggtct ttactagaga
     1201 agcatcctcc tccaatgcgt ccacgtccac catatgtatc acatacgatt ttacgtcctg
     1261 tagttcctga atctccccat gatccaccta caacgaagct tccagatggg ttgatgaaat
     1321 attttgtatt ttcatctaaa tatttttcat caatacaagg tttgataact tcattcataa
     1381 tcaattcttt gatttcttct tgcgtaatgt cttttgaatg ttgtgtagaa actacgattg
     1441 tatcaatacg tttaatttca tcattttcat attcaacaga tacctgtgtt tttccatctg
     1501 gctttaacaa atctgggtgt tctcttctaa cttttgtcaa ttgacgagct aaaaggtgtg
     1561 cataataaat cgttaatggc atatactcat cattttcatc acatgcataa ccaaacataa
     1621 taccttggtc acctgcacaa acttcatcac gatttacggc attgtgaatt tcaggacttt
     1681 gttggtttac aacagtatgt acatgatatt cttcagtata tccaatacgt ttgatttcat
     1741 ttaatgcgat tgtttcataa tccaattcag cctttgtatt acattcacca taaatcaata
     1801 caaaatcatc ttttattgta cattcaacag ccatatttga atatggatct tgagataatg
     1861 cagcatctac gatcgcatcg gcaatttggt cacaaatttt atctggatga ccttcagtaa
     1921 cactttcact tgtaaaaata tagtctttca ttttgtctcc tcttctcttg cttatccata
     1981 aaaaaaacat actttaagaa gtatgttaca aatctctata cttcttatcg ctgttagcag
     2041 gaccacctta acatattgcc aggttggcgt gaagtcaacg agctaactct ctacttcaac
     2101 tctggataat ttttttacgc ataaagatta aaacatgaaa ctcaaaataa tgcaagaaat
     2161 tcatcatcat tattatacac atatgaaaaa acggctaaat tgtatagcca tttctttcta
     2221 atatcctaat acttcatcta ctaatatgac atgaatacga tcaggttcca tactgtttct
     2281 tgtgataacc aattggtcgc aaatacaacc ttgggctaaa caatttgcac aactacccgt
     2341 aacttgacac ggcctatttc cagcaaatcg ttgactgtta attggtgcag ctatatttct
     2401 tacgcgagaa atcgcactct ctatatcttt tgtgacctta ttcataccta caatcattat
     2461 gacatgttta ggcccaaaac ataaacatga cacacgatta caatgcccat ccatattgac
     2521 taaaattcca tcttctgtca tggcattcgt actcattaaa taatagtctg cataaaaaca
     2581 gtctctttct aatttatact tttcttcagc atccctagct ttatcacgat cgataacttt
     2641 aaaatttctt ttatatagct cttccttgat tcctatttta tttaaagtat ctgaacctcc
     2701 ccaacctaca gttgtacctg gtttcataat ttcaaatgct aaatccaaag cttcttgttt
     2761 cgaatgcaca taatacccat tcatgtgtct atttcttaag ctttgaatca cttttttaga
     2821 taataaatca ttccgttttg tgatcattct ttacacctca atccattcta ttctatttac
     2881 tacaattgat gttggacgat ctttcttgcc ttgaatcaaa caaattcgat ctttttggat
     2941 gttttctttt tgttgttcat acaaacgagg catgataacc gcatccattt taccggcttc
     3001 atcctctacg ctcataaagc acatccaatc cccttttttt gtcttatgtg ttctaaagga
     3061 tgccacacgc actaaaatat tcatatatcc atctctattt accgcattca ataatggaat
     3121 gcaatttgga tatttatgca tacgtaaaga ttgtacagga tgctcactta cataaaagcc
     3181 atatacatat tgttcttttt gcgctttttg aattggattc tctttaattc tttgaatgcc
     3241 cggacgagaa acaatactaa aatcaaaacg taaatcatta ggatcttcga ttcgtacaat
     3301 atttacataa tccatcagtt ttcttaaatt ctcatttaaa gttgcacgat tatatccaaa
     3361 acaatccaac gcacctccat caattaagat acgtatcgta gattcactga ttttatacat
     3421 acttgccttg gctaaaaagt caaataaatc tgtatacggc acatgtttaa taattgttgg
     3481 ataaattgtt tttcctacac cttttaagac ttgaaacggc atacgtatcg aattgttttc
     3541 aatcgtataa aaagcttttg aatgattaa
//
LOCUS       DBMDWL010000193         3517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_11848_length_3517_cov_1.960187, whole genome shotgun sequence.
ACCESSION   DBMDWL010000193 DBMDWL010000000
VERSION     DBMDWL010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11848_length_3517_cov_1.960187"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="ACZPDN_10455"
     CDS             complement(<1..1372)
                     /locus_tag="ACZPDN_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1299081.1"
                     /translation="MTKILKYLKPYWLSIVLAFGLLFGQATCELTMPDYMQNIVNTGI
                     QNAGIESGVYQQIREKTLNSYLMFANDSEKDLITSSYKLVTPSKATKEEKTEIPALKT
                     ENVYVLKGIGEEKEEKLSSTLTLLQMTMVEVSKDAKEQNVQIPQLLMMNGIDSYRKDA
                     KSEISKLGDSTVSSMSSQFLKKEYTDLGMNMEKMQQDYIIYSGLKMLGYAFGSAVCAI
                     LVGFLASRIAAGFSKDLREEVFEKVTHFSNENLNTFSTSSLITRTTNDIQQLQMTIVM
                     FLRIVMYAPIIGVGAILHVIESGANMTWILALCVVVILSVVLLIFMIVMPKFKIMQKM
                     IDKMNSVVRELLDGMLVIRAFNNEKLEEKYNSEIKERMMKIPNIMHESVPLGEDDSKN
                     VEIEKFGDPVVPPYEVPYHTDIMESLSGIDLDSARRVAGNGFYYLVGDIARLHSAVIS
                     YARDFMI"
     gene            complement(1369..1806)
                     /locus_tag="ACZPDN_10460"
     CDS             complement(1369..1806)
                     /locus_tag="ACZPDN_10460"
                     /inference="COORDINATES: protein motif:HMM:NF047172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1299082.1"
                     /translation="MEVQDEVLDLMFDLTYKVTNYVRNNRHCKDGPRIKDVMLFKTIY
                     KAENHQITMSELANILGVSAAAVSQMIAGFEKKGLLQRVHSNTDRRTVYIQIVPEAIE
                     MFQKRMDTHVENVYRFLDYLGSEDCAHLRDILIKTTRYMEENK"
     gene            complement(1999..2421)
                     /locus_tag="ACZPDN_10465"
     CDS             complement(1999..2421)
                     /locus_tag="ACZPDN_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025825.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR1299083.1"
                     /translation="MYDQEKIKKMKNHELAMEAYFYAYNHKLFGGASQKKAVKCYEQI
                     IPILDSDERINLPFISTYGRCYVATEKRLIKCTKKLFGYEIEERNWNEIKHIFYTSTA
                     SRGALILDTVYGEVKIGVHRDGAGYACEVLRKLKEASK"
     gene            complement(2761..>3517)
                     /locus_tag="ACZPDN_10470"
     CDS             complement(2761..>3517)
                     /locus_tag="ACZPDN_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299084.1"
                     /translation="NVKSKSVQSTMLIEYFSFFDDNRDSRLSEEALKICKDTNDITLR
                     RTTWQYLYNCYGSEYIECMILPFADENLLFEINFKCKNISKGKMCEAMEREYIKEYSL
                     RLQADLIKSGSTKALNDYVQKVSRDKRPVEDKFNRFDGPTEAIASIKNPEFLPQLKIL
                     LETVLDKAYTDNEYVNLKGSLTQALINCGGCAYQKTTRIIKELQPSIEEDEDNFVYCN
                     YILNGIETERKRVVDKVYHFKAVKKLLNENASI"
BASE COUNT         1125 a          694 c          449 g         1249 t
ORIGIN      
        1 ttatcataaa atctctggca tatgatataa cagcagaatg taatcttgcg atatctccta
       61 ctaaataata gaatccattt cctgcaaccc tacgtgccga gtccaaatct ataccagata
      121 aagattccat aatatctgta tgatatggaa cttcataagg aggtacaact ggatctccaa
      181 atttttctat ctcaacattt ttgctgtcat cttctccaag gggaacagat tcatgcataa
      241 tgttaggaat tttcatcatt ctttctttta tttctgaatt atatttttct tcaagctttt
      301 cattgttgaa agcacgaatg actaacattc catctaacaa ttcacgaacc acactattca
      361 ttttatcaat cattttctgc atgatcttaa attttggcat gacaatcata aagatcaata
      421 aaacaacact taaaatcaca acgacacata aagctagaat ccaagtcata tttgcacccg
      481 attcaatgac gtgtaagatg gctcctacac caatgattgg tgcatacatt acgattctta
      541 aaaacatcac aattgtcatt tgaagttgtt gaatatcatt tgtagtacgt gtaattaaac
      601 ttgatgtact aaatgtgttc aagttttcat ttgagaaatg agtgactttt tcaaacactt
      661 cttcacgcaa gtctttggaa aagcctgcgg caattcttga agctaaaaag ccaactaaaa
      721 tcgcacatac agcactgcca aaggcatagc ctaacatctt aagtcctgaa tagataatat
      781 agtcttgttg catcttttcc atattcatac ctagatcagt atattctttt ttaaggaatt
      841 gagaactcat gctcgataca gtcgaatctc ccaatttaga aatctcggat tttgcatctt
      901 ttctatagga atctatacca ttcatcataa gaagttgtgg aatttgtaca ttttgttctt
      961 tagcatcttt actcacttca accattgtca tttgaagtaa ggtcaaagtg gaagaaagct
     1021 tttcttcctt ttcttcacca atacctttta atacatatac attctctgtt ttcaaagcgg
     1081 gaatttcagt tttttcttcc ttggttgcct tacttggtgt aaccaactta tatgaagatg
     1141 taatcaaatc cttttcagaa tcatttgcga acattaaata ggaatttaaa gtcttttcac
     1201 gaatttgttg ataaacccca ctttcaatac ccgcattttg aattcctgta ttgacaatat
     1261 tttgcatata atccggcatt gtaagctcac aagtcgcttg tccaaacaac aaaccaaacg
     1321 caagtacaat ggatagccaa tagggtttta aatatttcaa tatctttgtc atttattttc
     1381 ctccatataa cgagttgtct tgatcaatat atctcttaaa tgtgcacagt cttcggatcc
     1441 taaataatcc aaaaaacgat atacattttc tacatgtgta tccattcttt tttgaaacat
     1501 ttcaatggct tcagggacaa tttgaatata gaccgttcta cgatccgtat tcgaatgcac
     1561 cctttgaagc agccccttct tttcaaatcc agcaatcatc tgcgaaacag ctgcagcaga
     1621 tacacccaat atatttgcca gttcactcat cgtaatctga tgattttctg ctttataaat
     1681 cgttttaaac aacatcacat cctttattct aggaccatct ttacaatgac ggttatttcg
     1741 aacatagttc gtgactttat acgtgagatc aaacatcaaa tcgagtactt catcttgaac
     1801 ttccataaga acctccaatt tatttaagtt acttaactat atacctactt aactattaag
     1861 tcaataaaaa agatatgcat aaaacatatc tttcaatgga cctatgaaac tctttaatcg
     1921 aaagccaaaa gatttaaaga gagattccaa aggcttctcc gaccttatta tatcacatca
     1981 aaactgtgat acatccattt attttgaggc ttcctttaat tttctcaaca cctcacaagc
     2041 ataacctgcg ccatctcgat gaactccgat tttaacttca ccataaacag tatccaatat
     2101 taaagcacct cttgatgcag tactagtgta gaatatgtgc ttaatttcat tccaattccg
     2161 ttcttcaatt tcatacccaa acaatttctt agtacatttt atcaaacgct tttctgttgc
     2221 cacataacac cttccatatg tagaaatgaa aggtaaattg attctttcat ccgaatccag
     2281 aataggaata atctgttcat agcacttaac agcctttttt tgagatgccc caccaaataa
     2341 tttatgatta tatgcgtaga aataagcttc catagcaagc tcatgattct tcatcttttt
     2401 aattttctct tgatcataca taacaggttc cctttcattt agtcagtaat tccctaaaca
     2461 tttattatca ataatattat taacacaatt gattcgaaga atatatagtg agttgcacaa
     2521 ttaagaaaat atggtatatt aattaggacg aaggtgacgc aagaggcgca cgccagcttc
     2581 tgtacgcaga ggctgttttt tttattttca gattctcaaa atcaaaaatc gaggcataac
     2641 ctgcatatat gcacatcata cctcgctaaa agctacttag cttttgttcc taatgttagt
     2701 ttacacttca acccactaaa cagtcaatct ttaaaaggta tatttcatat tcaagatttt
     2761 ttagattgaa gcattttcat taagcaattt ttttactgct ttaaagtgat aaactttatc
     2821 aactactctt tttctctctg tttctatacc attgagaata taattgcaat aaacaaaatt
     2881 atcctcatct tcttctattg atggttgtag ttctttaatt attctagtag ttttctgata
     2941 agcacaacca ccacaattta ttaaagcttg cgttaatgat ccttttagat tcacgtattc
     3001 attatctgta taagccttat ctaaaactgt ttctagtagt atttttaatt gaggtaaaaa
     3061 ttcagggttc ttgatacttg cgatagcttc tgttgggcca tcaaatcgat taaatttatc
     3121 ttcaaccggt cgcttatccc tactaacttt ttgaacataa tcatttaaag ctttcgtact
     3181 tcccgatttg attaaatcag cctgtaatct aagagaatat tctttaatat actctctttc
     3241 cattgcttca cacatttttc ctttcgatat atttttacat ttaaaattaa tttcaaacaa
     3301 taaattctca tcggcaaatg gaagtatcat acattcaata tactcagatc cataacaatt
     3361 atataaatat tgccatgtag ttctacgaag agtaatgtca tttgtatctt tacatatttt
     3421 tagagcttct tcacttaatc gtgaatccct attgtcgtca aaaaacgaga aatactctat
     3481 caacattgta ctttgcacag attttgattt tacattt
//
LOCUS       DBMDWL010000194         3460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_12135_length_3460_cov_2.662102, whole genome shotgun sequence.
ACCESSION   DBMDWL010000194 DBMDWL010000000
VERSION     DBMDWL010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12135_length_3460_cov_2.662102"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /gene="mnmE"
                     /locus_tag="ACZPDN_10475"
     CDS             complement(<1..1092)
                     /gene="mnmE"
                     /locus_tag="ACZPDN_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1299085.1"
                     /translation="MFENTIAAISTSLQDGAISIIRLSGDQAIEITQKIFDRNIMNAK
                     SHTIHYGFIIDPDKNPVDEVLISIFRAPKTYTREDIVEINCHGGTFITRKILSMVLSA
                     GADLAKPGEFTQRAFYHGRIDLSQAEAVQDMIEASNNTAATMAIHGIKGSVKKLLQPL
                     IDDLMDIIAQIEVNIDYPEYEDVEQLTTNDLLPKTNDWLEKIDHILSRVQTGQMLKKG
                     IDTIIVGKPNVGKSSLLNALLEEDKAIVTDIAGTTRDLVEGQIHIGSVQLNLIDTAGI
                     RESNDKIEQIGIEKSQEKLKDAKLVLLVFDGSKELDEEDKQLLELTKDKMRLIIYNKL
                     DKAEANKDGIWISAANKDIQPLIDALENLY"
     gene            1165..1761
                     /locus_tag="ACZPDN_10480"
     CDS             1165..1761
                     /locus_tag="ACZPDN_10480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1299086.1"
                     /translation="MIEYTKNQVHEVACAFNEHKIIALPTDTVYGVGVKYGNLDDLER
                     LKRAKHRPETKPIPMMVSNIEQMKQVALVDERIEKIAAKFLPGALTLVVNVKENVDPA
                     YTNGLSTIAIRIPDEKFILDVIDELNCPILVTSANQSGEKTALTSDDVYEQLPKIDGI
                     VLGTCKALQASTIVDCTKDKLVILRPGPISLEELESVQ"
     gene            1948..2196
                     /locus_tag="ACZPDN_10485"
     CDS             1948..2196
                     /locus_tag="ACZPDN_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299087.1"
                     /translation="MTEEEMIREIAEPILEQLKKIEKQLGNHRMSQLPQIKFVKEGNM
                     QDGPFMIGDIEVTDELLEKVEAYVQEEIEMMHQPTVLH"
     gene            2282..2365
                     /locus_tag="ACZPDN_10490"
     tRNA            2282..2365
                     /locus_tag="ACZPDN_10490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Leu,seq:tag)
     gene            2428..3333
                     /locus_tag="ACZPDN_10495"
     CDS             2428..3333
                     /locus_tag="ACZPDN_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR1299088.1"
                     /translation="MEICVYYEKINEDSIRIKRVYASTPTIEIPEFIDGYIVREIGNY
                     CFSKKEVDLSNSVLSHEIPSSYYECSGSDVESVKLSKTVTKLGDYAFYNCRKLKEIFL
                     PSSLICIGSDVFMNCLRLNHIYYDCSIFCITILKQILTQITWDIEVDFIDGSIFYPEY
                     NGGYDEVGPAHIFALNIEGEGFRMRQCFKDGKIDFDGYDACFEKLCAEESESCIFHVA
                     ILRFMMGSERYVPYLRAHDLTSYLHTYKDICVMVEKLIEEKCMDAQALDVLISMEKDL
                     ETRTLLMELKNKKMETSSAYSFEDF"
BASE COUNT         1128 a          532 c          651 g         1149 t
ORIGIN      
        1 atacaagttt tccaaggcat caatcaaagg ttgaatgtct ttgttggcag ctgaaatcca
       61 aatgccatct ttatttgcct ctgccttatc taatttatta tagataatta agcgcatttt
      121 atcttttgtc agttctaata attgtttgtc ttcttcatcc aattctttgg aaccatcaaa
      181 gactaaaaga accaatttgg catcttttaa tttttcttga ctcttttcga taccaatctg
      241 ttcaatctta tcatttgatt cacgaatgcc cgctgtatca attagattta attgaacgct
      301 accaatatgg atttgacctt caaccaaatc tcttgtggtc ccggcaatat ctgtaacaat
      361 ggctttatct tcttcaagaa gggcattcag taagctggat ttaccaacat ttggcttacc
      421 aacaataata gtatcaattc ctttttttaa catttgcccc gtttgaacac gagaaagaat
      481 gtgatcaatt ttttctaacc aatcatttgt tttgggtaac aagtcattcg ttgttaattg
      541 ttcgacatct tcatattcag gataatcaat attcacttcg atttgtgcaa taatatccat
      601 taaatcatca atcaaaggtt ggagtagttt ttttacactt cccttaattc catgaatcgc
      661 catagtagcc gctgtattgt tgctcgcttc gatcatgtct tgaacagctt cagcttggga
      721 taagtcaatt cttccatggt agaaggcgcg ttgcgtaaat tctcctggct tggcaagatc
      781 ggctcccgca cttaaaacca tggataagat tttacgtgta ataaaagtac ccccgtgaca
      841 gttgatttct acaatatctt ctcgtgtata tgtttttggt gctctaaaaa tagagattaa
      901 aacttcatcc actggatttt tatctggatc tataataaag ccataatgaa tcgtatgact
      961 tttcgcattc ataatattac ggtcaaatat tttttgagtg atttcaatag cttgatctcc
     1021 acttaaacga atgatggaaa tggcgccatc ttgtaaagaa gtggaaatag ccgcaattgt
     1081 gttttcaaac atagaaaaac atcctttctt tcttagtgta ttttatcata aaagatgtat
     1141 aatgtcttag acagaggtgt aaacatgata gaatatacaa aaaatcaagt acacgaagtg
     1201 gcgtgtgcat ttaatgaaca taaaattata gctttaccta cagatactgt gtatggtgta
     1261 ggtgtaaagt atggtaattt agacgattta gaacgattaa aacgcgcaaa acatcgtcca
     1321 gaaacgaaac ccattccgat gatggtttca aatatagaac aaatgaaaca agttgcttta
     1381 gtggatgaaa gaatcgaaaa gattgccgct aagtttttgc caggcgcatt aactttagtt
     1441 gtcaatgtga aagaaaatgt cgatccagcg tatacaaatg gcctaagtac gattgcgatt
     1501 cgtattccag atgaaaaatt tatcttagat gtgattgatg aattgaattg tccaatctta
     1561 gttacaagtg ccaatcaatc tggagaaaag acagcactca caagtgatga tgtatatgaa
     1621 caacttccaa aaatagatgg aattgtttta ggtacgtgca aagcattaca ggcaagtaca
     1681 attgtcgatt gtacaaaaga taagctagtt attttaagac caggacctat ttcattagaa
     1741 gaattagaat cggttcaata aaaagcaccc agaacttatg ttgaaaagca taggccaggg
     1801 tgcgtatgaa caaatctaga aatagtatgc ttctagcagt tcatgtagta tgttactctg
     1861 atttaaattg gattcaatgg tttcgaatgc aatttaaaca tatgttaaaa cttgtcaaaa
     1921 tgatgggggt ttattataat actgagtatg acagaagaag aaatgattag agagattgcc
     1981 gaacctattt tagagcaatt gaagaaaatt gaaaaacaac taggaaatca tcgtatgtca
     2041 cagcttccac aaattaagtt tgtgaaagaa ggcaatatgc aagatggccc ttttatgatt
     2101 ggagacatcg aagttacgga tgagctttta gaaaaagtag aggcttatgt tcaggaagaa
     2161 atcgaaatga tgcatcaacc gactgtttta cattaagaag ttacaaaaat gtagcttctt
     2221 ttttaaaaaa tgcttgcatt ttattttgcg ttagtgtatt atatgtgagc acgttggaaa
     2281 tgcgggtgtg gcgaaattgg cagacgcacc agacttagga tctggcgcct tcggcgtggg
     2341 ggttcgagtc ccttcacccg caccacttgt aaatttagac aattacttcg gtagttgtct
     2401 ttttttgttt tatgttataa tttccatatg gaaatttgtg tatattatga aaaaataaat
     2461 gaagattcaa ttcgtatcaa gcgtgtgtat gcatcaactc ccacaataga aatacccgaa
     2521 ttcattgatg gatatattgt gcgtgaaata ggaaactatt gtttttctaa aaaggaagtc
     2581 gatctatcta attctgtttt aagtcatgaa attccttcta gttattatga gtgtagtggt
     2641 agtgatgttg aaagtgttaa gctttctaag acagtcacaa aactagggga ttatgcattc
     2701 tacaattgtc ggaagctaaa agaaatattt ttaccttcta gtttaatatg tattggcagt
     2761 gatgtattta tgaattgttt aagactcaat catatctatt atgactgttc aatattttgt
     2821 atcacaattt taaaacaaat actaactcaa atcacttggg atatcgaagt cgattttata
     2881 gatggttcta tattctatcc agaatacaat gggggttatg atgaagtggg accggcccat
     2941 atctttgcct tgaatattga aggggaaggc tttcgtatgc gacagtgttt taaagacggt
     3001 aaaattgatt ttgatggtta tgatgcctgt tttgaaaaat tatgtgcaga agaaagtgaa
     3061 tcttgtatct ttcatgttgc cattcttaga tttatgatgg gctctgaaag atacgttcct
     3121 tatttaagag cacatgattt gacttcttat ttacatacat ataaagatat atgtgttatg
     3181 gttgaaaaat taatagaaga aaaatgtatg gatgcacaag ctttagatgt attgatttct
     3241 atggaaaaag atttggaaac tcgaactcta ttgatggaat taaagaataa gaagatggaa
     3301 acatcatccg catattcttt tgaggatttt taatgcgaca aagtgaatgg gaaaaggaaa
     3361 aggcaattca tatcttagaa ttagttcgat ccgaactcta tatagatatg ccacattttt
     3421 taacagcttt aaatacgctg atgttgaagg aagacgagcg
//
LOCUS       DBMDWL010000195         3447 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_12200_length_3447_cov_2.844889, whole genome shotgun sequence.
ACCESSION   DBMDWL010000195 DBMDWL010000000
VERSION     DBMDWL010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12200_length_3447_cov_2.844889"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    286..295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <296..967
                     /locus_tag="ACZPDN_10500"
     CDS             <296..967
                     /locus_tag="ACZPDN_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1299089.1"
                     /translation="FINDLYFMVGAYQSYVYSCRAKNYQQKKSYQILGIYMAVMLIVG
                     TLQDIFRQVPIFCVGTSLSILIVYISLEEQMISIDPLTQLNNRNRMEQHLFECMRNAD
                     ANMYLLVLDVNRFKKINDEHGHAQGDLALKAIANCLKESCIEKSDFIARYGGDEFVIV
                     YKGNDVEGLCQRIHTYIQQLSFPFDLDVSIGYAQYKKEMRKWNDWFIKADKQLYDEKK
                     KLKGR"
     gene            complement(984..1622)
                     /locus_tag="ACZPDN_10505"
     CDS             complement(984..1622)
                     /locus_tag="ACZPDN_10505"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1299090.1"
                     /translation="MKVIDLTHTISEAMPVYPGTEKPKLTAVNTYSKDGFKETLIQIY
                     SHVGTHMDPPAHIFANQKTLDQFSPEQFIGKALVIDCRDLKEGQSIPIDKINMDIAAS
                     ADFLLFNLGWDKRWKDESYFNDYPCIDDEVLEFILQGNFKGIGFDVIGLDPIHDENLT
                     RHKALFKNRDIVNIENLKNLDQCGKDLFWFSCFPLKIENCDGSPIRAVAWYD"
     gene            1697..2833
                     /gene="dxr"
                     /locus_tag="ACZPDN_10510"
     CDS             1697..2833
                     /gene="dxr"
                     /locus_tag="ACZPDN_10510"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1299091.1"
                     /translation="MKRILLLGATGSIGLQTVDVIEQHPDQFCLVGIAAGHQADVMQK
                     IIDKHPSIQVAGISDVSKANQLKGVKIFSGTNAMVDCIENCEYDLLVNAVVGFRGLKP
                     TLTSLKKGIDVALANKESLVAGGVLVRKTLHETNTKLYPIDSEHSAIFQSLQGNRNED
                     VKRLIITASGGSFRDKYRDELSDVTVEQTLKHPNWNMGKRITVDSATMVNKGFEVIEA
                     HYLFDLPYEKIDTVLHRQSIVHSMVEYNDNAIIAQLGSADMRLPIQYALCYPDRPVLK
                     EDHPLDMTKTMDLNFKEMDYVRYPMLKLAYEIGKKEGNLGAVFNGADEQAVQLFLEKK
                     ISFLQIEESIEQACKNAKYIENPTIEQLEASDAWARKFVIEYWD"
     gene            2885..>3447
                     /locus_tag="ACZPDN_10515"
     CDS             2885..>3447
                     /locus_tag="ACZPDN_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299092.1"
                     /translation="MNKTKVYCVASAVAVCVASNPSMDVLAKHLQPVKLEEKAQQTIS
                     AEDYIKYYLSTKEIVKDLNNKDIEKYTLITKVDEKNCTIVLTGNQLFKELTKENQDEI
                     KNAYETAYKDAGMKKAKNCTLSAYDIIVSQASKMVLDAKEQLSLLVKDAESLDASLYT
                     TSTYASLKSEVNESYNLIAQDSTTLEQV"
BASE COUNT         1198 a          445 c          668 g         1126 t
ORIGIN      
        1 atattatatt ttcaaaccga tttagtacga atgagtaatg tacagaatct ttttttgaat
       61 gaaatgagtt ttctgttctt ttcattgtgt actttttcat ggtttaacta cattttaaca
      121 atgttagaaa tacaatatgt caaaaaaaca caaacgctta tagctttaat tcctttgatt
      181 gtaagtgttg ttttattgtt aatgaatcca ttaaatggca tattgtttaa aattgatcaa
      241 aatgcaaatt ttcaagaagg actattctat cttttgtatg tatttnnnnn nnnnntttat
      301 aaatgatctc tattttatgg ttggagctta tcaatcttat gtgtatagtt gtagggcaaa
      361 gaattatcaa caaaagaaat cctatcagat tttagggata tacatggctg ttatgttgat
      421 tgtaggtacg cttcaagata tttttcgcca ggttcctatc ttttgtgtag gaactagtct
      481 gagtattctg attgtatata ttagtttgga agaacaaatg atttctattg atccattaac
      541 acaattgaat aaccgtaatc gtatggaaca acatttgttt gaatgcatga gaaatgcgga
      601 tgcgaatatg tacttgttag tgttggacgt gaatagattt aagaaaatca acgatgagca
      661 tggacatgcg caaggggatt tagcattaaa ggccatcgcg aactgtttaa aagaatcttg
      721 tattgaaaaa agtgatttta ttgctcgtta tggtggcgat gaatttgtga ttgtctataa
      781 aggaaatgat gtagaaggat tatgccaaag aattcatact tatatacaac aattatcttt
      841 tccgtttgat ctagatgtaa gtattggata tgcgcaatat aaaaaagaaa tgcgtaaatg
      901 gaatgattgg tttatcaaag ccgataaaca attatatgat gaaaagaaga aattaaaagg
      961 ccggtaaatt aaattgccgg tctttaatcg taccaagcga cagctcgaat gggtgagcca
     1021 tcgcagtttt caattttaag tggaaaacaa ctaaaccaaa ataagtcttt tccacattgg
     1081 tctaggtttt taagattttc aatattcaca atatctcgat ttttaaataa agctttgtgc
     1141 cgagttaaat tttcatcatg aataggatct aatccaatca catcaaaacc tatgccttta
     1201 aaatttcctt gaagaataaa ttctaatact tcatcatcaa tacatggata atcattaaag
     1261 tatgactcgt ctttccatct tttgtcccaa cctagattaa ataaaagaaa gtctgcagat
     1321 gcagctatat ccatattgat tttatcaatt gggatggatt gtccttcttt taaatctcta
     1381 caatcaatga ctagagcttt accgatgaat tgttccggtg aaaattgatc caatgttttt
     1441 tgattcgcaa agatatgggc aggtggatcc atatgtgttc ccacatgaga atatatttga
     1501 atcagtgttt ctttaaatcc atcttttgaa tatgtattta cggcagtgag ttttggtttt
     1561 tcggttccag ggtaaactgg catagcttca gaaatcgtat gtgttaaatc aatgactttc
     1621 ataagtttcc tcctaaagat atttaaccac aatttttgtt gtattgtgat aaaattatag
     1681 atacaggtga ttagttatga aaagaatatt attattaggg gctacaggct caattggttt
     1741 acaaactgtg gatgtgattg aacaacatcc agatcagttt tgtctagtag gaattgcggc
     1801 tggacatcaa gcggatgtga tgcaaaaaat cattgataaa catccatcga ttcaagttgc
     1861 tggtatttcc gatgtttcaa aggcaaatca attaaagggc gtaaagattt ttagtggaac
     1921 gaatgccatg gtggattgta ttgaaaattg tgaatatgat ttattagtga atgcggtggt
     1981 tggatttaga ggcttaaaac caactcttac atccttaaag aaaggtatag acgttgcctt
     2041 agccaacaaa gaaagtttgg tagcgggtgg tgttttggtt cgtaagacat tgcatgaaac
     2101 aaatacaaaa ttatacccaa ttgattcgga acattcagct attttccagt ctttacaagg
     2161 caatcgtaat gaagatgtaa aacgtttgat tattacggca agtggaggta gttttagaga
     2221 taaatataga gatgaattaa gtgatgtaac agtagaacag actttaaaac atccaaactg
     2281 gaatatgggt aaaagaatta cagtggatag tgcaacaatg gtgaataagg gatttgaagt
     2341 gattgaagct cattatttat ttgatttgcc ttatgaaaaa atcgatacag tattacatcg
     2401 tcaaagcatt gtacactcta tggttgaata taatgataat gcgattattg cacagcttgg
     2461 ttctgcggat atgcgcttac ccattcaata tgcactatgt tatccagatc gtccggtatt
     2521 aaaggaggat catcctttgg atatgacaaa gacaatggat ttgaatttta aagaaatgga
     2581 ttatgttcgt tatccaatgt taaaactagc ctatgaaatt ggtaaaaaag aaggaaatct
     2641 aggtgctgta tttaatgggg cggatgaaca ggccgtacaa ttattcttag agaagaagat
     2701 ttctttttta caaattgaag aaagtattga acaagcttgt aagaacgcaa aatatattga
     2761 aaatcctaca attgagcaat tagaagcatc ggatgcatgg gcaagaaagt tcgtcattga
     2821 atattgggac tagaccaatt gaaattttgg gagtaaaata gtgaaatgga atggagggtt
     2881 tgtcgtgaat aaaacaaagg tttattgtgt agcaagtgct gttgcggttt gtgtggcttc
     2941 aaatcctagt atggatgtac ttgcaaaaca tttacagcca gtaaaattag aagaaaaagc
     3001 ccaacaaaca atttcagcag aagattatat taagtattat ttgagtacaa aagaaattgt
     3061 taaagatctt aacaataaag atattgaaaa atatactttg atcacaaaag tagatgaaaa
     3121 gaattgtaca atcgtattga ctggtaatca attgtttaag gaattgacaa aagaaaacca
     3181 ggatgaaatt aagaatgcgt atgaaactgc atataaagat gcaggtatga aaaaggcaaa
     3241 gaattgtaca ttaagtgcat atgatattat tgtaagtcaa gctagtaaaa tggttttgga
     3301 tgcgaaagaa caattgtcac ttttagtaaa agatgcagaa agtttagatg cctctttata
     3361 tacgacaagt acatatgcat cattaaaatc tgaagtgaat gaatcatata atttgattgc
     3421 acaagatagt acaactttag agcaggt
//
LOCUS       DBMDWL010000196         3442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_12223_length_3442_cov_2.199940, whole genome shotgun sequence.
ACCESSION   DBMDWL010000196 DBMDWL010000000
VERSION     DBMDWL010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12223_length_3442_cov_2.199940"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="ACZPDN_10520"
     CDS             complement(<1..277)
                     /locus_tag="ACZPDN_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299093.1"
                     /translation="MDDTNRYTHYLVLFLCSMIIPAMNTSAVSIALSNNIVDLIFYII
                     DFGVFGWGIQRLKEKDEHPISIILAVTAIEDLIISFMCNITFITYIVQ"
     gene            complement(316..981)
                     /locus_tag="ACZPDN_10525"
     CDS             complement(316..981)
                     /locus_tag="ACZPDN_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR1299094.1"
                     /translation="MWNRQQVKEQAKQIMKRNYWKMFVVALIAGILSGDYVTVIQAVQ
                     DFVPDVVLPSMFSSILSFLSMGSIVGYLFSIFIGNVIVVGKSRYFIKNHDVNPELGEI
                     FSGFKGNYLNVVKIMFLMNLKILLWLFLFIVPGFIKAYEYSMIPYLLAENPNISADEA
                     FSLSKQMTTGQKMDLFVLDLSFLGWIILGLICCGIGILFVLPYPEATNAEVYLNLKES
                     VKI"
     gene            complement(1063..1404)
                     /locus_tag="ACZPDN_10530"
     CDS             complement(1063..1404)
                     /locus_tag="ACZPDN_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299095.1"
                     /translation="MCVKFHDEENRLEIIGGASCLYEEIQKCSIQNEDANFKGKTKPF
                     THTILGGATFMAGAVEPMMYVGIKVVLKDNSVLPIYVSKEKVLFNSDKYLNDVKEANA
                     ILKELEKRLQS"
     gene            complement(1385..1747)
                     /locus_tag="ACZPDN_10535"
     CDS             complement(1385..1747)
                     /locus_tag="ACZPDN_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299096.1"
                     /translation="MNLNNYVKFDDSCKQFEILTGTQGKYSYDDVTRVSVLNEKAKYK
                     GKGIPFTAVLPGGPLPSGLLQNPYLYVGVKIVLKNDTVLAIYVSKEKTMVNTDQYIQD
                     RKIAKKIEEMIKNVCEIS"
     gene            complement(1790..2443)
                     /locus_tag="ACZPDN_10540"
     CDS             complement(1790..2443)
                     /locus_tag="ACZPDN_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299097.1"
                     /translation="MKKYAAALLSVFLVCGCSNITPNNEKVEASQEKLENMTALYTGF
                     AEANETIDNKSKIGVWAYYEDGTKEFITEGWTIKEPVTLEAGKTSEVTVEYRGLESTI
                     MVECSEVDEASFKAESQSPDQSDLEVTHSKWYGKKLTYTGKIIARVDDKDFRGYMINI
                     FDDKSYVCVLDFNKEFDFKEGQTVTFWAYGLGRVETKGLFGKERSCIAFKAKYMEEV"
     gene            complement(2477..3118)
                     /locus_tag="ACZPDN_10545"
     CDS             complement(2477..3118)
                     /locus_tag="ACZPDN_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1299098.1"
                     /translation="MISLKGVSKSYKGITIFEDVNITFMPNKWNFIEGVNGSGKSVLL
                     KLICGFSKPDTGIVTVDDYIIGQDYDFIQNAGICINSPDFFNNLSGMDNLLEIAKIKK
                     IVTKNEIYQFCKLFGLEEDINKKYKYYSLGMKQKLRIIQAIMESPNILILDEPFDALD
                     KQAKQIMQNYLNDFIKKENHYLIYTSHSNEHKEFADVIYSIDAKTIKQIKNVE"
BASE COUNT         1163 a          675 c          377 g         1227 t
ORIGIN      
        1 cctgaacaat atatgtaata aaagtaatat tgcacataaa acttatgatt aaatcctcta
       61 tagctgttac agctaatata atactaatag ggtgttcatc cttctctttt aaccgttgaa
      121 taccccatcc aaacactcca aaatcaatga tataaaaaat taaatctacg atattattcg
      181 aaagtgctat tgatactgca gatgtattca ttgcaggaat aatcatagaa caaagaaata
      241 aaactaaata atgagtgtat ctatttgtat catccataaa aatatctcct aaattattgg
      301 tactagtatc cgtccttata tctttacaga ctcttttagg tttaaataca cttcagcgtt
      361 tgtggcttct ggatatggca atacaaatag aataccaatt ccacaacaaa ttaagcctag
      421 aatgatccat cctaaaaatg ataaatctaa gacaaataaa tccatctttt gtccagtcgt
      481 catttgtttg gacaaagaga aggcttcatc agccgaaatg tttggatttt ctgccaatag
      541 atatggaatc atagaatatt cataagcctt aataaatcca ggtactatga acaggaatag
      601 ccatagtaat atttttaaat tcatcaaaaa catgattttt actacattta aatagttgcc
      661 tttaaaacca ctaaagattt caccaagctc aggatttaca tcatggttct tgataaaata
      721 tctacttttt ccaactacaa tgacattacc aataaagata gaaaataaat atcctacaat
      781 agatcccatt gataaaaatg aaaggattga agaaaacata cttggcaata caacatcagg
      841 tacaaaatct tgtactgctt gaataacagt cacataatca ccactcaata ttcctgcaat
      901 cagcgcaacc acaaacatct tccaatagtt tcgtttcatg atttgtttag cttgttcttt
      961 aacttgttgc ctgttccaca tattatttca ccttactttc tttatactta atatcgattc
     1021 ttcaattttt ttgttacacc ctatgcctcg aatgcaacaa ttttaagatt gcaatctctt
     1081 ttctaattcc ttcaatattg cattggcttc tttaacatca tttaaatact tatcactatt
     1141 aaataacact ttttccttgg aaacatatat tggtaataca ctattatctt ttaaaacaac
     1201 tttaatacca acatacatca ttggttctac tgcaccagcc ataaatgtag ctccacctaa
     1261 aattgtatgt gtaaatggtt ttgttttacc tttaaaatta gcatcttcgt tttgaataga
     1321 acacttttga atctcttcat ataaacagct tgcaccacca atgatttcta atctattttc
     1381 ttcatcatga aatttcacac acatttttaa tcatctcctc tatcttcttt gcaatcttac
     1441 gatcttgtat gtactgatct gtattaacca ttgtcttttc tttcgacaca taaatagcta
     1501 atacagtatc attctttaaa acaatcttca ctccaacata caaatatgga ttctgtaata
     1561 agccagatgg taatggccca cctggaagca cagccgtaaa tggaattcct tttcccttat
     1621 attttgcctt ttcatttaga acagatactc ttgtcacatc atcataagag tattttcctt
     1681 gtgttccagt aagaatctca aattgtttac aagaatcatc aaatttaaca taattattta
     1741 aattcataag tttagcctcc ttaccatttt caattcttca aatacgcact tatacttctt
     1801 ccatgtattt tgccttaaac gcaatacaac ttctctcttt tccaaacaaa ccctttgttt
     1861 ccacacgccc aagaccatat gcccagaacg taacggtttg accttcttta aaatcaaatt
     1921 ctttattgaa atcaagcaca caaacataag acttatcatc aaaaatattt atcatatatc
     1981 ctctaaaatc tttatcatcc actcgagcaa tgatttttcc tgtatatgtc aatttctttc
     2041 cataccactt agaatgtgta acctctaaat ccgattgatc tggtgattgt gactccgctt
     2101 taaaggacgc ttcatccacc tcactgcatt caaccataat cgtactttct aaaccccgat
     2161 attcaacagt aacctctgaa gtctttccag cttctaatgt gacaggctct ttgatagtcc
     2221 atccttccgt aatgaattct tttgtaccat cctcataata ggcccataca ccaatcttcg
     2281 acttattatc tatagtttca ttcgcttctg caaaacccgt atataaggct gtcatattct
     2341 ctagcttttc ctgactagcc tctacttttt cattatttgg tgttatattc gaacatccac
     2401 aaaccaaaaa tacacttagc aatgccgctg catacttctt cataggtcca cctccttttt
     2461 tatatgaata tttttgttat tcaacatttt taatttgctt gattgtttta gcatctatgc
     2521 tataaattac atctgcaaat tctttgtgtt cattcgaatg actggtatat attaaataat
     2581 gattctcttt tttgataaaa tcattcaggt aattttgcat gatttgttta gcttgtttat
     2641 ctaaagcatc aaaaggctca tctaatatta gaatatttgg actttccatt attgcttgaa
     2701 taatcctaag cttttgcttc atacctaaag aataatactt atatttttta ttaatatctt
     2761 cctctaatcc gaataattta caaaattgat aaatttcatt ttttgtcact atctttttta
     2821 ttttagcgat ttccaataaa ttatccattc cagataaatt gttaaaaaaa tctggtgaat
     2881 ttatacagat ccctgcattt tgtataaagt catagtcctg tccaataata taatcatcta
     2941 ccgtaactat acccgtatct ggtttactaa atccgcatat aagctttaat aaaacgcttt
     3001 tccctgatcc gttcactcct tcaatgaagt tccacttatt gggcataaaa gtaatattta
     3061 catcttcaaa aattgttatg cctttataag attttgaaac accttttaaa ctaatcataa
     3121 ataatactca ctttgattta tgtaaaattt tctgatgaga caataggata aaactgacgt
     3181 cacaattatt atgcaaaaat atattccttc aaaaacgcta tttgaactca ttgtcactaa
     3241 atttgtttta gaatagttat caagaaatgt catcaatatc aaaaaaagaa attgtagata
     3301 tacaaaaaac tctttcttgt ataatatctg aaaaatcgtt cgcaaaaaga taatctcaaa
     3361 caacagcgaa aagaaacgaa taagatacat ataaatcata aataagtttg aaacatcaca
     3421 atgttcaacg gcacaaatga aa
//
LOCUS       DBMDWL010000197         3438 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_12252_length_3438_cov_2.521127, whole genome shotgun sequence.
ACCESSION   DBMDWL010000197 DBMDWL010000000
VERSION     DBMDWL010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12252_length_3438_cov_2.521127"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="ACZPDN_10550"
     CDS             <1..228
                     /locus_tag="ACZPDN_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299099.1"
                     /translation="LIKKFRIVIIFNGIKYVWGLFWVVKALNSKSVYLVISCLKWLSL
                     GLFISMLIVSIVLLYTLSKMIADYDDFVHEL"
     gene            433..1686
                     /locus_tag="ACZPDN_10555"
     CDS             433..1686
                     /locus_tag="ACZPDN_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HIR1299100.1"
                     /translation="MMNFIEKHSKILAVSLCLLTSGPIVSTMYAKEVQPGWHGEGADR
                     YYILESNRQTATGLTKIDTELYYFNSKGEMQFGWQNVSDETYYFQADGTAATGTVTIH
                     GSEYNFQKEGVLLQGWSEDGKSFYDEKGNKTSTNWVEDNGAKYYFDDKGNCVTGWQKI
                     DGQDYYFGEDGKMATGQFDVDGKTYFTGEDGIFRTGWQVINGQDYYYAENGEVTKNKF
                     LDIDGTKYAFDENGNLIKNTEKDGYKIDENGVATEITPSQDSSTQDTTSTPAQSTTTN
                     TNTSTSTNTSTNTTQAPSQNTTGGSTNNSYNNNYAPSTPSVNSSAIAAAALNQVGASQ
                     DCTMLVTNALAAVGINFHGSPEQYLSLGRITSSPVAGDIIVYSGHVAIYLGNGQAVHG
                     GWLGNQTVVGPVSCGNALIAYVHVG"
     gene            1755..2954
                     /locus_tag="ACZPDN_10560"
     CDS             1755..2954
                     /locus_tag="ACZPDN_10560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR1299101.1"
                     /translation="MNYIQTQKFSDVYVSCKTMMPFNRNTITALNVLVYMMKAKTEKM
                     DSKQKLASTLNLAYGTKISYGLTSFGDLIQVDVRFQFVRPDWIEDENYVNKIKDIMDQ
                     ALFHSVLDEENFKESVYLLKNRLLAQMDDPANLSCMYAFEMAHDKHSISIPVQGSLKD
                     LETLTLKEVKQIYTLYMDMAKHFYICGYVDQELYDFIDSLDSHCPFISERTLLPKVET
                     SYKTFEKDISQTCISQVYSTGVDISSADYEAELLLCSILGQSQKNLMFDEIREKNSLC
                     YSISSSIIRFDGAILIHTGVNRKDVTKVLNLIETQIDRLLNMDYDDAYLEIAKMGFKD
                     RIIGGLDQALSLIAQAFLDDLLHRKLTTEQRIERIMKVTKEDISRVALQMNLASVAIV
                     AENENEL"
     gene            2944..>3438
                     /locus_tag="ACZPDN_10565"
     CDS             2944..>3438
                     /locus_tag="ACZPDN_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012879.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR1299102.1"
                     /translation="MNYNLDLTKETLPNGLQVLLVHKPDYQRSLFLLGAKVGGFDIDQ
                     NVDGTLVHHKSGLAHYLEHQMFYLDGEDVSELFAGLQCSTNAYTSYTETAFYFSTTAD
                     VKKPLKLLFDFVENLDVTNKTIEKEKGIILSEYDMYQQSPEQRLFKETLISLFQYHPM
                     KVDVL"
BASE COUNT         1182 a          481 c          667 g         1108 t
ORIGIN      
        1 ttgataaaaa agtttcgaat tgtcattatt tttaatggaa ttaaatacgt ttggggcttg
       61 ttttgggttg ttaaagcatt aaattcaaaa agtgtatatt tggtaatttc ttgtttaaaa
      121 tggctttctt tagggctttt tataagtatg ttgattgttt ctatagtttt actgtatacg
      181 ctttcaaaaa tgattgcgga ttatgatgat tttgttcatg aattgtagac aatattatgc
      241 atttactaaa ttgtgtacaa ttttttataa aatcacacaa tttaccacaa tttagaatca
      301 tatgctttat ataaagctta ttaaggaaaa attaagaaat aaattactaa aaagtgcaaa
      361 tgatttttaa gcgaaaattt gcaacaaaaa agaatccttg ttaaacttca gatgttgtaa
      421 cgaagaggtg atatgatgaa ttttatcgaa aaacatagta aaattttagc cgtaagccta
      481 tgtctattga cttctggtcc aattgtttcg acaatgtatg ccaaagaagt tcaaccagga
      541 tggcatggtg aaggcgcaga tcgttattat attttagaat caaatcgtca aacagctact
      601 ggtttaacaa aaatcgatac tgaactttac tatttcaata gtaagggtga aatgcagttt
      661 ggttggcaaa atgtttcgga tgaaacatat tatttccaag cagatggaac tgcagcaaca
      721 ggcacggtta ctattcacgg cagtgaatat aatttccaaa aggaaggtgt tcttcttcag
      781 ggatggagtg aagatggtaa atcattctac gatgaaaaag gaaacaagac ttcaacaaac
      841 tgggttgaag acaatggcgc aaaatattat tttgacgaca aaggaaattg tgtaacgggt
      901 tggcaaaaaa tcgatggcca ggactattat tttggtgaag atggaaagat ggctacaggt
      961 caatttgatg tagacggaaa aacatatttc actggggaag atggaatctt ccgtactggt
     1021 tggcaagtga tcaacggtca ggattattat tatgcagaaa atggcgaagt cactaagaat
     1081 aaattcttag atattgatgg aactaaatac gcattcgatg aaaatggtaa tttaattaag
     1141 aataccgaaa aagatggata taagatcgat gaaaatggtg ttgcgactga aataactcct
     1201 tctcaggatt cttcaactca agacactaca agtacaccgg ctcaaagtac tactacaaac
     1261 acaaacacaa gtacaagtac aaacacaagt acaaatacta cacaagctcc ttcacagaat
     1321 acaacaggtg gttctacaaa caatagttac aacaataact atgcaccatc aactccaagt
     1381 gtaaactctt cagctattgc ggctgcagcg ttgaatcaag taggagcaag tcaagattgt
     1441 acaatgttag tcacaaatgc tttagccgct gtaggaatta atttccacgg ttctcctgaa
     1501 caatatttaa gtttaggacg cattacatca agtccagttg caggtgatat tattgtttat
     1561 tcaggtcacg ttgcaattta tcttggaaac ggacaagctg ttcatggtgg atggctaggc
     1621 aatcaaacag ttgttggccc tgtttcttgt ggtaatgcat tgatcgcata tgttcatgta
     1681 ggttagaatt caattctaac cttttttttg tttttgtggt aaaatcaata atatattttt
     1741 caaggaggtc ttgtatgaac tatattcaaa cgcaaaagtt tagcgatgtc tatgtttctt
     1801 gtaaaacaat gatgcctttt aatcgaaata caattacagc actcaatgtt cttgtatata
     1861 tgatgaaggc aaaaacagaa aagatggatt caaaacagaa attagcttct acgctaaatc
     1921 tagcttatgg tacgaaaata tcttatggat taacatcttt cggagattta attcaagtgg
     1981 atgtgcgttt tcaatttgtt cgtccagact ggattgaaga tgagaattat gtaaataaaa
     2041 tcaaagatat tatggatcaa gctttatttc attcggtatt ggatgaagaa aacttcaaag
     2101 agtctgtata tctattaaaa aatagattat tagcacaaat ggatgaccca gccaacttat
     2161 cttgtatgta tgcctttgaa atggctcatg acaagcattc gatttcgatt cctgtacagg
     2221 gaagtttaaa ggatttagaa acgcttactt taaaagaggt caaacaaata tatacactct
     2281 atatggatat ggctaaacat ttctatattt gtggatatgt tgatcaagaa ctatatgatt
     2341 ttattgattc tttggattcg cattgtcctt ttatttcaga aagaacattg cttccaaaag
     2401 tagaaacgag ttataagaca tttgaaaagg atatttctca aacctgtatt tcgcaagtgt
     2461 attctacagg tgtggatatt tcaagtgcag actatgaggc agaattgctt ttgtgtagta
     2521 ttttaggaca aagtcaaaag aatttgatgt ttgatgagat acgtgagaag aatagccttt
     2581 gttattcaat aagctcatcg atcattcgtt ttgatggtgc gatattgatt catacgggag
     2641 taaatcgtaa agatgttact aaagtattga atttaattga aacacaaatc gatcgattat
     2701 tgaatatgga ttatgacgat gcctatttag aaattgcgaa aatgggattt aaggatcgta
     2761 ttattggtgg attggatcag gcactctctt taattgccca agcgttttta gatgatttgt
     2821 tgcatcgtaa actaacaaca gaacaaagaa ttgaaagaat tatgaaggtg acaaaggaag
     2881 atatttcgcg tgtcgcctta caaatgaatt tggcatcagt agccattgtt gcggagaatg
     2941 aaaatgaatt ataatttgga tttaacgaaa gaaactttac caaatggctt acaagtttta
     3001 cttgtgcata agccggatta tcaaagatca ctctttcttt tgggggcaaa agttggtggc
     3061 tttgacatag atcaaaatgt ggatggaaca cttgttcatc ataaaagtgg attggctcat
     3121 tatttagagc atcaaatgtt ttatttagat ggagaagatg ttagtgaatt gtttgcgggt
     3181 ttgcagtgtt ctacaaatgc ctatacttca tatacggaaa ctgcatttta tttttcaacg
     3241 acagcagatg taaaaaagcc attaaagctg ttatttgatt ttgttgagaa tctagacgtg
     3301 acaaacaaaa cgattgaaaa agaaaaggga attattctaa gtgaatatga tatgtatcaa
     3361 caatcgcctg aacaacgtct ttttaaagaa actttgattt ctttatttca atatcatcca
     3421 atgaaagtgg atgtactc
//
LOCUS       DBMDWL010000198         3400 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_12470_length_3400_cov_3.548348, whole genome shotgun sequence.
ACCESSION   DBMDWL010000198 DBMDWL010000000
VERSION     DBMDWL010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12470_length_3400_cov_3.548348"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="ACZPDN_10570"
     CDS             <1..680
                     /locus_tag="ACZPDN_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299103.1"
                     /translation="LGEYEAYLENELSNSEFSKKIIQRFDTINQLHQYYEDFSITFDS
                     MSEILNESNPHKYIKSIYAKLVDISDIPHINDIENIKENEIKKIKDWTNRKSETAYNI
                     FNEENLPDMLNYGESITIIGIDDSKFTLNIDEIDIFPTEGSEEIIDIGLCNNKNEVIR
                     GYIKLIVGYLNFDEDGGASDGTSDEIIYEYKDILNALDEFISQQNCIVEDEKQVVEII
                     KSVLLSD"
     gene            744..1310
                     /locus_tag="ACZPDN_10575"
     CDS             744..1310
                     /locus_tag="ACZPDN_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299104.1"
                     /translation="MIDENLMKFIENYPKLVKSLISHNLFNFNLRKRLKSFSKILQSS
                     DFSLEQSNDIFTEYFDATVCLRTNYLNATCPVEINISKLRKKVINKPTVNIVTEQLYN
                     LVLDSTKNEYDPLFNSNINDEPVLLLHDKIFDYQIIDGNHRTIRNYQCNNYYTGAVML
                     NTFDILDCIDNNIYKEIVNLFVDIYDIL"
     gene            1523..1813
                     /locus_tag="ACZPDN_10580"
     CDS             1523..1813
                     /locus_tag="ACZPDN_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299105.1"
                     /translation="MKLNEEHFYILRHQMVQYPTVNNNADLEFLSTPQCKVRLYVELL
                     TTSKLSDYHTDLNKQAETMFSRTVYPLNRKVAVTDGGFSFSGSIQEVIQFRE"
     gene            1968..2156
                     /locus_tag="ACZPDN_10585"
     CDS             1968..2156
                     /locus_tag="ACZPDN_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299106.1"
                     /translation="MTNGNATIIVGSAGQEHSAVFSARQVLFHTIYNEPIAGDRAHYA
                     LASAFCVCRKDGGLILKS"
     gene            2263..2523
                     /locus_tag="ACZPDN_10590"
     CDS             2263..2523
                     /locus_tag="ACZPDN_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299107.1"
                     /translation="MKKKLLSLLLALCFVMALVPMTAFAEGTSVDNWDGTAGQEHSAV
                     FSARQVLFHTIYNEPIAGDRAHYALASAFCVCRKDGGLILKS"
     gene            2630..>3400
                     /locus_tag="ACZPDN_10595"
     CDS             2630..>3400
                     /locus_tag="ACZPDN_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299108.1"
                     /translation="MKKKILSLLLALCLVMALVPMTAFAEGTSVDSWDGTADTRWYTD
                     HKTDTEYHFTTAEQLAGLAQLVNDKTASVSFEGKTIYLDNDLDLSGSQWTPIGDGSNH
                     VRFFAGTFNGQHHKIMNLSHHYTGNEVVRNGLFGVVSDGGTLKNLLVIDADIDSNDGS
                     LIAGILADWVNGGTVENCYTSGKIENNVGSKFVGGLIGQCTWSTQVKGCGSDATVIST
                     ESNEDDVDTVGGLIGQWENSADSSSITDCWFGGSVSCNN"
BASE COUNT         1115 a          516 c          694 g         1075 t
ORIGIN      
        1 ctcttgggga atatgaagct tatttagaaa atgagttgtc caattcagaa ttttctaaaa
       61 aaataattca aagatttgat acaattaacc aattacatca atattatgaa gatttttcaa
      121 ttacttttga ttcaatgagt gaaatactta atgaatcaaa tccacacaaa tatattaaat
      181 caatatatgc aaaattagtt gacatatcag atattcctca tattaacgat attgaaaata
      241 taaaagaaaa tgagatcaaa aaaataaaag attggacaaa tcgtaaatct gaaacagcgt
      301 ataacatttt taatgaggaa aatcttcctg atatgcttaa ttatggggaa agtattacta
      361 taataggaat tgatgacagc aaatttactc tcaacatcga tgaaattgat atattcccaa
      421 ctgaaggaag tgaagaaatt atagatatag gtttatgcaa taataaaaac gaagtgatta
      481 gaggatacat aaaattaatt gttggttatc taaattttga tgaggatggc ggtgcttctg
      541 acggcacaag tgatgaaatt atatatgagt ataaagatat attgaatgca ttagatgaat
      601 ttatttctca acaaaattgt attgttgaag atgaaaagca agttgttgaa ataatcaagt
      661 cggttttact gtcagattaa taaaaatact acattgaata attaatcatg cttgaattta
      721 tagaacaatg gggatatata tagatgattg atgaaaatct aatgaaattt attgagaatt
      781 atcctaagct tgttaaatcg ttgatatcgc ataatttatt taattttaat cttagaaaaa
      841 gattaaaatc attttctaaa atattgcaat cttctgattt ttctttagaa caatcgaatg
      901 atatttttac agaatacttt gacgctaccg tatgtttaag aacaaattat cttaatgcca
      961 cttgtccggt tgaaattaat ataagtaaat taagaaaaaa agtaatcaat aaacctactg
     1021 taaatatagt tacggaacag ctctacaatt tagtacttga ttccaccaaa aatgaatacg
     1081 atccattatt taactctaat ataaatgatg aacctgtttt attattgcat gataaaattt
     1141 ttgattatca gattattgat ggaaatcata gaacaattag aaattatcag tgtaataatt
     1201 attatactgg tgcagtaatg ttaaatacat ttgatatact tgactgtatt gataataata
     1261 tttataaaga aattgttaat ctgtttgttg acatttatga tattctttag tctagcgaaa
     1321 taacataatt atagcagtag atattaaata tgttataaat gatattaata gactatatat
     1381 aatttgttga ttataaaaat ttgagcaatc gtgcagaagt gaatggttat ttggactgaa
     1441 aacaaattgg agtgtaacta aaagaacggc ttattatgtt taagttaggt atttatttgc
     1501 cgccatatat gacattttgt ttgtgaaact aaacgaagaa cacttttata ttcttcgtca
     1561 tcaaatggtg cagtacccta ctgtaaataa taatgcagat ttagagtttc tttcgacacc
     1621 tcagtgtaag gtccgcttgt atgttgaact gctgactacc agcaagctga gcgattacca
     1681 tacagacctt aacaagcagg cagaaacaat gttttctcgg acggtttacc cgctaaatag
     1741 aaaagtcgct gtcacagacg gcggcttttc cttttcggga agtattcaag aagtcataca
     1801 gttccgtgag taatggaaga aggcaatgat ttgagaaaaa gagaagtgta atattctgaa
     1861 aacttcttga gattttggga aaaatatgct acaatacttt tgtataatta tatagtgggg
     1921 aatttatatc ctttttgcct taggaattat ggaaggcgga cttgtctatg acaaacggaa
     1981 atgcgacaat cattgtcggc tcggctgggc aggagcatag cgccgttttt tcagcgcgtc
     2041 aagtcttatt tcatacaatt tataacgaac caattgccgg agacagggcg cattatgccc
     2101 tggcttcggc tttttgcgtt tgccggaagg atggtggttt gatattgaag tcctaaccac
     2161 gaccggcaaa tcgatctttt ccggttaaac cttctgtctt ctccggcgtg tccgctgtcg
     2221 gagaaagtaa atagcggata gaattatttt ggaggaatga aaatgaagaa gaaacttcta
     2281 agccttctgc ttgcgttgtg ctttgtgatg gcacttgttc cgatgacagc gtttgctgaa
     2341 ggaacttcag tagataattg ggacggtacg gctgggcagg agcatagcgc cgttttttca
     2401 gcgcgtcaag tcttatttca tacaatttat aacgaaccaa ttgccggaga cagggcgcat
     2461 tatgccctgg cttcggcttt ttgcgtttgc cggaaggatg gtggtttgat attgaagtcc
     2521 taaccacgac cggcaaatcg atcttttccg gttaaacctt ctgtcttctc cggcgtgtcc
     2581 gctgtcggag gaagaaaata gcggatagaa ttattttgga ggaagaaaaa tgaagaagaa
     2641 aattctaagc cttctgcttg cgttgtgcct tgtgatggca cttgttccga tgacagcgtt
     2701 tgctgaagga acttcagtag atagttggga cggtacggca gatacacgct ggtacaccga
     2761 ccataagacg gatactgaat accatttcac cactgccgaa cagttggccg gtttagcgca
     2821 gttagtcaat gacaaaactg catctgtatc atttgaagga aaaacgattt acttagataa
     2881 tgacctcgac ttatccggct cccagtggac tcccattgga gatggcagta accatgtaag
     2941 attttttgct ggtactttta atggacagca tcataaaatc atgaatctga gccatcacta
     3001 tactggaaat gaagttgttc gaaatggact tttcggagtt gtttctgacg gtggtacatt
     3061 aaagaattta cttgttatag acgctgatat tgactcaaat gatggttcat taattgctgg
     3121 cattttggcg gattgggtca atggcggcac cgttgaaaac tgctacacaa gcggaaaaat
     3181 agaaaacaat gttggcagca agttcgttgg tgggctgatt ggtcaatgca cttggagtac
     3241 acaggtaaaa ggctgtggct cagacgccac ggttatcagt acagagtcca atgaagatga
     3301 tgttgatacc gttggcgggc tgattggtca gtgggaaaac tctgcggaca gctcgtcgat
     3361 taccgattgt tggtttggcg gttctgtttc ctgcaataat
//
LOCUS       DBMDWL010000199         3343 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_12812_length_3343_cov_2.782233, whole genome shotgun sequence.
ACCESSION   DBMDWL010000199 DBMDWL010000000
VERSION     DBMDWL010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12812_length_3343_cov_2.782233"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            230..613
                     /locus_tag="ACZPDN_10600"
     CDS             230..613
                     /locus_tag="ACZPDN_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299109.1"
                     /translation="MISFNKSLLYSDKDIKSSENNYGYLEYKENGKIYTFTIKDEIVS
                     ISGIVDKRNQRNIFKIPTSELKNDHVQIKFEKSSEKYFISTIADDTRLNQRKIPQSIG
                     TPVWVEMPNNSEIFMSGIFSVKFKK"
     gene            625..1743
                     /locus_tag="ACZPDN_10605"
     CDS             625..1743
                     /locus_tag="ACZPDN_10605"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR1299110.1"
                     /translation="MGNRQLIIFSGVSSMGRIRTNNEDAFYVNYIWDEDNILAIVADG
                     LGGCEKGEVASNLAVETIVTYLTTYKNGDRLELLRQAVIAANNIIIEKQKELPDASNM
                     STVLTVSIFDSVAHLVYMAHVGDTRLYQFTHNEGLIKLSHDHTLVGYKEEQGELTEIE
                     AMSHPKRNEISKLLGATFVDVNSQNFVELNCYDFLSQSCYLLCSDGLYDLITSNEIGK
                     ILDGNDSVTEKSQKLIEDANAAGGKDNITVVLIENHNQWDEKINTHSNDLRQKNIETT
                     NSPKRNIINRNYLLCLLYLVIGIVIGASIYFFVDNIYIKHANIGAVVQTDTIFIETNS
                     DSIELLPNNQDSVIRKLEEDLMLLKSVLESKKTNTTHK"
     gene            1767..2432
                     /locus_tag="ACZPDN_10610"
     CDS             1767..2432
                     /locus_tag="ACZPDN_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299111.1"
                     /translation="MIVLTIIFLLIVPYVYEIFWIIFINPKISVISQLGIYQWAAVGF
                     VAYCIFHKYVRLNIEWLETDSHEKIHGIVGWLFGRRIHSAHSEEGSGCIYTSGNNVFS
                     RWGMLPMSLAPYCLPLWTYSILWIRPLIGYHGLWIFDILVGITLAFHYFCFKSQTRSN
                     QTDINQYPLSFSYVYIYLARIINGLIIIVMFFPQSDSIKYPIMRLLKAILHNVEVLLR
                     III"
     gene            2442..2897
                     /locus_tag="ACZPDN_10615"
     CDS             2442..2897
                     /locus_tag="ACZPDN_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299112.1"
                     /translation="MTNDKENYIGKQYVENKEANEIFSFLEGQMSNFSAQFNGMCKKV
                     TYSIFASSWATIFALRDNEYRIWLLITILLCILFFMIDIIYNFAMEQFSRKLHYKFEH
                     GNLETEDVDKRWNVVSDFAGKWLQISKLVIIAIMVIILAYYYFLAYKMS"
     gene            2936..>3343
                     /locus_tag="ACZPDN_10620"
     CDS             2936..>3343
                     /locus_tag="ACZPDN_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299113.1"
                     /translation="MKVKYLLAMIALLVLASCHHKTNSEMSGNDSIVDISEISITDTT
                     IIDDEEKIIHAKMMYYDFDKIDNLESFFDSILAKHPIPIWLPEEDKTTQKVKECIAKI
                     GEFRKGIKQFFPDSLVSECLKSIGFNTAIVNNHG"
BASE COUNT         1183 a          476 c          608 g         1076 t
ORIGIN      
        1 ttgggggatt ttttattatt ttatgcaaag atacaaaatt cttataacac tgacaattag
       61 atcgttaggt tgtttttgac tgatttacga gcatccctat ggtagtaatg ttggctcatc
      121 ttcttctgta aaagtatctg tgtcatgttt gaattctgac ccaagtaagt ccaccgaaat
      181 taatacagat acactaaaag gtattaatct tataggagac aatcactatt tgatttcgtt
      241 caataaaagc ctactttata gtgataaaga tataaaaagt agtgaaaaca actatggata
      301 tcttgaatat aaagaaaatg gtaaaattta cacttttacc ataaaggatg aaatcgtaag
      361 tatctcggga attgttgaca aaaggaatca gcgaaatatc tttaagatac ctacatcaga
      421 attaaaaaat gaccacgttc aaattaaatt tgagaaatcc tctgaaaaat atttcatttc
      481 aacaatagct gatgatacaa gattaaatca gcgaaaaatt ccccaaagta tcggtactcc
      541 tgtttgggtt gaaatgccta ataactctga gatttttatg agtggtattt tctctgtaaa
      601 atttaaaaaa taagcatata taacatggga aatcggcagt tgataatatt ctcgggtgtg
      661 tcttccatgg ggcgaatccg cacaaataac gaagatgctt tttacgttaa ctatatatgg
      721 gacgaggaca acatattagc tattgttgca gatggattag gaggctgtga gaaaggggaa
      781 gttgcctcaa acttggctgt tgaaacaatt gtaacttatc ttactacgta taagaatgga
      841 gacagattgg aactattgag acaagcagta attgctgcaa acaatatcat aatagaaaag
      901 caaaaagaac ttcccgatgc gtccaacatg agtacggtat taacagtgtc aatatttgat
      961 tctgtggctc accttgttta tatggctcat gttggagaca ctcgtcttta ccaatttacc
     1021 cataatgaag gacttataaa actctctcat gatcacactt tagttggata caaagaagag
     1081 caaggagaat tgacggaaat tgaagcaatg tcgcacccaa agagaaatga aataagtaaa
     1141 ttattaggag cgacatttgt tgatgtcaac agtcaaaatt ttgtagagct taattgctat
     1201 gattttctgt cacagtcatg ttatcttctc tgctctgatg gtttatacga cctgattacg
     1261 tccaatgaaa ttgggaaaat tttagacggc aatgatagtg ttaccgaaaa gtcccaaaaa
     1321 cttatagaag atgcaaatgc ggcaggaggt aaagataaca taacggttgt gttgatagaa
     1381 aaccataacc aatgggatga aaaaattaat acgcatagca atgatttacg acaaaagaat
     1441 atagaaacta caaattcccc aaaacgaaat atcataaata ggaattattt attgtgctta
     1501 ctctaccttg ttatcggtat agtcattggg gcttctatat atttctttgt tgataatata
     1561 tatataaaac atgccaatat aggtgctgtt gtacaaactg acacaatttt catagaaact
     1621 aatagcgaca gcatagaact tctgccaaat aatcaagaca gtgtaattcg taaattagaa
     1681 gaagacttaa tgttgcttaa atcggttttg gaatcaaaaa agactaatac aacgcataaa
     1741 tgagtaaaag gagttatata ttatggatga tagttctaac tattattttc ctattgatag
     1801 ttccatatgt ttacgaaata ttctggatta tattcattaa tccaaagatt tctgtaataa
     1861 gccaattggg aatttatcaa tgggctgctg tcggatttgt cgcatactgc atatttcaca
     1921 aatatgttag attaaatata gaatggcttg aaactgattc acatgaaaaa atacatggaa
     1981 ttgttgggtg gttatttgga agacgtatac attcggctca ctcagaagaa ggtagtggat
     2041 gtatctatac tagtggaaac aacgttttta gtagatgggg aatgttgcca atgtcattag
     2101 ctccatattg tttacctctt tggacttatt ctattttgtg gattagacca cttataggct
     2161 atcacggtct ctggattttt gatattttag taggaattac attagcattc cattacttct
     2221 gtttcaaatc tcaaaccaga agtaatcaaa cagatataaa ccaataccct ctttctttct
     2281 cgtatgtata catatatttg gcaagaataa taaatggatt gataatcata gtaatgttct
     2341 ttccccaatc agactcaata aaatatccaa taatgcgatt gctaaaagca attttgcata
     2401 atgttgaagt tttattaaga atcataattt aaatatttcc aatgactaat gataaagaaa
     2461 attatatagg taaacagtat gtagaaaata aagaagctaa tgagattttt agtttcttag
     2521 aaggacaaat gagtaatttt tctgcacaat tcaatggaat gtgcaagaaa gttacatatt
     2581 ctatttttgc ttcctcttgg gcgacaattt ttgctttaag agataacgaa tatcgtattt
     2641 ggttgttgat aacaatcctg ttatgtattc tattttttat gatagatata atttacaatt
     2701 ttgcgatgga acagttctca cgaaaattgc attacaaatt tgagcatggc aatcttgaga
     2761 cagaagatgt cgacaaaaga tggaatgttg taagtgactt tgctggaaaa tggttgcaaa
     2821 tatcaaaact tgtaataatt gctattatgg tgataatttt agcttactat tattttctag
     2881 catataaaat gtcctaagat agacaaagtg gtcggtatga actaatggca acagaatgaa
     2941 agtaaaatat ttattggcaa tgatagcttt gcttgtgttg gcaagttgtc atcataagac
     3001 aaatagtgag atgtctggta atgattctat tgtggacata tcagaaataa gtattactga
     3061 tactaccatc attgatgatg aagagaagat aatacatgct aagatgatgt attatgattt
     3121 cgataaaata gataacttag aatcattttt tgattctatt ttagcaaaac atcctatccc
     3181 tatttggcta ccagaagagg ataagacaac tcaaaaggtg aaagagtgta tcgcaaagat
     3241 aggggaattc cgcaagggta ttaaacagtt ctttccagac agtcttgtga gtgaatgttt
     3301 gaaatccatc ggattcaaca cggctatcgt caataatcat gga
//
LOCUS       DBMDWL010000200         3301 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13055_length_3301_cov_3.168125, whole genome shotgun sequence.
ACCESSION   DBMDWL010000200 DBMDWL010000000
VERSION     DBMDWL010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13055_length_3301_cov_3.168125"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(5..1768)
                     /locus_tag="ACZPDN_10625"
     CDS             complement(5..1768)
                     /locus_tag="ACZPDN_10625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1299114.1"
                     /translation="MENQVTQLLKAMVKPDYTACSYAFMQDGKIVCADAIGVIDKINN
                     QPITTDCTFNVCSISKIYCTVCVMQLVDEGLIQLEDKVADILPKFTMKDERYKDITVR
                     MCLDHSSGLPGTQWKHFSVSTTSKFDYYSEVLNYLSNSTLKADPGTYSTYCNDGFTLA
                     EMVVSKVRNMPYEDVLRKYITEKIGAKSTNTTYTIHSDYPLVSEGKKPKEYFPIQGAG
                     GITTSMIDLCKFGQLFLEPNSIISEKSKALMAKSWGTTFLESDLGAIDFGLGWDLVRH
                     HDPDYDFGDGVLAKGGNSMFFSSRLIIVPKYNAVLALSETHDCGLEVPTTLMRLFNTY
                     LEPNTYPDYSGIYAHAFGLQKITTIKSSMVVQDKTEKGWIMSDLLNYADGKWTNEKGN
                     QIFFEGDYLLKTTRNRTVAFAQKAKKQELNSVWKSRLNKKYIVCDTTYYDIVTNQMLC
                     SVEFNRTEDTLSLIVHGNKSEPVVSEFPIEVIDDTHAKSYLNTPCNGSRDRIEPYFED
                     DKLYCASYTYICEDDIEPYNFQLFEKENQVYKINNTLEVLPMICENHRILVLDANGDL
                     YYDSMDVEEYKPIESGFIILV"
     gene            complement(1814..3097)
                     /locus_tag="ACZPDN_10630"
     CDS             complement(1814..3097)
                     /locus_tag="ACZPDN_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1299115.1"
                     /translation="MKYGLIGEHLGHSFSKQIQTRIAEIENVKDYDYQLVELDKDEFK
                     EFMEKKDFKGINVTIPYKKDVIPYLDEMDESAKAIGAVNTIINVDGKLKGFNTDFGGF
                     LYMVKAHNVHMEGKKVLIIGNGGACAAVKAVCEHENAKDIVIVSRSANRGAISYDEMY
                     ASHLDADIIVNTSPVGMYPNIMNAPVDVSWFHKLECVLDVVYNPILTRLCFEAQEADI
                     KRVVGLEMLIAQAKYTFEIFENMSFDDSIIDEIKKEMLKDRCNIVLIGMPSAGKTTIG
                     KMLEEKLGKEFIDLDDMIIAKAGKSIPEIFQESGEAGFRAIETEVAMEASKMNNKIIA
                     TGGGVVKHKINMDFLRLNGITIFIDRDIDKLISSDPNRPLSSSKQALQQMHKERYPLY
                     QKYAAYVAVNNANVEETVDDIVNAYHSILIDTVAE"
BASE COUNT         1004 a          628 c          501 g         1168 t
ORIGIN      
        1 aaggttaaac caagatgata aatccagatt caataggttt atattcttcg acatccattg
       61 aatcatagta taaatctcca tttgcatcta atactagaat tcgatgattt tcacaaatca
      121 ttggtaagac ttctaaagtg ttgttgattt tgtagacttg attttctttt tcaaacaatt
      181 gaaaattata tggttctata tcatcttcac aaatataggt gtagctagca caatataatt
      241 tatcatcttc aaagtaaggt tcaatacgat ctctagaacc gttacaaggc gtgtttaaat
      301 agcttttggc atgtgtatca tcgattactt caattggaaa ttcagatacg actggctcgg
      361 atttattacc atgtacaatc aatgatagtg tatcttctgt cctattaaat tctacactac
      421 acaacatttg gtttgtgaca atatcataat aagttgtatc acatacaata tatttcttgt
      481 ttaatctgga tttccagact gagttgagtt cttgtttttt ggctttttgt gcaaacgcaa
      541 ccgtacgatt tcttgttgtc tttaaaagat aatccccttc aaagaagatt tgatttcctt
      601 tttcattggt ccatttgcca tctgcataat ttaataaatc actcatgatc cagccttttt
      661 ctgttttgtc ttgcacaacc atacttgatt ttatggttgt gattttttgt aaaccaaagg
      721 catgggcata aattcctgaa taatctggat atgtatttgg ttctaaatag gtgttgaata
      781 ggcgcattaa agttgtcgga acttctaatc cacaatcatg tgtttcactc aatgccaata
      841 cggcattgta tttaggaaca atgatcagtc ttgaactaaa gaacatactg tttccacctt
      901 ttgccaaaac tccatcacca aaatcatagt ctggatcatg gtgtctgact agatcccagc
      961 ctaagccaaa atcaatggcg cctagatccg attcaagaaa tgtagtgccc caggatttag
     1021 ccattaaagc ttttgacttt tcactaataa tagaattagg ttctagaaag agttgaccaa
     1081 atttgcataa atcaatcata gaagtagtga tgccaccagc tccttgaatt ggaaagtatt
     1141 cttttggttt cttaccttca gaaactaaag gataatctga atggattgta tatgttgtgt
     1201 tggtactttt tgcaccaatc ttttctgtaa tatattttct taagacatct tcataaggca
     1261 tattacgaac ttttgaaacg accatttcag cgagtgtaaa accatcatta caataggtgc
     1321 tataagttcc tggatctgcc tttagagtgc tgtttgataa atagtttaaa acttcgctat
     1381 aatagtcaaa cttagaagtg gtactcacac taaaatgttt ccattgcgta cctggaagtc
     1441 cactcgaatg gtctaggcac atacgtactg taatatcttt atatctttca tctttcattg
     1501 tgaatttagg aagaatatct gcaactttat cttcgagttg aattaatcct tcatccacta
     1561 actgcataac acatacggta caatagattt tagaaatgga acatacatta aatgtacaat
     1621 ccgtcgtgat gggttgattg ttgattttgt cgatgacacc tatcgcatcg gcacaaacaa
     1681 tttttccatc ctgcataaag gcatagctac aagctgtata gtctggtttt accatagctt
     1741 ttaataattg agtaacttga ttttccatat aatttctcct ttttaaaaga aaacgagaag
     1801 acaaaccttc tcgctactct gcaacggtat caattaaaat gctgtgatag gcattcacaa
     1861 tatcatcaac cgtttcttca acattcgcat tgttgacagc tacataagca gcgtattttt
     1921 ggtacaatgg gtatctttct ttatgcattt gttgaagggc ttgtttagaa cttgataaag
     1981 gtctatttgg atcggaacta attaatttat cgatatcacg atcaatgaag atcgtaattc
     2041 catttaaacg taaaaagtcc atattgattt tatgtttaac gacaccacca cctgtcgcaa
     2101 tgattttatt gttcatctta ctcgcttcca tcgcaacttc tgtttcaatc gctctaaatc
     2161 cagcttctcc agattcttgg aagatttctg gaatgctttt gccggctttg gcaataatca
     2221 tatcgtctaa gtcgataaat tcttttccaa gcttttcttc aagcatttta ccaatggttg
     2281 ttttaccagc acttggcata ccaattaata cgatgttaca acgatctttc aacatttctt
     2341 ttttgatttc atcaatgatg gaatcatcaa aagacatatt ttcaaaaatt tcaaatgtat
     2401 atttggcctg cgcaatcaac atctccaaac caactacacg cttgatatcg gcttcttgag
     2461 cttcaaaaca taaacgtgta agaattggat tgtatacaac atccaataca cattctaatt
     2521 tatgaaacca gcttacatca acgggtgcat tcataatatt tggatacatg cctactggac
     2581 ttgtattgac gataatatct gcatctaagt gagatgcata catttcatca tagctaatag
     2641 caccacgatt tgcacttctt gaaacaatca caatgtcttt tgcgttttcg tgttcacaaa
     2701 cggctttaac agcagcgcat gcaccaccgt taccaataat caagactttt tttccttcca
     2761 tatgtacgtt atgtgcttta accatatata agaatccacc aaagtcagtg ttgaatcctt
     2821 ttaatttgcc atctacattg atgattgtat taacagcacc aatagctttg gcactttcat
     2881 ccatttcatc taagtaagga atgacatcct ttttatatgg aatagtcaca ttgattcctt
     2941 tgaaatcttt cttttccata aattctttga attcgtcttt atctaattcc accaattggt
     3001 agtcataatc ttttacgttc tcaatctctg caattcttgt ctgaatttgt ttggagaaac
     3061 tatgccccaa gtgctcacct atcaatccgt atttcatatc atttcctctt ttctagaata
     3121 gcactttaaa tgtgaaaaaa agcatttcga atcagaaatg ctttttgaat gccctgaaac
     3181 ttaaaaataa ccatcgtata catatggctt ttttcaggac tatttaaagt agtagtaata
     3241 ttgtcttgaa ttgttcatat gcataggata caccatttca ttgaaactgg caacccttaa
     3301 a
//
LOCUS       DBMDWL010000201         3279 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13197_length_3279_cov_2.642228, whole genome shotgun sequence.
ACCESSION   DBMDWL010000201 DBMDWL010000000
VERSION     DBMDWL010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13197_length_3279_cov_2.642228"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(172..1908)
                     /locus_tag="ACZPDN_10635"
     CDS             complement(172..1908)
                     /locus_tag="ACZPDN_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1299116.1"
                     /translation="MNGLLLLAIAAALLLTAYLVYGRYLVKTWGIDPKAKTPAYEKED
                     GVDYVPSNKWEVFGHQFSSIAGAGPVTGPVLAMVFGWLPAFLWVMVGGIFFGAAQDFG
                     ALYASVKSHGKSMGQIIETYIGKTGRKLFFLFCWLFTLLVIAAFADMVAGSLNGYNAA
                     GAQSLPSGAASSITILYVFVAIAFGFFLKKTGLSGWKQAVLGIALIVAMLALGIAFPV
                     YFTKQTWVYLVFVYIFFASVTPIWVLKQPRDYLTTFLFIGMIVAAVVGVFVSNPTITS
                     PAFTGFKSATGSYIFPTLFVTVACGAVSGFHSLVSSETSSKQIRNESDMLQVGYGSML
                     LESLLAVLVIVVVGSLTSLASKGVLNETLSSMAFADTATPFIKFSVGVTGLISQFGFP
                     QEWGLCIMTMFVSALALTSLDAVARIGRLSFQELFEVNEEVETNSVVAFLTNKYVATL
                     ITLAGGYLLSLGGYLNIWPLFGSANQLLAAMVLVSLSVFLKVTGRKGYMLYVPMVMML
                     VVTMTSLGMSVYNICLKLFVTGGFAFMTDGLQLFFAVLLMALGLMIFIGSGKKLVQPV
                     EKAKLQEQEQNA"
     misc_binding    complement(1972..2191)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2354..3100
                     /locus_tag="ACZPDN_10640"
     CDS             2354..3100
                     /locus_tag="ACZPDN_10640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1299117.1"
                     /translation="MIYITGDIHREQDIHKINPREFTVGDTLTENDYVIICGDFGCVW
                     DGSTGDNFWLKWLDSLPWNTLFIDGNHENFDVLNTYPVEEYKGGKVHRIRSKVFHLMR
                     GEVFDIDGYKFFTMGGGFSHDVCYRTEHKNWWKDEICTIEEAKHAFETLEKNEWKVDY
                     ILSHDIYSSHPLAHKYEIDMSLYGDNYYDLHDCLETIEKKTEYKMWFSGHYHDDLIHY
                     SDSQKPCLTLFDKVMLLETIDEEMKSLTKI"
     gene            complement(3115..>3279)
                     /gene="ldh"
                     /locus_tag="ACZPDN_10645"
     CDS             complement(3115..>3279)
                     /gene="ldh"
                     /locus_tag="ACZPDN_10645"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014867.6"
                     /note="Converts (S)-lactate and NAD(+) to pyruvate and
                     NADH; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR1299118.1"
                     /translation="YGKEGYFIGTPSVVGSNGVEQVIRLHLNENDQQRFDHSFDTLKQ
                     TIEDNLQDIL"
BASE COUNT         1120 a          543 c          573 g         1043 t
ORIGIN      
        1 aaatgctttt atgacaagcg tactatactg aatatatatt taaatgcaaa acaagagagt
       61 gagtctaaca tggatttcta gttattctga aatggtaaat acaatgtgtt tatgagatag
      121 tgtattctta attgacaaaa gaaaagaccg catgatgcgg tcttgataat tttatgcgtt
      181 ttgttcttgt tcttgaagtt ttgccttttc aacaggttga actaatttct ttccagaacc
      241 aatgaagatc atcaatccta atgccattaa tagaacagcg aagaataatt gtagtccgtc
      301 tgtcataaat gcaaatccac cagttacgaa taactttaag cagatgttgt atacggacat
      361 tcctaagctt gtcattgtta caactaacat cataaccata ggtacatata acatgtatcc
      421 tttacgtcct gtaactttta agaatacaga taatgaaact aataccatgg cagcaagcaa
      481 ttggttagca ctaccgaata atggccagat atttaaatat ccacctaagc ttaataggta
      541 accaccagct aatgtgatta aagtagcaac gtatttgttt gttaagaaag cgacaacact
      601 gtttgtttca acttcttcgt ttacttcaaa taattcttgg aatgataaac gtccgattct
      661 agcaacggcg tctaatgatg ttaatgctaa tgcagaaacg aacattgtca taatacatag
      721 accccattct tgagggaatc caaattgtga aatcaatcca gtaacaccta ctgagaattt
      781 gatgaatggt gttgcagtat cggcaaatgc catagaagat agtgtttcat tcaatacgcc
      841 tttactagct aaactagtta aagatccaac aacaacaatt acaagtacag ctagtaatga
      901 ttcaagtaac atacttccgt atccaacttg taacatatca ctttcgttac ggatttgttt
      961 agaacttgtt tcactagata caagtgagtg gaatccacta acagcaccac aagcaacagt
     1021 gacaaataat gtagggaaga tatagcttcc agttgcagat ttaaatcctg taaatgcagg
     1081 agatgtaatt gttggatttg atacgaatac accaacaaca gcagcaacga tcattccgat
     1141 gaataagaat gtagtcaaat agtcacgtgg ttgttttaat acccagatag gtgtaacact
     1201 cgcaaagaag atataaacga atactagata tacccatgtc tgttttgtga agtaaactgg
     1261 gaatgcgatt cctaatgcta gcatcgcaac gattaatgcg ataccaagta cagcttgttt
     1321 ccacccagaa agacctgttt tctttaagaa gaaaccaaac gcgattgcaa caaatacgta
     1381 tagaatcgta attgaacttg cagctccact aggtaatgat tgtgcaccag ccgcattata
     1441 tccatttaat gatcctgcaa ccatgtcggc aaatgcagca attactaata aggtgaataa
     1501 ccagcagaac aagaagaata atttacgtcc tgttttacca atataagttt caataatttg
     1561 tcccatagat ttaccatgag atttaacaga tgcgtataga gcaccaaagt cttgtgcggc
     1621 tccaaagaag atgcctccga ccattaccca tagaaaagca ggtaaccatc caaataccat
     1681 cgcaagtact ggacctgtta ctggaccggc tccggcaatt gaagagaatt ggtgtccaaa
     1741 tacttcccat ttgttacttg gaacataatc aactccgtct tccttttcat atgcaggtgt
     1801 cttagctttt ggatcgattc cccatgtttt aactaaatag cgtccataaa ctaagtaagc
     1861 tgttagcaat aatgcagcag caatagctaa taataataga ccattcataa taactttcct
     1921 cccttaaaac tttctattca aatattcaaa taataaaaaa agacctcgtc tatctaagac
     1981 gaaatctaat attattccgt ggtaccactt agtttgcaac atctttgttg ccacttagaa
     2041 cttcttaacg cgaagaacga atcattttac tattatttca aatgattagc tcacaaggga
     2101 tagctctaag gaccatcgtt atcatttcac caaatttgat actctctatg accaggtttg
     2161 cttaaagtca tgtcttgttc acagctttga atatttgtgg ttgtataata taccatcttg
     2221 aaaaacatgt caacacaaaa tgtaataaaa tgtaaattga ttttcattaa tttacataaa
     2281 ttacaattgt catttgattg ttaacgtgtg aacgtctgtg ctataatgta aggacgttgt
     2341 tggaggggaa ttcatgattt atataactgg cgacattcat agagaacaag atattcataa
     2401 aatcaatcct agagagttta ctgttggcga tactttaacg gaaaatgatt atgttattat
     2461 ttgtggtgat tttggttgtg tctgggatgg aagtacagga gataactttt ggttgaaatg
     2521 gttagattcg cttccttgga atactttatt tattgatggg aatcatgaaa attttgatgt
     2581 tttaaataca tatcctgtag aagaatataa gggtggtaaa gtccatcgta ttcgttctaa
     2641 agtatttcat ttgatgcgtg gtgaagtttt tgatatagat ggttataaat tctttacaat
     2701 gggcggagga ttttcgcatg atgtatgtta tcgtacagaa cataaaaatt ggtggaaaga
     2761 tgagatttgt acgatagaag aggcaaaaca cgcatttgaa acattggaaa aaaatgaatg
     2821 gaaagtagat tatattttat cgcatgacat atattcatct catccattgg ctcataaata
     2881 tgaaatagat atgagtttat atggagataa ttattatgat ttacatgatt gtttagaaac
     2941 gattgaaaag aaaacggaat ataaaatgtg gtttagtgga cattatcatg atgatttaat
     3001 tcactattca gattcacaaa aaccttgttt gactttattt gataaagtga tgttgttaga
     3061 aactattgat gaagaaatga aaagcctgac taaaatttag tcaggctaat tttattaaag
     3121 gatatcttga agattatctt caattgtttg ttttaatgta tcgaatgagt gatcaaatct
     3181 ttgttgatca ttctcattta agtgtaaacg aataacttgt tctacaccat ttgaaccaac
     3241 aacgcttggt gttccaataa agtatccttc tttaccata
//
LOCUS       DBMDWL010000202         3279 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13198_length_3279_cov_1.897105, whole genome shotgun sequence.
ACCESSION   DBMDWL010000202 DBMDWL010000000
VERSION     DBMDWL010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13198_length_3279_cov_1.897105"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>275
                     /locus_tag="ACZPDN_10650"
     CDS             <1..>275
                     /locus_tag="ACZPDN_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013045.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1299119.1"
                     /translation="KYPAQAIHRIDYETSGLVLFCKHPFFQAYCDKNMEEHHIKKEYI
                     ALVQGHTNYKNKKIISNIARDRHNAKKMIIHPNGKESVSIFNTLKNY"
     assembly_gap    276..285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(427..975)
                     /locus_tag="ACZPDN_10655"
     CDS             complement(427..975)
                     /locus_tag="ACZPDN_10655"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR1299120.1"
                     /translation="MMQILYKLQIVNIIFTLSLYTESMKTLFLMLIITFLIKPFQLST
                     VYFVLFLYFLAKKDYETMYIEKYWIFPSILLLFFCSSYVPLLNRVSTSLVFLFVSGTI
                     KLIKSDWIGSADVCYLVFFGFYLGIERMLVALYISVLLGILWILYKKKHILPYVSCLA
                     IGVWIAVLKGYTIFYFLMNLFS"
     gene            complement(941..2137)
                     /locus_tag="ACZPDN_10660"
     CDS             complement(941..2137)
                     /locus_tag="ACZPDN_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1299121.1"
                     /translation="MQEYRYLLDIALILLMTKAFGLFSRRLRMPSVVGALIAGIVLGP
                     AVLDIVEPSKLIESLSEIGVIVLMFAAGLETSITDLKKAGLKAFIIATLGVLVPLAVG
                     YVVGGFYNVGPDAWLHNLFLGVILTATSVGITVETLKEMGCMSTESGNAILAAAVIDD
                     VLGIICLTLVTGMADSSVNIGVVMFKILLFFVFAVVVGLILHKVFSWWFEHDSCGGLQ
                     RYSIVSFAFALIMAFCSEAFFGVADITGSFVAGLILSGTRQCDYVTKRISTLSYMLIT
                     PVFFASIGLKLSPITWNAKLIELVIVLCVVAVLTKIIGCGLGAIVCKYTPTQAIRIGC
                     GMISRGEVALIVANKGMALGILPEVFVTPVLLCVVFTTVITPILLKIVYKKKPNDADF
                     VQTANC"
     gene            complement(2153..>2841)
                     /locus_tag="ACZPDN_10665"
     CDS             complement(2153..>2841)
                     /locus_tag="ACZPDN_10665"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1299122.1"
                     /translation="LEEKKVETNYISSKLDVIYEDENILIVNKPYGLLSQSDQSQNQD
                     CLVNRVQAYLMEKNEYDPNKEHSFAPSICHRLDRNTTGLVIAAKNANALRKVNEAIAN
                     RTIQKMYRAHVVGTFEQKDFTMCYYIRKENTIAKVSDKKLEGYQKALMDVHVERDDGE
                     NSICTITLHTGRFHQIRALMGHIQHPLLGDVKYGYHGKKRPVELMAYRLNFYDVDLPL
                     QQTVFEIPRA"
     assembly_gap    2842..2851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2852..3051)
                     /locus_tag="ACZPDN_10670"
     CDS             complement(<2852..3051)
                     /locus_tag="ACZPDN_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1299123.1"
                     /translation="MKEIKINQNDANQRLDKFLMKTFPKLGKSMMYKAIRNKKIKVNR
                     KRCTFDQKIQENDSILLFLPPDV"
     gene            complement(3038..>3279)
                     /locus_tag="ACZPDN_10675"
     CDS             complement(3038..>3279)
                     /locus_tag="ACZPDN_10675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024639.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1299124.1"
                     /translation="DIYAKSKVELAHAFKQTCSQDDEIYFVGDSVHDYEVAKSIDAKC
                     ILVTTGHQNRKTLESVNVPVMDSLMESLDFIYERD"
BASE COUNT         1178 a          641 c          424 g         1016 t
ORIGIN      
        1 aaatatccag ctcaagctat ccatcgaatt gactatgaaa caagtggttt ggtgttattt
       61 tgtaagcatc cattttttca ggcctattgc gataaaaaca tggaagagca ccatataaaa
      121 aaagaatata ttgccttagt acaaggtcat acaaattata aaaataaaaa gatcatttca
      181 aatatcgcaa gagatcgtca taatgccaag aaaatgatca ttcatccaaa tggaaaagaa
      241 agtgtttcaa tatttaatac acttaaaaat tataannnnn nnnnnacatc aaatccgtgt
      301 tcaatgtgct tctttgaacc atcccatttt aaacgatcct ctatatggag gtaaaaaaat
      361 tgataataca ggacttttac ttcaaagtca tcatattcaa tttaatgatg tagatattac
      421 ttgtcctcaa gaaaaaagat tcattagaaa atagaatatc gtatatcctt tcaaaacggc
      481 aatccataca ccaattgcca aacacgatac ataaggaagg atatgctttt ttttatacaa
      541 aatccaaagt attccaagta aaacactaat atataaagct acaagcattc tttctatgcc
      601 caaataaaat ccaaaaaaaa ccaaataaca cacatcggca ctgccaatcc aatcggattt
      661 aatcaattta atggttccac tgacaaaaag aaaaactaga cttgtagaga ctcgatttag
      721 taatggcaca taacttgaac aaaagaataa aagaagtatc gatggaaaaa tccaatactt
      781 ttcaatatac atcgtttcat agtctttttt ggcaagaaaa tacaagaata aaacgaaata
      841 aacagtcgat agttgaaatg gtttgattaa aaaagtaatg ataagcatta aaaaaagagt
      901 cttcatactt tcagtataaa gactcaatgt gaaaataata ttaacaattt gcagtttgta
      961 caaaatctgc atcattcggt ttctttttgt atacgatttt taataagatt ggtgtaataa
     1021 ccgttgtgaa tacgacacac aataatactg gagtcacaaa gacctcaggt aaaataccta
     1081 atgccatacc tttatttgcg acaatcaagg caacttcacc acgactgatc ataccacatc
     1141 caatacgaat ggcttgtgta ggtgtatatt tacatacaat agcacctaaa ccacatccga
     1201 taatttttgt caaaacggca accacacata atacaataac taactcaata agtttggcat
     1261 tccatgtgat tggagaaagt ttcaatccaa tactcgcaaa gaatacaggt gtaatcaaca
     1321 tataagataa tgttgaaata cgttttgtga cataatcaca ttgtctagta ccagaaagaa
     1381 tcaaaccagc cacaaaacta ccagtgatat ctgcaacacc aaagaatgct tctgaacaga
     1441 aggccatgat caatgcgaac gcaaaagaca caattgaata tctttgtagt ccaccacatg
     1501 aatcatgttc aaaccaccat gagaatactt tgtgtaaaat caaacctaca acaacggcaa
     1561 acacaaagaa tagtaaaatt ttaaacataa caacaccaat attaactgaa ctatcagcca
     1621 tacctgttac taatgttaaa caaataatac ctaaaacatc atcaataaca gcggctgcta
     1681 gaatcgcatt tccactttct gtagacatac atcccatttc ttttaatgtt tctactgtaa
     1741 taccaacact tgtagctgtt aaaataacac ctaagaataa attgtgtaac catgcatctg
     1801 gtccaacatt atagaatcca ccaaccacgt atccaacagc taatggaacc aacacaccaa
     1861 gtgtcgcaat aataaatgct tttagtcctg ctttctttaa atctgtaatg cttgtttcaa
     1921 gaccagccgc aaacattaag acgataacac caatttccga taaagattca attaacttac
     1981 ttggctcaac tatatctaat accgcaggtc ctaaaacaat acctgcgatc aatgcaccta
     2041 ctacagatgg catacgtaat cgacgagaaa aaagtccaaa ggcttttgtc attaataaaa
     2101 ttaaggcaat atctaataga tatcgatatt cttgcatatt cataccctcc tactatgctc
     2161 ttggaatttc aaacaccgtt tgttgaagtg gtaaatctac atcataaaaa tttaaccgat
     2221 atgccatcaa ttctaccggc cttttctttc catgatatcc atattttaca tcacccaaca
     2281 aaggatgttg tatgtgcccc attaaagcac gaatctgatg aaatcttcct gtatgaagag
     2341 taattgtaca aattgaattc tcgccatcat ctctttcaac atgtacatcc attaatgctt
     2401 tttgatagcc ttctaatttt ttatcactta cctttgcaat tgtattttct ttgcgaatgt
     2461 aataacacat agtaaaatct ttttgttcaa aagtacccac aacatgggct cgatacatct
     2521 tttgaatcgt acgatttgcg attgcctcat ttacttttct taaagcattc gcattttttg
     2581 cagcaatcac tagacccgtt gtatttcgat ctaatctatg acaaatacta ggtgcaaaag
     2641 aatgttcctt attaggatca tattcgtttt tctccattaa atatgcctgc acacgattca
     2701 ctagacaatc ttgattttga ctttgatcac tctgtgataa taagccatat ggcttattca
     2761 caattaagat attttcatct tcataaatga catctaattt cgatgatata taatttgttt
     2821 ccactttttt ctcttctaaa annnnnnnnn nacatccgga ggtaaaaaca atagaatact
     2881 atcattttcc tggattttct gatcaaaagt acaacgcttg cgatttactt tgattttttt
     2941 atttcgaatc gccttataca tcattgactt gcctagctta ggaaaagttt tcattaaaaa
     3001 cttatctaat ctttgattcg catcattttg attgatctta atctctttca taaataaaat
     3061 ccaaactttc cattaaactg tccataaccg gtacattcac actttctaaa gtcttacgat
     3121 tttgatgccc tgttgtcacc aagatacatt tggcatcaat actttttgcg acttcataat
     3181 catgaacgct atctcctaca aaataaattt catcatcttg gctgcatgtt tgtttaaacg
     3241 catgggctaa ttccacttta cttttcgcat agatatcat
//
LOCUS       DBMDWL010000203         3265 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13281_length_3265_cov_4.241466, whole genome shotgun sequence.
ACCESSION   DBMDWL010000203 DBMDWL010000000
VERSION     DBMDWL010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13281_length_3265_cov_4.241466"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(197..1762)
                     /locus_tag="ACZPDN_10680"
     CDS             complement(197..1762)
                     /locus_tag="ACZPDN_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299125.1"
                     /translation="MKSLIFFIPEFRSSTPLAEGIKDIANKYNLEFELVTIKKDVNPL
                     QQQQFLYAVHVSDIVVVDCTIPSDVNDGGVYPALTAQVNILNHIIVVSENNLPLNITP
                     LRGVFPQKDGERCTIEHILKKLPEQIKQSLKEDTYSRIPDDMINDFLKYQKEMEEMLG
                     ASLVIRRRKKYEKTSVMISYRNSHSEEVEKFKSIITGTDIQSKKERHRLGCDGDYELK
                     VLPPASLCGADEAHTPMRRWMLVGLLEDHIREVDEVWVYESRNAKGEIDYTKSWWTIA
                     EMIMVANINYDNTKKIKVKVYNPIKKQFYETTQQQYLVNITEAQHKRLARYLSNTRPD
                     TMGPECLAQVKQLKMIANLMRHSTKDMKQQMLENLRANFELSVPKTLEEAERKQMIDD
                     MIKMYSDPDEIERYANDDVFQEKFWNDISYQTAEATDCFTNNRIDVDKFMSTPMDEVT
                     GHPLEDFVKASLENEDTINLGTSENPLKYKVSKANIDRYLWLATRMGRPTVKEGNAPG
                     LERIPLYNITRCE"
     gene            complement(1759..2595)
                     /locus_tag="ACZPDN_10685"
     CDS             complement(1759..2595)
                     /locus_tag="ACZPDN_10685"
                     /inference="COORDINATES: protein motif:HMM:NF025056.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR1299126.1"
                     /translation="MKYDVFISYKRDGGSVWAELIRAILVHKYHLKVFLDVETVRGGE
                     WPKQLDDGIRNSYNIIMVLFEGIGDKIKSDSDVFVQEIEHAKEYEKPIIPFYGLGCDL
                     LYILENKNIPSIIKEVVSIQHSIVKYDHANSEKTYDLLRKQLNGNLELKVTSKYSPCY
                     MSCQLNNEPPYETKEIEENSNLSICLDRNFTGIVHLRFYTKELPIKIERIINVGEKSY
                     IEENQSVNCEYWHWQPDKELTKEVPLDWQLIKIDCQINQSYNPLQAVLGTFNQYLNKE
                     SV"
     gene            2988..>3265
                     /locus_tag="ACZPDN_10690"
     CDS             2988..>3265
                     /locus_tag="ACZPDN_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299127.1"
                     /translation="MHIIAKNIEENVNASFIKSEISTETFKKMPCNSKSFSKITICFN
                     RLRKYMYPFFEKHHLVKNNTMNGIIKKTGIFANPLEKATKLFSNLCNKS"
BASE COUNT          985 a          583 c          515 g         1182 t
ORIGIN      
        1 cgactcaatc attagagagt ttgtgacagt gatgaaaata agcaaaatca agtttatggt
       61 ttattaacga gaagttttct ataggggtgc tgagtagtta cgaataccct cgtgttgtca
      121 gtctatagta taaattgttt gcagtagcaa gactcttgcg tttataactg atgaatatat
      181 cgtatgtcat agaaatttat tcgcatcttg ttatattgta tagcggtatt ctttctagtc
      241 caggagcatt tccttctttc actgttggtc tacccatacg agtagctaac cagagatacc
      301 tgtctatatt tgcttttgat accttatatt tcaaaggatt ttccgaagta ccaagattga
      361 tggtatcttc attttcaaga gatgctttca cgaaatcttc taatggatgt ccagtcactt
      421 catccatcgg tgtagacatg aatttgtcaa catctatgcg attgtttgta aagcaatcag
      481 tagcttccgc tgtttgataa gatatgtcgt tccaaaactt ttcttggaaa acatcgtcat
      541 tggcataacg ctctatttcg tcagggtcag aatacatttt aatcatatcg tcaatcattt
      601 gcttgcgttc agcttcctct aatgttttgg ggacgcttaa ctcaaagttg gctcgaaggt
      661 tttcaagcat ttgttgcttc atgtcctttg tactgtgtcg cataagattg gctatcatct
      721 tcaattgttt cacctgtgcc agacattctg gtcccatagt gtcaggacga gtgttggaaa
      781 ggtatcttgc cagacgctta tgttgagcct ctgttatatt gacgaggtat tgttgttgtg
      841 tcgtctcata gaattgtttc ttaatcggat tataaacttt caccttgatt ttcttggtgt
      901 tatcataatt gatatttgcc accattatca tttctgctat tgtccaccaa gatttagtat
      961 agtctatttc tcctttagca ttacgactct catacaccca tacctcatcg acctctcgaa
     1021 tatgatcttc caataaacca acaagcatcc atctgcgcat aggggtatga gcttcatctg
     1081 caccacataa tgaagcagga ggtaaaacct tcagttcata gtcaccatca catccaagtc
     1141 tatgacgttc tttttttgat tgaatatcag tacctgtaat tatagattta aacttctcaa
     1201 cttcttcact gtgactgttg cgataactaa tcattacaga agttttttcg tatttcttcc
     1261 ttctgcgtat tactaatgaa gccccgagca tttcttccat ttccttttgg tattttagga
     1321 aatcgttaat catatcatct ggaatgcgac tgtatgtgtc ttctttcaga gattgcttaa
     1381 tttgttctgg taactttttc aagatatgct cgatagtaca tctttcccca tctttttgag
     1441 ggaaaacacc tcgtaaaggt gtaatgttta atggcagatt attctcgctc acaacaatta
     1501 tgtgattgag aatatttact tgtgctgtca atgcaggata aacgccaccg tcatttacgt
     1561 ctgatggtat tgtacaatct actacgacaa tatctgatac atgtactgca taaagaaatt
     1621 gttgctgttg taatggatta acatctttct ttatagttac gagttcaaac tctaaattat
     1681 atttattagc aatatcctta ataccttctg ctaaaggagt actgctgcgg aattccggga
     1741 tgaaaaatat taatgatttc atacgctttc tttattaaga tattgattga atgtacctaa
     1801 tactgcttgc aaaggattgt atgactgatt aatttgacaa tcaatcttta taagctgcca
     1861 atcaagagga acttcttttg ttagttcttt gtcaggttgc caatgccaat actcacaatt
     1921 gacactctga ttttcttcaa tatatgactt ttctcctacg tttataatgc gctcaatctt
     1981 tattggaagt tcttttgtgt agaaccttaa atgaacaata cctgtgaaat tcctatctaa
     2041 acagatggaa agattgctat tttcttcgat ttcttttgtt tcataaggtg gttcgttgtt
     2101 taactgacaa gacatatagc acgggctata cttgctggtt actttcaatt ctaaattacc
     2161 attgagttgc ttacgtaata aatcatatgt tttctcgcta tttgcgtgat cgtatttaac
     2221 aatgctatgt tgtattgaca cgacttcctt tataatagac gggatatttt tgttttccaa
     2281 aatgtatagc aaatcgcaac caagaccata aaagggaata attggctttt cgtattcttt
     2341 ggcatgttct atttcttgca caaaaacatc actgtccgat tttatcttgt caccaatacc
     2401 ttcaaataaa accattatta tgttgtaaga gtttctgata ccatcatcca attgttttgg
     2461 ccattcacct ccacgcacag tttcaacatc taaaaacact ttgagatggt acttgtgtac
     2521 taaaatagct cgtataagtt ccgcccatac actaccacca tcacgtttat aacttataaa
     2581 tacatcgtat ttcattggct ttctttatgt ttcgttgaga acaaatgaac agaaattaat
     2641 attttatttc atgtaatatc cttatgaagt gactatgtat attaaacatg actttgtttt
     2701 ttaagatcaa tttccgtgtt tgaaaagtat tgtatgaaaa attatcccta taagactgaa
     2761 cccatccaat agaaaatgat aataacaaca actttcccca attgttagga ttgaaaatat
     2821 ctccatttat aatatttatt gtattataag cagataagac taacaaaaaa gctatcatca
     2881 acatcaatat tgtgcgaata ttaaaagtta aagtgttgca gataatctct tttgtaacat
     2941 ttgtataata aagctcatga aaaaacttgt gtataataat tattggtatg catattattg
     3001 ccaaaaacat tgaagaaaat gttaatgctt catttataaa gtctgagata tctacagaaa
     3061 cattcaaaaa gatgccttgc aatagtaaat ctttttcaaa aataactata tgttttaata
     3121 ggctaagaaa atatatgtat ccattctttg aaaaacacca cttggtaaaa aataacacca
     3181 tgaatggaat tataaagaaa accggaattt ttgctaatcc attagaaaaa gcgactaaac
     3241 ttttttcaaa tctttgtaat aaatc
//
LOCUS       DBMDWL010000204         3244 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13409_length_3244_cov_2.515431, whole genome shotgun sequence.
ACCESSION   DBMDWL010000204 DBMDWL010000000
VERSION     DBMDWL010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13409_length_3244_cov_2.515431"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="ACZPDN_10695"
     CDS             <1..573
                     /locus_tag="ACZPDN_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1299128.1"
                     /translation="DFYNVMDAFHSFNPDLVLMDITLPYLNGYLWTEKIREESKVPII
                     FISSATDNMSIVMAISKGADDFVCKPFDLNVLAAKITAILRRSYDFVSNHNILEYQGV
                     RFNLDDNTVSFQSSQMELSKNEAKILRLLMEKKGTIVSRDCLMEYLWKTDFYVDENAL
                     SVNVNRLRKKLESIGIVDFIQTKKGLGYKI"
     gene            570..1559
                     /locus_tag="ACZPDN_10700"
     CDS             570..1559
                     /locus_tag="ACZPDN_10700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1299129.1"
                     /translation="MKLFGHYLKDRLFFIMIYVVSVLICLCVFKLYNLELEAFTYVFM
                     ILCVLLVVCFLFDYYKYYKKHQTLVTLQSNSNVSLSCDLQDSSLMGEDYHKILVAMKE
                     YHDEYVESSESKMHDLEDYFTMWVHQAKLPIAAMKLLLEDENLSRNEIKLQLLRMDQY
                     TDMVLAYLRLHSTHTDFLFKELELDDLIRQSIRRFSTEFIRKHIQLSFKETGDVILSD
                     EKWLVFVLEQILSNSLKYTNENGLISIYMKSKHVLVIEDNGIGISASDLSRVFEKGYT
                     GMNGRSDKTTSGLGLYLCKNILDMLNHKIRIESSVGKGTRVILDLTHFEGRIE"
     gene            1651..2418
                     /locus_tag="ACZPDN_10705"
     CDS             1651..2418
                     /locus_tag="ACZPDN_10705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1299130.1"
                     /translation="MALLEVKNLKKVYTTRFTQNQVQALTNVNFSVNKGEYVAIMGES
                     GSGKTTLLNILAALDKPTSGQVLLHGKDITHISEKEIANFRREHLGFVFQDFNLLDNF
                     NNRDNIFLPLVLSKKKYPEMKKRLEPIAKQLGIEGILDKFPYEISGGQKQRVAVARAI
                     ITNPDLILADEPTGALDSKSTEELLHLFGTINENQQTIVMVTHSTKAASHASRVLFIK
                     DGKIFHEIYKGNKNLDQMYQCISDTLTLLATGGVHHE"
     gene            2411..>3244
                     /locus_tag="ACZPDN_10710"
     CDS             2411..>3244
                     /locus_tag="ACZPDN_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1299131.1"
                     /translation="MSRAFYLNLAVDNLKKNARTIIPYILTSVLTTMMLYMVVSLANN
                     PNLNEMLGAMTLTQMLGFGVVIIEIFAFIFLFYTHSFLIKRRQKEFALFSILGMEKKH
                     LARVLFYETAITLFVSLALGIGLGILFDKAMFLIIAKMIGADIILGFYFSFIGMRQCV
                     LVIGLIYVLIYFYSMIRIHISSPIELLHSSHMGEKEPKAKWFLSIVGILCLGIGYYLS
                     ITTKNPLTALYFFFVAVVLVIVGTYLLFTSISVTFLKLMKKNKNYYYKTNHFISVSGM
                     MY"
BASE COUNT         1045 a          411 c          637 g         1151 t
ORIGIN      
        1 gatttttata atgtgatgga tgcatttcat tcctttaatc cagacttagt attaatggat
       61 ataacactgc cttatttgaa tggatactta tggacggaaa agattcgtga agaatccaaa
      121 gttccaatta tctttatttc aagtgctaca gacaatatga gtattgtgat ggcaatctct
      181 aagggtgcag atgattttgt gtgtaaacca tttgatttaa atgttctggc tgctaaaatt
      241 acggcaatat taagacgtag ctatgacttt gtatccaatc ataatatcct tgaatatcaa
      301 ggtgtacgct ttaatttaga tgataataca gtaagttttc aatcttctca aatggaactg
      361 agcaagaatg aggcaaagat cttacgttta cttatggaaa aaaaggggac gattgtatct
      421 cgagattgtt taatggaata tttgtggaag acagattttt atgtggatga aaatgcatta
      481 agtgtgaatg tgaatcgttt gcgtaaaaag ttagagagta ttggaattgt ggattttatt
      541 caaacaaaaa aagggttggg atataagata tgaaattatt tggccattat ttaaaagatc
      601 gattgttttt tataatgatt tatgttgtaa gtgttttgat ctgtttatgt gtatttaaac
      661 tctataatct agaacttgaa gcttttacat atgtctttat gattttatgt gttttgttgg
      721 tggtttgttt cttatttgat tactacaaat attataagaa acaccaaacc ttagtaacac
      781 ttcaatccaa tagtaatgtt tctttgtctt gtgatcttca agattcttca ttgatgggag
      841 aagactatca taaaatctta gtggctatga aagaatatca tgatgaatat gttgaatctt
      901 cagaaagtaa aatgcatgat ttagaagatt attttacgat gtgggtgcat caggctaagc
      961 ttcctattgc cgctatgaaa ttactacttg aagatgagaa cttaagtcgt aatgaaatta
     1021 agttgcaact cttacgtatg gaccaatata cagatatggt tttagcctat ttacgcttac
     1081 atagtacgca taccgatttc ttatttaaag aattagaatt ggatgattta attcgacaat
     1141 cgattcgtcg tttttctaca gaatttattc gtaaacacat tcagttgagc tttaaagaaa
     1201 ccggggatgt tattttgagt gatgaaaagt ggcttgtgtt tgtgttggaa caaattcttt
     1261 cgaatagtct taaatatacc aatgaaaatg gtttgatatc tatatatatg aagtctaaac
     1321 atgttttagt cattgaagat aatggtatag gtattagtgc aagtgatcta agtcgtgtat
     1381 ttgaaaaggg atatacaggt atgaatgggc gtagtgataa aaccacaagt ggtctaggtc
     1441 tatatttgtg taagaatatt ttagatatgt tgaatcataa aatcagaatt gaatcaagtg
     1501 ttggtaaagg cactcgagtg atattggatc ttacgcattt tgaaggaaga atagagtaat
     1561 cttacaatct tgtaaggata gtgttagctg aatcgatggc tgtatttttg gattctttgt
     1621 agaattttag tgtacatgga ggtaatggat atggcattat tagaagtaaa aaatttaaaa
     1681 aaagtatata caacaagatt tacacagaat caagtacagg cattgaccaa cgttaatttt
     1741 agtgtgaata aaggcgaata tgtagctatc atgggtgagt ctggttcggg taagacaacc
     1801 ttattaaata tcttggcggc tttagataaa ccaacaagtg gacaagtctt actacatggt
     1861 aaagatatta cgcatatttc agaaaaggaa attgcgaact ttcgaagaga acatttaggc
     1921 tttgtattcc aagattttaa tttattagat aattttaata atcgcgataa tattttcttg
     1981 cctttggttt taagtaagaa aaaatatcca gaaatgaaaa aaagattgga acctatcgca
     2041 aaacaattag ggattgaggg tattttagat aagtttccat atgaaatcag tggtggacaa
     2101 aagcagcgtg tagctgttgc tcgtgccatc atcacaaatc cagatctaat tttggcggat
     2161 gagccaacgg gggctttgga ttcaaagagt acagaggaac ttttacattt gtttggaacc
     2221 atcaatgaaa atcaacagac gatcgtaatg gttactcatt cgacaaaggc tgccagtcat
     2281 gcttctcgcg ttctatttat taaggatgga aagatcttcc atgaaattta taagggaaac
     2341 aagaatttag atcagatgta ccaatgtatt agtgatacat tgacactact agctacaggt
     2401 ggtgtgcatc atgagtagag ctttctattt aaatttagct gtagataatt tgaaaaagaa
     2461 tgcgcgtacc attattcctt atatactaac gagtgtctta acgacaatga tgttgtatat
     2521 ggttgtttct ttggcaaaca atccaaactt aaatgagatg ttaggggcaa tgacactaac
     2581 gcagatgtta ggctttgggg ttgtcattat tgaaatcttt gcgtttatct tcttgttcta
     2641 tacacatagc tttttaatta aaagaagaca aaaagagttc gcgttattct ctatattagg
     2701 aatggaaaag aaacacttag ctcgcgtctt gttttatgaa actgcgatta ctttatttgt
     2761 atcattagct ttaggtattg gtttaggtat cttgtttgat aaagcgatgt ttttaatcat
     2821 cgcaaaaatg attggtgcag atattatact tggtttctat ttctcattta taggtatgcg
     2881 acaatgcgtt cttgtgattg gattgattta tgtattgatc tatttctatt ctatgattcg
     2941 tattcatatt tcaagtccaa ttgaattgct tcattcgagt catatgggtg aaaaagagcc
     3001 taaggccaaa tggtttttat ctattgttgg tattctttgt ctaggtattg gatactattt
     3061 gtcaattacg actaaaaatc ctttaacggc actctatttc ttctttgtgg ctgttgtact
     3121 tgtcattgtt ggaacgtatt tattatttac atccatatca gtgactttct taaaattaat
     3181 gaagaaaaac aagaattact attataagac gaatcacttt attagtgttt ctggcatgat
     3241 gtat
//
LOCUS       DBMDWL010000205         3227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13520_length_3227_cov_2.284389, whole genome shotgun sequence.
ACCESSION   DBMDWL010000205 DBMDWL010000000
VERSION     DBMDWL010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13520_length_3227_cov_2.284389"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            258..998
                     /locus_tag="ACZPDN_10715"
     CDS             258..998
                     /locus_tag="ACZPDN_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR1299132.1"
                     /translation="MNDLNLGINELELGDKLVRNGQHTSIVTIASSGPRFIKANMFPR
                     DNTVKLGDPISLTVGYRGGLSSRCGFAVENETQLPVDQKDYLERITIPYFNAYVAWLE
                     NIKCGLQGHEIFDLIESELPKEKYGWSLCPGHLTAEEEWMSSPIYENSTEVLQSGMLL
                     QIDIIPSVSGYTGSSAESTILLADENLRNQIKKEYPGMYNRMTERRTYIINTLGINLS
                     EDVMPMCSTVAYLRPFLLAHDKALVVKQ"
     gene            1305..1775
                     /locus_tag="ACZPDN_10720"
     CDS             1305..1775
                     /locus_tag="ACZPDN_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1299133.1"
                     /translation="MFGFLKKKEVFLCPVDGKILPIGEVNDPVFSQEMMGPGFAVVPT
                     SKVVVAPMDGEVVTVFPTSHAFGIKSKNGIEMIIHIGIDTVELNGKGFKPLVKQGDVV
                     KAGDALVEVDFDVLKSEGKDPTCMIIFPNKEKFSIIKSHSNVTTGQDNIIEFNK"
     gene            1943..>3227
                     /locus_tag="ACZPDN_10725"
     CDS             1943..>3227
                     /locus_tag="ACZPDN_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HIR1299134.1"
                     /translation="MKVDVKRIQNDIETLATFNETPGCGVTRSSYTKEDKMAKEYLIS
                     EMEKIGLTVYEDGMSTLFGRREGKLKDAPCVMLGSHYDTVYHAGAFDGVAGSVSALEV
                     MRVLHENNFENDYPLELILMNAEEGFTFGPSTGVTNSRAMMGTLTYHELETRKNRFGQ
                     TKLEALRAYGVEPDLEKAKRDPKTIKNFLELHVEQGPVLDRENIEVGLIKYLAGIGRY
                     TIKFHGKTADSTELMSKRKDALVGAAYFIEKFNAVMRTFGDDATGMVGQLNIVPNSNQ
                     FVPEYVEGKIEIRTFTKEVVDNNDFNKIITDLLNEVTERYGIQTELEEIRRVNYPNPT
                     GPSIMNYENVEKMEKICDELGYSHKQMNQGTGHDSMIMADFVDTNMIYVPSRNGGVSH
                     CPEEWTEYTDIEKGANVLLELVKDLSMSVYSVHSSG"
BASE COUNT         1175 a          403 c          602 g         1047 t
ORIGIN      
        1 attggtttag taggctggaa aaacttcact tctattttag aggataacaa acacacatat
       61 gatgttccat attatattgt taaaaatatt attatattgt taaaaatatt attgatatta
      121 ttggcgatga atacttatta agtaatgaat gttcaatatt tattggtgaa aatggcgtca
      181 gatgtacaaa taatccaaat gaaatagctc attatgaata tggtgcagca ttagctagtg
      241 attgtatttt agatgccatg aatgatttaa atcttggcat aaatgaacta gaactaggtg
      301 ataaacttgt tagaaatgga caacatactt ctattgttac aatagcttct agtggtccta
      361 gatttattaa agcaaatatg tttccacgtg ataatactgt taaattaggt gatcctattt
      421 ctttaacggt aggataccgt ggtggcttaa gttcaagatg cggatttgct gtcgaaaatg
      481 aaacacaact tcccgttgat caaaaagatt atttagaaag aattactatt ccatatttta
      541 atgcttatgt tgcctggctt gaaaacatca aatgtgggtt acaaggtcat gaaatatttg
      601 atttaattga aagtgaatta ccaaaagaaa aatatggttg gtcattatgt cctggtcatt
      661 taactgctga agaagaatgg atgtcttctc ctatttatga aaactcaact gaagtattac
      721 aatctggaat gctattacaa atcgatatta ttccatcagt ttctggttat actggttctt
      781 ctgctgaaag tacaattctt ttagctgatg aaaatttacg taatcaaatc aaaaaagaat
      841 atccaggtat gtataataga atgactgaaa gaagaactta tataattaat actttaggta
      901 tcaatctatc agaagatgtt atgcctatgt gctctactgt cgcttattta agaccgttct
      961 tactagctca tgataaagca ttggttgtaa aacaataaat aatctttaat cacgaaaatc
     1021 caaagattat ttcatgtgta atcaccataa gctcaattgt aaaactaaac cctattaaga
     1081 aatagtaaat accacgaggt tgaatttagt aattccaaat ttactgaaat tcaaccattt
     1141 tttgaattct gcccattgca attagaattt ttaaaagata gtaacaataa taaagataag
     1201 acttatgatt ttataggata ataaaattaa agaatagata ttcataaaat gaatgataaa
     1261 tcgaaagaat ccatactata attagtaaaa acggaggaat aaatatgttt ggctttttaa
     1321 agaaaaaaga agtgttctta tgtccggtag acggaaaaat tttaccaatt ggtgaagtga
     1381 atgatcctgt gttttcacaa gaaatgatgg gtccaggatt tgcggtggtt ccaacatcaa
     1441 aagttgttgt tgcgccaatg gatggagaag ttgtaacggt atttcctact tcacatgcct
     1501 ttggtattaa gtcaaagaac ggtattgaaa tgattattca tattggtatt gatacagttg
     1561 aattaaatgg aaaaggcttt aaacctttag taaaacaagg ggatgttgtt aaagcaggag
     1621 atgctttagt agaggttgat tttgatgtat tgaagagtga aggtaaagac ccaacttgca
     1681 tgattatatt ccctaataaa gagaaatttt caattattaa atctcacagt aatgtaacaa
     1741 ctggccaaga taatattatt gagtttaata aatagataaa atcaaaaaga gtaggagatt
     1801 ctttgtaatt tttcctgctt ttttaaataa atattcagaa tttgaatata caaccataat
     1861 gatttagatg cattgttgct ttaatttttc aatgatatga tttggttgct taaaggagta
     1921 ttagattaaa ggagtattag atatgaaagt agatgtaaaa agaattcaaa atgatattga
     1981 gactttagcc acatttaatg aaactcctgg ttgtggtgta actagatctt cgtacacaaa
     2041 agaagataaa atggctaagg aatatttaat atctgaaatg gaaaaaattg gattgactgt
     2101 ttatgaagat ggcatgtcaa cattatttgg acgtcgtgaa ggtaaattaa aagatgcgcc
     2161 atgtgttatg ttaggatctc attatgacac agtttatcat gcaggcgcat tcgatggagt
     2221 tgccggatcc gtttcggcat tagaagttat gcgtgtatta cacgaaaata attttgaaaa
     2281 tgattatcct cttgaattga ttttaatgaa tgctgaagaa ggatttactt ttgggccaag
     2341 tactggtgtt acaaactcaa gagctatgat gggaactctt acttatcatg agctagagac
     2401 tcgtaaaaac cgttttggac aaactaaatt agaggcttta agagcgtatg gtgttgaacc
     2461 agatttggaa aaggcaaaaa gagatccaaa aacaattaaa aacttcttag aattacacgt
     2521 tgaacaaggt cctgttttgg atcgtgaaaa tattgaagtt ggattaatta aatatcttgc
     2581 tggtattgga agatatacaa tcaaattcca tggaaaaact gcagattcaa cagaattaat
     2641 gagtaaaaga aaagatgctc tagtaggagc tgcttatttc attgaaaaat tcaatgcggt
     2701 aatgcgtaca ttcggtgatg atgctacagg aatggttgga caattgaata ttgttcctaa
     2761 ctcaaatcag ttcgtaccag aatatgttga gggtaaaatt gaaattcgta ctttcacgaa
     2821 agaagtagtt gataataatg atttcaataa gattattaca gatttattaa atgaagtaac
     2881 agaaagatat ggtattcaaa ccgagttaga agaaattcga agagtcaatt atccaaaccc
     2941 aacaggtcct agtattatga attatgagaa tgttgaaaaa atggaaaaaa tttgtgatga
     3001 actaggctat agtcataaac aaatgaacca aggaacagga catgattcta tgattatggc
     3061 tgattttgta gatacaaaca tgatttatgt acctagtaga aatggtggag tatctcactg
     3121 tccagaagaa tggacggagt atacagacat tgaaaaaggt gctaacgtat tacttgaatt
     3181 agttaaagat ttatcaatgt ctgtatactc cgtccattct tctggac
//
LOCUS       DBMDWL010000206         3192 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13724_length_3192_cov_3.555160, whole genome shotgun sequence.
ACCESSION   DBMDWL010000206 DBMDWL010000000
VERSION     DBMDWL010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13724_length_3192_cov_3.555160"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(318..394)
                     /locus_tag="ACZPDN_10730"
     tRNA            complement(318..394)
                     /locus_tag="ACZPDN_10730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Arg,seq:cct)
     gene            complement(406..1032)
                     /locus_tag="ACZPDN_10735"
     CDS             complement(406..1032)
                     /locus_tag="ACZPDN_10735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1299135.1"
                     /translation="MKTILFDLDDTLIESMHVWEDAVIRLFKKMDLYMSMEEARQIFF
                     TMKFSEVLTYIKERFHTKQDEAWMFNEVNEDILNQYAYHIEAKKGALDYIKKCASENK
                     KMAVLTSNTRALSEKVLDRLGMLEYMENVYSADELKMSKREVDIYEYVLKDLNTTAES
                     AIVFEDSMYAINTARSLNIECIGLVNPNNQKVFEKNHVKTIVDFTELC"
     gene            complement(1045..1121)
                     /locus_tag="ACZPDN_10740"
     tRNA            complement(1045..1121)
                     /locus_tag="ACZPDN_10740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1085..1087),aa:Arg,seq:ccg)
     gene            1213..2466
                     /gene="uraA"
                     /locus_tag="ACZPDN_10745"
     CDS             1213..2466
                     /gene="uraA"
                     /locus_tag="ACZPDN_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HIR1299136.1"
                     /translation="MAKRIIQVEEKVPANLLIPLSIQHMFAMFGASVLVPFLFGINPA
                     IVLFMNGIGTLLFIFITKGKAPAYLGSSFAFIAPANIIIAKFGYPYACGGFVAVGFCG
                     CLLAMIIKKFGTKWIDIVLPSAAMGPVVALIGLELSSSAANTAGILGDNIDPKNVIVF
                     AVTLGMAVIGSVCFKKFLSVIPILIAVVTGYLTAVVVGIVDFTPVLQASFISIPNFQA
                     PKFSMDAILMMLPVLLVIASEHIGHQIVTGEVVGRNLIEDPGLHRSLFADNFSTMISG
                     LIGSVPTTTYGENIGVMAITGVYSVQVIAGAAILSIICSFIGPLSALIQTIPNPVIGG
                     ISFLLYGMIGTSGLRILVDQKVDYGKSVNMILTSVVFVVGLSGVTIHLGNISLSGMVL
                     ACITGMIMSLLFYIFEKFHLLNEEV"
     gene            complement(2500..3045)
                     /locus_tag="ACZPDN_10750"
     CDS             complement(2500..3045)
                     /locus_tag="ACZPDN_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR1299137.1"
                     /translation="MKVAVLLENGFEELEAMGPIALLRRGGLDVDMIGVNNEEQVTGR
                     FGITYSSVYPMNTYDFSNVDCLVLPGGPHYQKLEKNEEVLKLAHEFAENKVLAAICAS
                     PTILGHEGILKGKKYTCFKDMDEDFGGEYQYEYAVTDGNIITGVSAAASIEFAFAILE
                     KLCGKEHTEKVKASIYYDAKH"
BASE COUNT         1012 a          638 c          485 g         1057 t
ORIGIN      
        1 cgagtccaat atcagctata aaccgttttg gggtatatgc cgccccttaa attcttggag
       61 agcaaattca cctaacccca tccaaattta ttgcgccttt tgcttgcaca attaatgcta
      121 ttgaaaagaa caagaagaaa taacccccaa tttttctaca agaattcata caaagaaatt
      181 aacttggtac gaaaacattt cccattcctc acttatcaca aaacaaaaag agatttgaat
      241 gatgaacaca tcagacaaat cccttttatc atttacaggc taaaatccat taaaaatcct
      301 gttaaaactc aaaaaaatgg cgtcccagat aggagtcgaa cctacgacct ttcgcttagg
      361 aggcgaatgc tctatccaac tgagctactg ggacacatca tgattttaac acaattctgt
      421 aaaatcaacg attgttttaa catggttttt ttcaaacacc ttttgattgt ttggatttac
      481 taaaccaata cactcaatat ttaatgatcg agctgtattg atcgcataca tcgaatcctc
      541 aaatacaatc gcactttctg cagttgtatt taaatctttc aatacatact catagatatc
      601 cacttctctt tttgacatct tcaactcatc tgcagaatat acattttcca tatattccaa
      661 cataccaagt cgatctaata ctttttcaga taatgctctt gtgtttgatg tcaatacagc
      721 catcttttta ttttctgaag cacacttctt tatataatcc aaagcaccct tctttgcctc
      781 aatatgatac gcatattgat tcaagatatc ttcatttact tcattgaaca tccaagcctc
      841 atcttgttta gtatgaaatc tttctttaat gtatgttaaa acttccgaaa atttcattgt
      901 aaaaaaaatt tgacgagcct cttccatact catatacaaa tccatctttt taaataaacg
      961 aatgacggca tcttcccata cgtgcatcga ttcaattaat gtatcatcta aatcaaaaag
     1021 tattgttttc atgtttcacc taaatggtgc gcctgacagg tctcgaacct gcaaccgcca
     1081 gaatcggaat ctgatactct atccagttga gctacaggcg cactacctaa taattatagc
     1141 ctaaattagt ttcataatcc attaaaatgt ggtataaaag ttaaggtata ttttaggagg
     1201 agaatatttt tcatggcaaa acgaattatt caagttgaag aaaaagtacc cgcaaatctg
     1261 ctgataccat taagtattca acacatgttc gcaatgtttg gagccagtgt attagtacca
     1321 ttcttattcg gtattaaccc agctatcgta ctattcatga atggtatcgg aacattgttg
     1381 tttattttta tcacaaaagg aaaagcacca gcctatttag gaagtagctt cgccttcatt
     1441 gctcccgcaa atatcattat cgcaaaattt ggatatccat acgcatgtgg tggatttgtt
     1501 gcggtaggtt tttgcggatg tttattggca atgatcatca agaaatttgg aacaaaatgg
     1561 attgatatcg tactaccatc tgccgcaatg ggacccgttg ttgcattaat tggtctagaa
     1621 ttatcttcat cggctgcaaa tacagctgga attctaggag ataacattga ccctaaaaac
     1681 gtgattgttt ttgcggttac acttggcatg gccgtaattg gaagtgtatg cttcaaaaaa
     1741 ttcttaagcg tcatcccaat cttgattgcc gtagttacag gatatttaac tgccgttgta
     1801 gtaggtattg ttgactttac tccagtcctt caggcaagct ttatctcaat tccaaacttt
     1861 caagcaccaa aattcagcat ggatgccatt ctaatgatgc ttcccgtatt attagttatc
     1921 gcatcagaac acattggtca ccaaatcgtt acaggtgaag ttgtaggaag aaacctaatc
     1981 gaagatccag gactacaccg ctctttattt gctgataact tttcaacaat gatttcaggt
     2041 ttaattggat ctgttccaac tacaacttac ggagaaaaca ttggagttat ggcgattact
     2101 ggcgtataca gcgtacaagt tattgctgga gctgccatcc tatccatcat ctgttcattc
     2161 attggtccat tatctgcatt gattcaaaca atcccaaatc cagttatcgg tggaattagc
     2221 ttcttactat acggtatgat tggtacaagt ggattaagaa ttctagtcga ccaaaaagtc
     2281 gattatggaa aaagcgtaaa catgatctta acatccgttg tattcgttgt tggacttagc
     2341 ggagtaacaa ttcacttagg aaatatttcc ttatcaggaa tggtacttgc ttgtatcaca
     2401 ggaatgatca tgtccttact attctatatc tttgaaaaat tccatttatt aaatgaagaa
     2461 gtataaacta aaaattgtca tctttggatg acaatttttt tagtgctttg catcataata
     2521 aatcgatgct tttacttttt ctgtatgctc tttaccacaa agcttttcta gaatcgcaaa
     2581 cgcaaactca atgcttgctg ctgcacttac acccgtaatg atgtttccat ctgtaacggc
     2641 atattcatat tggtattcac caccaaaatc ttcatccata tctttaaaac aagtatattt
     2701 cttacctttt aagataccct catgacctaa gattgttgga gaagcacaaa tagctgccaa
     2761 tactttattt tcggcaaact catgagctaa ttttaatact tcttcattct tttctagctt
     2821 ttgataatgt ggaccaccag gtaaaactaa acaatccaca ttgctaaagt catatgtgtt
     2881 cattggatat acactcgaat aggttatgcc aaaacgaccc gtaacctgtt cttcattgtt
     2941 gacaccaatc atatccacat caagtccacc acgtctaagt aaggcaatgg gacccatagc
     3001 ttctaattct tcaaatccat tttctaataa cactgctact ttcatgttcg tcacctcttt
     3061 agatttagta tacaacagac tataataatt gtgaaagcta actatataaa attcaagtat
     3121 tttttatgaa aaagtttgaa aatacaaaaa taactagatt ttttagttag ttttaaatag
     3181 atctttgaaa at
//
LOCUS       DBMDWL010000207         3180 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_13809_length_3180_cov_3.420266, whole genome shotgun sequence.
ACCESSION   DBMDWL010000207 DBMDWL010000000
VERSION     DBMDWL010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13809_length_3180_cov_3.420266"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="ACZPDN_10755"
     CDS             <1..406
                     /locus_tag="ACZPDN_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299138.1"
                     /translation="SQFEILENETILLSNQKSDGITLSALSNTPNPSKITAADTQYNL
                     LVTHMPDTLTNEELSTKSISLALCGHSHGAQITFPILGGYKTIDGAKSLNHSRAKKIS
                     FPYIISSGVGCTHVNLRFMSTPEIYYFMLQSK"
     gene            complement(432..1667)
                     /gene="codA"
                     /locus_tag="ACZPDN_10760"
     CDS             complement(432..1667)
                     /gene="codA"
                     /locus_tag="ACZPDN_10760"
                     /inference="COORDINATES: protein motif:HMM:NF006685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HIR1299139.1"
                     /translation="MLIKNACIENNEELKCIRIEDGKFKEIKKDLEPYAQEEMMDLEG
                     KLVLPPFVESHVHLDTCLTAGQPHWNMSGTLFEGIERWEERKEFLTKKDVMDRVNKVI
                     RMQASHGVQHVRTHVDINDPNLTALEAILELREEVKDFMDIQIVAFPQYGILSYPKGK
                     ELLETALKLGADAAGAIPHFEFTREYSVESLKICFELAQKYGKLIDIHCDEIDDEASR
                     GLETVATLAYETGMKDQVTASHTTAMHSYNNAYMIRLMRILKMSNINFVANPCVNVHL
                     GGRADTYPKRRGLTRVKELTEAGCNVSFGTDDIFDPWNPMGSGNMRDPVYMGIYTGHM
                     MGYEEILNSYKFVTTNAAKTLHLGDSYGIQEGNPASFVVMDAKNYHEALNLDAPVLKS
                     YKDGKLIASCTPATRKILF"
     gene            complement(1669..2886)
                     /gene="codB"
                     /locus_tag="ACZPDN_10765"
     CDS             complement(1669..2886)
                     /gene="codB"
                     /locus_tag="ACZPDN_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HIR1299140.1"
                     /translation="MEEIKDKDYSLEAVPESARKGFRSMFFVMLGFTFFSASMSVGAK
                     LGNGLDFKEFVLACVIGGIILSIYCGILAYIGSDTGLTMDLLCRKAFGKKGSYLSSLV
                     LGFTQIGWFGVGVAMFSIPTAQLLGINEWVLTIAAGLLMTLTAATGMKALEVVSTISV
                     PLIVVLGVYSMVTATTDGGGLVAVFAQSAGKISVFTGIGYVIGSFISGGTATPNFIRF
                     AKNNKIAVWTTVIAFFFGNTLMFCFGAIGGAFTGADDIFYVMIAQGLAIPAIIVLGAN
                     IWTTNNNALYTGGLAISNITNIQMKITTWIAGIIGTILSIWLYYNFTGWLNILNCALP
                     PIGIIVILDFFMKRKKYKNNMDVGDFNSFNIVGVIVGAIVANVLAWGIAAVNAMIVAA
                     ICYFIGYVRKKEA"
     gene            complement(2957..>3180)
                     /locus_tag="ACZPDN_10770"
     CDS             complement(2957..>3180)
                     /locus_tag="ACZPDN_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MnmM family RNA methyltransferase"
                     /protein_id="HIR1299141.1"
                     /translation="AQYALCMECWIQALNLLKVKGRMALVMYPHDTGKEEAICVEEFL
                     KTQTTIQVQKVQNLLVDHCPYLLLIEKRR"
BASE COUNT         1064 a          704 c          401 g         1011 t
ORIGIN      
        1 atctcaattt gaaattctag aaaacgaaac cattcttctg tctaaccaaa agagtgatgg
       61 cattacatta agtgcattat caaacacacc aaatcctagt aaaatcactg ctgcagatac
      121 gcaatataac ttattagtta ctcatatgcc agatacattg acaaatgaag aattatctac
      181 aaaatccatt tctttagcac tttgtggtca ttcccatgga gcacaaatca cattccctat
      241 tttaggagga tataaaacaa tagatggcgc aaaatcatta aatcattctc gcgccaaaaa
      301 gatttccttt ccatatatca tttcatctgg tgttggctgc acccatgtaa atcttcgttt
      361 tatgtcgact cctgaaatct attatttcat gctacaaagt aaataaacac aaaaaggtcc
      421 ttatggacct tttaaaatag aatttttcgt gttgccggtg tacaagatgc gatcaatttt
      481 ccatctttgt aagattttaa tactggcgca tccaaattta aagcttcatg gtaattttta
      541 gcatccatca cgacaaaact tgccggattt ccttcttgaa ttccatagct atcccctaaa
      601 tgcaaagttt tagctgcatt tgtcgttaca aatttatatg aatttaaaat ttcttcatat
      661 cccatcatat gtccagtata gattcccata tacacaggat cacgcatatt tccacttccc
      721 attggattcc aaggatcaaa aatatcatct gttccaaaag aaacattaca tccagcttct
      781 gttaattcct taactcgtgt taatccacga cgcttaggat atgtatctgc acgacctcct
      841 aaatgaacat ttacacatgg attcgccacg aaatttatgt ttgacatttt taaaatacgc
      901 attaaacgaa tcatataagc attattatac gaatgcatag ccgttgtatg gctggccgtt
      961 acctgatctt tcatgccagt ttcataagca agtgtagcca ctgtttcaag tccacgacta
     1021 gcttcatcat caatttcatc gcaatgaata tcaattagct ttccatattt ttgagccaat
     1081 tcaaaacaaa tttttaaaga ttcgaccgaa tattcacgcg taaattcaaa gtgaggaata
     1141 gctccagctg catctgctcc caatttcaaa gctgtttcca acaattcttt tccctttgga
     1201 tagctcaaaa ttccatattg aggaaaagca acaatttgaa tgtccatgaa atcctttact
     1261 tcttctctta attccaagat agcttctaaa gccgttaaat ttggatcatt gatatctaca
     1321 tgcgtacgaa catgttgaac accatgactt gcctgcatac gaatgacttt attcacacga
     1381 tccatcacat cttttttagt taaaaattcc ttacgctctt cccaacgttc aataccttca
     1441 aataaagttc ccgacatatt ccaatgtggt tgtccagctg tcaaacaagt atctaaatga
     1501 acatgagatt ccacaaaagg tggaagaaca agtttccctt caagatccat catttcttct
     1561 tgtgcatatg gctctaaatc ctttttgatt tctttaaatt tcccatcttc aattcggata
     1621 cactttaatt cttcattatt ttcaatacac gcatttttta ttaacatttt atgcctcttt
     1681 ctttcttaca tatccaataa aataacatat ggctgcaact atcatcgcat ttaccgctgc
     1741 aattccccaa gcaaggacat tcgcaacaat agccccaaca ataacaccaa caatattgaa
     1801 actattaaaa tcacctacat ccatattatt tttatatttt tttcttttca taaaaaaatc
     1861 caaaatgaca ataattccga ttggtggcaa cgcacaattc agtatgttaa gccaacctgt
     1921 aaaattgtaa tacaaccaaa tagataagat agtaccgata attcctgcaa tccatgttgt
     1981 tattttcatt tggatatttg tgatattact aatcgcaaga ccacctgtat aaagagcgtt
     2041 attatttgtt gtccaaatat tagctcctaa aacaataata gcaggaatgg ctaaaccttg
     2101 tgcaatcatg acatagaaaa tgtcatcagc acctgtaaat gcacctccaa ttgcaccaaa
     2161 acaaaacatt aatgtatttc caaagaaaaa cgcaatgaca gttgtccaaa ccgcaatttt
     2221 attattcttc gcaaaacgaa taaaattcgg tgttgccgtt cctccagaaa taaaggaacc
     2281 aattacatat ccaatacctg taaacacact gatttttcca gccgattgtg caaacacagc
     2341 cacaagacca ccaccatcag ttgtggcagt aaccatcgaa tagacaccca atacaacaat
     2401 taaaggaacg gatatcgtac ttacgacttc taatgccttc atacctgtag cagctgtcaa
     2461 agtcatcaat aatccagctg caattgtcaa tacccattca ttgatgccta aaagttgtgc
     2521 tgttggaatt gaaaacatcg ctacaccaac gccaaaccat ccaatttgtg taaaccctag
     2581 aactaagctt gataaataag aacctttctt accaaaagct tttcgacaaa gcaaatccat
     2641 tgttagtcct gtgtcagatc ctatataggc taatattcca caataaatag ataaaataat
     2701 acctcctatg acacacgcta aaacaaattc tttaaaatcc aaaccatttc ctagttttgc
     2761 tcctacactc atacttgcag aaaagaaagt aaatccaagc atgacaaaaa acatacttcg
     2821 aaaccctttt cttgcacttt ctggaaccgc ttctaatgaa taatctttat cttttatttc
     2881 ttccatatat actccttcat ttcaaaattc actatatgat tttaacagaa aaaggtacct
     2941 atatacagta cctaaatcat cttcttttct caattaataa tagatatgga caatgatcca
     3001 ccaataaatt ctgcaccttc tgtacctgaa tcgttgtttg tgtttttaaa aactcttcta
     3061 cacaaatcgc ctcttcttta cccgtatcat gtggatacat cacaagtgcc attcttcctt
     3121 ttacttttaa aaggtttaaa gcttgtatcc aacattccat acacaaagca tattgagcca
//
LOCUS       DBMDWL010000208         3129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14162_length_3129_cov_3.260568, whole genome shotgun sequence.
ACCESSION   DBMDWL010000208 DBMDWL010000000
VERSION     DBMDWL010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14162_length_3129_cov_3.260568"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="ACZPDN_10775"
     CDS             complement(<1..286)
                     /locus_tag="ACZPDN_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR1299142.1"
                     /translation="MVKIVWDWNGTLLDDVDLCFSCINRLLVNHKLQPLSTLNQYREV
                     FTFPIEDYYKRVGFNFDKIPFSILAHEYMEDYQEKSYDCNLHADVLNVVEK"
     gene            424..1941
                     /locus_tag="ACZPDN_10780"
     CDS             424..1941
                     /locus_tag="ACZPDN_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1299143.1"
                     /translation="MRGIYNSVTDLRRQVFTAIASMAYDDNTDYSKRMEEIPYEILPG
                     TKAKYRDSIFLERAIIGERLRLGMGLPVRDITEYTNISDGIEESTIAKKYYDDPLINI
                     IKFACNACPEKKVFVTNACQGCLSHQCTEVCPKDAIHIVNGKSCIDQEKCIKCGRCMD
                     ACPYHAITKLERPCAASCGMDAIKSDADGKAEIDYDKCVSCGMCLVNCPFGAIVDKGQ
                     IFQTIYAMKEGYEVIAAVAPAFVGQFGPAVTPDKVKAALKAVGFADVVEVAIGADLCT
                     IEEAEDFLEKVPEKQPFMATSCCPAWSVMAKKNFPDFAPYISMALTPMVLTGRLIKKE
                     KPNAKVVFIGPCAAKKLEASRKSIRSDIDFVLTFEEVMGMFNAKGIELDQITTSDPLT
                     EGTNAGRGFAVSGGVAKAVKDLILKEHPGTEVKVQAAEGLKNCKTMLMMAKAGRLNGY
                     LLEGMACPGGCVAGAGTLQPINKSSALVKKYATENDKKDADESAYGDRLHELSEH"
     gene            2007..>3129
                     /locus_tag="ACZPDN_10785"
     CDS             2007..>3129
                     /locus_tag="ACZPDN_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR1299144.1"
                     /translation="MHRYSYYKTAACTLNVSIGDPQANKEEILKCIQELDSDTQLVVF
                     PELCITGYTCQDLFYEHTLLNKAKQVLFEIVEELPENLVAVLGLPLEIDNKLYNCAAI
                     CFNHDILGIQVKTYIPSYNEFYETRWFSSASELKENTTLTYKNKKVPVSNHIVFKDST
                     TSACLGIEICEDLWVTIPVSSTHALAGANILCNLSASNEIISKANYRRNLVKDQSAKC
                     YAGYIYASAGPTESSSDLVFSGHNLICENGAILNETKTDKIIYGQIDLDHLNHDRLHY
                     KTSMQDLFHVNYTTVDFASKPIHEIEFDRYIDAYPFVPNNQDERIVRCLEILHIQAQG
                     LATRLSKIHCKDVVIGISGGLDSTLALLVCHEAFKINNYD"
BASE COUNT         1095 a          511 c          569 g          954 t
ORIGIN      
        1 ccttttctac aacattcaat acatctgcat gtaaattaca atcataactt ttttcttgat
       61 aatcttccat atattcgtga gccaatatgg aaaaaggtat tttatcaaaa ttaaagccta
      121 cccttttata ataatcctct attggaaatg taaatacttc tcgatattga tttaaagtgc
      181 ttaacggctg tagtttatga ttcactaaaa gtcggttgat acatgaaaaa cataaatcga
      241 catcgtctaa caaagttccg ttccaatccc atacaatttt taccaaacaa ttcaccccgc
      301 acggtattat atcactatta gaaaaaaata ataatatttt atactaatta aaactttcac
      361 aaatagactt tttactatat aatattgtat gagtctatat gaggaataaa aataggagga
      421 aatatgagag gaatttataa ctcggtaact gaccttcgtc gtcaggtctt tactgccatc
      481 gcttcaatgg cgtacgatga caatacagat tattcgaaac gtatggaaga gattccatat
      541 gaaattttac ctggtacaaa agcaaaatat cgtgacagca tctttctaga acgtgcaatc
      601 attggcgaaa gattacgttt aggtatgggc ttacctgttc gtgacattac agaatataca
      661 aacatctctg atggaattga agaaagtacg atcgctaaaa aatactatga tgatccactt
      721 atcaacatta ttaaatttgc atgtaatgca tgtccagaaa agaaagtatt tgtcacaaac
      781 gcttgtcaag gatgtttatc acaccaatgt acagaagttt gtccaaaaga tgcgattcat
      841 atcgtaaatg gtaaaagctg tattgatcaa gaaaaatgta ttaaatgtgg acgttgtatg
      901 gatgcatgtc cttatcacgc aatcactaaa ttagaacgtc catgtgcggc tagctgcggt
      961 atggatgcaa ttaaatccga tgcagatgga aaagcagaaa ttgattatga taaatgtgtt
     1021 agttgtggaa tgtgtctagt taactgccca tttggagcta tcgttgataa aggacagatt
     1081 ttccaaacaa tctatgccat gaaagagggt tatgaagtca ttgcagctgt tgcccctgct
     1141 ttcgttggtc aatttggtcc agctgtaact ccagataaag taaaagctgc attaaaagct
     1201 gtaggttttg cggatgttgt agaagttgcg attggtgctg acttatgtac aattgaagaa
     1261 gcggaagact tcttagagaa agttcctgaa aaacaaccat ttatggctac aagttgttgt
     1321 cctgcatggt cagttatggc gaagaaaaac ttccctgact ttgcaccata tatttcaatg
     1381 gcattaactc caatggtatt aacaggtcgt ttgatcaaaa aagaaaaacc aaacgctaaa
     1441 gttgtattta tcggtccttg tgctgccaag aaattggaag caagccgtaa atcaatccgc
     1501 agtgatatcg acttcgtatt aacttttgag gaagttatgg gtatgttcaa tgcgaaaggt
     1561 attgaattag accaaatcac aacttcagat ccattgacag aaggaacaaa tgcaggtcgt
     1621 ggatttgccg tgagtggtgg tgttgccaaa gctgttaaag atttaatcct aaaagaacat
     1681 cctggaactg aagttaaagt tcaagctgct gaaggattaa agaactgtaa aacaatgttg
     1741 atgatggcaa aagccggaag actaaacgga tacctattag aaggtatggc atgtcctggt
     1801 ggatgtgttg caggtgctgg tactttacaa ccaatcaaca aatcaagtgc attagttaaa
     1861 aaatatgcta ctgaaaacga taaaaaagat gcagatgaat ctgcatatgg agatcgtctt
     1921 cacgaactaa gtgaacatta atcaaagacg tccttaaaat ggacgtcttt ttttgatata
     1981 atttttaatg gaaatgaggt atcaacatgc atcgttattc atattataaa acggctgcct
     2041 gtactctaaa tgtttcaatt ggagatccac aggcaaacaa agaagaaatt ttaaaatgta
     2101 ttcaagaatt ggattcagat acacaacttg ttgtattccc agaactatgt attacaggat
     2161 atacatgtca agatttattc tatgaacaca cattattaaa taaagcaaaa caagttctat
     2221 ttgaaattgt cgaagaacta ccagaaaacc ttgttgcagt cttaggcttg cctttagaaa
     2281 ttgataataa gctttataac tgcgctgcca tttgttttaa ccatgatatt ctaggtattc
     2341 aagtaaaaac atatatccca agctacaacg aattctatga aacaagatgg ttctcgagtg
     2401 ctagtgaatt gaaagaaaac acaacactta cttataaaaa taaaaaagtg cctgtatcaa
     2461 atcatatcgt atttaaagat tcaacaacat cggcttgttt aggaattgaa atatgtgaag
     2521 atctatgggt gacaattcca gtaagttcaa cccatgcact agctggagca aatatattat
     2581 gtaatctaag tgcttccaat gaaatcatct caaaagcaaa ttacagaaga aatttagtca
     2641 aggatcaaag tgctaaatgt tatgcaggct atatttatgc tagtgctggt cctacagaga
     2701 gttctagtga tttagttttt tctggacaca atttaatttg tgaaaacggg gctatcttaa
     2761 atgagacaaa aacagataaa attatttatg gacaaattga tttagaccac ttaaaccatg
     2821 atcgtctaca ctataagaca agcatgcaag atttattcca tgtaaattat acaaccgtag
     2881 atttcgcatc aaaaccaatc catgaaattg aattcgatcg ttatattgat gcttatcctt
     2941 ttgttccaaa taatcaagat gaaagaattg ttcgttgctt agaaatatta cacattcaag
     3001 ctcaaggctt agcaacacgt ctttctaaga ttcactgtaa agatgttgtg attggaatta
     3061 gtggtggact tgattccact ttagctttac ttgtatgcca tgaagccttt aaaatcaaca
     3121 attatgatt
//
LOCUS       DBMDWL010000209         3122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14208_length_3122_cov_1.675935, whole genome shotgun sequence.
ACCESSION   DBMDWL010000209 DBMDWL010000000
VERSION     DBMDWL010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14208_length_3122_cov_1.675935"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="cls"
                     /locus_tag="ACZPDN_10790"
     CDS             <1..1094
                     /gene="cls"
                     /locus_tag="ACZPDN_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1299145.1"
                     /translation="GFEYYAPCENGFDEMLKELKKAEKYIFMEYFIIEEGIMFNSILS
                     ILEEKVKQGVEVRIMYDDMGSLGTLPASYAKQLEKKGIKAVSFNRISPIINTIMNHRD
                     HRKILVIDGRVAFSGGANLADEYINKKMKYGHWMDNIICVKGKAVWSYLIMFLTNWNA
                     LRHEDDDYTVFKNSKIVDETSFDGYIAPYAETPLDQELTGQSVYEDLLNSANEYVYIM
                     TPYLIIDSEMINTLIHTAKKGVDVRIIMPGIADKQIVHDMGTTYYAQLIQGGVKIYEY
                     EPGFVHSKVFVCDDTYATVGTINLDYRSLYLHFENGTLLYKSKKILNVKQNFIDTLSK
                     CKEIQIEDTHVNIVKGLFLSIIRIFSPLL"
     gene            complement(1131..2063)
                     /locus_tag="ACZPDN_10795"
     CDS             complement(1131..2063)
                     /locus_tag="ACZPDN_10795"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HIR1299146.1"
                     /translation="MKYGFGVDIGGTTIKIGLFSVEGDLIEKWEIPTNKTDNGKHILK
                     EIADFIHRTIEEKNIDKTDVLGVGLGVPGPVNKNGVVNGCVNLGWNAFNVEKEFNEIS
                     GFSVKVGNDANVAALGEMWQGAGKGYQDVLMVTLGTGVGGGCVLDGKIVSGIHGAGGE
                     IGHMPVKNDETIACNCGNHGCLEQYVSATGIVTQAKKTLEQDTRSSSLRNYASLEAKN
                     IYDEAKNGDEIANEVVNLTCKILAKSLAQVCCVIDPEIIVIGGGVSKAGDILTSRIST
                     FFKAYAFHACENTPIVLAKLENDAGMYGCVKSLF"
     gene            complement(2112..2777)
                     /locus_tag="ACZPDN_10800"
     CDS             complement(2112..2777)
                     /locus_tag="ACZPDN_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299147.1"
                     /translation="MSDCYLTLEEVKLNTSYKTYITNFFSYTKARHRIHYLYDENDNV
                     HSVELYEIIDRKVDPETNDCLIRAEFEFEDHEDMDDLLHKPLYFREPEWFCDLTNEEI
                     VQLDCIFEQYEPVLGPLIERNNISIFMDGTVQINDKIYHFSKPIIYDFFDEIRINLGS
                     EKTYKPLLSGACSSRYTLHLKSGKVIKYSGDYALCDGTPYGCKRYTKLIVDDLIKQVY
                     ESD"
     gene            complement(2759..>3122)
                     /locus_tag="ACZPDN_10805"
     CDS             complement(2759..>3122)
                     /locus_tag="ACZPDN_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299148.1"
                     /translation="DWDILNLLNHNTSNKTINISICDDGMIQIKDTKENQNPLISTYQ
                     VNKTYIQQFFKAMKLNLGSENTYEPSYVGGWSRSFTLYCESGNVFEYHGNYCTDFNEE
                     SLTTNIVEELLTKCQIVI"
BASE COUNT         1040 a          553 c          438 g         1091 t
ORIGIN      
        1 ccggttttga atattatgct ccatgtgaaa atggttttga tgagatgtta aaagagctaa
       61 aaaaagcgga gaaatatatt tttatggaat atttcattat tgaagaaggc atcatgttta
      121 actccatact ttctatatta gaagaaaaag taaaacaagg cgttgaagta cgtattatgt
      181 atgatgacat gggatcatta ggtacacttc ctgcttctta tgccaaacaa ttagagaaaa
      241 aagggattaa agcagtgtct tttaatcgaa tctcccctat tatcaataca attatgaatc
      301 atagggatca tcgaaaaata ttagttattg atggaagggt tgcctttagt ggtggtgcta
      361 atttagctga tgaatatatc aataaaaaaa tgaaatatgg tcattggatg gacaatatca
      421 tttgtgttaa aggtaaagct gtatggtctt atctaatcat gtttttgaca aactggaatg
      481 ctttacgcca tgaagatgac gattatactg tttttaaaaa ctccaagatt gttgatgaaa
      541 catcttttga tggatatatt gctccctatg cagaaacacc tttagaccaa gaactaacag
      601 gacaatctgt ttatgaagac ttgttaaata gtgccaatga atatgtatat attatgacac
      661 cctatttaat tattgattct gaaatgatca acacgttaat tcataccgca aaaaaaggtg
      721 tcgatgtacg aattattatg ccaggcattg cggataaaca aatcgtgcat gacatgggta
      781 caacttacta tgcgcaattg attcaaggtg gtgttaaaat ctatgagtat gaacctggat
      841 ttgtccactc taaagtcttt gtatgtgatg atacatatgc cacagtagga acaatcaatt
      901 tggattatcg ttcactatat ctacattttg aaaatggtac tttattatat aagtcaaaaa
      961 agattttgaa tgtgaaacaa aactttatag atacattgtc aaaatgtaaa gaaattcaaa
     1021 ttgaagatac ccatgttaat attgtcaaag gattattctt aagtattatt cgaatctttt
     1081 caccattatt atagaaaaag caaggttaaa tccttgcatt tttagtagaa ttaaaataat
     1141 gattttacac agccatacat tccagcatca ttttcaagtt ttgccaaaac gataggtgta
     1201 ttttcacatg catggaatgc atacgcttta aagaatgtgc taattctaga agttaagata
     1261 tctccagctt tagaaacgcc accaccaatc acaatgattt ctggatcaat tacacaacat
     1321 acttgtgcaa gtgatttagc taagatttta caagttagat tgacaacttc attggcgatt
     1381 tcatccccat ttttagcttc atcataaata ttcttggctt ctaaagatgc atagtttcgt
     1441 aaactcgaac tacgagtatc ttgttctaat gttttcttag cttgtgtaac tattccagta
     1501 gctgaaacat attgttctaa acatccatgg ttaccacagt tacatgcgat cgtttcatca
     1561 ttctttacag gcatatgacc aatttcccca cctgcaccat gaattccaga aacgattttt
     1621 ccatctaaaa cacaaccgcc tccaacacca gttccaagtg taaccatcaa tacgtcttgg
     1681 tatccttttc ctgccccttg ccacatttct ccaagggctg ctacattggc atcatttcct
     1741 acttttacac taaagccaga aatttcatta aattcctttt cgacattgaa tgcgttccaa
     1801 cctaagttta cacatccatt cacgacaccg tttttattta caggtcctgg cacacctaaa
     1861 cctacaccta aaacatccgt cttatcaatg tttttttcct cgatagtacg atgaataaaa
     1921 tcagcaattt ctttaagaat atgttttcca ttatcagttt tatttgtagg aatctcccat
     1981 ttttcaatca aatccccctc gactgaaaat aaaccaatct taatggtagt accaccaata
     2041 tcgactccaa atccatattt catattatat aatctccttt aataatagct tattaaaatt
     2101 ctcaataaga atcaatcact ttcatatact tgtttgatta aatcatcgac aattaatttt
     2161 gtatatcttt tacatccata tggagtgcca tcacataatg cataatcacc agaatattta
     2221 atgactttac cactttttag atgcaaagta tatcggctac tacacgcacc acttagtaat
     2281 ggtttatatg ttttttcact gcctaaattt attcgaattt catcaaagaa gtcataaata
     2341 attggcttag aaaaatgata tattttatca tttatttgca ctgttccatc cataaatata
     2401 gaaatattat ttctttcaat cagtggcccc aaaacaggtt catattgttc aaaaatacaa
     2461 tccagttgta caatttcttc atttgtcaaa tcacaaaacc attcaggttc tctaaaataa
     2521 agtggtttat gaagaagatc atccatatct tcatgatctt caaactcaaa ttcagcacga
     2581 atcagacaat catttgtttc tggatctact tttcgatcta ttatttcata caattcaaca
     2641 gaatgcacat tgtcattttc atcataaaga taatgaattc tatgtcgagc ttttgtgtat
     2701 gaaaagaaat tagtaatata cgttttatat gatgtgttga gttttacctc ttctaaagtt
     2761 aaataacaat ctgacatttt gtaagtaact cctctactat atttgtcgta agactttctt
     2821 cattaaaatc tgtacaataa ttgccgtgat attcaaacac attcccgctt tcacaatata
     2881 atgtaaaact tctagaccat ccaccaacat agcttggctc atatgtgttt tcacttccta
     2941 gatttagctt catagcttta aagaattgtt gaatataagt cttgttaact tgatatgttg
     3001 aaattaaagg attttgattt tcctttgtat ctttaatttg aatcattcca tcatcacaga
     3061 tagatatatt tatagtttta ttacttgtgt tatgattaag tagattcaat atatcccaat
     3121 cc
//
LOCUS       DBMDWL010000210         3121 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14211_length_3121_cov_3.215563, whole genome shotgun sequence.
ACCESSION   DBMDWL010000210 DBMDWL010000000
VERSION     DBMDWL010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14211_length_3121_cov_3.215563"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="ACZPDN_10810"
     CDS             <1..854
                     /locus_tag="ACZPDN_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HIR1299149.1"
                     /translation="PLTRKKDVIKICEMHPDCAFLSFTNGTLIDEEFCQDMLRVKNFV
                     PAISLEGFETANDSRRGEGCYNSVKRAMELLKKHKLPFGISTCYTSANYKDIASDEFF
                     DLMIDSGALFCWFFHYMPVGNGAVKELLPTPEQRAYVYHQIRKFRSEKPIFTMDFQND
                     AQYVGGCIAGGRRYLHINAKGDVEPCVFIHYSNCNIHDTTLLDALRSPLFQAYHDNQP
                     FNENMLRPCPMLENAGRIQELVKQSKAVNTDYESPEDVDHLLEKTVPYAKNWKPMADK
                     LWNEQKK"
     gene            905..1825
                     /locus_tag="ACZPDN_10815"
     CDS             905..1825
                     /locus_tag="ACZPDN_10815"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1299150.1"
                     /translation="MKKNNKRFSGISKLPNGNTSNKIVPGCIVLEGGAFRGLYTSGVL
                     DALMQFEINMQCTVGTSAGALNGFNYVAGQIGRSARINLGYRHDSRYVGLEAFKNNKG
                     IIGFDFLMKGTQYFDPLNTPRFMNPTRDFIAVCTNCKTGRPEYFSKNKTQDIFKAVQA
                     SATMPYVSKMVEIEDGLYLDGGCSDKIAYQWALDHDFEKIIVVRTRQREYRKSDTNPL
                     VQKMTKRLYAGYPALEQKLESMNSDYNKQCDELARLENEGRIFTIAPSKPVNVSRVER
                     DVDKLADLYYMGYLDGLNQIQRIKEYLEID"
     gene            1877..2170
                     /locus_tag="ACZPDN_10820"
     CDS             1877..2170
                     /locus_tag="ACZPDN_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299151.1"
                     /translation="MVQERQQEIVNELLNYMDTLEDGSEVSTWDLMDEVFNYQRFNDG
                     YCFNDFKISEEEFISLDSLLKESAMKEGFFIDDSIYQDVIGAFPFSLTFVVRK"
     gene            2173..3060
                     /locus_tag="ACZPDN_10825"
     CDS             2173..3060
                     /locus_tag="ACZPDN_10825"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR1299152.1"
                     /translation="MEKLETLIQELYQEGTREKNMEVLSHIRKLAKEQKQFIIPVGDA
                     EGEKVYRRLSLDDGQDVFVAFTTQAQVDLGQTTETLNQSVMDVLNMVHDTQGVSGVVL
                     NPWKDSYFLPKVLIEMILDNKNLESEIKVVKGDITTFNGDCIVNAANESLLGGGGVDG
                     AIHRVAGPMLLEECKLLNGCQTGQAKITKGYDLKVKYVIHTVGPVYSGKHEDEHMLRD
                     CYWNSLTLARKYDIHTIAFPAISCGVYGYPVEKAVPLVLKTIADWLDANSDYTMKISL
                     YCFDEETTKEYQKYTSLTL"
BASE COUNT         1066 a          405 c          678 g          972 t
ORIGIN      
        1 aacctttaac acgtaaaaag gatgtgatta aaatttgtga aatgcatccc gattgtgctt
       61 tcttatcttt cacaaatggt acattgatcg atgaagaatt ctgtcaggat atgttgcgag
      121 ttaaaaactt tgtgccagcc attagtttgg aaggttttga aacagcgaat gattctagac
      181 gtggtgaagg ttgttacaat tctgtaaaac gtgccatgga attattaaag aaacacaaat
      241 tgccttttgg aattagtact tgttatacaa gtgcaaacta taaagatatc gcaagtgatg
      301 aattctttga tttaatgatt gattcaggtg ctttattctg ttggttcttc cactatatgc
      361 cagtaggaaa tggggctgtt aaagaattac ttccaacacc agaacaaaga gcgtatgttt
      421 atcatcagat tcgtaagttc agaagtgaaa aaccaatttt cactatggat tttcaaaatg
      481 atgcgcaata tgtgggtgga tgtattgccg gaggaagaag atatcttcat atcaatgcca
      541 aaggggatgt agaaccatgt gtatttattc attattcaaa ctgtaatatt catgacacga
      601 cattacttga tgcactaaga tctccattat tccaggctta tcatgataat caaccattta
      661 atgaaaatat gttaagacct tgtccaatgc ttgaaaatgc aggtcgcatt caagaattgg
      721 taaagcaaag taaagcagtc aatacagatt atgaatcccc agaagatgtg gatcacttat
      781 tagaaaagac agttccatat gcaaaaaact ggaagccaat ggctgacaag ctttggaatg
      841 aacaaaaaaa gtagacaaat ttaaaaaatg agtataaaat tttcagctaa gaaggaggga
      901 tgttatgaag aagaataata agcgtttttc gggaatatcc aaacttccaa atggaaatac
      961 ttcaaataaa attgttcctg gatgtattgt cctggaaggc ggtgcctttc gaggattata
     1021 tacatcagga gtattagatg ctctaatgca gtttgaaatc aatatgcagt gtacagtagg
     1081 tacaagtgca ggtgcactaa acggatttaa ctatgtggct ggtcaaattg gtcgtagtgc
     1141 tagaattaat ttagggtatc gtcatgattc tcgatatgtt ggtttagaag cttttaaaaa
     1201 caacaaagga atcattggat ttgatttttt gatgaaagga actcaatatt ttgatccatt
     1261 aaatacgcct cgttttatga atcctacgcg tgattttatt gcggtatgta cgaattgtaa
     1321 aacgggaaga cccgaatatt ttagtaagaa taaaacgcaa gatattttta aagccgtaca
     1381 agctagtgca accatgcctt atgtttcaaa gatggttgaa attgaagatg gtttatattt
     1441 agatggtggc tgcagtgata agatagcgta tcaatgggcg ttggatcatg attttgaaaa
     1501 gattattgtc gttcgtacac gtcaaagaga gtatcgaaag agtgatacga atcctttggt
     1561 tcaaaaaatg acgaaaagat tgtatgcagg ctatccagct ttagaacaga agctagaatc
     1621 aatgaatagt gactacaata aacagtgtga tgaattagca agacttgaaa atgagggaag
     1681 aatctttacg attgccccaa gcaagcctgt gaatgtatcg cgtgtagaga gggatgtgga
     1741 taaactcgcc gatctttatt acatgggata tttggatggc ttgaatcaga ttcaaagaat
     1801 caaagaatat ttagaaattg actaagaggc catgcctctt tttcattttg tgttagaata
     1861 agtgtaggtg atttttatgg tacaagaaag acaacaagaa atcgtaaatg aattattaaa
     1921 ctatatggat acattagaag atggtagcga agtcagtact tgggatttaa tggatgaagt
     1981 atttaactat cagcgtttca atgatggata ttgttttaat gattttaaaa tcagcgagga
     2041 agaatttatt tcgttagatt cactattaaa ggaaagtgca atgaaagagg gattctttat
     2101 tgatgattca atctatcaag atgtgattgg agcttttcct tttagtttga catttgtagt
     2161 aaggaaatag gtatggaaaa attagaaaca ttgattcaag aattatatca agaaggaact
     2221 cgtgaaaaga atatggaagt tctttcgcat attcgtaaac tggcaaaaga acaaaaacaa
     2281 tttattattc ctgtcggtga tgcagaaggt gaaaaggtct atcgaagatt atctttagat
     2341 gatggtcaag atgtatttgt cgcttttacg acgcaggctc aagtggattt gggtcagact
     2401 acagaaacat taaatcaaag tgtgatggat gtactaaata tggtgcatga tacacaaggt
     2461 gtaagtggag ttgtattaaa tccttggaaa gattcatatt ttttaccaaa ggttttaatt
     2521 gaaatgattt tagacaataa gaatctagag agtgaaatca aagtagtaaa aggagatatt
     2581 actactttta atggagattg tattgtcaat gcagcgaatg aatcgctttt gggtggcggc
     2641 ggtgtagatg gcgctattca tagagtggct ggtcccatgt tacttgaaga gtgtaaatta
     2701 ttaaatggct gtcaaacagg gcaggccaag attacaaagg gctatgattt aaaggtaaag
     2761 tatgtaattc atactgtggg tccggtgtat tctggtaaac atgaagatga gcatatgttg
     2821 agggattgtt attggaatag cttaacgtta gctcgtaagt atgatattca tacaatcgca
     2881 tttcctgcta tttcttgtgg tgtttatggc tatccagtag aaaaggcagt gccacttgta
     2941 ttaaagacga ttgcggattg gttggatgcg aatagtgatt atacaatgaa gatcagtttg
     3001 tattgttttg atgaagaaac gacaaaggag tatcaaaaat atacatcctt aactttgtaa
     3061 aaaatgtata actacaattg aatttttaaa tgaatagttg aaatttcctg tatttatgga
     3121 c
//
LOCUS       DBMDWL010000211         3116 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14256_length_3116_cov_2.104146, whole genome shotgun sequence.
ACCESSION   DBMDWL010000211 DBMDWL010000000
VERSION     DBMDWL010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14256_length_3116_cov_2.104146"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="ACZPDN_10830"
     CDS             complement(<1..521)
                     /locus_tag="ACZPDN_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1299153.1"
                     /translation="MMKILVVEDDTLIREGLSEFLSESGYSIIQAKDGKEALEKFNTD
                     IHLVILDIQIPYINGLDVLKEIRKESDLPVLILTAFSNEEFKIDAYTNLADGYIEKPF
                     SLPVLKARIDALLQKQDIFAYKNVCVNFKSYTAKIDGKTMDINAKELEILKYLLDNAG
                     IALTRAQILDHVW"
     gene            complement(518..1882)
                     /locus_tag="ACZPDN_10835"
     CDS             complement(518..1882)
                     /locus_tag="ACZPDN_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1299154.1"
                     /translation="MFKNAFKYVTRKSVKSIILFLVVVIMSSLCLVGLSIKDATNKAS
                     NTSLGSITNSFTMEINRQVNFGTPRGAGNVKGKDIEKICSSKDIDSYVKRINSVADLV
                     DHDIILTSNENEERLTKFKSTVMLTGVNESKKETKFVSGAYKLVEGKHLNSKDKHQIL
                     MHKDLAAKNHLKVGDKLLLRSNIYDADNEKGADETVEVTIKGLFDGHNQGGVAAAQEL
                     YENNLITDLDTAAKVYGNTEKTAVYQDATFFVKGNKDLDQVIKNLRKLDINWNAYVLV
                     KSTSNYPALQKSISGMYSVANKMFIGSLIFAGIVVTLLLLMWMNARRKEIAILMALGI
                     SKTKIFIQFVLELCMVTIPAYIFAYFIASKTASGLGTKVLNQVSTGVAKQIAKESASN
                     QLGGGAEVDGFNKMLTHIDATVEPKYIVYVIIFMSIVMLVALVVATTRLLNKNCKELM
                     NEEE"
     gene            complement(1884..2519)
                     /locus_tag="ACZPDN_10840"
     CDS             complement(1884..2519)
                     /locus_tag="ACZPDN_10840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1299155.1"
                     /translation="MSILEIKDVTYGYTKLEHILFEVNQNFECGKFYAIIGKSGAGKS
                     TLLSLLAGLDEPDSGEILFEGEDISKTGYTNHRKNHICLVFQNYNLIDYLTPLENVRL
                     VNSKASPSILLDLGLEQSQINRSVMKLSGGQQQRVAIARALTSSAPIILADEPTGNLD
                     SDTASEIIDILKARAKERNKCVIVVTHSKEVADAADVVYELKDKKLKKRGK"
     assembly_gap    2676..2685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2686..3076)
                     /locus_tag="ACZPDN_10845"
     CDS             complement(<2686..3076)
                     /locus_tag="ACZPDN_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease family protein"
                     /protein_id="HIR1299156.1"
                     /translation="MNSHTYTIKGIFEGKKHETYTGLSSDLSENTMFVDYNSLDTKMV
                     TKLTIDSKRLKQVQSLYPSSEYVVSKDKNAYQSSLESIQSMNHMIQILSCSIIVCGLV
                     VLSLVLVLWLRDRIHEIGILLSIGKSKV"
BASE COUNT          969 a          621 c          420 g         1096 t
ORIGIN      
        1 ttccaaacat gatctaatat ttgtgctcta gttaaggcaa tccctgcatt atctaaaaga
       61 tattttaata tctctagttc tttggcattg atatccatcg ttttaccatc tattttcgcc
      121 gtatagcttt tgaagttcac acaaacattt ttatatgcaa aaatatcctg cttctgtaaa
      181 agcgcgtcaa tacgagcttt taatacgggt agtgaaaatg gtttttcaat atagccatcg
      241 gccaagtttg tataggcatc aattttgaat tcttcattac tgaaagccgt taaaattaaa
      301 acgggtaaat cactttcttt acgaatttct tttagtacat ccaacccatt gatatatgga
      361 atttgaatat ccaatatcac caaatgaata tctgtattaa acttttccaa tgcctcttta
      421 ccatcttttg cctggataat actatatcca gactccgata gaaattcact taatccttcg
      481 cgaatcagtg tatcatcttc cactaccaat attttcatca ttcttcctca ttcattaatt
      541 ctttacagtt cttgtttaac aagcgtgtgg tagccacaac aagtgcgact aacattacaa
      601 ttgacataaa tataataaca tacactatat attttggttc aacagttgca tcaatatgcg
      661 tcaacatctt attaaagcca tccacttcag ctccaccgcc aagttgattt gaggcagatt
      721 cttttgcgat ttgtttagca acaccagtgc ttacttgatt caatactttt gtgcctaaac
      781 ctgatgcagt tttactagcg ataaagtacg caaagatgta ggcaggaatg gttaccatac
      841 atagttctag aacaaactga ataaaaatct tcgttttaga aatacctaaa gccattaaaa
      901 ttgcgatttc ttttcttcgt gcattcatcc acatgagcaa caataaagta acaacgatac
      961 ccgcaaaaat caaagatcca ataaacattt tatttgcgac actatacata ccacttattg
     1021 atttttgtag agccggataa ttggatgtac tttttaccag tacatacgca ttccagttaa
     1081 tatctaattt ccttagattt ttaataactt gatccaaatc tttatttcct ttcacaaaga
     1141 atgtggcatc ttgatataca gctgtttttt ctgtattgcc ataaactttg gcagccgtat
     1201 ccaaatccgt gattagattg ttttcatata attcttgtgc tgctgcaaca ccaccctgat
     1261 tatgaccatc aaataaaccc ttaatggtta cttctactgt ttcgtcagcc cccttttcat
     1321 tatctgcatc atagatattt gaacggagta atagtttatc tcccactttt aaatgattct
     1381 ttgccgctaa atctttatgc attaagattt gatgtttatc cttactattt aaatgtttgc
     1441 cctcaactaa tttataggca cccgatacaa attttgtctc ctttttcgat tcattcaccc
     1501 cagtaagcat cacggtcgat ttaaatttag taagacgctc ttcattttca tttgaagtta
     1561 aaataatatc atgatcgact aagtcagcta cactattgat tcttttcaca tatgaatcaa
     1621 tgtcttttga agaacaaatc ttttcaatgt cctttccctt aacattgccc gcacctctag
     1681 gcgtgccaaa attgacttga cgattaattt ccattgtaaa actattggta atcgagccta
     1741 atgatgtatt ggacgctttg tttgtcgcat ctttaataga gagtccgact aaacaaaggc
     1801 ttgacataat tacaacaaca agaaatagaa ttattgactt cacacttttt cgagttacat
     1861 atttaaatgc attcttaaac attctatttc cccctcttct ttaacttttt atcttttaat
     1921 tcatagacaa catcagcggc atccgctacc tctttactat gtgtcacaac aatcacacac
     1981 ttatttcttt ctttagcgcg agcctttaag atatcaataa tttcactagc cgtatcgcta
     2041 tctagatttc ctgttggttc atccgccaag ataataggtg cactgcttgt aagggcacgg
     2101 gcaatcgcaa ctctttgttg ctgccctccc gataatttca ttacactacg attgatttgg
     2161 gattgttcaa gtcctaaatc taaaagaatg gaaggcgaag ctttagaatt gactaagcgt
     2221 acattttcta atggtgttaa ataatcaatt aaattgtaat tttgaaacac aagacaaata
     2281 tgattcttac gatgatttgt atatcccgtt tttgaaatat cttctccttc aaataaaatt
     2341 tcaccagagt ctggctcatc aagtccagct aataaagata ataaggttga tttaccagct
     2401 cctgattttc caataatcgc atagaatttt ccgcactcaa agttttgatt tacttcaaat
     2461 aaaatatgtt ctagtttcgt gtaaccatac gttacatctt ttatttctaa tatactcatt
     2521 atttttttct cctaactcaa ttttgataat atttcccttg gcttattgat catgatcata
     2581 aggctcgcaa ttagtacaga aacgataata atactcatca gcaagccata acttaatgtg
     2641 aagcataacg cattatgttt gattacacta taagcnnnnn nnnnnccacc ttacttttac
     2701 ctatagacaa caagatacca atttcatgga tgcgatctct tagccataga actaagacta
     2761 aagataagac aacaagtcca cacacaatga tagaacaaga tagaatctga atcatgtgat
     2821 tcatactttg tatagattct aaagacgatt gataggcatt tttatcttta gacacaacat
     2881 attcacttga tggatataag gattgaactt gttttaaacg ctttgaatct atcgtcagtt
     2941 tagtgaccat cttagtatcc aaagaattat aatccacaaa catagtattc tcacttaaat
     3001 cggatgataa acctgtatag gtttcatgct tcttaccttc aaaaattcct ttgattgtat
     3061 aagtatgaga attcaaaact attgtatccc ctatttttaa atgattcttc ttagct
//
LOCUS       DBMDWL010000212         3097 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14366_length_3097_cov_2.308745, whole genome shotgun sequence.
ACCESSION   DBMDWL010000212 DBMDWL010000000
VERSION     DBMDWL010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14366_length_3097_cov_2.308745"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            132..1559
                     /locus_tag="ACZPDN_10850"
     CDS             132..1559
                     /locus_tag="ACZPDN_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1299157.1"
                     /translation="MTFEQMNLDQNIIQALHTLGYEKPLPVQEQVIPNILSQTDCIVK
                     SRTGSGKTASFAIPIIQNLEIDERSPQALVLTPTRELALQIQQDFDRIGTFKKIKTLP
                     IFGKQPFKFQKEDLKQRCHVVIGTPGRVLDHLQQGTLDPSKIKYVVLDEADEMLNMNF
                     LDEVQAILKYMPKKHVTCLFSATYPGVIQRMSKKYLYKPAKVDLTSTNKPNILEEAYH
                     VNEEHKELFLLHLLALKKPESCMIFCKTQARVDEVYACLEKNQVSVDKIHGGMMQEER
                     LSHMRRFKKGKVRILVCTDVASRGIDVFKVEMIINYDMPSPVETYTHRIGRSGRVNEQ
                     GLAISLVNEYDGVRKEKLEEYLGYNLPFKEEGEVYLSDLDILDSLKESNRVVVDKSKD
                     LRKGITKIYLNGGKNKKIRPGDIVGAICEIDGVTVDDIGVIQVQDHQSYVDILNDKGK
                     LVLKNLKTIKGKTLRIQKAKNGFGH"
     gene            complement(1572..2261)
                     /locus_tag="ACZPDN_10855"
     CDS             complement(1572..2261)
                     /locus_tag="ACZPDN_10855"
                     /inference="COORDINATES: protein motif:HMM:NF014089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HIR1299158.1"
                     /translation="MAYYSSLMNIRRKTLMCDLKVSDVTIQPGAMQFMVGDVQATTGI
                     KGAGDFLKKSFMAKASGESTIKPEYTGTGVLVLEPTFKHIILTDLVNWDGGMIIDDGL
                     FMACESHIKQSIVARSNLSSAIAGQEGFFNLSFSAPQGVVALECDVPKSELIKVELNN
                     DVIKIDGNMAIAWSKSLEFTVERSGKTLIGSAASGEGLVNVYRGTGRILLKPVGGITN
                     MSDVVFPKSQK"
     gene            complement(2445..>3097)
                     /locus_tag="ACZPDN_10860"
     CDS             complement(2445..>3097)
                     /locus_tag="ACZPDN_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HIR1299159.1"
                     /translation="YHFCKKHGERQEEYLNPNNYNNLSKNKNGIAKLIKMLDIMAKQN
                     RDHKYLVYQRNKYHNIPLWVIMNTLTFGQISKMFEFLPQNIQGTVCQDFGNVKKNEMI
                     KYLKVLTLYRNVCAHNERLFSYRTYIDIPDTLLHKKMNISKNGSKYIYGKNDLFSIVI
                     AFRYLLSKTDFIQFKKQLLHIFCKYEKQNSNLKLNVLFEYMGFPTNWQEITKFNKI"
BASE COUNT         1041 a          481 c          539 g         1036 t
ORIGIN      
        1 tattgatatg tatgttgatc gatttgtttc atcactaacg ttttatattt catgccttta
       61 ttgtagcgca taatagtttt tgtttctgtt aaataatatt aacatggtat aattctatag
      121 gtgataaaaa tatgacattt gaacaaatga atttagatca aaatattata caagctttac
      181 atacattagg ctatgaaaaa ccattgcccg tacaagaaca agtcattccc aacattcttt
      241 cgcaaacgga ttgtattgtt aaatcaagaa caggaagtgg taaaacggca tcttttgcga
      301 ttcctatcat tcaaaatcta gaaattgatg aaagaagtcc acaagcgttg gtacttactc
      361 caactcgaga actagcttta cagattcaac aagattttga tcgtattgga acctttaaaa
      421 aaatcaagac actacctata tttggtaagc agccatttaa gtttcaaaag gaagatttga
      481 aacaaagatg tcacgttgtg attggtacgc caggaagagt tttagatcat cttcaacaag
      541 gaacactcga tccatcaaaa attaaatatg tagtcttgga tgaagcggat gaaatgttga
      601 atatgaactt tttagatgaa gtgcaggcta ttttaaaata tatgcctaaa aaacatgtaa
      661 cttgtttatt tagtgcaacc tatcctggag tgattcaaag aatgagtaag aaatatttgt
      721 ataaaccagc taaggtggat ctaacgagta caaataagcc aaatattcta gaagaagcct
      781 atcacgtcaa tgaagaacat aaagaattat tccttcttca tttattggca ttaaagaaac
      841 cagaatcctg tatgatcttt tgtaagacgc aggcaagagt ggatgaagtc tatgcatgtt
      901 tagaaaagaa tcaagttagt gtcgataaaa ttcatggtgg tatgatgcaa gaagaaagat
      961 tatcacatat gcgacgcttc aaaaaaggaa aggtgcgtat tttagtttgt accgatgtcg
     1021 ccagtcgagg aatcgatgtt tttaaagtag agatgatcat taactacgat atgcctagtc
     1081 ccgtagaaac atatacacat cgtattggac gtagtggaag agttaatgaa caaggcttag
     1141 ctataagttt agtgaatgaa tatgatggag ttcgaaaaga aaaactagag gaatatctag
     1201 gctataactt gccatttaaa gaagagggtg aagtttattt aagtgattta gatattttag
     1261 attcattaaa agaatccaat cgtgtggtgg tcgataaatc aaaagactta agaaaaggta
     1321 ttactaaaat ctatttgaat ggaggaaaaa acaagaagat tcgtcccggc gatattgtag
     1381 gtgctatctg tgaaattgat ggtgttacgg tcgatgatat tggtgtgatt caagtgcagg
     1441 atcatcaatc gtatgtagat atcttgaatg ataagggaaa acttgtatta aagaatttaa
     1501 agacaattaa aggaaaaaca ttacgtattc aaaaggcaaa aaatggattt ggtcactaga
     1561 cccaatccat tttatttttg agattttgga aatacaacat ccgacatgtt cgtaatacca
     1621 ccaactggtt ttaataagat tctacctgta cctctgtaga cattgactaa accctcacca
     1681 ctagcggctg aaccaatcaa tgttttgcca cttctttcaa cagtgaattc taatgatttt
     1741 gaccaagcaa tggccatgtt tccatcaatt ttaatgacat cattatttaa ttctacttta
     1801 atcaattcgc tttttggtac atcacactct aaggcaacta cgccttgtgg cgcactaaaa
     1861 gataagttaa agaaaccttc ttgtcctgca atagcactgc ttagattgct tcttgcgaca
     1921 atactttgtt taatgtgaga ttcacaagcc atgaataagc catcatcaat gatcatacca
     1981 ccatcccaat tcactagatc tgttagaata atgtgtttaa aagttggttc taaaactaat
     2041 actcccgtac ctgtatattc tggtttgatt gttgattctc cgcttgcctt tgccatgaat
     2101 gatttcttta gaaaatcacc agctcctttg attcctgttg tagcctgcac atcgcccacc
     2161 ataaactgca tagctcctgg ctgaattgtg acatccgaaa ctttcaaatc acacatcagt
     2221 gtttttcttc gaatattcat taaactagaa taatatgcca tttgtgcata gttaggtggt
     2281 atatatattt atgtcaatta tgagaatatt gtgattcagt gtaactttgt ggatgggata
     2341 aatagtgtaa tgaacgtata aaagaagtgg cgggaatacc agtaatgata tttccaccgt
     2401 tttgcaactc tgaatatctg attcagagct actttgtctt gaacttatat tttgttgaat
     2461 tttgttatct cttgccagtt tgttgggaat cccatatact caaagagaac attcagctta
     2521 agatttgaat tttgtttttc gtacttacag aaaatatgca atagttgctt tttaaattgt
     2581 ataaaatctg ttttggaaag tagataacga aaagcaataa caatactgaa taagtcgttt
     2641 tttccatata tgtattttga accattttta gaaatgttca tttttttatg taacaatgta
     2701 tctggaatgt caatatatgt gcgataagaa aacaggcgtt cattatgtgc acatacgttt
     2761 cggtatagcg ttaatacctt caaatatttt atcatttcat tttttttaac atttccaaaa
     2821 tcctgacaaa ctgttccttg tatattctga ggtaaaaatt caaacatttt ggatatctga
     2881 ccaaaagtaa gtgtattcat aattacccat aatggaatat tatgatattt attacgttga
     2941 taaacaaggt atttatgatc cctattttgt ttcgccataa tatcaagcat cttaattaat
     3001 tttgcgattc catttttatt ttttgaaaga ttgttataat tgttagggtt gagatattct
     3061 tcctgacgct ccccatgctt tttacaaaaa tgataag
//
LOCUS       DBMDWL010000213         3091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14415_length_3091_cov_1.166890, whole genome shotgun sequence.
ACCESSION   DBMDWL010000213 DBMDWL010000000
VERSION     DBMDWL010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14415_length_3091_cov_1.166890"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            358..669
                     /locus_tag="ACZPDN_10865"
     CDS             358..669
                     /locus_tag="ACZPDN_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299160.1"
                     /translation="MLAESDKNKYEMPETNVENLAYESKMINIVIGKVHQIYTDVTSD
                     DIARYVRQILAEDEYHRMKPTEVAKEVVAIITGSPTQKKRRPSQKAPASTNICDYISN
                     T"
     gene            complement(1107..1358)
                     /locus_tag="ACZPDN_10870"
     CDS             complement(1107..1358)
                     /locus_tag="ACZPDN_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299161.1"
                     /translation="MNFLKKPGRILLMIMIVLAVVCPYRVHMYKTKIEEIQSGKLQID
                     DMPYAASDLHPYSAYLMAVPYIIIEVVLGSLYYVRTRDS"
     gene            complement(1415..2260)
                     /locus_tag="ACZPDN_10875"
     CDS             complement(1415..2260)
                     /locus_tag="ACZPDN_10875"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1299162.1"
                     /translation="MSEMKGLVLEGGGFRGMYTCGVIDVFMENGICFDEVVGVSAGAA
                     FGCNIKSKQIGRALRYNKRFCQDSRYSGLKSFIKTGDLYNKEFAYGIVPTILDPFDTK
                     TFRENPLKFTVVCTDIHTGNPVYHEIQNGDATDIEWIRASASIPIVSKPVKLDGYELL
                     DGGVSDSIPVNWMLERSDKTVIILTRDKSYRKEPMKYIHLLKKAFKEYPNLQKALENR
                     YIVYNKTLDEIEQLERESKVFVIRPSKPISCAMIEKDPNHLQEIYDIGRQDALHYLVD
                     LKKYL"
     gene            complement(2311..>3091)
                     /locus_tag="ACZPDN_10880"
     CDS             complement(2311..>3091)
                     /locus_tag="ACZPDN_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299163.1"
                     /translation="NETDNSTYYYYLASIKNKGKKPFNTQELYYTVTDNNEKDISVID
                     KYQSTPTYAISKGQSTYIYGYIGFPNNDQKNLGLSFNKKKQFLPFKAFDIRKASDKNV
                     KDSKDKKYVFFEDDALKISVDGSKAKYVYENNETVLKNVKIRYENKTESRITVPYITP
                     SATLEGIDLSGKGEFSSMEDIQKKDFSTNGMAPKTQSFKAKVTGYMLYFLDSKQTINA
                     EIEFHFENAAPDFTDKSTKPFVVNMNSKAFGKSNTFKIGLE"
BASE COUNT         1055 a          527 c          435 g         1074 t
ORIGIN      
        1 gaatagatta aaaatataag tatgaaatta agcaaattca ttatttactt atgatatagt
       61 aagcttggta aataaatata ttataaattt aatgagtttt atgaatttac aaaaaaatag
      121 agaagggaat ttaatggcaa gttttaaaat gagttgcttt gatctctctc atcgtataaa
      181 tctattttat tttgacgaaa ggagtgaatg tcgtaacaaa ttcttatcga ctaacttaat
      241 gaagaatata gaaaatatgg ctcaaatagt tgagacaatt aaacttgctg atgaagaaac
      301 tgaatggaac aaattggaat acttcgatag tttatataat aaaagataca gattctgatg
      361 cttgccgaaa gtgacaagaa caaatatgag atgccagaaa caaacgttga gaatttggct
      421 tacgaaagca agatgatcaa tatcgtaatt ggtaaagttc atcaaatcta cacagatgta
      481 acatcagacg atatcgcacg atatgttcga caaatcttgg cagaagatga atatcatcgt
      541 atgaaaccaa ctgaagttgc taaagaggta gttgccatca tcacaggaag tccaactcag
      601 aaaaagagaa gaccttcaca gaaagcacct gcatctacga acatctgtga ttacatctcg
      661 aatacctaag aacaaagaga ctaaatgttt ctttgttttt tttgtgcttt attgagcacg
      721 aataagtaaa cgtaattctt agtttcatga agatacagca aatctttttc gtaaacgtaa
      781 cgcttagttt catgaaaaca caaaatgtct tcaatgtata taatgtaaca tatataacac
      841 ttcgcataat ctatcttatg taatatttat gaaattaaaa atagagcaga aattcgatag
      901 aaatcgaata aatctgctta atattgatat ttggcggatt caatacatgg attgatgctt
      961 cgtattaggt attctttaat tacaaattcg tgtaagtcaa gattcttagt ttatgattaa
     1021 gtacaatatc tagatttata ttctctaatt tgatttgaga cgtatagttg ttcatctttt
     1081 ttgatttcaa gacaaatcac agagtcctat gaatctctag ttcttacata atataaggaa
     1141 cctaatacaa cttcaataat gatatatggt acagccatta aatatgctga atatggatga
     1201 agatccgatg ctgcatacgg catatcatca atttgaagct tgccagattg aatctcttca
     1261 atttttgttt tatacatatg aacacgatac ggacaaacca cagctaatac aatcataatc
     1321 attaatagaa tgcgtcctgg tttttttaaa aagttcattg tgatcactcc tgaatatttt
     1381 tataaatata tcacaattca atatgtgaaa cacttcacaa atattttttt aagtccacca
     1441 aataatgtag cgcatcctgt cttccaatat cataaatttc ttgtagatga tttggatctt
     1501 tttcaatcat tgcacacgaa attggtttag aaggtcgaat tacaaacact ttgctttccc
     1561 tttctaattg ttcaatttca tccaacgttt tattatatac gatatatcga ttttccaatg
     1621 ctttttgtag atttggatat tctttgaaag ccttctttaa aagatggata tatttcattg
     1681 gctctttacg ataagattta tctcgcgtta atatgattac agttttatcg gatctttcta
     1741 acatccaatt tacaggaatt gaatcactca caccaccatc taacaattcg taaccatcaa
     1801 gttttactgg ttttgaaaca attggaatcg atgcagaagc acgaatccat tcaatatctg
     1861 tggcatcccc attctgaatt tcgtgataaa cgggatttcc agtatgaata tccgtacata
     1921 ccactgtaaa ttttaatggg ttttctctaa atgtttttgt atcaaacgga tctaaaatcg
     1981 taggtacaat gccatacgca aactccttat tgtataaatc gcctgttttt ataaaacttt
     2041 ttaaaccaga atatcttgaa tcttgacaaa aacgtttatt ataacgaagg gctctaccaa
     2101 tctgttttga cttgatgtta cacccaaagg ctgcaccagc actaactcca actacttcat
     2161 cgaaacatat gccattttcc ataaaaacgt caatgacacc acaagtatac atacctcgaa
     2221 agcctccgcc ttcaagaact aatcctttca tttctgacat aatcatcacc tcgaaaatta
     2281 tacaacatag tgaactaaaa agaaaaggac ttattcaagt ccaatcttaa atgtattcga
     2341 ttttccaaaa gccttcgaat tcatattgac cacaaaaggc tttgtacttt tgtccgtaaa
     2401 atcaggagct gcattttcaa aatgaaattc aatttctgcg ttgatagtct gcttactatc
     2461 taaaaaatac aacatataac ccgttacttt agccttaaaa ctttgtgttt taggtgccat
     2521 tccattcgtt gagaaatcct ttttctgaat atcttccata cttgaaaatt cacctttgcc
     2581 acttaaatca ataccctcta aagtagcact tggcgtaata taaggaactg taatacggct
     2641 ttctgttttg ttttcatagc gaattttaac attttttaat accgtttcat tattctcata
     2701 tacatatttt gcttttgaac catctacact gattttcaat gcatcatcct caaagaacac
     2761 atatttctta tctttcgaat cctttacatt tttatcactc gccttacgaa tgtcaaatgc
     2821 tttaaaaggc aagaattgct ttttcttatt gaaagataaa cctaagtttt tctggtcatt
     2881 atttggaaaa ccaatatatc cataaatata cgtactttgt cctttgctga tcgcatatgt
     2941 tggtgtagat tgatatttat caatgacact aatatctttt tcgttattat ccgtaactgt
     3001 ataataaagc tcttgtgtat taaatggttt cttacccttg ttttttatac ttgccaaata
     3061 ataataatat gttgaattat ccgtttcatt t
//
LOCUS       DBMDWL010000214         3081 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14484_length_3081_cov_1.570805, whole genome shotgun sequence.
ACCESSION   DBMDWL010000214 DBMDWL010000000
VERSION     DBMDWL010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14484_length_3081_cov_1.570805"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="ACZPDN_10885"
     CDS             <1..1066
                     /locus_tag="ACZPDN_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR1299164.1"
                     /translation="PTELKRAFTEGISFDASAIDGFLDEVHSDLFLFPDPNTMSILPW
                     RSMDGSVIRMYCDIQYPDGTPFERDVRYILRKAVKKAKEKGITINFGSEFEFYLFKQD
                     ENGNNTFVPLDQGGYMDIAPLDKGENIRREICLTLSEMGIDPEVSHHEMGPGQNEIDF
                     RYSQALQAADNAATFKWVVEALANMQGLVADFSPKPIEDAPGNGMHINMSVQSHEELL
                     MPFMAGILHHIEEMTLFLNPSKDSYKRMGKDKAPSFISWSYQNRNQLIRIPATHSSNR
                     MELRSPDCSCNIYLAYALLIYAGLEGIEKNMIPDPCTDVVSVDTKRLPQSLKEAKLIA
                     SNASWLKEILGNDIVDIYVG"
     gene            1073..1621
                     /locus_tag="ACZPDN_10890"
     CDS             1073..1621
                     /locus_tag="ACZPDN_10890"
                     /inference="COORDINATES: protein motif:HMM:NF015799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HIR1299165.1"
                     /translation="MEASKVLVIGNENFLALLKNTANYQYVLSKTYNDAFSKLRNTQF
                     DIVVILYSQTQSGLLNFAKSLNAQVLMVCKQEIYDQVLYQCKDSGIILLSYPIKKQVL
                     YETFQMMKSLKSKIAMYEKKIHKLEQKYLELKMVDHCKLVLISNYHWSEEKAHHYIEK
                     IAMEQQKTKFLVAKELLEEFSK"
     gene            1618..2103
                     /gene="ybaK"
                     /locus_tag="ACZPDN_10895"
     CDS             1618..2103
                     /gene="ybaK"
                     /locus_tag="ACZPDN_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR1299166.1"
                     /translation="MKKIQKTNAMRFLDQHKVEYKAHSYEHKDGDPVDGVHVANILNQ
                     PVEKVYKTLVTASNTKEYVVFMIPVEKELDLKKCAKVAGVKSCEMIHVKDINKITGYI
                     RGGCSPLGMKKQFRTFMYEDSLNQDTIYFSAGKIGMQIEMDPKVLVDLIHVQAVDIIK
                     E"
     gene            2110..>3081
                     /locus_tag="ACZPDN_10900"
     CDS             2110..>3081
                     /locus_tag="ACZPDN_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR1299167.1"
                     /translation="MWANESVFYQIYPFGACNAPFENDHVLEHRIHKLEDFIPHLKKL
                     NIDCVMLNPIFESRTHGYDTTDFKMLDTRLGTNEDLKEVIEKFHNNNIKIILDAVFNH
                     VGRDFFAFKDVLEKKWDSPYKDWFYIDFNGNNHYDDGFWYQDWEGNDILVKLNLQNQE
                     VVNYILSVVDFWQEEFKIDGLRLDVAYCLDKNFLQTLHNHVSRDFFLLGETLHGDYNQ
                     WMNETMLDSVTNYECYKGLYSSFNSANFYEILYSLNRQFGKDPWCLYTGKHLFNFVDN
                     HDVVRVASILEDKKQLPLIYTMLFTMPGIPCIYYGSEWGIEGKKNWND"
BASE COUNT         1030 a          383 c          668 g         1000 t
ORIGIN      
        1 accaaccgaa ttaaaaagag catttacgga aggtattagt tttgatgcca gtgccattga
       61 tggattttta gatgaagtgc atagtgattt attcttattt ccagatccaa atacgatgag
      121 tattcttcct tggcgaagta tggatggcag tgtaattcgt atgtattgtg atattcaata
      181 tccggatgga acgccttttg aaagggatgt aaggtatata ctaagaaagg ccgttaaaaa
      241 ggcaaaggaa aagggaatca caattaattt tggttcggaa tttgaatttt atttatttaa
      301 acaagatgaa aatggcaaca atacctttgt tcctttagat caaggtggat atatggatat
      361 tgccccttta gataagggtg aaaatattcg tcgtgaaatt tgtttaacct tatcggagat
      421 gggtattgat ccggaggttt cacatcatga gatgggtccg ggtcaaaatg aaattgattt
      481 tcgttattca caagctttac aggcagctga taatgccgct acttttaagt gggttgtaga
      541 ggctttagcg aatatgcaag gtttagtggc ggacttttca ccaaaaccaa tagaggatgc
      601 tccaggtaat ggtatgcata ttaatatgag tgtgcaaagt catgaagagt tgttgatgcc
      661 ttttatggct ggaatcttac atcatattga ggagatgact ttatttttaa atccatcaaa
      721 agattcgtat aagcgtatgg gtaaggataa ggcgcctagt ttcatttctt ggtcctacca
      781 aaatcgcaat caattgattc gtattccggc tacgcatagt tcgaatcgta tggagttaag
      841 aagtccggat tgtagttgta acatatatct tgcgtatgcc ttattgattt atgccggttt
      901 agagggtatt gaaaagaata tgattccaga tccttgtacg gatgtagtta gtgttgatac
      961 aaaaagactt cctcaaagtt taaaagaggc aaaattaata gcttcaaatg catcttggtt
     1021 aaaagaaata ttagggaacg atatagtgga tatttatgtt gggtaagtgc gtatggaggc
     1081 aagtaaagta ttagtaatag gaaatgagaa tttcttagct ttattaaaaa atacggccaa
     1141 ttatcaatat gtactttcaa aaacatataa tgatgccttt agcaagttaa ggaatactca
     1201 atttgatatt gtggtgatct tatattcgca aacgcaaagt ggtcttttga attttgcgaa
     1261 gagtttaaat gcgcaagtcc ttatggtgtg taagcaggaa atctatgatc aagttttata
     1321 tcaatgtaaa gatagtggta taatactttt gtcgtatccc attaagaaac aagttttgta
     1381 tgaaactttc caaatgatga agagtttaaa atcgaagatt gcgatgtatg aaaagaaaat
     1441 ccataagctt gagcagaaat atttagagct taagatggtg gatcattgta agttggtttt
     1501 gatctcaaat tatcattgga gtgaagaaaa ggcgcatcac tatattgaaa agatagcgat
     1561 ggagcagcaa aaaacgaagt ttcttgtggc caaagaatta ttagaggagt tttcaaaatg
     1621 aaaaagattc aaaagacaaa tgcgatgcgt tttttagatc agcataaagt ggaatacaaa
     1681 gcgcattcgt atgaacataa agatggtgat cctgtagatg gtgtgcatgt tgcgaatatt
     1741 ttaaatcagc ctgtcgaaaa ggtatataag acattagtaa ctgcatcgaa tacaaaagaa
     1801 tatgttgtat ttatgattcc agtagaaaag gaattggatc taaagaagtg cgcaaaagta
     1861 gcaggggtta aatcttgtga aatgattcat gtgaaagata ttaataagat cacgggttat
     1921 attcgtggtg gttgttctcc tttgggtatg aaaaagcagt ttcgtacatt tatgtatgag
     1981 gacagtttaa atcaagatac aatctatttt agtgccggta agattggcat gcagattgag
     2041 atggatccta aagtattagt ggatttaatt catgtgcaag ctgtagatat cataaaggag
     2101 taacatagca tgtgggctaa tgagagtgta ttttatcaaa tctatccttt tggagcttgt
     2161 aatgcgccat ttgagaatga tcatgttcta gaacatagaa ttcataaatt agaggatttt
     2221 attccgcatt taaagaaatt aaacatagat tgtgtcatgt tgaatccaat ttttgaaagt
     2281 cgtacgcatg gctatgatac aacggatttt aaaatgttag atacgcgtct tggtacaaat
     2341 gaagacttaa aagaagtcat agaaaaattt cacaacaaca acattaaaat tatattagat
     2401 gccgtattta atcatgtggg tcgtgatttc tttgcgttta aggatgtctt agaaaagaag
     2461 tgggattcac cttataagga ttggttctat attgatttta atggaaacaa tcactacgat
     2521 gatggctttt ggtaccaaga ttgggaaggt aatgatatct tggtgaagtt aaatcttcaa
     2581 aatcaagagg tcgtaaatta tattctaagc gtagtggatt tttggcaaga agaattcaag
     2641 attgatggtt taagattaga tgttgcatat tgtttggata aaaacttcct acaaacctta
     2701 cataatcatg taagtagaga tttcttcctt ttaggcgaaa cactacatgg agactataat
     2761 caatggatga atgagactat gttggattct gtcacaaatt atgagtgtta taagggattg
     2821 tattcaagtt ttaattcagc caacttctat gaaattttat attcattgaa tcgtcaattt
     2881 ggcaaggatc catggtgttt atatacgggt aagcatttat ttaactttgt ggataatcat
     2941 gatgtggtac gagttgcctc gattttagag gataaaaaac aattgccatt aatttatacg
     3001 atgttattta ctatgccagg tattccatgt atctattatg gtagtgaatg gggaatcgaa
     3061 gggaaaaaga actggaatga t
//
LOCUS       DBMDWL010000215         3072 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14548_length_3072_cov_1.874116, whole genome shotgun sequence.
ACCESSION   DBMDWL010000215 DBMDWL010000000
VERSION     DBMDWL010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14548_length_3072_cov_1.874116"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="ACZPDN_10905"
     CDS             complement(<1..284)
                     /locus_tag="ACZPDN_10905"
                     /inference="COORDINATES: protein motif:HMM:NF021489.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HIR1299168.1"
                     /translation="MFKLNFKKVFAFLFAFILCFSVVDIHADDGGYTIDDYKVNAIYH
                     SNNTIDVKEVIDVNFSSYRHGIYRNIPETMYINRDEKYKLKIQDINVLND"
     gene            complement(265..810)
                     /locus_tag="ACZPDN_10910"
     CDS             complement(265..810)
                     /locus_tag="ACZPDN_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR1299169.1"
                     /translation="MILIIVIIVVLVLLVLFFMSSYNKLVRLSNRVDEGFSTMDVYLK
                     KRFDLIPNLVETVKGYAKHEAGTLSSVIAQRNMAKTSKDVMDSENKITNAIHGIFAVA
                     EQYPDLKANTNFMDLQNQLKKVEEDIANSRKYYNALVREYNDVIMSVPTNIVASLFHF
                     EKKTMFEVANASERENVQVKF"
     gene            845..1699
                     /locus_tag="ACZPDN_10915"
     CDS             845..1699
                     /locus_tag="ACZPDN_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299170.1"
                     /translation="MIKLEIEKGIDIMKSFDYKTILKWILPILICLSSFCMVAQKTST
                     PKFHEQSIQYLDEKRNTVMTLAASSAAISTAITILPGDTGTPIANGLADLSSKFLLVL
                     GAIYLEKYALTLTCMVTFKYIIPLLCLAWLINNVIKWDWLRIVCIKIGIMALAMCLIV
                     PCSVKLSKTIEATYETSIQETIDNANNIQKKIKKDKENKNFFEKAAEAFSSTAQKYTK
                     MFNQSLNNFMEATAIMIVTSCVIPILVILFFLWFIQTIGGMTINTKRLTTFNPVSKVQ
                     KQFINSKE"
     gene            complement(1783..2481)
                     /locus_tag="ACZPDN_10920"
     CDS             complement(1783..2481)
                     /locus_tag="ACZPDN_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299171.1"
                     /translation="MNFIKEENLECSLLRKGLVSGLLMSTFVLTLLTTSSVIGEESKV
                     YAKSNGTDENILMRVDTKNGTEELKEKFEKQIQDIEEESSKKQEESKSVEEMIMEQPD
                     LLNDMQFIQNNYNAVTQIVYSQPSLSQYISNVNALTASGGVYYGPSGKETYYNLNMSG
                     VIDIMRGMGNNDAYWVREDGAKMLGDYIIVAADLNKYPRGTIVETSLGKGIVCDTGSF
                     TQNSDTQLDIATDW"
     gene            complement(2771..>3072)
                     /locus_tag="ACZPDN_10925"
     CDS             complement(2771..>3072)
                     /locus_tag="ACZPDN_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299172.1"
                     /translation="LKDMMNDDGKMLIRAKNQSFYMNWYPILKGETDSEFNQNVVYCK
                     NVNDMLSKLDLNVKTWIFYYVDLPSEIKEEIEAIQKVVGVQNHITVENVGYIIYK"
BASE COUNT         1067 a          584 c          398 g         1023 t
ORIGIN      
        1 tcatcattta atacgttgat atcttgaatt tttaatttat acttttcatc acgattgata
       61 tacatcgttt ctggaatatt acgataaata ccatgacgat aacttgaaaa gttaacatca
      121 atcacttctt tcacatcaat cgtattgttt gaatgataaa tcgcattgac tttatagtca
      181 tcaattgtat agcctccatc atcagcatga atatctacaa cagaaaaaca tagaatgaag
      241 gcaaacaaga aggcaaagac cttcttaaaa tttaacttga acattttctc tttccgaagc
      301 attggccact tcaaacattg tcttcttttc aaaatggaat aaagatgcca caatatttgt
      361 cggaacactc ataatcacat cattatattc acgaaccaaa gcattatagt atttacgaga
      421 atttgcgata tcctcctcga ccttcttcaa ttgattttga agatccatga aatttgtgtt
      481 ggcctttaaa tccggatatt gttcagccac cgcaaaaata ccgtgaatcg cattggtgat
      541 cttattttca ctatccataa catccttaga tgtttttgcc atatttcttt gtgcaatcac
      601 tgaactcaat gtacccgcct catgttttgc ataacccttt actgtttcta ctaagttagg
      661 aatcaaatca aaacgtttct tcaaatacac gtccattgta gaaaaacctt catctaccct
      721 atttgataaa cgaacaagtt tattgtagct agacataaag aatagtacta ataaaactaa
      781 aacaacaata ataacaataa ttagaatcat aataaaatcc tcctgacatg attatacatg
      841 atttgtgata aaattagaaa tagagaaagg aatcgatata atgaaatcat ttgactacaa
      901 aaccatttta aaatggatct tacccattct tatttgttta tcaagctttt gtatggttgc
      961 acaaaaaaca tcaacgccta agtttcatga acaatccatt caatatttgg atgaaaaaag
     1021 aaatacggtc atgacacttg ccgcatcaag tgcagccata tctacagcca ttactatttt
     1081 acctggagat acaggaacgc ctattgcgaa tggattggcc gacttaagta gtaaattctt
     1141 actcgttcta ggagctatct atttagagaa atatgctttg acactcactt gtatggttac
     1201 ctttaaatac atcattcccc tattatgttt ggcatggctt ataaacaacg tcataaaatg
     1261 ggattggtta agaatagtat gtattaagat tggaattatg gctttagcta tgtgtctaat
     1321 cgtaccttgt agtgtaaaat tgtctaagac aatagaagct acatatgaaa ctagtattca
     1381 agaaacgatt gataacgcga acaacatcca gaagaaaatc aaaaaggata aagaaaataa
     1441 aaacttcttc gaaaaagcag ctgaagcatt ttcttcaaca gcacaaaaat atacgaagat
     1501 gtttaatcaa tccttaaata atttcatgga agcaacagcc atcatgatcg taacatcttg
     1561 tgtaatacct attcttgtaa ttctattctt cttgtggttt atccaaacca ttggtggtat
     1621 gacaataaac acaaaaagac ttacaacatt caatcctgtt tctaaagtac aaaaacaatt
     1681 tataaattca aaagaataat ctatttggca tcttcggatg ccatttttaa tgaaaaacaa
     1741 aaaaagtaga agcacatgac ttctacttcg cactcatata tattaccagt ctgtcgcaat
     1801 atctaattga gtatctgaat tttgcgtaaa acttccagta tcacagacga ttccttttcc
     1861 taatgaagtt tctacaattg tacctctagg atatttgttt aaatcggctg caacaataat
     1921 ataatccccc aacattttcg caccatcttc acgaacccaa tatgcatcat tgtttcccat
     1981 accacgcata atgtcgatta ctcctgacat atttaagttg tagtatgttt ctttcccact
     2041 tggtccataa taaacaccgc cagaggcagt aagtgcattc acattactaa tgtattgtga
     2101 aagcgatggt tgcgagtaaa cgatttgtgt aacagcatta tagttatttt gaatgaactg
     2161 catatcattc aataaatctg gctgttccat aatcatttct tcaacgctct tcgactcttc
     2221 ttgtttcttg ctagattctt cctcgatatc ttgaatctgt ttttcgaatt tctcttttaa
     2281 ttcttccgtt ccattttttg tgtctacacg cattaagata ttttcatctg tgccatttga
     2341 cttagcatag accttacttt cttccccaat tactgatgat gttgttagta atgttaatac
     2401 aaatgtcgac atcaataaac ctgaaactaa cccttttctt aataaactac attctaaatt
     2461 ttcttctttt ataaagttca aaacaccctc caatcttccc cttaaacccc ataaaatacg
     2521 aggcaattgt tgtcaaataa attaaaacaa caattaatac tatacacaat tctaaaaaaa
     2581 atgcaaattt tggcctaaaa aaagggacgt tttcgaccaa aagtggcgag aacgacccat
     2641 ttgaaagcga tgttttcaaa aactaaaact aaaaaccaac tacgtttaag atggctgcaa
     2701 ttgcccaaat agctgccata atcaaattca aaatcatgta agtcagctag gataatactg
     2761 tttaaataac ttatttataa ataatatacc ccacattttc aacagtaata tgattttgaa
     2821 caccaactac cttttgaata gcttctattt cttcctttat ttcgcttggt aaatctacat
     2881 aatagaaaat ccaagtttta acatttaaat caagtttaga aagcatgtcg tttacatttt
     2941 tacaataaac aacattttga ttgaattcag aatccgtttc accttttaaa atcggatacc
     3001 aattcatata aaatgattga ttttttgccc taattaacat tttaccatca tcattcatca
     3061 tatcttttaa ta
//
LOCUS       DBMDWL010000216         3056 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14670_length_3056_cov_1.987817, whole genome shotgun sequence.
ACCESSION   DBMDWL010000216 DBMDWL010000000
VERSION     DBMDWL010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14670_length_3056_cov_1.987817"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /gene="xseB"
                     /locus_tag="ACZPDN_10930"
     CDS             complement(<1..209)
                     /gene="xseB"
                     /locus_tag="ACZPDN_10930"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1299173.1"
                     /translation="MAAKQLKFQEAMKRLDEIVALLNNNELELEEAMSLFEEGLKLSA
                     QCEKQLKKFETKIDQLMKVNQDVDE"
     gene            complement(211..1542)
                     /gene="xseA"
                     /locus_tag="ACZPDN_10935"
     CDS             complement(211..1542)
                     /gene="xseA"
                     /locus_tag="ACZPDN_10935"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1299174.1"
                     /translation="MRNEMVVSISSVIARIKNILTQTMDLDGIWIQGEISNLTKHRSG
                     HYYFSLKDKSGEMSCVMFSSYVSRLNFNVQEGMRVLVSGSINVYEQRGSLQLVIRQMK
                     QDGLGDLYLEFEKRKQDLLKAGYFDANHKKTKPEYIENIGVITGAGAAALQDVLSTIQ
                     KRWPMMKVTLYPSLVQGNFAPKSLIQSLKKADTCGHDALLIVRGGGSFEDLFCFNDVE
                     LIKTIYNLNTYIVSGVGHEVDTTLCDLVCDHRSVTPTAAAQWVTFDQYDVMNKIVKDK
                     ELLYRNMSLILNGYKSKLEQYKSHPYLKDPKAFIVDKQLKLDAYHTQMEHALSLELQK
                     MNLIKNYKEQLAFSMHQVLRDSKQNLSIIPEKLEVSMHNKMQEKRHILMRDCALLDAY
                     SPLKVLSRGYSISKVEDKVIKSIHDVKSDDIMVTRVHDGVITSRIASCKEE"
     gene            complement(1539..1916)
                     /gene="nusB"
                     /locus_tag="ACZPDN_10940"
     CDS             complement(1539..1916)
                     /gene="nusB"
                     /locus_tag="ACZPDN_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1299175.1"
                     /translation="MNRHEQRVIAMQSVYQHLLLGKDIRKCVFDVMKGSNDIDGYLYS
                     LTIGTIENKEAFEQKINELLRDDWDFDRLSMLEQAILLISFQEILANDTPKAVVINEA
                     ITLAKKYCDDNSYKLLNGVLDQL"
     gene            complement(1917..2813)
                     /locus_tag="ACZPDN_10945"
     CDS             complement(1917..2813)
                     /locus_tag="ACZPDN_10945"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HIR1299176.1"
                     /translation="MSVSFENYLVVGISSRALFNLEKENEVFEKEGLDAYREYQIVHE
                     NDVLSPGSGFGLVKNLLNINKLAKRKLVEVIVMSRNSADTSLRIIHSLEHYDMDIGRM
                     VLSGGEDISRYLSAFGVDLFLSCNENDVSNAIHAGFAAGMIYARDTKYNFPDYQIRIA
                     FDADAVLFSAESEKIYQEHGLEAFTENEILNQDVALKKGPMASLLKSLSWLQNETKDL
                     NVLRTAIVTARNKDTGVRVIKTLRAWNIVVDEVFFMQGAKKADVLACFGANIFFDDQD
                     VHALPASDVVPSARVPYREGEM"
     gene            complement(2849..>3056)
                     /locus_tag="ACZPDN_10950"
     CDS             complement(2849..>3056)
                     /locus_tag="ACZPDN_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1299177.1"
                     /translation="QGTKPFKTGIVTKVDDNKLTLSIPVKVNHKANVSDVCLNLQNKI
                     FESISYMTDFKPEAIEIQVTGFIF"
BASE COUNT          968 a          614 c          385 g         1089 t
ORIGIN      
        1 tattcatcaa catcttgatt caccttcatc aactgatcta ttttcgtttc aaattttttt
       61 aattgtttct cgcattgagc acttaacttt aaaccctctt caaataatga cattgcctct
      121 tcaagttcta attcattatt attaagtaaa gccacaattt catctaatcg tttcatggct
      181 tcttgaaatt tcaattgttt tgcggccatt ttattcctcc ttgcacgatg caatacgaga
      241 tgttattaca ccgtcatgca ctctagttac cattatatca tcacttttta catcatggat
      301 acttttaata actttatctt ccactttgga aatactatac cctcttgaaa gcacttttaa
      361 aggcgaatag gcatccaata aagcacaatc acgcatgagt atatgtcttt tctcttgcat
      421 tttattatgc atacttactt caagcttttc aggaattata gaaagatttt gttttgaatc
      481 ccttaaaact tgatgcatac tgaatgcgag ttgttcctta taatttttga ttaaattcat
      541 cttttgaagt tctaaagaaa gtgcatgttc catttgtgta tgatatgcat ccaattttaa
      601 ttgtttatct acaataaaag cttttggatc ttttaaatac ggatgactct tatactgttc
      661 taattttgat ttatagccat ttaaaatcaa agacatattt cgataaagca attctttatc
      721 ttttacaatc ttattcatca catcatattg atcgaaagta acccattgag ctgctgctgt
      781 aggcgtaaca cttctatgat cgcatactaa atcacataga gttgtatcta cttcatggcc
      841 tacaccactg acaatatacg tatttaagtt ataaattgtc tttataagtt ctacatcgtt
      901 aaaacaaaac aagtcctcaa aacttcctcc accacgaaca atcaataagg catcatgtcc
      961 acaagtatct gcctttttca aagattgaat caaactttta ggcgcaaaat taccttgtac
     1021 taaactagga tataaagtga ctttcatcat cggccaacgc ttttgaatcg tcgacaagac
     1081 atcttgtaac gcagcagcac ctgcgcctgt aattacacca atattttcta tatattcagg
     1141 cttagttttt ttgtgattcg catcaaaata accagctttt aaaagatctt gttttcgctt
     1201 ttcaaactct aaataaagat ctcctaaacc atcttgtttc atttgacgaa tgacaagctg
     1261 taaacttcct ctttgttcat atacattaat gcttcctgaa actaaaacgc gcatgccttc
     1321 ttgtacatta aaatttaatc gcgatacata gcttgaaaac ataacacaac tcatttcacc
     1381 acttttatct tttaaagaaa aataatagtg accagaccta tgttttgtca ggttgctgat
     1441 ttcaccttga atccagatac cgtctagatc catcgtttgt gttaatatat ttttaatacg
     1501 agcaataaca cttgaaatac ttacaaccat ttcatttctc atagttgatc aagaactccg
     1561 tttaataatt tgtatgagtt gtcatcgcaa tatttttttg ccaatgttat agcttcatta
     1621 atgacaacag cctttggcgt atcgttagcc aaaatttcct ggaaagaaat taataaaata
     1681 gcttgttcta acatacttaa acgatcaaaa tcccaatcgt ctcgtaataa ttcattgatt
     1741 ttttgttcaa aagcttcttt gttttcgatt gttccgattg ttaaactgta taaatatcca
     1801 tcaatgtcat ttgaaccttt cattacatca aatacacatt tacgaatatc ttttcctaat
     1861 aataagtgtt gatacacact ttgcatcgca attacacgtt gttcatgtct attcatttac
     1921 atttctcctt ctctatatgg tacacgagca cttggtacta catcacttgc aggtagcgcg
     1981 tgcacgtctt gatcatcaaa aaatatattg gcgccaaaac aagctaagac atctgctttt
     2041 ttagcacctt gcataaagaa tacttcatct acgacaatat tccatgctct taaagtctta
     2101 atcacacgaa cccccgtatc tttgtttcgg gctgtgacta tagctgttct taatacattt
     2161 aaatctttcg tttcattttg aagccaagat aaactcttta ataacgaagc cataggacct
     2221 ttttttaagg ccacatcttg atttaaaatt tcattttcag taaaagcttc taaaccatgt
     2281 tcctgataga tcttttcaga ttctgccgaa aataatacgg catctgcatc aaaggcaata
     2341 cgaatctgat aatctggaaa gttatatttt gtatctctgg cataaatcat accggccgca
     2401 aaaccagcat ggatcgcatt cgatacatca ttttcattac atgacaagaa taaatcgaca
     2461 ccaaaagccg ataaataacg gctgatgtct tctccaccac ttaaaaccat acgaccaata
     2521 tccatatcat agtgctcaag tgaatggatt atacgtaatg acgtatccgc actatttcta
     2581 gacataacga ttacttcaac taattttcgt ttggctagtt tattgatgtt taataagttt
     2641 tttactaacc caaaaccact tccaggactt aatacatcat tttcatgaac tatttgatat
     2701 tcacgatatg catccaatcc ttctttttca aaaacttcat tttctttttc caaattaaac
     2761 aaagcccttg aactaatacc taccacaaga taattttcaa aagatacact cataaatttt
     2821 aaaaaaagga aacctttgaa agatttcctt aaaaaataaa tccagtaacc tgaatttcga
     2881 tggcttcagg tttaaagtct gtcatataag aaatactttc aaagatcttg ttttgtagat
     2941 ttaaacatac atcactcaca tttgccttgt gattgacttt aacaggaata gataatgtca
     3001 atttattatc atccactttt gtgacaattc ccgtcttaaa tggttttgtt ccttgg
//
LOCUS       DBMDWL010000217         3044 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14783_length_3044_cov_1.549100, whole genome shotgun sequence.
ACCESSION   DBMDWL010000217 DBMDWL010000000
VERSION     DBMDWL010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14783_length_3044_cov_1.549100"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(132..>510)
                     /locus_tag="ACZPDN_10955"
     CDS             complement(132..>510)
                     /locus_tag="ACZPDN_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299178.1"
                     /translation="NEDQAIQLLLIAKEFQSQRLIYSQFFGDLTIMKCIVNDLYSLIQ
                     FDPNYMDLFDLYYALQKPCKVAIEILGDDHIIEAKYEDGLFYICMDDKNYEDVDQFFL
                     HAKVNEEPISTQYVNVNYIEILD"
     assembly_gap    511..520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<521..1033)
                     /locus_tag="ACZPDN_10960"
     CDS             complement(<521..1033)
                     /locus_tag="ACZPDN_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1299179.1"
                     /translation="MALTIEKAQQILDDYYDLTHTKYEDDISLIHALEFLIQETKNPE
                     YMVELGGWYYEQRQFDLAEEYYLMAASLEYVDAYECLGYIYYYGRVGQPDYKKAFHYY
                     KLASDKGNLVAAYKLADMYKNGYYVSKDYAKYVEIMKSLYPLIQGATNTFDPVPEIYS
                     RLAKIYVEEGN"
     gene            complement(1035..2213)
                     /locus_tag="ACZPDN_10965"
     CDS             complement(1035..2213)
                     /locus_tag="ACZPDN_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299180.1"
                     /translation="MRIIEFIKRLFHREKPIEIKQKKDLNSPKKMKQKKDLNPPKKMK
                     QTYVAKSTNYLDEQLDLFKTIQPGHLVFCRMPVCEDNLVDINNPHRIRPFLIIKKDKE
                     YLYGYAGSTKKKNPKIQYTLKDGHKDKKTGTFSNSYLYLNNVYRIPIQNIKGILKQVS
                     NKDLIQIQRKIQLLKNSDVYHIDLFENIEFTFEIGDIVEYKSSLYFLSKIQGDKIYGY
                     ICSNTNGNIELKGLNLFINTDMERKIKPDPHGLKYIFNMNVNRYVNKQLKKENKEVLE
                     LKEDAYISRNVSYYNYYPIGTILKDINTDEEIVYLFHYQRIPRCAKMEELYNDSYSVW
                     QFTPKYFDVEDELTINGIKDMLEGFLSKKSRMKEVNKPFIPILEMEYKKLKKQTKKVR
                     "
     gene            2334..>3044
                     /locus_tag="ACZPDN_10970"
     CDS             2334..>3044
                     /locus_tag="ACZPDN_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299181.1"
                     /translation="MVRILENANRLRKEKVFETYKRTCQNDYFDYDSVTRKEMFEHMI
                     ETYTPEYLISICTTWELKALRRLLRNQDLEDDRYRFERTALSSKFLYFDQELPEEFKK
                     NVKLAVKNIDLDQKAENDEPTIVILGIIRAFGIIEPSLIQAVCSACSFHYKSIIESAL
                     FNFWAYLKEDYQLIDDSFANEYVYWDYNEILDRIRDSRIQHERFEPKFLDQDSYISIF
                     YHGYDATNSDIKKFFIALK"
BASE COUNT          993 a          475 c          429 g         1137 t
ORIGIN      
        1 ccaacataaa atgtaagctc gaggtgaaaa cagcttaatt atatatgaga tattaaaaca
       61 attattgttt caatcttccc aaaaaaaaga aggtggtaaa actcattttg tttgagtgat
      121 taccactttt tttaatctaa tatctcaata taattcacat tgacatattg tgtggatata
      181 ggttcctcat tgactttggc atgcaaaaag aattggtcta catcctcata atttttatca
      241 tccatacaaa tatagaataa gccatcctcg tatttagctt caataatatg atcatcacca
      301 agaatttcta tggctacttt acaaggtttt tgtagggcat aatataagtc gaataaatcc
      361 atatagttag gatcaaattg aatcaatgaa tataaatcat tcacaataca tttcatgatg
      421 gttaaatctc caaagaattg agaataaatc aatctttgag attggaattc tttcgcaatc
      481 aataaaagtt gtatggcctg atcttcatta nnnnnnnnnn attaccttct tccacataga
      541 ttttcgcaag gcgagaataa atttcaggaa ccggatcaaa agtatttgta gctccttgaa
      601 tcaatggata taagcttttc atgatttcga catatttcgc ataatccttt gaaacataat
      661 aaccattctt atacatatcc gcaagcttgt aggcagccac aagatttcct ttgtcactgg
      721 caagcttata atagtgaaat gcttttttgt agtctggttg accgactcta ccataatagt
      781 agatatatcc taaacattca taggcatcta catactcaag actagcggcc attaaataat
      841 attcttccgc caaatcaaat tgtctttgtt cataatacca accaccaagt tctaccatat
      901 attcaggatt ctttgtttct tgtattaaaa actctagagc atggatcaat gaaatatcat
      961 cctcatattt tgtatgtgtt aaatcataat aatcatctag aatttgttgc gctttttcaa
     1021 ttgttaatgc catattacct aaccttcttt gtttgtttct ttaacttttt gtattccatt
     1081 tctaaaattg gaataaatgg tttattgacc tctttcatcc ttgatttctt gcttaagaaa
     1141 ccttccaaca tatcttttat accatttatt gttaattcat cctcgacatc aaaatattta
     1201 ggtgtgaatt gccaaaccga ataactatca ttatacaatt cttccatctt cgcacaacgc
     1261 ggaatcctct gataatgaaa taagtatacg atttcttcat ccgtattgat atcttttaat
     1321 atagttccga ttggataata attataatac gatacatttc tagatatgta tgcatcttct
     1381 ttcaattcca atacttcttt gttttctttt tttaactgtt tgttcacata acgattcaca
     1441 ttcatgttaa aaatatattt taaaccatgt ggatctggtt tgatttttct ttccatatct
     1501 gtattgatga atagatttaa accctttaat tcgatattac catttgtgtt tgaacaaata
     1561 tatccatata ttttatcgcc ttgaattttt gataaaaagt aaagggaaga tttatattcc
     1621 actatatctc ctatttcaaa tgtaaattca atgttttcaa ataaatcaat atgatataca
     1681 tcactatttt tcaataattg aatttttctt tggatttgaa tcaaatcttt gtttgagact
     1741 tgttttaaaa ttcccttgat attttgaatg ggaattcgat acacattgtt taaatataga
     1801 taggaattgc taaaagtgcc tgttttctta tctttatgtc catcttttaa agtatattga
     1861 atctttggat tctttttctt tgtgcttccc gcatacccat ataaatactc tttgtctttt
     1921 ttgataatca aaaagggacg tatgcgatgg gggttattga tatccactaa attgtcttcg
     1981 cacactggca tacgacaaaa gacaaggtgt ccgggttgga tcgttttaaa taaatccaat
     2041 tgttcatcta aataatttgt tgattttgcg acatacgttt gtttcatctt ttttggtgga
     2101 tttaaatctt tcttttgttt catctttttt ggcgaattca aatctttttt ttgtttgatc
     2161 tcaataggtt tttcgcgatg gaataaacgc ttaataaatt ctatgattct catgttgtgt
     2221 tcctcttaag gttaatttta tcacataagt tcaaatttca tgaattacat aatataatat
     2281 atacaacaat caaaactaga ttggacataa acaataaaaa aggaggagtt tcattggtac
     2341 gtatactaga aaacgcaaat cgtttacgca aagaaaaagt ttttgaaact tataaaagaa
     2401 catgtcaaaa tgattacttt gactatgatt ctgtgactcg taaagagatg tttgaacaca
     2461 tgattgaaac atatacacct gaatatttaa tatctatttg tacgacatgg gaattaaaag
     2521 ctttaagaag attattgcgc aaccaagatt tagaggatga tcgctatcgc tttgaaagaa
     2581 cggcactaag ctctaagttt ttatattttg atcaagagtt gccagaagaa ttcaaaaaga
     2641 atgtaaaatt ggctgtaaaa aacattgatt tagaccaaaa ggcagaaaac gatgaaccaa
     2701 ccattgtcat tcttggcatt attcgtgctt tcggtattat tgaaccctca ttgattcaag
     2761 ctgtgtgcag tgcatgttct tttcattata aatccattat tgaaagtgca ctttttaatt
     2821 tctgggctta tttaaaagaa gattatcaat taattgatga tagttttgcg aatgaatatg
     2881 tatattggga ctataatgaa attttagatc gcattcgaga tagtcgtatt caacacgaaa
     2941 gattcgaacc taaattttta gatcaagata gttatatttc tattttttat catggatatg
     3001 atgcgacaaa ttcggatatt aaaaaattct ttatagcttt aaag
//
LOCUS       DBMDWL010000218         3026 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14917_length_3026_cov_3.045812, whole genome shotgun sequence.
ACCESSION   DBMDWL010000218 DBMDWL010000000
VERSION     DBMDWL010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14917_length_3026_cov_3.045812"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="ACZPDN_10975"
     CDS             complement(<1..630)
                     /locus_tag="ACZPDN_10975"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HIR1299182.1"
                     /translation="MKLSQGQVMTTSKGYIFDIKRFATHDGKGIRTTVFFKGCPLRCK
                     WCQNPEGLSYLPQVLYMESKCMHCLSCVHASKQGGVRCVDHKICISRNIKEDWDAICD
                     VCPTLALSMDAKEYTVEKCVHEILKDEIFFKREGGVTFSGGEPFLQSDFLIDLLKVCK
                     EKGIHTAIETSLYTDLENVQKALPYLDQIYCDCKLYDENLHKQYTGVSNE"
     gene            complement(593..2899)
                     /locus_tag="ACZPDN_10980"
     CDS             complement(593..2899)
                     /locus_tag="ACZPDN_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HIR1299183.1"
                     /translation="MLDRISILKDKMLSQPRFVSIEQALIITRTYQENEDKSIPYKRA
                     LSLYNALNEIEIGMEPEELIVGNRTKGVRYGVVFPESGISWVDREFETIPTRPQDKFN
                     VHPEDIETFRKVIVPYWKGKSLEDVIKNRFGDEISAISKVVKVNQTDHAQGHICPNVK
                     EWLELGPQGYIDLARNHLSTCSKSQKEFYECVILVMQGVQKFMLRYHDLALKMNKKDV
                     AKICENLAYKPAATFHEALQSLWFLFVVLHMESNASSFSPGRLDETLYSYYKQDIASD
                     MLKESEALELIECLWLKFNEIVYLRNAHSAKYFAGFPIGFNICVGGQDQNGNDFSNEL
                     SHMFLKAQEDIGLPQPNLSVRLHEHTNDELLKHAIRVVSKGSGMPQFFNDKAIIKAME
                     DLGISHFDAMDYAIVGCVELTTQGNNLGWSDSGMFNMNKVLELTLNHGRCLLTGKQMG
                     PDYGSLSTYTSFSDLESAFEKMMDYFIDKMIPCLEEVEKAHIDILPTAFLSSVIDDCM
                     EKGMDVTRGGAHYNLSGIQMIQVANLADSLAALKKLVYDEKKIDAADLEKALKENFSN
                     NEVMRQMLLNRAPKYGNDVDWVDELGAKWALKFRNKLRKYTNYRNGEYHTGMYTVSAH
                     VPMGENVGASCDGRLAKTPLADGGLSAVYGRDLKGPTAVLKSVSHLDNSCTTNGGLLN
                     MKFLPEFFKTESGIDKFANFMRTFVDLEIPHIQFNVVRKEDLLNAKIHPDQYRSLTVR
                     VAGYTAYFTELAGELQDEIIARTSYDNI"
BASE COUNT          989 a          656 c          375 g         1006 t
ORIGIN      
        1 ttcattggat acacccgtat actgtttatg caagttttca tcatacaatt tacaatcaca
       61 ataaatctga tccaaatatg gtaaagcctt ttgtacattc tctaaatccg tatatagact
      121 tgtttcaata gccgtatgaa ttcctttttc cttacaaacc tttaataaat caatcaaaaa
      181 atcactttgt aaaaagggtt ctcccccaga aaaagtaact ccaccttctc ttttaaagaa
      241 tatttcatcc tttaaaattt catgaacaca tttttctacc gtatactctt tggcatccat
      301 actcaaagct aaagtaggac atacatcaca aatcgcatcc caatcttctt ttatatttct
      361 tgaaatacaa attttatgat ctacacacct aactccacct tgttttgatg catgcacaca
      421 actaagacaa tgcatacact tggattccat atataaaact tgtggtaaat aagacaatcc
      481 ttctggattt tgacaccatt tacaacgaag tggacacccc ttaaagaata ccgttgtacg
      541 aattcctttt ccatcgtgtg tcgcaaaacg ctttatatca aatatatatc ctttagatgt
      601 tgtcataact tgtccttgcg ataatttcat cctgtaattc tcctgctagt tctgtaaaat
      661 atgcagtata accagcaaca cgaaccgtca aagaacgata ttgatctgga tgtattttgg
      721 catttaataa atcttcctta cgaaccacat taaattgaat atgcggaatc tccaagtcaa
      781 caaatgtacg cataaaattc gcaaacttat caataccgga ttccgtttta aagaattctg
      841 gtaaaaactt catatttaat aggccaccat ttgttgtaca agaattatct aaatgcgaca
      901 ccgattttaa gactgctgta ggccccttta aatcacgacc atatacagcc gataatccac
      961 catctgccaa aggcgttttg gcaagtcttc catcacaact agcccctaca ttttctccca
     1021 taggaacatg ggcactcacg gtatacattc ctgtatgata ttctccatta cgatagtttg
     1081 tatatttcct taatttattt ctaaacttca atgcccattt ggcacccagc tcatccaccc
     1141 aatccacatc atttccatac ttaggtgctc gatttaataa catttggcgc atgacttcat
     1201 tgtttgaaaa attttctttc aaagcttttt ctaaatcagc tgcatcaatt ttcttttcat
     1261 catatacaag cttttttaaa gcagccaaac tatccgcaag attagccact tgaatcattt
     1321 gaatacccga caaattataa tgtgcaccac cacgcgtgac atccattcct ttttccatgc
     1381 aatcatcaat gacacttgat aaaaaggctg ttggcaaaat atcaatatgt gctttttcca
     1441 cttcttctaa acacggaatc attttatcaa taaaataatc catcattttt tcaaaggcag
     1501 attctaaatc agaaaacgaa gtatatgtac ttaaagaacc gtaatcaggt cccatttgtt
     1561 ttcctgtcaa caaacaacga ccatgattca aagttagttc taacacctta ttcatattaa
     1621 acatacctga atccgaccaa cctaaattat tgccttgtgt tgtaagttcc acacaaccca
     1681 caatcgcata atccatggca tcaaaatgtg atatacctaa atcttccatc gccttaataa
     1741 ttgccttatc attgaaaaat tgtggcattc cacttccctt agaaacaact cgaatcgcat
     1801 gttttaatag ttcatcattt gtatgttcat gtaagcgaac cgataagttt ggttgaggaa
     1861 ggccaatatc ttcttgtgcc tttagaaaca tatgactcaa ttcattacta aaatcattgc
     1921 cattttgatc ttgtccaccg acacaaatat tgaatccgat tggaaaaccc gcaaaatact
     1981 tagcactatg cgcatttctt aaatatacga tctcattgaa tttcaaccat aaacattcaa
     2041 tcaattctaa agcttcactt tcttttaaca tatccgaagc tatatcttgt ttataataag
     2101 aatataaagt ttcatctaaa cgacccggtg aaaaactcga tgcattgctt tccatgtgta
     2161 gcactacaaa taaaaaccat aatgactgca atgcttcatg gaatgttgca gctggtttat
     2221 aagctagatt ttcacaaatc tttgcgacat cttttttatt catttttaaa gctagatcat
     2281 gatagcgaag cataaacttc tgtacacctt gcatcactaa aataacacat tcatagaatt
     2341 ctttttgtga ttttgaacat gtgcttaaat gatttctagc taaatcaata tagccttgtg
     2401 gacccagttc taaccattcc tttacattgg gacaaatatg cccctgtgca tgatcggttt
     2461 gattaacttt tactacttta gaaatcgcac taatttcatc accaaaacga tttttaataa
     2521 catcctctaa acttttacct ttccaatacg gaacaatcac cttacgaaaa gtttcaatat
     2581 cttcaggatg cacattaaac ttatcttgag gacgagttgg aattgtttca aattcacgat
     2641 ccacccaaga aattccactt tccggaaaaa cgacaccata acgaacacct tttgttcgat
     2701 ttccaacaat caactcttct ggctccatac caatttcaat ctcatttaat gcgttataca
     2761 aagaaagtgc acgcttataa ggaattgatt tatcttcatt ttcttgatat gttcgcgtaa
     2821 taatcaaagc ttgctcaata cttacaaagc gtggttgaga caacatttta tctttaagaa
     2881 tagaaatacg atctaacata gtaagtcctc catttacaaa tccattataa aaagaactat
     2941 gcttaaaatc aatagttctt tattactttt tattactttt tattgtttgt agaatcccat
     3001 aaaattcatt catcttggca tactca
//
LOCUS       DBMDWL010000219         3015 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_14998_length_3015_cov_2.451613, whole genome shotgun sequence.
ACCESSION   DBMDWL010000219 DBMDWL010000000
VERSION     DBMDWL010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14998_length_3015_cov_2.451613"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="ACZPDN_10985"
     CDS             complement(<1..492)
                     /locus_tag="ACZPDN_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299184.1"
                     /translation="MMLIVSLGFLACIILKDPLCIFCFSFCFLLYLNERFHNKRILIV
                     FMILSLFSIVLVKPCKEAEKGIYTISEIKQSYYVAKNQRTRVLVQSDLNLNFKDQIQV
                     DSLEPIHTDDNFTLFSFTKYNASKNVFYKTKSIRILKKSNSLKSKLYTYISSQKNSKV
                     LLSL"
     gene            complement(489..965)
                     /locus_tag="ACZPDN_10990"
     CDS             complement(489..965)
                     /locus_tag="ACZPDN_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1299185.1"
                     /translation="MKRTLLFFCAIIFFVFTCINRYTPITLKETKPTLMEVEIKGAIQ
                     NPGVYTLKRNSSISKLIKMSGPLLENADTSSISFTHILQDKDVVIIPEIKEIKLISLN
                     SATSEELQTLPGIGPSIAQRIIDYRQENTFQTLEQIKDVKGIGEALFNKIKDKICL"
     gene            complement(1007..1495)
                     /gene="greA"
                     /locus_tag="ACZPDN_10995"
     CDS             complement(1007..1495)
                     /gene="greA"
                     /locus_tag="ACZPDN_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1299186.1"
                     /translation="MANEKFYVTQEGYNDLVNEHENLVHKVRQEVIVELQEARAQGDL
                     SENADYDAAREHQAQVEGRIRELEAMIKNAEIISESADAEDKKKKTVVKLGSTVKILD
                     LSDNQEVTFNIVGTIEADPFNGKLSNTTPLAEVIMDHKVGDVCTVKRVEEPYDVKILE
                     IK"
     gene            complement(1562..2650)
                     /gene="mltG"
                     /locus_tag="ACZPDN_11000"
     CDS             complement(1562..2650)
                     /gene="mltG"
                     /locus_tag="ACZPDN_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR1299187.1"
                     /translation="MPTRRKVNSVRVVLLVVVCILIIAGLAGVFVVKKDLSSTGNGDK
                     NIVFTIESGDTLDQVVENLENENLISNATVTKLYAKASHNTNFVAGTFELNDGMSVKE
                     ILSYIQDSTKLKKDALILKVPEGKWAKEIAAEISNLYDGKFSSEEILNQWNDISYIQK
                     LAKDYSFIHVDDLNNSNYKVKLEGYLFPDTYYLGKEDSIDEITRIMLDRFEVMYKENK
                     ELFSKSHYSVQQVISLASVVQFEASSKEDMEMIAGIFHNRLEQGIKLQSSVTVCYALY
                     DDFKDPKDCETNPEIDSPYNTYLHEGLPIGPILNPGDDAIKAVLAPKKTDYLFFAADI
                     YNKLDGKVHYSKTYEEHQQICEELGLNL"
     gene            complement(2640..2897)
                     /locus_tag="ACZPDN_11005"
     CDS             complement(2640..2897)
                     /locus_tag="ACZPDN_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1299188.1"
                     /translation="MLDSNCLYVKDENGNEKKMTILFTFDSDETGCQYVVFQDPDLDE
                     GEVYASRYDDKDQLIPIESEAEWDMVEEVINTFVEDEENAD"
BASE COUNT          920 a          571 c          419 g         1105 t
ORIGIN      
        1 taaagataat aatacttttg aattcttttg actcgatata taggtatata gtttggattt
       61 taatgaattg gatttcttta aaatccttat ggatttcgtt ttataaaata cattttttga
      121 cgcattatac tttgtaaaac taaagagtgt aaaattgtca tcggtatgaa tcggttctaa
      181 agaatcgact tgaatctgat ccttgaaatt taaatttaaa tcactctgta caagcaccct
      241 tgtcctttga ttttttgcga cataataact ttgtttgatt tcggatatag tataaattcc
      301 tttttcagcc tctttacaag gcttgactaa aacaatagag aataaagaga gtatcataaa
      361 tacaatcaga attcttttat tatgaaatct ctcatttaaa tacaataaga aacaaaagct
      421 aaaacaaaat atacaaagag gatcttttag aataatacag gccaaaaagc caagacttac
      481 aatcaacatc ataggcagat cttatccttg attttattaa acaaagcttc gccaatgcct
      541 tttacatctt taatttgttc taaagtttga aatgtgtttt cttgccgata gtctatgatt
      601 ctttgcgcaa tgcttggacc aatgccagga agcgtttgaa gctcttctga agtagcacta
      661 tttaaagata taagtttgat ttctttgatt tctggaatga tgacaacatc tttatcttga
      721 agaatatgcg taaaactaat acttgaagta tctgcattct ctaataaagg gccactcatt
      781 ttaataagtt ttgaaataga actattccgt ttgagtgtat agacacctgg attttgaatg
      841 gcccctttaa tttcaacttc catcaatgta ggctttgttt cttttaaagt aattggtgta
      901 tatcggttaa tgcatgtaaa gacaaagaaa atgattgcac aaaaaaagag aagagttcgt
      961 ttcataaata tcacccttct cttatacgtt attttttgat gtttttttat tttatttcta
     1021 aaatttttac atcataaggt tcttcaacac gttttacagt acaaacatcc cctactttgt
     1081 ggtccatgat cacttctgca agtggagttg tgtttgaaag cttaccattg aatggatccg
     1141 cttcaattgt accaacgata ttgaatgtta cttcttgatt gtctgataaa tctaagatct
     1201 ttacagtcga tcccaatttt acaaccgtct ttttcttttt atcttctgca tcagcagatt
     1261 cactaatgat ttcggcattt ttgatcattg cctctaattc acgaatacga ccttcgactt
     1321 gcgcctggtg ttctcttgca gcatcatagt ccgcattttc ggacaagtca ccttgtgcac
     1381 gagcttcttg taactcaacg ataacttctt gacgcacttt atgaactagg ttttcatgtt
     1441 cattgactaa atcattataa ccttcttggg taacgtaaaa tttttcattt gccatgtggt
     1501 atcactcctt gtatttagca caaaaaatta tagcataatc cttgaattat gcaaaacatt
     1561 gttacagatt taatcctaat tcttcgcaaa tctgttgatg ttcttcatat gtttttgaat
     1621 aatggacttt accatccaac ttattgtaaa tatcggccgc aaagaataga taatctgtct
     1681 ttttaggtgc aagcacagcc ttgattgcgt catcacccgg atttaagatc ggcccaattg
     1741 gcaaaccttc atgtagatac gtattgtatg gtgaatcgat ttctggattg gtttcacaat
     1801 ctttaggatc tttaaaatca tcatataatg cataacatac tgtaacactc gattgaagtt
     1861 ttatgccttg ttctaaacga ttatggaaaa tacctgcaat catttccata tcttcttttg
     1921 aacttgcctc aaattgaacc acacttgcca aactgatgac ttgttgcaca gaataatgac
     1981 tctttgagaa caattccttg ttctctttat acatcacttc aaagcggtct agcataatcc
     2041 gagtgatttc atcaatggaa tcctcttttc ctaagtaata cgtatctggg aataaatacc
     2101 cttctagttt tactttgtag ttggaattgt ttaaatcatc cacatggata aatgaataat
     2161 ctttcgctag tttttgaata tatgaaatat cattccattg atttaagatt tcttcagacg
     2221 aaaactttcc atcatataaa ttggatattt cagccgctat ttcttttgcc cattttcctt
     2281 ctggtacctt taatatcaac gcatccttct taagttttgt actatcttga atatacgata
     2341 atatttcttt tacactcata ccatcattca actcaaatgt tccagccaca aaatttgtat
     2401 tgtgacttgc cttggcatac aactttgtca ctgtcgcatt tgaaatcaaa ttttcatttt
     2461 ctaaattttc tacaacctga tcaagcgtat ctccgctttc aattgtgaat actatattct
     2521 tatccccatt gcccgtactt gacaaatctt tttttaccac aaaaacacct gcaagaccag
     2581 cgataattaa aatacataca acgactaata aaacaactct tacagaattg actttacgcc
     2641 tagtcggcat tttcctcgtc ctcaacaaat gtgttgatta cttcttcaac catatcccat
     2701 tctgcttcac tttcgattgg aatcaattga tccttgtcat cataacgaga agcataaact
     2761 tcgccctcat ctaaatctgg atcttggaaa acaacatatt gacatcccgt ttcatcacta
     2821 tcaaaagtga atagaattgt catctttttt tcgtttccat tttcatcttt aacatataaa
     2881 caatttgaat ctaacataca ttacttcctg gtatattgta attgacccat ctagctggtc
     2941 atttacaaca tacatctcct ttctttcaac ctttaataac cgtggttgaa gacggtgttg
     3001 ttaatttgta aaatt
//
LOCUS       DBMDWL010000220         3000 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_15113_length_3000_cov_1.602277, whole genome shotgun sequence.
ACCESSION   DBMDWL010000220 DBMDWL010000000
VERSION     DBMDWL010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15113_length_3000_cov_1.602277"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="ACZPDN_11010"
     CDS             complement(<1..158)
                     /locus_tag="ACZPDN_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014038.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR1299189.1"
                     /translation="MYDNIERELKLLINKQVYDNIMNSYDFHKSIVQTNTYFDTANQD
                     VKKQHGAVR"
     gene            213..986
                     /locus_tag="ACZPDN_11015"
     CDS             213..986
                     /locus_tag="ACZPDN_11015"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIR1299190.1"
                     /translation="MRFSIVDRGDENSKRVADTLKKKCFSIGWQYDDEHCEIIFSVGG
                     DGTLLRAIHTYIDKLDEIQFVAIHTGNLGFFTDYTQDEVDHLVYDLKHNKPVVEEFNL
                     LQMDLPQVNETLYALNEVRIESLAKTLVLDISIDDEFFESSQGSGICVSTQSGSTAVN
                     RALKGAVVDPGVKVLQLCEIMPISHKNHHSLKNPYIMNDNRKISVRGDTLAYAHVCYD
                     HLERDLKSISEIIIHSSTKKVRFARYRTYSYLTRLKNLY"
     gene            complement(978..2201)
                     /locus_tag="ACZPDN_11020"
     CDS             complement(978..2201)
                     /locus_tag="ACZPDN_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1299191.1"
                     /translation="MKQTIQWKNKSIQIDIGLILILILFGLTSCFALYNSFNLIKSGS
                     GYSYLLRQLFWFVVGFIAMFFLTILQNKKIYSYMDIAYKILMLCLIYLFLSRMLFNFV
                     GISLPFASEVNGAISWFQLPLIGSFQPSEFIKIVLIVLVSQIIVEHQNEHPNPTHKDD
                     LLLLWQIAKVLFPPLFLIMMQPDTGICIIIVFNIFILVCCSGIRKEYVIGIFAFLAIV
                     VGTFLFLYFEYPEILSSFISSYKLQRIDAWLEPESNIRGSSNQLYTALLSLGSAGLTG
                     YGLQANIISIPEAHTDFIFAAFGQCFGFIGTTFILILCLLLDIYLCKIAYATKDRKDR
                     FIVVGIIAMLLYQQLQNTGMIVGLLPITGITLPLISYGGSSILSYFISFAIILNISPA
                     TKRTIHLNRSRKKSI"
     gene            2289..>3000
                     /locus_tag="ACZPDN_11025"
     CDS             2289..>3000
                     /locus_tag="ACZPDN_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR1299192.1"
                     /translation="MAEKKYTPMMQHYLKMKEENPDAILFYRLGDFYEMFFDDAKLVS
                     QELDLVLTGRSAGVEEKVPMCGVPFHAANSYISRLVKKGYKVAICEQLEDPSNAKGLV
                     DRGIVKIVTPGTYMDSTMDAKTTNYMASCDISSFEITVVYCELSTGELKYQTLQRSLV
                     ALQKALLEQNVSELVCPMAMDKKWMSALEEMETILISKHKKSSVDSQDLPLLNGIESE
                     SLKEAFALLMAYLKDTQKQ"
BASE COUNT         1125 a          406 c          508 g          961 t
ORIGIN      
        1 cgaacggcac catgttgttt ttttacatct tgatttgcgg tatcgaagta tgtatttgtt
       61 tgaacgattg atttatgaaa atcatatgaa ttcataatat tatcgtaaac ttgtttgttg
      121 attagtaatt ttaattctct ttcaatatta tcgtacattt atatcaccca taaatatgtt
      181 acaatatatt aaaaaaaaga ggaagtgatt taatgcgttt tagtatagtg gatcgaggag
      241 atgaaaactc taaaagagtt gccgatactc taaagaaaaa atgtttttct ataggatggc
      301 agtatgatga tgaacactgt gagattattt tttctgtagg aggagatggc actttattaa
      361 gggccatcca tacatatatt gataaattag atgaaattca atttgttgcg atacatacag
      421 gaaaccttgg cttttttacg gactatacac aagatgaagt ggatcatcta gtttatgatt
      481 tgaaacataa taaaccagtg gtagaagaat ttaatttact tcaaatggat cttcctcaag
      541 taaatgaaac attatatgct ttaaatgaag ttcgaatcga aagtttagcc aaaacattgg
      601 tattggacat ttcaattgat gatgaattct ttgaaagtag tcaaggttca ggaatttgtg
      661 taagtactca atctggttct actgcggtta atagagctct aaaaggagcg gttgttgatc
      721 caggggtaaa ggttttacaa ctttgtgaaa tcatgcctat ctcacataag aaccatcatt
      781 ctttaaaaaa tccatatatt atgaacgata atcgaaaaat tagtgttaga ggcgatactt
      841 tagcatatgc tcatgtatgc tatgaccatt tagaaagaga tttaaaatct atatctgaaa
      901 tcatcattca ttcaagtaca aaaaaagtac gatttgcgag ataccgtact tattcttatt
      961 taacaagact aaaaaattta tattgatttt tttctagagc gattcaaatg aatcgttctt
     1021 tttgttgctg gtgaaatatt taaaataatt gcgaaagata taaaatatga aagaatacta
     1081 ctaccaccat aagatataag tggtaatgtg attcctgtaa tcggaagtaa tccaacaatc
     1141 atacctgtat tttgaagttg ttgatataga agcatggcaa taataccaac aacgataaat
     1201 cggtcttttc tatcttttgt agcgtaagca attttacaca aataaatatc tagtaataag
     1261 caaagaatta gaataaatgt tgttccaata aaaccgaaac actgaccgaa tgctgcaaag
     1321 ataaagtcgg tatgtgcttc tggaatagaa atgatatttg cctgtaaacc atagcctgtt
     1381 agaccagcac ttcctaatga caataatgcg gtatatagct ggtttgaact acctcgaata
     1441 ttgctttcag gttctagcca tgcatcaatt ctttgtagtt tatatgaaga tataaatgaa
     1501 gaaaggattt cgggatattc aaaatataaa aacaagaatg ttcctactac aatagctaaa
     1561 aaggcaaaaa ttccgatgac atattctttt cgaataccac tacaacatac taaaataaaa
     1621 atattgaaga caatgataat acatatacca gtatctggct gcatcatgat taaaaataac
     1681 ggtggaaata gcacttttgc gatttgccaa agtaataata aatcatcttt atgtgttggg
     1741 tttggatgtt cattttgatg ttcaacaata atttgtgaaa ctaaaacaat taaaacgatt
     1801 ttgataaatt cacttggctg aaaactacca atcaacggca attgaaacca ggaaatggca
     1861 ccattgactt cacttgcgaa aggaagtgaa attccaacaa agttaaataa catacgggat
     1921 aaaaacaaat aaataagaca taacattaaa attttatagg ctatatccat ataagaatat
     1981 atttttttat tttggagaat tgtaagaaag aacattgcga tgaaacctac aacaaaccaa
     2041 aataattgac gcaataaata tgaatatcca cttccggatt taattaaatt gaaagaatta
     2101 tataacgcaa aacaactagt taaaccaaaa aggattagaa ttagaatcaa accaatatca
     2161 atttgaatgg acttgttttt ccattgaatc gtttgtttca ttagtaaaaa cctccttatt
     2221 cagtgttttt aattataaca tatttatttt gaaattttgg tataatagtc atgttaggat
     2281 gatgatttat ggcagaaaaa aaatatacac caatgatgca acattattta aaaatgaaag
     2341 aagaaaatcc agatgcgatt ttattttatc gcttagggga tttttatgaa atgttctttg
     2401 atgatgcaaa attggtatca caagaattgg atttagtatt aactggaaga agtgcaggcg
     2461 tagaagaaaa agttccgatg tgtggtgttc cttttcatgc agctaattca tatatttctc
     2521 gtttagtaaa aaaaggatat aaagtagcaa tttgtgaaca attagaagac ccttctaatg
     2581 cgaaaggatt agtggatcga ggaattgtaa aaattgttac accaggaact tatatggatt
     2641 caactatgga tgccaaaact acaaactaca tggcatcgtg tgatatttct agttttgaaa
     2701 ttacagtggt ttattgtgaa ttaagtacgg gtgaattgaa atatcaaact ctacaacgat
     2761 cattagttgc tttacaaaaa gcactgttag aacaaaatgt aagtgaactt gtttgtccga
     2821 tggcaatgga caaaaaatgg atgagtgcac tagaagaaat ggaaacaata cttatttcga
     2881 aacataaaaa gtcaagtgtt gattcacaag atctaccatt attaaatgga attgaatccg
     2941 agtcattaaa agaggcgttt gcacttttaa tggcatattt aaaagataca caaaaacaac
//
LOCUS       DBMDWL010000221         2952 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_15505_length_2952_cov_3.751666, whole genome shotgun sequence.
ACCESSION   DBMDWL010000221 DBMDWL010000000
VERSION     DBMDWL010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15505_length_2952_cov_3.751666"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            336..1298
                     /gene="pfkA"
                     /locus_tag="ACZPDN_11030"
     CDS             336..1298
                     /gene="pfkA"
                     /locus_tag="ACZPDN_11030"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1299193.1"
                     /translation="MVKRIGILTSGGDSPGMNAAIRAVTRVGLNSGLEVFGIYNGYKG
                     MVEGYIEPLTRETVGDIVNRGGTILGSARLPEFKDIEVRKKAIAQLKKRGIEAVVVIG
                     GDGSYRGALSLTEMGINCIGLPGTIDNDITCTDYTIGFQTALETVVECVDKLRDTSNS
                     HHRCSIVEVMGNRCGDLALWSGIACGAEIVVTSDTGFDEQDVLDRLRDIDIIKKKKHA
                     IVVICEKITDVDQFAKKVSLNTGFSGRATVLGHIQRGGSPCPFDRLLASQMGEKAVDL
                     LMQGIGGQCISIRDNKIISYPIEEALSMPQESRKPLMNLFDRLV"
     gene            1325..2737
                     /gene="pyk"
                     /locus_tag="ACZPDN_11035"
     CDS             1325..2737
                     /gene="pyk"
                     /locus_tag="ACZPDN_11035"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR1299194.1"
                     /translation="MSKKTKIICTIGPASESKEVLTKLVEAGMNIARLNFSHGDYEEH
                     AERVKRIREVSKETGKPIGILLDTKGPEIRLGDFENGGCEFAEGDEIDLVKEEVLGNH
                     ERFTLRCPEVFNDVQVGDYILMDDGKMKVTFTEVTPDRIHGVVNNPHFLKSRKGCNLP
                     GIKLSMPFISEKDEADIRFGCKMDVDYIAASFTRRKDDVLAIRQILIDENKDMIQIFP
                     KIENQEGFDNIREILEVADGVMVARGDLGVDVSLELVPIYQKRMIAVANEVGKPAITA
                     THMLESMQENPRPTRAEASDVANAIMDGTDCIMLSGESAAGHYPVECVQTMTKIANAI
                     EPMIPYKDRLKANIKSSKRTLNDAIGISVADTALAIDIKCIIAFTQSGNTVRRLAKFR
                     PCAPILGITFDEVTQRSLLPVNGVTPVVSNIQNTKENDIDLARNLALQAGFKTGDHII
                     VVAGYPVGSGNTNMMRIAQI"
BASE COUNT          992 a          434 c          592 g          934 t
ORIGIN      
        1 aactcagaag ttaagcactt tcacggcgac aatagttggg cttgcccctg cgaagatagc
       61 tagctgccgg gtcctttttt ttgattttta tattcaaaag ctaagtcttt atttacttag
      121 acttggcttt tttattgcat aaataattag atatatctta aaataacagc acatgttttt
      181 agtaattgtt aacaaaatgt caaatatttc acaaatgtta ataaagttat aattcttttt
      241 acctaaaagc gttttcaggt gaattttccc ttttttaaac attcgttatg tggtatattt
      301 atgatgatta ataatgagtc tatggaggac atattatggt taaaagaatt ggtattttga
      361 cttctggtgg tgattcaccg ggaatgaatg ctgcaatacg cgcggttaca cgcgttggat
      421 taaatagtgg cctagaagta tttggtattt acaatggtta taaaggaatg gtagaaggat
      481 atattgaacc attaactcgt gaaactgttg gagatatagt aaatagaggc ggtactattt
      541 taggatcagc ccgtttacct gagttcaaag acatcgaagt cagaaaaaaa gcaattgctc
      601 aattaaagaa aagaggcatt gaagccgttg tcgttatcgg tggagatggt tcatatcgtg
      661 gtgcattatc tttaactgaa atgggaatta actgtatcgg attgcctggt actatcgata
      721 acgatattac ttgtactgat tacacaattg gattccaaac agcattagaa actgttgttg
      781 aatgcgtcga taaattaaga gatacatcga attcacatca ccgttgttct attgttgaag
      841 ttatgggaaa tcgttgtgga gacctagcat tatggtctgg tattgcctgt ggagcagaaa
      901 ttgtagtaac aagtgatact ggatttgatg aacaagatgt tttagatcgt ctaagagata
      961 tcgatattat caaaaagaaa aagcatgcaa ttgtagtaat ttgtgaaaag atcaccgatg
     1021 tcgatcaatt tgcaaagaaa gtaagtttga atacaggttt ttcaggtagg gctactgtat
     1081 taggccatat tcaacgtgga ggatctccat gtccatttga tcgtttgtta gcatcacaaa
     1141 tgggcgaaaa agccgtagat ttattgatgc aaggtattgg cggacaatgt atctcaatta
     1201 gagacaataa aattatttcg tatccaattg aagaagcatt aagcatgcca caagaatctc
     1261 gtaaaccact aatgaactta tttgatcgtt tggtttagga taaattaaaa ggaaggacaa
     1321 aaaaatgagc aaaaaaacaa aaattatttg tactattggt ccagctagtg aaagtaaaga
     1381 agttttaaca aaacttgttg aagctggaat gaacattgca cgtttgaatt tctctcatgg
     1441 agactatgaa gaacatgcag aacgtgttaa acgtattcgt gaagttagta aagaaacagg
     1501 aaaacctatc ggaattttat tagatactaa aggtccagaa attcgtttag gtgattttga
     1561 aaacggtgga tgtgaatttg ccgaaggtga tgaaattgat ttagttaaag aagaagtttt
     1621 aggaaaccac gaaagattca cattacgttg tccagaagta ttcaatgacg tacaagttgg
     1681 tgactacatt ttaatggatg atggtaaaat gaaagttact tttacagaag taactcctga
     1741 tcgtatccat ggtgtagtaa ataacccaca tttcttaaag agccgtaagg gatgtaactt
     1801 gccaggcatt aaattaagta tgccatttat ttctgaaaaa gatgaagctg acattcgttt
     1861 tggatgtaaa atggatgttg actatatcgc agcaagtttt acacgtcgta aagatgatgt
     1921 tttagcaatt cgtcaaattt taattgatga aaataaggat atgattcaaa tcttccctaa
     1981 aatcgaaaac caggaaggtt ttgataacat ccgcgaaatc ttagaggttg cagatggggt
     2041 aatggttgct cgtggagatt taggtgtaga cgtaagttta gaattagtac ctatttatca
     2101 aaaacgcatg attgctgttg caaacgaagt tggtaaacca gctatcactg caacacacat
     2161 gttagaatct atgcaggaaa atcctcgtcc aacacgtgca gaagcaagtg atgtagctaa
     2221 cgctattatg gatggaacag attgtatcat gttatctggt gaatcagctg caggtcatta
     2281 cccagttgaa tgtgttcaaa caatgactaa gattgcgaac gctattgaac caatgatccc
     2341 ttataaagat cgtttaaaag caaatattaa atcaagcaaa cgtactctaa atgatgccat
     2401 tggtatttca gtagctgata ctgcattggc aattgatatt aaatgtatta ttgcatttac
     2461 acaaagtgga aatacagttc gtcgtttagc taaattccgt ccatgtgctc ctattttagg
     2521 tattacattt gacgaagtta cgcaacgcag cttattacca gttaatggtg taacgccagt
     2581 tgtttcaaat attcaaaata ctaaagaaaa tgacattgat ttagctcgta acttagcatt
     2641 acaagctgga ttcaaaacag gagatcacat tattgttgtt gctggctatc cggtaggatc
     2701 aggtaacaca aatatgatgc gtattgcaca aatctagtta acattgccct cattttgagg
     2761 gcttttttta taaaaaaaag ctgtgggtta ttccacagct tttaatgatt caaccatttg
     2821 aatgttattt agttttttat gcatacacaa attgataatt gcattgaaac caattgtcat
     2881 tacaaaacta atcacataag atattgaatt gattgttcta ccaaacataa tatcatccat
     2941 ctcggccaag tt
//
LOCUS       DBMDWL010000222         2938 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_15610_length_2938_cov_4.229115, whole genome shotgun sequence.
ACCESSION   DBMDWL010000222 DBMDWL010000000
VERSION     DBMDWL010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15610_length_2938_cov_4.229115"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(61..549)
                     /locus_tag="ACZPDN_11040"
     CDS             complement(61..549)
                     /locus_tag="ACZPDN_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299195.1"
                     /translation="MRRLIERLSPNEGDYSQSGLTNKRLIERIADEFETELVKASTDD
                     KILFPMSFTILLHPNDYSKISQYFAPVTQSIVQKFYKIIEKHRGKSFIEKCIGKCFKK
                     TNSYKEITPIGYNWEFILTKTIVSNVGGFEVGIDNPAFIISATARVYGRGCRLILRIQ
                     YL"
     gene            complement(551..1297)
                     /locus_tag="ACZPDN_11045"
     CDS             complement(551..1297)
                     /locus_tag="ACZPDN_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299196.1"
                     /translation="MNIFSSNNSISFEANLQEQIAGTLKKLYKGKNVSLSDYSLFIGI
                     TDTNVYNSLINSDFSADFQEFVSNDYGYALRKVEIVDYIKKGWTKVDEDVFIYIFGCN
                     QTAPITKSIYAEISIYKNTGSLLEKAYRLSSNENKIYFIGRGDEKRENDIIIENSPTS
                     HFLEMNKYVRSAHAHITYQKKEGFLLWADRDGTAAFGSRTRIYRKGEQTPIDLTNENV
                     PQLLCDDDKIELGKHVILLFKIVESKRIKE"
     gene            complement(1300..2445)
                     /locus_tag="ACZPDN_11050"
     CDS             complement(1300..2445)
                     /locus_tag="ACZPDN_11050"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR1299197.1"
                     /translation="MAFLGEYTSLGSLGSGGFAEVFKVRHNQLGYVRAIRVLSQIIDS
                     EKSDIYQKFIQECKVLLRLGNGNHPNIVHIYKPYLKEQKAFVEMDYIDGYNIFDYVTK
                     NDGFIEIDEVLQFTKEISSALSYCHHDIFEYCMDADSDNLQTDPNDGSRFIIDKKKED
                     ELVQKYKVIHNDMHSGNVMRRKNGGYVLLDFGLSIEGDSVAVSSRRTNGAIEFKAPEK
                     WDKDIITEQSDIYGFGCILYMMLTGVPPFPANEPDSFSTQKKLYEAHKYKTPPAIFAQ
                     RKASFENKFHKPYQQDYPNWLEEMVMKCLAKNPNDRYLNGKELHDIFINNYIEYGKTM
                     ANGLVFDDAKYYEMQLTIAKLTTALKNSQQEVRCLLESLRRIKRGKF"
     gene            complement(2448..>2938)
                     /locus_tag="ACZPDN_11055"
     CDS             complement(2448..>2938)
                     /locus_tag="ACZPDN_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299198.1"
                     /translation="FCQDKRTLGIVRMLWMAQQPMFNHLSNIGEYYNEKKKLDSFYPY
                     SSIPYIDKQNISAVSILSSYMEKQADKFDIGKPYKNTIGGKTGTPERVFVQNGKTAKK
                     NDAWYICYIKDCNVNGERHNLAVAVRMERINSGLSGRAMNLVSDVILPQLAKFNYIQY
                     RN"
BASE COUNT          859 a          561 c          429 g         1089 t
ORIGIN      
        1 aattacgata aaactccgaa aataagcaga cgatggcaac ctcggaaggc ataccctcgt
       61 ctatagatat tgaattctta gaattaaacg acaaccccta ccatataccc ttgcggttgc
      121 cgaaatgata aatgcaggat tgtctattcc cacctcgaag cccccaacat tacttactat
      181 tgtcttggta agaataaatt cccaattata accgattgga gttatttctt tatagctatt
      241 tgtcttttta aaacatttac caatgcattt ttctataaaa gacttccctc tatgcttttc
      301 tataattttg tagaattttt gtactatact ctgtgttact ggtgcaaaat attgacttat
      361 tttagaatag tcatttggat gaagtaaaat tgtaaacgac ataggaaata gaattttgtc
      421 atctgttgaa gctttgacta attctgtttc gaattcgtct gctattcgtt ctatcagtct
      481 tttgttggtc aatcctgact gactataatc tccttcattt ggacttaatc gttctattaa
      541 acgtctcatc ttattctttt attcttttag attcaactat tttaaataaa agtattacat
      601 gtttccctag ttcaattttg tcatcatcgc ataacagttg tggtacattt tcatttgtta
      661 agtcaatagg tgtttgttcc ccctttctgt atattcttgt tctagaacca aaagctgccg
      721 ttccgtcacg atctgcccat aacaagaaac cctctttttt ttgataggtt atatgggcat
      781 gagcacttct tacgtactta ttcatttcca aaaaatgcga agttggagag ttctctataa
      841 ttatatcatt ctctctcttc tcatcacctc tacctatgaa gtatatttta ttttcattgg
      901 atgataaacg atacgctttt tccaataaac tgcctgtatt cttgtaaatg ctaatttctg
      961 catatatact cttagttata ggggcagtct ggttacagcc aaatatatag atgaaaacat
     1021 cttcatcaac ttttgtccaa cctttcttta tatagtcaac tatttcaacc ttgcgtaaag
     1081 catatccata atcattactt acaaactctt ggaagtctgc tgaaaaatcg gaattaatta
     1141 ggctgttata tacattagtg tcagtaattc caatgaaaag gctatagtca gatagagaga
     1201 catttttacc tttgtataac ttttttaatg tccctgcaat ttgttcttgc aaatttgcct
     1261 caaaactgat gctgttattt gaactgaata tattcatgtt tagaattttc ctctttttat
     1321 acgtcttaaa gattcaagaa gacatctaac ctcttgttgg gagtttttaa gagcagttgt
     1381 taattttgct atggttagtt gcatttcata atatttggca tcatcaaata caagtccatt
     1441 tgccattgtt ttaccatatt caatatagtt atttataaaa atatcatgaa gttctttgcc
     1501 atttaaatat ctgtcattcg gattcttagc caaacacttc ataaccattt cttccaacca
     1561 attaggatag tcttgttggt aaggtttgtg aaatttattc tcaaatgaag cctttcgttg
     1621 tgcaaaaata gctggtggtg ttttatattt atgtgcctca tatagtttct tttgggtaga
     1681 aaagctatct ggctcatttg cagggaacgg tggaacgcct gtcaacatca tgtatagtat
     1741 gcaaccaaat ccatatatat cagattgctc cgtaataatg tctttatccc atttctctgg
     1801 agccttaaat tcaattgcgc catttgttcg tctgctgcta acagcaacac tatctccttc
     1861 aatggataat ccaaagtcca ataatacata gcctccattc tttcttcgca tcacattacc
     1921 tgaatgcata tcattatgaa tgaccttgta tttttgaaca agttcgtctt ctttcttttt
     1981 gtctattata aagcgagaac catcatttgg atctgtttga agattgtcag agtctgcgtc
     2041 catgcagtat tcgaatatgt catgatggca atatgataat gcacttgata tttccttcgt
     2101 aaattggaga acttcatcaa tctctataaa accatcattt ttagtaacat agtcaaaaat
     2161 attataccca tctatgtaat ccatttcaac gaacgctttc tgctctttta aataaggctt
     2221 atatatgtgt actatattag gatgattacc atttcctaat ctaagaagca ccttacattc
     2281 ctgaataaat ttttggtata tgtctgattt ttccgagtca ataatttgac ttaacactct
     2341 tatagctcgg acatatccta attgattatg tctaacctta aaaacctcag caaaacctcc
     2401 agagccgaga ctaccaagac ttgtatattc tcctaaaaat gccataatta attcctatat
     2461 tgaatataat taaattttgc gagttgtggt aatatcacat ctgagacaag attcatagct
     2521 cgtccagaaa gccccgaatt aatcctttcc attcgaactg ccactgcaag attatgacgt
     2581 tctccattta cattacaatc ttttatgtaa cagatgtacc atgcatcgtt tttctttgca
     2641 gtttttccat tttgaacaaa tactctttca ggagtacctg ttttaccacc aattgtattt
     2701 ttatatggtt ttcctatatc aaatttgtct gcttgttttt ccatatatga agacaaaata
     2761 gatactgcgg atatattctg tttatctata tatgggatag aggaataagg ataaaagcta
     2821 tcaagcttct ttttctcatt atagtactcc ccaatattcg acaagtggtt gaacattggt
     2881 tgctgcgcca tccagagcat gcggacaatc ccgagcgttc tcttgtcttg gcaaaatc
//
LOCUS       DBMDWL010000223         2855 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_16276_length_2855_cov_2.120552, whole genome shotgun sequence.
ACCESSION   DBMDWL010000223 DBMDWL010000000
VERSION     DBMDWL010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16276_length_2855_cov_2.120552"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="ACZPDN_11060"
     CDS             complement(<1..404)
                     /locus_tag="ACZPDN_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1299199.1"
                     /translation="MKQINFIFTKNGFHIDETKEENTSKWAESFKKDKYLALYELGFE
                     NNLKGLTPSAFYLYQLSLKFIELLSNRPELEVAREDTKVEASSEDLEYLMSIIPFAIG
                     TEFIDEKWIQNIFQHLNSQFRWDMKSYKCTVQ"
     gene            686..1378
                     /locus_tag="ACZPDN_11065"
     CDS             686..1378
                     /locus_tag="ACZPDN_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HIR1299200.1"
                     /translation="MDNKIILYHGSEKIIENPIFGAGKKNNDFGLGFYCTESNDLAKE
                     WAVSSLNDGYSNKYTLDTEYLKILNLNSSDYTILNWIAILVEHRLFSIKTPVARRAKQ
                     YLIDYFSINVNAYDLIIGYRADDSYFDYAESFLNNTISVEQLAQAMRLGKLGEQIVIK
                     SQFAFSNLKFEGYEVADKNQYYDLRKVRNDEANQYYLKMLDQEMDRLFIQDIIRGGIT
                     NDDPRIPRNICK"
     gene            1341..1982
                     /locus_tag="ACZPDN_11070"
     CDS             1341..1982
                     /locus_tag="ACZPDN_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1299201.1"
                     /translation="MMIHAYQEIYVNNAQTMLGDAFDYAINTCHISGDDFVKMFVVSS
                     FSERIQNGEPACVAGKSGIELVHEIVFETMQKELNIESEVNYSRSCEYWIGWAVAYYQ
                     WYSDRSFKEIFNVLSFEDLKRMYYTLHEADISKFVDIVDSKMKEYYVETNLKRIRTTY
                     GYTQQELSNLSGVSLRSIQLYEQRNKDINKASVDKLYRISKVFGCKIEDLIEK"
     gene            2467..2715
                     /locus_tag="ACZPDN_11075"
     CDS             2467..2715
                     /locus_tag="ACZPDN_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299202.1"
                     /translation="MNNKIIKFMNGDLQIDVTVSPNEETVWLNRNQLATLFDRDVKTI
                     GKHINNALKEELDSSTVAKFATVQKEGSRNLTTPHRKQ"
BASE COUNT          951 a          373 c          530 g         1001 t
ORIGIN      
        1 atttgaactg tacacttgta tgatttcatg tcccacctaa actgagaatt taagtgctga
       61 aatatatttt gaatccactt ttcatcaatg aattcagtac caatcgcaaa aggaataata
      121 gacattaaat attctaaatc ttcgcttgat gcttctactt tcgtatcttc tctagccact
      181 tctagttcag gacgattact taaaagttca ataaatttca aagatagttg atacaaataa
      241 aaagcgctag gagtcaatcc ctttaggttg ttttcaaacc ctaattcata gagtgccaaa
      301 tatttgtctt ttttgaatga ttcagcccat tttgaagtat tctcttcttt tgtttcatca
      361 atgtggaacc cgttcttggt aaatataaaa ttgatttgtt tcatacactg tgtatccttc
      421 gttttattat ttttctacat tttatcatat atattaaaac aattgataaa catgtctatt
      481 atgctttaaa gaatttgtaa tatgtgtcaa tggaattctt tcttccaatt tcgttcaaat
      541 ttcgttttct ctgattcgat aaggattaga gtgattgatg attttgaatt agaagaattc
      601 acaaaaaatg agtgttgtaa tttgttttca ttgtttcctt actatcacta cagtgatata
      661 attggcttga aggagataaa tgttaatgga taataaaata attttgtacc atggatctga
      721 aaaaataata gagaatccaa tatttggtgc tggtaaaaag aataatgatt ttgggttggg
      781 attttattgt acagaaagta atgatttggc aaaagaatgg gcagtatcat ctttaaatga
      841 tggttattct aataagtata ctttagatac agagtatttg aaaattttga atttaaatag
      901 ttcggattat acgattttaa attggattgc gatattggta gagcatcgtc ttttttcgat
      961 taaaacgcct gtggctagac gagcaaaaca atatttgatt gattatttta gtatcaatgt
     1021 gaatgcatat gacttgatta taggctatcg tgcggatgat tcttattttg attatgcaga
     1081 aagtttcttg aataatacaa tttcagttga gcaactcgca caagctatga gattgggcaa
     1141 attaggagaa cagattgtca ttaagtcgca gtttgcgttt tcaaatttaa agtttgaagg
     1201 gtatgaggtt gctgataaaa atcaatatta tgatttacgt aaagtaagaa atgatgaagc
     1261 aaaccaatat tatttgaaaa tgcttgatca agaaatggat agactgttta ttcaagatat
     1321 tataagaggg ggcattacaa atgatgatcc acgcatacca agaaatatat gtaaataatg
     1381 cacaaacaat gttaggagat gcctttgatt atgccatcaa tacttgtcat atttcgggtg
     1441 atgattttgt gaagatgttt gtcgtaagtt catttagtga acgtattcaa aatggggagc
     1501 ctgcgtgtgt ggcgggtaaa agtggcatcg agcttgtaca tgaaattgtg tttgagacca
     1561 tgcaaaaaga gttgaatatt gaatctgaag taaactatag tcgttcatgt gagtattgga
     1621 ttggttgggc tgttgcctat taccaatggt attcagacag aagttttaaa gagatattta
     1681 atgttttaag ttttgaagat ttgaaaagaa tgtattatac acttcatgag gcggatattt
     1741 ctaagtttgt agatattgtg gattctaaaa tgaaagagta ttatgtggaa acaaacttaa
     1801 agcgtattcg aactacttat ggttatactc aacaagaatt atcgaatctt tctggtgtaa
     1861 gtcttcgttc aattcagttg tatgaacaaa gaaataaaga tattaataag gcgagtgtag
     1921 ataagttgta tcgtatttct aaagtatttg gttgtaagat agaggattta attgaaaaat
     1981 aatatacgag gatagaattc aatgtcgttc tatccttttt ttgaataatt tagtagaatt
     2041 cacaatgttt tcacattggc cttttataat aggaattggc tattggatat agtagggtgg
     2101 tacctctgaa attaaaagta atttagcttg agttctcggc tcgattccga ttattgtagg
     2161 aggttgtcat atgtacattt atctttatat tgacgttgcc atgtgtttaa tggctttagt
     2221 gcaaatacta cgacttacgt aaatgattat cgaaaaatgc aaaagacact agtccacctt
     2281 tcccgcaaag aagttaaact agtgccttag cattagaatg atttcagagc agccactgta
     2341 tccagtagct tttctctatt tgtattatac cgataaagga atggttatgc aaataatata
     2401 gagtttgtta attaatgaag aaaataatta tctggatcgt ttaaataaag aaagattgga
     2461 gtacatatga acaataaaat aattaagttt atgaatggtg atttacagat tgatgtaacg
     2521 gtttcgccaa atgaagaaac tgtctggtta aatcgaaatc aattagcaac cttatttgac
     2581 agagacgtta aaacaatcgg taagcacatt aataatgcat taaaagaaga acttgattct
     2641 tcaactgtcg caaaatttgc gacagttcaa aaagaaggct ctagaaatct gactactcct
     2701 caccgcaagc agtgagggtt ctgaactgac aacattatgc agtaatcgta cattattttc
     2761 aaactttgga ttcctgtctt tctgttggag cagtgaactc tacaatcaag cactacattt
     2821 agcacattag cgattgtttt ctgtatctct acatg
//
LOCUS       DBMDWL010000224         2840 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_16412_length_2840_cov_2.133991, whole genome shotgun sequence.
ACCESSION   DBMDWL010000224 DBMDWL010000000
VERSION     DBMDWL010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16412_length_2840_cov_2.133991"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            705..1073
                     /locus_tag="ACZPDN_11080"
     CDS             705..1073
                     /locus_tag="ACZPDN_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1299203.1"
                     /translation="MIIINNSSMIPIYEQIVNQVKQNIVDGKLKEGDALPSVRGLSKD
                     LKISALTVKKAYDILEADGLCRTVQGKGSFVLGVNPGLVHEQRVVEIQKDLEKILDKA
                     KSYDLSKDEIRDLFDILMEE"
     gene            1078..1857
                     /locus_tag="ACZPDN_11085"
     CDS             1078..1857
                     /locus_tag="ACZPDN_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1299204.1"
                     /translation="MRFVKTYNDFKLDCKLELKPGTITALIGRNGSGKTTLFKLYLGL
                     IEDDDKHTNQVNKEEIGVVWNDSSFSLVLNVLEIKKILMYSYKHFDCDYFDSMVKNFE
                     LDPKKPLKEYSTGMLSKLKIIIATSLHAKILLLDEPTSGLDVIARNEVLDLLRDYMEQ
                     NEEVSILISSHISSDLEGLCDEFYFIEKGKIILQEQDLESYALLKGVDETVDLSYALK
                     KIGSTYLTNERQFYVENYPGLIIEKARIDDLMEYMIKGETV"
     gene            1917..>1964
                     /locus_tag="ACZPDN_11090"
     CDS             1917..>1964
                     /locus_tag="ACZPDN_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299205.1"
                     /translation="MLFIGIIGGINDISFA"
     assembly_gap    1965..1974
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1975..2420
                     /locus_tag="ACZPDN_11095"
     CDS             <1975..2420
                     /locus_tag="ACZPDN_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR1299206.1"
                     /translation="ANHGLKTLFTLPISKSDYVKEKYLFSLIITGIGFVLVTILSCFS
                     KSGFMETLIILSTALLLLALSLPFQLKEGNEKGRIVLFVVVFGCTFLFAFLNQFIPKF
                     FESIESLLNTLDPIMFSVGLLITSFILYFISMRISIHVYNKRILD"
     gene            complement(2417..>2840)
                     /locus_tag="ACZPDN_11100"
     CDS             complement(2417..>2840)
                     /locus_tag="ACZPDN_11100"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1299207.1"
                     /translation="ISSLCEKADINRSTFYKYYGSQYDVIKEMEQEVIQLINEQLNDQ
                     PKETSFIQLLQFLKSNKETIKIFFECNVDNEFPKQVLALDSINNQLEQVSQNDPYIKN
                     YILFGSLATIQQWIDAGCIESCEEIANIINSIVDKLVR"
BASE COUNT          907 a          345 c          557 g         1021 t
ORIGIN      
        1 tcggatttga atctatgccc aattaacaac cgtcagggaa ttgtaattga cggtgaaggt
       61 tcaaaggtta tttgtaaaga ctaatttgaa aattccagtt tgtcgatgta taagacaaaa
      121 ctagtaacgg gtaaacctca tcaatttacc cgcttttttt tatgccttct atttaactat
      181 ggggagtatt ttggaggttt ccgtgaggat ggtttgtcca agttgtaaaa agagaagacc
      241 ttcatttaaa gcacctgcat ctaagaatat ctgtgattgc atcccgaacg cctaagaaca
      301 aagagactta atgtttcttt gtcttattgt gctttattga gcacgaataa gtaaatgtaa
      361 ttcttagttt catgaaaaca ttgacaaaaa tgactaaatt aaaccagata atggctaaat
      421 tgaaacgact ttttagtcta atcctagact acgttacccg tttttagttt taccgtttca
      481 agtgagtatt acccgttctt actttaacgg tttgtgttct gcctcgctaa atgcacggct
      541 tagtttaaca tcgacctgcg ggcagaattt gctaaatgca cggcttagtt tctatatgca
      601 cgttatagtt taacgatgac atttgaatta ggatagtttt atcctaattt ttttctttac
      661 aagtgatata tactgttata cactgttata tagaaggtga tacaatgatt attatcaata
      721 atagttcaat gattcctatt tatgaacaga ttgtgaatca agtgaaacaa aatattgtgg
      781 atggtaagtt aaaagaagga gatgctttac cgagtgttcg tggtctatca aaagatttaa
      841 aaatatcggc tttaactgtt aaaaaagcat atgacatatt agaagcggat ggtttatgta
      901 gaacggttca aggtaagggt tcgtttgtgt tgggggtaaa tcctggcttg gtacatgagc
      961 agcgtgtagt tgaaattcaa aaggatttag agaagatact ggataaagcg aaatcttatg
     1021 atttatcaaa ggatgaaatt agagatttat ttgatatctt gatggaggaa taagaatatg
     1081 cgatttgtaa agacatataa tgattttaaa ttggattgca agcttgaatt aaagccagga
     1141 acaataacgg ctttgattgg tcgtaatggc tcgggtaaga ccactttgtt taaattgtat
     1201 cttggtttaa ttgaagatga tgataaacat acaaatcaag tgaataaaga agaaattggt
     1261 gttgtatgga atgattcgag ttttagttta gttttaaatg ttcttgaaat taaaaagatt
     1321 ttaatgtatt cctataaaca ttttgattgt gattattttg attctatggt aaaaaatttc
     1381 gaattggatc caaagaaacc attaaaagaa tattctacgg gtatgctttc aaaactgaag
     1441 attattattg cgacaagtct tcatgcgaaa atattattgt tggatgaacc aacgagtggt
     1501 ttagatgtga ttgcacgaaa tgaagtattg gatttgttgc gggattatat ggaacaaaat
     1561 gaagaggttt ccatattgat tagttcacat atctcaagtg atttggaggg tttgtgtgat
     1621 gaattttact ttattgaaaa aggaaaaatc attctgcaag aacaggatct agaatcttat
     1681 gcactattaa agggtgttga tgaaactgtg gatttaagct atgcattaaa aaagataggt
     1741 tcgacttatc ttacaaatga aagacaattc tatgtagaaa actatccagg cctaattatt
     1801 gaaaaagcac gtattgatga tttgatggaa tatatgatca agggggaaac tgtatgaagg
     1861 gattgttagt caaagatttc ttgttgatga taaaaagtaa gaaagtcata ttgtttatgt
     1921 tgttcattgg aatcattggt gggataaatg atatttcatt tgcgnnnnnn nnnnaagcca
     1981 atcacggctt aaagactttg tttactttgc ctatttctaa atcggattat gtgaaagaaa
     2041 aatatttgtt ttctttgatt attacgggaa ttgggtttgt gttagtcaca atattaagtt
     2101 gttttagtaa aagtggtttc atggaaacac ttataatttt atcaaccgct ttgttgttgt
     2161 tagctttatc tttgcctttt caattaaagg aaggtaatga aaaaggaaga attgtattgt
     2221 ttgtggttgt gtttggatgt accttcttat tcgctttctt aaatcaattt attcctaaat
     2281 tctttgaatc gattgaatca ttactgaaca cattagatcc tattatgttt agtgtaggtt
     2341 tattgattac aagttttatt ctatatttta tttctatgag gatttcaatt catgtataca
     2401 ataagcgaat tcttgattaa cgtactaatt tatctacaat actgttgatg atattcgcaa
     2461 tttcttcaca agattcaata catcctgcat cgatccattg ttgaatggtt gccaaagatc
     2521 caaaaaggat gtaatttttg atgtatggat cgttttgtga gacttgttca agctgattgt
     2581 tgatggaatc gagtgctaga acttgttttg gaaattcatt gtcgacattg cattcaaaaa
     2641 agattttgat ggtttctttg ttggatttta aaaattggag taattggata aaagaagttt
     2701 cttttggttg atcatttaat tgttcattga tcaattgaat gacttcttgt tccatttctt
     2761 taatgacatc atattgactg ccataatact tgtagaaggt ggaacgattg atatcggcct
     2821 tttcacataa gctagaaatg
//
LOCUS       DBMDWL010000225         2799 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_16744_length_2799_cov_2.704596, whole genome shotgun sequence.
ACCESSION   DBMDWL010000225 DBMDWL010000000
VERSION     DBMDWL010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16744_length_2799_cov_2.704596"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="ACZPDN_11105"
     CDS             complement(<1..369)
                     /locus_tag="ACZPDN_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1299208.1"
                     /translation="MQGYKTHVQSGILLNANEASQNVNETVKQEIIDAISNLSLNRYP
                     DTTCHQLHRLYAEVMNVPPSWILSGNGSDQMLGFLIQYYLQENKILYTLSPDFSMYDY
                     YVGLNHSKIEKYETNQDGSFD"
     gene            complement(369..1649)
                     /gene="hisD"
                     /locus_tag="ACZPDN_11110"
     CDS             complement(369..1649)
                     /gene="hisD"
                     /locus_tag="ACZPDN_11110"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR1299209.1"
                     /translation="MLTRIYKEDKDVLKEYLMSRTQDLNTDVLVTVSNIIQDVKNNKD
                     EACLKYTREFDHVLLDTMEVTEEELDIQISKCDKDFVEAMKLAKKNIEDFHKLQKQNG
                     YLLQKEMGIYLGQRVLPLEAVGIYVPGGRAQYPSSVLMNALPAKIAGVKNVVMVTPPD
                     SEGTIHPNIAYAAKLAGVDKIYKIGGAQAIAALAYGTQTIDRVDKIVGPGNIFVAAAK
                     KLVYGTVDIDMIAGPSEILVVADENANPEYVAADLLSQAEHDPMASAILLTTSEDLLN
                     SVNQQLSKQSAILPKKEIVEQSLKNYGKAIVCDSLEECISISNEIAPEHLELMISNPM
                     EYLQEVKNAGSVFLGYYTCESIGDYFGGTNHVLPTSGTARFSSALSVDSFIKKSSYLY
                     YSKQAIETYAKYIETIASEEHLQAHANAAKVREK"
     gene            complement(1630..2277)
                     /gene="hisG"
                     /locus_tag="ACZPDN_11115"
     CDS             complement(1630..2277)
                     /gene="hisG"
                     /locus_tag="ACZPDN_11115"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR1299210.1"
                     /translation="MGLSIALTKGRLEKQTVSMLEDLGYGIEALKDKGRALVFKDSIE
                     DIQYFLVKSNDCITYVNHGVADIGVVGKDTILENENDTYELLDLKIGKCKFIVASLPQ
                     NQLFSKVGHIKIGTKYPTVAKQYFLSKGMDVEIIKIDGSVELAPILGLCDGIVDIMET
                     GTTLKENGLVVLDTVCDISARVIVNKASFKLKHSEVMKVIEDLSRKVNADVDTNL"
     gene            complement(2277..>2799)
                     /locus_tag="ACZPDN_11120"
     CDS             complement(2277..>2799)
                     /locus_tag="ACZPDN_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1299211.1"
                     /translation="LDSLYLSEKIVDFFMHLPFLCGDVNILDEAYAYCFDESLKEVLD
                     SMKECIQSLNELGCLENVTIDLGKVAHLDYYTGIIFEGYVSGVGTSILSGGRYDCLLK
                     KFGRDLPACGFSVKLDPLIDHVDVQLKKKFILYYPESKQIEAMKQANKLRKEGSVILE
                     PSLNETIYVKEVE"
BASE COUNT          878 a          561 c          361 g          999 t
ORIGIN      
        1 atcaaatgaa ccatcttgat ttgtttcata tttttcaatc tttgaatgat ttaaccctac
       61 ataatagtca tacatagaga agtctggact caaagtataa agtattttat tttcctgtaa
      121 ataatattga attaaaaatc ctaacatttg atccgaccca tttccagata agatccaaga
      181 aggcggaaca ttcataacct ctgcatataa acgatgtaat tggtggcaag ttgtatctgg
      241 ataccgattt aaggatagat ttgaaatcgc atctataatt tcttgtttaa ctgtttcatt
      301 cacattttga cttgcttcat tggcattcaa caaaatccct gactgtacat gtgttttata
      361 tccttgcatt atttttccct cacttttgcg gcatttgcgt gtgcctgtaa atgctcttca
      421 ctcgcaatag tctcaatata ttttgcgtaa gtttcaatag cttgttttga ataatataaa
      481 taagatgatt tttttataaa actatctaca gataatgcac tagaaaatcg tgcagtgcca
      541 ctggtaggta aaacatggtt ggttccccca aaatagtcac caatactttc acaagtataa
      601 tagcctaaga aaacactacc tgcattttta acttcttgta gatattccat aggatttgaa
      661 atcattaatt ctaaatgttc tggtgcaatc tcattactaa ttgaaataca ttcttcaagc
      721 gaatcacaaa caatggcctt accataattc tttaaggatt gttcaacaat ttctttttta
      781 ggtaaaattg cactttgttt tgaaagctgt tgattcacag aatttaaaag gtcttcgctt
      841 gttgtcaaaa gaatcgcact tgccatagga tcatgttcgg cttgtgataa taaatctgct
      901 gccacatatt ctggattggc attttcatcc gctacaacta agatttcact tggacctgca
      961 atcatatcaa tatcaacggt tccatatact aattttttag cagctgccac aaagatgttt
     1021 cctgggccca caatcttatc tacacgatca atcgtttgtg tgccataagc taaggccgca
     1081 atggcttggg ctccaccaat cttataaatt ttatccacac ctgcaagctt ggcggcatat
     1141 gcaatattag gatgaattgt accttctgaa tctggtgggg ttaccatcac tacatttttt
     1201 actccggcaa tttttgcagg cagtgcattc attaagacac tacttgggta ttgtgctcgt
     1261 cctccaggca cataaatacc tacagcttct aaaggaagaa ctctttgtcc taaatagatt
     1321 cccatttctt tttgaagtaa atatccattt tgtttttgaa gtttatggaa atcttcaata
     1381 ttctttttag caagtttcat agcttctaca aaatctttat cacacttaga aatctgaata
     1441 tctaactctt cttccgtcac ttccattgta tctaacaaaa catgatcaaa ctcacgagtg
     1501 tattttaaac aagcctcatc tttattattt tttacatcct gaataatatt tgatacagtc
     1561 accaatacgt ctgtatttaa atcttgtgtt cgtgacatca aatattcttt taatacatcc
     1621 ttatcttctt tataaattcg tgtcaacatc tgcattcacc ttcctactta aatcttctat
     1681 cactttcata acttctgaat gttttaattt aaaacttgcc ttatttacga tgacacgagc
     1741 actgatatca catactgtat ctaaaacaac taagccattt tccttaagtg ttgtacccgt
     1801 ttccataata tcaacaattc catcacataa acctaaaata ggagccaatt ctacagaacc
     1861 atcaattttg ataatttcca catccatacc ttttgataaa aaatattgtt tcgcaaccgt
     1921 tggatatttt gttccaatct taatgtgtcc tacttttgaa aacaactgat tttgaggtag
     1981 actagccaca ataaatttac attttccaat ttttaaatct aacaattcat aagtatcatt
     2041 ctcattttct agaattgtat ctttacctac aactccaata tcagcaactc catgattaac
     2101 ataggtaata caatcattgg atttaactaa aaaatattga atatcttcaa tagaatcttt
     2161 aaaaacaagt gcacgtccct tatcttttaa ggcttcaatt ccatatccta aatcctctaa
     2221 catagatacg gtttgttttt ctagacgtcc ttttgtcagt gcaatagata gacccatcat
     2281 tctacctcct ttacataaat tgtttcattt aaggaaggct ctaatattac acttccttct
     2341 tttcgtaact tatttgcttg tttcatagct tcaatttgtt tggattctgg ataataaagt
     2401 ataaatttct ttttaagttg cacatccaca tgatcaatca gcggatctag ttttacacta
     2461 aagccacatg ccggtaagtc tcgaccaaat tttttaagta aacaatcata tcttcctcca
     2521 cttaaaatac ttgttccaac accagataca tagccttcaa aaatgattcc tgtatagtag
     2581 tcaagatgtg ccacttttcc taagtcgatt gtcacatttt ctaaacatcc caattcattt
     2641 aaagattgaa tgcattcttt catggaatcc aagacctctt ttaaagattc atcaaaacaa
     2701 tatgcgtatg cttcatccaa gatatttaca tctccacaaa gaaatggcag gtgcataaag
     2761 aaatccacaa ttttttcact taaatataat gaatctaaa
//
LOCUS       DBMDWL010000226         2782 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_16896_length_2782_cov_4.631481, whole genome shotgun sequence.
ACCESSION   DBMDWL010000226 DBMDWL010000000
VERSION     DBMDWL010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16896_length_2782_cov_4.631481"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="ACZPDN_11125"
     CDS             complement(<1..923)
                     /locus_tag="ACZPDN_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR1299212.1"
                     /translation="MIRMGIDLGTNYIRLCTQEDGLLYNEPCMVALDNQNHVLGIGED
                     AKDMIGSTDSNVRIISPLRENPINFDALDILLEQLLYEHKAFKMFQKTILLVSYPTSF
                     SEKSCQILKEHLEDLGAYRVYFEQEIWIAAIGAKLDLFLPVASCVLNIGSSNCDIALF
                     LNGKMEKKARCNVSGITINIALKNWLTNSYNINVSNKQIEKIKRKLGQVNIQQNPKSL
                     EIVGMDKNSHVLKSIIINENQIVGVLTPLVQQWANWILQFLSNLPITTQQDVKTRGIV
                     CCGGSMLLSGLSTYLQNTIGCPIFVTDDPRSS"
     gene            1006..1461
                     /locus_tag="ACZPDN_11130"
     CDS             1006..1461
                     /locus_tag="ACZPDN_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299213.1"
                     /translation="MNNIEINNIKETALLVILDSVELEKTMSHHDLFLLWSKAFENVG
                     IPIYIVFPDDVMKLKKFYRFYNTDIHYISDVDLIERLDCVQEKVVFGKKYRVILPKMY
                     VVHDKKLVNEQKRINNKMIKKLYIRALELKFENFIKKIKNSVDIPNNLW"
     gene            1501..1576
                     /locus_tag="ACZPDN_11135"
     tRNA            1501..1576
                     /locus_tag="ACZPDN_11135"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1534..1536,aa:Val,seq:tac)
     gene            1584..1667
                     /locus_tag="ACZPDN_11140"
     tRNA            1584..1667
                     /locus_tag="ACZPDN_11140"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1618..1620,aa:Tyr,seq:gta)
     gene            1673..1748
                     /locus_tag="ACZPDN_11145"
     tRNA            1673..1748
                     /locus_tag="ACZPDN_11145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1705..1707,aa:Gln,seq:ttg)
     gene            1755..1828
                     /locus_tag="ACZPDN_11150"
     tRNA            1755..1828
                     /locus_tag="ACZPDN_11150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Gly,seq:tcc)
     gene            1859..1934
                     /locus_tag="ACZPDN_11155"
     tRNA            1859..1934
                     /locus_tag="ACZPDN_11155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1892..1894,aa:Val,seq:tac)
     gene            1943..2018
                     /locus_tag="ACZPDN_11160"
     tRNA            1943..2018
                     /locus_tag="ACZPDN_11160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1976..1978,aa:Thr,seq:tgt)
     gene            2024..2099
                     /locus_tag="ACZPDN_11165"
     tRNA            2024..2099
                     /locus_tag="ACZPDN_11165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2057..2059,aa:Lys,seq:ttt)
     gene            2110..2183
                     /locus_tag="ACZPDN_11170"
     tRNA            2110..2183
                     /locus_tag="ACZPDN_11170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2142..2144,aa:Gly,seq:tcc)
     gene            2228..2311
                     /locus_tag="ACZPDN_11175"
     tRNA            2228..2311
                     /locus_tag="ACZPDN_11175"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2262..2264,aa:Tyr,seq:gta)
     gene            2453..2671
                     /gene="infA"
                     /locus_tag="ACZPDN_11180"
     CDS             2453..2671
                     /gene="infA"
                     /locus_tag="ACZPDN_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR1299214.1"
                     /translation="MAKQDMIEIDGIVVDTLPNAAFKVKLENGHEILAHVSGKIRMHH
                     IRILPGDRVTVEISPYDLTRGRITFRRK"
     gene            2686..>2782
                     /gene="rpmJ"
                     /locus_tag="ACZPDN_11185"
     CDS             2686..>2782
                     /gene="rpmJ"
                     /locus_tag="ACZPDN_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR1299215.1"
                     /translation="MKVRPSVKPICDKCRVIRRKGRVMVICENPKH"
BASE COUNT          831 a          481 c          551 g          919 t
ORIGIN      
        1 gaagacctag gatcgtctgt gacaaagatt ggacatccta tagtattttg tagataggtt
       61 gatagtccac ttagtaacat ggatcctcca caacatacaa ttcctctcgt ttttacgtct
      121 tgttgcgttg tgattggtaa gtttgataaa aattgtagga tccaatttgc ccattgttgt
      181 acgagtgggg ttagaacgcc aacaatttga ttttcattga tgataatcga ttttaatacg
      241 tgactgtttt tatccatacc aacgatttca agactttttg gattttgttg gatattgact
      301 tggccaagtt ttcgtttaat tttttcaatt tgtttattag aaacattgat attgtatgaa
      361 ttggttagcc agtttttcaa ggctatattg attgtgatgc ctgatacgtt acaacgtgct
      421 ttcttttcca ttttgccatt taagaatagg gcaatatcac aattggatga tccaatgttc
      481 aatacgcagc ttgcgacggg taagaaaaga tcaagcttag ctccgattgc cgcaatccag
      541 atttcttgct caaaataaac acgatacgct cctaggtctt ctaaatgttc ttttagtatt
      601 tgacatgatt tttcactaaa acttgttggg tagcttacca atagaatggt tttttggaac
      661 attttaaaag ctttgtgttc atataataat tgttctaata aaatgtcgag agcatcaaag
      721 ttgattggat tctcgcgtaa aggggatatg attctaacat ttgaatctgt agaaccaatc
      781 atatctttgg cgtcttcgcc tatgcctaac acatggtttt gattatcaag cgcaaccatg
      841 catggctcat tataaagtaa tccatcttct tgggtacata agcgtatata gtttgtacct
      901 aaatctattc ccattcgaat catacgattc ctcctaataa tatatgcctt tattttacac
      961 caggttaaat tgtgatacaa tatgcatgca ttcctacgta tataaatgaa taatattgaa
     1021 attaataata taaaagaaac agcactcttg gttatattgg attctgttga attggaaaaa
     1081 acaatgtcgc atcatgactt gtttttattg tggagtaagg catttgagaa tgtgggtata
     1141 cctatatata ttgtttttcc agatgatgtt atgaagttga aaaaatttta tcggttttat
     1201 aataccgata ttcattatat ttcagatgta gatctaattg agagattaga ttgtgttcaa
     1261 gaaaaggtag tttttggaaa aaagtacaga gtaattttac cgaaaatgta tgtggttcat
     1321 gataaaaaat tagtaaatga acaaaaaaga ataaataata aaatgataaa aaagctttat
     1381 ataagggctt tagaactaaa gtttgaaaat tttataaaaa aaattaaaaa ttccgttgac
     1441 atccctaaca acctttggta atataaatga gcgcaaacga gtttgcggcg aaaaaagcat
     1501 ggaggtttag ctcagttggg agagcatctg ccttacaagc agagggtcag cggttcgagc
     1561 ccgttaacct ccaccatgtt gatggagaag tagcgaagtg gctaaacgcg gctgactgta
     1621 actcagttcc ttttggttcg gcagttcgaa tctgtccttc tccaccactt attggggtat
     1681 agccaagcgg taaggcacag gactttgact cctgcatttc cctggttcga atccaggtac
     1741 cccagccatc atttgcaggt gtagttcaat ggtagaactt cagccttcca agctgactac
     1801 gtgagttcga ttctcatcac ctgctccatt aattttatcc ctttgtatgg caagttttgg
     1861 aggtttagct cagttgggag agcatctgcc ttacaagcag agggtcagcg gttcgagtcc
     1921 gttaacctcc accatttaaa atgccgactt agctcaattg gtagagcaac tgacttgtaa
     1981 tcagtaggtt gtgggttcaa ttcctatagt cggcaccact tgtgacccgt tagctcagat
     2041 ggtagagcat ttgactttta atcaaagggt cgggcgttcg aatcgcccac gggtcaccac
     2101 ttatattatg caggtgtagt tcaatggtag aacttcagcc ttccaagctg actacgtgag
     2161 ttcgattctc atcacctgct ccattttatt aataacaatc ttcagattgt tctttttttt
     2221 aaaaattgga gaagtagcga agtggctaaa cgcggctgac tgtaactcag ttccttttgg
     2281 ttcggcagtt cgaatctgtc cttctccacc actttaaaag taacacaact taataaagtt
     2341 gtgttttttt taaattatac ttggctattt aaagcttttt tgttaaaatg gtagatggca
     2401 ttttatgcct aacatcattt cattttttga tagaaggagg agtgtaggat tcatggctaa
     2461 acaagatatg atcgaaattg atggtatcgt agttgacact ttacccaatg cagcttttaa
     2521 agttaaattg gaaaacgggc acgagattct agctcacgtt tctggtaaaa tccgtatgca
     2581 tcacattcgc attttaccag gtgatcgtgt taccgttgaa atttcaccgt atgacttaac
     2641 acgtgggcgt attacattta gacgcaaata aggagacgat taaagatgaa agtaagacca
     2701 tctgttaaac caatctgcga caagtgtcga gtaattcgac gcaaaggacg tgtgatggta
     2761 atttgtgaaa acccaaaaca ca
//
LOCUS       DBMDWL010000227         2747 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17210_length_2747_cov_1.715042, whole genome shotgun sequence.
ACCESSION   DBMDWL010000227 DBMDWL010000000
VERSION     DBMDWL010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17210_length_2747_cov_1.715042"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="ACZPDN_11190"
     CDS             complement(<1..583)
                     /locus_tag="ACZPDN_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014851.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR1299216.1"
                     /translation="MKGTRIQRSDTMRTPDLNWLKDSRIFQVNRLQAYSDHKYTIGKD
                     KKPSSFSLNGTWKFNQSKNYDTCFKFFYNTEFNDANWNDIQVPGHIQLQGFDNPHYTD
                     VTYPWDGHDVVKPGQIDMDKNKVYQYRKTFDCPQDFIGNKLILSFEGVESNIYVWLNG
                     KFIGYPPRSRQFCMFSRGICLKLSIVSPPHRFYI"
     gene            654..791
                     /locus_tag="ACZPDN_11195"
     CDS             654..791
                     /locus_tag="ACZPDN_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299217.1"
                     /translation="MSDILGLVVSIALIYFAIKFTFKCIKWIGILLIVVFVFKMVALL
                     I"
     gene            960..2045
                     /locus_tag="ACZPDN_11200"
     CDS             960..2045
                     /locus_tag="ACZPDN_11200"
                     /inference="COORDINATES: protein motif:HMM:NF017932.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HIR1299218.1"
                     /translation="MTKNKVFLSIVVFVGVFSLLYGYQDLIGTEEINMVDQALINGFD
                     FQMSFLVGLLSGLLVLVLTYNKEKIDPNILTEEFIRTKFSVSDLEKFEMLDEETKQGV
                     YDYYQDHFDLDDVADCLSYIEKKQPKTNKFVKFGLLGVICCALILVLSPVHSDYVSAK
                     EQYNEILRQQEEAYNQIITEQYLYYEGLPTIEILPGNNLKAGDVQKYVDEFIRTQPQF
                     LLDNCRLIKFCEPQNFDAIAVADGMDIDNRGFGTYAYASSSDFSITLQMDVDKDYDQK
                     GTVSHELTHIFDFAHANCYTYYGISDSYEWQKLHEMAPGSLGEYGRDDTAEFFADAGE
                     MYINHPDELKEANMDIYNFMNNLYQMY"
     gene            2134..2523
                     /locus_tag="ACZPDN_11205"
     CDS             2134..2523
                     /locus_tag="ACZPDN_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299219.1"
                     /translation="MKKVIGLLVMLVCFTGCASNGGVDYEDPSTFQEQNFASYEEWVD
                     AELDLAQYYGGFTDDQLAYFREVAMQDEALFREEFRRFNEEVESYLQQQDMIVQQQNE
                     MANQQQQQMFEQQQQMFDQQVQMSMGF"
     gene            2539..>2747
                     /locus_tag="ACZPDN_11210"
     CDS             2539..>2747
                     /locus_tag="ACZPDN_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299220.1"
                     /translation="MQHELDWLSRYLDKKGIDLIVLYQLSVKEIIKYLRLSPFQRKCF
                     NGIYCSCLSDFTYKELLLIVKRNVW"
BASE COUNT          885 a          305 c          588 g          969 t
ORIGIN      
        1 agatatagaa tctatgagga ggactgacta tggaaagctt cagacatatg cctcttgaaa
       61 acatacagaa ctgccgcgac ctcggcggat atccgataaa tttaccattt agccatacgt
      121 agatattgga ttctacacct tcaaaggaaa gaatgagttt attgccaatg aagtcttgtg
      181 gacaatcgaa tgttttacgg tattgataga ctttgttttt gtccatgtca atttggccgg
      241 gtttgactac gtcatgtcca tcccatgggt aggtaacgtc tgtatagtgt ggattgtcaa
      301 atccttgaag ttgaatgtgt ccgggaactt gtatgtcgtt ccaattggca tcattaaatt
      361 ctgtgttata gaagaattta aagcatgtat catagttttt ggattggttg aatttccatg
      421 ttccatttag ggaaaaagag cttggttttt tgtcttttcc aattgtatat ttatgatcac
      481 tgtatgcttg tagtcgattg acttgaaaga tacgtgagtc ttttagccag tttaaatctg
      541 gtgttcgcat tgtatcactc ctctgtattc gtgtaccttt cattatagca aaccgcccta
      601 ttgaaaggaa ggtgaaaatg aattaaaata ggtgttaaat tggaggttga aagatgtcag
      661 acattcttgg tttagtggtt tcaatagctt tgatttattt tgcgattaag tttactttta
      721 aatgcattaa atggattggt attcttttga ttgtagtctt tgtgtttaag atggtagcac
      781 ttcttatata agggaacatg gagttctctt tttttttatg ttctaacgat tatttatagt
      841 tttaacgcaa taattagaaa ataatttaat tttttttatt ttttatataa ccgattctaa
      901 atttacttcg tcttaatagt gtaaggataa aaaaacaaag taagaaggag agatttagta
      961 tgactaaaaa taaagtattt ttatcgattg tagtatttgt aggtgtattt tcattgttgt
     1021 atggatatca agatttaatt gggactgaag aaattaatat ggttgatcag gcgcttatta
     1081 atggttttga ttttcaaatg tcattcttgg ttggattatt gagtggattg ttggtgttgg
     1141 ttttgactta taacaaggaa aagatagatc ctaatatttt aacggaggaa tttattcgta
     1201 caaagttttc agtgtctgat ttagagaagt ttgaaatgtt ggatgaagaa acaaaacagg
     1261 gtgtctatga ttattatcaa gatcattttg atttggatga tgtagcggat tgtttgagtt
     1321 atattgaaaa gaaacagcct aagacaaata agtttgtgaa atttggattg ttgggagtca
     1381 tttgttgtgc attgatttta gttttgagtc cggttcactc agattatgta tcggctaagg
     1441 agcaatataa tgagattctt agacagcagg aagaggcata caaccagatt attacagaac
     1501 aatatttata ttatgagggt ttacctacaa ttgagattct tcctggtaat aatttaaaag
     1561 ctggagacgt tcagaaatat gtggatgaat ttattagaac gcagccgcag ttcttattgg
     1621 ataattgtag attgattaaa ttttgtgaac ctcaaaattt tgatgcgatt gcagttgcgg
     1681 atggtatgga tattgataat agaggatttg gaacctatgc atatgcatct tctagtgatt
     1741 tttcaattac tttacaaatg gatgtagata aagattatga tcaaaaaggg acagtgagtc
     1801 atgagttaac acatatcttt gattttgcac atgcgaattg ttatacatat tacggtattt
     1861 ctgatagtta tgaatggcaa aaattacatg aaatggctcc tggaagttta ggagagtatg
     1921 gtagagatga tacggcagaa ttctttgcgg atgcaggtga aatgtatatc aatcatccag
     1981 atgaattgaa agaagcaaat atggatattt acaattttat gaataatttg tatcagatgt
     2041 attaaaaaaa taaaaatatt tatattttta tacaaccaat tgaagaatga cttcgtctta
     2101 tatagtgaag aggtaaataa ggaggaaatg aaaatgaaaa aggttatcgg tttattagtg
     2161 atgttagtgt gttttacagg gtgtgcaagt aatggtggtg ttgattatga agatccatct
     2221 acatttcaag aacaaaattt tgcgtcatat gaagaatggg tagatgcaga attggattta
     2281 gcacaatact atggaggttt cacagatgat caactggcct attttagaga ggttgctatg
     2341 caagatgaag cgttgtttag agaagaattt cgtcgtttta atgaagaggt tgaatcttat
     2401 ttgcagcaac aagatatgat tgtacaacaa caaaatgaaa tggctaatca acagcagcaa
     2461 cagatgtttg agcagcaaca acaaatgttc gatcaacaag ttcagatgag tatgggcttt
     2521 taaaaaggag atgttgatat gcaacatgaa ttagattggt tgagcagata tttagataaa
     2581 aaaggaattg atttgattgt actctatcag ttgagtgtga aagagattat taagtattta
     2641 aggctgagcc cttttcaaag aaaatgtttt aatggaatct attgtagttg tttgtcagat
     2701 tttacatata aagaattgtt gttgattgta aaaagaaatg tatggag
//
LOCUS       DBMDWL010000228         2743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17250_length_2743_cov_2.204769, whole genome shotgun sequence.
ACCESSION   DBMDWL010000228 DBMDWL010000000
VERSION     DBMDWL010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17250_length_2743_cov_2.204769"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="ACZPDN_11215"
     CDS             <1..825
                     /locus_tag="ACZPDN_11215"
                     /inference="COORDINATES: protein motif:HMM:NF017885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR1299221.1"
                     /translation="REKLIKIQQENDMKTDKQIAKQFPYNEMVKLNWDEEAKSIEEKV
                     INLRKFFEIVKLTLLTNNKLTRIACRRLNGTTKADYALIAWAQKAKLEARNHTVKKIN
                     LALLEKEIPKIRSMTIQSPDKFCNKLTQILSNCGIALIFLPHLKGSFLHGATFYDGNK
                     IVIGLTVRGKDADKFWFSLFHEIGHILKGHINQLEGTSQQDEKEADEFASNILIPLDE
                     FKSFISEKNYTKSNIIKFANKSGIDAGILVGRLQKEHYVNFDTFNDLKKKYKIVNI"
     gene            complement(857..2320)
                     /gene="gltX"
                     /locus_tag="ACZPDN_11220"
     CDS             complement(857..2320)
                     /gene="gltX"
                     /locus_tag="ACZPDN_11220"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1299222.1"
                     /translation="MNKTRTRFAPSPTGYMHIGNLRTALFEFLIAKHEGGDFLLRIED
                     TDQERKVEGAVDVIYKTLKECGLNWDEGPDIGGDFGPYVQSERLPMYKGYAEELIKLG
                     GAHYCFCSEEEIEAQRKEAESLGISFKFNDPCKHLSAEEVQAKLDAGEPYVIRQTIKN
                     VGETYFDDEVYGRIEVDGDVLDEQILIKSDGYPTYNFANIIDDHQMQISHVVRGNEYL
                     SSTPKYNLIYDAYGWERPTYVHVPPVMKDEHHKLSKRNGDASFQDLVAKGYLPDAIMN
                     YIALLGWAPETEQEIYTLDELIKVFNIHRISKSPAIFDIDKLTWMNGVYLRNMDEDTY
                     YETVRPYLEKAVHGPYNLKEIAKIIQPRIDILNQIEESVDFFDTLVDYDLEMYRHKKM
                     KTNPEIALKALKASKAALEAFDQWDSMEALHDCLLALPKELEMKNGQVLWPVRTAVSG
                     KQFTPGGAIEIAFILGKDETLRRIQVGIDRLSEIVEE"
     gene            complement(2362..2625)
                     /locus_tag="ACZPDN_11225"
     CDS             complement(2362..2625)
                     /locus_tag="ACZPDN_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR1299223.1"
                     /translation="MDTKAYVYILECKDNTYYTGYTTNVKHRLKMHNLGRGAKYTRAR
                     RPCRLVYSKECDSKSSAMKLEYRIKQLSRKEKLDLILGVLKEF"
BASE COUNT          968 a          529 c          376 g          870 t
ORIGIN      
        1 agggaaaagt tgataaaaat ccaacaggaa aatgatatga aaacagataa acaaatcgca
       61 aaacaatttc catataatga aatggtaaaa ttaaactggg atgaggaagc gaaaagtata
      121 gaagaaaaag taataaattt aaggaaattt tttgaaatag ttaaattaac tcttcttaca
      181 aataataaac taactcgaat tgcttgtaga agattaaatg gaaccacaaa ggccgattat
      241 gctttaatcg catgggcaca aaaagctaaa cttgaagcaa gaaatcatac agtaaaaaaa
      301 atcaatctgg cgcttttaga aaaagaaatt cctaaaatta gaagcatgac gatacaatct
      361 ccggataagt tttgtaataa actaacgcaa atattatcga actgtggtat tgccttgatt
      421 tttttacccc atctaaaagg atcatttcta catggtgcga cattttatga tggtaataaa
      481 attgtcatag gtctaacagt acgtggaaaa gatgcagata aattctggtt tagtctattt
      541 catgaaatag gacatatttt aaaaggtcat atcaatcaac ttgaaggaac ttcacaacaa
      601 gatgaaaaag aagcagatga atttgcatca aacatcctta tcccactaga tgaatttaaa
      661 tcatttattt cagaaaaaaa ctatacaaaa tccaatatta ttaaattcgc aaataaatca
      721 ggaatagatg ctggaatatt agtaggaaga ctacaaaaag aacattatgt aaattttgac
      781 acatttaacg atttaaaaaa gaaatataaa atagtaaata tataaaaagt tcagcaaaat
      841 tgctgaactt tttatgttac tcttctacga tttcacttaa acgatcaata cccacttgaa
      901 tacgacgcaa tgtttcatct ttacctaaga taaacgcaat ttcaatcgct ccacctggag
      961 tgaattgttt accactaaca gctgtacgaa ctggccacaa tacttgtcca ttcttcattt
     1021 ccaattcttt tggaagagcc aataaacaat catgtaaagc ttccatacta tcccattgat
     1081 caaatgcttc taaagcagct ttgctggctt ttaaagcttt taaggcaatc tcaggatttg
     1141 tcttcatctt cttatgacga tacatctcta aatcataatc cactaatgta tcaaagaaat
     1201 caacactctc ttcgatttga ttcaagatat caatacgagg ttgaatgatt tttgcgattt
     1261 cctttaagtt ataaggtcca tgaaccgctt tttctaagta tggacgaact gtttcataat
     1321 aagtatcttc atccatattt cttaaataaa caccattcat ccaagtcaat ttatcaatat
     1381 caaaaatcgc tggagattta gaaatacgat gaatattaaa cactttgatc aattcatcca
     1441 aagtatagat ttcctgttca gtttccggag cccaaccaag taaagcgata tagttcataa
     1501 tcgcatctgg caaatatcct ttcgcaacta aatcttggaa agaagcatcc ccattacgtt
     1561 ttgataattt gtggtgctca tccttcatta caggaggtac atgcacatat gtaggacgct
     1621 cccatccata tgcatcataa atcaagttgt atttaggtgt acttgataaa tattcatttc
     1681 cacgaacaac atgagaaatc tgcatctggt gatcatcaat aatattcgca aagttataag
     1741 ttggatatcc atctgattta attaagattt gttcatctag aacatctcca tccacttcaa
     1801 tacgaccata tacttcatca tcaaaatatg tttcacccac atttttgatt gtttgacgaa
     1861 tgacataagg ttcacccgca tctaatttag cctgtacttc ctcagcgctc aagtgcttac
     1921 aaggatcatt gaacttaaaa gaaataccta agctttcagc ttcttttctt tgagcctcaa
     1981 tctcctcttc actacagaaa caatagtgag ctccacctaa tttgatcaat tcttccgcat
     2041 accctttata cataggtaaa cgttcacttt gaacataagg accaaagtca ccaccaatat
     2101 ctggtccttc atcccaattc aaaccacact cttttaaagt tttataaata acatctacag
     2161 caccttctac tttacgctct tggtctgtat cctcaatacg caataagaag tcgccacctt
     2221 catgtttggc aattaaaaat tcaaataaag ccgtacgcaa atttccaatg tgcatatagc
     2281 cagttggact cggcgcaaaa cgagttcttg ttttattcat agtctatatc atcctttcgc
     2341 tcctattata ataatacgaa actaaaattc tttcaataca cctagaatca agtctagctt
     2401 ttcttttcgc gacaattgtt tgatcctata ctccaatttc attgccgaag atttagaatc
     2461 acactcttta ctatatacca acctgcaagg tcttcttgcc cgcgtatatt ttgcacccct
     2521 acccaaatta tgcatcttca aacgatgttt tacatttgtt gtataccccg tataatacgt
     2581 attatccttg cattctaata tataaacata agcctttgta tccatatgcc tatattaaca
     2641 caaaggggtt gtaacgaata agtaaatttt attacttatg gagttcagct cctctctttt
     2701 tgtttgatta gcttttacat aaataaaaaa tgaaaataca cat
//
LOCUS       DBMDWL010000229         2717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17491_length_2717_cov_3.409021, whole genome shotgun sequence.
ACCESSION   DBMDWL010000229 DBMDWL010000000
VERSION     DBMDWL010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17491_length_2717_cov_3.409021"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            123..2639
                     /locus_tag="ACZPDN_11230"
     CDS             123..2639
                     /locus_tag="ACZPDN_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1299224.1"
                     /translation="MDKEEDASFIANAIKKRFFQGEKLNDLKDFRKKEWCLTIDEIKS
                     SNCVLYKEKLNELQSNNTVPDSLINHIIPNPLLQTGTISANVVEKKVTTNVFLGYIEK
                     ILGKDEIPCKDVVVRLCEHSRNFLVDSGTTDSIIGYSKTDKNGKVTFKGLDPNKSYSV
                     LPISSRYKYGNEKGTFGGNLLSVAKDGNLKINQPFIQRPITIQMFSNSTLNKMKTEHS
                     IIVRSPKSFKKAFVKDIVTVLFCWIALFVTLSIIAIRKGVTFEKSIITCLTFLTCISL
                     IMMYSMTDPLTDGLRGHDTAVGIEVGIVAIALLYFTNIISFYQDKSKISFDSLSEKYS
                     FIPKGFSYVLVALLLTFLLFTPLGKDVGGMKVNLDLGFKFQPSEIAKYLVVVYMAAFF
                     CTHENAIIKYSQTGNAGLFWSKVRYMGAIIIGLSFLILLYMFLGDMGPGLVIAITFII
                     LYSSIKSKIIIQNGKSWNYSQIFSSDIMLLFWGVVSFCVMLYIGSTLEVTWLFALIWF
                     ILWLAGGFLLKKQIYESPVMFNLIITLFVFGGRLFNFLSNIPLLEKLQSIGERLDSRA
                     QMCINTWGELGIGDSVLASENSQVADGLWGLASGGFCGQGIGEGYPDRIPAFHTDMIL
                     SSIGENLGFVFLLAIVIVLSILLKKSVNAGYRTGNKFGLFLAMGIAIVTAVQFFVIAF
                     GSTGIIPLTGVTVPFLSYGKVSLILNLFAFGVILSMSKRVIKQKPSTTLNYKYTISLI
                     SLVYVVLAFGICGTYLNYQLFNRNSTLVRPLFVTNNSGVPLIEYNPRIQILTSLLHAG
                     NIYDRNGVLLATSNVDTLRSHADDYETCNIDVKDILGVVV"
BASE COUNT          845 a          397 c          530 g          945 t
ORIGIN      
        1 taaaaggagt catagacata ccattcttca tgtatcgttt agcaacagtt ttgggttagc
       61 atctgcacgg cctactccac agggtttgtc gggtgcgcca atattttatt aagaaaggtt
      121 acttggataa agaagaagat gcgtcattca ttgccaatgc catcaaaaaa agattttttc
      181 aaggagaaaa gttaaatgac ctaaaagatt tcaggaaaaa agaatggtgt ttgactatag
      241 atgagataaa gtcctcaaat tgtgttttat acaaagaaaa attaaacgaa ttacaatcaa
      301 acaatacagt cccagactct ttaattaatc atataatacc taatcctttg ctacaaacag
      361 gaactataag tgccaatgta gtggagaaaa aggtaactac aaatgtattt ttaggttata
      421 tagagaaaat attaggaaaa gatgaaattc catgtaaaga tgttgttgtg agattatgtg
      481 aacatagtcg taatttcctt gttgattcag ggacaaccga tagtatcata ggttattcca
      541 aaacagacaa aaatggcaag gtcactttta aagggctaga tccaaataaa agttatagcg
      601 tattacctat atccagtcgg tataaatatg gtaatgagaa gggcactttt ggtgggaatc
      661 ttctttctgt ggcaaaagat ggaaatctca aaattaatca gcctttcatt caacgtccta
      721 ttacaataca aatgttttca aattctacgt tgaataagat gaagactgaa cactctataa
      781 tagttcgttc tccaaaaagt ttcaaaaaag catttgtaaa agatattgtt actgtattgt
      841 tttgttggat agcattattc gtaactttat ctataatagc tataaggaaa ggtgtaacgt
      901 ttgaaaaaag cattataacc tgtctaacat ttttgacatg tattagtcta ataatgatgt
      961 actcaatgac cgaccctctt acagatggat tacgaggtca tgacactgct gtagggattg
     1021 aagtaggaat agtggctatc gctttactgt attttacaaa tataatttct ttctatcagg
     1081 ataaatcaaa aattagtttc gatagccttt cagaaaaata ttcgtttatt cccaaaggat
     1141 tttcttatgt gttagttgca ttgcttttaa cgtttctttt atttacacca ttaggaaagg
     1201 atgttggagg aatgaaagta aatcttgatt taggcttcaa atttcaaccg agtgagatag
     1261 ccaagtattt agttgtggta tatatggctg ctttcttctg tacccatgaa aatgcaatta
     1321 ttaaatactc ccaaactgga aatgcaggat tattttggag caaagttagg tatatgggag
     1381 ccataatcat cggtctaagt ttcttgatat tattgtacat gtttttaggg gacatgggac
     1441 caggactagt tattgctata acatttataa ttttgtattc gtcaataaag tccaagataa
     1501 ttatacagaa tggaaaatca tggaattatt cgcagatatt ctctagtgac atcatgttac
     1561 tattttgggg agttgttagt ttctgcgtta tgctgtatat cggttccaca ttggaagtta
     1621 cttggctatt tgctctcatt tggttcattt tgtggcttgc gggaggtttc ttattaaaaa
     1681 aacaaattta cgaatctcct gtaatgttta atctgataat aactttattt gttttcggtg
     1741 gtcgtttgtt taatttctta tcaaatattc ctcttttgga gaaattacaa tcaataggcg
     1801 aacgtcttga tagcagagct caaatgtgta ttaacacatg gggtgaattg ggaattggag
     1861 attctgtgtt ggcaagtgaa aatagtcaag tggcagatgg actttggggg cttgcgtctg
     1921 gtggtttctg tggacaagga attggtgaag gttatcctga tagaattcca gcttttcata
     1981 ctgatatgat attgtccagt atcggcgaga atttgggctt tgtattcttg cttgcaattg
     2041 ttattgtttt atcaatttta ctgaaaaaat cagtcaatgc tggctataga acaggaaaca
     2101 agttcggcct tttccttgct atgggtatcg cgattgttac agctgttcaa ttttttgtca
     2161 tagcttttgg tagtacagga atcattcctt taacaggtgt taccgttcca tttcttagct
     2221 atggaaaagt aagccttatt ttaaacttat ttgcctttgg ggtaattctt tcaatgtcga
     2281 aacgagtaat aaagcagaag ccatctacta ctctaaacta caaatatacc atatcattaa
     2341 taagtcttgt ttatgtagtg ttagcatttg gtatttgtgg aacttactta aattatcagc
     2401 ttttcaatcg taacagtacg ttggtaaggc ctttgtttgt tactaacaac agtggcgttc
     2461 ctctaataga gtataatcct cgtattcaaa ttttgacaag tttgttacat gctggtaata
     2521 tttatgatag aaatggagtg ctactcgcta caagtaatgt ggacacttta cgttctcatg
     2581 ctgatgacta tgaaacttgt aatatagatg ttaaggatat attaggggtt gtcgtttaat
     2641 tctaagaatt caatatctat agacgagggt atgccttccg aggttgccat cgtctgctta
     2701 ttttcggagt tttatcg
//
LOCUS       DBMDWL010000230         2708 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17590_length_2708_cov_1.647871, whole genome shotgun sequence.
ACCESSION   DBMDWL010000230 DBMDWL010000000
VERSION     DBMDWL010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17590_length_2708_cov_1.647871"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="ACZPDN_11235"
     CDS             complement(<1..469)
                     /locus_tag="ACZPDN_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012451.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR1299225.1"
                     /translation="MNAWEILGIEPTSDKKTIKRAYAKLLKQYHPEENPEKFKQIQAA
                     YQQCLHSDQEIESVSYEQNIESKQDIKTKPISIKEDTIVPPPIPKITTLFIQNEKDVV
                     VYKDILNSIEKSLPKKIGSKDIVQVFTDSKIMPYLEDELFCRRLEDIFLSRKLK"
     gene            complement(466..2205)
                     /locus_tag="ACZPDN_11240"
     CDS             complement(466..2205)
                     /locus_tag="ACZPDN_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR1299226.1"
                     /translation="MTILGVDLGTTNSLAVVYKEGKPVRIPNAYGEFVTPSAVSILDG
                     KIVVGKLAKERLITHPECSASLFKRNMGSDVTYTLDRKEYDSATLSSFVVKQLIEDAQ
                     NYLHEEISEVVISVPAYFNARQRQDTKRIGELLGIKVERLINEPSAAAIACHMDDEYE
                     TFVVFDFGGGTLDVSVVDCFENVISINAISGNNHLGGTDFNRAMAEYFCLKNELNYNV
                     LDSSFQQSILRTCEQAKIKLSTQNVVEVSLVHLNKNYNCIFDENVLFNITHSLLESCK
                     NVIGKAVKDSGFSASELDSLILVGGSSKMPVLQHYLSDALNIPVLKEEHMDSLVVLGL
                     GKYIGIKQRDENIKDVVVTDICPFSLSTSTYNEQNPDLELSTVLIPKNSVLPTSKKMT
                     LRTVHKGQTKVNISVFQGQAMYAKENLFLGQAFIHVPRNMHDYESFDLIYSYDINSML
                     YVEAVVHSTKEHYIFRVSKGDVLEKVDASVRLDSIKEVSLGLYQNNEVDALLARIERI
                     YREVDEETQDYLMKLHTEFTKDMESLINNIQKRKRLINQVSRILDQIEESQNVDQLDI
                     FTQEEDEEGEYLA"
     gene            complement(2215..2496)
                     /locus_tag="ACZPDN_11245"
     CDS             complement(2215..2496)
                     /locus_tag="ACZPDN_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299227.1"
                     /translation="MGGLCVLLLMMFCVGWILCVFFLSMLIPLLIIYNGIVGIVCIIL
                     YFVLRKKSVFIKYAEGYKHVLSIVLKYFLIVYGSISLVFSIILAILNFC"
BASE COUNT          899 a          520 c          361 g          928 t
ORIGIN      
        1 attttaattt acgagataaa aagatgtctt ctaatcttct acaaaacaat tcatcctcta
       61 aatacggcat gattttagaa tctgtaaaaa cttgtacaat atctttagaa ccaatctttt
      121 tgggcaaact tttttcaatc gaatttaata tatctttata aacaacgaca tccttttcat
      181 tctgaataaa taatgtcgtt attttaggaa tgggtggagg aacaattgta tcttctttaa
      241 ttgaaatggg ttttgtttta atatcttgtt tactttctat attttgttcg tatgaaacag
      301 attctatttc ttgatctgaa tgtagacact gttgataagc cgcttgaatc tgtttaaatt
      361 tctctggatt ttcttcagga tgatactgct ttaaaagctt ggcatacgcc cttttaattg
      421 tctttttatc acttgtaggc tcaatcccta atatttccca cgcattcatg ccaaatactc
      481 tccttcttca tcctcttctt gagtaaagat atctaattga tctacatttt gactttcttc
      541 tatctgatct aaaattcgtg aaacctgatt aataagacgt tttctttttt gaatattgtt
      601 gatcaaactt tccatatctt ttgtgaattc agtatgaagt ttcatcaaat aatcctgtgt
      661 ttcttcatcc acttcccgat aaattctttc aatacgagca agcagggcat ccacttcatt
      721 gttttgatat aaacctaaag atacttcttt gatagaatct aaacgaacag acgcatctac
      781 tttttctagc acatcgcctt ttgatactct aaaaatataa tgttccttag tagaatgtac
      841 aacggcttct acatacaaca tggaattaat gtcatacgaa tagatcaaat caaaagattc
      901 ataatcatgc atatttctag gaacatgaat aaaagcttgg cctaaaaata agttttcctt
      961 ggcatacata gcctgccctt gaaacacact aatattgact ttagtctgcc ctttatgcac
     1021 ggtacgcaaa gtcatctttt tacttgttgg taaaactgaa ttcttaggaa taagcacggt
     1081 tgataattct aaatctggat tttgttcgtt atacgtagaa gtacttaagg agaaaggaca
     1141 aatatcagta acaactacat ctttaatatt ttcatccctt tgtttaatgc caatgtattt
     1201 tccaagtcca agtacaacta aagaatccat gtgttcttct tttagtacag gaatatttaa
     1261 agcgtctgat aaataatgtt gaagtaccgg cattttactt gagcctccta caagaatcaa
     1321 actatcgagt tcactggctg aaaaaccgct gtctttcaca gctttaccaa tgacattttt
     1381 acaagattct agtaaggaat gtgtaatatt gaataataca ttttcatcaa atatacaatt
     1441 ataattctta tttaaatgaa ctaaagaaac ttcaactaca ttttgcgtac ttaacttgat
     1501 tttagcttgt tcgcaagtac gcaaaatgct ttgttgaaaa gaagagtcta agacattata
     1561 gtttaactca ttttttaaac aaaagtattc ggccatagct cgattaaaat ctgtgccacc
     1621 caagtgatta tttccgctaa tcgcattgat actaatcaca ttttcaaaac agtcaacaac
     1681 tgaaacatct aatgttcctc caccaaaatc aaatacaacg aaggtttcat attcatcgtc
     1741 catatgacat gcgatagctg cagcacttgg ttcattgatc aaacgctcca ctttaattcc
     1801 taaaagttcg cctattcgtt ttgtatcttg tctttgacgt gcattaaaat aagcaggcac
     1861 actaatcaca acttctgata tttcttcatg aagataattt tgcgcgtctt caatcaattg
     1921 tttcacgaca aaagaagaaa gtgtagctga atcatactct ttcctatcta atgtgtaagt
     1981 aacatctgaa cccatatttc gcttaaacaa agatgcacta cactctggat gcgtaattaa
     2041 tctttcctta gccaattttc caacaacaat cttaccatcc aagatactga cagcacttgg
     2101 agttacaaat tcgccatacg cattgggaat ccgtactggc tttccttctt tatagacaac
     2161 cgctaaactg ttggttgttc ctaaatccac acctaatatt gtcatatttt tctcctaaca
     2221 gaagtttaaa atagctaata tgatactaaa tactaatgaa atacttccgt atacaattag
     2281 aaaatatttt aatacaatgg atagtacatg tttatagcct tctgcatatt taataaaaac
     2341 agattttttg cgtagtacaa aatatagaat gatacataca atgcctacaa ttccattata
     2401 gatgatcaaa agtggaatca acatcgataa aaagaacaca catagtatcc atcctacaca
     2461 aaacatcatt agtagtaata cacacaatcc gcccataata tcgccttctt taactccact
     2521 atagcatact aaaagcggta ttactaccgc ctagaagtct ttgtggaatc ttcctccata
     2581 atgattgtct ttatagtttg tgatatacaa atataagact aatacaccta caagaataat
     2641 gcctacaata aataaagtat gagctgacat ctttccatat aggaacatgc atccacaccc
     2701 aacaagta
//
LOCUS       DBMDWL010000231         2701 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17645_length_2701_cov_2.789231, whole genome shotgun sequence.
ACCESSION   DBMDWL010000231 DBMDWL010000000
VERSION     DBMDWL010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17645_length_2701_cov_2.789231"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="ACZPDN_11250"
     CDS             complement(<1..963)
                     /locus_tag="ACZPDN_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1299228.1"
                     /translation="MMMEQIANRYEILSLIGQGGMADVYKAKDTILNRIVAIKVLRDK
                     LSQDAMALVRFQREASAASRLSHPNVVDIYDVGEYEGMHYIVMEFIRGRTLKELIAQR
                     GALDVDEAIGIMKQLVSAINHAHEHKIIHRDIKPQNVLVKDDGTIKITDFGIAVANGS
                     VQLTFNNTVMGSAHYLAPETTQGKEPNEQVDIYSLGIVFFELLTGKVPFTGKTPTEIA
                     IKHLRKPMPYVRDFNPNIPQSVENIILKATAKNLSDRYVSCKEMLYDLIHCMDDEYRN
                     MKRIKVDQDQTSELLKFDNGHLDFEDEEPAKVEKKQRNWIPALMI"
     gene            complement(965..1705)
                     /locus_tag="ACZPDN_11255"
     CDS             complement(965..1705)
                     /locus_tag="ACZPDN_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR1299229.1"
                     /translation="MKVFANTNVGLVRKLNEDDYCIAHNDNDEWMAIVCDGIGGAKAG
                     EVASHTAVMTMYEAFMKAPKFNKDRQVDNWIRENLNQANDFIYSKSLKNPNERGMGTT
                     AVGVIVASIGTFIFNVGDSRIYADYNGELIQMSEDHSVIAQLLKEGKITSEEAKTHPK
                     KNTLTNALGVWHVFRIDINKIENTYNYLLISSDGLHGYVEKKVISDIVHDESLSLQEK
                     VDTLIARANQSGGYDNCSIILMENDGEQ"
     gene            complement(1710..>2701)
                     /gene="rlmN"
                     /locus_tag="ACZPDN_11260"
     CDS             complement(1710..>2701)
                     /gene="rlmN"
                     /locus_tag="ACZPDN_11260"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1299230.1"
                     /translation="HGWKSYRGHQIFQWLYRNRVFDIDEMTNVSKETREILKKDFIVN
                     PLELIKKQVSHDGTTKFLFKTSDGALLESVMMVFDYGRSVCVSSQVGCNMGCAFCASG
                     LTKKKRDLTSGEMVAQVMYVQKELDKDNLRLSHIVVMGTGEPFDNYDNVMNFLATVNH
                     DRGLGIGSRHITISTCGIVPRILDFANEHTQYNLAISLHAPNDELRDQLMPVNHAYPL
                     KELMEAIQYYGKENNRRLTFEYILLKGVNDHPEHAKQLSKLLHGMNAYVNLIPYNAVD
                     EKGFKSVTHDEAMVFYDLLMKNHVRCTIRKEHGNDIDAACGQLRIKEIKKEGI"
BASE COUNT          803 a          592 c          366 g          940 t
ORIGIN      
        1 aatcattaaa gcaggaatcc aatttctttg tttcttttcc acctttgcag gctcttcatc
       61 ctcaaaatca agatgaccat tatcaaattt caacaattca cttgtttgat cttgatccac
      121 tttgatacgt ttcatattcc ggtattcatc atccatgcaa tgaatcaaat cataaagcat
      181 ttctttacaa gaaacataac gatcggatag atttttagcc gtcgccttta aaataatatt
      241 ctctaccgat tgcggaatat tcgggttaaa gtcacgaaca tatggcattg gcttacgcaa
      301 atgcttaatt gcgatttctg ttggtgtttt tcctgtaaaa ggaactttgc ctgttaataa
      361 ttcaaaaaat acaattccca aagaatatat atcaacttgt tcgttgggct cttttccctg
      421 tgttgtttca ggagctaagt agtgtgctga acccatgact gtattgttaa aagttaattg
      481 tacagatcca ttggctaccg caatcccaaa atcagtgatt tttattgttc catcatcttt
      541 aaccaaaaca ttttgtggtt ttatgtcacg atgaataatt ttatgttcat gcgcatgatt
      601 tatcgcactc accaattgtt tcataatgcc tatggcctca tcgacatcta aggcaccacg
      661 ctgcgcaatc aattctttca atgttcttcc acgaataaat tccataacaa tatagtgcat
      721 accttcgtat tcaccaacat catatatatc cactacattg gggtgggata gtcgacttgc
      781 tgcacttgcc tcacgttgaa aacgaacaag tgccatcgca tcttgactta atttatcacg
      841 tagaactttg attgcgacaa tacgatttaa aattgtatct tttgccttat agacatccgc
      901 cataccacct tgtccaatca aagacaagat ttcataacga ttcgctatct gttccatcat
      961 catattattg ttctccatca ttctccatta agataattga acaattgtca taaccacccg
     1021 attggttggc tcttgcgatc aatgtatcca ctttttcctg taaagaaaga ctttcatcat
     1081 gcacaatatc tgaaattact ttcttttcca catatccatg aagcccatca cttgagatca
     1141 ataaatagtt atatgtattt tcaatcttat tgatatcaat acggaacaca tgccatacac
     1201 caagtgcatt tgtcaatgta ttttttttag gatgtgtttt tgcctcttca cttgtgattt
     1261 tgccttcctt taaaagttgg gcaattacac tatgatcttc actcatttga atcaattccc
     1321 cattataatc ggcatagatt cttgaatctc ctacattgaa aataaaggtt ccaatgctag
     1381 ctacaataac acccacagcg gttgttccca tacctctttc gttcgggttt tttaaacttt
     1441 ttgaataaat aaaatcattc gcctgattta aattttcacg aatccaattg tccacctgtc
     1501 gatctttatt aaatttagga gccttcataa atgcctcata cattgtcatg accgctgtat
     1561 gacttgccac ttctccggct tttgccccgc caataccatc acagacgatg gccatccatt
     1621 catcattgtc attgtgggca atgcaataat catcttcatt taattttctt acaagaccta
     1681 cattcgtatt cgcaaaaact ttcatcgact taaattcctt ccttttttat ttccttaatt
     1741 cttaactgac cacaagctgc atcaatatca ttaccatgct cttttcgaat tgtgcaacgc
     1801 acatgatttt tcatcaacaa atcatagaat accatcgcct catcatgtgt aaccgatttg
     1861 aagccttttt catccaccgc attatatgga atcaaattta cataggcatt cattccatgt
     1921 aaaagcttcg ataattgttt ggcatgttca ggatgatcat tgacaccctt taataaaata
     1981 tattcaaatg tcaaacgacg attattttcc ttaccataat attgaattgc ttccatcaat
     2041 tcctttaatg gatatgcatg gtttacaggc atcaattgat cacgcaattc atcatttggt
     2101 gcatgtaaac tgatggccaa attgtattgg gtatgctcat tggcaaaatc caagattcta
     2161 ggcacaattc cacaagtcga aattgtgata tgtcttgaac caatacctaa accacgatca
     2221 tgattcacgg tagctaaaaa attcataaca ttgtcatagt tatcaaatgg ctctcctgtg
     2281 cccatgacaa caatgtgtga taggcgcaaa ttatctttat ccaattcttt ttgaacatac
     2341 ataacttggg caaccatttc tccacttgtc aaatctcttt ttttctttgt aagcccactt
     2401 gcacaaaatg cacaacccat attacatcca acttgacttg aaacacatac acttcttcca
     2461 tagtcaaaga ccatcataac actttctaat agggctccat cacttgtctt aaacaagaat
     2521 tttgtcgttc catcatgtga aacttgtttt tttattaatt ctaaaggatt cacaataaaa
     2581 tctttcttca agatttctct tgtttccttt gatacatttg tcatttcatc aatatcaaat
     2641 acgcggttac gatataacca ttgaaaaatt tgatgtccac gataagattt ccatccatga
     2701 t
//
LOCUS       DBMDWL010000232         2673 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17901_length_2673_cov_3.259720, whole genome shotgun sequence.
ACCESSION   DBMDWL010000232 DBMDWL010000000
VERSION     DBMDWL010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17901_length_2673_cov_3.259720"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="ACZPDN_11265"
     CDS             <1..167
                     /locus_tag="ACZPDN_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR1299231.1"
                     /translation="TVINNNPVFKKYYLLKISQGKGHRCAQGHCIRKLLRIIYHLLET
                     GQSFDPALLR"
     gene            complement(371..796)
                     /gene="ruvX"
                     /locus_tag="ACZPDN_11270"
     CDS             complement(371..796)
                     /gene="ruvX"
                     /locus_tag="ACZPDN_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1299232.1"
                     /translation="MDRIIGLDLGSKTCGVSICDALHITCRALTTVRFESDDYDACLE
                     EVLKLLQENKVKEVVLGLPKHMNGDIGIRGEISCEFAKKLESSGIKVHLWDERLTTVA
                     AEKILLQGDVSRKKRKKVIDQMAAVQILQSFLDRQNGGF"
     gene            complement(796..1047)
                     /locus_tag="ACZPDN_11275"
     CDS             complement(796..1047)
                     /locus_tag="ACZPDN_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR1299233.1"
                     /translation="MKDITETMTFTTEEIRRENIKMILREVSDALEERGYNSENQIAG
                     YLISNDPAYISSHKNARNLIQKIERYEIIEELVRAYLDK"
     gene            complement(1126..1377)
                     /locus_tag="ACZPDN_11280"
     CDS             complement(1126..1377)
                     /locus_tag="ACZPDN_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299234.1"
                     /translation="MRKIIVDLNRVKDDEYVAMYEIFGLDVLNKSYEDFERRMLQIQI
                     ETIVEVKNRKHNLSTCSKWIFILEDIQQKSDYFYCIWGV"
     gene            complement(1380..>2673)
                     /locus_tag="ACZPDN_11285"
     CDS             complement(1380..>2673)
                     /locus_tag="ACZPDN_11285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1299235.1"
                     /translation="IGFLVMMLSISWKMTLMALIVIPLSLILVMFVVKKSQRYFKAQQ
                     KSLGLVNGHIEEMYSAHTIVKAFNGEEDSLRTFKEYNDQLYTSAWKSQFLSGLMMPLT
                     NLIGNISYVGVCILGGYLTIHKEIAVGDIQSFITYTRNFTQPISQISQSMNMLQSTAA
                     AAERVFEFLAAEEEICDVKNPVVLKDVQGQVTFENVCFGYDPNKTVINNFSMYIKPGQ
                     TTAIVGPTGAGKTTIVKLLMRFYELNAGSIYIDGHNITEYTRSGLRNMVGMVLQDAWL
                     FEGSILENLRFGKPSATDDEVIQAAKAAHVDHFIHTLDHGYDTVLNESSSNISQGQRQ
                     LLTIARAFLADPKILILDEATSSVDTRTEVLIQKGMDELMKGRTSFVIAHRLSTIRDA
                     DNIIVMDHGDIVEVGNHDELMKRNGFYTKLYNAQFEEA"
BASE COUNT          899 a          553 c          346 g          875 t
ORIGIN      
        1 tgactgtaat taacaataat cctgtattca agaaatacta tctattaaag atttctcaag
       61 gtaaaggaca ccgttgtgct caaggccact gcattagaaa gctgcttaga atcatttatc
      121 atttactaga aacaggacaa tcatttgacc ctgcattatt aagataagac aaatgcatct
      181 aaagcatctg aacttttaaa aaatcgaatt ttaaaagttc ttttcgtcat gcttaaattt
      241 aatccaatat tcacattttc aaagatcaat tcaaaactaa ctaaaaaatc tagctatttg
      301 atattttcaa actttttcat aaataatact tgaattttat atagttagct ttcaaaaata
      361 aaggaggaca ttaaaatccc ccattttgtc tgtccaaaaa actctgtaaa atttgaaccg
      421 cagccatttg atcaattact tttttacgtt tctttctaga aacatccccc tgaagtaaaa
      481 ttttctcagc tgcaactgtt gtaagtcgct catcccatag atgaacttta attccagaag
      541 attctaattt cttggcaaat tcacaagata tttcaccacg aattccgata tctccattca
      601 tgtgcttcgg taagcctaaa actacttctt ttactttatt ttcctgaagc aatttcaaaa
      661 cttcttctaa acaagcatca taatcatccg attcaaaacg cacagtcgtt aaagcacgac
      721 acgtaatatg aagcgcatca caaatcgata caccacacgt tttcgaacca agatctaaac
      781 caatgatacg atccattatt tatccaaata tgcacgtact aattcttcaa tgatttcata
      841 acgttcgatc ttttggatca agttacgagc attcttgtga gaagaaatat acgcaggatc
      901 attactgatc aagtagccag caatttgatt ttctgaatta taaccacgtt cttccaaagc
      961 atcacttact tcacgaagaa tcatcttgat attctctcga cgaatctctt ctgtagtaaa
     1021 tgtcattgtt tcggtaatgt ctttcataat ttcacactcc ttaacacatt tttattatat
     1081 atgaatccta accaaaaaga aagcgattta gtagaaaatg ggatgttaaa caccccatat
     1141 acagtaaaag taatcacttt tttgttgaat atcctctaag ataaagatcc atttcgaaca
     1201 agtagaaagg ttatgtttcc tattttttac ttcgacaatc gtttcaattt gaatttgaag
     1261 cattcttctt tcaaaatcct catatgactt attaagtaca tccaatccaa agatttcata
     1321 catcgctaca tattcatcat ctttgactcg atttaaatcc acaatgatct tacgcatcac
     1381 taagcctctt caaattgcgc attgtataac tttgtataga aaccattacg cttcatcaat
     1441 tcatcatgat ttccaacttc tacaatgtca ccatgatcca tcacaataat attatccgca
     1501 tcacgaatcg tggataaacg gtgcgcaatc acaaaacttg tacgtccctt cattaactca
     1561 tccatacctt tttgaatcaa gacttccgta cgtgtatcca cactacttgt tgcttcatcc
     1621 aaaattaaga ttttaggatc ggctaaaaaa gcacgggcaa tcgttaataa ttgtctttga
     1681 ccttgactaa tattacttga agattcattt aatacagtat catatccatg atctaatgta
     1741 tgaataaagt gatcgacatg agctgcctta gctgcttgga ttacttcatc atctgtagcg
     1801 cttggcttac caaaacgtaa gttttctaaa atactaccct caaatagcca ggcatcctgt
     1861 aagaccatac caaccatatt acgcaaacca cttcttgtat attctgtaat attatgacca
     1921 tcaatataaa tggatcctgc attcaattca tagaaacgca tcaataattt gacaatcgtt
     1981 gttttaccag cgcctgtagg accaacaatt gcggtcgttt gtcctggctt aatatacatc
     2041 gaaaaattgt tgatgacagt tttatttgga tcatatccaa aacatacatt ttcaaaagtc
     2101 acttgacctt gtacatcttt taatacaact ggatttttta catcacaaat ttcttcttct
     2161 gccgccaaga attcaaaaac acgctctgca gctgcagctg tactttgaag catattcatt
     2221 gattgtgaga tttgagagat cggttgcgta aagttacgtg tatatgtaat aaacgattga
     2281 atatctccca cggcaatttc tttatgaatc gttaaatatc cacctagaat acatacaccg
     2341 acatagctga tatttccaat taagttcgtt aatggcatca taagtccaga taagaactgt
     2401 gatttccaag cactcgtata caattgatca ttatattctt taaaagtacg caaagaatct
     2461 tcttccccat taaatgcctt cacaattgta tgtgccgaat acatctcttc aatatgacca
     2521 ttcactaaac ccaatgattt ttgttgagcc ttaaaataac gctgactctt tttgacaacg
     2581 aacataacta agatcaaact caatggaatg acaattaaag ccataagtgt catcttccaa
     2641 gaaatactaa gcatcatgac taaaaagcca ata
//
LOCUS       DBMDWL010000233         2671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17920_length_2671_cov_2.071984, whole genome shotgun sequence.
ACCESSION   DBMDWL010000233 DBMDWL010000000
VERSION     DBMDWL010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17920_length_2671_cov_2.071984"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>392)
                     /locus_tag="ACZPDN_11290"
     CDS             complement(<1..>392)
                     /locus_tag="ACZPDN_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299236.1"
                     /translation="KTTLEKFFCSWIIGKHPKDYSLFFSHSNEITGKFYKGVLDITTD
                     DKEYKWNVIFPNLPLQSTNAQAQEANFGKYKAFSSIQCSSIGAKNAGKVRTNRYLYCD
                     DLIGSIEEALNPIILEKIWRIYGVDLKQ"
     assembly_gap    393..402
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<403..853)
                     /locus_tag="ACZPDN_11295"
     CDS             complement(<403..853)
                     /locus_tag="ACZPDN_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299237.1"
                     /translation="MNSELNQLIDDCEKYISQNGIDENIIETYYNVCQLAKNEREIDA
                     MLKCTARAKELIEKACMRDIGIDIFELEKYTFNNNIDNDLVNRYFDTLLLEAPHLFHS
                     YLLYLEKDREESERFYQPKMKQLNKYGLIQAMQDLEDDKYNRLCISMP"
     gene            complement(954..1175)
                     /locus_tag="ACZPDN_11300"
     CDS             complement(954..1175)
                     /locus_tag="ACZPDN_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299238.1"
                     /translation="MNLAEAKEKYYPKYKYALVSVKNNKPHSLYVDRKTAERYGIKAY
                     TIPAIYLIDNYNALLNKLHPVRNGAMPAF"
     gene            complement(1191..1460)
                     /locus_tag="ACZPDN_11305"
     CDS             complement(1191..1460)
                     /locus_tag="ACZPDN_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299239.1"
                     /translation="MAKPLSKLAEKCKSCPKSEKCDHKRMELCTLMDLPPQNLASTTQ
                     GVLMDNMSPILREEIKSPLSPFRYKDELEKALNDSHFGNMFMNGA"
     gene            complement(1638..1826)
                     /locus_tag="ACZPDN_11310"
     CDS             complement(1638..1826)
                     /locus_tag="ACZPDN_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299240.1"
                     /translation="MAMGVHPLNKDKFYEAINLYISGQASQVKAAKVAGCSVPTFKKY
                     ANKIYGGEELPDNLWGKK"
     gene            complement(1840..>2671)
                     /locus_tag="ACZPDN_11315"
     CDS             complement(1840..>2671)
                     /locus_tag="ACZPDN_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299241.1"
                     /translation="GKYWVYNSYSDWRINNFPYWSEKTIQRTFTRLESKGIVISANYN
                     KLAIDKTKWYTINTKKLQELVDKFNSNEDRMTNRQDNMTDRQDRMTCREGQNDRPLPE
                     ITTENINRDYNPEITNKDNTSINIDGEVHTSFSEKPTARAVARDEMLLKEKDMVDRFN
                     NICDDDIDNSAICDCVKDGFKMYMQLYEIYFHKVHPILTDKTLKNVCFVLSTITDTEH
                     GHFDADTIYETDDNGFTVLQRMINDHFIREHRKSTNYSITHFANAEYLGKLANRFIEM
                     "
BASE COUNT          731 a          490 c          400 g         1040 t
ORIGIN      
        1 ttgctttaaa tcgactccat aaattctcca tattttttca agaattattg gattaagtgc
       61 ttcttcaata gaacctataa ggtcatcaca atataaataa cggttagttc taaccttacc
      121 agcattctta gctcctattg atgagcattg aatacttgaa aatgctttgt atttaccgaa
      181 attagcttct tgtgcctgtg catttgtgct ttgtaatggt aaattaggga aaataacatt
      241 ccatttatat tctttatcat ctgttgttat gtcaagcact cctttataaa actttcctgt
      301 aatttcgttg ctgtgagaaa agaaaaggct gtaatcttta gggtgcttgc caattatcca
      361 agagcaaaaa aatttttcta gtgtagtttt ttnnnnnnnn nngtggcata gaaatacata
      421 atctattata tttgtcgtct tccaaatctt gcatagcttg aataagcccg tatttattaa
      481 gctgtttcat ttttggctga taaaatcttt cactctcttc tctgtctttt tcaagataaa
      541 gcaaatagct gtggaataag tgcggagctt caagcaataa ggtatcaaaa tatctattaa
      601 ctaaatcatt gtctatattg ttgttaaatg tatatttttc aagttcaaaa atatctatgc
      661 ctatatcacg catacaagcc ttttctataa gttcttttgc cctagctgta cattttaaca
      721 ttgcgtcaat ttcacgctca ttcttggcaa gttggcacac gttgtagtag gtttctatga
      781 tgttttcatc tattccattt tgggatatgt atttttcgca atcatctatc agttgattta
      841 gttcagaatt caagaaaagc acctccactt ttcagcaaag gtgcttatag acctctgcct
      901 ataattgttc tagggtagcg actaactcta tttgttagcc ggtaaaattt ttattagaat
      961 gctggcattg cgccattgcg aaccggatgt aatttattca gaagtgcatt ataattatca
     1021 attaaatata ttgccggaat cgtatatgct ttaatgccat atctttctgc tgtttttcta
     1081 tcaacataaa gtgaatgtgg tttgttattt tttacactaa caagtgcgta tttgtatttt
     1141 ggataatatt tttcctttgc ttctgcaaga ttcaccactt caccagcttt ctaagcacca
     1201 ttcataaaca tatttccaaa atgggaatca tttagtgctt tttctaattc gtctttgtac
     1261 ctaaatggac ttaaaggact ttttatttct tccctcaata taggcgacat attgtccatc
     1321 aaaacgcctt gtgtagtact tgcaagattt tgtggtggca aatccattaa agtgcataac
     1381 tccattcttt tatggtcgca tttttcagat ttagggcaac ttttacattt ttctgctaat
     1441 ttacttaaag gtttcgccat tactacacca actttctacc gcagataggg caataattga
     1501 ttttataatc aaaatccata aaactatcgc ctgttgtaaa gtgaataaat acgtcatctt
     1561 ctctcttgta tatataatct ttgtaatcgg ggtttttata atctgatgta taaatgtttt
     1621 cacaaaactt acacatatca cttcttcccc cataaattat ctggtaattc ttcgccgcca
     1681 taaatcttgt tagcgtattt cttaaatgtc ggtacgctac aacctgctac ttttgccgcc
     1741 tttacttgtg aagcctgccc cgatatgtac aggttaattg cttcataaaa cttatctttg
     1801 tttagtggat gtacacccat agccataata atcactcctt tacatttcta taaatctatt
     1861 tgccagcttg ccaagatatt cagcgttggc aaaatgtgtt attgagtagt ttgtgctttt
     1921 tctatgttct ctgataaaat ggtcgttaat cattctctgt aaaactgtaa atccgttatc
     1981 gtctgtttcg tatatagtgt cagcgtcgaa atgtccgtgt tccgtatctg tgatagttga
     2041 taggacaaaa catacattct ttaatgtctt atctgtaagt attgggtgta ctttatggaa
     2101 atagatttca tataactgca tatacatctt aaatccatct ttaacacaat cgcatatagc
     2161 tgaattatct atatcgtcgt cacagatgtt attaaaccta tcaaccatat ctttttcttt
     2221 aagcaacatt tcatctcttg cgactgctct tgccgtcggt ttctctgaaa acgatgtatg
     2281 tacctctcca tcaatgttaa ttgatgtatt gtccttatta gtaatttctg gattataatc
     2341 tctgtttata ttctctgtag taatctctgg taatggtctg tcgttttgtc cttctcgaca
     2401 ggtcattctg tcctgtcggt ctgtcatatt gtcttgtcga tttgtcattc tgtcctcatt
     2461 ggaattaaat ttatccacaa gttcctgtag tttcttagta tttattgtgt accactttgt
     2521 tttatcaata gctaatttat tgtaattagc tgatataaca attcctttac tttcaagcct
     2581 tgtgaatgtt ctctgtatcg ttttttcact ccaatatgga aaattattaa ttctccaatc
     2641 gctgtatgag ttgtaaaccc aatatttacc a
//
LOCUS       DBMDWL010000234         2664 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_17989_length_2664_cov_1.835349, whole genome shotgun sequence.
ACCESSION   DBMDWL010000234 DBMDWL010000000
VERSION     DBMDWL010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17989_length_2664_cov_1.835349"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="ACZPDN_11320"
     CDS             complement(<1..490)
                     /locus_tag="ACZPDN_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1299242.1"
                     /translation="MIIVMKSTASKEDVEKVSGSVEKLGLRVNVVNGATQSVIGIIGD
                     TTKVDPESIEVDPAVEKVMHVSEPYKLANRAFHPEDSVIDVGGVKIGGGHLAVIAGPC
                     SVESKEQVIEIAKAAKAAGANLLRGGAFKPRTSPYAFQGMGSAGLDILVAAKEATGLP
                     IVS"
     misc_binding    complement(546..800)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     assembly_gap    672..681
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1054..2328
                     /locus_tag="ACZPDN_11325"
     CDS             1054..2328
                     /locus_tag="ACZPDN_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_200765090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1299243.1"
                     /translation="MFSNNKNLHLTVQERIIIEKGIENGSTKAAIALTIGKDKSTVGK
                     EIKKHRELVHKSSYKINCANMKNCSHNHVCDNCADFKPFTCNRRDRSPGACNGCSKYT
                     HCRYDKYRYKADFSHKKYREDLVDSRTGINMSYEECKAMADIIVPLIKAGHSPYHIVT
                     NHPELNISEKTLYNYIENGIFREFGLLDIDLRIKTKRKITKKASNKYKKREDKKYLNG
                     RTYDDFINYTAENKNLSVVEMDTVYNNGSTGPFMQTFKFLDYSFMFIVYQEEKTAKSM
                     VEGVDFLEKILGEDLFSEEVAIIKTDRGSEFCDAEGFEKEENESRRTRIFYCDPMASG
                     QKGSLENNHKEIRYICPKEDDLKDLGLNSQEKANLIVSHINSQSKEHLKGKSPLEVME
                     FMNPALYQKFKDFGIERINKDNIVLKPYLLKD"
BASE COUNT          910 a          472 c          384 g          888 t
ORIGIN      
        1 ctgatacgat tgggagtcct gttgcttctt tagctgctac tagaatatct aaccctgctg
       61 atcccattcc ttggaaagca tatggtgatg ttcttggttt gaatgctcca cctcttaata
      121 gattagctcc tgctgctttg gctgctttag cgatttcaat cacttgttcc ttgctttcta
      181 ctgaacatgg tccagcaatc actgctagat gtcctccacc aattttaacg ccaccgacat
      241 cgataactga gtcttctgga tggaaagctc tattagctaa tttatatggt tctgatacat
      301 gcatgacctt ttctacagct ggatcaactt ctatcgattc tggatctact tttgttgtat
      361 ctccaataat tccaataact gattgtgttg ccccatttac aacatttact cttaacccta
      421 atttttctac tgatcctgat actttttcta catcttcttt agatgctgtt gatttcatta
      481 caataatcat attacttgtc cccttttctt catttccctt gatataaata aaaaagctct
      541 catcctctaa ggacgagagc tatcgcttcg tgataccacc tttgtttatt agtatattac
      601 tatactaacc tcatcgagta caatcatact cttccacttt aacgggtgta accggagatg
      661 cctacttaaa annnnnnnnn naaactgttc aacactctac tccaagatga attcaacaaa
      721 aatctctact ttccttttca cctaccagga aatctctacg ctaaagatca acgtctactc
      781 tttcttttca cagtatttat atcaaatgtg attacattgt atacaaattg ttatcatctg
      841 tcaaccattt gttttaaata taataaaaac tctaaagtga aaagttaaat tccactttag
      901 atatgacaaa gtggatttta gtctttcaca agcttccagt tgaatttact cttacagttg
      961 aacttacttt tacacttctt ttgtggaatt tactctttca cctgtataat ttacctgata
     1021 tctgttttcc attttcatga aaggtagatt atcatgtttt ctaacaataa aaatttacat
     1081 cttactgttc aagaaagaat catcattgaa aaaggtatcg aaaacggctc tactaaagct
     1141 gctatcgctc ttaccatcgg caaggacaag tctactgttg gtaaagaaat caaaaaacat
     1201 agagaacttg ttcacaaatc ttcttataaa attaattgtg ctaatatgaa aaactgttct
     1261 cacaatcatg tctgtgacaa ttgtgctgat tttaaacctt tcacctgcaa tcgtcgtgat
     1321 cgttctcctg gtgcatgtaa cggctgttct aaatatacgc attgtcgcta tgataagtat
     1381 agatacaagg ctgatttttc tcataaaaaa tatagagaag accttgttga ttctagaact
     1441 ggtatcaata tgtcatatga agaatgcaaa gctatggctg atattatcgt tccgcttatt
     1501 aaagccgggc attctcccta tcatatcgtt accaatcatc ctgaattgaa tatttctgaa
     1561 aaaacgctct acaactatat tgaaaatggt atctttagag aatttggact tctcgatatc
     1621 gatcttagaa tcaaaacaaa aagaaaaata accaaaaagg cttctaataa atataaaaaa
     1681 agagaagata aaaagtattt gaatggtcgt acatacgatg actttattaa ttatacagca
     1741 gaaaataaaa atctaagcgt tgttgaaatg gatacggtct acaataatgg ttctacaggt
     1801 cctttcatgc aaacatttaa atttttagac tattctttca tgtttatcgt ctatcaagaa
     1861 gaaaaaacgg cgaaatcaat ggtcgaaggc gtcgattttt tagaaaagat cttaggggaa
     1921 gaccttttca gtgaagaagt tgccattata aagactgata gaggaagtga attttgtgat
     1981 gctgaagggt ttgaaaaaga agaaaatgaa tcaagacgta cacgaatctt ctattgtgat
     2041 ccaatggctt ctggtcaaaa aggaagttta gaaaacaatc ataaagaaat ccgctatatt
     2101 tgtccaaaag aagatgattt aaaagattta ggacttaaca gtcaggaaaa agcaaatcta
     2161 atagtctcac atatcaattc tcaatcaaaa gaacatttaa aaggtaaatc accattagaa
     2221 gtaatggaat ttatgaatcc tgcactttat caaaaattca aagattttgg aattgaaaga
     2281 atcaacaaag acaatattgt tttaaaacct tacttattaa aagattaaaa tcattcagag
     2341 aatgtatgaa aatgggtata cagattgaca gttcaatact ttattcaagt ggattttagt
     2401 cttacactca aaaaaaattg acaaaaatgc acagctttaa tgtgccccaa aatagtactc
     2461 tttcataaca aaatttggtt ttcaatcaca tgaaagacta aattcaacca tcaaaataaa
     2521 aagaatatta ggcaaatcac ctaatattct tataaaatct gctttctgaa agagtaaatt
     2581 ctacttttat ttttcatttt tttaaagtgg aatttaactt ttcactttag caaaaaataa
     2641 aaaaaagaaa tattatcttt ttac
//
LOCUS       DBMDWL010000235         2637 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_18261_length_2637_cov_2.126577, whole genome shotgun sequence.
ACCESSION   DBMDWL010000235 DBMDWL010000000
VERSION     DBMDWL010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18261_length_2637_cov_2.126577"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="ACZPDN_11330"
     CDS             <1..827
                     /locus_tag="ACZPDN_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1299244.1"
                     /translation="FMSVVTLSNNFIDCLYTMASYYGNFVAIKGINDRLIRMIDKKET
                     EKTLLISENQNKVCFEHVSFGFPNKEAIFKDFSFQFDKGKKYAIVGDSGSGKSTLLKL
                     ILGYYSIQKGRIISFDKEPVIIRQEEHLFSGTIRENLCLGENFSEEDLTSAMEFANIA
                     DLDLNQFVSEDAGNLSGGQIQRISIARAKLHCHNLLLCDEITSSLDSINTEKIERNII
                     GLTDKTIIYVTHKIHKKMLQGFDDILVLSHGKLVEHGSFEDLLSKKNYFYHLFMNQ"
     gene            1080..2084
                     /locus_tag="ACZPDN_11335"
     CDS             1080..2084
                     /locus_tag="ACZPDN_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299245.1"
                     /translation="MKLFKYILCLFCAISLTACHQDEKQDFWEATIDAVQSKSKDNAY
                     LKNNWNIGISSDEGKKHQNMAARYIVENDNETTTTIFALQNQNDKEYISYTYTTDVIE
                     DTKEYQKTITINSTNKKYTKYDIQYNYYEFENGNYTYGEDATGSISINKDGITYDNVP
                     LLNEAIQSCCTIIDDFQEEFDIDYEEYDFDPLPYQMKDLNIPSIDEIQEETATSTDYY
                     GEQRINAKGYTLVDCLSIDKDTNEATYSTFNYERQSDEESIPCTLSLQGNNIYLLTPD
                     MDIDFTYYIYKTDDTVYMYSIDYSSNEIIEDITNNGGNMAEQVLKNTNDSLRKKIAEQ
                     "
     gene            complement(2191..>2637)
                     /locus_tag="ACZPDN_11340"
     CDS             complement(2191..>2637)
                     /locus_tag="ACZPDN_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013753.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1299246.1"
                     /translation="IHPDINKAWELKDEVTDFYVSNTIKTAPEAIETVIKHLRESDIE
                     ELVAFSKTLSNWKVEIINSFCISKAEYNVSKDTREITVEQKRINNALMENRNAIIKLV
                     TKAANGYRNWERFRNRCMLVLEKCIDFEIDKDDGSVKMVEKMDPDN"
BASE COUNT          939 a          407 c          439 g          852 t
ORIGIN      
        1 atttcatgtc tgtggtgaca ctttcaaata attttattga ttgcttgtat actatggcct
       61 cgtattatgg aaatttcgtg gctattaaag gcattaatga tagattgatt agaatgattg
      121 ataagaaaga aaccgagaaa acacttctta tttcagaaaa tcaaaataaa gtttgttttg
      181 aacatgtgag tttcggattt ccaaacaaag aagctatatt caaggatttt tccttccaat
      241 ttgataaagg aaaaaaatat gcgattgttg gtgacagtgg atcaggaaag tcgactttat
      301 taaaattgat tttgggttat tattcaattc aaaaaggaag aatcatttca tttgacaaag
      361 aaccggtaat catccggcaa gaagaacatt tattttcagg aactattcga gaaaatttgt
      421 gtttaggaga aaatttttct gaagaagatt tgacaagtgc aatggaattt gcgaatattg
      481 cagatttaga tctgaatcaa tttgtttctg aagacgcagg caatttgtca ggaggacaaa
      541 tccaaagaat atctatcgca agggcaaaac tacattgtca taatttattg ttgtgtgatg
      601 agattacatc aagccttgac agtattaata ccgaaaaaat tgaacgtaat attataggat
      661 tgactgataa aaccataatt tatgtaacac ataagataca taagaaaatg ctacagggat
      721 ttgatgatat attggtttta tcacatggca aacttgtaga acatggatca tttgaagatt
      781 tgcttagcaa gaagaattat ttttaccatt tatttatgaa tcagtaatgg attgaattat
      841 tactgaatat tccatggtga cggagccacc caatccacaa agggagccac ttcaatttat
      901 gaaatgactc tctttatcag gtttggcttt ttatattctt gattttcatc tatttaagaa
      961 ctatagtatc catgaattag aaacataaat atatagatgt tcttttgctt gtatttaaat
     1021 acactaattc aagatattta tcatccaaaa ttgtttgata aagaaagaag gctttaatca
     1081 tgaaattatt caaatatatt ctctgcttat tttgcgctat atctttaaca gcttgtcacc
     1141 aagatgaaaa gcaagatttt tgggaagcta ccattgatgc tgttcaatcc aaatccaaag
     1201 ataatgcgta cttaaaaaac aactggaata ttggtataag ttcagatgaa ggcaaaaagc
     1261 atcaaaatat ggctgcccgc tatatcgtag aaaacgacaa tgaaacaact actaccatat
     1321 ttgctttgca aaaccaaaat gataaagaat atatatccta tacatataca acagatgtaa
     1381 ttgaagatac aaaagaatat caaaagacaa taactattaa ttcaacgaat aagaaatata
     1441 caaagtatga tatccaatat aattactacg aatttgagaa tggaaactac acatatggag
     1501 aagacgcaac tggctcaata tcaatcaata aagatggcat tacatatgat aatgttccac
     1561 ttttaaatga agcaatccaa agctgctgta caataattga tgatttccag gaagaatttg
     1621 atatcgatta tgaagaatat gattttgatc ctctaccata tcaaatgaag gatttgaata
     1681 ttccaagtat agatgagatt caggaggaaa ccgcaacttc tacagactac tatggagaac
     1741 aaagaatcaa cgcaaaagga tatacacttg ttgattgttt aagtattgat aaagatacaa
     1801 atgaagcaac ctattcaact ttcaattacg aaagacagag tgatgaggaa tcaattccat
     1861 gtacactttc attacaaggt aataacattt atctattgac gccagatatg gatattgatt
     1921 ttacttacta tatttataaa acagacgaca ccgtttacat gtattccata gactattcaa
     1981 gtaatgaaat cattgaggat attaccaaca acggcggaaa tatggccgag caagttttaa
     2041 aaaacacaaa tgacagctta cgaaaaaaga ttgcagaaca ataaaggcgc aataaatttg
     2101 gatggggtta ggtgaatttg ctctccaaga atttaagggg cggcatatac cccaaaacgg
     2161 tttatagctg atattgggct cagaccacat ttagttgtct gggtccattt tttcaaccat
     2221 tttaacgctt ccgtcatcct tgtcgatttc aaaatcaata catttttcta agaccaacat
     2281 acatctgttt ctgaatcgtt cccaattgcg atagccatta gctgcttttg taaccaattt
     2341 gatgatcgca ttacgattct ccatcagtgc attgttgatt cttttctgtt ctacagtaat
     2401 ttctcttgtg tctttcgata cattatattc agcttttgat atacagaatg agttaatgat
     2461 ttcaactttc caattactca atgttttact gaatgccacc agttcttcaa tgtcactttc
     2521 tctaagatgt ttgataactg tttctatggc ttcaggggct gttttgattg tattcgaaac
     2581 ataaaaatcc gtgacttcat cctttaattc ccaggctttg ttgatatccg gatgaat
//
LOCUS       DBMDWL010000236         2625 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_18377_length_2625_cov_2.547147, whole genome shotgun sequence.
ACCESSION   DBMDWL010000236 DBMDWL010000000
VERSION     DBMDWL010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18377_length_2625_cov_2.547147"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            380..1360
                     /locus_tag="ACZPDN_11345"
     CDS             380..1360
                     /locus_tag="ACZPDN_11345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIR1299247.1"
                     /translation="MKYPKFLNENNNTIGVCAMSSGVGHKLDSFDASIEYIQSNGFHI
                     VETASVRNDSEPSCDALTRVDELNALVKDSSIGAVWLAAGGDFQFETLPYIDFDWIEK
                     NPKWYLGASDPTNLLFPVTCKCDLATVYGFNAGSFDAYGINEYSNLCLDFLKGKNCEL
                     HSSSMHQDVDFYNDGAPILNTKTEYVGSCSVVGRLLGGCFESINDMAGTPYDYVNEFN
                     ERYKEDGVIWFFDIFSMNSCDVYRALLKLKYMGYFKYTKAILVGRVLFKNVSDLIGYD
                     QAFKKALPDIDVVFQMDIGHTYPHMFVVNGAMARVEICDGHGLIQYEMKE"
     gene            1367..1777
                     /locus_tag="ACZPDN_11350"
     CDS             1367..1777
                     /locus_tag="ACZPDN_11350"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1299248.1"
                     /translation="MIEYTEEKKFTQDEVQELFLSVNWVSGEYPKRLYKALMHSSTVI
                     SAWVDGKLCGLVRVLDDSEMLAYVHYVLVNPKYQGLGIAGHLIELVKEKYKDYLYIEG
                     MPEDKSNVAFYVKHGFQEFEDAGSIIICNPGKKE"
     gene            1907..2590
                     /locus_tag="ACZPDN_11355"
     CDS             1907..2590
                     /locus_tag="ACZPDN_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299249.1"
                     /translation="MVVMDLKLDNLCAFRDFHVNFSYPKKIVNSYIEQEHLEGLPNFR
                     YKKVNIIMGSNATGKTSLGRLILSIFILIGKRNVEIITSYIADKDKKASFSMDFVNKD
                     HNIYKLYRINAEFDPSKNHDYSSENIKIRIESTDIKQRENYETCAKRLDSIMTGFTDA
                     NYIMELEKLKNLSWLFSFPFDAFFETRYRIEEDKEYLSVLNHTLKVLDPAIKGIIKLN
                     ELERKETSY"
BASE COUNT          843 a          266 c          574 g          942 t
ORIGIN      
        1 ttattacact taaattgtac atctaatgca atgcattgtc aatctaatag atatatatta
       61 tatacaaaaa aactgaatta ttttgtagga atacactaat gactatattt gatgcgagaa
      121 taataaatgt aactatctaa tacgtaataa agtaaataga tttttacttc tgcaatttgt
      181 ttgctttgat ttgtatttaa ttaaacatga aatacaaatt cactaaaaag tacaaagaca
      241 aatggattcg ttttgactat aataaaagtg tattggggca atactaaaac ttcctttcta
      301 tcttaaaact attgggaatg cggcaggact ttgtgtcttg tcgtttttcc tttataatgg
      361 atgtggaggt tttttgttta tgaaatatcc taagttttta aatgagaata ataatacgat
      421 tggtgtttgc gctatgtcga gtggtgtagg gcataagtta gatagttttg atgcgtctat
      481 agagtatatt caatccaatg ggtttcacat tgttgaaact gctagtgttc gtaatgatag
      541 tgagccaagt tgtgatgcct tgacgcgtgt tgatgaattg aatgcattgg tgaaggattc
      601 gagtattgga gctgtatggt tggcggctgg tggtgatttt caatttgaaa cgttgccata
      661 tattgatttt gattggattg aaaagaatcc gaagtggtac ttgggtgcga gtgatcctac
      721 aaatttatta tttcctgtaa cttgtaaatg tgatttggct acggtttatg ggtttaatgc
      781 gggtagcttt gatgcgtatg gaatcaatga atattccaat ttgtgtcttg attttttaaa
      841 gggaaagaat tgtgaacttc attcaagttc aatgcatcag gatgttgatt tttataatga
      901 tggtgctcct atacttaaca caaagacaga atatgtgggt agttgtagtg ttgtgggaag
      961 attactaggt ggatgttttg aaagtatcaa tgatatggcg ggcacgcctt atgattatgt
     1021 aaatgaattt aatgagcgtt ataaagagga tggtgttatt tggttttttg atattttttc
     1081 aatgaattca tgtgatgtat atcgtgcttt attgaagttg aagtatatgg ggtattttaa
     1141 atatacaaag gctatcttgg ttggtcgtgt actgtttaaa aatgtttcgg atttgattgg
     1201 gtatgaccag gcttttaaaa aggcactacc ggatatagat gttgtatttc aaatggatat
     1261 tggacatacg tatccgcata tgtttgtagt gaatggtgcc atggctagag ttgagatttg
     1321 tgatggtcat ggattgattc aatatgaaat gaaagagtga ttttaaatga ttgagtatac
     1381 agaagagaag aagtttacac aggatgaagt acaagaattg tttttgtctg tgaattgggt
     1441 gtctggtgaa tatcccaagc gattatataa ggctttaatg cattcgagta cagtgattag
     1501 tgcttgggtg gatggtaagc tttgtggttt agttcgtgtt ttggatgata gtgagatgct
     1561 ggcatatgtg cattatgtac ttgtgaatcc aaagtatcag ggtttgggta ttgcaggtca
     1621 tttgattgaa cttgttaaag agaagtataa ggattatcta tatattgagg gtatgcctga
     1681 agataagagc aatgtagctt tttatgtgaa acatggtttt caagagtttg aggatgctgg
     1741 tagtatcatt atttgtaatc cgggtaaaaa agaatagttt aaatctccat atctataaaa
     1801 aggtatgggg atttttcttt tctggttgag aaaaattaaa atcaagtata tactgatatt
     1861 agttcttaaa tatagaagta atataaacaa agaggggctg agaaatatgg tagttatgga
     1921 tttaaaattg gataatttat gtgcgtttag agattttcat gtgaattttt cgtatccaaa
     1981 aaagattgtg aattcatata ttgaacaaga acatttggaa ggattgccta attttcgcta
     2041 taagaaagta aatattatta tgggttcaaa tgcgacagga aagacgtctt taggccgttt
     2101 aattctttct atatttattt tgattggaaa aagaaatgta gaaattatta cgtcatatat
     2161 tgcggataaa gataaaaaag cttctttttc gatggacttt gtgaataagg atcataatat
     2221 ttataagtta tatagaatta atgcagaatt tgatccgagt aaaaatcatg attattcttc
     2281 tgagaatatt aagattcgaa ttgagtcgac agacattaaa caaagagaaa attatgagac
     2341 ttgtgcaaaa agattagatt ctattatgac cggttttaca gatgcgaatt atattatgga
     2401 attggaaaaa ttgaagaatt tatcatggct attttcattt ccatttgatg cattctttga
     2461 aactagatat cgtattgaag aggataaaga atatttgagt gtgttgaatc atactttaaa
     2521 agtattggat cctgcaatta aggggattat aaaactaaat gaacttgaaa gaaaagaaac
     2581 ttcgtattga agatgcattg aactcaactc gtgctgccgt tagtg
//
LOCUS       DBMDWL010000237         2565 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_19024_length_2565_cov_1.834821, whole genome shotgun sequence.
ACCESSION   DBMDWL010000237 DBMDWL010000000
VERSION     DBMDWL010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19024_length_2565_cov_1.834821"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="ACZPDN_11360"
     CDS             complement(<1..859)
                     /locus_tag="ACZPDN_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HIR1299250.1"
                     /translation="MLIKLLRPIKKFAGKCIRLAYKLTYRLFKVDDKLVIFISFHGRG
                     YSDNPKAIYEQMRQDPRFVGYRFIWFIKNHKEKNIHIEGAEIKEYFSIPYFYYLSKAK
                     LWVVNCKLPTYIFKKENQVYLQTWHGTPLKRLAHDIDVSEDTTFYRSGVSYEQMCHSY
                     DVDVARYNYMISPNAFCTRVFQTSFKINRERLIETGYPRNDFISNATKEECDAIKNKY
                     HLPMDKKIVLYAPTWRDNSYVSAGYTFELKANFRKWKEILGEDTIVVFKPHYLIINTF
                     KDDPELDGFL"
     gene            complement(877..>2565)
                     /locus_tag="ACZPDN_11365"
     CDS             complement(877..>2565)
                     /locus_tag="ACZPDN_11365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HIR1299251.1"
                     /translation="SFTVLGNLYNRDFVIRNNLKFNENFRYYCDMTFMCPLLEKLTSA
                     LQVEEAIYVKRKHNDPINEPALSQEENENRFNERCDAVEYTRTLLKEEGVVRFCLDRK
                     IVSYFVSQMAKKIRRSQDDLWRTDYFERIAKTIDGIRPEVMNRYKRNSRKMVAYLQNR
                     DLKGVQSCVRRKLGIKKFFRIFKDKNVLFKLAYYHVYSKKPIQENTILFETFMAKNYS
                     DSPKYIYEYIAKNYPGKYKCVWALNDGHEVPYGAEVVKRFSFKYAYYLAVSKYFVFNV
                     RQPLWYRKREGQVFVETWHGTPLKRLVFDQEEVTSASPKYKQQFYRQRQEWDYLVSAN
                     PFSTKTFRSCFMYEGKMLEYGYPRNDILYWPNKDQIAKDLRKKLGIPEDKKTILYAPT
                     WRDDEHYGKGEYKFTLALDLKLMMEKLSDEYVVLLRTHHYIADNIDTTGLEGFVYNLS
                     KYDDISEIYLISDICITDYSSVFFDFANLKRPVLFYTYDIEKYKNQLRGFYIDMNTEV
                     PGPLLYTSEEVVNAILHIDQINEEYKERYEEFYKRFCCYDDGNASKHIVEEVFK"
BASE COUNT          840 a          471 c          319 g          935 t
ORIGIN      
        1 ataagaatcc atctaattct ggatcatctt taaatgtgtt aataattaaa taatgaggtt
       61 taaatacaac aattgtatct tcgcctaaaa tttctttcca ttttctaaag tttgccttca
      121 attcaaaagt ataacctgca gaaacataag aattatctct ccatgtaggt gcatataaca
      181 caatcttttt atccataggc aaatgatact tattttttat tgcatcacat tcttcttttg
      241 tggcatttga aataaaatca tttctaggat aacctgtttc aatcaatctt tctctattga
      301 ttttaaaaga tgtttgaaaa acacgcgtac aaaacgcatt cggactaatc atataattat
      361 atcttgcgac atctacatca taagaatggc acatctgttc ataactaact ccagatcgat
      421 agaacgttgt atcttcgctt acatcaatat cgtgagctaa tcgttttaac ggggttccgt
      481 gccatgtttg tagataaacc tgattttcct ttttaaagat atatgtaggt aatttgcagt
      541 tcactaccca taattttgcc ttggacaaat aatagaaata aggaatgctg aaatattcct
      601 ttatttcagc tccttcaata tgaatattct tttctttatg attttttata aaccaaataa
      661 aacggtatcc aacaaatcgt ggatcttgtc tcatttgttc ataaatagct ttaggattat
      721 ccgaatagcc acggccatga aaagatataa atatgacaag cttatcatct actttaaata
      781 gacgatatgt taatttatag gccaagcgaa tacactttcc tgcaaacttt ttaataggtc
      841 ttaaaagctt aattaacatg gctatccccc cttcttttat ttaaatactt cctctacaat
      901 atgctttgac gcatttccat cgtcataaca acagaaacgc ttgtagaatt cttcataacg
      961 ttctttatat tcttcattga tttgatcaat atgtaaaatc gcattcacaa cttcttcact
     1021 tgtatataat aatgggcctg gaacctcagt attcatatca atatagaatc ctcgtaattg
     1081 atttttatat ttttcaatat catatgtata gaaaaggact ggtcgtttta aattcgcaaa
     1141 atcaaagaat acacttgaat agtctgtaat acaaatatca ctaatcaaat agatttcact
     1201 aatatcatca tatttgctta aattataaac aaaaccttca agtcctgttg tatcaatgtt
     1261 atccgcaata taatggtgcg ttcttaataa aacaacatat tcatctgata atttttccat
     1321 cattaacttc aaatctaagg ccaatgtaaa tttatattca ccttttccat aatgttcatc
     1381 atcacgccat gttggtgcat ataaaatcgt tttcttatct tctggaatac ctagtttttt
     1441 tcttaagtct ttcgcaattt gatctttgtt tggccaatat aaaatatcat ttcttggata
     1501 tccatattca agcattttac cctcatacat aaaacaactt ctaaatgttt tcgtactaaa
     1561 cggattggca cttactaaat aatcccattc ttgtctttgg cgatagaatt gctgcttata
     1621 ttttggagat gctgaagtta cttcttcttg atcgaacaca agacgtttta atggtgtacc
     1681 atgccaagtt tcaacaaata cttggccttc tcttttgcga taccacaatg gttgtctaac
     1741 attaaataca aaatatttac taacagctaa ataatacgca tatttaaaag agaaacgctt
     1801 tacaacttct gctccataag gaacttcatg cccatcattc aatgcccata cacatttata
     1861 tttgcctgga taattcttgg caatatattc atatatatat ttagggctgt ctgagtaatt
     1921 tttagccata aatgtttcaa ataaaattgt gttttcttga attggtttct tagaataaac
     1981 atgatagtaa gcaagtttaa ataaaacatt cttatcttta aagattctaa aaaacttttt
     2041 aattcctaat tttcttcgaa cacaactttg gacacctttc aagtcacgat tttgtagata
     2101 agctaccatt tttctagaat tacgcttata tcgattcatc acttctggac gaatgccatc
     2161 gattgtttta gcaattcttt caaaatagtc tgtacgccat agatcatctt gactacggcg
     2221 aatcttcttt gccatttgag aaacaaaata tgatacaatt ttacgatcta agcagaaacg
     2281 tacaacccct tcttctttta aaagtgtacg tgtatattca accgcatcgc atctttcatt
     2341 aaaacgattt tcattctctt cctgactcaa tgctggttca ttaattggat cattatgctt
     2401 acgtttcaca tagattgctt cttctacttg taaagcggaa gtcaattttt ctaataatgg
     2461 acacatgaat gtcatatcac aataatatct aaagttctca ttaaacttta agttattgcg
     2521 aataacaaaa tcacgattat acaaattacc taacactgta aagct
//
LOCUS       DBMDWL010000238         2558 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_19116_length_2558_cov_2.330891, whole genome shotgun sequence.
ACCESSION   DBMDWL010000238 DBMDWL010000000
VERSION     DBMDWL010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19116_length_2558_cov_2.330891"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="ACZPDN_11370"
     CDS             <1..979
                     /locus_tag="ACZPDN_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HIR1299252.1"
                     /translation="RELVQYYFEQLKKLAKQDHCIFIKMDPAIHVNDYDSKSYNTNRY
                     EETETYLDVFKSCKAIHKGYTMHISDTVQPRFQSNVYSYENIDETLPKHTKRLIKDAD
                     RRNVQIIHGQGEWLDEFSRLVELTESRKGVALRDKEYFKTLLENYPDGGVIFLAMCNV
                     YQLNREAEIKKVKLEKEIAQTCGNAKKKLHRLEDQLRSVDKDIKEFKEIFSEFGQENK
                     DIAIAEILSIQYGNTCEMLYAGMDERFKKFMPQYKEYVENFKWAFEKGCSWSNMGGVE
                     GTLDDGLTKFKDNFNPTINEFIGEFDIPIYPFMYRLTQKAYKILKSKHM"
     gene            1079..1213
                     /gene="rpmH"
                     /locus_tag="ACZPDN_11375"
     CDS             1079..1213
                     /gene="rpmH"
                     /locus_tag="ACZPDN_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR1299253.1"
                     /translation="MKRTYQPSKRKHQKVHGFRARMATVGGRNVLSRRRAKGRKVLSA
                     "
     gene            1258..1599
                     /gene="rnpA"
                     /locus_tag="ACZPDN_11380"
     CDS             1258..1599
                     /gene="rnpA"
                     /locus_tag="ACZPDN_11380"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR1299254.1"
                     /translation="MKKEFRVLKNYEFSSIIQNRQFVKSSSFVCYFQKRKLDHARVGI
                     SVGKKLGNAVCRNKVKRQLRSIVDDVFTFEEGYDVIIIVRPAYTNKSFEENKNEMKEN
                     QNRINKRFLKK"
     gene            1611..>2558
                     /locus_tag="ACZPDN_11385"
     CDS             1611..>2558
                     /locus_tag="ACZPDN_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR1299255.1"
                     /translation="MKQFLQKKAKLVFLCMLVLLTCTACANPRGRDGKTLADQIIASE
                     ETTIDASKINYKDISDKKLKKEIKKNIKDGQYTIQPTTFKDSMSKGWFEGLIVWPIAQ
                     IINLISSKTDAGVGIILTTLLIQMLVFVFTRKSQMSTQRMQEISPEIQKIQNKYKDKT
                     DERSKLQMAQEMQNLYQKYDIHPFGSMLITFIQLPIMMGMYYATMRSVSVVSGSFMGI
                     SLAGTPLDGFKALDIAYIAIYVLMVIFYIISMKLPQWLKKWQDKKDNVKVKKYAQEET
                     NPMANSMNMSMYFMTAMICIMYISWPMAMSFYWLVSSVIR"
BASE COUNT         1007 a          314 c          469 g          768 t
ORIGIN      
        1 tagagagctt gttcagtatt attttgaaca attaaaaaag ttagctaaac aagaccattg
       61 tatatttatt aagatggatc cagctattca tgttaatgat tatgactcaa aatcctataa
      121 cacaaatagg tatgaggaaa cagaaacata cttagatgta tttaaaagtt gtaaagctat
      181 tcataaagga tatacaatgc atatttcgga tacagttcag ccaagatttc aatcaaacgt
      241 atattcatat gaaaatatag atgaaacatt gccaaaacat acgaaacgtt taatcaagga
      301 tgcagaccgt agaaatgttc aaattattca tggacaaggt gaatggctag atgaattcag
      361 ccgacttgta gaattgacgg aatcaagaaa aggagtggca ttaagagata aagaatattt
      421 taaaacacta ttagaaaatt atccggatgg tggagtcatc tttttagcta tgtgtaatgt
      481 gtatcagttg aatagagaag cagaaataaa aaaggtcaaa cttgaaaagg aaattgctca
      541 aacttgtgga aatgcaaaaa agaaattgca tcgtttagaa gatcaattaa gaagtgtaga
      601 taaagatata aaagaattta aagaaatatt ttctgaattt gggcaagaaa acaaagatat
      661 cgcaattgca gaaatactta gcattcaata tggaaatact tgtgagatgt tatatgcagg
      721 aatggatgaa agatttaaaa agttcatgcc acaatacaaa gaatatgttg aaaactttaa
      781 atgggcattt gaaaaaggat gttcatggag taacatggga ggtgtagaag gaactttaga
      841 tgatggatta acaaaattca aagacaattt caatccaaca atcaatgagt ttattggtga
      901 atttgatata ccaatttatc cattcatgta tcgtttaaca caaaaagcat ataaaatact
      961 gaaaagtaag catatgtgat aattatttta ttgacaatta ttttatttta tgtttataat
     1021 gtttgagcaa tcttatttga aaatttagaa gaagaaaaga ctgagaggtg taatcaagat
     1081 gaaaagaaca tatcagccaa gtaagagaaa acaccaaaaa gttcatggtt tccgtgctcg
     1141 tatggcaact gttggtggac gtaacgttct ttctagaaga agagcgaaag gcagaaaggt
     1201 tttatccgca tagaaatcac ctgctaggtg atttttttgt ctggataggg aaataacatg
     1261 aaaaaagagt ttcgtgtatt aaaaaactat gagttttctt ccatcatcca gaatcgacaa
     1321 tttgttaagt cgtcgtcatt tgtatgttat tttcaaaaac gaaaattgga ccatgcaaga
     1381 gtcggaatca gtgttggcaa aaaattagga aatgcggttt gtcgaaataa agtgaaacga
     1441 caactacgta gtatcgttga tgacgtattt acctttgaag agggatatga tgttattatt
     1501 atagttcgac ctgcctacac gaataaatcg tttgaagaaa ataaaaatga aatgaaagaa
     1561 aatcaaaatc ggataaataa aagatttttg aaaaagtaga ggaataaaaa atgaaacaat
     1621 ttttgcagaa aaaagcaaaa ttggtctttc tatgtatgtt ggtattatta acatgtacgg
     1681 cttgtgcaaa tccaagaggt agagatggaa aaacattagc agaccaaatt attgcaagtg
     1741 aagagacaac aattgatgca agcaaaatca attacaaaga catttctgat aagaaattaa
     1801 agaaagaaat taaaaagaat attaaagatg gacaatatac aattcaacca acaactttta
     1861 aagattctat gtctaaagga tggtttgaag gtttaattgt ttggccaatt gcacaaatta
     1921 ttaacttaat ttcttcaaaa acagatgcgg gtgtaggtat cattttaaca acattgttaa
     1981 ttcagatgct tgtatttgta tttacacgta aatcacaaat gtctacacaa agaatgcagg
     2041 aaatctcacc tgaaattcaa aagatccaaa ataaatataa agataaaaca gatgagcgtt
     2101 ctaaattgca aatggctcaa gaaatgcaaa atctttatca aaaatatgat attcatccat
     2161 ttggttcaat gttgattact ttcattcagt taccaatcat gatgggtatg tattatgcta
     2221 caatgcgttc tgtaagcgtt gtaagtggaa gctttatggg catttcttta gcgggtacac
     2281 cacttgatgg atttaaagct ttagatatcg catatatcgc aatttatgta ttaatggtta
     2341 ttttctatat tatttcaatg aaattacctc aatggttaaa gaaatggcaa gataagaaag
     2401 ataacgttaa agttaaaaaa tatgcacaag aagaaacaaa tcctatggct aacagcatga
     2461 atatgtcaat gtacttcatg acagctatga tttgtattat gtatatttca tggccaatgg
     2521 caatgtcatt ctattggtta gtatcaagtg taattcgt
//
LOCUS       DBMDWL010000239         2514 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_19597_length_2514_cov_1.731455, whole genome shotgun sequence.
ACCESSION   DBMDWL010000239 DBMDWL010000000
VERSION     DBMDWL010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19597_length_2514_cov_1.731455"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="ACZPDN_11390"
     CDS             <1..188
                     /locus_tag="ACZPDN_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299256.1"
                     /translation="INQGVQDLNRIDIDKLQDLPAWFDDLLHPYQHMTYYSDQRIHKL
                     CVLLNRQIHNLKFFLSS"
     gene            239..1102
                     /locus_tag="ACZPDN_11395"
     CDS             239..1102
                     /locus_tag="ACZPDN_11395"
                     /inference="COORDINATES: protein motif:HMM:NF025384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HIR1299257.1"
                     /translation="MFCKNCGHEISDDAKFCSNCGTKVEVEEEIVEPIEVVDKTQAKE
                     WYYVENNDSKGAFTLEEMKELIESNKITGSTLVWKASLKDWQKLADSELNEFIHVDEE
                     KNWYYVENNDSKGPYSQEEMKGFMDMGVLTGNSFVWKTGMQDWIHLKNTELAGNQMPE
                     QEPTPQPSYYGVKEKSIALNVVLTFVTCGLYGLYWLYTIAKDLNDLCVNQHQEKGADP
                     FLVVVLSIVTCGIYQIYYMYKAGKMVSSLTKNGQHLSDDSVVLMVLAILQLSLVSYCI
                     LQSNINGLTKN"
     gene            1083..1457
                     /locus_tag="ACZPDN_11400"
     CDS             1083..1457
                     /locus_tag="ACZPDN_11400"
                     /inference="COORDINATES: protein motif:HMM:NF022281.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR1299258.1"
                     /translation="MDSQKTKTLGIVLILFLLLLFYKCPIQSLTGLPCPGCNMKTSLY
                     YLVHFKFSLSLYYHALLIPTLIVFVVALLNLNKPERIKKLLIIWAILMIVYYIYRMIV
                     YFPNSPMIYSKENLIYFIFRHV"
     gene            1502..1795
                     /locus_tag="ACZPDN_11405"
     CDS             1502..1795
                     /locus_tag="ACZPDN_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HIR1299259.1"
                     /translation="MSNIYFTITGLNHYYGNEFLEKEMVVYLKKDNDNEYDKEAISVN
                     LAGLGKIGYVANSPYTVLGESYSAGRLYDKIEDETQGKIKFILDKGVVCELVE"
     gene            1829..2278
                     /locus_tag="ACZPDN_11410"
     CDS             1829..2278
                     /locus_tag="ACZPDN_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HIR1299260.1"
                     /translation="MWYQGDNKVLDEPKIYLDGSYKDFGYGFYCTNFEKQAKRWALTK
                     KNKHVVNVYSYTENKNLNCLIFKEMSDEWLDFVVSSRQGIRHEYDIVEGPMADDTIWN
                     YVNDFARGFISRTAFWELVKFKYPTHQIVFCTEAALKCLEYARSYEL"
     gene            2275..>2514
                     /locus_tag="ACZPDN_11415"
     CDS             2275..>2514
                     /locus_tag="ACZPDN_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299261.1"
                     /translation="MIIKYFDEPIVENDLFYICFLIEKVSRHLHQRNSYVVNKLGYDP
                     LYHLISVTNVLHCENSDQVVYDVIEEYGLKQGNFHI"
BASE COUNT          876 a          326 c          484 g          828 t
ORIGIN      
        1 aaattaatca aggcgtacaa gatttaaatc gcattgatat agataaacta caagatctac
       61 ctgcatggtt tgatgacttg ttgcatccat atcaacatat gacttactat tcagatcaac
      121 gtattcataa gctatgtgtt ttgctgaatc gacaaattca taatcttaag tttttcttaa
      181 gtagttgaga taacacaaga ttcatcttat aattgaatca atcgaaagga tgtttgttat
      241 gttttgtaaa aattgtggtc acgaaatcag tgatgatgca aagttctgtt caaattgtgg
      301 aacaaaagta gaagtcgaag aagaaattgt tgaacccatt gaggttgttg ataaaacaca
      361 agctaaagaa tggtattatg tagagaataa tgattctaaa ggtgcattta ctttagaaga
      421 aatgaaagaa ttgattgaat ccaataaaat tacgggttct actctagtat ggaaggcaag
      481 tttaaaagat tggcaaaaac tagcggatag tgagcttaat gaatttatac atgtggatga
      541 agaaaagaat tggtattatg ttgaaaacaa tgattccaaa ggaccttatt cacaagaaga
      601 aatgaaaggg tttatggata tgggcgttct tactggcaat agctttgtat ggaaaacagg
      661 tatgcaagat tggatacacc taaaaaatac agagcttgcc ggaaatcaaa tgccagaaca
      721 agaacctaca ccacaacctt cttattatgg ggtgaaagaa aagagtattg ccttaaatgt
      781 tgtattaacg tttgtgacat gtggactata tggcctttat tggctttata ccatcgccaa
      841 agatttaaat gatttatgtg taaaccaaca ccaagaaaaa ggagctgatc cattccttgt
      901 ggttgtatta agtattgtca cttgtggtat ttatcaaatt tactatatgt ataaagccgg
      961 taagatggtt tcaagcctaa ctaaaaatgg tcaacaccta agtgatgata gtgttgtttt
     1021 aatggtatta gccattctac aactatcatt agtttcttat tgcatcctac aaagtaatat
     1081 caatggactc acaaaaaact aagactttag gaatagttct tatactattc cttcttttat
     1141 tattctataa atgtcctatt caatcactta caggcttacc atgtcctgga tgcaacatga
     1201 agacaagcct atactatcta gttcatttta aattctcttt atccctctat tatcatgcac
     1261 tccttattcc tactcttatt gtgtttgtgg tggctttgct gaatttaaat aaaccagaaa
     1321 gaatcaagaa actattgatc atctgggcca ttttaatgat tgtctattat atctatcgta
     1381 tgattgttta ttttcccaat agtccaatga tatattctaa agaaaattta atttatttca
     1441 tatttagaca tgtataatgt caattaaagt tgtattgtga aatcaaaagg aggacaagaa
     1501 tatgtcaaac atttatttca cgatcactgg gttaaaccat tattacggaa atgagtttct
     1561 agaaaaggaa atggtcgttt atctaaagaa agataatgat aatgaatatg ataaggaagc
     1621 cattagtgtc aatcttgcag gtctaggaaa gattggctat gttgccaata gtccatatac
     1681 agtactagga gaaagctata gtgctggaag attatatgat aagatcgaag atgagacaca
     1741 gggtaagatc aagttcattc tagataaggg agttgtatgt gaacttgtag aataagggta
     1801 ttaaagaatt ggaataggta taggtaagat gtggtatcag ggtgacaata aagtattgga
     1861 tgagccaaaa atatatttag atggttcata caaagatttt ggatatggat tctattgtac
     1921 gaattttgaa aaacaagcta aaagatgggc gttaacgaaa aagaataagc acgttgtgaa
     1981 tgtatattct tatactgaaa ataagaattt aaattgtttg atttttaaag agatgagcga
     2041 tgagtggcta gattttgtag tttcatcaag acaaggtata agacatgaat atgatattgt
     2101 tgaagggcca atggcggatg atacaatttg gaattatgta aatgactttg cgcgtggatt
     2161 catttctaga actgcatttt gggagttggt taaattcaag tatcctacac atcaaattgt
     2221 attttgtaca gaagccgctt tgaaatgtct agaatatgct aggagctacg agttatgatc
     2281 atcaaatatt ttgatgagcc tatcgtagaa aatgatttgt tctatatttg ttttctaatt
     2341 gaaaaagtgt ctagacatct tcatcagcga aactcatatg ttgtgaataa acttggatat
     2401 gatcctttgt atcatttgat aagtgtcaca aatgtcttgc attgtgaaaa ttcagatcag
     2461 gttgtttatg atgtgattga agaatatggg ttaaagcagg gcaattttca tatt
//
LOCUS       DBMDWL010000240         2511 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_19637_length_2511_cov_1.429876, whole genome shotgun sequence.
ACCESSION   DBMDWL010000240 DBMDWL010000000
VERSION     DBMDWL010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19637_length_2511_cov_1.429876"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="ACZPDN_11420"
     CDS             <1..837
                     /locus_tag="ACZPDN_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299262.1"
                     /translation="ASKTASVKAGICAGTVAVTGAVGYAAVDYNSNYKQVERAIEKLD
                     ASHSYEIYEYTLGPTVKNDEYIGNYELNDRLYVENTDSPDVYSVIYYANPEAGVEQNL
                     FEKFDGKKMASCYSDANSFDELDPNLISEYKEPSFGEDGRPTSDAPSVGIWRLKYDTI
                     VKFDTYNENYLSLYNTYYKSLYGKDNIQTNLKKKYLKIAQEGLNNAKVTEKDGEKTFS
                     YQTYDLSIDEEGNIQENKDIIFNLTVTIDKDGYFKKYTNHYYIDYEYKNEITIGNILK
                     KQ"
     gene            939..>1094
                     /locus_tag="ACZPDN_11425"
     CDS             939..>1094
                     /locus_tag="ACZPDN_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299263.1"
                     /translation="MEKDLINRARKKDAQAISEIVQKTQRKAIYFAYKVVNDEALAKE
                     IVLQSYIL"
     assembly_gap    1095..1104
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1105..1886
                     /locus_tag="ACZPDN_11430"
     CDS             <1105..1886
                     /locus_tag="ACZPDN_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1299264.1"
                     /translation="LAFQNLDQLMDDGMFEHWFGRKVSEQLLNQIEYEKNSSLNSSFY
                     VDEEDRIEFDANVRNDKMPFKFESFMNVQKIQDTTQSVLNLLTEKQRLMLMMYYFENM
                     SIFEIADIVGVSENAVKDNLAYTRKKIMNTLSDANVKSMAFLSWFLNDQMSKMISCHV
                     SVDTIVEAISCIPQQVGIYDNMSGILGWWLRKTAISKALIVVLSMTLMSFILGALSDS
                     NYNTIVNATEKLDTLESFHIKGVQHTQNGDIDFDVSVEKYR"
     gene            2071..2475
                     /locus_tag="ACZPDN_11435"
     CDS             2071..2475
                     /locus_tag="ACZPDN_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299265.1"
                     /translation="MDCYILSSKSIYKELLTMQPDSYDIALLKVYGRLFSGDENLRIR
                     AVDKVRVLDEENINEANFERDGDIKRIKYNLALWKTDNSNEIITEQKSDSSNMVEIRD
                     GYITHLELNDPFDGGQYYYGSYLKMDFMDFVE"
BASE COUNT          954 a          268 c          472 g          807 t
ORIGIN      
        1 gctagtaaaa cggcgagtgt aaaagctgga atatgtgctg gaacagtagc tgtaactggt
       61 gcagttggat atgcagcggt tgattataat agtaactata aacaggtaga gagagctatt
      121 gaaaaacttg atgcatccca ctcttatgaa atttacgaat atactttggg tcctactgtt
      181 aaaaatgacg agtatattgg taattatgaa ttaaatgatc gtttgtatgt agaaaataca
      241 gattcaccag atgtttatag tgtaatttat tatgcgaacc ctgaagcagg tgtcgaacaa
      301 aacttgtttg aaaaatttga tggtaaaaaa atggcatcat gttattcaga tgcgaatagt
      361 tttgatgaat tagatccaaa tttaatttca gaatataagg agccttcatt tggagaggat
      421 ggaaggccaa ctagtgatgc tccttctgta ggtatatgga gattaaaata tgatactata
      481 gtgaaatttg atacttataa tgaaaactat ttaagcttat ataatacgta ttataaaagt
      541 ttatatggta aagataatat acaaacaaac ctgaagaaga aatatcttaa aatagcacaa
      601 gaaggtttaa ataatgcaaa agtaacagaa aaagatggag aaaaaacatt ctcttatcaa
      661 acatatgatc tatcaattga tgaagaagga aatattcaag aaaataaaga tattattttc
      721 aatctcacag ttacaatcga caaagatgga tactttaaga aatatacaaa tcattattat
      781 atagattatg agtacaaaaa tgaaataact ataggtaaca tactaaaaaa acagtgactc
      841 aattatagtt attaaattgt tttaagatgc aatcgaaaga ttgcatctgt attttcataa
      901 ataaaagaag tagggataca aggtgttaga ggagaaatat ggaaaaggac ttaattaata
      961 gagcaagaaa aaaggatgct caagccatta gtgagattgt tcaaaaaaca cagcgtaagg
     1021 caatttattt tgcgtataag gttgttaatg atgaagcgtt agctaaggag atagttttac
     1081 aatcttatat attgnnnnnn nnnntattgg cttttcagaa tttagatcaa ttgatggatg
     1141 atggaatgtt tgaacattgg tttggtcgaa aggtttcaga acagttattg aatcaaattg
     1201 aatatgaaaa gaattcatct ttaaattcta gtttctatgt agatgaagaa gatcgaattg
     1261 aatttgatgc caatgttcga aatgataaaa tgccgtttaa gtttgaatca tttatgaatg
     1321 ttcaaaagat tcaagatact acacaaagcg ttttaaatct cttaacggaa aaacaacgtt
     1381 tgatgttaat gatgtattat tttgaaaata tgagtatttt tgaaattgct gatattgttg
     1441 gtgtaagtga gaatgctgta aaagataatt tggcttatac cagaaagaaa ataatgaata
     1501 ctttaagcga tgcaaatgtt aaatctatgg catttttaag ttggttttta aatgatcaaa
     1561 tgtcaaaaat gatttcatgt catgtgtctg tggatactat tgttgaagca ataagttgta
     1621 tacctcaaca agtaggaata tacgacaata tgtctggtat ccttggctgg tggcttcgaa
     1681 aaacagcgat ttctaaagct ttgattgttg ttctttcaat gactctaatg tcatttattt
     1741 tgggtgcgtt aagtgatagc aactataata caattgtaaa tgctacagaa aagttggaca
     1801 ctcttgaatc gtttcatata aaaggagtgc agcacacaca aaatggtgat attgattttg
     1861 atgtttcagt agaaaaatac agatgatata gatgagtatt atgcggttgc tacaactaca
     1921 attcctgaag aagttggtta ttattatgtt ttccattata aagataatca atgccaaaag
     1981 tatataacga ctacacctgt tgatttaaat tcaatagatg gaagtcaatt tggagaatat
     2041 ttaacaccat caaccaatgg aaatgatctt gtggattgtt atatactttc ttcaaaatct
     2101 atatataaag agctattaac aatgcaacct gacagttatg atatagcgct tttgaaagta
     2161 tatggtagat tatttagtgg agatgaaaat ttgaggatta gagctgtaga taaagtacga
     2221 gttttagatg aggaaaatat aaatgaagct aactttgaaa gagatggaga tataaagaga
     2281 ataaagtata atcttgcatt atggaaaaca gacaattcaa atgaaataat tacagaacaa
     2341 aaatcagatt caagtaatat ggttgaaatt cgtgatggat acataacaca tttagagtta
     2401 aatgatccgt ttgatggtgg acaatattac tatggtagtt atttaaaaat ggattttatg
     2461 gactttgttg aatagataca gaaaagtatc tatttttttt tatgctaacg a
//
LOCUS       DBMDWL010000241         2494 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_19821_length_2494_cov_2.120769, whole genome shotgun sequence.
ACCESSION   DBMDWL010000241 DBMDWL010000000
VERSION     DBMDWL010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19821_length_2494_cov_2.120769"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            107..967
                     /gene="rapZ"
                     /locus_tag="ACZPDN_11440"
     CDS             107..967
                     /gene="rapZ"
                     /locus_tag="ACZPDN_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR1299266.1"
                     /translation="MENKIVLVTGMSGAGKSSAMNALEDLGYYCMDNFPKELLDNLEE
                     LLRKDETHYKNVALSVSAIDYQAFVSYFENINRELQILFLDCSDEELLLRYRFTRRQH
                     PMLVNHLATTLEDAIEVERDFLDHLQENSQNTIHIDTTKLSTSALASRVLHRFKSAKR
                     SVFTVTFQSFGFKHGVPLDADMVIDVRFLPNPFYDPLLREKTGNQKEVYDYVMDKIET
                     QEFIVHLKNYCDFVFNQYEKQNKSHMIVAIGCTGGQHRSVSICNWLYDTYKDTYRCFK
                     SHRDIGVDEE"
     gene            964..1911
                     /locus_tag="ACZPDN_11445"
     CDS             964..1911
                     /locus_tag="ACZPDN_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR1299267.1"
                     /translation="MKKIVVIGGGTGLSSMLKGMKRLENVDLTAIVTVADDGGSTGRI
                     RDIYNVPAVGDIRHVLCAMADEEDEGFFSDLLNYRFEGSKDIGGHNLGNLIFLAMINT
                     TGSFMGAIESISRVLNVKGNILPSTLDVVTLYAMMKDGTLVRGEKNIPTVYNSIDHVF
                     YQTDVQAYKPAIDAIENADLIIYGIGSLYTSIMPNLIISDISKAIEKNPCKKIYFCNA
                     MSQPGETDGYTVQDHIRAIEKHSFKHPVDLVVVNQSKLPKEVLDMYASQKSYPICIGN
                     EATDYAIIQRDLLALDSKGRIRHNPMAVKQVVEELIKGV"
     gene            1914..>2494
                     /gene="whiA"
                     /locus_tag="ACZPDN_11450"
     CDS             1914..>2494
                     /gene="whiA"
                     /locus_tag="ACZPDN_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR1299268.1"
                     /translation="MSFTSEIKKEICKEEIDAQMMKAQLCALFQMRASLHMNWQGMYL
                     SFQTENATIAKHVFQIMKTLYNVDPRLSVLKKMQLKKNNIYRIQVFDKAQEILEDLQI
                     LTDSGLRYTPSVKMVRSEKMARAYLQGCFLASGSINSPKSSNYHLEMSSQEKDLALSV
                     QKLMQRFYLPAKVIERKSLYVVYIKAGDKIADF"
BASE COUNT          851 a          322 c          529 g          792 t
ORIGIN      
        1 aacaacaccg ccttcaacca cggttattaa aggttgaaag aaaggagaag tatattgtag
       61 tcgaccagtt agatgggtca attacaatat accaggatgt gggattatgg aaaataaaat
      121 tgttcttgtg acaggaatgt caggagctgg aaaatcaagt gcaatgaatg ccttggaaga
      181 tttagggtat tattgtatgg ataattttcc taaggaatta ctagacaatt tagaagaatt
      241 attaagaaaa gatgaaacgc attataaaaa tgtggcatta agtgtgagtg caatagatta
      301 tcaagctttt gtcagttatt ttgagaatat aaatagagaa ctacaaattt tatttttaga
      361 ttgtagtgat gaagaattgt tgttgcgcta tcgctttaca agaagacaac acccaatgct
      421 tgtaaatcat ttagctacga cattagagga tgcgattgaa gttgaacgtg attttttgga
      481 tcatctacaa gaaaattcac agaatacgat tcatattgat acgacaaaat taagtacttc
      541 agctttagca agtcgtgttt tacatcgttt taaaagtgca aaacgaagtg tatttacagt
      601 aacatttcaa tcttttggat ttaagcacgg ggtgccatta gatgcagata tggttattga
      661 tgttcgtttc ttacccaatc cattctatga tcctttgttg agagaaaaaa caggaaatca
      721 aaaagaagtt tatgattatg ttatggataa aatagaaaca caggaattta ttgttcattt
      781 aaaaaattat tgtgattttg tatttaacca atatgagaaa caaaataagt cacatatgat
      841 tgttgcgata ggttgtacag gaggacaaca tcgttcggtt tccatttgta attggttata
      901 tgacacatat aaagatacat atcgttgttt taaatcacat cgcgatattg gagtggatga
      961 agaatgaaaa agatagttgt gattggtgga ggtacaggtc tatccagcat gttgaaaggc
     1021 atgaaacgat tggaaaatgt ggatttaacg gccattgtta cggttgcgga tgatggtggt
     1081 agtacaggaa gaattcgaga tatttacaat gtacctgcag ttggagatat acgacatgtt
     1141 ttatgtgcga tggccgatga agaagatgag ggtttctttt cggatttatt aaattatcgt
     1201 tttgaaggta gcaaagacat tggtggacat aatctaggaa atctgatctt tttggctatg
     1261 atcaacacga caggttcttt tatgggtgct attgaatcta tttcaagagt tttaaatgtg
     1321 aaaggaaata ttttgccgag tacattagat gttgtcactt tgtatgccat gatgaaggat
     1381 gggactttgg ttcgtggcga aaaaaatatt ccaactgtat acaatagtat tgatcatgtg
     1441 ttctatcaaa cggatgtcca agcttacaag ccggctattg atgcaattga aaatgccgat
     1501 ttaattatat atggaattgg atcgttatat acttcgatta tgccaaactt gattatttca
     1561 gatatatcta aagcaataga aaagaatcct tgtaaaaaga tttatttctg taatgcgatg
     1621 tcgcaacctg gagaaacaga tggctatact gtacaagatc atattcgtgc cattgaaaag
     1681 cattcattta aacatccggt ggatttagtt gttgtgaatc aatcaaagtt accaaaggaa
     1741 gtattggata tgtatgcttc acaaaagagt tatccaattt gtattggaaa tgaggcaacg
     1801 gactatgcta ttatacaaag agatttgttg gccttggact caaaaggacg tattcgtcat
     1861 aatccaatgg cggttaaaca agttgttgag gaactaatca agggggtata gacttgtcct
     1921 ttactagcga aatcaaaaag gaaatttgta aagaagaaat cgatgctcaa atgatgaagg
     1981 cacaattatg tgcccttttt caaatgcgag caagtttaca tatgaactgg caaggtatgt
     2041 atttatcttt tcagactgaa aatgcaacga ttgcgaaaca tgtatttcaa attatgaaaa
     2101 cattatataa tgtggatccg cgtctatctg tattgaaaaa aatgcagtta aagaaaaaca
     2161 atatatatcg tattcaggtt tttgataagg cacaggaaat tttggaagac cttcaaattc
     2221 ttacggattc aggtttacga tatacgccaa gtgtgaaaat ggtgcgttca gaaaagatgg
     2281 ctagagccta tcttcaagga tgttttttgg caagtggcag tatcaattca cctaagtcga
     2341 gtaactatca tttagagatg tctagtcagg aaaaggattt ggcattgagt gttcaaaaat
     2401 tgatgcagcg tttttatttg cctgcaaaag taattgaaag aaaaagttta tatgtcgtat
     2461 atataaaggc tggagataaa attgctgatt tttt
//
LOCUS       DBMDWL010000242         2483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_19951_length_2483_cov_1.388329, whole genome shotgun sequence.
ACCESSION   DBMDWL010000242 DBMDWL010000000
VERSION     DBMDWL010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19951_length_2483_cov_1.388329"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="ACZPDN_11455"
     CDS             <1..137
                     /locus_tag="ACZPDN_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006507215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine aminopeptidase"
                     /protein_id="HIR1299269.1"
                     /translation="NQGTRHVHLNYNDDWTVYTDDGKLSAQWEHTFLVTEDGVEIISK
                     "
     gene            complement(163..504)
                     /locus_tag="ACZPDN_11460"
     CDS             complement(163..504)
                     /locus_tag="ACZPDN_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299270.1"
                     /translation="MSCHRIGLGMNSVVEKSIEMFENEEIGLNACKKIIVACRNGVYW
                     CDGNEDEAIACIIDCYCGNCLRKLHQEHRICVDRNRYDVVTHYLCEDCYQHLVYEESI
                     LKKHVYVEKTA"
     gene            complement(605..1285)
                     /locus_tag="ACZPDN_11465"
     CDS             complement(605..1285)
                     /locus_tag="ACZPDN_11465"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR1299271.1"
                     /translation="MEFIIDTVNLEDIKEAVEYMPIVGVTSNPSIVKKTSPKDFFKHM
                     KEVRKIIGKERSLHIQVISKECNEIVKEAHRILEEIDDQVYIKVPVSYEGIKAIKKLK
                     AEGVNVTATAVYDLMQAYMALAAGADYIAPYVNRIGNLGNDPMELINELSNRIVQDGY
                     DCKILAASFKGVQQVRDAFNYGSQAITAPVEVLKQIFANPSIEKAVNDFNSDWYDVYG
                     EGKGICDL"
     gene            complement(1297..>2483)
                     /locus_tag="ACZPDN_11470"
     CDS             complement(1297..>2483)
                     /locus_tag="ACZPDN_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HIR1299272.1"
                     /translation="NDEIIIPSFIEKGVKEEDAYNYSAIGCVETAVPGKWGYRCTGMS
                     YMNFPRILLMAMNDGVDMTSGKRFFEGSGYFKDMTSYDELFKAIEKGMRHLTRMSVIV
                     ENAIDLALERDVPDVLCSALTEDCIGRGKTIKEGGAVYDFISGLQVGIANMADSLAAI
                     KKLVFEERKITPEELWNAILDDYTSEENQRIQEMLINDAPKYGNDIDEVDQLVVDVYN
                     IYIDEMKKYPNTRYGRGPIGGIRYAGTSSISANVGQGYGTMATPDGRKAHTPLAEGCS
                     PAHAMDKKGPTAVFKSVSKLPTHEITGGVLLNQKVTPQLLSKEENKEKIEMLIKTFFN
                     RLKGYHVQYNVVSKETLLDAQAHPEKHKDLIVRVAGYSAFFNVLSKATQDDIIGRTEQ
                     TL"
BASE COUNT          755 a          506 c          318 g          904 t
ORIGIN      
        1 ttaatcaagg aacaagacat gttcacttaa attataatga tgattggaca gtttatacag
       61 atgatggaaa actttctgct caatgggaac atactttcct tgttacagaa gatggtgtgg
      121 aaattatttc aaaataaaaa gtcagaaaat ctgacttttt tattatgccg ttttttcaac
      181 atatacgtgc ttttttaata tactttcttc ataaacaaga tgttgataac aatcctcaca
      241 taaataatga gtcacaacat cataccgatt tctatcaaca caaattcgat gctcttgatg
      301 aagctttctt aaacaattac cacaataaca atcaataata caggcaatag cttcatcctc
      361 attcccatca caccaataaa caccatttct acaggcaaca ataattttct tacaagcatt
      421 taagcctatt tcttcatttt caaacatttc aattgatttt tcaacaactg aattcattcc
      481 tagaccaata cgatgacaac tcattttaac aacccctttc tagggttatt ttaacatctc
      541 atttttgtta tttcattaga cagatagtcc aaaaactgta gtcctcacga accacagttt
      601 tctcttataa atcacaaata cctttacctt ctccgtaaac atcataccaa tcactattga
      661 aatcattcac tgctttttca atacttggat tcgcaaagat ttgttttaag acttctactg
      721 gcgctgtgat tgcttgactt ccataattga aggcatctct tacttgttgt actcctttaa
      781 agcttgctgc taatatctta caatcataac catcttgtac gattctattt gataattcat
      841 tgatcagttc cattggatca tttcctaggt tccctattct attgacataa ggtgcaatat
      901 agtctgctcc tgctgccaag gccatatagg cttgcatcaa atcataaaca gctgttgctg
      961 tcacattgac tccttctgct tttagttttt tgatggcttt gattccttca tatgatacgg
     1021 gtactttgat atagacttga tcatcgattt cttctagtat acgatgggct tcttttacga
     1081 tttcattaca ttccttagaa atcacttgaa tatgaagact tctttctttt cctatgattt
     1141 tcctgacttc tttcatatgt ttaaagaaat ctttgggact tgtttttttc actattgaag
     1201 gattacttgt tactcctaca attggcatat attctactgc ttcttttata tcttctaagt
     1261 ttactgtatc aattataaat tccattttct tttctcctat aatgtttgtt cagttcttcc
     1321 aataatatca tcttgtgttg cttttgataa cacattaaag aatgcggaat agccagcaac
     1381 acgaacaatt aaatctttat gcttttcagg atgtgcttgg gcatctaata atgtttcttt
     1441 agaaacaaca ttgtattgaa catgataacc ttttaatcga ttgaagaagg ttttaatcaa
     1501 catttcaatc ttttctttat tttcttcttt cgataataac tgaggtgtta ccttttgatt
     1561 taataaaaca ccaccagtaa tttcatgtgt cggtaattta gaaacagatt taaacacagc
     1621 agttggtcct tttttatcca tcgcatgagc tggtgaacat ccctcagcaa gaggtgtatg
     1681 tgcttttctt ccatctggag tagccattgt gccataacct tgtccaacgt tggcagaaat
     1741 acttgaagta ccagcataac gaatcccacc gattggacct cttccataac gagtatttgg
     1801 atattttttc atttcatcaa tataaatatt ataaacatca actactaatt gatcgacttc
     1861 atcaatatca ttgccatact taggtgcatc attaattaac atttcttgaa ttctttgatt
     1921 ttcttcactt gtataatcat ctaaaatagc attccataat tcttcaggtg tgatttttct
     1981 ttcttcaaag acaagttttt taatagctgc aagactatct gccatattcg caattcctac
     2041 ttgtaaccct gaaataaaat cataaacagc gccaccttct ttgatggttt ttccacgacc
     2101 aatacaatct tctgtcaagg ctgaacataa aacatcagga acatctcttt caagtgctaa
     2161 atcaatcgca ttttcaacaa tcactgacat acgtgtcaaa tgacgcattc ctttttcaat
     2221 cgctttaaag agttcatcat aagatgtcat atctttaaag taaccactac cttcaaagaa
     2281 tcgttttcca gaagtcatat ctaccccatc attcattgcc attaataaaa tacgtgggaa
     2341 gttcatataa gacatccctg tacaacgata accccattta ccaggaactg ctgtttcaac
     2401 acaaccaatc gctgaatagt tatatgcatc ttcttctttg acaccctttt caataaatga
     2461 tggaataatg atttcgtcat tat
//
LOCUS       DBMDWL010000243         2476 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_20025_length_2476_cov_2.681263, whole genome shotgun sequence.
ACCESSION   DBMDWL010000243 DBMDWL010000000
VERSION     DBMDWL010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20025_length_2476_cov_2.681263"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            18..170
                     /locus_tag="ACZPDN_11475"
     CDS             18..170
                     /locus_tag="ACZPDN_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015935.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR1299273.1"
                     /translation="MIVTRKLQKCGQLLGIELLDHIIVGGTNGKMLSFREEKMLNVTG
                     KMDWER"
     gene            446..1687
                     /locus_tag="ACZPDN_11480"
     CDS             446..1687
                     /locus_tag="ACZPDN_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1299274.1"
                     /translation="MKTKIKGKEIKGLSSSVIYGPNAAGKTNIIGAMDVLRAIVLRGN
                     IRNSEEKSSPNPAVAALELIPNNNEMESKPVCFEIEFYEEDGEDHKFKIHYELEVDLG
                     TFLEEEHQRKILAEVLEVNGERVFERTQDLKIENLKIIKDYLSDITEQNAESVNEIAK
                     NSLNQEELFLTNGFKLIFSPKFTKLMVDWFTNKFMVIYRADSMQLIKRFADPKKKAIY
                     VEKTTDDAAKLFGINSNAVGYVVSDDEPDAKLYSVFKNMKNKKTTAVAADIFESYGTI
                     RFINMFPLVIKAMQTGGTLVVDEFDASIHPMALMSIINVFHNDDVNIHHAQLIFNTHN
                     PIFLNSNLFRRDEIKFVERDDDTHDSVLYALSDFGTTGDKGVRKHEDYMSKYFISQYG
                     AIKDIDFTPIFEEILCDEKEG"
     gene            1690..2355
                     /locus_tag="ACZPDN_11485"
     CDS             1690..2355
                     /locus_tag="ACZPDN_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HIR1299275.1"
                     /translation="MAKRKPTNKYYFSVEGETEQWYLKWLQDTINNTEKAACKVSIDC
                     PVRKNPLKHAKSLTVTRKIEIYHFFDYESDEPLHVKGFQEALDNMKKAEKIGKQIKYK
                     SGYSNFTFDLWIILHMTNCNASFSHRKQYITPINRAFGEKFQNMDEFKEENNFKRCLK
                     KMDLSNVIAAVDRARKIMQRNQENGYTLYQYKGYQYYKENPSLNAWEAIEKILKDCEL
                     IGK"
BASE COUNT          949 a          291 c          537 g          699 t
ORIGIN      
        1 aatcgtcaaa agaggatatg attgttacga gaaagctgca gaagtgcgga cagctactgg
       61 ggatagaatt gttggatcat atcattgtcg gtggaactaa tggaaagatg ctcagcttcc
      121 gagaagaaaa gatgttgaat gtgacaggaa agatggattg ggagagataa ggcaaatgag
      181 aaaaagtcag ggaatctatg atataaagat tccttgactt gatgaaaata aaatgatttt
      241 gaagaaataa tgcaaaatga gaataaaaaa ttgactctgt catacaataa gactataata
      301 taaatatgat tttgttaaaa ataagcagaa tggaggtaat aaaatgcttc ttgaatttaa
      361 aacaaaaaat tataaatctt ttgtagaaga ggcaagcttt tcaatggtag ctgcgccaaa
      421 acaaaaggga ttagattata gtcttatgaa gacaaaaatt aaaggaaaag agataaaagg
      481 gttaagttct tcagttattt atggaccgaa tgcggcagga aaaactaata ttattggtgc
      541 gatggatgta ttgcgggcaa ttgttttgag aggaaatatc agaaattctg aagaaaaatc
      601 atcaccgaac ccagctgttg cagcattaga attgattcca aataataatg aaatggaatc
      661 aaaaccggta tgttttgaaa tagaatttta tgaggaagat ggagaagatc ataaatttaa
      721 aatacactat gaattagagg ttgatcttgg aacctttctt gaggaggagc atcaaagaaa
      781 aatccttgca gaagtgctgg aggtcaatgg agaaagagta tttgaacgta cgcaagattt
      841 gaaaatagag aacttaaaga ttattaaaga ttatttgtcg gatataacag aacagaatgc
      901 agaaagtgta aatgaaattg caaaaaatag tttgaatcag gaagaattat ttttgacgaa
      961 cgggtttaaa ttgatttttt cgcctaaatt cacaaagctg atggttgatt ggtttacaaa
     1021 taagtttatg gtaatttatc gtgcggattc gatgcaattg attaaaagat ttgcagatcc
     1081 aaaaaagaaa gcaatctatg ttgaaaaaac gacggatgac gctgcaaaat tatttggaat
     1141 caattctaat gcagtaggat atgttgtgag cgatgatgag ccggatgcaa aattatattc
     1201 ggtatttaaa aatatgaaaa acaaaaagac tacggcggtt gcagcagata tttttgagtc
     1261 atatggaacg attcgattta tcaatatgtt tcctcttgtg attaaagcga tgcaaacggg
     1321 tggaacactg gtggtggacg aatttgatgc gtctattcat ccgatggcat tgatgagtat
     1381 tatcaatgta tttcacaatg atgatgtaaa catccatcat gcacagctta tatttaatac
     1441 gcataatccg atatttttga attcaaatct tttcagaaga gacgagatta agtttgttga
     1501 gcgtgatgat gatacacatg acagcgtatt atacgcatta tctgattttg gcacaaccgg
     1561 tgataagggt gttcgtaagc acgaagatta tatgagcaaa tatttcatca gccagtatgg
     1621 agcgataaag gatattgatt ttacaccaat ctttgaagaa atcttatgtg atgaaaaaga
     1681 gggataggga tggcaaagag aaaaccaaca aataaatatt atttcagtgt tgagggagaa
     1741 acggagcagt ggtacttaaa atggctgcag gatacgataa ataatactga aaaagctgca
     1801 tgcaaggtgt ctattgattg tccggtaaga aaaaatccat taaagcatgc gaaatcattg
     1861 accgttacca gaaagattga aatctatcat ttttttgatt acgaaagtga tgaaccgctt
     1921 catgtcaaag gctttcaaga agctctggac aatatgaaga aggcagaaaa aataggaaaa
     1981 cagataaaat ataaatctgg atatagtaat tttacttttg atttatggat cattcttcat
     2041 atgacgaact gtaatgcgtc attttcacat cgaaaacagt atattacacc gattaaccgg
     2101 gcgtttggag agaaatttca aaatatggac gaattcaaag aagagaataa tttcaagaga
     2161 tgtcttaaga aaatggactt gtccaatgta attgcggctg ttgatcgggc aaggaaaatc
     2221 atgcagagaa atcaagaaaa tggatatact ttgtatcaat ataaagggta tcagtattat
     2281 aaagagaatc cttcattgaa tgcatgggag gctattgaga agatattgaa ggattgtgaa
     2341 ttaataggaa agtaaaagat acatgctgtt actaaaaata aaaatgcaat gggagtatat
     2401 atgtataaat ttagaataga taaattagag ctaaatacta tagatggagt aatagatttt
     2461 gaacctagac ggatca
//
LOCUS       DBMDWL010000244         2474 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_20053_length_2474_cov_1.753477, whole genome shotgun sequence.
ACCESSION   DBMDWL010000244 DBMDWL010000000
VERSION     DBMDWL010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20053_length_2474_cov_1.753477"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(57..>321)
                     /locus_tag="ACZPDN_11490"
     CDS             complement(57..>321)
                     /locus_tag="ACZPDN_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299276.1"
                     /translation="TEKVEEPVKEETTKKEEKSSTSFDESQQQAINSALANSGLEQSD
                     VSDITCSLDSNTNQYTVTFVLNGVTTTAVVDAATFTVISTILG"
     assembly_gap    322..331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<332..609)
                     /locus_tag="ACZPDN_11495"
     CDS             complement(<332..609)
                     /locus_tag="ACZPDN_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299277.1"
                     /translation="MMVKRNSLIFLTAFGLLLTGCSKSGFEITKDDILEVARDDANAT
                     KSECENVSIKEQKGSYIVSFNTNGGSYEYKIGKDGIIKERSFKRGAKE"
     gene            complement(590..1300)
                     /locus_tag="ACZPDN_11500"
     CDS             complement(590..1300)
                     /locus_tag="ACZPDN_11500"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR1299278.1"
                     /translation="MAKKKRNQFDVDEIYRGLRTNIEFSQMDEAMQVITCVSTVPNEG
                     KSTIACNLARIMAAKYKNVLLIDCDLRNASVHKTLHISNRSGLTNIISEFQEGLSINS
                     YEGVQQVQYDGGQTLTVITAGHRVPNPSEVLGSKRMGRFLEQARKEFGYIIIDSPPMA
                     VASDTIPLSNVSDGVLYVVDAKSSDKRRVKTAINDLKRNGGHVIGVVLNKVDMSDENH
                     YGYGYGYGYGGDDDGKKK"
     gene            complement(1303..2079)
                     /locus_tag="ACZPDN_11505"
     CDS             complement(1303..2079)
                     /locus_tag="ACZPDN_11505"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR1299279.1"
                     /translation="MAKFIDIHSHIAWGIDDGMPSIEDAQSALESARADGFVGICSTP
                     HFIPGQLDVSVYNEIVSRQMELKEMSPIPIYCGGEVMMNSEFIDGLDLDLYPTLNGSR
                     YMLVEYNVLRDIHSIDYRDDCLYELKVKGFVPVIAHIERYFHSGLDYSIIDNWIDMGC
                     VLQINRTSILGMHGKTIQSNALSLLDDGYCDVIGTDTHRASGHRISKLSDAYSIVSKR
                     IGSENADILFFENPKRILSDMDILNIEVVKKKKRFSFFRR"
     gene            complement(2079..>2474)
                     /locus_tag="ACZPDN_11510"
     CDS             complement(2079..>2474)
                     /locus_tag="ACZPDN_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR1299280.1"
                     /translation="VAKDVGMENAASVKKCITVTNEANTEIITITATTTDPKLSKNIA
                     NGTVSTFTKTMQKNLNVRNIEVVDKAKLSYVPSGPNIKKNTMLATLVGGVIGVGYAVL
                     RFLFDNRLRTKEEAEKYLGIPVFCEIPEL"
BASE COUNT          815 a          540 c          296 g          813 t
ORIGIN      
        1 taatataata gcatacacat aaaaaaaata cctcccattt ctggaaggta gtatgcttaa
       61 cctaaaattg tagatataac agtaaatgta gctgcgtcta caactgctgt tgtagtaaca
      121 ccattcaata caaatgtgac tgtatattgg tttgtgtttg aatctaaaga acaagtaata
      181 tcactaacat cactttgttc taagccagaa ttagccaaag cagaattaat tgcctgttgt
      241 tgagattcat caaatgaagt tgaagacttt tcttcttttt ttgtagtttc ttctttaact
      301 ggttcttcaa ccttttccgt tnnnnnnnnn ntcttcttta gctccacgct taaaactacg
      361 ttcttttata atcccatctt taccaatctt gtattcgtaa gatccaccat ttgtattaaa
      421 tgaaacaata tacgaacctt tttgttcttt gattgataca ttttcacatt cacttttcgt
      481 ggcattcgca tcatctctag ctacttctaa aatatcatct tttgtaattt caaagcctga
      541 cttactacag cctgttagaa gtagaccaaa agctgtaagg aatattaaac tatttctttt
      601 taccatcatc gtctcctcca taaccatagc catatccata gccataatgg ttttcatcac
      661 tcatatctac tttgtttaat acaacaccaa taacgtgtcc accattacgt tttaagtcat
      721 tgatagctgt tttaactcta cgtttatctg aagatttcgc atccactaca tataatacgc
      781 catcactcac attactcaat ggaatcgtat cactagctac agccattggc ggtgagtcta
      841 taataatata tccaaattct ttacgagctt gttctaaaaa gcgtcccatt cttttagatc
      901 ccaatacttc actaggatta ggtactctat gacctgctgt aataacagtc aatgtttgac
      961 caccatcata ttgaacttgt tgaacaccct cataagaatt aatactaagt ccttcttgaa
     1021 attcggaaat aatatttgta agtccagaac gatttgaaat atgcaacgtt ttatgaaccg
     1081 atgcatttct taaatcacaa tcaatcaata atacattttt gtattttgct gccataatac
     1141 gagccaagtt acaagcaatc gtactctttc cttcatttgg aactgtactt acacaagtaa
     1201 taacctgcat agcctcatcc atttgactaa actcaatatt tgtacgcaac ccacgataaa
     1261 tctcatctac atcaaactga tttctctttt tctttgccat ttctatctcc taaagaaact
     1321 aaaacgcttc tttttcttta caacctcaat atttaatata tccatatcac tcaagatacg
     1381 cttaggattc tcaaaaaata atatatccgc attctcacta ccaatacgct ttgaaacaat
     1441 tgaatacgca tcactcaatt tagaaatacg atgcccactt gctctatgtg tatctgtacc
     1501 aatcacatca caatatccat catctaataa agataatgca ttcgattgaa ttgtctttcc
     1561 atgcattcct aaaatagaag tacgattgat ttgtagaaca caacccatat caatccaatt
     1621 atcaataatc gaataatcaa gtccggaatg aaaataacgt tcaatatgcg caatcacagg
     1681 aacaaaaccc tttaccttca attcatacaa acaatcatct ctataatcaa tggaatgaat
     1741 gtcacgaaga acattatact ctacaagcat ataacgagat ccatttagtg taggatataa
     1801 atctaaatcc aacccatcaa taaactcact attcatcatg acttcaccac cacaataaat
     1861 tggaatcgga ctcatttctt ttaattccat ttgccgagaa acaatctcat tataaacaga
     1921 aacatctaat tgtccaggaa taaagtgtgg tgtactacat attcctacaa agccatcagc
     1981 cctagcagat tctaacgcac tttgagcatc ctcaatcgat ggcatgccat catcaatacc
     2041 ccaagcgata tgtgagtgaa tatctataaa cttagccatt ataattcagg aatctcgcag
     2101 aaaacaggaa tacccaaata tttttctgcc tcttctttcg tacgcaaacg attatcaaac
     2161 aagaatctta atactgcata tcctacacca atgacaccac caactaaagt agccaacatt
     2221 gtgtttttct taatattagg tccactaggt acataagaca atttagcctt atcaacaacc
     2281 tcaatattac gaacattcaa attcttctgc atagttttag taaaagtact tactgtacca
     2341 tttgcgatat tcttagataa cttaggatct gtagttgtag ccgtaatcgt aataatttca
     2401 gtattcgctt cattcgttac tgtaatacac tttttaacgc ttgccgcatt ttccatacca
     2461 acatccttag ctac
//
LOCUS       DBMDWL010000245         2473 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_20068_length_2473_cov_2.596121, whole genome shotgun sequence.
ACCESSION   DBMDWL010000245 DBMDWL010000000
VERSION     DBMDWL010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20068_length_2473_cov_2.596121"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="ACZPDN_11515"
     CDS             <1..624
                     /locus_tag="ACZPDN_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR1299281.1"
                     /translation="GYQTMPDVIGQSQEDAIDTLQDAHFNTSKVTYVYRANDKYEKGK
                     IIKSSHKKGEVILNDTKIVLTVSKGATYLVPDFTDGTYVDAEYELEKNCPNVNIVVEY
                     KGKKDLDPGIVLKQKGLTPGTRMDPDSKKTITFVVSTYPSIVIPSDLIGKDVLDAKDE
                     LNDLGIAAVLSKISDGSGSNKVISVSPDVGTEYVQEGTNSVVTLYYD"
     gene            578..1480
                     /gene="rsgA"
                     /locus_tag="ACZPDN_11520"
     CDS             578..1480
                     /gene="rsgA"
                     /locus_tag="ACZPDN_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1299282.1"
                     /translation="MYRKEQTVLLHCIMINMQQGRIIKIISNQYTIQNEKEEIIAKPR
                     GKMRQQQTPVVGDFVEYEKIEDSYRIHKVLPRTNRLLRPAIANVDQALIVTSLKDPDY
                     SCHLLNRLIFLVGLAGVKPVICVTKLDLLEDPEEILADLEKYKKAGYDVVFSNPGSDD
                     RELQEILNGKVSVLCGQSGAGKSSLLNRLNPDFELQTQAISKALGRGKHTTRYCQLHP
                     VCDGLVADTPGFSSLDFTRLDTSHLDACILDFKPYIHTCRFKDCKHLNEPDCSIKEAV
                     NKGEINASIYEDYKNIMEERGKKL"
     gene            1477..2136
                     /gene="rpe"
                     /locus_tag="ACZPDN_11525"
     CDS             1477..2136
                     /gene="rpe"
                     /locus_tag="ACZPDN_11525"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1299283.1"
                     /translation="MNEIVIAPSILSLDYSNVSAQLEELKASKAKWLHFDVMDGHFVP
                     NLTFGPDILKGFKKACPLFMDVHLMVEDPKTYAPIFIQNGADLVTFHTEALDNDLSRI
                     QGLLDEIHNLGAKGGIVVKPNTAIEPFESVLKSCDLVLVMSVEPGFGGQKFMADMLKK
                     VEWLKEKREELNLSYRIEIDGGINQDTYKSALDAGCDTLVAGSYVFKNDITKTIEGML
                     A"
     gene            2139..>2473
                     /locus_tag="ACZPDN_11530"
     CDS             2139..>2473
                     /locus_tag="ACZPDN_11530"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR1299284.1"
                     /translation="MTCAVLITPLVDVVPIIEGCDYIGVDAGALKIIDQGLPIKMAVG
                     DFDSLNEEDLKRITCPIERHPIMKDETDSELAIRLCKDYDTIYLYGGIQGRIDHTIAN
                     IRLAMYRFN"
BASE COUNT          839 a          335 c          541 g          758 t
ORIGIN      
        1 ggctatcaaa cgatgccaga tgtgattgga caaagtcaag aagatgcgat cgatacatta
       61 caggatgcgc attttaatac ttccaaagtc acatatgtct atagggcaaa tgataaatat
      121 gaaaagggaa aaatcattaa gtcttcccat aaaaagggtg aagttatttt aaatgataca
      181 aagatcgttt taactgtatc taaaggtgca acctatttag tacctgattt tacagatgga
      241 acgtatgtgg atgctgaata tgaattggaa aagaattgtc cgaatgtaaa tattgtcgtg
      301 gaatacaagg gtaagaaaga tttggatccg ggtattgtgt tgaaacaaaa gggcttaact
      361 ccaggaactc gaatggatcc ggacagtaaa aaaacaataa cattcgtcgt aagtacatat
      421 ccttctattg tgattccgtc ggatttgatt ggaaaagatg tattagatgc caaagatgaa
      481 ttgaatgatc taggcattgc ggctgtttta agtaaaattt cagatggttc aggaagtaat
      541 aaagtaatta gtgtctcacc agatgtaggg acagaatatg tacaggaagg aacaaacagt
      601 gttgttacac tgtattatga ttaatatgca gcagggaaga attataaaaa ttatttcgaa
      661 tcaatatacg attcaaaatg aaaaagaaga aattattgcc aaaccaagag gtaaaatgcg
      721 ccagcaacaa acgcctgttg taggggattt tgtagaatat gaaaagattg aagattcgta
      781 tcgcattcat aaagtgttgc cacgaacaaa tcgtctttta agaccggcca ttgccaatgt
      841 cgatcaggca ttgattgtca caagtttaaa agatccagat tattcatgtc atcttttaaa
      901 tcgtttgatt ttcttggtag gtctggctgg agtaaaaccg gtgatttgtg tgacaaaact
      961 ggatttgtta gaggatcctg aagaaatctt ggcggattta gaaaaataca aaaaggctgg
     1021 atatgatgtg gtattctcaa atccaggtag tgatgataga gaattgcagg aaattttaaa
     1081 tggtaaagta tccgtattat gtggtcaatc tggagcgggt aagagtagtt tattgaatcg
     1141 cttaaatccg gattttgaat tgcagacaca agctatctca aaagctttag gacgtggtaa
     1201 acatacaaca cgctattgcc aattacatcc agtatgtgat ggattagtgg cggatacgcc
     1261 aggtttttca agcttagatt ttactagatt agatacatcc catttagatg cgtgtattct
     1321 tgattttaaa ccttatattc atacgtgtcg atttaaagac tgcaagcatt taaatgaacc
     1381 ggattgtagc attaaagaag cggtcaataa aggtgaaatc aacgcgtcaa tctatgagga
     1441 ttataaaaat ataatggaag aaagaggtaa gaaactatga atgaaattgt aattgcacct
     1501 tcaattttat ctttggatta ttccaatgtg agtgcgcaac ttgaagagtt aaaggcatcc
     1561 aaggcgaaat ggttacactt tgatgtgatg gatggtcatt ttgtaccaaa cttaactttt
     1621 ggacctgata ttttaaaagg ctttaaaaag gcttgtccat tatttatgga tgtgcatttg
     1681 atggttgaag atcccaaaac gtatgcacca attttcattc aaaatggagc ggatttagtc
     1741 acattccata cagaagcttt agataatgat ttaagtcgta ttcaaggctt attggatgaa
     1801 attcataatt tgggggcaaa aggcggtatt gtggttaagc caaatacagc gatcgaacct
     1861 tttgaaagtg tattaaaatc ttgtgattta gttttagtta tgagtgtaga gcctggcttt
     1921 ggtggtcaaa aattcatggc agacatgctt aaaaaggtag agtggttaaa agagaaacgt
     1981 gaagaattga atctgtctta tcgcattgaa atagatggtg gtattaatca agatacatat
     2041 aaatctgcat tggatgcagg ttgtgataca ttggttgcgg gaagctacgt atttaaaaat
     2101 gatattacta aaaccattga aggtatgctt gcataaatat gacttgtgct gttttgatta
     2161 ccccactcgt tgatgttgta cctattatag aaggttgtga ttatattggc gtagatgcgg
     2221 gggcattaaa aattatagat caaggtcttc ctattaaaat ggctgttgga gactttgatt
     2281 ctttaaacga agaagattta aaacgaatta cgtgtccgat tgaaagacat ccaatcatga
     2341 aagatgaaac ggattcagaa cttgcgattc gattatgtaa agattatgat acgatctatt
     2401 tgtatggtgg aatacagggc agaatcgatc atacaattgc caatattcgt ttggctatgt
     2461 atcgttttaa taa
//
LOCUS       DBMDWL010000246         2473 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_20070_length_2473_cov_2.147555, whole genome shotgun sequence.
ACCESSION   DBMDWL010000246 DBMDWL010000000
VERSION     DBMDWL010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20070_length_2473_cov_2.147555"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="ACZPDN_11535"
     CDS             <1..497
                     /locus_tag="ACZPDN_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1299285.1"
                     /translation="GSSSVNELMKSALKDVDGDLQFLYVCGKDNSQDLNLFENQKNIH
                     VVPYVDTLRIYGHVDGMVCRAGATTLAEVTALGIPSIVIPSPYVANNHQFYNASMLLK
                     KQACRIIEEKDLNAETLQGQIVSLYANPVVYKETHEHALQMGKPNAAYDIIDLLKNIC
                     KKGK"
     gene            499..1398
                     /gene="murB"
                     /locus_tag="ACZPDN_11540"
     CDS             499..1398
                     /gene="murB"
                     /locus_tag="ACZPDN_11540"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1299286.1"
                     /translation="MSIKDRLMTYAEVLEHESMKKHTTFKIGGFVDYYIYPKNCTALM
                     CVLDILAEENIPYYVLGRGSNVLCSDTDFHGAIINLDRTLNDFYFEPDGILVAQAGCS
                     IINMAVEAMKRSLTGLEWASGIPGSIGGCLYMNAGAYKDNMANHLVDVCVLKDNTICW
                     MKKDELDYAYRHSIFQSHKDWTILVGRFQLEKGNQNDIRDLMDARRERRMSAQPLNKP
                     CAGSVFRNPEEIPAWKLIEDLGLRGHKVGGAKVSEKHCNFIVNDDLTATAQDVRDLIH
                     EIKVKAKKEYDIDMVTEVEQLYW"
     gene            1392..2390
                     /locus_tag="ACZPDN_11545"
     CDS             1392..2390
                     /locus_tag="ACZPDN_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118320522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OCRE domain-containing protein"
                     /protein_id="HIR1299287.1"
                     /translation="MVKKKIKLTIKQTIIFSFVFMMVASVFVYYFSTDSHVKVLSCKN
                     NYYLSDNEVYDLANVSTKTRLYFVPSILFEKRVEKMPFVQSCKVSKKNRKLTFDVQEK
                     LIVGYYVKGNKNFALFQDGTSIEIEEQYLNMIVHFPLLSDFNAKQRKQLCEQFQKHSK
                     VLTRDLIEKFAEIVPYKASYDKNMFKITMQDGNIVYTNLNSIKMLSKYQSVLTKLKGQ
                     SVCLVLDSTHSTIEKVNCEDLNSKKKEEEQKKTSEKAETETSQETENQEKQQPEDEET
                     ENEAEWVYDENTGVYYHEAIGMYYDPNTDEYYDENGTYYYWDEDSQSFVEAYSSYA"
BASE COUNT          895 a          289 c          512 g          777 t
ORIGIN      
        1 taggttctag ttcagtaaat gaattgatga agagtgctct aaaggatgtt gatggtgatt
       61 tacaattttt gtatgtctgc ggaaaagata attcgcaaga tttgaatttg tttgaaaacc
      121 aaaaaaatat tcatgttgta ccatatgtag atactttacg tatatatgga catgtagatg
      181 gaatggtttg tcgtgcagga gcaactacac ttgcagaagt aacagcttta ggtattccat
      241 ctattgtgat accaagtcct tatgtggcaa acaaccatca attttataat gcgagcatgc
      301 tattaaaaaa acaagcttgt cgtattattg aagaaaaaga tttaaatgca gaaacattgc
      361 agggacaaat tgtttcttta tacgcaaatc ctgtggtata taaagaaacg catgagcatg
      421 ctttgcagat gggaaaacca aatgcggcat atgatattat agatttatta aaaaatatat
      481 gtaaaaaggg gaagtaatat gagcattaag gatcgtttaa tgacgtatgc agaagtttta
      541 gaacatgaat ctatgaagaa acatacaact tttaagattg gtggctttgt agattattat
      601 atttatccaa aaaattgtac agctttgatg tgtgtactcg atatacttgc agaagaaaat
      661 attccatatt atgtacttgg acgtggatca aatgttttat gttcagatac ggattttcat
      721 ggtgcaatta taaatttaga tcgtactctg aatgactttt attttgaacc agatggaatt
      781 ttagtggcac aagcaggatg ttcaattata aatatggcgg tagaagccat gaaaagatcg
      841 ttgacaggat tggaatgggc aagtggtatt ccaggctcta ttgggggatg tctgtatatg
      901 aatgcaggtg cctataaaga taatatggca aatcatttag tggatgtatg tgttttaaaa
      961 gataatacaa tttgttggat gaagaaggat gaattggatt atgcatatcg ccattctatt
     1021 tttcaatcac ataaagattg gactatttta gtgggtcgtt tccaattaga aaaaggaaat
     1081 caaaatgata ttcgtgattt aatggatgct agacgcgaaa gaagaatgag cgcacaacca
     1141 ttgaataagc catgtgcagg aagtgttttt agaaatccag aagaaattcc tgcttggaaa
     1201 ttgattgaag atttaggttt acgtggccat aaagttggtg gagctaaggt gagtgaaaag
     1261 cattgtaatt ttattgtgaa tgatgattta accgctacag ctcaagatgt tcgtgattta
     1321 attcatgaaa tcaaagtcaa ggctaaaaaa gaatatgata ttgacatggt aacggaagtt
     1381 gagcagttat attggtaaag aagaaaataa agctaaccat caaacaaaca attattttta
     1441 gttttgtttt tatgatggtg gctagtgttt ttgtgtatta cttttctaca gattcccatg
     1501 tcaaggtttt gtcgtgtaaa aataattact atttatcaga taacgaagtt tatgatttgg
     1561 caaacgtgag tacaaagact cgtctttatt ttgtaccttc tattctattt gaaaaaagag
     1621 tagagaagat gccatttgta cagtcttgta aagtatcaaa gaagaatcgt aaattgactt
     1681 ttgatgtaca agaaaaattg attgttggct attatgtaaa gggtaataaa aattttgcac
     1741 tgtttcagga tggaacttca attgaaattg aagaacaata tttgaatatg attgtgcatt
     1801 ttcctttatt gtccgatttt aatgcgaaac aacgtaaaca gttatgcgaa caatttcaaa
     1861 aacatagcaa agtattaaca cgggatttaa tagaaaagtt tgcggaaatt gtaccttata
     1921 aagcgagtta cgataaaaat atgttcaaga tcacgatgca ggatggtaat attgtttata
     1981 cgaatttaaa tagtataaaa atgctttcaa aataccaaag tgtattgacg aaacttaagg
     2041 gacagtcggt atgtttggtt ttagattcta cacattctac gattgaaaaa gtaaattgtg
     2101 aggatttgaa ttctaagaaa aaggaagaag agcaaaaaaa gacttctgaa aaagcggaaa
     2161 cagaaacttc acaagaaact gaaaatcaag aaaaacagca accggaagat gaagaaaccg
     2221 aaaatgaggc ggagtgggtt tatgatgaga atacgggagt atattatcat gaagctatag
     2281 gtatgtatta tgatccgaat accgatgaat attatgatga aaatgggaca tattattatt
     2341 gggatgagga ttcacaaagc tttgtagaag cgtatagtag ttacgcatga aaaataaaat
     2401 ggcttaaata aacgaaatcg gaaactctat taaagaattt ccgatttttt ctagtgttca
     2461 gatggcaata tta
//
LOCUS       DBMDWL010000247         2441 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_20443_length_2441_cov_1.702137, whole genome shotgun sequence.
ACCESSION   DBMDWL010000247 DBMDWL010000000
VERSION     DBMDWL010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20443_length_2441_cov_1.702137"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(250..2031)
                     /locus_tag="ACZPDN_11550"
     CDS             complement(250..2031)
                     /locus_tag="ACZPDN_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1299288.1"
                     /translation="MPKKNVKNGKKSNRVISKFLTIFMIAALALLIFQIIDLNLLPMK
                     LMILVSLVLVILTLIIILMLHFKARKFFSRLLMGLVALCMCAGLAFGNYFIFKTDNTF
                     EKVTSLADSKATSTSIVVLKASSIEKEKDLAGAKIGTILDMDEEATSRMLKDLDSDKI
                     KYTTKDYSSLDDMMEAFYNSEVDAICLNEKYRDILHEGGAYFNFQTDSRVVHQNVYYT
                     KVTKTDNPSDPVRNITRDTFTVLVSGNDSYGTLQDSNTRSDANLLLTVNPKTGTILMT
                     SIPRDYYVELVCPEGSYDKLTHSGLMGVKSTEKTIEKALGITINYNIRINFSSVVSLV
                     DALDGIDLDIKKGEEVDIFYTNSQPGLSVGKHHVDGETALAFARERHAYADGDNQRVR
                     NQQKVFKAMFKRIVSPKMITNYGKFMDALAVAFDTNLSGDEISKFVKYELNRMPKWKI
                     ESYAIVADSDTKFCYQAQSYASVVVQNDIMNEVARKKIQAVIDGKSSETVEDPSGFSQ
                     TPNENNAIGNTAELEAMGIINNSGSSSNNSSNDYDSNYDNNYDYSYDNSNDYNYSNNY
                     DSSYDDSNYNNPDNNYYEDDSYDDTAG"
     gene            complement(2147..>2441)
                     /locus_tag="ACZPDN_11555"
     CDS             complement(2147..>2441)
                     /locus_tag="ACZPDN_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299289.1"
                     /translation="DDVMRPVWGDDYAVCCFIGVIILNVVYIIVPVIWLVLASIVCTL
                     GGLLLFFFDSLNGYLEKSLSYKHKAESLGYEKSLYKAKENGFKGFAYRCERYM"
BASE COUNT          842 a          506 c          286 g          807 t
ORIGIN      
        1 ttctaaacgg tttaatttca taaattttca ttatgcgaat catctttatt ggtacttaag
       61 ctcaaaatca cttcgtcttt aaaaggtaaa aagctagtta ccgaaaattg tattcacatt
      121 ttagcacctt ctaacttaca taataaatag aaatctcaac ttattttctt tattttagat
      181 attacaatgc attttactaa tacaatgcat tttacgcatt aaaaaaagat acccgaaggt
      241 atcttagtgt taaccagccg tatcatcata actatcatct tcataatagt tattatctgg
      301 attgttgtag tttgaatcat catagcttga atcataatta ttactataat tataatcatt
      361 gctgttatca taactataat cataattgtt gtcgtagtta gaatcgtaat cattactact
      421 attatttgat gaagatccac tattattaat gattcccatc gcttcaagtt ctgctgtatt
      481 accaatcgca ttattttcat ttggcgtttg cgagaatcca gaaggatctt ctaccgtttc
      541 tgaactcttt ccatcaatga cagcttgaat cttctttcta gccacttcat tcataatatc
      601 attttgaacg acaacactcg catagctttg tgcttgataa cagaattttg tatctgaatc
      661 tgcaacaatc gcatacgatt caatcttcca tttaggcatt ctatttagtt catacttcac
      721 aaacttacta atttcatcac ccgataggtt tgtatcaaac gcaacagcta aggcatccat
      781 aaacttacca tagtttgtga tcatcttagg agaaacaata cgtttaaaca tagctttaaa
      841 tactttttgt tgattacgaa cacgttgatt gtcaccatct gcataagcgt gtctttcacg
      901 tgcaaaggct aaggctgttt caccatccac atgatgttta ccaacactta agccaggctg
      961 cgaattcgta tagaaaatat cgacttcctc acctttttta atatccaaat caattccatc
     1021 taaggcatcc actaaactta caacactcga aaagttaatg cgaatattat aattaattgt
     1081 gattcccaaa gctttttcaa ttgtcttttc cgtactcttc acacccatta atccagaatg
     1141 tgttaatttg tcataactac cttcaggaca aacaagttct acataataat cacgaggaat
     1201 acttgtcatt aaaatcgttc ctgtttttgg attgactgtt aataataagt tggcatcact
     1261 acgcgtattc gaatcttgta acgtaccata tgaatcattt ccacttacta atacagtaaa
     1321 cgtatctcta gtaatatttc ttactggatc acttggatta tcagtcttag tgacttttgt
     1381 ataatacaca ttttgatgca cgacacgaga atccgtttgg aaattaaaat acgctccacc
     1441 ctcatgtaag atatcacgat acttttcatt taaacaaatc gcatccactt cactattata
     1501 aaaagcctcc atcatatcat ccaatgaaga ataatccttc gtcgtatatt taatcttgtc
     1561 tgaatccaaa tccttcaaca tacgacttgt tgcctcttca tccatatcta ggatcgtacc
     1621 aatcttagca cctgcaagat ccttctcttt ttcaatcgaa gaagccttta aaacgacaat
     1681 cgaagttgaa gtggccttac tatccgcaag acttgtcacc ttttcaaaag tattatccgt
     1741 tttaaaaata aaataattgc caaaagctaa acctgcacac atacatagtg caacaagtcc
     1801 catcaaaagt cttgaaaaaa acttacgagc tttaaaatgc aacattaata taataatcaa
     1861 agtcaatata actaagacta aagacactaa gatcatgagt ttcattggta ataaattcaa
     1921 atcaatgatt tgaaaaatca ataatgccaa agccgcaatc ataaatattg tcaaaaattt
     1981 actgattacc ctatttgatt ttttaccatt ctttacattt tttttaggca taaaaataat
     2041 ctcctataaa cttctatatg gctcacaatt atatattata gtataaatcc aaccattttg
     2101 gcaaatcagc caaaaaaagg gatagtcaat atgaccatcc cacaatctac atatatcttt
     2161 cacaacgata cgcaaaacct ttaaaaccat tctctttcgc cttatacaaa ctcttttcat
     2221 aacccaaaga ttcagcctta tgtttataag acaatgactt ctctaagtac ccattcaaac
     2281 tatcaaagaa gaatagcaat aaaccaccta aagtacatac aatacttgct agaacaagcc
     2341 aaataacagg tacaatgata taaactacat ttagtataat cacaccaata aagcaacaaa
     2401 ccgcataatc atcaccccat acaggacgca tcacatcatc g
//
LOCUS       DBMDWL010000248         2403 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_20888_length_2403_cov_3.550825, whole genome shotgun sequence.
ACCESSION   DBMDWL010000248 DBMDWL010000000
VERSION     DBMDWL010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20888_length_2403_cov_3.550825"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            169..528
                     /locus_tag="ACZPDN_11560"
     CDS             169..528
                     /locus_tag="ACZPDN_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1299290.1"
                     /translation="MPINKSTEYGNIEISLDAIANLAGGAITECYGIVGMASQKTLKD
                     GWAELLKKENYARGVVVTQDETGLVLNLYIIALQGIKLSEVVYEAQKRVKYVLEKTLE
                     VKCNAVNIYVQGVRAVK"
     gene            539..2164
                     /locus_tag="ACZPDN_11565"
     CDS             539..2164
                     /locus_tag="ACZPDN_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR1299291.1"
                     /translation="MEKINGVLFKQMLESGMNNLSNHSAEIDALNVFPVPDGDTGTNM
                     HLTVSNGVKEALKTNSDSVKDIAKTLSRGLLMGARGNSGVITSQIFRGIYQAVEDLDE
                     MDASQLAHALVNGSRVAYRAVMRPVEGTILTVVREAADYTYAYTTTEEVTDCMDVMKK
                     MVDEANASLQRTPELLPVLAEVGVVDSGGKGLCVILEGFLAAMEGHPVQLEGAKAAGE
                     SAQAKVEGGEEEYGFCTEFILKLSENGIRHFSETSFKDELATIGNSIVCVQDEDLVKV
                     HVHTLEPFTAIKMGRRQGRFIKLKVENMQEQHDNIIEKEEEEKMAEHKKYAIITVAPG
                     DGIKKMFTELRADIVVGGGQTMNPSTEDFVSAVEKLNADHIFILPNNSNIVLAASQAA
                     QVCSNQDIHVLPSKTIPQGLSACVMFNPDVELEDNLAEMNEAIENVKSGEVTYAIKDT
                     TYDGLEIKKDEYMGIFGKDICVSCPDCMEATKKLLDKMLDDDSELVTLIYGDTATEEQ
                     AEEIAAYIEDNSDAEVEIHEGNQPVYSFIIGVE"
BASE COUNT          836 a          309 c          508 g          750 t
ORIGIN      
        1 ttcataataa tagtgtacac caatacacta ttaaagtata taaaaaaata aataatcttc
       61 aaacatgaaa aattgaagat tatttcatgc gtaatcacta agaagcgtat taggatttgt
      121 gtttatattt taagtagagt ataattaata aaaacaggag gttttattat gcctattaat
      181 aaatcaacag aatacggaaa tatcgaaatt tcattagatg cgattgccaa tttagcaggt
      241 ggtgcaatta ctgaatgtta cggtattgta ggtatggcaa gccagaaaac attgaaggat
      301 ggctgggcag aattattgaa aaaggaaaat tacgctcgtg gtgttgttgt gacacaagat
      361 gaaacgggtt tggttttaaa tctttatatt atcgcattgc aaggaattaa attaagcgaa
      421 gttgtatatg aagcacaaaa gcgcgttaaa tatgtattgg aaaagacttt ggaagttaaa
      481 tgtaacgctg taaacattta tgttcaaggt gtacgtgcag ttaagtaggg gttaaaaaat
      541 ggaaaaaatc aatggtgtat tatttaaaca gatgctagaa tccggcatga ataatttatc
      601 aaatcattca gccgaaattg atgcattgaa tgtttttcct gtaccggatg gtgatactgg
      661 aacaaatatg catttaactg tatcaaatgg tgtaaaggaa gctcttaaaa caaattcgga
      721 ttctgtaaaa gatattgcga aaacgttatc tcgtggatta ttaatgggag cacgcggtaa
      781 ctcaggtgtt attacttcac agatttttag aggaatctat caagctgtgg aagatttaga
      841 tgaaatggat gcatcacaat tggctcacgc tttagtgaat ggtagtcgtg ttgcttatcg
      901 tgctgttatg cgtccagtag aaggtacgat tttaacagtc gttcgtgaag ctgcagacta
      961 tacatatgca tatactacta cagaagaagt aacagactgc atggatgtga tgaaaaagat
     1021 ggtggatgaa gccaatgctt ctttacaaag aacacctgaa ttactacctg ttcttgcaga
     1081 agtaggtgta gttgatagtg gtggaaaagg actttgcgta atcttagaag gtttccttgc
     1141 agctatggaa ggtcatccag ttcaacttga aggtgcaaaa gcagctggtg aaagtgcgca
     1201 ggcaaaagtt gaaggtggag aagaagaata tggattctgt acagaattta ttttgaaatt
     1261 atctgaaaat ggtattcgtc atttctcaga aactagcttt aaagatgaat tagctacaat
     1321 tggtaattca attgtttgtg tacaagatga ggatttagta aaagttcatg ttcatacatt
     1381 agaaccattt acagctatta aaatgggacg tcgtcagggt cgttttatta aattaaaagt
     1441 tgaaaatatg caagaacagc atgacaacat tattgaaaaa gaagaagagg aaaaaatggc
     1501 agaacacaaa aaatatgcca ttattaccgt agctcctggt gatggaatca agaagatgtt
     1561 tacagaactt agagctgata ttgttgtcgg tggtggacaa acaatgaatc ctagtacaga
     1621 agattttgtt tcagccgttg aaaagttgaa tgcagatcat atcttcattt tgccgaacaa
     1681 ctcaaatatt gttttagctg caagtcaggc tgcacaagta tgttccaatc aagatattca
     1741 tgtacttcca agtaaaacaa ttccacaagg attaagtgct tgtgttatgt ttaatccaga
     1801 tgttgaatta gaagataact tagctgaaat gaatgaggct attgaaaatg ttaaatctgg
     1861 tgaagtgact tatgcgatca aagatacaac atatgatggt cttgaaatta aaaaagatga
     1921 atatatgggt atttttggaa aggatatttg tgtatcttgt ccagattgta tggaagcaac
     1981 aaaaaaattg ttggataaaa tgttagatga cgatagtgaa cttgttactt tgatttatgg
     2041 tgatacagct acagaagaac aggctgaaga aatcgctgct tatatcgaag ataacagtga
     2101 tgcagaagtt gaaatccatg aaggaaatca acctgtttac tcatttatta ttggtgttga
     2161 atagtaaaat aatgaatcca tcagattgtg ttctgatgga tttttttacg caataaattt
     2221 ggaagggtta ggtgaatctg cttttcaaga atttaaggga gcggcatata cccaaaacgg
     2281 tttatagcta atatttggtt gttcgggttc ttttttaaac catattaacg ctttatcatt
     2341 cttgacgatt tcaaaatcaa tgctttttct aaaaacagca tgcatctgtt tatgcattat
     2401 ttt
//
LOCUS       DBMDWL010000249         2379 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_21186_length_2379_cov_1.571115, whole genome shotgun sequence.
ACCESSION   DBMDWL010000249 DBMDWL010000000
VERSION     DBMDWL010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21186_length_2379_cov_1.571115"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            165..473
                     /locus_tag="ACZPDN_11570"
     CDS             165..473
                     /locus_tag="ACZPDN_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR1299292.1"
                     /translation="MATKSANLYARIEPDVKENAESILSALGISASSAINMSYKQIIL
                     QRGLPFEVKIPSTRPVDINTLSEVEFNEELEKGYADMQAGRTKNAKKAFADIRKDYGL
                     "
     gene            470..703
                     /locus_tag="ACZPDN_11575"
     CDS             470..703
                     /locus_tag="ACZPDN_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR1299293.1"
                     /translation="MIYEVEVSQQADSDLREIFEYIAFELQSPKNASGQLDRLEKQIL
                     SLNTMPERYRRYEKEPWKSRGLRVLPVDNYVVL"
     assembly_gap    907..916
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1032..1418
                     /locus_tag="ACZPDN_11580"
     CDS             1032..1418
                     /locus_tag="ACZPDN_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1299294.1"
                     /translation="MRYYPNYFDDFFNDTKKQDTNTVLRTDIYEKDGYYQICMEVCGI
                     QKENIQIELKDGYLKITATRNINKEDTRVLRQERFTGTYSRSFYIGEGYKQEDIKARF
                     DNGELFILLPTKPVKHEEETRYINIQ"
     gene            1481..2362
                     /locus_tag="ACZPDN_11585"
     CDS             1481..2362
                     /locus_tag="ACZPDN_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1299295.1"
                     /translation="MSVLDENVAIFKYFEEISKIPHGSFHEEKIADYVENFAKEHDFK
                     YVRDDMNNVIIYKDATPGYEHHDTVMLQAHMDMVCEKNKDVDFDFETDALNLYVEDGW
                     LKAKGTTLGADDGFGVSYMLAILSDDTLKHPNLECVFTVQEEVGLLGSINLKKEYFNA
                     KKMIGLDGGGEISTCTTTSGGRYAYVNKTCAFEEVDKPTYKLTVKGLDGGHSGACIDQ
                     EKGNSIKILFRVLQTLKDIQIVSVNGGLKENAIPREAEATFVSDIKPNIEQITADLKI
                     EFEFSDSKLDILFLNRL"
BASE COUNT          888 a          336 c          434 g          711 t
ORIGIN      
        1 gatcttcact atttcaagct agcctattgc gtccggtgta aaagatgtat tactacagtt
       61 attcaacaag atttttggaa cgcaaaatca gtattgctaa tgtacggaca tctgcatata
      121 ataaagatag atggaaacac tactcacgaa aggagatgtt ttatatggca acaaaatcag
      181 caaatttata tgcaagaatt gaaccagatg ttaaagaaaa cgcagaaagt atcctgtctg
      241 cacttggtat ctctgcgtcc agtgctatca atatgtctta taaacaaatc atcttacaaa
      301 gaggacttcc atttgaagta aaaataccat ctaccagacc ggttgatatc aacacattat
      361 cagaagtaga gttcaatgaa gaattggaaa aaggatatgc agatatgcaa gctggacgga
      421 caaaaaatgc aaaaaaagcc tttgctgaca ttcgcaaaga ttatggttta tgatatatga
      481 agtagaagta tcccaacagg ctgacagtga tttgagagag atttttgaat atattgcttt
      541 tgaattacaa tcaccgaaaa atgcaagtgg acaacttgac cgtttggaaa aacagatatt
      601 aagtctgaat acgatgccag agcgttatcg aagatatgaa aaagagccat ggaaaagccg
      661 tggacttcgt gtattaccag tagataatta tgtcgtacta taatattcca gattgtgata
      721 aaaaggttgt aacaatactt agagtcatgt atacccgaag ggacatagac catcagttaa
      781 atctttacac aaagtaataa agtacataat ttaaaacagt ctttcattta tgattgactg
      841 ttttttttca tattcaatta attaggtgat caacaatcaa aaaaagtgtt gcaaaacccg
      901 taaaatnnnn nnnnnntaaa atcaaaattt aaacgaatct caaaattatt agcactcaga
      961 tattgactgt gccaaacaat tgtactatga tattagcagt taaaagatat aagtgctaag
     1021 gaggattcaa tatgcgctat taccctaatt attttgatga ctttttcaat gatactaaaa
     1081 aacaagatac aaatactgta ttaagaacag acatttatga aaaggatgga tattatcaaa
     1141 tctgcatgga ggtttgtggt attcaaaaag aaaatattca aattgaattg aaagatggat
     1201 atctaaagat cacagcaact cgtaatatca acaaagaaga tacgcgtgta cttcgtcaag
     1261 aaagatttac tggaacttat tctcgttctt tctatattgg tgaaggctac aaacaagagg
     1321 atatcaaagc tcgttttgac aatggtgaat tatttatctt attgcctaca aaacctgtaa
     1381 aacatgaaga agaaacaaga tatatcaata ttcaatagtg agagacttgc gtctctcatt
     1441 tttgaattat actattttta atcaagtgga ggtataaaca atgagtgtat tagatgaaaa
     1501 tgttgcgatc ttcaaatatt ttgaagaaat ctcaaagatt cctcatggat cttttcatga
     1561 agaaaaaatc gcagactatg tagaaaactt tgcgaaggaa catgatttta aatatgttcg
     1621 tgatgacatg aataatgtga ttatttataa agatgctaca ccaggctatg aacatcacga
     1681 tactgtaatg ttgcaagcac atatggatat ggtatgtgaa aaaaacaagg atgtcgattt
     1741 tgactttgaa accgatgctt taaatttata tgttgaagat ggatggttaa aagcgaaagg
     1801 aacaacatta ggtgccgatg atggatttgg agtgagctac atgttggcca ttcttagtga
     1861 tgatactcta aaacatccaa acttagaatg tgtatttact gtacaagaag aagtaggtct
     1921 attaggatct attaatttaa agaaagaata cttcaatgcc aaaaagatga ttggtcttga
     1981 tgggggtggt gaaattagta cttgtaccac aacaagtggt ggtcgctatg cctatgtcaa
     2041 taaaacatgt gcttttgaag aagttgataa accaacctac aaacttactg taaaaggatt
     2101 agatggaggt cattcaggtg cttgcattga tcaagaaaag ggtaactcca ttaaaatctt
     2161 atttcgagtt ctacaaacat taaaagatat tcaaatcgta tctgtaaatg gtggtctaaa
     2221 agaaaatgca attcctagag aagcagaagc aactttcgtt tcagatatta aaccaaatat
     2281 tgaacaaatc acagccgatt taaaaataga atttgaattt agtgatagta aacttgatat
     2341 tctattttta aatcggctgt gatttgttca atatttggt
//
LOCUS       DBMDWL010000250         2372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_21271_length_2372_cov_2.827389, whole genome shotgun sequence.
ACCESSION   DBMDWL010000250 DBMDWL010000000
VERSION     DBMDWL010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21271_length_2372_cov_2.827389"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(146..340)
                     /locus_tag="ACZPDN_11590"
     CDS             complement(146..340)
                     /locus_tag="ACZPDN_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin"
                     /protein_id="HIR1299296.1"
                     /translation="MEKKQIPLRLSKKLYDQIASWVEDDFRSVNGQIEYLLTECVKQR
                     KKNGKYVSDMMDEPIELDIK"
     gene            complement(340..1338)
                     /locus_tag="ACZPDN_11595"
     CDS             complement(340..1338)
                     /locus_tag="ACZPDN_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1299297.1"
                     /translation="MTEKVLSNKKNGMAMMILWIVLYLVALLMTIFGAAFVWPLFIIG
                     VVWLCVGWIPFLGLKVLRPQEALVLTLFGKYVGTLKDAGFYYVNPFCQAVNPAAKTKL
                     NQSGDVDDGSKKSIFQAQNNGTVEMASKKVSLKIMTLNNNRQKINDCLGNPVEIGIAV
                     MWRVTDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVAENVDTTGDGIADEGSLR
                     GSSEVVASRIRDEIQCKVKDAGLEIIEARITYLAYAPEIAAVMLQRQQASAIIDARKM
                     IVDGAVGMVEMALDRLNENGVVELDEERKAAMVSNLLVVLCGNHDAQPVVNSGSLY"
     gene            complement(1404..2165)
                     /gene="aroD"
                     /locus_tag="ACZPDN_11600"
     CDS             complement(1404..2165)
                     /gene="aroD"
                     /locus_tag="ACZPDN_11600"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR1299298.1"
                     /translation="MNTVTMKNVEFGAGVPKICVPICGKSKEDVLEQAKIILDTPADI
                     IEWRMDWFEQVENTASVVEMAKELREVFKETPVLATFRSKKEGGELEVSTEYYVELNC
                     AVAKSGYVDAVDVELYTGNKEVETIVTTAHENGVKVIMSNHDFFKTPSKEEIISRLCT
                     MQEKGADIPKIAVMPQSKRDVLTLLSATNEMAEEHADRPIITMSMAATGVISRLAGEV
                     FGSCLTFGAAKKASAPGQMGVNDLKTVLETLHKSL"
BASE COUNT          731 a          521 c          308 g          812 t
ORIGIN      
        1 cagttttaac ctttaaaagt agcttaaatt actaagtttt tcagttttaa cctttaaaag
       61 tagcttaaat tactaagttt ttcagtttta acctttaaaa gcttttattt tttccaaaaa
      121 taaaagaatg tatttctaca ctctcttact taatatccaa ttcaattggc tcatccatca
      181 tatcactgac gtatttgcca ttcttttttc tttgtttcac acactctgtc aacaagtatt
      241 caatttgtcc attaacactg cgaaaatcat cttctaccca tgaagcgatt tgatcataca
      301 atttttttga aagacgcaaa ggaatctgtt tcttttccat taatacaaac tgccagaatt
      361 tacaactggc tgcgcatcat ggtttccaca aagtacgact aacaagttag aaaccatagc
      421 cgctttcctt tcttcatcca attctacaac cccattttca tttaaacgat ccaatgccat
      481 ttcaaccatg ccaacagctc catccacaat catcttacgt gcatcaataa tggcacttgc
      541 ctgttgtctt tgaagcataa cggctgcaat ttctggtgca tatgccaaat atgtaattct
      601 tgcctcaata atttcaagac ctgcatcttt tactttacat tgaatttcat cacgaattct
      661 agaagcaacc acttcacttg aacctctaag acttccctca tcggcaatac catctcctgt
      721 tgtatctaca ttttcagcga catcataagg ataaatacga acaatatttc ttaatgcact
      781 atcgcattgt aaagaaagat attccttata gttatctaca ttaaatacag cttttgcggt
      841 atctgtaaca cgccacatca cggcaattcc aatctcaaca ggatttccta aacaatcatt
      901 gattttttgg cgattgttgt ttaaagtcat gattttcaaa gatactttct ttgaagccat
      961 ttctactgtt ccattatttt gtgcttgaaa aatactcttc ttagaaccat catccacatc
     1021 tccgctttga tttagttttg tctttgcggc aggattgaca gcctgacaaa agggattgac
     1081 ataatagaat ccagcatctt ttaaagtacc tacatattta ccaaataatg ttaaaactaa
     1141 agcttcttgt ggtcttaaca cctttaaccc aagaaacgga atccaaccca cacatagcca
     1201 tacaacacca ataataaata aaggccaaac gaaagctgcc ccaaatattg tcatcaacaa
     1261 ggcaacaagg tataaaacaa tccataatat catcatggcc atgccattct ttttgttgct
     1321 taaaactttt tctgtcatga caagcacctc cactatctaa atgatatcat tttgatatcg
     1381 tatgtcaata aaaaaaagac aggttataaa cttttgtgta aagtttcaag aactgtctta
     1441 agatcattga cacccatttg acctggagca cttgcttttt tagcagcacc aaaagtcaaa
     1501 caagaaccaa atacttcacc agctaaacga gaaataacgc cagttgctgc catagacatt
     1561 gtgatgattg gacgatcagc atgttcttct gccatttcat ttgtggcaga caataatgtc
     1621 aatacatccc ttttagattg aggcataact gcaatcttag gaatgtctgc acccttttct
     1681 tgcatagtgc ataagcgaga aatgatttct tcttttgatg gtgttttaaa gaagtcatgg
     1741 tttgacataa tgacttttac tccattttca tgcgctgtcg ttacaatcgt ttcaacttcc
     1801 ttatttcctg tatataattc aacatctact gcatctacat aaccgctttt ggcaactgca
     1861 cagtttaatt ccacataata ttcagtagat acttctaatt ctccaccttc ttttttagaa
     1921 cgaaaagtag ctaagactgg ggtttcttta aatacttcac gtaattcttt agccatttct
     1981 actacagatg cagtattttc aacttgttca aaccaatcca tacgccattc aataatatca
     2041 gctggtgtat caagaatgat tttagcttgt tctaaaacat cttctttact ctttccacaa
     2101 ataggaacac aaatctttgg tacacctgca ccaaattcaa catttttcat tgttactgta
     2161 ttcattttta ttctccttta ttcatatttt ttatatacgc atcaactgac tactcctcac
     2221 cacaagcagt gagggttctt tactgacaac attatgcagc aatcctacat gattttcaaa
     2281 ctttggattc ctgtctttct gttggagcag tgaactctac aatcaagcac tacatttagc
     2341 acattagcga ttgttttctg tatctctaca tg
//
LOCUS       DBMDWL010000251         2371 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_21292_length_2371_cov_2.700000, whole genome shotgun sequence.
ACCESSION   DBMDWL010000251 DBMDWL010000000
VERSION     DBMDWL010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21292_length_2371_cov_2.700000"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /gene="mutL"
                     /locus_tag="ACZPDN_11605"
     CDS             complement(<1..818)
                     /gene="mutL"
                     /locus_tag="ACZPDN_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1299299.1"
                     /translation="MAKIHVLSEHLTNMIAAGEVVERPAGIVKECVENSIDAGATVIE
                     VEVFQGGIERIVITDDGCGMDHEDAHLAFMRHATSKMHSEEELFNIQTMGFRGEALPS
                     IASVAQVELQTSNGEEGSLLRYNYGSLDVDEKCNCPRGTKLDVSGLFIKTPARFKHLK
                     STSYEFSVIADLMNKMALSHPDIRFQLSHDGRVVFQTSGNGNIQEILYQMYGKEVAQN
                     AIHFEGSNEDFHIHGYAIQPKINRATKYFMFLTLNTRLIRSVAIQKAILDAYSD"
     gene            complement(851..>2371)
                     /gene="mutS"
                     /locus_tag="ACZPDN_11610"
     CDS             complement(851..>2371)
                     /gene="mutS"
                     /locus_tag="ACZPDN_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR1299300.1"
                     /translation="RLASRMAYGSASPRDVLQLVATLEHAKPILDLAISLDSYPEFSQ
                     VPDCQELYNEIKNAIVENPPLTLKEGGVFNDGYNQELDEVRRIAKKGKDFILELEAKE
                     RERTGVKSLKVGYNRIFGYFIEVRNGNLSNIKDEFGYHAKQTLANATRFVTQELKEKE
                     EQILHAQETKVKLEQSLFNDLLLRIKKDLFDLHACAQALSTIDVLVSLAILASEKGYV
                     CPVFHPGYNVNVVEGKHPILDDRMKKKRYVSNDWKMSEDEHVQLITGPNMGGKSTYMR
                     QNALLVVMAQMGSFIPAKTAELPIFDRIFTRIGASDDILTGKSTFMVEMMEANAALCY
                     ATKHSLILFDEIGRGTATYDGMALAQAMLEYIDEAIGAKTLFSTHYHELTDLEKEHPS
                     MHNVHVDVREEKNEIEFRYRIIDGKADKSYGINVARLAHLPKVVLDRAAQLLTNFENQ
                     DNNQNYQPSLFVMEQVQPEKSKLLQQLQELDIDSMTPRDALDCLYELKKLSEKIEL"
BASE COUNT          709 a          483 c          312 g          867 t
ORIGIN      
        1 taatcactat aagcatctag aatcgctttt tggattgcga cactacgaat caatcttgta
       61 tttagagtta aaaacataaa atattttgtt gcacgattta tttttggctg aatcgcatat
      121 ccatgaatat gaaaatcttc attacttcct tcaaaatgaa ttgcattctg tgctacttct
      181 ttcccataca tttgatatag aatttcttga atatttccat tcccactcgt ttgaaataca
      241 actcgcccat catgagaaag ttgaaaacga atatccggat gtgataatgc cattttattc
      301 attaaatctg caatgacaga aaattcatat gaagtacttt tcaaatgttt gaatcgagcc
      361 ggtgttttta tgaacaatcc agaaacatct aattttgttc ctctaggaca attacatttt
      421 tcatccacat ccaaacttcc atagttatat cttaataagc taccttcttc accattgctc
      481 gtttgtaatt cgacttgggc aacagaagca atacttggta atgcctctcc tctaaatccc
      541 atcgtttgaa tattgaacaa ttcttcttca gaatgcattt tacttgtggc atgtcgcata
      601 aaagctaaat gtgcatcttc atgatccatt ccacaaccat catctgtaat cacaattctt
      661 tcaataccac cttgaaagac ttcaacttct atgactgtag cacctgcatc aatactattt
      721 tcaacgcatt cctttacaat tccagctggt ctttcaacaa cttctccagc cgctatcata
      781 tttgttaaat gttcactcaa aacatgaatc ttagccatac ttcacacctc cattcatatg
      841 aatttcatat ttataattcg attttttcac ttagcttctt taattcatac agacaatcca
      901 gagcatctct tggtgtcatt gaatcaatat ctaattcttg taattgttgt aaaagtttgg
      961 acttttctgg ttgcacttgt tccatcacaa ataaacttgg ttgataattt tgattgttat
     1021 cttgattttc aaaatttgtt agaagttgtg ccgctctgtc caaaacaact tttggtaaat
     1081 gcgcaagacg cgcaacattg ataccatacg acttatctgc ttttccgtca ataatacggt
     1141 atctaaattc aatttcattt ttttcttcac gaacatccac atgaacattg tgcattgatg
     1201 gatgttcttt ttctaaatct gttaattcat ggtagtgtgt tgaaaataat gtttttgcac
     1261 caatagcttc atcaatatat tcgagcattg cctgtgcaag agccatacca tcgtatgttg
     1321 ctgttcctcg accaatttca tcaaacaaaa ttaaagaatg ttttgttgca taacacaaag
     1381 cagcatttgc ttccatcatc tctaccataa atgtactttt tccagttagt atgtcatccg
     1441 aagcaccaat tcttgtaaaa atgcgatcaa aaataggtaa ttctgctgtt tttgcaggaa
     1501 taaaacttcc catttgtgcc attaccacca ataaagcgtt ttgtcgcata taagttgatt
     1561 taccacccat gtttggccct gtaattaatt gaacatgttc atcttctgac attttccaat
     1621 cattcgatac atatcttttt ttcttcatac gatcatctaa tatcgggtgt tttccttcta
     1681 ctacattgac attgtatcca ggatgaaaaa ctggacatac atatcctttt tcactagcaa
     1741 ggatcgccaa agaaactagt acatcaattg tagacaatgc ttgtgcacac gcatgaagat
     1801 caaataaatc tttttttatt cttaataata aatcattaaa caaactttgt tccaacttca
     1861 cctttgtttc ttgtgcatgt aaaatttgtt cttctttttc ttttaattct tgtgtaacaa
     1921 atcttgttgc attagcaagt gtttgtttcg catgatatcc aaattcatct ttgatatttg
     1981 ataaattccc attacgaact tcaataaaat agccaaaaat acgattgtat ccgactttta
     2041 atgattttac acccgttctt tcgcgttctt tagcctcaag ttctaaaata aaatctttcc
     2101 cttttttggc aattcttctt acttcatcca attcttgatt ataaccatcg ttaaatacac
     2161 caccttcttt tagtgttagt ggcggatttt ctacaatcgc atttttaatt tcattatata
     2221 attcctggca atctggcact tgagaaaatt ctggatatga atccaaagaa atagctagat
     2281 ccaatatagg ttttgcatgt tctagtgtag caacaagttg taaaacatct ctaggacttg
     2341 cactaccata agccatacga cttgcaagtc t
//
LOCUS       DBMDWL010000252         2360 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_21441_length_2360_cov_1.841523, whole genome shotgun sequence.
ACCESSION   DBMDWL010000252 DBMDWL010000000
VERSION     DBMDWL010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21441_length_2360_cov_1.841523"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            253..441
                     /locus_tag="ACZPDN_11615"
     CDS             253..441
                     /locus_tag="ACZPDN_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299301.1"
                     /translation="MDTDIEKAVAYLHDVLEDTSVTVDELRNMFSNEIVDGVITLTHR
                     KDESYFEYISMSIYIKVS"
     assembly_gap    549..558
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            761..2140
                     /locus_tag="ACZPDN_11620"
     CDS             761..2140
                     /locus_tag="ACZPDN_11620"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1299302.1"
                     /translation="MNEKTFNDVVNHMKTSGFVYQGSEIYGGLANTWDFGPLGVELKN
                     NIKNAWWKRFIQESEHNVGLQSAILMNPNVWVASGHVGGFSDPLMDCKECKSRFRADQ
                     LIEEASNHTVNCGGMSNEDMENYIAEHEITCPKCGAKNFTNIRQFNLMFKTFQGVLED
                     AKSTIYLRPETAQGIFVNFKNVQRTMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFE
                     QMELEFFCKPGTDLDWFEYYCKNCVQFLKDLGINEENIRLRAHDPEELAHYSNGTSDI
                     EYHFSEPIGWGELWGIADRTDFDLKAHQTTSKQNLEYFDQEANEKYIPYVIEPSVGVE
                     RLMLAIMCEAYQEEQLENDSRVVLKLHPALAPYKVAVLPLSKKLSDQGDEVFAKLSKH
                     FSVTYDDAQSIGKRYRRQDAIGTPFCVTVDFETANDGCVTIRDRDTMQQERVALDDLV
                     SYIENRIVF"
     gene            2140..>2360
                     /locus_tag="ACZPDN_11625"
     CDS             2140..>2360
                     /locus_tag="ACZPDN_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009300025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR1299303.1"
                     /translation="MSWISEEDIKAIRQQADIVDVMSHYITLEKQGKNYKAICPFHDD
                     HDPSLSISTDKQIFKCFVCGTGGNVFTFV"
BASE COUNT          846 a          352 c          456 g          696 t
ORIGIN      
        1 atcgagcttt gtttaaatgt aaagaagaga acttagctta tgtcatagaa catttcacat
       61 ttaaccttga cgatttgatc tatctagcta aatggatcga atatcatagt caaaaaagct
      121 atccaagcat tcggttttta gaaagtataa gaggtttaga gtgatgtttc aagaattagc
      181 acttcaagtt acacaaaaag cacatgcagg tcaagttgat aaagcaggaa aagattatat
      241 cctgcatcct gcatggatac agatatagaa aaggccgttg cgtatttgca tgatgtatta
      301 gaagatacaa gtgttactgt ggatgaatta agaaatatgt tttcaaatga aatcgtagat
      361 ggcgtaatca cattaacgca tagaaaagat gaatcttatt ttgaatatat atctatgagt
      421 atctacatca aagttagcta aaaaggtaaa gactgcagat ctttatatca caagaatcaa
      481 agaaccaaca aaaacggatt atgaaagact cgaaaaatac aagaaagcga tactgtatcg
      541 agtgatgcnn nnnnnnnntg atgcactagt gttgtggtac aaaacatatg aaaataagag
      601 aacctatttt taagtggggg attaaaaaca ggccatgaga aatcgtggct ttttctttat
      661 gtgtgtcttt ggatttagtc tagaaattta ttgataaata ggatataata tgaaagtgta
      721 tccttttgta tgtatacata aaagaaatga ggtaaataac atgaacgaaa agacattcaa
      781 tgatgtagta aatcacatga aaacctctgg ttttgtttac caaggttcag aaatctatgg
      841 tggtttggca aatacttggg attttggtcc tttaggcgtt gaattaaaga acaatattaa
      901 aaatgcttgg tggaaacgtt tcatccagga aagtgaacac aatgtaggat tgcagagtgc
      961 aatcttaatg aaccctaatg tatgggttgc cagtggtcac gttggcggat ttagtgatcc
     1021 attaatggat tgtaaagaat gtaagagccg ttttagagcg gatcaattga ttgaagaagc
     1081 cagcaatcac acagtaaact gtggtggtat gtcaaatgaa gatatggaaa actatattgc
     1141 tgaacatgaa atcacatgtc caaaatgtgg tgctaaaaac ttcacaaata ttcgtcaatt
     1201 taacttgatg ttcaaaacat tccaaggtgt attagaagat gcgaaaagta cgatctattt
     1261 acgtccagaa acagcacaag gtatctttgt caacttcaaa aacgtacaaa gaacaatgcg
     1321 taaaaaatta ccattcggta ttggacaaat tggtaagagt ttccgtaacg aaatcacacc
     1381 aggaaatttc attttccgta ctcgtgaatt cgaacaaatg gaacttgaat tcttctgtaa
     1441 accaggaacc gatttagatt ggtttgaata ctattgtaaa aactgtgttc aattcttaaa
     1501 agatttaggt attaatgaag aaaacattcg tttacgtgct catgatcctg aagaattagc
     1561 acactactca aatggtacaa gtgatatcga ataccacttc tctgaaccta tcggatgggg
     1621 tgaattatgg ggtatcgcag accgtacaga ctttgactta aaagcacacc aaacaacttc
     1681 taaacaaaac ttagaatatt ttgatcaaga agccaatgaa aaatatattc cttatgttat
     1741 tgaacctagt gtaggtgttg aacgtttgat gttagctatt atgtgtgaag cttatcaaga
     1801 agaacaatta gaaaatgatt ctcgtgttgt attaaaatta caccctgcat tagcaccata
     1861 taaagtagct gtacttccat tatctaaaaa attatctgat caaggggatg aagtattcgc
     1921 aaaattatca aaacacttta gcgtaacata tgatgatgct caaagcattg gtaaacgtta
     1981 tcgtcgtcaa gatgcgattg gtacaccatt ctgcgtaact gttgactttg aaacagctaa
     2041 cgatggatgt gtaacaatcc gagatcgtga cactatgcaa caagaacgcg tcgctttaga
     2101 tgatcttgtt tcatatatcg aaaatagaat cgtattttaa tgagctggat tagcgaagag
     2161 gatataaagg caatacgtca acaagccgat attgttgacg taatgtctca ctatatcaca
     2221 cttgaaaaac aaggcaaaaa ttataaagcc atatgtcctt ttcatgatga tcatgatcca
     2281 agcttatcga tttcgacaga taaacaaata ttcaaatgtt ttgtgtgtgg cacaggtgga
     2341 aatgtcttta catttgtgca
//
LOCUS       DBMDWL010000253         2351 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_21562_length_2351_cov_2.404444, whole genome shotgun sequence.
ACCESSION   DBMDWL010000253 DBMDWL010000000
VERSION     DBMDWL010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21562_length_2351_cov_2.404444"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            121..732
                     /locus_tag="ACZPDN_11630"
     CDS             121..732
                     /locus_tag="ACZPDN_11630"
                     /inference="COORDINATES: protein motif:HMM:NF025711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HIR1299304.1"
                     /translation="MEKKIKLVCPECNKEFNTDIYTSINVQLDKDMKTKILSGKLFDI
                     ECEHCHSKFHIPYPVLYHDMEKKLLIQYTEEKDLKSSEIALKNANVDTDYTVRIVDNE
                     RDWIEKILISDSGFDDRIMELYKLLILSQYEDAENVNGLYYWNIQTLENPHYVMVLDE
                     KESDKMHFMPFHEGVYKQVEEVFLSDIQQDYIIDANWAISNTK"
     gene            741..1472
                     /locus_tag="ACZPDN_11635"
     CDS             741..1472
                     /locus_tag="ACZPDN_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299305.1"
                     /translation="MIASIHACVDLLHVGYRVKEEYDPIILNVYHCLKVEGLNDEKYM
                     LHIDSVLKKSIHDLDETQICTYITYVFEKEKYVPYFLKSFIESGMFKELLLRYLSLKE
                     SDTLQCIKLNRNLLSKWHYASVSEEVAQNLTISANGRVYLTRYIMTNEGVKPLKTQQA
                     SISKIEANEILDAISKLDLESKEVEENGWQLKMYKSNCGKNRLVKTIEHDAKNPDGLN
                     ERIRSILPFEHLFVFGGSYYSWEKM"
     misc_binding    1522..1727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1760..>2351
                     /locus_tag="ACZPDN_11640"
     CDS             1760..>2351
                     /locus_tag="ACZPDN_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1299306.1"
                     /translation="MQQIQLPQGMKDTILQECEKKKYLQNTLEQIFESFGYEEIITPT
                     IEYYETYENAFKNIQTTDMYKFFDENGRILTLRTDMTVPIARVCASKFKDSKPPFRYR
                     YTSNVFKVRQKFAGKRSEVTDCGIELIGLDSKSDVQVLCCALEVMKNFKNYTLEIGNV
                     QFFQAACKVVRLSTEEINTLADLIDKKSMVDLKIYLD"
BASE COUNT          892 a          277 c          441 g          741 t
ORIGIN      
        1 aatgtctttt acagaacttg aagtagccaa ccgagaatta aaaattgctc ttcaaaaaga
       61 atatgaagat tgttttgtgg aacttgttcc caatgtttta gcgaataaat aggagaaatt
      121 atggaaaaga aaattaagtt agtatgtcct gaatgtaata aggaatttaa tacagatatt
      181 tatacttcaa tcaatgtgca attggacaaa gatatgaaga caaagatact gagtggaaag
      241 ctttttgaca tagaatgcga acattgtcat tctaagtttc atataccata tcctgtttta
      301 tatcacgata tggaaaagaa gcttttaatt cagtataccg aagaaaaaga cttaaaatcg
      361 agtgaaatcg ctttaaaaaa tgccaatgta gatacagatt atacagtgcg tatagtcgat
      421 aatgaacgag actggattga aaaaattttg atttcagata gtggatttga tgatcgcatt
      481 atggaattgt acaagctttt aatactttca caatatgaag atgctgaaaa tgtgaatggt
      541 ttatattatt ggaatattca aacattagaa aatcctcatt acgtaatggt gttggatgaa
      601 aaggaaagtg ataaaatgca ttttatgcca ttccatgaag gtgtatataa acaagtagaa
      661 gaagtattct tatccgatat tcaacaagac tatattattg atgcgaactg ggcaatttcg
      721 aatacgaagt aggtgatgtc atgattgcaa gcattcatgc gtgtgtggat cttttacatg
      781 tgggctatcg tgtaaaagaa gaatatgatc ctattatttt aaatgtatat cattgtttaa
      841 aagttgaagg tttaaacgat gaaaaatata tgttacatat tgattctgta ttaaagaaaa
      901 gtattcatga cttggatgaa acacaaattt gtacatatat tacatatgtg tttgaaaaag
      961 aaaagtatgt accttacttt ttaaaaagtt tcattgaatc aggaatgttt aaagaattat
     1021 tacttagata tctttcacta aaagaatcag atacactaca atgtattaaa ttaaatcgaa
     1081 atctattaag taaatggcat tatgcaagtg tatctgaaga agttgctcaa aacttaacaa
     1141 tcagcgcaaa tggtagagtc tatctaactc gatatatcat gacaaatgaa ggtgtaaaac
     1201 cactaaaaac acagcaggca agtatatcga aaatagaggc gaatgaaata ttagatgcga
     1261 tatcaaagtt ggatttagaa tcaaaagaag tcgaagaaaa tggctggcaa ttaaaaatgt
     1321 ataagagtaa ttgcggaaaa aatcgccttg tgaagaccat tgaacacgat gcgaaaaatc
     1381 ccgatggatt gaatgaaaga attcgttcaa ttcttccttt tgaacattta tttgtatttg
     1441 ggggatccta ttattcatgg gagaaaatgt aaaatattta catttattat tgacatttat
     1501 tttcaatgat actatattat tttcaaagca atgaaaagaa atagtaggat agtactgctt
     1561 taagagactt tctggttggt gcaaagaaag agtaaggcta tttgaacacg tcttggagct
     1621 gtaaagatga aaatagtagt ttttaacgta ttcctggcgt tatcaggttt gaggctatga
     1681 atagcgaaat aaggtggaac cacgtaagtt aaactttcgt ccttaggacg gaagtttttt
     1741 tgtttatagg gaggtcatta tgcagcagat acaattacca caaggaatga aagataccat
     1801 tttacaagaa tgtgaaaaga aaaaatatct acaaaatact ttagaacaaa ttttcgaatc
     1861 ttttggatat gaagaaatca ttacaccaac gattgagtat tatgagacat atgaaaatgc
     1921 atttaaaaat attcaaacga cagatatgta taaatttttt gatgagaatg gaagaattct
     1981 aacattaaga accgatatga ctgtccctat tgcacgtgta tgtgcaagta aatttaaaga
     2041 ttcgaagcct ccatttcgct atcgatatac ttcaaatgta tttaaagtac gacaaaagtt
     2101 tgccggtaaa agaagtgaag ttacagattg tggcattgaa ttaattgggt tggattcaaa
     2161 aagtgatgtt caagtattat gttgtgcttt agaagtgatg aagaatttta aaaactacac
     2221 attggaaatt ggaaatgtac aattttttca agccgcatgt aaagtcgttc gcttaagcac
     2281 agaagaaatt aatactttag cggatttgat tgataaaaag agtatggtcg atttaaaaat
     2341 atatttagat t
//
LOCUS       DBMDWL010000254         2330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_21852_length_2330_cov_1.693136, whole genome shotgun sequence.
ACCESSION   DBMDWL010000254 DBMDWL010000000
VERSION     DBMDWL010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21852_length_2330_cov_1.693136"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>427)
                     /locus_tag="ACZPDN_11645"
     CDS             complement(<1..>427)
                     /locus_tag="ACZPDN_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299307.1"
                     /translation="VVEKGYHLGPLIHQKRTNALENAILSIQKYLPKNCSKQEIIICV
                     KQHDEALKEYKKLLIMQTPYRLLSSFLVDVGGNDPIWNRPKDIIETIKDYNEKYRLPY
                     IIENDRGLKRRVVVQPEWRDFLMTNYRVIMEWVHDEKIK"
     assembly_gap    428..437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<438..643)
                     /locus_tag="ACZPDN_11650"
     CDS             complement(<438..643)
                     /locus_tag="ACZPDN_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299308.1"
                     /translation="MLDVERLQFLDLYSFELRLDYFEKILEFTSSSYSFYWLEAVLNL
                     MIYRDTIEFDEILDEMISLAYDDVV"
     gene            complement(648..968)
                     /locus_tag="ACZPDN_11655"
     CDS             complement(648..968)
                     /locus_tag="ACZPDN_11655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIR1299309.1"
                     /translation="MKRAVEEILKFNEERDWDQFHNPENLAKSISIEAGELLECFQWN
                     NEYDKQHVCEELADVMNYCILMANKLGVDMEEILLMKLEQNKKKYPVEKCKASSKKYT
                     ELIK"
     gene            complement(1123..2217)
                     /gene="tnpB"
                     /locus_tag="ACZPDN_11660"
     CDS             complement(1123..2217)
                     /gene="tnpB"
                     /locus_tag="ACZPDN_11660"
                     /inference="COORDINATES: protein motif:HMM:NF038281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HIR1299310.1"
                     /translation="MEKAFKYRMYPNREQRILLAKTFGCTRFVYNHYLAKRKDAYEKD
                     GITLSYSACAKDLVSLKKEYEWLKEVDSVALQSSVKNLDTAYINFFKKKAEYPRFKSK
                     KTHRYSYTTKYTNGNIELLDNKVKLPKIGLVKIKKTRAPKGRLLSATVSQVPSGKYYV
                     SLCYTDVEEVLFPLTYNETGIDLGIKDFIILSNGEKVENPKYLKKSIEKLKKLQKQQS
                     RKTKGGRNWIKNRIKIARLHEKIANQRMDFINKLSTRIITEYDIICIEDLKVDNMLKN
                     HNLAQSISDVSWSKFTTQLEYKAEWYGKVIKKVDTFYASSQICHCCGYKNEGTKDLKV
                     REWTCPKCHSNHDRDVNASINILMQGILAN"
     gene            complement(2243..>2330)
                     /locus_tag="ACZPDN_11665"
     CDS             complement(2243..>2330)
                     /locus_tag="ACZPDN_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1299311.1"
                     /translation="WNPSYFVATVSENTEEQIIEYIRSQKRK"
BASE COUNT          651 a          474 c          302 g          893 t
ORIGIN      
        1 tacttgattt tttcatcatg aacccattcc ataatgacac gataattagt cattaagaaa
       61 tctctccatt ctggttgaac aacaactctt cttttaagac ctctgtcatt ttctatgata
      121 tacggaagtc tatatttttc attataatct ttaattgttt ctataatatc cttaggccga
      181 ttccaaattg gatcattacc acctacatcc accaaaaaac tcgataataa acgatatggt
      241 gtctgcatga tcaaaagctt cttgtattcc tttaaagctt catcatgctg ctttacacaa
      301 ataataattt cttgtttcga acaattttta ggcaaatact tttgaatgct taaaatcgca
      361 ttttccaatg cgtttgtacg cttttgatga atcaatggac ccaaatgata tcctttttcg
      421 accacatnnn nnnnnnnacc acatcgtcat aggctaaaga aatcatttcg tctaagattt
      481 catcaaattc aattgtatcc ctataaatca ttaaatttaa cacagcctct aaccaataga
      541 aagaataaga agaactcgta aattctagaa tcttctcaaa ataatccaat cgtagttcaa
      601 aactatataa atctaagaat tgtaatcgct cgacatctaa cataccctta ttttatcagt
      661 tctgtgtact ttttagaaga tgctttacac ttttcaactg gatatttctt tttattttgt
      721 tctaatttca tcaacaaaat ctcttccata tccacaccta gtttgttggc cattaaaata
      781 caataattca tcacatctgc caattcttca caaacatgtt gcttatcata ttcattattc
      841 cattgaaagc attccaacaa ttctcctgcc tcaatactaa tggattttgc gagattttct
      901 ggattatgaa attgatccca atctctttcc tcattaaact ttaagatttc ttcaactgcc
      961 cttttcatct taatcctcca tcattcgata tttctttggt gattttaaat acctgattga
     1021 acctctcatc actgaagtga caagattctt gggaacaggc ttctatcgaa acctttctta
     1081 ccaagctatc cccgcagttc ctgcggttgt ttttattttt agctaatttg ctaatatgcc
     1141 ttgcatcaat atattgatgg atgcatttac atctctgtca tgatttgaat ggcattttgg
     1201 acatgtccat tctcttactt ttaaatcttt tgttccttca ttcttatatc cgcaacaatg
     1261 acagatctga ctgctcgcat agaacgtatc cactttcttg atcacctttc cataccactc
     1321 ggctttatat tctagctgtg ttgtaaactt tgaccaggat acatccgata tagactgggc
     1381 tagattgtga tttttaagca tattgtcgac ttttaaatct tcgatacaga taatgtcata
     1441 ttctgtaatg attctagtag atagtttgtt gataaaatcc attctttgat tcgctatctt
     1501 ttcatgaagt cttgcgattt tgattctgtt tttgatccag ttgcggccgc cctttgtttt
     1561 tcgtgactgc tgcttctgga gctttttcag tttctcaatc gacttcttca gatattttgg
     1621 attttctacc ttttctccat tactcaatat gataaaatcc ttgataccta gatctatacc
     1681 tgtttcatta tatgttagtg gaaataagac ttcttctaca tccgtataac ataatgaaac
     1741 ataatacttt ccacttggta cttgtgatac cgttgcacta aggagtcttc ctttaggtgc
     1801 tcgtgttttc tttatcttta ctagaccaat cttaggaagc ttgaccttat tgtctaataa
     1861 ttcaatattt ccgttcgtat attttgttgt atatgaataa cgatgcgtct tcttcgattt
     1921 gaatcttgga tattctgcct ttttcttaaa gaaatttata taagccgtat ccaaattctt
     1981 tacgcttgac tgtagagcaa cagaatccac ttccttaagc cattcatatt ctttctttaa
     2041 agaaactaga tccttagcac atgccgaata acttaacgtg attccatctt tttcataggc
     2101 atccttacgt tttgccaggt aatgattata tacaaagcga gtgcagccaa aagtcttggc
     2161 cagcaatatt ctttgttctc tatttggata catacgatac ttgaatgcct tttccatgat
     2221 gatttctcct cttattgatt cgctattttc ttttttgact tcttatatac tctataatct
     2281 gttcttctgt attttcagaa actgtagcta caaaataaga aggattccat
//
LOCUS       DBMDWL010000255         2284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_22503_length_2284_cov_1.879066, whole genome shotgun sequence.
ACCESSION   DBMDWL010000255 DBMDWL010000000
VERSION     DBMDWL010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22503_length_2284_cov_1.879066"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            107..964
                     /locus_tag="ACZPDN_11670"
     CDS             107..964
                     /locus_tag="ACZPDN_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1299312.1"
                     /translation="MNKDEALNKFTFMMEYRNLSSNTIHMYRWYLSRMFDFYQLEDVS
                     ILDVSMAQNYVVHMKQTYAPASLNAVISAIRYFFDVVLESPLSRRQFPNILYHPVEIT
                     VFTDEQIHLLLDTKDIRLRAFLLLGLDAGLRVGEVARLKVSNIDSKNMLLSIQNSKRG
                     KSRKVPLSNTLLKALREYWIVYKPDKNEYLFPAVQSNSKTPYINQSYINILFKEYIKN
                     FSFYVPTMRFHNLRDTYATLMLKNGCNIFTLKKLLGHSSFSSTSRYIKYDISDLAQTP
                     VLSSLMEIE"
     gene            983..>1454
                     /locus_tag="ACZPDN_11675"
     CDS             983..>1454
                     /locus_tag="ACZPDN_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HIR1299313.1"
                     /translation="MDIEGKIDYSKLNHKQKKALRNIIECKTEVMGFNTDTCECCGHT
                     EIHYNSCKNPNCPECGAVDKEIWIHKQERFTLNVNYFHVVFTIPNELNILCLMDPKFM
                     YKALFDVSAETIKELSKDKKYLGAKIGFTSVLHTWGQNLSLHPHIHMIVPGGGID"
     assembly_gap    1455..1464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1465..2055
                     /locus_tag="ACZPDN_11680"
     CDS             <1465..2055
                     /locus_tag="ACZPDN_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HIR1299314.1"
                     /translation="GKWKNSKKKFFLPVKVVSKLFKGKFLSYTKKNFDQRKIKDEEQF
                     QNIINTCYSKDWVVYTKKPMKSAKHVVKYLGRYTHRIAISNARLKKYEDNKVTFSCKD
                     YSDYNQIKEMTLDDTEFFRRYMMHVLPPNFMKIRHYGFLGNRNKEERIKAVRTATNTK
                     NPGPLLIDYEQIISDILKRDVSICIKCGQKRHYQLE"
BASE COUNT          822 a          372 c          367 g          713 t
ORIGIN      
        1 aacaacaccg ccttcaacca cggttattaa aggttgaaag aaaggagaag tatattgtag
       61 tcgaccagtt agatgggtca attacaatat accaggatgt gttgttatga ataaagatga
      121 agctttaaac aaatttactt ttatgatgga atatagaaat ctttcatcaa ataccattca
      181 catgtataga tggtatcttt caagaatgtt tgatttttat caattggaag atgtatctat
      241 tcttgatgtt tcaatggcac aaaattatgt tgtccatatg aaacagacgt atgctcctgc
      301 ttctttaaac gcagtcatca gtgctatccg ttacttcttt gatgttgttt tagaaagccc
      361 attatctaga agacagtttc ccaacatttt atatcatcct gtagaaatca ctgtttttac
      421 agatgaacag attcatttgt tgctcgatac aaaagatata cgacttcgtg cttttctact
      481 tcttggcttg gatgccggac ttagagttgg tgaagtggct cgtttaaaag ttagcaacat
      541 tgattctaaa aatatgttgc tttctattca aaattcaaaa cgtggtaaat ctcgcaaagt
      601 acctttatct aacacattgt taaaggctct tcgtgaatat tggatcgtct ataagcctga
      661 taagaatgaa tacttattcc cagctgttca atcgaattcc aaaactccat atatcaatca
      721 gagttacatc aatattcttt tcaaagagta cattaaaaat ttttcctttt atgttccaac
      781 catgcgtttc cataatcttc gagacacgta cgctacactt atgctcaaaa acggatgtaa
      841 tatattcact ttaaagaaac tgttgggaca ttcaagcttt tcttctacat cacgttatat
      901 caagtacgat atttctgatt tagcacagac tccagttctt tcttctttga tggaaattga
      961 gtaatggtac atattcaaat acatggatat cgaaggtaaa atcgattatt caaaattgaa
     1021 ccacaaacag aaaaaagctc ttcgcaatat cattgaatgt aaaacagaag taatgggatt
     1081 caatacagat acttgtgaat gctgcggtca tactgagatt cactacaatt catgtaaaaa
     1141 tccaaactgc cctgaatgtg gagctgtaga caaagagata tggatacata agcaggaacg
     1201 ttttactttg aatgtgaatt attttcatgt tgtatttacg attcccaatg aattaaacat
     1261 actttgtcta atggatccaa aattcatgta caaggctttg tttgatgttt ctgcagaaac
     1321 catcaaagaa ctgtcaaaag acaaaaagta tctaggagct aaaattggat ttacttccgt
     1381 acttcatact tggggacaga acctttcttt acatccgcat atccatatga tcgttcctgg
     1441 tggtggaatt gattnnnnnn nnnnggaaaa tggaaaaatt caaaaaagaa attctttctt
     1501 ccagtaaaag tagtatctaa attatttaaa ggaaaattcc taagctatac taaaaagaat
     1561 tttgatcaaa gaaaaatcaa agatgaagaa cagtttcaaa atattataaa tacctgttat
     1621 tccaaagact gggttgtata tactaaaaag ccaatgaaaa gtgcaaaaca tgttgttaaa
     1681 tatcttggaa gatatacaca tagaattgcg ataagtaatg cacgtctcaa aaagtatgaa
     1741 gataataaag tgacattttc gtgcaaagat tattcagatt ataatcaaat aaaggaaatg
     1801 actttagacg acacagaatt ttttcgtaga tatatgatgc atgtacttcc accaaatttt
     1861 atgaagatcc gccattatgg atttctagga aaccgtaata aagaagaaag aataaaagct
     1921 gtacgaactg ctacaaacac gaaaaatcca ggaccattac taattgacta tgaacagatt
     1981 atttcggata tattaaaaag agacgtttcc atctgcatca aatgcggaca aaaacgccac
     2041 tatcaattgg aataatccca caaattccaa agtcaccagc ccaatgaaat cattgggtaa
     2101 gaggaccata cccaatatac agaaaaatcg tgaaaaatgg aacctttaaa tcaaatattt
     2161 cgatagaaac taaaattttt agaggataag caattatact tttatccata cagagatgtg
     2221 ttagttcaat aaaattaaaa caccaatgtt gtacaataat gtgcttcgat tggtgttttt
     2281 tatc
//
LOCUS       DBMDWL010000256         2278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_22597_length_2278_cov_1.972439, whole genome shotgun sequence.
ACCESSION   DBMDWL010000256 DBMDWL010000000
VERSION     DBMDWL010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22597_length_2278_cov_1.972439"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            335..1561
                     /locus_tag="ACZPDN_11685"
     CDS             335..1561
                     /locus_tag="ACZPDN_11685"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR1299315.1"
                     /translation="MSFLLENIQTPLSEQTAYILGGIVSACSYNEEQSAWLLPVIHNP
                     DKVTSTDLQSHQNALKKILSGMYAEFIKKTDDNYKKYMQPNKQGFVFLVSSATIKTPE
                     NLIETFSEHIMIASKRVRCAFITGAFDGRSSLDKHTRMLSLDERHPLCSDLIINVLKS
                     LNITSSINKSRDRITGGNPRKNQIRISAADVPEFAKTVGLISPTRVKDIENASISNFI
                     EEKDNSLSGLIKIKGLRLSKQQRKLKKVSVDKRSHEAGADQKLIDEISSTKITKPTKE
                     VSYTGKPKKKTSAVTQSQTTTFPRDKKVSLKALKIANYTCEIDAQHQTFIRKKDDLPY
                     TEPHHLVPMAYSDDFDVSLDIEENIVSLCSTCHNQVHYGKDIEIILKPLYERRKDLLA
                     RVGIYITYAELLKMYK"
     gene            1570..2253
                     /locus_tag="ACZPDN_11690"
     CDS             1570..2253
                     /locus_tag="ACZPDN_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1299316.1"
                     /translation="MISQTYLPWYKTQVKESTYLNRATTIKKHFSYFYKMETDEIEPI
                     HVQNWQLKLAKDFSPNYIRIVQGMLSIAFDRAIVLGIAKKNPSRMIGNIKSKKTKVDF
                     WTLEEFQKVISLLCKKDYYEHYLFISFWLLFMTGMRIGEASALHWSDIDFETGLLSIT
                     KTLYYRTMTDYKFVEPKTQASIRTIYIDSDTLNELKAWQSVQQQVLPDCPLVLSYNGT
                     PTSKTRDCN"
BASE COUNT          803 a          416 c          356 g          703 t
ORIGIN      
        1 ggcggtctta tcattttcag ataaatctct tatcaatttt gatgattgtc ctctgttgaa
       61 ctctgctata ttaaggtgtt ccattgggct tttcactctt ttatcgctca attacttgtc
      121 ctccatgaaa ttacacataa ttatacttat tacattatat acaaaagtaa ttacttttac
      181 aattcttttt acaatattta aatcatgttt atttacaatc ttaattacaa ttacttttat
      241 aatgatataa tattcttctt tttgtacagt ttttgatttt atatgttata attaatatag
      301 atttttatac tatatttaaa aggagcgtga tattatgagt tttctacttg aaaacatcca
      361 aacaccctta tctgaacaga cagcttatat attaggggga attgtatccg cctgttctta
      421 taatgaagaa caatccgcct ggttattgcc ggtcattcac aatcccgaca aagtaacatc
      481 tactgattta caatcacatc agaacgctct caaaaaaata ctttctggta tgtatgcaga
      541 gtttataaaa aaaacagatg ataattacaa aaaatacatg cagccaaata agcaaggctt
      601 tgttttttta gtcagttctg ccacaataaa aacgcctgaa aatctcatag aaactttctc
      661 tgaacacatt atgatagctt caaaaagagt acgatgtgct tttattactg gagcattcga
      721 tgggcgtagt tctttggaca aacatacccg aatgctttct ctggacgaaa gacatcctct
      781 ttgttcagat ctaatcatta acgtattaaa aagtctgaat ataacctctt caattaacaa
      841 gtcaagggat cgcatcacag gaggaaatcc acgaaaaaat caaatccgta tttctgcagc
      901 tgatgtacct gaatttgcta aaacagtggg acttatttct ccaaccagag taaaagatat
      961 agaaaatgcc agtattagta atttcatcga agagaaagac aatagcttaa gcggactcat
     1021 taaaataaaa ggacttcgtt tatctaaaca acagcgcaaa ttaaaaaaag taagtgttga
     1081 taaacgttct cacgaagcag gagccgacca aaaattaatt gatgaaattt catcaactaa
     1141 aatcacaaag ccaaccaaag aagtctctta tactggtaaa ccaaagaaaa aaacttctgc
     1201 tgttactcaa tctcaaacaa caactttccc acgagacaaa aaggtttcac taaaagcctt
     1261 gaaaattgca aattacacat gtgagattga cgcacagcac cagaccttta taagaaaaaa
     1321 agatgatttg ccatatactg aaccgcacca tcttgttccg atggcatatt ctgacgattt
     1381 tgatgtttct ttagatatcg aagaaaacat cgtatcgctc tgtagtacat gtcataacca
     1441 ggtgcattac ggaaaggata tcgaaataat acttaagcca ttatatgagc gtcgaaaaga
     1501 tcttcttgca cgtgttggta tttacattac ttatgccgaa ctgttaaaaa tgtataaata
     1561 atagcacgca tgatatctca gacctacctg ccgtggtata aaacacaggt aaaagaaagc
     1621 acttatctta atcgtgccac taccatcaag aaacattttt catatttcta taaaatggaa
     1681 acagacgaaa tcgaacctat ccatgttcaa aactggcagt tgaaacttgc aaaagatttt
     1741 agccctaatt acattcgtat cgtacaggga atgctttcta tcgcatttga cagagctatt
     1801 gttcttggaa ttgcgaagaa aaatccttct cgtatgatag gcaatatcaa aagcaagaaa
     1861 accaaagttg atttctggac attagaagaa tttcaaaagg ttatttcctt actctgtaag
     1921 aaagactatt atgaacatta tctgtttata tcgttctggt tattatttat gactggtatg
     1981 cgaattggcg aagcgtcagc attacattgg tcagacatag attttgaaac aggcttgctt
     2041 agcattacca aaacattata ttaccgaaca atgacggact ataaattcgt tgagccgaaa
     2101 acacaggcaa gtatccgtac tatttatatt gattcagata cactcaatga gctaaaggct
     2161 tggcaaagtg ttcagcaaca ggttcttccc gattgtccac ttgtactaag ttataacgga
     2221 actccaacca gtaaaacaag agactgtaat taattctgtg taaactctaa aagagcta
//
LOCUS       DBMDWL010000257         2248 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_23019_length_2248_cov_3.110852, whole genome shotgun sequence.
ACCESSION   DBMDWL010000257 DBMDWL010000000
VERSION     DBMDWL010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23019_length_2248_cov_3.110852"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="ACZPDN_11695"
     CDS             <1..361
                     /locus_tag="ACZPDN_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1299317.1"
                     /translation="SKKAAQFSYEADSTYYFMDLETYDTYELTADQVDFNKYFIVEGA
                     EVSLMFFEGELLSVSVPEKVELTVAETDPAIKGAPSNQTKDATTDTGLTLRVPQFIEP
                     GEKIVVFSTDGKYAGRA"
     gene            382..1128
                     /locus_tag="ACZPDN_11700"
     CDS             382..1128
                     /locus_tag="ACZPDN_11700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1299318.1"
                     /translation="MNKVVLITGGARGIGSAIALELAKHGYDIVINYLTSKQPAKELK
                     EKIESQYHVHCLTICADVSDVDQVNIMVSEIEEKMGGVDILINNAAVDLSNLFHLKNA
                     EEFKRTLDVNVVGAFNCSKRVYKHMLDQEYGRIINISSTNGINTYYPMCIDYDASKAA
                     LISMTHNLAAEFGPYINVNCVAPGFIGTDNELDGYDEEFLKEETEKIMVKRYGKPEEV
                     AYLVRFLISDEANFINNSIIRIDGGQMGSC"
     gene            complement(1256..>2248)
                     /locus_tag="ACZPDN_11705"
     CDS             complement(1256..>2248)
                     /locus_tag="ACZPDN_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1299319.1"
                     /translation="PGDGDHLGDFPLSALLKYGDTMISISGAPYDSLQEMIGLCGDST
                     QSLKANGVKYEQIDLVNDDFDDDKIISRLKENNVKLVEIQRSRGYAHRLSLTISKLER
                     IIKKIREVNKDVIIMVDNCYGELVEKLEPGHIGADVVVGSLMKNLGGGIASTGGYVAG
                     NKELINMVAERLTAPGIGKDLGANFNLNNSFFKGIFMAPNAVKSALKTAVFTAYMLEK
                     LGYKNVSPAYNDERTDIIQTLELGSKENLVSFTQGIQSTSPIDSFVRVMAAPMPGYPF
                     DEVMAAGSFTQGSTIELSADAPVVEPYTLYMQGGLTLEYGKLSILLALTNMKNN"
BASE COUNT          741 a          394 c          402 g          711 t
ORIGIN      
        1 ttctaaaaaa gcagctcaat tctcatatga agcagattct acatattact tcatggattt
       61 agaaacatac gatacttatg aattaacagc tgaccaagtt gatttcaaca aatactttat
      121 tgttgaaggt gctgaagtat ctttgatgtt cttcgaagga gaattattat ctgtttctgt
      181 tccagaaaaa gttgaattga cagtagctga aacagatcca gccatcaaag gtgcaccatc
      241 taaccaaact aaagatgcaa ctacagatac tggattaaca ttaagagttc cacagttcat
      301 cgaaccaggt gaaaaaatcg ttgtattctc aacagatgga aaatatgcag gaagagctta
      361 agcgctcttc tttttttaac tatgaataaa gttgtattaa ttacaggcgg tgctcgtggt
      421 attggaagtg caatcgcatt ggaactcgca aaacatggtt atgacattgt cattaattat
      481 ttaacttcta aacagcctgc caaagaatta aaagaaaaaa ttgaatctca atatcatgtt
      541 cattgtctta ccatttgtgc tgatgtatcc gatgtagatc aagtaaatat aatggttagt
      601 gaaattgaag agaagatggg tggcgtagat atattaatta ataatgcggc tgtggatctt
      661 tccaatctat ttcacttaaa aaatgccgaa gaatttaaaa gaacattaga tgtcaatgta
      721 gtaggtgctt ttaactgtag taaaagagtc tataaacata tgttggatca agaatatgga
      781 agaatcatca atatatcaag taccaatgga attaatacgt attatccaat gtgtattgat
      841 tatgatgctt caaaagcagc cttgatttca atgacacaca atcttgcggc tgaatttgga
      901 ccctatatca atgtgaattg tgttgctcca ggctttattg gaaccgataa tgaactagat
      961 ggctatgatg aagagttctt aaaggaagaa acagaaaaaa tcatggtgaa acgttatgga
     1021 aaaccagaag aagtggccta tcttgttcgt ttcttaatta gtgatgaagc taacttcatc
     1081 aacaattcca ttattcgtat cgatggtgga cagatgggta gctgttaaaa aagtggttaa
     1141 atccctatat ttaaagtgga ttgaccactt ttattcttta ttgattgaag tatcgtttga
     1201 atcgattcgc aacgattcga caatcgtttg aaacaaaaaa gatgcactag gcatcttagt
     1261 tgtttttcat gtttgttagg gcaagaagaa tagaaagttt tccatattct aatgttaatc
     1321 caccctgcat atataaagta tagggttcaa cgactggagc atctgcactt agttcgattg
     1381 tacttccttg tgtaaagctt ccagcagcca taacttcatc aaatggatag ccaggcattg
     1441 gagcggccat aacacgaaca aatgaatcaa taggacttgt ggattgaata ccttgtgtaa
     1501 agctaacaag attttctttc gagcccagtt ctaaagtttg aatgatatcc gttctttcat
     1561 cattataagc aggagataca ttcttgtatc ctaatttttc tagcatatag gctgtaaaaa
     1621 cggctgtttt taaagcactc tttacagcat ttggtgccat gaagatacct ttaaagaaag
     1681 agttgtttaa gttaaagttt gcacccaaat ccttaccgat tccaggagct gttaagcgtt
     1741 cagctaccat attgatcaat tctttattac cagctacata tcctccagta gaggcgattc
     1801 ctccacctaa gtttttcatt aaagatccaa ccacaacatc ggcaccaatg tgtcctggct
     1861 ctaatttttc aacaagttca ccataacaat tatccaccat gataataacg tctttgttta
     1921 cttcacgaat ctttttgatg atacgctcaa gctttgaaat ggttaatgat aaacggtggg
     1981 catatcctct agatctttgg atttccacta attttacgtt gttttctttt aaacgagaaa
     2041 taatcttatc atcatcaaaa tcatcattca ctaaatcaat ttgttcatac tttacaccat
     2101 tggcttttaa ggattgtgta gaatccccac ataaaccaat catttcctgc aaagaatcgt
     2161 aaggagcacc cgaaatggaa atcattgtat ctccatattt taatagagca gacaaaggaa
     2221 agtcacccag gtgatcgccg tcccctgg
//
LOCUS       DBMDWL010000258         2219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_23439_length_2219_cov_1.856468, whole genome shotgun sequence.
ACCESSION   DBMDWL010000258 DBMDWL010000000
VERSION     DBMDWL010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23439_length_2219_cov_1.856468"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="ACZPDN_11710"
     CDS             <1..309
                     /locus_tag="ACZPDN_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR1299320.1"
                     /translation="ICNATRVRQQAILDLENVDCLIVVGDPTSNNTQKLVDIGKKAGI
                     PDVFSIQTVEDIDKKNFEPYSTIAITSGASTPTYLTNQVKDYLSQDIEKPKIRIQEIL
                     "
     gene            complement(294..947)
                     /locus_tag="ACZPDN_11715"
     CDS             complement(294..947)
                     /locus_tag="ACZPDN_11715"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: protein motif:HMM:NF016689.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIR1299321.1"
                     /translation="MIPCSNRIQEILEWVNGDCIADIGCDHAYVVCNAILNHQSQKGY
                     ACDVAQGPLDNAKKTIEENELSDFVSCRLLDGIQGLNEDVDQIIICGMGSKLIIDILS
                     KGNLHIGLRFLLSSHKDDFILREYLRDHNIHIVREKMIYDNGHYYPILDCVVKDHIQN
                     ISSAQLHFGMNMKMDSTYLSYLDFEENKYKNILSKVNKPEFLEKIEYIKEIRTQRIS"
     gene            complement(947..2176)
                     /gene="rpoD"
                     /locus_tag="ACZPDN_11720"
     CDS             complement(947..2176)
                     /gene="rpoD"
                     /locus_tag="ACZPDN_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1299322.1"
                     /translation="MANETQKLKFTSLDEAKEFLLKSKEQNADVSQKQFLDAVSALNL
                     DDDTLDDLYNWIDENMIEFIDGDELDEDDLNEDDLNDEDLGEEDSIAEEISQLEKTFA
                     NASHAKINDPVKMYLKEIGQIPLLDPKEEPIIARQIQEGEQAKEDIKNPDLSEEEKKK
                     LAKIIADGEEAKQTLISSNLRLVVSIAKKYVGRGMLFLDLIQEGNCGLIKAVEKFDYT
                     KGFKFSTYATWWIRQSITRAIADQARTIRIPVHMVETINKLTRIQRQLVQDLGRDPMP
                     EEIAEKMENISAEKVREIQKIALDPVSLETPIGEEDDSHLGDFIEDKDTLSPDDYTNN
                     QLLKDEINAVLQGLTEREEKVLRLRFGLLDGRTRTLEEVGKEFNVTRERIRQIEAKAL
                     RKLKNPNRCKRLRDFVK"
BASE COUNT          666 a          429 c          323 g          801 t
ORIGIN      
        1 atatgtaatg cgacaagagt tcgtcaacaa gctattttag atcttgaaaa tgtcgattgt
       61 ttaatcgttg ttggcgatcc tacaagcaat aacacacaaa aactagttga tattggaaaa
      121 aaggcgggta ttcctgatgt cttttccatt caaacggttg aagatattga taaaaagaat
      181 tttgaaccct attccacaat tgcgatcaca agtggtgcaa gtacccctac atatttgaca
      241 aaccaagtta aagattatct atcgcaagac atcgaaaagc caaaaatccg tattcaggaa
      301 attctctgag tacggatttc ttttatatat tcaatttttt caagaaattc aggtttattg
      361 acttttgaaa gaatattttt atatttattc tcttcaaaat ctagatagga taaatacgtt
      421 gaatccattt tcatattcat accaaaatga agttgtgcag aacttatatt ttgaatgtga
      481 tctttcacaa cacaatctaa aattggataa taatgtccat tgtcataaat cattttttcg
      541 cgaacaatat gaatattatg atcgcgtaaa tattctcgta agataaaatc atctttatgg
      601 cttgataata agaatcttaa accaatatgt aaattccctt ttgaaagaat atctatgatc
      661 aatttagatc ccataccaca aataataatt tgatccacat cctcattcaa gccttgaatg
      721 ccatctaata atcgacaaga tacaaaatca gataattcat tctcttcaat ggttttcttg
      781 gcattatcca atggaccttg tgccacatca caagcataac ctttttggga ttgatggttt
      841 aaaatggcgt tgcagacaac ataggcatga tcacagccaa tatctgcaat acaatcgcca
      901 ttgacccatt ctaatatttc ttgaatacga ttcgaacaag gtatcattat tttacaaagt
      961 ctcttaagcg tttgcagcga tttggattct ttaatttacg caatgccttc gcctcaattt
     1021 gacgaatacg ctcacgagtg acattgaatt ctttaccaac ttcttctagc gtacgagttc
     1081 ttccgtctaa taaaccaaaa cgtaagcgca atactttttc ttcacgttct gtaagtcctt
     1141 gtaaaacagc attgatttca tctttcaaca attgattgtt tgtataatca tctggagata
     1201 atgtatcttt atcctcaatg aaatcgccta agtgagagtc atcttcttct ccaattggag
     1261 tttcaagact cactggatct aaggcaatct tttgaatttc acgtaccttt tcagcagaaa
     1321 tattttccat tttttctgcg atttcttctg gcattggatc acgacctaga tcttgtacca
     1381 attgacgttg aatacgtgtt aatttattga ttgtttcaac catgtgtact ggaatacgaa
     1441 ttgtacgagc ctggtctgcg atagcacgcg taatggattg acgaatccac catgtcgcat
     1501 atgttgaaaa tttaaatcct tttgtgtaat caaatttttc aacggctttg atcaagccac
     1561 aatttccttc ttgaatcaaa tccaagaata acatgccacg acctacatat tttttggcaa
     1621 tcgatacaac taaacgaagg tttgaagaaa tcaatgtttg ttttgcttct tcaccatccg
     1681 caataatttt tgcgagtttt ttcttttctt cctcacttaa atctgggttt ttaatatctt
     1741 cttttgcctg ttccccttct tgaatctgac gcgcaataat tggctcttct tttggatcca
     1801 acaatggaat ttgaccgatt tcttttaaat acattttaac tgggtcatta attttggcat
     1861 gtgatgcatt cgcgaatgtt ttttctagtt gtgaaatttc ttctgcgatt gaatcttcct
     1921 cgcccaaatc ttcatcgttt aaatcatcct catttaaatc atcttcatcc aattcatccc
     1981 catcaatgaa ttcaatcata ttttcatcga tccagttata tagatcgtcc aatgtgtcat
     2041 catctaaatt taaagcactg actgcatcta agaattgttt ttgagaaaca tctgcatttt
     2101 gttccttact ctttagcaag aattcttttg cctcatccaa gcttgtaaac tttaattttt
     2161 gagtttcatt tgccatactt ttatccttcc tttcgatggt tgatttcgtt tctttgaat
//
LOCUS       DBMDWL010000259         2129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_24875_length_2129_cov_2.514793, whole genome shotgun sequence.
ACCESSION   DBMDWL010000259 DBMDWL010000000
VERSION     DBMDWL010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24875_length_2129_cov_2.514793"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /gene="def"
                     /locus_tag="ACZPDN_11725"
     CDS             <1..475
                     /gene="def"
                     /locus_tag="ACZPDN_11725"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1299323.1"
                     /translation="LLMKMHEFLVASQDEELSEKYDLRPAVGIAAIQLGIPKRMCAIH
                     VLDYDEEGNVTKSTDYALANPKIVSYTPKQSYLKTGEACLSVPEDVKGYVPRHAKITV
                     EGFDVLTNKDVKIVARGFLAICLQHELDHFDGVLYYDHINKEYPLVPIENAMVIE"
     gene            638..1981
                     /locus_tag="ACZPDN_11730"
     CDS             638..1981
                     /locus_tag="ACZPDN_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIR1299324.1"
                     /translation="MNNELLKLFDIKDDVVETFTVDHKESNYEIDIRFKPSRFSCPTC
                     GSTHFISKGNKKRSLVSVPVNDKPVKMITYVKRYKCIDCNASFSDVNPIAYGDWSFTR
                     TAIISILNRLKPYNATYASIARMFGVSSTRIMEIFDTFVRIKRHSLPRVLLIDEFHFS
                     RNSKYKYPAILMNFENNLIIDIVESRTHDIMSDYLFKIDLEERKKVEYICTDMSFIFK
                     PLLKTYFPNSTLLVDHFHVTRLINDQLNHTRKRIMRKYVKNKKSREYRLLKHRYKILL
                     KSKNNIDNETFKYDKIMECHVTENMILETLLSFDDELRQAYNAKEEYLIFDQVSKGEV
                     NNSNKRKELDAVIKKFKHTHVEESISVAVTLEHWKEEILNSFTWINDRRISNGPCEGK
                     NNYVKKILSNANGMSNFQRARNRILYSQNKYETYTMNEHTDRIKRIGNPRGAYKK"
BASE COUNT          798 a          322 c          335 g          674 t
ORIGIN      
        1 tttactcatg aaaatgcatg aatttttagt tgcaagccaa gatgaagaac tttctgaaaa
       61 atatgattta agacctgcag taggtattgc agctattcaa cttggtattc caaaaagaat
      121 gtgtgccatc catgttttag attatgatga agaaggaaat gtaacaaaat ctactgatta
      181 tgctttagcg aatcctaaga ttgtttctta tacacctaaa caatcttatt taaaaactgg
      241 agaagcatgt ttatcagttc ctgaagatgt aaaaggttat gttccacgtc atgctaaaat
      301 cactgttgaa ggatttgacg tacttacaaa caaagatgta aaaattgttg ctcgaggatt
      361 tttagctatt tgtttacaac atgaacttga tcattttgat ggtgttttat actatgatca
      421 tattaataaa gaatatccat tagtaccaat cgaaaatgct atggttattg aataaataaa
      481 aggttctttc gaacctttta ttggtatatt aaattatccg gtgcttagag gttcgctcta
      541 aattattagg cggagtaccc tctaaattat cgtggggatg tgaatcatcc ccttttttga
      601 tacaatttta ctgtctggtc ctgattaaat tattattatg aacaacgaat tattaaaact
      661 atttgatatc aaggatgatg ttgttgaaac atttactgta gatcataaag aatctaatta
      721 tgaaatcgat attagattca agccatctag attttcatgc cctacatgcg gcagcactca
      781 tttcattagt aaaggcaata agaaaagatc tcttgtatct gtacctgtta atgataaacc
      841 tgtaaagatg attacttatg ttaaaaggta taaatgcatt gactgtaatg cttctttttc
      901 tgatgtaaat cctattgctt atggtgattg gtcttttact agaacagcta tcatttctat
      961 tctaaacagg ttaaaacctt ataatgctac ttatgcttcc attgctagaa tgtttggtgt
     1021 ttcttctacc agaatcatgg aaatatttga tacttttgtc agaattaaaa gacatagcct
     1081 tcctagagtt ctattgattg atgaatttca tttctctaga aactccaaat ataaatatcc
     1141 tgctatcctt atgaactttg aaaacaattt gattattgat atcgttgaat ccagaacaca
     1201 tgatatcatg tctgactatc tatttaagat tgatttggaa gaaagaaaaa aggtcgaata
     1261 catatgcact gatatgagct ttatattcaa acccttacta aagacatatt ttccaaattc
     1321 gaccttgctt gttgaccatt ttcacgttac cagactaata aatgatcaac tcaatcatac
     1381 tcgaaaaaga attatgcgta aatatgttaa aaataaaaaa tccagagaat acagattatt
     1441 gaaacatcgt tacaagatac tgttaaaatc caaaaataac atagacaatg aaacgttcaa
     1501 gtatgataag ataatggaat gtcatgtaac tgaaaacatg atacttgaaa ctttactaag
     1561 ctttgatgat gaacttagac aggcctataa tgccaaagaa gaatatttga tattcgatca
     1621 agtttcaaaa ggagaagtca ataattctaa taaaagaaag gaactcgatg cagtaattaa
     1681 aaaattcaaa catacacatg tggaagaaag tataagtgtt gcagtgacgc ttgaacattg
     1741 gaaagaagaa atactaaact cttttacatg gataaatgac agacgtatat ctaatggacc
     1801 atgtgaaggt aaaaacaatt atgtaaagaa gatcttatcc aatgcaaatg gtatgtccaa
     1861 ttttcaacgt gcaagaaaca gaatcctata ttctcaaaac aaatatgaaa cttacacaat
     1921 gaatgaacat acagacagaa taaaacgtat aggaaatcct agaggagcct acaaaaaata
     1981 aaataaaaat agaaaacaaa taaaaggagg aaaaccagga ccagacatca tcctaatttt
     2041 cctcttaaaa aagcacagaa tattttagag ggaatactga ttccacatca atatcccccc
     2101 taataattta gagcgaattt cgctaaagc
//
LOCUS       DBMDWL010000260         2122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_24998_length_2122_cov_2.277091, whole genome shotgun sequence.
ACCESSION   DBMDWL010000260 DBMDWL010000000
VERSION     DBMDWL010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24998_length_2122_cov_2.277091"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="ACZPDN_11735"
     CDS             complement(<1..233)
                     /locus_tag="ACZPDN_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299325.1"
                     /translation="MKLKDKTMKEFLENNAYFVDFFNAYFFDGEKVLKPENCMELDSE
                     MNDSNMELEKHVDVIRKYNDGNLYSAFIIENQS"
     assembly_gap    328..337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            540..1139
                     /locus_tag="ACZPDN_11740"
     CDS             540..1139
                     /locus_tag="ACZPDN_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIR1299326.1"
                     /translation="MPRIQNDCINTIEFKKSKFITYLHRTQDEQEAKDFLKAIKKEHP
                     NANHHCTAMIIGNIMRSNDDGEPSQTAGHPMLDVLMHNDMQDILAVVVRYFGGIKLGT
                     GGLVRAYSSSVLQALKVATLTTVTTLQEYQIHFSYDLIGKVDHYFRTQNIEVIEQQYE
                     EDVVYHFLVQDDISKDLMELTNGKIKIQYIQDIETECVI"
     gene            1187..1444
                     /locus_tag="ACZPDN_11745"
     CDS             1187..1444
                     /locus_tag="ACZPDN_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299327.1"
                     /translation="MDSILTLIAFFVVIFIFWTLASWLLPIILPIIIVFWIISLFTQR
                     RHVHVHTYNSTQNQNEQPKSAPKHGAIDVEYTEHEDDGDEQ"
     gene            1441..>2122
                     /locus_tag="ACZPDN_11750"
     CDS             1441..>2122
                     /locus_tag="ACZPDN_11750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1299328.1"
                     /translation="MICHNSVGYDAQIKDLLIKSAFNMNEKEVDVWMKYQYNPQHMFC
                     FWQDDKITSCLQVTKRTMMFLDRQIRVSVIGMATTLPDYRQRKQFSYLLDAAISQATY
                     NDLLTITYTNMPKLFEAKSFQHISNTKEYWIGAPLCRSGNPFHIKQKAENLYPLYFQF
                     MQYFDGSILLSEDEFDQLIQYHQNLGKSIVTITNEDKQPKGFAIYSTKDKQAHVETLI
                     YFDSQAIQD"
BASE COUNT          783 a          362 c          301 g          666 t
ORIGIN      
        1 taactttgat tctcaataat gaatgcacta taaagatttc catcattgta cttacgaatc
       61 acatccacat gcttttctaa ctccatattc gaatcattca tctctgaatc aagttccata
      121 cagttttcag gtttaagaac tttttctcca tcaaagaagt aagcattaaa gaagtcgaca
      181 aaataggcat tgttttctag aaattctttc attgttttat cttttaattt catcttatta
      241 ttcctccata ttcttaaacg tttttttgga aggtttttcg agaaaaaatg catattttga
      301 taatttcaca taattcaatc tgatttcnnn nnnnnnntta taagaaaatt atgacaaaat
      361 agaataatat tctataagtg ccttaaagat aaggcaatga atatagaaat aaaccaatat
      421 tcatatctct cttttaaaaa taataataaa caaaaagaag ccattctaga caccaacact
      481 agaatggttt ctttgttact aaacttgtag aatagtactt ttttcgttac aatacaacta
      541 tgccaagaat tcaaaatgac tgtataaata caatagaatt taaaaaaagt aaatttatta
      601 cctatcttca tagaacgcaa gatgaacaag aggccaagga ttttttaaaa gctataaaaa
      661 aagaacatcc caatgccaat catcattgta cagctatgat cattggaaac attatgcgat
      721 ctaacgacga tggtgagcca agccaaaccg ctggacatcc catgttagac gttttaatgc
      781 ataatgatat gcaagatatt ctagccgttg tagtacgcta ttttggcgga atcaagcttg
      841 gaactggagg ccttgtaaga gcctatagtt caagtgtctt acaagcttta aaagtggcaa
      901 cactaacaac ggttacaacc cttcaagaat atcaaattca tttttcttat gatttgattg
      961 gaaaggttga tcattacttt agaacacaaa atattgaagt cattgaacaa caatatgaag
     1021 aagatgttgt ctatcatttt cttgttcaag acgatatttc taaagattta atggaactta
     1081 caaatggaaa aataaaaatt caatatatac aagacattga aaccgaatgc gtaatttaat
     1141 ttacgcacaa tgaatatgat atactttaga aaagcgaggt acatttatgg atagtattct
     1201 tacccttata gcatttttcg tagtcatctt tattttttgg actttagcaa gttggctact
     1261 tccaattatt ttaccaatca tcattgtctt ctggatcatt tccctattca cacaaagaag
     1321 acatgtacat gttcacactt acaatagtac acaaaaccaa aacgaacaac caaaatcagc
     1381 accaaaacat ggagctattg atgttgaata tacggaacat gaagatgatg gagatgaaca
     1441 atgatttgtc acaacagtgt tggttatgat gcacaaatca aagacttatt gattaaatct
     1501 gcctttaata tgaatgaaaa agaagttgat gtctggatga aatatcaata taatccacaa
     1561 cacatgttct gcttctggca agatgataag attacttctt gtcttcaagt cacaaaaaga
     1621 acgatgatgt ttttggatcg acaaatacgt gtatctgtga ttggtatggc tacaacttta
     1681 cctgattata gacaaagaaa acaattttct tacttattag atgccgccat ctcacaagct
     1741 acctataacg atttattaac catcacatat acaaatatgc caaaattatt tgaagccaaa
     1801 agctttcagc acatttcaaa cacaaaagaa tattggattg gagctccact atgcagaagt
     1861 ggaaacccat tccacatcaa acaaaaagca gagaatttat acccattata tttccaattt
     1921 atgcagtatt ttgatggcag tattctatta agtgaagatg aattcgatca acttattcaa
     1981 taccatcaaa atttaggaaa atccattgtc acaatcacaa atgaagataa acagccaaaa
     2041 ggatttgcga tatattccac aaaagataaa caagcccatg tcgaaacctt gatttatttc
     2101 gactctcaag ctattcaaga tc
//
LOCUS       DBMDWL010000261         2118 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_25062_length_2118_cov_2.049579, whole genome shotgun sequence.
ACCESSION   DBMDWL010000261 DBMDWL010000000
VERSION     DBMDWL010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25062_length_2118_cov_2.049579"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            251..445
                     /locus_tag="ACZPDN_11755"
     CDS             251..445
                     /locus_tag="ACZPDN_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system TacA family
                     antitoxin"
                     /protein_id="HIR1299329.1"
                     /translation="MAFKDKEKELSYIAQYQKDNYDRITVMAPRGTKDQIKKAAELKG
                     MKISGFVLDCVQKELERMRE"
     gene            535..675
                     /locus_tag="ACZPDN_11760"
     CDS             535..675
                     /locus_tag="ACZPDN_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299330.1"
                     /translation="MKRYEFTGTNELTEKAFQVYSDNPNSEKVELGTLEDVNEFLEQF
                     AD"
     gene            1192..1842
                     /locus_tag="ACZPDN_11765"
     CDS             1192..1842
                     /locus_tag="ACZPDN_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299331.1"
                     /translation="MARRKKEQELQSDDDLCMTITPNNLKTVVKDLITNYCIDAGIDE
                     SNIPPVVWLDIINTIHDTVIKPNRLLLYINMTDVSYDADKVMNAYNIYKHICLSHNQI
                     LNIKGFLDFTGINRQTLYNWNSDSQYLAGTNSSKILNSQRLDIAKLIMSDNEQSLEAM
                     LQDHKTNPMKVLPSLNHWHAWNLPGVSREKEREPMLTAQQLPRLGPVEPEGIEEKD"
BASE COUNT          808 a          305 c          425 g          580 t
ORIGIN      
        1 tataatagag ctacctttct tttgattggt gccgatcggt gttagttgtc agctgctccg
       61 gtcggctttt tgctttgttg ttttctatgg ttataatata gcatagttta ataattgcgt
      121 caatagcata gtttaataat ttgaaaatat tttttcttct tattatataa taaagaaaat
      181 aagcaaacta aattgacaag catagtttaa taatgtataa tagcattata gataagaaag
      241 gagtttaaca atggcgttca aagataaaga aaaagaatta agctatatcg cacaatatca
      301 aaaagataac tatgatagaa tcacagtgat ggcacctaga ggaacgaaag accagatcaa
      361 gaaggctgcc gaactgaagg gaatgaaaat atccgggttt gttcttgatt gcgttcagaa
      421 agaacttgaa agaatgagag aatagtttaa taatgtattg acaagaatag tttaataaat
      481 tataataata cttgtaaaga acagaaaaac agattccaga gaggagaagc gaaaatgaaa
      541 agatatgaat ttacaggaac gaacgagctt acggaaaaag cttttcaggt atacagtgat
      601 aatccgaaca gtgaaaaagt agagttggga acgttggaag atgtaaacga atttttagaa
      661 cagtttgcag attaaaacag cataaaataa atcaatggaa aggcagtcaa taacggctgt
      721 cttttttgcg cttgtgggta caatgtttcc aaaatgtata caaattgttt ccaaaatgtt
      781 tccacagata tagatttagg tttagatata gatttagatt aagatttaga attaaaaaga
      841 gatatattct tcaaacctct tcgaggttct cagaatatac ccagaaaaag tcgcaaaatc
      901 aaagcctaaa ttcccacgat gtgccacgca ttgcgatatc tcaaaaactt caagaattgc
      961 acttgtattt ttctcttttg ttgtgtatga tatatatata aacaacagaa ccgaatcccg
     1021 ctagtatgat tactggcaag ttgtcccaga tgccgtgtga gggagtaaac agctgcaaag
     1081 gataggagaa gagagcaagg acggcaagaa agaagtgtaa cacgctcaca gatggatcag
     1141 taccaagatc tatttgtggg cgttattttt ttaggtgtgg aggtgtagaa catggcaagg
     1201 agaaagaaag aacaagaatt acaatctgac gatgatttgt gcatgactat aactccgaat
     1261 aatctcaaga ctgtggttaa agacctgatc acaaattatt gcattgatgc aggtattgac
     1321 gagagtaata taccgccggt ggtatggtta gatattataa acacgataca tgatactgtc
     1381 ataaagccta atagattatt attatatatc aatatgaccg acgtatctta tgatgctgat
     1441 aaggtgatga atgcatataa tatatataaa catatatgtt tgagccataa ccagatatta
     1501 aatattaaag ggttcttaga ttttactgga ataaatagac agacattgta taactggaat
     1561 agtgatagtc agtatcttgc tggtacaaat agcagtaaaa tattaaatag tcagagatta
     1621 gatatcgcta aactgataat gtctgacaat gagcagagtt tagaagctat gttacaagac
     1681 cacaagacta acccgatgaa agtactccca tcactgaatc actggcacgc atggaatcta
     1741 ccaggagtca gcagagagaa agaacgcgag ccaatgctca cagcgcaaca gcttccaagg
     1801 ctcggaccgg tagaaccgga aggaatcgaa gaaaaagatt gatatatttt aatatttcaa
     1861 tgagcggaaa acaaaatgat aaaataaatc attgaacaca ttacgaaatt aagcgaaccc
     1921 gtaggaaaat gatgctaatg tatgtgacaa ataagtgttt gtcgtataga ttgaaaaata
     1981 cagatcaaac gggggagggg gttaaatgag aacttgaaaa agctgctact aagtcctccg
     2041 aattcccaaa aaaacaaaaa gaccacacta aaggagaatc aatatgctaa tcaaaatcac
     2101 tttaatacta ttagttat
//
LOCUS       DBMDWL010000262         2109 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_25198_length_2109_cov_3.907371, whole genome shotgun sequence.
ACCESSION   DBMDWL010000262 DBMDWL010000000
VERSION     DBMDWL010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25198_length_2109_cov_3.907371"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(305..1321)
                     /locus_tag="ACZPDN_11770"
     CDS             complement(305..1321)
                     /locus_tag="ACZPDN_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299332.1"
                     /translation="MKIIPKEHNKVVYIENGQNLALCAYLKNDQELDNDYAFNAQKNE
                     KENLFDAFVLFDLKNNKIIEKYNVIVEDFTTIGYVRISNLHDENGEDLELCHYINNDA
                     SVVPPSPWWKSHFKLLFTIGVLCFAVLTYILFPSPKPTPSPTPDPSPNPTPTIEFNNN
                     LTTNGYLSFSIPKNVKAKMYVSSNLNADISSVAWDKINDSITAKDTLYPKRDCKIFIH
                     AYSDKTSESTYTNYEFVFDMKEFLQFFLVQFAKDKELNKIAKFMLYNVNDKRPITFDI
                     GGGIEPTIITSIYDLKQIPDYISQNMEGFKIDSVVFYPYDKPIKGPTIFSKEYPSIKY
                     IRLK"
     gene            complement(1337..1987)
                     /locus_tag="ACZPDN_11775"
     CDS             complement(1337..1987)
                     /locus_tag="ACZPDN_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299333.1"
                     /translation="MKKIFLFIFLAFVCNNIICGQTLSSNIDVKKLNAQANALAKKFG
                     DCIVMVGTSGNLNGISYSTKKKVIREIGKKFYNYYEDPRLMTTTRSNGKKNKKPIHTY
                     LNNLLAQSISSNSMQMRKYEIRWDFFCLNNGNIKEWKFSRRLSDGCNLYYREITFYQT
                     YRVIDICNKNPEQRNVERTEVDKKTMPIYAIQKPNTDKVVVLLGDITRSDCKDNRK"
BASE COUNT          661 a          355 c          333 g          760 t
ORIGIN      
        1 tgcaaaggta ctacttttgg acaatcatgt atcgtttggc aacatagttt ttgggttagc
       61 atctgcacag cactccacag ggtttgtcgg gtgcgccaaa atattcgcta agaacctcaa
      121 cagaaatagg tttagacaaa tctatgattt ctttttcttt atattctgta tatactctgt
      181 cgaaatagtc agaataattg ccaacaagtg tccatgccat tatactaatg acagcaattg
      241 taatggcaaa gaatatggaa tttatactaa atgtgtttgg cctcataaat gtcaaaaact
      301 tgtattattt caatctaata tactttatgc ttggatattc ctttgagaaa atcgttggac
      361 cttttatagg tttatcatat ggataaaaaa caacagaatc tattttgaac ccctccatgt
      421 tttgagatat gtaatcaggt atttgtttta aatcataaat agaagttata atagtcggct
      481 ctataccacc acctatatca aaagtaatgg gacgtttgtc atttacatta tatagcatga
      541 attttgcaat cttattaagt tctttatctt tcgcaaattg aactaagaaa aattgcagga
      601 actctttcat gtcaaaaaca aattcataat tagtatatgt tgactccgat gttttgtctg
      661 aataagcatg aatgaatatc ttgcaatctc ttttaggata taatgtatct tttgccgtaa
      721 tagaatcatt aattttatcc caagccacag aacttatatc tgcgtttaaa tttgaggaaa
      781 catacatttt ggcttttacg tttttaggaa tactaaaact cagataccca ttagttgtaa
      841 gattattatt gaattctatt gttggagtcg gattaggtga tggatcagga gttgggcttg
      901 gagttggctt tggggatggg aataatatgt atgttaacac tgcaaaacac aacacgccga
      961 tggtaaacaa aagtttaaaa tgtgatttcc accatggtga aggaggcaca acggaagcat
     1021 cattattgat ataatggcac aattccaagt cttctccatt ttcatcatgt aaattggaaa
     1081 tacgaacata tcctatcgtt gtaaaatctt caacaataac attgtatttt tctatgattt
     1141 tattattttt taaatcgaac agcacaaaag cgtcaaacaa attttctttt tcattttttt
     1201 gcgcattgaa agcatagtcg ttgtctaact cttggtcgtt tttgaggtat gcgcataatg
     1261 ctaaattttg gccattttct atataaacga ccttattatg ctctttcggt ataattttca
     1321 taattatagt aagctattat ttgcgattat ctttacaatc agaacgagta atatctccaa
     1381 gaaggacaac aaccttatct gtattcggtt tttgtattgc atatataggc attgtctttt
     1441 tgtcaacttc agtacgttct acattccttt gctcgggatt tttgttgcaa atatcaataa
     1501 ctcgatatgt ttgataaaaa gtaatctctc tataatacaa attgcagcca tcgcttaaac
     1561 gtcttgaaaa tttccattct tttatgttcc cattattgag acaaaagaaa tcccatctga
     1621 tttcatattt gcgcatctgc atactatttg aactaattga ttgagctaaa agattattta
     1681 aatacgtgtg tattggtttt ttgttctttt tcccattgct acgtgttgtc gtcatgagac
     1741 gcggatcctc ataatagtta tagaattttt ttccgatttc acgaataacc ttcttcttcg
     1801 tagaataact tattccattc aaatttccac tagttcctac cattacgata caatctccaa
     1861 acttttttgc caaagcgttt gcctgtgcat taagtttttt aacgtcaatg ttactagaca
     1921 aagtttgtcc gcaaataata ttgttgcaaa caaaagctaa aaatataaac aaaaatatct
     1981 tcttcattgt agtctaattt aatatttaat aggcgcaccc gacaaaccct gtggagtagg
     2041 ccgtgcagat gctaacccaa aactgttgct aaacgataca tgaagaatgg tatgtctatg
     2101 actcctttt
//
LOCUS       DBMDWL010000263         2103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_25300_length_2103_cov_1.983017, whole genome shotgun sequence.
ACCESSION   DBMDWL010000263 DBMDWL010000000
VERSION     DBMDWL010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25300_length_2103_cov_1.983017"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="ACZPDN_11780"
     CDS             complement(<1..1029)
                     /locus_tag="ACZPDN_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR1299334.1"
                     /translation="MSIFIVTFAFTLAISSKWAYSYFGLSSFEQIVYHIKVPLEGTNT
                     QFIFGWMKKCLLPGFIFGLIFSWVPTNIAKMILLLCCIYGLCQIHFFSYIFDQFKKTD
                     FYDTYFVESEVVSPEKKMNFIHIYLESMETTYAKKEDGGDANEDLLPYLSQLTKDSVS
                     FSQHEKIGGARVLTGTGWTTGGIVASTSGLPLIFSLKHKFCKDKVPFMPKVNTLGDIL
                     EKDGYNRVFMIGSDATFGGRRSYFDQHGHYDIQDTVSLKEEGILDKDYHAFWGFEDDK
                     LFEFAKEKILDLSKSSKPFAFEMLTVDTHHPYGYQSKNCKNLFKESLSNSIYHTDENL
                     KDFMEWLKK"
     gene            complement(1068..1355)
                     /locus_tag="ACZPDN_11785"
     CDS             complement(1068..1355)
                     /locus_tag="ACZPDN_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1299335.1"
                     /translation="MGKYVNKESLTQTLSIQLNMSKKDAAYAVDLIFNEMSDALANDG
                     HVDITAFGKFEIFDRKSRMGINPVTKERMMIQATKLPKFRPSQTLKNKCNK"
     gene            complement(1357..>2103)
                     /locus_tag="ACZPDN_11790"
     CDS             complement(1357..>2103)
                     /locus_tag="ACZPDN_11790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR1299336.1"
                     /translation="PICHQINDLLEKKTIIINVSKGFHPETNERMSQVIRRCIDEDKL
                     SSVVSLIGPSHAEEVVIRLLTTIDAISLKNEDALTVQNLFSNQYLRIYTGNDEIGSEL
                     GVAIKNVMAIASGILSGLGYQDNTRAALITRGLQEMIRYGVHFGGQQKTFMGLTGIGD
                     LIVTCTSVHSRNFEAGYQIGKENSVENFFKNNTKTVEGVRTAKIVHRVATENGIDMPI
                     CEEVYQILYNGKLPSDCAQDLMIRDLKKEF"
BASE COUNT          720 a          409 c          285 g          689 t
ORIGIN      
        1 cttctttagc cattccataa aatcttttag attttcatct gtatgataaa tcgaattcga
       61 taaagattct ttaaataaat tcttacaatt ttttgattga tacccataag gatgatgcgt
      121 atctacagtc aacatttcaa aggcaaaagg tttagaagat ttggataaat ctaagatctt
      181 ctctttcgca aattcaaaga gtttatcatc ttcaaacccc cagaacgcat gataatcctt
      241 atcaagaatt ccttcttctt ttaaagaaac tgtatcttga atatcataat gaccatgttg
      301 atcaaaatag cttcgtctac ctccaaatgt tgcatctgaa ccaatcataa atacacgatt
      361 atagccatct ttttctagaa tatcgccaag cgtattgact ttaggcataa atggaacttt
      421 atccttacaa aatttatgtt ttaaactaaa aatcaaagga agacctgaag tactcgcaac
      481 aataccaccc gtagtccaac ctgttcctgt taagactcta gcgccaccaa ttttctcatg
      541 ttgcgaaaaa gaaacggagt cttttgttag ctgagacaga taaggcaata aatcttcatt
      601 ggcatctcct ccatcttctt ttttggcata ggttgtttcc atcgattcta aatagatgtg
      661 aataaaattc atcttctttt caggtgaaac cacttcagat tctacaaaat atgtatcata
      721 aaaatccgtt ttcttaaatt gatcaaagat atatgaaaag aaatgaattt gacaaagacc
      781 atatatacaa caaagtaaga gaatcatttt agcaatatta gtaggaaccc aactaaaaat
      841 aaggccaaaa ataaagcctg gaagcaaaca ctttttcatc cacccaaaaa taaattgtgt
      901 gttggtacct tctaatggaa ctttaatatg gtatacaatt tgttcaaaac tagaaagtcc
      961 aaaataagaa taggcccact tactcgaaat tgccaatgtg aatgcgaatg taacaataaa
     1021 tatactcata accaatcctt tcgaaagaca aaaataggtc ataagaccta tttgttgcac
     1081 ttatttttta aagtctgact tggacgaaac ttaggaagtt ttgttgcctg aatcatcatt
     1141 ctttcttttg tcacaggatt gatacccatt cttgatttac gatcaaaaat ttcaaactta
     1201 ccaaacgccg taatatcgac atgaccatca tttgccaagg catcactcat ttcattaaaa
     1261 atcaaatcca ctgcatatgc agcatctttt ttagacatat ttaattgaat tgataaagtc
     1321 tgtgttaaag actccttatt aacatacttt cccatactaa aattccttct ttaaatcacg
     1381 aatcattaaa tcttgtgcac aatcacttgg aagttttcca ttatataaga tttggtaaac
     1441 ttcttcacaa attggcatat caatcccatt ttctgtcgca acacgatgca caatttttgc
     1501 ggtacgaaca ccttctacag tctttgtgtt gttcttaaag aagttttcaa cagaattttc
     1561 tttaccaatt tgatatccag cctcaaaatt acgagaatgt acacttgtac aagttacaat
     1621 caagtctcca attccagtaa gtcccatgaa tgttttttgt tgaccaccaa aatgaacacc
     1681 ataacgaatc atttcttgta ggccacgcgt aatcaaggct gcgcgcgtat tatcttggta
     1741 acctaaacca gacaagattc cagaagcaat agccatgacg tttttaatcg caactccaag
     1801 ttctgaacca atttcatcat tacctgtata aatacgcaag tattgatttg agaataaatt
     1861 ttgaacggta agcgcatcct cattttttaa actgatcgca tcaattgtag tcaataaacg
     1921 aataacaact tcttctgcat gacttggacc aattaaactt acaactgaac ttaatttatc
     1981 ttcatcaata caacgacgaa taacttgtga cattctttca tttgtttctg gatggaaccc
     2041 ttttgaaaca ttgatgatga ttgtcttttt ttctaacaaa tcattaattt gatgacaaat
     2101 tgg
//
LOCUS       DBMDWL010000264         2095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_25428_length_2095_cov_1.613842, whole genome shotgun sequence.
ACCESSION   DBMDWL010000264 DBMDWL010000000
VERSION     DBMDWL010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25428_length_2095_cov_1.613842"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="ACZPDN_11795"
     CDS             <1..363
                     /locus_tag="ACZPDN_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR1299337.1"
                     /translation="NPLSNKKLVKMEDLENYPRLTYEQGINNSFYFSEELHSTQPSKK
                     SIVVSDRATLFNLLIGLDGYTISSGILSTDLNGNNIVSIPLDSDEVMEIGYISDGVLS
                     NMARNYLNHLIQYIEQTK"
     gene            476..>1552
                     /locus_tag="ACZPDN_11800"
     CDS             476..>1552
                     /locus_tag="ACZPDN_11800"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR1299338.1"
                     /translation="MTVQTPYRYDVVGSFLRPEKLKQARKDYEAKKISKEELTKVEDA
                     CITDLIQKEKAAGLHVITDGEFRRATWHLDFMWGFDGVSHTPTKTGLPFHGEAAMIDD
                     TYITGKVGVNGTHPFVEHFKFVKQFEDENTIAKQTIPAPAQFLEQMILPFALENTKKY
                     YEDTEELVQDIANGYKVVIQQLYDAGCRCIQFDDCSWGMLVDEKAPLFFQTDQEGLLK
                     VQELFLRINNLAMEDKPEDLTINTHVCRGNFHSTYASSGAYDAVAKTLFAKENVHAYF
                     LEFDDARSGGFEPLKEVTSDKKVVLGLVTTKSAKLEDKETVIARIKEASQYVPLENLC
                     LSPQCGFASCEIGNKLTEEEQWAKV"
     assembly_gap    1553..1562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1563..>2095
                     /locus_tag="ACZPDN_11805"
     CDS             <1563..>2095
                     /locus_tag="ACZPDN_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR1299339.1"
                     /translation="LRPDYLKKAREEFANKTIDAKQLKAIEDKAIQELVTKEKEVGLQ
                     AVTDGEFRRRYWHLDFLADLDGVEEIKADHWSVHFKGHQPKAATLKITSKIDFGNHPF
                     VEHFKYLNSIAQGTLCKMTIPSPSMLHLICCVRSEEYTPIKRYEDMNVLYHDIAIAYQ
                     KVIRAFYDAGCRYLQLD"
BASE COUNT          747 a          324 c          391 g          623 t
ORIGIN      
        1 aatcctttat ccaataaaaa attagtgaaa atggaagatt tagaaaacta tcctcgttta
       61 acttatgaac aaggaatcaa caattcattt tacttttcag aagaattaca cagtacgcaa
      121 ccatctaaaa agagtattgt tgtttccgat cgtgcaacct tatttaattt attgattgga
      181 cttgacggat atacgatttc ttcaggtatc ttaagcacgg atttaaacgg aaacaatatt
      241 gtatccattc cactagatag tgatgaagtc atggaaatag gctatatcag tgatggagtc
      301 ttatccaaca tggcaagaaa ctatctaaat catctaatac aatatattga acagaccaag
      361 tgaaattggt cttttattat agcttttacc tataacagca taaagatttt ttcaattatt
      421 ctatactcta caaatatgat agacttaaca cacacgaaag ttgaggaatt taattatgac
      481 agttcaaaca ccttatagat atgatgtggt cggaagtttt ttaagacccg aaaaattaaa
      541 acaagcccgt aaagattatg aagccaaaaa gatttcaaaa gaagaattaa ccaaagttga
      601 agatgcatgc attacagatt taattcaaaa agaaaaggca gccggacttc atgtcattac
      661 agatggtgaa tttagacgtg ctacttggca cttggatttt atgtggggat ttgatggcgt
      721 ttctcataca ccaacaaaaa caggtcttcc tttccatggt gaagccgcta tgattgatga
      781 tacatatatt actggaaaag taggcgtgaa tggtacgcat ccttttgtag aacattttaa
      841 atttgtgaag cagtttgaag atgaaaatac gattgccaaa caaacaattc cagctcctgc
      901 acaattttta gaacagatga ttcttccttt tgcgctagaa aatacgaaaa aatattacga
      961 agatacagaa gaattggtac aagatatagc gaatggatat aaggttgtca tccaacaatt
     1021 atatgatgct ggatgtcgat gcattcaatt cgatgattgt tcatggggta tgttggtaga
     1081 tgaaaaagca ccgttgtttt tccaaacaga tcaagaaggt ttattaaagg tacaagaact
     1141 attcttgcgt attaacaatc ttgcgatgga agataaacca gaagatctaa caatcaatac
     1201 acatgtttgt cgtggtaatt tccattcaac atatgcttca agtggtgcat atgatgccgt
     1261 cgcaaaaaca ttatttgcga aagaaaatgt acatgcctat ttcttagaat ttgatgatgc
     1321 tcgttctggt gggtttgagc cattaaaaga ggtcacatcc gataaaaaag tcgtacttgg
     1381 tttagtgacg acaaaatccg ctaaattaga agacaaagaa acggtaattg ctcgtattaa
     1441 agaagcaagt caatatgtcc cattagaaaa cttgtgtcta agcccacaat gtggtttcgc
     1501 atcatgtgaa attggaaata aattaacaga agaggaacaa tgggctaaag ttnnnnnnnn
     1561 nnttttacgt cctgactatc taaaaaaagc tagagaagaa tttgcgaata aaacaattga
     1621 tgccaagcaa ttaaaagcca ttgaagataa ggccatccaa gagctagtca caaaagaaaa
     1681 agaagttgga cttcaagctg ttacagatgg agaattcaga agaagatatt ggcatttaga
     1741 ttttttagca gatctagatg gtgttgaaga aattaaggcg gatcattggt ctgtacattt
     1801 taaaggacat cagccaaagg cagctacatt aaaaattaca agtaaaattg attttggtaa
     1861 tcacccattt gtagagcact tcaaatactt aaatagtatt gcacaaggca cactttgtaa
     1921 gatgacaatc ccctctcctt ctatgcttca tttgatttgt tgcgtacgta gtgaagaata
     1981 tacaccaatc aaacgttatg aagatatgaa tgtgttatat cacgatattg cgattgctta
     2041 tcaaaaggtt attcgtgcat tttatgatgc tgggtgtaga tatctacaat tggat
//
LOCUS       DBMDWL010000265         2094 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_25441_length_2094_cov_2.318615, whole genome shotgun sequence.
ACCESSION   DBMDWL010000265 DBMDWL010000000
VERSION     DBMDWL010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25441_length_2094_cov_2.318615"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2094
                     /locus_tag="ACZPDN_11810"
     CDS             <1..>2094
                     /locus_tag="ACZPDN_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1299340.1"
                     /translation="NIPTLQNAIAQIEESEKKLPELLSQKEQVENGLQQINAEGDLNT
                     KRTLIQNAIDFIDIALKKLENYPDSSDAETIRIKLNEKKELLQGQLSLIDQAIAKKAE
                     LEAILPQIQSGIEQIQAGVAKKAELQSQLNQLLNAKNELNNAYVSLINGQAQYEDGVS
                     KIEDAKNELNKSIEQLTLSKAEFNIQKHDALRELSDAQLEIDKMEGKWIVLDRNSHYS
                     YRDYGACADRMDGIAKVFPVFFFLVAALVCMTTMTRMVDEQRNEMGTLKALGYSKLQI
                     ASKYIIYALIASILGSILGCSLGMYLFPTVIFNAWNTLYNIDQIKFLFQPGLILLASG
                     SVTGITLLATLYSIYSELIEMPSQLMRPKAAKAGKKILLERITFIWKRLSFLQKVTAR
                     NIFRYKKRFFMTIIGIAGCSALLVAGFGINDSISDIVNQQYNVIYHYDATVSAKTSEI
                     TSQIKSLKGVKDVYEEDHLAVTTKIENKDISTTVHIISNDKKFKDFCTLFNGNKEFDL
                     DDSSVLISQKMATKLNKKAGDTIKIKDANNKVIKAKIKGVFTNYVGHHIYASESLYKS
                     WNTNAKTTHIYLIKSKKTTKKFERNLGNKIMNIDGVQSVTFYSSLQKNFKDMIKSISY
                     IVVVLVISAACLAFVVLYNLSNVNISERKREIATIKVLGFTRKEVDAYINRETILLTI
                     LGSLIGLGIGIGLHH"
BASE COUNT          777 a          282 c          350 g          685 t
ORIGIN      
        1 taatatccct actttacaaa atgcgattgc tcaaattgaa gagagtgaga aaaagctacc
       61 tgaattattg agtcaaaaag aacaagttga aaatggttta caacaaatca atgccgaagg
      121 tgatttaaac acaaagagga cattaatcca aaatgctatt gattttattg atattgcact
      181 taaaaaatta gagaactatc ctgattcaag tgatgctgaa actataagga tcaaattaaa
      241 tgaaaagaaa gaactacttc aaggacaact atctttaatt gatcaagcga tagctaaaaa
      301 agcagagtta gaagcaattc tacctcaaat tcagtctgga attgagcaaa ttcaagctgg
      361 tgttgcaaaa aaggctgaat tacaatctca acttaatcaa ttattgaatg caaaaaatga
      421 attgaataat gcttatgtat cattaattaa tggtcaagct caatatgagg atggagtatc
      481 taaaattgaa gatgcgaaaa atgaattgaa taaaagtatt gagcaactta cgttatcaaa
      541 agcggaattt aatatccaaa agcatgatgc tttaagagaa ttaagtgatg ctcaattaga
      601 aatcgataaa atggaaggta agtggatcgt attagatcgc aactctcatt attcttatcg
      661 tgattatggt gcttgcgcag atcgtatgga tggcattgcc aaagtattcc ctgtattctt
      721 cttcttagta gcagccttag tttgtatgac aacaatgact agaatggttg atgaacaaag
      781 aaatgaaatg ggaactttaa aagctttagg ctattcaaag ttacaaattg cttctaagta
      841 tatcatatat gccttaatcg ctagtatttt aggatctata ttaggatgtt ctttaggaat
      901 gtatttgttc ccaaccgtca ttttcaacgc ttggaacaca ttatataata ttgatcaaat
      961 taaattttta tttcaaccag gacttatatt attagctagt ggatctgtta caggtattac
     1021 tttactggca acactttatt ctatttatag tgaattgatt gaaatgccta gtcagttgat
     1081 gcgtcctaag gctgcaaaag ctggtaaaaa gattctttta gaaagaatta catttatatg
     1141 gaaacgatta tctttcctac aaaaagtaac ggctagaaat attttccgtt ataaaaaaag
     1201 attctttatg accatcattg gtattgctgg atgctctgca ttactagttg ctggttttgg
     1261 tattaacgat agtatttcgg atattgttaa tcaacaatat aatgttattt atcattatga
     1321 tgcaacagtt tctgctaaaa cgagtgagat tacttctcaa ataaaatctt taaaaggcgt
     1381 taaagatgtt tatgaagaag atcatcttgc ggtaactaca aaaatagaaa acaaagacat
     1441 ttcaactaca gttcacatta tttccaatga caaaaaattt aaagatttct gtactttatt
     1501 taacggtaac aaagaattcg atcttgacga ttcttctgta ttgatctctc aaaaaatggc
     1561 tacaaaatta aataagaagg ctggagatac aattaaaatt aaagatgcga ataataaagt
     1621 cataaaagct aaaataaaag gtgtatttac gaattatgtt ggtcaccata tttatgcaag
     1681 tgaatcgtta tataagagtt ggaatacaaa cgcaaaaaca acgcatattt acttaattaa
     1741 atctaaaaaa acaactaaga agtttgaacg taacttaggt aataaaatta tgaatattga
     1801 tggtgtacaa agtgtaactt tctattcatc tctgcaaaaa aactttaaag atatgatcaa
     1861 gagtatttct tatattgttg ttgttcttgt tatttcagct gcttgtcttg cctttgtagt
     1921 tctgtataac cttagcaatg ttaatatttc cgaaagaaaa cgtgaaattg ctacgattaa
     1981 agtacttgga tttactagaa aagaagtaga tgcatatatc aatagagaaa caattctatt
     2041 aactatttta ggttcattga ttggtttagg tatcggtatt ggattacatc actt
//
LOCUS       DBMDWL010000266         2076 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_25738_length_2076_cov_2.622785, whole genome shotgun sequence.
ACCESSION   DBMDWL010000266 DBMDWL010000000
VERSION     DBMDWL010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25738_length_2076_cov_2.622785"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(127..708)
                     /locus_tag="ACZPDN_11815"
     CDS             complement(127..708)
                     /locus_tag="ACZPDN_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR1299341.1"
                     /translation="MNAKEEFIQIYKENIHREGADAFLEFLEGPHSDFFTAPASTRFH
                     GNMKGGLCAHSVHVYHCLKDYLDRQRVKEIYKMSYSDETIALVALLHDVCKINVYKKG
                     TRNKKIDGQWQQVDVFEFEDDIPFGHGEKSVYMIQPFMRITREEAFAIRYHMGFSGSD
                     PVNNVGKAFEMFPLAFALSTADMEATYFLDEQV"
     gene            988..1812
                     /gene="truB"
                     /locus_tag="ACZPDN_11820"
     CDS             988..1812
                     /gene="truB"
                     /locus_tag="ACZPDN_11820"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1299342.1"
                     /translation="MDGILIIDKPYGMTSHDVVNWLRKKYKQKKFGHTGTLDPNATGV
                     LVVLCGKSCKLLQFLSDTDKEYIGQIEFGKSTSTDDIWGKVEAEKEVNLDFSFSEELQ
                     KFVGKNHQLVPKTSNKKVNGKKLMDYQREGLEVPKVYSDIEIYSMEDLGDYRFKVSCS
                     SGTYVRSICRDLALNTNNLGCMKSLRRTKVGRFTIDQAQTLEDLETHEPVLYPSIYVL
                     DHLDKINYQPIEDVYQGKRIHLDNSNNEVCILDAGKTIAIYEKEREGLFKSKRGLW"
     gene            1812..>2076
                     /locus_tag="ACZPDN_11825"
     CDS             1812..>2076
                     /locus_tag="ACZPDN_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HIR1299343.1"
                     /translation="MEVIRIDYLNVPKDLLKSSCCIGFFDGLHTGHQALLDNCILKAK
                     ELGVQSGLITFDPDPWKIFFPDRVCRHITTLEDRIHLANAMGIE"
BASE COUNT          704 a          321 c          393 g          658 t
ORIGIN      
        1 agatttattg aaaaagtagt tttgtctcaa aaaaagtgcg atatctagat gtgatgcgct
       61 taaagcatac ttaataagcg aacaatcacc attcatttga aaaagagaaa ttaaaaaaac
      121 aagtccttag acttgttcat ctagaaaata tgtagcctcc atatcggcag tacttaatgc
      181 aaaggctaaa gggaacatct caaaagcttt ccccacatta ttaacaggat cacttccgga
      241 aaatcccata tgataacgaa tcgcaaacgc ttcttctcgt gttatgcgca taaatggttg
      301 aatcatgtat acactttttt caccatgacc aaaaggaatg tcatcttcaa attcaaatac
      361 atccacttgc tgccactgcc catcgatttt tttgtttctt gtaccttttt tatagacatt
      421 gatcttacat acatcatgaa gtaaggcaac aagcgcaatg gtttcatcac tatagctcat
      481 cttataaatt tcttttactc tttgtctatc tagataatcc tttaaacaat gatagacatg
      541 cacactatgc gcacatagac cacctttcat gttgccatga aaacgtgtgc ttgcaggggc
      601 agtaaaaaaa tcagagtgag gtccttctag aaattctaga aaggcatcgg caccttcacg
      661 atggatattt tctttataga tttgaataaa ttcttctttt gcgttcatac tttttaccta
      721 tttaaaaagc ctaagaacat gagatctttc cctgttaagt acaggtgggt aatctgtgaa
      781 cagcattagg atccccacgc gtagcgggtc ttcaacacaa caattcaaca tccgatttcc
      841 cttatctcga gtgtaggaaa aattaatgtc cttaggcatt tttataatag cgaacttcat
      901 aaaagaatac aagtcttgac attttagtat gaaatgtgca tggttgcaag tgcctttaaa
      961 aacaagtaga atataaaagg tgaaattatg gatggaattt taattattga taaaccgtat
     1021 ggtatgacaa gccatgatgt tgtaaattgg cttcgaaaaa aatataaaca aaaaaaattt
     1081 gggcacacag gaacattaga tcctaacgca acaggcgttt tggtggttct ttgtgggaag
     1141 tcttgtaagt tacttcagtt tttaagtgat acagataaag aatatattgg tcagattgaa
     1201 tttggaaaaa gcacttctac cgatgatatt tggggtaaag tagaagctga aaaagaggtg
     1261 aatttggatt tttctttttc agaggaacta caaaaatttg ttggaaagaa ccatcaattg
     1321 gtgccaaaaa cttcaaataa aaaagtgaat ggtaaaaaat taatggatta tcaaagagag
     1381 gggcttgaag ttcctaaagt ctatagtgat attgaaattt attcaatgga agacttaggg
     1441 gattatcgtt ttaaagtgtc ttgttcaagt ggaacgtatg ttcgtagtat ttgtcgagat
     1501 ttggctttaa acacaaataa tttaggttgt atgaaaagct taagacgaac taaagtggga
     1561 cgctttacaa ttgatcaggc acagactcta gaagatttag aaacacatga gcctgtctta
     1621 tatccttcaa tttatgtatt ggatcatttg gataaaatta attatcagcc gattgaagat
     1681 gtttatcagg gaaagcgtat tcatttagat aattccaaca atgaagtttg tatcttagat
     1741 gcaggaaaga ctatcgcaat ttatgaaaaa gagcgtgaag gcttatttaa aagtaaaaga
     1801 ggtctatggt aatggaagtg attcgtattg attatttgaa tgttccaaaa gatttattaa
     1861 aaagctcttg ttgtattggc ttcttcgatg gtttacatac tgggcatcaa gcgttgttag
     1921 acaattgtat tttgaaggca aaagaattgg gtgttcaaag tggattgatt acatttgatc
     1981 cagatccttg gaaaatcttc tttccagatc gcgtatgtag acatatcact acacttgaag
     2041 atcgtatcca tttggcaaat gcaatgggaa ttgaag
//
LOCUS       DBMDWL010000267         2033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_26532_length_2033_cov_2.747930, whole genome shotgun sequence.
ACCESSION   DBMDWL010000267 DBMDWL010000000
VERSION     DBMDWL010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26532_length_2033_cov_2.747930"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="ACZPDN_11830"
     CDS             complement(<1..456)
                     /locus_tag="ACZPDN_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299344.1"
                     /translation="MSKNLNLIIIVIMTLVFIFVPIIMKKVVWKKLLSQLDNEQYDEF
                     YKTLDSNACKFSYQAFNREYMRLSGYLAQRNDAKIEEQFDLLKNMRISNKQKASVASR
                     GFYYYLEKGKIKKAEGMLSYGKSYIDEKSFKNMQIQFSILMKKEAKYIGD"
     gene            complement(449..625)
                     /locus_tag="ACZPDN_11835"
     CDS             complement(449..625)
                     /locus_tag="ACZPDN_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299345.1"
                     /translation="MGIRHRLTDSERDDLTKDVDDLDAVYMEKLRKDGISEDLLAQFR
                     DLTLELEKDKDKDE"
     gene            complement(758..1771)
                     /gene="gap"
                     /locus_tag="ACZPDN_11840"
     CDS             complement(758..1771)
                     /gene="gap"
                     /locus_tag="ACZPDN_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004800920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR1299346.1"
                     /translation="MTVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTSPKMLAHL
                     LKYDSTQGTYAKADTVVAGENSITVDGKEITIYAEADASKLPWGELGVDVVLECTGFY
                     TSKAKAEAHIKAGARKVVISAPAGNDLPTIVYNVNHETLKPEDTVISAASCTTNCLAP
                     MANALNKLAPIKSGIMTTVHAYTGDQMTLDGPQRKGDLRRSRAAAVNIVPNSTGAAKA
                     IGLVIPELNGKLIGSAQRVPTPTGSTTILVAVVDGEVTVDQINAAMKAATTESFGYTE
                     EQLVSSDIVGMRYGSLFDATQTMAHSLGDGTTHVQVVSWYDNENSYTSQMVRTIKYFS
                     ELA"
BASE COUNT          631 a          394 c          302 g          706 t
ORIGIN      
        1 atccccaata tatttagctt ctttcttcat taaaatgctg aattgaattt gcatattctt
       61 aaatgatttt tcatcaatat agctttttcc ataagaaagc ataccctctg cttttttaat
      121 tttacctttt tctaagtaat aatagaatcc acgagaagca acacttgctt tttgtttatt
      181 tgaaatacgc atgtttttca ataaatcaaa ttgttcttca attttagcat catttctttg
      241 tgctaagtat ccacttaaac gcatatactc acgattaaat gcttgatatg aaaatttaca
      301 agcatttgaa tccaatgttt tatagaattc atcatattgt tcattatcca attgagataa
      361 taattttttc cataccactt tcttcataat gattggcaca aagataaaaa ccaatgtcat
      421 tatgacgata attattaaat ttaaattctt actcatcttt atccttatcc ttttctaatt
      481 ctaaagttaa atcacgaaac tgagccaata gatcttcact aattccgtct tttcttaatt
      541 tttccatgta gacagcatcc aaatcatcta catcttttgt caaatcatcg cgctcagaat
      601 ctgtcaaacg atgtcgaatt cccatcgcta acacctcaca atctctaaag gttagaatac
      661 agtatttacc tatttatttc aagttttggt ttgcactttt tagttaagat gaaaaaaaag
      721 gaatccaatt ggattccttt aaaaagtctt acgataatta agctaattct gagaaatact
      781 taattgtacg aaccatttgg ctagtgtaag agttttcgtt gtcgtaccaa gaaacaactt
      841 gtacgtgagt agttccgtca cctaatgagt gagccattgt ttgagtagca tcgaacaatg
      901 atccgtaacg cataccaacg atatcactag atactaattg ttcttcagta tatccgaatg
      961 attcagttgt agcagctttc atagcagcat tgatttggtc aactgtaact tctccgtcaa
     1021 caacagctac taaaatagta gttgatcctg taggagttgg aacacgttga gcagatccga
     1081 ttaattttcc gtttaattca ggaataacca atccgatagc ttttgcagca cctgttgagt
     1141 taggaacaat gttaactgca gcagcacggc tacgacgtaa atcaccttta cgttgaggtc
     1201 cgtctaatgt catttggtca ccagtgtaag cgtgaactgt agtcatgata cctgatttaa
     1261 ttggagctaa tttgttcaaa gcgttagcca ttggagctaa acagtttgtt gtacaagaag
     1321 ctgcagaaat tactgtatct tctggtttta atgtttcgtg gtttacattg taaacgattg
     1381 ttggtaagtc atttcctgct ggagcagaga taacaacttt acgagcacct gctttaatgt
     1441 gtgcttctgc tttagctttt gaagtataga atcctgtaca ttctaatact acgtcaactc
     1501 ctaattcacc ccaaggtaat ttagaagcat cagcttctgc gtagatagtg atttcttttc
     1561 catctactgt aattgagttt tcaccagcaa caactgtgtc agccttagcg taagttcctt
     1621 gagttgaatc atatttcaac aagtgagcta acatcttagg gctagtcaag tcattgatag
     1681 ctactacttc gtagccttca gcaccaaaca tttgacggaa tgccaaacgt ccgatacgtc
     1741 caaaaccgtt aatcgcaact tttactgtca tgtcagtaaa tcctccttta aataccattg
     1801 ttattataaa agcctaccgt taatttgtca attctctaac aagtaaaact tttaacaact
     1861 aacaaccact tatattttag actatttaca gtccattctc aagtattttc acacttttta
     1921 cataacctta attgtgctat tatattcaaa aggaggaatt caaaatgaac caaagtttaa
     1981 tcctagattt acttgaaaat aacgctcgtc taacttcaca agatctagcg gat
//
LOCUS       DBMDWL010000268         2025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_26690_length_2025_cov_3.816528, whole genome shotgun sequence.
ACCESSION   DBMDWL010000268 DBMDWL010000000
VERSION     DBMDWL010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26690_length_2025_cov_3.816528"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            101..472
                     /locus_tag="ACZPDN_11845"
     CDS             101..472
                     /locus_tag="ACZPDN_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299347.1"
                     /translation="MSEYCRYVIIKNNELKILVTKRMTKLIKKELGFFDYRDYLFEDN
                     LSIYLLVSDLSIFGDDTSIIYRSYPGDCDSTFSGTVIFTKMNEHGYVSLNKDDIDLIL
                     KHINKLSNGLFEMRYFNDDTC"
     gene            complement(422..1486)
                     /locus_tag="ACZPDN_11850"
     CDS             complement(422..1486)
                     /locus_tag="ACZPDN_11850"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR1299348.1"
                     /translation="MREKYELIDRTLESNKNVLTVSYLCQSAGVSRSGYYSWKKRKND
                     PLSVYNKKEEQDRKDFDLILEAYNFKGYDKGRRGIHMRLLRMGIIMNHKKISRLMDKF
                     NLFCPIRKANPIRRMAKAIKTSNYTDNILNRHFDEYGPGYVLETDITYLFYGAKHSKA
                     YLSVIKDGFTKQVLSYVLSPSLEEDFVLKTINQLFKKHKHDIHTDALIHSDQGAHYTS
                     MRFIDLLKSLEIRQSMSRRGNCWDNAPQESFFGHMKDEIGKYIEIISTYDELEKEIDN
                     YMYYYNNERYQYNLAKLSPNEYLEYYKTGNYPLKHIIEEPIEYKKKFSLVKQNLDRNL
                     TFKKLTSVIIKISHLKQTIG"
     gene            complement(1483..1911)
                     /locus_tag="ACZPDN_11855"
     CDS             complement(1483..1911)
                     /locus_tag="ACZPDN_11855"
                     /inference="COORDINATES: protein motif:HMM:NF042545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HIR1299349.1"
                     /translation="MGINYFTDEQLKELKNNPYVVKCSAKNITYAEEFKDLFWIDYQN
                     GMPPIEIFKKYGFDPYALGAKRRSNFIQRLKKQAARTDGFKDTRSTNSGRPPVKELSL
                     EEQLERLKHKNMVLQQENDFLKRVRFINKKQIVKQSKNKQ"
BASE COUNT          629 a          313 c          277 g          806 t
ORIGIN      
        1 taaagtggcc cctgtgtcaa ggacaataaa taaaaaaggt cttaaaatta aataaaaata
       61 aatatatcct ctaggtgtca aataccggag gatatatttt atgtcagaat attgcagata
      121 tgtaattata aaaaataatg aattaaaaat tttagtaaca aaaagaatga caaaacttat
      181 aaaaaaggaa cttggctttt ttgattatcg tgactatcta tttgaagata atctttctat
      241 ctatcttctt gtaagtgatc tgagcatttt tggtgacgat acttctatta tttatagatc
      301 atatccgggt gattgtgata gtacatttag cggaaccgtt atctttacca agatgaatga
      361 acatggctat gtatcattga ataaagacga tattgatttg attctaaaac atataaataa
      421 gttatccaat ggtttgtttg agatgagata ttttaatgat gacacttgtt aattttttga
      481 atgttaaatt tctgtctaaa ttttgcttta ctagactgaa ttttttctta tattcaatag
      541 gttcttctat tatatgtttt aaaggataat ttcctgtttt gtaatattca agatattcat
      601 ttggggacaa cttggcaaga ttatattgat aacgttcatt attgtagtaa tacatataat
      661 tatcaatctc tttttccaat tcatcatatg tagaaatgat ttctatatat ttacctattt
      721 catccttcat atgtccaaaa aatgattctt gaggagcatt gtcccaacaa tttcctcttc
      781 ttgacattga ttgtctgatt tctagacttt taagcagatc aataaatctc atacttgtat
      841 aatgtgcacc ttgatcacta tgaatcaaag cgtctgtatg gatatcatgt ttatgttttt
      901 taaacagttg atttattgtt tttagcacaa aatcttcctc taatgatggt gataatacat
      961 atgaaagaac ctgttttgta aatccatcct ttataacaga cagatatgct tttgaatgtt
     1021 ttgcaccata aaataaatat gtgatatccg tttccaatac atatcctgga ccatattcat
     1081 caaaatgtct gtttaagata ttatctgtat aatttgatgt ttttatcgct ttagccattc
     1141 ttctgatggg attggctttt cttattggac aaaacaggtt gaatttatcc atcaatcttg
     1201 atattttctt atgattcatt ataattccca tacgaagaag tctcatgtga ataccacgcc
     1261 tacccttgtc ataaccttta aagttataag cttctaaaat tagatcaaag tctttacgat
     1321 cttgttcttc tttcttgtta taaacagata atggatcatt ttttcttttc ttccatgaat
     1381 agtatccact tctcgaaaca ccagctgatt gacatagata tgaaacagtt aaaacatttt
     1441 tattggattc taacgttcta tcaataagtt catatttttc tctcactgtt tgttcttcga
     1501 ttgttttaca atttgctttt tgttgataaa tctcactctt tttaaaaaat cattctcctg
     1561 ttggagaacc atatttttgt gttttaatct ttctagttgc tcttctaaag ataattcttt
     1621 gacaggaggt ctaccactat ttgtacttct tgtatcttta aagccatctg ttcttgcagc
     1681 ttgtttcttt agacgttgta taaagttaga tctccgctta gcacctaatg catagggatc
     1741 aaatccgtat tttttaaata tttcaattgg cggcattcca ttttgatagt caatccaaaa
     1801 taaatcttta aactcttctg catacgttat gtttttagca gaacatttga ctacatatgg
     1861 attgtttttc aattccttta gctgttcatc tgtgaaataa tttattccca tataatcacc
     1921 atcatttctc taattatcat aatacacaaa ataaaagccc tgtaaaacag gactaggtac
     1981 taatgtactt tttttattgt gtccgtttta cagggtccat tttaa
//
LOCUS       DBMDWL010000269         1980 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_27549_length_1980_cov_2.763704, whole genome shotgun sequence.
ACCESSION   DBMDWL010000269 DBMDWL010000000
VERSION     DBMDWL010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27549_length_1980_cov_2.763704"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(114..1922)
                     /locus_tag="ACZPDN_11860"
     CDS             complement(114..1922)
                     /locus_tag="ACZPDN_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1299350.1"
                     /translation="MEIVTMVLSLLGGIALFLFGMSLMGDGLKSVAGNKLEAFLYKMT
                     NTSLKGVALGAGVTSVIQSSSATTVMVIGFVNSGMMKLRQAIGIIMGANIGTSITGWI
                     LCLSYVGGSSGIASLFSTATITTLFAFIGICLKTFGKRTTHKNVGNIMLGFAILMTGM
                     QQMSGAVSPLRTNETFISMLTMFSNPIMGILVGILFTAVLQSASAAIGILQALSMTGS
                     LTFAAAFPINLGIGIGAACPVLISALSANKNGKRTALEYLINDLFGMIIWSILFYGSN
                     LFMHYDFLNKVMSPINIALMNTAIRCGNVLVLFWFIPQIEKLLMRIVKDSPEDLAEQA
                     DFDLLEERLIPYPALAIAQCHRVMNGMAKKVRNNVNRSMNLINEFRPEKYEKVHRKET
                     LIDKYEARLGSYLMQLTKQALNTEQTRQASLYLHTISDFERIGDHAADIAEMSKSINE
                     NRMEMSEAAMDELNLVMEAVRESMNMTYHAYLEDDLDLARRVSPLGIVIRGLCDEMKL
                     RHVERLSQGNCGLEEGTVFNDLLNSLNRIATHCASSMVALVKIREKDTDIHIHDSKVY
                     AIDGDDYKTTLAEYREKYDLGQREGRIVSMEDDQVE"
BASE COUNT          663 a          405 c          279 g          633 t
ORIGIN      
        1 agatgtgtat tttcattttt tatttatgta aaagctaatc aaacaaaaag agaggagctg
       61 aactccataa gtaataaaat ttacttattc gttacaaccc ccttttttaa tttctattca
      121 acttgatcgt cttccatact tacaatacga ccttctcttt gtccaagatc atatttttcg
      181 cgatattcag ctaacgtcgt tttataatcg tcaccgtcga tcgcatatac ttttgaatcg
      241 tgaatatgga tatctgtatc tttttcacga atcttgacca aggccaccat actacttgca
      301 cagtgtgtgg caatacggtt taaactattc aataaatcat tgaatactgt accttcttca
      361 agtccacagt ttccttgaga taaacgttca acgtgacgta atttcatttc atcacatagt
      421 ccacgaatga caattccaag tggagataca cgtcgagcca aatctaaatc atcttctaga
      481 tatgcatggt atgtcatatt catcgattca cgaacggctt ccatgacaag gtttaactca
      541 tccatggcag cttctgacat ttccatacga ttttcattga tactctttga catctcggca
      601 atatctgccg cgtgatcacc gattctttca aagtcactaa tagtatgaag atataatgag
      661 gcttgtcttg tttgttcagt atttaatgct tgttttgtaa gttgcatcaa ataagaacct
      721 aaacgcgctt catatttatc aatcaatgtt tctttacgat gtactttctc atatttttca
      781 ggtctaaatt cattgatcaa gttcattgaa cgattcacat tattacgaac ctttttagcc
      841 ataccattca taacacgatg acattgagca atagctaagg caggataagg aatcaaacgt
      901 tcttctaata aatcaaagtc agcctgttct gctagatctt ctggactgtc tttgacaata
      961 cgcatcaata atttttcaat ttgaggaatg aaccagaata aaactaatac atttccacaa
     1021 cgaatcgctg tattcattaa tgcaatattg attggagaca taactttatt caaaaaatca
     1081 tagtgcataa atagatttga accatagaat aagatagacc aaataatcat accaaataag
     1141 tcattgatca aatattctaa tgcggtacgt ttaccattct tatttgcact taaagcagaa
     1201 atcaacactg gacatgcagc accaatacca attcctaagt taattgggaa ggctgccgca
     1261 aatgtcaatg agcctgtcat agatagtgcc tgcaaaatac caattgcagc acttgcactt
     1321 tgtaaaacag ctgtaaacaa aataccaaca agaataccca taattggatt tgagaacatt
     1381 gtaagcatac taatgaatgt ttcatttgtt cttaaaggtg aaacagcacc actcatttgt
     1441 tgcatacctg tcattaaaat cgcaaaacca agcatgatat ttccaacatt tttatgtgta
     1501 gtacgttttc caaatgtttt taaacaaata ccaataaacg caaacaatgt tgtgatcgta
     1561 gctgttgaaa ataagctcgc aataccacta gatccaccaa cataagataa acacaatatc
     1621 caaccggtaa tacttgtacc aatattagca cccataataa taccaattgc ttgtcttagt
     1681 ttcatcatac ctgagttaac aaaaccaatg accattactg tcgtagcact actagattga
     1741 ataacagaag taacacctgc acctagggca acacctttta gacttgtgtt tgtcatcttg
     1801 tacaagaatg cttccaactt attaccagct acgcttttta gtccatcgcc cattaaagac
     1861 ataccgaaca aaaataacgc aatgccacct aatagggata agaccattgt aactatttcc
     1921 atcttgaact ccttttataa aatgaatatt ttattttcaa ttttttcacg caaaaaattc
//
LOCUS       DBMDWL010000270         1964 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_27854_length_1964_cov_2.285561, whole genome shotgun sequence.
ACCESSION   DBMDWL010000270 DBMDWL010000000
VERSION     DBMDWL010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27854_length_1964_cov_2.285561"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..1128)
                     /locus_tag="ACZPDN_11865"
                     /pseudo
     CDS             complement(1..1128)
                     /locus_tag="ACZPDN_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019520.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
     gene            1597..1884
                     /locus_tag="ACZPDN_11870"
     CDS             1597..1884
                     /locus_tag="ACZPDN_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299351.1"
                     /translation="MKHAFNYCCIVSKDTVEITVEHKRINNALLENHNAIIKLVTKAT
                     SGYQSWERFRNGCMLILEKASILKSTRMTEALKWLKKGPEQQNVVRVQYQL"
BASE COUNT          636 a          352 c          259 g          717 t
ORIGIN      
        1 taccaaaaaa tcaatatctg catttcgttt atctaaatta ttttgaacat tcttttgtag
       61 ttcttcaatt cccaatcgaa attgaagatt actaatctgt tcatctttct tgtaaatata
      121 aataacttgg tttgtattac tcgtcgtgta cttttccggt aaattacgta ataattcctt
      181 ttctacaatt tcagtttgtc ttaactgttc actcgtgaat cttccacttt tattttttgg
      241 aagtaagcga aaagttacaa cacgaagatc atctgtatca tttaatccaa gaatattcgc
      301 aacctcgtcc tcttcttcac tcgataatgt acctgccaac aattgatact ctaagtcttt
      361 ttgatatttc ttatccaact cttcctcagc agaaagccgc atcagtgcaa actgcaaagt
      421 taaaattgca tcttccatac cagcaaaatc aatatcgttt aattgattgt ttttttcagt
      481 aatcacaaga taaaaatatc gaccatcgaa caattgcatt ttgttgatat actctacata
      541 ttctcttttt tgtatgaaat attgatgttt agtaattact tccggtatat atctttttaa
      601 ttcattttca atattaaaac ttacaggttc ttcttcactc gatgcgtaac agtcataatc
      661 actactatat attgcgactg gattgtctaa cattttatta agtagaatta atactttttc
      721 aatcgccaca tttaaattag attcattcaa caaaatgcca ctgaaaatat catgcatcaa
      781 agcgaaatat aatcggtttg ctgcctcttt attatatatt tgtcccaata tatattttat
      841 aattccaaaa taattttgat tttgagatat ttctaaaaca ggaatatgat tctcttcaca
      901 aaacttaaat aaggtatcca ataagtagga tggaattgat tcatttcgtt taataataaa
      961 tccacttatt tttttcttat ttaactcttc taaatgaaaa agaaacatct cttcgctcat
     1021 tttttcaaat acatttaaac tagttaaaag tagttctcca ccaactacat acttctttat
     1081 atctaattct tctaattctt caacaattct aattcctttt atttctctgt ctaccccccc
     1141 tcgagatttt tacctattaa tcgaatctgc aaattttcac ttgattcaag caagtctttt
     1201 acggtataca caataagcac ctcacttcca tgtttatagt tcaaccttag tcatgcgcaa
     1261 aaaacattct catgttagtc acattataat ttaacttaaa ttttctgtct agaatttagc
     1321 ttttaaaaag gcagattcat ttttttacga ctatataaaa atagttgggg tttagatatg
     1381 ctttaagatt acatagggca gtgtgctagg cagctaaaaa ttcgctccaa agtattttgg
     1441 ggctttttta gtacctgtag ctatgcaatt gttgattttc aagaggatta atatacgtta
     1501 ttatagaatt ataagattgt tgttgtacat ttatgttctt cattcagata taaatgtctt
     1561 ccaggattac atgaatgaaa ctcggattat tttgttttga aacacgcctt taattactgt
     1621 tgtatcgttt caaaagacac agtagaaatt actgtagaac acaaaagaat caacaatgca
     1681 ttgttggaga atcataacgc aatcatcaaa ttggttacga aagcaaccag tggctatcaa
     1741 agctgggagc gattcagaaa cggatgtatg ctgatcttag aaaaggcatc gattttgaaa
     1801 tcgacaagga tgacagaagc attaaaatgg ttgaaaaaag gacccgaaca acaaaatgtt
     1861 gtccgagtcc aatatcagct ataaaccgtt ttggggtata tgccgcccct taaattcttg
     1921 gagagcaaat tcacctaacc ccatccaaat ttattgcgcc tttt
//
LOCUS       DBMDWL010000271         1959 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_27954_length_1959_cov_1.663079, whole genome shotgun sequence.
ACCESSION   DBMDWL010000271 DBMDWL010000000
VERSION     DBMDWL010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27954_length_1959_cov_1.663079"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="ACZPDN_11875"
     CDS             complement(<1..207)
                     /locus_tag="ACZPDN_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299352.1"
                     /translation="MTTLTLEIENPSILTHLKAVLKAIHGVKILNVNNAASDDDVDSE
                     QPNAVTIAAMKEAKEGTDAGTVCRD"
     gene            complement(620..1162)
                     /locus_tag="ACZPDN_11880"
     CDS             complement(620..1162)
                     /locus_tag="ACZPDN_11880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1299353.1"
                     /translation="MIDFDIVRLTKDHNVLAFDCGNKDINDFLMEDAYDYQEGLLAVT
                     YLALNKSHDDVLSYFCLLNDKLAYFPGDDKKAWNKLNRKVSNHKRMKSYPAVKIGRLG
                     TQRMCSGQGLARDIIDYIKVLFTNGNRTGCRFLTVDAHRDAVGFYEKCGFSYFTDLDV
                     DEDTRLMYFDLKPFRDACGF"
     gene            complement(1174..1329)
                     /locus_tag="ACZPDN_11885"
     CDS             complement(1174..1329)
                     /locus_tag="ACZPDN_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299354.1"
                     /translation="MAKPIKETPVLTGKDAERFAQAVANPKKVEREDVLRAQAVYKRL
                     YACSELR"
BASE COUNT          593 a          405 c          306 g          655 t
ORIGIN      
        1 gtccctgcaa acagtaccag catcggtgcc ttctttagct tctttcatag cagctatggt
       61 tacagcattt ggctgttctg aatcaacgtc atcatcggat gctgcgttgt taacatttaa
      121 tatcttaact ccatgaatag cctttaacac agctttcaag tgtgttagaa tggaaggatt
      181 ctctatttcc aatgtcaatg ttgtcatatt ctaagcaaat tttattatat gcaaagatag
      241 tgcaaatcga atgcagaata tcaagcttgc ttgaatatta tgctgagatg cagcctatct
      301 tatgcaaaga taagctatat cttccatttt tacaaggatt ttgacacgaa ttttctaatg
      361 tttgggattt attccccata cagattcttc ataaacgaca tcattctcag gtcttttgca
      421 gaaggtcctt tcttgccttg caaagcaatg tcaaggtata ctttatagtc atcggagaga
      481 gacttgcgaa gttcagctgg catgagcata taccattatc ctttttcttc atcgcaatag
      541 gttactacca taaaatactt ttggagaata taaggaatct tgcgttcctt gcggacaacg
      601 taagtactgt cgtcaccttt tagaaaccac atgcatcacg aaatggttta agatcaaagt
      661 acatcaatcg tgtatcctcg tccacatcca aatctgtaaa atagctgaag ccgcatttct
      721 cgtaaaaacc taccgcatca cgatgagcat ctaccgttaa gaaacggcat cctgtacggt
      781 tgccatttgt aaacagaact tttatataat caattatgtc acgagcaaga ccttgacctg
      841 agcacattct ttgcgttcct aatctaccaa tttttacagc tggataactt ttcatgcgct
      901 tatgattgct caccttgcgg tttagtttgt tccatgcttt tttgtcatca ccagggaaat
      961 acgccaactt atcattcaac aaacaaaagt atgacaaaac atcatcatga cttttgttta
     1021 aagccagata tgtaacggcc aacaatccct cctgatagtc ataagcatct tccattaaga
     1081 aatcattaat atctttattt ccgcagtcaa acgccaacac attgtggtct tttgtcaacc
     1141 tcacaatatc aaaatcaatc atactctttt accttatctc agttcagaac aagcgtacag
     1201 tcttttatac accgcttgag cacgcaacac atcctcgcgc tccacctttt tggggttcgc
     1261 tacggcctgt gcaaagcgtt cagcatcctt tcctgtcaat acgggagttt cttttatcgg
     1321 ttttgccata atttcaaatt catttaagtt aaacattatt atttgttact atacatcgca
     1381 aaattacatc atccccccga tatttgcaaa tatttagcct tctttatttc ctctgtaagc
     1441 cacaaataca gcgtacccac tatttttcag tgggttaatt gcattagtat tttgaataaa
     1501 gtacataatt cgatgtttta aattcagcag caaagttact tctttcaaat ttaggccata
     1561 atttgagtgc ttacataaaa aaaggcggcg catatatttt ggaacataaa ttgccgtgaa
     1621 ttaaacgtaa attagcatga attatattta cactcaaaca ccctatattt acgaaaattc
     1681 acgtgacatt tacgataatt tacgttatgc gacagcaagt gttttgtaaa ccaccaatac
     1741 agcgtaccca ctatttttca gtgggttaat tgcattagta ttttgaataa agtacataat
     1801 tcgatgtttt aaattcagca gcgaagttac ttatttctaa tttaagccat aatttgagtg
     1861 cttacaaacc gaggtcaagt tacttttttt aggagcgtaa aactacatct tgctatggat
     1921 ttagctatat aaaggtacgt ttttttgtta aacaaacaa
//
LOCUS       DBMDWL010000272         1924 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_28650_length_1924_cov_1.680197, whole genome shotgun sequence.
ACCESSION   DBMDWL010000272 DBMDWL010000000
VERSION     DBMDWL010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28650_length_1924_cov_1.680197"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            277..810
                     /locus_tag="ACZPDN_11890"
     CDS             277..810
                     /locus_tag="ACZPDN_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HIR1299355.1"
                     /translation="MILKITNDFNMILNENDYQAFVASIDLLSLHCPVCGVVGLFILY
                     GHYKRFVIIDDISSGNCKIEIPIQRIQCTQCKSTHALLPTNFVPYTQFTYLFIYYIVT
                     LDENDDLITSFEVALQTIRKVKARVIEFWDSLFPDWRDFKQDDLKIESLKRHNILFGS
                     TRSYCKLCVLSPTGSQL"
     gene            862..>1924
                     /locus_tag="ACZPDN_11895"
     CDS             862..>1924
                     /locus_tag="ACZPDN_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013753.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1299356.1"
                     /translation="MIENDAFKLLLINLFHLNPDDIANIFCTTFNKMMVLIVTLADKH
                     QACPYCGFTAPKIKEYVTKEITHSALTTQKCIIKYRARRYKCPCCGKTYYEFNPFVFK
                     SQKISILTVHNILKDLKDFNETFTSVARRYHVSPTSVSTIFDNHVDIGRNPLPEIINF
                     DEVYAFKSEKSKYVCVLLDFKKQIPVDVLPNRKKEYLLNYFQKIPLEERKKVKYACSD
                     MYEVYRDVVHKVFPEAKHLLDYFHLSQDFHKKMNEVRIKVMKGYKDNKASDEYYLLKK
                     FNWLLFKNPEDEDKHGRLFDVERERKYNSHFKSYMNYYDLRKKLLEINNDLTICYSLK
                     LELVDFYSKSTIDNAKVNLE"
BASE COUNT          635 a          339 c          317 g          633 t
ORIGIN      
        1 atggcttata acatttatgt gttttgcata cataaattaa ttttgtacca cattctggac
       61 aaaaggaata tataaaacta gatagggata aatcaacctt taaaagtcat agggatttta
      121 gctctgagaa agagataacc tttaaacact tgtagggtga acttttccta tctttttgtt
      181 acagtatttt tagaagggat tggacgtaaa taaaagaagg tagaaatcct tcaccgacca
      241 aagttgcgat ttctacccgt cctcttagac aataatatga tactgaaaat caccaacgac
      301 ttcaacatga ttttgaatga aaatgattat caggcttttg ttgcttcgat tgatctgctt
      361 tctttacatt gtcctgtttg tggtgtcgtt ggtcttttta tcttatatgg acactacaaa
      421 agatttgtga ttattgacga tatttccagt ggcaattgta agattgaaat tcctattcaa
      481 cgtatccaat gtactcaatg taaatccact catgccttgc ttccaactaa ttttgtccct
      541 tatactcagt tcacttatct tttcatttac tatattgtca cacttgatga gaatgatgat
      601 ttgatcactt cttttgaagt tgctctacaa acgattcgta aagttaaagc tcgtgtaatc
      661 gaattttggg attcactctt cccagactgg agagatttca aacaggatga cttgaaaata
      721 gaatctttaa agcgtcacaa tattctcttt ggatccacta gaagttattg taaactttgt
      781 gtactgtcac caacagggtc gcaactctga ttctgatttc ttctgatatt atgtcattga
      841 aatcagagag gagatgatct tatgatcgaa aacgatgctt tcaaactgct attaatcaat
      901 ctttttcacc tgaatcctga tgatattgcc aacattttct gtactacgtt caataagatg
      961 atggttctta tcgttactct tgcagataaa catcaggctt gtccttactg tgggtttact
     1021 gcgcctaaaa tcaaggagta tgtaaccaag gagatcactc attcggctct taccacacag
     1081 aaatgcataa taaaatacag ggcacggcgc tacaaatgtc catgttgtgg aaagacctac
     1141 tatgaattca atccctttgt ctttaaatcc cagaaaatat cgattcttac tgtgcataat
     1201 attctgaagg atctgaaaga cttcaacgaa acatttactt ctgttgccag aagataccat
     1261 gtttctccaa cttctgtatc cactattttt gataatcatg tggatattgg cagaaacccg
     1321 cttccggaaa tcattaattt tgatgaagta tatgccttca aatccgaaaa gagtaaatat
     1381 gtctgtgtcc tgcttgattt taaaaagcag attcccgttg atgttctgcc gaaccgtaaa
     1441 aaggaatatc ttttaaacta ttttcaaaag atccctttag aagaacggaa aaaggtaaaa
     1501 tacgcctgtt cagacatgta tgaagtatat cgagatgttg tacataaggt ctttcctgag
     1561 gcaaaacact tgttagatta tttccatctt tctcaagatt ttcacaaaaa aatgaacgaa
     1621 gtacgtatta aagtcatgaa aggatacaaa gacaacaagg cttcagatga atattatctt
     1681 ctcaaaaaat tcaactggct gttattcaaa aatccagaag atgaagataa acatggaaga
     1741 ctgtttgatg tagaacgtga acgtaaatac aactctcatt ttaaaagcta tatgaactac
     1801 tacgatttaa gaaagaaact gcttgaaatc aataatgatt taactatttg ttactcatta
     1861 aaactagaac ttgtagactt ctattcaaaa tctacaatcg acaatgcgaa agtaaatctt
     1921 gaaa
//
LOCUS       DBMDWL010000273         1901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_29110_length_1901_cov_1.400556, whole genome shotgun sequence.
ACCESSION   DBMDWL010000273 DBMDWL010000000
VERSION     DBMDWL010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29110_length_1901_cov_1.400556"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            347..787
                     /locus_tag="ACZPDN_11900"
     CDS             347..787
                     /locus_tag="ACZPDN_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIR1299357.1"
                     /translation="MVILYTSPGCASCRKAKQWLKDNQIEFIEKNIFTSLLKESEIKY
                     LLSRCENGTEDIISVRSKAFQALKKDIDDYSMKELVSLIQENPSILKRPILLSEKSLV
                     VGYDDDEITTMMPAQLRTVVDNACTDACPNYSVCGKCREQASMN"
     gene            899..>1901
                     /locus_tag="ACZPDN_11905"
     CDS             899..>1901
                     /locus_tag="ACZPDN_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1299358.1"
                     /translation="MDYISCESAAKKMGVSARRIQQMCKQKEIVGAIKDGRNWLIPDN
                     AILSPKKPLPIGVSDFKSATTNYYYVDKTLLIRDFLNAIPMVSLFTRPRRFGKTLNMD
                     MLRVFFEKTSDNTSIYFKDKYIWQCGDYYTKHQGQYPVIFLSFKDVKCSSWQETFQKI
                     SKLISLEFMRHDELESSFVLSSYEKEQYHRFASENINEVDCQMGLQLLSLLLHKHYNK
                     ECVIIVDEYDTPIQQGHLCDFYNEIVDFMRNFFSGGLKDNPHLAFGFLTGILRVAKES
                     IFSGMNNLKTNSILDNSYSSYFGFTNEEVKVMLAYYEYEDKYQEILEWYDGYRFGNTE
                     "
BASE COUNT          661 a          245 c          350 g          645 t
ORIGIN      
        1 atacagtttc atagaagcag ccatccttta caactattat atcataatta caacacatac
       61 aactgtaaaa aataaaaatt tgacgaaaaa aaagccgtac ataaacgaca tttggctatt
      121 tcggttatga gcaagtgtta aactcccgta tcaattaaat ctgaaattgt ttcaaaataa
      181 tcatatgcga tatttgtatc taatgttttt aaattaattc tagatagaat agctttttcc
      241 attttttgac ctcactagaa aagaatatag cacatataaa taatagatac aactatttct
      301 tttaaaccta gtgatatgat ataattagtt gaaggaggca attattatgg taattttata
      361 tacttctcca ggttgtgcga gttgtcgtaa ggcaaaacaa tggttaaaag acaaccaaat
      421 tgaatttatt gaaaaaaata tttttacttc attattgaag gaaagtgaaa ttaaatattt
      481 attgtcgcga tgtgaaaatg gaacggaaga tattatttcg gttcgttcaa aagcatttca
      541 ggctttaaaa aaggatattg atgattattc gatgaaagaa cttgtatctt tgattcagga
      601 aaatccgtcg attttaaaaa gaccgattct tttatctgaa aaaagtcttg tggttggata
      661 tgatgatgat gagatcacga caatgatgcc agctcaatta agaacggttg tcgacaatgc
      721 atgtacagat gcttgtccaa attattcagt ttgtggtaag tgtagagaac aggcaagtat
      781 gaactagagg aatctagttc ttttttttcg ttttcaaacg aaacacaaac gaaatgcagt
      841 tgatgtattt cgtttaaaca gatatactta gaatataaag atgtttagga ggtgccttat
      901 ggattatata tcttgtgaat ctgctgctaa aaagatgggc gtatcagctc gtagaattca
      961 acagatgtgt aaacaaaaag aaatagttgg cgcgataaag gatggacgca attggctgat
     1021 tcctgataat gcgattttaa gtccaaaaaa gccattacca ataggtgttt ctgattttaa
     1081 gtcggcaact acgaattatt actatgtaga taagacttta ttgattcgtg attttttgaa
     1141 tgcgataccg atggtgtctc ttttcacgcg tcctaggcgt tttgggaaaa cattaaatat
     1201 ggatatgcta cgtgtgtttt ttgaaaaaac atcggataat acatctattt attttaaaga
     1261 taagtatata tggcagtgtg gggattacta tacaaaacat caaggtcaat atcctgtgat
     1321 attcttgtct tttaaagatg ttaaatgttc gagttggcaa gagacttttc aaaaaattag
     1381 taaattgatt tctctagaat ttatgcgtca tgatgaattg gaaagtagtt ttgtactaag
     1441 ctcatacgaa aaagaacagt atcatcgttt tgcgagtgaa aatataaatg aggttgattg
     1501 tcaaatgggg cttcaacttt tatctttatt acttcacaaa cattataata aagagtgtgt
     1561 cattattgtt gatgaatatg atacgcccat tcaacaaggt catctttgtg atttttataa
     1621 tgaaattgtt gattttatgc gcaatttttt ttcgggtggt ctaaaagaca atccacattt
     1681 ggcttttgga tttttaactg gtattttaag ggtcgcaaaa gaaagtatct ttagcggaat
     1741 gaacaattta aaaacgaact ccatattaga taatagttat agttcttatt ttggtttcac
     1801 aaatgaagaa gtcaaagtca tgctcgcata ttatgaatat gaagataaat accaagaaat
     1861 cttggaatgg tatgatggat atcgttttgg aaataccgag a
//
LOCUS       DBMDWL010000274         1898 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_29168_length_1898_cov_2.626600, whole genome shotgun sequence.
ACCESSION   DBMDWL010000274 DBMDWL010000000
VERSION     DBMDWL010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29168_length_1898_cov_2.626600"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            127..1791
                     /locus_tag="ACZPDN_11910"
     CDS             127..1791
                     /locus_tag="ACZPDN_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1299359.1"
                     /translation="MAAAMMTGCASGSSGTNGKTLTVGLNTGFNGIFSPLYYQTVYDD
                     YVIEMVYQSMLSYDRDNQLQPELATEQPTISEDGSTLTFKLKKGVKFSDGSKLDADDV
                     VLTFTAMADPSYTGRFSTYVDYLEGYKEYHEGDAKTLSGVEKIDDYTVAFHLATPRID
                     AISQVGTFPIISNSQFKKYKKGDTKMFEDKASEPIGTGPYVLKKFQKDSAATFVKNDK
                     FKAKDGEYQIDNVVMKICDTSTELQELQKGTVDLLPQADNADKVGTASLDKNLTYNNY
                     ASSSMQYFIYNCEAGATADPAVRQALTYAIDRQSFVDSYYSFSKGSKDVKKTEPGFVP
                     VLMANPISSQLGDIVTGKEKDDNMTYYDYDIEKAKQILDDAGWTVGSDGKREKDGQKL
                     TVRMVSTKGKDQLDTMLPMLEKAWGEELGVDFKTNLSEFNTMVATVQGDDTVNDWEVS
                     YMGWQFGSGDDTGINLLCQTGQTDNTARLSDPDLDALLDTALHTTDQAASTAAYKEVY
                     EKVSALCPYVAINGLSYYDLYNKKIKNLDTAALYDFVKALDKATIE"
BASE COUNT          682 a          307 c          358 g          551 t
ORIGIN      
        1 ttccaccaac aaaaaagttg aaatcgtcca taaatacagg aaatttcaac tattcattta
       61 aaaattcaat tgtagttata cattttttac aaagttaagg atttaaagaa ggccggtgcc
      121 gtatgtatgg cagctgcaat gatgactggt tgtgctagtg gatcatctgg tacaaatggt
      181 aaaacattga cagtgggctt aaacacaggt ttcaatggaa ttttctctcc attgtattat
      241 caaactgtat atgatgatta cgttattgaa atggtttatc aaagtatgtt gagctatgat
      301 cgtgataatc aattacaacc agaacttgcg actgaacaac caactattag tgaggatgga
      361 tctacattaa cattcaaatt gaaaaaagga gtgaaattct ctgatggttc taaattggat
      421 gcagacgatg ttgtattgac atttacagct atggctgacc caagctatac tggtcgtttc
      481 tcaacttatg ttgattactt agaaggttat aaagaatatc acgaaggaga tgcaaaaact
      541 ctaagtggtg ttgaaaaaat cgacgattac acagttgcct tccacttggc aacaccaaga
      601 atcgatgcta tcagtcaagt tggtacattc ccaatcatct ctaattcaca attcaagaaa
      661 tacaaaaaag gtgatacaaa gatgtttgaa gacaaggcta gtgaaccaat tggtactggg
      721 ccatatgtat tgaagaaatt ccaaaaagat tcagctgcca catttgtcaa gaatgataaa
      781 tttaaagcta aagatggtga ataccagatt gataatgttg taatgaagat ttgtgatact
      841 tcaacagaac ttcaagaact tcaaaaagga actgtcgatt tattaccaca agctgataat
      901 gccgacaaag ttggtactgc aagcttagac aaaaacttaa cttacaacaa ctatgcatca
      961 agctcaatgc aatactttat ttacaactgt gaagctggag ccactgcaga tcctgctgta
     1021 cgtcaagcat taacttatgc gattgatcgt caatcatttg ttgatagcta ctactcattc
     1081 tctaaaggaa gtaaggatgt taagaaaact gaacctggtt tcgttccagt attaatggct
     1141 aacccaattt catcacaatt aggtgatatc gttactggta aagaaaaaga tgacaacatg
     1201 acttactatg attacgatat cgaaaaagct aaacaaatct tagacgatgc tggttggact
     1261 gttggaagtg atggaaaacg tgaaaaagat ggacagaaat taactgttcg tatggtttct
     1321 acaaaaggta aagatcaatt agacacaatg cttccaatgc tagaaaaagc ttggggcgaa
     1381 gaacttggcg ttgatttcaa aacaaactta agtgaattca acacaatggt tgcaacagtt
     1441 caaggtgatg atacagttaa cgattgggaa gtatcttaca tgggatggca atttggttca
     1501 ggtgatgaca ctggtatcaa cttattatgc caaactggtc aaacagataa tacagctcgt
     1561 ttatcagatc ctgatttaga tgcattatta gatactgcat tacacacaac ggatcaagct
     1621 gcaagtactg ctgcttataa agaagtttac gaaaaagttt ctgctttatg tccatatgta
     1681 gcaatcaatg gtttaagcta ctatgatcta tacaacaaaa agatcaaaaa cttagataca
     1741 gctgcattgt atgacttcgt taaagcatta gacaaggcta caattgaata acaattaaag
     1801 tagtagttac gcatgaaaaa taaaatggct taaataaacg aaatcggaaa ctctattaaa
     1861 gaatttccga ttttttctag tgttcagatg gcaatatt
//
LOCUS       DBMDWL010000275         1892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_29291_length_1892_cov_4.423786, whole genome shotgun sequence.
ACCESSION   DBMDWL010000275 DBMDWL010000000
VERSION     DBMDWL010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29291_length_1892_cov_4.423786"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            168..1790
                     /locus_tag="ACZPDN_11915"
     CDS             168..1790
                     /locus_tag="ACZPDN_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299360.1"
                     /translation="MKNIRIILLLSLSLSSNNIFAQNEEVKRTDIISSLIHFFDNLSS
                     VNDEYDSMPASEFVRIYGNENALTGGGHYFRYNGQETDMTSFVQYYAKNSIEGRNINH
                     KLDISNRLINVKPIDKASSTDQRWIVKGSLQRTNADFETSDKNEDYMIKNEPIDLVVR
                     YNGHEKEISILEINIRTPRLQKVYPVYRTQAVFLPDRNMTSTNLPASGGEWSCKMQSY
                     SSRIKEYPGFDKISETRTPLGFTYSLGEQRSSTRIGNIKMDNERITGTIAQNFLKETR
                     SYTINLIQNETNKIYSVTISQSGAVKPCKWCNFFDIDDYYDLSQISISYSLKYGIGLI
                     YKHHFEDTRFSLGGLIATNFDTYRGWEKLAQASAEASVSIGIPSTSTGLKEDLYEISH
                     ETIKPETGKYCSKLDPKNEAKKYTARSLFLIQPGVNVTNWCNFTLGIGVALSHNKYFM
                     ETAYGYTRYSFTKLDPSLPDIDDIYDYKAYYKNYYYKDPIKCHLAIRPSLDFRIPVER
                     HQYINLEVGYVLTPGYKDGASLDFAIGYTWNY"
BASE COUNT          697 a          317 c          328 g          550 t
ORIGIN      
        1 gatataaaga tagcaactat atatgaaagt ccatatgtat actactttaa tgtttctgac
       61 aatatatctt ctggaactca caaatattct atgaaaatat attatcgcat aagtgaaact
      121 gctacagcat caatagaaaa caataaaact gtaagaatta aatgagaatg aaaaatatac
      181 gaataattct gcttttgagc ttaagcctaa gcagtaataa tatatttgca caaaatgaag
      241 aagttaaacg aacggatata atatcttcgt taattcactt ctttgacaac ttgtcatctg
      301 taaatgacga gtatgattcc atgcctgcgt cagagtttgt tagaatatat ggcaatgaga
      361 atgccttgac aggtggagga cattatttta gatataatgg tcaagaaaca gatatgacct
      421 catttgtcca atactatgca aaaaacagca tcgaaggcag aaatatcaat cataagttgg
      481 acatatccaa tcgtttaatc aatgtcaagc caatagacaa agcgtcatca acagaccaac
      541 ggtggattgt aaaaggtagc ctacaacgta caaatgcgga ttttgaaacc agtgacaaaa
      601 atgaggatta catgataaaa aatgaaccga tagatttggt tgtacgatat aatggacatg
      661 aaaaagaaat ctcaattttg gaaatcaaca ttagaactcc aaggcttcag aaagtttacc
      721 cagtatatcg cactcaggca gtctttttgc ctgacagaaa tatgacttca acgaatttgc
      781 cagccagtgg aggagaatgg tcatgcaaaa tgcaatccta ttcttcaaga ataaaagaat
      841 atcctggttt tgacaagatt agtgaaacca gaacaccttt aggatttaca tattcattgg
      901 gagaacaacg ttcaagtaca agaattggaa acattaaaat ggacaatgaa cgaattacag
      961 gaactatagc ccaaaacttc ttgaaagaaa ctcgttctta tacaataaat ctcatacaaa
     1021 acgaaacaaa caagatttat tctgtaacaa tctctcaatc aggtgctgta aagccatgta
     1081 aatggtgtaa cttctttgat attgatgatt attacgatct tagccaaata agtatatcct
     1141 atagtctaaa atacggaata ggacttatat ataaacatca ttttgaggat acacgcttct
     1201 ctttaggcgg tcttattgct accaattttg acacatatcg tggatgggaa aaactagctc
     1261 aagcttctgc agaagcgagt gtttcaatag gtattccttc tacatctaca ggtttgaaag
     1321 aagaccttta tgaaattagt catgaaacca taaaaccaga aacgggaaaa tattgttcta
     1381 agttagaccc caaaaacgag gcaaagaaat atactgcccg ttctttgttt ttaatacaac
     1441 caggtgtaaa tgtaaccaat tggtgcaatt ttaccttagg tataggtgtt gctttgagtc
     1501 ataataaata ttttatggaa actgcatatg gatatacaag gtattctttt accaaattgg
     1561 atccatcact tcctgatatt gatgacatat atgattataa agcttattac aaaaattatt
     1621 attataaaga tcccataaaa tgtcacttag ctatacgacc atccttggat tttcgtatcc
     1681 cggtagaaag acatcaatat ataaatttgg aagtgggata tgtgttaaca cctggatata
     1741 aagatggagc ctcattggac tttgccattg gttatacttg gaactattaa atggcgcacc
     1801 cgacaaaccc tgtggagtgc tgtgcagatg ctaacccaaa aactatgttg ccaaacgata
     1861 catgattgtc caaaagtagt acctttgcac ta
//
LOCUS       DBMDWL010000276         1885 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_29443_length_1885_cov_2.867713, whole genome shotgun sequence.
ACCESSION   DBMDWL010000276 DBMDWL010000000
VERSION     DBMDWL010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29443_length_1885_cov_2.867713"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="ACZPDN_11920"
     CDS             complement(<1..97)
                     /locus_tag="ACZPDN_11920"
                     /inference="COORDINATES: protein motif:HMM:NF020450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR1299361.1"
                     /translation="MKNYTFPKWTELPEIDLYMDQVITYINEKLKD"
     gene            210..1055
                     /locus_tag="ACZPDN_11925"
     CDS             210..1055
                     /locus_tag="ACZPDN_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1299362.1"
                     /translation="MIHIISDSSTLYSIESAKKKGLSIVPLNITVDAQTYRDFEDITS
                     TQLLTMIEEHKIPKTSQPSLGEKIDLYNELTKDGDEVIDIAMASGLSGTYQTAMMAKN
                     SCDNPDLVHVVDSQTLCGPHRLMVDTALEMANNGATAKDIVMMIVQSRMQEESYLVPV
                     DFQFLVRGGRIKGLAATLGGALKLIPILKKGVDGMGLDKFGVSRTYKKAVNMVVEDFK
                     NNGFDSNYTFYISHAFNEELAMMFEKKINETFDGAKVVIYPLSPAFITQGGPGCVAVQ
                     AIKMA"
     gene            complement(1098..>1885)
                     /locus_tag="ACZPDN_11930"
     CDS             complement(1098..>1885)
                     /locus_tag="ACZPDN_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019892007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR1299363.1"
                     /translation="LLCTILFGSKVKPNPEYEMKLETAEKITVKKAFTAWSPFIFIFI
                     FLLSTSKLVAPVNTFLSQFASTATIYTGENPSTMTFTWINTPGVWIFLSAILGGLIQK
                     ATFRDFKVVFIATIKQMSQTIITMLCVLGCAKIMGYAGMIASIASFAIAVTGSFYPFF
                     APWIGCLGTFVTGSGTSSGVLFGAVQESAAQSLHANPYWIVALNSLGVAAGKMLSPQS
                     IAIALSSVDGQGQDSKLLSKILPYGVAFIIAMSFVAYFGQKFF"
BASE COUNT          605 a          290 c          371 g          619 t
ORIGIN      
        1 tatcttttaa tttctcattg atgtaggtga tcacttggtc catatatagg tcaatttcgg
       61 gtagttcggt ccactttgga aatgtataat ttttcataat ctgattgtaa cataaaataa
      121 attgttacac aatctagaaa ttaatactag atgtattgac ttattgttat atctatatta
      181 ttgtttatct aaagagaggt aaaaaaagaa tgattcatat tatttctgat tcgtcgactt
      241 tgtattcgat tgaatcggca aagaaaaaag ggttgtcgat tgttcctttg aatattactg
      301 ttgatgcaca aacatatcga gattttgagg atatcacgag tacacaatta ttaacgatga
      361 ttgaagagca caagattcca aaaacatcac agccaagtct aggtgaaaag atagacttat
      421 ataatgaatt gactaaagat ggagatgaag tcattgatat cgcaatggca tctggactga
      481 gtggtacgta tcaaacggct atgatggcta aaaattcttg tgacaatcca gatttagttc
      541 atgtagtaga ttcacaaaca ttatgtggac ctcatcgttt aatggtggat acggctttag
      601 aaatggctaa taatggggcg acagccaaag acatcgtgat gatgatcgta caaagtcgta
      661 tgcaggaaga gtcttattta gttcctgtag attttcaatt cttagttcgt ggtggaagaa
      721 tcaaagggtt agctgctact ttgggtggcg cattaaaatt aattccaata ttgaaaaaag
      781 gcgtagatgg catgggttta gacaaatttg gtgtaagtcg tacttataaa aaggctgtca
      841 atatggtggt agaagatttt aaaaataatg gttttgattc aaattataca ttctacattt
      901 ctcatgcgtt taatgaagaa ttggcaatga tgtttgaaaa gaaaatcaat gaaacttttg
      961 atggcgcaaa agttgtgatt tatcctttat ctcctgcatt tattacgcag ggcggtcctg
     1021 gatgtgtagc tgtacaggca ataaaaatgg cttaaggaat tttccttaag ccattttttt
     1081 tttttttctt ttcttgacta gaagaacttc tgaccaaaat aagccacaaa actcattgcg
     1141 ataatgaagg caacaccata tggtaagatt tttgataata gttttgaatc ttgtccttgt
     1201 ccatctacac tgctaagtgc aattgcgatg gattgtggtg ataacatttt accagcagca
     1261 acacctagtg aatttaatgc gacgatccaa tatggattgg catgcaagct ttgagcagca
     1321 ctttcttgta cagcaccaaa taatacacct gaacttgttc ctgagcctgt cacaaatgtt
     1381 cctaaacatc caatccatgg ggcaaagaat gggtagaaac ttccagttac agcaatcgca
     1441 aaggatgcga tacttgcgat catacccgca tatcccatga ttttagcgca tccaagtaca
     1501 catagcattg tgataatggt ttgtgacatt tgtttgattg tggcaataaa taccacttta
     1561 aaatctctaa atgtagcttt ttgaatcaat ccacctagaa ttgccgacaa gaagatccat
     1621 acacctggtg tgttgatcca agtaaatgtc attgtgcttg gattttctcc tgtataaata
     1681 gtagctgttg atgcgaattg agataagaat gtattgactg gagctactaa ttttgaagtc
     1741 gataataaga agatgaaaat aaagataaat ggtgaccaag ctgtgaatgc tttttttaca
     1801 gtgatttttt cggctgtctc taacttcatt tcatattcag gatttggttt cacttttgaa
     1861 ccaaatagaa ttgtacataa taatg
//
LOCUS       DBMDWL010000277         1880 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_29569_length_1880_cov_3.896571, whole genome shotgun sequence.
ACCESSION   DBMDWL010000277 DBMDWL010000000
VERSION     DBMDWL010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29569_length_1880_cov_3.896571"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(601..1314)
                     /locus_tag="ACZPDN_11935"
     CDS             complement(601..1314)
                     /locus_tag="ACZPDN_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024637.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1299364.1"
                     /translation="MVRYVLLKFMFCPNCGAQVAPDANFCSHCGKALRSINPANSFAT
                     LFKQEFYVEYSSVDFHNNTNYVRPHTSNRRECYSGAFCVAKNNSYYIVGPDTYALPHS
                     EDIKDVKLFNNVQLICACKRFGKWGIYNSKGRDALDNFVTFEYDEIKIYSNFDDRLDK
                     DKVLIFAVCKKGGKACVRSVNTGKVLIEPGYYDDYRIGHDYEPYRNALRYKVQGKRGD
                     SYHDIYTSDYSYSEWWVNL"
     gene            1394..1516
                     /locus_tag="ACZPDN_11940"
     CDS             1394..1516
                     /locus_tag="ACZPDN_11940"
                     /inference="COORDINATES: protein motif:HMM:NF045174.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6808 domain-containing protein"
                     /protein_id="HIR1299365.1"
                     /translation="MKKPHFSIGIQTGYGVSIINNHVRPASYLGVGLSYSIFSW"
BASE COUNT          575 a          426 c          309 g          570 t
ORIGIN      
        1 gatcgcggca atctcttctc gtgtcatcat aagatctcga ttttttgcaa aagtagcgag
       61 gatctctccc cgctacaata tgcatttcgt cggaggctta ctcaccatta gcaacaatca
      121 gatcaaacca gcaccctaca tcggcgtagg actctcctac tctctgataa gcttctgagg
      181 tttttgtccc tcatggcctg tgttttataa ttaaaccatc tctagcaaca agccagagat
      241 ggtttaattt aatgtacaac tgacatctct gccagtatgg ctctcagtga cggactatta
      301 caaataagta gagaaagtgc tacctatcga agccgttcct ttttgcgaag tctattagct
      361 cttgaagagt agtcaggcac cattttccat taagttcaac tgcaatttgt tcagaagtat
      421 aatagaaata gtttgtttcg cgaaagtctt cgactgcact attctcacat ttaatattat
      481 cgaagtaata gccgattaca atgttttccg acctgtcaac gattccacag cgtcctccag
      541 gtcttcgttt aatatagtat tgtggactta gttcaaacat aaaacaataa tattataagc
      601 ctacaaattt acccaccatt ctgaatatga ataatctgac gtgtaaatgt catggtaaga
      661 atcgcctctt ttaccctgca ctttatatct aagggcattt cgatatggtt cataatcatg
      721 accgattctg taatcatcgt aatagccagg ctctattaac actttcccag tattaactga
      781 acgaacacat gccttacctc cttttttaca aacagcaaaa atcagcacct tatctttatc
      841 aagtctatca tcaaagttgc tataaatttt tatctcgtca tattcaaacg tgacaaaatt
      901 atccaatgca tctcttcctt tactattgta gattccccat ttcccgaacc tcttacaagc
      961 acagattaat tgaacattat taaataactt cacatccttg atgtcttctg aatgaggcaa
     1021 ggcgtaggtg tctggaccaa ctatgtaata agagttgttt ttagccacac aaaatgcgcc
     1081 actatagcat tcccttctat tacttgtatg aggccgaaca taattcgtat tattatggaa
     1141 gtctacagaa ctatattcta cataaaactc ctgtttgaac aaggttgcaa aagaattggc
     1201 aggattgata ctccttagag ctttcccgca atgagaacaa aaattagcat caggagcaac
     1261 ttgcgctccg cagtttggac aaaacataaa tttcaataaa acataacgaa ccactcacct
     1321 ctttcacgtg aatagtggct tgacttatat atagcaattt ttaactccgt gtaaatttag
     1381 caaattttag tgaataaaaa aaccacactt ttcaataggc atccaaaccg gctacggcgt
     1441 cagcataatc aataaccacg tgcggccagc atcttacctc ggcgtaggcc tctcctactc
     1501 catcttcagc tggtagatac tcacttacga aagataccca gagtttccac agatttcctg
     1561 caagtaagaa gcaagaagaa caagagactg ggactcttca caagatattt tgtatctttg
     1621 cgcattgagg tctgatgcca gacttcattt gttaacactc ggtgcgacca atccgcgagg
     1681 aatggccgca cctttcgtgt ttttgtcccc catccttaga tattttgtcc cccaatcaac
     1741 aaattttctg aaaaattata tatctccgaa aattaggggt gtctttccct gaaaaaagtt
     1801 gactcgcaaa tgctaaaaac agatgcgatt caatcattca agcaaacgcg atctctactt
     1861 tgagaaagcg atgaagttgt
//
LOCUS       DBMDWL010000278         1879 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_29598_length_1879_cov_2.206412, whole genome shotgun sequence.
ACCESSION   DBMDWL010000278 DBMDWL010000000
VERSION     DBMDWL010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29598_length_1879_cov_2.206412"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..528)
                     /locus_tag="ACZPDN_11945"
     CDS             complement(100..528)
                     /locus_tag="ACZPDN_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1299366.1"
                     /translation="MKISTKGRYALRLLIDLAQHNDSGFITLKDIAARQNISKKYLEQ
                     IVPILTKSQMVQSNRGFQGGYKLSKSPDQICVYDVLVATEGNLSCVACLDSDVNTCPR
                     KEDCATLYIYEGLNQVIKTYLKGITLQDILSHEQSFDYII"
     gene            709..1269
                     /locus_tag="ACZPDN_11950"
     CDS             709..1269
                     /locus_tag="ACZPDN_11950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1299367.1"
                     /translation="MRRFSRLSKDEVKEAKKHHLVSYVWQNGGYTMFGVSILDVVKKI
                     FSLIPLDSHYIKSLQKTKFEKDVVLNEALYDKVCFVGFQIEKFKKMYLEYRFYDVGKF
                     STELCKRDVGKLEAIRYICRKKGVSIFDVMAFGDHGNDADLLASCGYGVAMKNGSAQA
                     KKAAKYISDYTNNEKGVLRFINSFFV"
     gene            complement(1247..1768)
                     /locus_tag="ACZPDN_11955"
     CDS             complement(1247..1768)
                     /locus_tag="ACZPDN_11955"
                     /inference="COORDINATES: protein motif:HMM:NF015674.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HIR1299368.1"
                     /translation="MKETKTEAISSAILCILSGLVLCIFNVSILTTITRVIGCIFLVI
                     AILFLYTYFKKRNSTTLTTLILSIFLLSAGLYMSLDPKKFISILPMLVGILLIINSLS
                     HFQKVLLLKDNGFEQWKVNLAGAIFILVIGVVLLMKPIQSLDFIFSLTGSFLILNGIL
                     IFLDQYFIQKTNL"
BASE COUNT          631 a          243 c          349 g          656 t
ORIGIN      
        1 aatattgcca tctgaacact agaaaaaatc ggaaattctt taatagagtt tccgatttcg
       61 tttatttaag ccattttatt tttcatgcgt aactactact tagattatat aatcaaagct
      121 ttgttcgtga cttaaaatgt cttggagtgt gattcctttt aaataggttt taatcacttg
      181 atttaatcct tcataaatat aaagtgttgc acaatcttct tttctaggac atgtatttac
      241 atctgaatct aggcaggcaa cgcaagataa atttccttcg gttgcgacca atacatcata
      301 tacacagatt tgatctggac ttttagaaag tttgtatcca ccttgaaaac cacgattgga
      361 ttgaaccatt tggctcttgg taagaatggg tacgatttgt tccaaatatt ttttggagat
      421 attttgtctt gcggcaatgt cttttagtgt tataaaacct gaatcattat gttgagctaa
      481 atctataagt aagcgaaggg catagcgtcc tttagttgat attttcattt ttattcccct
      541 tttctttcta ttataataat atatatttag taggttttct agcaaagagg taaaaatggt
      601 taaaattatt gcttttgatt tagatggaac tttgtatgat aagcataaac atattgatga
      661 agatacagtt caaacggttt agaaatacat gattttttag atggagagat aaggcgtttt
      721 tcacgtctat ctaaggatga agtaaaagaa gctaagaagc atcatttagt gagctatgtt
      781 tggcaaaatg gcggatatac gatgtttggt gtttctatct tggatgtagt caaaaagata
      841 ttttcactta ttcctttgga ttcacattac ataaaatcat tacaaaaaac gaaatttgaa
      901 aaggatgttg tattaaatga agcattgtat gataaggttt gttttgtggg ttttcaaatt
      961 gaaaaattta aaaaaatgta tttagaatat cggttttatg atgtgggaaa gtttagtaca
     1021 gaactatgta aaagggatgt gggtaagctt gaagccattc gatatatatg taggaagaag
     1081 ggtgtatcaa tctttgatgt catggcattt ggggatcatg gaaatgatgc ggatttacta
     1141 gcctcttgtg gatatggagt tgcgatgaaa aatgggagtg cacaggcaaa aaaagccgct
     1201 aaatatatta gcgactatac aaataatgaa aagggtgtac ttcgatttat aaattcgttt
     1261 tttgtataaa atattgatcc aaaaagataa ggattccatt tagtataagg aaagatccag
     1321 ttaaagagaa aataaagtct aaggattgaa tgggtttcat aagtaataca acaccaatga
     1381 ctagaatgaa tatagctcct gcaagattga ctttccattg ttcaaatcca ttgtctttta
     1441 agagtaatac tttttgaaaa tgggataggg aattaataat caatagaatt cctacaagca
     1501 taggtaaaat actgataaat ttcttagggt ctaaactcat atataagcct gcagataata
     1561 ggaatatact taatataagt gttgttagtg ttgtactatt tcttttcttg aaatatgtat
     1621 aaagaaatag aatcgcaatg actaaaaaga tacatccaat aactcgcgta attgttgtta
     1681 atatggagac gttaaatatg cataagacta agccggacaa aatgcataga attgccgatg
     1741 agatggcttc tgtttttgtt tctttcatat aatcacaacc tggtatattg taattgaccc
     1801 atctaactgg tcgactacaa tatacttctc ctttctttca acctttaata accgtggttg
     1861 aaggcggtgt tgttaattt
//
LOCUS       DBMDWL010000279         1853 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_30146_length_1853_cov_1.683219, whole genome shotgun sequence.
ACCESSION   DBMDWL010000279 DBMDWL010000000
VERSION     DBMDWL010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30146_length_1853_cov_1.683219"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="ACZPDN_11960"
     CDS             <1..438
                     /locus_tag="ACZPDN_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HIR1299369.1"
                     /translation="EEAYIIEPIKQYSSRTKTELEYPTKIYNCDVSLNSIRKLNNRYD
                     LTHNLENIIYNELIYMGYSLTVYDNKGKEIDFLAQKDNLKYYIQVAYSVAEDKAYERE
                     FKAFNGVSQIDKKIIITMDDIDYSTSNVQHIKFKDFLFLENLK"
     gene            complement(452..1078)
                     /locus_tag="ACZPDN_11965"
     CDS             complement(452..1078)
                     /locus_tag="ACZPDN_11965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1299370.1"
                     /translation="MSKDYLPHIFESFTRQRTSSESGVLGTGLGMPIVKKLVDLMHGT
                     ISIESEEGKGTTVVVNLPHRYIIEVQEVKEVDDKEIDLTGKHILLVEDNDLNAEIAQT
                     LLEDKGIKVTWAKDGLEAITMVKENAMDCFDCILMDIQMPRMNGFEACKVIRSLPNNR
                     SQLPIIALTANAFEEDRQDCLEAGMSDHVSKPIEIQSLLQTIESVLKK"
BASE COUNT          589 a          345 c          244 g          675 t
ORIGIN      
        1 gaagaggctt atattattga accaatcaaa caatattctt ctagaacaaa aacagaattg
       61 gaatatccta caaaaattta taattgtgat gtttcgttaa attcgattcg taaattaaac
      121 aatcgttatg atttaacgca taatctagaa aatataatat acaatgaact tatatatatg
      181 ggatatagtc ttaccgtcta tgacaataag ggaaaagaaa ttgattttct agctcaaaaa
      241 gacaatctaa agtattatat ccaagtcgca tatagtgttg ccgaagataa agcatatgag
      301 cgtgaattca aagcttttaa tggtgttagt caaatagata aaaaaatcat catcacaatg
      361 gatgatattg attattctac aagtaatgta caacatatta aatttaaaga cttcttattc
      421 ttagaaaatt taaaatagcg gactttgata gctatttttt tagtacagat tcaatggttt
      481 gtagtaaaga ttgaatttca atcggttttg aaacatgatc actcatacct gcttctaaac
      541 aatcttgacg atcttcttca aaggcattgg ccgttaaggc gataattgga agttgacttc
      601 gattatttgg caaagatcgt ataactttgc aggcttcaaa tccattcatt cttggcattt
      661 gaatatccat taatatacaa tcgaaacaat ccattgcgtt ttctttaacc atcgttatgg
      721 cttctaatcc atccttcgcc caagttactt taatcccttt gtcttcaagt aatgtttgtg
      781 caatttcggc atttaaatca ttgtcctcca caagaagaat gtgttttccc gtaagatcaa
      841 tttctttatc atccacttct ttgacttctt gtacttctat gatatatcga tgaggaagat
      901 ttacaacgac agttgtcccc ttaccttctt cactttcgat acttattgtt ccatgcatca
      961 aatcaacaag ttttttcaca attggcatcc ctagtcctgt tcctaagaca cccgattcac
     1021 tagatgttct ttgtcttgtg aatgattcaa agatatgcgg taaatagtct ttcgatatac
     1081 cgtgtttttt atttttctat actttcaaaa aactaaataa taatctattg aatttcttct
     1141 gtcccgaact cactttttcc atgatttacc ccttctaaca tatatagttg tgcatctata
     1201 ccacataaat taacttattt aacatttctt caaagtcttt ttatgtgatt tactccattt
     1261 cttcaatgta tcacgttatc atttgttaat tgtcactgat tttataggat ttacacaact
     1321 caaaacaaaa gaaacattag tatctgaatt cactaatgtt tcagttaata catttttcct
     1381 tcttaaatta ttttaatatc aaccattcgc aagtttccaa ctcaccgccc gctctctagc
     1441 atacgcaaag tgcttataaa taccttcttg tttcattaat tgcttgtatg ttccccgttg
     1501 aatgatgctt cctttatcta tcacaacaat ttgatccgcg tttctgacag tttttaatct
     1561 atgtgcaatc ctaagaaatt tatcgattca attattcgat aaattcatta aagtacttct
     1621 tttcctagaa acaaatcaat agcatttata tgtttaacac catcttgttg atcacaaaaa
     1681 cgatccaaag aaattatctg actactcctc accgcaagca gtgagggttc tgaactgaca
     1741 acattatgca gcaatcgtac attattttca aactttggat tcctgtcttt ctgttggagc
     1801 agtgaactct acaatcaagc actacattta gcacattagc gattgttttc tgt
//
LOCUS       DBMDWL010000280         1811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_31132_length_1811_cov_2.018713, whole genome shotgun sequence.
ACCESSION   DBMDWL010000280 DBMDWL010000000
VERSION     DBMDWL010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31132_length_1811_cov_2.018713"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            273..1547
                     /locus_tag="ACZPDN_11970"
     CDS             273..1547
                     /locus_tag="ACZPDN_11970"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR1299371.1"
                     /translation="MKKIIISLMLLITLFSTTVSAQIKLSVNNEKIDFKGDEPTLIND
                     RTYVPVRFLAEALGAEVDWDDTHKVVIIKNYGIDGRDTKTIHYLRLGESKMVIARYYY
                     GINDGAESIVSYYFPNDIYPIIVNDRTMLPFRYVAELLGAQVYYDPTNGIAHCVKRDL
                     SNKEYRGGDEGVHSFPILSTVIGDYFADELNKWYTDYFNTTPLKKYMYNNPTYWDGTL
                     ADTTVWGGMDHLTHPEWHEWDGQGNSPTAHKFTGNLENEVGCYEPFYRTYLPNELIYY
                     HDTVSDHKRTFYPAVNNWKPNWDGDKLSAIRGRYAENQAQIDNLGTFGGKNYTKSYNE
                     YLDLTKNWGLNINADKTGLNNLKLGGIYGRCSVRVIDEEMYREISRYVIGLWAGSPGH
                     LANLLNACNQKWQKGGIGFSIVGDNAYYNVFD"
BASE COUNT          659 a          246 c          341 g          565 t
ORIGIN      
        1 taaattcctt gctgtacttt gacattttct ttccctcttt ctgatttatt atatcagatt
       61 tttgggtgtt tgtcgactct atttatatta taccactcca actctattat aacatatttt
      121 gaacaaaaaa gatattgacg taataaacga aattgtaggt attttttgag gaggacggaa
      181 aatagaaaaa atgtcaaatt ctttcattga cttttttcga taaatattat ataatataaa
      241 agtatgtgtt caaaaataag gggatttgaa aaatgaaaaa aattataata tctttaatgt
      301 tattaataac attattttct acaacagtaa gcgcacaaat aaaattatcg gtaaacaatg
      361 agaaaataga ttttaaggga gatgaaccca cactaattaa cgacagaacc tatgttcctg
      421 ttagattttt agcagaggca ttaggggcag aggttgattg ggatgatact cataaggtag
      481 taataattaa gaattatggt attgatggaa gagacacaaa aactattcac tatttaagac
      541 ttggtgagag taagatggta atagcaagat attattatgg aattaatgat ggtgctgaaa
      601 gtattgtatc ttactatttt ccaaatgata tttaccctat tatagttaat gacagaacta
      661 tgctcccatt cagatatgta gcagaacttt taggggcaca agtatattat gatcctacaa
      721 atggtattgc tcattgtgta aagagagacc ttagtaataa agagtacaga ggtggagatg
      781 aaggtgttca ttcttttcct attctcagca ctgtaattgg tgattatttt gcagatgaac
      841 ttaataagtg gtacacagat tatttcaata caactccatt aaaaaagtat atgtacaaca
      901 acccaactta ttgggatgga acattggcag acacaacagt ttggggtgga atggaccatt
      961 taactcaccc agaatggcat gaatgggatg gacaaggtaa tagtccaact gctcataaat
     1021 tcactggcaa tttagaaaat gaagttggtt gttatgaacc tttctacaga acttatttac
     1081 caaatgagtt aatttattat catgacacag tttctgacca taaaagaaca ttttaccctg
     1141 ctgtaaacaa ttggaaaccc aattgggatg gtgataaatt atctgctatt agaggtagat
     1201 atgcagaaaa tcaggcacaa attgataacc ttggaacatt tggtggtaaa aattatacca
     1261 aatcttacaa tgaatacctt gatttaacca agaattgggg tttaaatatt aatgcagata
     1321 aaactggatt aaataacctt aaacttggtg gtatttatgg aagatgctct gtaagagtta
     1381 tagatgaaga aatgtataga gaaatttcca gatatgtaat tggtctttgg gccggttctc
     1441 ctggacacct tgctaatttg cttaatgctt gcaatcagaa atggcaaaaa ggaggcattg
     1501 gtttctctat tgtaggtgat aatgcttatt acaatgtatt tgactaatat agtcaacaat
     1561 tatcaatcag agaactgcaa aatgcagttc tctttttttg gagtggtata atataaatag
     1621 agtcgacaaa cacccaaaat ctgatataat aaatcagaaa gagggaaaga aaatgtcaaa
     1681 gtacagcaaa gaatttaaag aggaagcgta aaagctatcc gatgaaatcg gagtcaagaa
     1741 ggcagcactg caattaggat tacaatacta cacgcttgcg gattggagaa aggctcgtaa
     1801 gtatgcaaaa a
//
LOCUS       DBMDWL010000281         1806 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_31251_length_1806_cov_2.381818, whole genome shotgun sequence.
ACCESSION   DBMDWL010000281 DBMDWL010000000
VERSION     DBMDWL010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31251_length_1806_cov_2.381818"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="ACZPDN_11975"
     CDS             <1..314
                     /locus_tag="ACZPDN_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299372.1"
                     /translation="KNKELLNIYQESEKGDVKVCRALDEMFEESTNKGIQMGIKQGIE
                     QGIEQGIEQGIEQGIERGVKNTQIKIAINMLVRNNQTLEEISEIVGLDLNALRELKKS
                     I"
     regulatory      404..480
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            529..1734
                     /locus_tag="ACZPDN_11980"
     CDS             529..1734
                     /locus_tag="ACZPDN_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1299373.1"
                     /translation="MLISLSYIFLVGMFLSWICKKVGLPGLLGMIFTGILLGPYCLGL
                     IDSSLLNISSELRKIALIIILMRAGLNLDLSDLKRVGRPAILMCFVPACFEMIGMILL
                     APKLLGISLLDAAIMGSVVAAVSPAVIVPKMLQLMDEGYGTKESIPQLILAGASVDDV
                     FVIVMFTAFLGLAQGESINILSFVNIPLSILTGIICGALLGKGFAIFFKKNHMRDTVK
                     ILILLSIAFVLVTLEDILPIPFSGLIAIMFMGIFLQRNHSVASKRLSVKFNKLWVGAE
                     VLLFVLVGACVNISYALKAGMMAILLIFGVLLFRMIGVFICLFKTSLSKTERLFCMIA
                     YCPKATVQAAIGSVPLACGLGCGQIVLTVAVLSILITAPLGAFMIEHTYKKLLKKNKD
                     LKWPLCQGQ"
BASE COUNT          592 a          220 c          369 g          625 t
ORIGIN      
        1 ctaagaacaa agagttattg aatatctacc aggaaagtga aaagggtgat gtaaaagtgt
       61 gtagagcatt agatgaaatg tttgaagaat cgacaaacaa aggaatccag atgggtatca
      121 agcaaggcat tgaacaaggt attgagcaag gaattgaaca aggcattgaa caaggcattg
      181 agcgtggtgt aaaaaataca caaatcaaaa tagcgattaa tatgcttgta agaaataatc
      241 aaacgcttga agagatctct gagattgttg gattagacct aaatgcatta agagaactaa
      301 aaaagagtat ttgattgaat ggatgagcaa tcacatttat cttttcagta agtaaagaat
      361 tgattaaaaa agttattgat gaaggcttag atgtcgtagt ataatttttt tggggaatga
      421 agttctccct tagtttaata ctaaaaccgc tttttatagg ctgatgactt ctgtgttttt
      481 ttaacgcgga aaccatcagc ttttctgcgt tttgaaagga gagataaaat gctgatcagt
      541 ttatcttata tttttcttgt aggaatgttt ttatcttgga tctgtaaaaa ggtgggatta
      601 cctggactat taggaatgat atttacgggg attcttttag gaccttattg ccttggctta
      661 attgattcat ctttattaaa tatttctagc gaattaagaa agattgcatt aattattata
      721 ttgatgcgag ctggattgaa tctagattta agtgatttaa aaagagttgg aagacctgct
      781 atattgatgt gttttgtacc agcctgtttt gagatgattg gaatgatttt gttggcacca
      841 aaattactag gaatttcttt gttagatgct gccattatgg gaagtgttgt tgcagctgta
      901 agtcctgccg ttattgttcc aaaaatgtta caacttatgg atgaaggata tgggacaaaa
      961 gaaagtattc ctcaattgat tttggcagga gctagtgtag atgatgtttt tgtgattgtg
     1021 atgttcacag catttcttgg tttagcccaa ggagaaagta taaatatatt aagttttgtg
     1081 aatattccac tatccattct aactggcatt atatgtggtg cattattagg taaaggattc
     1141 gcaattttct ttaagaaaaa tcatatgcga gatacagtca aaattctaat tttattaagt
     1201 attgcatttg tccttgtaac acttgaagat atattgccaa ttccattttc aggattaatc
     1261 gcgattatgt ttatgggaat ttttctgcaa agaaatcatt cggttgcatc caaacgacta
     1321 tccgttaaat ttaataagtt atgggtaggt gcggaagttt tattatttgt gttagttgga
     1381 gcctgtgtaa atatttctta tgcactaaaa gcaggcatga tggctattct gttaatattt
     1441 ggagtacttt tatttagaat gattggtgta tttatttgtt tatttaaaac atcattaagt
     1501 aaaacggaaa gattattttg tatgatcgca tattgtccaa aggcaactgt gcaagcagca
     1561 attggatccg ttcctttagc ttgtggactt ggatgtgggc aaattgtatt gactgttgcc
     1621 gttttatcca tcttgattac tgcaccatta ggtgcattta tgattgaaca tacatataaa
     1681 aaattattaa aaaagaacaa agatttaaag tggcccctgt gtcaaggaca ataaataaaa
     1741 aaggtcttaa aattaaataa aaataaatat atcctctagg tgtcaaatac cggaggatat
     1801 atttta
//
LOCUS       DBMDWL010000282         1799 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_31419_length_1799_cov_1.693757, whole genome shotgun sequence.
ACCESSION   DBMDWL010000282 DBMDWL010000000
VERSION     DBMDWL010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31419_length_1799_cov_1.693757"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="ACZPDN_11985"
     CDS             <1..1697
                     /locus_tag="ACZPDN_11985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006526436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR1299374.1"
                     /translation="MKFTDYKYEHMDIEALQEQLKAICSKLQNAASYAEFKNAFIELN
                     DVNKYINTNQTLVDIRHTVDTRDEYYTQENDFLNEMLPVLKEDIVNCNKAVMASTFVS
                     ELRKEVPETYFLQREMEEKSFDPIIIPEMQEENKLASKYQAIIASAQIEFDGETYTLA
                     ALEVKTNDKDRDTRKRAMQAYWGWYDEHAKEIGEVYDQLVQVRTRMAKKLGYENYIEL
                     GYYRMMRFDYNKEDVENYRKQVLEDVVPLDNELYARQQKRLGYDTLHAWDEKFEFTSG
                     NPAPKYSREELVKRALKMYQELDPKTGEFFKFMTERELLDLDSKPGKAAGGYCTFIPN
                     YQSPFIFANFNQTSHDAEVLTHEAGHAFQVYSSKDIFPIDCVWPTYESCEIHSMSMEF
                     FTYPWMKSFFEEDVDKYYFNHLSGAVKFLPYGVLVDHFQHEVYENPTMSPEERLATWR
                     KLEKQYLPQKNYDGIEILEKGGWWMRQLHIFMDPFYYIDYTLAQVCALQFWARIQKKD
                     EKAFEDYKHICKIGGTLPFRKIVKEAGLKVPFESGCLKDTVQLVREWFENADDSQF"
BASE COUNT          659 a          241 c          337 g          562 t
ORIGIN      
        1 atatgaaatt tacagattat aaatatgaac atatggatat tgaagctttg caggaacaat
       61 taaaggcaat ttgttcaaaa ttacagaatg cagcttctta tgcagaattc aaaaatgcct
      121 ttatagaatt aaatgatgtt aataaatata ttaatacaaa tcagactctt gttgatatac
      181 gtcatacagt ggatacaaga gatgaatatt acacacaaga aaatgatttt ttaaatgaaa
      241 tgcttcccgt gttgaaggaa gatattgtca attgtaataa agcagttatg gctagtactt
      301 ttgtttctga attaagaaaa gaagtacctg aaacatattt cttacaaaga gaaatggaag
      361 aaaaatcatt tgatcccatt attattcctg aaatgcaaga agaaaataaa ttagcttcta
      421 aatatcaagc aatcattgca agcgcacaaa ttgagtttga tggtgaaaca tatactcttg
      481 ctgctttaga agtaaagaca aatgataaag atagagatac tcgtaaacgt gcaatgcaag
      541 cctattgggg atggtatgat gaacatgcta aagaaattgg tgaagtttat gatcaattag
      601 tgcaagttcg tacacgtatg gctaagaaat taggatatga aaactatatc gaacttggtt
      661 actatcgcat gatgcgtttt gattataata aggaagatgt tgaaaactat cgtaaacaag
      721 tattagaaga tgttgttcct ttggataatg agttatatgc tcgtcaacaa aaacgtttag
      781 gttacgatac tttgcatgct tgggatgaga agtttgaatt tacttcaggg aatccagcac
      841 ctaagtatag tcgtgaagaa ttagtgaaac gtgcattaaa gatgtatcag gaattagatc
      901 ctaaaacagg agaattcttt aaatttatga cagaaagaga attgttggat ctagactcaa
      961 aacctggtaa agcagcaggt ggatattgta cattcattcc taactatcaa tctccattta
     1021 ttttcgcaaa cttcaatcaa actagccatg atgctgaagt attaacgcat gaagcaggac
     1081 atgctttcca ggtatattca agtaaggata ttttcccaat tgattgtgta tggcctacat
     1141 atgagtcttg tgaaatccac tctatgtcaa tggaattctt tacatatcca tggatgaaat
     1201 ctttctttga agaagatgta gataaatatt atttcaacca cttatcagga gctgttaagt
     1261 tcttgcctta cggagtgtta gtggatcatt tccaacatga agtatatgaa aatccaacaa
     1321 tgtctcctga agaaagatta gctacttgga gaaagttaga aaaacaatat ttgcctcaaa
     1381 agaattatga tggaattgag attctagaaa aaggtggatg gtggatgcgt cagcttcaca
     1441 tcttcatgga tccattctat tatattgatt acacattagc tcaagtttgt gcattacaat
     1501 tctgggctcg tattcaaaag aaagatgaaa aagcttttga agactacaaa catatttgta
     1561 agattggtgg aacattgcca tttagaaaaa ttgtaaaaga agcaggatta aaagttcctt
     1621 ttgaatctgg atgtttaaaa gatactgtac aattggttcg tgaatggttt gaaaatgcag
     1681 atgattcaca attttaaaat agtagttacg catgaaaaat aaaatggctt aaataaacga
     1741 aatcggaaac tctattaaag aatttccgat tttttctagt gttcagatgg caatattaa
//
LOCUS       DBMDWL010000283         1782 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_31840_length_1782_cov_1.936347, whole genome shotgun sequence.
ACCESSION   DBMDWL010000283 DBMDWL010000000
VERSION     DBMDWL010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31840_length_1782_cov_1.936347"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="ACZPDN_11990"
     CDS             complement(<1..653)
                     /locus_tag="ACZPDN_11990"
                     /inference="COORDINATES: protein motif:HMM:NF025386.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299375.1"
                     /translation="MSTGPRGTYMNLSIPGTGIGYRTRLGGSNSSYRSTSSSSRYPYQ
                     KTIVNEYTGETRTVRASTQWELNEQVRNTEIRMKNNELRARRNAEIESQKEKAEQLTN
                     DVKQIQNSFKSIIATTIKVNDRLDWNKQLKNETYPAFSFAEPQPTKKKLGILNGLFGK
                     TDDYEDRLKEYEKRKTKALQDYFLAKQEFETEQRQHNADVNFLRESFEMQEEGAIEK"
     gene            complement(589..1158)
                     /locus_tag="ACZPDN_11995"
     CDS             complement(589..1158)
                     /locus_tag="ACZPDN_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014543.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HIR1299376.1"
                     /translation="MKKFKIFNWLKVVGVFFIFVIIVSVIGTFNTPKLDAITGEIATR
                     YIEMPSGKYAGDAILGNITGQGSFYFDTGEIYEGAWKDNEMSGKGKFTYTTGVYDGEF
                     SNSKRNGQGTFTWQDGSTYTGAWVSDKLSGEGTLTQTQAIYKGTFTDNIFKEGTITLT
                     KWWCKRCKDKYRTKRYIYESIHTRHRNWV"
     gene            complement(1145..1666)
                     /locus_tag="ACZPDN_12000"
     CDS             complement(1145..1666)
                     /locus_tag="ACZPDN_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299377.1"
                     /translation="MQLNHKGVDICERHAVIFLRKILQGYAENYMELRSPCGATVGQM
                     AYFYDGNVFTCDEGRMLYEMGNDAFKLGNVFENTFDDLILSPTCKAVCVSSLLEGQLN
                     CCDCVYQPYCGTCPVINLAQEKDLFFKNAKSYRCRVYGGMLDILFKILQENKAEDIEV
                     FNKWIGVEYNEEV"
BASE COUNT          520 a          379 c          231 g          652 t
ORIGIN      
        1 tacttttcaa tagcgccttc ttcttgcatt tcaaaacttt ctcttaagaa gtttacatcc
       61 gcattatgtt gtcgttgctc tgtttcaaac tcctgctttg ctaaaaaata atcctgcaaa
      121 gcctttgttt ttcttttctc atattcttta agtctatcct cataatcatc tgttttacca
      181 aacaatccat ttaatatacc caatttcttt tttgtgggtt gaggttcagc aaatgaaaat
      241 gcaggatatg tttcgttttt tagttgtttg ttccaatcca atctgtcatt tacttttatt
      301 gtagtagcaa taatgctttt aaaactattc tggatttgtt taacatcatt tgttaattgt
      361 tctgcttttt ctttttgact ctcaatttca gcatttcttc ttgctcttaa ttcgttattt
      421 ttcatacgaa tttctgtgtt acgaacttgc tcatttaatt cccattgggt acttgctctg
      481 acagttctcg tctcacctgt atattcatta acaatagttt tctgataggg atatcttgaa
      541 gaactacttg tacttctata actcgaatta gagcctccca atcttgttct atacccaatt
      601 cctgtgcctg gtatggatag attcatatat gtacctcttg gtcctgtact tatccttgca
      661 ccttttacac caccacttag tgagtgtgat tgtaccttct ttgaatatgt tatcagtaaa
      721 agttccttta taaatagcct gtgtttgtgt taaagtacct tcaccactta atttatcaga
      781 aacccaagcc cctgtatatg tagaaccatc ctgccaagta aaagtgcctt gaccatttct
      841 ctttgagttt gaaaactcac cgtcataaac acctgtggta taagtaaact tgcccttacc
      901 actcatttca ttatccttcc aagcaccttc ataaatttca cctgtatcaa agtagaaaga
      961 accttgtcct gtaatattac ctaaaatggc atcaccagca tattttcctg atggcatttc
     1021 aatatacctt gtagcaatct cacctgttat agcatctaat tttggtgtat taaaggtacc
     1081 tatcacagat actataatca caaatataaa gaatacacct acaaccttta accaattaaa
     1141 aatcttaaac ttcttcatta tattcaactc ctatccattt gttaaatact tcaatatcct
     1201 ctgccttatt ttcttgtaaa atcttaaata atatatctaa cataccacca tatactctgc
     1261 atctataaga cttggcattc ttaaagaata ggtctttttc ttgtgccaag tttataacag
     1321 ggcaagtacc acaataaggt tgataaacac aatcacaaca atttaattgt ccttctaata
     1381 acgacgatac acaaactgct ttgcaagttg gtgatagaat taaatcatca aaagtgtttt
     1441 caaaaacatt ccctaactta aatgcatcat tacccatttc atataacatt ctgccctcat
     1501 cacaggtgaa aacgttccca tcatagaaat atgccatttg acctactgtt gcaccacaag
     1561 gagaacgaag ctccatatag ttttcagcat atccttgtaa aatctttctt aaaaagatta
     1621 ccgcatgcct ttcacatata tctacacctt tatgattcag ctgcattata taccttaaac
     1681 atttgcttat gtgtgataaa gaagcaatag aagtgcaaaa aattttgccg ttcctatccg
     1741 acacttggcc attgtgtcgg ataggtcggg cggttattcg gg
//
LOCUS       DBMDWL010000284         1760 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_32361_length_1760_cov_2.002411, whole genome shotgun sequence.
ACCESSION   DBMDWL010000284 DBMDWL010000000
VERSION     DBMDWL010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32361_length_1760_cov_2.002411"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(249..944)
                     /locus_tag="ACZPDN_12005"
     CDS             complement(249..944)
                     /locus_tag="ACZPDN_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1299378.1"
                     /translation="MKALSIKHLNKNKILNDINLDIEAGEIVALVGPNGSGKSTLMKC
                     IMGFSNIDNGEIWIFDSLNNKNALKDVACSIEYPPLYPDLNGLDHFKLISQLNNINEQ
                     QMDNYIEFSGLKERLKKKSKYYSVGMRQMLILSLVIMQKPRLLILDEPFNGLDLQTKK
                     RVIDELENLNKQGTTILWSSHEINQLREMSDRYVFINCGKIVGHMKNEKKYKAECHED
                     PLEQYYFEVCKNV"
     gene            complement(1058..1483)
                     /locus_tag="ACZPDN_12010"
     CDS             complement(1058..1483)
                     /locus_tag="ACZPDN_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299379.1"
                     /translation="MYLKNKDFEKVENVFCVMKKEIIEKFNIIYTGNTALSTAICNIN
                     LGNTTIQHFVQCNTKEFNDSDLTIFFINTLNNLIEDNEPIIYVSIVNIGLNKAISIKT
                     NLAHDFSTVERDNHRFIKKYNLKVDKEEKKITFLHLNPK"
BASE COUNT          530 a          308 c          215 g          707 t
ORIGIN      
        1 gaatatacca atactgcaat tctttttctt tttccttatc atattgttga ctcaaatcgt
       61 atttcaatat gttgatatga ttctgttcat caactttcat tatccgtaaa ttttctattc
      121 ttccattctt taactccaaa tattctttgt ttgttttgac acagccacaa aacagtaaca
      181 aaacacatag cgcgatatta cagagtactt tttttgacat aaaattttta atttcaaatt
      241 ctagaaaatt aaacattttt acatacctca aagtagtatt gttccaaagg atcctcgtga
      301 cactctgctt tatacttttt ttcatttttc atatgcccaa cgatttttcc acaattaata
      361 aagacatatc tatcactcat ctctcttaac tgattgattt catgggatga ccatagtatt
      421 gttgtccctt gtttgttcaa gttttccagt tcatctatga ctcttttctt ggtttgtaag
      481 tctaacccgt taaatggttc atctagaatt aacagtcttg gcttttgcat tataaccaaa
      541 gataaaatca acatctgtct cattcctaca gaataatact ttgatttttt cttaagtctt
      601 tctttcaagc cagaaaactc tatatagtta tccatctgct gttcattaat attatttaat
      661 tgagaaatca atttaaaatg atccaatcca tttaaatcag gatataacgg tggatattca
      721 atgctgcaag ctacatcttt taatgcattc ttattattaa gagaatcaaa aatccagatt
      781 tcgccattat ctatatttga aaatcccatt atacacttca ttaatgttga ttttccagaa
      841 ccatttggac ctaccaaagc cactatttct cctgcttcta tgtctagatt tatatcgttc
      901 aagatcttat tcttatttaa gtgtttaata ctaagtgctt tcataataat acatgtttga
      961 aacagaacat attcaaactt tacctcttcc ttcttatttt tgttttcact ttaacctaga
     1021 aaataaagta aaacaaccac acggtatcat gtggttgtta tttgggatta agatgcaaga
     1081 aagtaatctt tttttcttct ttgtctactt ttaaattgta tttttttatg aatctatggt
     1141 tatctctttc aacagttgaa aagtcatgtg ctaggtttgt tttaattgaa attgctttat
     1201 ttaaacctat gtttactata gatacatata tgataggctc attatcctct atcagattat
     1261 tcaatgtgtt tatgaaaaat atagtcagat ccgaatcatt aaactctttt gtattacatt
     1321 gaacgaaatg ctgaatagtc gtattgccaa gattgatatt gcatattgcc gtgctcaaag
     1381 ctgtatttcc agtatagatg atattgaact tttcaatgat ttctttcttc atcacacaaa
     1441 aaacattctc tactttttca aaatctttat tttttaaata catatctata gaatgactat
     1501 catgtatcaa tgcatgtaaa aaatcaaagg tgttcacatt aaattatata tgtctttttg
     1561 actatctttt tatatcttcc taaatgtccg caaagcctta ttttatcggc tctacgggca
     1621 ttttgctttt gtggtaaacc ttgcatatct aggtctattt tcttatattt tccctgtcaa
     1681 tcgtggttaa aatcgtggta aatacttcta tgcgattaga cgctgaacct ctgattttgc
     1741 ggtatctatg gacgcatgag
//
LOCUS       DBMDWL010000285         1736 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_32974_length_1736_cov_3.514985, whole genome shotgun sequence.
ACCESSION   DBMDWL010000285 DBMDWL010000000
VERSION     DBMDWL010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32974_length_1736_cov_3.514985"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="ACZPDN_12015"
     CDS             complement(<1..397)
                     /locus_tag="ACZPDN_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299380.1"
                     /translation="MFRNKELFLTGRGKSIMDVEMEKRKFLNLCGKRDRALLSGEKRM
                     TLGDMERLTYLTEFFELENYGIALWKEFGDDVKEPFEAMMKMLDEPECIEDRWLDDEA
                     KGEKWLLEFQELALTEEYREWIRNYIDKKY"
     gene            479..715
                     /locus_tag="ACZPDN_12020"
     CDS             479..715
                     /locus_tag="ACZPDN_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1299381.1"
                     /translation="MQKIRPDMDIGKNIQAIRYKNNMTQDQVVAKLNLMGISITKSTY
                     AKLETNRMNIKASELVALAKLFHTDINAFFSGLL"
     gene            complement(1023..1295)
                     /locus_tag="ACZPDN_12025"
     CDS             complement(1023..1295)
                     /locus_tag="ACZPDN_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024094.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1299382.1"
                     /translation="MSKKLVAFFSASGTTKKVAQMIAEEAKADLFEIEPKVPYTKADL
                     DWMNKKSRSSVEMSDKKYRPEIMKKKMDMSSYDEILLGFPKMEYSL"
     gene            complement(1422..>1736)
                     /locus_tag="ACZPDN_12030"
     CDS             complement(1422..>1736)
                     /locus_tag="ACZPDN_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR1299383.1"
                     /translation="KPLILPERMNVQSELANWFGKDFSKLQIAFTSNLGTNAGIMAAN
                     GLGYPISIEGAATKYWREDILVQRRISPEITTSTVIAWRRNIPYSLAVRKMIEEINAF
                     QA"
BASE COUNT          508 a          382 c          213 g          633 t
ORIGIN      
        1 catatttttt atcgatataa tttcgaatcc attccctgta ttcttccgtt aatgccagtt
       61 cctgaaattc taacaaccac ttttcccctt ttgcttcgtc atcaagccat cgatcttcta
      121 tgcactccgg ctcatctaac attttcatca tcgcttcaaa tggctctttt acatcatcac
      181 caaattcttt ccataaggcg attccatagt tctccagttc aaaaaattct gtcagatatg
      241 tcaatcgttc catatccccc aatgtcattc ttttttcacc tgacaataat gctcgatctc
      301 ttttgccaca tagatttaaa aattttctct tttccatctc cacatccatt atacttttac
      361 ctcttcctgt taaaaataat tccttattac gaaacattat aaaatttaaa ttctttagaa
      421 tcaatatata tatttctgta ttacgaatct ttttatttta taaggggagt tcatcattat
      481 gcaaaagatt agaccagata tggatattgg taaaaatatc caagcaattc gatataaaaa
      541 caatatgact caagaccaag tagttgcaaa attaaatctt atgggtatct ccatcaccaa
      601 aagtacctat gccaaactgg aaacaaatcg catgaacatt aaggcatctg aattagttgc
      661 acttgcaaaa ctctttcata ccgatatcaa tgctttcttt tccggcttgc tatagttcga
      721 cattccccga ctccctgtgt atataatagc atattaaaaa taagaaccag tctatattct
      781 tatcggatat aaaccagttc ttttttctta tcaaataata taaaagtgac agggaattca
      841 gattcttatc ttctgtcact catgtaatta ttattctatt ttaaatttaa tgaatttacc
      901 cattcttgaa tttctttttc aatgttattt tctatataaa cgtcatacac atttttccac
      961 attatctgtg tatactgtta ttttcaaccc cataattata acttggtaca cgctatacac
     1021 tactataaac tgtactccat tttagggaat cccagaagga tctcgtcata agaactcata
     1081 tccatctttt tcttcataat ctccggtctg tatttcttat cactcatttc cacactgctg
     1141 cgggattttt tattcatcca gtcaagatca gcctttgtat atggaacttt tggctcaatc
     1201 tcaaataaat ccgcttttgc ttcctctgcg atcatctgtg ctactttttt tgttgttccg
     1261 cttgcactga aaaatgccac taatttttta ctcataataa aaagcccctt tctatacgta
     1321 tttttcacaa aaaaatagat gtataagatt tacattcatc ttacacatct attttaagct
     1381 ctatcgcttc attatgtcta atgcttatat tgaattttgt tttatgcctg aaaagcatta
     1441 atctcctcga tcattttacg gactgccaaa gaatatggga tattccgtcg ccaggcaatc
     1501 accgtactgg tcgtgatttc aggagaaatt cttcgctgta caagaatatc ctctcgccaa
     1561 tactttgttg cagcaccctc aattgaaatt ggatacccca gtccatttgc cgccataatt
     1621 ccggcattcg ttccgagatt actcgtaaaa gcaatctgta attttgaaaa gtctttccca
     1681 aaccagttgg caagttcact ttgaacgttc attctttccg gcaggatcaa gggctt
//
LOCUS       DBMDWL010000286         1697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_34007_length_1697_cov_2.713659, whole genome shotgun sequence.
ACCESSION   DBMDWL010000286 DBMDWL010000000
VERSION     DBMDWL010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34007_length_1697_cov_2.713659"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(164..1375)
                     /locus_tag="ACZPDN_12035"
     CDS             complement(164..1375)
                     /locus_tag="ACZPDN_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR1299384.1"
                     /translation="MNAVGIDVSKGKSMVAIMRPFGEIVSKPFEIKHTTSDIQSLVKL
                     INSIDGDSRIVMEHTGRYYEALAYQLATAGLFVSAINPKLIKDFDNDSLRKVKSDKAD
                     SVKIARYALDKWGELKQYSIVEEIRNQLKTMNRQFGFYMKHKTAMKNNLIGILDQTYP
                     GVNTYFDSPAREDGSQKWVDFATTYWHVDCVRKMSLNAFTEHYQNWCKRKKYNFSKSK
                     AEEIYGKAKELVPVLPKDDLTKLIIKQAIDQLNSASSTVEELRTLMDHTAAKLPEYPI
                     VMAMKGVGPSLGPQLIAEIGDVSRFTHKGALTAFAGVDPGVNESGSYAQKSVPTSKRG
                     SADLRKTLFQVMDVLIKTKPTDDAVYLFLDKKRAQGKPYYVYMTAGANKFLRIYYGRV
                     KECLASLPESS"
BASE COUNT          551 a          311 c          325 g          510 t
ORIGIN      
        1 atattgaaat gtatactcta gagaggaatg caacaaaaaa gcctgcaaat aaaaagtcaa
       61 ggctaaatta aaagaacatt gaaaaattta tacaggttga aaaaatggca tcacaataag
      121 accaccacaa tagtgctggt caaaaaatag gtgataaatt taattagctt gattcaggga
      181 gtgaggcaag acattctttt acacgcccat agtagatacg aagaaatttg ttcgcaccag
      241 ctgtcatata gacataataa ggttttcctt gtgctcgttt tttatcaagg aataaatata
      301 cagcatcgtc tgtcggtttt gttttgataa gaacatccat tacctgaaag agagtttttc
      361 gcaaatcagc agagcccctt ttagaagttg gtacactttt ctgagcataa gatccagatt
      421 catttacacc aggatccaca cccgcaaatg cagtaagggc acctttatga gtgaatctag
      481 aaacatcacc tatctcggct attagttgag gacccaaaga aggaccgaca cctttcatag
      541 ccataacgat tggatattca ggtagttttg ctgctgtatg atccataagc gtgcgtaact
      601 cttcgacagt gcttgatgca gagttgagtt ggtctatcgc ttgtttaata atgagtttag
      661 tcaaatcatc cttaggaagt acaggaacaa gttccttagc ttttccatat atttcttcag
      721 ctttagattt actgaagttg tacttcttgc gtttgcacca gttttgataa tgttcagtga
      781 atgcattaag agacatttta cgaacacagt caacgtgcca gtaagtggtt gcaaaatcta
      841 cccatttctg gctgccatcc tcacgagcag gactatcaaa gtaggtgtta acaccaggat
      901 aagtttgatc taagattccg attaggttat ttttcatagc cgtcttatgc ttcatataaa
      961 aaccaaactg acggttcatt gttttaagtt gattacgtat ttcctccaca atactatact
     1021 gtttaagctc gccccatttg tcaagagcat aacgggcaat tttaacggaa tcagctttgt
     1081 cggatttgac ttttcgaaga gaatcattat cgaagtcttt gataagttta ggattgatgg
     1141 cactaacaaa aagaccagct gttgcaagtt gataagcaag agcttcgtaa taacggccag
     1201 tatgttccat cacaatacgt gaatcgccat caatagaatt aataagtttt acaagtgatt
     1261 gaatgtcact tgtggtgtgt ttgatttcaa aaggtttaga tactatctca ccaaaaggtc
     1321 gcatgatagc aaccatactt ttaccttttg aaacatcgat acctactgcg ttcataaatt
     1381 tgtcactcct taataattat tgcaatggat aaataccagt tttactcatt gcctattcaa
     1441 tctactgtgg tgtgacacga acgtaccaaa ggcagttcaa cctgcataaa acgaacgctg
     1501 cgaatgagga gctggttatc agtcttttat tacggacgcg aagtccagga aagggagccg
     1561 atataccgat tgctccatca ttatacagct aaagcaacaa gatggataat tccttactgg
     1621 ctgtaaggga tattaaccat aaatatattg tagtagaaaa ggagaattac agtgaaaaga
     1681 tttagaaaaa gtataat
//
LOCUS       DBMDWL010000287         1635 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_35761_length_1635_cov_1.110821, whole genome shotgun sequence.
ACCESSION   DBMDWL010000287 DBMDWL010000000
VERSION     DBMDWL010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35761_length_1635_cov_1.110821"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="ACZPDN_12040"
     CDS             <1..1089
                     /locus_tag="ACZPDN_12040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1299385.1"
                     /translation="TVQLGNISYIICAIVGAILALNGHFALTIGTLVAFLTLNKSFNQ
                     PIGQISQQINAVAMAQAGAGRIFDLLDQESEDDQGVVTLCRVETVDGKMVETDKRTGH
                     WAWKHPRSDGHVELVEMKGDIRLEGVDFGYFDDRMVLHNVDVYANPGEKISFVGATGA
                     GKTTITNLINRFYDIQKGSITYDGIDIKLIKKDDLRRSLGVVLQDTHLFTGTVMDNIR
                     YGRLDATDEECIEAAKLANADLFVKHLPDGYQTMLTGDGANLSQGQRQLLAIARAAVA
                     NPPALILDEATSSIDSRTEKLVQDGMDKLMHGRTTLVIAHRLSTIKNSDCIMVLEQGH
                     IIERGNHQSLIKEKGKYYQLYTGAIEMD"
     gene            1171..1494
                     /locus_tag="ACZPDN_12045"
     CDS             1171..1494
                     /locus_tag="ACZPDN_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016629.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HIR1299386.1"
                     /translation="MIGNVDDPTEIKRYRDVVRKAGSHGDYVIIGVEHQSTFDKNMIF
                     RILNYDATTYINQVESKKEVYPVGNFVFYTGDEGCFADLCNYAFFKEDMSFNLKNLFQ
                     EKMIC"
BASE COUNT          567 a          225 c          339 g          504 t
ORIGIN      
        1 acagtacaac ttggaaatat ctcttatatc atttgtgcaa ttgttggcgc aatccttgct
       61 ttaaatggac attttgcttt aacaattgga acccttgtag ctttcttaac attgaataaa
      121 agtttcaacc aacctattgg acaaatttct caacaaatca atgctgttgc catggcacaa
      181 gcaggagctg gtcgtatctt tgatttatta gatcaagaaa gtgaagatga tcaaggtgtg
      241 gttactttat gtcgtgtaga aactgttgat ggaaaaatgg ttgaaacaga taaaagaaca
      301 ggacattggg cttggaaaca tccaagaagc gatggtcatg ttgaacttgt agaaatgaag
      361 ggtgatattc gtttagaagg tgttgacttt ggttattttg atgaccgtat ggttttacac
      421 aatgtagatg tttatgctaa tcctggtgaa aagatttcct ttgttggggc tactggtgca
      481 ggtaaaacaa caattacaaa cttaattaat cgtttctatg atattcaaaa aggttcgatt
      541 acttatgatg gaattgatat taaattgatt aaaaaagatg atttaagaag atcattaggt
      601 gtggttttac aagacacaca tttatttact ggaacagtta tggataatat tcgctatgga
      661 cgtcttgatg ctacggatga agaatgtatt gaagcagcaa aacttgccaa tgccgatctc
      721 tttgttaaac atttgccaga tggttatcaa acaatgctta caggagatgg ggcgaactta
      781 tcgcaaggac aaagacagtt attagcgatt gcgcgtgctg ctgttgctaa tcctcctgca
      841 cttattttgg atgaagcaac ttcttctatt gattctcgaa cagaaaagct tgtacaagat
      901 ggaatggata aattaatgca tggtagaaca acacttgtta ttgcccatcg tttatcaaca
      961 attaaaaata gtgattgtat tatggtctta gaacaaggac atattattga aagaggaaat
     1021 catcaatcac ttattaaaga aaaaggcaaa tactatcaat tatatacagg agctattgaa
     1081 atggattaat ccatcaatag ctttttatat tttttggact atccgtccat tgaaagaagg
     1141 aaaagagaaa gataaaatga tctgttgacc ttgataggaa atgtagatga tccaacagag
     1201 atcaaaagat atcgggatgt cgtaagaaaa gcaggtagcc atggagatta tgtgattatc
     1261 ggagttgaac accaaagcac ttttgataaa aatatgattt ttcgcatact caactatgat
     1321 gcgacgactt atatcaatca agttgaaagt aaaaaagaag tctatccagt aggaaacttt
     1381 gtgttctata cgggagatga aggatgtttt gcagatcttt gtaattatgc ttttttcaag
     1441 gaagacatgt cattcaacct gaagaactta tttcaagaga aaatgatctg ttgaccttaa
     1501 aataatggat gcaagaaagc ttatgaatcg aagattaaga gatgtcgtag aaattagtca
     1561 aagtatgttt gcaggaagtt atgatggatt aagagaaaat cgtaaaattg aaacagaaaa
     1621 cttcatgatg gcggc
//
LOCUS       DBMDWL010000288         1624 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_36096_length_1624_cov_2.004596, whole genome shotgun sequence.
ACCESSION   DBMDWL010000288 DBMDWL010000000
VERSION     DBMDWL010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36096_length_1624_cov_2.004596"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_12050"
     CDS             complement(<1..232)
                     /gene="tnpA"
                     /locus_tag="ACZPDN_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIR1299387.1"
                     /translation="MDKMDHNAHSVYLMCYHLIMVVKYRRNVIDDPISERAKEIWERI
                     SPDYGITLEEWNHDVDHVHVMFRAQPKSELSKF"
     gene            479..1600
                     /locus_tag="ACZPDN_12055"
     CDS             479..1600
                     /locus_tag="ACZPDN_12055"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR1299388.1"
                     /translation="MNENRPKGNQKHIDLSGRIRIEQGLNNGESFRTIARDLNMDPST
                     ISKEVRRHSVIKERKPDAFAPIPCANNYDASKPKANVCKILHGCGDNECTHKCVNCRK
                     ARCSEICNHYKPRQCEKLAKPPYACNGCPKKTNCMMDRKIYSSKYAQDTYETLRTTSR
                     EGINQTPESIQKLNDLLSPLIKKGQSIAHIYASHAEEIACSRRTIYSYIDSGVFEVRN
                     MDLRRKVVYKPRKKKTATSTKDRAFRKDRGYKEFQEYINKNKPTYIVEMDTVEGVKGT
                     KPCFLTMLFRNCKLMLMFLLKEQTQDEVNKVFDYLTEVLGIELFQKLFEVIITDNGHE
                     FQDRWNLECDDNGEMRTRIYYCDPNRSDQKGALEKITNT"
BASE COUNT          560 a          283 c          305 g          476 t
ORIGIN      
        1 taaatttgct gagttctgat tttggctgtg cccgaaacat aacatgcaca tgatcaacat
       61 catgattcca ttcttccagt gtgattccat aatcaggtga aatacgttcc cagatctctt
      121 ttgcacgttc cgaaatcgga tcatctatta cgtttcttcg gtattttaca accatgatca
      181 gatgataaca catcaaatat actgaatgtg cattatgatc cattttgtcc atataatcag
      241 tcctttctat tctttcgact gattatagta taccacactt tgtattgtag aacaagtgtt
      301 ctgctttctt tttctgcaat tcatctccca cctgtagagc ttgaatgaaa aagtaaattc
      361 tacctggcaa gagttaattc tacgtctgta gaattaactc ttgcatagat tttattcttc
      421 attttttgta caatgtcaag gcagttgatt gcctgcctgt gcagaaagtg aggttattat
      481 gaatgagaac cgaccaaaag gcaatcagaa gcatatagat ctatcaggaa gaattcgtat
      541 agaacaaggt ctaaataatg gcgaatcatt tagaactatt gctagagatt taaacatgga
      601 tcctagcact atatctaaag aggtaagacg tcattctgtt attaaagaac gtaagcctga
      661 tgcctttgca cctataccct gcgctaataa ttacgatgcc tctaaaccga aagccaatgt
      721 atgcaagata cttcatggat gcggagataa tgagtgcact cataaatgtg ttaactgcag
      781 aaaggcaaga tgcagtgaaa tttgtaacca ctataaaccg agacagtgtg aaaaacttgc
      841 taagcctcca tatgcatgta acggctgtcc aaagaaaacg aactgcatga tggatagaaa
      901 aatatattca tccaagtacg ctcaggatac ctatgagaca ctgagaacga caagcagaga
      961 aggtattaat caaacgcctg aaagcattca gaaattaaat gacctgctgt ctccgctcat
     1021 taaaaaggga caatcaatag ctcatatcta tgcttctcat gcagaggaaa ttgcttgttc
     1081 aagaagaact atttactcct atattgatag tggtgttttc gaagtcagaa atatggattt
     1141 acgtcgtaag gtcgtataca aaccaaggaa gaaaaaaact gccaccagta caaaagatcg
     1201 tgcatttaga aaagatagag gctacaaaga gtttcaagaa tatataaata aaaataaacc
     1261 tacatacatc gtagaaatgg atacagtgga gggtgttaaa ggcacaaaac catgtttttt
     1321 gacgatgctt tttagaaact gtaagttaat gcttatgttc cttttgaaag agcaaactca
     1381 ggatgaggtc aataaagttt ttgactattt aactgaagtc cttggcattg aattatttca
     1441 aaaactattt gaggtaataa tcacagataa tggacatgaa ttccaggata gatggaattt
     1501 agagtgtgat gataacggtg aaatgagaac gcgaatttat tattgtgatc ctaatcgttc
     1561 tgaccaaaag ggtgcgcttg aaaaaatcac gaatacataa gatatgttct tcctaaagga
     1621 acaa
//
LOCUS       DBMDWL010000289         1620 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_36209_length_1620_cov_3.105991, whole genome shotgun sequence.
ACCESSION   DBMDWL010000289 DBMDWL010000000
VERSION     DBMDWL010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36209_length_1620_cov_3.105991"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="ACZPDN_12060"
     CDS             <1..301
                     /locus_tag="ACZPDN_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR1299389.1"
                     /translation="LIKAGVKIYEYTPGFIHGKVCIIDDSSALVGTVNMDFRSYYQHY
                     ECGVWMHEVDCLKDIKNDFEEIFKVSHEVSLEECINTNPIVKVYRNVLKVFSPIM"
     gene            complement(322..>1620)
                     /locus_tag="ACZPDN_12065"
     CDS             complement(322..>1620)
                     /locus_tag="ACZPDN_12065"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR1299390.1"
                     /translation="TQRSLENFKIGQELMPKSLIHSFAILKEACAKANLHFNKISEKQ
                     CEAIVKVCQNIEDGQYLDQFPLHVWQTGSGTQTNMNANEVISMLGNEYAKENILHPND
                     TVNASQSSNDTFPAALHIMVAQKINEELLPRLNQMIAQIKKLEEENEGIIKIGRTHLQ
                     DATPLYFSQELSGYRSMIEHSKDQIVDSLKYVYELACGATAVGTGINCPEGFDEIVTK
                     IISEKTNLPFIPDSNKFHALTSKDAMVYTSGALKGLAANCMKIANDVRWLASGPRSGI
                     HEIDIPSNEPGSSIMPGKVNPTQCEALTMVAVQVMGNDAAIGIGASQGNFELNVFMPL
                     IAYNMDQSIQLLSDAIHSFTIHCLDGLKANKKTMDKNLHNSLMLVTCLNPVIGYEKAG
                     HASQYAFKNNLTLKEAILELGYLTSEEFDLYVQPEKMIHK"
BASE COUNT          479 a          261 c          280 g          600 t
ORIGIN      
        1 attaatcaag gctggtgtta aaatctatga atatacacct ggttttatcc atggcaaagt
       61 ttgtatcatt gatgattcta gtgctttagt aggtacagtg aatatggatt ttagatcgta
      121 ttatcaacat tatgaatgtg gtgtatggat gcatgaagta gattgtttga aagatataaa
      181 aaatgacttt gaagaaatat tcaaagtctc acatgaagtt tcattagaag agtgtataaa
      241 tacgaatcca attgtgaaag tatatcgaaa tgtattaaag gtatttagtc ctattatgta
      301 ggaataaata ccttttgtgt tttatttatg aatcattttt tctggttgaa catacaaatc
      361 aaattcttca cttgtcaaat aacctaattc taaaatggct tcttttaaag ttaaattatt
      421 tttgaaggca tattgacttg catgacctgc tttttcatag ccgatgactg gatttaaaca
      481 agttactaac attaatgaat tatgtaagtt tttatccata gtttttttgt ttgcctttaa
      541 tccatctaaa caatgaattg taaaagagtg gattgcgtca cttaataatt gaatgctttg
      601 atccatatta taggcaatta atggcataaa tacattcagt tcgaagtttc cttgacttgc
      661 accaataccg atagctgcat catttcccat gacttgaaca gcaaccattg tcaaggcttc
      721 acattgcgta ggattgactt ttcctggcat gatacttgat cctggctcat tgcttggaat
      781 atcaatttca tgaattccac ttcttggccc actggcaagc catcttacat cattggcaat
      841 cttcatgcag ttagccgcaa gtccttttag agcaccactt gtatagacca tcgcatcctt
      901 acttgtaagg gcatggaatt tatttgaatc aggaataaat ggaaggttgg ttttttcaga
      961 aatgattttt gtgacgattt catcaaaacc ttctggacaa ttgattccag tacctacagc
     1021 ggttgcacca catgcgagtt catatacata ttttaaactg tctacgattt gatctttact
     1081 atgttcaatc atagaacggt atccgcttaa ttcttgtgaa aaatataatg gtgtcgcatc
     1141 ttgaagatgg gttctaccaa ttttgatgat tccttcattt tcttcttcaa gctttttgat
     1201 ttgtgcaatc atttgattta atcgtggaag taactcttca ttgatttttt gtgcgaccat
     1261 gatatgtaaa gcagcaggga aagtatcatt tgaactttgg gatgcgttga ctgtgtcatt
     1321 tggatgaaga atattttctt ttgcgtattc atttcctaac atagaaatga cttcattggc
     1381 attcatattc gtttgtgtac cggaacctgt ttgccaaaca tgcaaaggaa attggtctaa
     1441 atattgtccg tcttctatgt tttgacatac tttaacaatg gcttcacatt gtttttcact
     1501 gattttattg aaatgaaggt tggctttggc acaagcttct ttcaaaatcg caaaactatg
     1561 aattaaagat tttggcatta attcttgtcc aatcttaaaa ttctctaaac ttctttgtgt
//
LOCUS       DBMDWL010000290         1611 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_36510_length_1611_cov_1.927152, whole genome shotgun sequence.
ACCESSION   DBMDWL010000290 DBMDWL010000000
VERSION     DBMDWL010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36510_length_1611_cov_1.927152"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="ACZPDN_12070"
     CDS             <1..474
                     /locus_tag="ACZPDN_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR1299391.1"
                     /translation="LNLFTNRIKDIRILKDAKEMVYLNVGRNDIKDITCLKDMKQLEI
                     ISLENNNVKDLKPLSELTNLCDVDLYNNVIEDLTPLENLEFISYLRLDHNAITDLTPL
                     SKLKYLRSLTLKANYITDVTPLKDLELLEALRLDDNPIQDTSVLESMEFYEKFTN"
     gene            complement(894..1265)
                     /locus_tag="ACZPDN_12075"
     CDS             complement(894..1265)
                     /locus_tag="ACZPDN_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299392.1"
                     /translation="MDENETFKHHEGQSVTFHDSLIEHYCMIIKPKKAASDGTIHYTT
                     NEIAGVINKALNMLQNITNFYNRKDKDSYAKILSITTISKHRIKKSINVLDPIGKIQL
                     SKETMDRIDTEIVKAITNIAL"
     gene            complement(1414..>1611)
                     /locus_tag="ACZPDN_12080"
     CDS             complement(1414..>1611)
                     /locus_tag="ACZPDN_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1299393.1"
                     /translation="LENKYKNIKKSSYANIYQITTIDKSKIVNPMNHLDPIKRIKAPD
                     SVMDKIDKGIIEAFTQAKEKQ"
BASE COUNT          529 a          240 c          252 g          590 t
ORIGIN      
        1 ttaaatttat tcacaaaccg tatcaaggat attcgtattt taaaggatgc caaagaaatg
       61 gtatatttga atgtgggccg caatgatatt aaggatatta cttgtttaaa agatatgaaa
      121 caattggaaa tcatttcttt agaaaataat aatgttaagg atttaaaacc attatccgaa
      181 cttacgaatc tttgcgatgt ggatttatat aataatgtca ttgaggattt aacaccgtta
      241 gaaaatctag agtttatttc ttatttacga ttggatcaca atgccattac ggatttaact
      301 ccattatcaa aacttaaata tttgcgttct ttaactttga aggcaaatta tattacagat
      361 gtaactccat taaaagattt agaattatta gaagcactac gtctagatga taatcctatt
      421 caagatacaa gtgtattaga aagtatggag ttctacgaaa aatttacgaa ttaaacaaaa
      481 tcccctttaa acaaagggga tttactactt ttatataaaa ttataactta tttatgactt
      541 aaaaaattga aattgcgttt aaaatcttat cttcctcagt gttaatcaag tgtcaatagg
      601 taaccgttgt ggtacgaatg agataccaca cctgatacgt agttttgatg cgatagtcat
      661 ctgttcacgt gagagtattg gtagtactct gaattgtttt ggatgttggt agcatccagg
      721 attttttatt ttgcttcgtt aaaaagaaaa agctatccga aaccgaatag ctttcccata
      781 tctgcggcac ccacaaatgt gttaccgaaa taaataccag cagtgcctga tactatgtat
      841 catcactgaa attcatcttt ataataacat gaatttcttt aaaatcaagt gattcacaag
      901 gcaatgttgg ttatagcttt tacaatttca gtgtcaattc tgtccattgt ttctttagat
      961 aactgtattt taccaattgg atctaataca ttaatcgatt ttttaattct atgtttacta
     1021 attgttgtta tagacaaaat ctttgcatat gaatctttgt cttttctgtt gtagaaatta
     1081 gttatatttt ggagcatatt caaggctttg ttgataacac ctgcaatttc attagtcgta
     1141 taatgaattg taccatcaga agcagccttc ttcggtttga ttatcataca ataatgttca
     1201 atcaaagaat cgtgaaacgt gaccgattgc ccttcatgat gtttaaaagt ttcgttttca
     1261 tccattaata aaccttctat tattgcagaa aaagctactg agataaactc agtagctttc
     1321 aataaatcgg cgctaaggcc ataatgtgaa tctaatcagc gctaaggcca taattcaatt
     1381 atatcttaat tgaaaagtac tgaattgtca attctattgt ttctctttgg cttgtgtaaa
     1441 agcttctatg atacctttat cgattttgtc cataacacta tcaggagctt tgattctttt
     1501 gattggatcc agatgattca tggggttaac aatcttagat ttatctatcg ttgtaatttg
     1561 ataaatattg gcataagaag attttttgat gttcttatat ttattctcaa g
//
LOCUS       DBMDWL010000291         1594 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_37083_length_1594_cov_0.759545, whole genome shotgun sequence.
ACCESSION   DBMDWL010000291 DBMDWL010000000
VERSION     DBMDWL010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37083_length_1594_cov_0.759545"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="ACZPDN_12085"
     CDS             <1..367
                     /locus_tag="ACZPDN_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1299394.1"
                     /translation="HYQQLLIVFSTLIKDTKKYGYDLFSQMFIANLKENRHSFDMRDN
                     LTKLCIIIIKKAQEQKEIHNLSNPEILYQALAHAFTGHEALWCIKKDSPCFDEAFYLR
                     MNALLEVDSTYQDLYKEYL"
     gene            408..>720
                     /locus_tag="ACZPDN_12090"
     CDS             408..>720
                     /locus_tag="ACZPDN_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1299395.1"
                     /translation="MLIECSNIKKSFQGIDLLKDISFKVDDHDKIAIIGVNGAGKTTI
                     LNILIGEESYDSGDLFKSRDLNIGYLKQHHDLDMNKTIYECALEVFDPLIQIENRMRE
                     LE"
     assembly_gap    721..730
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <731..>1594
                     /locus_tag="ACZPDN_12095"
     CDS             <731..>1594
                     /locus_tag="ACZPDN_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1299396.1"
                     /translation="DGFSYPSRIVGVLKGMGFVEEDFQKYVHELSGGQKTRLCLAKLL
                     LEEPKLLVLDEPTNHLDSQAISFLENYLKGYKNALIVVSHDRYFIDVVSNKIIEIENG
                     KSHSYNCRYEEYSIQKRTQRAIDLKHYINQQKEIKKIQESIDTLKSFNREKSIKRAES
                     KEKQLAKIERIDRPENLPSAITVQFTPAIESGYDVLKVNDLSMRYDQIVFEHCNLDIK
                     KGERVAIVGENGVGKTTLFKALVGQLPIEHGKIRFGSHVEMAYYDQEHESLNYNKTIF
                     DEISDCYPRMT"
BASE COUNT          599 a          200 c          263 g          522 t
ORIGIN      
        1 tcactatcaa caattactta ttgtcttttc tacattaatt aaagatacta aaaaatatgg
       61 ttatgattta tttagtcaaa tgtttattgc gaatttaaaa gaaaatcgtc atagctttga
      121 tatgcgtgat aatcttacaa aactttgtat catcattata aaaaaagccc aagaacaaaa
      181 agaaattcat aatttatcga atccagagat tttatatcaa gctttagcgc atgcctttac
      241 aggacatgaa gctttatggt gtattaaaaa agattcacct tgttttgatg aagcttttta
      301 tctaagaatg aatgctttat tagaagtaga ttctacttat caagatttat acaaagaata
      361 tttgtaaaca acttatttta ttgatagaat agtcgaggtg atgaaaaatg cttatagaat
      421 gttcaaatat aaagaaatca tttcaaggaa ttgacttatt aaaagatatt agttttaaag
      481 tagatgacca tgataaaatt gcgattatcg gtgtcaatgg ggcgggtaaa acaaccatcc
      541 taaatatctt aattggtgaa gaaagttatg atagtggtga tttatttaaa agccgtgatt
      601 taaatatcgg ctatttaaaa cagcatcatg atttagatat gaataaaacc atttatgaat
      661 gtgctttaga agtctttgat ccacttattc aaatagaaaa tcgtatgcgt gaattagaaa
      721 nnnnnnnnnn agatggtttt agttatccaa gtcgtattgt cggggtttta aaaggaatgg
      781 gttttgtgga agaagacttt caaaaatatg tccatgaatt atctggaggt caaaaaacca
      841 gactttgtct tgccaagctt ttattagaag aacctaaact tttggtttta gatgaaccaa
      901 ccaaccattt agatagtcaa gcaatttcct ttttagaaaa ctatttaaaa ggatataaaa
      961 atgctttgat tgtggttagc catgaccgtt attttattga tgttgtttct aataagatca
     1021 ttgaaataga aaatggaaaa agtcatagtt ataattgtcg ttatgaagaa tattctattc
     1081 aaaaaagaac acaacgtgcg attgatttaa aacactatat caatcaacaa aaagaaataa
     1141 aaaaaataca agaaagtatt gatacattaa aatcattcaa tcgtgaaaaa agtattaaac
     1201 gtgcagaatc gaaagaaaaa caacttgcaa agattgaacg tattgaccga ccagaaaatt
     1261 tgccaagtgc catcactgtt caatttacac cagctattga atcaggttat gatgttttaa
     1321 aagttaatga tttatcgatg cgttatgatc aaattgtttt tgaacactgt aatcttgata
     1381 tcaaaaaagg agaacgcgtt gcgattgttg gtgaaaatgg tgttggtaaa accactttat
     1441 ttaaagctct tgtaggtcaa ttaccaattg aacacggtaa aattcgtttt ggtagtcacg
     1501 tagaaatggc ctactacgat caagaacatg aatctttaaa ttataataaa acgatttttg
     1561 atgaaatcag tgattgttat ccacgtatga ccaa
//
LOCUS       DBMDWL010000292         1582 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_37450_length_1582_cov_2.073599, whole genome shotgun sequence.
ACCESSION   DBMDWL010000292 DBMDWL010000000
VERSION     DBMDWL010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37450_length_1582_cov_2.073599"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(135..791)
                     /locus_tag="ACZPDN_12100"
     CDS             complement(135..791)
                     /locus_tag="ACZPDN_12100"
                     /inference="COORDINATES: protein motif:HMM:NF039504.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HIR1299397.1"
                     /translation="MCASLAPKYVKRNYTEILKHINLDCVYLDVFSCNEMDECFNLEH
                     LMTRKECMEYRRACFRYMISKGIIPSSEECSDWAMRELVFSHYGPYEFMMKDENEKRM
                     GIPVPLFNLVYHDCFILPWPMEKRQEDYMLYALLNGGIPYVIRNAPYDNVDGNFGSDG
                     LSIEDRIKRANVVLDFYQKIKNEEMVEHKIINDHVQQTTFSNNITIEINTKENTYRIV
                     "
     gene            complement(798..>1582)
                     /locus_tag="ACZPDN_12105"
     CDS             complement(798..>1582)
                     /locus_tag="ACZPDN_12105"
                     /inference="COORDINATES: protein motif:HMM:NF039504.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HIR1299398.1"
                     /translation="YMQGLLIPNHYDYDYSKIAFDGQFTSCAAYMPWLSQTNNGLGYI
                     AINETPWDSKYTIDHDDKGTRLQFVWLTSLGKMRYKRVVRYSFESNMDYNRAAKIYRE
                     YVKETGLFKSLKEKEVTLSKISELQQCAVVHTGIKAHTEKDSRFYNGQEDVIHSFDSV
                     KEMIQSLHKLGSHKLYLHLDGWADPGYDNCHPDYLPACIEAGGWNALEILQKSLSSQN
                     DLFGLHDQYRDYYYTAKTHNENEAIQLEDGSVFEHANWAGGR"
BASE COUNT          506 a          325 c          205 g          546 t
ORIGIN      
        1 aatttgatca tttgcacttt caatcaattc ctcatctgaa taagggattg aagtttgaac
       61 ttcttttgtc tctctaagtt cgggttgtgc ctttgtacaa gctgttaata atagtaaaac
      121 caaaactaat attttcatac aattctatac gtgttctcct ttgtgttgat ttcaattgtg
      181 atgttattac taaaagtggt ctgttgaaca tgatcattga tgattttatg ttctaccatc
      241 tcttcatttt taattttttg ataaaaatca agaacaacat ttgcacgttt aattcgatct
      301 tctatggata aaccatcact tccaaaatta ccatccacat tatcatacgg tgcatttctt
      361 atgacatatg gaataccacc atttagtaat gcatacaaca tgtagtcttc ttgccttttt
      421 tccattggcc aaggcaaaat gaaacaatca tgatatacaa gattaaataa tggtacaggt
      481 atacccattc ttttctcatt ttcatctttc atcataaact cataaggccc ataatgcgaa
      541 aatacaagtt cgcgcattgc ccaatcacta cactcctcac ttgaaggaat gattcctttt
      601 gagatcatat atcgaaaaca agcccttcta tattccatac actcttttct tgtcatcaaa
      661 tgttctaaat taaaacactc atccatttca ttacaagaaa aaacatctaa gtatacgcaa
      721 tctaaattaa tatgttttaa gatttcagtg tagtttctct ttacatactt tggtgcaaga
      781 cttgcacaca agtagtttta tcttcctcca gcccaattgg catgttcaaa cacactacca
      841 tcctctaatt gaatcgcttc attttcatta tgtgttttag ccgtataata ataatcacga
      901 tactgatcgt gtaatccaaa tagatcgttt tgtgaactca aacttttctg aaggatctct
      961 agtgcattcc atccaccagc ttcaatacat gccggtaaat aatccggatg acaattatcg
     1021 tagccaggat ctgcccaacc atctaaatga agatagagtt tgtgtgagcc taatttatga
     1081 agactttgaa tcatctcttt taccgaatca aaagaatgaa ttacatcctc ttgtccatta
     1141 taaaaccgag aatccttttc cgtatgcgcc ttaatacctg tatgtacaac tgcacattgt
     1201 tgaagttctg atattttaga aagtgtaact tctttttcct ttaaagactt aaataaaccc
     1261 gtttctttaa catactctcg ataaatctta gccgcacgat tataatccat attactttca
     1321 aaagaatatc gaacaacacg cttatatcgc atcttaccta aacttgttaa ccatacaaat
     1381 tgaagccttg tccccttgtc atcatgatca attgtatatt tagaatccca aggtgtttca
     1441 ttgattgcga tataccctaa tccattattt gtctgactaa gccaaggcat ataagcagca
     1501 caactagtaa actgaccatc aaaggcaatc ttactataat cataatcata atgattggga
     1561 atcaataaac cttgcatata ag
//
LOCUS       DBMDWL010000293         1571 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_37792_length_1571_cov_3.023129, whole genome shotgun sequence.
ACCESSION   DBMDWL010000293 DBMDWL010000000
VERSION     DBMDWL010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37792_length_1571_cov_3.023129"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..1482
                     /locus_tag="ACZPDN_12110"
     CDS             55..1482
                     /locus_tag="ACZPDN_12110"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR1299399.1"
                     /translation="MAKLTNEQKIEIYNKRKMGYTLNMLSTEYNINVHGIEYLVRLID
                     KNGFDVLRKDRNRYYSPKFKEKALRRVFIDGESITAVAIDIGLSSRGILTNWIKSYVE
                     NGYNIIEKKRGQHDKQKEKNDTVRNAEKDQGTGTSEPSLTCRERILKKIGCLSYGTKE
                     ERTKEIVKAITELRQELNLSLAFILDTINSNEDLPHIAKSVYYYTLSKIDKDDKNDEI
                     MVEIINIFYTHKERYGYRRIHLELLNKGYKVSHGKVKRLMSRMGLYAFNPTPKQRYNS
                     YKGDMNGVCKNLLLERKKNKYTGKINEKRNFEATSVNQKWTTDVSEFKIAAGKLYLSP
                     IMDIYNREIVGFDISDTPNLFQIYRMLDMAFEKFDNLEGLIFHSDQGWQYQHFSYHKI
                     LEEKGIFQSMSRKGNCLDNSPMENFFGKMKNEMFYGFENTFESLDNLKQAMIDYIHYH
                     NNFRITVKGKGLTPLQIRNQALSLS"
BASE COUNT          622 a          203 c          270 g          476 t
ORIGIN      
        1 cgaatatgaa gtgaactgaa aaagttggac aacttaggag gttcactttt tattatggct
       61 aaacttacaa acgaacaaaa aattgaaatt tacaataaaa gaaaaatggg atacacatta
      121 aacatgcttt cgactgaata taacatcaat gtgcacggta ttgaatatct tgtacgattg
      181 atagataaaa atggattcga tgttcttaga aaagatagaa acagatatta ttctcccaaa
      241 tttaaagaaa aagcattgag aagagtgttt atcgatggag aatcaattac agcggtagct
      301 attgatattg gcttatcaag tagaggaata ttgaccaatt ggattaaatc ttatgttgaa
      361 aacgggtata atattattga aaagaaaaga ggacaacatg acaaacaaaa agaaaaaaat
      421 gacacagtca gaaatgcaga aaaagatcaa ggaactggaa cttcagaacc ttcgcttacg
      481 tgtcgagaac gaatacttaa aaaaattgga tgccttagtt atggaacgaa agaagaaaga
      541 actaaagaaa tagtaaaggc tataacagaa ctaaggcagg aattaaatct gagcttagcc
      601 tttattctgg atactattaa ttctaatgag gatcttccac atattgccaa atccgtttat
      661 tattacactc ttagtaaaat tgacaaagat gataagaacg atgaaataat ggtggaaata
      721 atcaatatct tttacaccca caaagaacgt tatgggtatc gaagaattca tctagaattg
      781 ttgaataagg gatataaagt gtctcatggc aaagtaaagc gtctaatgtc gcgtatgggc
      841 ctatatgcat ttaatcctac acctaaacaa agatacaact cttataaagg ggatatgaat
      901 ggtgtttgta agaatcttct tctagaaaga aagaaaaata aatacacagg aaaaattaac
      961 gaaaaaagaa attttgaagc tacttctgta aatcagaaat ggactacaga tgtatccgag
     1021 ttcaagatag cagcaggaaa attatattta tctccaatta tggatatata caatcgtgag
     1081 atagttggat ttgatatttc agacacacct aatctattcc aaatatatag aatgttggat
     1141 atggcttttg aaaaatttga caatctcgaa ggcttgattt ttcattctga tcagggatgg
     1201 caataccaac atttttcata tcataaaata ttagaagaaa aaggaatttt ccaatctatg
     1261 tctcgtaaag gaaactgttt agataattct ccaatggaaa atttctttgg gaaaatgaaa
     1321 aatgaaatgt tttatggatt tgaaaataca tttgaatcat tagataattt aaaacaagcg
     1381 atgatagatt atattcatta tcataataat tttagaatta cagtaaaggg aaaaggctta
     1441 actcctttac aaattaggaa tcaagcctta tcattaagtt aattattttt tgtccaacaa
     1501 agtcggttca cttcagtttt tcctgttttt tatatagaat tattttttct aattttgtaa
     1561 gagctttcat t
//
LOCUS       DBMDWL010000294         1547 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_38619_length_1547_cov_1.985477, whole genome shotgun sequence.
ACCESSION   DBMDWL010000294 DBMDWL010000000
VERSION     DBMDWL010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38619_length_1547_cov_1.985477"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            217..1329
                     /locus_tag="ACZPDN_12115"
     CDS             217..1329
                     /locus_tag="ACZPDN_12115"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR1299400.1"
                     /translation="MNTINISQKTKNKHLDFSHYEYIINSLIMFNASYSGKRNIGKTQ
                     FIKNLALDVGTSVSNIYSIIKDATVSIRDTHLNKHFELSALAAFQKRSKNHKIPNNSK
                     FDKATDFISLVENEIKSNKLSSIDETINYLIIHQRDKINDMETVSTKTFYNYVHQGKT
                     SIKPIDLPRMVRRKTKKNWKTYIPKRQKGVSITERPESVDSREAFGHWEGDLVTGPRD
                     GQNGAYLTLLERKTRFYYMIPISSKSSKKVYMQINKLHKFYGDSFKDIFKSITFDNGS
                     EFSRWKDMEVNPNTKEKRTKIYFGRPYHSCDRASNENCNGLIRYFIQKGTDINTIDKK
                     TSIDINNKINQKKRKILGYLPAEKLFLDELAKLNVT"
     gene            complement(1458..1534)
                     /locus_tag="ACZPDN_12120"
     tRNA            complement(1458..1534)
                     /locus_tag="ACZPDN_12120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1498..1500),aa:Arg,seq:tct)
BASE COUNT          546 a          259 c          240 g          502 t
ORIGIN      
        1 atcagaatat caatgtcaat aaaataatgc gacaaaatcg aatatttttt attccctgta
       61 ttgtaaaatg aaactggaat taaggaattg ataacaaggg gcatgcccct tgttatcaat
      121 tcgcgcctgt gacatgcccc tcatgtttat tttctatatg aaaattgtcc atagaaatcg
      181 tattatattg ttaccacact ttataaggag atctctatga acactattaa tatatcacaa
      241 aaaacaaaaa ataaacatct cgatttttct cattatgagt atatcatcaa ttccttgatt
      301 atgtttaacg cttcttattc tggtaagcgc aacatcggta aaactcaatt tattaaaaat
      361 cttgctttgg atgttggtac ttcggtttct aatatctatt caattatcaa agatgctacg
      421 gtctctatta gagatactca tctcaataag cattttgaac tatctgcctt ggctgctttt
      481 caaaaaagat ccaagaatca taagattcct aacaattcta aatttgataa agctactgat
      541 tttatttctc ttgttgaaaa cgaaatcaaa tccaacaagc tttcttctat tgatgaaacc
      601 atcaattatt tgattattca tcaaagggat aaaattaatg atatggaaac tgtttccact
      661 aaaacctttt ataactatgt tcatcagggt aaaacctcta ttaaacctat cgatcttcct
      721 cgcatggtca gaagaaaaac aaagaagaac tggaagacat atattcctaa aagacaaaaa
      781 ggggtttcta ttactgaaag gcctgaatct gtcgattcca gagaagcgtt tggtcattgg
      841 gagggcgatt tggttactgg tcctagagat ggtcaaaatg gtgcttatct tactttgttg
      901 gaaagaaaaa ctcgatttta ctatatgatc cctatttctt ccaaatcttc taaaaaagta
      961 tatatgcaaa tcaacaagct tcacaagttt tacggtgata gctttaaaga tattttcaaa
     1021 tccatcactt ttgataacgg tagtgaattt tctagatgga aggatatgga agtaaatcct
     1081 aatacaaaag aaaagagaac caagatttat tttggcagac cctatcattc ttgtgacagg
     1141 gcctcaaatg aaaactgtaa tggacttatc agatatttta tacagaaagg tactgacatc
     1201 aacacaatag ataaaaagac aagtattgat ataaataaca aaatcaatca aaagaaaagg
     1261 aagatacttg gatatcttcc tgctgaaaaa ttatttctag atgaattagc taaacttaat
     1321 gtaacttaaa atacgatttt ctataaaaat tagttcctat tttctcttat ttccagtttc
     1381 tgcttgcaat tcgggaaatt tatattttga agagatatat aaaataaaaa acaacccgaa
     1441 ggttgttgtt tattaaatgg cgtccacgaa gggattcgaa cccctgaccg cacgcttaga
     1501 aggcgtgtgc tctatccagc tgagctacgt agacattgct tactttc
//
LOCUS       DBMDWL010000295         1532 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_39131_length_1532_cov_2.728162, whole genome shotgun sequence.
ACCESSION   DBMDWL010000295 DBMDWL010000000
VERSION     DBMDWL010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39131_length_1532_cov_2.728162"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(83..1291)
                     /locus_tag="ACZPDN_12125"
     CDS             complement(83..1291)
                     /locus_tag="ACZPDN_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR1299401.1"
                     /translation="MNAVGIDVSKGKSMVAIMRPFGEIVSAPFEIKHTASGINSLVEL
                     INSVEGESRIVMEHTGRYYEVLAHQLSEANLFVSAINPKLIKDFDNDSLRKVKSDKAD
                     AVKIARYTLDKWQNLKQYNVMDELRNQLKTMNRQFGFYIKHKTAMKNNLIGILDQTYP
                     GVNTYFDSPARSDGSQKWVDFASTYWHVDCVRKMSLNSFIDHYQNWCKRKKYNFSQSK
                     AEEIYGKAKELVPVLSKDAITKLIIKQAVDQLNSASTTVESLRTLMNETASKLPEYPV
                     VMAMKGVGTSLGPQLIAEIGDVSRFTHKSAITAFAGVDPGVNESGSYEQKSVPTSKRG
                     SSDLRKTLFQVMDVLIKTHPQDDPVYQFLDKKRAQGKPYYVYMTAGANKFLRIYYGRV
                     KEYLSSLPES"
BASE COUNT          448 a          301 c          320 g          463 t
ORIGIN      
        1 ttgaaaattt tatacaggtt gaaaattagg tatcaaaata agaccaccac accagtgctg
       61 gcctgaaaat attagtggat aattaagatt ctggaagaga tgaaagatat tctttcactc
      121 gtccgtagta gattcttaga aacttgttgg cacctgcagt catatagaca tagtaaggct
      181 tgccttgagc acgtttctta tctaagaact gataaacagg atcatcttgc ggatgcgttt
      241 taatcaggac atccattacc tgaaataaag tctttctaag atcagaggag cctcgtttgg
      301 aagttggaac acttttttgt tcataacttc ccgattcatt aacaccggga tctacaccgg
      361 caaatgcagt aatagcactt ttgtgagtga aacgggaaac atctccgatc tcagctatca
      421 actgaggacc aagtgaagtt ccaacccctt tcatagccat aacgacagga tattccggga
      481 gtttggatgc agtttcattc atgagagtgc gtagtgactc aacagttgta gaggcactat
      541 taagctggtc tacagcctgc ttaataataa gctttgtaat ggcatccttt gaaagtacag
      601 gaacaagttc ctttgctttt ccatagattt cctcagcttt ggattggctg aagttgtact
      661 tcttacgttt gcaccagttt tgataatggt cgataaagga attcagggac attttacgga
      721 cacagtctac atgccagtat gtagatgcaa aatcaaccca tttctggctg ccgtcactgc
      781 gtgcaggact gtcaaagtaa gtattgacac caggataggt ttggtcaagg atgccgataa
      841 gattattttt catagccgtc ttgtgtttta tgtaaaagcc gaactgacgg ttcatggttt
      901 tgagctgatt gcgtaattca tccataacat tatactgttt aagattttgc catttgtcaa
      961 gagtatatcg ggcaatctta acagcatctg ctttatcaga tttcacttta cgaagagaat
     1021 cattatcaaa gtctttgata agttttgggt taatggcact gacgaaaaga tttgcctctg
     1081 aaagctgatg agcgaggact tcataataac gccctgtgtg ctccatcacg attcgggact
     1141 caccttcaac agagttgatg agttctacaa gtgaattgat accactggct gtgtgtttga
     1201 tttcgaaagg tgcggaaaca atttcaccga aaggtcgcat gatagcaacc atactcttac
     1261 cttttgaaac atcgatacct actgcgttca taaattgtca ctccttaaga ttattgcaat
     1321 ggataagtac cagttttact cattgcctat tcaatctact gtggtgtgac gcgaatgcac
     1381 ctatggcgat tcaacctgca taaaacgaac gctgcgaatg aggagctggt tatcagtctt
     1441 aattacggac gcggagtcca agaaagtatt cgatataccg attgctccat cattatacag
     1501 cttaagcaac aagatggata attccttact gg
//
LOCUS       DBMDWL010000296         1526 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_39343_length_1526_cov_1.741053, whole genome shotgun sequence.
ACCESSION   DBMDWL010000296 DBMDWL010000000
VERSION     DBMDWL010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39343_length_1526_cov_1.741053"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="ACZPDN_12130"
     CDS             complement(<1..913)
                     /locus_tag="ACZPDN_12130"
                     /inference="COORDINATES: protein motif:HMM:NF048703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7309 domain-containing protein"
                     /protein_id="HIR1299402.1"
                     /translation="MATQATLEQWEELYDLAIEITKLKPWNSYWDMDLIAVETKKNEE
                     PNFVSIMGKGGTCTGISVYRGMEGYSDFCQISNDDYNVPATFVMSDQNCITCYWGNRD
                     EVDDDMYSIIKQLGLRFRGNGNWIYFKTFEKKSFPSLPNYGEVKMLIETYKGLIETLK
                     HEEYQESIFDKGDIIYTQKDEDNHWFSYTVPRPMEPERYNTILVNDSNLIDYFKNMDD
                     SDLELAIDMDYMMVPTNDAGFERPINPLAYIVFDLRNDQILHFDLIHPDQEDYEVIMN
                     NFLGAIEHFGKPKHVYARNPYILNWLDF"
     gene            complement(969..1445)
                     /locus_tag="ACZPDN_12135"
     CDS             complement(969..1445)
                     /locus_tag="ACZPDN_12135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1299403.1"
                     /translation="MFKTMRRFKQQLSQEKCIEILTNEPHGVLSLISDTGYPYGFPIT
                     HWYDEKTGNIYFHGAKEGHKMDCLKQCDKASFCVMDKGFKKDGEWAIHYQSVIVFGKI
                     KLVDDPNKVKEICTKLCLKFTNDQDYIDHELKYSGPSVQCFKLIPEYMTGKIIKES"
BASE COUNT          482 a          277 c          220 g          547 t
ORIGIN      
        1 taaaatcaag ccaatttaga atgtatggat ttctggcata tacatgttta ggtttaccaa
       61 aatgttcgat ggcccccaag aaattattca taatgacttc ataatcctct tgatctgggt
      121 gaattaaatc aaagtgtaaa atttgatcat ttcttaaatc aaatacaata tatgccaatg
      181 gattgatagg tctttcaaaa cctgcatcat ttgtaggaac catcatataa tccatatcga
      241 tggcaagttc taaatctgag tcatccatat ttttaaaata atcaattaga tttgaatcgt
      301 tgacaagaat cgtattatat cgttctggtt ccatgggtct aggtacggta taagaaaacc
      361 aatgattgtc ttcatctttt tgggtataga taatatcacc tttgtcaaaa atagattctt
      421 ggtattcttc atgtttaagt gtttcaatca atcccttgta ggtttcaatc aacatcttaa
      481 cttcaccata atttggaaga ctaggaaaac tcttcttttc aaaagttttg aaatagatcc
      541 agtttccatt tccacggaat cgaagaccta attgtttgat aatagaatac atatcatcat
      601 ctacttcatc acggtttccc caataacaag taatgcagtt ttgatcagac atcacaaatg
      661 ttgcaggtac gttatagtcg tcattgctga tttgacaaaa atcactatat ccttccatgc
      721 cacgatagac actgatacct gtgcaagtgc cacctttacc cataattgat acgaagttgg
      781 gttcttcatt tttctttgtt tctaccgcaa ttaaatccat gtcccaataa gagttccaag
      841 gctttaattt ggtgatttca atagctaaat catatagctc ttcccattgt tctaatgttg
      901 cttgtgttgc cataataatt cctcaacttt attatattaa tagtaacatt gaaggacatg
      961 aaactcaatt aagattcttt tatgattttt ccagtcatat attcagggat aagtttaaaa
     1021 cattgcacac taggtcctga atactttaat tcatgatcaa tataatcttg atcgttagta
     1081 aattttaggc agagcttagt acatatttct ttgactttat ttggatcatc aaccaacttg
     1141 atttttccaa atacaataac actctgataa tgaatagccc attcaccatc tttcttaaaa
     1201 cctttatcca tgacgcaaaa gctagcttta tcgcattgtt ttaaacaatc catcttgtgt
     1261 ccttctttag ccccatgaaa ataaatgttt ccagtctttt catcgtacca atgtgtgata
     1321 ggaaaaccat agggatatcc tgtatcactg atcaaagata gtactccatg aggttcattg
     1381 gtaagtattt caatacattt ttcttggctt aattgttgtt taaatcttcg cattgtctta
     1441 aacataggta tctcctttaa tgaaaaaggt gtaaattgaa ttacacctct aaagatttga
     1501 attcatgaat tcaatgattc cggctt
//
LOCUS       DBMDWL010000297         1511 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_39852_length_1511_cov_2.604255, whole genome shotgun sequence.
ACCESSION   DBMDWL010000297 DBMDWL010000000
VERSION     DBMDWL010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39852_length_1511_cov_2.604255"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(121..1086)
                     /locus_tag="ACZPDN_12140"
     CDS             complement(121..1086)
                     /locus_tag="ACZPDN_12140"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR1299404.1"
                     /translation="MEKGTKRHKGRVRSIKKSYWHTGQVKPREIYEAVDQQKKKDQTI
                     SVASVLKTIGVSRSGFYSYKKNENLPTQTQIRNEHLQKEIKKIHEGSYGIYGSPKITV
                     LLNKNGENVSQKHVYNLMHGMGIKARYVRHYTKTTVSEDYSSKMQNLLNRHFNPTKPN
                     TVWCTDITYIWTREDGFVYLTSVMDLFSRRIIAWVLTRTMDADKVLECLIKAKERRET
                     DHPIVIQSDRGVQYTSELYQRLTKGMVTSYSWKGTPWDNACIESFHALIKREWLQFYK
                     IDNYYGAYKIVFEYIEGFYNTTRIHSHCEYESPQNFEKQYLKHKH"
     gene            complement(1011..1307)
                     /locus_tag="ACZPDN_12145"
     CDS             complement(1011..1307)
                     /locus_tag="ACZPDN_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013677.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1299405.1"
                     /translation="MSRNSKAYDQEFKENVINYVLEHPEEKYTALGNKFGVHPTTIAG
                     WMKQYRSNDNQVIVRGSGNYASDEAKEIAKLKKELKDTKDALEVLKKAIGILGK"
BASE COUNT          416 a          276 c          217 g          602 t
ORIGIN      
        1 atcagattgt taaaagcgca aaaatagata atgaatgggt aaagaattat ttagatgaca
       61 tctggtatca tgaagtatgg tactatgtca agatttaaga caacttaaat atctgttttt
      121 ttaatgttta tgtttcaaat attgtttttc aaaattttgt ggagattcat attcacaatg
      181 tgagtgaatt cgtgttgtat tataaaatcc ttctatatat tcaaatacga ttttatatgc
      241 tccataataa ttatcaattt tataaaattg tagccattct ctttttatca aggcatgaaa
      301 agattcaatg cacgcattgt cccatggagt tcctttccat gaatagctag taaccatacc
      361 tttagttaat cgctggtata attctgatgt atactgtact ccgcggtcac tttggatcac
      421 aataggatga tcagtttcac gtctttcttt agcttttatt aagcattcta acaccttgtc
      481 cgcatccatt gttcgcgtta atacccatgc tataattctt cttgaaaata aatccattac
      541 acttgttaaa tatacaaatc catcttctct tgtccatata taagttatat ctgtacacca
      601 tacggtattt ggttttgttg gattaaaatg cctatttaat aggttctgca tcttagacga
      661 ataatcttca cttacagttg tttttgtgta atgtcgtaca taacgggctt ttattcccat
      721 accatgcatt aaattatata catgtttctg actgacgttt tcgccatttt tatttagtaa
      781 tactgttatt tttggtgatc catatatacc ataagatcct tcatgtattt tcttgatttc
      841 tttttgaaga tgctcatttc ttatttgtgt ctgagtaggt agattttcat tctttttata
      901 gctatagaaa cctgaacgac taaccccaat tgtttttagc acactggcta cggaaatagt
      961 ttgatccttt ttcttttgtt gatcaaccgc ttcgtagatt tcacggggct ttacttgccc
     1021 agtatgccaa tagctttttt taatacttct aacgcgtcct ttgtgtcttt tagttccttt
     1081 ttcaatttcg cgatttcttt agcttcatca cttgcataat taccgctgcc tctaacaata
     1141 acttgattgt cattgctgcg atattgtttc atccacccag caatcgttgt aggatgaaca
     1201 ccgaatttat ttccaagtgc agtgtatttt tcttctggat gttctaaaac ataattaata
     1261 acattttctt taaattcttg gtcataggct ttactgtttc ttgacatttt ttatactcct
     1321 ttgttattgt cttgacattg taccatatgc acaagaatct acaatatcca ggacagtcat
     1381 gttcaatatc tcatcctatt tttaatgtga ttggttcctt atcaaatcaa attcgagatg
     1441 ttttacctgt cctataaata tactagcatc aaatacctct tgcaccgcgg ttgacccatc
     1501 tgccgaaaat t
//
LOCUS       DBMDWL010000298         1506 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Holdemanella biformis isolate MAG1205
            NODE_40045_length_1506_cov_1.800000, whole genome shotgun sequence.
ACCESSION   DBMDWL010000298 DBMDWL010000000
VERSION     DBMDWL010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508304
            Sequence Read Archive: ERR10114001
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Holdemanella biformis (human gut metagenome)
  ORGANISM  Holdemanella biformis
            Bacteria; Bacillati; Bacillota; Erysipelotrichia;
            Erysipelotrichales; Erysipelotrichaceae; Holdemanella.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6525x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:10:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,431
            CDSs (total)                      :: 2,376
            Genes (coding)                    :: 2,365
            CDSs (with protein)               :: 2,365
            Genes (RNA)                       :: 55
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Holdemanella biformis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40045_length_1506_cov_1.800000"
                     /isolate="MAG1205"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:1735"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="ACZPDN_12150"
     CDS             complement(<1..490)
                     /locus_tag="ACZPDN_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR1299406.1"
                     /translation="MSNQKQNNRPRGPMGHGMRGGEKAKDFKGTMKKLLNYIKPFKFS
                     FLVGLLCAIVSVCIMVIGPKIQGNIITEIAQGFMNKVQGKGGIDFDAIQKTCALLLAI
                     YALSTAFSYLQGWLMSGVSNKMGYKLRKEMNQKIDKLPLSYFDKNSHGDILSRFTNDV
                     DTL"
     gene            complement(483..1424)
                     /locus_tag="ACZPDN_12155"
                     /pseudo
     CDS             complement(483..1424)
                     /locus_tag="ACZPDN_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864918.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          463 a          324 c          236 g          483 t
ORIGIN      
        1 ctagagtatc tacatcatta gtaaagcgag ataaaatgtc tccatgactg tttttatcaa
       61 agtaagatag tggaagctta tcaatctttt gattcatttc tttacgaagc ttgtatccca
      121 ttttattcga gacaccactc atcaaccagc cttgaagata cgagaaggct gtactcaaag
      181 cataaatcgc aagtaataaa gcacatgttt tttgaatcgc atcaaaatca atgccacctt
      241 taccttgcac tttattcata aagccttgcg caatttcagt aataatattt ccttgaatct
      301 taggcccaat caccatgatg caaacggaaa caatggcaca taatagacca actagaaatg
      361 agaatttaaa gggtttgatg tagtttaaaa gcttcttcat cgttcccttg aaatcttttg
      421 ccttttcgcc gccacgcata ccatggccca taggacctct tggacgatta ttttgtttct
      481 gattagacat tggcaagttc ctcctttgtc aattggctag acgcaatttg ttgataaacc
      541 tcacaagatt tcatcaattg ggcatgtgta cctttgccaa ccatctttcc ttcatcgagt
      601 acaatgattt gatccgcatg taaaatacta gaaatacgtt gtgccacaag taatactgta
      661 ctctttgttt ctttacaaga ctttgccaaa gcctctctta gtttagcatc cgtcttaaaa
      721 tccaaggcac taaagctatc atcaaaaata tagatttcag gctttcttac aacggcacgg
      781 gcaatggaaa gtctttgtct ttgtccgcct gaaacatttg ttccgccctg attgatttca
      841 ctcttgattc cttctggctt tttagaaaca aactccgtac tttgagaaat agataatgca
      901 ttagaaatat catccttgct tgcatctttc tttgaataca ataagttcga ttcaatatca
      961 cccgatagta acatcccctt ttgtggaaca tagccaatac gacttcttag atctgcctgt
     1021 ttcacattcc gaatatcaac tccatccact aaaatcgaac cgcttgttac atcatagaaa
     1081 cgtgggacta aattaatcaa tgttgattta ccagatccag tacttccaat aaaggctgtt
     1141 gtttcgcctg gtttggtttc aaaactaata tcactcaaca cttcttcatc ggcacctgga
     1201 tatttaaacg atacattttt aaaagagaca acaccacgca tagaagatga gaaatccata
     1261 ggatttgatg gatcttttat agtaatttca ctacttaaga cttcatgaat acgcaaagca
     1321 cttacatttg ctcgaggaag cataattgtg accatcgtaa tcattaaaaa ggccatgata
     1381 acttgcatcg catactgcat aaaggccatg atactaccaa cttgatgtaa attcatctta
     1441 taacatttgc ttctaattca tagcacgtgt gtcttatagt gtgtcgagca ggatcaaatt
     1501 tcatcc
//
